title: Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy.
abstract: Corneodesmosin, a protein known to be present in the stratum corneum (SC), plays an important role in its physical integrity. Here, a specific antibody to corneodesmosin was tethered via a flexible linker to an atomic force microscopy tip, and the interaction forces between this tip and the surface of the SC were successfully measured. Using the recently developed technique of simultaneous topography and recognition imaging, we were able to map the distribution of corneodesmosin on the surface of the SC at the nanoscale.
authors.affiliation: Institute for Biophysics, University of Linz, Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Rankl
authors.forename: Rong
authors.initials: R
authors.lastname: Zhu
authors.forename: Gustavo S
authors.initials: GS
authors.lastname: Luengo
authors.forename: Mark
authors.initials: M
authors.lastname: Donovan
authors.forename: Nawel
authors.initials: N
authors.lastname: Baghdadli
authors.forename: Peter
authors.initials: P
authors.lastname: Hinterdorfer
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D004817:Epidermis / Q000378:metabolism* / Q000648:ultrastructure; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D018625:Microscopy, Atomic Force / Q000379:methods*; D011485:Protein Binding / Q000502:physiology; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; C109656:CDSN protein, human; D003432:Cross-Linking Reagents; D006023:Glycoproteins; D007074:Immunoglobulin G; D036341:Intercellular Signaling Peptides and Proteins