title: Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for the detection of enteric pathogens.
abstract: A shortened DNA extraction protocol for the QIAsymphony SP was evaluated by quantitative and qualitative comparison of real-time PCR results of 150 co-extracted stool samples. The average ∆Cycle threshold value for positive pathogenic targets was 0.28 Ct. A consensus of 96.91%, with a correlation coefficient of 0.9880 was recorded.
authors.affiliation: Laboratory for Infectious Diseases, Department of Research and Development, Groningen, The Netherlands. e.van.zanten@infectielab.nl
authors.forename: E
authors.initials: E
authors.lastname: van Zanten
authors.forename: G J
authors.initials: GJ
authors.lastname: Wisselink
authors.forename: S
authors.initials: S
authors.lastname: Stoll
authors.forename: R
authors.initials: R
authors.lastname: Alvarez
authors.forename: A M D
authors.initials: AM
authors.lastname: Kooistra-Smid
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D013997:Time Factors
chemical_list: D004247:DNA