title: IL-6 SNP diversity among four ethnic groups as revealed by bead-based liquid array profiling.
abstract: In the present work, we established a rapid, cost-effective and high-throughput method for genotyping using a multiplexed microsphere-based suspension array platform - Luminex(®) ×MAP™, which enabled us to analyse two SNPs in the promoter of IL-6 gene, determining haplotypes associated with different levels of expression. Using this system, IL-6 diversity in four different ethnic groups - East Asians, Caucasians, Hispanic and African Americans - was assessed. Results showed a significant variability in terms of allele, genotype and haplotype distribution. Considering the important immunoregulatory role of this cytokine and as a clinically relevant marker, this genotyping approach will provide a powerful tool for disease association or transplant outcome studies.
authors.affiliation: Central Laboratory of Clinical Immunology, University Hospital Alexandrovska, Sofia, Bulgaria. mivanova@intech.bg
authors.forename: M
authors.initials: M
authors.lastname: Ivanova
authors.forename: J
authors.initials: J
authors.lastname: Ruiqing
authors.forename: S
authors.initials: S
authors.lastname: Kawai
authors.forename: M
authors.initials: M
authors.lastname: Matsushita
authors.forename: N
authors.initials: N
authors.lastname: Ochiai
authors.forename: E
authors.initials: E
authors.lastname: Maruya
authors.forename: H
authors.initials: H
authors.lastname: Saji
mesh_terms: D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D005820:Genetic Testing; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D015850:Interleukin-6