title: Human uremic plasma and not urea induces exuberant secretion of leptin in 3T3-L1 adipocytes.
abstract: Chronic kidney disease (CKD) is frequently associated with malnutrition, anorexia, and hyperleptinemia. This study was designed to test the hypothesis that a component of the uremic milieu may trigger leptin release by adipocytes. To this end, mouse 3T3-L1 adipocytes were incubated for 16 hours in culture medium containing urea (80 mM) or plasma from either healthy volunteers or patients with CKD (20%, v/v). Uremic plasma and not urea induced a large release of leptin (+557%, P < .01). These results suggest that the hyperleptinemia reported in patients with CKD, could be, at least in part, because of an overproduction of leptin by the adipose tissue.
authors.affiliation: Université de Lyon, Lyon, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Kalbacher
authors.forename: Laetitia
authors.initials: L
authors.lastname: Koppe
authors.forename: Bader
authors.initials: B
authors.lastname: Zarrouki
authors.forename: Nicolas J
authors.initials: NJ
authors.lastname: Pillon
authors.forename: Denis
authors.initials: D
authors.lastname: Fouque
authors.forename: Christophe O
authors.initials: CO
authors.lastname: Soulage
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000652:urine*; D020738:Leptin / Q000378:metabolism*; D008297:Male; D051379:Mice; D008875:Middle Aged; D014508:Urea / Q000378:metabolism*; D014511:Uremia / Q000097:blood*
chemical_list: D020738:Leptin; D014508:Urea