title: [Studies of protective effect of SOD on damage of human RBCs stored at 4 degrees C].
abstract: OBJECTIVE
  To investigate the mechanism of protective effect of SOD (superoxide dismutase) on damage of RBCs stored at 4 degrees C, the studies of erythrocyte glucose and energy metabolism were performed.
  
  
  METHODS
  whole blood collected from healthy donors and stored at 4 degrees C in ACD, GMA and SOD solutions. Before and post storage, some parameters were assayed. Standard methods were used for the in vitro tests. The 24-hour in vivo recoveries were measured by FTTC (Fluorescein 5-isothiocyanate) from SIGMA Company.
  
  
  RESULTS
  All parameters of red blood cell glucolysis rate without oxygen condition, ATP, PK (pyruvic kinase) and 24 h recoveries level were 86.2%, 56.4%, 64.3% and 86.2% of normal respectively stored in SOD solution at 4 degrees C for 75 days, distinctly more than in ACD and GMA groups at 75 days stored. The 24 h recovery at 75d in group SOD was near the recovery at 42d in group GMA.
  
  
  CONCLUSIONS
  Whole blood in SOD solution can be stored satisfactorily for 75 days at 4 degrees C, and furnished theoretical evidence for RBCs survival.
authors.affiliation: Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: En-Pu
authors.initials: EP
authors.lastname: Ma
authors.forename: Xiu-Zhen
authors.initials: XZ
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Ren
authors.forename: An
authors.initials: A
authors.lastname: Lin
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Xing
authors.initials: X
authors.lastname: Lü
authors.forename: Zu-Ze
authors.initials: ZZ
authors.lastname: Wu
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D011817:Rabbits; D012034:Refrigeration; D013482:Superoxide Dismutase / Q000494:pharmacology*
chemical_list: D013482:Superoxide Dismutase