title: Neurofilament heavy subunit in cerebrospinal fluid: a biomarker of amyotrophic lateral sclerosis?
abstract: The objectives of this study were to investigate the presence of the three neurofilament subunits, ubiquitin, proteasome and 3-nitrotyrosine, in CSF samples of ALS patients. CSF samples were obtained by lumbar puncture from 10 ALS patients and six controls. All samples were analysed by Western blotting. Results revealed that neurofilament heavy subunit was identified in 70% of ALS cases and we conclude that this subunit may be a promising biomarker for clinical diagnosis of ALS.
authors.affiliation: Departamento de Biociências, Universidade Federal de Sergipe, Brazil.
authors.forename: Deise M F
authors.initials: DM
authors.lastname: Mendonça
authors.forename: Sheila C S
authors.initials: SC
authors.lastname: Martins
authors.forename: Rafael
authors.initials: R
authors.lastname: Higashi
authors.forename: Marcelo N
authors.initials: MN
authors.lastname: Muscara
authors.forename: Vivaldo Moura
authors.initials: VM
authors.lastname: Neto
authors.forename: Leila
authors.initials: L
authors.lastname: Chimelli
authors.forename: Ana Maria B
authors.initials: AM
authors.lastname: Martinez
mesh_terms: D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid* / Q000175:diagnosis* / Q000473:pathology; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000134:cerebrospinal fluid*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D021122:Protein Subunits / Q000134:cerebrospinal fluid*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016900:Neurofilament Proteins; D021122:Protein Subunits; C002744:3-nitrotyrosine; D014443:Tyrosine