title: Intercellular adhesion molecule-1 plays a critical role in glomerulosclerosis after subtotal nephrectomy.
abstract: BACKGROUND
  Hyperfiltration in the glomeruli have been considered to be an important cause of glomerular injury; however, the role of intercellular adhesion molecule (ICAM)-1 in the pathogenesis of glomerulosclerosis is not known.
  
  
  METHODS
  To elucidate the effects of ICAM-1 depletion on hyperfiltration-induced glomerular disorder, we used subtotally nephrectomized ICAM-1(+/+) and ICAM-1(-/-) mice. We evaluated macrophage infiltration, mesangial matrix expansion, transforming growth factor (TGF)-β and type IV collagen accumulation in glomeruli.
  
  
  RESULTS
  Macrophage infiltration into the glomeruli and mesangial matrix expansion coincident with increased expression of both ICAM-1 and TGF-β, and accumulation of type IV collagen were ameliorated in subtotally nephrectomized ICAM-1(-/-) mice compared to ICAM-1(+/+) mice. ICAM-1 depletion significantly reduced hyperfiltration-induced glomerular injury after renal ablation.
  
  
  CONCLUSIONS
  Our present findings suggest that glomerular hyperfiltration is the leading cause of glomerulosclerosis, and it is mediated, at least in part, by ICAM-1 expression and macrophage infiltration.
authors.affiliation: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kido
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ogawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shikata
authors.forename: Motofumi
authors.initials: M
authors.lastname: Sasaki
authors.forename: Ryo
authors.initials: R
authors.lastname: Nagase
authors.forename: Shinichi
authors.initials: S
authors.lastname: Okada
authors.forename: Hitomi
authors.initials: H
authors.lastname: Usui Kataoka
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Makino
mesh_terms: D000818:Animals; D024141:Collagen Type IV / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007674:Kidney Diseases / Q000473:pathology / Q000503:physiopathology*; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009392:Nephrectomy; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
chemical_list: D024141:Collagen Type IV; D016212:Transforming Growth Factor beta; D018799:Intercellular Adhesion Molecule-1