title: Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques.
abstract: Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly-ɛ-caprolactone scaffolds are visualized by two-photon excitation microscopy (TPEM) and second-harmonic generation (SHG) imaging in both forward and backward nondescanned modes. Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220 Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Filova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Burdikova
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichova
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianchini
authors.forename: Martin
authors.initials: M
authors.lastname: Capek
authors.forename: Eva
authors.initials: E
authors.lastname: Kostakova
authors.forename: Evzen
authors.initials: E
authors.lastname: Amler
authors.forename: Lucie
authors.initials: L
authors.lastname: Kubinova
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation*; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D017711:Nonlinear Dynamics; D011817:Rabbits; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen