title: Epigenetic modification of retinoic acid-treated human embryonic stem cells.
abstract: Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.
authors.affiliation: Department of Genetic Epidemiology, SNP Genetics, Inc., Seoul 153-803, Korea.
authors.forename: Hyun Sub
authors.initials: HS
authors.lastname: Cheong
authors.forename: Han Chul
authors.initials: HC
authors.lastname: Lee
authors.forename: Byung Lae
authors.initials: BL
authors.lastname: Park
authors.forename: Hyemin
authors.initials: H
authors.lastname: Kim
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Jang
authors.forename: Yong Mahn
authors.initials: YM
authors.lastname: Han
authors.forename: Seun-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Yong Sung
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyoung Doo
authors.initials: HD
authors.lastname: Shin
mesh_terms: D002454:Cell Differentiation; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin