title: Antioxidant capacity of binuclear Cu(II)-cyclophanes, insights from two synthetic bioactive molecules.
abstract: The compounds 2,9,25,32-tetraoxo-4,7,27,30-tetrakis(carboxymethyl)-1,4,7,10,24,27,30,33-octaaza-17,40-dioxa[10.1.10.1]paracyclophane and 2,9,25,32-tetraoxo-4,7,27,30-tetrakis(carboxymethyl)-1,4,7,10,24,27,30,33-octaaza[10.1.10.1]paracyclophane binuclear copper complexes (Cu2PO and Cu2PC, respectively) were studied by determining their antioxidant capacity using the TROLOX equivalent antioxidant capacity (TEAC) assay, and their cytotoxicity on cultured cells, as well as the superoxide dismutase (SOD)-like activity. Cu2PO had an antioxidant capacity (0.1 g eq TROLOX mol−1) within the order of magnitude of ascorbic acid, and both, Cu2PO and Cu2PC were nontoxic to cultured peripheral mononuclear blood cells. The SOD-like activity was evaluated using the nitroblue tetrazolium assay, and both compounds presented an excellent activity: for Cu2PO, the IC50 was 52 nM and for Cu2PC an IC50 of 0.5 μM was obtained comparable to CuZn SOD IC50 17 nM (Fernandes et al., J Inorg Biochem 2007;101:849–858). These results suggest that synthetic binuclear macrocycles are good candidates to be used as synthetic bioactive molecules with applications in biomedicine.
authors.affiliation: Departamento de Investigación en Polímeros y Materiales (DIPM). Universidad de Sonora, Calle Rosales y Blvd. Luis Encinas s/n, Col. Centro, PO Box 130. Hermosillo Sonora, 83000, México.
authors.forename: Rocio
authors.initials: R
authors.lastname: Sugich-Miranda
authors.forename: Rogerio R
authors.initials: RR
authors.lastname: Sotelo-Mundo
authors.forename: Erika
authors.initials: E
authors.lastname: Silva-Campa
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Gonzalez-Aguilar
authors.forename: Enrique F
authors.initials: EF
authors.lastname: Velazquez-Contreras
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D002478:Cells, Cultured; D002839:Chromans / Q000378:metabolism; D056831:Coordination Complexes / Q000378:metabolism / Q000633:toxicity; D003300:Copper / Q000737:chemistry*; D004988:Ethers, Cyclic / Q000378:metabolism / Q000633:toxicity*; D006801:Humans; D047028:Macrocyclic Compounds / Q000378:metabolism / Q000633:toxicity*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010880:Piperidines / Q000378:metabolism / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C557488:(2,9,25,32-tetraoxo-4,7,27,30-tetrakis(carboxymethyl)-1,4,7,10,24,27,30,33-octaaza(10.1.10.1)paracyclophane)copper(II); C557487:(2,9,25,32-tetraoxo-4,7,27,30-tetrakis(carboxymethyl)-1,4,7,10,24,27,30,33-octaaza-17,40-dioxa(10.1.10.1)paracyclophane)copper(II); D000975:Antioxidants; D002839:Chromans; D056831:Coordination Complexes; D004988:Ethers, Cyclic; D047028:Macrocyclic Compounds; D010880:Piperidines; D009580:Nitroblue Tetrazolium; D003300:Copper; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid