title: [Two different techniques for volume blood reduction obtained from umbilical cord].
abstract: BACKGROUND
  To preserve the umbilical cord blood (UCB), the volume containing the cells must be reduced before freezing, but the quality, quantity and functionality of the cells has to be preserved during this procedure. The aim is to compare the performance of two different techniques for volume reduction.
  
  
  METHODS
  A semiautomatic system and an automatic system were compared as two different UCB volume reduction techniques. Total nucleated cell (TNC) counts and viability were measured before and after volume reduction. The CD34+ cell counts also were measured.
  
  
  RESULTS
  Seventy units of cord blood cells (UCB) were collected. Thirty-three volume reduction procedures were performed by semiautomatic system and thirty-seven by automatic system. The volume recovered and the CD34+ count in both techniques was similar, although the viability differed slightly (1% higher by Optipress II).
  
  
  CONCLUSIONS
  The semiautomatic and automatic techniques are suitable to reduce volumes of UCB units.
authors.affiliation: Banco Central de Sangre, Centro Médico Nacional La Raza, Instituto Mexicano del Seguro Social, Distrito Federal, México. gamardoc@yahoo.com.mx
authors.forename: Gamaliel
authors.initials: G
authors.lastname: Benítez-Arvizu
authors.forename: Samantha
authors.initials: S
authors.lastname: Martínez
authors.forename: Ángel
authors.initials: Á
authors.lastname: Guerra
authors.forename: Fernando
authors.initials: F
authors.lastname: Luna
authors.forename: Yanin
authors.initials: Y
authors.lastname: Romero
authors.forename: Bárbara Alicia
authors.initials: BA
authors.lastname: Novelo-Garza
mesh_terms: D001773:Blood Cells; D001793:Blood Preservation / Q000379:methods*; D005312:Fetal Blood; D006801:Humans