title: Flux-based analysis of sulfur metabolism in desulfurizing strains of Rhodococcus erythropolis.
abstract: Rhodococcus erythropolis has been studied widely for potential applications in biodesulfurization. Previous works have been largely experimental with an emphasis on the characterization and genetic engineering of desulfurizing strains for improved biocatalysis. A systems modeling approach that can complement these experimental efforts by providing useful insights into the complex interactions of desulfurization reactions with various other metabolic activities is absent in the literature. In this work, we report the first attempt at reconstructing a flux-based model to analyze sulfur utilization by R. erythropolis. The model includes the 4S pathway for dibenzothiophene (DBT) desulfurization. It predicts closely the growth rates reported by two independent experimental studies, and gives a clear and comprehensive picture of the pathways that assimilate the sulfur from DBT into biomass. In addition, it successfully elucidates that sulfate promotes higher cell growth than DBT and its presence in the medium reduces DBT desulfurization rates. A study using eight carbon sources suggests that ethanol and lactate yield higher cell growth and desulfurization rates than citrate, fructose, glucose, gluconate, glutamate, and glycerol.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Aggarwal
authors.forename: Iftekhar A
authors.initials: IA
authors.lastname: Karimi
authors.forename: Dong Yup
authors.initials: DY
authors.lastname: Lee
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism; D008954:Models, Biological; D015233:Models, Statistical; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism
chemical_list: D013876:Thiophenes; D013455:Sulfur; C016366:dibenzothiophene