title: Detection of human parechovirus in stool samples collected from children with acute gastroenteritis in Japan during 2007-2008.
abstract: Of 477 stool specimens, which had been screened for rotavirus, adenovirus, norovirus, sapovirus and astrovirus, collected from infants and children with acute gastroenteritis in pediatric clinics encompassing five localities (Sapporo, Tokyo, Maizuru, Osaka, and Saga) in Japan from July 2007 to June 2008, 247 negative samples (51.7%) were subjected to screening for human parechovirus. Human parechovirus (HPeV) was detected by RT-PCR using a primer pair to amplify 5'UTR region of its genome and was genotyped by sequencing of the VP1 gene. HPeV was detected in 20 of 247 specimens tested, and the detection rate was found to be 8.1%. Seventeen of the 20 strains that tested positive for HPeV were sequenced successfully the VP1 gene. The majority of the HPeV strains (n = 15) could be identified as HPeV1, and the remaining 2 strains could be typed as HPeV3. By phylogenetic and identical matrix analyses of HPeV VP1 sequences, HPeV1 should be divided into two lineages, and all of the Japanese studied HPeV1 strains belong to the lineage 2 accordingly. This is the first report of the circulation of HPeV, especially HPeV1 in Japan.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Ngan Thi Kim
authors.initials: NT
authors.lastname: Pham
authors.forename: Wisoot
authors.initials: W
authors.lastname: Chan-It
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nishimura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kikuta
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sugita
authors.forename: Tsuneyoshi
authors.initials: T
authors.lastname: Baba
authors.forename: Atsuko
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000208:Acute Disease; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000175:diagnosis* / Q000821:virology; D020815:Polyproteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; D020815:Polyproteins; D014764:Viral Proteins