title: Mitochondrial and nuclear markers for the authentication of partridge meat and the specific identification of red-legged partridge meat products by polymerase chain reaction.
abstract: Two PCR assays for the identification of partridge meat (red-legged partridge, chukar partridge, barbary partridge, and gray partridge species) and the specific identification of red-legged partridge meat products were developed based on species-specific primers targeting the 12S ribosomal RNA mitochondrial gene. Moreover, various PCR techniques based on the use of random amplified polymorphic DNA markers and nuclear growth hormone and rhodopsin genes were tested to find a method for the differentiation between pure and hybrid red-legged partridges. Among these techniques, the PCR method based on the amplification and sequencing of a nuclear rhodopsin gene fragment was selected as a suitable tool for the discrimination among meats from pure and hybrid red-legged partridge individuals. The PCR assays reported in this work could be useful in inspection programs to verify the correct labeling of raw and heat-treated partridge meat products.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Rojas
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: M Á
authors.initials: MÁ
authors.lastname: Pavón
authors.forename: N
authors.initials: N
authors.lastname: Pegels
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
authors.forename: T
authors.initials: T
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D046094:Galliformes; D005819:Genetic Markers; D008460:Meat / Q000032:analysis*; D008928:Mitochondria / Q000737:chemistry*; D058977:Molecular Sequence Annotation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012335:RNA, Ribosomal; D013045:Species Specificity
chemical_list: D005819:Genetic Markers; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D004247:DNA