title: Quencher-free molecular beacon: Enhancement of the signal-to-background ratio with graphene oxide.
abstract: We report the highly improved version of quencher-free molecular beacon (QF-MB) system by using graphene oxide (GO) as an external quencher. This QF-MB/GO system provided a higher S/B ratio (31.0) relative to that (2.2) of the same system in the absence of GO, while retaining a high selectivity for fully matched over single-base-mismatched targets.
authors.affiliation: Laboratory for Modified Nucleic Acid Systems, Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: Jeong Wu
authors.initials: JW
authors.lastname: Yi
authors.forename: Jaesung
authors.initials: J
authors.lastname: Park
authors.forename: N Jiten
authors.initials: NJ
authors.lastname: Singh
authors.forename: Il Joon
authors.initials: IJ
authors.lastname: Lee
authors.forename: Kwang S
authors.initials: KS
authors.lastname: Kim
authors.forename: Byeang Hyean
authors.initials: BH
authors.lastname: Kim
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D008958:Models, Molecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D010087:Oxides; D006108:Graphite