title: [The effects of chronic hypoxic hypercapnia on the TLR4 and NFkappaB in hippocampus neuron in rats].
abstract: OBJECTIVE
  To study the expression and effect of TLR4 and NFkappaB protein in hippocampus neuron in rats exposed to chronic hypoxic hypercapnia.
  
  
  METHODS
  The disorder of learning-memory in pulmonary hypertension rat model was reproduced by chronic hypoxic hypercapnia. Thirty rats were randomly divided into three groups: normal control group, hypoxic hypercapnia 2-week and 4-week group. The number of apoptosis neurons in hippocampus CA1/3 was counted by TUNEL method. Activity of TLR4 and NFkappaB in hippocampus CA1/3 was detected by using SP immunocytochemical technique.
  
  
  RESULTS
  The expression of TLR4 protein in hippocampus CA1/3 in group 2HH( CA1: 0.1275 +/- 0.0242, CA3: 0.1156 +/- 0.0376) and 4HH (CA1: 0.1522 +/- 0.0187, CA3: 0.1427 +/- 0.0453) were significantly higher than those in the NC group (P < 0.05, P < 0.01). The positive expression of NFkappaB were showed in cell nucleus in group 2HH (CA1: 0.1326 +/- 0.0324, CA3: 0.1301 +/- 0.0112) and group 4HH (CA1: 0.1612 +/- 0.0428, CA3: 0.1578 +/- 0.0365), and significantly higher than those in the NC group (P < 0.05, P < 0.01). The apoptosis of neural cells in hippocampus CA1/3 gradually increased with the time of exposure, and reached peak at 4 weeks (P < 0.01 vs NC group).
  
  
  CONCLUSIONS
  The activation of TLR4 and NFkappaB may play an important role in the apoptosis of hippocampus neural cells in rat exposed to chronic hypoxic hypercapnia.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.forename: Sheng-Min
authors.initials: SM
authors.lastname: Shao
authors.forename: Jian-Qiong
authors.initials: JQ
authors.lastname: Ke
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Wang
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006935:Hypercapnia / Q000378:metabolism / Q000503:physiopathology*; D006976:Hypertension, Pulmonary / Q000378:metabolism / Q000503:physiopathology; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4