title: Amisulpride-induced seizurogenic effect: a potential role of opioid receptor-linked transduction systems.
abstract: This study was designed to investigate the role of opioid receptors, gamma-aminobutyric acid (GABA) receptors, mast cells and histamine receptors (H(1) subtype) in the seizurogenic effect of amisulpride on mice. A single injection of amisulpride (180 mg/kg) was employed to evaluate the seizurogenicity of the drug in mice. Seizures were assessed in terms of a composite seizure severity score (SSS), time of the onset of straub-like tail, onset of jerky movements of whole body, convulsions and death. Amisulpride administration (180 mg/kg) induced a significant pro-convulsant effect in mice as measured in terms of the SSS (21.12 ± 2.71) and a significant decrease in the time latency of the onset of straub-like tail (132.45 ± 12.31), jerky movements of whole body (153.28 ± 14.12), convulsions (184.97 ± 13.11) and death (100%). Moreover, prior administration of naloxone, cetrizine, sodium cromoglycate and gabapentin, respectively, attenuated this seizurogenic activity that amisulpride exerted on mice (p < 0.05). Therefore, it may be suggested that amisulpride exerts a seizurogenic effect on mice possibly via an opioid receptor activation-dependent release of histamine from the mast cells and a simultaneous inhibition of GABA release.
authors.affiliation: Chitkara College of Pharmacy, Chandigarh-Patiala National Highway, Rajpura, Punjab, India.
authors.forename: Ashish K
authors.initials: AK
authors.lastname: Rehni
authors.forename: Thakur Gurjeet
authors.initials: TG
authors.lastname: Singh
authors.forename: Prem
authors.initials: P
authors.lastname: Chand
mesh_terms: D000588:Amines / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000077582:Amisulpride; D000704:Analysis of Variance; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D003509:Cyclohexanecarboxylic Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000077206:Gabapentin; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009270:Naloxone / Q000494:pharmacology; D018079:Receptors, GABA / Q000378:metabolism*; D011969:Receptors, Histamine H1 / Q000378:metabolism; D011957:Receptors, Opioid / Q000187:drug effects*; D012640:Seizures / Q000139:chemically induced*; D013469:Sulpiride / Q000031:analogs & derivatives* / Q000633:toxicity; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D000588:Amines; D018927:Anti-Asthmatic Agents; D003509:Cyclohexanecarboxylic Acids; D018079:Receptors, GABA; D011969:Receptors, Histamine H1; D011957:Receptors, Opioid; D009270:Naloxone; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D013469:Sulpiride; D000077582:Amisulpride; D004205:Cromolyn Sodium