title: [DNA barcoding in medicinal plants Caprifoliaceae].
abstract: OBJECTIVE
  To determine the candidate sequences which can be used as DNA barcode to identify species in Caprifoliaceae family by screening out from four different DNA fragments sequences.
  
  
  METHODS
  PCR amplification, sequencing efficiency, differential intra- and interspecific divergences, the DNA barcoding gap and identification efficiency were used to evaluate these loci.
  
  
  RESULTS
  The ITS2 was used as a candidate sequence of DNA barcode to identify the species in Caprifoliaceae family, whose rate of success in identification in genera level was 100% and in species 96.6%, and psbA-trnH as a complementary barcode to ITS2 for Caprifoliaceae.
authors.affiliation: Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei College of Traditional Chinese Medicine, Wuhan 430065, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Keli
authors.initials: K
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Luo
authors.forename: Honglin
authors.initials: H
authors.lastname: Pan
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D027922:Caprifoliaceae / Q000235:genetics*; D004247:DNA / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis*; D001330:Electronic Data Processing / Q000379:methods; D007062:Identification, Psychological; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D004247:DNA