title: [Effect of endomorphin-1 on hematopoietic regulatory molecules from normal human bone marrow stromal cells].
abstract: The aim of study was to investigate the regulatory effect of endomorphin-1 (EM-1) on ICAM-1 and VCAM-1 on normal human bone marrow stromal cell surface as well as IL-6 and TNF-α excreted by human normal bone marrow stromal cells. The expression of ICAM-1 and VCAM-1 was determined by flow cytometry, and the concentration of IL-6 and TNF-α was detected by ELISA. The results indicated that the expression of ICAM-1 and VCAM-1 was significantly different from the control group after being cultured with endomorphin-1 for 24 hours (p < 0.05), and different concentrations of endomorphin-1 had no effect on expressions of ICAM-1 and VCAM-1 both; there was no difference in secretion of IL-6 and TNF-α between experimental and control groups (p > 0.05). It is concluded that endomorphin-1 regulates the ICAM-1 and VCAM-1 expression on human normal bone marrow stromal cell surface, but endomorphin-1 does not display any effect on IL-6 and TNF-α secreted by human normal bone marrow stromal cells.
authors.affiliation: Lanzhou University First Hospital, Lanzhou 730000, Gansu Province, China. zhaoli@lzu.edu.cn
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Lan-Xia
authors.initials: LX
authors.lastname: Zhou
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D006413:Hematopoietic System / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D009842:Oligopeptides; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; C105293:endomorphin 1; D018799:Intercellular Adhesion Molecule-1