title: [Construction of tissue-engineered skin by mix-seeding].
abstract: OBJECTIVE
  To investigate the feasibility of construction of tissue-engineered skin in vitro.
  
  
  METHODS
  Fibroblasts were seeded on the dermal surface of acellular dermal matrix (ADM). 7 days later, epidermal cells (5 x 10(5)/cm2) were mixed with fibroblasts (0.2 x 10(5)/cm2) and then seeded on epidermal surface of ADM. The culture medium was the mixture liquor containing K-SFM in half and the culture supernatants of fibroblasts in half. In the control group, only epidermal cells (5 x 10(5)/cm2) were seeded and cultured with K-SFM. After composite skin was cultured for 1 week and 3 week, samples were harvested respectively for morphological study and to receive identification by immunohistochemistry.
  
  
  RESULTS
  After 3-week culture, there were 3-4 continuous layers of cells in the epidermis in the experiment group. The epidermis was attached tightly to the dermis with trochanterellus. But in the control group, there were just 1-2 layers of cells in the epidermis which was not connected to the dermis. Strong positive dye of Laminin indicated that basement membrane was thoroughly formed in the experiment group. The same result was demonstrated by transmission electron microscope.
  
  
  CONCLUSIONS
  The mix-seeding of epidermal cells and few fibroblasts would promote the adhesion and proliferation of epidermal cells on ADM, and the formation of basement membrane.
authors.affiliation: Department of Plastic Surgery, Xinhua Hospital Affiliated to Dalian University, Dalian 116021, China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Nin-Ze
authors.initials: NZ
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000166:cytology*; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds