title: Molecular epidemiology of Plum pox virus in Japan.
abstract: For a molecular epidemiological study based on complete genome sequences, 37 Plum pox virus (PPV) isolates were collected from the Kanto region in Japan. Pair-wise analyses revealed that all 37 Japanese isolates belong to the PPV-D strain, with low genetic diversity (less than 0.8%). In phylogenetic analysis of the PPV-D strain based on complete nucleotide sequences, the relationships of the PPV-D strain were reconstructed with high resolution: at the global level, the American, Canadian, and Japanese isolates formed their own distinct monophyletic clusters, suggesting that the routes of viral entry into these countries were independent; at the local level, the actual transmission histories of PPV were precisely reconstructed with high bootstrap support. This is the first description of the molecular epidemiology of PPV based on complete genome sequences.
authors.affiliation: Department of Agricultural and Environmental Biology, University of Tokyo, Tokyo, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Maejima
authors.forename: Misako
authors.initials: M
authors.lastname: Himeno
authors.forename: Ken
authors.initials: K
authors.lastname: Komatsu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takinami
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Yamaji
authors.forename: Kenro
authors.initials: K
authors.lastname: Oshima
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba
mesh_terms: D001483:Base Sequence; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics*; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000706:statistics & numerical data / Q000821:virology*; D017801:Plum Pox Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D027861:Prunus / Q000821:virology*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral