title: [Involvement of leukotrine B4 receptors in the inflammatory responses and immunological regulation in vitro].
abstract: OBJECTIVE
  BLT1 and BLT2 were both recently cloned and identified as two subtypes of leukotrine B4 (LTB4) receptors. With the usage of U-75302 and LY255283, the specific antagonists of BLT1 and BLT2 respectively, the involvement of BLT1 and BLT2 in the inflammatory and immunological responses was in vitro explored.
  
  
  METHODS
  (1) To investigate inhibition of U-75302 and LY255283 on the proliferation of rat synovial cells, 3H-TdR incorporation into the cells was quantified. (2) Flow cytometric assay for interferon-gamma (IFN-gamma) and interleukine 4 (IL-4) profiles in CD4+ T lymphocytes from rat spleen was carried out to determine the ratio of Th1/Th2.
  
  
  RESULTS
  (1) For inhibition on rat synovial cells proliferation, U-75302 exerted its effect only at a high concentration of 10 micromol/L and LY255283 at the concentrations of 10 micromol/L-10 micromol/L. (2) Both U-75302 and LY255283 could elevate the percentage of Th2, but could not influence that of Th1.
  
  
  CONCLUSIONS
  BLT1 and BLT2 were involved in the synovial cells proliferation change the ratio of Th1/Th2. Their meaning served as targets for prevention and treatment of infectious diseases should be emphasized.
authors.affiliation: Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Chun-guang
authors.initials: CG
authors.lastname: Han
authors.forename: Huo-gao
authors.initials: HG
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Luo
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.forename: Yong-xue
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology; D006018:Glycols / Q000494:pharmacology; D007249:Inflammation / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018102:Receptors, Leukotriene B4 / Q000037:antagonists & inhibitors / Q000502:physiology*; D013583:Synovial Membrane / Q000166:cytology / Q000276:immunology*; D013777:Tetrazoles / Q000494:pharmacology; D058408:Th1-Th2 Balance
chemical_list: D005233:Fatty Alcohols; D006018:Glycols; D018102:Receptors, Leukotriene B4; D013777:Tetrazoles; C062137:U 75302; C063823:LY 255283