title: Protective effect of trifluoperazine on hydrogen peroxide-induced apoptosis in PC12 cells.
abstract: This study investigated effects of trifluoperazine (TFP) against the cytotoxicity induced by H₂O₂ in PC12 cells and the mechanisms thereof. Different concentrations of H₂O₂ (100-500 μM) induced a significant decrease in cell viability accompanied by increased oxidative stress and cell apoptosis. Pretreatment with TFP inhibited H₂O₂-induced cell viability loss. The flow cytometric assay showed that TFP can inhibit intracellular reactive oxygen species (ROS) generation and reduce the cell apoptosis. The electrophysiological recordings indicated that when treated with H₂O₂, the calcium current was significantly increased. Pretreatment with TFP increased mitochondrial membrane potential (MMP) in cells of oxidative injury. These results suggested that TFP can reduce apoptosis by inhibiting ROS generation and preventing loss of MMP in cells. Meanwhile, the protective effect of TFP on the cell apoptosis may be related to the calcium overload. TFP may inhibit the calcium overload process to achieve the protection against apoptosis.
authors.affiliation: School of Medicine, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, China.
authors.forename: Shichang
authors.initials: S
authors.lastname: Liu
authors.forename: Yangguang
authors.initials: Y
authors.lastname: Han
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015220:Calcium Channels / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D014268:Trifluoperazine / Q000494:pharmacology*
chemical_list: D015220:Calcium Channels; D016877:Oxidants; D017382:Reactive Oxygen Species; D014268:Trifluoperazine; D006861:Hydrogen Peroxide