title: Effect of low levels of aflatoxin B₁ on performance, biochemical parameters, and aflatoxin B₁ in broiler liver tissues in the presence of monensin and sodium bentonite.
abstract: Aflatoxins (AF) are a major problem in broiler production and are significant economic and public health burdens worldwide. A commercial sodium bentonite (Na-B) adsorbent was used to prevent the effect of AF [50 µg of aflatoxin B₁ (AFB₁)/kg of feed] in broiler productivity, biochemical parameters, macroscopic and microscopic liver changes, and AFB₁ liver residues. The influence of Na-B (0.3%) and monensin (MON, 100 mg/kg), alone or in combination, was investigated in depth. The dietary treatments were as follows: treatment (T) 1: basal diet (B); T2: B + MON; T3: B + Na-B; T4: B + Na-B + MON; T5: B + AFB₁; T6: B + AFB₁ + Na-B + MON; T7: B + AFB₁ + MON; T8: B + AFB₁ + Na-B. Birds were fed dietary treatments for 28 d (d 18 to 46). No significant differences (P < 0.05) were observed among treatments with respect to broiler performance, biochemical parameters, or relative liver weights. With the exception of T8, all livers showed histopathological alterations, with accumulation of fat vacuoles. The normal appearance of livers from T8 showed the protective effect of Na-B against aflatoxicosis. The residual AFB₁ levels in livers from T5 to T8 ranged from 0.2 to 1.0 ng/g and were higher in livers from T6 (P < 0.05). Results of this study indicate a competition between AFB₁ and MON for adsorption sites on Na-B when feed contains low levels of the toxin, indicating a nonselective adsorption capacity of this particular Na-B. In addition, significant levels of AFB₁ in livers indicate that this determination is an important technique not only for diagnosis of aflatoxicosis in broilers, but also for quality control of avian products.
authors.affiliation: Departamento de Química, Facultad de Ciencias Exactas, Físico, Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional No. 36 Km. 601, Río Cuarto, Córdoba, Argentina.
authors.forename: A P
authors.initials: AP
authors.lastname: Magnoli
authors.forename: M P
authors.initials: MP
authors.lastname: Monge
authors.forename: R D
authors.initials: RD
authors.lastname: Miazzo
authors.forename: L R
authors.initials: LR
authors.lastname: Cavaglieri
authors.forename: C E
authors.initials: CE
authors.lastname: Magnoli
authors.forename: C I
authors.initials: CI
authors.lastname: Merkis
authors.forename: A L
authors.initials: AL
authors.lastname: Cristofolini
authors.forename: A M
authors.initials: AM
authors.lastname: Dalcero
authors.forename: S M
authors.initials: SM
authors.lastname: Chiacchiera
mesh_terms: D000327:Adsorption; D016604:Aflatoxin B1 / Q000032:analysis / Q000633:toxicity*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000931:Antidotes / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology; D001546:Bentonite / Q000494:pharmacology*; D002645:Chickens; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000737:chemistry*; D008297:Male; D008985:Monensin / Q000494:pharmacology*; D011201:Poultry Diseases / Q000139:chemically induced*
chemical_list: D000931:Antidotes; D000981:Antiprotozoal Agents; D001546:Bentonite; D008985:Monensin; D016604:Aflatoxin B1