title: MOHITO, a novel mouse cytokine-dependent T-cell line, enables studies of oncogenic signaling in the T-cell context.
abstract: The mouse pro-B cell line Ba/F3 has gained major interest as a model system to investigate oncogenic tyrosine kinases and to determine the efficacy of kinase inhibitors. While Ba/F3 cells are suitable to study oncogenic kinases derived from various cell types, the signaling networks in Ba/F3 cells are B-cell specific. We have established a mouse CD4+CD8+ double positive T-cell line (named MOHITO, for MOuse Hematopoietic Interleukin-dependent cell line of T-cell Origin) that has many features of human T-cell acute lymphoblastic leukemia (Notch1 and Jak1 mutation, TCR rearrangement) and is dependent on interleukin-7. The MOHITO cell line can be transformed to cytokine independent proliferation by BCR-ABL1 or mutant JAK1. This mouse T-cell line is a novel model system to investigate protein signaling and inhibition in a T-cell specific context and is a valuable tool to study and verify oncogenic capacity of mutations in the kinome and phosphatome in T-cell malignancies.
authors.affiliation: Department of Molecular and Developmental Genetics, VIB, Leuven, Belgium.
authors.forename: Maria
authors.initials: M
authors.lastname: Kleppe
authors.forename: Nicole
authors.initials: N
authors.lastname: Mentens
authors.forename: Thomas
authors.initials: T
authors.lastname: Tousseyn
authors.forename: Iwona
authors.initials: I
authors.lastname: Wlodarska
authors.forename: Jan
authors.initials: J
authors.lastname: Cools
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015851:Interleukin-7 / Q000378:metabolism* / Q000494:pharmacology; D053613:Janus Kinase 1 / Q000235:genetics / Q000378:metabolism; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D002273:Carcinogens; D015851:Interleukin-7; D051881:Receptor, Notch1; D016044:Fusion Proteins, bcr-abl; D053613:Janus Kinase 1