title: Bacteria-based AND logic gate: a decision-making and self-powered biosensor.
abstract: We developed a bacteria-based AND logic gate using a Pseudomonas aeruginosa lasI/rhlI double mutant with two quorum-sensing signaling molecules as the input signals. We showed a distinct electrical output signal, despite the complexity and continuous regulation of metabolic reactions of living cells.
authors.affiliation: Department of Biological and Environmental Engineering, Cornell University, 214 Riley-Robb Hall, Ithaca, NY 14853, USA.
authors.forename: Zhongjian
authors.initials: Z
authors.lastname: Li
authors.forename: Miriam A
authors.initials: MA
authors.lastname: Rosenbaum
authors.forename: Arvind
authors.initials: A
authors.lastname: Venkataraman
authors.forename: Tsz Kin
authors.initials: TK
authors.lastname: Tam
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
authors.forename: Largus T
authors.initials: LT
authors.lastname: Angenent
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D008025:Ligases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010619:Phenazines / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D053038:Quorum Sensing / Q000502:physiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C081237:LasI protein, Pseudomonas aeruginosa; D010619:Phenazines; D014157:Transcription Factors; C088386:homoserine lactone; D008025:Ligases; C096955:RHLI protein, Pseudomonas aeruginosa; D015107:4-Butyrolactone