title: Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix.
abstract: The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.
authors.affiliation: Department of Oral and Maxillofacial Surgery and Traumatology and Periodontology, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Annelissa Zorzeto
authors.initials: AZ
authors.lastname: Rodrigues
authors.forename: Paulo Tambasco de
authors.initials: PT
authors.lastname: Oliveira
authors.forename: Arthur Belém
authors.initials: AB
authors.lastname: Novaes
authors.forename: Luciana Prado
authors.initials: LP
authors.lastname: Maia
authors.forename: Sérgio Luís Scombatti de
authors.initials: SL
authors.lastname: Souza
authors.forename: Daniela Bazan
authors.initials: DB
authors.lastname: Palioto
mesh_terms: D020341:Absorbable Implants; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005881:Gingiva / Q000166:cytology*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods; D006801:Humans; D017768:Skin, Artificial; D018709:Statistics, Nonparametric; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: C440568:Alloderm; D001672:Biocompatible Materials; D003094:Collagen