title: Up-regulation of Gr1+CD11b+ population in spleen of dextran sulfate sodium administered mice works to repair colitis.
abstract: Dextran sulfate sodium (DSS) is commonly used in rodent IBD models to chemically induce acute intestinal inflammation. The acute course of colitis includes colon tissue damages and recovery from wounded tissues. As skin wound repair was delayed by splenectomy, we asked whether splenectomy would induce the delay of colonic wound healing. In splenectomized mice, body weight recovery, disease score and colon length were delayed. Surprisingly we found a great increase of Gr1+CD11b+ cells in spleen and bone marrow of DSS-administered mice. Anti-Gr-1 antibody treatment worsened the DSS- administered colitis. These results indicate that Gr1+CD11b+ cells induced by DSS worked to repair colon wound healing and repair colitis.
authors.affiliation: Department of Immunology, Nagoya University Graduate School of Medicine, 65 Turumai-cho, Showa-ku, Nagoya, Aichi 466-8520, Japan.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Sachiko
authors.initials: S
authors.lastname: Ito
authors.forename: Naomi
authors.initials: N
authors.lastname: Nishio
authors.forename: Zhao
authors.initials: Z
authors.lastname: Cheng
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Suzuki
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Isobe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D000950:Antigens, Ly / Q000276:immunology / Q000378:metabolism*; D001853:Bone Marrow / Q000276:immunology; D039481:CD11b Antigen / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D003106:Colon / Q000276:immunology* / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D007275:Injections, Intravenous; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology*; D012720:Severity of Illness Index; D013154:Spleen / Q000276:immunology; D013156:Splenectomy; D013997:Time Factors; D015854:Up-Regulation; D014945:Wound Healing
chemical_list: D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D039481:CD11b Antigen; C480309:Ly6G antigen, mouse; D016264:Dextran Sulfate