title: Quantum dots with phenylboronic acid tags for specific labeling of sialic acids on living cells.
abstract: Sialic acids with a nine-carbon backbone are commonly found at the terminal position of the glycans structures on cell membranes. The unique distribution and ubiquitous existence of sialic acid on the cell membrane make them important mediators in various biological and pathological processes. We report a new class of imaging probes based on semiconductor quantum dots with small molecular phenylboronic acid tags for highly specific and efficient labeling of sialic acid on living cells. Our results have shown that the use of these probes enables one-step labeling and continuous tracking of the cell surface sialic acid moieties without any pretreatment of living cells. The one-step procedure with fast binding kinetics and the biocompatibility of these probes make it an ideal noninvasive technology for living cell imaging. We also find that the labeled sialic acids undergo quick internalization shortly after surface binding via endocytosis and eventually distribute in the perinuclear region. This distribution pattern is consistent with the notion that sialylated glycoproteins are populated on cell membranes and recycled through the vesicular exocytotic and endocytic pathways. The superior photostability and brightness of quantum dots enable quantitative analysis of the diffusion dynamics of sialic acids, which has been a significant challenge for glycan imaging.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Aiping
authors.initials: A
authors.lastname: Liu
authors.forename: Shuo
authors.initials: S
authors.lastname: Peng
authors.forename: Jian Chow
authors.initials: JC
authors.lastname: Soo
authors.forename: Min
authors.initials: M
authors.lastname: Kuang
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Hongwei
authors.initials: H
authors.lastname: Duan
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002470:Cell Survival; D008024:Ligands; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D016716:PC12 Cells; D045663:Quantum Dots; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*
chemical_list: D001897:Boronic Acids; D008024:Ligands; D012794:Sialic Acids