title: ZNF224: Structure and role of a multifunctional KRAB-ZFP protein.
abstract: The Kruppel-like zinc finger protein ZNF224 was originally identified as the transcriptional repressor of the human aldolase A gene. ZNF224 transcriptional repression depends on interaction with the corepressor KAP-1 and the recruitment of enzyme activities modifying chromatin, in accordance with repression mechanism of KRAB-ZFP family. Recently, the arginine methyltransferase PRMT5 was demonstrated to play a crucial role in the transcriptional ZNF224 repressor complex. An alternatively spliced isoform, ZNF255, arises from the ZNF224 gene. ZNF224 and ZNF255 have a distinct pattern of distribution within the cell and display a specific pattern of interaction with different molecular partners. These isoform-specific interactions seem to control different cellular pathways. These findings suggest that ZNF224 is a multifunctional protein and that alternative splicing, sub-cellular compartmentalization and isoform-specific interactions may modulate its activity.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy. lupo@unisannio.it
authors.forename: Angelo
authors.initials: A
authors.lastname: Lupo
authors.forename: Elena
authors.initials: E
authors.lastname: Cesaro
authors.forename: Giorgia
authors.initials: G
authors.lastname: Montano
authors.forename: Paola
authors.initials: P
authors.lastname: Izzo
authors.forename: Paola
authors.initials: P
authors.lastname: Costanzo
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics; D000818:Animals; D005786:Gene Expression Regulation; D006801:Humans; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D012097:Repressor Proteins; C470003:ZNF224 protein, human