title: Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus).
abstract: Manganese-superoxide dismutase (Mn-SOD) from Japanese flounder (Paralichthys olivaceus) hepatopancreas has been purified with high purification (781-fold) and recovery (10.8%). The molecular mass of the purified enzyme was estimated to be 26kDa by SDS-PAGE under reducing conditions. In activity staining by native-PAGE, the Japanese flounder Mn-SOD gave three active bands and exhibited KCN-insensitive activity. In addition, the electrophoretic mobility of this enzyme was observed to be faster than that of Japanese flounder Cu,Zn-SOD. On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. Analysis of nucleotide and deduced amino acid sequences revealed that the Mn-SOD cDNA consisted of a 64bp 5'-non-coding region, a 675bp open reading frame encoding 225 amino acids, and a 465bp 3'-non-coding region. The first 27 amino acids containing a mitochondria-targeting signal were highly conserved among other Mn-SODs.
authors.affiliation: Graduate School of Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Osatomi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Nagatomo
authors.forename: Asami
authors.initials: A
authors.lastname: Yoshida
authors.forename: Kenji
authors.initials: K
authors.lastname: Hara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D005432:Flounder / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D029941:Fish Proteins; D013482:Superoxide Dismutase