title: A simple and inexpensive automated technique for measurement of serum nitrite/nitrate.
abstract: OBJECTIVE
  We described an automated technique for measurement of serum nitrite/nitrate (NO(x)) using the Cobas Mira clinical chemistry analyzer.
  
  
  METHODS
  NO(x) was measured by the modified Griess method. Precision, accuracy, linearity, instrument carry-over and lower limit of quantitation (LLOQ) were assessed.
  
  
  RESULTS
  The automated technique for measurement of serum NO(x) was linear, precise, and accurate. It has a LLOQ of 2.0 μmol/L.
  
  
  CONCLUSIONS
  Serum NO(x) measured by the modified Griess method can be applied easily to the Cobas Mira clinical chemistry analyzer.
authors.affiliation: Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Etiane
authors.initials: E
authors.lastname: Tatsch
authors.forename: Guilherme Vargas
authors.initials: GV
authors.lastname: Bochi
authors.forename: Renata da Silva
authors.initials: Rda S
authors.lastname: Pereira
authors.forename: Helena
authors.initials: H
authors.lastname: Kober
authors.forename: Vanessa Albertina
authors.initials: VA
authors.lastname: Agertt
authors.forename: Marli Matiko Anraku
authors.initials: MM
authors.lastname: de Campos
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gomes
authors.forename: Marta Maria Medeiros Frescura
authors.initials: MM
authors.lastname: Duarte
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D057205:Automation, Laboratory; D001774:Blood Chemical Analysis / Q000191:economics / Q000379:methods*; D002624:Chemistry, Clinical / Q000191:economics / Q000379:methods*; D005029:Ethylenediamines / Q000737:chemistry; D006801:Humans; D009566:Nitrates / Q000097:blood*; D009573:Nitrites / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry; D013424:Sulfanilamides / Q000737:chemistry
chemical_list: D005029:Ethylenediamines; C095000:Griess reagent; D009566:Nitrates; D009573:Nitrites; D013424:Sulfanilamides