title: Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP9) gene in the reproduction seasons of Chinese mitten crab, Eriocheir sinensis.
abstract: Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional requirements during periods of reproductive activity. Therefore, a cDNA encoding Eriocheir sinensis FABP (Es-FABP9) was cloned based upon EST analysis of a testis cDNA library. The full length cDNA was 898 bp and encoded a 136 aa polypeptide that was highly homologous to related genes reported in shrimp. Gene expression analysis, as determined by RT-PCR, revealed the presence of Es-FABP9 transcripts was widely distributed with high and detectable expression levels observed in intestine, ovary, testis and heart, while expression were comparable among hepatopancreas, hemolymphe, gills, muscle, stomach and brain. Real-time quantitative RT-PCR analysis revealed that Es-FABP9 expression in testis, hemolymphe, hepatopancreas and ovarian was dependent on the status of testis development. Evidence provided in the present report supports a role of Es-FABP9 in lipid transport during the period of rapid testis growth in E. sinensis, and indirectly confirms the participation of the hepatopancreas, testis, hemolymphe and ovarian in lipid nutrient absorption and utilization processes.
authors.affiliation: School of Life Science, East China Normal University, North Zhong-Shan Road, Shanghai 200062, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Xing-Kun
authors.initials: XK
authors.lastname: Jin
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Gong
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics*; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics*; D010802:Phylogeny; D012098:Reproduction / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins