title: Modeling human endometrial decidualization from the interaction between proteome and secretome.
abstract: BACKGROUND
  Decidualization of the human endometrium, which involves morphological and biochemical modifications of the endometrial stromal cells (ESCs), is a prerequisite for adequate trophoblast invasion and placenta formation.
  
  
  OBJECTIVE
  This study aims to investigate the proteome and secretome of in vitro decidualized ESCs. These data were combined with published genomic information and integrated to model the human decidualization interactome.
  
  
  METHODS
  Prospective experimental case-control study.
  
  
  METHODS
  A private research foundation.
  
  
  METHODS
  Sixteen healthy volunteer ovum donors.
  
  
  METHODS
  Endometrial samples were obtained, and ESCs were isolated and decidualized in vitro.
  
  
  METHODS
  Two-dimensional difference in-gel electrophoresis, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry, Western blot, human protein cytokine array, ELISA, and bioinformatics analysis were performed.
  
  
  RESULTS
  The proteomic analysis revealed 60 differentially expressed proteins (36 over- and 24 underexpressed) in decidualized versus control ESCs, including known decidualization markers (cathepsin B) and new biomarkers (transglutaminase 2, peroxiredoxin 4, and the ACTB protein). In the secretomic analysis, a total of 13 secreted proteins (11 up- and 2 down-regulated) were identified, including well-recognized markers (IGF binding protein-1 and prolactin) and novel ones (myeloid progenitor inhibitory factor-1 and platelet endothelial cell adhesion molecule-1). These proteome/secretome profiles have been integrated into a decidualization interactome model.
  
  
  CONCLUSIONS
  Proteomic and secretomic have been used as hypothesis-free approaches together with complex bioinformatics to model the human decidual interactome for the first time. We confirm previous knowledge, describe new molecules, and we have built up a model for human in vitro decidualization as invaluable tool for the diagnosis, therapy, and interpretation of biological phenomena.
authors.affiliation: Fundación IVI-Instituto Universitario IVI-Universidad de Valencia, INCLIVA Valencia 46015, Spain.
authors.forename: Tamara
authors.initials: T
authors.lastname: Garrido-Gomez
authors.forename: Francisco
authors.initials: F
authors.lastname: Dominguez
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Lopez
authors.forename: Emilio
authors.initials: E
authors.lastname: Camafeita
authors.forename: Alicia
authors.initials: A
authors.lastname: Quiñonero
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Martinez-Conejero
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
authors.forename: Ana
authors.initials: A
authors.lastname: Conesa
authors.forename: Carlos
authors.initials: C
authors.lastname: Simón
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016022:Case-Control Studies; D048430:Cell Shape; D002478:Cells, Cultured; D019295:Computational Biology; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000378:metabolism; D003656:Decidua / Q000166:cytology / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000166:cytology / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008954:Models, Biological; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000235:genetics*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017154:Stromal Cells / Q000502:physiology / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D015415:Biomarkers; D017077:Culture Media, Conditioned; D016207:Cytokines; D020543:Proteome