title: Eccentric stimulation reveals an involvement of FGF6 in muscle resistance to mechanical stress.
abstract: The objective of this report was to analyse a potential role for FGF6 in muscle resistance to mechanical stress. Normal or regenerating muscles of FGF6 (-/-) mice versus wild-type mice were submitted to different protocols of damaging eccentric contractions (eccentric electrostimulation and intermittent downhill exercise). Then muscular structural properties were analysed by histological and immunochemistry techniques to evaluate the post-injury muscle recovery; their muscle contractile parameters (maximal tetanic force, kinetics properties and fatigue resistance) were assessed. The absence of FGF6 causes (1) a fast-to-slow myofibre type switch in adult control and regenerating Tibialis anterior (TA) muscle; (2) muscle weakness in regenerating muscles in animals submitted to eccentric exercise protocols due to aberrant extensive necrotic zones. These observations point out a crucial and unexpected role for FGF6 in muscle integrity and muscle protection against mechanical stress.
authors.affiliation: UMR 8194 CNRS, Centre d'Etude de Sensori-Motricité, Centre Universitaire des Saints-Pères, Université Paris Descartes, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France.
authors.forename: Iman
authors.initials: I
authors.lastname: Laziz
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ferry
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Armand
authors.forename: Claude Louis
authors.initials: CL
authors.lastname: Gallien
authors.forename: Bruno Della
authors.initials: BD
authors.lastname: Gaspera
authors.forename: F
authors.initials: F
authors.lastname: Charbonnier
authors.forename: C
authors.initials: C
authors.lastname: Chanoine
mesh_terms: D000818:Animals; D051522:Fibroblast Growth Factor 6 / Q000235:genetics / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000235:genetics* / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000502:physiology; D053580:Muscle Strength / Q000235:genetics* / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D009135:Muscular Diseases / Q000235:genetics; D010812:Physical Stimulation; D012038:Regeneration / Q000235:genetics / Q000502:physiology; D013314:Stress, Mechanical
chemical_list: C486115:Fgf6 protein, mouse; D051522:Fibroblast Growth Factor 6