title: Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR.
abstract: Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. To establish a sensitive, specific assay for the detection and quantitation of PCV2, we designed and synthesized specific primers and a probe in the open reading frame 2. The assay had a wide dynamic range with excellent linearity and reliable reproducibility, and detected between 102 and 1010 copies of the genomic DNA per reaction. The coefficient of variation for Ct values varied from 0.59% to 1.05% in the same assay and from 1.9% to 4.2% in 10 different assays. The assay did not cross-react with porcine circovirus type 1, porcine reproductive and respiratory, porcine epidemic diarrhea, transmissible gastroenteritis of pigs and rotavirus. The limits of detection and quantitation were 10 and 100 copies, respectively. Using the established real-time PCR system, 39 of the 40 samples we tested were detected as positive.
authors.affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Fangting
authors.initials: F
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.forename: Lianlong
authors.initials: L
authors.lastname: Zhu
authors.forename: Chunhua
authors.initials: C
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhang
authors.forename: Furong
authors.initials: F
authors.lastname: Tan
authors.forename: Jinbin
authors.initials: J
authors.lastname: Wang
authors.forename: Shiru
authors.initials: S
authors.lastname: Tao
authors.forename: Xizhong
authors.initials: X
authors.lastname: He
authors.forename: Zongqing
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xueming
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D017925:Circovirus / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D016133:Polymerase Chain Reaction; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000175:diagnosis* / Q000821:virology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D017931:DNA Primers