title: Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging.
abstract: Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging.
authors.affiliation: Huazhong University of Science and Technology,, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
authors.forename: Tingwei
authors.initials: T
authors.lastname: Quan
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Huang
mesh_terms: D000669:Amplifiers, Electronic; D017076:Computer-Aided Design; D004579:Electron Transport; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008670:Metals / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D010087:Oxides / Q000737:chemistry; D010783:Photometry / Q000295:instrumentation*; D012666:Semiconductors; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D055672:Static Electricity
chemical_list: D008670:Metals; D010087:Oxides