title: Gene identification in black cohosh (Actaea racemosa L.): expressed sequence tag profiling and genetic screening yields candidate genes for production of bioactive secondary metabolites.
abstract: Black cohosh (Actaea racemosa L., syn. Cimicifuga racemosa, Nutt., Ranunculaceae) is a popular herb used for relieving menopausal discomforts. A variety of secondary metabolites, including triterpenoids, phenolic dimers, and serotonin derivatives have been associated with its biological activity, but the genes and metabolic pathways as well as the tissue distribution of their production in this plant are unknown. A gene discovery effort was initiated in A. racemosa by partial sequencing of cDNA libraries constructed from young leaf, rhizome, and root tissues. In total, 2,066 expressed sequence tags (ESTs) were assembled into 1,590 unique genes (unigenes). Most of the unigenes were predicted to encode primary metabolism genes, but about 70 were identified as putative secondary metabolism genes. Several of these candidates were analyzed further and full-length cDNA and genomic sequences for a putative 2,3 oxidosqualene cyclase (CAS1) and two BAHD-type acyltransferases (ACT1 and HCT1) were obtained. Homology-based PCR screening for the central gene in plant serotonin biosynthesis, tryptophan decarboxylase (TDC), identified two TDC-related sequences in A. racemosa. CAS1, ACT1, and HCT1 were expressed in most plant tissues, whereas expression of TDC genes was detected only sporadically in immature flower heads and some very young leaf tissues. The cDNA libraries described and assorted genes identified provide initial insight into gene content and diversity in black cohosh, and provide tools and resources for detailed investigations of secondary metabolite genes and enzymes in this important medicinal plant.
authors.affiliation: Fischell Department of Bioengineering, Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Spiering
authors.forename: Lori A
authors.initials: LA
authors.lastname: Urban
authors.forename: Donald L
authors.initials: DL
authors.lastname: Nuss
authors.forename: Vivek
authors.initials: V
authors.lastname: Gopalan
authors.forename: Arlin
authors.initials: A
authors.lastname: Stoltzfus
authors.forename: Edward
authors.initials: E
authors.lastname: Eisenstein
mesh_terms: D031885:Cimicifuga / Q000235:genetics / Q000378:metabolism*; D020224:Expressed Sequence Tags; D019751:Intramolecular Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D010940:Plant Proteins; D019751:Intramolecular Transferases; C021924:lanosterol synthase; C021923:cycloartenol synthase