title: Role of 2-oxo and 2-thioxo modifications on the fragmentation reactions of the histidine radical cation.
abstract: The fragmentation reactions of the radical cations, M(·+), of histidine, 2-oxo-histidine and 2-thioxo-histidine were examined using a combination of experiments performed on a linear ion trap and density functional theory (DFT) calculations at the UB3-LYP/6-311++G(d,p) level of theory. Low-energy collision-induced dissociation (CID) on [Cu(II)(terpy)(M)](2+) complexes, formed via electrospray ionisation, produced the radical cations in sufficient yield to examine their unimolecular chemistry via an additional stage of CID. The CID spectrum of the radical cation of histidine is dominated by loss of water with the next most abundant ion arising from the combined loss of H(2)O and CO. In contrast, the CID spectra of the radical cations of 2-oxo-histidine and 2-thioxo-histidine are dominated by the combined loss of CO(2) and NH=CH(2). The observed differences are rationalised via DFT calculations which reveal that the barrier associated with loss of CO(2) from the histidine radical cation is higher than that for loss of H(2)O. In contrast, the introduction of an oxygen or sulfur atom into the side chain of histidine results in a reversal of the order of these barrier heights, thus making CO(2) loss the preferred pathway.
authors.affiliation: School of Chemistry, The University of Melbourne, Victoria 3010, Australia.
authors.forename: Adrian K Y
authors.initials: AK
authors.lastname: Lam
authors.forename: Craig A
authors.initials: CA
authors.lastname: Hutton
authors.forename: Richard A J
authors.initials: RA
authors.lastname: O'Hair
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013455:Sulfur / Q000737:chemistry; D013457:Sulfur Compounds / Q000737:chemistry*
chemical_list: D002412:Cations; D005609:Free Radicals; D013457:Sulfur Compounds; D002245:Carbon Dioxide; C083725:2-oxohistidine; D006639:Histidine; D013455:Sulfur; D010100:Oxygen