title: [Construction and identification of procaryotic expression vector of human NIT1 gene].
abstract: BACKGROUND
  The human FHIT gene at chromosome 3p14.2 is a tumor suppressor gene, and its abnormality in structure and function is related to carcinogenesis and progression of lung cancer. It is postulated that FHIT and NIT1 likewise collaborate in biochemical or cellular pathway in mammalian cells, but their molecular mechanisms in lung cancer cell are still unknown. The aim of this study is to construct procaryotic expression vector of human NIT1, providing a foundation to explore the expression of human NIT1 protein and to study the interaction of NIT1 and FHIT genes in lung cancer cell lines.
  
  
  METHODS
  The NIT1 cDNA was acquired by RT-PCR. EcoRI/NotI digested PCR product was directly cloned into procaryotic expression vector pET-32a.
  
  
  RESULTS
  The sequence of NIT1 cDNA clone exactly corresponded with the sequence of NIT1 cDNA in GenBank. The expectant fragments of DNA were obtained after recombinant procaryotic expression vector was digested by EcoRI and NotI.
  
  
  CONCLUSIONS
  The procaryotic expression vector of human NIT1 is successfully constructed by RT-PCR and direct clone. It provides an important basis to detect the expression of NIT1 protein and to further explore the relationship between NIT1 and FHIT genes in oncogenesis and development of human lung cancer.
authors.affiliation: Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
authors.forename: Hanlin
authors.initials: H
authors.lastname: Wu
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Guowei
authors.initials: G
authors.lastname: Che
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Chen
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Sun