title: Preliminary approach of real-time monitoring in vitro matrix mineralization based on surface plasmon resonance detection.
abstract: Matrix mineralization is a terminal process in osteoblast differentiation, and several approaches have been introduced to characterize the process in tissues or cultured cells. However, an analytical technique that quantitates in vitro matrix mineralization of live cells without any labeling or complex treatments is still lacking. In this study, we investigate a simple and enhanced optical method based on surface plasmon resonance (SPR) detection that can monitor the surface-limited refractive index change in real-time. During monitoring MC3T3-E1 cells in vitro culture every 2 days for over 4 weeks, the SPR angle is shifted with a greater resonance change in cells cultured with osteogenic reagents than those without the reagents. In addition, the SPR results obtained have a close relevance with the tendency of conventional mineralization staining and an inductively coupled plasma-based calcium content measure. These results suggest a new approach of a real-time SPR monitoring in vitro matrix mineralization of cultured cells.
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-742, Korea.
authors.forename: Shin Ae
authors.initials: SA
authors.lastname: Kim
authors.forename: Sumit
authors.initials: S
authors.lastname: Das
authors.forename: Howon
authors.initials: H
authors.lastname: Lee
authors.forename: Junghoon
authors.initials: J
authors.lastname: Kim
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Song
authors.forename: In Sook
authors.initials: IS
authors.lastname: Kim
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Byun
authors.forename: Soon Jung
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Sung June
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002113:Calcification, Physiologic; D002454:Cell Differentiation; D004867:Equipment Design; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D012031:Refractometry / Q000295:instrumentation / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*