title: [Effects of gathering season and three age affect on main active components of Taxus madia].
abstract: OBJECTIVE
  To reveal the influence of harvest season and tree age on the content of taxol and 10-DAB III, and provide the basis for the harvest time of Taxus madia.
  
  
  METHODS
  Branches and leaves of the labeled 25 three-year-old plants and 25 five-year-old plants were collected every two months from March 26,2009 to January 26, 2010. Taxol and 10-DAB III content of different age and growth season of Taxus in branches were determined by HPLC.
  
  
  RESULTS
  Taxol and 10-DAB III content were significantly different in different harvesting age. The content of five-year-old plants was significantly higher than that of three-year-old plants. Taxol and 10-DAB III contents were significantly different in different harvesting season, and the highest content of taxol and 10-DAB III was 0.56, 0.32 mg x g(-1), respectively, in May.
  
  
  CONCLUSIONS
  The May is the suitable harvest season for T. madia, but the suitable harvest age need further study which according to the main active component and biomass accumulation.
authors.affiliation: Research and Development Center for Natural Medicine, A Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Shi
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Xu
authors.forename: Shunhua
authors.initials: S
authors.lastname: Fu
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: He
authors.forename: Hongliang
authors.initials: H
authors.lastname: Du
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D017239:Paclitaxel / Q000032:analysis / Q000494:pharmacology*; D028321:Plant Preparations / Q000494:pharmacology; D012621:Seasons; D043823:Taxoids / Q000032:analysis / Q000494:pharmacology; D020946:Taxus / Q000737:chemistry / Q000187:drug effects*; D014197:Trees / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D028321:Plant Preparations; D043823:Taxoids; D017239:Paclitaxel