title: Mitogenic response and cytokine production induced by cramoll 1,4 lectin in splenocytes of inoculated mice.
abstract: Cramoll 1,4 is a lectin with specific glucose/mannose binding, which is extracted from seeds of Cratylia mollis Mart. Many assays have shown the cytokine expression activity and anti-inflammatory profile of this lectin. The aim of this study was to evaluate the immunostimulatory response, in vitro, of splenocytes in mice previously inoculated, in vivo, with C. mollis (Cramoll 1,4) and Canavalia ensiformis (Con A) lectins. Results demonstrated higher proliferation indexes induced by Cramoll 1,4 than Con A lectin in relation to all experimental groups. Cramoll 1,4 and Con A also induced high levels of IL-2, IL-6, IFN-γ and nitric oxide production. Moreover, Cramoll 1,4 did not induce apoptosis and stimulated a significant number of cells in the S phase of the cell cycle. Results showed that Cramoll 1,4 lectin induces proliferative response and suggested that this lectin can be used as a mitogenic agent in immunostimulatory assays.
authors.affiliation: Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil. crismout_melo@hotmail.com
authors.forename: C M L
authors.initials: CM
authors.lastname: de Melo
authors.forename: H
authors.initials: H
authors.lastname: Melo
authors.forename: M T S
authors.initials: MT
authors.lastname: Correia
authors.forename: L C B B
authors.initials: LC
authors.lastname: Coelho
authors.forename: M B
authors.initials: MB
authors.lastname: da Silva
authors.forename: V R A
authors.initials: VR
authors.lastname: Pereira
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D008938:Mitosis; D009569:Nitric Oxide / Q000096:biosynthesis; D037121:Plant Lectins / Q000276:immunology*; D013154:Spleen / Q000166:cytology* / Q000276:immunology*
chemical_list: D007376:Interleukin-2; D015850:Interleukin-6; D037121:Plant Lectins; D009569:Nitric Oxide; D007371:Interferon-gamma