title: Suppression of tumour metastasis in a murine osteosarcoma model with anti-CD25 monoclonal antibody treatment.
abstract: BACKGROUND
  Mouse regulatory T cells (Treg) may be deleted by intraperitoneal injection of anti-CD25 monoclonal antibody (mAb) (PC61). When Treg populations are thus suppressed, the immune system attacks tumours in autoimmune reactions.
  
  
  METHODS
  An osteosarcoma (LM8) was transplanted subcutaneously into C3H/He mice. Serial injections of PC61 were conducted from seven days before (pre-PC61 group) or from two days thereafter (post-PC61 group) and tumour growth and metastasis were examined four weeks later. A control group received PBS injections.
  
  
  RESULTS
  Subcutaneous tumours were reduced in size and the numbers of lung and liver metastatic colonies were significantly decreased in both pre- and post-PC61 groups compared to the control group.
  
  
  CONCLUSIONS
  Tumour suppression was effective even when injection of PC61 was performed two days after LM8 transplantation. These results indicate that such treatments might be suitable to be applied in the clinic after surgical operations.
authors.affiliation: Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan. ekozawae@gmail.com
authors.forename: Eiji
authors.initials: E
authors.lastname: Kozawa
authors.forename: Hideshi
authors.initials: H
authors.lastname: Sugiura
authors.forename: Junshi
authors.initials: J
authors.lastname: Wasa
authors.forename: Keishi
authors.initials: K
authors.lastname: Kohyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamada
authors.forename: Akiko
authors.initials: A
authors.lastname: Nishioka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Naoki
authors.initials: N
authors.lastname: Ishiguro
authors.forename: Osamu
authors.initials: O
authors.lastname: Taguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000556:secondary; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000188:drug therapy* / Q000276:immunology / Q000556:secondary
chemical_list: D000911:Antibodies, Monoclonal; C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit