title: [Determination of rat serum emodin level by microemulsion liquid chromatography with direct sample loading].
abstract: OBJECTIVE
  To establish a microemulsion liquid chromatography system with direct sample loading for determining the serum level of emodin in rats.
  
  
  METHODS
  The separation was performed on C₁₈ column (Hypersil BDS, 5 µm,150 mm×4.6 mm) with the microemulsion mobile phase consisting of 3.3% (w/V) SDS, 6.6% (V/V) n-butyl alcohol, and 1.0% (V/V) octane and water. The flow rate was 1.0 ml/min and the detection wavelength was 254 nm.
  
  
  RESULTS
  The linear range of emodin detection was 0.333-5.32 µg/ml. The average recovery was 99.65% with a RSD of 3.60%. The limit of quantification was 0.1386 µg/mL.
  
  
  CONCLUSIONS
  Microemulsion liquid chromatography system with direct sample loading allows simple, accurate and rapid determination of emodin in rat serum.
authors.affiliation: Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China. shiyong163sy@sina.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Shi
authors.forename: Bing-jun
authors.initials: BJ
authors.lastname: Wang
authors.forename: Xiao-kai
authors.initials: XK
authors.lastname: Lin
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Huang
authors.forename: Kai-qian
authors.initials: KQ
authors.lastname: Lin
authors.forename: Shou-yao
authors.initials: SY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004642:Emodin / Q000097:blood*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000737:chemistry*
chemical_list: D004642:Emodin