title: Optimization protocol for amyloid-β peptides detection in human cerebrospinal fluid using SELDI TOF MS.
abstract: OBJECTIVE
  The aim of the present work was to set up an optimized protocol for human cerebrospinal fluid amyloid-β (Aβ) profiling.
  
  
  METHODS
  We devised an immunoproteomic assay that employs monoclonal antibodies (mAbs) on Preactivated Surface (PS20) chip array followed by SELDI TOF MS. A comparison of a number of factors was performed, and the impact of these differences was noted. Each variable was tested using in parallel two different mAbs, 6E10 and 4G8. In addition, we tested whether the combined use of these two mAbs could improve the capture of N and C-terminally truncated Aβ peptides and then the quality of spectra.
  
  
  RESULTS
  The best results were obtained using a mixture of Aβ mAbs (0.125 μg/μL 6E10+4G8): 15 Aβ peptides (including 3 N-terminally truncated forms) were detected.
  
  
  CONCLUSIONS
  This approach has many potential advantages in speed, sensitivity and economy of reagents and could be helpful in order to define the role played by specific Aβ truncated forms in cognitive decline.
authors.affiliation: Proteomics Unit, IRCCS Centro S. Giovanni di Dio-FBF, Brescia, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Albertini
authors.forename: Anna
authors.initials: A
authors.lastname: Bruno
authors.forename: Anna
authors.initials: A
authors.lastname: Paterlini
authors.forename: Simone
authors.initials: S
authors.lastname: Lista
authors.forename: Luisa
authors.initials: L
authors.lastname: Benussi
authors.forename: Cristina
authors.initials: C
authors.lastname: Cereda
authors.forename: Giuliano
authors.initials: G
authors.lastname: Binetti
authors.forename: Roberta
authors.initials: R
authors.lastname: Ghidoni
mesh_terms: D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal