title: Detection of ER stress in vivo by Raman spectroscopy.
abstract: The endoplasmic reticulum (ER) is an organelle in which most membrane and secretory proteins are synthesized. If these proteins are not folded correctly, unfolded proteins accumulate in the ER lumen, causing a cellular situation known as ER stress. Recently, many studies on the relationship between ER stress and diseases have been reported. Thus, studies of ER stress in vivo should yield information that is useful in pathology. Model mice have been developed as a powerful tool to visualize ER stress in vivo, but this approach depends on transgenic technology. Here, we report on a method of detecting ER stress in vivo by Raman spectroscopy. Our experiments revealed that two specific Raman bands were reduced in both cultured cells and animal tissues in an ER stress dependent manner. This suggests that Raman spectroscopy could be a useful tool to detect ER stress in vivo without transgenic technology.
authors.affiliation: Iwawaki Initiative Research Unit, Advanced Science Institute, RIKEN, Wako, Saitama, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosoda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Maruyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Oikawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oshima
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Komachi
authors.forename: Genichi
authors.initials: G
authors.lastname: Kanai
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sato
authors.forename: Takao
authors.initials: T
authors.lastname: Iwawaki
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D011506:Proteins / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013312:Stress, Physiological; D056811:Unfolded Protein Response
chemical_list: D011506:Proteins