title: [Effect of sodium valproate on human myelodysplastic syndrome cell line SKM-1 and its mechanism].
abstract: This study was aimed to investigate the effect of sodium valproate(VPA) on human myelodysplastic syndrome cell line SKM-1 and its mechanism. The cell proliferation was determined by MTT assay, cell apoptosis was analyzed by flow cytometry. The expressions of c-flipl, c-flips and dlk1 mRNA were detected by RT-PCR. The results showed that VPA could inhibited the growth of SKM-1 cells in dose- and time-dependent manners. The flow cytometric analysis indicated that VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner. The expressions of c-flipl, c-flips and dlk1 mRNA in SKM-1 cell treated with VPA decreased using of VPA. It is concluded that VPA can induce apoptosis and inhibited proliferation of SKM-1 cells. In this process, the decreasing of c-flipl, c-flips and dlk1 mRNA expression may play important roles.
authors.affiliation: Department of Hematology, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China. cba8888@hotmail.com
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Chun-Rui
authors.initials: CR
authors.lastname: Li
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhou
authors.forename: Xue-Qiong
authors.initials: XQ
authors.lastname: Wu
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Xia
authors.forename: Ze-Ye
authors.initials: ZY
authors.lastname: Shao
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Ding
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Song
authors.forename: Wen
authors.initials: W
authors.lastname: Bao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000378:metabolism; D002135:Calcium-Binding Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009190:Myelodysplastic Syndromes / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D014635:Valproic Acid