title: Label-free detection of polynucleotide single-base mismatch via pyrene probe excimer emission.
abstract: The pyrene probe and pyrene-labeled oligonucleotides (ODNs) probe are expected to be candidates as fluorescent probe for DNA assay. In particular, label-free detection is a very hot because of its simpleness, speediness and cheapness. Herein, we have investigated the use of a pyrenylakylammonium salt, a novel fluorescent probe for the detection of one single nucleotide polymorphism (SNP) in double stranded DNA. After S1 nuclease digestion, the pyrene probes bind electrostatically to the perfect complement DNA and emit a strong excimer emission. However, treatment of the non-complementary DNA with S1 nuclease caused nucleotide fragments of less than 5 bases, which could not induce excimer emission. By comparing ratio of excimer to monomer fluorescence between normal and mutant DNA after S1 nuclease digestion, One-base mutation in DNA was detected easily. This new method may be applied to the detection of SNP.
authors.affiliation: Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tang
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Dongli
authors.initials: D
authors.lastname: Liao
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Yang
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D020137:Base Pair Mismatch / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D011119:Polynucleotides / Q000235:genetics*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D018076:DNA, Complementary; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D011119:Polynucleotides; D011721:Pyrenes; C030984:pyrene; D003851:Deoxyribonucleases