title: A reagentless, disposable and multiplexed electronic biosensing platform: application to molecular logic gates.
abstract: The construction of a reagentless, sensitive, disposable and multiplexed electronic sensing platform for one-spot simultaneous determination of biomolecules with significant difference in size (proteins and small molecules) is described. The sensing surface is fabricated by the hybridization of two types of redox-tags conjugated aptamers with the corresponding complementary DNAs, which are self-assembled on a gold nanoparticle-modified screen printed carbon electrode. The presence of the target analytes leads to the release of the tagged signaling aptamers from the sensing surface, and the surface-remained tags exhibit well-resolved peaks, whose positions and sizes reflect the identities and concentrations of the target analytes, respectively. The application of the proposed sensing platform for molecular logic gate operations is also demonstrated.
authors.affiliation: Key Laboratory of Ministry of Education on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. yunatswu@swu.edu.cn
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Qian
authors.forename: Yuyong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D002244:Carbon; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold; D008128:Logic; D053768:Metal Nanoparticles; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D011506:Proteins; D002244:Carbon; D006046:Gold; D000255:Adenosine Triphosphate; D013917:Thrombin