title: Mucin from rheumatoid arthritis synovial fluid enhances interleukin-6 production by human peripheral blood mononuclear cells.
abstract: The carbohydrate chains represented by mucins (MUCs) are expressed by a variety of normal and malignant secretory epithelial cells and induce a variety of immunoreactions. To find new mucins related to the pathogenesis of rheumatoid arthritis (RA), we examined high-molecular-weight molecules inducing cytokines on human peripheral blood mononuclear cells (PBMCs) in synovial fluid from affected joints. We found a high-molecular-weight substance that induces interleukin 6 production on PBMCs in RA synovial fluid on gel filtration. MUC-1 was present in the resulting fractions, although they had been purified by CsCl density gradient centrifugation. We also found that MUC-1 was expressed on synovial cells and infiltrating inflammatory mononuclear cells on the sublining layer and lymphoid follicles in RA synovial tissues. CD68-positive superficial synovial cells colocalized with MUC-1 and CD68-positive macrophages were in contact with MUC-1-positive mononuclear cells. These findings imply that mucins, including MUC-1, may be related to immunoinflammatory reactions in the pathogenesis of RA.
authors.affiliation: Department of Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Hamaguchi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawahito
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Ishino
authors.forename: Noriko
authors.initials: N
authors.lastname: Takeuchi
authors.forename: Daisaku
authors.initials: D
authors.lastname: Tokunaga
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hojo
authors.forename: Aihiro
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kadoya
authors.forename: Takahiro
authors.initials: T
authors.lastname: Seno
authors.forename: Masataka
authors.initials: M
authors.lastname: Kohno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakada
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000473:pathology; D015153:Blotting, Western; D002465:Cell Movement; D002499:Centrifugation, Density Gradient; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D015850:Interleukin-6 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D018396:Mucin-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013582:Synovial Fluid / Q000737:chemistry* / Q000166:cytology; D013583:Synovial Membrane / Q000276:immunology / Q000473:pathology; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015850:Interleukin-6; D018396:Mucin-1; C067980:CD68 antigen, human; D000097382:CD68 Molecule