title: Improved functional activity of bone marrow derived circulating progenitor cells after intra coronary freshly isolated bone marrow cells transplantation in patients with ischemic heart disease.
abstract: OBJECTIVE
  There is growing evidence that intracoronary autologous bone marrow cells transplantation (BMCs-Tx) in patients with chronic myocardial infarction beneficially affects postinfarction remodelling. In this randomized controlled study we analyzed the influence of intracoronary autologous freshly isolated bone marrow cells transplantation by use of point of care system on cardiac function and on the functional activity of bone marrow derived circulating progenitor cells (BM-CPCs) in patients with ischemic heart disease (IHD).
  
  
  METHODS
  56 patients with IHD were randomized to either received freshly isolated BMC-Tx or a control group that did not receive cell therapy. The functional activity of BM-CPCs in peripheral blood (PB) was measured by migration assay and colony forming unit assay pre- and 3, 6 as well as 12 months after procedure. Global ejection fraction (EF) and infarct size area were determined by left ventriculography.
  
  
  RESULTS
  Intracoronary transplantation of autologous freshly isolated BMCs led to a significant reduction of infarct size and an increase of global EF as well as infarct wall movement velocity after 3 and 12 months follow-up compared to control group. The colony-forming capacity of BM-CPCs significantly increased 3, 6 and 12 months after cell therapy compared to pre BMCs-Tx and control group (CFU-E: p < 0.001, CFU-GM: p < 0.001). Likewise, we found significant increase of migratory response to stromal cell-derived factor 1 (SDF-1) and vascular endothelial growth factor (VEGF) after cell therapy compared to pre BMCs-Tx (SDF-1: p < 0.001, VEGF: p < 0.001) and to control (SDF-1: p < 0.001, VEGF: p < 0.001). There was no significant difference of migratory- and colony forming capacity between pre- and 3, 6, 12 months after coronary angiography in control group without cell therapy.
  
  
  CONCLUSIONS
  Intracoronary transplantation of autologous freshly isolated BMCs by use of point of care system may lead to improvement of BM-CPCs functional activity in peripheral blood, which might increase the regenerative potency in patients with IHD.
authors.affiliation: Department of Internal Medicine, Division of Cardiology, Rostock-University, Ernst Hydemann Str 6, 18055, Rostock, Germany. dr_g_turan@hotmail.com
authors.forename: R Goekmen
authors.initials: RG
authors.lastname: Turan
authors.forename: I
authors.initials: I
authors.lastname: Bozdag-T
authors.forename: J
authors.initials: J
authors.lastname: Ortak
authors.forename: S
authors.initials: S
authors.lastname: Kische
authors.forename: I
authors.initials: I
authors.lastname: Akin
authors.forename: H
authors.initials: H
authors.lastname: Schneider
authors.forename: C H
authors.initials: CH
authors.lastname: Turan
authors.forename: T C
authors.initials: TC
authors.lastname: Rehders
authors.forename: M
authors.initials: M
authors.lastname: Rauchhaus
authors.forename: T
authors.initials: T
authors.lastname: Kleinfeldt
authors.forename: C
authors.initials: C
authors.lastname: Belu
authors.forename: M
authors.initials: M
authors.lastname: Brehm
authors.forename: S
authors.initials: S
authors.lastname: Yokus
authors.forename: S
authors.initials: S
authors.lastname: Steiner
authors.forename: K
authors.initials: K
authors.lastname: Sahin
authors.forename: C A
authors.initials: CA
authors.lastname: Nienaber
authors.forename: H
authors.initials: H
authors.lastname: Ince
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D016026:Bone Marrow Transplantation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D019095:Point-of-Care Systems; D011446:Prospective Studies; D012038:Regeneration / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D016276:Ventricular Function; D055815:Young Adult