title: Chondrogenic differentiation of human bone marrow mesenchymal stem cells on polyhydroxyalkanoate (PHA) scaffolds coated with PHA granule binding protein PhaP fused with RGD peptide.
abstract: Hydrophobic polyhydroxyalkanoate (PHA) scaffolds made of a copolyester of 3-hydroxybutyrate-co-hydroxyhexanoate (PHBHHx) were coated with a fusion protein PHA granule binding protein PhaP fused with RGD peptide (PhaP-RGD). Human bone marrow mesenchymal stem cells (hBMSCs) were inoculated on/in the scaffolds for formation of articular cartilages derived from chondrogenic differentiation of hBMSCs for cartilage tissue engineering. PhaP-RGD coating led to more homogeneous spread of cells, better cell adhesion, proliferation and chondrogenic differentiation in the scaffolds compared with those of PhaP coated or uncoated scaffolds immerging in serum minus chondrogenic induction medium. In addition, more extracellular matrices were produced by the differentiated cells over a period of 14 days on/in the PhaP-RGD coated scaffolds evidenced by scanning electron microscopy imaging, enhanced expression of chondrocyte specific genes including SOX-9, aggrecan and type II collagen, suggesting the positive effect of RGD on extracellular matrix production. Furthermore, cartilage-specific extracellular substances sulphated glycosaminoglycans (sGAG) and total collagen content found on/in the PhaP-RGD coated scaffolds were significantly more compared with that produced by the control and PhaP only coated scaffolds. Homogeneously distributed chondrocytes-like cells forming cartilage-like matrices were observed on/in the PhaP-RGD coated scaffolds after 3 weeks. The results suggested that PhaP-RGD coated PHBHHx scaffold promoted chondrogenic differentiation of hBMSCs and could support cartilage tissue engineering.
authors.affiliation: Multidisciplinary Research Center, Shantou University, Shantou 515063, Guangdong, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: You
authors.forename: Gongfeng
authors.initials: G
authors.lastname: Peng
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Ma
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Wang
authors.forename: Weiliang
authors.initials: W
authors.lastname: Shu
authors.forename: Siwu
authors.initials: S
authors.lastname: Peng
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002648:Child; D020219:Chondrogenesis / Q000187:drug effects* / Q000235:genetics; D003094:Collagen / Q000378:metabolism; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000494:pharmacology*; D009928:Organ Specificity / Q000187:drug effects; D054813:Polyhydroxyalkanoates / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D015203:Reproducibility of Results; D054457:Tissue Scaffolds / Q000737:chemistry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D006025:Glycosaminoglycans; D009842:Oligopeptides; D054813:Polyhydroxyalkanoates; D011993:Recombinant Fusion Proteins; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen