title: [Screening differentially expressed genes in the process of vascular smooth muscle cell calcification in vitro].
abstract: OBJECTIVE
  The process of vascular calcification involves various genetic alterations which may play a very important role in the vascular calcification. Vascular smooth muscle cells undoubtedly composed the main part of vascular cells, and are involved in vascular calcification. So bovine artery smooth muscle cell (BASMC) was used to investigate the gene changes during BASMC's calcification.
  
  
  METHODS
  Bovine artery smooth muscle cells cultured in vitro was induced calcified by beta-Glycerophosphate (beta-GP). Using DD-PCR technique to screening differentially expressed genes and those differentially expressed bands were reexamined by reverse Northern blot. All the ESTs were sequenced and BLAST with GenBank.
  
  
  RESULTS
  Total 65 cDNAs were isolated as differentially expressed genes and 40 of them were successfully reamplified. Using reverse-Northern blot, seven of these 40 cDNAs were reproducibly expressed differentially between the two cells. Three of them are new bands and have not been reported before.
  
  
  CONCLUSIONS
  This is the first time using DD-PCR to screen differentially expressed genes of BASMC calcification. Seven related ESTs were identified relating to BASMC calcification.
authors.affiliation: Department of senior citizens, The 304 Hospital of PLA, Beijing 100037, China.
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Wang
authors.forename: Shi-wen
authors.initials: SW
authors.lastname: Wang
authors.forename: Lan-ying
authors.initials: LY
authors.lastname: Chen
authors.forename: Ping-sheng
authors.initials: PS
authors.lastname: Li
authors.forename: Xiu-yun
authors.initials: XY
authors.lastname: Ding
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D020224:Expressed Sequence Tags; D014644:Genetic Variation; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D061205:Vascular Calcification / Q000235:genetics / Q000378:metabolism* / Q000473:pathology