title: Kinetic models of guanidine hydrochloride-induced curing of the yeast [PSI+] prion.
abstract: A population of [PSI(+)] Saccharomyces cerevisiae cells can be cured of the [PSI(+)] prion by the addition of guanidine hydrochloride (GdnHCl). In this paper we extend existing nucleated polymerisation simulation models to investigate the mechanisms that might underlie curing. Our results are consistent with the belief that prions are dispersed through the cells at division following GdnHCl addition. A key feature of the simulation model is that the probability that a polymer is transmitted from mother to daughter during cell division is dependent upon the length of the polymer. The model is able to reproduce the essential features of data from several different experimental protocols involving addition and removal of GdnHCl.
authors.affiliation: School of Mathematics, Statistics and Actuarial Science, University of Kent, Canterbury, Kent CT2 7NF, United Kingdom.
authors.forename: K J
authors.initials: KJ
authors.lastname: Palmer
authors.forename: M S
authors.initials: MS
authors.lastname: Ridout
authors.forename: B J T
authors.initials: BJ
authors.lastname: Morgan
mesh_terms: D002455:Cell Division / Q000187:drug effects; D003198:Computer Simulation; D019791:Guanidine / Q000494:pharmacology*; D007700:Kinetics; D008954:Models, Biological; D010454:Peptide Termination Factors / Q000378:metabolism*; D011108:Polymers / Q000494:pharmacology; D011328:Prions / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010454:Peptide Termination Factors; D011108:Polymers; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D019791:Guanidine