title: More mesenchymal stem cells enriched from bone marrow aspirates by culturing bone marrow particles and mononuclear cells separately.
abstract: Bone marrow (BM) is the major source of mesenchymal stem cells (MSC). In most experiments, MSC were classically cultured from mononuclear cells (MNC) isolated by density gradient centrifugation method. However, several studies have demonstrated that this method was less efficient for MSC recovery. This study was aimed to investigate whether BM particles were the cause resulting in less efficiency of this method and how to isolate them. A total of 20 patients were enrolled in this study. MNC were cultured by standard adherence and BM particles were cultivated by primary explant culture. For BM from patients 1-10, MNC were first isolated and BM particles were then filtered out. The morphology and the fibroblastic colony number were compared between cultures of MNC and BM particles. For BM from patients 11-20, MNC isolation and BM particle filtration were processed in opposite order, then the immunophenotype and function between adherent cells expanded from MNC and BM particles were compared. In addition, for patients 11-20, the left BM aspirates were cultured too after BM particles and MNC were isolated separately. The results showed that adherent cells from BM particles were MSC. After BM particles were filtered out and cultured separately, MSC could be recovered completely from MNC isolated by density gradient centrifugation and no MSC were left in the residual BM aspirates. BM particles, which have been mostly discarded by the method of density gradient centrifugation, are another important source of MSC and they can be cultivated reliably by primary explant culture. It is concluded that more MSC are recovered from a single BM sample by culturing BM particles and MNC separately.
authors.affiliation: Chinese Academy of Medical Sciences, Tianjin 300020, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xing
authors.forename: Peng-Xia
authors.initials: PX
authors.lastname: Liu
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.forename: Shao-Guang
authors.initials: SG
authors.lastname: Yang
authors.forename: Qin-Jun
authors.initials: QJ
authors.lastname: Zhao
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Li
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Lu
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Ren
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D055815:Young Adult