title: Poly(o-phenylenediamine) colloid-quenched fluorescent oligonucleotide as a probe for fluorescence-enhanced nucleic acid detection.
abstract: In this Letter, we demonstrate that chemical oxidation polymerization of o-phenylenediamine (OPD) by potassium bichromate at room temperature results in the formation of submicrometer-scale poly(o-phenylenediamine) (POPD) colloids. Such colloids can absorb and quench dye-labeled single-stranded DNA (ssDNA) very effectively. In the presence of a target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the POPD surface, leading to recovery of dye fluorescence. With the use of an oligonucleotide (OND) sequence associated with human immunodeficiency virus (HIV) as a model system, we demonstrate the proof of concept that POPD colloid-quenched fluorescent OND can be used as a probe for fluorescence-enhanced nucleic acid detection with selectivity down to single-base mismatch.
authors.affiliation: State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
authors.forename: Jingqi
authors.initials: J
authors.lastname: Tian
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xuping
authors.initials: X
authors.lastname: Sun
mesh_terms: D003102:Colloids / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005453:Fluorescence; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D009841:Oligonucleotides / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry*
chemical_list: D003102:Colloids; D009841:Oligonucleotides; D010655:Phenylenediamines; C530487:poly(o-phenylenediamine); D004247:DNA