title: Use of a novel micro-fluidic device to create arrays for multiplex analysis of large and small molecular weight compounds by surface plasmon resonance.
abstract: There is an increasing demand to develop biosensor monitoring devices capable of biomarker profiling for predicting animal adulteration and detecting multiple chemical contaminants or toxins in food produce. Surface plasmon resonance (SPR) biosensors are label free detection systems that monitor the binding of specific biomolecular recognition elements with binding partners. Essential to this technology are the production of biochips where a selected binding partner, antibody, biomarker protein or low molecular weight contaminant, is immobilised. A micro-fluidic immobilisation device allowing the covalent attachment of up to 16 binding partners in a linear array on a single surface has been developed for compatibility with a prototype multiplex SPR analyser. The immobilisation unit and multiplex SPR analyser were respectively evaluated in their ability to be fit-for-purpose for binding partner attachment and detection of high and low molecular weight molecules. The multiplexing capability of the dual technology was assessed using phycotoxin concentration analysis as a model system. The parent compounds of four toxin groups were immobilised within a single chip format and calibration curves were achieved. The chip design and SPR technology allowed the compartmentalisation of the binding interactions for each toxin group offering the added benefit of being able to distinguish between toxin families and perform concentration analysis. This model is particularly contemporary with the current drive to replace biological methods for phycotoxin screening.
authors.affiliation: Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University, Stranmillis Road, Belfast BT9 5AG, UK. katrina.campbell@qub.ac.uk
authors.forename: Katrina
authors.initials: K
authors.lastname: Campbell
authors.forename: Terry
authors.initials: T
authors.lastname: McGrath
authors.forename: Stefan
authors.initials: S
authors.lastname: Sjölander
authors.forename: Thord
authors.initials: T
authors.lastname: Hanson
authors.forename: Mattias
authors.initials: M
authors.lastname: Tidare
authors.forename: Osten
authors.initials: O
authors.lastname: Jansson
authors.forename: Anna
authors.initials: A
authors.lastname: Moberg
authors.forename: Mark
authors.initials: M
authors.lastname: Mooney
authors.forename: Christopher
authors.initials: C
authors.lastname: Elliott
authors.forename: Jos
authors.initials: J
authors.lastname: Buijs
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized; D004867:Equipment Design; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014118:Toxins, Biological / Q000032:analysis / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D055910:Antibodies, Immobilized; D011506:Proteins; D014118:Toxins, Biological; D014874:Water Pollutants, Chemical