title: Spectrophotometric and spectrofluorimetric methods for determination of Racecadotril.
abstract: Two accurate and sensitive spectrophotometric and spectrofluorimetric methods were developed for determination of Racecadotril. In the first method reduction of Fe3+ into Fe2+ in presence of o-phenanthroline by Racecadotril to form a stable orange-red ferroin chelate [Fe-(Phen)3]2+ was the basis for its determination. The absorbance at 510 nm was measured and linear correlation was obtained in the concentration range of 2.5-25 µg mL(-1). In the second method the native fluorescence of Racecadotril in acetonitrile solvent at λ=319 nm when excitation was at 252 nm is used for its determination. Linear correlation was obtained in the concentration range of 50 to 500 ng mL(-1). The proposed methods were applied for determination of Racecadotril in bulk powder with mean accuracy of 100.39±1.239 for the spectrophotometric method and 100.09±1.042 for the spectrofluorimetric method. The proposed methods were successfully applied for determination of Racecadotril in its pharmaceutical dosage form.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Beni-Sueif University, Beni-Sueif Egypt. dr.nourali@hotmail.com
authors.forename: Nouruddin Wageih
authors.initials: NW
authors.lastname: Ali
authors.forename: Mohammed Refaat
authors.initials: MR
authors.lastname: Elghobashy
authors.forename: Mohammed Gamal
authors.initials: MG
authors.lastname: Mahmoud
authors.forename: Mohammed Abdelkawy
authors.initials: MA
authors.lastname: Mohammed
mesh_terms: D000930:Antidiarrheals / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D007502:Iron Chelating Agents / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D011208:Powders; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D015244:Thiorphan / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000930:Antidiarrheals; D007502:Iron Chelating Agents; D010618:Phenanthrolines; D011208:Powders; D012996:Solutions; C049331:racecadotril; D015244:Thiorphan; D007501:Iron; C025205:1,10-phenanthroline