title: Elevated dietary intake of Zn-methionate is associated with increased sperm DNA fragmentation in the boar.
abstract: Boars fed on ration of 200 ppm Zn methionate showed a significant increase (P < 0.001) in sperm DNA fragmentation when compared to animals fed on non-supplemented and rations containing 150 ppm Zn methionate. There was a positive correlation (R2 = 0.207; P = 0.002) between % sperm DNA fragmentation (SDF) and the concentration of Zn in spermatozoa. Increased Zn in the diet also resulted in a non-proportional increase in Zn concentration in the testis and spermatozoa but not in the epididymis; Zn in sperm accumulated at levels up to 50 times higher than that found in the seminal plasma and 10-13 times that found in the epididymis and testis, respectively. These results show that supplementation of dietary Zn at a concentration of 200 ppm had an adverse effect on boar sperm DNA quality and may be related to the ability of spermatozoa to accumulate Zn during spermiogenesis.
authors.affiliation: Policlinica Veterinaria y de Asesoría Zootécnica, Universidad Autónoma Metropolitana-Xochimilco, México D.F., Mexico.
authors.forename: Adelfa
authors.initials: A
authors.lastname: García-Contreras
authors.forename: Yasmin
authors.initials: Y
authors.lastname: De Loera
authors.forename: Carlos
authors.initials: C
authors.lastname: García-Artiga
authors.forename: Antonio
authors.initials: A
authors.lastname: Palomo
authors.forename: Jesús A
authors.initials: JA
authors.lastname: Guevara
authors.forename: José
authors.initials: J
authors.lastname: Herrera-Haro
authors.forename: Carmen
authors.initials: C
authors.lastname: López-Fernández
authors.forename: Steve
authors.initials: S
authors.lastname: Johnston
authors.forename: Jaime
authors.initials: J
authors.lastname: Gosálvez
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D053938:DNA Fragmentation / Q000187:drug effects*; D019587:Dietary Supplements / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016018:Least-Squares Analysis; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D009942:Organometallic Compounds / Q000378:metabolism / Q000633:toxicity*; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D034421:Sus scrofa; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D009942:Organometallic Compounds; C074508:zinc methionine; D008715:Methionine