title: Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay.
abstract: A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam-do 626-770, South Korea.
authors.forename: Go-Eun
authors.initials: GE
authors.lastname: Choi
authors.forename: Chulhun L
authors.initials: CL
authors.lastname: Chang
authors.forename: Jake
authors.initials: J
authors.lastname: Whang
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: O Jung
authors.initials: OJ
authors.lastname: Kwon
authors.forename: Won-Jung
authors.initials: WJ
authors.lastname: Koh
authors.forename: Sung Jae
authors.initials: SJ
authors.lastname: Shin
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D018598:Minisatellite Repeats; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity