title: [Study on expression of ELAM-1 and ICAM-1 mRNA on microvascular endothelial cells during focal cerebral ischemia/reperfusion].
abstract: OBJECTIVE
  To evaluate the role of ELAM-1 and ICAM-1 in the course of inflammatory reactions during focal brain ischemia/reperfusion.
  
  
  METHODS
  The focal brain ischemia/reperfusion model is carried by occluding middle cerebral artery. The expression of ELAM-1 and ICAM-1 mRNA after ischemia/reperfusion was evaluated with RT-PCR.
  
  
  RESULTS
  No ELAM-1 and ICAM-1 mRNA were detected in the sham-operated cortex and only little in the nonischemic cortex. The expression of ELAM-1 and ICAM-1 mRNA were upregulated at 1 hour, peaked at 6 hour and 3 hour respectively and remained elevated for up to 48 hours after ischemia/reperfusion.
  
  
  CONCLUSIONS
  ELAM-1 and ICAM-1 participate in brain injury during focal ischemia/reperfusion and both of them play an important role in leukocyte infiltration into the ischemic tissues.
authors.affiliation: Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Wang
authors.forename: S P
authors.initials: SP
authors.lastname: Cai
authors.forename: J
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism; D019040:E-Selectin / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D019040:E-Selectin; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1