title: Serum amyloid A induces reactive oxygen species (ROS) production and proliferation of fibroblast.
abstract: Serum amyloid A (SAA) levels are elevated highly in acute phase response and elevated slightly and persistently in chronic diseases such as rheumatoid arthritis and diabetes. Given that fibroblasts exert profound effects on progression of inflammatory chronic diseases, the aim of this study was to investigate the response of fibroblasts to SAA. A dose-dependent increase in O(2) (-) levels was observed by treatment of fibroblasts with SAA (r = 0·99 and P ≤ 0·001). In addition, the expression of p47-phox was up-regulated by SAA (P < 0·001) and diphenyliodonium (DPI), a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, reduced the release of O(2) (-) by 50%. Also, SAA raised fibroblast proliferation (P < 0·001) and this effect was completely abolished by the addition of anti-oxidants (P < 0·001). These findings support the notion that, in chronic inflammatory sites, SAA activated fibroblast proliferation and ROS production.
authors.affiliation: Instituto de Ciências da Atividade Física e Esportes, Universidade Cruzeiro do Sul, São Paulo, Brazil. ehata@usp.br
authors.forename: E
authors.initials: E
authors.lastname: Hatanaka
authors.forename: A
authors.initials: A
authors.lastname: Dermargos
authors.forename: H A
authors.initials: HA
authors.lastname: Armelin
authors.forename: R
authors.initials: R
authors.lastname: Curi
authors.forename: A
authors.initials: A
authors.lastname: Campa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D051379:Mice; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000235:genetics; D009861:Onium Compounds / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000685:Serum Amyloid A Protein / Q000494:pharmacology*; D041701:Swiss 3T3 Cells
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D009861:Onium Compounds; D017382:Reactive Oxygen Species; D000685:Serum Amyloid A Protein; C031291:diphenyliodonium; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1