title: Immune responses of mice to prime-boost vaccination with the recombinant DNA and Fowlpox virus both expressing HIV-2 Gag-gp105.
abstract: Human immunodeficiency viruses 1 and 2 (HIV-1, 2) present a public health problem for which there is neither an effective antiviral therapy nor a preventive vaccine. In this study, the immune responses of mice to prime-boost vaccination with the recombinant DNA (rDNA) and recombinant Fowlpox virus (rFPV) both expressing HIV-2 Gag-gp105 chimeric protein, were compared to those elicited by each vector alone. Mice primed with the rDNA and boosted with the rFPV showed HIV-2-specific antibody levels, splenic CD4+ and CD8+ T-lymphocyte numbers, and Gag-gp105-specific cytotoxic T-lymphocytes (CTL) activity increased by 20-30% as compared with those elicited by these vaccines alone. These findings suggested that the prime-boost strategy combining rDNA and rFPV elicited significant Gag-gp105 - specific cellular and humoral immune responses, thus supporting this novel approach to the immunization against HIV infections.
authors.affiliation: Tianjin University, Tianjin, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Song
authors.forename: L S
authors.initials: LS
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Jin
authors.forename: Ch
authors.initials: Ch
authors.lastname: Li
authors.forename: N
authors.initials: N
authors.lastname: Jin
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002642:Chick Embryo; D004195:Disease Models, Animal; D005260:Female; D005587:Fowlpox virus / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D015498:HIV-2 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000821:virology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D019444:Vaccines, DNA; D054299:env Gene Products, Human Immunodeficiency Virus; C069790:gp105 envelope protein, Human immunodeficiency virus 2