title: [A primary study of immunotherapy with carcinoembryonic antigen peptide-pulsed, autologous human cultured dendritic cells in patients with advanced non-small cell lung cancer].
abstract: BACKGROUND
  Dendritic cell (DC)-based immunotherapy is a new approach and effective for some malignant tumors. The aim of this study is to observe the efficacy and toxicity of immunotherapy with carcinoembryonic antigen (CEA) peptide-pulsed DCs in patients with refractory advanced lung cancer.
  
  
  METHODS
  Lung cancer patients with high CEA expression were enrolled into this project. Autologous DCs were generated from patients' plastic-adherent peripheral blood mononuclear cells and loaded with CEA 5 days later. Cytokine-induced killer cells (CIK) were cultured from non-adherent peripheral blood mononuclear cells. DCs and CIK were transfused to patients. Responses and toxicities were observed.
  
  
  RESULTS
  A total of 22 patients with lung cancer received DCs immunotherapy. DCs doses were 2.5×10⁶-9.6×10⁷ (5.03×10⁶). CIK doses were 3.4×10⁸-46×10⁸. CD3, CD8, NK and IFN-γ levels obviously increased after treatment (P < 0.05). The 1-year survival rate was 68.2% (15/22). Main toxicities were fever and rash.
  
  
  CONCLUSIONS
  DCs-based immunotherapy is feasible and safe to patients with lung cancer.
authors.affiliation: Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai 200030, P.R.China.
authors.forename: Baohui
authors.initials: B
authors.lastname: Han
authors.forename: Hua
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authors.lastname: Zhong
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fan
authors.forename: Guangli
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authors.lastname: Feng
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Leluo
authors.initials: L
authors.lastname: Gong
authors.forename: Tianqing
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authors.lastname: Chu
authors.forename: Wei
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authors.lastname: Zhang
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authors.lastname: Jin
authors.forename: Chunlei
authors.initials: C
authors.lastname: Shi
authors.forename: Yizhuo
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authors.lastname: Zhao
authors.forename: Huifang
authors.initials: H
authors.lastname: Sha
authors.forename: Qianggang
authors.initials: Q
authors.lastname: Dong
authors.forename: Meilin
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authors.lastname: Liao