title: Flavobacterium chungbukense sp. nov., isolated from soil.
abstract: A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100(T), was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100(T) belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100(T) showed the highest sequence similarities to Flavobacterium glaciei JCM 13953(T) (97.6 %) and Flavobacterium johnsoniae KACC 11410(T) (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5-97.0 %. Growth occurred at 4-30 °C, at pH 5.0-9.0 and in the presence of 0-2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C(15 : 0) (17.3 %), summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c, 15.5 %) and C(16 : 0) (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100(T) hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA-DNA relatedness was 3.0 % with F. glaciei JCM 13953(T) and 11.5 % with F. johnsoniae KACC 11410(T). On the basis of the evidence from this study, strain CS100(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100(T) ( = KACC 15048(T) = JCM 17386(T)).
authors.affiliation: Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: Chae-Sung
authors.initials: CS
authors.lastname: Lim
authors.affiliation: Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: Yong-Sik
authors.initials: YS
authors.lastname: Oh
authors.affiliation: Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: A-Rum
authors.initials: AR
authors.lastname: Park
authors.affiliation: School of Electrical and Computer Engineering, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: Jae-Soo
authors.initials: JS
authors.lastname: Yoo
authors.affiliation: Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
authors.affiliation: Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Roh
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S