title: Quantification of quetiapine in human plasma by reverse phase high performance liquid chromatography.
abstract: The objective of the present investigation is to develop and validate a simple, economical and reliable high performance liquid chromatography method for the quantification of quetiapine (CAS 111974-72-2) in human plasma with a quantification limit sufficiently low to support pharmacokinetic studies. Imipramine hydrochloride (CAS 113-52-0) was used as internal standard. The validated method allows quantification of quetiapine in 15-1000 ng/mL. The method was shown to be precise (< 7% coefficient of variation, CV) and accurate (< or = 10% relative error, RE). The correlation coefficient for quetiapine was > 0.9970. The simplicity of the assay and rapid liquid-liquid extraction make it an attractive procedure in high-throughput bioanalysis of quetiapine.
authors.affiliation: Bioanalytical wing, Manipal AcuNova KH Clinical Research Center, Manipal, Karnataka, India.
authors.forename: Dev
authors.initials: D
authors.lastname: Prakash
authors.forename: Krishnamurthy
authors.initials: K
authors.lastname: Bhat
authors.forename: Raghavendra
authors.initials: R
authors.lastname: Shetty
authors.forename: Parveen
authors.initials: P
authors.lastname: Chaudhary
authors.forename: Sri Ram
authors.initials: SR
authors.lastname: Pathak
authors.forename: Amitabha
authors.initials: A
authors.lastname: Ghosh
mesh_terms: D000465:Algorithms; D014150:Antipsychotic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D003987:Dibenzothiazepines / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D000069348:Quetiapine Fumarate; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D014150:Antipsychotic Agents; D003987:Dibenzothiazepines; D007202:Indicators and Reagents; D012996:Solutions; D000069348:Quetiapine Fumarate