title: PCR-based community structure studies of bacteria associated with eukaryotic organisms: a simple PCR strategy to avoid co-amplification of eukaryotic DNA.
abstract: PCR primers targeting conserved regions of the SSU rRNA gene are commonly used in bacterial community studies. For microbes associated with eukaryotes, co-amplification of eukaryotic DNA may preclude the analysis. We present a simple and efficient PCR strategy to obtain pure bacterial rDNA amplicons from samples predominated by eukaryotic DNA.
authors.affiliation: Norwegian University of Science and Technology, Department of Biotechnology, Trondheim, Norway. ingrid.bakke@biotech.ntnu.no
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bakke
authors.forename: Peter
authors.initials: P
authors.lastname: De Schryver
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
authors.forename: Olav
authors.initials: O
authors.lastname: Vadstein
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D056890:Eukaryota / Q000235:genetics* / Q000254:growth & development; D056186:Metagenomics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S