title: Effect of intracoronary infusion of bone marrow mononuclear cells or peripheral endothelial progenitor cells on myocardial ischemia-reperfusion injury in mini-swine.
abstract: OBJECTIVE
  To simulate and assess the clinical effect of intracoronary infusion of bone marrow mononuclear cells or peripheral endothelial progenitor cells on myocardial reperfusion injury in mini-swine model.
  
  
  METHODS
  Twenty-three mini-swine with myocardial reperfusion injury were used as designed in the study protocol. About (3.54 +/- 0.90) x 10(8) bone marrow mononuclear cells (MNC group, n = 9) or (1.16 +/- 1.07) x 10(7) endothelial progenitor cells (EPC group, n = 7) was infused into the affected coronary segment of the swine. The other mini-swine were infused with phosphate buffered saline as control (n = 7). Echocardiography and hemodynamic studies were performed before and 4 weeks after cell infusion. Myocardium infarction size was calculated. Stem cell differentiation was analyzed under a transmission electromicroscope.
  
  
  RESULTS
  Left ventricular ejection fraction dropped by 0% in EPC group, 2% in MNC group, and 10% in the control group 4 weeks after cell infusion, respectively (P < 0.05). The systolic parameters increased in MNC and EPC groups but decreased in the control group. However, the diastolic parameters demonstrated no significant change in the three groups (P > 0.05). EPC decreased total infarction size more than MNC did (1.60 +/- 0.26 cm2 vs. 3.71 +/- 1.38 cm2, P < 0.05). Undermature endothelial cells and myocytes were found under transmission electromicroscope.
  
  
  CONCLUSIONS
  Transplantation of either MNC or EPC may be beneficial to cardiac systolic function, but might not has obvious effect on diastolic function. Intracoronary infusion of EPC might be better than MNC in controlling infarction size. Both MNC and EPC may stimulate angiogenesis, inhibit fibrogenesis, and differentiate into myocardial cells.
authors.affiliation: Center of Coronary Heart Disease, Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China.
authors.forename: Chong-Jian
authors.initials: CJ
authors.lastname: Li
authors.forename: Run-Lin
authors.initials: RL
authors.lastname: Gaol
authors.forename: Yue-Jin
authors.initials: YJ
authors.lastname: Yang
authors.forename: Feng-Huan
authors.initials: FH
authors.lastname: Hu
authors.forename: Wei-Xian
authors.initials: WX
authors.lastname: Yang
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: You
authors.forename: Lai-Feng
authors.initials: LF
authors.lastname: Song
authors.forename: Ying-Mao
authors.initials: YM
authors.lastname: Ruan
authors.forename: Shu-Bin
authors.initials: SB
authors.lastname: Qiao
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Chen
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D042783:Endothelial Cells / Q000166:cytology*; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D013234:Stem Cells / Q000166:cytology*; D013552:Swine; D013556:Swine, Miniature