title: Translocation of intratracheally instilled multiwall carbon nanotubes to lung-associated lymph nodes in rats.
abstract: In order to assess the extrapulmonary effects of multiwall carbon nanotubes (MWCNT), deposition of MWCNT and histopathologic changes in lung-associated lymph nodes (LALN) were examined in MWCNT-administered rats. At the age of 13 wk, male F344 rats were intratracheally instilled with MWCNT at a dose of 0 (vehicle), 40 or 160 μg/rat. The rats were sacrificed on Day 1, 7, 28 or 91 after instillation and light microscopic examinations were performed on LALN tissues. MWCNT was translocated to right and left posterior mediastinal lymph nodes and parathymic lymph nodes. Deposition of MWCNT was greater in the posterior mediastinal lymph node than in the parathymic lymph node, and the amount of MWCNT deposited in these two lymph nodes increased gradually and dose-dependently with time. MWCNT was phagocytosed by nodal macrophages, and some of the MWCNT-laden macrophages were aggregated. Transmission electron microscopic (TEM) observation confirmed the presence of MWCNT fibers with a characteristic multi-walled cylindrical structure.
authors.affiliation: Japan Bioassay Research Center, Japan Industrial Safety and Health Association, 2445 Hirasawa, Hadano, Kanagawa 257-0015, Japan. s-aiso@jisha.or.jp
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Aiso
authors.forename: Hisayo
authors.initials: H
authors.lastname: Kubota
authors.forename: Yumi
authors.initials: Y
authors.lastname: Umeda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kasai
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Takaya
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Kasuke
authors.initials: K
authors.lastname: Nagano
authors.forename: Toshio
authors.initials: T
authors.lastname: Sakai
authors.forename: Shigeki
authors.initials: S
authors.lastname: Koda
authors.forename: Shoji
authors.initials: S
authors.lastname: Fukushima
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008198:Lymph Nodes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D037742:Nanotubes, Carbon / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D037742:Nanotubes, Carbon