title: Molecular identification of duck and quail common cytokine receptor γ chain genes.
abstract: Common cytokine receptor γ chain (γ(c)) family cytokines play crucial roles in the regulation of innate and adaptive immune responses. Unlike mammals, chickens possess two different γ(c) transcripts. To determine if this difference is present in other avian species, γ(c) cDNA and genomic clones from ducks and quails were investigated. Two different γ(c) transcripts were identified in both species and designated as duck γ(c)-a (duγ(c)-a), duγ(c)-b, quail γ(c)-a (quγ(c)-a), and quγ(c)-b. Comparisons between the duck and quail γ(c) cDNA and genomic sequences indicated that the two transcripts were produced by alternative splicing. Unexpectedly, the duγ(c)-b contained the fifth intron, a frame-switching 88-bp insertion, resulting in a receptor molecule lacking a transmembrane region. These findings indicate a possibility that avian species, unlike mammals, express two different γ(c) transcripts due to alternative splicing. This study is the first demonstration of an alternatively spliced γ(c) isoform that lacks a transmembrane domain.
authors.affiliation: College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-701, Republic of Korea.
authors.forename: Jipseol
authors.initials: J
authors.lastname: Jeong
authors.forename: Changhwan
authors.initials: C
authors.lastname: Lee
authors.forename: Jeongmi
authors.initials: J
authors.lastname: Yoo
authors.forename: Phil-Ok
authors.initials: PO
authors.lastname: Koh
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Hong H
authors.initials: HH
authors.lastname: Chang
authors.forename: Nong-Hoon
authors.initials: NH
authors.lastname: Choe
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003370:Coturnix / Q000235:genetics*; D004372:Ducks / Q000235:genetics*; D053631:Interleukin Receptor Common gamma Subunit / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary*
chemical_list: D053631:Interleukin Receptor Common gamma Subunit; D020033:Protein Isoforms