title: Derivatization strategies for the determination of biogenic amines in wines by chromatographic and electrophoretic techniques.
abstract: This paper revises the derivatization approaches for the determination of biogenic amines in wines. Since most of these amines display poor spectroscopic features to be detected by UV absorption or emission (fluorescence) spectroscopy, derivatization is necessary to attain the desired sensitivity. Reagents such as o-phthaldialdehyde, fluorenylmethylchloroformate, dansyl-Cl and dabsyl-Cl have widely been used for analytical labeling through amino group. A comparison of features of off- and on-line pre- and post chromatographic/electrophoretic labeling is given using 1,2-naphthoquinone-4-sulfonate (NQS) as an example. The evaluation of the influence of the wine sample composition on the derivatization process indicates that pre-column labeling may undergo more severe matrix effects.
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028 Barcelona, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Hernández-Cassou
authors.forename: Javier
authors.initials: J
authors.lastname: Saurina
mesh_terms: D001679:Biogenic Amines / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009285:Naphthoquinones / Q000737:chemistry; D014920:Wine / Q000032:analysis*
chemical_list: D001679:Biogenic Amines; D009285:Naphthoquinones; C011942:1,2-naphthoquinone-4-sulfonate