title: Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater.
abstract: A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater (MGW) and potato wastewater (PW) as culture medium. The maximum yield of 6.5 x 10(9) CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28 degrees C, pH 6.0-7.5 using the mixture of MGW and PW (1:9).
authors.affiliation: Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. gulikun-rcees@live.cn
authors.forename: Likun
authors.initials: L
authors.lastname: Gu
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Bai
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
authors.forename: Hongxun
authors.initials: H
authors.lastname: Zhang
mesh_terms: D020171:Botrytis / Q000254:growth & development; D002963:Cladosporium / Q000254:growth & development; D005670:Fusarium / Q000254:growth & development; D056507:Paenibacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010572:Pest Control, Biological / Q000379:methods*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012970:Sodium Glutamate / Q000378:metabolism*
chemical_list: D012336:RNA, Ribosomal, 16S; D012970:Sodium Glutamate