title: [Experimental studies of genistein on the proliferation and apoptosis of human prolactinoma cells cultured in vitro].
abstract: OBJECTIVE
  To study the influence of genistein (GST) on the proliferation and apoptosis of cultured human prolactinoma cells.
  
  
  METHODS
  MTT method and 3H-TdR incorporation and cell cycle analysis were used to examine the changes of proliferation and DNA synthesis of human prolactinoma cells under influence of GST and beta-estradiol (E2). Tdt-mediated dUTP nick end labeling (TUNEL) were employed to observe the effect of GST and E2 on the apoptosis of human prolactinoma cells.
  
  
  RESULTS
  In a dose dependent manner, GST of different concentration could significantly inhibit the proliferation of human prolactinoma cells cultured in vitro. GST(10(-5) mol/L) could increase the proportion of cells in G1 phase from 55.3% up to 90.3%. E2 of different concentration could dose-dependently increase the proliferation of human prolactinoma cells. E (10(-5) mol/L) could increase the proportion of cells in G2 phase from 15.6% up to 41.8%. However, a lower suppressive proliferation of cultured human prolactinoma cells was observed with GST and E2 together. GST, not E2, could significantly induce the apoptosis of human prolactinoma cells cultured in vitro.
  
  
  CONCLUSIONS
  GST inhibits the proliferation, DNA synthesis and cell cycle of cultured human pituitary cells, and induces its apoptosis. E2 decreases partly the effect of GST on the suppression of proliferation, not apoptotic induction, of human prolactinoma cells cultured in vitro.
authors.affiliation: Department of Physiology, Faculty of Preclinical Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Chi
authors.forename: C X
authors.initials: CX
authors.lastname: Li
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhu
authors.forename: Y L
authors.initials: YL
authors.lastname: Liu
authors.forename: J W
authors.initials: JW
authors.lastname: Gu
authors.forename: J K
authors.initials: JK
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D015175:Prolactinoma / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D004958:Estradiol; D019833:Genistein