title: Microvascular oxygen tension and flow measurements in rodent cerebral cortex during baseline conditions and functional activation.
abstract: Measuring cerebral oxygen delivery and metabolism microscopically is important for interpreting macroscopic functional magnetic resonance imaging (fMRI) data and identifying pathological changes associated with stroke, Alzheimer's disease, and brain injury. Here, we present simultaneous, microscopic measurements of cerebral blood flow (CBF) and oxygen partial pressure (pO(2)) in cortical microvessels of anesthetized rats under baseline conditions and during somatosensory stimulation. Using a custom-built imaging system, we measured CBF with Fourier-domain optical coherence tomography (OCT), and vascular pO(2) with confocal phosphorescence lifetime microscopy. Cerebral blood flow and pO(2) measurements displayed heterogeneity over distances irresolvable with fMRI and positron emission tomography. Baseline measurements indicate O(2) extraction from pial arterioles and homogeneity of ascending venule pO(2) despite large variation in microvessel flows. Oxygen extraction is linearly related to flow in ascending venules, suggesting that flow in ascending venules closely matches oxygen demand of the drained territory. Oxygen partial pressure and relative CBF transients during somatosensory stimulation further indicate arteriolar O(2) extraction and suggest that arterioles contribute to the fMRI blood oxygen level dependent response. Understanding O(2) supply on a microscopic level will yield better insight into brain function and the underlying mechanisms of various neuropathologies.
authors.affiliation: Department of Radiology, MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Yaseen
authors.forename: Vivek J
authors.initials: VJ
authors.lastname: Srinivasan
authors.forename: Sava
authors.initials: S
authors.lastname: Sakadžić
authors.forename: Harsha
authors.initials: H
authors.lastname: Radhakrishnan
authors.forename: Iwona
authors.initials: I
authors.lastname: Gorczynska
authors.forename: Weicheng
authors.initials: W
authors.lastname: Wu
authors.forename: James G
authors.initials: JG
authors.lastname: Fujimoto
authors.forename: David A
authors.initials: DA
authors.lastname: Boas
mesh_terms: D000818:Animals; D001160:Arterioles / Q000502:physiology; D002196:Capillaries / Q000378:metabolism*; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism* / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D003627:Data Interpretation, Statistical; D055724:Electrophysiological Phenomena; D008279:Magnetic Resonance Imaging; D008297:Male; D018613:Microscopy, Confocal; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000502:physiology*; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000502:physiology; D041623:Tomography, Optical Coherence; D014699:Venules / Q000502:physiology
chemical_list: D010100:Oxygen