title: Variation of NDRG2 and c-Myc expression in rat heart during the acute stage of ischemia/reperfusion injury.
abstract: N-Myc downstream regulated gene 2 (NDRG2), a Myc-repressed gene, is highly expressed in heart tissue. NDRG2 increases in response to hypoxia-induced stress and is involved in hypoxia-induced radioresistance. However, little is known about the expression changes and possible roles of NDRG2 in the heart under hypoxia condition. Here, the authors show that NDRG2, mainly localized in cardiomyocyte cytoplasm, was significantly reduced in myocardial tissue after acute ischemia/reperfusion (I/R) injury. Meanwhile, c-Myc was up-regulated following acute I/R injury, and the expression of c-Myc was significantly inversely correlated with that of NDRG2. In addition, overexpression of c-Myc in primary cultured cardiomyocyte repressed NDRG2 expression. Furthermore, the increase of cardiomyocyte apoptosis was correlated with the decrease of NDRG2 protein during the acute phase of reperfusion. These data suggested for the first time that I/R injury-induced up-regulation of pro-apoptotic c-Myc expression may contribute to the down-regulation of anti-apoptotic NDRG2. This stress response might be involved in the novel mechanism of myocardial apoptosis induced by I/R injury in rat.
authors.affiliation: Department of Cardiovascular medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Zhongchan
authors.initials: Z
authors.lastname: Sun
authors.forename: Lan
authors.initials: L
authors.lastname: Shen
authors.forename: Xiang
authors.initials: X
authors.lastname: Sun
authors.forename: Guang
authors.initials: G
authors.lastname: Tong
authors.forename: Dongdong
authors.initials: D
authors.lastname: Sun
authors.forename: Tenglong
authors.initials: T
authors.lastname: Han
authors.forename: Guodong
authors.initials: G
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Cao
authors.forename: Libo
authors.initials: L
authors.lastname: Yao
authors.forename: Haichang
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D016259:Genes, myc / Q000235:genetics / Q000502:physiology*; D014644:Genetic Variation; D007150:Immunohistochemistry; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D032383:Myocytes, Cardiac / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C507213:Ndrg2 protein, rat; D009419:Nerve Tissue Proteins