title: The role of nitric oxide in convulsions induced by lindane in rats.
abstract: Lindane is an organochloride pesticide and scabicide. It evokes convulsions mainly trough the blockage of GABA(A) receptors. Nitric oxide (NO), gaseous neurotransmitter, has contradictor role in epileptogenesis due to opposite effects of L-arginine, precursor of NO syntheses (NOS), and L-NAME (NOS inhibitor) observed in different epilepsy models. The aim of the current study was to determine the effects of NO on the behavioral and EEG characteristics of lindane-induced epilepsy in male Wistar albino rats. The administration of L-arginine (600, 800 and 1000 mg/kg, i.p.) in dose-dependent manner significantly increased convulsion incidence and severity and shortened latency time to first convulsion elicited by lower lindane dose (4 mg/kg, i.p.). On the contrary, pretreatment with L-NAME (500, 700 and 900 mg/kg, i.p.) decreased convulsion incidence and severity and prolonged latency time to convulsion following injection with a convulsive dose of lindane (8 mg/kg, i.p.). EEG analyses showed increase of number and duration of ictal periods in EEG of rats receiving l-arginine prior to lindane and decrease of this number in rats pretreated with L-NAME. These results support the conclusion that NO plays a role of endogenous convulsant in rat model of lindane seizures.
authors.affiliation: Laboratory of Neurophysiology, Institute of Medical Physiology Richard Burian, School of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Hrnčić
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rašić-Marković
authors.forename: Dragan
authors.initials: D
authors.lastname: Djuric
authors.forename: Veselinka
authors.initials: V
authors.lastname: Sušić
authors.forename: Olivera
authors.initials: O
authors.lastname: Stanojlović
mesh_terms: D000818:Animals; D001120:Arginine / Q000633:toxicity; D001522:Behavior, Animal / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D010575:Pesticides / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology*
chemical_list: D010575:Pesticides; D009569:Nitric Oxide; D001556:Hexachlorocyclohexane; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester