title: Selective suppression of two-photon fluorescence in laser scanning microscopy by ultrafast pulse-train excitation.
abstract: Selective excitation of a particular fluorophore in the presence of others demands clever design of the optical field interacting with the molecules. We describe the use of 20- to 50-GHz pulse-train excitation leading to two-photon absorption, followed by successive one-photon stimulated emission as a potential technique in the context of controlling two-photon molecular fluorescence, with applications in microscopy.
authors.affiliation: Indian Institute of Technology Kanpur, Department of Chemistry, UP-208016, India.
authors.forename: Arijit Kumar
authors.initials: AK
authors.lastname: De
authors.forename: Debjit
authors.initials: D
authors.lastname: Roy
authors.forename: Debabrata
authors.initials: D
authors.lastname: Goswami
mesh_terms: D016477:Artifacts; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007368:Interferometry / Q000295:instrumentation*; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D019047:Phantoms, Imaging; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*