title: Effects of chitosan-carboxymethyl dextran nanoparticles on cell proliferation and on serum cytokine regulation.
abstract: The bioactivity of chitosan-carboxymethyl dextran nanoparticles (CDNP) made using chitosans with different degrees of deacetylation (DA) (CDNP98: CDNP made with 98% DA chitosan; CDNP78: CDNP made with 78% DA chitosan) were tested by analysis of their in vitro effects on HeLa tumor cell and mouse fibroblast proliferation, and their in vivo effects on the regulation of mouse serum cytokine levels. CDNPs (50 to 100 microg/ml) induced higher fibroblast proliferation than chitosans (CDNP98 > CDNP78 > chitosan), and were also more effective than chitosans in inhibition of HeLa cell proliferation in a dose-dependent manner (25 to 100 microg/ml) (CDNP78 > CDNP98 > chitosans). CDNPs also induced a higher serum IL-5 level than chitosans (CDNP98 > CDNP78 > chitosans) within 3 h, and CDNP78 had a greater impact on IL-6 regulation but regulated IL-10 over a shorter duration than CDNP98 within the first 3 h. Combined with their ability to reduce serum IL-17 at 24 h, these biocompatible, immuno-active CDNPs demonstrated the potential to be applied as biomedical materials.
authors.affiliation: United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Lin
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Saburo
authors.initials: S
authors.lastname: Minami
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D048271:Chitosan / Q000494:pharmacology*; D016207:Cytokines / Q000097:blood*; D003911:Dextrans / Q000494:pharmacology*; D004337:Drug Carriers / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage*
chemical_list: D016207:Cytokines; D003911:Dextrans; D004337:Drug Carriers; D048271:Chitosan