title: Detection and characterization of a novel polyomavirus in wild rodents.
abstract: To investigate polyomavirus infection in wild rodents, we analysed DNA samples from the spleens of 100 wild rodents from Zambia using a broad-spectrum PCR-based assay. A previously unknown polyomavirus genome was identified in a sample from a multimammate mouse (Mastomys species) and the entire viral genome of 4899 bp was subsequently sequenced. This viral genome contained potential ORFs for the capsid proteins, VP1, VP2 and VP3, and early proteins, small t antigen and large T antigen. Phylogenetic analysis showed that it was a novel member of the family Polyomaviridae, and thus the virus was tentatively named mastomys polyomavirus. After transfection of the viral genome into several mammalian cell lines, transient expression of the VP1 and large T antigen proteins was confirmed by immunoblotting and immunocytochemical analyses. Comparison of large T antigen function in mastomys polyomavirus with that in rhesus monkey polyomavirus SV40 and human polyomavirus JC virus revealed that the large T antigen from mastomys polyomavirus interacted with the tumour suppressor protein pRb, but not with p53.
authors.affiliation: Department of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Orba
authors.forename: Shintaro
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakamura
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ishii
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Hang'ombe
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Mweene
authors.forename: Yuka
authors.initials: Y
authors.lastname: Thomas
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawa
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D000914:Antibodies, Viral / Q000276:immunology; D016000:Cluster Analysis; D003429:Cross Reactions; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D015024:Zambia
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D014764:Viral Proteins