title: Abbott RealTime HIV-1 m2000rt viral load testing: manual extraction versus the automated m2000sp extraction.
abstract: The Abbott RealTime HIV-1 assay is a real-time nucleic acid amplification assay available for HIV-1 viral load quantitation. The assay has a platform for automated extraction of viral RNA from plasma or dried blood spot samples, and an amplification platform with real time fluorescent detection. Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually.
authors.affiliation: University of the Witwatersrand, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Science, 7 York Rd Parktown, Johannesburg 2000, South Africa. lesley.scott@nhls.ac.za
authors.forename: Lesley E
authors.initials: LE
authors.lastname: Scott
authors.forename: John A
authors.initials: JA
authors.lastname: Crump
authors.forename: Emma
authors.initials: E
authors.lastname: Msuya
authors.forename: Anne B
authors.initials: AB
authors.lastname: Morrissey
authors.forename: Willem F
authors.initials: WF
authors.lastname: Venter
authors.forename: Wendy S
authors.initials: WS
authors.lastname: Stevens
mesh_terms: D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load / Q000379:methods*
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic