title: Deleterious effects of arsenic, benomyl and carbendazim on human endometrial cell proliferation in vitro.
abstract: OBJECTIVE
  We aimed to investigate the effects of arsenic (As), benomyl (Ben), and carbendazim (Carb) on endometrial cells.
  
  
  METHODS
  Human endometrial cells were obtained during diagnostic curettage. All cultured endometrial cells were divided into four groups: (1) 0 M (controls), (2) 10(-6) M, (3) 10(-5) M, (4) 10(-4) M for As, Ben and Carb. After 24 and 48 hours in culture, endometrial cell proliferations were assessed by diphenyltetrazolium bromide assay. The influences of different concentrations of As, Ben and Carb upon the endometrium were compared.
  
  
  RESULTS
  During the first 24 hours, As, Ben and Carb appeared to have insignificant influences upon endometrial growth. After 48 hours in culture, all three agents significantly inhibited endometrial growth. In As groups, cell absorption after 48 hours culture were 100% (group 1), 82.1% (group 2), 43.6% (group 3) and 35.3% (group 4). In Ben groups, cell absorption was 100% (1), 75.9% (2), 66.4% (3) and 49. 6% (4). In the Carb groups, cell absorption was 100% (1), 70.4% (2), 73.0% (3) and 76.7% (4).
  
  
  CONCLUSIONS
  The agents As, Ben and Carb appear to have inhibitory effects upon endometrial cells after 48 hours in culture.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taichung Taiwan.
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Chang
authors.forename: Yao-Yuan
authors.initials: YY
authors.lastname: Hsieh
authors.forename: Kung-Hao
authors.initials: KH
authors.lastname: Hsu
authors.forename: Horng-Der
authors.initials: HD
authors.lastname: Tsai
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Lin
authors.forename: Chih-Sheng
authors.initials: CS
authors.lastname: Lin
mesh_terms: D001151:Arsenic / Q000633:toxicity*; D001542:Benomyl / Q000633:toxicity*; D001562:Benzimidazoles / Q000633:toxicity*; D002219:Carbamates / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004717:Endometrium; D005260:Female; D006801:Humans
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; C006698:carbendazim; D001151:Arsenic; D001542:Benomyl