title: Identification of the gene cluster involved in muraymycin biosynthesis from Streptomyces sp. NRRL 30471.
abstract: Muraymycin, a potent translocase I inhibitor with clinical potential, is produced by Streptomyces sp. NRRL 30471. The structure of muraymycin is highly unusual and contains the hexahydro-2-imino-4-pyrimidylglycyl moiety (epicapreomycidine) and an ureido bond. Here we report the identification of the muraymycin gene cluster from Streptomyces sp. NRRL 30471. Sequencing analysis of a 43.4-kb contiguous region revealed 33 ORFs, 26 of which were proposed to be involved in muraymycin biosynthesis. Independent targeted inactivation of mur16 and mur17 directly abolished muraymycin production, demonstrating the role of the genes essential for muraymycin biosynthesis. These data provide insights into the molecular mechanisms for muraymycin biosynthesis, and lay a foundation for the generation of muraymycin derivatives with enhanced bioactivity via the strategies of combinatorial biosynthesis.
authors.affiliation: Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Wenqing
authors.initials: W
authors.lastname: Chen
authors.forename: Lipeng
authors.initials: L
authors.lastname: Zhai
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xu
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.forename: Shuangjun
authors.initials: S
authors.lastname: Lin
authors.forename: Xiufen
authors.initials: X
authors.lastname: Zhou
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000378:metabolism; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D013237:Stereoisomerism; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents