title: Fabrication of chitin-chitosan/nano TiO2-composite scaffolds for tissue engineering applications.
abstract: In this study, we prepared chitin-chitosan/nano TiO(2) composite scaffolds using lyophilization technique for bone tissue engineering. The prepared composite scaffold was characterized using SEM, XRD, FTIR and TGA. In addition, swelling, degradation and biomineralization capability of the composite scaffolds were evaluated. The developed composite scaffold showed controlled swelling and degradation when compared to the control scaffold. Cytocompatibility of the scaffold was assessed by MTT assay and cell attachment studies using osteoblast-like cells (MG-63), fibroblast cells (L929) and human mesenchymal stem cells (hMSCs). Results indicated no sign of toxicity and cells were found attached to the pore walls within the scaffolds. These results suggested that the developed composite scaffold possess the prerequisites for tissue engineering scaffolds and it can be used for tissue engineering applications.
authors.affiliation: Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham Elamakkara, Kochi 682026, Kerala, India. rjayakumar@aims.amrita.edu
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
authors.forename: Roshni
authors.initials: R
authors.lastname: Ramachandran
authors.forename: V V
authors.initials: VV
authors.lastname: Divyarani
authors.forename: K P
authors.initials: KP
authors.lastname: Chennazhi
authors.forename: H
authors.initials: H
authors.lastname: Tamura
authors.forename: S V
authors.initials: SV
authors.lastname: Nair
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002686:Chitin / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008903:Minerals / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D008903:Minerals; D002686:Chitin; C009495:titanium dioxide; D048271:Chitosan; D014025:Titanium