title: [Calcineurin signaling pathway is involved in cardiomyocyte hypertrophy mediated by calcium activators].
abstract: OBJECTIVE
  To investigate the effects of intracellular free calcium ([Ca2+]i) from different resources on calcineurin (CaN) dependent signaling pathway and cardiomyocyte hypertrophy.
  
  
  METHODS
  Angiotensin II (Ang II,10(-7) mol/L), ryanodine (RY, 10(-7) mol/L), inositol (1,4,5)-trisphosphate (IP3, 10(-7) mol/L) were applied to stimulate the [Ca2+]i, CaN activity and protein expression were measured in primary cultured cardiomyocytes from Wistar rat. [Ca2+]i was measured using Fura-2/AM fluorescent technique. The incorporation of 3H-Leucine (3H-Leu) determined the synthesis rates of protein.
  
  
  RESULTS
  Ang II, RY and IP3 increased [Ca2+]i, promoted CaN activity and protein expression, and 3H-Leu incorporation in cultured cardiomyocytes compared with control (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  Cardiomyocyte hypertrophy is associated with the changes of cellular [Ca2+]i, but not with its resources. Indicating that CaN signaling pathway play an important role in the hypertrophy of cardiomyocytes.
authors.affiliation: Cardiology Department, General Hospital of Chengdu Army Command, Chengdu 610083, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: X W
authors.initials: XW
authors.lastname: Zhou
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012433:Ryanodine / Q000494:pharmacology; D015398:Signal Transduction
chemical_list: D000804:Angiotensin II; D012433:Ryanodine; D015544:Inositol 1,4,5-Trisphosphate; D019703:Calcineurin; D002118:Calcium