title: Preparation and characterization of microporous poly(D,L-lactic acid) film for tissue engineering scaffold.
abstract: We prepared a series of microporous films based on poly(d,l-lactic acid) (PLA) via phase separation. According to scanning electron microscopy (SEM), a 3-dimensional foamy structure with multimicrometer scale pores on the air surface of film could be observed. As the morphology of PLA film could not be stabilized using solvent-nonsolvent phase separation, we investigated the effect of temperature, air movement, and concentration on the properties of microporous PLA films. The results show that when the temperature was 25°C in a vacuum, it was easy to prepare PLA film with micropores, and it was stable. As the relationship between the morphology and formation factors was clear and the morphology of the PLA film was controllable, we studied the PLA film's potential use for cell culture. SEM results showed that NIH3T3 cell could be adhered on the surface of film well after incubation for 2 days. Meanwhile, in vitro culture experiments revealed the great biocompatibility of the scaffold for adsorption and proliferation of fibroblasts.
authors.affiliation: State Key Lab of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan, University, Chengdu, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.forename: Xiu Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Min
authors.initials: M
authors.lastname: Fan
authors.forename: Mei Juan
authors.initials: MJ
authors.lastname: Huang
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Qian
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014618:Vacuum
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)