title: Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease.
abstract: Paget's disease (PD) is characterized by abnormal osteoclasts (OCL) that secrete high IL-6 levels and induce exuberant bone formation. Because measles virus nucleocapsid gene (MVNP) and the p62(P392L) mutation are implicated in PD, marrows from 12 PD patients harboring p62(P392L) and eight normals were tested for MVNP expression and pagetic OCL formation. Eight out of twelve patients expressed MVNP and formed pagetic OCL in vitro, which were inhibited by antisense-MVNP. Four out of twelve patients lacked MVNP and formed normal OCL that were hyperresponsive to RANKL but unaffected by antisense-MVNP. Similarly, mice expressing only p62(P394L) formed normal OCL, while mice expressing MVNP in OCL, with or without p62(P394L), developed pagetic OCL and expressed high IL-6 levels dependent on p38MAPK activation. IL-6 deficiency in MVNP mice abrogated pagetic OCL development in vitro. Mice coexpressing MVNP and p62(P394L) developed dramatic Paget's-like bone lesions. These results suggest that p62(P394L) and IL-6 induction by MVNP play key roles in PD.
authors.affiliation: University of Pittsburgh, Medicine/Hem-Onc, Pittsburgh, PA 15213, USA.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Kurihara
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hiruma
authors.forename: Kei
authors.initials: K
authors.lastname: Yamana
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Michou
authors.forename: Côme
authors.initials: C
authors.lastname: Rousseau
authors.forename: Jean
authors.initials: J
authors.lastname: Morissette
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Galson
authors.forename: Jumpei
authors.initials: J
authors.lastname: Teramachi
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.forename: David W
authors.initials: DW
authors.lastname: Dempster
authors.forename: Jolene J
authors.initials: JJ
authors.lastname: Windle
authors.forename: Jacques P
authors.initials: JP
authors.lastname: Brown
authors.forename: G David
authors.initials: GD
authors.lastname: Roodman
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D001842:Bone and Bones / Q000473:pathology; D002117:Calcitriol / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008459:Measles virus / Q000235:genetics* / Q000472:pathogenicity; D051379:Mice; D008822:Mice, Transgenic; D008875:Middle Aged; D009154:Mutation; D019251:Nucleocapsid / Q000235:genetics; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000235:genetics; D010001:Osteitis Deformans / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology*; D053245:RANK Ligand / Q000494:pharmacology; D000071456:Sequestosome-1 Protein; D035381:TATA-Binding Protein Associated Factors / Q000096:biosynthesis; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015850:Interleukin-6; D019590:Nucleocapsid Proteins; D053245:RANK Ligand; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; C470012:TAF12 protein, human; D035381:TATA-Binding Protein Associated Factors; D048051:p38 Mitogen-Activated Protein Kinases; D002117:Calcitriol