title: High-resolution echocardiographic assessment of infarct size and cardiac function in mice with myocardial infarction.
abstract: BACKGROUND
  The aim of this study was to develop a simple and reasonably precise echocardiographic method for the assessment of infarct size (IS) and cardiac dysfunction in mice after myocardial infarction.
  
  
  METHODS
  In vivo experiments were performed in C57BL/6J wild-type mice (n = 18) before and 48 hours after left anterior descending coronary artery ligation. Endocardial length-based echocardiographic IS was validated with that by three different histologic measurements. Left ventricular function was evaluated.
  
  
  RESULTS
  Excellent agreement was found between endocardial length-based echocardiographic measurement and angle-based histologic measurement of IS (r = 0.82, P < .001), and both methods strongly correlated with Tei index (r = 0.82, P < .001, and r = 0.74, P < .01) and fractional area change (r = -0.61, P < .05, and r = -0.81, P < .001).
  
  
  CONCLUSIONS
  Endocardial length-based echocardiographic measurement proved to be a useful method for assessing myocardial IS and is applicable for biomedical and imaging research, and appears particularly promising in studies of left ventricular remodeling after myocardial infarction.
authors.affiliation: University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Yuan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Mark L
authors.initials: ML
authors.lastname: Kahn
authors.forename: Victor A
authors.initials: VA
authors.lastname: Ferrari
mesh_terms: D000465:Algorithms; D000818:Animals; D005260:Female; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D051379:Mice; D009203:Myocardial Infarction / Q000150:complications / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014463:Ultrasonography; D018487:Ventricular Dysfunction, Left / Q000000981:diagnostic imaging* / Q000209:etiology / Q000503:physiopathology*