title: Methods for using Galleria mellonella as a model host to study fungal pathogenesis.
abstract: The facile inoculum delivery and handling of the insect Galleria mellonella make it a desirable model for the study of fungal pathogenesis. Here we present methods to study fungal virulence, filamentation and fungal cell associates with insect hemocytes using Candida albicans and Cryptococcus neoformans to illustrate the use of this model. The two types of fungi cause distinct infections thus we compare and contrast the infection characteristics observed in G. mellonella. The protocols presented herein can be adapted to the study of other fungal pathogens using G. mellonella as an infection model.
authors.affiliation: Division of Infectious, Massachusetts General Hospital, Boston, MA, USA. hfuchs@partners.org
authors.forename: Beth Burgwyn
authors.initials: BB
authors.lastname: Fuchs
authors.forename: Elizabeth
authors.initials: E
authors.lastname: O'Brien
authors.forename: Joseph B El
authors.initials: JB
authors.lastname: Khoury
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Mylonakis
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D003455:Cryptococcus neoformans / Q000472:pathogenicity*; D006434:Hemocytes / Q000382:microbiology*; D009036:Moths / Q000382:microbiology*; D013534:Survival; D014774:Virulence