title: [Effects of free fatty acids on insulin signaling proteins in rat islet cells].
abstract: OBJECTIVE
  To investigate whether free fatty acid impair insulin signaling proteins to inhibit insulin action in rat islet cells.
  
  
  METHODS
  After rat islet cells were incubated with palmitate (0.25 mmol/L) or oleate (0.125 mmol/L) for 12 hours, 24 hours and 36 hours, western bolt was used to assess the protein abundance of cPKCalpha, Grb2 and FERK2.
  
  
  RESULTS
  The protein content of cPKCalpha were significantly upregulated and the protein abundance of Grb2 and ERK2 was decreased comparing with control in rat islet cells after incubated with free fatty acids.
  
  
  CONCLUSIONS
  Free fatty acids may inhibit insulin activity in rat islet cells through up-regulating the expression of cPKCalpha or down-regulating the expression of Grb2 and ERK2.
authors.affiliation: Departmnent of Endocrinology, Navy General Hospital, Beijing 100037, China.
authors.forename: Qi-Yu
authors.initials: QY
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Cong
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D051380:GRB2 Adaptor Protein / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D005230:Fatty Acids, Nonesterified; D051380:GRB2 Adaptor Protein; C495950:Grb2 protein, rat; D007328:Insulin; D051571:Protein Kinase C-alpha; D019950:Mitogen-Activated Protein Kinase 1