title: Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for the enterocyte effacement (LEE) gene.
abstract: The effects of detergents (cholic acid, deoxycholic acid, Triton X-100, and Nonidet P-40) on the secretion of EspB from the locus for enterocyte effacement (LEE) gene-positive Escherichia coli strains were examined. Clinical isolates of eight EPEC strains and seven STEC strains were used to detect EspB after they had been cultivated in Luria-Bertani (LB) broth containing one of the detergents. When the bacteria were cultured in LB broth supplemented with one of the detergents, the amount of EspB produced was increased by 2-32-fold depending on the detergent and the strain used. EspB was detected in all strains when they were cultured in LB broth containing all of the detergents. The results obtained in this study can be applied to immunological diagnostic methods for detecting EspB and also to the production of EspB for research purposes.
authors.affiliation: Department of Molecular Bacteriology and Immunology, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan. noboru@med.u-ryukyu.ac.jp
authors.forename: Noboru
authors.initials: N
authors.lastname: Nakasone
authors.forename: Claudia
authors.initials: C
authors.lastname: Toma
authors.forename: Naomi
authors.initials: N
authors.lastname: Higa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Koizumi
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ogura
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D003902:Detergents / Q000378:metabolism*; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006801:Humans; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D015533:Transcriptional Activation
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D003902:Detergents; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins