title: Influence of GGCX genotype on warfarin dose requirements in Chinese patients.
abstract: BACKGROUND
  It has been widely accepted that genetic factors were the major sources of the variation in warfarin dose. This study is intended to investigate whether the 3261G>A variation in GGCX gene influences stable warfarin dose in Chinese patient population.
  
  
  METHODS
  A total of 217 patients with stable warfarin dose were enrolled. Genomic DNA was extracted from each subject and the genotype of GGCX 3261G>A was determined by using of denaturing high-performance liquid chromatography (DHPLC). Least significant difference tests (LSDs) were used to compare dose with genotypes. Analysis of variance (ANVOA) was used to calculate the proportion of warfarin dose that could be explained by variation in genotype.
  
  
  RESULTS
  In the total of 217 subjects, 84 patients (38.7%) were GG homozygote, whereas 117 (53.9%) were GA heterozygote and 16 (7.4%) were AA homozygote. Patients with the GGCX 3261AA genotype had a significantly higher average daily maintenance dose (3.39 ± 1.40 mg) than those with the GG genotype (2.69 ± 1.07 mg; P=0.027), and GGCX 3261G>A explains 2.3% of the univariate warfarin dose variance.
  
  
  CONCLUSIONS
  GGCX 3261G>A may affect warfarin dose requirements, and showed a small but significant effect on warfarin dose in a Chinese patient population.
authors.affiliation: Department of Laboratory, Guizhou Provincial People's Hospital, No.83 East Zhongshan Road, Guiyang 550002, Guizhou, PR China. hsw713@sina.com
authors.forename: Sheng-Wen
authors.initials: SW
authors.lastname: Huang
authors.forename: Dao-Kang
authors.initials: DK
authors.lastname: Xiang
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Bao-Lin
authors.initials: BL
authors.lastname: Chen
authors.forename: Bang-Quan
authors.initials: BQ
authors.lastname: An
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Li
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Luo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000008:administration & dosage*; D044466:Asian People / Q000235:genetics*; D019735:Carbon-Carbon Ligases / Q000235:genetics*; D002681:China; D004247:DNA / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014859:Warfarin / Q000008:administration & dosage*; D055815:Young Adult
chemical_list: D000925:Anticoagulants; D014859:Warfarin; D004247:DNA; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase