title: Substrate inhibition in human hydroxysteroid sulfotransferase SULT2A1: studies on the formation of catalytically non-productive enzyme complexes.
abstract: The cytosolic sulfotransferase hSULT2A1 is the major hydroxysteroid (alcohol) sulfotransferase in human liver, and it catalyzes the 3'-phosphoadenosine-5'-phosphosulfate (PAPS)-dependent sulfation of various endogenous hydroxysteroids as well as many xenobiotics that contain alcohol and phenol functional groups. The hSULT2A1 often displays substrate inhibition, and we have hypothesized that a key element in this response to increasing substrate concentration is the formation of non-productive ternary dead-end enzyme complexes involving the nucleotide product, adenosine 3',5'-diphosphate (PAP). One of these substrates for hSULT2A1 is dehydroepiandrosterone (DHEA), a major circulating steroid hormone in humans that serves as precursor to both androgens and estrogens. We have utilized DHEA in both initial velocity studies and equilibrium binding experiments in order to evaluate the potential role of ternary complexes in substrate inhibition of the enzyme. Our results indicate that hSULT2A1 forms non-productive ternary complexes that involve either DHEA or dehydroepiandrosterone sulfate, and the formation of these ternary complexes displays negative cooperativity in the binding of DHEA.
authors.affiliation: Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Hayrettin Ozan
authors.initials: HO
authors.lastname: Gulcan
authors.forename: Michael W
authors.initials: MW
authors.lastname: Duffel
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism / Q000494:pharmacology; D055162:Biocatalysis; D003687:Dehydroepiandrosterone / Q000378:metabolism / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D013451:Sulfonic Acids / Q000378:metabolism / Q000494:pharmacology; D015238:Sulfotransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D013451:Sulfonic Acids; D003687:Dehydroepiandrosterone; D010724:Phosphoadenosine Phosphosulfate; D000244:Adenosine Diphosphate; D015238:Sulfotransferases; C028152:alcohol sulfotransferase