title: Loss of striatal dopaminergic terminals during the early stage in response to MPTP injection in C57BL/6 mice.
abstract: The molecular mechanisms underlying MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-induced dopaminergic (DAergic) neuronal death in vivo are still not fully understood. To investigate the selective DAergic neurotoxicity, we have developed an immunological technique to isolate DAergic synaptosomes from mouse striatal tissues using an antibody against 20 amino acid residues in the extracellular second loop of dopamine transporter (DAT). The DAT protein level in the isolated DAergic synaptosomes was markedly decreased at 16 h after a single injection of 30 mg/kg MPTP, but not in striatal homogenate and crude synaptosomes fraction. GBR-12909, a dopamine uptake inhibitor, completely reversed the MPTP-induced decrease of DAT protein in the DAergic synaptosomes. These results suggest that the isolated DAergic synaptosomes can be useful to identify mechanisms of loss of the nerve terminals.
authors.affiliation: Laboratory of Alternative Medicine and Experimental Therapeutics, Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Ishikawa 920-1181, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Muroyama
authors.forename: Shota
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Mitsumoto
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology*; D004298:Dopamine / Q000378:metabolism*; D020267:MPTP Poisoning / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017729:Presynaptic Terminals / Q000378:metabolism / Q000473:pathology*; D013574:Synaptosomes / Q000378:metabolism / Q000473:pathology*
chemical_list: D004298:Dopamine