title: Nuclear activity in Apis mellifera L. (Hymenoptera, Apidae) queen ovary cells demonstrated by silver nitrate impregnation and ultrastructure.
abstract: The present paper shows through conventional light microscopy, silver nitrate impregnation and Transmission Electron Microscopy, the changes in nuclear activity that occurs in the ovary cells during vitellogenesis of Apis mellifera (Linnaeus, 1758). The material impregnated by silver nitrate was detected in nurse cells, oocyte germinal vesicle and follicular cells, whose amounts are greater in phases corresponding to ovarian cells more intense activity. The silver impregnation permitted to demonstrate that the oocyte germinal vesicle synthezises ribonucleoproteins (RNP) during the beginning of the vitellogenesis. The ultrastructure show nucleolar-like material corresponding to nuclear sites impregnated by silver.
authors.affiliation: Departamento de Biologia, Instituto de Biociências de Rio Claro, Universidade Estadual Paulista, SP, Brazil. cclandim@rc.unesp.br
authors.forename: C
authors.initials: C
authors.lastname: Cruz-Landim
authors.forename: K
authors.initials: K
authors.lastname: Patrício
mesh_terms: D000818:Animals; D001516:Bees / Q000502:physiology / Q000648:ultrastructure*; D002466:Cell Nucleolus / Q000648:ultrastructure*; D005260:Female; D046529:Microscopy, Electron, Transmission; D010053:Ovary / Q000502:physiology / Q000648:ultrastructure*; D012835:Silver Nitrate; D016622:Silver Staining; D014818:Vitellogenesis / Q000502:physiology*
chemical_list: D012835:Silver Nitrate