title: Gene expression profile in the liver tissue of geese after overfeeding.
abstract: Geese form a fatty liver after feeding on a carbohydrate-rich diet, possibly as an evolutionary adaptation to accumulate reserves for migration. To gain insight into the gene-regulation processes of hepatic steatosis in geese, we examined the profile of transcriptional expression in goose fatty liver and control liver by suppression subtractive hybridization and measured the levels of serum biochemical variables. We found 107 genes whose expression was different between the treatment and control groups. The main functions of these genes are metabolic processes, including the metabolism of carbohydrates, amino acids, and lipids. Twenty-four genes were classified using the Kyoto Encyclopaedia of Genes and Genomes pathways. Twelve genes that related to metabolic and cellular processes were confirmed by quantitative RT-PCR. A specific positive effect of feeding was observed on the expression of genes involved mainly in unsaturated fatty acids and triglyceride synthesis, and a negative effect was observed on genes involved in β-oxidation, cholesterol metabolism, and glycolysis. The results could serve as an important reference for the development of goose breeding for fatty liver production and human liver disease research.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, P. R. China.
authors.forename: L H
authors.initials: LH
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Meng
authors.forename: X J
authors.initials: XJ
authors.lastname: Duan
authors.forename: G Q
authors.initials: GQ
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: D Q
authors.initials: DQ
authors.lastname: Gong
mesh_terms: D000821:Animal Feed; D000822:Animal Husbandry; D000818:Animals; D001835:Body Weight; D004435:Eating / Q000502:physiology*; D005777:Geese / Q000502:physiology*; D020869:Gene Expression Profiling / Q000662:veterinary*; D008099:Liver / Q000378:metabolism*; D008297:Male