title: Spectroscopic investigation on protein damage by ciprofloxacin under ultrasonic irradiation.
abstract: In recent years, sonodynamic activities of many drugs have attracted more and more attention of researchers. The correlative study will promote the development of sonodynamic therapy (SDT) in anti-tumor treatment. In this work, bovine serum albumin (BSA) was used as a protein model to investigate the intensifying effects of ciprofloxacin (CPFX) ultrasonically induced protein damage by UV-vis and fluorescence spectra. Meanwhile, the conformation of BSA is changed upon the addition of CPFX and metal ions under ultrasound (US) so that the damaging site of BSA is considered. Various influencing factors, such as US irradiation time, metal ions, solution temperature and ionic strength, on the ultrasonically induced BSA damage are discussed. It was showed that CPFX could enhance ultrasonically induced BSA damage. The damage degree of BSA was aggravated with the increasing of US irradiation time, solution temperature, ionic strength as well as the addition of metal ions. Furthermore, the reactive oxygen species (ROS) in reaction system were detected by oxidation-extraction photometry (OEP). Experimental results also showed that US could activate CPFX to produce ROS, which were mainly determined as superoxide radical anion (.O2-) and hydroxyl radical (.OH).
authors.affiliation: Department of Chemistry, Northeastern University, Shenyang 110819, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Dong-Jing
authors.initials: DJ
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Bing-Mi
authors.initials: BM
authors.lastname: Liu
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Kong
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Xu
mesh_terms: D000042:Absorption / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D002417:Cattle; D002939:Ciprofloxacin / Q000737:chemistry / Q000494:pharmacology*; D008670:Metals; D009994:Osmolar Concentration; D011487:Protein Conformation / Q000187:drug effects / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000187:drug effects* / Q000528:radiation effects*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics / Q000295:instrumentation / Q000379:methods*; D014966:Xanthenes / Q000737:chemistry
chemical_list: D008670:Metals; D017382:Reactive Oxygen Species; D012996:Solutions; D014966:Xanthenes; D012710:Serum Albumin, Bovine; D002939:Ciprofloxacin; C049068:3',4'-deoxypsorospermin-4'-chloro-3'-ol