title: [Analysis the relationship of HBV BCP A1762T/G1764A double mutation with HBV related acute on chronic liver failure].
abstract: OBJECTIVE
  To analysis the relationship between HBV BCP A1762T/G1764A double mutation with acute on chronic liver failure (ACLF).
  
  
  METHODS
  HBV BCP A1762T/G1764A double mutation was detected in 166 HBV chronic infection patients by nested PCR and direct DNA sequencing. The mutation rate was compared among the patients with different disease course.
  
  
  RESULTS
  Among 166 patients, 45 patients, 45 patients, 49 patients and 27 patients were diagnosed as chronic hepatitis B (CHB), liver cirrhosis (LC), ACLF and hepatocellular carcinoma (HCC), respectively. A1762T/G1764A double mutation rate was 40.0% (18/45), 84.4% (38/45), 73.5% (36/49) and 92.6% (25/27) respectively in different groups. However, A1762T/G1764A double mutation rate has no difference between ACLF based on CHB and LC (P = 0.502) and between patients with HBeAg positive and negative (P = 0.735). HBV DNA level (log) of patients with A1762T/G1764A double mutation was 5.68 +/- 1.36, lower than but having no significant statistic difference compared to patients without the double mutation (6.14 +/- 1.81, P = 0.075).
  
  
  CONCLUSIONS
  A1762T/G1764A double mutation has a close relationship with the progress of HBV-infection diseases, but is not specific to patients with ACLF. And patients with BCP double mutation have similar HBV DNA levels and HBeAg status with patients without the double mutation.
authors.affiliation: Liver Failure Treatment and Research Center, 302 Hospital, Beijing 100039, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Su
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Zang
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wang
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D058625:End Stage Liver Disease / Q000235:genetics; D005260:Female; D005838:Genotype; D006513:Hepatitis B e Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic; D006801:Humans; D017114:Liver Failure, Acute / Q000235:genetics*; D008297:Male; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D013223:Statistics as Topic; D014758:Viral Core Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D006513:Hepatitis B e Antigens; D014758:Viral Core Proteins