title: Angiotensin I-converting enzyme inhibitory activity of gelatin hydrolysates and identification of bioactive peptides.
abstract: In this project we report on the angiotensin I-converting enzyme (ACE)-inhibitory activity of a bovine gelatin hydrolysate (Bh2) that was submitted to further hydrolysis by different enzymes. The thermolysin hydrolysate (Bh2t) showed the highest in vitro ACE inhibitory activity, and interestingly a marked in vivo blood pressure-lowering effect was demonstrated in spontaneously hypertensive rats (SHR). In contrast, Bh2 showed no effect in SHR, confirming the need for the extra thermolysin hydrolysis. Hence, an angiotensin I-evoked contractile response in isolated rat aortic rings was inhibited by Bh2t, but not by Bh2, suggesting ACE inhibition as the underlying antihypertensive mechanism for Bh2t. Using mass spectrometry, seven small peptides, AG, AGP, VGP, PY, QY, DY and IY or LY or HO-PY were identified in Bh2t. As these peptides showed ACE inhibitory activity and were more prominent in Bh2t than in Bh2, the current data provide evidence that these contribute to the antihypertensive effect of Bh2t.
authors.affiliation: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium.
authors.forename: Griet
authors.initials: G
authors.lastname: Herregods
authors.forename: John
authors.initials: J
authors.lastname: Van Camp
authors.forename: Nicole
authors.initials: N
authors.lastname: Morel
authors.forename: Bart
authors.initials: B
authors.lastname: Ghesquière
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Lieselot
authors.initials: L
authors.lastname: Vercruysse
authors.forename: Stephan
authors.initials: S
authors.lastname: Dierckx
authors.forename: Erwin
authors.initials: E
authors.lastname: Quanten
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000032:analysis* / Q000494:pharmacology*; D002417:Cattle; D004195:Disease Models, Animal; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D006973:Hypertension / Q000188:drug therapy / Q000201:enzymology; D008297:Male; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis* / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides; D005780:Gelatin; D007703:Peptidyl-Dipeptidase A