title: Tumor-associated glycoprotein (TAG-72) is a natural ligand for the C-type lectin-like domain that induces anti-inflammatory orientation of early pregnancy decidual CD1a+ dendritic cells.
abstract: Tumor-associated glycoprotein-72 (TAG-72) is physiologically present in secretory phase endometrium, but its presence and possible immunological role in early normal human pregnancy decidua has not received attention. The double labeling of paraffin-embedded early pregnancy decidua sections using B-72.4 anti-TAG-72 mAb and MNF 116 anti-cytokeratin mAb revealed the absence of TAG-72 in uterine decidua of normal and pathological pregnancies (non-embryonic pregnancy and missed abortion) at the implantation sites, although it was present in epithelial cells at and away from the tubal implantation site of an ectopic pregnancy. TAG-72 binds and internalizes by reacting with the mannose receptor (MR-CD206) or with DC-specific ICAM reacting non-integrin (DC-SIGN-CD209) on decidual CD1a+ cells. Decidual CD1a+ cells stimulated with TAG-72 decreased CD83 expression and diminished IL-15 and IFN-γ intracellular production. TAG-72-treated CD1a+ cells decreased IFN-γ production in syngenic decidual and allogenic cord blood T cells even in the presence of lipopolysaccharide. TAG-72- and lipopolysaccharide-pre-treated CD1a+ cells significantly increased IL-4 expression in allogenic cord blood T cells. TAG-72 increased allogenic cord blood T cell proliferation, mediated by decidual CD1a+ cells, compared with its effect on the proliferation of syngenic decidual T cells. All these data emphasize the anti-inflammatory properties of TAG-72-treated decidual CD1a+ cells in terms of their interaction with T cells. Thus, the absence of TAG-72 at the maternal-fetal interface during early pregnancy could lead to a mild pro-inflammatory response that may be beneficial for pregnancy success and trophoblast growth control.
authors.affiliation: Department of Physiology and Immunology, Medical Faculty, University of Rijeka, 51000 Rijeka, B. Branchetta 20, Croatia. gordana.laskarin@medri.hr
authors.forename: Gordana
authors.initials: G
authors.lastname: Laskarin
authors.forename: Arnela
authors.initials: A
authors.lastname: Redzovic
authors.forename: Ivan
authors.initials: I
authors.lastname: Vlastelic
authors.forename: Herman
authors.initials: H
authors.lastname: Haller
authors.forename: Suzana Srsen
authors.initials: SS
authors.lastname: Medancic
authors.forename: Graziella
authors.initials: G
authors.lastname: Solinas
authors.forename: Daniel
authors.initials: D
authors.lastname: Rukavina
mesh_terms: D015703:Antigens, CD / Q000235:genetics; D018949:Antigens, CD1 / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000378:metabolism* / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003656:Decidua / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006023:Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000378:metabolism; D019409:Interleukin-15 / Q000037:antagonists & inhibitors / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D008070:Lipopolysaccharides / Q000276:immunology; D008183:Luteal Phase; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D000097802:CD83 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D007136:Immunoglobulins; D007371:Interferon-gamma; D019409:Interleukin-15; D015847:Interleukin-4; D037181:Lectins, C-Type; D008024:Ligands; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000097802:CD83 Antigen; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C419393:CD1a antigen; C047052:tumor-associated antigen 72