title: Casbane diterpene as a promising natural antimicrobial agent against biofilm-associated infections.
abstract: Croton nepetaefolius is a native plant from northeastern Brazil that belongs to the Euphorbiaceae family. The biological action of this plant has been extensively explored, being the secondary metabolites responsible for its properties alkaloids, diterpenes, and triterpenes. This study aimed to evaluate the ability of casbane diterpene (CD), isolated from the ethanolic extract of C. nepetaefolius, to inhibit microbial growth and biofilm formation of several clinical relevant species (bacteria and yeasts). It was found that CD possessed biocidal and biostatic activity against the majority of the species screened, with minimal active concentrations ranging between 125 and 500 µg/mL. In addition, it was observed that biofilm formation was inhibited even when the planktonic growth was not significantly affected. In conclusion, CD showed potential to be a natural tool for the treatment of diseases caused by different infectious microorganisms.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE, Brazil. victorcarneiro@ufc.br
authors.forename: Victor Alves
authors.initials: VA
authors.lastname: Carneiro
authors.forename: Hélcio Silva Dos
authors.initials: HS
authors.lastname: Santos
authors.forename: Francisco Vassiliepe Sousa
authors.initials: FV
authors.lastname: Arruda
authors.forename: Paulo Nogueira
authors.initials: PN
authors.lastname: Bandeira
authors.forename: Maria Rose Jane Ribeiro
authors.initials: MR
authors.lastname: Albuquerque
authors.forename: Maria Olívia
authors.initials: MO
authors.lastname: Pereira
authors.forename: Mariana
authors.initials: M
authors.lastname: Henriques
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000517:prevention & control; D018441:Biofilms; D029802:Croton / Q000737:chemistry; D004224:Diterpenes / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects; D009181:Mycoses / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D004224:Diterpenes; D010936:Plant Extracts