title: Quantum dot based immunosensor using 3D circular microchannels fabricated in PDMS.
abstract: Microchannel is basic functional component of microfluidic chip and every step-forward of its construction technique has been receiving concern all over the world. The present work describes a novel, rapid and simple fabrication technique for building 3D microchannels in poly(dimethyl siloxane) (PDMS) elastomer. These microchannels were used for rapid detection of antigens (E. coli) by quantum dot (QD) based approach. Luminescent QD (CdTe) were synthesized by aqueous method and characterized using high resolution transmission electron microscopy (HRTEM), fluorescence spectroscopy and X-ray diffraction (XRD). The QDs were functionalized with anti-E. coli antibodies for immuno-detection. The reported process allowed easier and faster method of fabrication of circular 3D micochannels and demonstrated their potential use in an immuno-biosensor device.
authors.affiliation: Center for Sensor Studies, University of Pune, Ganeshkhind road, Pune 411 007, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Morarka
authors.forename: Shailaja
authors.initials: S
authors.lastname: Agrawal
authors.forename: Sonia
authors.initials: S
authors.lastname: Kale
authors.forename: Anup
authors.initials: A
authors.lastname: Kale
authors.forename: Satish
authors.initials: S
authors.lastname: Ogale
authors.forename: Kishore
authors.initials: K
authors.lastname: Paknikar
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Bodas
mesh_terms: D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized; D000942:Antigens, Bacterial / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds; D004129:Dimethylpolysiloxanes; D004926:Escherichia coli / Q000276:immunology; D046210:Microfluidic Analytical Techniques; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D055616:Microtechnology; D036103:Nanotechnology; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013691:Tellurium; D014961:X-Ray Diffraction
chemical_list: D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized; D000942:Antigens, Bacterial; D019187:Cadmium Compounds; D004129:Dimethylpolysiloxanes; C013830:baysilon; D013691:Tellurium; C028337:cadmium telluride