title: A chimeric peptide of intestinal trefoil factor containing cholesteryl ester transfer protein B cell epitope significantly inhibits atherosclerosis in rabbits after oral administration.
abstract: Vaccination against cholesteryl ester transfer protein (CETP) is proven to be effective for inhibiting atherosclerosis in animal models. In this study, the proteases-resistant intestinal trefoil factor (TFF3) was used as a molecular vehicle to construct chimeric TFF3 (cTFF3) containing CETP B cell epitope and tetanus toxin helper T cell epitope. It was found that cTFF3 still preserved a trefoil structure, and can resist proteases digestion in vitro. After oral immunization with cTFF3, the CETP-specific IgA and IgG could be found in intestine lavage fluid and serum, and the anti-CETP antibodies could inhibit partial CETP activity to increase high-density lipoprotein cholesterol, decrease low-density lipoprotein cholesterol, and inhibit atherosclerosis in animals. Therefore, TFF3 is a potential molecular vehicle for developing oral peptide vaccines. Our research highlights a novel strategy for developing oral peptide vaccines in the future.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Gaofu
authors.initials: G
authors.lastname: Qi
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Shengying
authors.initials: S
authors.lastname: Wang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Xin
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Qingye
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D050197:Atherosclerosis / Q000517:prevention & control*; D053480:Cholesterol Ester Transfer Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D000071164:Trefoil Factor-2
chemical_list: D053480:Cholesterol Ester Transfer Proteins; D000939:Epitopes; D010455:Peptides; D000071164:Trefoil Factor-2