title: Avian CD154 enhances humoral and cellular immune responses induced by an adenovirus vector-based vaccine in chickens.
abstract: Recombinant adenoviral vectors have emerged as an attractive system for veterinary vaccines development. However, for poultry vaccination a very important criterion for an ideal vaccine is its low cost. The objective of this study was to test the ability of chicken CD154 to enhance the immunogenicity of an adenoviral vector-based vaccine against avian influenza virus in order to reduce the amount of antigen required to induce an effective immune response in avian. Chickens were vaccinated with three different doses of adenoviral vectors encoding either HA (AdHA), or HA fused to extracellular domain chicken's CD154 (AdHACD). Hemagglutination inhibition (HI) assay and relative quantification of IFN-γ showed that the adenoviral vector encoding for the chimeric antigen is able to elicit an improved humoral and cellular immune response, which demonstrated that CD154 can be used as a molecular adjuvant allowing to reduce in about 50-fold the amount of adenoviral vector vaccine required to induce an effective immune response.
authors.affiliation: Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Chile. osanchez@udec.cl
authors.forename: Oliberto
authors.initials: O
authors.lastname: Sánchez Ramos
authors.forename: Alain
authors.initials: A
authors.lastname: González Pose
authors.forename: Silvia
authors.initials: S
authors.lastname: Gómez-Puerta
authors.forename: Julia
authors.initials: J
authors.lastname: Noda Gomez
authors.forename: Armando
authors.initials: A
authors.lastname: Vega Redondo
authors.forename: Julio César
authors.initials: JC
authors.lastname: Águila Benites
authors.forename: Lester
authors.initials: L
authors.lastname: Suárez Amarán
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Parra
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Toledo Alonso
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D023201:CD40 Ligand / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002645:Chickens; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D007111:Immunity, Cellular / Q000276:immunology*; D056724:Immunity, Humoral / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D014765:Viral Vaccines; D023201:CD40 Ligand; D007371:Interferon-gamma