title: Characterization of microsatellite loci in the SLA class I region.
abstract: Microsatellite (MS) markers in the SLA-1 region were characterized via sequencing analysis with BAC clones generated from the National Institute of Health miniature pigs (MIPs). A total of 16 BAC clones were sequenced producing 15,228 shotgun reads, corresponding to 11.2 X sequencing coverage, that were used to construct a contig of 12.18 Mb in length. MS markers were compared with previously deposited GenBank sequences to verify the existence of 423 potential MS candidate markers in the SLA-1 region. Evaluation of these polymorphisms confirmed 59 markers in MIPs, and the combined data including sequences from GenBank revealed 155 polymorphic MS markers. MS markers identified from this analysis can be used to provide an alternative method to direct typing for determining an individual's SLA-1 haplotype.
authors.affiliation: Animal Genetic Improvement Division, National Institute of Animal Science, Seonghwan, Cheonan, 333801, Republic of Korea. chung133@korea.kr
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Chung
authors.forename: Matthew Charles
authors.initials: MC
authors.lastname: McClure
mesh_terms: D000818:Animals; D001483:Base Sequence; D014644:Genetic Variation; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics; D013552:Swine / Q000235:genetics; D013556:Swine, Miniature / Q000235:genetics*
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen