title: Size, concentration and incubation time dependence of gold nanoparticle uptake into pancreas cancer cells and its future application to X-Ray Drug Delivery System.
abstract: One of the restrictions in the potential use of gold markers for medical imaging/tracking of harder tumors is its size. We propose to use gold nanoparticles which, due to its small size, can be administered conveniently via intravenous injection. One of the factors that determine the clinical utility of nanoparticles is the ability to enter cells. In this report, the stability of gold nanoparticles mixed with different media was determined by UV-vis spectroscopy. Gold nanoparticle size was confirmed by TEM. Intracellular uptake using different gold nanoparticle sizes, incubation times and concentrations were analyzed using Atomic Absorption Spectrometry (AAS). Temperature dependence uptake was also measured using AAS. The results showed that pancreas cancer cells uptake 20 nm gold nanoparticles preferentially compared to other gold nanoparticle sizes. Efficient accumulation of gold nanoparticles into pancreas cancer cells can be achieved at longer incubation time and higher concentration. The findings of this study will help in the design and optimization of the gold nanoparticle-based agents for therapeutic and diagnostic applications of X-ray Drug Delivery System.
authors.affiliation: Department of Nuclear Engineering and Management, The University of Tokyo, Tokyo, Japan. trono@nuclear.jp
authors.forename: Jade Dungao
authors.initials: JD
authors.lastname: Trono
authors.forename: Kazue
authors.initials: K
authors.lastname: Mizuno
authors.forename: Noritaka
authors.initials: N
authors.lastname: Yusa
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsukawa
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Uesaka
mesh_terms: D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D004058:Diffusion; D016503:Drug Delivery Systems / Q000379:methods / Q000639:trends; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000000981:diagnostic imaging*; D010316:Particle Size; D011859:Radiography
chemical_list: D003287:Contrast Media; D053769:Nanocapsules; D006046:Gold