title: Synthesis of modified siRNA bearing C-5 polyamine-substituted pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity.
abstract: Short interfering RNA (siRNA) induces specific gene silencing by the RNA interference (RNAi) pathway. Nucleosides in the 3'-overhang regions of siRNAs were replaced with 5-bis(aminoethyl)aminoethylcarbamoylmethyl-2'-deoxyuridine or thymidine. siRNA bearing modified nucleoside was more active in silencing the gene expression of hepatocyte nuclear factor 4α (HNF4α) compared with siRNA bearing thymidine.
authors.affiliation: Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, Tenjin-cho 1-5-1, Kiryu, Gunma 376-8515, Japan.
authors.forename: Mohammad Mehedi
authors.initials: MM
authors.lastname: Masud
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Masuda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Inoue
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
mesh_terms: D056945:Hep G2 Cells; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics*; D006801:Humans; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry*; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics*
chemical_list: D051557:Hepatocyte Nuclear Factor 4; D011073:Polyamines; D011741:Pyrimidine Nucleosides; D012333:RNA, Messenger; D034741:RNA, Small Interfering