title: Nisin-selectable food-grade secretion vector for Lactococcus lactis.
abstract: A nisin-resistant Lactococcus lactis strain TML01 was isolated from crude milk. A gene with 99% homology to the nisin-resistance gene, nsr, was identified. The food-grade secretion plasmid, pLEB690 (3746 bp), was constructed based on this novel nsr gene enabling primary selection with up to 5 μg nisin/ml. The functionality of pLEB690 as a secretion vector was shown by expressing and secreting the pediocin AcH gene papA in L. lactis. pLEB690 is therefore, a functional food-grade secretion vector potentially useful for the food industry.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Ruiqing
authors.initials: R
authors.lastname: Li
authors.forename: Timo M
authors.initials: TM
authors.lastname: Takala
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Qiao
authors.forename: Haijin
authors.initials: H
authors.lastname: Xu
authors.forename: Per E J
authors.initials: PE
authors.lastname: Saris
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D004351:Drug Resistance / Q000235:genetics*; D005516:Food Microbiology; D005822:Genetic Vectors / Q000235:genetics*; D013294:Lactococcus lactis / Q000187:drug effects* / Q000235:genetics*; D008892:Milk; D008969:Molecular Sequence Data; D009561:Nisin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin