title: Nitric oxide changes distinct aspects of the glycophenotype of human neuroblastoma NB69 cells.
abstract: It is an open question whether the presence of nitric oxide (NO) affects the cell glycophenotype. A panel of six plant lectins was used in this study to monitor distinct aspects of cell surface glycosylation under nitrosative stress. We determined that treating human neuroblastoma NB69 cells with the long-lived NO donor 2,2'-(hydroxynitrosohydrazono)bis-ethanimine (DETA/NO) and monitoring the non-apoptotic adherent cell population significantly increases the presentation of N-glycans as detected by concanavalin A. Examining fine-structural features, bisected N-glycans and branch-end tailoring including α2,6-sialylation were found to be enhanced. Confocal fluorescence microscopy and cell permeabilization experiments pointed to a major effect of NO on the extent of cell surface N-glycan presentation. We also show that NO increases the level of protein O-GlcNAcylation, a multifunctional post-translational modification. Our results thus establish the first evidence for NO as modulator of distinct aspects of cell glycosylation.
authors.affiliation: Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Arturo Duperier 4, E-28029 Madrid, Spain. Marlies.VandeWouwer@pharm.kuleuven.be
authors.forename: Marlies
authors.initials: M
authors.lastname: Van de Wouwer
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Hans-J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Antonio
authors.initials: A
authors.lastname: Villalobo
mesh_terms: D045744:Cell Line, Tumor / Q000187:drug effects; D003671:DEET / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D018613:Microscopy, Confocal; D009447:Neuroblastoma / Q000503:physiopathology; D009569:Nitric Oxide / Q000494:pharmacology*; D010641:Phenotype; D037121:Plant Lectins / Q000737:chemistry
chemical_list: D016166:Free Radical Scavengers; D037121:Plant Lectins; D003671:DEET; D009569:Nitric Oxide