title: MOS11: a new component in the mRNA export pathway.
abstract: Nucleocytoplasmic trafficking is emerging as an important aspect of plant immunity. The three related pathways affecting plant immunity include Nuclear Localization Signal (NLS)-mediated nuclear protein import, Nuclear Export Signal (NES)-dependent nuclear protein export, and mRNA export relying on MOS3, a nucleoporin belonging to the Nup107-160 complex. Here we report the characterization, identification, and detailed analysis of Arabidopsis modifier of snc1, 11 (mos11). Mutations in MOS11 can partially suppress the dwarfism and enhanced disease resistance phenotypes of snc1, which carries a gain-of-function mutation in a TIR-NB-LRR type Resistance gene. MOS11 encodes a conserved eukaryotic protein with homology to the human RNA binding protein CIP29. Further functional analysis shows that MOS11 localizes to the nucleus and that the mos11 mutants accumulate more poly(A) mRNAs in the nucleus, likely resulting from reduced mRNA export activity. Epistasis analysis between mos3-1 and mos11-1 revealed that MOS11 probably functions in the same mRNA export pathway as MOS3, in a partially overlapping fashion, before the mRNA molecules pass through the nuclear pores. Taken together, MOS11 is identified as a new protein contributing to the transfer of mature mRNA from the nucleus to the cytosol.
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, Canada.
authors.forename: Hugo
authors.initials: H
authors.lastname: Germain
authors.forename: Na
authors.initials: N
authors.lastname: Qu
authors.forename: Yu Ti
authors.initials: YT
authors.lastname: Cheng
authors.forename: Eunkyoung
authors.initials: E
authors.lastname: Lee
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Oliver Xiaoou
authors.initials: OX
authors.lastname: Dong
authors.forename: Patrick
authors.initials: P
authors.lastname: Gannon
authors.forename: Shuai
authors.initials: S
authors.lastname: Huang
authors.forename: Pingtao
authors.initials: P
authors.lastname: Ding
authors.forename: Yingzhong
authors.initials: Y
authors.lastname: Li
authors.forename: Fred
authors.initials: F
authors.lastname: Sack
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D021581:Active Transport, Cell Nucleus; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C556905:MOS11 protein, Arabidopsis; C507155:MOS3 protein, Arabidopsis; D028861:Nuclear Pore Complex Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C482739:SNC1 protein, Arabidopsis