title: Glucose and fructose uptake by Limulus polyphemus hepatopancreatic brush border and basolateral membrane vesicles: evidence for Na+-dependent sugar transport activity.
abstract: [(3)H]-fructose and [(3)H]-glucose transport activities were determined in brush border membrane vesicles (BBMV) and basolateral membrane vesicles (BLMV) from Limulus polyphemus (horseshoe crab) hepatopancreas. Glucose transport was equilibrative in the absence of sodium and sodium dependent in the presence of sodium in BBMV, suggesting GLUT-like and SGLT-like transport activity. Glucose transport by BLMV was equilibrative and sodium independent. Fructose uptake by BBMV and BLMV was equilibrative in the absence of sodium and sodium dependent in the presence of sodium. Western blot analysis using a rabbit anti-mouse SGLT-1 polyclonal antibody indicated the presence of a cross-reacting horseshoe crab BBMV protein of similar molecular weight to the mammalian SGLT1. Sequence alignment of the mouse SGLT-4 and SGLT1 with a translated, horseshoe crab-expressed sequence tag also indicated significant identity between species. Fructose and glucose uptake in the absence and presence of sodium by hepatopancreas BBMV and BLMV indicated the presence of sodium-dependent transport activity for each sugar that may result from the presence of transporters similar to those described for other species.
authors.affiliation: Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL 32224, USA.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Sterling
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Ahearn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism*; D006737:Horseshoe Crabs / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D051379:Mice; D008871:Microvilli / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry; D016415:Sequence Alignment; D012964:Sodium / Q000502:physiology*; D051269:Sodium-Glucose Transporter 1 / Q000737:chemistry
chemical_list: D012333:RNA, Messenger; D051269:Sodium-Glucose Transporter 1; D005632:Fructose; D012964:Sodium; D005947:Glucose