title: Flavobacterium phragmitis sp. nov., an endophyte of reed (Phragmites australis).
abstract: A Gram-staining-negative bacterium, designated strain BLN2(T), was isolated from within the roots of reeds (Phragmites australis) in Beijing Cuihu Wetland (China) and characterized using a polyphasic taxonomic approach. The cells were yellow-pigmented, rod-shaped, strictly aerobic and devoid of flagella, but showed gliding motility. Strain BLN2(T) produced yellow, translucent, circular and convex colonies, with optimal growth at 30 °C and pH 7.0. The major respiratory quinone was menaquinone 6 (MK-6) and the predominant fatty acids were iso-C(15 : 0), summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), C(16 : 0) 3-OH, C(16 : 0,) iso-C(17 : 0) 3-OH and iso-C(15 : 0) 3-OH. The G+C content of the genomic DNA was 34.8 mol%. The 16S rRNA gene sequence analysis showed that strain BLN2(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium anhuiense CGMCC 1.6859(T) (97.0 % sequence similarity). The DNA-DNA relatedness between strain BLN2(T) and F. anhuiense CGMCC 1.6859(T) was 25.7 %. Based on the phenotypic data and phylogenetic inference presented, it is concluded that strain BLN2(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium phragmitis sp. nov. is proposed. The type strain is BLN2(T) ( = DSM 23314(T) = CGMCC 1.10370(T)).
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Jing Nan
authors.initials: JN
authors.lastname: Zhu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Qun Fang
authors.initials: QF
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Jin Gang
authors.initials: JG
authors.lastname: Gu
authors.affiliation: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: New Technique Centre, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Chun Li
authors.initials: CL
authors.lastname: Li
mesh_terms: D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S