title: A clinically relevant, syngeneic model of spontaneous, highly metastatic B16 mouse melanoma.
abstract: We report a syngeneic model of spontaneous metastatic B16-F10 mouse melanoma in C57/BL6 mice with a very high metastatic frequency that mimics clinical metastatic melanoma. The B16 melanoma cells were injected between the skin and cartilage on the dorsal side of the ear. The model generated lymphatic and visceral metastases in all of the tested animals. In mice with large primary tumors, tumor weight correlated with the tumor growth time and also with the number of metastases in lymph nodes and organs. The dorsal ear space between the skin and cartilage enables both lymphatic and hematogenous metastatic spread. The model should be useful to study the mechanism of melanoma metastasis and to develop therapy for this currently untreatable disease.
authors.affiliation: Department of Tumor Biology, Third Faculty of Medicine Charles University, Ruska 87, 100 34 Prague, Czech Republic. vbobek@centrum.cz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Bobek
authors.forename: Katarina
authors.initials: K
authors.lastname: Kolostova
authors.forename: Daniela
authors.initials: D
authors.lastname: Pinterova
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kacprzak
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Adamiak
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kolodziej
authors.forename: Michael
authors.initials: M
authors.lastname: Boubelik
authors.forename: Martina
authors.initials: M
authors.lastname: Kubecova
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D008207:Lymphatic Metastasis; D008546:Melanoma, Experimental / Q000473:pathology* / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation