title: Individualized, targeted wound treatment based on the tissue bacterial level as a biological marker.
abstract: BACKGROUND
  The use of biologic markers to aid in individualizing wound treatment may help improve outcomes. A biologic marker that has been demonstrated to be predictive of healing in both chronic and acute wounds is wound tissue bacterial level. The objective of this study was to determine whether tissue bacterial level can be used to individualize wound treatment regimens with a stem-like cell-derived product.
  
  
  METHODS
  Amnion-derived cellular cytokine solution (ACCS) was topically applied to rat chronic wounds, and healing rates were measured.
  
  
  RESULTS
  Experimental wounds treated with ACCS demonstrated accelerated healing regardless of the tissue level of bacteria, compared with saline. As the level of tissue bacteria increased, the frequency of ACCS application required to obtain optimal results increased.
  
  
  CONCLUSIONS
  It appears that the biologic characteristic of tissue bacterial level can serve as a marker to predict the response of open granulating wounds treated with ACCS.
authors.affiliation: Institute of Tissue Regeneration, Repair, and Rehabilitation, Bay Pines VA Healthcare System, Bay Pines, FL, USA.
authors.forename: Yvonne N
authors.initials: YN
authors.lastname: Pierpont
authors.forename: M Georgina
authors.initials: MG
authors.lastname: Uberti
authors.forename: Francis
authors.initials: F
authors.lastname: Ko
authors.forename: Martin C
authors.initials: MC
authors.lastname: Robson
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Smith
authors.forename: Terry E
authors.initials: TE
authors.lastname: Wright
authors.forename: Wyatt G
authors.initials: WG
authors.lastname: Payne
mesh_terms: D000279:Administration, Cutaneous; D000650:Amnion; D000818:Animals; D058491:Bacterial Load; D015415:Biomarkers; D002056:Burns / Q000150:complications*; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000150:complications*; D005260:Female; D006801:Humans; D015993:Life Tables; D008297:Male; D039902:Multipotent Stem Cells; D011237:Predictive Value of Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D015163:Superinfection / Q000150:complications; D014945:Wound Healing; D014947:Wounds and Injuries / Q000209:etiology / Q000382:microbiology / Q000628:therapy*
chemical_list: D015415:Biomarkers; D016207:Cytokines