title: Physiological characterization of the Arabidopsis thaliana oxidation-related zinc finger 1, a plasma membrane protein involved in oxidative stress.
abstract: The CCCH-type zinc finger proteins are a superfamily containing tandem zinc-binding motifs involved in many aspects of plant growth and development. However, the precise role of these proteins involved in plant stress tolerance is poorly understood. This study was to examine the regulatory and functional role of the CCCH-type zinc finger protein, AtOZF1 (At2g19810), under oxidative stress. Interestingly, the AtOZF1 protein was localized in the plasma membrane. The AtOZF1 transcripts were highly induced by treatment with hydrogen peroxide, abscisic acid and salinity. The AtOZF1-overexpressing plants were relatively resistant to oxidative stress than wild-type and T-DNA insertion mutant atozf1. Malondialdehyde, a decomposition product of lipid peroxidation, accumulated in atozf1 mutants more than in wild-type and AtOZF1-overexpressing plants. Furthermore, atozf1 mutants displayed lower activities of catalase and guaiacol peroxidase, higher chlorosis, and down-regulated expression of antioxidant genes under oxidative stress. Taken together, these observations demonstrate that AtOZF1 is required for the tolerance of Arabidopsis to oxidative stress.
authors.affiliation: Department of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Chung
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Ju
authors.forename: Hyun-Seok
authors.initials: HS
authors.lastname: Na
authors.forename: Dong Ju
authors.initials: DJ
authors.lastname: Lee
authors.forename: Hyeon-Sook
authors.initials: HS
authors.lastname: Cheong
authors.forename: Cheol Soo
authors.initials: CS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018384:Oxidative Stress
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C570339:OZF1 protein, Arabidopsis; D006861:Hydrogen Peroxide