title: [Nucleotide sequence analysis of A novel HLA-B*15:124 allele confirmed].
abstract: This study was purposed to investigate the nucleotide sequences of a novel HLA-B*15:124 allele and its molecular mechanism. The genomic DNA from whole blood was extracted by using commercial DNA extraction kit. The sequences of exon 2, 3 and 4 of HLA-B locus in the proband were amplified by PCR with group-specific primers, the PCR products were purified by enzymes digestion, then exon 2 to 4 of HLA-B locus for both orientations was sequenced. The results showed that 2 HLA-B alleles of proband were gained after amplification and sequencing of group-specific primers, among them one was a B*40:03, another was a novel allele. After BLAST analysis, the novel allele showed nucleotides different from HLA-B*15:52 in exon 3 at nucleotide position 427 A > T and 440 G > T which resulted in amino acid change from Thr to Ser at codon 143 and Trp to Leu at conon 147. It is concluded that a novel HLA-B allele has two different nucleotides. This HLA-B allele is identified and has been officially named B*15:124 by the WHO Nomenclature Committee.
authors.affiliation: Institute of Transfusion Medicine, Zhejiang Provincial Blood Center, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhe-Dong
authors.initials: ZD
authors.lastname: Han
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: He
authors.forename: Nan-Ying
authors.initials: NY
authors.lastname: Chen
authors.forename: Fa-Ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Hang-Jun
authors.initials: HJ
authors.lastname: Lü
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D000483:Alleles; D001483:Base Sequence; D005091:Exons; D005260:Female; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens