title: Molecular epidemiological analysis of paired pol/env sequences from Portuguese HIV type 1 patients.
abstract: The advent of new therapeutic approaches targeting env and the search for efficient anti-HIV-1 vaccines make it necessary to identify the number of recombinant forms using genomic regions that were previously not frequently sequenced. In this study, we have subtyped paired pol and env sequences from HIV-1 strains infecting 152 patients being clinically followed in Portugal. The percentage of strains in which we found discordant subtypes in pol and env was 25.7%. When the subtype in pol and env was concordant (65.1%), the most prevalent subtypes were subtype B (40.8%), followed by subtype C (17.8%) and subtype G (5.3%). The most prevalent recombinant form was CRF14_BGpol/Genv (7.2%).
authors.affiliation: Centro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Lisbon, Portugal. ana.abecasis@gmail.com
authors.forename: Ana B
authors.initials: AB
authors.lastname: Abecasis
authors.forename: Andreia
authors.initials: A
authors.lastname: Martins
authors.forename: Inês
authors.initials: I
authors.lastname: Costa
authors.forename: Ana P
authors.initials: AP
authors.lastname: Carvalho
authors.forename: Isabel
authors.initials: I
authors.lastname: Diogo
authors.forename: Perpétua
authors.initials: P
authors.lastname: Gomes
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Camacho
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D011174:Portugal / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus