title: Biologic characteristics of bone marrow-derived mesenchymal stem cells from a patient with thalassemia syndrome.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiating morphologically and functionally into several mesenchymal tissues. There have been contrasting data on whether MSCs are altered in various hematologic disorders.
  
  
  METHODS
  We isolated bone marrow (BM)-derived MSCs from a patient with thalassemia syndrome to compare phenotypic and functional characteristics to those from normal healthy donor.
  
  
  RESULTS
  No differences were observed between MSCs from thalassemia syndrome (T-MSCs) and those from normal healthy donor in terms of morphology, phenotype, karyotype, multidifferentiation capacity. In mixed lymphocyte reaction, T-MSCs strongly inhibited the proliferation of allogeneic T cells in association with reduced proportion of CD3(+), CD4(+), and CD8(+) cells. Furthermore, the fraction of Treg cells was increased under the culture with T-MSCs, suggesting that T-MSCs exert normal immunomodulatory function. In addition, T-MSCs expressed hematopoietic cytokines and supported hematopoiesis, which was comparable to those from normal BM-derived MSCs.
  
  
  CONCLUSIONS
  T-MSCs exhibited normal phenotype, karyotype as well as normal immunomodulatory function, and autologous MSCs from patients with thalassemia syndrome may be an attractive source of stem cell in terms of hematopoietic support as well as immunomodulatory activity.
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Yoon
authors.forename: E S
authors.initials: ES
authors.lastname: Kim
authors.forename: D W
authors.initials: DW
authors.lastname: Hwang
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: B K
authors.initials: BK
authors.lastname: Kim
authors.forename: B B
authors.initials: BB
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Choi
authors.forename: Y Y
authors.initials: YY
authors.lastname: Lee
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics; D006801:Humans; D056747:Immunomodulation / Q000276:immunology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D013577:Syndrome; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013789:Thalassemia / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
chemical_list: D016207:Cytokines