title: Synthesis, spectroscopic study and anticancer activity of a water-soluble Nb(V) peroxo complex.
abstract: We synthesized, characterized and studied the anticancer properties of a new water-soluble peroxo niobium complex (K(3)[Nb(Asc)(O(2))(3)]·4H(2)O (Asc = ascorbate anion C(6)H(6)O(6)(2-))), as well as that of ascorbic acid, in human leukemic cells. The complex was synthesized and characterized by elemental, IR, Raman, thermogravimetric analysis, detailed NMR and mass spectra analysis. The cytotoxic activity of the complex on HL-60 and K562 human leukemia cell lines has been investigated by assessing vital cellular mechanisms, such as the metabolic activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MTT) and the proliferation capacity (growth curves) of leukemia cells, as well as the structural integrity of cell membrane (trypan blue assay). The complex exerts an increased antiproliferative effect primarily on HL60 human leukemia cells, compared to ascorbic acid alone, as well as an inhibitory effect on necrosis caused by ascorbic acid. Its effect on K562 cells concerns mainly its inhibitory effect upon cell necrosis induced by ascorbic acid alone. Our results support a concentration- and time-dependent enhanced antileukemic effect of the complex, suggesting its significance as a promising tool in the confrontation of leukemia.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Athens, Panepistimiopolis, 15701 Athens, Greece.
authors.forename: Hellinida
authors.initials: H
authors.lastname: Thomadaki
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lymberopoulou-Karaliota
authors.forename: Adamantia
authors.initials: A
authors.lastname: Maniatakou
authors.forename: Andreas
authors.initials: A
authors.lastname: Scorilas
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018360:Crystallography, X-Ray; D015195:Drug Design; D055808:Drug Discovery; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D009556:Niobium; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman; D013818:Thermogravimetry
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009556:Niobium; D001205:Ascorbic Acid