title: A highly selective and sensitive fluorescence sensing system for distinction between diphosphate and nucleoside triphosphates.
abstract: Among the numerous chemosensors available for diphosphate (P(2)O(7)(4-), PPi) and nucleoside triphosphates (NTPs), only a few can distinguish between PPi and NTPs. Hence, very few bioanalytical applications based on such selective chemosensors have been realized. We have developed a new fluorescence sensing system for distinction between PPi and NTPs based on the combination of two sensors, a binuclear Zn(II) complex (1·2Zn) and boronic acid (BA), in which one chemosensor (1·2Zn) shows signal changes depending on the PPi (or NTP) concentration, and the other (BA) blocks the signal change caused by NTPs; this system enables the distinction of PPi from NTPs and is sensitive to nanomolar concentrations of PPi. The new sensing system has been successfully used for the direct quantification of RNA polymerase activity.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Jae Han
authors.initials: JH
authors.lastname: Lee
authors.forename: A Reum
authors.initials: AR
authors.lastname: Jeong
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Jung
authors.forename: Chung-Mo
authors.initials: CM
authors.lastname: Park
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry*; D015374:Biosensing Techniques; D011756:Diphosphates / Q000032:analysis* / Q000737:chemistry*; D005453:Fluorescence; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009705:Nucleosides / Q000032:analysis* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D011122:Polyphosphates / Q000032:analysis* / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D011756:Diphosphates; D009705:Nucleosides; D009942:Organometallic Compounds; D011122:Polyphosphates; D000255:Adenosine Triphosphate; D015032:Zinc; C005692:triphosphoric acid