title: [An experimental study on the regulation of pulmonary arterial remodeling by protein kinase C in chronic hypoxic rats].
abstract: OBJECTIVE
  To investigate the effect of protein kinase C regulating pulmonary arterial remodeling in chronic hypoxic rats.
  
  
  METHODS
  Electron microscope, radioactivity, immunohistochemistry and image analyser were used.
  
  
  RESULTS
  (1) Mean pulmonary arterial pressure (mPAP) and weight ratio of RV to LV + S were significantly higher than that of control group (P < 0.01). (2) WA/TA and SMC were significantly higher than that of control group (P < 0.01). Electron microscopy showed the proliferation of smooth muscle cells and the disposition of collagenous fiber in pulmonary arterioles induced by hypoxia. (3) The total, cytosolic, particulate fraction PKC activity and the ratio of particulate fraction to total PKC activity were significantly higher than that of control group (P < 0.01). (4) Expression of PKC, collagen I were significantly higher than that of control group (P < 0.01), the difference of collagen III was not significant between two groups (P > 0.05). (5) There were good correlation between the total, particulate fraction PKC activity, the ratio of particulate fraction to total PKC activity, expression of PKC and SMC, collagen I in pulmonary arterioles.
  
  
  CONCLUSIONS
  The PKC regulates the proliferation of pulmonary artery smooth muscle cells and expression of pulmonary arterial collagen in chronic hypoxic rats, which may play an important role in the pathogenesis of pulmonary hypertension and structural remodeling of pulmonary arteries.
authors.affiliation: Department of Respirology, The First Affiliated Hospital, Wenzhou Medical College, Institute of Cor Pulmonale, Wenzhou 325000, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Shao-Xian
authors.initials: SX
authors.lastname: Chen
authors.forename: Liang-Xing
authors.initials: LX
authors.lastname: Wang
authors.forename: Yan-Fan
authors.initials: YF
authors.lastname: Chen
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Xie
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D006976:Hypertension, Pulmonary / Q000378:metabolism / Q000503:physiopathology; D000860:Hypoxia / Q000378:metabolism* / Q000503:physiopathology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D011651:Pulmonary Artery / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003094:Collagen; D011493:Protein Kinase C