title: [Simultaneous determination of ginsenosides and epimedium flavonoids in rat urine by HPLC-UV-ELSD].
abstract: OBJECTIVE
  To develop and validate a HPLC-UV-ELSD method for the simultaneous determination of ginsenosides and epimedium flavonoids in rat urine after intravenous administration of Jiweiling freeze-dried powder.
  
  
  METHODS
  Chromatographic separation was performed on a C18 HPLC column, with gradient elution of acetonitrile and water as mobile phase. An UV detector was used at detection wavelength of 220 nm. An evaporative light scattering detector (ELSD) was used at drift tube temperature of 80 degrees C and gas pressure of 172.4 kPa.
  
  
  RESULTS
  The calibration curves were linear over the investigated concentration ranges with all correlation coefficients higher than 0.998. The a intra- and inter-day RSD were less than 9.1% and the relative errors were verage extraction recoveries for all compounds were between 88.67% and 101.2%. The within the range of -11.58% to 10.89%.
  
  
  CONCLUSIONS
  The proposed method showed appropriate accuracy and selectivity and was successfully applied to the rat urine samples analysis of saponins and flavonoids after intravenous administration of Jiweiling freeze-dried powder, which may provide some references to the apprehension of the action mechanism and clinical application.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China. liuminyan75@163.com
authors.forename: Minyan
authors.initials: M
authors.lastname: Liu
authors.forename: Lantong
authors.initials: L
authors.lastname: Zhang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D034181:Epimedium / Q000737:chemistry / Q000378:metabolism; D005419:Flavonoids / Q000652:urine*; D036145:Ginsenosides / Q000652:urine*; D008517:Phytotherapy; D028321:Plant Preparations; D051381:Rats; D012503:Saponins / Q000032:analysis; D014466:Ultraviolet Rays
chemical_list: D005419:Flavonoids; D036145:Ginsenosides; D028321:Plant Preparations; D012503:Saponins