title: Regulation of resident and newcomer insulin granules by calcium and SNARE proteins.
abstract: Insulin, stored in large dense core granules, is biphasically exocytosed by glucose stimulation in pancreatic beta-cells. Several molecules, such as SNARE proteins, and Ca2+ ion are involved in the regulation of insulin exocytosis. Indeed, studies using gene targeting mice revealed critical roles of SNARE proteins and their accessory proteins, which may be associated with diabetes mellitus. In particular, the total internal reflection fluorescent (TIRF) imaging technique shed new light on the molecular mechanism of the insulin exocytotic process. In this review we discuss the mechanism of insulin exocytosis mainly from a point of view of imaging techniques.
authors.affiliation: Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Kyota
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Mica
authors.initials: M
authors.lastname: Ohara-Imaizumi
authors.forename: Shinya
authors.initials: S
authors.lastname: Nagamatsu
mesh_terms: D002118:Calcium / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005089:Exocytosis / Q000502:physiology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008856:Microscopy, Fluorescence; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D050600:SNARE Proteins / Q000502:physiology*; D022142:Secretory Vesicles / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism
chemical_list: D007328:Insulin; D050600:SNARE Proteins; D058539:Phosphatidylinositol 3-Kinase; D020830:rac1 GTP-Binding Protein; D002118:Calcium