title: A simple identification method of saliva by detecting Streptococcus salivarius using loop-mediated isothermal amplification.
abstract: We previously reported that detection of Streptococcus salivarius is feasible for proving the presence of saliva in a forensic sample. Here, a simple and rapid method for the detection of S. salivarius in forensic samples was developed that uses loop-mediated isothermal amplification (LAMP). The LAMP primer set was designed using S. salivarius-specific sequences of glucosyltransferase K. To simplify the procedure, the sample was prepared by boiling and mutanolysin treatment only, and the entire analytical process was completed within 2.5 h. The cut-off value was set at 0.1 absorbance units, measured at 660 nm, upon termination of the reaction. S. salivarius was identified in all saliva samples, but was not detected in other body fluids or on the skin surface. Using this method, S. salivarius was successfully detected in various mock forensic samples. We therefore suggest that this approach is useful for the identification of saliva in forensic practice.
authors.affiliation: Forensic Science Laboratory of Yamanashi Prefectural Police H.Q., Isawa, Fuefuki, Yamanashi, Japan. nakanishi@384.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohmori
authors.forename: Masaaki
authors.initials: M
authors.lastname: Hara
authors.forename: Aya
authors.initials: A
authors.lastname: Takada
authors.forename: Hideki
authors.initials: H
authors.lastname: Shojo
authors.forename: Noboru
authors.initials: N
authors.lastname: Adachi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D000818:Animals; D002415:Cats; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004285:Dogs; D005260:Female; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012463:Saliva / Q000382:microbiology*; D012661:Semen / Q000382:microbiology; D012867:Skin / Q000382:microbiology; D013291:Streptococcus / Q000235:genetics* / Q000302:isolation & purification*; D014556:Urine / Q000382:microbiology; D014621:Vagina / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial