title: [Effect of ICAM-1/LFA-1 on hypoxia/reoxygenation injury of cardiomyocytes mediated by neutrophils].
abstract: OBJECTIVE
  To observe the effect of hypoxia/reoxygenation on the adherence of neutrophils to cardiomyocytes and to investigate the effect of ICAM-1/LFA-1 on hypoxia/reoxygenation injury of cardiomyocytes mediated by neutrophils.
  
  
  METHODS
  Count adhered neutrophils to cardiomyocytes suffering hypoxia, hypoxia/reoxygenation and normal culture, as well as adhered neutrophils that were blocked by anti ICAM-1 and LFA-1 monoclonal antibodies. Release of lactate dehydrogenase (LDH) was determined as injury index of cardiomyocytes.
  
  
  RESULTS
  Adherence of with normal culture group (P < 0.01). The neutrophils to cardiomyocytes with hypoxia/reoxygenation were significantly increased compared lease of LDH by cardiomyocytes was also significantly increased (P < 0.01). There was no significant difference between hypoxia group and normal control (P > 0.05). The adherence of neutrophils to hypoxia/reoxygenation cardiomyocytes was significantly inhibited by anti-ICAM-1 and anti-LFA-1 antibody compared with normal culture group (P < 0.01). While release of LDH markedly decreased (P < 0.01).
  
  
  CONCLUSIONS
  Hypoxia/reoxygenation increased the adherence of neutrophils to cardiomyocytes. ICAM-1 and LFA-1 mediated the enhanced adherence of neutrophils to hypoxia/reoxygenation cardiomyocytes. Anti-ICAM-1 and anti-LFA-1 antibody attenuated the cytotoxic effects of neutrophils on hypoxia/reoxygenated cardiomyocytes. These results suggested that the damage of neutrophils on cardiomyocytes is partly mediated by ICAM-1/LFA-1. Anti ICAM-1 and LFA-1 antibody are both beneficial in protecting hypoxia/reoxygenation cardiomyocytes from injury mediated by neutrophils.
authors.affiliation: Department of Cardiovasology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Shen
authors.forename: X L
authors.initials: XL
authors.lastname: Lang
authors.forename: T H
authors.initials: TH
authors.lastname: Zhang
authors.forename: Y W
authors.initials: YW
authors.lastname: Qin
authors.forename: G Y
authors.initials: GY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1