title: High survival of frozen cells irradiated with gamma radiation.
abstract: Cell storage in liquid nitrogen (LN) offers the most secure method of cell preservation even if cryopreserved cells are exposed to natural background of ionising radiation (IR). A lot of experiments have demonstrated that IR can induce damages in living cells, but only a little information regarding the response of cryopreserved cells is available. To investigate the effect of IR on frozen and unfrozen cells, peripheral blood mononuclear cells were directly irradiated at room temperature, then immediately frozen, or frozen and then irradiated in LN with different doses of gamma rays. After thawing, cells were incubated and death fraction was evaluated at different time points. Interestingly, the percentages of dead cells induced by IR gradually increased with both dose radiation and incubation time and were significantly lower for cells irradiated at -196°C than those irradiated at room temperature.
authors.affiliation: Department of Human, Environmental and Natural Sciences, University of Urbino Carlo Bo, via Ca Le Suore, 2/4, 61029 Urbino (PU), Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Cugia
authors.forename: Filippo
authors.initials: F
authors.lastname: Centis
authors.forename: Genny
authors.initials: G
authors.lastname: Del Zotto
authors.forename: Alessandra
authors.initials: A
authors.lastname: Lucarini
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Argazzi
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Zini
authors.forename: Massimo
authors.initials: M
authors.lastname: Valentini
authors.forename: Monica
authors.initials: M
authors.lastname: Bono
authors.forename: Francesco
authors.initials: F
authors.lastname: Picardi
authors.forename: Stefania
authors.initials: S
authors.lastname: Stramigioli
authors.forename: Werther
authors.initials: W
authors.lastname: Cesarini
authors.forename: Loris
authors.initials: L
authors.lastname: Zamai
mesh_terms: D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured; D015925:Cryopreservation; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D008297:Male; D011829:Radiation Dosage