title: [Effects of PKC activation on apoptosis during ischemia/reperfusion in L-6TG rat skeletal myoblasts].
abstract: OBJECTIVE
  To study the effects of PKC activation on apoptosis during ischemia/reperfusion in L-6TG rat skeletal myoblasts.
  
  
  METHODS
  Cultured L-6TG cells were divided into 3 groups: control group (C), ischemia/reperfusion group (I/R), PMA + ischemia/ reperfusion group (PMA), SOD, XOD and free calcium and mitochondrial respiration in L-6TG cell were evaluated in each group. Apoptosis was detected by flow cytometer with PI staining method and agarose gel electrophoresis, the immunohistochemical method was used to determine the expression of caspase-3.
  
  
  RESULTS
  Compared with I/R group, in PMA group, XOD , free calcium in L-6TG cell and apoptotic percentage all decreased significantly, while SOD and mitochondrial respiration in L-6TG cell increased. DNA fragmentation analysis of L-6TG cell showed no laddering pattern. The expression of caspase-3 was down regulated significantly.
  
  
  CONCLUSIONS
  Activation of PKC can lessen ischemia/reperfusion injury and apoptosis through lessening oxidative injury and mitochondrial injury, adjusting calcium dyshomeostasis and down expression of caspase-3.
authors.affiliation: Department of Pathophysiology of North China Coal Medical College, Tangshan, 063000, China.
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Li
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Kong
authors.forename: Lian-Yuan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Dong
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Men
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D008928:Mitochondria / Q000378:metabolism; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000378:metabolism; D018384:Oxidative Stress; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D011493:Protein Kinase C; C505410:Casp3 protein, rat; D053148:Caspase 3; D002118:Calcium