title: The transcription of l-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbate, during development of Persian sturgeon Acipenser persicus.
abstract: l-Gulono-gamma-lactone oxidase (GULO) is a key enzyme for the biosynthesis of ascorbate, which is essential for several cellular functions. In the present study, mRNA expression of GULO gene was evaluated during the early development of Persian sturgeon. First, because there are no comparative studies that have established suitable quantitative real-time PCR reference genes in sturgeons for any physiological conditions, we evaluated six candidate reference genes (ACTB, RPL13, UBQ, RPL6, GAPDH and EF1A) during the early development of Persian sturgeon. The most stable mRNA expression was obtained with RPL6 and ACTB, whereas the least stable was RPL13. After normalization using RPL6, ACTB and RPL6/ACTB combination, the mRNA expression of GULO was highest at the embryonic stage (2days before hatching; P<0.05) and started to decline from hatching of larvae to the rest of the developmental time-points. This suggests that the vitamin C requirements are highest during early life stages, and it is likely that the changes in GULO mRNA expression are associated with changes in GULO enzyme activity.
authors.affiliation: Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran. akbarzadeh@ut.ac.ir
authors.forename: Arash
authors.initials: A
authors.lastname: Akbarzadeh
authors.forename: Hamid
authors.initials: H
authors.lastname: Farahmand
authors.forename: Frouzandeh
authors.initials: F
authors.lastname: Mahjoubi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Nematollahi
authors.forename: Piia
authors.initials: P
authors.lastname: Leskinen
authors.forename: Kalle
authors.initials: K
authors.lastname: Rytkönen
authors.forename: Mikko
authors.initials: M
authors.lastname: Nikinmaa
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis; D005399:Fishes / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D050544:L-Gulonolactone Oxidase / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000592:standards; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D012015:Reference Standards; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid