title: Identification of proteins associated with ligand-activated estrogen receptor α in human breast cancer cell nuclei by tandem affinity purification and nano LC-MS/MS.
abstract: Estrogen receptor α (ER-α) is a key mediator of estrogen actions in breast cancer (BC) cells. Understanding the effects of ligand-activated ER-α in target cells requires identification of the molecular partners acting in concert with this nuclear receptor to transduce the hormonal signal. We applied tandem affinity purification (TAP), glycerol gradient centrifugation and MS analysis to isolate and identify proteins interacting with ligand-activated ER-α in MCF-7 cell nuclei. This led to the identification of 264 ER-associated proteins, whose functions highlight the hinge role of ER-α in the coordination of multiple hormone-regulated nuclear processes in BC cells.
authors.affiliation: Department of General Pathology, Second University of Naples, Napoli, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Tarallo
authors.forename: Angela
authors.initials: A
authors.lastname: Bamundo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Nassa
authors.forename: Ernesto
authors.initials: E
authors.lastname: Nola
authors.forename: Ornella
authors.initials: O
authors.lastname: Paris
authors.forename: Concetta
authors.initials: C
authors.lastname: Ambrosino
authors.forename: Angelo
authors.initials: A
authors.lastname: Facchiano
authors.forename: Marc
authors.initials: M
authors.lastname: Baumann
authors.forename: Tuula A
authors.initials: TA
authors.lastname: Nyman
authors.forename: Alessandro
authors.initials: A
authors.lastname: Weisz
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid / Q000379:methods*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D006801:Humans; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047628:Estrogen Receptor alpha