title: Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys.
abstract: During acute rejection, numerous pro-inflammatory and cytotoxic monocytes accumulate in the vasculature of experimental renal allografts. Arrestins (ARRBs) are cellular regulators of inflammation, but nothing is known about their expression during rejection. Intravascular mononuclear graft leukocytes were isolated 4 days after kidney transplantation. ARRB1 and ARRB2 mRNA expression was reduced in blood leukocytes from allografts undergoing acute rejection, whereas on the protein level only ARRB2 was changed. Flow cytometry and confocal microscopy revealed ARRB1 and ARRB2 expression by monocytes and T cells, with a selective decrease in ARRB2 expression in monocytes during acute rejection. I-κB directly interacted with ARRB2 and the levels of both proteins strongly correlated. Concomitantly, the mRNA expression of NF-κB targeted genes increased. Our results suggest that activation of blood monocytes in renal isografts is dampened by high ARRB2 levels. During acute rejection, ARRB2 levels are reduced and classical monocyte activation is enabled via NF-κB activation.
authors.affiliation: Laboratory of Experimental Surgery, Department of General and Thoracic Surgery, Justus-Liebig-University Giessen, Rudolf-Buchheim-Strasse 7, Giessen, Germany. Anna.Zakrzewicz@chiru.med.uni-giessen.de
authors.forename: Anna
authors.initials: A
authors.lastname: Zakrzewicz
authors.forename: Gabriela
authors.initials: G
authors.lastname: Krasteva
authors.forename: Jochen
authors.initials: J
authors.lastname: Wilhelm
authors.forename: Hartmut
authors.initials: H
authors.lastname: Dietrich
authors.forename: Sigrid
authors.initials: S
authors.lastname: Wilker
authors.forename: Winfried
authors.initials: W
authors.lastname: Padberg
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Wygrecka
authors.forename: Veronika
authors.initials: V
authors.lastname: Grau
mesh_terms: D000208:Acute Disease; D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002469:Cell Separation; D015536:Down-Regulation / Q000276:immunology; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D023421:Models, Animal; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014184:Transplantation, Homologous / Q000473:pathology*; D000071559:beta-Arrestin 1; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins
chemical_list: C000605253:ARRB1 protein, human; C000605254:ARRB2 protein, human; C000605256:Arrb1 protein, rat; C000605257:Arrb2 protein, rat; D019390:Arrestins; D000071559:beta-Arrestin 1; D000071560:beta-Arrestin 2; D000071557:beta-Arrestins