title: The response of spinal microglia to chemotherapy-evoked painful peripheral neuropathies is distinct from that evoked by traumatic nerve injuries.
abstract: Painful peripheral neuropathies produced by nerve trauma are accompanied by substantial axonal degeneration and by a response in spinal cord microglia that is characterized by hypertrophy and increased expression of several intracellular and cell-surface markers, including ionizing calcium-binding adapter molecule 1 (Iba1) and Cd11b (a complement receptor 3 antigen recognized by the OX42 antibody). The microglia response has been hypothesized to be essential for the pathogenesis of the neuropathic pain state. In contrast, the painful peripheral neuropathies produced by low doses of cancer chemotherapeutics do not produce degeneration of axons in the peripheral nerve, although they do cause partial degeneration of the sensory axons' distal-most tips, that is the intraepidermal nerve fibers that form the axons' terminal receptor arbors. The question thus arises as to whether the relatively minor and distal axonal injury characterizing the chemotherapy-evoked neuropathies is sufficient to evoke the microglial response that is seen after traumatic nerve injury. We examined the lumbar spinal cord of rats with painful peripheral neuropathies due to the anti-neoplastic agents, paclitaxel, vincristine, and oxaliplatin, and the anti-retroviral agent, 2',3'-dideoxycytidine (ddC), and compared them to rats with a complete sciatic nerve transection and the partial sciatic nerve injury produced in the chronic constriction injury model (CCI). As expected, microglia hypertrophy and increased expression of Iba1 were pronounced in the nerve transection and CCI animals. However, there was no microglia hypertrophy or increased Iba1 staining in the animals treated with paclitaxel, vincristine, oxaliplatin, or ddC. These results suggest that the mechanisms that produce neuropathic pain after exposure to chemotherapeutics may be fundamentally different than those operating after nerve trauma.
authors.affiliation: Department of Anesthesia, McGill University, Montréal, QC, Canada.
authors.forename: F Y
authors.initials: FY
authors.lastname: Zheng
authors.forename: W-H
authors.initials: WH
authors.lastname: Xiao
authors.forename: G J
authors.initials: GJ
authors.lastname: Bennett
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D019771:Axotomy; D002135:Calcium-Binding Proteins / Q000096:biosynthesis; D007150:Immunohistochemistry; D008297:Male; D008840:Microfilament Proteins; D017628:Microglia / Q000473:pathology*; D009437:Neuralgia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D010523:Peripheral Nervous System Diseases / Q000209:etiology* / Q000473:pathology* / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000473:pathology*; D013116:Spinal Cord / Q000473:pathology
chemical_list: C100745:Aif1 protein, rat; D000970:Antineoplastic Agents; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins