title: [The effects of protooncogene on oocyte maturation mediated by cytokines].
abstract: OBJECTIVE
  The mechanisms of cytokines in regulating oocyte maturation is still little known. The present study attempt to investigate whether the protooncogene of c-erbB2, c-myb are involved in introducing of cytokines to regulate oocyte maturation.
  
  
  METHODS
  This research used mouse GV stage oocyte culture model in vitro and RT-PCR, Western blotting method to explore the effect of EGF, TNFalpha, ET-1 and NO on oocyte maturation; to analyze the c-erbB2 mRNA and c-myb mRNA expression and the phosphorylation of MAPK and cyclinB1 expression in oocytes affected by above cytokines.
  
  
  RESULTS
  EGF(10 microg/L) stimulated meiosis of oocytes significantly, the level of c-erbB2 mRNA, c-myb mRNA were increased, and promoted the phosphorylation of MAPK and cyclinB1 expression; TNFalpha (1 microg/L) and ET-1 ((10(-1) mol/L) had the results to EGF. Low dose of SNP (10(-5)mol/L) had no effect on oocyte maturation, but could significantly reverse the suppression of dbcAMP on oocyte maturation.
  
  
  CONCLUSIONS
  c-erbB2 and c-myb were involved in introducing of cytokines to regulate oocyte maturation, might be the middle link in connection of the cytokines with MAPK and MPF in regulation oocyte maturation.
authors.affiliation: Department of Physiology, Nanchang University, Nanchang 330006, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zheng
authors.forename: Jing-Lei
authors.initials: JL
authors.lastname: Wang
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Qiu-Xiang
authors.initials: QX
authors.lastname: Xiao
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000502:physiology; D005260:Female; D000077155:Granulins; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology; D016200:Maturation-Promoting Factor / Q000235:genetics / Q000378:metabolism; D000090204:Mesothelin; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects / Q000502:physiology*; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D016207:Cytokines; D004815:Epidermal Growth Factor; D000077155:Granulins; D036341:Intercellular Signaling Peptides and Proteins; D016200:Maturation-Promoting Factor; D000090204:Mesothelin; D020928:Mitogen-Activated Protein Kinases; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D014409:Tumor Necrosis Factor-alpha; C000715528:Msln protein, mouse