title: Graphene oxide sheet-mediated silver enhancement for application to electrochemical biosensors.
abstract: Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 × 10(2) to 1.8 × 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfate-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wu
authors.forename: Dun
authors.initials: D
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry / Q000648:ultrastructure; D006108:Graphite / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000737:chemistry*; D012834:Silver / Q000032:analysis*
chemical_list: D010087:Oxides; D012834:Silver; D006108:Graphite