title: Histidine domain-protein tyrosine phosphatase interacts with Grb2 and GrpL.
abstract: BACKGROUND
  Histidine domain-protein tyrosine phosphatase (HD-PTP) plays a key role in vesicle trafficking and biogenesis. Although it is a large protein with at least five distinct structural domains, only a few of its interactors are presently known, and the significance of these interactions is largely obscure.
  
  
  RESULTS
  In this study we performed a yeast two-hybrid screening using a human colon cDNA library and found that Grb2 and GrpL are binding partners of HD-PTP. Co-immunoprecipitation, pull-down and immunocytochemistry experiments confirmed the interactions. We also discovered that the central proline-rich and histidine-rich domain of HD-PTP is responsible for these interactions.
  
  
  CONCLUSIONS
  The interaction of HD-PTP with two adapters of the Grb2 family, essential for numerous signaling pathways, suggests that HD-PTP might be important for signaling through a plethora of receptors.
authors.affiliation: Department of Enzymology, Institute of Biochemistry, Bucharest, Romania. ctanase@biochim.ro
authors.forename: Carmen-Alexandra
authors.initials: CA
authors.lastname: Tanase
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D003106:Colon / Q000378:metabolism; D051380:GRB2 Adaptor Protein / Q000737:chemistry / Q000378:metabolism*; D015723:Gene Library; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D054558:Protein Tyrosine Phosphatases, Non-Receptor / Q000737:chemistry*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C409587:GRAP2 protein, human; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D006639:Histidine; D011392:Proline; C114102:PTPN23 protein, human; D054558:Protein Tyrosine Phosphatases, Non-Receptor