title: New insights into the genetic organization of the FK228 biosynthetic gene cluster in Chromobacterium violaceum no. 968.
abstract: The biosynthetic gene cluster of FK228, an FDA-approved anticancer natural product, was identified and sequenced previously. The genetic organization of this gene cluster has now been delineated through systematic gene deletion and transcriptional analysis. As a result, the gene cluster is redefined to contain 12 genes: depA through depJ, depM, and a newly identified pathway regulatory gene, depR.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, USA.
authors.forename: Vishwakanth Y
authors.initials: VY
authors.lastname: Potharla
authors.forename: Shane R
authors.initials: SR
authors.lastname: Wesener
authors.forename: Yi-Qiang
authors.initials: YQ
authors.lastname: Cheng
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis; D001483:Base Sequence; D002853:Chromatography, Liquid; D002861:Chromobacterium / Q000235:genetics*; D047630:Depsipeptides / Q000096:biosynthesis*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D013058:Mass Spectrometry; D005810:Multigene Family; D009876:Operon; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000903:Antibiotics, Antineoplastic; D047630:Depsipeptides; C087123:romidepsin