title: Anti-mycobacterial activity of garlic (Allium sativum) against multi-drug resistant and non-multi-drug resistant mycobacterium tuberculosis.
abstract: Emergence of multi-drug resistant (MDR) and extensively drug resistant (XDR) TB throughout the developing world is very disturbing in the present scenario of TB management. There is a fundamental need to explore alternative anti-TB agents. Hence natural plants should be investigated to understand their antimicrobial properties and safety. Garlic (Allium sativum) is one of natural plant which possesses variety of biological properties like anti-tumor, anti-hyperlipedemic and anti-microbial etc. The present study was evaluated for anti-bacterial activity of garlic against non-MDR and MDR isolates of M. tuberculosis. A total of 20 clinical isolates of MTB including 15 MDR and 5 non-MDR were investigated. Ethanolic extract of garlic was prepared by maceration method. Minimum inhibitory concentration (MIC) was performed by using 7H9 middle brook broth dilution technique. MIC of garlic extract was ranged from 1 to 3 mg/ml; showing inhibitory effects of garlic against both non-MDR and MDR M. tuberculosis isolates. Alternate medicine practices with plant extracts including garlic should be considered to decrease the burden of drug resistance and cost in the management of diseases. The use of garlic against MDR-TB may be of great importance regarding public health.
authors.affiliation: Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Hannan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ikram Ullah
authors.forename: Muhammad
authors.initials: M
authors.lastname: Usman
authors.forename: Shahid
authors.initials: S
authors.lastname: Hussain
authors.forename: Muhammad
authors.initials: M
authors.lastname: Absar
authors.forename: Khursheed
authors.initials: K
authors.lastname: Javed
mesh_terms: D000995:Antitubercular Agents; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D024901:Drug Resistance, Multiple, Bacterial; D000431:Ethanol; D005737:Garlic / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology; D011786:Quality Control; D012997:Solvents
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; D004121:Dimethyl Sulfoxide