title: Regioselective deglycosylation of onion quercetin glucosides by Saccharomyces cerevisiae.
abstract: Bioconversion of quercetin glucosides using four generally recognized as safe (GRAS) organisms (Aspergillus oryzae, Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) was evaluated by measuring changes in the levels of quercetin compounds of onion. Of the four organisms, S. cerevisiae increased the content of quercetin-3-O-β-D-glucoside (III; isoquercitrin) and quercetin (IV), whereas decreasing quercetin-3,4'-O-β-D-glucoside (I) and quercetin-4'-O-β-D-glucoside (II). Also, S. cerevisiae converted authentic compound I to III, and II to IV, respectively. These results suggest that S. cerevisiae can be used to increase the levels of isoquercitrin (III), the most bioavailable quercetin compound in onion.
authors.affiliation: Microbe-based Fusion Technology Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk, Republic of Korea.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Chung
authors.forename: Yung-Chul
authors.initials: YC
authors.lastname: Chung
authors.forename: Pil Je
authors.initials: PJ
authors.lastname: Maeng
authors.forename: Hyo-Kon
authors.initials: HK
authors.lastname: Chun
mesh_terms: D005960:Glucosides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D019697:Onions / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013237:Stereoisomerism; D015854:Up-Regulation
chemical_list: D005960:Glucosides; C016527:isoquercitrin; D011794:Quercetin