title: [Determination of 25(R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside in Liriope muscari from different habitats and different harvest time by HPLC-ELSD].
abstract: OBJECTIVE
  To develop an HPLC-ELSD method for the determination of 25 (R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside in the tuberous roots of Liriope muscari from different habitats and different harvest time.
  
  
  METHODS
  A Shimadzu C18 column (4.6 mm x 150 mm, 5 microm) with a solvent system consisting of acetonirile-water (46: 54) was used, and detected by ELSD. The temperature of drift tube was 94 degrees C and the nebulizer nitrogen flow rate was 2.5 L x min(-1).
  
  
  RESULTS
  The calibration curve of 25 (R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside showed good linearity in the range of 1.02-12.228 microg and the average recovery was 100.80%, with RSD of 1.8%. 10 batches of L. muscari from different habitats were analyzed, and the contents were 0.25% - 0.41%. The contents of 15 batches from different harvest time were 0.13%-0.38%.
  
  
  CONCLUSIONS
  The method is simple, rapid and sensitive, and can be used for determination of 25 (R, S) ruscogenin 1-O-[beta-D-glucopyranosyl (1 --> 2)] [beta-D-xylopyranosyl (1 --> 3)] beta-D-fucopyranoside in L. muscari. It provides the valuable basis for quality assessment of L. muscari.
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China.
authors.forename: Zhengfang
authors.initials: Z
authors.lastname: Hu
authors.forename: Chang
authors.initials: C
authors.lastname: Jiang
authors.forename: Minjian
authors.initials: M
authors.lastname: Qin
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.forename: Boyang
authors.initials: B
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017753:Ecosystem; D019668:Liliaceae / Q000737:chemistry*; D031424:Liriope Plant / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D015394:Molecular Structure; D028321:Plant Preparations / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry* / Q000502:physiology; D018514:Plant Structures / Q000737:chemistry; D012503:Saponins / Q000737:chemistry; D013150:Spirostans / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D014315:Triterpenes / Q000302:isolation & purification*
chemical_list: D028321:Plant Preparations; D012503:Saponins; D013150:Spirostans; D014315:Triterpenes; C012541:ruscogenin