title: The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.
abstract: A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.
authors.affiliation: Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Ting
authors.initials: T
authors.lastname: Ni
authors.forename: Kang
authors.initials: K
authors.lastname: Tu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Shen
authors.initials: S
authors.lastname: Song
authors.forename: Han
authors.initials: H
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Xie
authors.forename: Kristin C
authors.initials: KC
authors.lastname: Scott
authors.forename: Shiv I
authors.initials: SI
authors.lastname: Grewal
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
mesh_terms: D004257:DNA Polymerase II / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005799:Genes, Dominant; D005800:Genes, Fungal; D016681:Genome, Fungal; D006657:Histones / Q000235:genetics; D008540:Meiosis; D008957:Models, Genetic; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D006657:Histones; C493530:Htz1 protein, S cerevisiae; D016376:Oligonucleotides, Antisense; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D004257:DNA Polymerase II