title: Reduced expression of netrin-1 is associated with fetal growth restriction.
abstract: The objective of this study was to investigate the expression of netrin-1 in placenta from patients with fetal growth restriction (FGR) and its effect on the viability and apoptosis of human placental microvascular endothelial cells. Thirty-three patients with FGR (including eighteen severe cases) and twenty-four normal late pregnant women were investigated. The expression of netrin-1 in placental tissues was detected by employing immunohistochemistry, real-time PCR, and Western blotting. Human placental microvascular endothelial cells were isolated and, after treatment with netrin-1, examined for their viability and apoptosis by using MTT assay and flow cytometry. We demonstrated that the netrin-1 was present in placenta. Netrin-1 was significantly reduced in pregnant women with FGR as compared with the controls. Furthermore, netrin-1 enhanced the viability of human placental microvascular endothelial cells and inhibited their apoptosis. Netrin-1 may regulate the development of placental vessels and plays a key role in the pathogenesis of FGR.
authors.affiliation: Department of Gynecology and Obstetrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Qian-hua
authors.forename: Zhong
authors.initials: Z
authors.lastname: Shao-ping
authors.forename: Zhu
authors.initials: Z
authors.lastname: Jian-wen
authors.forename: Yang
authors.initials: Y
authors.lastname: Yun
authors.forename: Zou
authors.initials: Z
authors.lastname: Li
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005865:Gestational Age; D006801:Humans; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D000075388:Netrin-1; D010920:Placenta / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1