title: Solid-state fermentation of agro-industrial wastes to produce bioorganic fertilizer for the biocontrol of Fusarium wilt of cucumber in continuously cropped soil.
abstract: Agro-industrial wastes of cattle dung, vinegar-production residue and rice straw were solid-state fermented by inoculation with Trichoderma harzianum SQR-T037 (SQR-T037) for production of bioorganic fertilizers containing SQR-T037 and 6-pentyl-α-pyrone (6PAP) to control Fusarium wilt of cucumber in a continuously cropped soil. Fermentation days, temperature, inoculum and vinegar-production residue demonstrated significant effects on the SQR-T037 biomass and the yield of 6PAP, based on fractional factorial design. Three optimum conditions for producing the maximum SQR-T037 biomass and 6PAP yield were predicted by central composite design and validated. Bioorganic fertilizer containing 8.46 log(10) ITS copies g(-1) dry weight of SQR-T037 and 1291.73 mg kg(-1) dry weight of 6PAP, and having the highest (p<0.05) biocontrol efficacy, was achieved at 36.7 fermentation days, 25.9°C temperature, 7.6% inoculum content, 41.0% vinegar-production residue, 20.0% rice straw and 39.0% cattle dung. This is a way to offer a high value-added use for agro-industrial wastes.
authors.affiliation: Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Chen
authors.forename: Xingming
authors.initials: X
authors.lastname: Yang
authors.forename: Waseem
authors.initials: W
authors.lastname: Raza
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.forename: Fengge
authors.initials: F
authors.lastname: Zhang
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000383:Agriculture / Q000379:methods*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D018553:Cucumis sativus / Q000382:microbiology*; D005285:Fermentation; D005308:Fertilizers; D005670:Fusarium / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007700:Kinetics; D010935:Plant Diseases / Q000382:microbiology; D011753:Pyrones / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D005308:Fertilizers; D007220:Industrial Waste; D011753:Pyrones; D014867:Water; D019342:Acetic Acid