title: Quantitative analysis of Hb Bart's in cord blood by capillary electrophoresis system.
abstract: It has long been recognized that the presence of hemoglobin (Hb) Bart's in newborn's blood is associated with α-thalassemia. However, the automated high-performance liquid chromatography or low-performance liquid chromatography system is unable to quantify the amount of Hbs Bart's and H, which are eluted at the retention time close to 0 min. This study used automatic capillary electrophoresis (CE) system to diagnose various types of α-thalassemia in 587 cord blood samples, including 429 normal α-globin genotype, 120 cases of thalassemia with one α-globin gene defect, 34 cases with two α-globin genes defect, and four cases with three α-globin genes defect. The result showed that the level of Hb Bart's in cord blood was increased accordingly with the increasing numbers of the defective α-globin genes. In addition, Hb Bart's level at 0.2%, as measured by CE, can be used as a cut-off point for α-thalassemia diagnosis in newborns.
authors.affiliation: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Thailand.
authors.forename: Thongperm
authors.initials: T
authors.lastname: Munkongdee
authors.forename: Dalad
authors.initials: D
authors.lastname: Pichanun
authors.forename: Punnee
authors.initials: P
authors.lastname: Butthep
authors.forename: Sumonmaln
authors.initials: S
authors.lastname: Klamchuen
authors.forename: Veeradet
authors.initials: V
authors.lastname: Chalermpolprapa
authors.forename: Pranee
authors.initials: P
authors.lastname: Winichagoon
authors.forename: Saovaros
authors.initials: S
authors.lastname: Svasti
authors.forename: Suthat
authors.initials: S
authors.lastname: Fucharoen
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005312:Fetal Blood / Q000737:chemistry*; D005838:Genotype; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn / Q000097:blood; D010641:Phenotype; D055542:alpha-Globins / Q000032:analysis / Q000235:genetics; D017085:alpha-Thalassemia / Q000097:blood* / Q000175:diagnosis*
chemical_list: D006455:Hemoglobins, Abnormal; D055542:alpha-Globins; C030448:hemoglobin Bart's