title: Glia-induced reversible disruption of blood-brain barrier integrity and neuropathological response of the neurovascular unit.
abstract: The blood-brain barrier (BBB) is the regulated interface that mediates selective transcellular transport of nutrients and essential components from the blood into the brain parenchyma. Many neurodegenerative diseases including stroke, multiple sclerosis, rheumatoid arthritis, and AIDS dementia exhibit loss of BBB integrity. Despite the increasing body of evidence for the involvement of glia in maintaining the BBB, few studies have addressed glial/endothelial/extracellular matrix interactions. A chemically induced astrocyte lesion provides a noninvasive model to study reversible BBB dysfunction in vivo. Blood-brain barrier integrity was assessed with fluorescent dextran tracers (3-70 kDa) and magnetic resonance imaging, in parallel with confocal and electron microscopy imaging of the neurovascular unit. These studies demonstrated modified tight-junction protein expression with loss of vascular integrity. We propose that adherens junction proteins and extracellular matrix remodeling provide a temporary size-selective barrier, whereas astrocyte and microglia activation direct tight-junction proteins to paracellular domains and restore BBB integrity. Morphological comparisons were made with the area postrema, a circumventricular organ with a naturally porous BBB. Further studies into cellular mechanisms of glial/endothelial/extracellular matrix interactions may identify novel glial-based therapeutic targets and innovate therapies for modulating diseases in which gliosis and raised levels of pro-inflammatory mediators are central components.
authors.affiliation: Department of Biomedical Sciences, College of Osteopathic Medicine and Center for Excellence in the Neurosciences, University of New England, Biddeford, ME 04005, USA. cwillis1@une.edu
authors.forename: Colin L
authors.initials: CL
authors.lastname: Willis
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D031608:Area Postrema / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000378:metabolism* / Q000473:pathology*; D001921:Brain / Q000098:blood supply / Q000378:metabolism; D057167:Claudins / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D023421:Models, Animal; D009422:Nervous System Diseases / Q000473:pathology; D009457:Neuroglia / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D019108:Tight Junctions / Q000378:metabolism; D057900:Transcytosis
chemical_list: D057167:Claudins