title: Antiangiogenic potential of three triterpenic acids in human liver cancer cells.
abstract: Three triterpenic acids, oleanolic acid, ursolic acid and maslinic acid, at 2 or 4 μmol/L were used to study their antiangiogenic potential in human liver cancer Hep3B, Huh7 and HA22T cell lines. The effects of these compounds upon the level and/or expression of hypoxia-inducible factor (HIF)-1α, basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), interleukin (IL)-8, urokinase plasminogen activator (uPA), reactive oxygen species (ROS), nitric oxide (NO) and cell invasion and migration were examined. Results showed that these triterpenic acids at 4 μmol/L significantly suppressed HIF-1α expression in three cell lines (P < 0.05); and these compounds at test doses failed to affect bFGF expression (P > 0.05). Three triterpenic acids dose-dependently decreased production and expression of VEGF and IL-8, retained glutathione level, lowered ROS and NO levels, and declined cell invasion and migration in test cell lines (P < 0.05). These compounds also dose-dependently reduced uPA production and expression in Hep3B and Huh7 cell lines (P < 0.05); but these agents only at 4 μmol/L significantly suppressed uPA production and expression in HA22T cells (P < 0.05). These findings suggest that these triterpenic acids are potent antiangiogenic agents to retard invasion and migration in liver cancer cells.
authors.affiliation: Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung City, Taiwan.
authors.forename: Chun-Che
authors.initials: CC
authors.lastname: Lin
authors.forename: Chun-Yin
authors.initials: CY
authors.lastname: Huang
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Mong
authors.forename: Chien-Yi
authors.initials: CY
authors.lastname: Chan
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Yin
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D009828:Oleanolic Acid / Q000494:pharmacology* / Q000031:analogs & derivatives; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D020533:Angiogenesis Inhibitors; D016222:Fibroblast Growth Factor 2; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D016209:Interleukin-8; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid; C412811:maslinic acid