title: An epidermis-specific chitin synthase CDNA in Choristoneura fumiferana: cloning, characterization, developmental and hormonal-regulated expression.
abstract: Chitin synthase catalyzes chitin synthesis in the exoskeleton, tracheal system and gut during insect development. A chitin synthase 1 (CfCHS1) cDNA was identified and cloned from the spruce budworm, Choristoneura fumiferana. The CfCHS1 cDNA is 5,300 bp in length and codes a 1,564-amino acid protein with a molecular mass of 178 kDa. The deduced protein contains 16 transmembrane helixes in its domains A and C. The single copy CfCHS1 gene expressed during each of the larval molts from the 3rd to the 6th instar. The gene expressed highly and periodically in the epidermis during each of molts, whereas no transcripts were detected in the midgut and fat body. 20-hydroxyecdysone and the ecdysone agonist RH5992 suppressed CfCHS1 expression, whereas the juvenile hormone analog methoprene induced CfCHS1 expression. These results implicate that CfCHS1 is involved in the chitin synthase and new chitin formation during molting in the insect.
authors.affiliation: Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, India.
authors.forename: Dinakar R
authors.initials: DR
authors.lastname: Ampasala
authors.forename: Sichun
authors.initials: S
authors.lastname: Zheng
authors.forename: Dayu
authors.initials: D
authors.lastname: Zhang
authors.forename: Tim
authors.initials: T
authors.lastname: Ladd
authors.forename: Daniel
authors.initials: D
authors.lastname: Doucet
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Krell
authors.forename: Arthur
authors.initials: A
authors.lastname: Retnakaran
authors.forename: Qili
authors.initials: Q
authors.lastname: Feng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002686:Chitin / Q000502:physiology; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004441:Ecdysterone / Q000378:metabolism; D004817:Epidermis / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental; D006834:Hydrazines / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics; D008726:Methoprene / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D006834:Hydrazines; D019476:Insect Proteins; D002686:Chitin; D004441:Ecdysterone; D008726:Methoprene; D002687:Chitin Synthase; C082026:tebufenozide