title: Effect of seed sludge on characteristics and microbial community of aerobic granular sludge.
abstract: Aerobic granular sludge was cultivated by using different kinds of seed sludge in sequencing batch airlift reactor. The influence of seed sludge on physical and chemical properties of granular sludge was studied; the microbial community structure was probed by using scanning electron microscope and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that seed sludge played an important role on the formation of aerobic granules. Seed sludge taken from beer wastewater treatment plant (inoculum A) was more suitable for cultivating aerobic granules than that of sludge from municipal wastewater treatment plant (inoculum B). Cultivated with inoculum A, large amount of mature granules formed after 35 days operation, its SVI reached 32.75 mL/g, and SOUR of granular sludge was beyond 1.10 mg/(g x min). By contrast, it needed 56 days obtaining mature granules using inoculum B. DGGE profiles indicated that the dominant microbial species in mature granules were 18 and 11 OTU when inoculum A and B were respectively employed as seed sludge. The sequencing results suggested that dominant species in mature granules cultivated by inoculum A were Paracoccus sp., Devosia hwasunensi, Pseudoxanthomonas sp., while the dominant species were Lactococcus raffinolactis and Pseudomonas sp. in granules developed from inoculum B.
authors.affiliation: School of Resource and Environmental Engineering, Heilongjiang Institute of Science and Technology, Harbin 150027, China. szwcyp@tom.com
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Song
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Pan
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis; D058645:Denaturing Gradient Gel Electrophoresis; D008855:Microscopy, Electron, Scanning; D016133:Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
chemical_list: D012722:Sewage