title: Improved mobilization of the CD34(+) and CD133(+) bone marrow-derived circulating progenitor cells by freshly isolated intracoronary bone marrow cell transplantation in patients with ischemic heart disease.
abstract: Cell therapy is a promising novel option for treatment of cardiovascular disease. Because the role of bone marrow-derived circulating progenitor cells (BM-CPCs) after cell therapy is less clear, we analyzed in this randomized, controlled study the influence of intracoronary autologous freshly isolated bone marrow cell transplantation (BMC-Tx) by using a point-of-care system on cardiac function and on the mobilization of BM-CPCs in patients with ischemic heart disease (IHD). Fifty-six patients with IHD were randomized to either receive freshly isolated BMC-Tx or a control group that did not receive cell therapy. Peripheral blood concentrations of CD34/45(+) and CD133/45(+) CPCs were measured by flow cytometry pre-, immediately post-, and at 3, 6, and 12 months postprocedure in both groups. Global ejection fraction and the size of infarct area were determined by left ventriculography. We observed in patients with IHD after intracoronary transplantation of autologous freshly isolated BMCs-Tx at 3 and 12 months follow-up a significant reduction of the size of infarct area and increase of global ejection fraction as well as infarct wall movement velocity. The mobilization of CD34/45(+) and CD133/45(+) BM-CPCs significantly increased at 3, 6, and 12 months after cell therapy when compared with baseline in patients with IHD, although no significant changes were observed between pre- and immediately postintracoronary cell therapy administration. In the control group without cell therapy, there was no significant difference of CD34/45(+) and CD133/45(+) BM-CPCs mobilization between pre- and at 3, 6, and 12 months postcoronary angiography. Intracoronary transplantation of autologous freshly isolated BMCs by using a point-of-care system in patients with IHD may enhance and prolong the mobilization of CD34/45(+) and CD133/45(+) BM-CPCs in peripheral blood and this might increase the regenerative potency in IHD.
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Rostock-University, Rostock, Germany. dr_g_turan@hotmail.com
authors.forename: Ramazan Gökmen
authors.initials: RG
authors.lastname: Turan
authors.forename: Ilkay
authors.initials: I
authors.lastname: Bozdag-Turan
authors.forename: Jasmin
authors.initials: J
authors.lastname: Ortak
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Akin
authors.forename: Stephan
authors.initials: S
authors.lastname: Kische
authors.forename: Henrik
authors.initials: H
authors.lastname: Schneider
authors.forename: Cem Hakan
authors.initials: CH
authors.lastname: Turan
authors.forename: Tim Christopher
authors.initials: TC
authors.lastname: Rehders
authors.forename: Mathias
authors.initials: M
authors.lastname: Rauchhaus
authors.forename: Tilo
authors.initials: T
authors.lastname: Kleinfeldt
authors.forename: Ester
authors.initials: E
authors.lastname: Adolph
authors.forename: Micheal
authors.initials: M
authors.lastname: Brehm
authors.forename: Sedat
authors.initials: S
authors.lastname: Yokus
authors.forename: Stephan
authors.initials: S
authors.lastname: Steiner
authors.forename: Kurtulus
authors.initials: K
authors.lastname: Sahin
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Nienaber
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Ince
mesh_terms: D000071916:AC133 Antigen; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006321:Heart / Q000503:physiopathology; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010455:Peptides / Q000378:metabolism*; D019095:Point-of-Care Systems; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D013318:Stroke Volume; D014182:Transplantation, Autologous; D016277:Ventricular Function, Left
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D020097:Natriuretic Peptide, Brain