title: Induction of IFN-γ by a highly branched 1,3-β-d-glucan from Aureobasidium pullulans in mouse-derived splenocytes via dectin-1-independent pathways.
abstract: We have previously elucidated the precise structure of a unique type of 1,3-β-D-glucan, AP-FBG (Aureobasidium pullulans-fermented β-D-glucan), from the fungus A. pullulans and found that AP-FBG strongly induced the production of various cytokines in DBA/2 mouse-derived splenocytes in vitro. However, the mechanism(s) of action of AP-FBG on in vitro mouse primary cells have not been characterized in detail. Herein, we report that the production of IFN-γ in DBA/2 mouse-derived splenocytes by AP-FBG was not inhibited following treatment with an anti-dectin-1 neutralizing antibody. In addition, AP-FBG not only failed to activate dectin-1-mediated signaling pathways, examined by a reporter gene assay but also failed to bind to dectin-1, a pivotal receptor for 1,3-β-D-glucan. Taken together, AP-FBG induced cell activation via dectin-1-independent pathways.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Tada
authors.forename: Masashi
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Futoshi
authors.initials: F
authors.lastname: Ikeda
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kato
authors.forename: Takao
authors.initials: T
authors.lastname: Kuge
authors.forename: Asuka
authors.initials: A
authors.lastname: Tanioka
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Tsubaki
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D000818:Animals; D001203:Ascomycota; D007369:Interferon Inducers / Q000737:chemistry / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D037181:Lectins, C-Type; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology*; D000099181:Dectin-1
chemical_list: D007369:Interferon Inducers; D007371:Interferon-gamma; D037181:Lectins, C-Type; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D047071:beta-Glucans; D000099181:Dectin-1; C033363:beta-1,3-glucan