title: [Effects of burn on synthesis and secretion of endothelin-1 in rat paraventricular nucleus of hypothalamus].
abstract: OBJECTIVE
  In order to investigate the pathophysiological role of ET-1 in paraventricular nucleus of hypothalamus (PVH) under burn, changes in synthesis and secretion of endothelin-1 (ET-1) in PVH after burn were observed by in situ hybridization and immunohistochemistry. The intensity of ET-1 mRNA and ET-1-immunoreactivity (ET-1-ir) were quantized by image analysis.
  
  
  RESULTS
  Compared with the control group (sham burn, 100% +/- 25%), no significant change in the intensity of ET-1 mRNA positive hybridization signal in PVH was found at 15 min postburn, while the intensity increased significantly both at 60 min (138% +/- 26%, P < 0.05) and at 180 min postburn (167% +/- 18%, P < 0.01). Intensity of ET-1-ir in the neurons in PVH decreased significantly at 15 min postburn to 6.3% +/- 1.5% of the control (P < 0.01) and gradually recovered to 23.1% +/- 2.9% and to 44.1% +/- 3.8% at 60 min and 180 min postburn respectively, while still significantly lower than that in control (P < 0.01).
  
  
  CONCLUSIONS
  The results demonstrated that the synthesis and secretion of ET-1 in rat PVH increased significantly after burn in rats, suggesting that ET-1 in PVH played an important pathophysiological role under burn.
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Jiang
authors.forename: W J
authors.initials: WJ
authors.lastname: Yuan
authors.forename: Z H
authors.initials: ZH
authors.lastname: Xiang
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: J J
authors.initials: JJ
authors.lastname: Xu
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism*; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019332:Endothelin-1; D012333:RNA, Messenger