title: [The protective effect of polysaccharide extracted from Laminaria japonica Aresch on vessels endothelial cell injury inducing by adrenaline].
abstract: OBJECTIVE
  To study the protective effect of Polysaccharide of Laminaria L01 on endothelial cell injury inducing by adrenaline.
  
  
  METHODS
  In order to observe the influence of L01 on the release of vWF in endothelial injured rats and HUVEC stimulated by adrenaline, a rat model of endothelial injury was established via injecting adrenaline, the damaged degree of vascular endothelial was evaluated by aortic immunity histochemistry, HUVEC was cultured in vitro, the content of vWF in rat plasma and in supernatant was measured by ELISA.
  
  
  RESULTS
  The measure of intact endodermis lengths (microm) stained by immunohistochemistry demonstrated the length in L01 high-dose group and low-dose group was obviously longer than that of model group (P < 0.05) in the 4th and 5th day during the model made. The content of vWF in rat plasma of L01 high-dose group was lower than that of model group (P < 0.05) in the 4th day, there were significant differences between this two groups, and the content of vWF in rat plasma of both L01 high-dose group and low-dose group was lower than that of model group (P < 0.05) in the 4th and 5th days. In the study of cultured HUVEC, on the 24 h, L01 groups (0.01 mg/ml and 0.1 mg/ml) decreased the supernatant vWF level, and on the 48 h, high-dose group (0.1 mg/ml) also decreased the supernatant vWF level, with significant difference compared with adrenaline group (10 microg/ml, P < 0.05).
  
  
  CONCLUSIONS
  L01 presented the protective effect on vascular endothelial cell.
authors.affiliation: Department of Physiology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xie
authors.forename: Ai-qun
authors.initials: AQ
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Meng-hua
authors.initials: MH
authors.lastname: Chen
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D004837:Epinephrine / Q000009:adverse effects; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D007795:Laminaria / Q000737:chemistry*; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014841:von Willebrand Factor; D004837:Epinephrine