title: Poliovirus 2A(pro) induces the nucleic translocation of poliovirus 3CD and 3C' proteins.
abstract: Poliovirus genomic RNA replication, protein translation, and virion assembly are performed in the cytoplasm of host cells. However, this does not mean that there is no relationship between poliovirus infection and the cellular nucleus. In this study, recombinant fluorescence-tagged poliovirus 3CD and 3C' proteins were shown to be expressed mainly in the cytoplasm of Vero cells in the absence of other viral proteins. However, upon poliovirus infection, many of these proteins redistributed to the nucleus, as well as to the cytoplasm. A series of transfection experiments revealed that the poliovirus 2A(pro) was responsible for the same redistribution of 3CD and 3C' proteins to the nucleus. Furthermore, a mutant 2A(pro) protein lacking protease activity abrogated this effect. The poliovirus 2A(pro) protein was also found to co-localize with the Nup153 protein, a component of the nuclear pore complexes on the nuclear envelope. These data provide further evidence that there are intrinsic interactions between poliovirus proteins and the cell nucleus, despite that many processes in the poliovirus replication cycle occur in the cytoplasm.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, China.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Tian
authors.forename: Zongqiang
authors.initials: Z
authors.lastname: Cui
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hongping
authors.initials: H
authors.lastname: Wei
authors.forename: XianEn
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000086042:3C Viral Proteases; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003546:Cysteine Endopeptidases / Q000378:metabolism* / Q000502:physiology*; D017955:Poliovirus / Q000235:genetics; D021381:Protein Transport; D014162:Transfection; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism* / Q000502:physiology; D014779:Virus Replication / Q000235:genetics
chemical_list: D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases; C057400:picornain 2A, Picornavirus