title: Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress.
abstract: The intestinal epithelium contains columnar epithelial cells (ECs) and M cells, and fucosylation of the apical surface of ECs and M cells is involved in distinguishing the two populations and in their response to commensal flora and environmental stress. Here, we show that fucosylated ECs (F-ECs) were induced in the mouse small intestine by the pro-inflammatory agents dextran sodium sulfate and indomethacin, in addition to an enteropathogen derived cholera toxin. Although F-ECs showed specificity for the M cell-markers, lectin Ulex europaeus agglutinin-1 and our monoclonal antibody NKM 16-2-4, these cells also retained EC-phenotypes including an affinity for the EC-marker lectin wheat germ agglutinin. Interestingly, fucosylation of Peyer's patch M cells and F-ECs was distinctly regulated by α(1,2)fucosyltransferase Fut1 and Fut2, respectively. These results indicate that Fut2-mediated F-ECs share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells.
authors.affiliation: Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Terahara
authors.forename: Tomonori
authors.initials: T
authors.lastname: Nochi
authors.forename: Masato
authors.initials: M
authors.lastname: Yoshida
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Hatai
authors.forename: Shiho
authors.initials: S
authors.lastname: Kurokawa
authors.forename: Myoung Ho
authors.initials: MH
authors.lastname: Jang
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kweon
authors.forename: Steven E
authors.initials: SE
authors.lastname: Domino
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Yuki
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Tsunetsugu-Yokota
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D005643:Fucose / Q000378:metabolism*; D005647:Fucosyltransferases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology*; D007421:Intestine, Small / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D037121:Plant Lectins / Q000276:immunology; D013312:Stress, Physiological / Q000276:immunology*; D014158:Transcription, Genetic; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005643:Fucose; D005647:Fucosyltransferases; D037121:Plant Lectins; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C036850:Ulex europaeus lectins