title: Perilla frutescens seed agar, a new medium for identification of the Cryptococcus species complex: evaluation for all major molecular types.
abstract: Here we present a new and simple medium made by Perilla frutescens seed as a tool for identification of the Cryptococcus species complex, namely Cryptococcus neoformans and Cryptococcus gattii. Its usefulness was evaluated for all major molecular types within the Cryptococcus species complex. As a result, this medium is better for identification of the species complex compared with Guizotia abyssinica or Helianthus annuus seed medium.
authors.affiliation: Medical Mycology Laboratory, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Feng
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Yao
authors.forename: Bo
authors.initials: B
authors.lastname: Ling
authors.forename: Daming
authors.initials: D
authors.lastname: Ren
authors.forename: Wanqing
authors.initials: W
authors.lastname: Liao
mesh_terms: D000362:Agar; D056285:Cryptococcus gattii / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D009172:Mycology / Q000379:methods*; D031344:Perilla frutescens / Q000737:chemistry; D010860:Pigments, Biological / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D003470:Culture Media; D010860:Pigments, Biological; D010936:Plant Extracts; D000362:Agar