title: The association of ghrelin polymorphisms with coronary artery disease and ischemic chronic heart failure in an elderly Chinese population.
abstract: OBJECTIVE
  To investigate the association of coronary artery disease (CAD) and ischemic heart failure (IHF) with polymorphisms of the ghrelin gene in elderly Chinese patients.
  
  
  METHODS
  Fifty-six patients with ischemic heart failure, sixty patients with coronary artery disease without heart failure, and one hundred healthy control subjects participated in the study. The polymorphisms were evaluated by polymerase chain reaction, sequencing, and fragment length polymorphism analysis.
  
  
  RESULTS
  Only one single nucleotide polymorphism (SNP), Leu72Met (408C/A), was observed across all samples. Gene frequencies of CC and allele frequencies of C were significantly greater in the CAD with IHF group than those in the CAD without IHF group (p=0.025, p=0.011). There was no significant association between the Leu72Met SNP with coronary artery disease risk factors.
  
  
  CONCLUSIONS
  Our results suggest that a C allele at position 408 of the ghrelin gene is associated with genetic susceptibility to ischemic heart failure in Chinese elders.
authors.affiliation: Department of Geriatrics, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Huang
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Lv
authors.forename: Yun-Mei
authors.initials: YM
authors.lastname: Yang
mesh_terms: D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D003324:Coronary Artery Disease / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D054439:Ghrelin / Q000235:genetics*; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D054439:Ghrelin