title: Synergistic antitumor effects of Escherichia coli maltose binding protein and Bacillus Calmette-Guerin in a mouse lung carcinoma model.
abstract: Maltose binding protein (MBP) is a component of the maltose transport system in the periplasm of Escherichia coli. It is commonly believed that MBP has minimal effects on the bioactivity, thus, it is widely used in the purification of recombinant proteins. Here, we found that the combined immunization with MBP and Bacillus Calmette-Guerin (BCG) significantly inhibited tumor growth compared with MBP or BCG immunization alone in a mouse lung carcinoma model. Further studies showed that MBP nonspecifically activated T helper 1 (Th1) cells and enhanced the BCG-induced Th1 cell activation. Moreover, MBP or BCG immunization alone increased the activities of natural killer (NK) cells and macrophages, and the combined immunization with MBP and BCG induced a synergistic effect on the activities of NK cells and macrophages. These results suggest that MBP possesses potent immune enhancement activities, and that the combination of MBP and BCG-induced synergistic antitumor effects might be mediated mainly through the activation of Th1 cells, NK cells and macrophages.
authors.affiliation: Department of Immunology, Norman Bethune College of Medicine, Jilin University, 126 Xinmin Street, Chaoyang District, 130021 Changchun, PR China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Weihua
authors.initials: W
authors.lastname: Ni
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhao
authors.forename: Fengli
authors.initials: F
authors.lastname: Wang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Gao
authors.forename: Guixiang
authors.initials: G
authors.lastname: Tai
mesh_terms: D000818:Animals; D001407:Bacillus / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000737:chemistry*; D005260:Female; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology / Q000628:therapy*; D058619:Maltose-Binding Proteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000276:immunology*
chemical_list: D058619:Maltose-Binding Proteins