title: Polymorphisms of caprine GDF9 gene and their association with litter size in Jining Grey goats.
abstract: The exons 1, 2 and flanking region of growth differentiation factor 9 (GDF9) gene in five randomly selected does of Jining Grey, Boer and Liaoning Cashmere goats were amplified and analyzed. Thirteen nucleotide differences were identified in GDF9 gene between sheep (AF078545) and goats. Four SNPs (G3288A in intron 1, G423A, A959C [Gln320Pro] and G1189A [Val397Ile] in exon 2) were detected in four goat breeds with different prolificacy, in which G3288A was a new SNP in goats. The results showed that loci 3288, 423 and 1189 in Boer goats, loci 3288 and 423 in Guizhou White goats, loci 423 and 1189 in Liaoning Cashmere goats were all in complete linkage disequilibrium (D' = 1, r (2) = 1), respectively. In moderate (Boer goat) and low prolificacy (Liaoning Cashmere goat) breeds, linkage analysis indicated that there were more fervent linkage disequilibrium among loci 3288, 423 and 1189 than high prolificacy (Jining Grey and Guizhou White goats) breeds. For the 959 locus, the genotype distribution showed obvious difference between high prolificacy breeds and moderate or low prolificacy breeds (P < 0.05 or P < 0.01). The Jining Grey goat does with genotype CC or AC had 0.81 (P < 0.01) or 0.63 (P < 0.01) kids more than those with genotype AA, respectively. The present study preliminarily showed an association between allele C at 959 locus of GDF9 gene and high litter size in Jining Grey goats. These results provide further evidence that the GDF9 gene may be significantly correlated with high prolificacy in goats.
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Feng
authors.forename: C X
authors.initials: CX
authors.lastname: Geng
authors.forename: X Z
authors.initials: XZ
authors.lastname: Lang
authors.forename: M X
authors.initials: MX
authors.lastname: Chu
authors.forename: G L
authors.initials: GL
authors.lastname: Cao
authors.forename: R
authors.initials: R
authors.lastname: Di
authors.forename: L
authors.initials: L
authors.lastname: Fang
authors.forename: H Q
authors.initials: HQ
authors.lastname: Chen
authors.forename: X L
authors.initials: XL
authors.lastname: Liu
authors.forename: N
authors.initials: N
authors.lastname: Li
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002681:China; D017931:DNA Primers / Q000378:metabolism; D005787:Gene Frequency; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics; D006041:Goats / Q000235:genetics*; D055429:Growth Differentiation Factor 9 / Q000235:genetics*; D006720:Homozygote; D016018:Least-Squares Analysis; D015810:Linkage Disequilibrium / Q000235:genetics; D008098:Litter Size / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D055429:Growth Differentiation Factor 9