title: Single-cell whole-genome amplification technique impacts the accuracy of SNP microarray-based genotyping and copy number analyses.
abstract: Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number of commercially available WGA kits have been independently utilized in previous single-cell microarray studies. However, direct comparison of their performance on single cells has not been conducted. The present study demonstrates that among previously published methods, a single-cell GenomePlex WGA protocol provides the best combination of speed and accuracy for single nucleotide polymorphism microarray-based copy number (CN) analysis when compared with a REPLI-g- or GenomiPhi-based protocol. Alternatively, for applications that do not have constraints on turnaround time and that are directed at accurate genotyping rather than CN assignments, a REPLI-g-based protocol may provide the best solution.
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA. ntreff@rmanj.com
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.forename: Lesley E
authors.initials: LE
authors.lastname: Northrop
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
mesh_terms: D002460:Cell Line; D016172:DNA Fingerprinting / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D018628:Gene Dosage; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D007621:Karyotyping; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide