title: Spirafolide from bay leaf (Laurus nobilis) prevents dopamine-induced apoptosis by decreasing reactive oxygen species production in human neuroblastoma SH-SY5Y cells.
abstract: Reactive oxygen species (ROS) are important mediators in many neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. This study tested the neuroprotective effects of spirafolide, a compound purified from the leaves of Laurus nobilis L. (Lauraceae), against dopamine (DA)-induced apoptosis in human neuroblastoma SH-SY5Y cells. Following a 24-h exposure of cells to DA (final conc., 0.6 mM), we observed a marked increase in apoptosis, increased generation of ROS and decreased cell viability. Pretreatment of the cells for 24 h with spirafolide (0.4, 2, and 10 μM) before exposure to DA notably increased cell survival (p < 0.01) and lowered intracellular ROS levels (p < 0.01). These results indicate that spirafolide has neuroprotective effects against DA toxicity. These effects may contribute to the treatment of neurodegenerative diseases.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Korea.
authors.forename: Ahrom
authors.initials: A
authors.lastname: Ham
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.forename: Uk
authors.initials: U
authors.lastname: Koo
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Jongheon
authors.initials: J
authors.lastname: Shin
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D048588:Benzoxazines; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000494:pharmacology; D006801:Humans; D027442:Laurus; D019636:Neurodegenerative Diseases / Q000188:drug therapy; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012717:Sesquiterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D048588:Benzoxazines; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; C559585:spirafolide; D004298:Dopamine