title: [Differentiation of human fetal liver CD34+ cells into neuronal cells induced by beta-ME and BHA in vitro].
abstract: OBJECTIVE
  To establish model of differentiation of fetal liver stem cells induced by beta-ME + BHA into neural cells in vitro;
  
  
  METHODS
  CD34+ cells from naturally aborted human fetal liver were isolated with MACS Kit, and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS). After confluent more than 80%, the 5 passage cells were induced by 10(-3) mol/L beta-mercaptoethanol (beta-ME) and 2 x 10(-4) mol/L BHA for 24 hours, and washed with PBS, and then incubated in serum-free medium for 5 hours to 5 days. The characteristics of treated cells were assayed by immunocytochemistry staining analysis.
  
  
  RESULTS
  Cells treated by beta-ME+ BHA exhibited neuronal phenotype, and expressed neuronal specific proteins such as nestin, NeuN, TrnJ-1, and NF-M, which were not found in control cells. Statistic analysis showed that 81% cells were NeuN-positive, 75% cells TuJ-1-positive, 47% cells NF-M-positive, 90% cells NSE-positive.
  
  
  CONCLUSIONS
  beta-ME + BHA can induce human fetal liver CD34+ cells to produce neuronal specific antigens and proteins in vitro and become neuronal cells. CD34+ cells from human fetal liver possess potentials of differentiation into neural cells.
authors.affiliation: Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, China.
authors.forename: Ge-xiu
authors.initials: GX
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D018952:Antigens, CD34; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology; D008623:Mercaptoethanol / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology
chemical_list: D018952:Antigens, CD34; D002083:Butylated Hydroxyanisole; D008623:Mercaptoethanol