title: Cullin5 reduces retinal cell death induced by glutamate toxicity.
abstract: OBJECTIVE
  To investigate the expression in the mouse retina and the function in a retinal cell of cullin5 that is known as a protein that is associated with ubiquitilation.
  
  
  METHODS
  The retinal sites of expressing cullin5 were determined by immunohistochemistry with a specific antibody for cullin5. Retinal cells were transfected with HA-tagged cullin5 using an adenovirus system, and retinal cells were exposed to L-glutamate with or without an over-expression of cullin5.
  
  
  RESULTS
  Cullin5 was expressed in the retina of C57BL/6N mice and the retinal ganglion cell layer (RGL) of retina was strongly immunostained. Further, cullin5 was localized in the retinal ganglion cell (RGC). An over-expression of cullin5 reduced the percentage of retinal cell deaths induced by L-glutamate.
  
  
  CONCLUSIONS
  The expression of cullin5 on retinal cells and reduction in the percentage of dead retinal cells induced by L-glutamate suggest that cullin5 has neuroprotective properties in retinal cells.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, Japan. tkana@hiroshima-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Okumichi
authors.forename: Ulfah
authors.initials: U
authors.lastname: Rimayanti
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kiuchi
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D002470:Cell Survival / Q000502:physiology; D044842:Cullin Proteins / Q000502:physiology*; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors; D018698:Glutamic Acid / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D018696:Neuroprotective Agents; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000378:metabolism; D014162:Transfection
chemical_list: D044842:Cullin Proteins; D018696:Neuroprotective Agents; C531145:cullin5 protein, mouse; D018698:Glutamic Acid