title: Transferrin-mediated targeting of bacteriophage HK97 nanoparticles into tumor cells.
abstract: OBJECTIVE
  Next-generation targeted nanodevices are currently under development for imaging and therapeutic applications. We engineered HK97 viral nanoparticles (VNPs) for tumor cell-specific targeting.
  
  
  METHODS
  A combination of genetic and chemical engineering methods were developed and applied to generate dual-labeled HK97 cysteine mutant particles displaying transferrin and fluorescent labels. The targeting properties of transferrin-conjugated VNPs were evaluated by in vitro experiments using different cancer cell lines.
  
  
  RESULTS
  We found that HK97-transferrin formulations were indeed targeted to cancer cells in vitro via the transferrin receptor. These studies highlight the utility and facilitate the further development of HK97-based VNPs.
authors.affiliation: Department of Molecular Biology & Center for Integrative Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Rick K
authors.initials: RK
authors.lastname: Huang
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Steinmetz
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Fu
authors.forename: Marianne
authors.initials: M
authors.lastname: Manchester
authors.forename: John E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy*; D011990:Receptors, Transferrin / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014168:Transferrin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D011990:Receptors, Transferrin; D013438:Sulfhydryl Compounds; D014168:Transferrin