title: Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening.
abstract: A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-β1 and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.
authors.affiliation: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Mingke
authors.initials: M
authors.lastname: Wang
authors.forename: Huiqin
authors.initials: H
authors.lastname: Sun
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yongping
authors.initials: Y
authors.lastname: Su
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Zou
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D003001:Cloning, Molecular; D015723:Gene Library; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007763:Lac Operon / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D007093:Imidazoles; C557511:Mgt-6 protein, mouse; D009687:Nuclear Proteins; D011725:Pyridines; D053773:Transforming Growth Factor beta1; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D020929:Mitogen-Activated Protein Kinase Kinases; C093642:SB 203580