title: [Isolation, incubation and differentiation of pancreatic islet-derived progenitor cells from newborn SD rats].
abstract: OBJECTIVE
  To isolate, culture pancreatic progenitor cells derived from islets of newborn rats, and to observe the effect of GLP-1 (7-36) NH2 on islet progenitor differentiation.
  
  
  METHODS
  Islets were isolated purified, islet progenitor cells were isolated and proliferated in the modified RPMI-1640 medium supplemented with 20 microg/L bFGF and 20 microg/L EGF, then were differentiated with 20 nmol/L GLP-1 (7-36) NH2. The properties of islet progenitor cells were identified primarily by hybridization in situ, immunocytochemistry, dithizone (DTZ)-staining, and radioimmunoassay before and after differentiation.
  
  
  RESULTS
  Islet progenitor cells did not express PDX-1, insulin and somatostatin, but nestin. After differentiation, a portion of cells expressing PDX-1, insulin mRNA, insulin, somatostatin, and nestin, islet-like cell clusters (ICCs) were formed, DTZ-stained cells were in peripheral region of it. Insulin release was markedly greater in media harvested after differentiation of 3 weeks.
  
  
  CONCLUSIONS
  A kind of progenitor cells exists in pancreatic islet of newborn SD rats, could be expanded continuously. GLP-1 (7-36) NH2 could differentiate pancreatic islet-derived progenitor cells to form ICCs capable of insulin secretion.
authors.affiliation: Department of Neurology, Brain Hospital of Hunan Province, Changsha 410007, China.
authors.forename: Qing-Yong
authors.initials: QY
authors.lastname: Wang
authors.forename: Su-Fang
authors.initials: SF
authors.lastname: Liu
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Yun-Wei
authors.initials: YW
authors.lastname: Yao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D051381:Rats; D013234:Stem Cells / Q000166:cytology*
chemical_list: D052216:Glucagon-Like Peptide 1