title: Histological development of human testicular cords from 70 to 90 days of gestation.
abstract: The development of the testicular cord structure was investigated in 4 human fetuses between 70 and 90 days of gestation, in which the testicular cords are differentiating into the seminiferous tubules. Histological examinations were performed using stains with haematoxylin-eosin (HE), Masson's trichrome (MT), periodic acid schiff (PAS), anti-proliferating cell nuclear antigen (PCNA) monoclonal antibodies, and TUNEL methods. It was found that the testicular cords structures were indefinitely observed in HE-stained sections of four fetuses. However, the basement membranes of the testicular cord were clearly stained with MT, showing the tubular structure. Furthermore, cells in the testicular cords were positive with PAS, but the interstitial tissues outside the testicular cords were negative. PCNA-positive cells were detected not only inside but also outside the testicular cords, however, TUNEL positive cells are not detected throughout all testicular tissues.
authors.affiliation: Department of Anatomy, Tokyo Medical University, Tokyo, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kurihara
authors.forename: Ning
authors.initials: N
authors.lastname: Qu
authors.forename: Baik Hwan
authors.initials: BH
authors.lastname: Cho
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Shuang-Qin
authors.initials: SQ
authors.lastname: Yi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriyama
authors.forename: Hanno
authors.initials: H
authors.lastname: Steinke
authors.forename: Steinke
authors.initials: S
authors.lastname: Hanno
authors.forename: Gen
authors.initials: G
authors.lastname: Murakami
authors.forename: Masahiro
authors.initials: M
authors.lastname: Itoh
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D005865:Gestational Age; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012671:Seminiferous Tubules / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D013085:Spermatic Cord / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D018809:Proliferating Cell Nuclear Antigen