title: Protective effect of bicyclol on lipopolysaccharide-induced acute lung injury in mice.
abstract: Bicyclol is synthesized based on schisandrin, which is one of the main active components of Chinese herb Fructus Schisandrae. The purpose of this study is to investigate whether bicyclol has a beneficial effect on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Bicyclol was given to mice by gavage for three times. ALI was induced by vena caudalis injection of LPS. The last dose of bicyclol was administrated 1 h before LPS given. Mice in each group were sacrificed at different time point after LPS administration. As revealed by survival study, pretreatment with high doses of bicyclol reduced the mortality of mice from ALI. Bicyclol pretreatment significantly improved LPS-induced lung pathological changes, inhibited myeloperoxidase (MPO) activity, and reduced lung/body and lung wet/dry weight ratios. Bicyclol also inhibited the release of TNF-α, IL-1β and HMGB1, whereas simultaneously increased the expression of IL-10. Furthermore, the phosphorylation level of NF-κB p65 was markedly decreased by bicyclol. Taken together, our study showed that bicyclol improves survival rate and attenuates LPS-induced ALI. The protective mechanism may be due to the inhibition of NF-κB activation and regulation of cytokine secretion.
authors.affiliation: Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China. luoying@fmmu.edu.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Dun-Quan
authors.initials: DQ
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Dong
authors.forename: Peng-Tao
authors.initials: PT
authors.lastname: Zhao
authors.forename: Zhi-Chao
authors.initials: ZC
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000401:mortality / Q000517:prevention & control*; D000818:Animals; D001713:Biphenyl Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051996:Transcription Factor RelA; C477843:bicyclol; D009195:Peroxidase