title: [Effects of splenic B lymphocyte proliferation and response and intracellular Ca2+ of hsBAFF in mice].
abstract: OBJECTIVE
  To investigate effects of hsBAFF synthesized in Escherichia coli on spleen B lymphocyte immune response and its intracellular free Ca2+ ([Ca2]i]) signaling in mice.
  
  
  METHODS
  Twenty ICR mice, half males-half females, were chosen and randomly divided into a normal control group (n=10) and a hsBAFF treatment group (n-10). The mice in hsBAFF treatment group were given abdominal cavity injection of hsBAFF solution which was diluted with phosphate buffered saline (PBS) at dosage of 0.1 mg/kg body weight once each day for over eight days. The mice in control group were received abdominal injection of PBS at the same dose and frequency. Spleen B lymphocyte proliferation and its immune response to LPS stimulation in mice were evaluated using an MTT assay, and change of spleen B lymphocyte [Ca2+]i was assayed under a laser scanning confocal microscope.
  
  
  RESULTS
  B lymphocyte proliferation and its immune response to LPS stimulation were significantly higher in hsBAFF-treated mice than in control mice (P < 0.05). The B lymphocyte [Ca2+]i fluorescence intensity in hsBAFF-treated mice maintained at a relatively high level fluctuation, and its average intensity was significantly higher to that of control mice (P < 0.01), but change rate of the intensity was lower compared to that of control group.
  
  
  CONCLUSIONS
  hsBAFF synthesized in Escherichia coli can enhance immune function in the body by increasing B lymphocyte proliferation and its immune response. hsBAFF-activated B lymphocyte function may be associated with increasing B lymphocytes [Ca2+]i.
authors.affiliation: College of Life Sciences, Nanjing Normal University, Jiangsu Engineering Research Center for Bio-medical Function Materials, Nanjing 210097, China.
authors.forename: Xiao-man
authors.initials: XM
authors.lastname: Yang
authors.forename: Xiao-xiao
authors.initials: XX
authors.lastname: Shan
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.forename: Shuang-quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D053264:B-Cell Activating Factor / Q000276:immunology / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D053264:B-Cell Activating Factor; D002118:Calcium