title: Cryo-electron tomography: methodology, developments and biological applications.
abstract: Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.
authors.affiliation: Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Vanhecke
authors.forename: S
authors.initials: S
authors.lastname: Asano
authors.forename: Z
authors.initials: Z
authors.lastname: Kochovski
authors.forename: R
authors.initials: R
authors.lastname: Fernandez-Busnadiego
authors.forename: N
authors.initials: N
authors.lastname: Schrod
authors.forename: W
authors.initials: W
authors.lastname: Baumeister
authors.forename: V
authors.initials: V
authors.lastname: Lučić
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D055032:Electron Microscope Tomography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods