title: A large set of estrogen receptor β-interacting proteins identified by tandem affinity purification in hormone-responsive human breast cancer cell nuclei.
abstract: Estrogen receptors α (ER-α) and β (ER-β) play distinct biological roles in onset and progression of hormone-responsive breast cancer, with ER-β exerting a modulatory activity on ER-α-mediated estrogen signaling and stimulation of cell proliferation by mechanisms still not fully understood. We stably expressed human ER-β fused to a tandem affinity purification-tag in estrogen-responsive MCF-7 cells and applied tandem affinity purification and nanoLC-MS/MS to identify the ER-β interactome of this cell type. Functional annotation by bioinformatics analyses of the 303 proteins that co-purify with ER-β from nuclear extracts identify several new molecular partners of this receptor subtype that represents nodal points of a large protein network controlling multiple processes and functions in breast cancer cells.
authors.affiliation: Department of General Pathology, Second University of Naples, Napoli, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Nassa
authors.forename: Roberta
authors.initials: R
authors.lastname: Tarallo
authors.forename: Concetta
authors.initials: C
authors.lastname: Ambrosino
authors.forename: Angela
authors.initials: A
authors.lastname: Bamundo
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Ferraro
authors.forename: Ornella
authors.initials: O
authors.lastname: Paris
authors.forename: Maria
authors.initials: M
authors.lastname: Ravo
authors.forename: Pietro H
authors.initials: PH
authors.lastname: Guzzi
authors.forename: Mario
authors.initials: M
authors.lastname: Cannataro
authors.forename: Marc
authors.initials: M
authors.lastname: Baumann
authors.forename: Tuula A
authors.initials: TA
authors.lastname: Nyman
authors.forename: Ernesto
authors.initials: E
authors.lastname: Nola
authors.forename: Alessandro
authors.initials: A
authors.lastname: Weisz
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism*; D002846:Chromatography, Affinity; D047629:Estrogen Receptor beta / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans
chemical_list: D047629:Estrogen Receptor beta; D004967:Estrogens