title: Pectin methylesterase is induced in Arabidopsis upon infection and is necessary for a successful colonization by necrotrophic pathogens.
abstract: The ability of bacterial or fungal necrotrophs to produce enzymes capable of degrading pectin is often related to a successful initiation of the infective process. Pectin is synthesized in a highly methylesterified form and is subsequently de-esterified in muro by pectin methylesterase. De-esterification makes pectin more susceptible to the degradation by pectic enzymes such as endopolygalacturonases (endoPG) and pectate lyases secreted by necrotrophic pathogens during the first stages of infection. We show that, upon infection, Pectobacterium carotovorum and Botrytis cinerea induce in Arabidopsis a rapid expression of AtPME3 that acts as a susceptibility factor and is required for the initial colonization of the host tissue.
authors.affiliation: Department of Land, Environment, Agriculture and Forestry, University of Padua, Legnaro (PD) Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Raiola
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lionetti
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Elmaghraby
authors.forename: Peter
authors.initials: P
authors.lastname: Immerzeel
authors.forename: Ewa J
authors.initials: EJ
authors.lastname: Mellerowicz
authors.forename: Giovanni
authors.initials: G
authors.lastname: Salvi
authors.forename: Felice
authors.initials: F
authors.lastname: Cervone
authors.forename: Daniela
authors.initials: D
authors.lastname: Bellincampi
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020171:Botrytis / Q000254:growth & development / Q000472:pathogenicity*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010368:Pectins / Q000378:metabolism; D016973:Pectobacterium carotovorum / Q000254:growth & development / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000382:microbiology
chemical_list: D029681:Arabidopsis Proteins; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase