title: Umbilical cord blood concentrations of IL-6, IL-8, and MMP-8 in pregnancy complicated by preterm premature rupture of the membranes and histological chorioamnionitis.
abstract: OBJECTIVE
  To determine whether umbilical cord blood concentrations of interleukin-6 (IL-6), interleukin-8 (IL-8), and matrix metalloproteinase-8 (MMP-8) are of value in the diagnosis of histological chorioamnionitis (HCA) and funisitis in patients with preterm premature rupture of membranes (PPROM).
  
  
  METHODS
  Department of Obstetrics and Gynaecology, Charles University in Prague, Faculty of Medicine Hradec Kralove, University Hospital Hradec Kralove, Czech Republic.
  
  
  METHODS
  We compared umbilical cord blood IL-6, IL-8, and MMP-8 concentrations in 83 women with PPROM between 24th and 36th gestational weeks with the presence and the absence of HCA/funisitis using nonparametric tests (Mann-Whitney U test), given the non-normal distribution of analyte. Comparisons of proportions were performed the D'Agostino and Pearson omnibus normality test and the Shapiro-Wilk test.
  
  
  RESULTS
  Patients with HCA had a significantly higher median umbilical cord blood IL-6 concentration than patients without histological signs of inflammation (12.0 pg/mL [2.1-138.3] versus 2.7 pg/mL [0.1-12.4]; p=0.004) but did not have significantly higher median umbilical cord IL-8 (29.9 pg/mL [14.0-186.3]; versus 18.9 pg/mL [7.9-89.4]; p=0.13) and MMP-8 (2.9 pg/mL [0.5-25.2] versus 0.5 ng/mL [0.5-7.9]; p=0.18). Patients with HCA and funisitis had a significantly higher median umbilical cord blood IL-6 (222 pg/mL [95.3-411.7] versus 6.1 pg/mL [1.3-18.5]; p<0.0001) and IL-8 (20.9 pg/mL [8.4-37.7] versus 190.7 pg/mL [83.8-554.2]; p=0.0004) concentration than patients with HCA alone. Differences were not found in MMP-8 concentrations (3.7 ng/mL [0.5-21.4] versus 2.4 ng/mL [0.5-88.1]; p=0.7).
  
  
  CONCLUSIONS
  HCA was associated with a significant increase in umbilical cord blood IL-6 concentration. In patients with HCA and funisitis, umbilical cord blood IL-6 and IL-8 were significantly higher than those without histological signs of inflammation.
authors.affiliation: Department of Clinical Immunology and Allergy, Charles UniversityPrague, Czech Republic.
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.forename: Marcela
authors.initials: M
authors.lastname: Drahosova
authors.forename: Helena
authors.initials: H
authors.lastname: Hornychova
authors.forename: Vojtech
authors.initials: V
authors.lastname: Tambor
authors.forename: Ivana
authors.initials: I
authors.lastname: Musilova
authors.forename: Jindrich
authors.initials: J
authors.lastname: Tosner
authors.forename: Eva
authors.initials: E
authors.lastname: Flidrova
authors.forename: Marian
authors.initials: M
authors.lastname: Kacerovsky
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D002821:Chorioamnionitis / Q000097:blood / Q000175:diagnosis*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005322:Premature Rupture of Fetal Membranes / Q000097:blood / Q000175:diagnosis*; D005333:Fetus; D006801:Humans; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000097:blood*; D011247:Pregnancy
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D016209:Interleukin-8; D020784:Matrix Metalloproteinase 8