title: [First detection of human astrovirus in gastroenteritis outbreak in Hungary].
abstract: Human astroviruses are one of the known pathogens of gastroenteritis in infants, children and rarely in elderly. It causes 4.2-7.3% of the sporadic gastroenteritis cases with diarrhea and vomiting in children. The etiological role of astrovirus has not been confirmed yet in outbreaks of gastroenteritis in Hungary.
  
  
  OBJECTIVE
  The first description of the detection and molecular epidemiology of astrovirus in outbreaks of gastroenteritis in Hungary.
  
  
  METHODS
  Stool samples originated from Komárom-Esztergom County, from a day-care center (nursery) where a gastroenteritis outbreak occurred in June, 2010. Astrovirus was detected by RT-PCR methods. The nucleotide sequence of the nearly complete genome was sequenced. Clinical and epidemiological data were collected by epidemiological investigation.
  
  
  RESULTS
  Out of the 29 exposed persons (24 children and 5 adults) 7 (24.1%) children had gastroenteritis with diarrhea, and vomiting in one case, in the period of June 4-15, 2010. Bacterial pathogens, rotavirus, adenovirus and norovirus were not detected, but genotype 1 astrovirus could be identified in 3 (42.8%) stool samples (HQ398856). The nucleotide sequence of the astrovirus ORF1a/ORF1b/ORF2/3'UTR regions was determined. The source of the outbreak was presumably the firstly recognized ill child and the virus was spread by fecal-oral route with direct contact in the children community.
  
  
  CONCLUSIONS
  Epidemiologic and clinical characteristics of the astrovirus outbreak in the nursery are described in details to prove that the possible etiological role of astroviruses in viral gastroenteritis which should not forget in order after rotaviruses, caliciviruses (norovirus and sapovirus) and enteric adenoviruses.
authors.affiliation: Állami Népegészségügyi és Tisztiorvosi Szolgálat Dél-dunántúli Regionális Intézete, Regionális Virológiai Laboratórium, Gastroenteralis Vírusok Nemzeti Referencia Laboratóriuma, Pécs.
authors.forename: Péter
authors.initials: P
authors.lastname: Pankovics
authors.forename: Ákos
authors.initials: Á
authors.lastname: Boros
authors.forename: Melinda
authors.initials: M
authors.lastname: Rovács
authors.forename: Erika
authors.initials: E
authors.lastname: Nagy
authors.forename: Erika
authors.initials: E
authors.lastname: Krisztián
authors.forename: Mária
authors.initials: M
authors.lastname: Vollain
authors.forename: Gábor
authors.initials: G
authors.lastname: Reuter
mesh_terms: D019350:Astroviridae Infections / Q000453:epidemiology* / Q000821:virology; D002656:Child Day Care Centers / Q000706:statistics & numerical data*; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D003967:Diarrhea / Q000821:virology; D004196:Disease Outbreaks / Q000706:statistics & numerical data; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D007223:Infant; D008297:Male; D001257:Mamastrovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014839:Vomiting / Q000821:virology
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral