title: N-(2-mercaptopropionyl)-glycine but not allopurinol prevented cigarette smoke-induced alveolar enlargement in mouse.
abstract: We investigated the possible protective effects of the Allopurinol (A), N-(2-mercaptopropionyl)-glycine (M) and N-acetylcysteine (N) against lung injury caused by long-term exposure to cigarette smoke (CS) in mouse. C57BL6 mice were exposed to 12 cigarettes a day for 60 days and concomitantly treated with either one of the antioxidant drugs diluted in saline (CS+A-50 mg/kg; CS+M-200 mg/kg/day; CS+N-200 mg/kg/day). Control groups were sham-smoked (AA). Long-term CS exposure results in extensive parenchyma destruction in CS group. Both CS+N and CS+M groups showed preserved alveolar structure and showed preserved lung function when compared to CS group. Macrophage and neutrophil counts were decreased in CS+M, and CS+N groups when compared to CS group (p<0.05). Antioxidant enzyme activities were reduced in all treated groups. CS+A showed the highest reduction in catalase activity (-25%, p<0.01). We conclude that M treatment reduced long-term CS-induced inflammatory lung parenchyma destruction and lung function, comparable to N treatment, however, antioxidant administration did not reverse CS-induced antioxidant enzyme activity reduction.
authors.affiliation: Laboratory of Tissue and Repair, Histology and Embryology Department, Roberto Alcantara Gomes Institute of Biology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: Karla Maria Pereira
authors.initials: KM
authors.lastname: Pires
authors.forename: Frank Silva
authors.initials: FS
authors.lastname: Bezerra
authors.forename: Mariana Nascimento
authors.initials: MN
authors.lastname: Machado
authors.forename: Walter Araújo
authors.initials: WA
authors.lastname: Zin
authors.forename: Luís Cristóvão
authors.initials: LC
authors.lastname: Porto
authors.forename: Samuel Santos
authors.initials: SS
authors.lastname: Valença
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004195:Disease Models, Animal; D004646:Emphysema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D055370:Lung Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000473:pathology; D012907:Smoking; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; C110658:N-(2-mercaptoproprionyl)-glycine; D013438:Sulfhydryl Compounds; D014028:Tobacco Smoke Pollution; D000493:Allopurinol; D005998:Glycine; D000111:Acetylcysteine