title: [Effect of acetyl-11-keto-β-boswellic acid on proliferation, apoptosis and cell cycle of human acute myeloid leukemia cell line HL-60].
abstract: The aim of this study was to investigate the effect of 3-O-acetyl-11-keto-β-boswellic acid (AKBA) on the proliferation, apoptosis and cell cycle of human acute myeloid leukemia (AML) cell line HL-60. HL-60 cells were treated by AKBA at various concentrations. The inhibitory effects of AKBA on the proliferation of HL-60 were analyzed by MTT assay. Morphologic changes of HL-60 cells were observed by fluorescence microscopy with Hochest33342 staining. Cell apoptosis rate was determined by flow cytometry with Annexin-V-FITC/PI double staining. The cell cycle was measured by flow cytometry with PI staining. The results showed that AKBA inhibited the proliferation of HL-60 and the apoptosis rate of HL-60 cells was gradually enhanced when AKBA dose increased. AKBA changed the cell cycle of HL-60, resulting in cell increase at G(1) phase and decrease at S phase. It is concluded that the AKBA has anti-proliferation and apoptosis-inducing effects on HL-60 cells, that seems a promising therapeutical approach for AML.
authors.affiliation: Department of Hematology, Central South University, Changsha 410008, Hunan Province, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yuan
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Qi
authors.forename: Min-Yuan
authors.initials: MY
authors.lastname: Peng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wan
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Wang
authors.forename: Fang-Ping
authors.initials: FP
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D014315:Triterpenes; C094432:acetyl-11-ketoboswellic acid