title: Oleosin di-or tri-meric fusions with GFP undergo correct targeting and provide advantages for recombinant protein production.
abstract: Plant oleosins are small proteins embedded within the phospholipid monolayer separating the triacylglycerol storage site of embryo-located oilbodies from the cytoplasm of oilseed cells. The potential of oleosins to act as carriers for recombinant proteins foreign to plant cells has been well established. Using this approach, the recombinant polypeptide is accumulated in oilbodies as a fusion with oleosin. DNA constructs having tandemly arranged oleosins followed by GFP or flanked by oleosins were used to transform Arabidopsis plants. In all cases the green fluorescence revealed that the fusion polypeptide had a native conformation and the recombinant proteins were correctly targeted to seed oilbodies. Mobilization of lipids was not retarded when using homo-dimer or -trimer oleosin fusions, since seed production, germination rates and seedling establishment were similar among all constructs, and comparable to wild-type Arabidopsis plants. Plant physiology and growth of recombinant lines were similar to wild-type plants. The construct specifying two oleosins flanking the GFP polypeptide revealed interesting properties regarding both the accumulation and the relative stability of the oilbody protein assembly. Although expression levels varied among transgenic lines, those transgenes accumulated significantly higher levels of fusion proteins as compared to previously reported values obtained by a single-oleosin configuration, reaching up to 2.3% of the total embryo proteins. These results shows that the expression cassettes comprising three oleosin molecules in frame to the GFP molecule or two oleosins flanking the GFP could be advantageous over the single-oleosin configuration for higher production and better commercialization of this plant biotechnological platform without jeopardizing plant vigour and physiology or oilbody stability.
authors.affiliation: Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Banilas
authors.forename: Gerasimos
authors.initials: G
authors.lastname: Daras
authors.forename: Stamatis
authors.initials: S
authors.lastname: Rigas
authors.forename: Maurice M
authors.initials: MM
authors.lastname: Moloney
authors.forename: Polydefkis
authors.initials: P
authors.lastname: Hatzopoulos
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C079353:oleosin protein, Arabidopsis; D049452:Green Fluorescent Proteins