title: Anti-adenovirus humoral responses influence on the efficacy of vaccines based on epitope display on adenovirus capsid.
abstract: The efficacy of recombinant adenoviruses (Ads) vaccine vectors is diminished by the high prevalence of anti-Ad antibodies (Abs) that hampers gene transfer. Epitope display on Ad capsid constitutes an alternative approach to bypass anti-Ad Ab capacity from blocking antigen expression. To understand the role of the epitope insertion site, an ovalbumin-derived epitope was genetically inserted into either Ad hexon or fiber proteins. Hexon-modified Ads triggered higher anti-ovalbumin Ab responses after one injection but surprisingly fiber-modified Ads were by far more potent after two or several administrations. Our data unravel a role for anti-Ad humoral immunity in controlling anti-epitope humoral responses.
authors.affiliation: CNRS UMR 8203, Vectorologie et thérapeutiques anticancéreuses, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Lanzi
authors.forename: Ghada
authors.initials: G
authors.lastname: Ben Youssef
authors.forename: Michel
authors.initials: M
authors.lastname: Perricaudet
authors.forename: Karim
authors.initials: K
authors.lastname: Benihoud
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; C018315:hexon capsid protein, Adenovirus; D010047:Ovalbumin