title: Antioxidant activity of N-acyl chitosan oligosaccharide with same substituting degree.
abstract: N-Maleoyl chitosan oligosaccharide (NMCOS) and N-succinyl chitosan oligosaccharide (NSCOS) were prepared by acylation with maleic anhydride and succinic anhydride, respectively. Their structural changes were confirmed by Fourier-transform infrared (FT-IR) spectroscopy and their substituting degrees were determined both as 0.49 by conductometric titration. Their antioxidant activities were evaluated by scavenging superoxide anion O(2·)(-), hydroxyl radical ()OH and determination of reducing power. The 50% inhibition concentrations (IC(50)) of NMCOS and NSCOS scavenging effect on O(2)(·-) were 2.25 and 3.27mg/mL, respectively. The IC(50) of NMCOS scavenging effect on (·)OH was 0.24mg/mL, however, at the same concentration determined, the value of NSCOS on (·)OH was 30.5%. The reducing powers of NMCOS and NSCOS at the concentration of 2.40mg/mL were determined as 0.46 and 0.41, respectively. The above results showed that NMCOS has better antioxidant activities, which may be related to the fact that maleoyl has stronger electron-withdrawing effect than succinyl.
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China. taosun@shou.edu.cn
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Xuhong
authors.initials: X
authors.lastname: Yin
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D013481:Superoxides; D017665:Hydroxyl Radical; D048271:Chitosan