title: NPY promotes chemokinesis and neurogenesis in the rat subventricular zone.
abstract: The subventricular zone (SVZ) is a major reservoir for stem cells in the adult mammalian brain. Neural stem cells supply the olfactory bulb with new interneurons and provide cells that migrate towards lesioned brain areas. Neuropeptide Y (NPY), one of the most abundant neuropeptides in the brain, was previously shown to induce neuroproliferation on mice SVZ cells. In the present study, performed in rats, we demonstrate the endogenous synthesis of NPY by cells in the SVZ that suggests that NPY could act as an autocrine/paracrine factor within the SVZ area. We observed that NPY promotes SVZ cell proliferation as previously reported in mice, but does not affect self-renewal of SVZ stem cells. Additionally, this study provides the first direct evidence of a chemokinetic activity of NPY on SVZ cells. Using pharmacological approaches, we demonstrate that both the mitogenic and chemokinetic properties of NPY involve Y1 receptor-mediated activation of the ERK1/2 MAP kinase pathway. Altogether, our data establish that NPY through Y1 receptors activation controls chemokinetic activity and, as for mice, is a major neuroproliferative regulator of rat SVZ cells.
authors.affiliation: Institut de Physiologie et Biologie Cellulaires, University of Poitiers, CNRS, Poitiers Cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Thiriet
authors.forename: Fabienne
authors.initials: F
authors.lastname: Agasse
authors.forename: Camille
authors.initials: C
authors.lastname: Nicoleau
authors.forename: Christelle
authors.initials: C
authors.lastname: Guégan
authors.forename: François
authors.initials: F
authors.lastname: Vallette
authors.forename: Jean-Lud
authors.initials: JL
authors.lastname: Cadet
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jaber
authors.forename: João O
authors.initials: JO
authors.lastname: Malva
authors.forename: Valérie
authors.initials: V
authors.lastname: Coronas
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002552:Cerebral Ventricles / Q000166:cytology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009478:Neuropeptide Y / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism
chemical_list: C092926:BIBP 3226; D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; C066211:Npy1r protein, rat; D010446:Peptide Fragments; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide; C113547:neuropeptide Y (3-36); C062259:neuropeptide Y, Leu(31)-Pro(34)-; D001120:Arginine; D001973:Bromodeoxyuridine; D002118:Calcium