title: Effect of aluminium metal on glutathione (GSH) level in plasma and cytosolic fraction of human blood.
abstract: Aluminium is being used in the medicines in the form of antacids. The Aluminium metal can be leached from our utensils and can harm the body for its side effects, if become available to the systemic circulation. So it is important to check the effect of Aluminum on the Glutathione in vivo condition. Ellman method was used to determine the effect of Aluminum on GSH level in whole blood spectrophotometerically. 5,5-Dithiobis, 2-Nitrobenzoic Acid, Glutathione, Aluminium sulphate, phosphate buffer, HCl (Hydrochloric acid) and other laboratory instruments were used to conduct the research work. Time dependent effect of Aluminum on Glutathione level in whole blood was also checked and decrease was observed. This study also shows the effect of Aluminum as helping agent for the Glutathione to enhance the antioxidant system of the body or a cause for depletion of reduced Glutathione.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gomal University, D.I. Khan, Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: M Farid
authors.initials: MF
authors.lastname: Khan
authors.forename: Syed Umer
authors.initials: SU
authors.lastname: Jan
authors.forename: Naseem
authors.initials: N
authors.lastname: Ullah
mesh_terms: D000534:Alum Compounds / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D002021:Buffers; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D005978:Glutathione / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013439:Sulfhydryl Reagents / Q000737:chemistry
chemical_list: D000534:Alum Compounds; D000975:Antioxidants; D002021:Buffers; D012996:Solutions; D013439:Sulfhydryl Reagents; C041524:aluminum sulfate; D004228:Dithionitrobenzoic Acid; D005978:Glutathione