title: Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication.
abstract: An up to 10,000-fold resistance against the biocontrol agent Cydia pomonella granulovirus (CpGV) was observed in field populations of codling moth, C. pomonella, in Europe. Following different experimental approaches, a modified peritrophic membrane, a modified midgut receptor, or a change of the innate immune response could be excluded as possible resistance mechanisms. When CpGV replication was traced by quantitative PCR in different tissues of susceptible and resistant insects after oral and intra-hemocoelic infection, no virus replication could be detected in any of the tissues of resistant insects, suggesting a systemic block prior to viral DNA replication. This conclusion was corroborated by fluorescence microscopy using a modified CpGV (bacCpGV(hsp-eGFP)) carrying enhanced green fluorescent gene (eGFP), which showed that infection in resistant insects did not spread. In conclusion, the different lines of evidence indicate that CpGV can enter but not replicate in the cells of resistant codling moth larvae.
authors.affiliation: Laboratory of Biotechnological Crop Protection, Department of Phytopathology, Agricultural Service Center Palatinate (DLR Rheinpfalz), Breitenweg 71, 67435 Neustadt a. d. Wstr., Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Asser-Kaiser
authors.forename: Pit
authors.initials: P
authors.lastname: Radtke
authors.forename: Said
authors.initials: S
authors.lastname: El-Salamouny
authors.forename: Doreen
authors.initials: D
authors.lastname: Winstanley
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Jehle
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000096:biosynthesis; D005060:Europe; D017930:Genes, Reporter; D025722:Granulovirus / Q000472:pathogenicity*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007915:Lepidoptera / Q000821:virology*; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins