title: [The influence of acetylcholine on N receptor beta-subunit of the gastric epithelia and the gastric adenocarcinoma cells].
abstract: OBJECTIVE
  To study the influence of acetylcholine (ACh) on nicotinic receptor(N receptor) beta-subunit of the gastric epithelia and the gastric adenocarcinoma cells, and the difference of both cells.
  
  
  METHODS
  Immunohistochemistry method was used to examine the number, number density and surface density of N receptor beta-subunit in both cells cultured in vitro.
  
  
  RESULTS
  The number and number density of N receptor beta-subunit in the gastric adenocarcinoma cells were much more than that in the gastric epithelia (P < 0.05). But surface density of N receptor beta-subunit in the gastric adenocarcinoma cells were lower than that in the gastric epithelia (P < 0.05). ACh at 10(6) mol/L could increase the number, number density and surface density of N receptor beta-subunit in the gastric epithelia (P < 0.01). The increase effect could not be blocked by atropine. ACh had no effect on N receptor beta-subunit in the gastric adenocarcinoma cells.
  
  
  CONCLUSIONS
  ACh at low concentration initiates N receptor desensitization in the gastric epithelia. ACh has no effect on sensitivity of N receptor beta-subunit in the gastric adenocarcinoma cells.
authors.affiliation: Department of Physiology, Medical College of Qinghai University, Xining 810001, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Wang
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000230:Adenocarcinoma / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D005753:Gastric Mucosa / Q000166:cytology; D006801:Humans; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D011978:Receptors, Nicotinic; D000109:Acetylcholine