title: [The effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture].
abstract: OBJECTIVE
  To study the effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture; to discuss the effect of basic fibroblast growth factor in periodontal regeneration.
  
  
  METHODS
  Human periodontal ligament fibroblasts were cultured and stimulated by basic fibroblast growth factor (0.1, 1.0, 10.0 ng x mL(-1)) for 24, 48, 72 h respectively, and then mRNA expression of beta1 integrin subunit was assessed by fluorescent quantitative real-time reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Basic fibroblast growth factor enhanced the mRNA expression of beta1 integrin subunit, and there was optimal effect when the concentration of basic fibroblast growth factor was 1.0 ng x mL(-1) at 24, 48, 72 h respectively; the mRNA expression of beta1 integrin subunit at 72 h was higher than that at 24, 48 h.
  
  
  CONCLUSIONS
  Basic fibroblast growth factor can strengthen human periodontal ligament fibroblasts' adhesion and may be one of important factors which participate in the periodontal regeneration.
authors.affiliation: Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Chong-tao
authors.initials: CT
authors.lastname: Lin
authors.forename: Bo
authors.initials: B
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2; D005347:Fibroblasts; D006801:Humans; D019012:Integrin beta1; D010513:Periodontal Ligament; D012333:RNA, Messenger; D012038:Regeneration
chemical_list: D019012:Integrin beta1; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2