title: Multiple expression of glucose transporters in the lateral wall of the cochlear duct studied by quantitative real-time PCR assay.
abstract: We have investigated the gene expression of the facilitated glucose transporter (GLUT), H+-coupled myo-inositol cotransporter (HMIT), and Na+ glucose cotransporter (SGLT) in the lateral wall of the cochlear duct by conventional RT-PCR and quantitative real-time PCR. The isoforms GLUT1, -3, -4, -5, -8, -10, -12 and HMIT were detected in both the stria vascularis and the spiral ligament, whereas no SGLT isoforms could be detected in these tissues. Quantitative real-time PCR analysis revealed significant differences in the gene expression of GLUT1, -4, -5, -10, and HMIT isoforms between the stria vascularis and the spiral ligament. This result reflects the tissue-dependent distributions of GLUT isoforms. These findings strongly suggest that a number of GLUT isoforms participate in glucose transport in the stria vascularis and the spiral ligament.
authors.affiliation: Laboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama University, Okayama 700-8530, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Edamatsu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kondo
authors.forename: Motonori
authors.initials: M
authors.lastname: Ando
mesh_terms: D000818:Animals; D003053:Cochlear Duct / Q000033:anatomy & histology* / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013316:Stria Vascularis / Q000378:metabolism*
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D020033:Protein Isoforms; C486673:Slc2a13 protein, rat