title: Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus.
abstract: The present study evaluated the antimicrobial activity of three root canal irrigants against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus. These microorganisms were incubated in the presence of citric acid (6 and 10%), EDTA (17%), and NaOCl (0.5, 1.0, 2.5, and 5.25%). Agar diffusion tests were performed and redox indicator resazurin was used to evaluate the inhibitory effect of the irrigants on the metabolic activity of these microorganisms. The mean diameters of the inhibition zones for the C. albicans cultures were 11.6 mm (17% EDTA), 5.5 mm (0.5% NaOCl), 12.9 mm (1% NaOCl), 22.1 mm (2.5% NaOCl), and 28.5 mm (5.25% NaOCl). The mean diameters of the inhibition zones for E. faecalis were 2.8 mm (1% NaOCl), 5.4 mm (2.5% NaOCl), and 8.3 mm (5.25% NaOCl). For S. aureus, the mean values were 8.0 mm (17% EDTA), 3.0 mm (1% NaOCl), 8.8 mm (2.5% NaOCl), and 10.0 mm (5.25% NaOCl). Most of the irrigant solutions presented effective antimicrobial activity against C. albicans. A high inhibitory effect on the metabolic activity of E. faecalis was detected when the microorganisms were incubated with 17% EDTA. The same result was reached when S. aureus was incubated in the presence of > 2.5% NaOCl. Altogether, these results indicate that 2.5% and 5.25% NaOCl are microbicides against S. aureus while 0.5% and 1% NaOCl are only microbiostatic against the tested bacteria. The 6% and 10% citric acid as well as 17% EDTA did not affect the viability of any of the assayed microorganisms.
authors.affiliation: Federal University of Rio de Janeiro, RJ, Brazil. tatiana_kelly@yahoo.com.br
authors.forename: Tatiana Kelly da Silva
authors.initials: TK
authors.lastname: Fidalgo
authors.forename: Roberta
authors.initials: R
authors.lastname: Barcelos
authors.forename: Maristela Barbosa
authors.initials: MB
authors.lastname: Portela
authors.forename: Rosangela Maria de Araújo
authors.initials: RM
authors.lastname: Soares
authors.forename: Rogério
authors.initials: R
authors.lastname: Gleiser
authors.forename: Fernando Costa e
authors.initials: FC
authors.lastname: Silva-Filho
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D019343:Citric Acid / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid; D012973:Sodium Hypochlorite