title: Effect of alpha-ketoglutarate on neurobehavioral, neurochemical and oxidative changes caused by sub-chronic cyanide poisoning in rats.
abstract: Recent studies revealed that alpha-ketoglutarate (A-KG) alone or with sodium thiosulfate (STS) provide significant protection against acute and sub-acute cyanide poisoning in rodents. This study addresses the protective effect of A-KG and/or STS in sub-chronic (90 days) cyanide poisoning. Wistar rats were divided into seven groups (n = 10): Control animals, potassium cyanide (KCN) A-KG, STS, KCN + A-KG, KCN + STS and KCN + A-KG + STS. Spontaneous motor activity and motor coordination were recorded every 15th day. Lipid peroxidation (LPO), reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) in blood, brain, liver and kidney, and glutamate, aspartate and dopamine in discrete regions of brain were measured following 90 days exposure. Cyanide significantly decreased motor coordination, accompanied by increase in LPO (blood, brain and liver) and dopamine (corpus striatum and cerebral cortex) levels, and depletion in GSH (blood, brain and liver), GPx (brain and liver), SOD (brain and liver), and CAT (blood and brain) levels. Although treatment of A-KG and STS alone significantly blunted the toxicity of KCN, concomitant use of both afforded the maximum protection. This study shows a promising role of A-KG and STS as treatment regime for long term cyanide exposure.
authors.affiliation: Department of Physiology, Chettinad Hospital & Research Institute, Kelambakkam, Tamil Nadu, 603 103, India. mathangidc@hotmail.com
authors.forename: D C
authors.initials: DC
authors.lastname: Mathangi
authors.forename: R
authors.initials: R
authors.lastname: Shyamala
authors.forename: R
authors.initials: R
authors.lastname: Vijayashree
authors.forename: K R
authors.initials: KR
authors.lastname: Rao
authors.forename: A
authors.initials: A
authors.lastname: Ruckmani
authors.forename: R
authors.initials: R
authors.lastname: Vijayaraghavan
authors.forename: R
authors.initials: R
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D003486:Cyanides / Q000506:poisoning*; D004298:Dopamine / Q000378:metabolism; D006801:Humans; D007656:Ketoglutaric Acids / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013885:Thiosulfates / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D003486:Cyanides; D007656:Ketoglutaric Acids; D018696:Neuroprotective Agents; D013885:Thiosulfates; C017717:sodium thiosulfate; D004298:Dopamine