title: Identification of neonatal rat hippocampal radial glia cells in vitro.
abstract: The role of radial glia cells (RGCs) as neural progenitors and as guides for migrating neurons is well established, whereas their precise contribution to adult hippocampal neurogenesis remains less understood. To precisely study the properties of hippocampal RGCs under normal conditions in vitro, here we acquired the hippocampal RGCs of postnatal 1 d rats under adherent conditions in vitro, identified their astroglia and stem/progenitor properties. We found that the neonatal rat hippocampal RGCs had longer processes than the RGCs from fetal cerebral cortices, and these cells could be double-labeled by BLBP, GFAP, Vimentin with Nestin and expressed some stem/progenitor genes, these cells also presented multiple differentiation potentialities, albeit the ability of gliogenesis far exceeded the neurogenesis under normal culture conditions in vitro. Taken together, we acquired and identified some properties of the RGCs from neonatal rat hippocampi in vitro.
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong 226001, China.
authors.forename: Haoming
authors.initials: H
authors.lastname: Li
authors.forename: Guohua
authors.initials: G
authors.lastname: Jin
authors.forename: Jianbing
authors.initials: J
authors.lastname: Qin
authors.forename: Weiwei
authors.initials: W
authors.lastname: Yang
authors.forename: Meiling
authors.initials: M
authors.lastname: Tian
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Tan
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhang
authors.forename: Jinhong
authors.initials: J
authors.lastname: Shi
authors.forename: Linqing
authors.initials: L
authors.lastname: Zou
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology; D009457:Neuroglia / Q000145:classification* / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D014746:Vimentin; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases