title: Evaluation of the cytokines IL-10 and IL-13 as mediators in the progression of Symmers fibrosis in patients with hepatosplenic schistosomiasis mansoni.
abstract: OBJECTIVE
  To investigate the serum levels of IL-10 and IL-13 in patients with hepatosplenic schistosomiasis mansoni (HSM), evaluating the role of these cytokines in the development of hepatic fibrosis.
  
  
  METHODS
  The study was prospective and analytical, developed at the Department of Surgery, Federal University of Pernambuco, Keizo Asami Laboratory of Immunology. We studied three groups: Group I - 25 patients with hepatosplenic schistosomiasis mansoni who were not submitted to surgery; Group II - 30 individuals who underwent splenectomy and ligature of left gastric vein; Group III - 33 subjects without hepatosplenic schistosomiasis mansoni or any other disease or condition that could compromise the hepatic functional reserve. Serum concentrations of IL-10 and IL-13 were obtained through ELISA. Considering their non-parametric nature, all concentrations were analyzed by Kruskal-Wallis test, with p<0.05 used to reject the null hypothesis.
  
  
  RESULTS
  The mean concentrations of IL-10 in ng/mL in serum were GI: 50.0 ± 59.0; GII: 38.0 ± 270; GIII: 38.0 ± 20.0. Concentrations of IL-13 in ng/mL in the serum of patients were respectively: 41.0 ± 93.0 in GI, 16.0 ± 17.0 in GII and 18.0 ± 34.0 in GIII. There was no significant difference between the mean concentrations of IL-10 and IL-13 between the study groups (p> 0.05).
  
  
  CONCLUSIONS
  The mean serum concentrations of IL-10 and IL-13 were similar in all three groups, indicating that possibly the presence of these cytokines in serum is not associated with different degrees of Symmers fibrosis in patients with hepatosplenic schistosomiasis mansoni.
authors.affiliation: Department of Pediatric Surgery, Hospital das Clinicas of the Center for Health Sciences and at the Keizo Assam Immunopathology Laboratory (LIKA) - Federal University of Pernambuco, Recife, Brazil. carlosbrandt@bol.com.br
authors.forename: Carlos Teixeira
authors.initials: CT
authors.lastname: Brandt
authors.forename: Mario
authors.initials: M
authors.lastname: Rino
authors.forename: Maira Galdino da Rocha
authors.initials: MG
authors.lastname: Pitta
authors.forename: Janinne Siqueira
authors.initials: JS
authors.lastname: Muniz
authors.forename: Diego de Oliveira
authors.initials: Dde O
authors.lastname: Silveira
authors.forename: Célia Maria Machado Barbosa de
authors.initials: CM
authors.lastname: Castro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D018450:Disease Progression; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D018793:Interleukin-13 / Q000097:blood*; D008103:Liver Cirrhosis / Q000097:blood* / Q000469:parasitology* / Q000601:surgery; D008109:Liver Diseases, Parasitic / Q000097:blood* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012555:Schistosomiasis mansoni / Q000097:blood* / Q000601:surgery; D013158:Splenic Diseases / Q000097:blood* / Q000469:parasitology* / Q000601:surgery; D055815:Young Adult
chemical_list: D018793:Interleukin-13; D016753:Interleukin-10