title: Establishment of the cell line expressing human prion protein on PrP0/0 background.
abstract: In this work we described preparation of the novel cell lines expressing human prion protein on PrP0/0-background. Prepared cell lines originated from the Nagasaki mice (PrP0/0) and showed a fibroblast phenotype. The expression level of human prion protein in the developed cell lines was comparable to the physiological expression measured in GT1-7 cells. A great advantage of the prepared cell lines was their short doubling time that allowed obtaining of a large amount of cells for the proteomic experiments. Newly established cell lines open a broad spectrum of applications in the prion research. Besides the study of physiological function of the prion protein or its interactome, the new cell lines could be successfully employed as a unique tool for the better understanding of key events in the pathogenesis of prion diseases.
authors.affiliation: Slovak Academy of Sciences, Bratislava, Slovak Republic. michal.prcina@savba.sk
authors.forename: M
authors.initials: M
authors.lastname: Prčina
authors.forename: P
authors.initials: P
authors.lastname: Filipčík
authors.forename: E
authors.initials: E
authors.lastname: Kontseková
mesh_terms: D000818:Animals; D002460:Cell Line / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D041681:NIH 3T3 Cells; D018621:PrPC Proteins / Q000235:genetics* / Q000378:metabolism; D017096:Prion Diseases / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D018621:PrPC Proteins