title: Metallothionein-2A overexpression increases the expression of matrix metalloproteinase-9 and invasion of breast cancer cells.
abstract: The overexpression of metallothionein-2A (MT-2A) is frequently observed in invasive human breast tumors and has been linked with more aggressive breast cancers. MT-2A overexpression led to the induction of MDA-MB-231 breast cancer cell migratory and invasive abilities. The reduction of MT-2A expression through small interfering RNA (siRNA) targeting MT-2A in invasive MDA-MB-231 cells completely inhibited both cell invasion and migration. In addition, the expression of matrix metalloproteinase-9 (MMP-9) and the transcriptional activity of AP-1 and NF-κB were upregulated by MT-2A overexpression. Collectively, our results provide the first demonstration that MT-2A promotes breast cancer cell invasion by upregulating MMP-9 via AP-1 and NF-κB activation. Furthermore, we found that MT-2A silencing can inhibit breast cancer invasiveness.
authors.affiliation: Department of Toxicology, College of Pharmacy, Chungnam National University, Yuseong-Gu, Daejeon, South Korea.
authors.forename: Hyung Gyun
authors.initials: HG
authors.lastname: Kim
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Han
authors.forename: Yong Pil
authors.initials: YP
authors.lastname: Hwang
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Bong Hwan
authors.initials: BH
authors.lastname: Park
authors.forename: Hye Gwang
authors.initials: HG
authors.lastname: Jeong
mesh_terms: D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics* / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics*; D034741:RNA, Small Interfering / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D034741:RNA, Small Interfering; D018808:Transcription Factor AP-1; D008668:Metallothionein; D020780:Matrix Metalloproteinase 9