title: Proficiency testing outcomes of 3-hydroxyisovalerylcarnitine measurements by tandem mass spectrometry in newborn screening.
abstract: BACKGROUND
  The use of tandem mass spectrometry (MS/MS) for the analysis of amino acids and acylcarnitines from dried-blood spots (DBS) has become routine practice in newborn screening laboratories. The Newborn Screening Quality Assurance Program (NSQAP) added 3-hydroxyisovalerylcarnitine (C5OH) into its routine quality control and proficiency testing (PT) DBS materials for MS/MS to assure the quality of C5OH screening. We report the results from NSQAP evaluations for C5OH-enriched DBS, and summarize participant screening practices based on their analytical methods.
  
  
  METHODS
  NSQAP prepared C5OH-enriched DBS materials for its participants. Laboratories reported quantitative and qualitative results. Bias plots of quantitative results were constructed using reported data and the results were sorted by an analytical method.
  
  
  RESULTS
  NSQAP participants reported PT specimen 3964 as outside of normal limits for C5OH. The mean C5OH value for derivatized and non-derivatized methods was 2.80 and 2.67 μmol/l, respectively. Reported data from other specimens showed a similar trend in derivatized vs. non-derivatized assay results. Differences in C5OH quantitative values were observed among laboratories using different internal standards.
  
  
  CONCLUSIONS
  C5OH MS/MS measurements in DBS assays varied by method and the choice of internal standards. The use of NSQAP's DBS materials allows harmonization of C5OH measurements by newborn screening laboratories worldwide.
authors.affiliation: Newborn Screening Quality Assurance Program, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mail Stop F-19, Atlanta, GA 30341, United States. tlim@cdc.gov
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Lim
authors.forename: Víctor R
authors.initials: VR
authors.lastname: De Jesús
authors.forename: Nancy K
authors.initials: NK
authors.lastname: Meredith
authors.forename: Maya R
authors.initials: MR
authors.lastname: Sternberg
authors.forename: Donald H
authors.initials: DH
authors.lastname: Chace
authors.forename: Joanne V
authors.initials: JV
authors.lastname: Mei
authors.forename: W Harry
authors.initials: WH
authors.lastname: Hannon
mesh_terms: D002331:Carnitine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D015997:Neonatal Screening; D010865:Pilot Projects; D011785:Quality Assurance, Health Care; D012016:Reference Values; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C098116:3-hydroxyisovalerylcarnitine; D002331:Carnitine