title: [Expression of human papillomavirus type 16/18 in human cervical carcinomas by the quantum dot fluorescent in-situ hybridization].
abstract: OBJECTIVE
  To investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients.
  
  
  METHODS
  A total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed.
  
  
  RESULTS
  The positive rate for HPV16/18 by QD-FISH was 88.8% (71/80), higher than that (80.0%) by CISH, however, the result was statistically not significant (P=0.127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage.
  
  
  CONCLUSIONS
  The sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.
authors.affiliation: Department of Pathology, Hubei Women and Children Health Care Hospital, Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Jun-bo
authors.initials: JB
authors.lastname: Hu
authors.forename: Hong-lei
authors.initials: HL
authors.lastname: Chen
authors.forename: Bei-yun
authors.initials: BY
authors.lastname: Li
authors.forename: He-shun
authors.initials: HS
authors.lastname: Xia
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D002863:Chromogenic Compounds; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology
chemical_list: D002863:Chromogenic Compounds