title: Ischemic preconditioning of the graft for intestinal transplantation in rats.
abstract: To test the hypothesis that two modalities of IPC should decrease acute rejection and BT after SBTx in rats. Orthotopic allogenic SBTx was performed from Wistar to BN. IPC was performed by 2 ' and 5 ' superior mesenteric artery clamping, following 2-min and 5-min reperfusion before graft cooling and retrieving. Donor-recipient sets were randomly allocated to five groups: IPC2m4d, IPC2m7d, IPC5min7d, and the control groups for the two end points; ctrl4d and ctrl7d. IRI, rejection, and BT were assessed after four or seven days depending on the groups. Measured variables included: histology, leukocyte activation by tissue MPO determination, and proinflammatory cytokines (IL-b and TNF-α) to assess inflammatory response. Leukocyte activation was significantly reduced in IPC2m7d in comparison with Ctrl and IPC5min7d. Rejection tended to be lower in IPC2min7d. Cytokine levels were contradictory and not consistent with histology. Finally, BT was less frequent in IPC2min4d group but this benefit was missed in animals with rejection (7d). Inflammatory response (MPO) was reduced and rejection tended to be lower after in IPC2m7d. Bacterial translocation was reduced in IPC2min4d but the benefit was missed at day 7.
authors.affiliation: Department of Pediatric Surgery and Research Laboratory, Hospital Universitario La Paz, Madrid, Spain.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Francisco
authors.initials: F
authors.lastname: Hernandez
authors.forename: Federica
authors.initials: F
authors.lastname: Pederiva
authors.forename: Ane M
authors.initials: AM
authors.lastname: Andrés
authors.forename: Nuria
authors.initials: N
authors.lastname: Leal
authors.forename: Emilio
authors.initials: E
authors.lastname: Burgos
authors.forename: Maria P
authors.initials: MP
authors.lastname: Martínez
authors.forename: Manuel
authors.initials: M
authors.lastname: Molina
authors.forename: Manuel López
authors.initials: ML
authors.lastname: Santamaría
authors.forename: Juan A
authors.initials: JA
authors.lastname: Tovar
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D016207:Cytokines / Q000378:metabolism; D006085:Graft Survival; D007150:Immunohistochemistry / Q000379:methods; D053583:Interleukin-1beta / Q000097:blood; D007422:Intestines / Q000473:pathology / Q000637:transplantation*; D019194:Ischemic Preconditioning; D017538:Mesenteric Artery, Superior / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha