title: Loss of cofilin 1 disturbs actin dynamics, adhesion between enveloping and deep cell layers and cell movements during gastrulation in zebrafish.
abstract: During gastrulation, cohesive migration drives associated cell layers to the completion of epiboly in zebrafish. The association of different layers relies on E-cadherin based cellular junctions, whose stability can be affected by actin turnover. Here, we examined the effect of malfunctioning actin turnover on the epibolic movement by knocking down an actin depolymerizing factor, cofilin 1, using antisense morpholino oligos (MO). Knockdown of cfl1 interfered with epibolic movement of deep cell layer (DEL) but not in the enveloping layer (EVL) and the defect could be specifically rescued by overexpression of cfl1. It appeared that the uncoordinated movements of DEL and EVL were regulated by the differential expression of cfl1 in the DEL, but not EVL as shown by in situ hybridization. The dissociation of DEL and EVL was further evident by the loss of adhesion between layers by using transmission electronic and confocal microscopy analyses. cfl1 morphants also exhibited abnormal convergent extension, cellular migration and actin filaments, but not involution of hypoblast. The cfl1 MO-induced cell migration defect was found to be cell-autonomous in cell transplantation assays. These results suggest that proper actin turnover mediated by Cfl1 is essential for adhesion between DEL and EVL and cell movements during gastrulation in zebrafish.
authors.affiliation: Institute of Zoology, National Taiwan University, Taipei, Taiwan, Republic of China.
authors.forename: Chun-Wei
authors.initials: CW
authors.lastname: Lin
authors.forename: Shuo-Ting
authors.initials: ST
authors.lastname: Yen
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Chang
authors.forename: Shiang-Jiuun
authors.initials: SJ
authors.lastname: Chen
authors.forename: Shih-Lei
authors.initials: SL
authors.lastname: Lai
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Liu
authors.forename: Tun-Hao
authors.initials: TH
authors.lastname: Chan
authors.forename: Wen-Lian
authors.initials: WL
authors.lastname: Liao
authors.forename: Shyh-Jye
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D051338:Cofilin 1 / Q000378:metabolism*; D005775:Gastrula / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D018613:Microscopy, Confocal / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008954:Models, Biological; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish
chemical_list: D000199:Actins; D015820:Cadherins; D051338:Cofilin 1