title: [Mechanism of multiple organ injury subsequent to acute renal failure with respect to membrane pump activity in rabbits].
abstract: OBJECTIVE
  To observe the changes in membrane pump activity of kidney, myocardium and pancreas in rabbits with acute renal failure (ARF) in rabbits, and inquire into the mechanism of multiple organ injury subsequent to ARF.
  
  
  METHODS
  Forty-two rabbits were randomly divided into control group, HgCl(2) group and glycerine group, and the latter two groups were subdivided into 12, 24, 48-hour subgroups, with 6 rabbits in each group. The ARF model was reproduced by hypodermic injection 1% HgCl(2) (1.3 ml/kg) in HgCl(2) group, or intramuscularly injection 50% glycerine (10 ml/kg) in glycerine group, respectively. At different time points, the kidney, myocardium and pancreas were harvested, and homogenates of them were prepared. The ATPase activities of different organ homogenates were determined.
  
  
  RESULTS
  It showed that the activities of Na(+)-K(+)-ATPase, Ca(2+)-ATPase, Ca(2+)-Mg(2+)-ATPase of renal homogenate in two model groups were reduced gradually with worsening of renal function, and they became lowest at 48 hours [(0.84±0.16), (0.52±0.17), (0.45±0.09) μmol×mg(-1)×h(-1) in HgCl(2) group; (0.85±0.22), (0.49±0.21), (0.54±0.17) μmol×mg(-1) ×h(-1)in glycerine group]. The respective activities of Na (+)-K (+)-ATPase, Ca (2+)-ATPase, Mg(2+)-ATPase, Ca(2+)-Mg(2+)-ATPase of myocardium and pancreas homogenates in two model groups were reduced gradually following depression of renal function, and they became lowest at 48 hours [(0.56±0.11), (0.51±0.19), (0.55±0.19), (0.37±0.19) μmol×mg(-1)×h(-1) in HgCl(2) group and (0.52±0.19), (0.62±0.10), (0.61±0.16), (0.54±0.10) μmol×mg(-1) ×h(-1) in myocardium homogenate of glycerine group; (0.81±0.12), (0.71±0.15), (0.73±0.18), (0.62±0.16) μmol×mg(-1) ×h(-1) in HgCl(2) group and (0.72±0.13), (0.57±0.18), (0.66±0.14), (0.59±0.23) μmol×mg(-1) ×h(-1) in pancreas homogenate of glycerine group], there was statistical difference compared with control group (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  The mechanism of myocardial and pancreatic injury subsequent to ARF might be related to reduction of the activity of cell membrane pump.
authors.affiliation: Department of Pathophysiology, Hebei North University, Zhangjiakou 075029, Hebei, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Han
authors.forename: Bao-liang
authors.initials: BL
authors.lastname: Li
authors.forename: Chun-yu
authors.initials: CY
authors.lastname: Niu
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Hou
authors.forename: Zi-gang
authors.initials: ZG
authors.lastname: Zhao
mesh_terms: D058186:Acute Kidney Injury / Q000150:complications / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D009102:Multiple Organ Failure / Q000209:etiology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D011817:Rabbits
chemical_list: D000251:Adenosine Triphosphatases