title: Modulation of ionising radiation generated oxidative stress by HI-6 (asoxime) in a laboratory rat model.
abstract: OBJECTIVE
  HI-6 is an antidotum suitable for treatment of intoxication by nerve agents. The recent investigation appointed its modulation of inflammatory response as well as vegetative nervous system activity. However, the present experiments were carried out in order to assess the antioxidant effect of HI-6 in irradiated animals.
  
  
  METHODS
  male Wistar rats were irradiated by ionizing radiation (7.5 Gy, LD50/30). Animals were divided into four groups: i.e. controls (A), irradiated (B), treated with HI-6 (C), and both irradiated and treated with HI-6 (D). Ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS) and glutathione reductase activity were assayed in liver, spleen, plasma, and whole blood. Clinical biochemistry markers were determined in plasma samples.
  
  
  RESULTS
  We found significantly increased FRAP levels in liver, while its levels decreased in the spleen of B group animals. Ionising radiation (B group) also significantly elevated TBARS values in spleen. HI-6 reversed FRAP and TBARS values to control levels. Glutathione reductase activity was significantly elevated in spleen and liver of animals exposed to HI-6 (C and D groups). Clinical biochemistry markers were shifted only slightly. The in vitro test confirmed the inhibitory effect of HI-6 towards acetylcholinesterase.
  
  
  CONCLUSIONS
  In conclusion, HI-6 is potent in suppressing oxidative stress and might be a promising drug in the field of radiation protection.
authors.affiliation: Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. miroslav.pohanka@gmail.com
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pohanka
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Pejchal
authors.forename: Svatava
authors.initials: S
authors.lastname: Horackova
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
authors.forename: Hana
authors.initials: H
authors.lastname: Bandouchova
authors.forename: Veronika
authors.initials: V
authors.lastname: Damkova
authors.forename: Jiri
authors.initials: J
authors.lastname: Pikula
mesh_terms: D000818:Animals; D000931:Antidotes / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D005980:Glutathione Reductase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D010091:Oximes / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D011839:Radiation, Ionizing; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000931:Antidotes; D000975:Antioxidants; D010091:Oximes; D011726:Pyridinium Compounds; D017392:Thiobarbituric Acid Reactive Substances; D005980:Glutathione Reductase; C022870:asoxime chloride