title: Lentinus (Panus) tigrinus augmentation of a historically contaminated soil: matrix decontamination and structure and function of the resident bacterial community.
abstract: The ability of Lentinus tigrinus to grow and to degrade persistent aromatic hydrocarbons in aged contaminated soil was assessed in this study. L. tigrinus extensively colonized the soil; its degradation activity after 60 d incubation at 28°C, however, was mostly limited to dichloroaniline isomers, polychlorinated benzenes and diphenyl ether while the fungus was unable to deplete 9,10-anthracenedione and 7-H-benz[DE]anthracene-7-one which were the major soil contaminants. Although clean-up levels were limited, both density of cultivable heterotrophic bacteria and richness of the resident bacterial community in L. tigrinus microcosms (LtM) increased over time to a significantly larger extent than the respective amended incubation controls (1.9×10(9) CFU g(-1) vs. 1.0×10(9) CFU g(-1) and 37 vs. 16, respectively). Naphthalene- and catechol 2,3-dioxygenase gene copy numbers, however, decreased over time at a higher rate in LtM than in incubation controls likely due to a higher stimulation on heterotrophs than xenobiotics-degrading community members.
authors.affiliation: Dipartimento di Biologia Cellulare e Ambientale, University of Perugia, Via del Giochetto 06100 Perugia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Federici
authors.forename: M A
authors.initials: MA
authors.lastname: Giubilei
authors.forename: T
authors.initials: T
authors.lastname: Cajthaml
authors.forename: M
authors.initials: M
authors.lastname: Petruccioli
authors.forename: A
authors.initials: A
authors.lastname: D'Annibale
mesh_terms: D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D003666:Decontamination; D005227:Fatty Acids / Q000032:analysis; D018628:Gene Dosage; D020074:Lentinula / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D019216:Metals, Heavy / Q000032:analysis; D025282:Mycelium / Q000254:growth & development; D010743:Phospholipids / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D004271:DNA, Fungal; D005227:Fatty Acids; D019216:Metals, Heavy; D010743:Phospholipids; D012987:Soil; D012989:Soil Pollutants; D050557:Catechol 2,3-Dioxygenase