title: Facile synthesis of Fe(3)O(4)@Al(2)O(3) core-shell nanoparticles and their application to the highly specific capture of heme proteins for direct electrochemistry.
abstract: In this study, magnetic core-shell Fe(3)O(4)@Al(2)O(3) nanoparticles (NPs) attached to the surface of a magnetic glassy carbon electrode (MGCE) were used as a functional interface to immobilize several heme proteins including hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP) for fabricating protein/Fe(3)O(4)@Al(2)O(3) film. Transmission electron microscope, UV-vis spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry were used to characterize the films. With the advantages of the magnetism and the excellent biocompatibility of the Fe(3)O(4)@Al(2)O(3) NPs, the protein/Fe(3)O(4)@Al(2)O(3) film could be easily fabricated in the present of external magnetic field, and well retained the bioactivity of the immobilized proteins, hence dramatically facilitated direct electron transfer of heme proteins and excellent electrocatalytic behaviors towards H(2)O(2) were demonstrated. The presented system avoids the complex synthesis for protecting Fe(3)O(4) NPs, supplies a facile, low cost and universal way to immobilize proteins, and is promising for construction of third-generation biosensors and other bio-magnetic induction devices.
authors.affiliation: Department of Chemistry and Institute for Advanced Study, Nanchang University, Xue Fu Da Dao 999, Nanchang 330031, China.
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Peng
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Liang
authors.forename: Jian-Ding
authors.initials: JD
authors.lastname: Qiu
mesh_terms: D000537:Aluminum Oxide; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D005290:Ferric Compounds; D006420:Hemeproteins / Q000302:isolation & purification; D006454:Hemoglobins; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000737:chemistry; D055767:Immobilized Proteins; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009211:Myoglobin; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D055672:Static Electricity
chemical_list: D005290:Ferric Compounds; D006420:Hemeproteins; D006454:Hemoglobins; D055767:Immobilized Proteins; D058185:Magnetite Nanoparticles; D009211:Myoglobin; C000499:ferric oxide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D000537:Aluminum Oxide