title: Evaluation of p16INK4a immunohistochemical reactivity in endocervical glandular neoplasia.
abstract: OBJECTIVE
  To determine the expression of p16INK4a by immunohistochemistry in endocervical neoplasms.
  
  
  METHODS
  Forty-nine cases of endocervical glandular neoplasia were identified by record review. On histopathologic review of the original and new slides, 16 cases were identified as endocervical adenocarcinoma in situ (AIS), 31 were identified as invasive adenocarcinoma, and 2 had no remaining tumor identified. A representative paraffin block was selected from each case with tumor and sections cut from each block. One section from each case was stained with hematoxylin and eosin, 1 was processed for immunohistochemical study for p16INK4a, and 1 was a negative control. The nuclear immunoreactivity was graded positive, indeterminate, or negative.
  
  
  RESULTS
  Cases were classified AIS, adenocarcinoma, or no tumor. Of 16 cases of AIS, 15 (93.01%) cases were immunoreactive to p16INK4a; 1 case was negative. All cases of invasive adenocarcinoma, 31 of 31 cases (100%), were immunoreactive to p16INK4a.
  
  
  CONCLUSIONS
  p16INK4a reactivity can be used to help identify and assess in situ and invasive endocervical glandular neoplasms.
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32827-7480, USA. mamonroe@mail.ucf.edu
authors.forename: Mänette
authors.initials: M
authors.lastname: Monroe
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Wilkinson
authors.forename: Samantha L
authors.initials: SL
authors.lastname: Butler
mesh_terms: D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000473:pathology*; D010336:Pathology / Q000379:methods*; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16