title: [Study on the relation between anticancer drug-sensitivity and differentional expression of anticancer drug sensitivity related genes in non-small cell lung cancer cell lines].
abstract: BACKGROUND
  In order to enhance the chemotherapeutic efficacy of advanced lung cancer and to practise the individualized treatment, it is necessary to find out the difference of anticancer drug-sensitivity in lung cancer cells. Comparing the array profile of related gene of anticancer drug-sensitivity between non-small cell lung cancer (NSCLC) cell lines and immortal human bronchial epithelial cell line BET2A, the difference of expression of related genes of anticancer drug sensitivity was detected.
  
  
  METHODS
  With the technique of cDNA macroarray, the different related gene proceeding of anticancer drug sensitivity expression was analysed in 6 NSCLC cell lines and BET2A cell line. RT-PCR was used to reconfirm the results.
  
  
  RESULTS
  Seventy-three genes which were differentially expressed were found from 1291 candidate genes, and there were 45 genes upregulated, and 28 genes downregulated. The results of RT-PCR were consistent with those of cDNA macroarray.
  
  
  CONCLUSIONS
  The main reason of different sensitivity might be the difference of related gene of anticancer drug sensitivity expression. The results of this study provide new targets for reversing multiple drug-resistance, and they also provide experimental evidence to develop some new drugs and to realize the individualized treatment clinically.
authors.affiliation: Department of Internal Medicine, Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150040, P.R.China.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Guangjie
authors.initials: G
authors.lastname: Sui