title: An endocytic CARriage tale: Adenoviruses internalization and trafficking in neurons.
abstract: In immune-competent hosts, adenoviruses (Ads) are mild pathogens that cause mainly infections of the respiratory and ocular tracks. The advent of Ad-based gene transfer vectors made the understanding of their interaction with the host cellular machinery an intensive field of research over the last decade. As studies focused primarily on epithelial-like cells, the mechanism of neuronal uptake of Ads was still missing. Using a combination of biochemical and cell biology approaches, we characterized the axonal trafficking pathway used by the canine adenovirus serotype 2 (CAV-2) to reach the neuronal soma. We showed that CAV-2 and CAR (coxsackievirus and adenovirus receptor) are entering a vesicular pathway coupled to the axonal transport machinery. The lumen of the multivalent Rab7 (+) vesicles that transport CAV-2 and CAR is, surprisingly, pH neutral. Among other issues, our study opens numerous questions concerning the neuronal function of CAR.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535, Montpellier, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Henaff
authors.forename: Sara
authors.initials: S
authors.lastname: Salinas
mesh_terms: D006516:Adenoviruses, Canine / Q000378:metabolism / Q000472:pathogenicity*; D000818:Animals; D001370:Axonal Transport; D001369:Axons; D002478:Cells, Cultured; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D004285:Dogs; D004705:Endocytosis; D011992:Endosomes / Q000821:virology*; D051379:Mice; D009046:Motor Neurons / Q000821:virology*; D011991:Receptors, Virus / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus