title: Analysis of 5' nontranslated region of hepatitis A viral RNA genotype I from South Korea: comparison with disease severities.
abstract: The aim of the study was to analyze genotype I hepatitis A virus (HAV) 5' nontranslated region (NTR) sequences from a recent outbreak in South Korea and compare them with reported sequences from Japan. We collected a total of 54 acute hepatitis A patients' sera from HAV genotype I [27 severe disease (prothrombin time INR ≥ 1.50) and 27 mild hepatitis (prothrombin time INR <1.00)], performed nested RT-PCR of 5' NTR of HAV directly sequenced from PCR products (∼ 300 bp), and compared them with each other. We could detect HAV 5'NTR sequences in 19 of the 54 (35.1%) cases [12 of 27 severe cases (44.4%) and 7 of 27 self-limited cases (25.9%)], all of which were subgenotype IA. Sequence analysis revealed that sequences of severe disease had 93.6%-99.0% homology and of self-limited disease 94.3%-98.6% homology, compared to subgenotype IA HAV GBM wild-type IA sequence. In this study, confirmation of the 5'NTR sequence differences between severe disease and mild disease was not carried out. Comparison with Japanese HAV A10 revealed (222)C to G or T substitution in 8/12 cases of severe disease and (222)C to G or T and (392)G to A substitutions in 5/7 and 4/7 cases of mild disease, respectively, although the nucleotide sequences in this study showed high homology (93.6%-100%). In conclusion, HAV 5'NTR subgenotype IA from Korea had relatively high homology to Japanese sequences previously reported from Japan, and this region would be considered one of the antiviral targets. Further studies will be needed.
authors.affiliation: Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan. kandat-cib@umin.ac.jp
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kanda
authors.forename: Sook-Hyang
authors.initials: SH
authors.lastname: Jeong
authors.forename: Fumio
authors.initials: F
authors.lastname: Imazeki
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Osamu
authors.initials: O
authors.lastname: Yokosuka
mesh_terms: D020121:5' Untranslated Regions; D017931:DNA Primers / Q000235:genetics; D005814:Genes, Viral; D005838:Genotype; D030041:Hepatitis A virus / Q000235:genetics*; D006801:Humans; D019934:International Normalized Ratio; D007564:Japan; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000235:genetics*; D056910:Republic of Korea; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D020121:5' Untranslated Regions; D017931:DNA Primers; D009711:Nucleotides; D012367:RNA, Viral