title: Characterization of immortalized mesenchymal stem cells derived from foetal porcine pancreas.
abstract: Islet replacement therapy is limited by shortage of donor islet cells. Usage of islet cells derived from porcine pancreatic stem cells (PSCs) is currently viewed as the most promising alternative for human islet transplantation. However, PSCs are rare and have a finite proliferative lifespan. In this study, we isolated and established an immortalized mesenchymal stem cell (MSC) line derived from foetal porcine pancreas, by transfecting human telomerase reverse transcriptase (hTERT) and called these immortalized pancreatic mesenchymal stem cells (iPMSCs). The iPMSCs have been cultured for more than 80 passages and have capacity to differentiate into neurons, cardiomyocytes, germ cells and islet-like cells, analysed by morphology, RT-PCR, western blotting, immunofluorescence, immunocytochemistry and transplantation assay. Islets derived from iPMSCs reversed hyperglycaemia in streptozotocin-induced diabetic mice and secreted insulin and C-peptide in vitro. These results demonstrated that iPMSCs might provide unlimited resources for islet replacement therapy and models for functional cell differentiation.
authors.affiliation: College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Key Lab for Reproductive Physiology and Embryo Biotechnology of Agriculture Ministry of China, Shaanxi, China.
authors.forename: H
authors.initials: H
authors.lastname: Cao
authors.forename: Y
authors.initials: Y
authors.lastname: Chu
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Pu
authors.forename: Z
authors.initials: Z
authors.lastname: Gao
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Peng
authors.forename: Z
authors.initials: Z
authors.lastname: Dou
authors.forename: J
authors.initials: J
authors.lastname: Hua
mesh_terms: D000818:Animals; D002096:C-Peptide / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D002461:Cell Line, Transformed; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D005333:Fetus / Q000166:cytology; D006801:Humans; D006943:Hyperglycemia / Q000097:blood; D007328:Insulin / Q000235:genetics; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D010179:Pancreas / Q000166:cytology* / Q000196:embryology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013552:Swine; D019098:Telomerase / Q000235:genetics; D014162:Transfection
chemical_list: D002096:C-Peptide; D007328:Insulin; C509186:TERT protein, human; D019098:Telomerase