title: Neutrophils infiltration is early event of an oral mucosal xenotransplantation model.
abstract: BACKGROUND
  As important effector cells of the innate immune system, neutrophils are involved in rejection of solid organ and/or tissue transplants. But their role in rejection of oral mucosa transplantation (OMT) remains unclear. The aim of this study is to observe the spatial-temporal change of neutrophils during acute rejection of OMT.
  
  
  METHODS
  In a rat model of oral mucosal xenotransplantation, myeloperoxidase (MPO), an indicator of influx of neutrophils was detected by technique of ELISA on day 7 and 30 (D7, D30) of posttransplantation.
  
  
  RESULTS
  On D7, MPO level (6.183±0.416, x102 ng/mg) in the OMT group was significantly higher than in trauma (0.681±0.073, x102 ng/mg) and normal controls (0.262±0.043, x102 ng/mg) (P<0.001, respectively), and this level was found to correlate with the index of submandibular lymph nodes (ILN), an indicator of inflammation of rejection (r=0.909, P<0.05). Moreover, this level was decreased significantly under FK506 treatment (2.103±0.146, x102 ng/mg, P=0.005). On D30, MPO in the OMT group (1.063±0.096, x102 ng/mg) was lower significantly than that on D7 (P<0.001), although this level was still higher that of normal controls on D30 (0.532±0.112, x102 ng/mg, P=0.042).
  
  
  CONCLUSIONS
  Neutrophils infiltration was an early event of OMT, which may play important roles on acute rejection of OMT.
authors.affiliation: Guanghua School of Stomatology, Department of Pathology, Sun Yat-sen University, Cancer Center, Guangzhou, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Xiao-An
authors.initials: XA
authors.lastname: Tao
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
authors.forename: Juan
authors.initials: J
authors.lastname: Xia
authors.forename: Yulei
authors.initials: Y
authors.lastname: Huang
authors.forename: Bin
authors.initials: B
authors.lastname: Cheng
mesh_terms: D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009061:Mouth Mucosa / Q000637:transplantation*; D020556:Neutrophil Infiltration; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014183:Transplantation, Heterologous