title: Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites.
abstract: Synthetic biology aims to design and build new biological systems with desirable properties, providing the foundation for the biosynthesis of secondary metabolites. The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology. However, there are advantages of using a deleted host, and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genome-reduced microbes. This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ashforth
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002513:Cephamycins / Q000096:biosynthesis; D004852:Epoxy Compounds / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D053263:Gene Regulatory Networks; D005818:Genetic Engineering / Q000379:methods*; D005827:Genetics, Microbial; D016678:Genome; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism; D013307:Streptomycin / Q000096:biosynthesis; D058615:Synthetic Biology
chemical_list: D002513:Cephamycins; D004852:Epoxy Compounds; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C515348:amorpha-4,11-diene; D013307:Streptomycin