title: Function of a retrotransposon nucleocapsid protein.
abstract: Long terminal repeat (LTR) retrotransposons are not only the ancient predecessors of retroviruses, but they constitute significant fractions of the genomes of many eukaryotic species. Studies of their structure and function are motivated by opportunities to gain insight into common functions of retroviruses and retrotransposons, diverse mechanisms of intracellular genomic mobility, and host factors that diminish or enhance retrotransposition. This review focuses on the nucleocapsid (NC) protein of a Saccharomyces cerevisiae LTR retrotransposon, the metavirus, Ty3. Retrovirus NC promotes genomic (g)RNA dimerization and packaging, tRNA primer annealing, reverse transcription strand transfers, and host protein interactions with gRNA. Studies of Ty3 NC have revealed key roles for Ty3 NC in formation of retroelement assembly sites (retrosomes), and in chaperoning primer tRNA to both dimerize and circularize Ty3 gRNA. We speculate that Ty3 NC, together with P-body and stress-granule proteins, plays a role in transitioning Ty3 RNA from translation template to gRNA, and that interactions between the acidic spacer domain of Ty3 Gag3 and the adjacent basic NC domain control condensation of the virus-like particle.
authors.affiliation: Department of Biological Chemistry, School of Medicine, University of California, Irvine, CA USA. sbsandme@uci.edu
authors.forename: Suzanne B
authors.initials: SB
authors.lastname: Sandmeyer
authors.forename: Kristina A
authors.initials: KA
authors.lastname: Clemens
mesh_terms: D000818:Animals; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D018626:Retroelements / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics / Q000378:metabolism; D048348:Reverse Transcription / Q000502:physiology; D020079:Terminal Repeat Sequences / Q000235:genetics; D019065:Virus Assembly / Q000502:physiology
chemical_list: D019590:Nucleocapsid Proteins; D018626:Retroelements