title: [Construction and experimental study on off-line hybrid bioartificial liver supporting system with human liver cell line].
abstract: OBJECTIVE
  To construct an off-line hybrid bioartificial liver supporting system with human liver cell line, and study it's effect on the plasma from patients with liver failure.
  
  
  METHODS
  We established the bioreactor using Psu-2s (Fresenius) cultured with Hep G2 cell transfected with human augmenter of liver regeneration (hALR) gene, then constructed a hybrid bioartificial liver supporting system, at last using the bioartificial liver support system to purify the plasma treated 2 hours with serum bilirubin absorbent, separated from acute on chronic liver failure patients infected by hepatitis B virus.
  
  
  RESULTS
  Bioreactor was successful constructed. The cell viability in perigastrum of bioreactor is 85.2% and cell propagated rapidly. Before and after treating with bilirubin absorbent, serum total bilirubin was (176.19 +/- 54.14) micromol/L and (50.1 +/- 16.85) micromol/L respectively (P = 0.0002). While there were no significance difference in the level of albumin, urea and glucose. At the begin and end of treatment with bioartificial liver, serum total bilirubin was (50.10 +/- 16.85) micromol/L and (30.27 +/- 15.02) micromol/L respectively (P = 0.000), the urea and albumin increased, urea has significantly difference, but the change of albumin hasn't.
  
  
  CONCLUSIONS
  The off-line hybrid bioartificial liver supporting system with human liver cell line were builded successfully and have synthesis and metabolism functions for acute on chronic liver failure patients.
authors.affiliation: 302 Hospital of the PLA, Beijing 100039, China.
authors.forename: Yi-Hui
authors.initials: YH
authors.lastname: Rong
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Liu
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: You
authors.forename: Wan-Shu
authors.initials: WS
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Pan-Yong
authors.initials: PY
authors.lastname: Mao
authors.forename: Shao-Jie
authors.initials: SJ
authors.lastname: Xin
mesh_terms: D000328:Adult; D016477:Artifacts; D001663:Bilirubin / Q000378:metabolism*; D019149:Bioreactors / Q000592:standards; D002678:Chimera; D058625:End Stage Liver Disease / Q000503:physiopathology*; D022781:Hepatocytes / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000502:physiology*; D017093:Liver Failure; D019164:Liver, Artificial / Q000706:statistics & numerical data*; D008297:Male; D008875:Middle Aged
chemical_list: D001663:Bilirubin