title: [Effect of hypoxia on the expressions of intermedin/ adrenomedullin2 in plasma and the tissues of heart and lung in rats].
abstract: OBJECTIVE
  To study the effect and significances of two-week hypoxia on the expression of intermedin/adrenomedullin2 (IMD/ADM2) in plasma and the tissues of heart and lung in rats.
  
  
  METHODS
  Twenty male SD rats were randomly divided into normal control group and hypoxia group. The concentrations of IMD/ADM2 and adrenomedullin (ADM) in plasma, right ventricle and lung tissue were measured by radioimmunoassay. RT-PCR was used to detect the mRNA levels of IMD/ADM2 and ADM in right ventricle and lung tissue.
  
  
  RESULTS
  (1) The mean pulmonary arterial pressure (mPAP) and the weight ratio of right ventricle (RV) to left ventricle plus septum (LV + S) of hypoxia group were significantly higher than those of normal control group (P < 0.01). (2) The concentrations of IMD/ADM2 and ADM in plasma were significantly higher in hypoxia group, compared with normal control group (P < 0.01). (3) The concentration of ADM in right ventricle and lung tissue in hypoxia group was significantly higher than that in normal control group (P < 0.01), while there was no significant difference in IMD/ADM2 between the two groups. (4) The mRNA levels of IMD/ADM2 and ADM in right ventricle and lung tissues were significantly up-regulated in hypoxia group (P < 0.05).
  
  
  CONCLUSIONS
  The expressions of IMD/ADM2 peptides and gene in plasma, right ventricular and pulmonary tissues are different in the early-middle pathological proceeding of pulmonary hypertension induced by two-week hypoxia in rats.
authors.affiliation: Institute of Cor Pulmonale, Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Gong
authors.forename: Lii
authors.initials: L
authors.lastname: Zhang
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Guo
authors.forename: Liang-Gang
authors.initials: LG
authors.lastname: Hu
authors.forename: Sun-Zhong
authors.initials: SZ
authors.lastname: Mao
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Fan
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Hong
mesh_terms: D053607:Adrenomedullin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000378:metabolism*; D000860:Hypoxia / Q000150:complications / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009479:Neuropeptides / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C499411:Adm2 protein, rat; D009479:Neuropeptides; D012333:RNA, Messenger; D053607:Adrenomedullin