title: IMAPlate based miniature, high sensitive, rapid screening method for detecting bioengineered, secreted lipase activities in yeast expression systems.
abstract: A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.
authors.affiliation: Hochschule für Life Sciences, Fachhochschule Nordwestschweiz Grandenstrasse 40, Muttenz.
authors.forename: Michel A
authors.initials: MA
authors.lastname: Sciotti
authors.forename: Guo Jun
authors.initials: GJ
authors.lastname: Chen
authors.forename: Daniel
authors.initials: D
authors.lastname: Gygax
mesh_terms: D005818:Genetic Engineering; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008904:Miniaturization; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D008049:Lipase