title: [Non-obstructive azoospermia: option of the testicular sperm extraction performed on the day of oocyte retrieval].
abstract: OBJECTIVE
  Analyzing the results and validating the procedure of testicular sperm extraction (TESE) performed on the day of oocyte retrieval in non obstructive azoospermia (NOA) patients.
  
  
  METHODS
  Sixty TESE were performed on the day of oocyte retrieval (dOR), in 52 NOA men. Patients were sorted into three groups according to the results of the surgical procedure: 1: sperm recovery with possible sperm freezing (n=20); 2: sperm recovery without freezing (n=27); 3: "negative" biopsy (n=13). ICSI outcomes in the two groups with sperm recovery were compared to those of ICSI performed with frozen-thawed sperm obtained from TESE performed (n=13).
  
  
  RESULTS
  The rate of positive sperm retrieval was 78%. While the overall clinical pregnancy rate was 50%, no difference in the fertilization, implantation and clinical pregnancy rates was found in the two groups with positive sperm retrieval as compared to frozen-thawed sperm group. Twelve pregnancies were obtained in patients without further sperm cryopreservation.
  
  
  CONCLUSIONS
  After TESE in NOA men, cryopreserved sperm produced comparable results with freshly obtained sperm. However, TESE performed on dOR can offer the opportunity, in patients with rare sperm that might not survive freeze-thaw, to have a possible fresh embryo transfer. Couples should be counselled regarding the possibility of oocyte retrieval without sperm for ICSI.
authors.affiliation: Service de gynécologie-obstétrique et médecine de la reproduction, hôpital Antoine-Béclère, AP-HP, 157, rue de la Porte-de-Trivaux, 92141 Clamart, France. michael.grynberg@abc.aphp.fr
authors.forename: M
authors.initials: M
authors.lastname: Grynberg
authors.forename: N
authors.initials: N
authors.lastname: Chevalier
authors.forename: A
authors.initials: A
authors.lastname: Mesner
authors.forename: L
authors.initials: L
authors.lastname: Rocher
authors.forename: N
authors.initials: N
authors.lastname: Prisant
authors.forename: S
authors.initials: S
authors.lastname: Madoux
authors.forename: E
authors.initials: E
authors.lastname: Feyereisen
authors.forename: S
authors.initials: S
authors.lastname: Ferlicot
authors.forename: R
authors.initials: R
authors.lastname: Fanchin
authors.forename: R
authors.initials: R
authors.lastname: Frydman
authors.forename: N
authors.initials: N
authors.lastname: Frydman
authors.forename: V
authors.initials: V
authors.lastname: Izard
mesh_terms: D053713:Azoospermia / Q000150:complications* / Q000235:genetics; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000628:therapy*; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D013737:Testis / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods*