title: The G-protein β3 polymorphism is associated with diffuse type gastric cancer in Japanese.
abstract: From epidemiological evidence a high salt diet is a risk factor for gastric cancer, independent of Helicobacter pylori infection, and animal studies have shown that salt promotes carcinogenesis. The G-protein β 3(GNB3) C825T polymorphism has been linked with hypertension, salt sensitivity and multiple diseases. Our aim in this study was to clarify any association of the GNB3 C825T polymorphism with gastric cancer risk in Japanese. We examined 161 patients with gastric cancer and 183 control subjects. All underwent stomach biopsy by endoscopic procedures, and extracted DNA was genotyped using a primer pair including the GNB3 C825T polymorphism area by PCR-based restriction fragment length polymorphism (PCR-RFLP). Logistic-regression analysis was performed to assess the impact of the genetic polymorphism. Overall comparison of genotype frequency showed the CT genotype in control patients to be relatively infrequent, but no statistically significant differences were found. However, on comparison of subtypes of gastric cancer (intestinal and diffuse), a significantly increased risk of diffuse type of gastric cancer was foudn for the TT genotype (odds ratio compared to CC, 3.1, 95%CI 1.1-8.6, p=0.03). In conclusion, the TT genotype of GNB3 was associated with diffuse type of gastric cancer. The mechanism of the GNB3 polymorphism interaction with gastric cancer development needs to be clarified by future study.
authors.affiliation: Department of Gastroenterology, Fujita Health University School of Medicine, Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, Japan. shibat03@fujita-hu.ac.jp
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Tahara
authors.forename: Joh
authors.initials: J
authors.lastname: Yonemura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Okubo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yoshioka
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirata
mesh_terms: D000368:Aged; D005260:Female; D020022:Genetic Predisposition to Disease; D016480:Helicobacter pylori / Q000302:isolation & purification; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017673:Sodium Chloride, Dietary; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C454420:G-protein beta3 subunit; D017673:Sodium Chloride, Dietary; D020962:Heterotrimeric GTP-Binding Proteins