title: Cytotoxicity of, and innate immune response to, size-controlled polypyrrole nanoparticles in mammalian cells.
abstract: Monodisperse polypyrrole (PPy) nanoparticles with five different diameters (20, 40, 60, 80, and 100 nm) were fabricated via chemical oxidation polymerization in order to evaluate size-dependent cytotoxicity. The cellular uptake of PPy nanoparticles in human lung fibroblasts (IMR90) and mouse alveolar macrophages (J774A.1) was observed by transmission electron microscopy. The nanoparticles were internalized into the IMR90 via endocytosis. In the J774A.1, the nanoparticles were entered via phagocytosis and endocytosis. Endocytosed nanoparticles were transported to lysosome via endosome-network. The cytotoxicity and innate immune response of PPy-treated cells were systematically investigated by viability assay, oxidative stress, apoptosis/necrosis, and expression of costimulatory molecules. The viability, oxidative stress, and apoptosis/necrosis of PPy-treated cells revealed size- and dose-dependency. Because of phagocytosis, PPy treatment had more adverse effects on the J774A.1 than the IMR90. Innate immune response of PPy-treated macrophages was measured by the expression of costimulatory molecules on surface of the cells. The expression of costimulatory molecules involved in Th1 response (CD40 and CD80) was lightly up-regulated and the other costimulatory molecule related in Th2 response (CD86) was less expressed than a negative control. These findings may provide better nanotoxicological information of polymer nanomaterials, and support the further development of PPy nanoparticles in bioelectronic applications.
authors.affiliation: World Class University program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, 599 Gwanangro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Sojin
authors.initials: S
authors.lastname: Kim
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Oh
authors.forename: Yoon Seon
authors.initials: YS
authors.lastname: Jeong
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Hong
authors.forename: Bo-Ram
authors.initials: BR
authors.lastname: Cho
authors.forename: Ji-Sook
authors.initials: JS
authors.lastname: Hahn
authors.forename: Jyongsik
authors.initials: J
authors.lastname: Jang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009336:Necrosis; D010316:Particle Size; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D011758:Pyrroles / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011108:Polymers; D011758:Pyrroles; D017382:Reactive Oxygen Species; C067635:polypyrrole