title: Automatic system for analysis of locomotor activity in rodents--a reproducibility study.
abstract: Automatic analysis of locomotion in studies of behavior and development is of great importance because it eliminates the subjective influence of evaluators on the study. This study aimed to develop and test the reproducibility of a system for automated analysis of locomotor activity in rats. For this study, 15 male Wistar were evaluated at P8, P14, P17, P21, P30 and P60. A monitoring system was developed that consisted of an open field of 1m in diameter with a black surface, an infrared digital camera and a video capture card. The animals were filmed for 2 min as they moved freely in the field. The images were sent to a computer connected to the camera. Afterwards, the videos were analyzed using software developed using MATLAB® (mathematical software). The software was able to recognize the pixels constituting the image and extract the following parameters: distance traveled, average speed, average potency, time immobile, number of stops, time spent in different areas of the field and time immobile/number of stops. All data were exported for further analysis. The system was able to effectively extract the desired parameters. Thus, it was possible to observe developmental changes in the patterns of movement of the animals. We also discuss similarities and differences between this system and previously described systems.
authors.affiliation: Department of Nutrition, University Federal of Pernambuco, Recife, PE, Brazil.
authors.forename: Raquel da Silva
authors.initials: Rda S
authors.lastname: Aragão
authors.forename: Marco Aurélio Benedetti
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: Karla Mônica Ferraz Teixeira
authors.initials: KM
authors.lastname: de Barros
authors.forename: Sebastião Rogério Freitas
authors.initials: SR
authors.lastname: Silva
authors.forename: Ana Elisa
authors.initials: AE
authors.lastname: Toscano
authors.forename: Ricardo Emmanuel
authors.initials: RE
authors.lastname: de Souza
authors.forename: Raul
authors.initials: R
authors.lastname: Manhães-de-Castro
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001835:Body Weight; D001330:Electronic Data Processing / Q000379:methods*; D008297:Male; D008991:Monitoring, Physiologic; D009043:Motor Activity / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012984:Software; D013997:Time Factors