title: Peptide separation through a CB[8]-mediated supramolecular trap-and-release process.
abstract: We demonstrate a supramolecular peptide separation approach by the selective immobilization of peptides bearing an N-terminal tryptophan onto a CB[8]-modified gold substrate, followed by electrochemical release. The CB[8]-stabilized heteroternary complexes were characterized by (1)H NMR, ESI-MS, UV/vis, and fluorescence spectroscopy and cyclic voltammetry. Micropatterned CB[8]-modified gold substrates were found to trap only the recognizable N-tryptophan-containing peptides from a peptide mixture that could be visualized as green peptide arrays under fluorescence microscopy. Subsequently, the bound peptides were released from the modified substrates by the controlled single-electron reduction of viologen. The fully reversible trap-and-release process was repeated for 13 cycles, and the cumulative release profile of the dye-peptide conjugate was monitored by fluorescence spectroscopy, indicating that no degradation occurred.
authors.affiliation: Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.forename: Monika
authors.initials: M
authors.lastname: Cziferszky
authors.forename: Dezhi
authors.initials: D
authors.lastname: Jiao
authors.forename: Karolina
authors.initials: K
authors.lastname: Wahlström
authors.forename: Jin
authors.initials: J
authors.lastname: Geng
authors.forename: Oren A
authors.initials: OA
authors.lastname: Scherman
mesh_terms: D004563:Electrochemistry; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D014364:Tryptophan / Q000737:chemistry
chemical_list: D010455:Peptides; D014364:Tryptophan