title: An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides.
abstract: We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.
authors.affiliation: Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA. liz@dbi.udel.edu
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Adams
authors.forename: Salvador
authors.initials: S
authors.lastname: Almagro-Moreno
authors.forename: E Fidelma
authors.initials: EF
authors.lastname: Boyd
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D005732:Gangliosides / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D008051:Lipid Bilayers / Q000378:metabolism*; D008322:Mammals; D018625:Microscopy, Atomic Force / Q000379:methods*; D014734:Vibrio cholerae / Q000502:physiology*
chemical_list: D005732:Gangliosides; D008051:Lipid Bilayers