title: [Effect of vasonatrin peptide on the Ca2+ activated K+ channels of vascular smooth muscle cells isolated from rat mesentery arteries].
abstract: OBJECTIVE
  To investigate effect and mechanism of vasonatrin peptide (VNP) on Ca2+ activated K+ channels (K(Ca)) of vascular smooth muscle cells (VSMCs) isolated from rat mesentery arteries.
  
  
  METHODS
  Changes of K(Ca) induced by VNP were measured by the means of whole cell recording mode of patch clamp, furthermore effects of HS-142-1(0.3 g/L), 8-Br-cGMP and methylene blue (MB) were observed.
  
  
  RESULTS
  K(Ca) was significantly enhanced by VNP (10(-6) mol/L), which was mimicked by 8-Br-cGMP(10(-3) mol/L) and blocked completely by HS-142-1 or MB (2 x 10(-5) mol/L).
  
  
  CONCLUSIONS
  VNP increases K(Ca) of VSMCs isolated from rat mesenteric arteries, by binding with natriuretic peptide guanylate cyclase-coupled receptors and increasing the intracellular level of cGMP in VSMCs.
authors.affiliation: Experiment Teaching Center, Tangdu Hospital, Xi'an, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Miao-Zhang
authors.initials: MZ
authors.lastname: Zhu
authors.forename: Li-Bing
authors.initials: LB
authors.lastname: Liu
authors.forename: Bao-Ying
authors.initials: BY
authors.lastname: Chen
authors.forename: Shun-Yan
authors.initials: SY
authors.lastname: Lu
authors.forename: Jing-Jun
authors.initials: JJ
authors.lastname: Zhou
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Fu
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D008297:Male; D008638:Mesenteric Arteries / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology; D024681:Potassium Channels, Calcium-Activated / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024681:Potassium Channels, Calcium-Activated; C068986:ventricular natriuretic peptide, eel; D009320:Atrial Natriuretic Factor