title: [Effect of Salvia miltiorrhiza monomer IH764-3 on MMP-13 and TIMP-1 by downregulating the expression of focal adhesion kinase in hepatic stellate cell stimulated by H2O2].
abstract: OBJECTIVE
  To investigate the effect of IH764-3 on the expression of MMP-13 and TIMP-1 by H2O2-stimulated hepatic stellate cell and the alteration of FAK during this process.
  
  
  METHODS
  The expression of MMP-13 and FAK mRNA was examined by RT-PCR. TIMP-1 mRNA was analyzed by in-situ hybridization. FAK and TIMP-1 were evaluated at protein level through Western blotting method.
  
  
  RESULTS
  Being incubated for 2 h, compared with control group, MMP-13 mRNA was upregulated by IH764-3, but TIMP-1 transcription was reduced in a dose-dependent manner, accompanied with the decrease of FAK mRNA. The expression of TIMP-1 and FAK protein in HSC also decreased after being exposed by IH764-3 for 24 h.
  
  
  CONCLUSIONS
  IH764-3 can induce the expression of MMP-13 and inhibit the expression of TIMP-1. Down-regulating the expression of FAK mRNA may be one of its mechanisms.
authors.affiliation: Department of Gastroenterology, the Second Hospital of Hebei Medical Univesity, Shijiazhuang 050000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Jiang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Zhang
authors.forename: Dong-qiang
authors.initials: DQ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000009:adverse effects; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; C079053:IH 764-3; D019715:Tissue Inhibitor of Metalloproteinase-1; D006861:Hydrogen Peroxide; D051416:Focal Adhesion Protein-Tyrosine Kinases; D053509:Matrix Metalloproteinase 13; C507083:Mmp13 protein, rat