title: [Effects of L-arginine and aminoguanidine on the endotoxin induced lung injury in rats].
abstract: OBJECTIVE
  To investigate the effects of L-arginine and nitric oxide synthase (NOS) inhibitor Aminoguanidine (AG) on endotoxin induced lung injury in rats.
  
  
  METHODS
  Forty eight healthy male SD rats weighing (300 +/- 20) g were used. The animals were anesthetized with 20% urethane 1 g x kg(-1). Common carotid artery (CAA) and common carotid vein (CAV) were exposed through a median incision in the neck. Mean arterial pressure (MAP) was measured through a pressure transducer connected with intubation of CAA. The animals were randomly divided into six groups: group 1: control: group 2: LPS (5 mg x kg(-1) intravenous injection, i.v.); group 3: AG (50 mg x kg(-1) intraperitoneal injection, IP); group 4: high dose L-arginine (500 mg x kg(-1), IP); group 5: low dose L-arginine (250 mg x kg(-1) IP). Group 6: L-arginine + AG (250 mg x kg(-1), 50 mg x kg(-1), IP). Group 1: The animals were killed 6 h after 0.9% saline solution was given. Group 2: 0.9% saline solution was given 3 h after LPS i.v. and the animals were killed 3 h after medication. Group 3, 4, 5 and 6: AG, L-arginine and L-arginine+ AG were given 3 h after LPS i.v. respectively and the animals were killed 3 h after medication respectively. The pulmonary was removed immediately. The pulmonary coefficient and water content in pulmonary tissue were calculated (%). The NO content in plasma, MDA content and NOS, SOD activity in the pulmonary tissue were measured.
  
  
  RESULTS
  L-arginine, AG and L-arginine + AG significantly decreased pulmonary coefficient and water content in pulmonary tissue and ameliorated endotoxin induced lung injury. AG and L-arginine + AG significantly decreased NO content in plasma, decreased MDA content and inhibited NOS activity and enhanced SOD activity in the pulmonary tissue.
  
  
  CONCLUSIONS
  It may be concluded that L-arginine, AG and L-arginine + AG have beneficial effects on lung injury induced by LPS.
authors.affiliation: Hebei Academy of Medical Sciences, Hebei Shijiazhuang 050021, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Li
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: Dong
authors.forename: Lan-Fang
authors.initials: LF
authors.lastname: Li
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Xie
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000627:therapeutic use*; D004731:Endotoxins / Q000009:adverse effects; D006146:Guanidines / Q000008:administration & dosage / Q000627:therapeutic use*; D055370:Lung Injury / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004731:Endotoxins; D006146:Guanidines; D009569:Nitric Oxide; D008315:Malondialdehyde; D001120:Arginine; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; C004479:pimagedine