title: Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody clone #86.
abstract: A human-mouse hybridoma clone #86 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was newly established. To detect an antibody-binding sequence (epitope) on Ara h1, the monoclonal antibody #86 was reacted with multi-pin apparatus with a series of peptides synthesized from the amino acid sequence of Ara h1. The antibody #86 was found to bind to a peptide with amino acid sequence of 481EEEEDEDEEEEGSNREVRRY500. Further analysis with shorter pin-peptides with ten amino acid-long showed that the peptides reacted with the antibody #86 contained a sequence of 485DEDEEEE491. This might be an essential linear sequence of this epitope. When the 485DED487 part of the peptide was replaced by alanine, decreased binding of antibody #86 was observed.
authors.affiliation: Tamagawa University, Machida, Tokyo, Japan. shnmt920@agr.tamagawa.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
authors.forename: Mio
authors.initials: M
authors.lastname: Takeda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Naganawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Tomita
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takano-Ishikawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D052179:Antigens, Plant / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D010367:Arachis / Q000276:immunology; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D000939:Epitopes; D006023:Glycoproteins; D007075:Immunoglobulin M; D008565:Membrane Proteins; D010455:Peptides; D010940:Plant Proteins