title: Multigenic system controlling viral systemic infection determined by the interactions between Cucumber mosaic virus genes and quantitative trait loci of soybean cultivars.
abstract: Soybean 'Harosoy' is resistant to Cucumber mosaic virus soybean strain C (CMV-SC) and susceptible to CMV-S strain D (CMV-SD). Using enzyme-linked immunosorbent assay and Northern hybridization, we characterized the Harosoy resistance and found that CMV-SC did not spread systemically but was restricted to the inoculated leaves in Harosoy. Harosoy resistance was not controlled by either a dominant or recessive single gene. To dissect this system controlling long-distance movement of CMV in soybean, we constructed infectious cDNA clones of CMV-SC and CMV-SD. Using these constructs and the chimeric RNAs, we demonstrated that two viral components were required for systemic infection by the virus. The region including the entire 2b gene and the 5' region of RNA3 (mainly the 5' untranslated region) together were required. By quantitative trait locus (QTL) analysis using an F(2) population and the F(3) families derived from Harosoy and susceptible 'Nemashirazu', we also showed that at least three QTLs affected systemic infection of CMV in soybean. Our study on Harosoy resistance to CMV-SC revealed an interesting mechanism, in which multiple host and viral genes coordinately controlled viral systemic infection.
authors.affiliation: Hokkaido University, Sapporo, Japan.
authors.forename: Shizen
authors.initials: S
authors.lastname: Ohnishi
authors.forename: Issei
authors.initials: I
authors.lastname: Echizenya
authors.forename: Eri
authors.initials: E
authors.lastname: Yoshimoto
authors.forename: Kim
authors.initials: K
authors.lastname: Boumin
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inukai
authors.forename: Chikara
authors.initials: C
authors.lastname: Masuta
mesh_terms: D002678:Chimera; D002874:Chromosome Mapping; D017799:Cucumovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D017343:Genes, Plant / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D018515:Plant Leaves / Q000235:genetics / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000235:genetics; D011523:Protoplasts / Q000821:virology; D040641:Quantitative Trait Loci; D012367:RNA, Viral / Q000235:genetics / Q000502:physiology; D016865:Reassortant Viruses / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D013025:Glycine max / Q000235:genetics* / Q000821:virology*
chemical_list: D018076:DNA, Complementary; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral