title: [Zymography identification of the matrix metalloproteinases synthesized by cultured hepatic stellate cells].
abstract: OBJECTIVE
  To establish a method for identification of the activities of matrix metalloproteinases (MMPs) synthesized by cultured hepatic stellate cells (HSC).
  
  
  METHODS
  SDS-gelatin polyacrylamide gels electrophoresis was used for the identification of MMPs synthesized by cultured HSC.
  
  
  RESULTS
  MMP-2 and MMP-9 in culture medium and HSC were sensitively identified, and MMP-2 activity was higher than MMP-9 in culture medium, the changes of MMPs in culture medium and HSC were also observed after 24h medicine treatment.
  
  
  CONCLUSIONS
  Zymography is suitable for the studies on the role of HSC in the regulation of extracellular matrix turnover.
authors.affiliation: Medical Laboratory of Kunming General Hospital, Kunming 650032, China.
authors.forename: Wen-Xing
authors.initials: WX
authors.lastname: Zhao
authors.forename: Chong-Li
authors.initials: CL
authors.lastname: Liang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020782:Matrix Metalloproteinases