title: Proteases and phosphatases during Leishmania-macrophage interaction: paving the road for pathogenesis.
abstract: The outcome of Leishmania infection depends both on host and pathogen factors. Macrophages, the specialized host cells for uptake and intracellular development of Leishmania, play a central role in the control of infection. Leishmania has evolved strategies to downregulate host cell functions, largely mediated by the parasite-induced activation of macrophage protein tyrosine phosphatases (PTPs). We have recently identified PTP1B and TCPTP as two additional PTPs engaged upon Leishmania infection and have unraveled an intimate interaction between the Leishmania surface protease GP63 and host PTPs, which mediates a mechanism of cleavage-dependent PTP activation. Here we discuss new perspectives for GP63-mediated parasite virulence and propose putative mechanisms of GP63 internalization into host macrophages and access to intracellular substrates.
authors.affiliation: Centro Internacional de Entrenamiento e Investigaciones Médicas, CIDEIM, Cali, Colombia.
authors.forename: María Adelaida
authors.initials: MA
authors.lastname: Gómez
authors.forename: Martin
authors.initials: M
authors.lastname: Olivier
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D007891:Leishmania / Q000201:enzymology / Q000472:pathogenicity / Q000502:physiology*; D008264:Macrophages / Q000276:immunology / Q000469:parasitology*; D008666:Metalloendopeptidases / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014774:Virulence
chemical_list: D015800:Protozoan Proteins; D017027:Protein Tyrosine Phosphatases; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania