title: Electrochemical control of adsorption dynamics of surface layer proteins on gold.
abstract: The kinetics and mechanism of the adsorption of the surface layer proteins of Lysinibacillus sphaericus CCM2177 on gold depend on the charging conditions of the electrochemical double layer and the addition of Ca(2+) ions. The electrical and mass charging was monitored by an in situ electrochemical quartz microbalance. Adsorption and monolayer formation of the protein molecules occur in the positive potential region where solvated anions form the electrochemical double layer. The crystalline character of the surface layer was diagnosed by an atomic force microscope. Negative of the point of zero charge, multilayer island structures were found.
authors.affiliation: University of Vienna, Department of Physical Chemistry, Währinger Strasse 42, A-1090 Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Zafiu
authors.forename: Günter
authors.initials: G
authors.lastname: Trettenhahn
authors.forename: Dietmar
authors.initials: D
authors.lastname: Pum
authors.forename: Uwe Bernd
authors.initials: UB
authors.lastname: Sleytr
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kautek
mesh_terms: D000327:Adsorption; D001406:Bacillaceae / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D006046:Gold; D002118:Calcium