title: Proteomic analysis of egg white proteins during storage.
abstract: Egg storage causes egg white to lose its viscous nature to form a thin liquid, commonly referred to as egg white thinning. To understand the mechanisms underlying egg white thinning, white-shell eggs were used in the present study to determine the proteome-level changes of egg white proteins occurred during storage. Egg white thinning was observed visually after 20 days of storage at ambient temperature (22 ± 2 °C) when the maximum number of proteome-level changes occurred. The proteins that showed significant changes in abundance during storage included ovalbumin, clusterin, ovoinhibitor, ovotransferrin, and prostaglandin D2 synthase. Among these, only the abundance of clusterin was observed to change continuously during the storage period. Hence, it is expected that the increase in the concentrations of clusterin and ovoinhibitor along with the change of ovalbumin content during storage might contribute to egg white thinning. Degradation of ovalbumin/clusterin during egg storage may be due to the combined effect of proteolysis and increase in pH; this may also be partly responsible for egg white thinning phenomenon.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Dileep A
authors.initials: DA
authors.lastname: Omana
authors.forename: Yue
authors.initials: Y
authors.lastname: Liang
authors.forename: Nat N V
authors.initials: NN
authors.lastname: Kav
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D002645:Chickens; D051152:Clusterin / Q000378:metabolism; D003207:Conalbumin / Q000378:metabolism; D004527:Egg Proteins / Q000378:metabolism*; D015387:Egg Proteins, Dietary / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005519:Food Preservation; D010047:Ovalbumin / Q000378:metabolism; D013696:Temperature
chemical_list: D051152:Clusterin; D004527:Egg Proteins; D015387:Egg Proteins, Dietary; D003207:Conalbumin; C004224:ovoinhibitor; D010047:Ovalbumin