title: Immunohistochemical detection of mutated epidermal growth factor receptors in pulmonary adenocarcinoma.
abstract: BACKGROUND
  Epidermal growth factor receptor (EGFR) mutational analysis has become essential in determining a therapeutic strategy for lung cancer. This study validated the reliability of recently generated rabbit monoclonal antibodies that recognise specific mutations of the EGFR protein.
  
  
  METHODS
  Pulmonary adenocarcinoma tissue from 20 previously genotyped specimens was prepared for immunohistochemical staining by two antibodies that recognise products of in-frame deletions in exon 19 (E746_A750del) and a point mutation that replaces leucine with arginine at codon 858 in exon 21 (L858R) of the EGFR gene.
  
  
  RESULTS
  The findings of EGFR-mutation-specific immunohistochemistry were concordant with the results of DNA analysis in 18 of 20 tested samples leading to 90% sensitivity and 100% specificity of the method.
  
  
  CONCLUSIONS
  This immunohistochemical assay for products of representative EGFR gene mutations may be performed reliably using tissue specimens from resected pulmonary adenocarcinomas.
authors.affiliation: Department of Chest Surgery, St Marianna University School of Medicine, Kawasaki, Kanagawa, Japan. h-nakamura@marianna-u.ac.jp
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Nakamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mochizuki
authors.forename: Takuo
authors.initials: T
authors.lastname: Shinmyo
authors.forename: Koji
authors.initials: K
authors.lastname: Ando
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kurimoto
authors.forename: Kumio
authors.initials: K
authors.lastname: Yokote
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics; D008297:Male; D009154:Mutation
chemical_list: D066246:ErbB Receptors