title: Characterization and antiviral function of a cytosolic sensor gene, MDA5, in Japanese flounder, Paralichthys olivaceus.
abstract: Cytosolic pattern recognition receptors such as retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) play an important role in sensing viral RNAs. The receptor encoded by melanoma differentiation-associated gene 5 (MDA5), an RLR, recognizes viral RNA in the cytoplasm and enhances antiviral response in host cells. The full-length MDA5 gene in Japanese flounder, Paralichthys olivaceus was cloned and found to have 11,251 nucleotides. MDA5 transcript abundance was significantly increased in whole kidney infected with viral hemorrhagic septicemia virus (VHSV) as well as whole kidney and peripheral blood leukocytes stimulated with poly I:C in vitro. Hirame natural embryo (HINAE) cells overexpressing MDA5 showed a lower cytopathic effect (CPE) against VHSV, hirame rhabdovirus (HIRRV) and infectious pancreatic necrosis virus (IPNV) infection. When infected with VHSV, MDA5-overexpressing HINAE cells had 24-75 fold lower virus titer than normal HINAE cells. These results suggest that Japanese flounder MDA5 is involved in the induction of antiviral response.
authors.affiliation: Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-710, South Korea.
authors.forename: Maki
authors.initials: M
authors.lastname: Ohtani
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Jung
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D005432:Flounder / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D015870:Gene Expression; D029141:Novirhabdovirus; D009928:Organ Specificity; D010802:Phylogeny; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D018168:Receptors, Retinoic Acid