title: Botanical micelle and its application for direct electrochemical biosensor.
abstract: This paper is concerned about the entrapment of horseradish peroxidase (HRP) within botanical inositol hexakisphosphoric (IP(6)) micelles for the preparation of enzyme biosensor. The good affinity of IP(6) micelles with the enzyme provides naturally biocompatible microenvironment for the enzyme immobilization, achieving the direct electron transfer between HRP and electrode surface. The resulting biosensor to H(2)O(2) detection exhibits a low detection limit of 0.1 μmol L(-1) (S/N = 3), a quick response time (3s), and a long-term stability. The apparent Michaelis-Menten constant is quite tiny about 0.0016 mmol L(-1).
authors.affiliation: Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Miao
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.forename: Weixun
authors.initials: W
authors.lastname: Zhou
authors.forename: Zongrang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Yang
mesh_terms: D001672:Biocompatible Materials; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004579:Electron Transport; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D010833:Phytic Acid; D010944:Plants / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D008823:Micelles; D010833:Phytic Acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase