title: [Effect of vasonatrin peptide on the expression of C-type natriuretic peptide receptor in hypoxic rat hearts].
abstract: OBJECTIVE
  To investigate the effect of vasonatrin peptide (VNP) on the expression of C-type natriuretic peptide receptor (NPR-C) in hypoxic rat hearts.
  
  
  METHODS
  Rats were divided randomly into three groups: control group, hypoxia group(3-28 d) and VNP (25-75 microg/kg per day) + hypoxia group. The plasma concentration of atrial natriuretic peptide (ANP) in rats was measured by the means of radioimmunoassay. Furthermore, quantitative PCR was used to examine the NPR-C mRNA level in rat hearts.
  
  
  RESULTS
  The plasma concentration ANP in rats was significantly higher than that of control group, and VNP (75 microg/kg per day) made it more higher. Hypoxia for 3 day of had no significant effect on the NPR-C mRNA level in rat hearts, while hypoxia for 7-28 d significantly increased the level of NPR-C mRNA in a time dependent manner. VNP (50-75 microg/kg per day) significantly reduced the NPR-C mRNA level in rat hearts in a dose dependent manner.
  
  
  CONCLUSIONS
  VNP increases the plasma concentration of ANP in hypoxic rats. Hypoxia can increase expression of NPR-C in rat hearts significantly, which can be inhibited by VNP.
authors.affiliation: Department of Physiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Miao-zhang
authors.initials: MZ
authors.lastname: Zhu
authors.forename: Bao-ying
authors.initials: BY
authors.lastname: Chen
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000494:pharmacology; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D020098:Natriuretic Peptide, C-Type / Q000378:metabolism; D045265:Natriuretic Peptides / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*
chemical_list: D045265:Natriuretic Peptides; D020098:Natriuretic Peptide, C-Type; C068986:ventricular natriuretic peptide, eel; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor