title: Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF.
abstract: We employed 19 recombinant antibodies (rAbs) generated from clonally expanded plasma cells, and native IgG from cerebrospinal fluid (CSF) of three multiple sclerosis (MS) patients for panning with phage displayed random peptide libraries. Specific peptide epitopes/mimotopes were identified and characterized. Importantly, peptide-antibody interactions were shared by rAbs and native IgG from the same patient. Three peptides strongly interacted with at least one other MS CSF, but not to inflammatory CNS controls. Database searches revealed several protein candidates including stress proteins, cell surface proteins, and neuronal proteins. Peptides derived from the candidate proteins were recognized by rAbs. Identification of peptide epitopes/mimotopes in MS may provide clues regarding disease-relevant antigens.
authors.affiliation: Department of Neurology, University of Colorado Denver at Anschutz Medical Campus, 12700 E. 19th Ave., Aurora, CO 80045, United States. Xiaoli.Yu@ucdenver.edu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yu
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
authors.forename: Laura
authors.initials: L
authors.lastname: Schambers
authors.forename: Olga
authors.initials: O
authors.lastname: Barmina
authors.forename: Mark
authors.initials: M
authors.lastname: Burgoon
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Bennett
authors.forename: Greg
authors.initials: G
authors.lastname: Owens
mesh_terms: D001323:Autoantibodies / Q000096:biosynthesis*; D002999:Clone Cells; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D019151:Peptide Library; D010950:Plasma Cells / Q000276:immunology* / Q000473:pathology; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D010446:Peptide Fragments; D019151:Peptide Library; D011994:Recombinant Proteins