title: Studies of dimethylglycine oxidase isoenzymes in Arthrobacter globiformis cells.
abstract: Glycine betaine (GB) could be used by Arthrobacter globiformis cells as a sole carbon source. The cells took up this molecule in the low as well as in the high salinity medium. Addition of GB to the mineral medium with high salt concentration revealed that GB was also used as an osmoprotectant. Dimethylglycine oxidase (DMGO) was involved in the catabolism of GB. Two genes for DMGO were detected in a cloned 26267 bp fragment of A. globiformis DNA. The genes involved in the tetrahydrofolate-dependent assimilation of methyl groups were located nearby the two of DMGO genes. Both cloned A. globiformis DMGO were active. The activity of DMGO was detected in A. globiformis cells and it depended on the addition of GB and the salinity of the medium. Reverse transcription-PCR demonstrated that the addition of GB influenced the transcription of dmg genes.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania. vida.casaite@bchi.lt
authors.forename: Vida
authors.initials: V
authors.lastname: Casaitė
authors.forename: Simona
authors.initials: S
authors.lastname: Povilonienė
authors.forename: Rita
authors.initials: R
authors.lastname: Meškienė
authors.forename: Rasa
authors.initials: R
authors.lastname: Rutkienė
authors.forename: Rolandas
authors.initials: R
authors.lastname: Meškys
mesh_terms: D001173:Arthrobacter / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007527:Isoenzymes; D001622:Betaine; D000587:Oxidoreductases Acting on CH-NH Group Donors; C431689:dimethylglycine oxidase