title: [Effect of rhIGF-1 on cytochrome C and caspase-3 expression in neonatal rats with hypoxic-ischemic brain damage in vivo].
abstract: OBJECTIVE
  To study the effect of rhIGF-1on the mRNA and protein expression of cytochrome C (Cyt-C) and caspase-3 in neonatal rats with hypoxic-ischemic brain damage (HIBD).
  
  
  METHODS
  Ninety neonatal Sprague-Dawley rats were randomly divided into three groups: normal control, HIBD, and HIBD+rhIGF-1 (rhIGF-1 was given intraperitoneally right after HI). Rat HIBD model was prepared according the Rice-Vannucci method. RT-PCR and Western blot methods were used to measure the mRNA and protein expression of Cyt-C and caspase-3 24, 48 and 72 hrs after HI (n=10 each time point).
  
  
  RESULTS
  At all time points, both Cyt-C mRNA and caspase-3 mRNA expression levels in the HIBD group increased compared with those in the normal control group, and those in the HIBD+rhIGF-1 group also increased compared with that in the normal control group but decreased compared with that in the HIBD group. There were statistical significances among the three groups (P<0.01). At all time points, the changes of both Cyt-C and caspase-3 protein expression in the three groups were similar to those of the mRNA expression: both Cyt-C and caspase-3 protein expression levels increased in the HIBD group compared with those in the normal control group, and those in the HIBD+rhIGF-1 group also increased compared with those in the normal control group but decreased compared with those in the HIBD group. There were statistical significances among the three groups (P<0.01).Pearson correlation analysis showed that mRNA and protein expression of Cyt-C were positively correlated to casapse-3 mRNA and protein expression in the HIBD and the HIBD+rhIGF-1 groups.
  
  
  CONCLUSIONS
  rhIGF-1 may inhibit the Cyt-C release and caspase-3 expression, and thus provides neuroprotection against HIBD in neonatal rats.
authors.affiliation: Department of Pediatrics, Third Xiangya Hospital, Central South University, Changsha 410013, China. Email: heiming_yan@yahoo.com.cn.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Hei
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Huang
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D045304:Cytochromes c; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D045304:Cytochromes c; D053148:Caspase 3