title: Modulation of i-motif thermodynamic stability by the introduction of UNA (unlocked nucleic acid) monomers.
abstract: The influence of acyclic RNA derivatives, UNA (unlocked nucleic acid) monomers, on i-DNA thermodynamic stability has been investigated. The 22nt human telomeric fragment was chosen as the model sequence for stability studies. UNA monomers modulate i-motif stability in a position-depending manner. The largest destabilization is observed for position C14, while UNA placed in position A12 causes significant increase of i-DNA thermodynamic stability. CD curves of UNA-modified variants imply no structural changes relative to the native i-motif.
authors.affiliation: Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
authors.forename: Anna
authors.initials: A
authors.lastname: Pasternak
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006801:Humans; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D016615:Telomere / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D012313:RNA; D004247:DNA