title: Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58.
abstract: We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.
authors.affiliation: oschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic.
authors.forename: Khomaini
authors.initials: K
authors.lastname: Hasan
authors.forename: Andrea
authors.initials: A
authors.lastname: Fortova
authors.forename: Tana
authors.initials: T
authors.lastname: Koudelakova
authors.forename: Radka
authors.initials: R
authors.lastname: Chaloupkova
authors.forename: Mayuko
authors.initials: M
authors.lastname: Ishitsuka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nagata
authors.forename: Jiri
authors.initials: J
authors.lastname: Damborsky
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Prokop
mesh_terms: D016960:Agrobacterium tumefaciens / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D000473:Alkanes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004952:Esters / Q000378:metabolism; D006867:Hydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology; D055503:Protein Multimerization; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D000473:Alkanes; D004269:DNA, Bacterial; D004952:Esters; D006867:Hydrolases; C046137:haloalkane dehalogenase