title: [Rat in vitro plasma-binding percentage determination of sinomenine hydrochloride by using microdialysis method and ultrafiltration].
abstract: OBJECTIVE
  To assess feasibility of plasma protein-binding percentage by using microdialysis method.
  
  
  METHODS
  Sinomenine hydrochloride was selected as model drug. The in vitro rat plasma protein-binding percentage of three kinds of concentration was determinated by using microdialysis sampling tool and HPLC-UV. At the same time the classical study method, such as ultrafiltration method, was also used to determinate the protein-binding percentage to compare the difference between the two methods.
  
  
  RESULTS
  The in vitro rat plasma protein-binding percentage of sinomenine hydrochloride using microdialysis method was about 26% and kept relative stable at the concentration range. There was no significant difference between the two methods.
  
  
  CONCLUSIONS
  The results shows that the binding percentage is relative low. Microdialysis method is a new method for the study of protein-binding degree.
authors.affiliation: Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China. zyfeng-2006@163.com
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liling
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D017551:Microdialysis / Q000379:methods*; D009019:Morphinans / Q000097:blood* / Q000493:pharmacokinetics*; D010949:Plasma / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology*; D051381:Rats; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D001798:Blood Proteins; D009019:Morphinans; C009271:sinomenine