title: [Inhibitory effects of kappa-opioid receptor stimulation on cultured myocardial cells].
abstract: OBJECTIVE
  In order to study the effects of kappa-opioid receptor activation, we used cultured cardiomyocytes to study the inhibitory effects of U50,488H on cellular proliferation, protein content in the presence or absence of nor-binaltorphimine (nor-BNI).
  
  
  METHODS
  The cellular proliferation was determined with crystal violet staining and the protein content was assayed with Lowry's method.
  
  
  RESULTS
  A kappa-opioid receptor agonist U50,488H at 0.1 micromol/L-10 micromol/L inhibited the cellular proliferation and protein content of cultured myocardial cells in a dose-dependent manner. The inhibitory effects of U50,488H were completely blocked by pretreatment with nor-BNI, a specific kappa-opioid receptor antagonist at 1 micromol/L.
  
  
  CONCLUSIONS
  U50,488H inhibited the cultured myocardial cells' growth. The inhibitory effects of U50,488H are involved in mediating the action of kappa-opioid receptor stimulation.
authors.affiliation: Department of Pharmacology, Jinzhou Medical College, Jinzhou 121001, China.
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Yang
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Wang
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Yang
mesh_terms: D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / Q000494:pharmacology*; D000818:Animals; D048708:Cell Growth Processes; D002478:Cells, Cultured; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017473:Receptors, Opioid, kappa / Q000378:metabolism*
chemical_list: D017473:Receptors, Opioid, kappa; D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer