title: [Inhibitory effect of triptolide on proliferation of PC12 cells].
abstract: OBJECTIVE
  To investigate the effect of triptolide on proliferation of PC12 cells and the mechanism involved in the effect, and provide evidence for clinical use of triptolide in treatment of tumor.
  
  
  METHODS
  By means of morphological observation, MTT assay, flow cytometry (FCM) and RT-PCR, the effect of triptolide on the proliferation of PC12 cells was observed in vitro.
  
  
  RESULTS
  The proliferation inhibition was found on PC12 cells incubated with triptolide (5 x 10(3) or 25 x 10(3) g/L) for 24 h, 48 h and 72 h, and with the higher concentration of triptolide, the inhibition was stronger. Low concentration of triptolide (1 x 10(3) g/L) showed no significant effect on proliferation of PC12 cells. After treatment of PC12 cells with triptolide (5 x 10(3) g/L) for 24 h, increased percentage of G0-G1 phase and decreased percentage of S phase were found. Expression of translational elongation factor 2A3-2 was reduced after treatment of PC12 cells with triptolide (5 x 10(3) g/L). The expression of 2A3-2 was weaker in PC12 cells treated with triptolide for 48 h than for 24 h.
  
  
  CONCLUSIONS
  Triptolide inhibits the proliferation of PC12 cells. The inhibition may be realized by changing the expression of 2A3-2 and preventing the transition from G0-G1 phase to S phase.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Nantong University, Nantong, 226001, China.
authors.forename: Hui-Wei
authors.initials: HW
authors.lastname: Huang
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Qiu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D005434:Flow Cytometry; D016716:PC12 Cells / Q000187:drug effects; D010616:Phenanthrenes / Q000494:pharmacology*; D051381:Rats
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide