title: [Effect of glucose-6-phosphate dehydrogenase on intracellular gsh level in Raji cells during oxidative stress].
abstract: OBJECTIVE
  To explore a role of G6PD in replenishment of intracellular GSH during oxidative stress.
  
  
  METHODS
  In vitro Raji cell was cultured, intracellular GSH levels and G6PD, GR, GPX activities were determined at different time points after PMS treatment when G6PD activity was inhibited or not by DHEA.
  
  
  RESULTS
  Intracellular GR, GPX, G6PD activities elevated significantly combined with GSH level decreased dramatically before 30 minutes, replenished gradually after 30 minutes and restore normal levels about 6 h after PMS treatment when G6PD was not inhibited. No change in GR and significant increase in GPX activity were shown following depleted GSH after PMS treatment when G6PD was inhibited by DHEA.
  
  
  CONCLUSIONS
  G6PD contributes to replenish intracellular GSH and is a critical factor regulating GSH levels during oxidative stress.
authors.affiliation: Union Hospital, Tong-ji Medical College, Hua-Zhong Science & Technology University,Wuhan 430022, China. Detaizhangwh@163.com
authors.forename: De-tai
authors.initials: DT
authors.lastname: Zhang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Hu
authors.forename: Yu-zhen
authors.initials: YZ
authors.lastname: Yang
mesh_terms: D045744:Cell Line, Tumor; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D018000:Receptors, Peptide / Q000378:metabolism
chemical_list: D018000:Receptors, Peptide; C035560:glutathione receptor; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005978:Glutathione