title: Using isothermal titration calorimetry to real-time monitor the heat of metabolism: a case study using PC12 cells and Aβ(1-40).
abstract: Isothermal titration calorimetry (ITC) is one of the most powerful means for direct determination of thermodynamic information associated with most physiochemical and biological processes. The deposition and aggregation of β-amyloid (Aβ) on cell membranes was considered as one of the primary factors in having Alzheimer's disease (AD). Recently, a growing body of evidence has suggested that plasma membrane could accelerate the accumulation of Aβ on the plasma membranes. However, the mechanism of AD is still a controversial issue. This study provided a biothermodynamic approach to real-time monitor the heat of metabolism involved in the co-incubation of PC12 cells and Aβ(1-40) by ITC. The effects of Aβ conformation and concentration of oligomeric Aβ on cytotoxicity were successfully distinguished by ITC. This approach with rapid and direct measurement may provide not only real-time information for the effects of Aβ species on living cells but also a platform for the screening of drug candidates for AD.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Steven S-S
authors.initials: SS
authors.lastname: Wang
authors.forename: Ming-Shen
authors.initials: MS
authors.lastname: Lin
authors.forename: Shen-Liang
authors.initials: SL
authors.lastname: Chen
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.forename: Rouch-Chyu
authors.initials: RC
authors.lastname: Ruaan
authors.forename: Wen-Yih
authors.initials: WY
authors.lastname: Chen
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002151:Calorimetry; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D016716:PC12 Cells; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011487:Protein Conformation; D051381:Rats; D013997:Time Factors
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)