title: Comparison of viral glycosylation using lectin blotting with Vero cell-derived and mouse brain-derived Japanese encephalitis vaccines.
abstract: We produced a Vero cell-derived inactivated Japanese encephalitis vaccine using a serum-free medium, as a substitute for the conventional mouse brain-derived Japanese encephalitis vaccine. The immunogenicity of this cell-derived vaccine was higher than that of the conventional mouse brain-derived vaccine. The results of a clinical study in humans also demonstrated higher immunogenicity of this cell-derived vaccine. No gene mutation was found in the viral structural proteins derived from Vero cells and mouse brain. So, we conducted a lectin blot analysis, assuming differing glycosylation as a cause of the higher immunogenicity in humans. The results demonstrated that vaccine reactivity varied with lectins, particularly with WGA, DBA, MAM, SSA, SBA, and GS-II. Thus, glycosylation differed with the vaccines, suggesting a possible cause of the differing immunogenicity between mice and humans.
authors.affiliation: Department of Research and Development, Research Center for Biologicals, The Kitasato Institute, 6-111 Arai, Kitamoto-shi, Saitama 364-0026, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Toriniwa
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Komiya
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology; D002522:Chlorocebus aethiops; D016895:Culture Media, Serum-Free; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D006031:Glycosylation; D022321:Japanese Encephalitis Vaccines / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D037102:Lectins / Q000378:metabolism; D051379:Mice; D015164:Vaccines, Inactivated / Q000737:chemistry / Q000276:immunology; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
chemical_list: D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D022321:Japanese Encephalitis Vaccines; D037102:Lectins; D015164:Vaccines, Inactivated; D014764:Viral Proteins