title: Analytical evaluation of the Tosoh HLC-723 G8 automated HPLC analyzer for hemoglobin analysis in beta-thalassemia mode.
abstract: OBJECTIVE
  The analytical performances of a new kit conceived for Hb variants separation and measurement procedures on an HPLC instrument (Tosoh HLC-723 G8) were studied.
  
  
  RESULTS
  Between-run and within-run precision tests were satisfactory for both HbA2 and HbF measurements. HbA2 and HbF values measured using the TOSOH HLC-723 G8 were correlated to those obtained using the Bio-Rad Variant II HPLC system (r=0.974 and 0.997 respectively) and to those given by the Sebia Capillary's system (r=0.980 and 0.996 respectively). Linearity was observed for HbA2 from 2.24% to 6.56% and for HbF from 0.5 to 6%.
  
  
  CONCLUSIONS
  The new analyzer Tosoh HLC-723 G8 was found to have a wide analytical range for both HbA2 and HbF. The G8 Mode beta-thal is suitable for a first-level laboratory screening for Hb analysis but also for a second-level test for the characterization of Hb variants after a first-line screening.
authors.affiliation: UMR 910, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Merono
authors.forename: Imane
authors.initials: I
authors.lastname: Agouti
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bonello-Palot
authors.forename: Chantal
authors.initials: C
authors.lastname: Paolasso
authors.forename: Nicolas
authors.initials: N
authors.lastname: Levy
authors.forename: Catherine
authors.initials: C
authors.lastname: Badens
mesh_terms: D001331:Automation; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D015203:Reproducibility of Results; D017086:beta-Thalassemia / Q000097:blood*
chemical_list: D006455:Hemoglobins, Abnormal