title: IL-17 production by γδ T cells is important for the antitumor effect of Mycobacterium bovis bacillus Calmette-Guérin treatment against bladder cancer.
abstract: Intravesical inoculation of Mycobacterium bovis bacillus Calmette-Guérin (BCG) has been used for the treatment of bladder cancer. Recent studies implied the requirement of neutrophil infiltration for the antitumor effect. In this study, we found that IL-17 was produced in the bladder after BCG treatment, preceding the infiltration of neutrophils. Neutrophils in the bladder after BCG treatment were reduced in IL-17-deficient mice, in which BCG-induced antitumor effect against intravesically inoculated bladder cancer was abolished. Notably, the level of IL-17 production and the number of neutrophils in BCG-treated bladder was reduced in γδ T-cell-deficient mice but not in CD4-depleted mice. Survival of bladder cancer-inoculated γδ T-cell-deficient mice was not improved by BCG treatment. These results suggest that IL-17-producing γδ T cells play a key role in the BCG-induced recruitment of neutrophils to the bladder, which is essential for the antitumor activity against bladder cancer.
authors.affiliation: Division of Host Defense, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
authors.forename: Ario
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Dejima
authors.forename: Hisakata
authors.initials: H
authors.lastname: Yamada
authors.forename: Kensuke
authors.initials: K
authors.lastname: Shibata
authors.forename: Risa
authors.initials: R
authors.lastname: Nakamura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Eto
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakatani
authors.forename: Seiji
authors.initials: S
authors.lastname: Naito
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Yoshikai
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000276:immunology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D007167:Immunotherapy / Q000379:methods*; D020381:Interleukin-17 / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009163:Mycobacterium bovis / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D001749:Urinary Bladder Neoplasms / Q000628:therapy*
chemical_list: D015704:CD4 Antigens; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta