title: Assessment of mercury(II) bioavailability using a bioluminescent bacterial biosensor: practical and theoretical challenges.
abstract: Critical methodological challenges in the microbial biosensor approach to assessing Hg(II) bioavailability were evaluated from the perspective of analytical chemists. The main challenge stems from the fact that the chemical speciation of Hg(II) in natural waters exerts a major control on its bioavailability, yet its natural complexation equilibria are extensively altered during conventional bioassays. New data, obtained using a bioluminescent Hg(II)-biosensor, that illustrate these challenges are presented and potential solutions proposed.
authors.affiliation: Department of Sanitation and Environmental Health, National School of Public Health, Oswaldo Cruz Foundation. Rio de Janeiro, RJ, 21041-210, Brasil. paulo.barrocas@ensp.fiocruz.br
authors.forename: Paulo R G
authors.initials: PR
authors.lastname: Barrocas
authors.forename: William M
authors.initials: WM
authors.lastname: Landing
authors.forename: Robert J M
authors.initials: RJ
authors.lastname: Hudson
mesh_terms: D001682:Biological Availability; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000378:metabolism / Q000493:pharmacokinetics; D008164:Luminescent Proteins; D008628:Mercury / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D004785:Environmental Pollutants; D008164:Luminescent Proteins; D008628:Mercury