title: Black soybean extract can attenuate thrombosis through inhibition of collagen-induced platelet activation.
abstract: Many clinical trials have demonstrated the beneficial effects of soybean (Glycine max) on general cardiovascular health. Among a variety of soybeans, black soybean is known to display diverse biological activities superior to those of yellow and green soybeans, such as in antioxidant, anti-inflammatory and anticancer activities. However, few studies have been directed on the effect of black soybean on cardiovascular function. In this study, we aimed to investigate the effect of black soybean extract (BB) on platelet activation, a key contributor to thrombotic diseases. In freshly isolated human platelets, BB has shown potent inhibitory activity on collagen-induced platelet aggregation, while yellow soybean extract had marginal activity only. BB also attenuated serotonin secretion and P-selectin expression, which are important factors for the platelet-tissue interaction along with thromboxane A(2) formation. These in vitro results were further confirmed in an ex vivo platelet aggregation measurement and in vivo venous thrombosis model where oral administration of BB reduced collagen-induced platelet aggregation and FeCl(3)-induced thrombus formation significantly. A potential active ingredient for antiplatelet effects of BB was isolated and identified to be adenosine through bioassay-directed fractionation and NMR and ESI-MS analyses. These results indicate that black soybean can be a novel dietary supplement for the prevention of cardiovascular risks and the improvement of blood circulation.
authors.affiliation: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, South Korea.
authors.forename: Keunyoung
authors.initials: K
authors.lastname: Kim
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Lim
authors.forename: Chae-Wook
authors.initials: CW
authors.lastname: Kim
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Shin
authors.forename: Dae-Bang
authors.initials: DB
authors.lastname: Seo
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Ji-Yoon
authors.initials: JY
authors.lastname: Noh
authors.forename: Ok-Nam
authors.initials: ON
authors.lastname: Bae
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D006801:Humans; D008297:Male; D019007:P-Selectin / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D015539:Platelet Activation / Q000187:drug effects*; D012701:Serotonin / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013927:Thrombosis / Q000097:blood / Q000517:prevention & control*
chemical_list: D019007:P-Selectin; D010936:Plant Extracts; D012701:Serotonin; D003094:Collagen