title: [1, 25-Dihydroxyvitamin D3 increases in vitro vascular calcification through calcified blood vessel cells].
abstract: OBJECTIVE
  Accumulated evidence suggest that the development of vascular calcification is similar to osteogenesis. Here we want to elucidate the effect of the common used osteo-regulatory factor 1,25(OH)2D3 on vascular calcification.
  
  
  RESULTS
  Adding 10(-9) mol/L to the culture media 1,25(OH)2D3 time dependently increased the calcium deposition on the in vitro calcification of bovine vascular smooth muscle cells (BVSMCs) induced by beta-GP. It also increased cellular alkaline phosphatase activity by 301.1% during the calcified process. Osteocalcin, one of the osteogenic specific metric proteins, was dramatically elevated by 58.3% during the calcified processes, which indicate the transformation of BVSMCs to osteoblastic cell. 1,25(OH)2D3 had no such effect on non-calcified BVSMCs.
  
  
  CONCLUSIONS
  These data suggest that 1,25(OH)2D3 exerts a stimulatory effect on vascular calcification through increasing the synthesis of ALP. This effect shares the same character as osteoblast cells. This effect is limited to the calcified prone vascular cell.
authors.affiliation: Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Wang
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Wang
authors.forename: Wan-Ren
authors.initials: WR
authors.lastname: Tong
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015675:Osteocalcin / Q000378:metabolism; D061205:Vascular Calcification / Q000378:metabolism* / Q000473:pathology; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C118756:dihydroxy-vitamin D3; D015675:Osteocalcin; D014807:Vitamin D; D002117:Calcitriol