title: [Expression of BCSG1-siRNA in tumor transplants of human breast cancer cell line in nude mice].
abstract: OBJECTIVE
  To investigate the inhibitory effects of siRNA targeting BCSG1 gene expression in tumor transplants of human breast cancer cell line in nude mice.
  
  
  METHODS
  Four-pairs of small interfering RNA sequences of BCSG1 were chemically synthesized and inserted into the plasmid expression vectors, and were then transfected into human breast carcinoma cell line MCF7 by liposome method. Plasmid vector with unrelated sequence was used as the vector control. Cells transfected with 4 siRNA sequences, control vector and naive FCF7 cells were transplanted into the nude mice. The tumor inhibition was analysised. Immunohistochemical SP method and semi-quantitative RT-PCR were adopted to detect the BCSG1 mRNA and protein expression, respectively. Breast tissue samples of human infiltrating ductal carcinoma, ductal hyperplasia and fibroadenoma were also used as the controls.
  
  
  RESULTS
  The inhibition rates of tumor growth in four BCSG1-siRNA transfected groups were remarkably higher than those of the vector control group and naive MCF7 cells (P<0.01). Compared with that of the vector control and naïve MCF7 cell group, there was a significant decrease of BCSG-1 protein expression in the four experimental groups by immuno-histochemistry staining (P<0.01). In addition, BCSG1 mRNA expression in the four groups transfected with BCSG1-siRNA were significantly less than that of the control vector group, naive MCF7 cell control group and human breast IDC (P<0.01).
  
  
  CONCLUSIONS
  BCSG1-siRNA down-regulates the expression of BCSG1 and inhibits effectively growth of the transplaned human breast cancer cell line in nude mice.
authors.affiliation: Department of Pathology, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. shpeihong2008@163.com
authors.forename: Pei-hong
authors.initials: PH
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yun-han
authors.initials: YH
authors.lastname: Zhang
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Zhang
authors.forename: Qing-xia
authors.initials: QX
authors.lastname: Fan
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D018226:Fibroadenoma / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D011897:Random Allocation; D014162:Transfection; D047368:Tumor Burden; D051845:gamma-Synuclein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C104249:SNCG protein, human; D051845:gamma-Synuclein