title: A model for analysis of the yield and the level of clustering of radiation-induced DNA-strand breaks in hydrated plasmids.
abstract: Although it is widely accepted that the spatial distribution of strand breaks is highly relevant to the biological consequences of radiation, the extent to which strand breaks are clustered is not usually demonstrated directly from the experimental data. To evaluate the spatial distribution of radiation-induced strand breaks, the authors have developed a model for the generation of strand breaks after irradiation. The model assumes that (1) a radiation track has a certain probability of 'hitting' a plasmid, (2) the radiation generates strand break(s) by chance within a relatively small region that could produce double-strand breaks and (3) the number of strand breaks generated within the region follows a Poisson distribution. To find out whether the model is valid, the authors compared the calculated values with the experimental data obtained by a plasmid DNA assay. Taking into account the inherent bias of the plasmid assay, the model described well the experimental results of hydrated plasmids exposed to radiation.
authors.affiliation: Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai-mura, Ibaraki 319-1195, Japan. shikazono.naoya@jaea.go.jp
authors.forename: N
authors.initials: N
authors.lastname: Shikazono
authors.forename: A
authors.initials: A
authors.lastname: Yokoya
authors.forename: A
authors.initials: A
authors.lastname: Urushibara
authors.forename: M
authors.initials: M
authors.lastname: Noguchi
authors.forename: K
authors.initials: K
authors.lastname: Fujii
mesh_terms: D000818:Animals; D003198:Computer Simulation; D004249:DNA Damage / Q000502:physiology*; D006801:Humans; D018499:Linear Energy Transfer / Q000502:physiology*; D008956:Models, Chemical; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics* / Q000528:radiation effects*; D011829:Radiation Dosage; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water