title: A genetic comparison of Brucella abortus isolates from animals and humans by using an MLVA assay.
abstract: The MLVA assay is known to have a high ability to identify and discriminate Brucella species, so that it can be used as an epidemiological tool to discriminate Brucella isolates originating from restricted geographic sources. In this study, the genetic profiles of 38 B. abortus isolates from humans were analyzed and compared with genotypes from animal isolates in South Korea. As a result, it was found that they did not show high genetic diversity and were compacted. They were clustered together with animal isolates, showing a significant correlation to regional distributions. With its ability to prove a significant genetic correlation among B. abortus isolates from animals and humans in South Korea, the MLVA assay could be utilized as part of a program to control and eradicate brucellosis, one of the major zoonoses. This study represents the first data of genetic correlation of B. abortus isolates from humans and animals in South Korea.
authors.affiliation: National Veterinary Research and Quarantine Service (NVRQS), Anyang 430-824, Korea. herm@nvrqs.go.kr
authors.forename: Moon
authors.initials: M
authors.lastname: Her
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Kang
authors.forename: Jong-Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: In-Yeong
authors.initials: IY
authors.lastname: Hwang
authors.forename: Suk-Chan
authors.initials: SC
authors.lastname: Jung
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Mi Yeoun
authors.initials: MY
authors.lastname: Park
authors.forename: Hansang
authors.initials: H
authors.lastname: Yoo
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002003:Brucella abortus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002006:Brucellosis / Q000382:microbiology*; D002007:Brucellosis, Bovine / Q000382:microbiology*; D002417:Cattle; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D058889:Molecular Typing; D056910:Republic of Korea; D015047:Zoonoses / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial