title: [Effect of CoCl2 pretreatment on Na + and K+ currents of the rat hippocampal neurons after acute hypoxia].
abstract: OBJECTIVE
  To study effect of CoCl2 pretreatment on the voltage-gated Na+ and K+ currents of the rat hippocampal neurons after acute hypoxia.
  
  
  METHODS
  Primarily cultured hippocampal neurons were divided into CoCl2 pretreated and non-pretreated groups. Patch clamp whole cell recording technique was used to examine Na+ and K+ currents of the hippocampal neurons.
  
  
  RESULTS
  After acute hypoxia, I(Na) and I(K) of the hippocampal neurons were significantly decreased and the threshold of I(Na) was right-shifted. Pretreatment of the neurons with CoCl2 inhibited the reduction of I(Na) and I(K).
  
  
  CONCLUSIONS
  CcCl2 pretreatment alleviates the acute hypoxia-induced changes of I(Na) and I(K), which may be one of the mechanisms for the protective effect of CoCl2 on neurons.
authors.affiliation: Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Ai-Shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Fu-Zhuang
authors.initials: FZ
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D003035:Cobalt / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology* / Q000503:physiopathology; D009474:Neurons / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D015221:Potassium Channels; D015222:Sodium Channels; D003035:Cobalt; C018021:cobaltous chloride