title: Varicella-zoster virus transcriptome in latently infected human ganglia.
abstract: We recently developed a novel multiplex reverse transcription (RT)-PCR assay that allows rapid and sensitive detection of transcripts corresponding to all 68 unique varicella-zoster virus (VZV) open reading frames (ORFs) in only five amplification reactions (M. A. Nagel, D. Gilden, T. Shade, B. Gao, and R. J. Cohrs, J. Virol. Methods 157:62-68, 2009). Herein, we applied multiplex RT-PCR analysis to mRNA extracted from 26 trigeminal ganglia latently infected with VZV and one control trigeminal ganglion negative for VZV DNA that were removed from 14 men and women, 16 to 84 years of age, within 24 h after death. Analysis identified VZV transcripts mapping to VZV ORFs 29, 62, and 63, previously detected and sequence verified; VZV ORFs 4 and 40, previously detected by in situ hybridization; and VZV ORFs 11, 41, 43, 57, and 68, not previously detected. VZV ORF 63 transcripts were the most prevalent. Comparison of the 10 VZV ORFs transcribed during latency to their herpes simplex virus type 1 homologues reveals that the latently transcribed VZV genes encode immediate-early, early, and late transcripts.
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Nagel
authors.forename: Alexander
authors.initials: A
authors.lastname: Choe
authors.forename: Igor
authors.initials: I
authors.lastname: Traktinskiy
authors.forename: Robert
authors.initials: R
authors.lastname: Cordery-Cotter
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D005260:Female; D005724:Ganglia / Q000821:virology*; D020869:Gene Expression Profiling; D006562:Herpes Zoster / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics; D017735:Virus Latency; D055815:Young Adult
chemical_list: D014764:Viral Proteins