title: Up-regulation of the vitamin C transporter SVCT2 upon differentiation and depolarization of myotubes.
abstract: In addition to its role as a strong antioxidant, vitamin C regulates the differentiation of several cell lineages. In vertebrate skeletal muscle, the vitamin C transporter SVCT2 is preferentially expressed in slow muscle fibers. To gain insights into the possible involvement of intracellular vitamin C on early myogenesis, we investigated the regulation of SVCT2 expression in cultures of chick fetal myoblasts. SVCT2 expression increases in cultures of both, slow and fast muscle-derived myoblasts, as they fuse to form mainly fast myotubes. Interestingly, we found that SVCT2 could be positively modulated by potassium-induced depolarization of myotubes. These findings suggest that SVCT2-mediated uptake of vitamin C could play diverse roles on skeletal muscle development and physiology.
authors.affiliation: Department of Cell Biology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion, Chile.
authors.forename: Marcela
authors.initials: M
authors.lastname: Low
authors.forename: Daniel
authors.initials: D
authors.lastname: Sandoval
authors.forename: Bryan
authors.initials: B
authors.lastname: Morales
authors.forename: Francisco
authors.initials: F
authors.lastname: Nualart
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Henríquez
mesh_terms: D000818:Animals; D001205:Ascorbic Acid; D002454:Cell Differentiation; D016764:Cell Polarity; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000166:cytology*; D032446:Myoblasts; D029363:Organic Anion Transporters, Sodium-Dependent / Q000096:biosynthesis*; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D029363:Organic Anion Transporters, Sodium-Dependent; D060347:Sodium-Coupled Vitamin C Transporters; D027981:Symporters; D001205:Ascorbic Acid