title: Subcellular localization of the alphaherpesvirus serine/threonine kinase Us3 as a determinant of Us3 function.
abstract: The Us3 serine threonine kinases perform multiple roles in alphaherpesvirus infection and can localize to distinct subcellular compartments. Transient expression of Us3 in cells results in two dramatic alterations of the actin cytoskeleton: production of actin-based filamentous processes (FPs); and breakdown of actin stress fibres giving rise to rounded cell morphology. In our recent study on FPs induced by HSV-2 Us3, we noted that FP formation was diminished when HSV-2 Us3 was trapped within the nucleus following treatment of transfected cells with leptomycin B (LMB). This observation suggested that subcellular localization of Us3 could be a determinant of Us3-induced FP formation. Here, we review what is known regarding the effect of subcellular localization of Us3 on FP production and on actin stress fibre breakdown and discuss the potential significance of studies aimed at defining the requirements for subcellular localization of Us3.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, ON, CA.
authors.forename: Renée L
authors.initials: RL
authors.lastname: Finnen
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Banfield
mesh_terms: D000199:Actins / Q000378:metabolism*; D018138:Alphaherpesvirinae / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D000818:Animals; D002450:Cell Communication; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D002522:Chlorocebus aethiops; D003599:Cytoskeleton / Q000378:metabolism*; D018258:Herpesvirus 2, Human / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology* / Q000378:metabolism; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D014764:Viral Proteins; D017346:Protein Serine-Threonine Kinases; C084395:US3 protein, Human herpesvirus 2