title: Integrity of foot-and-mouth disease virions bound to the cells.
abstract: In this study, we examined the integrity of Foot-and-mouth disease virus (FMDV) particles during their binding to the surface of BHK-21 cells under physiological condition. For monitoring of the virus integrity we used blocking of the endocytosis with dynasore and cytochalasin D followed by RT-PCR for viral protein VP1 and the resistance of FMDV to the treatment of RNase A. Our results showed that integrin binding to VP1 did not cause a substantial conformational change in the viral capsid. Furthermore, treatment with RNase A showed no effect on the infectivity of intact as well as cell-bound virions. Our findings confirmed that FMDV entered the host cells in the form of intact virions.
authors.affiliation: Shangai Academy of Agricultural Sciences, Shangai, People's Republic of China. yijianzhong@yahoo.com
authors.forename: J
authors.initials: J
authors.lastname: Yi
authors.forename: Ch
authors.initials: Ch
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005536:Foot-and-Mouth Disease / Q000378:metabolism / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000502:physiology*; D016023:Integrins / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000502:physiology*; D053585:Virus Attachment
chemical_list: D016023:Integrins; D014764:Viral Proteins