title: Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain.
abstract: We designed an efficient transformation system for Candida guilliermondii based on a ura5 ATCC 6260 derived recipient strain and a URA5 recyclable selection marker. This "URA5 blaster" disruption system represents a powerful tool to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.
authors.affiliation: Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François Rabelais, Tours, France.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Millerioux
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Simkin
authors.forename: Emeline
authors.initials: E
authors.lastname: Marais
authors.forename: Andriy A
authors.initials: AA
authors.lastname: Sibirny
authors.forename: Thierry
authors.initials: T
authors.lastname: Noël
authors.forename: Joël
authors.initials: J
authors.lastname: Crèche
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
mesh_terms: D002175:Candida / Q000235:genetics*; D005800:Genes, Fungal; D005827:Genetics, Microbial / Q000379:methods*; D006801:Humans; D016254:Mutagenesis, Insertional / Q000379:methods*; D012641:Selection, Genetic; D014170:Transformation, Genetic