title: Neuronal expression of inducible nitric oxide synthase in hypothyroid rat.
abstract: OBJECTIVE
  The expression of iNOS mRNA and protein levels was studied in hypothyroid rat cerebral cortex during early days after postnatal life.
  
  
  METHODS
  The homogenate of the cerebral cortex was examined using Real-time reverse transcription polymerase chain reaction (real-time RT PCR) and Western blot analysis.
  
  
  RESULTS
  ΔCt in hypothyroid group on day 7, 14, 21 was 18.89±0.92, 18.83±0.99, 16.48±0.29, separately. While in control group, ΔCt was 20.32±0.92, 20.07±0.86, 17.96±0.50, separately. Meanwhile, the protein level was detected only in hypothyroid group of 59 ± 5% on day 14. On day 21, iNOS protein expression showed a decrease (p<0.05) in hypothyroid group (37 ± 3%), compared with control group (58 ± 4%).
  
  
  CONCLUSIONS
  Our findings show the successive changes of the iNOS mRNA and protein levels in early postnatal days of hypothyroid rat brain, and confirm the cross-talk between the TH and NO signaling pathway in developing cerebral cortex of rats.
authors.affiliation: Department of Medicine, The Children's Hospital of Zhejiang University School of Medicine, China.
authors.forename: Jialu
authors.initials: J
authors.lastname: Xu
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.forename: Chaochun
authors.initials: C
authors.lastname: Zou
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D007037:Hypothyroidism / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat