title: [Expression of interleukin-2 receptors on breast cancer cell line MCF-7 cells].
abstract: OBJECTIVE
  To investigate the expression of interleukin-2 receptors (IL-2Rs) on MCF-7 cells, estradiol's regulation of IL-2Rs expression and the influence of IL-2 on the proliferation of MCF-7 cells.
  
  
  METHODS
  Immunocytochemistry and flow cytometric analysis were used to investigate the expression of interleukin-2 receptors (IL-2Rs) by using of specific IL-2R polyclonal antibody; MTT method and 3H-TdR incorporation method were used to examine the changes of proliferation of MCF-7 cells.
  
  
  RESULTS
  IL-2Ralpha, beta, gamma like immunoreactive substances can be found on MCF-7 cells and the IL-2Rgamma immunostaining was more strong than the other two. Estradiol of 10(-6) mol/L can increase the percentage of immunoreactive cells of IL-2Ralpha, beta and the expression of IL-2Rgamma. Exogenous addition of recombinant IL-2 of 100 U/ml to 1 000 U/ml can significantly increase the proliferation of MCF-7 cells.
  
  
  CONCLUSIONS
  MCF-7 cell can express IL-2R and estradiol can regulate their expression, IL-2 can influence the proliferation of MCF-7 cells.
authors.affiliation: Department of Physiology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Zhong
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Zhu
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Wang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D015375:Receptors, Interleukin-2 / Q000378:metabolism*
chemical_list: D015375:Receptors, Interleukin-2; D004958:Estradiol