title: [Effect on hyperplasia of VSMCS in rat by the plasmid containing the short hairpin RNA of AT1R].
abstract: OBJECTIVE
  RNA interference is a new technology that inhibit effectively of the expression the specific genes. The present study was designed to investigate whether the plasmid containing the short hairpin RNA (shRNA) of angiotensin II type 1 receptor (AT1R) can inhibit the hyperplasia of VSMCs in rat.
  
  
  METHODS
  The plasmids containing the shRNA of AT1R were constructed, and transfected vascular smooth muscle cell (VSMC) to detect the effect on the AT1R expression by RT-PCR and Western blot, and detect the hyperplasia of VSMCs by trypan blues training and MTT.
  
  
  RESULTS
  The plasmids was certified to be in the right rank, and there was significant difference (P < 0.01) in the expression of AT1R mRNA and protein between the plasmid transfected group and the control group. There was significant difference (P < 0.01) in the hyperplasia of VSMCs between the plasmid transfected adding Ang II group and the control group.
  
  
  CONCLUSIONS
  The plasmids containing the shRNA of AT1R have the effect of RNAi, and inhibit the hyperplasia of VSMCs induced by Ang II in rat.
authors.affiliation: Department of Vascular Surgery, The First Hospital Affiliated to China Medical University, Shenyang 110001, China.
authors.forename: Cheng-lin
authors.initials: CL
authors.lastname: Sun
authors.forename: Zhi-quan
authors.initials: ZQ
authors.lastname: Duan
authors.forename: Shi-jie
authors.initials: SJ
authors.lastname: Xin
authors.forename: Zhong-cheng
authors.initials: ZC
authors.lastname: Feng
authors.forename: Jing-hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Ling-yu
authors.initials: LY
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D044140:Receptor, Angiotensin, Type 1