title: Microbial and bioconversion production of D-xylitol and its detection and application.
abstract: D-Xylitol is found in low content as a natural constituent of many fruits and vegetables. It is a five-carbon sugar polyol and has been used as a food additive and sweetening agent to replace sucrose, especially for non-insulin dependent diabetics. It has multiple beneficial health effects, such as the prevention of dental caries, and acute otitis media. In industry, it has been produced by chemical reduction of D-xylose mainly from photosynthetic biomass hydrolysates. As an alternative method of chemical reduction, biosynthesis of D-xylitol has been focused on the metabolically engineered Saccharomyces cerevisiae and Candida strains. In order to detect D-xylitol in the production processes, several detection methods have been established, such as gas chromatography (GC)-based methods, high performance liquid chromatography (HPLC)-based methods, LC-MS methods, and capillary electrophoresis methods (CE). The advantages and disadvantages of these methods are compared in this review.
authors.affiliation: Biorefining Research Initiative and Department of Biology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Zi-Hua
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Sanfeng
authors.initials: S
authors.lastname: Chen
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qin
mesh_terms: D002175:Candida / Q000378:metabolism; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014993:Xylitol / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014993:Xylitol