title: The role of Arabidopsis Rubisco activase in jasmonate-induced leaf senescence.
abstract: Leaf senescence, as the last stage of leaf development, is regulated by diverse developmental and environmental factors. Jasmonates (JAs) have been shown to induce leaf senescence in several plant species; however, the molecular mechanism for JA-induced leaf senescence remains unknown. In this study, proteomic, genetic, and physiological approaches were used to reveal the molecular basis of JA-induced leaf senescence in Arabidopsis (Arabidopsis thaliana). We identified 35 coronatine-insensitive 1 (COI1)-dependent JA-regulated proteins using two-dimensional difference gel electrophoresis in Arabidopsis. Among these 35 proteins, Rubisco activase (RCA) was a COI1-dependent JA-repressed protein. We found that RCA was down-regulated at the levels of transcript and protein abundance by JA in a COI1-dependent manner. We further found that loss of RCA led to typical senescence-associated features and that the COI1-dependent JA repression of RCA played an important role in JA-induced leaf senescence.
authors.affiliation: MOE Key Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Shan
authors.forename: Junxia
authors.initials: J
authors.lastname: Wang
authors.forename: Lingling
authors.initials: L
authors.lastname: Chua
authors.forename: Dean
authors.initials: D
authors.lastname: Jiang
authors.forename: Wen
authors.initials: W
authors.lastname: Peng
authors.forename: Daoxin
authors.initials: D
authors.lastname: Xie
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D040901:Proteomics; D018749:RNA, Plant / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D054883:Oxylipins; D018749:RNA, Plant; C545839:Rca protein, Arabidopsis; C011006:jasmonic acid