title: Human rotavirus genotypes detection among hospitalized children, a study in Tehran, Iran.
abstract: BACKGROUND
  Human rotavirus genotypes G1-G4, G9, P[4] and P[8] are major worldwide causes of acute gastroenteritis in children. Rotavirus genotype G1P[8] is predominant in many countries. In this study, the genotypic diversity of group A rotaviruses were detected in children <5 years of age who were treated for dehydration and diarrhea in Tehran, Iran from October 2004 to September 2008.
  
  
  METHODS
  A total of 700 stool specimens were collected from children and assessed for the presence of rotaviruses by the dsRNA-PAGE technique. G and P typing of the positive samples were performed by semi-nested multiplex RT-PCR.
  
  
  RESULTS
  Rotaviruses were isolated in 19% of samples. A total of 14 rotavirus dsRNA different electrophoretypes were detected. The predominant genotype was G1 (76.3%), followed by G4 (11.5%), G8 (0.8%), P[4] (9.2%) and P[8] (66.4%), respectively. In mixed type samples, the majority were of genotype G1P[8] (53.4%), followed by G1P[4] (9.2%) and G4P[8] (4.6%). Mixed types consisted of 3.1% of the total sample followed by G1G2/-P (1.5%), G1G4P[4] (0.8%) and G1G4P[8] (0.8%).
  
  
  CONCLUSIONS
  In this study, a high prevalence of the G1P[8] genotype was determined to be the cause of childhood gastroenteritis in Tehran, Iran. The sequence of G and P genotypes showed high levels of similarity to strains from other Asian countries. Our data will be useful for future vaccine formulation in Iran.
authors.affiliation: Department of Virology, Pasteur Institute, Tehran, Iran. smodaress@pasteur.ir
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Modaress
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Rahbarimanesh
authors.forename: Rosita
authors.initials: R
authors.lastname: Edalat
authors.forename: Amir
authors.initials: A
authors.lastname: Sohrabi
authors.forename: Shahab
authors.initials: S
authors.lastname: Modarres
authors.forename: Hosna
authors.initials: H
authors.lastname: Gomari
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Motamedirad
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Sayari
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D005759:Gastroenteritis / Q000821:virology; D005838:Genotype; D006776:Hospitals, Pediatric; D006801:Humans; D015994:Incidence; D007223:Infant; D007492:Iran; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D017423:Sequence Analysis, RNA
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus