title: Distinct roles of JNK-1 and ERK-2 isoforms in permeability barrier repair and wound healing.
abstract: c-Jun N-terminal kinases (JNKs, also called stress activated protein kinases) and the extra-cellular signal responsive kinases (ERKs) exert different functions in mitogenesis, maturation and differentiation of immune and epithelial cells. We investigated specific functions of individual JNK and ERK isoforms in skin permeability barrier repair and in wound healing. JNK1, but not JNK2 or JNK3, deficient mice revealed a delay in the permeability barrier repair after superficial injury to the skin (tape-stripping) as well as a delay in the healing of full skin thickness wounds. Skin barrier injury induced an increase in epidermal JNK1 enzyme activity in mouse skin in vivo, and JNK1 activity correlated with the degree of differentiation in organotypic keratinocyte cultures. Skin injury activated epidermal ERK2 enzyme activity with biphasic maxima after 30 min and 3h, and the activity was independent from the differentiation state in keratinocyte culture. In summary, superficial and deep wound healing depends on the differential activity of MAP kinases such as JNK1 in epidermal differentiation and ERK2 in proliferation.
authors.affiliation: Department of Dermatology, University of Kiel, Schittenhelmstr. 7, 24105 Kiel, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Koehler
authors.forename: Kirsten
authors.initials: K
authors.lastname: Mielke
authors.forename: Michael
authors.initials: M
authors.lastname: Schunck
authors.forename: Claudia
authors.initials: C
authors.lastname: Neumann
authors.forename: Thomas
authors.initials: T
authors.lastname: Herdegen
authors.forename: Ehrhardt
authors.initials: E
authors.lastname: Proksch
mesh_terms: D000818:Animals; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048055:Mitogen-Activated Protein Kinase 8 / Q000378:metabolism*; D010539:Permeability; D012867:Skin / Q000201:enzymology* / Q000293:injuries*; D014945:Wound Healing / Q000502:physiology*
chemical_list: C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048055:Mitogen-Activated Protein Kinase 8