title: Aminoguanidine administration ameliorates hippocampal damage after middle cerebral artery occlusion in rat.
abstract: The effects of a selective inducible nitric oxide synthase inhibitor aminoguanidine (AG) on neuronal cells survival in hippocampal CA1 region after middle cerebral artery occlusion (MCAO) were examined. Transient focal cerebral ischemia was induced in rats by 60 or 90 min of MCAO, followed by 7 days of reperfusion. AG treatment (150 mg/kg i.p.) significantly reduced total infarct volumes: by 70% after 90 min MCAO and by 95% after 60 min MCAO, compared with saline-treated ischemic group. The number of degenerating neurons in hippocampal CA1 region was also markedly lower in aminoguanidine-treated ischemic groups compared to ischemic groups without AG-treatment. The number of iNOS-positive cells significantly increased in the hippocampal CA1 region of ischemic animals, whereas it was reduced in AG-treated rats. Our findings demonstrate that aminoguanidine decreases ischemic brain damage and improves neurological recovery after transient focal ischemia induced by MCAO.
authors.affiliation: Department of Neurochemistry, Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01, Košice, Slovak Republic. danielis@saske.sk
authors.forename: Viera
authors.initials: V
authors.lastname: Danielisova
authors.forename: Jozef
authors.initials: J
authors.lastname: Burda
authors.forename: Miroslava
authors.initials: M
authors.lastname: Nemethova
authors.forename: Miroslav
authors.initials: M
authors.lastname: Gottlieb
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000473:pathology*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology* / Q000503:physiopathology; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D009410:Nerve Degeneration / Q000473:pathology; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D006146:Guanidines; D052247:Nitric Oxide Synthase Type II; C004479:pimagedine