title: Probing GFP-actin diffusion in living cells using fluorescence correlation spectroscopy.
abstract: The cytoskeleton of eukaryotic cells is continuously remodeled by polymerization and depolymerization of actin. Consequently, the relative content of polymerized filamentous actin (F-actin) and monomeric globular actin (G-actin) is subject to temporal and spatial fluctuations. Since fluorescence correlation spectroscopy (FCS) can measure the diffusion of fluorescently labeled actin it seems likely that FCS allows us to determine the dynamics and hence indirectly the structural properties of the cytoskeleton components with high spatial resolution. To this end we investigate the FCS signal of GFP-actin in living Dictyostelium discoideum cells and explore the inherent spatial and temporal signatures of the actin cytoskeleton. Using the free green fluorescent protein (GFP) as a reference, we find that actin diffusion inside cells is dominated by G-actin and slower than diffusion in diluted cell extract. The FCS signal in the dense cortical F-actin network near the cell membrane is probed using the cytoskeleton protein LIM and is found to be slower than cytosolic G-actin diffusion. Furthermore, we show that polymerization of the cytoskeleton induced by Jasplakinolide leads to a substantial decrease of G-actin diffusion. Pronounced fluctuations in the distribution of the FCS correlation curves can be induced by latrunculin, which is known to induce actin waves. Our work suggests that the FCS signal of GFP-actin in combination with scanning or spatial correlation techniques yield valuable information about the local dynamics and concomitant cytoskeletal properties.
authors.affiliation: Center for NanoScience and Fakultät für Physik, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, D-80539 München, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Engelke
authors.forename: Doris
authors.initials: D
authors.lastname: Heinrich
authors.forename: Joachim O
authors.initials: JO
authors.lastname: Rädler
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D004023:Dictyostelium; D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D015336:Molecular Probe Techniques; D013050:Spectrometry, Fluorescence
chemical_list: D000199:Actins; D049452:Green Fluorescent Proteins