title: [Changes of the elastic fibers and collagen fibers during the development and progression of experimentally induced tongue carcinoma in hamsters].
abstract: OBJECTIVE
  To investigate the relationship between the extracellular matrix (ECM) and neoplastic progression in hamster with tongue cancer.
  
  
  METHODS
  Forty-eight specimens of hamster tongue cancer were divided into control group (n=6) and experimental group (n=42). The pathological grade of the specimens was assessed (including 3 stages, namely atypical hyperplasia, carcinoma in situ and early invasive carcinoma). The sections of the tongue were stained with Masson and aldehyde-fuchsin (AF) staining for microscopic observation of the elastic fiber and collagen fiber changes.
  
  
  RESULTS
  Within the connective tissue cores (CTC) of the papillae in the control group was a framework of numerous and fine Gomrori's aldehyde fuchsin-positive elastic fibers. But in the stages of dysplasia and carcinoma in situ, these elastic fibers decreased and further diminished in the CTC in early invasive carcinoma. In dysplasia and carcinoma in situ stages, most of the elastic fibers collapsed with scattered elastic fibers, and the elastic fibers decreased significantly in early invasive carcinoma. The control group showed a significantly greater number of elastic fibers in the experimental group. The collagen fiber was obviously increased and irregularly arranged in dysplasia and carcinoma in situ stage; in early invasive carcinoma, the collagen fibers became thicker with deposition in the lamina propria.
  
  
  CONCLUSIONS
  An excessive deposition of collagen fiber and reduction of the elastic fibers is an important factor contributing to the development of tongue carcinoma in hamsters.
authors.affiliation: Department of Anatomy, Harbin Medical University, China. zhengpenghrbmu2009@163.com
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yu-bin
authors.initials: YB
authors.lastname: DU
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Xiang
authors.initials: X
authors.lastname: Yin
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Lv
authors.forename: Zhong-xiu-zi
authors.initials: ZX
authors.lastname: Gao
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D003238:Connective Tissue / Q000473:pathology*; D006224:Cricetinae; D004547:Elastic Tissue / Q000473:pathology*; D005109:Extracellular Matrix / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000473:pathology; D014062:Tongue Neoplasms / Q000473:pathology*
chemical_list: D003094:Collagen