title: Isolinderanolide B, a butanolide extracted from the stems of Cinnamomum subavenium, inhibits proliferation of T24 human bladder cancer cells by blocking cell cycle progression and inducing apoptosis.
abstract: Isolinderanolide B (IOB), a butanolide extracted from the stems of Cinnamomum subavenium, was investigated for its antiproliferative activity in T24 human bladder cancer cells. To identity the anticancer mechanism of IOB, its effect on apoptosis, cell cycle distribution, and levels of p53, p21 Waf1/Cip1, Fas/APO-1 receptor, and Fas ligand was assayed. Enzyme-linked immunosorbent assay showed that the G0/G1 phase arrest is because of increase in the expression of p21 Waf1/Cip1. An enhancement in Fas/APO-1 and membrane-bound Fas ligand (mFasL) might be responsible for the apoptotic effect induced by IOB. This study reports the novel finding that the induction of p21 Waf1/Cip1 and activity of the Fas/mFas ligand apoptotic system may participate in the antiproliferative activity of IOB in T24 cells.
authors.affiliation: Chi Mei Medical Center, Tainan, Taiwan.
authors.forename: Kun-Hung
authors.initials: KH
authors.lastname: Shen
authors.forename: En-Shyh
authors.initials: ES
authors.lastname: Lin
authors.forename: Po-Lin
authors.initials: PL
authors.lastname: Kuo
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Hsu
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053181:Caspase 8 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D027424:Cinnamomum; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D019014:fas Receptor / Q000378:metabolism
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D053222:Fas Ligand Protein; D010936:Plant Extracts; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; C571823:isolinderanolide B; D053181:Caspase 8; D015107:4-Butyrolactone