title: A study in three European IBD cohorts confirms that the ATG16L1 c.898A>G (p.Thr300Ala) variant is a susceptibility factor for Crohn's disease.
abstract: OBJECTIVE
  A recent study reported that a nonsynonymous SNP rs2241880 (c.898A>G, p.Thr300Ala) within ATG16L1 confers susceptibility to Crohn's disease (CD). We analyzed ATG16L1 c.898A>G in three independent European inflammatory bowel disease (IBD) cohorts from Germany, Hungary and the Netherlands.
  
  
  METHODS
  In total, we included 910 European IBD patients and compared the ATG16L1 c.898A>G genotype frequency with 707 ethnically matched healthy controls. We included patients from 3 populations originating from Germany (CD n=310; ulcerative colitis [UC] n=179), Hungary (CD n=147; UC n=117), and the Netherlands (CD n=157). Subtyping analysis was performed in respect to CARD15 alterations and clinical characteristics.
  
  
  RESULTS
  We found a highly significant association of c.898A>G to CD. The association was significant (p=0.0005) for the total CD cohort but also for the individual populations from Germany (p=0.02) and Netherlands (p=0.02) whereas in the Hungarian CD patients a clear trend was observed (p=0.19; OR 1.227, 95% CI 0.910; 1.654). No association was found between c.898A>G and UC. No statistical interactions were observed between ATG16L1 c.898A>G and CARD15 variants. Furthermore no association to a CD subphenotype was detected.
  
  
  CONCLUSIONS
  We confirm that ATG16L1 variant c898A>G confers a risk variant for CD but is not associated with a distinct CD phenotype.
authors.affiliation: Department of Gastroenterology, Hepatology and Endocrinology, Charité, Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Büning
authors.forename: Tahir
authors.initials: T
authors.lastname: Durmus
authors.forename: Tamas
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authors.lastname: Molnar
authors.forename: Dirk J
authors.initials: DJ
authors.lastname: de Jong
authors.forename: Joost P H
authors.initials: JP
authors.lastname: Drenth
authors.forename: Thomas
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authors.lastname: Fiedler
authors.forename: Enno
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authors.lastname: Gentz
authors.forename: Theodor
authors.initials: T
authors.lastname: Todorov
authors.forename: Verena
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authors.lastname: Haas
authors.forename: Sabine
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authors.lastname: Buhner
authors.forename: Andreas
authors.initials: A
authors.lastname: Sturm
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Baumgart
authors.forename: Ferenc
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authors.lastname: Nagy
authors.forename: Janos
authors.initials: J
authors.lastname: Lonovics
authors.forename: Olfert
authors.initials: O
authors.lastname: Landt
authors.forename: Andreas
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authors.lastname: Kage
authors.forename: Herbert
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authors.lastname: Büning
authors.forename: Renate
authors.initials: R
authors.lastname: Nickel
authors.forename: Janine
authors.initials: J
authors.lastname: Büttner
authors.forename: Herbert
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authors.lastname: Lochs
authors.forename: Hartmut H-J
authors.initials: HH
authors.lastname: Schmidt
authors.forename: Heiko
authors.initials: H
authors.lastname: Witt