title: [Fetal RHD genotyping by PCR using plasma from D negative pregnant women].
abstract: OBJECTIVE
  Free fetal DNA in maternal blood offers a non invasive method for prenatal diagnosis. The aim of this study is to perform fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
  
  
  METHODS
  Plasma sample from 120 D negative pregnant women from 10 to 40 gestational weeks were tested by conventional PCR for the presence of exons 7 and 10 of RHD gene. The results were compared with the RhD phenotype of the newborns.
  
  
  RESULTS
  In this study, the positive and the negative predictive value of the fetal RhD status were 98.7 and 96.7%, respectively.
  
  
  CONCLUSIONS
  These results demonstrate de feasibility of fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
authors.affiliation: Centre régional de transfusion sanguine, Madinat Al Irfane, BP 180, 10000 Rabat, Maroc. s.achargui@menara.ma
authors.forename: S
authors.initials: S
authors.lastname: Achargui
authors.forename: M
authors.initials: M
authors.lastname: Tijane
authors.forename: N
authors.initials: N
authors.lastname: Benchemsi
mesh_terms: D004247:DNA / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D005091:Exons / Q000235:genetics; D005240:Feasibility Studies; D005260:Female; D005331:Fetomaternal Transfusion / Q000097:blood*; D005333:Fetus / Q000276:immunology; D005838:Genotype; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D009018:Morocco; D010641:Phenotype; D010949:Plasma; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012203:Rh Isoimmunization / Q000097:blood* / Q000517:prevention & control; D012204:Rh-Hr Blood-Group System / Q000032:analysis / Q000235:genetics*
chemical_list: D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D004247:DNA