title: Discrimination between the regioisomeric 1,2- and 1,3-diacylglycerophosphocholines by phospholipases.
abstract: The artificial 1,3-diacyl-glycero-2-phosphocholines (1,3-PCs), which form similar aggregate structures as the naturally occurring 1,2-diacyl-sn-glycero-3-phosphocholines (1,2-PCs), were tested as substrates for different classes of phospholipases such as phospholipase A(2) (PLA(2)) from porcine pancreas, bee and snake venom, and Arabidopsis thaliana, phospholipase C (PLC) from Bacillus cereus, and phospholipase D (PLD) from cabbage and Streptomyces species. The regioisomers of the natural phospholipids were shown to bind to all investigated phospholipases with an affinity similar to the corresponding naturally occurring phospholipids, however their hydrolysis was reduced to different degrees (PLA(2)s and PLC) or even abolished (PLDs belonging to the PLD superfamily). The results are in accordance with binding models obtained by docking the substrates to the crystal structures or homology models of the phospholipases.
authors.affiliation: Institute of Biochemistry and Biotechnology, Martin-Luther University Halle-Wittenberg, Saale, Kurt-Mothes, Germany. mansfeld@biochemtech.uni-halle.de
authors.forename: Johanna
authors.initials: J
authors.lastname: Mansfeld
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brandt
authors.forename: Regine
authors.initials: R
authors.lastname: Haftendorn
authors.forename: Regina
authors.initials: R
authors.lastname: Schöps
authors.forename: Renate
authors.initials: R
authors.lastname: Ulbrich-Hofmann
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D001409:Bacillus cereus / Q000201:enzymology; D001514:Bee Venoms / Q000201:enzymology; D001516:Bees; D001665:Binding Sites; D004546:Elapid Venoms / Q000201:enzymology; D017815:Elapidae; D010179:Pancreas / Q000201:enzymology; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism*; D054467:Phospholipases A2 / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013552:Swine; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D001514:Bee Venoms; D004546:Elapid Venoms; D010713:Phosphatidylcholines; D054467:Phospholipases A2; D010738:Type C Phospholipases