title: Adhesion phenotypes of pigs of Chinese and Western breeds and a White Duroc-Erhualian crossbreed with regard to susceptibility to enterotoxigenic Escherichia coli with fimbrial adhesins K99, 987P, and F41.
abstract: OBJECTIVE
  To investigate adhesion phenotypes of pigs of Chinese and Western breeds and a specific crossbreed with regard to enterotoxigenic Escherichia coli (ETEC) with fimbrial adhesins K99 (F5), 987P (F6), and F41 (F7).
  
  
  METHODS
  Purebred 6- to 8-week-old pigs of 3 Western breeds introduced into China (n = 144) and 12 Chinese breeds (148) and 1,330 adult White Duroc-Erhualian crossbred pigs.
  
  
  METHODS
  Brush border preparations were prepared from jejunal specimens collected from each pig following euthanasia. Each preparation was incubated with ETEC strains that had fimbrial adhesins K99, 987R or F41; an ETEC K88(-) strain was used as a negative control sample. The mean number of brush border-bound bacteria in aliquots of the bacteria-brush border suspensions (determined via phase-contrast microscopy) was used to determine each pig's adhesion phenotype for ETEC K99, 987R and F41 strains; the phenotype was classified as adhesive (susceptible) if ≥ 10% of examined brush borders bound > 2 bacteria.
  
  
  RESULTS
  Most purebred and crossbred pigs had nonadhesive phenotypes with regard to ETEC K99 and 987P strains. For the F41 strain, 34.9% and 65.1% of all purebred pigs had adhesive and nonadhesive phenotypes, respectively; among crossbred pigs, these values were 39.2% and 60.8%, respectively. The percentage of pigs with the F41 adhesive phenotype was higher among Western breeds than it was among Chinese breeds (38.9% vs 31.1%).
  
  
  CONCLUSIONS
  Results suggested that the ETEC F41 strain, but not the K99 or 987P strain, might be a cause of diarrhea in 6- to 8-week-old pigs in China.
authors.affiliation: Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang 330045, China.
authors.forename: Xueming
authors.initials: X
authors.lastname: Yan
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Jing
authors.initials: J
authors.lastname: Ouyang
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Han
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D001947:Breeding; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D020022:Genetic Predisposition to Disease; D007583:Jejunum / Q000473:pathology; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000382:microbiology*
chemical_list: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; C083561:F41 antigen, E coli; C031429:K99 antigen; C035391:antigen 987P, E coli; D036781:Fimbriae Proteins