title: [Adaptation of myofibrilla, MHC and metabolic enzyme of rabbit diaphragm muscle to different frequency chronic electrical stimulation].
abstract: OBJECTIVE
  To detect effect of the different frequency of chronic electrical stimulation (CES) on myofibrillar isoform, myosin heavy chain (MHC) and metabolic enzyme activities.
  
  
  METHODS
  The histochemical method and SDS-polyacrylamide gel electrophoresis were respectively employed.
  
  
  RESULTS
  (1)There were a significant increase in I myo-fibrillar isoform and I MHC isoform and decrease in II B myofibrillar isoform and II B MHC isoforms in the chronic low frequency electrical stimulation (CLFES) 10 Hz and 20 Hz groups, but opposite results were found in the chronic high frequency electrical stimulation (CHFES) 50 Hz and 100 Hz groups. (2) There were a significant increase in the aerobic-oxidative enzyme activities and capacity, and a concomitant significant drop in glycolysis enzyme activities in CLFES groups, but opposite results were found in CHFES 50 Hz and 100 Hz groups.
  
  
  CONCLUSIONS
  It was suggested that there was a significant dependent relation between chronic electrical stimulation frequency and myofibrilla isoforms, myosin heavy chain (MHC) and metabolic enzyme activities.
authors.affiliation: Department of Respiratory, Second Artillerymen General Hospital, Beijing 100088.
authors.forename: Sui-Yang
authors.initials: SY
authors.lastname: Zhang
authors.forename: Fang-Mei
authors.initials: FM
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Dong-Lin
authors.initials: DL
authors.lastname: Wang
authors.forename: Xian-Jian
authors.initials: XJ
authors.lastname: Guo
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Qian
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D003964:Diaphragm / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology*; D004558:Electric Stimulation; D009119:Muscle Contraction; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB / Q000378:metabolism*; D020033:Protein Isoforms; D011817:Rabbits
chemical_list: D020033:Protein Isoforms; D024501:Nonmuscle Myosin Type IIB; C103815:nonmuscle myosin type IIB heavy chain; D018995:Myosin Heavy Chains