title: Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150.
abstract: The indica rice cultivar Xiangzi 3150 (XZ3150) confers a high level of resistance to 95% of the isolates of Magnaporthe oryzae (the agent of rice blast disease) collected in Hunan Province, China. To identify the resistance (R) gene(s) controlling the high level of resistance in this cultivar, we developed 286 F(9) recombinant inbred lines (RILs) from a cross between XZ3150 and the highly susceptible cultivar CO39. Inoculation of the RILs and an F(2) population from a cross between the two cultivars with the avirulent isolate 193-1-1 in the growth chamber indicated the presence of two dominant R genes in XZ3150. A linkage map with 134 polymorphic simple sequence repeat and single feature polymorphism markers was constructed with the genotype data of the 286 RILs. Composite interval mapping (CIM) using the results of 193-1-1 inoculation showed that two major R genes, designated Pi47 and Pi48, were located between RM206 and RM224 on chromosome 11, and between RM5364 and RM7102 on chromosome 12, respectively. Interestingly, the CIM analysis of the four resistant components of the RILs to the field blast population revealed that Pi47 and Pi48 were also the major genetic factors responsible for the field resistance in XZ3150. The DNA markers linked to the new R genes identified in this study should be useful for further fine mapping, gene cloning, and marker-aided breeding of blast-resistant rice cultivars.
authors.affiliation: Hunan Agricultural University, Changsha, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Guangping
authors.initials: G
authors.lastname: Feng
authors.forename: Suhua
authors.initials: S
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Jinling
authors.initials: J
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.forename: Weiting
authors.initials: W
authors.lastname: Yan
authors.forename: Lingchao
authors.initials: L
authors.lastname: Xu
authors.forename: Pingyong
authors.initials: P
authors.lastname: Sun
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.forename: Sujun
authors.initials: S
authors.lastname: Pan
authors.forename: Xionglun
authors.initials: X
authors.lastname: Liu
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Xiao
authors.forename: Erming
authors.initials: E
authors.lastname: Liu
authors.forename: Liangying
authors.initials: L
authors.lastname: Dai
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Wang
mesh_terms: D002681:China; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D005838:Genotype; D020082:Magnaporthe / Q000276:immunology / Q000472:pathogenicity*; D018598:Minisatellite Repeats / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics*; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D013045:Species Specificity
chemical_list: D005819:Genetic Markers