title: Transplantation of neural stem cells derived from human cord blood to the brain of adult and neonatal rats.
abstract: Searching for a reliable source of alternative neural stem cells for experimental treatment of neurological disorders we have established neural stem cell line derived from human umbilical cord blood (HUCB-NSC) (Buzanska et al. 2006). These cells have been shown to differentiate along neuronal and glial lineages in the promoting in vitro conditions. In the current study we transplanted HUCB-NSC into rat brain to determine whether the neural progenitors would be able to survive, migrate and eventually adopt neural phenotypes after exposure to central nervous system (CNS) microenvironment. Our experiments revealed that HUCB-NSC grafting into the brain of adult rats limited their survival up-to two weeks probably due to their elimination by severe immunological host reaction evoked by xenotransplantation. HUCB-NSC graft in neonates survived longer time in rat brain, migrated, proliferated and differentiated into neuronal cells however their presence in the host tissue did not exceed more than five weeks after transplantation.
authors.affiliation: NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jablonska
authors.forename: Hanna
authors.initials: H
authors.lastname: Kozlowska
authors.forename: Inga
authors.initials: I
authors.lastname: Markiewicz
authors.forename: Krystyna
authors.initials: K
authors.lastname: Domanska-Janik
authors.forename: Barbara
authors.initials: B
authors.lastname: Lukomska
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism; D001921:Brain / Q000502:physiology* / Q000601:surgery*; D049109:Cell Proliferation; D002552:Cerebral Ventricles / Q000601:surgery; D005312:Fetal Blood / Q000166:cytology; D005452:Fluoresceins; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology* / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D005452:Fluoresceins; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C069306:5-chloromethylfluorescein