title: [Effect of gap junction on the cardioprotection of ischemic postconditioning in rat heart].
abstract: OBJECTIVE
  To determine whether the cardioprotection of ischemic postconditioning and heptanol in ischemic heart against ischemia/reperfusion (I/R) is mediated by gap junction.
  
  
  METHODS
  The effect of ischemic postconditioning, heptanol at different doses (0.03, 0.06, 0.30, and 0.60 mg/kg) and AAP10 (10 mg/kg) on the intact rat heart during 30 min ischemia and 2 h of reperfusion was observed. Ischemic postconditioning was achieved by 3 cycles of 10 s reperfusion/10 s regional ischemia starting at the beginning of the reperfusion. The infarct size and the arrhythmia scores were measured. The effect of ischemic postconditioning, heptanol at different doses (0.05, 0.10, 0.50 and 1.00 mmol/L) and AAP10 (1 x 10(-7)mol/L) on the isolated heart during 30 min ischemia and 2 h of reperfusion was observed. Ischemic postconditioning was achieved by 6 cycles of 10 s reperfusion/10 s global ischemia starting at the beginning of the reperfusion. The arrhythmia scores and conduction velocity of ventricle muscle were measured.
  
  
  RESULTS
  In the intact rat heart model, ischemic postconditioning and heptanol reduced infarct size and arrhythmia scores. In the Langendorff perfused rat heart model, ischemic postconditioning and heptanol reduced arrhythmia scores and conduction velocity of ventricle muscle. Administration of AAP10, an opener of gap junction attenuated the cardioprotection of ischemic postconditioning and heptanol.
  
  
  CONCLUSIONS
  The cardioprotection of ischemic postconditioning and heptanol may be related to the attenuation of gap junction communication on myocardiac ischemia/reperfusion injury.
authors.affiliation: Department of Physiology, Shaoxing University School of Medicine, Shaoxing 312000, China.
authors.forename: Hong-Jiao
authors.initials: HJ
authors.lastname: Mao
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Chen
authors.forename: Tu-Nan
authors.initials: TN
authors.lastname: Yu
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Ye
authors.forename: Xiang-Gui
authors.initials: XG
authors.lastname: Yuan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D017629:Gap Junctions / Q000502:physiology*; D019850:Heptanol / Q000494:pharmacology; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019850:Heptanol