title: Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola).
abstract: The Arctic springtail, Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola), is one of the few organisms known to survive the extreme stresses of its environment by using cryoprotective dehydration. We have undertaken a proteomics study comparing M. arctica, acclimated at -2°C, the temperature known to induce the production of the anhydroprotectant trehalose in this species, and -6°C, the temperature at which trehalose expression plateaus, against control animals acclimated at +5°C. Using difference gel electrophoresis, and liquid chromatography tandem mass spectrometry, we identified three categories of differentially expressed proteins with specific functions, up-regulated in both the -2°C and -6°C animals, that were involved in metabolism, membrane transport and protein folding. Proteins involved in cytoskeleton organisation were only up-regulated in the -6°C animals.
authors.affiliation: British Antarctic Survey, Natural Environment Research Council, Cambridge, UK. mior@bas.ac.uk
authors.forename: M A S
authors.initials: MA
authors.lastname: Thorne
authors.forename: M R
authors.initials: MR
authors.lastname: Worland
authors.forename: R
authors.initials: R
authors.lastname: Feret
authors.forename: M J
authors.initials: MJ
authors.lastname: Deery
authors.forename: K S
authors.initials: KS
authors.lastname: Lilley
authors.forename: M S
authors.initials: MS
authors.lastname: Clark
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D000818:Animals; D001110:Arctic Regions; D003080:Cold Temperature; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007313:Insecta / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D014199:Trehalose / Q000096:biosynthesis* / Q000235:genetics; D014867:Water / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D014867:Water; D014199:Trehalose