title: [Alteration of methylation status of death-associated protein kinase (dapk) gene promoter in patients with acute myeloid leukemia].
abstract: This study was purposed to analyze the methylation status of death-associated protein kinase (dapk) gene promoter in Chinese patients with acute myeloid leukemia (AML) and its relationship with clinical features. The methylation-specific PCR (MSP) technique was used to detect dapk promoter methylation in bone marrow samples from 112 cases of AML. The results indicated that gene dapk promoter hypermethylation was detected in 82 cases (73.2%), but not in 13 control group. There was no correlation of dapk gene hypermethylation with sex, age, WBC counts, platelet counts, hematologic parameters, chromosomal abnormalities and different subtypes of AML patients. It is concluded that dapk gene hypermethylation may be a common molecular event in AML.
authors.affiliation: Department of Hematology, Jiangsu University Affiliated People Hospital, Zhenjiang 212002, Jiangsu Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Yao
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Run-Bi
authors.initials: RB
authors.lastname: Ji
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qian
authors.forename: Gao-Fei
authors.initials: GF
authors.lastname: Xiao
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation; D064550:Death-Associated Protein Kinases; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases