title: Keratinocytes play a role in the immunity to Herpes simplex virus type 2 infection.
abstract: Herpes simplex virus type 2 (HSV-2) infection is the most common cause of genital ulcerative disease in the developed world. Keratinocytes are the primary cells involved in clinical lesions caused by HSV-2. In our study, we intensively examined cytokine expression in the HSV-2-infected keratinocytes. We observed upregulation of a series of cytokines including early-induced antiviral cytokines as interferons α, β (IFN-α, β), tumor necrosis factor α (TNF-α), colony stimulating factors (CSFs) as G-CSF, GM-CSF, interleukin 3 (IL-3), growth factors (EGF, KGF, and IGF-β1), defensins, selectins, leukocyte function-associated antigens (LFAs,) and toll-like receptors (TLR-2, 3, 4, and 9). More importantly, we found that HSV-2-infected keratinocytes stimulated the proliferation of lymphocytes in co-cultivation system. These data suggest that keratinocytes participate in the immune response to HSV-2 infection in two ways. They secrete inflammatory cytokines to resist the HSV-2 infection directly and recruit the immune cells to eliminate the primary infection indirectly and enhance the adaptive immunity to prevent subsequent infections.
authors.affiliation: Shenzhen Key Lab for Translational Medicine of Dermatology, Guangdong Province, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shao
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: Ch
authors.initials: Ch
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhong
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Wan
authors.forename: B
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D015603:Keratinocytes / Q000276:immunology* / Q000821:virology; D015854:Up-Regulation; D014709:Vero Cells
chemical_list: D016207:Cytokines