title: Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis.
abstract: We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for secondarily infected lesions (n = 8) were 100%. In primarily nonulcerative lesions (n = 15), the sensitivity of FPLI PCR was inferior to that of pooled-invasive-specimen PCR (72.7% versus 100%) (P = 0.10). FPLI PCR is sensitive, specific, and unlike invasive procedures, can be used in secondarily infected ulcers. Invasive specimen collection is superior in nonulcerative lesions.
authors.affiliation: Tropical Disease Unit, Toronto General Hospital, 200 Elizabeth Street, 13th Floor, North Wing, Room 1350, Toronto, ON M5G 1C4, Canada. andrea.boggild@utoronto.ca
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Boggild
authors.forename: Ana Pilar
authors.initials: AP
authors.lastname: Ramos
authors.forename: Braulio Mark
authors.initials: BM
authors.lastname: Valencia
authors.forename: Nicolas
authors.initials: N
authors.lastname: Veland
authors.forename: Flor
authors.initials: F
authors.lastname: Calderon
authors.forename: Jorge
authors.initials: J
authors.lastname: Arevalo
authors.forename: Donald E
authors.initials: DE
authors.lastname: Low
authors.forename: Alejandro
authors.initials: A
authors.lastname: Llanos-Cuentas
mesh_terms: D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012883:Skin Ulcer / Q000469:parasitology* / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D016054:DNA, Protozoan