title: Regulation of protein expression by L-arginine in endothelial cells.
abstract: L-Arginine is a conditionally essential amino acid for humans and plays an important role in the regulation of cardiovascular function and antioxidative defense. Previous studies have focused on the important role of L-arginine as a physiological precursor in the generation of nitric oxide and polyamines in endothelial cells (cells that line the interior surface of blood vessels). Because of the rapid development of high-throughput proteomics technology, there is now growing interest in studying roles for L-arginine in modulating endothelial-cell protein expression. Of particular interest, recent proteomics analysis has shown that treatment of coronary venular endothelial cells with a physiological level of L-arginine (e.g., 0.1 mM) increases expression of structural proteins (vimentin and tropomyosin) and cytochrome bc1 complex iii-chain A, while decreasing expression of stress-related proteins (PDZ domain containing-3), in these cells. These findings aid in elucidating the mechanisms responsible for the beneficial effect of physiological levels of L-arginine on the circulatory system.
authors.affiliation: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China 100193.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Lei
authors.forename: Cuiping
authors.initials: C
authors.lastname: Feng
authors.forename: Chuang
authors.initials: C
authors.lastname: Liu
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Meininger
authors.forename: Fenglai
authors.initials: F
authors.lastname: Wang
authors.forename: Defa
authors.initials: D
authors.lastname: Li
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D001120:Arginine / Q000378:metabolism* / Q000494:pharmacology; D003331:Coronary Vessels / Q000166:cytology / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D014176:Protein Biosynthesis; D040901:Proteomics; D014335:Tropomyosin / Q000096:biosynthesis; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D014335:Tropomyosin; D014746:Vimentin; D001120:Arginine