title: In vitro generation of immune cells from pluripotent stem cells.
abstract: Stem cell transplant recipients and acquired or inherited immune-deficiency patients could benefit from the infusion of B, T and/or NK cells. These lymphoid cells can be generated in vitro from bone marrow derived CD34+CD45+ hematopoietic stem cells (HSC). The number of cells that can be obtained in this way is limited especially in the adult. An alternative source may therefore constitute human pluripotent stem cells (PSC) such as embryonic (hESC) or induced pluripotent stem cells (hiPSC). Here, we focus on present knowledge on the generation of lymphoid cells from hESC. The two main obstacles for the generation of clinically relevant immune cells are the failure to generate from hESC long-term repopulating HSC which could be kept in culture for prolonged time; and insufficient knowledge of the selection process which generates mature T cells from CD4 CD8 double positive (DP) precursors in vitro.
authors.affiliation: Department of Clinical Chemistry, University of Ghent, Ghent, Belgium. bart.vandekerckhove@ugent.be
authors.forename: Bart
authors.initials: B
authors.lastname: Vandekerckhove
authors.forename: Stijn
authors.initials: S
authors.lastname: Vanhee
authors.forename: Stefanie
authors.initials: S
authors.lastname: Van Coppernolle
authors.forename: Sylvia
authors.initials: S
authors.lastname: Snauwaert
authors.forename: Imke
authors.initials: I
authors.lastname: Velghe
authors.forename: Tom
authors.initials: T
authors.lastname: Taghon
authors.forename: Georges
authors.initials: G
authors.lastname: Leclercq
authors.forename: Tessa
authors.initials: T
authors.lastname: Kerre
authors.forename: Jean
authors.initials: J
authors.lastname: Plum
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D001402:B-Lymphocytes / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D007694:Killer Cells, Natural / Q000166:cytology; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology*; D033581:Stem Cell Transplantation; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D018952:Antigens, CD34