title: [Analysis of two-dimension gel electrophoresis of human large cell lung cancer cell lines with different metastasis potentials].
abstract: BACKGROUND
  Metastasis is not only the malignant characteristics of lung can- cer, but also the chief cause of failure to cure and high mortality of lung cancer. To better explore and understand the mechanism of lung cancer metastasis and to search for potential markers for early diagnosing and reversing lung cancer metastasis, differential proteomic analysis is conducted in two human large cell lung cancer cell lines with high metastasis potentials (L9981) and low metastasis potentials (NL9980) by two-dimension gel electrophoresis (2-DE).
  
  
  METHODS
  The total proteins of the two cell lines were separated by immobilized pH gradient (IPG)-based 2-DE. The differentially expressed proteins of the two cell lines were analyzed using image analysis software.
  
  
  RESULTS
  A high resolution and reproducible 2-DE image was successfully obtained. Average deviations for protein position in IEF direction were (0.858±0.076)mm and (1.514±0.127)mm in SDS-PAGE direction. The relative standard deviation for protein volume was (12.06±0.580)% in L9981 and (12.22±0.640)% in NL9980. The average total number of protein spots was 902±169 in L9981 cells and 941±173 in NL9980 cells in three repeated experiments. Image analysis of siliver-stained 2-DE image revealed that 4 protein spots had significant differential expressions in L9981 and NL9980 (student's t-test, P < 0.05). Fifteen protein spots were only detected in L9981, and 27 protein spots were only detected in NL9980.
  
  
  CONCLUSIONS
  The results in this study suggest that an obviously differential proteomic expression exists between the human high- and low-metastatic large cell lung cancer cell lines. It will be helpful to further understand the molecular mechanisms of lung cancer invasion and metastasis, and provide new experimental evidence for searching metastatic-related molecule of lung cancer.
authors.affiliation: The Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.forename: Youlin
authors.initials: Y
authors.lastname: Deng
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Chen
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Lunxu
authors.initials: L
authors.lastname: Liu
authors.forename: Guowei
authors.initials: G
authors.lastname: Che