title: Establishment of transgenic mice carrying the gene for farnesyl pyrophosphate synthase.
abstract: Farnesyl pyrophosphate synthase (FPPS) is an essential enzyme in the mevalonate pathway and might be relevant to hypertension and other cardiovascular diseases. FPPS transgenic mice were produced by microinjecting a construct with the FPPS gene into fertilized eggs derived from an inbred C57BL/6 strain. Three mice were identified as carrying copies of the transgene using the PCR. Reverse transcription PCR and Western blotting showed that the transgene was expressed in heart, liver, lung, ear, brain, thymus, and blood vessels in the transgenic mouse. Pathological analysis (hematoxylin and eosin staining) showed that FPPS expression did not cause obvious pathological changes in multiple tissues of 6-week-old transgenic mice. This FPPS transgenic mouse model, may therefore, facilitate the investigation of the biological functions of FPPS in vivo.
authors.affiliation: College of Medicine, Institute of Cardiology, The First Affiliated Hospital, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Jun-Chang
authors.initials: JC
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Shen-Jiang
authors.initials: SJ
authors.lastname: Hu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004122:Dimethylallyltranstransferase / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004122:Dimethylallyltranstransferase