title: Effect of in vitro exposure to benzo[a]pyrene, a component of cigarette smoke, on folliculogenesis, steroidogenesis and oocyte nuclear maturation.
abstract: We previoulsy quantified the concentration of benzo[a]pyrene (B[a]P) in the follicular fluid of women exposed to mainstream and/or sidestream cigarette smoke. The objective of this study was to quantify the effects of B[a]P-exposure, at concentrations representative of follicluar fluid concentrations, on folliculogenesis, on gonadal steroid and anti-müllerian hormone (AMH) output, oocyte growth, and nuclear maturation. Follicles (100-130 μm) isolated from ovaries of F1 hybrid (C57BL/6j×CBA/Ca) mice were cultured for 13 days in increasing concentrations of B[a]P (0 ng/ml (control) to 45 ng/ml). B[a]P treatment inhibited (p < 0 .05) antral follicle development, decreased estradiol output and follicle survival at the 45.0 ng/ml dose. B[a]P exposure decreased AMH output overall during preantral (p = 0.014) and antral (p = 0.026) follicle development but had no effect on progesterone output or oocyte growth and nuclear maturation in surviving follicles. These data suggest that B[a]P is an important toxic component of cigarette smoke that adversely affects follicular development and survival.
authors.affiliation: Department of Obstetrics and Gynecology, McMaster University, 1200 Main Street West, Hamilton, Ontario, L8N 3Z5, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Sadeu
authors.forename: W G
authors.initials: WG
authors.lastname: Foster
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000096:biosynthesis; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D016014:Linear Models; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009866:Oogenesis / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011374:Progesterone / Q000096:biosynthesis; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D012739:Gonadal Steroid Hormones; D012906:Smoke; D001564:Benzo(a)pyrene; D011374:Progesterone; D004958:Estradiol; D054304:Anti-Mullerian Hormone