title: [Bacterial contamination on platelet concentrates: identification, antimicrobial susceptibility profile and transfusion-related sepsis].
abstract: BACKGROUND
  Bacterial sepsis associated with the transfusion of platelet concentrates (PCs) results in serious clinical implications for patients. Given these implications, certain procedures have been integrated into the preparation and quality control of blood components to reduce the risk of bacterial contamination. This article describes the prevalence of bacterial contamination on transfused PCs, the bacterial spectrum detected and their antimicrobial susceptibility profile and transfusion reactions in receptors.
  
  
  METHODS
  A total of 292 PCs (278 random and 14 per apheresis) from the Blood Center of the State of Rio Grande do Sul (HEMORGS), located in the city of Santa Maria, were tested. Quantities of 100 μL and 200 μL were collected from platelet bag tubing and seeded using two methodologies.
  
  
  RESULTS
  Using the qualitative methodology, bacteria were isolated in five units (1.7%; 5/292), while only one was isolated using the quantitative methodology. Staphylococcus epidermidis was the microorganism identified in all samples. Two patients died of transfusion-related sepsis.
  
  
  CONCLUSIONS
  Bacterial contamination due to PC transfusion is considered a major public health problem due to its association with high rates of morbidity and mortality. In this study only gram-positive microorganisms were isolated and none of the samples obtained by apheresis presented contamination.
authors.affiliation: Universidade Federal de Santa Maria, Santa Maria, RS.
authors.forename: Rosiéli
authors.initials: R
authors.lastname: Martini
authors.forename: Cláudia Barbisan
authors.initials: CB
authors.lastname: Kempfer
authors.forename: Mônica de Abreu
authors.initials: Mde A
authors.lastname: Rodrigues
authors.forename: Fábio Teixeira
authors.initials: FT
authors.lastname: Kuhn
authors.forename: Fabiane
authors.initials: F
authors.lastname: Rigatti
authors.forename: Viviane
authors.initials: V
authors.lastname: Ratzlaff
authors.forename: Zanoni
authors.initials: Z
authors.lastname: Segala
authors.forename: Rosmari
authors.initials: R
authors.lastname: Hörner
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001792:Blood Platelets / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017713:Platelet Transfusion / Q000009:adverse effects*; D018805:Sepsis / Q000209:etiology*; D013203:Staphylococcal Infections / Q000209:etiology*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents