title: Expression of pluripotency genes in buffalo (Bubalus bubalis) amniotic fluid cells.
abstract: Recent findings suggest that mammalian amniotic fluid (AF) is a source of multipotent stem cells (SCs), which can be used in regenerative medicine and assisted reproduction. We report the isolation, culture and characterization of amniotic fluid-derived cells from pregnant water buffalo uterus. These undifferentiated AF cells expanded without feeder layer over a period of 100 days up to passages 20 and the expression of alkaline phosphatase (AP), Oct-4, Nanog and Sox-2, GAPDH and β-actin could be detected by RT-PCR. The cells exhibited uniform morphology and normal chromosome number. The up-regulation or down-regulation of transcription factors of each gene varied with passage number. We conclude that putative bubaline AF cells can be cultured and maintained in vitro for a prolonged period and offer a potential source of multipotent cells for applications including assisted reproduction in buffalo.
authors.affiliation: Buffalo Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, Haryana, India. psyadav@scientist.com
authors.forename: P S
authors.initials: PS
authors.lastname: Yadav
authors.forename: A
authors.initials: A
authors.lastname: Mann
authors.forename: V
authors.initials: V
authors.lastname: Singh
authors.forename: S
authors.initials: S
authors.lastname: Yashveer
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.forename: I
authors.initials: I
authors.lastname: Singh
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D002478:Cells, Cultured; D015925:Cryopreservation; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D011247:Pregnancy