title: Clinical presentation and molecular characterization of group B rotaviruses in diarrhoea patients in Bangladesh.
abstract: A total of 1106 stool samples collected from diarrhoea patients admitted to Dhaka hospital of the International Centre for Diarrhoeal Disease Research, Bangladesh, during January-December 2008 were analysed for the presence of rotavirus-specific RNA by PAGE. The group B-specific RNA migration pattern was detected in 26 patients (2.4%) and group A-specific pattern in 259 patients (23.4%). Clinical data from group A and group B rotavirus-infected patients indicated that episodes did not differ much in the prevalence of diarrhoea, number of stools, outcome or differences in gender. However, abdominal pain was more common in group B rotavirus infections (36 vs 15%, P=0.02) and the virus was responsible for more severe dehydration compared with group A-infected patients (12 vs 3%, P=0.04). Sequence analyses of VP4, VP7 and NSP2 indicated that an Indian-Bangladeshi lineage of the virus, which is different from both the prototype (Chinese) lineage and from the animal group B rotaviruses, has been circulating in Bangladesh. Continuous monitoring of group B rotaviruses both in hospitals and in the community will be helpful to determine the true burden of group B rotaviruses.
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Farjana
authors.initials: F
authors.lastname: Saiada
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: H N Ashiqur
authors.initials: HNA
authors.lastname: Rahman
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Sayra
authors.initials: S
authors.lastname: Moni
authors.affiliation: Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh.
authors.forename: M Manjurul
authors.initials: MM
authors.lastname: Karim
authors.affiliation: Laboratory of Clinical and Epidemiological Virology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.
authors.forename: Mahmoud Reza
authors.initials: MR
authors.lastname: Pourkarim
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Tasnim
authors.initials: T
authors.lastname: Azim
authors.affiliation: Virology Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Mohakhali, Dhaka 1212, Bangladesh.
authors.forename: Mustafizur
authors.initials: M
authors.lastname: Rahman
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001459:Bangladesh; D002648:Child; D016000:Cluster Analysis; D003967:Diarrhea / Q000473:pathology* / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000473:pathology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012367:RNA, Viral