title: A novel adsorbent for protein chromatography: supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles.
abstract: The aim of this study is to prepare supermacroporous cryogels embedded with Cu(2+)-attached sporopollenin particles (Cu(2+)-ASP) having large surface area for high protein adsorption capacity. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Cu(2+)-ASP was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for affinity purification of human serum albumin (HSA). Firstly, Cu(2+) ions were attached to sporopollenin particles (SP), then the supermacroporous PHEMA cryogel with embedded Cu(2+)-ASP was produced by free radical polymerization using N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Embedded particles (10 mg) in PHEMA-based cryogel column were used in the adsorption/desorption of HSA from aqueous solutions. Optimum conditions of adsorption experiments were performed at pH 8.0 phosphate buffer, with flow rate of 0.5 mL/min, and at 5°C. The maximum amount of HSA adsorption from aqueous solution was very high (677.4 mg/g SP) with initial concentration 6 mg/mL. It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Cu(2+)-ASP in PHEMA cryogel without significant loss of adsorption capacity.
authors.affiliation: Aksaray University, Faculty of Arts and Science, Chemistry Department, Aksaray, Turkey.
authors.forename: Mahmut
authors.initials: M
authors.lastname: Erzengin
authors.forename: Nuri
authors.initials: N
authors.lastname: Ünlü
authors.forename: Mehmet
authors.initials: M
authors.lastname: Odabaşı
mesh_terms: D000327:Adsorption; D001704:Biopolymers / Q000737:chemistry*; D002338:Carotenoids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature
chemical_list: D001704:Biopolymers; D020100:Hydrogels; D007093:Imidazoles; D008689:Methacrylates; D011506:Proteins; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C009800:sporopollenin; D002338:Carotenoids; C005044:hydroxyethyl methacrylate; D003300:Copper