title: Hollow fiber-based liquid phase microextraction (HF-LPME) for a highly sensitive HPLC determination of sulfonamides and their main metabolites.
abstract: In this paper, three phase-hollow fiber-based liquid phase microextraction (HF-LPME) combined with a HPLC procedure using diode array (DAD) and fluorescence detection (FLD) has been developed for the determination of four widely used sulfonamides: sulfadiazine, sulfamerazine, sulfamethazine, sulfamethoxazole and their main metabolites, the corresponding N(4)-acetyl derivatives: N(4)-acetyl-sulfadiazine, N(4)-acetyl-sulfamerazine, N(4)-acetyl-sulfamethazine, N(4)-acetyl-sulfamethoxazole. A Q3/2 Accurel KM polypropylene hollow fiber supporting 1-octanol was used between a 2 M Na(2)SO(4) aqueous solution (pH 4) as a donor phase and aqueous solution (pH 12) as an acceptor phase. The procedure allows very low detection and quantitation limits of 0.3-33 ng L(-1) and 0.9-100 ng L(-1), respectively. The proposed method was applied to the determination of the analytes in environmental water samples (surface, tap and wastewater).
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, 41012 Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Ramos Payán
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Bello López
authors.forename: Rut
authors.initials: R
authors.lastname: Fernández-Torres
authors.forename: Mercedes
authors.initials: M
authors.lastname: Villar Navarro
authors.forename: Manuel
authors.initials: M
authors.lastname: Callejón Mochón
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016018:Least-Squares Analysis; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D013449:Sulfonamides; D014874:Water Pollutants, Chemical