title: Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model.
abstract: We report the use of spectral Doppler optical coherence tomography imaging (SDOCTI) for quantitative evaluation of dynamic blood circulation before and after a localized ischemic stroke in a mouse model. Rose Bengal photodynamic therapy (PDT) is used as a noninvasive means for inducing localized ischemia in cortical microvasculature of the mouse. Fast, repeated Doppler optical coherence tomography scans across vessels of interest are performed to record flow dynamic information with high temporal resolution. Doppler-angle-independent flow indices are used to quantify vascular conditions before and after the induced ischemia by the photocoagulation of PDT. The higher (or lower) flow resistive indices are associated with higher (or lower) resistance states that are confirmed by laser speckle flow index maps (of laser speckle imaging). Our in vivo experiments shows that SDOCTI can provide complementary quantified flow information that is an alternative to blood volume measurement, and can be used as a means for cortical microvasculature imaging well suited for small animal studies.
authors.affiliation: University of California, Department of Biomedical Engineering, Irvine, CA 92612-1475, USA. yulingfeng@gmail.com
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Yu
authors.forename: Elaine
authors.initials: E
authors.lastname: Nguyen
authors.forename: Gangjun
authors.initials: G
authors.lastname: Liu
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D002545:Brain Ischemia / Q000150:complications / Q000473:pathology* / Q000503:physiopathology*; D004195:Disease Models, Animal; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation*; D051379:Mice; D008809:Mice, Inbred C3H; D010092:Oximetry / Q000295:instrumentation; D013057:Spectrum Analysis / Q000295:instrumentation*; D020521:Stroke / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*