title: Coomassie staining as loading control in Western blot analysis.
abstract: In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
authors.affiliation: Department of Oncology, Clinical Sciences, Lund University, Sweden.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Welinder
authors.forename: Lars
authors.initials: L
authors.lastname: Ekblad
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D010449:Peptide Mapping / Q000379:methods; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue