title: Resin replica in enamel deproteinization and its effect on acid etching.
abstract: OBJECTIVE
  The goal of this in vitro study was to identify the topographical features of deproteinized (NaOCl) and etched with phosphoric acid (H3PO4) enamel surface, compared to phosphoric acid surface alone with a Resin Replica model.
  
  
  METHODS
  Ten extracted lower first and second permanent molars were polished with pumice and water, and then divided into 3 equal buccal sections having similar physical and chemical properties. The enamel surfaces of each group were subjected to the following treatments: Group A: Acid Etching with H3PO4 37% for 15 seconds. Group B: Sodium Hypochlorite (NaOCl) 5.25% for 60 seconds followed by Acid Etching with H3PO4 37% for 15 seconds. Group C; No treatment (control). All the samples were treated as follow: Adhesive and resin were applied to all groups after A, B and C treatment were performed; Then enamel/dentin decalcification and deproteinization and topographic SEM Resin Replica assessment were used to identify resin tags enamel surface quality penetration.
  
  
  RESULTS
  Showed that group B reached an area of 7.52 mm of the total surface, with a 5.68 mm2 (73%) resin tag penetration equivalent type I and II etching pattern, 1.71 mm2 (26%) equivalent to type III etching pattern and 0.07 mm2 (1%) unaffected surface. Followed by group A with 7.48 mm2 of the total surface, with a 3.47 mm2 (46 %)resin tag penetration equivalent to type I and II etching pattern, 3.30 mm2 (45%)equivalent to type III etching pattern and 0.71 mm2, and (9%) unaffected surface. Group C did not show any resin tag penetration. A significant statistical diference (P < 0.001) existed between groups A and B in resin quality penetration, leading to the conclusion that when the enamel is deproteinizated with 5.25% NaOCl for 1 minute prior H3PO4, the surface and topographical features of the replica resin penetration surface increases significantly with type I-II etching pattern.
authors.affiliation: Department of Oral Rehabilitation, Health Science and Environmental Centre, Universidad de Guadalajara, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Espinosa
authors.forename: Roberto
authors.initials: R
authors.lastname: Valencia
authors.forename: Mario
authors.initials: M
authors.lastname: Uribe
authors.forename: Israel
authors.initials: I
authors.lastname: Ceja
authors.forename: J
authors.initials: J
authors.lastname: Cruz
authors.forename: Marc
authors.initials: M
authors.lastname: Saadia
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry; D003743:Dental Enamel / Q000648:ultrastructure*; D003746:Dental Enamel Proteins / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry; D003804:Dentin / Q000648:ultrastructure*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010756:Phosphoric Acids / Q000737:chemistry; D011489:Protein Denaturation; D016628:Replica Techniques; D012973:Sodium Hypochlorite / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors
chemical_list: D003188:Composite Resins; D003738:Dental Cements; D003746:Dental Enamel Proteins; D003764:Dental Materials; D010756:Phosphoric Acids; C533204:filtek Z350; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D012973:Sodium Hypochlorite; C030242:phosphoric acid