title: Acceleration of keratinocyte differentiation by transient receptor potential vanilloid (TRPV6) channel activation.
abstract: BACKGROUND
  Numerous studies have demonstrated the beneficial effect of Avène Thermal Spring Water (TSW) in dermatological diseases but the molecular mechanisms remain unknown. The objective of the present study was to evaluate the effect of Avène TSW on the morphological and molecular features related to the more advanced status of differentiation of human keratinocytes.
  
  
  METHODS
  Normal human keratinocytes (NHK) were differentiated in medium powder reconstituted with Avène TSW and assessed by RT-PCR and immunohistochemistry. Calcium entry was measured by a Fura-2 AM probe. TRPV6 channel were detected by immunohistochemistry, RT-PCR and western blot.
  
  
  RESULTS
  Treatment of NHK with Avène TSW led to an enhanced constitutive calcium entry that resulted in the increased expression of involucrin and cytokeratins 1 and 10. This enhanced constitutive calcium entry in Avène TSW-treated keratinocytes was mediated by the TRPV6 calcium channel. Moreover, Avène TSW-mediated calcium entry was due to the increase in TRPV6 expression as well as the channel abundance at the cell membrane.
  
  
  CONCLUSIONS
  An other mechanism of action of Avène TSW is described. Avène TSW treatment induced an enhanced constitutive calcium entry mediated by TRPV6 channel leading to the acceleration of human keratinocytes differentiation.
authors.affiliation: Inserm, U-1003, Equipe labellisée par Ligue Nationale contre cancer, Université de Lille-1 (USTL), Villeneuve d'Ascq, France.
authors.forename: V
authors.initials: V
authors.lastname: Lehen'kyi
authors.forename: M
authors.initials: M
authors.lastname: Vandenberghe
authors.forename: F
authors.initials: F
authors.lastname: Belaubre
authors.forename: S
authors.initials: S
authors.lastname: Julié
authors.forename: N
authors.initials: N
authors.lastname: Castex-Rizzi
authors.forename: R
authors.initials: R
authors.lastname: Skryma
authors.forename: N
authors.initials: N
authors.lastname: Prevarskaya
mesh_terms: D002118:Calcium; D020013:Calcium Signaling / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D053556:Keratin-1 / Q000235:genetics / Q000378:metabolism; D053550:Keratin-10 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000378:metabolism; D008900:Mineral Waters / Q000008:administration & dosage*; D011498:Protein Precursors / Q000378:metabolism; D050916:TRPV Cation Channels / Q000187:drug effects* / Q000378:metabolism
chemical_list: D053556:Keratin-1; D026901:Membrane Transport Proteins; D008900:Mineral Waters; D011498:Protein Precursors; D050916:TRPV Cation Channels; C120776:TRPV6 channel; D053550:Keratin-10; C032253:involucrin; D002118:Calcium