title: An endophilin-dynamin complex promotes budding of clathrin-coated vesicles during synaptic vesicle recycling.
abstract: Clathrin-mediated vesicle recycling in synapses is maintained by a unique set of endocytic proteins and interactions. We show that endophilin localizes in the vesicle pool at rest and in spirals at the necks of clathrin-coated pits (CCPs) during activity in lamprey synapses. Endophilin and dynamin colocalize at the base of the clathrin coat. Protein spirals composed of these proteins on lipid tubes in vitro have a pitch similar to the one observed at necks of CCPs in living synapses, and lipid tubules are thinner than those formed by dynamin alone. Tubulation efficiency and the amount of dynamin recruited to lipid tubes are dramatically increased in the presence of endophilin. Blocking the interactions of the endophilin SH3 domain in situ reduces dynamin accumulation at the neck and prevents the formation of elongated necks observed in the presence of GTPγS. Therefore, endophilin recruits dynamin to a restricted part of the CCP neck, forming a complex, which promotes budding of new synaptic vesicles.
authors.affiliation: Department of Neuroscience, DBRM, Karolinska Institutet, 17177 Stockholm, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Sundborger
authors.forename: Cynthia
authors.initials: C
authors.lastname: Soderblom
authors.forename: Olga
authors.initials: O
authors.lastname: Vorontsova
authors.forename: Emma
authors.initials: E
authors.lastname: Evergren
authors.forename: Jenny E
authors.initials: JE
authors.lastname: Hinshaw
authors.forename: Oleg
authors.initials: O
authors.lastname: Shupliakov
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D022163:Clathrin-Coated Vesicles / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034284:Dynamin I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007798:Lampreys; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013569:Synapses / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013572:Synaptic Vesicles / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C107640:SH3GL2 protein, human; D034284:Dynamin I