title: [Activation of PACAP receptor could protect cultured hippocampal neurons against beta amyloid peptide induced neurotoxicity].
abstract: OBJECTIVE
  To observe the protective role of pituitary adenylate cyclase activating polypeptide (PACAP) on hippocampal neuronal apoptosis induced by beta amyloid peptide in the culture.
  
  
  METHODS
  Hippocampal neurons were isolated from 1d old SD rat and neuronal survival and apoptosis were measured by MTT assay and DNA ladder.
  
  
  RESULTS
  25 micromol/L Abeta could induce neuron apoptosis while co-treatment with PACAP could increase the survival of hippocampal neurons. The antagonist of PACAP receptor, P6-27, could reverse the effect of PACAP.
  
  
  CONCLUSIONS
  PACAP could protects cultured neurons from the neurotoxicity of Abeta through the activation of PACAP receptor and may have a bright use in treatment of neurodegenerative disease.
authors.affiliation: Department of Microbiology, Beijing Military Medical College, Beijing 100071, China.
authors.forename: Lan-Run
authors.initials: LR
authors.lastname: Gui
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
authors.forename: Bing-Lie
authors.initials: BL
authors.lastname: Zhang
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide