title: [The regulative effects of polydatin on toll-like receptor 4 signal transduction pathway in lung ischemia/reperfusion injury in rabbits].
abstract: OBJECTIVE
  To investigate protective effects of polydatin(PD) during lung ischemia/reperfusion in rabbits and its potential mechanisms.
  
  
  METHODS
  Rabbit lung model of ischemia/reperfusion (I/R) injury was constituted in vivo. Thirty rabbits were divided into groups randomly: Control (C), I/R, PD group, respectively. Endotoxin (ET) in plasma was analyzed by End-point Chromogenic Assay, the expression of Toll-like receptor 4 (TLR4) mRNA, nuclear factor (NF)-kappaBp65 mRNA, intracellular adhesion molecule-1 (ICAM-1) mRNA were measured by RT-PCR, the morphological changes of lung tissue were observed with hematoxylin-eosin (HE) staining.
  
  
  RESULTS
  There was no significant difference in ET concentration of plasma between groups (all of P > 0.05). The expression of TLR-4 mRNA, NF-kappaBp65 mRNA and ICAM-1mRNA in I/R group were significantly increased as compared to C group and PD group, while those expressions in PD group were evidently higher than those in C group (all of P < 0.01). Light microscope showed that the lung pathological injuries in PD group were obviously alleviated as compared to I/R group.
  
  
  CONCLUSIONS
  PD might have a protective effect on lung ischemia/reperfusion injury by down-regulating TLR4 and NF-kappaB expression, then inhibiting the release of mediators of inflammation as ICAM-1.
authors.affiliation: Department of Pathophysioloy, Wenzhou Medical College, Wenzhou 325025, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Jin
authors.forename: Zheng-Jie
authors.initials: ZJ
authors.lastname: Xu
authors.forename: Wan-Tie
authors.initials: WT
authors.lastname: Wang
authors.forename: Yi-Xiao
authors.initials: YX
authors.lastname: Xu
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism / Q000503:physiopathology; D008168:Lung / Q000098:blood supply*; D008297:Male; D020011:Protective Agents / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism
chemical_list: D005960:Glucosides; D020011:Protective Agents; D012333:RNA, Messenger; D013267:Stilbenes; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA; D018799:Intercellular Adhesion Molecule-1; C058229:polydatin