title: [Influence of recombined human growth hormone on sIgA and EGF in rats with obstructive jaundice].
abstract: OBJECTIVE
  To study the effect of the recombined human growth hormone(rhGH) on secretory immunoglobulin A (sIgA), epidermal growth factor (EGF) in rats with obstructive jaundice.
  
  
  METHODS
  Sixty Wistar rats were randomly divided into four groups, sham-operated (group A), common biliary duct-ligated (group B), biliary duct-ligated plus rhGH-treat for one week (group C), biliary duct-ligated plus rhGH-treat for two weeks (group D), each group had 15 rats. Except group A, the rats of other groups were operated with biliary duct-ligated. Until two weeks after operation, the rats of group A and B were killed. After operation, the rats of group C were treated with rhGH hypodermic injection (0.75 U x kg(-1) x d(-1)) for one week, and then killed. The rats of D group were treated with rhGH hypodermic injection (0.75 U x kg(-1) x d(-1)) for two weeks, and then killed. All procedures were performed aseptically. Total bilirubin (TB), alkaline phosphatase (ALP), prealbumin(PA), insulin-like growth factor (IGF-1), sIgA, EGF were measured.
  
  
  RESULTS
  Compared with group A, in group B, C, D, serum level of PA, IGF-1 and sIgA, EGF level of gastric and intestinal juice were lower, but TB, ALP were higher, there were significant difference. Compared with group B, the rats with treatment of rhGH in group C and D had higher sIgA and EGF and lower intestinal bacterial translocation.
  
  
  CONCLUSIONS
  In objective jaundice rats, rhGH can protect their hepatic function, intestinal physical-barrier function and immune-barrier function, and reduce intestinal bacterial translocation.
authors.affiliation: 1st Affiliated Hospital, University of Henan Science and Technology, Henan 471003, China.
authors.forename: Zong-li
authors.initials: ZL
authors.lastname: Sun
authors.forename: Xue-ting
authors.initials: XT
authors.lastname: Wang
authors.forename: Wu-wei
authors.initials: WW
authors.lastname: Li
authors.forename: Li-qun
authors.initials: LQ
authors.lastname: Ren
authors.forename: Lei
authors.initials: L
authors.lastname: Gu
authors.forename: Xian-li
authors.initials: XL
authors.lastname: Liu
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D004815:Epidermal Growth Factor / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D041781:Jaundice, Obstructive / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007071:Immunoglobulin A, Secretory; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D013006:Growth Hormone