title: Local antibiotic delivery with demineralized bone matrix.
abstract: A method of care for these infected nonunions is prolonged intravenous systemic antibiotic treatment and implantation of methyl methacrylate antibiotic carrier beads to delivery high local doses of antibiotics. This method requires a second surgery to remove the beads once the infection has cleared. Recent studies have investigated the use of biodegradable materials that have been impregnated with antibiotics as tools to treat bone infections. In the present study, human demineralized bone matrix (DBM) was investigated for its ability to be loaded with an antibiotic. The data presented herein demonstrates that this osteoinductive and biodegradable material can be loaded with gentamicin and release clinically relevant levels of the drug for at least 13 days in vitro. This study also demonstrates that the antibiotic loaded onto the graft has no adverse effects on the osteoinductive nature of the DBM as measured in vitro and in vivo. This bone void filler may represent a promising option for local antibiotic delivery in orthopedic applications.
authors.affiliation: Research and Development Group, RTI Biologics, 11621 Research Circle, Alachua, FL 32616, USA. clewis@rtix.com
authors.forename: Christine S
authors.initials: CS
authors.lastname: Lewis
authors.forename: Peter R
authors.initials: PR
authors.lastname: Supronowicz
authors.forename: Rasa M
authors.initials: RM
authors.lastname: Zhukauskas
authors.forename: Elise
authors.initials: E
authors.lastname: Gill
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Cobb
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D016348:Osseointegration / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D000469:Alkaline Phosphatase