title: Uptake of CDDP-containing polymeric micelles by cells using particle induced X-ray emission.
abstract: Polymeric micelles loaded with cis-diamminedichloroplatinum(II), CDDP, (cisplatin micelles) enable higher accumulation in solid tumors and lower toxicities compared with CDDP alone. The combined use of cisplatin micelles with radiation is expected to enhance therapeutic effects and reduce side effects. The kinetics of cisplatin micelle uptake, however, have not been fully understood. Particle Induced X-Ray Emission has been employed in this study to measure the time transients of platinum in Chinese Hamster ovary cells. The results show that the platinum content of cells treated with cisplatin micelles increased more slowly than with CDDP alone, suggesting that cellular uptake could be controlled using micelles. The CDDP released from micelles was predominantly incorporated into the cells by diffusion. The uptake characteristics were further analyzed using micelles with different collapse rates. The results and techniques used in this study will be useful for designing an optimum treatment plan combining platinum-containing polymeric micelles and radiation in clinical applications.
authors.affiliation: Department of Nuclear Engineering and Management, School of Engineering, The University of Tokyo, Japan. mizuno@nuclear.jp
authors.forename: Kazue
authors.initials: K
authors.lastname: Mizuno
authors.forename: Teruaki
authors.initials: T
authors.lastname: Konishi
authors.forename: Masakazu
authors.initials: M
authors.lastname: Oikawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iso
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Imaseki
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Uesaka
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics*; D001692:Biological Transport; D016466:CHO Cells; D002945:Cisplatin / Q000493:pharmacokinetics*; D006224:Cricetinae; D003412:Cricetulus; D004058:Diffusion; D005260:Female; D007700:Kinetics; D008823:Micelles; D008954:Models, Biological; D010636:Phenols / Q000494:pharmacology*; D013997:Time Factors; D014965:X-Rays
chemical_list: D000970:Antineoplastic Agents; D008823:Micelles; D010636:Phenols; D002945:Cisplatin