title: The role of nuclear factor-kappa-B p50 subunit in the development of endometriosis.
abstract: p50 is a member of the NF-kappaB family known to be involved in endometriosis. To gain insight into the roles of p50 in the development of endometriosis, we cross-transplanted endometrial fragments from p50 knockout mice to wild-type mice and vice versa, and also autotransplanted the fragments within the knockout and wild-type mice, inducing endometriosis. We then evaluated the size of the endometrial implants, and immunoreactivity to phosphorylated p65 (p-p65), PKCepsilon and TRPV1 in ectopic and eutopic endometrium as well as in vagina. We found that p50 deletion significantly reduces the size of endometrial implants. The immunoreactivity to p-p65 and PKCepsilon, but not TRPV1, was reduced in endometrial implants in p50 knockout mice. Deletion of p50 significantly reduced p-p65 and PKCepsilon, but not TRPV1, expression in eutopic endometrium and vagina. It also disrupts NF-kappaB activation and PKCepsilon expression in eutopic and vagina, suggesting the role of NF-kappaB in regulating PKCepsilon, which plays an important role in nociception. These data show that p50 is involved in the development of endometriosis and may be a promising therapeutic target.
authors.affiliation: Shanghai Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Qunyan
authors.initials: Q
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xishi
authors.initials: X
authors.lastname: Liu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Geng
authors.forename: Sun-Wei
authors.initials: SW
authors.lastname: Guo
mesh_terms: D000818:Animals; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism*; D051744:Protein Kinase C-epsilon / Q000276:immunology / Q000378:metabolism; D018709:Statistics, Nonparametric; D050916:TRPV Cation Channels / Q000378:metabolism; D051996:Transcription Factor RelA / Q000276:immunology / Q000378:metabolism
chemical_list: D052002:NF-kappa B p50 Subunit; C498144:Rela protein, mouse; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D051996:Transcription Factor RelA; D051744:Protein Kinase C-epsilon