title: Integrated biological responses of zebrafish (Danio rerio) to analyze water quality in regions under anthropogenic influence.
abstract: This study analyzed water quality in regions around Patos lagoon (Southern Brazil) that are under anthropogenic pressure. Water samples were collected from five different sites, including one used as a source for human consumption (COR) and others known to be influenced by human activities (IP). Danio rerio (Teleostei, Cyprinidae) organisms were exposed for 24h to these water samples, plus a control group. It was observed that: (1) reactive oxygen species levels were lower in COR and IP than in the control group; (2) glutamate-cysteine ligase (catalytic subunit) expression was higher in COR than in other sites; (3) exposure to all water samples affected long-term memory (LTM) when compared to control group. Thus, some water samples possess the ability to modulate the antioxidant system and to induce a decline in cognitive functions, as measured by LTM. The obtained results indicate that a combination of variables of different organization level (molecular, biochemical and behavioral) can be employed to analyze water quality in impacted regions.
authors.affiliation: Curso de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Universidade Federal do Rio Grande - FURG, Cx. P. 474, CEP 96.201-900, Rio Grande, RS, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Amado
authors.forename: C E
authors.initials: CE
authors.lastname: Rosa
authors.forename: M R
authors.initials: MR
authors.lastname: Castro
authors.forename: A P
authors.initials: AP
authors.lastname: Votto
authors.forename: L Cougo
authors.initials: LC
authors.lastname: Santos
authors.forename: L F F
authors.initials: LF
authors.lastname: Marins
authors.forename: G S
authors.initials: GS
authors.lastname: Trindade
authors.forename: D S
authors.initials: DS
authors.lastname: Fraga
authors.forename: R C F
authors.initials: RC
authors.lastname: Damé
authors.forename: D M
authors.initials: DM
authors.lastname: Barros
authors.forename: L A
authors.initials: LA
authors.lastname: Geracitano
authors.forename: A
authors.initials: A
authors.lastname: Bianchini
authors.forename: F R
authors.initials: FR
authors.lastname: de la Torre
authors.forename: J M
authors.initials: JM
authors.lastname: Monserrat
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*; D014881:Water Supply / Q000032:analysis; D015027:Zebrafish / Q000378:metabolism / Q000502:physiology*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione