title: Preparation of polydiacetylene immobilized optically encoded beads.
abstract: Polydiacetylene (PDA), which can change the chromic and fluorescence properties by inducing environmental perturbations, is immobilized on planar solid supports for many biological applications. In this work, we immobilize PDA onto optically encoded spherical beads (PDA-SERS beads). The prepared PDA immobilized beads (36 μm) exhibit a blue color without fluorescence. By inducing stress, their color and fluorescence properties are changed to red with fluorescence. The SERS spectra of the PDA-SERS beads can be recognized over the PDA background. Moreover, our PDA immobilization methods are successfully applied to silica-surface SERS-encoded beads (5 μm) and proven to also be useful in fluorescence encoding systems.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Bong-Hyun
authors.initials: BH
authors.lastname: Jun
authors.forename: Jongho
authors.initials: J
authors.lastname: Baek
authors.forename: Homan
authors.initials: H
authors.lastname: Kang
authors.forename: Young June
authors.initials: YJ
authors.lastname: Park
authors.forename: Dae Hong
authors.initials: DH
authors.lastname: Jeong
authors.forename: Yoon-Sik
authors.initials: YS
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003116:Color; D005453:Fluorescence; D006358:Hot Temperature; D008863:Microspheres; D055620:Optical Phenomena; D010316:Particle Size; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D012822:Silicon Dioxide