title: Engineered cartilage covered ear implants for auricular cartilage reconstruction.
abstract: Cartilage tissues are often required for auricular tissue reconstruction. Currently, alloplastic ear-shaped medical implants composed of silicon and polyethylene are being used clinically. However, the use of these implants is often associated with complications, including inflammation, infection, erosion, and dislodgement. To overcome these limitations, we propose a system in which tissue-engineered cartilage serves as a shell that entirely covers the alloplastic implants. This study investigated whether cartilage tissue, engineered with chondrocytes and a fibrin hydrogel, would provide adequate coverage of a commercially used medical implant. To demonstrate the in vivo stability of cell-fibrin constructs, we tested variations of fibrinogen and thrombin concentration as well as cell density. After implantation, the retrieved engineered cartilage tissue was evaluated by histo- and immunohistochemical, biochemical, and mechanical analyses. Histomorphological evaluations consistently showed cartilage formation over the medical implants with the maintenance of dimensional stability. An initial cell density was determined that is critical for the production of matrix components such as glycosaminoglycans (GAG), elastin, type II collagen, and for mechanical strength. This study shows that engineered cartilage tissues are able to serve as a shell that entirely covers the medical implant, which may minimize the morbidity associated with implant dislodgement.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Winston-Salem, North Carolina 27157, USA.
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Christopher
authors.initials: C
authors.lastname: Broda
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
authors.forename: James J
authors.initials: JJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D019902:Chondrocytes / Q000737:chemistry / Q000166:cytology; D004425:Ear Cartilage / Q000737:chemistry* / Q000166:cytology; D005340:Fibrinogen / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D008422:Materials Testing; D019736:Prostheses and Implants; D011817:Rabbits; D013917:Thrombin / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D005340:Fibrinogen; D013917:Thrombin