title: Western blotting: sample preparation to detection.
abstract: Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.
authors.affiliation: Research and Development, EMD Chemicals Inc.
authors.forename: Anna
authors.initials: A
authors.lastname: Eslami
authors.forename: Jesse
authors.initials: J
authors.lastname: Lujan
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins / Q000032:analysis*; D005465:Fluorocarbon Polymers
chemical_list: D003101:Collodion; D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers