title: Peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects.
abstract: Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects on bone repair in a unicortical defect model. Treatment of mesenchymal cells produced an increase in alkaline phosphatase activity, showed nodule formation by the cells, and increased the expression of genes for runx2, osterix, bone sialoprotein, and osteocalcin. A collagen sponge soaked with peptide promoted repair of bone defects, whereas the control was less effective. The results from this study demonstrated that mesenchymal cells treated with peptide in vitro differentiate towards osteogenesis, and, that peptides delivered in vivo using a collagen sponge promote the repair of unicortical defects.
authors.affiliation: Orthopaedics Research, University of Virginia, USA. gb@virginia.edu
authors.forename: Gary
authors.initials: G
authors.lastname: Balian
authors.forename: Gina
authors.initials: G
authors.lastname: Beck
authors.forename: Vedavathi
authors.initials: V
authors.lastname: Madhu
authors.forename: Robert
authors.initials: R
authors.lastname: Sikes
authors.forename: Quanjun
authors.initials: Q
authors.lastname: Cui
authors.forename: Haixiang
authors.initials: H
authors.lastname: Liang
authors.forename: Joshua
authors.initials: J
authors.lastname: Bush
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D001861:Bone Regeneration / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D019151:Peptide Library; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D019151:Peptide Library; D010455:Peptides