title: Optimization of Spongiochloris sp. biomass production in the abattoir digestate.
abstract: In the present study, the abattoir digestate was used as a culture medium for Spongiochloris sp. growth with added mineral components under optimized conditions in batch culture. Firstly, an Hadamard matrix was used to investigate the impact of certain influencing factors on the Spongiochloris sp. growth. Then, a fractional factorial design 2(7-4) was successfully employed to optimize the concentration of different added components to abattoir digestate for increased Spongiochloris sp. biomass production. The major influencing factors were NaHCO(3) and FeSO(4) at a level of 2000 mg/L and 5mg/L, respectively. A high biomass production of 5.29 × 10(6) cell/mL and an important content of chlorophyll a of 65.32 mg/L were obtained after 42 days of culture of Spongiochloris sp. on the defined abattoir medium at static conditions.
authors.affiliation: Laboratory of Microbial Ecology and Technology, Department of Biological and Chemical Engineering, National Institute of Applied Sciences and Technology, B. P 676, 1080 Tunis, and University of 7th November at Carthage, Tunis, Tunisia.
authors.forename: Faten Saïdane
authors.initials: FS
authors.lastname: Bchir
authors.forename: Hana
authors.initials: H
authors.lastname: Gannoun
authors.forename: Soumaya
authors.initials: S
authors.lastname: El Herry
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
mesh_terms: D000003:Abattoirs; D000818:Animals; D001673:Biodegradation, Environmental; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002734:Chlorophyll / Q000737:chemistry; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000378:metabolism*; D003470:Culture Media; D007220:Industrial Waste; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D015233:Models, Statistical; D012722:Sewage; D013431:Sulfates / Q000737:chemistry
chemical_list: D003470:Culture Media; D007220:Industrial Waste; D012722:Sewage; D013431:Sulfates; D002734:Chlorophyll; D007501:Iron; D000077194:Chlorophyll A