title: [SARA expression in high glucose induced epithelium to mesenchymal transition of proximal tubule cells].
abstract: OBJECTIVE
  To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation (SARA).
  
  
  METHODS
  Protein expression of vimentin, Zona occludens-1(ZO-1), and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR.
  
  
  RESULTS
  After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner.
  
  
  CONCLUSIONS
  High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.
authors.affiliation: Department of Nephrology, Second Xiangya Hospital, Changsha 410011, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Tang
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ling
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
authors.forename: Youming
authors.initials: Y
authors.lastname: Peng
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Shaobin
authors.initials: S
authors.lastname: Duan
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology*; D010750:Phosphoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism; D062826:Zonula Occludens-1 Protein
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C569042:TJP1 protein, human; D053773:Transforming Growth Factor beta1; D014746:Vimentin; D062826:Zonula Occludens-1 Protein; C116420:ZFYVE16 protein, human; D012697:Serine Endopeptidases; D005947:Glucose