title: Rapid functional screening of effective siRNAs against Plk1 and its growth inhibitory effects in laryngeal carcinoma cells.
abstract: Plk 1 is overexpressed in many human malignancies including laryngeal carcinoma. However, its therapeutic potential has been never examined in laryngeal carcinoma. In the present study, a simple cellular morphology-based strategy was firstly proposed for rapidly screening the effective siRNAs against Plk1. Furthermore, we investigated the effects of Plk1 depletion via a novel identified effective siRNA against Plk1, Plk1 siRNA-607, on human laryngeal carcinoma Hep-2 cells. The results indicated that Plk1 siRNA-607 transfection resulted in a significant inhibition in Plk1 expression in cells, and subsequently caused a dramatic mitotic cell cycle arrest followed by massive apoptotic cell death, and eventually resulted in a significant decrease in growth and viability of the laryngeal carcinoma cells. Taken together, our present study not only suggests a simple strategy for rapidly screening effective siRNAs against Plk1 but also implicates that Plk1 may serve as a potential therapeutic target in human laryngeal carcinoma.
authors.affiliation: Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Lan
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Qing
authors.initials: Q
authors.lastname: Ai
authors.forename: Zhengmei
authors.initials: Z
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Ji
authors.forename: Suling
authors.initials: S
authors.lastname: Hong
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Song
authors.forename: Youquan
authors.initials: Y
authors.lastname: Bu
mesh_terms: D017209:Apoptosis; D002277:Carcinoma / Q000628:therapy*; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007822:Laryngeal Neoplasms / Q000628:therapy*; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism* / Q000627:therapeutic use; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D000097869:Polo-Like Kinase 1