title: Biodegradation of fenvalerate and 3-phenoxybenzoic acid by a novel Stenotrophomonas sp. strain ZS-S-01 and its use in bioremediation of contaminated soils.
abstract: A bacterial strain ZS-S-01, newly isolated from activated sludge, could effectively degrade fenvalerate and its hydrolysis product 3-phenoxybenzoic acid (3-PBA). Based on the morphology, physiological biochemical characteristics, and 16 S rDNA sequence, strain ZS-S-01 was identified as Stenotrophomonas sp. Strain ZS-S-01 could also degrade and utilize deltamethrin, beta-cypermethrin, beta-cyfluthrin, and cyhalothrin as substrates for growth. Strain ZS-S-01 was capable of degrading fenvalerate rapidly without a lag phase over a wide range of pH and temperature, even in the presence of other carbon sources, and metabolized it to yield 3-PBA, then completely degraded it. No persistent accumulative product was detected by HPLC and GC/MS analysis. Studies on biodegradation in various soils showed that strain ZS-S-01 demonstrated efficient degradation of fenvalerate and 3-PBA (both 50 mg·kg(-1)) with a rate constant of 0.1418-0.3073 d(-1), and half-lives ranged from 2.3 to 4.9 days. Compared with the controls, the half-lives for fenvalerate and 3-PBA reduced by 16.9-156.3 days. These results highlight strain ZS-S-01 may have potential for use in bioremediation of pyrethroid-contaminated environment.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, People's Republic of China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Meiying
authors.initials: M
authors.lastname: Hu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
mesh_terms: D001565:Benzoates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D009570:Nitriles / Q000378:metabolism*; D011722:Pyrethrins / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D020588:Stenotrophomonas / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D001565:Benzoates; D004269:DNA, Bacterial; D009570:Nitriles; D011722:Pyrethrins; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012989:Soil Pollutants; C017160:cypermethrin; C017180:decamethrin; C017618:3-phenoxybenzoic acid; C052570:cyfluthrin; C037304:cyhalothrin; C017690:fenvalerate