title: [Effects of hypoxic-preconditioning on anoxic-tolerance and Jun expression in cultured rat hippocampal neurons].
abstract: OBJECTIVE
  To study the effects of hypoxic preconditioning on anoxic tolerance and Jun expression in cultured rat hippocampal neurons after anoxia/reoxygenation.
  
  
  METHODS
  12 day cultured hippocampal neurons in control and hypoxic preconditioning group were exposed to anoxic environment (0.90L/L N2 + 0.10 L/L CO2) for 4 h, and then reoxygenated for either 24 h or 72 h. The neurons were immunocytochemically stained using the antiserum against Jun. The number of survival neurons and the percentage of Jun expressing neurons were investigated.
  
  
  RESULTS
  The percentage of Jun expressing neurons induced by anoxia in hypoxic-preconditioning group was significantly less than that in control group. The number of survival neurons was more in the hypoxic-preconditioning group than that in control group after anoxic reoxygenation.
  
  
  CONCLUSIONS
  Hypoxic-preconditioning can induce the development of anoxic-tolerance in cultured hippocampal neurons. The decrease in Jun expressing neurons in hippocampus may be an adaptive reaction to acute anoxia.
authors.affiliation: Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ai-Shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Fu-Zhuang
authors.initials: FZ
authors.lastname: Wang
authors.forename: Shun
authors.initials: S
authors.lastname: Yu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016758:Genes, jun; D006624:Hippocampus / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010100:Oxygen