title: [ERK1/2 mediates edaravone-triggered protection against myocardial damage induced by isoprenaline in H9c2 cells].
abstract: OBJECTIVE
  To explore the effect of extracellular signal regulated kinase 1/2 (ERK1/2) on edaravone (EDA)-triggered protection against myocardial toxicity induced by isoprenaline (ISO) in H9c2 myocardial cells (H9c2 cells).
  
  
  METHODS
  H9c2 cells were exposed to ISO at different concentrations to establish a cardiac toxicity model induced by persistent excitation of β1 receptor. EDA was added before ISO as a pretreatment. PD-98059, an ERK1/2 inhibitor, was administered 1 h prior to EDA to inhibit the phosphorylation of ERK1/2. Cell viability was measured using cell counter kit (CCK-8). The expressions of p-ERK1/2 and t-ERK1/2 were tested by Western blotting. Mitochondrial membrane potential (MMP) was detected by Rhodamine123 (Rh123) staining and photofluorography.
  
  
  RESULTS
  Exposure of H9c2 cells to 80 µmol/L ISO for 24 h down-regulated ERK1/2 phosphorylation and repressed MMP. Pretreatment with 10-40 µmol/L EDA for 1 h inhibited ISO-induced myocardial toxicity and pretreatment of 40 µmol/L EDA partially rescued ERK1/2 phosphorylation and MMP level. PD-98059 abolished cardiac protection of EDA, leading to myocardial toxicity and MMP loss.
  
  
  CONCLUSIONS
  EDA can protect H9c2 cells against myocardial injury induced by ISO by suppressing ISO-triggered inhibition of ERK1/2 activation.
authors.affiliation: Department of Internal Medicine, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiu-yu
authors.initials: XY
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Fu
authors.forename: Chun-tao
authors.initials: CT
authors.lastname: Yang
authors.forename: Li-qiu
authors.initials: LQ
authors.lastname: Mo
authors.forename: Zhan-li
authors.initials: ZL
authors.lastname: Yang
authors.forename: Xiao-bian
authors.initials: XB
authors.lastname: Dong
authors.forename: Xin-xue
authors.initials: XX
authors.lastname: Liao
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Feng
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D000077553:Edaravone; D005419:Flavonoids / Q000494:pharmacology; D007545:Isoproterenol / Q000633:toxicity; D020935:MAP Kinase Signaling System; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats
chemical_list: D005419:Flavonoids; D048052:Mitogen-Activated Protein Kinase 3; D007545:Isoproterenol; D000077553:Edaravone; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D000983:Antipyrine