title: Accelerated angiogenic induction and vascular  integration in a novel synthetic scaffolding matrix for  tissue replacement.
abstract: OBJECTIVE
  Reduced or delayed neovascularization is a major obstacle with regard to tissue-engineered constructs. The aim of this study was to evaluate the early microvascular response to a novel degradable ε-caprolactone terpolymer matrix.
  
  
  METHODS
  ε-caprolactone terpolymer matrices (Suprathel Plus®; Institute of Textile and Process Engineering, Denkendorf, Germany) were implanted into dorsal skinfold chambers of balb/c mice (n=10). Microcirculatory changes were observed by intravital fluorescence microscopy. Scaffolding matrices from PEGT/PBT copolymer were used as controls (n=10).
  
  
  RESULTS
  The formation of de novo vascular networks within both scaffolding matrices was noted throughout the experiment. A vascular ingrowth of perfused microvessels into the matrices up to 600 μm apart from the edge was noted within 10 days of implantation. The earliest signs of neoangiogenesis were visible in ε-caprolactone terpolymer matrices on day 1. In both scaffolds the new developed vessels extended centripetally from the border of the matrices towards the center and anastomosed to form a perfused microvascular network. There was significantly earlier onset of vascularization, increased vascularized area and higher vessel density in ε-caprolactone terpolymer matrices compared to PEGT/PBT copolymer matrices were observed.
  
  
  CONCLUSIONS
  The scaffolding matrix from ε-caprolactone terpolymer allowed for an earlier and more intense induction of angiogenesis and displayed the tendency to vascularize more rapidly within a shorter period of time after transplantation compared to PEGT/PBT copolymer scaffolds, thus indicating its potential application for tissue engineering purposes.
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany. andrej.ring@ruhr-uni-bochum.de
authors.forename: Andrej
authors.initials: A
authors.lastname: Ring
authors.forename: Ole
authors.initials: O
authors.lastname: Goertz
authors.forename: Sammy
authors.initials: S
authors.lastname: Al-Benna
authors.forename: Christian
authors.initials: C
authors.lastname: Ottomann
authors.forename: Stefan
authors.initials: S
authors.lastname: Langer
authors.forename: Lars
authors.initials: L
authors.lastname: Steinstraesser
authors.forename: Inge
authors.initials: I
authors.lastname: Schmitz
authors.forename: Daniel
authors.initials: D
authors.lastname: Tilkorn
mesh_terms: D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D055806:Microvessels / Q000502:physiology* / Q000648:ultrastructure; D018919:Neovascularization, Physiologic; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012867:Skin / Q000098:blood supply*; D013499:Surface Properties; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C527789:poly(lactide-co-trimethylenecarbonate-co-epsilon-caprolactone); C072932:polyethylene oxide-polybutylene terephthalate copolymer; D011092:Polyethylene Glycols