title: [Dynamics of calcium in the hippocampal neuronal culture model of epilepsy].
abstract: OBJECTIVE
  To observe the intracellular calcium ion spatio-temporal and dynamic changes in the hippocampal neuronal culture model of epilepsy induced by low magnesium ion medium, and to explore the relationship between calcium ion and epilepsy.
  
  
  METHODS
  Applying both laser scanning confocal microscope and patch clamp to timely observe the changes of [Ca2+]i and electrophysiological in the hippocampal neuronal culture model of epilepsy, and the influence of NMDA receptor-gated channels retarder and non-NMDA receptor-gated channels retarder.
  
  
  RESULTS
  After the hippocampal nerve cell broken into epileptiform discharges, [(Ca2+]i rapidly ascended to (620 +/- 70) nmol/L, NMIDA acceptor retarder (MK-801, 10 micromol/L) and non-NMDA acceptor retarder (NBQX, 10 micromol/L) reduced [Ca2+]i ascendance. Recovery of the elevated [Ca2+]i was obviously delay, after 90 min and 150 min epileptiform discharges, it took (114.8 +/- 5.2) min and (135.0 +/- 22.7) min (P < 0.05) respectively.
  
  
  CONCLUSIONS
  In vitro status epilepticus causes sustained elevation of intracellular calcium levels in hippocampal neurons
authors.affiliation: Department of Neurology, Second Affilited Hosptial, Zhejiang University College of Medicine, Hangzhou 310009, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Mei-ping
authors.initials: MP
authors.lastname: Ding
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Li
authors.forename: Fu-you
authors.initials: FY
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000378:metabolism* / Q000503:physiopathology*
chemical_list: D002118:Calcium