title: [Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC) guidelines for the diagnosis of invasive fungal infections. 2010 update].
abstract: These guidelines are an update of recommendations for the diagnosis of invasive fungal infections by the Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC) published in 2004 (Enferm Infecc Microbiol Clin. 2004, 22:32-9). In this updated version of the guidelines, a comprehensive review of the most recent diagnostic innovations and levels of evidence to recommend those diagnostic procedures are included. We first analyse conventional diagnostic methods, microscopic examination and culture, underlining their limitations which have led to the development of alternative methods, such as fungal antigen and DNA quantification. Those alternative methods of diagnosis are analysed by fungal infection. We also briefly review the methods for molecular identification of fungal species and recommendations for carrying out susceptibility tests for antifungal drugs, including reference procedures, commercial techniques and their indications.
authors.affiliation: Servicio de Microbiología, Hospital Universitario de Bellvitge, L'Hospitalet de Llobregat, Barcelona, España.
authors.forename: Josefina
authors.initials: J
authors.lastname: Ayats
authors.forename: Estrella
authors.initials: E
authors.lastname: Martín-Mazuelos
authors.forename: Javier
authors.initials: J
authors.lastname: Pemán
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quindós
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez
authors.forename: Julio
authors.initials: J
authors.lastname: García-Rodríguez
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
authors.forename: Jesús
authors.initials: J
authors.lastname: Guinea
authors.forename: María J
authors.initials: MJ
authors.lastname: Linares
authors.forename: José
authors.initials: J
authors.lastname: Pontón
authors.forename: Juan L
authors.initials: JL
authors.lastname: Rodríguez-Tudela
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis; D002177:Candidiasis / Q000175:diagnosis; D006801:Humans; D009172:Mycology / Q000379:methods; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology