title: Structural basis of the association of HIV-1 matrix protein with DNA.
abstract: HIV-1 matrix (MA) is a multifunctional protein that is synthesized as a polyprotein that is cleaved by protease during viral maturation. MA contains a cluster of basic residues whose role is controversial. Proposed functions include membrane anchoring, facilitating viral assembly, and directing nuclear import of the viral DNA. Since MA has been reported to be a component of the preintegration complex (PIC), we have used NMR to probe its interaction with other PIC components. We show that MA interacts with DNA and this is likely sufficient to account for its association with the PIC.
authors.affiliation: Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Mengli
authors.initials: M
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Robert
authors.initials: R
authors.lastname: Craigie
authors.forename: G Marius
authors.initials: GM
authors.lastname: Clore
mesh_terms: D001483:Base Sequence; D002151:Calorimetry / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics*; D054298:Human Immunodeficiency Virus Proteins / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010641:Phenotype; D020815:Polyproteins / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012313:RNA / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D054298:Human Immunodeficiency Virus Proteins; D009841:Oligonucleotides; D020815:Polyproteins; D012313:RNA; D004247:DNA