title: Genetic screens using the piggyBac transposon.
abstract: Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK.
authors.forename: Su Kit
authors.initials: SK
authors.lastname: Chew
authors.forename: Roland
authors.initials: R
authors.lastname: Rad
authors.forename: P Andrew
authors.initials: PA
authors.lastname: Futreal
authors.forename: Allan
authors.initials: A
authors.lastname: Bradley
authors.forename: Pentao
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D004252:DNA Mutational Analysis / Q000379:methods*; D004251:DNA Transposable Elements; D053595:Embryonic Stem Cells; D040461:Gene Components; D052138:Genes, Neoplasm; D005822:Genetic Vectors; D006801:Humans; D006824:Hybridization, Genetic; D053208:Kaplan-Meier Estimate; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D014162:Transfection / Q000379:methods; D019895:Transposases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases; C494471:sleeping beauty transposase, human