title: Antimicrobial efficacy of chemical disinfectants on contaminated full metal crowns.
abstract: Prosthetic restorations that have been tried in the patient's mouth are potential sources of infection. In order to avoid cross-infection, protocols for infection control should be established in dental office and laboratory. This study evaluated the antimicrobial efficacy of disinfectants on full metal crowns contaminated with microorganisms. Full crowns cast in a Ni-Cr alloy were assigned to one control group (n=6) and 5 experimental groups (n=18). The crowns were placed in flat-bottom glass balloons and were autoclaved. A microbial suspension of each type of strain - S. aureus, P. aeruginosa, S. mutans, E. faecalis and C. albicans- was aseptically added to each experimental group, the crowns being allowed for contamination during 30 min. The contaminated specimens were placed into recipients with the chemical disinfectants (1% and 2% sodium hypochlorite and 2% glutaraldehyde) for 5, 10 and 15 min. Thereafter, the crowns were placed into tubes containing different broths and incubated at 35ºC. The control specimens were contaminated, immersed in distilled water for 20 min and cultured in Thioglycollate broth at 35ºC. Microbial growth assay was performed by qualitative visual examination after 48 h, 7 and 12 days. Microbial growth was noticed only in the control group. In the experimental groups, turbidity of the broths was not observed, regardless of the strains and immersion intervals, thus indicating absence of microbial growth. In conclusion, all chemical disinfectants were effective in preventing microbial growth onto full metal crowns.
authors.affiliation: Department of Dental Materials and Prosthodontics, University of São Paulo, Ribeirão Preto, SP, Brazil. iaraorsi@forp.usp.br
authors.forename: Iara Augusta
authors.initials: IA
authors.lastname: Orsi
authors.forename: Camilo Andrés
authors.initials: CA
authors.lastname: Villabona
authors.forename: Eliana
authors.initials: E
authors.lastname: Kameoka
authors.forename: Marly Christiènne C G
authors.initials: MC
authors.lastname: Ferreira
authors.forename: Izabel Yoko
authors.initials: IY
authors.lastname: Ito
authors.forename: Marta Estela
authors.initials: ME
authors.lastname: Saravia
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D003428:Cross Infection / Q000517:prevention & control; D003442:Crowns / Q000382:microbiology*; D003666:Decontamination / Q000379:methods*; D003722:Dental Alloys; D019551:Dental Disinfectants / Q000737:chemistry*; D004866:Equipment Contamination; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D003722:Dental Alloys; D019551:Dental Disinfectants; D012973:Sodium Hypochlorite; D005976:Glutaral