title: Three-step electrodeposition synthesis of self-doped polyaniline nanofiber-supported flower-like Au microspheres for high-performance biosensing of DNA hybridization recognition.
abstract: A three-step electrodeposition method has been successfully adopted to fabricate morphology-controlled novel Au microspheres on self-doped polyaniline nanofibers (nanoSPAN) modified glassy carbon electrode. The deposition conditions, such as HAuCl(4) concentration and deposition step, have significant influences on the morphologies and electrochemical properties of the resulted Au microspheres. Well hierarchical and homogeneously dispersed flower-like Au microspheres (HHFAu) were obtained under optimal conditions by the three-step electrodeposition strategy in 5.0mM HAuCl(4) solution. HHFAu possess large surface area, excellent electron transfer ability and good biocompatibility. The DNA probe could be effectively attached to HHFAu and thus a high-performance DNA biosensor was constructed by using electrochemical impedance spectroscopy as detection method. A gene fragment of the cauliflower mosaic virus 35S gene, which is related to one of the screening genes for the transgenically modified plants, has been satisfactorily detected. The linear range was from 1.0 × 10(-13)M to 1.0 × 10(-6)M and the detection limit was 1.9 × 10(-14)M. This HHFAu/nanoSPAN-based impedance biosensing platform holds great promise for the detection of other biological and chemical molecules.
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Kui
authors.initials: K
authors.lastname: Jiao
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D006046:Gold; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization
chemical_list: D000814:Aniline Compounds; C416807:polyaniline; D006046:Gold; D004247:DNA