title: Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis.
abstract: A chimeric DNA vaccine co-expressing Eimeria tenella MZ5-7 and chicken IL-17 gene was constructed and its efficacy against E. tenella challenge was evaluated. The ORF of MZ5-7 from E. tenella's second generation merozoite and the mature interleukin 17 gene of chicken were cloned into the expression vector of pcDNA4.0 to construct DNA vaccine pcDNA4.0-MZ and pcDNA4.0-MZ-IL17. The expression of aim gene products in vivo was detected by western blotting. The expression of IL-2 and IFN-γ in chicken splenocytes was detected by RT-PCR 7 days post-immunization. The expression levels of the two cytokines in the pcDNA4.0-MZ-IL17 DNA vaccine immunized group were significantly higher than that in the pcDNA4.0-MZ immunized group (p<0.05). Either pcDNA4.0-MZ or pcDNA4.0-MZ-IL17 DNA vaccine could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA4.0-MZ-IL17 group was 190, which is higher than that of pcDNA4.0-MZ group and all the three control groups. In short, MZ5-7 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance the immunity DNA vaccine.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
authors.lastname: Geriletu
authors.forename: Lixin
authors.initials: L
authors.lastname: Xu
authors.lastname: Xurihua
authors.forename: Xiangrui
authors.initials: X
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000517:prevention & control*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D016786:Eimeria tenella / Q000145:classification*; D005786:Gene Expression Regulation / Q000276:immunology; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000469:parasitology / Q000517:prevention & control*; D015800:Protozoan Proteins; D016052:Protozoan Vaccines / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D016207:Cytokines; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA