title: Nonthermal dielectric-barrier discharge plasma-induced inactivation involves oxidative DNA damage and membrane lipid peroxidation in Escherichia coli.
abstract: Oxidative stress leads to membrane lipid peroxidation, which yields products causing variable degrees of detrimental oxidative modifications in cells. Reactive oxygen species (ROS) are the key regulators in this process and induce lipid peroxidation in Escherichia coli. Application of nonthermal (cold) plasma is increasingly used for inactivation of surface contaminants. Recently, we reported a successful application of nonthermal plasma, using a floating-electrode dielectric-barrier discharge (FE-DBD) technique for rapid inactivation of bacterial contaminants in normal atmospheric air (S. G. Joshi et al., Am. J. Infect. Control 38:293-301, 2010). In the present report, we demonstrate that FE-DBD plasma-mediated inactivation involves membrane lipid peroxidation in E. coli. Dose-dependent ROS, such as singlet oxygen and hydrogen peroxide-like species generated during plasma-induced oxidative stress, were responsible for membrane lipid peroxidation, and ROS scavengers, such as α-tocopherol (vitamin E), were able to significantly inhibit the extent of lipid peroxidation and oxidative DNA damage. These findings indicate that this is a major mechanism involved in FE-DBD plasma-mediated inactivation of bacteria.
authors.affiliation: Surgical Infection Program, Department of Surgery, Drexel University College of Medicine, 245 N. 15th Street, Room NCB-7150, Mail 413, DUCOM, Philadelphia, PA 19102, USA. Suresh.Joshi@Drexelmed.edu
authors.forename: Suresh G
authors.initials: SG
authors.lastname: Joshi
authors.forename: Moogega
authors.initials: M
authors.lastname: Cooper
authors.forename: Adam
authors.initials: A
authors.lastname: Yost
authors.forename: Michelle
authors.initials: M
authors.lastname: Paff
authors.forename: Utku K
authors.initials: UK
authors.lastname: Ercan
authors.forename: Gregory
authors.initials: G
authors.lastname: Fridman
authors.forename: Gary
authors.initials: G
authors.lastname: Friedman
authors.forename: Alexander
authors.initials: A
authors.lastname: Fridman
authors.forename: Ari D
authors.initials: AD
authors.lastname: Brooks
mesh_terms: D004249:DNA Damage; D004203:Disinfection / Q000379:methods*; D004560:Electricity; D004926:Escherichia coli / Q000378:metabolism*; D015227:Lipid Peroxidation; D008563:Membrane Lipids / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D017382:Reactive Oxygen Species