title: Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti.
abstract: Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4'-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4'-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4'-TCB as determined by gas chromatography-mass spectrometry (GC-MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tu
authors.forename: Ying
authors.initials: Y
authors.lastname: Teng
authors.forename: Yongming
authors.initials: Y
authors.lastname: Luo
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Li
authors.forename: Xianghui
authors.initials: X
authors.lastname: Sun
authors.forename: Zhengao
authors.initials: Z
authors.lastname: Li
authors.forename: Wuxing
authors.initials: W
authors.lastname: Liu
authors.forename: Peter
authors.initials: P
authors.lastname: Christie
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D003470:Culture Media; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005658:Fungi / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000378:metabolism*; D016962:Sinorhizobium meliloti / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003470:Culture Media; D004785:Environmental Pollutants; D005231:Fatty Acids, Unsaturated; D012989:Soil Pollutants; C018971:2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid; C081766:2,4,4'-trichlorobiphenyl; D011078:Polychlorinated Biphenyls