title: Increased IL-17 expression is associated with pathology in a bovine model of tuberculosis.
abstract: The identification of bovine tuberculosis (bTB) biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and to improve the disease diagnosis and prognosis. The aim of this study was to understand the changing profile of the immune responses during the course of infection and to identify biomarkers associated with pathology. Here we describe the immune response developed in experimentally infected cattle with field Mycobacterium bovis strains. Blood samples were taken from each animal at different time points after M. bovis intratracheal infection and lymphocyte subset activation and cytokine mRNA expression were determined from peripheral blood mononuclear cells in response to purified protein derivative (PPDB). We found that CD4 and CD8 activation during the early stages of infection, together with IL-17 gene expression, were positively associated with pathology. The results of this study provide evidences of the role of IL-17 in the immunopathology of tuberculosis and support the use of IL-17 as a potential biomarker with predictive value of prognosis in bTB.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto y De Reseros, 1686 Hurlingham, Buenos Aires, Argentina.
authors.forename: Federico Carlos
authors.initials: FC
authors.lastname: Blanco
authors.forename: María Verónica
authors.initials: MV
authors.lastname: Bianco
authors.forename: Virginia
authors.initials: V
authors.lastname: Meikle
authors.forename: Sergio
authors.initials: S
authors.lastname: Garbaccio
authors.forename: Lucas
authors.initials: L
authors.lastname: Vagnoni
authors.forename: Marina
authors.initials: M
authors.lastname: Forrellad
authors.forename: Laura Inés
authors.initials: LI
authors.lastname: Klepp
authors.forename: Angel Adrián
authors.initials: AA
authors.lastname: Cataldi
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002417:Cattle; D015964:Gene Expression Regulation, Bacterial / Q000276:immunology*; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D008213:Lymphocyte Activation; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*; D011237:Predictive Value of Tests; D014373:Tuberculin / Q000276:immunology; D014380:Tuberculosis, Bovine / Q000235:genetics / Q000276:immunology*
chemical_list: D015415:Biomarkers; D020381:Interleukin-17; D014373:Tuberculin