title: Detection of airborne trichothecene-producing Fusarium species in chicken houses.
abstract: One hundred and forty three airborne Fusarium isolates in chicken houses belonging to seven Fusarium species were analyzed by PCR with Tri5 gene as a specific marker of mycotoxin product . The result of Tri5 gene sequence analysis indicates that the PCR amplification products were 89%-96% identical to the previously reported Tri5 genes, which were all amplified from four F. poae isolates. T-2 toxin and DON was measured by immunoaffinity column and high performance liquid chromatography in Tri5-positive F. poae isolates after being cultured at constant and alternating temperatures. The production of T-2 toxin under alternating temperatures was 14 and 53 times higher than those at constant temperature of 8 °C and 25 °C. No DON was detected under either testing temperature condition. It is concluded that T-2 toxin-producing F. poae isolates were present in poultry houses, and the concentration of T-2 toxin produced by Tri5-positive F. poae isolates was increased under alternating temperatures. The application of Tri5-PCR associated with IMC-HPLC is an effective and accurate method for rapid detection of T-2 and DON mycotoxins.
authors.affiliation: College of Food Science Technology, Guangdong Ocean University, Zhanjiang, Guangdong Province, P R China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Wang
authors.forename: Tongjie
authors.initials: T
authors.lastname: Chai
authors.forename: Guozhong
authors.initials: G
authors.lastname: Lu
authors.forename: Lijun
authors.initials: L
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Sun
mesh_terms: D000391:Air Microbiology; D000818:Animals; D002681:China; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000235:genetics* / Q000378:metabolism*; D006799:Housing, Animal / Q000592:standards*; D011200:Poultry; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013605:T-2 Toxin / Q000378:metabolism; D014255:Trichothecenes / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D014255:Trichothecenes; D013605:T-2 Toxin