title: [The effects of Sphing-1-phosphate(S1P) on the potassium channel of the ventricular myocytes].
abstract: OBJECTIVE
  To study the effect of Sphingosine-1-phosphate on the delayed rectifier potassium current (IK) and the inward rectifier potassium current (IK1) of guinea pig isolated ventricular myocytes.
  
  
  METHODS
  Whole cell patch clamp technique was applied to record the delayed rectifier potassium current and the delayed rectifier potassium current of guinea pig isolated ventricular myocytes.
  
  
  RESULTS
  (1) IK of S1P (1.1 micromol/L) decreased from (1.2 +/- 0.26)nA to (0.95 +/- 0.23)nA. While IK of S1P (2.2 micromol/L) decreased from (1.43 +/- 0.31)nA to (1.02 +/- 0.28)nA. There was significant difference compared to control group (P < 0.01, n = 6). The IK peak value was decreased from (1.29 +/- 0.26) nA to (1.26 +/- 0.37)nA at the group of S1P (1.1 micromol/L) plus suramin (200 micromol/L) and showed no significant difference compared to control group (P > 0.05, n = 6). (2) IK1 of S1P (1.1 micromol/L) decreased from (-8.94 +/- 2.01)nA to (-8.81 = 1.55)nA. While IK of S1P (2.2 micromol/L) decreased from (-8.86 +/- 1.59)nA to (-8.55 +/- 1.39)nA. There was no significant difference compared to control group (P > 0.05, n = 6).
  
  
  CONCLUSIONS
  S1P inhibits delayed rectifier potassium current of ventricular myocyte in guinea pig remarkably, S1P shows no effects on delayed rectifier potassium current of ventricular myocyte in guinea pig.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Innemongol Nation University, Tongliao 028000.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D051668:Delayed Rectifier Potassium Channels / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology* / Q000378:metabolism; D008246:Lysophospholipids / Q000494:pharmacology*; D008297:Male; D032383:Myocytes, Cardiac / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects*; D024661:Potassium Channels, Inwardly Rectifying / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D051668:Delayed Rectifier Potassium Channels; D008246:Lysophospholipids; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; C060506:sphingosine 1-phosphate; D013110:Sphingosine