title: NAD+ treatment decreases tumor cell survival by inducing oxidative stress.
abstract: NAD+ plays important roles in various biological processes. It has been shown that NAD+ treatment can decrease genotoxic agent-induced death of primary neuronal and astrocyte cultures, and NAD+ administration can reduce ischemic brain damage. However, the effects of NAD+ treatment on tumor cell survival are unknown. In this study we found that treatment of NAD+ at concentrations from 10 micromolar to 1 mM can significantly decrease the survival of various types of tumor cells such as C6 glioma cells. In contrast, NAD+ treatment did not impair the survival of primary astrocyte cultures. Our study has also indicated that oxidative stress mediates the effects of NAD+ on the survival of tumor cells, and P2X7 receptors and altered calcium homeostasis are involved in the effects of NAD+ on the cell survival. Collectively, our study has provided the first evidence that NAD+ treatment can decrease the survival of tumor cells by such mechanisms as inducing oxidative stress. Because NAD+ treatment can selectively decrease the survival of tumor cells, NAD+ may become a novel agent for treating cancer.
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Zhao
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Hong
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Ma
authors.forename: Heyu
authors.initials: H
authors.lastname: Chen
authors.forename: Weiliang
authors.initials: W
authors.lastname: Xia
authors.forename: Weihai
authors.initials: W
authors.lastname: Ying
mesh_terms: D000704:Analysis of Variance; D001253:Astrocytes / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004996:Ethidium / Q000031:analogs & derivatives; D005434:Flow Cytometry; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009243:NAD / Q000378:metabolism / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism; D014343:Trypan Blue
chemical_list: D058486:Receptors, Purinergic P2X7; D009243:NAD; C067883:dihydroethidium; D007770:L-Lactate Dehydrogenase; D004996:Ethidium; D014343:Trypan Blue; D002118:Calcium