title: Results of HLA antibody testing using ELISA vs the fluorescent bead method and retrospective review of data for recipients of packed RBCs and platelets from male HLA-immunized donors.
abstract: We reviewed HLA antibody testing results using an enzyme-linked immunosorbent assay (ELISA) for all male blood donors at our institution during a 3.5-month period to look for HLA immunization. Confirmatory testing of 33 blood samples positive for HLA class I and/or II antibodies was performed using the fluorescent bead method. A retrospective review of recipients of packed RBCs and platelets processed from these 33 HLA-immunized male donors were conducted to identify transfusion-related acute lung injury and cognate antigens. The agreement rates between the methods for HLA class I and II antibodies were 21% (7/33) and 6% (2/33), respectively. We noted HLA antibodies in the male donors corresponding to cognate antigens in 2 recipients of packed RBCs and in 3 recipients of platelets. Of 8 donors positive for HLA antibodies, 5 did not have a history of blood transfusion. We conclude that ELISA was too sensitive and had a high false-positive rate for the detection of HLA class II antibodies.
authors.affiliation: Department of Laboratory Medicine, The University of Texas M.D. Anderson Cancer Center, Houston, 77054, USA.
authors.forename: Fleur M
authors.initials: FM
authors.lastname: Aung
authors.forename: Pedro
authors.initials: P
authors.lastname: Cano
authors.forename: Marcello
authors.initials: M
authors.lastname: Fernandez-Vina
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lichtiger
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D001782:Blood Donors; D001792:Blood Platelets / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017707:Erythrocyte Transfusion / Q000009:adverse effects; D005260:Female; D006680:HLA Antigens / Q000097:blood* / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017713:Platelet Transfusion / Q000009:adverse effects; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D006680:HLA Antigens