title: Expression of S100A8/A9 in HaCaT keratinocytes alters the rate of cell proliferation and differentiation.
abstract: S100A8/A9 promotes NADPH oxidase in HaCaT keratinocytes and subsequently increases NFκB activation, which plays important roles in the balance between epidermal growth and differentiation. S100A8/A9-positive HaCaT cells present with a significantly reduced rate of cell division and greater expression of two keratinocyte differentiation markers, involucrin and filaggrin, than control cells. S100A8/A9 mutants fail to enhance NFκB activation, TNFα-induced IL-8 gene expression and NFκB p65 phosphorylation, and S100A8/A9-positive cells demonstrate better cell survival in forced suspension culture than mutant cells. S100A8/A9 is induced in epithelial cells in response to stress. Therefore, S100A8/A9-mediated growth arrest could have implications for tissue remodeling and repair.
authors.affiliation: Institute of Immunology, University of Muenster, Muenster, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Voss
authors.forename: Günther
authors.initials: G
authors.lastname: Bode
authors.forename: Claudia
authors.initials: C
authors.lastname: Sopalla
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Benedyk
authors.forename: Georg
authors.initials: G
authors.lastname: Varga
authors.forename: Markus
authors.initials: M
authors.lastname: Böhm
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Nacken
authors.forename: Claus
authors.initials: C
authors.lastname: Kerkhoff
mesh_terms: D000222:Adaptation, Physiological; D040501:Calgranulin A / Q000235:genetics*; D040502:Calgranulin B / Q000235:genetics*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology; D000091344:Filaggrin Proteins; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism
chemical_list: D040501:Calgranulin A; D040502:Calgranulin B; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D016328:NF-kappa B