title: Decreased glucose tolerance and plasma adiponectin:resistin ratio in a mouse model of post-traumatic stress disorder.
abstract: OBJECTIVE
  Obesity and type 2 diabetes are among the most serious health pathologies worldwide. Stress has been proposed as a factor contributing to the development of these health risk factors; however, the underlying mechanisms that link stress to obesity and diabetes need to be further clarified. Here, we study in mice how chronic stress affects dietary consumption and how that relationship contributes to obesity and diabetes.
  
  
  METHODS
  C57BL/6J mice were subjected to chronic variable stress (CVS) for 15 days and subsequently fed with a standard chow or high-fat diet. Food intake, body weight, respiratory quotient, energy expenditure and spontaneous physical activity were measured with a customised calorimetric system and body composition was measured with nuclear magnetic resonance. A glucose tolerance test was also applied and blood glucose levels were measured with a glucometer. Plasma levels of adiponectin and resistin were measured using Lincoplex kits.
  
  
  RESULTS
  Mice under CVS and fed with a high-fat diet showed impaired glucose tolerance associated with low plasma adiponectin:resistin ratios.
  
  
  CONCLUSIONS
  This study demonstrates, in a novel mouse model, how post-traumatic stress disorder enhances vulnerability for impaired glucose metabolism in an energy-rich environment and proposes a potential adipokine-based mechanism.
authors.affiliation: Department of Medicinal and Biological Chemistry, College of Pharmacy and Center for Diabetes and Endocrine Research, College of Medicine, University of Toledo, Toledo, OH, USA.
authors.forename: T R
authors.initials: TR
authors.lastname: Castañeda
authors.forename: R
authors.initials: R
authors.lastname: Nogueiras
authors.forename: T D
authors.initials: TD
authors.lastname: Müller
authors.forename: R
authors.initials: R
authors.lastname: Krishna
authors.forename: E
authors.initials: E
authors.lastname: Grant
authors.forename: A
authors.initials: A
authors.lastname: Jones
authors.forename: N
authors.initials: N
authors.lastname: Ottaway
authors.forename: G
authors.initials: G
authors.lastname: Ananthakrishnan
authors.forename: P T
authors.initials: PT
authors.lastname: Pfluger
authors.forename: N
authors.initials: N
authors.lastname: Chaudhary
authors.forename: M B
authors.initials: MB
authors.lastname: Solomon
authors.forename: S C
authors.initials: SC
authors.lastname: Woods
authors.forename: J P
authors.initials: JP
authors.lastname: Herman
authors.forename: M H
authors.initials: MH
authors.lastname: Tschöp
mesh_terms: D052242:Adiponectin / Q000097:blood; D000818:Animals; D001823:Body Composition / Q000502:physiology; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000502:physiology; D005951:Glucose Tolerance Test; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D052243:Resistin / Q000097:blood; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
chemical_list: D052242:Adiponectin; D052243:Resistin