title: [Lentiviral vector-mediated RNA interfere gene Nogo receptor to repair spinal cord injury].
abstract: OBJECTIVE
  To evaluate the effects of lentiviral vector-mediated RNA interfere gene Nogo receptor (NgR) of rat cortical neurons in repairing spinal cord injury.
  
  
  METHODS
  The recombinant-lentiviral vector with small inferring RNA siNgR199 which had been constructed was transfected into rat cortical neuron cells in vitro in 3 multiplicity of infection (MOI). The infection rate was determined with fluorescent microscope, and the target gene was detected by PCR analysis. Then, the recombinant was injected into the cortical motor area of the rats with severe spinal cord injury, and the saline was also injected into other rats with severe spinal cord injury as a match control. The functional recovery of the rats' hindlimb was assessed using BBB score and the nerve fiber of the injured region was observed by nerve tracing.
  
  
  RESULTS
  The rate of recombinant infecting rat cortical neuron in vitro exceeded 99%. PCR analysis confirmed that the effect of lentiviral vector-mediated RNA interfering gene NgR of rat cortical neurons in vitro was 61%. Although all rats with spinal cord injury were observed to have the hindlimb functional recovery, these rats injected with recombinant had better hindlimb functional recovery than others showing by more BBB score (P < 0.01). Moreover, it was found that some nerve fiber passed the injured spinal cord region of the rats which were injected with recombinant.
  
  
  CONCLUSIONS
  The recombinant lentiviral vector with siNgR199 which had been constructed is able to promote the growth of nerve fiber and the functional recovery of the rats' hindlimb.
authors.affiliation: Department of Orthopaedics, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
authors.forename: Bi-tao
authors.initials: BT
authors.lastname: Lü
authors.forename: Wen
authors.initials: W
authors.lastname: Yuan
authors.forename: Sheng-ming
authors.initials: SM
authors.lastname: Xu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins; D005822:Genetic Vectors; D006614:Hindlimb / Q000503:physiopathology; D016086:Lentivirus / Q000235:genetics; D009185:Myelin Proteins; D009416:Nerve Regeneration; D009474:Neurons; D000070816:Nogo Receptor 1; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide / Q000235:genetics*; D013119:Spinal Cord Injuries / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D058630:Spinal Cord Regeneration; D014162:Transfection
chemical_list: D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C486581:Rtn4r protein, rat