title: [Mechanisms of protection effect of bcl-2 gene transfection on heat-stressed cardiomyocytes].
abstract: OBJECTIVE
  To study the mechanisms of protection of bcl-2 gene transfection against heat-stressed cardiomyocytes.
  
  
  METHODS
  Cardiomyocytes were isolated and cultured. bcl-2 was transfected into cardiomyocytes with Lipofectamine transfection methods. The cardiomyocytes were stressed by heat. The change of H+ -ATPase synthesis activity of cardiomyocytes mitochondria caused by bcl-2 transfection was measured by chemical radiation method. The changes of Caspase 3 activity of cardiomyocytes caused by bcl-2 transfection was measured by fluorometric analysis.
  
  
  RESULTS
  bcl-2 transfection could increase the H+ -ATPase synthesis activity of cardiomyocytes mitochondria under heat stress at 41 degrees C and 43 degrees C and could decrease the Caspase 3 activity of cardiomyocytes under heat stress at 41 degrees C and 43 degrees C.
  
  
  CONCLUSIONS
  The protection effect of bcl-2 transfection on heat-stressed cardiomyocytes may be associated with preserved H+ ATPase synthesis activity of cardiomyocytes mitochondria and the activity of Caspase 3 of cardiomyocytes.
authors.affiliation: The 148 hospital of PLA, Shandong Zibo 255300, China.
authors.forename: Xue-li
authors.initials: XL
authors.lastname: Song
authors.forename: Ling-jia
authors.initials: LJ
authors.lastname: Qian
authors.forename: Feng-zhi
authors.initials: FZ
authors.lastname: Li
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism*; D002478:Cells, Cultured; D019254:Genes, bcl-2; D018869:Heat-Shock Response / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism; D051381:Rats; D014162:Transfection
chemical_list: C505410:Casp3 protein, rat; D053148:Caspase 3; D006180:Proton-Translocating ATPases