title: [The protection effect of BDNF mediated by adenovirus to the serum free cultured SH-SY5Y cells].
abstract: OBJECTIVE
  To study the protective effect of adenovirus mediated brain derived neurotrophic factors on nerve injury.
  
  
  METHODS
  SH-SY5Y cells were infected by the recombinant Ad-BDNF. In the serum-free condition, the morphological changes were observed, and MTT method was used to examine the trophic effects of different dosages of Ad-BDNF. The whole cells DNA of infected and control groups were extracted to detect the DNA ladder--the marker of apoptosis.
  
  
  CONCLUSIONS
  The adenoviruses mediated BDNF gene promoted the cell survival and differentiation. It also inhibited the serum-deprived induced cell apoptosis.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D018014:Gene Transfer Techniques; D006801:Humans; D009474:Neurons
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D016895:Culture Media, Serum-Free