title: Antiviral activity of Alpinia katsumadai extracts against rotaviruses.
abstract: In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P[7] and porcine G5P[7] rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts [AK-1 (EtOH extract), AK-3 (H(2)O layer), AK-5 (40% methanol fraction), and AK-9-11 (H(2)O extract, polysaccharide fraction, supernatant fraction)] exhibited inhibitory activities against G5P[7] rotavirus with the EC(50) values ranging from 0.7±0.4 to 33.7±6.5 μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P[7] rotavirus, the with EC(50) values of 8.4±2.2 μg/mL, 6.5±0.8 μg/mL and 8.4±5.0 μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11 μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption.
authors.affiliation: Eco-Friendly Biomaterial Research Center and AI Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea.
authors.forename: Ha-Hyun
authors.initials: HH
authors.lastname: Kim
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Young Bae
authors.initials: YB
authors.lastname: Ryu
authors.forename: Jong Sun
authors.initials: JS
authors.lastname: Chang
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
authors.forename: Myra D T
authors.initials: MD
authors.lastname: Hosmillo
authors.forename: Mun-Chual
authors.initials: MC
authors.lastname: Rho
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Woo Song
authors.initials: WS
authors.lastname: Lee
mesh_terms: D032429:Alpinia; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000821:virology; D006385:Hemagglutination Inhibition Tests; D008253:Macaca mulatta; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000627:therapeutic use*; D012401:Rotavirus / Q000187:drug effects*; D012400:Rotavirus Infections / Q000188:drug therapy / Q000662:veterinary; D012639:Seeds; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy / Q000821:virology
chemical_list: D010936:Plant Extracts