title: RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities.
abstract: RING finger protein 152 (RNF152) is a novel RING finger protein and has not been well characterized. We report here that RNF152 is a canonical RING finger protein and has E3 ligase activity. It is polyubiqitinated partly through Lys-48-linked ubiquitin chains in vivo and this phenomenon is dependent on its RING finger domain and transmembrane domain. RNF152 is localized in lysosomes and co-localized with LAMP3, a lysosome marker. Moreover, over-expression of RNF152 in Hela cells induces apoptosis. These results suggest that RNF152 is a lysosome localized E3 ligase with pro-apoptotic activities. It is the first E3 ligase identified so far that is involved in lysosome-related apoptosis.
authors.affiliation: Key Laboratory of Infection and Immunity of Chinese Academy of Sciences, the Institute of Biophysics, Beijing, 100101, China.
authors.forename: Songling
authors.initials: S
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wu
authors.forename: Jiyan
authors.initials: J
authors.lastname: Zheng
authors.forename: Talin
authors.initials: T
authors.lastname: Suo
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D054829:RING Finger Domains; D016415:Sequence Alignment; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: C558222:RNF152 protein, human; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex