title: [Analysis of outer membrane proteins in various strains of Porphyromonas gingivalis by surface enhanced laser desorption/ionization time-of-flight mass spectrometry].
abstract: OBJECTIVE
  To analyze the common outer membrane proteins (OMP) from Porphyromonas gingivalis (Pg) by surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and to provide antigens for the subsequent experiments in screening vaccine for periodontitis therapy.
  
  
  METHODS
  Four strains of Pg were cultured under anaerobic conditions. The common OMP was extracted through ultracentrifugation and SELDI-TOF-MS was employed to detect the expressions of proteomes by chip H(50). The data was analyzed by Biomarker Wizard.
  
  
  RESULTS
  Four kinds of strains of OMP fingerprint spectrum were obtained. Seventy-one proteins of PgATCC33277, 74 proteins of PgW83, 76 proteins of PgW301 and 72 proteins of Pg381 were captured by chip H(50). Thirteen common proteins were identified according to fingerprint spectrum. There was only 1 of the 13 common proteins identified in NCBI protein bank.
  
  
  CONCLUSIONS
  SELDI-TOF-MS has good reproducibility and high sensibility and can be used to identify the common OMP of Pg. The 13 proteins have a potential value in the screening vaccine candidate antigen sites for periodontitis.
authors.affiliation: Research Center for Stomatology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Sun
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Sun
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Guo-zhou
authors.initials: GZ
authors.lastname: Rao
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Gou
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis*; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000276:immunology*; D011506:Proteins; D020543:Proteome; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000942:Antigens, Bacterial; D008565:Membrane Proteins; D011506:Proteins; D020543:Proteome