title: Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60.
abstract: This study explored the effects of nuclear factor-kappa B (NF-κB) inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P<0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P>0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly higher than in the controls (P<0.05). Therefore, we conclude that Bay 11-7082 can enhance Fas-mediated apoptosis in HL-60 cells by downregulating FasL and XIAP levels.
authors.affiliation: Department of Hematology, The Central Hospital of Wuhan, and Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. wlmarx96@yahoo.com.cn
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Shi
authors.initials: S
authors.lastname: Zhao
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D053222:Fas Ligand Protein / Q000187:drug effects / Q000502:physiology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology; D018922:HL-60 Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000502:physiology; D009570:Nitriles / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013450:Sulfones / Q000494:pharmacology; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000502:physiology; D019014:fas Receptor / Q000187:drug effects / Q000502:physiology*
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; D051636:X-Linked Inhibitor of Apoptosis Protein; D019014:fas Receptor