title: The effect of human bone marrow stroma-derived heparan sulfate on the ex vivo expansion of human cord blood hematopoietic stem cells.
abstract: OBJECTIVE
  In order to address cell dose limitations associated with the use of cord blood hematopoietic stem cell (HSC) transplantation, we explored the effect of bone marrow stroma-derived heparan sulfate (HS) on the ex vivo expansion of HSCs.
  
  
  METHODS
  Heparan sulfate was isolated and purified from the conditioned media of human bone marrow stromal cells and used for the expansion of cord blood-derived CD34(+) cells in the presence of a cocktail of cytokines.
  
  
  RESULTS
  The number of myeloid lineage-committed progenitor cells was increased at low dosage of HS as illustrated by an increase in the total number of colony-forming cells (CFC) and colonies of erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) precursors. Notably, the stroma-derived HS did not alter the growth of CD34(+) HSCs or negatively affect the levels of various HSC phenotypic markers after expansion.
  
  
  CONCLUSIONS
  This study shows that HS secreted into solution by stromal cells has the capacity to support hematopoietic cytokines in the maintenance and expansion of HSCs. The incorporation of stroma-derived HS as a reagent may improve the efficacy of cord blood HSC transplantation by enhancing the number of committed cells and accelerating the rate of engraftment.
authors.affiliation: Stem Cells and Tissue Repair Group, Institute of Medical Biology, Agency for Science, Technology and Research, 8A Biomedical Grove, #06-06 Immunos, Singapore, Singapore.
authors.forename: Diah S
authors.initials: DS
authors.lastname: Bramono
authors.forename: David A
authors.initials: DA
authors.lastname: Rider
authors.forename: Sadasivam
authors.initials: S
authors.lastname: Murali
authors.forename: Victor
authors.initials: V
authors.lastname: Nurcombe
authors.forename: Simon M
authors.initials: SM
authors.lastname: Cool
mesh_terms: D001854:Bone Marrow Cells / Q000737:chemistry; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods; D017077:Culture Media, Conditioned; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D006497:Heparan Sulfate / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D055015:Megakaryocyte-Erythroid Progenitor Cells / Q000166:cytology / Q000187:drug effects; D013234:Stem Cells; D017154:Stromal Cells / Q000737:chemistry
chemical_list: D017077:Culture Media, Conditioned; D006025:Glycosaminoglycans; D006497:Heparan Sulfate