title: [Effects of acute hypoxia and adenosine on splenic T-lymphocyte proliferation of rats].
abstract: OBJECTIVE
  To observe the effects of acute hypoxia and adenosine on splenic T lymphocyte proliferation.
  
  
  METHODS
  Wistar rats were divided into control and hypoxic group, and the latter were exposed to hypoxia (5000 m simulated high altitude, 23 h/d). Three days later, spleen cells were collected and stimulated by 5.0 microg/ml and 2.5 microg/ml concanavalin A (ConA) to determine the splenocyte proliferation. The proliferation was also observed after addition of different amount of adenosine to culture medium.
  
  
  RESULTS
  Acute hypoxia and adenosine had marked inhibitory effect on mitogenic response to Con A in splenic T cells, and the inhibitory effect induced by adenosine displayed concentration-dependent.
  
  
  CONCLUSIONS
  Acute hypoxia may impair the T cell function and adenosine could be involved in this process.
authors.affiliation: Department of Pathophysiology and Institute of High Altitude Medicine, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Qing-yuan
authors.initials: QY
authors.lastname: Huang
authors.forename: Guo-bin
authors.initials: GB
authors.lastname: Zhang
authors.forename: Li-fei
authors.initials: LF
authors.lastname: Cao
authors.forename: Yu-qi
authors.initials: YQ
authors.lastname: Gao
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D000860:Hypoxia; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects*
chemical_list: D003470:Culture Media; D003208:Concanavalin A; D000241:Adenosine