title: [Oxacillin-resistant coagulase-negative Staphylococci bacteremia at a teaching hospital in Santa Maria, State of Rio Grande do Sul, Brazil].
abstract: BACKGROUND
  This study aimed to characterize the prevalence and susceptibility profile to oxacillin-resistant Coagulase-negative Staphylococci strains isolated from blood cultures in a teaching hospital, located in Santa Maria, RS. In addition, different methodologies for phenotypic characterization of mecA-mediated oxacillin resistance were compared with genotypic reference testing.
  
  
  METHODS
  After identification (MicroScan - Siemens), the isolates were tested for antimicrobial sensitivity using disk diffusion and automation (MicroScan - Siemens). The presence of mecA gene was identified by the polymerase chain reaction molecular technique.
  
  
  RESULTS
  The most common species was Staphylococcus epidermidis (n=40, 67%). The mecA gene was detected in 54 (90%) strains, while analysis of the sensitivity profiles revealed a high rate of resistance to multiple classes of antimicrobial drugs. However, all isolates were uniformly sensitive to vancomycin and tigecycline. The cefoxitin disk was the phenotypic method that best correlated with the gold standard.
  
  
  CONCLUSIONS
  Analysis of the clinical significance of CoNS isolated from hemocultures and the precise detection of oxacillin resistance represent decisive factors for the correct choice of antibiotic therapy. Although vancomycin constitutes the normal treatment in most Brazilian hospitals, reduction in its use is recommended.
authors.affiliation: Universidade Federal de Santa Maria, Santa Maria, RS.
authors.forename: Fabiane
authors.initials: F
authors.lastname: Rigatti
authors.forename: Maísa Kraulich
authors.initials: MK
authors.lastname: Tizotti
authors.forename: Rosmari
authors.initials: R
authors.lastname: Hörner
authors.forename: Vanessa Oliveira
authors.initials: VO
authors.lastname: Domingues
authors.forename: Rosiéli
authors.initials: R
authors.lastname: Martini
authors.forename: Letícia Eichstaedt
authors.initials: LE
authors.lastname: Mayer
authors.forename: Fábio Teixeira
authors.initials: FT
authors.lastname: Khun
authors.forename: Chirles Araújo
authors.initials: CA
authors.lastname: de França
authors.forename: Mateus Matiuzzi
authors.initials: MM
authors.lastname: da Costa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics*; D003030:Coagulase / Q000235:genetics*; D052978:Disk Diffusion Antimicrobial Tests; D005838:Genotype; D006784:Hospitals, Teaching; D006801:Humans; D010403:Penicillin Resistance / Q000187:drug effects / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus