title: Serum levels of IL-1β, IL-6 and TNF-α in rats fed with Kashin-Beck disease-affected diet.
abstract: OBJECTIVE
  To investigate the serum level of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α in rats which have been fed with Kashin-Beck disease (KBD) epidemic district food.
  
  
  METHODS
  Two hundred and twenty Wistar rats were divided into five groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T-2 toxin; group C was fed with a low-nutrition diet and T-2 toxin; group D was fed with a low-nutrition diet; and group E was fed with a KBD-affected diet. The serum bioactivity of IL-1β, IL-6 and TNF-α were tested by enzyme-linked immeunosorbent assay.
  
  
  RESULTS
  After 4 weeks, the epiphyseal plate showed more obvious necrosis of chondrocytes in groups B, C and E. Among the KBD-affected feed, normal feed combined with T-2 toxin and low protein combined with T-2 toxin, KBD-affected feed rats had the highest serum levels, and normal feed combined with T-2 toxin group was the lowest. Although the IL-1β, IL-6 and TNF-α levels were no different in the KBD-affected feed compared to low protein combined with T-2 toxin, there were significant differences compared to normal feed combined with the T-2 toxin (P < 0.05).
  
  
  CONCLUSIONS
  Serum levels of IL-1β, IL-6 and TNF-α metabolism are altered in the KBD model rats. This effect is relatively similar to the low-nutrition diet combined with the T-2 toxin, which means low-nutrition diet may be involved in the aetiology of KBD.
authors.affiliation: Orthopedic Department West China Hospital of Sichuan University, Chengdu, Sichuan, China.
authors.forename: Denglu
authors.initials: D
authors.lastname: Yan
authors.forename: Pengde
authors.initials: P
authors.lastname: Kang
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Zongke
authors.initials: Z
authors.lastname: Zhou
authors.forename: Lijun
authors.initials: L
authors.lastname: Duan
authors.forename: Fuxing
authors.initials: F
authors.lastname: Pei
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D019902:Chondrocytes / Q000473:pathology; D018753:Diet, Protein-Restricted; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005516:Food Microbiology; D006132:Growth Plate / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D057767:Kashin-Beck Disease / Q000097:blood / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D008297:Male; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D013605:T-2 Toxin; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D015415:Biomarkers; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D013605:T-2 Toxin