title: Interferon regulatory factor 3-CL, an isoform of IRF3, antagonizes activity of IRF3.
abstract: Interferon regulatory factor 3 (IRF3), one member of the IRF family, plays a central role in induction of type I interferon (IFN) and regulation of apoptosis. Controlled activity of IRF3 is essential for its functions. During reverse transcription (RT)-PCR to clone the full-length open reading frame (ORF) of IRF3, we cloned a full-length ORF encoding an isoform of IRF3, termed as IRF3-CL, and has a unique carboxyl-terminus of 125 amino acids. IRF3-CL is ubiquitously expressed in distinct cell lines. Overexpression of IRF3-CL inhibits Sendai virus (SeV)-triggered induction of IFN-β and SeV-induced and inhibitor of NF-κB kinase-ε (IKKε)-mediated nuclear translocation of IRF3. When IKKε is overexpressed, IRF3-CL is associated with IRF3. These results suggest that IRF3-CL, the alternative splicing isoform of IRF-3, may function as a negative regulator of IRF3.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Li
authors.forename: Lixin
authors.initials: L
authors.lastname: Ma
authors.forename: Xinwen
authors.initials: X
authors.lastname: Chen
mesh_terms: D021581:Active Transport, Cell Nucleus; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D051550:I-kappa B Kinase / Q000235:genetics / Q000378:metabolism; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000378:metabolism; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism; D016899:Interferon-beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D029082:Sendai virus / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics
chemical_list: D050838:Interferon Regulatory Factor-3; D007370:Interferon Type I; D016328:NF-kappa B; D020033:Protein Isoforms; D016899:Interferon-beta; D051550:I-kappa B Kinase