title: [Measurement of nitrate and nitrite concentrations in biological fluids using high performance liquid chromatography].
abstract: OBJECTIVE
  New method to analyse nitrate and nitrite concentrations in saliva, serum and urine was developed using high performance liquid chromatography.
  
  
  RESULTS
  The whole isolation process was completed in less than 7 minutes, the determination linearity of nitrate and nitrite were 0.7 ng-100 ng and 5 ng-100 ng, respectively. MINIMUM: Detectable limits of nitrate and nitrite were 0.3 ng and 2 ng, respectively. Nitrate recovery ratio was 99%-102% and nitrite recovery ratio was 99%-104%. The RSD of nitrate and nitrite was 0.8% and 1.7%, respectively.
  
  
  CONCLUSIONS
  In comparison with other methods available, the present method seems to be simpler, more sensitive and specific.
authors.affiliation: Department of Neurobiology, Capital University of Medical Sciences, Beijing 100054, China.
authors.forename: Xiu-yu
authors.initials: XY
authors.lastname: Cui
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Lu
mesh_terms: D001826:Body Fluids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*
chemical_list: D009566:Nitrates; D009573:Nitrites