title: Hormonal effects on Tetrahymena: change in case of combined treatment.
abstract: In order to approach their natural conditions, populations of Tetrahymena were kept in Losina-Losinky's salt solution for 1 h, than in the tryptone+yeast medium. During this time they were treated with histamine, serotonin or insulin, or with the combinations of these hormones. Effect of the combined treatments on the production of serotonin (5HT), or adrenocorticotropic hormone (ACTH) or triiodothyronine (T₃) by the cells was compared to the effect of single-hormone treatments. Significant differences were seen between the results obtained following the single or combined treatments. There was no summation of the effects, however an elevation or diminution of the hormone production was observed after the combined treatment, as compared with the untreated controls or with the use of one of the hormones in the samples. The experiments demonstrate that there is a hormonal regulation between the Tetrahymena cells and the hormones influence each other's effect.
authors.affiliation: Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary. csagyor@dgci.sote.hu
authors.forename: G
authors.initials: G
authors.lastname: Csaba
authors.forename: Eszter
authors.initials: E
authors.lastname: Lajkó
authors.forename: Eva
authors.initials: E
authors.lastname: Pállinger
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006632:Histamine / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D010461:Peptones; D012701:Serotonin / Q000096:biosynthesis / Q000494:pharmacology*; D012965:Sodium Chloride; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000378:metabolism*; D014284:Triiodothyronine / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D007328:Insulin; D010461:Peptones; D014284:Triiodothyronine; D012701:Serotonin; D012965:Sodium Chloride; C001830:tryptones; D006632:Histamine; D000324:Adrenocorticotropic Hormone