title: Fluorescent labeling of human albumin using the new aromatic dialdehyde labels and the study of innerfilter effect.
abstract: The labels naphthalene-2,3-dicarboxaldehyde (NDA), 1-phenylnaphthalene-2,3-dialdehyde (ΦNDA), and anthracene-2,3-dialdehyde (ADA) have been used as fluorigenic reagents. They formed fluorescent derivatives with proteins. The derivatives formed are in fact isoindoles. The fluorescence decay of the labels-antibody was found to extend over a period of 4, 8, and 10 h for ΦNDA, ADA, and NDA-derivative, respectively. Protein formed is comparatively less stable as compared to simple amino acids. In relation to innerfilter effect, the addition of cytochrome C, myoglobin, and ATP as absorbers to label-human albumin fluorophores appeared to have quenched the fluorescence. In the case of using NDA as label, the fluorescence was quenched roughly 70%, 24%, and 58% for addition of cytochrome C, myoglobin, and ATP, respectively. The labels used were found to give rapid, reproducible, and reliable results.
authors.affiliation: Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi - 746 00, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Aminuddin
authors.forename: Sofia
authors.initials: S
authors.lastname: Ahmed
authors.forename: Muhammd Ali
authors.initials: MA
authors.lastname: Sheraz
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahmad
authors.forename: Karamat
authors.initials: K
authors.lastname: Mahmood
authors.forename: J N
authors.initials: JN
authors.lastname: Miller