title: Biomarker discovery and identification from non-small cell lung cancer sera.
abstract: Currently, serum biomarkers might usually be thought not to be used for early detection of lung cancer by some researchers. In this study, we used a highly optimized ClinProt-matrix-assisted laser desorption/ionization time-of flight mass spectrometer (MALDI-TOF-MS) to screen non-small cell lung carcinoma (NSCLC) markers in serum. A training set of spectra derived from 45 NSCLC patients, 24 patients with benign lung diseases (BLDs) and 21 healthy individuals, was used to develop a proteomic pattern that discriminated cancer from non-cancer effectively. A test set, including 74 cases (29 NSCLC patients and 45 controls), was used to validate this pattern. After cross-validation, the classifier showed sensitivity and specificity, 86.20% and 80.00%, respectively. Remarkably, 100% of early stage serum samples could be correctly classified as lung cancer. Furthermore, the differential peptides of 1865Da and 4209Da were identified as element of component 3 and eukaryotic peptide chain release factor GTP-binding subunit ERF, respectively. The patterns we described and peptides we identified may have clinical utility as surrogate markers for detection and classification of NSCLC.
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.forename: Shuan-ying
authors.initials: SY
authors.lastname: Yang
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Lin
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Shang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Shu-fen
authors.initials: SF
authors.lastname: Huo
authors.forename: Li-na
authors.initials: LN
authors.lastname: Bu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Yan-dong
authors.initials: YD
authors.lastname: Nan
authors.forename: Hua-dong
authors.initials: HD
authors.lastname: Zheng
authors.forename: Yan-feng
authors.initials: YF
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D010455:Peptides / Q000097:blood*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D010455:Peptides