title: Multiplex PCR for identification of herpes virus infections in adolescents.
abstract: The aim of the study was to develop a multiplex PCR (mPCR) for a rapid and simultaneous detection of herpes simplex 1 (HSV-1), herpes simplex 2 (HSV-2), and human cytomegalovirus (HCMV) DNA in squamous oral cells obtained from adolescents. Accuracy of the method was tested in a group of 513 adolescents, almost 11% of subjects were positive for infection with herpes viruses. Correlations with gender, age, and place of residence were sought. A similar incidence of HSV-2 and HCMV was found (4.3% and 5.4%, respectively) and the incidence of HSV-1 was the lowest (1%) in the study group. Conversely to HSV-2, HCMV was detected mostly in the youngest individuals. The same occurrence of all viruses was observed in boys and girls. The mPCR method described is suggested as a useful tool for epidemiologic studies of active herpes infections.
authors.affiliation: Faculty of Biology, Department of Molecular Virology, Adam Mickiewicz University, Poznań, Poland. juliadur@amu.edu.pl
authors.forename: Julia
authors.initials: J
authors.lastname: Durzyńska
authors.forename: Joanna
authors.initials: J
authors.lastname: Pacholska-Bogalska
authors.forename: Maria
authors.initials: M
authors.lastname: Kaczmarek
authors.forename: Tomasz
authors.initials: T
authors.lastname: Hanć
authors.forename: Magdalena
authors.initials: M
authors.lastname: Durda
authors.forename: Magdalena
authors.initials: M
authors.lastname: Skrzypczak
authors.forename: Anna
authors.initials: A
authors.lastname: Goździcka-Józefiak
mesh_terms: D000293:Adolescent; D002648:Child; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D004279:DNA, Viral / Q000235:genetics; D004847:Epithelial Cells / Q000821:virology; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis* / Q000453:epidemiology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D008297:Male; D009061:Mouth Mucosa / Q000821:virology; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral