title: [Autophagy is involved in 6-OHDA-induced dopaminergic cell death].
abstract: OBJECTIVE
  To study the role of autophagy in the death of dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA).
  
  
  METHODS
  Rat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay.
  
  
  RESULTS
  Under TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group.
  
  
  CONCLUSIONS
  The increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.
authors.affiliation: Yancheng Health Vocational and Technical College, China. xialiping1976@163.com
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Xia
authors.forename: Ling-yun
authors.initials: LY
authors.lastname: Li
authors.forename: Xi-feng
authors.initials: XF
authors.lastname: Fei
authors.forename: Zhong-qin
authors.initials: ZQ
authors.lastname: Liang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000187:drug effects
chemical_list: D008869:Microtubule-Associated Proteins; D016627:Oxidopamine; C526109:Anxa3 protein, rat