title: [Expression and function analysis of dengue virus type 1 to 4 envelope domain III recombinant fusion protein].
abstract: OBJECTIVE
  To observe the ability of dengue virus type 1-4 envelope domain III fusion protein to inhibit virus infection and analyze the neutralizing ability of polyclonal antibodies against rE III.
  
  
  METHODS
  After being connected by linker peptide, E III protein of Dengue virus serotypes 1-4 were expressed in E coli BL21 (DE3) then purified. Fusion proteins were verified by Western Blot and ELISA. Rabbits were immunized with fusion proteins to produce anti-rE III serum. The activity of anti-rE III serum were detected through indirect immunofluorescence assay test. Inhibition of dengue virus type 1 to 4 infection in BHK-21 cells by rE III fusion protein were tested. Neutralizing activity of anti-rE III serum was analyzed.
  
  
  RESULTS
  Dengue virus type 1 to 4 envelope domain III recombinant fusion protein was expressed in E coli BL21 and purified successfully. Then rE III fusion protein and anti-rE III serum were analyzed respectively and rE III fusion protein can effectively inhibit dengue virus type 1 to 4 from infecting BHK cells. The anti-rE III serums can neutralize dengue virus type 1 to 4 but with different neutralizing titer.
  
  
  CONCLUSIONS
  Dengue virus type 1-4 envelope domain III fusion protein can directly inhibit DV infection. Antibodies induced by rE III fusion proteins can neutralize dengue virus type 1-4.
authors.affiliation: Medical University, Ningxia 750003, China.
authors.forename: Guo-Yu
authors.initials: GY
authors.lastname: Niu
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.forename: Quan-Fu
authors.initials: QF
authors.lastname: Zhang
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Liang
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003716:Dengue Virus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D050939:Gene Fusion / Q000235:genetics*; D015686:Gene Products, env / Q000235:genetics* / Q000378:metabolism; D007114:Immunization; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology
chemical_list: D015686:Gene Products, env; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins