title: Amylase and lipase detection in hemorrhaged animals treated with HBOC-201.
abstract: HBOC-201 may alter lipase and amylase detection on chemistry analyzers using optical methods and affect pancreatic function after trauma. Amylase and lipase measurements were correlated against HBOC-201 to evaluate interference on samples spiked with 0-6g/dL HBOC-201. The detection threshold was 2.5g/dL or none when measured, respectively, on Vitros 250 or Advia 1650 instruments. Amylase and lipase from blood samples collected from 55% EBV hemorrhaged Yucatan min-pigs showed peaks around 24-48 hours. Amylase increase was not significant between treatments but lipase was higher in HBOC-201-treated animals. Animals particularly affected by the injury had elevated enzymes after hemorrhagic shock, without significant clinical consequences.
authors.affiliation: Naval Medical Research Center, Silver Spring, MD 20910-7500, USA. francoise.arnaud@MED.NAVY.MIL
authors.forename: Françoise
authors.initials: F
authors.lastname: Arnaud
authors.forename: Richard
authors.initials: R
authors.lastname: McCarron
authors.forename: Daniel
authors.initials: D
authors.lastname: Freilich
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D001802:Blood Substitutes / Q000008:administration & dosage*; D006454:Hemoglobins / Q000008:administration & dosage*; D008049:Lipase / Q000097:blood; D055620:Optical Phenomena; D010179:Pancreas / Q000293:injuries* / Q000503:physiopathology; D012016:Reference Values; D012771:Shock, Hemorrhagic / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy / Q000201:enzymology / Q000209:etiology; D013552:Swine; D013556:Swine, Miniature; D014947:Wounds and Injuries / Q000150:complications / Q000175:diagnosis* / Q000188:drug therapy
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C096798:HBOC 201; D008049:Lipase; D000681:Amylases