title: Fas and FasL expression in the spinal cord following cord hemisection in the monkey.
abstract: The changes of endogenous Fas/FasL in injured spinal cord, mostly in primates, are not well known. In this study, we investigated the temporal changes in the expression of Fas and FasL and explored their possible roles in the ventral horn of the spinal cord and associated precentral gyrus following T(11) spinal cord hemisection in the adult rhesus monkey. A significant functional improvement was seen with the time going on in monkeys subjected to cord hemisection. Apoptotic cells were also seen in the ventral horn of injured spinal cord with TUNEL staining, and a marked increase presents at 7 days post operation (dpo). Simultaneously, the number of Fas and FasL immunoreactive neurons in the spinal cords caudal and rostral to injury site and their intracellular optical density (OD) in the ipsilateral side of injury site at 7 dpo increased significantly more than that of control group and contralateral sides. This was followed by a decrease and returned to normal level at 60 dpo. No positive neurons were observed in precentral gyrus. The present results may provide some insights to understand the role of Fas/FasL in the spinal cord but not motor cortex with neuronal apoptosis and neuroplasticity in monkeys subjected to hemisection spinal cord injury.
authors.affiliation: Institute of Neuronlogic Desease, West China Hospital, Sichuan University, 610041, Chengdu, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Jia
authors.forename: Zou
authors.initials: Z
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Hui
authors.forename: Guan
authors.initials: G
authors.lastname: Yu-Guang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xin-Fu
authors.forename: You
authors.initials: Y
authors.lastname: Chao
authors.forename: Xiyang
authors.initials: X
authors.lastname: Yanbin
authors.forename: Zhan
authors.initials: Z
authors.lastname: Xi
authors.forename: Wang
authors.initials: W
authors.lastname: Jun
authors.forename: Heng
authors.initials: H
authors.lastname: Xin-Hua
authors.forename: Hen
authors.initials: H
authors.lastname: Xin-Hua
authors.forename: Wang
authors.initials: W
authors.lastname: Ting-Hua
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053222:Fas Ligand Protein / Q000378:metabolism*; D006614:Hindlimb / Q000294:innervation / Q000502:physiology; D020287:In Situ Nick-End Labeling; D008253:Macaca mulatta; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009044:Motor Cortex / Q000378:metabolism / Q000473:pathology; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor