title: Evaluation of CHROMagar™ KPC for the detection of carbapenemase-producing Enterobacteriaceae in rectal surveillance cultures.
abstract: In this study, the performance of the chromogenic medium CHROMagar™ KPC was evaluated and was compared with in-house-daily prepared McConkey agar plates supplemented with imipenem (1 mg/L) for the detection of carbapenemase-producing Enterobacteriaceae. In this surveillance study, rectal swabs were cultured on both media and polymerase chain reaction (PCR) for bla(KPC) and bla(VIM) was used to confirm the genotype of growing colonies of Enterobacteriaceae. CHROMagar KPC was also tested with 17 genotypically characterised carbapenemase-producing and non-producing Gram-negative bacteria. It was shown that CHROMagar allows rapid detection of carbapenemase-producing Enterobacteriaceae, although bla(KPC)- and bla(VIM)-harbouring isolates could not be differentiated by colour or colony morphology. The positive and negative predictive values of the tested methods for the detection of carbapenemase-producing Enterobacteriaceae were, respectively, 100% and 98.8% for CHROMagar KPC and 94.7% and 88.6% for imipenem-supplemented McConkey agar. CHROMagar KPC medium is a useful screening medium for carbapenemase-producing Enterobacteriaceae in stools in settings with a high proportion of patients colonised with a variety of carbapenemase-producers.
authors.affiliation: 4th Department of Internal Medicine, Athens University School of Medicine, University General Hospital ATTIKON, Chaidari, Greece. fpanagea@yahoo.gr
authors.forename: Theofano
authors.initials: T
authors.lastname: Panagea
authors.forename: Irene
authors.initials: I
authors.lastname: Galani
authors.forename: Maria
authors.initials: M
authors.lastname: Souli
authors.forename: Panagiota
authors.initials: P
authors.lastname: Adamou
authors.forename: Anastasia
authors.initials: A
authors.lastname: Antoniadou
authors.forename: Helen
authors.initials: H
authors.lastname: Giamarellou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D003116:Color; D003470:Culture Media / Q000737:chemistry*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012007:Rectum / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase