title: [Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay].
abstract: OBJECTIVE
  To improve specificity and accuracy of endogenous ouabain measurement assay.
  
  
  METHODS
  Anti-ouabain polyclonal antibody egg yolk (IgY) and anti-ouabain rabbit antibody (IgG) were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay (ELISA) were compared.
  
  
  RESULTS
  The ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%, and the inter-assay CV was 2.34% respectively. In contrast, IgG were 2.83% and 3.29%. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs. 5.95%, 3.20% vs. 5.20% of crossreaction with cedilanid and digoxin.
  
  
  CONCLUSIONS
  The specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.
authors.affiliation: Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ming-juan
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Heng
authors.initials: H
authors.lastname: Ge
authors.forename: Lei
authors.initials: L
authors.lastname: Qiang
authors.forename: Zong-ming
authors.initials: ZM
authors.lastname: Duan
authors.forename: Cong-xia
authors.initials: CX
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Zhuo-rern
authors.initials: ZR
authors.lastname: Lu
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002645:Chickens / Q000276:immunology; D003429:Cross Reactions; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology*; D008297:Male; D010042:Ouabain / Q000032:analysis*; D011817:Rabbits
chemical_list: C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D010042:Ouabain