title: Analysis of thioredoxin peroxidase as a promising antigen for diagnosis of Fasciola gigantica infection: a preliminary study.
abstract: Buffalo fasciolosis induced by Fasciola gigantica causes important economic losses in tropical areas of Asia. Detection of prepatent infection is essential to control this disease. Classical tools such as coprology, necroscopy or ELISA based on crude extracts from F. gigantica are poorly sensitive or specific. Purified antigens could be used to increase these parameters. Western blot analysis and mass spectrometry of a fraction of F. gigantica excretory-secretory products obtained by gel filtration showed that thioredoxin peroxidase could be a potential antigen for serodiagnosis: it was recognized from the 2nd week after infection, by all buffalo experimentally or naturally infected with F. gigantica but not by healthy animals.
authors.affiliation: College of Animal Science and Technology, Guangxi University, 530005 Nanning, China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Zhang
authors.forename: Hélène
authors.initials: H
authors.lastname: Rogniaux
authors.forename: Weiyi
authors.initials: W
authors.lastname: Huang
authors.forename: Alain
authors.initials: A
authors.lastname: Chauvin
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Moreau
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D001208:Asia; D015153:Blotting, Western / Q000379:methods; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005209:Fasciola / Q000254:growth & development / Q000302:isolation & purification*; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D015801:Helminth Proteins / Q000276:immunology; D054464:Peroxiredoxins / Q000378:metabolism*; D012698:Serologic Tests
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D054464:Peroxiredoxins