title: Interleukin-8 is associated with adhesion, migration and invasion in human gastric cancer SCG-7901 cells.
abstract: Interleukin-8 is known as an important chemokine involved in tumor angiogenesis and progression. Overexpression of interleukin-8 has been detected in a variety of human tumors, including gastric cancer, and is negatively correlated with prognosis. The aim of our study is to determine the effects of interleukin-8 on proliferation, adhesion, migration and invasion abilities and correlated molecular mechanisms in gastric cancer. We made recombinant interleukin-8 ranged from 0 ng/ml to 100 ng/ml interferes in human gastric cancer SCG-7901 cells in vitro. The results shown that interleukin-8 did not change cell proliferation, but promoted cell adhesion to endothelial cell and extracellular matrix components (collagen, laminin and fibronectin) as detected by Cell Counting Kit-8. And it induced migration and invasion ability based on scratch and transwell-chamber assays. Also, interleukin-8 regulated the protein and mRNA expression of matrix metalloproteinase-9, intercellular adhesion molecule-1 and E-cad and there was obviously a dose-dependent relationship, but the protein or mRNA expression of matrix metalloproteinase-2 was not obviously changed under the tested conditions. Our findings indicate that interleukin-8 is associated with adhesion, migration and invasion in gastric cancer and the regulation of matrix metalloproteinase-9, intercellular adhesion molecule-1 and E-cad expression is one of the potential molecule mechanisms. The studies imply interleukin-8 may be an alternative treatment strategy against gastric cancer.
authors.affiliation: Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, 415 Fengyang Avenue, 200003 Shanghai, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Ju
authors.forename: Dazhi
authors.initials: D
authors.lastname: Sun
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xiu
authors.forename: Xianze
authors.initials: X
authors.lastname: Meng
authors.forename: Cian
authors.initials: C
authors.lastname: Zhang
authors.forename: Pinkang
authors.initials: P
authors.lastname: Wei
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000096:biosynthesis*; D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D015820:Cadherins; D016209:Interleukin-8; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D020780:Matrix Metalloproteinase 9