title: Detection of two different influenza A viruses using a nitrocellulose membrane and a magnetic biosensor.
abstract: Here we describe a new analytical method for the detection of two influenza A viruses by nitrocellulose membrane and magnetic sensors that employ a special frequency mixing technique. The combination of the nitrocellulose membrane and magnetic bead detection permits a rapid assay procedure and excludes two steps (the development of color and the stop reaction) required for usual immunochemical detection methods such as ELISA. Quantitative virus detection was performed using magnetic beads conjugated with secondary antibody. The results were compared with conventional assay methods and with a dot-blot assay with fluorescence compound (FITC). Under optimum conditions, our new assay procedure is capable of detecting picograms of virus per well. This new method combining the nitrocellulose membrane and magnetic bead detection reduces analytical time and allows stable and repeatable analyses of samples in point-of-care applications.
authors.affiliation: Electronics and Telecommunications Research Institute, Daejeon, Republic of Korea. hb8868@etri.re.kr
authors.forename: Hyo-Bong
authors.initials: HB
authors.lastname: Hong
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Krause
authors.forename: Ki-Bong
authors.initials: KB
authors.lastname: Song
authors.forename: Chel-Jong
authors.initials: CJ
authors.lastname: Choi
authors.forename: Myung-Ae
authors.initials: MA
authors.lastname: Chung
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Son
authors.forename: Andreas
authors.initials: A
authors.lastname: Offenhäusser
mesh_terms: D000914:Antibodies, Viral; D015374:Biosensing Techniques / Q000379:methods*; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology / Q000302:isolation & purification; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D008280:Magnetics; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D005456:Fluorescent Dyes; D003101:Collodion; D016650:Fluorescein-5-isothiocyanate