title: Coupling of oligomerization and nucleotide binding in the AAA+ chaperone ClpB.
abstract: Members of the family of ATPases associated with various cellular activities (AAA+) typically form homohexameric ring complexes and are able to remodel their substrates, such as misfolded proteins or protein-protein complexes, in an ATP-driven process. The molecular mechanism by which ATP hydrolysis is coordinated within the multimeric complex and the energy is converted into molecular motions, however, is poorly understood. This is partly due to the fact that the oligomers formed by AAA+ proteins represent a highly complex system and analysis depends on simplification and prior knowledge. Here, we present nucleotide binding and oligomer assembly kinetics of the AAA+ protein ClpB, a molecular chaperone that is able to disaggregate protein aggregates in concert with the DnaK chaperone system. ClpB bears two AAA+ domains (NBD1 and NBD2) on one subunit and forms homohexameric ring complexes. In order to dissect individual mechanistic steps, we made use of a reconstituted system based on two individual constructs bearing either the N-terminal (NBD1) or the C-terminal AAA+ domain (NBD2). In contrast to the C-terminal construct, the N-terminal construct does not bind the fluorescent nucleotide MANT-dADP in isolation. However, sequential mixing experiments suggest that NBD1 obtains nucleotide binding competence when incorporated into an oligomeric complex. These findings support a model in which nucleotide binding to NBD1 is dependent on and regulated by trans-acting elements from neighboring subunits, either by direct interaction with the nucleotide or by stabilization of a nucleotide binding-competent state. In this way, they provide a basis for intersubunit communication within the functional ClpB complex.
authors.affiliation: Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
authors.forename: Nicolas D
authors.initials: ND
authors.lastname: Werbeck
authors.forename: Cathleen
authors.initials: C
authors.lastname: Zeymer
authors.forename: Julian N
authors.initials: JN
authors.lastname: Kellner
authors.forename: Jochen
authors.initials: J
authors.lastname: Reinstein
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D016963:Thermus thermophilus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D009711:Nucleotides; D000255:Adenosine Triphosphate