title: 5-azacytidine enhances proliferation in transplanted rat fetal epiglottis.
abstract: Fetal rat epiglottis and its developmental potential in ectopic transplants under the influence of the epigenetic drug was investigated. Epiglottises from 17-days-old rat embryos were transplanted under kidney capsules of adult rats for 14 days. 5-azacytidine (5 mg/kg) was injected intraperitoneally during first three days and controls were sham treated. TEM, immunohistochemical detection and quantitative stereological analysis of the Proliferating Cell Nuclear Antigen (PCNA) expression (numerical density N(v)) were performed. Typical chondroblasts with long surface processes and sparse lipid droplets were found in fetal epiglottis and chondrocytes with shorter processes, numerous lipid droplets and elastic fibers in adult. PCNA was expressed in almost all cells of the fetal epiglottis while in the adult it was expressed in minority of cells. In transplants, differentiation progressed towards the differentiation found in the adult. Application of 5-azacytidine increased the capacity for proliferation (N(v PCNA)) in comparison to controls but no difference in differentiation was observed. Data about the developmental potential and induction of proliferation in mammalian epiglottis by epigenetic modulation is of importance for regenerative medicine.
authors.affiliation: University Department of Dermatology and Venereology, Zagreb University Hospital Center and School of Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Marinovic-Kulisic
authors.forename: Gordana
authors.initials: G
authors.lastname: Juric-Lekic
authors.forename: Masa
authors.initials: M
authors.lastname: Vikic-Topic
authors.forename: Vedran
authors.initials: V
authors.lastname: Lokosek
authors.forename: Vedran
authors.initials: V
authors.lastname: Radujkovic
authors.forename: Floriana
authors.initials: F
authors.lastname: Bulic-Jakus
authors.forename: Ana
authors.initials: A
authors.lastname: Katusic
authors.forename: Maja
authors.initials: M
authors.lastname: Vlahovic
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Serman
authors.forename: Nino
authors.initials: N
authors.lastname: Sincic
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000008:administration & dosage / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000187:drug effects*; D004825:Epiglottis / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation*; D005333:Fetus; D006652:Histological Techniques; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D046529:Microscopy, Electron, Transmission; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D001374:Azacitidine