title: Ultrasound-targeted microbubble destruction mediated herpes simplex virus-thymidine kinase gene treats hepatoma in mice.
abstract: OBJECTIVE
  The purpose of the study was to explore the anti-tumor effect of ultrasound -targeted microbubble destruction mediated herpes simplex virus thymidine kinase (HSV-TK) suicide gene system on mice hepatoma.
  
  
  METHODS
  Forty mice were randomly divided into four groups after the models of subcutaneous transplantation tumors were established: (1) PBS; (2) HSV-TK (3) HSV-TK+ ultrasound (HSV-TK+US); (4) HSV-TK+ultrasound+microbubbles (HSV-TK+US+MB). The TK protein expression in liver cancer was detected by western-blot. Applying TUNEL staining detected tumor cell apoptosis. At last, the inhibition rates and survival time of the animals were compared among all groups.
  
  
  RESULTS
  The TK protein expression of HSV-TK+MB+US group in tumor-bearing mice tissues were significantly higher than those in other groups. The tumor inhibitory effect of ultrasound-targeted microbubble destruction mediated HSV-TK on mice transplantable tumor was significantly higher than those in other groups (p < 0.05), and can significantly improve the survival time of tumor-bearing mice.
  
  
  CONCLUSIONS
  Ultrasound-targeted microbubble destruction can effectively transfect HSV-TK gene into target tissues and play a significant inhibition effect on tumors, which provides a new strategy for gene therapy in liver cancer.
authors.affiliation: Department of Hepatobiliary Surgery, Second Affiliated Hospital of Chongqing Medical University, 76 Linjiang Road, Yuzhong District, Chongqing 400010, PR China.
authors.forename: Shiji
authors.initials: S
authors.lastname: Zhou
authors.forename: Shengwei
authors.initials: S
authors.lastname: Li
authors.forename: Zuojin
authors.initials: Z
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianping
authors.initials: J
authors.lastname: Gong
authors.forename: Changan
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000628:therapy*; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000628:therapy*; D051379:Mice; D045423:Microbubbles / Q000627:therapeutic use*; D018139:Simplexvirus / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014465:Ultrasonics / Q000379:methods*
chemical_list: D013937:Thymidine Kinase