title: Design, synthesis and DNA binding properties of orthogonally positioned diamino containing polyamide f-IPI.
abstract: An orthogonally positioned diamino/dicationic polyamide f-IPI 2 was synthesized. It has enhanced binding affinity, and it showed comparable sequence specificity to its monoamino/monocationic counterpart f-IPI 1. Results from CD and DNase I footprinting studies confirmed the minor groove binding and selectivity of polyamides 1 and 2 for the cognate sequence 5'-ACGCGT-3'. SPR studies provided their binding constants: 2.4 × 10(8)M(-1) for diamino 2, which is ∼4 times higher than 5.4 × 10(7)M(-1) for its monoamino analogue 1.
authors.affiliation: Division of Natural & Applied Sciences and Department of Chemistry, Hope College, 35 East 12th Street, Holland, MI 49423, USA.
authors.forename: Balaji
authors.initials: B
authors.lastname: Babu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Adam
authors.initials: A
authors.lastname: Plaunt
authors.forename: Camille
authors.initials: C
authors.lastname: Riddering
authors.forename: Ross
authors.initials: R
authors.lastname: Ogilvie
authors.forename: Laura
authors.initials: L
authors.lastname: Westrate
authors.forename: Ryan
authors.initials: R
authors.lastname: Davis
authors.forename: Amanda
authors.initials: A
authors.lastname: Ferguson
authors.forename: Hilary
authors.initials: H
authors.lastname: Mackay
authors.forename: Toni
authors.initials: T
authors.lastname: Rice
authors.forename: Sameer
authors.initials: S
authors.lastname: Chavda
authors.forename: David
authors.initials: D
authors.lastname: Wilson
authors.forename: Shicai
authors.initials: S
authors.lastname: Lin
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Kiakos
authors.forename: John A
authors.initials: JA
authors.lastname: Hartley
authors.forename: Moses
authors.initials: M
authors.lastname: Lee
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000737:chemistry; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; C556451:polyamide f-IPI; D004247:DNA; D003850:Deoxyribonuclease I