title: A perfusion bioreactor for engineering bone constructs: an in vitro and in vivo study.
abstract: A perfusion bioreactor, which was designed based on fluidized bed concepts, was validated for the culture of bone constructs of clinically relevant size. For this study, natural coral has been used as three-dimensional scaffolds. This biomaterial is a microporous, biocompatible, osteoconductive, and absorbable scaffold. This perfusion bioreactor provided a stable environment in terms of osmolarity, pH, and, most importantly, oxidative stress. Bone constructs engineered in this system resulted in significantly higher cell proliferation and homogenous cell distribution than those cultured under static conditions. Particularly relevant to the production of bioengineered bone in a clinical setting, custom-made bone constructs (each one with volume up to 30 cm(3)) could be produced using a such perfusion bioreactor. Last, but not least, the bone constructs of clinically relevant volume thus produced were shown to be osteogenic when transplanted subcutaneously in sheep.
authors.affiliation: Laboratoire Mécanique des Sols, Structures et Matériaux, UMR CNRS 8579, École Centrale Paris, Châtenay-Malabry Cedex, France. bertrand.david@ecp.fr
authors.forename: Bertrand
authors.initials: B
authors.lastname: David
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnefont-Rousselot
authors.forename: Karim
authors.initials: K
authors.lastname: Oudina
authors.forename: Marie-Christelle
authors.initials: MC
authors.lastname: Degat
authors.forename: Mickael
authors.initials: M
authors.lastname: Deschepper
authors.forename: Véronique
authors.initials: V
authors.lastname: Viateau
authors.forename: Morad
authors.initials: M
authors.lastname: Bensidhoum
authors.forename: Christian
authors.initials: C
authors.lastname: Oddou
authors.forename: Hervé
authors.initials: H
authors.lastname: Petite
mesh_terms: D000641:Ammonia / Q000032:analysis; D000818:Animals; D019149:Bioreactors; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D010477:Perfusion / Q000295:instrumentation*; D012212:Rheology; D012756:Sheep; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*
chemical_list: D003470:Culture Media; D049452:Green Fluorescent Proteins; D019344:Lactic Acid; D000641:Ammonia