title: PU.1 directly regulates retinoic acid-induced expression of RIG-G in leukemia cells.
abstract: RIG-G is a retinoic acid- or interferon-induced gene with potential anti-proliferation function. However, the mechanism underlying ATRA-induced RIG-G induction is not completely understood. Here, we demonstrate that ATRA up-regulates the expression of PU.1, which in turn directly binds to the promoter and increases the expression of RIG-G gene. Luciferase reporter assay and electrophoretic mobility shift assay reveal that PU.1 preferentially binds to one of the two putative binding sites on the RIG-G promoter. Moreover, silencing of PU.1 by shRNA markedly inhibited ATRA- but not IFNα-induced expression of RIG-G. These data provide new insight into the mechanism of ATRA-induced RIG-G expression.
authors.affiliation: Department of Pathophysiology and Chemical Biology, Division of Shanghai Universities E-Institutes, Key laboratory of Cell Differentiation and Apoptosis of Minister of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Gu
authors.forename: Chuan-Xu
authors.initials: CX
authors.lastname: Liu
authors.forename: Shao-Fang
authors.initials: SF
authors.lastname: Wu
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.forename: Han-Zhang
authors.initials: HZ
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Hu-Chen
authors.initials: HC
authors.lastname: Zhou
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D015534:Trans-Activators / Q000502:physiology*; D015533:Transcriptional Activation / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D016898:Interferon-alpha; D047908:Intracellular Signaling Peptides and Proteins; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D015534:Trans-Activators; D014212:Tretinoin; D000099232:Proto-Oncogene Protein Spi-1; C116468:IFIT3 protein, human