title: Production of interleukin-13 is influenced by the interleukin-4 -34TT and -590TT genotype in patients with aggressive periodontitis.
abstract: Aggressive periodontitis (AgP) is a specific form of periodontal disease, with rapid destruction of the tissues supporting the teeth in otherwise young healthy individuals. We recently showed a higher frequency of the interleukin-4 (IL-4) -34TT and -590TT genotype in AgP patients compared to controls (P<0.05). Herein, we demonstrated that this specific IL-4 genotype exerts its function by increasing expression of IL-4 and STAT6, and producing higher concentrations of IL-4 in activated CD4+ cells of patients with AgP. In the present study, we investigated the effects of the IL-4-specific genotype on IL-13, IL-2 and IFN-γ expression and production in activated CD4+ cells of patients with AgP and healthy controls. Results revealed higher IFN-γ and IL-2 expression and significantly increased IL-13 production in the cells of the patients who were homozygous for the -34T and -590T alleles in comparison with the patients who were homozygous for the -34C and -590C alleles (P<0.05). Results of controls with the -34C and -590C alleles were similar to those of AgP with the same genotype. To our knowledge, the present study is the first to show an effect of the -34TT and -590TT genotype on IL-13 production. There is an increased production of IL-13 by the T cells of aggressive periodontitis patients with the IL-4 genotype.
authors.affiliation: Department of Periodontology, Justus-Liebig-University, Giessen, Germany. jose.gonzales@dentist.med.uni-giessen.de
authors.forename: J R
authors.initials: JR
authors.lastname: Gonzales
authors.forename: S
authors.initials: S
authors.lastname: Gröger
authors.forename: G
authors.initials: G
authors.lastname: Haley
authors.forename: R-H
authors.initials: RH
authors.lastname: Bödeker
authors.forename: J
authors.initials: J
authors.lastname: Meyle
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D055113:Chronic Periodontitis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D018793:Interleukin-13 / Q000096:biosynthesis / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics* / Q000276:immunology; D008297:Male
chemical_list: D018793:Interleukin-13; D007376:Interleukin-2; D015847:Interleukin-4; D007371:Interferon-gamma