title: Huntingtin-interacting protein 1-related is required for accurate congression and segregation of chromosomes.
abstract: Huntingtin-interacting protein 1-related (HIP1r) is known to function in clathrin-mediated endocytosis and regulation of the actin cytoskeleton, which occurs continuously in non-dividing cells. This study reports a new function for HIP1r in mitosis. Green fluorescent protein-fused HIP1r localizes to the mitotic spindles. Depletion of HIP1r by RNA interference induces misalignment of chromosomes and prolonged mitosis, which is associated with decreased proliferation of HIP1r-deficeint cells. Chromosome misalignment leads to missegregation and ultimately production of multinucleated cells. Depletion of HIP1r causes persistent activation of the spindle checkpoint in misaligned chromosomes. These findings suggest that HIP1r plays an important role in regulating the attachment of spindle microtubules to chromosomes during mitosis, an event that is required for accurate congression and segregation of chromosomes. This finding may provide new insights that improve the understanding of various human diseases involving HIP1r as well as its fusion genes.
authors.affiliation: Department of Chemistry, Pukyong National University, Busan 608-737, Korea. parksj@pknu.ac.kr
authors.forename: Sun Joo
authors.initials: SJ
authors.lastname: Park
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D020090:Chromosome Segregation; D002875:Chromosomes / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008840:Microfilament Proteins; D008938:Mitosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C489537:HIP1R protein, human; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins