title: [Changes of expression and distribution of prohibitin in oxidative stressed cardiomyocyte and its biological significance].
abstract: OBJECTIVE
  To approach the expression of prohibitin in oxidative stressed cardiomyocytes.
  
  
  METHODS
  The oxidative stress model was established by treating neonatal cardiomyocytes with H2O2. Injury of cardiomyocytes were evaluated by detecting the LDH activity and MTT cell survival rate.The expression level of prohibitin was examined via the Western-blotting. The ability of mitochondrial oxidative phosphorylation was determined by measuring ATP synthesis via H+ -ATPase. Mitochondrial membrane potential was detected by flow cytometry using Rhl23.
  
  
  RESULTS
  LDH activity increased significantly after exposure to H202, while the cell survival rate decreased by 34.51%-65.5%. The contents of mitochondrial prohibitin in stress group was much higher than that in control group. At the same time, the ability of ATP synthesis decreased by 60% and mitochondrial transmembrane potential decreased too.
  
  
  CONCLUSIONS
  Express of prohibitin in oxidative stress cardiomyocytes was compensated increase. Prohibitin translocated to mitochondria after oxidative stress. Oxidative stress led to mitochondrial dysfunction.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ren
authors.forename: Ling-jia
authors.initials: LJ
authors.lastname: Qian
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D000091402:Prohibitins; D012097:Repressor Proteins; D007770:L-Lactate Dehydrogenase