title: Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions.
abstract: Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters detected were affiliated to type I methanotrophs. Two novel clusters (cluster 1, cluster 2) were found to relate to none of the recognized genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and aerobic soil were different from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland.
authors.affiliation: Graduate University of Chinese Academy of Sciences, Beijing 100049, China. yunjuanli@126.com
authors.forename: Juanli
authors.initials: J
authors.lastname: Yun
authors.forename: Anzhou
authors.initials: A
authors.lastname: Ma
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Li
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongxun
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D002681:China; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008772:Methylococcaceae / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D001426:Bacterial Proteins