title: A RhoA-derived peptide inhibits human immunodeficiency virus-1 entry in vitro.
abstract: RhoA-derived peptides have been shown to have antiviral activity against both human respiratory syncytial virus and human parainfluenza virus-3. The present study investigates the toxicity, anti-HIV-1 activity and mechanism of action of a RhoA-derived peptide (RhoA 77-95). The efficacy of this peptide was compared to a scrambled peptide of the same amino acid composition and Enfuvirtide, a HIV entry inhibitor. Our data show that this RhoA-derived peptide is a non-toxic and effective inhibitor of a CXCR4 tropic strain of HIV-1. We also demonstrate that the mechanism of entry inhibition is likely mediated by polyanionic properties and is dependent on the dimerization of peptides.
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon 973318, USA.
authors.forename: Maciej
authors.initials: M
authors.lastname: Maselko
authors.forename: Casey
authors.initials: C
authors.lastname: Ward
authors.forename: Manoj
authors.initials: M
authors.lastname: Pastey
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D000077560:Enfuvirtide; D015700:HIV Envelope Protein gp41 / Q000494:pharmacology; D023581:HIV Fusion Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009842:Oligopeptides / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity; D010446:Peptide Fragments / Q000494:pharmacology; D055503:Protein Multimerization; D053586:Virus Internalization / Q000187:drug effects*; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D015700:HIV Envelope Protein gp41; D023581:HIV Fusion Inhibitors; D009842:Oligopeptides; D010446:Peptide Fragments; C066737:RHOA protein, human; D000077560:Enfuvirtide; D020742:rhoA GTP-Binding Protein