title: [Growth characteristics of A549 lung adenocarcinoma multicellular spheroid].
abstract: BACKGROUND
  Owing to the limitation of monolayer tumour cells or transplanted tumor in radiation biology, multicellular spheroid (MTS) as a model of entity tumor had a wide range of application. This research aims to study the growth characteristics of A549 lung adenocarcinoma MTS so as to choose the best ones to perform experiments.
  
  
  METHODS
  1.75×10⁷ A549 cells in 35mL were transfered into the 100mm incubator sealed with 2% agarose to have a rotating culture with 60r/min. After 40 hours every MTS was transfered into each of the 96 wells of multi-well plates coated with 2% agarose to have a static culture.
  
  
  RESULTS
  During the rotation stage the MTS was round, the size was uniform, and the conjunction between cells was loose. After about 10 days of static culture, when the diameter of MTS at 429 to 570 μm the conjunction was close. After 26.5 days of static culture, the diameter of MTS became 1358.5 μm which could not be used for experiment. Observed under electron microscopy the cells in the MTS grew well without any apoptosis and necrosis. The cell cycle of MTS was dominated by G0 and G1 stage.
  
  
  CONCLUSIONS
  Combination of rotating and static culture is a simple method to produce MTS which has similar growth characteristics with entity tumor and is best suitable for experiment research when the diameter is about 500 μm.
authors.affiliation: Department of Radiology, Foshan Hospital of TCM, Guangzhou University of TCM, Foshan, Guangdong 528000, P.R.China.
authors.forename: Degang
authors.initials: D
authors.lastname: Shi
authors.forename: Gang
authors.initials: G
authors.lastname: Huang
authors.forename: Jisheng
authors.initials: J
authors.lastname: Miao
authors.forename: Xiangtong
authors.initials: X
authors.lastname: Lin
authors.forename: Xiaodong
authors.initials: X
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Su