title: A novel binding protein of single immunoglobulin IL-1 receptor-related molecule: Paralemmin-3.
abstract: Previous studies have shown that single immunoglobulin IL-1 receptor-related molecule (SIGIRR) is a negative regulator of Toll-Interleukin-1 receptor signaling. Nevertheless, the molecular mechanism of the negatively regulatory effect of SIGIRR remains unknown. Using a yeast two-hybrid screen, we identified paralemmin-3 (PALM3) as a novel binding protein of SIGIRR. This interaction of SIGIRR with PALM3 was confirmed by coimmunoprecipitation in mammalian cells. In addition, the PALM3 mRNA expression was upregulated by lipopolysaccharide (LPS)-stimulation in a human alveolar epithelial cell line (A549 cells). Furthermore, silencing PALM3 by RNA interference inhibited the release of inflammatory cytokines in A549 cells after LPS-stimulation. These results suggest that PALM3 may function as an adaptor in the LPS- Toll-like receptor 4 signaling and the interaction of SIGIRR with PALM3 may partly account for the mechanism of the negatively regulatory effect of SIGIRR.
authors.affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, PR China.
authors.forename: Xuxin
authors.initials: X
authors.lastname: Chen
authors.forename: Xueling
authors.initials: X
authors.lastname: Wu
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guansong
authors.initials: G
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Guisheng
authors.initials: G
authors.lastname: Qian
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008070:Lipopolysaccharides / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034622:RNA Interference; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D016207:Cytokines; D008070:Lipopolysaccharides; C556449:PALM3 protein, human; D010750:Phosphoproteins; D017472:Receptors, Interleukin-1; C546891:SIGIRR protein, human; D051197:Toll-Like Receptor 4