title: Performance evaluation and phylogenetic characterization of anaerobic fluidized bed reactors using ground tire and pet as support materials for biohydrogen production.
abstract: This study evaluated two different support materials (ground tire and polyethylene terephthalate [PET]) for biohydrogen production in an anaerobic fluidized bed reactor (AFBR) treating synthetic wastewater containing glucose (4000 mg L(-1)). The AFBR, which contained either ground tire (R1) or PET (R2) as support materials, were inoculated with thermally pretreated anaerobic sludge and operated at a temperature of 30°C. The AFBR were operated with a range of hydraulic retention times (HRT) between 1 and 8h. The reactor R1 operating with a HRT of 2h showed better performance than reactor R2, reaching a maximum hydrogen yield of 2.25 mol H(2)mol(-1) glucose with 1.3mg of biomass (as the total volatile solids) attached to each gram of ground tire. Subsequent 16S rRNA gene sequencing and phylogenetic analysis of particle samples revealed that reactor R1 favored the presence of hydrogen-producing bacteria such as Clostridium, Bacillus, and Enterobacter.
authors.affiliation: Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis km 235, CEP 13565-905 São Carlos, SP, Brazil.
authors.forename: Aruana Rocha
authors.initials: AR
authors.lastname: Barros
authors.forename: Maria Angela Tallarico
authors.initials: MA
authors.lastname: Adorno
authors.forename: Isabel Kimiko
authors.initials: IK
authors.lastname: Sakamoto
authors.forename: Sandra Imaculada
authors.initials: SI
authors.lastname: Maintinguer
authors.forename: Maria Bernadete Amâncio
authors.initials: MB
authors.lastname: Varesche
authors.forename: Edson Luiz
authors.initials: EL
authors.lastname: Silva
mesh_terms: D000693:Anaerobiosis; D001407:Bacillus / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003013:Clostridium / Q000378:metabolism; D004754:Enterobacter / Q000378:metabolism; D004867:Equipment Design; D005947:Glucose / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D010802:Phylogeny; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods*
chemical_list: D011093:Polyethylene Terephthalates; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D006859:Hydrogen; D005947:Glucose