title: High-mobility group B1 (HMGB1) and receptor for advanced glycation end-products (RAGE) expression in canine lymphoma.
abstract: BACKGROUND
  Canine lymphoma is a commonly occurring, spontaneously developing neoplasia similar to human non-Hodgkin's lymphoma and, thus, is used as a valuable model for human malignancy. HMGB1 and RAGE are strongly associated with tumour progression and vascularisation. Consequently, deregulated RAGE and HMGB1 may play an important role in the mechanisms involved in lymphoma progression.
  
  
  METHODS
  Expression patterns of HMGB1 and RAGE were analysed in 22 canine lymphoma and three canine non-neoplastic control samples via real time PCR and canine beta-glucuronidase gene (GUSB) as endogenous control.
  
  
  RESULTS
  HMGB1 was up-regulated in the neoplastic samples, while RAGE expression remained inconspicuous.
  
  
  CONCLUSIONS
  This study demonstrated similar mechanisms in lymphoma progression in humans and dogs due to overexpression of HMGB1, which was described in human lymphomas. RAGE remained stable in terms of expression indicating that the extracellular HMGB1-induced effects are regulated by HMGB1 itself.
authors.affiliation: Small Animal Clinic and the German Research Foundation SFB Transregio 37, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
authors.forename: Katharina A
authors.initials: KA
authors.lastname: Sterenczak
authors.forename: Alexa E
authors.initials: AE
authors.lastname: Joetzke
authors.forename: Saskia
authors.initials: S
authors.lastname: Willenbrock
authors.forename: Nina
authors.initials: N
authors.lastname: Eberle
authors.forename: Sandra
authors.initials: S
authors.lastname: Lange
authors.forename: Christian
authors.initials: C
authors.lastname: Junghanss
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
authors.forename: Jörn
authors.initials: J
authors.lastname: Bullerdiek
authors.forename: Daniela
authors.initials: D
authors.lastname: Simon
authors.forename: Hugo
authors.initials: H
authors.lastname: Murua Escobar
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D024243:HMGB1 Protein / Q000096:biosynthesis* / Q000235:genetics; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D024243:HMGB1 Protein; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic