title: Virus-like particles in Eimeria tenella are associated with multiple RNA segments.
abstract: Total nucleic acids from sporulated oocysts of Eimeria tenella isolated from Changchun in China were found to contain three extrachromosomal double-stranded RNA segments (dsRNAs) of 1.4, 2.4 and 3.6 kb in sizes. These RNAs were resistant to RNase A digestion under high salt concentration (0.3 M NaCl). RNA-dependent RNA polymerase (RDRP) activity was detected in crude extracts of E. tenella sporulated oocysts containing these nucleic acid species. Virus-like particles (VLPs) were shown to have a diameter of approximately 38 nm under Electron Microscopy (EM) after purification by sucrose density gradient centrifugation. In keeping with the nomenclature generally adopted for protozoan viruses, we have named this isolate as E. tenella virus (ETV) which is the first virus isolated from E. tenella.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun 130062, China.
authors.forename: Qianzhong
authors.initials: Q
authors.lastname: Han
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Jinhong
authors.initials: J
authors.lastname: Gai
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D016786:Eimeria tenella / Q000235:genetics / Q000821:virology*; D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D033661:Oocysts / Q000821:virology; D011201:Poultry Diseases / Q000469:parasitology; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D014771:Virion / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase