title: Behaviour of mesenchymal stem cells, fibroblasts and osteoblasts on smooth surfaces.
abstract: Understanding of the interactions between cells and surfaces is essential in the field of tissue engineering and biomaterials. This study aimed to compare the adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs), an osteoblast cell line (MC3T3-E1) and gingival fibroblasts (HGF-1) on tissue culture polystyrene (TCPS), glass and titanium (Ti). The average surface roughness was 5, 0.2 and 40×10(-3) μm for TCPS, glass and Ti, respectively. Immunocytochemistry and image analysis made it possible to quantify the number and morphology of adherent cells as well as the density of the focal points. Regardless of the substrate, both hMSCs and osteoblastic cells were mainly branch-shaped. HGF-1 exhibited a significantly higher number of focal points on Ti than on TCPS and glass. Alizarin red quantification indicated that both hMSCs and osteoblastic cells were more differentiated on TCPS than on Ti and glass. The surface properties of substrates, such as roughness, wettability and chemical composition, modulated the behaviour of the cells. Early events, such as cell adhesion, may influence the differentiation of hMSC and consequently tissue healing around implanted biomaterials.
authors.affiliation: Inserm U957, Bone Resorption Physiopathology and Primary Bone Tumor Therapy, Faculty of Medicine, University of Nantes, Nantes, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lavenus
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Guy
authors.initials: G
authors.lastname: Louarn
authors.forename: Pierre
authors.initials: P
authors.lastname: Layrolle
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D005898:Glass / Q000737:chemistry*; D006801:Humans; D021621:Imaging, Three-Dimensional; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D056951:Photoelectron Spectroscopy; D011137:Polystyrenes / Q000494:pharmacology*; D013194:Staining and Labeling; D055672:Static Electricity; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000880:Anthraquinones; D011137:Polystyrenes; C010078:alizarin; D014025:Titanium; D000469:Alkaline Phosphatase