title: Acanthamoeba T3, T4 and T5 in swimming-pool waters from Southern Brazil.
abstract: Species of Acanthamoeba, known to cause keratitis (AK) and granulomatous encephalitis in humans are frequently isolated from a variety of water sources. In this study, 13 Acanthamoeba isolates from swimming pools were classified at the genotype level based on the sequence analysis of the Acanthamoeba small-subunit rRNA gene. Nine of the 13 isolates were genotype T5, three were genotype T4, and one was T3. Several genotypes have been reported worldwide as causative agents of AK, including genotypes T3, T4, and T5. The present study indicates that genotype T5 is a common contaminant in swimming-pool water.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitogia, Instituto de Ciências Básicas da Saúde, Setor de Parasitologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, Porto Alegre, RS, Brazil. kcaumo@yahoo.com.br
authors.forename: Karin
authors.initials: K
authors.lastname: Caumo
authors.forename: Marilise B
authors.initials: MB
authors.lastname: Rott
mesh_terms: D000048:Acanthamoeba / Q000145:classification* / Q000235:genetics*; D015823:Acanthamoeba Keratitis / Q000469:parasitology; D015373:Bacterial Typing Techniques; D001938:Brazil; D004660:Encephalitis / Q000469:parasitology; D005838:Genotype; D006105:Granulomatous Disease, Chronic / Q000469:parasitology; D006801:Humans; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017421:Sequence Analysis; D013551:Swimming Pools; D014871:Water Microbiology
chemical_list: D012337:RNA, Ribosomal, 18S