title: Control of aerial mycelium formation by the BldK oligopeptide ABC transporter in Streptomyces griseus.
abstract: An oligopeptide permease family ATP-binding cassette (ABC) transporter encoded by SGR2418-SGR2414 was shown to be essential for aerial mycelium formation on glucose-containing media in Streptomyces griseus. In spite of only weak sequence similarity, the operon was equivalent to the bldK operon of Streptomyces coelicolor A3(2) in terms of chromosomal location and function. Transcription of the operon appeared not to be directly regulated by AdpA, a global regulator of morphological and physiological development in S. griseus, although it was affected by adpA inactivation. This study revealed that an ABC transporter was essential for aerial mycelium formation not only in S. coelicolor A3(2) but also in S. griseus, indicating that extracellular signaling by certain peptides should be conserved among different Streptomyces species.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Akanuma
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ueki
authors.forename: Morio
authors.initials: M
authors.lastname: Ishizuka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001692:Biological Transport; D024881:Drug Resistance, Bacterial; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D048372:Streptomyces coelicolor / Q000235:genetics; D013305:Streptomyces griseus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters; C401645:AdpA protein, Streptomyces griseus; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D009943:Organophosphorus Compounds; D015534:Trans-Activators; C003122:bialaphos; C037230:oligopeptide permease, Bacteria