title: Determination of the L-enantiomer of nateglinide in pharmaceutical formulations by micellar electrokinetic chromatography.
abstract: An analytical micellar electrokinetic chromatographic method was developed and validated for the determination of the L-enantiomer of nateglinide. Separations were carried out in a 50 μm, 64.5/56 fused-silica capillary. The optimized conditions included 75 mM borate buffer, pH 9.2, containing 50 mM of sodium dodecyl sulfate and 25 mg/mL of methyl-β-cyclodextrin as background electrolyte, an applied voltage of 20 kV and a temperature of 15, UV detector at 210 nm. The assay was validated for the L-enantiomer of nateglinide. The limit of detection and quantification were 0.07 and 0.2% respectively. Intraday precision was ranged between 0.12 and 1.7%. Interday precision ranged between 0.73 and 1.73%. The assay was applied to the determination of the L-enantiomer of nateglinide in pharmaceutical formulations.
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea.
authors.forename: Thanh Dung
authors.initials: TD
authors.lastname: Phan
authors.forename: Ngoc Hoan
authors.initials: NH
authors.lastname: Nguyen
authors.forename: Dae Joong
authors.initials: DJ
authors.lastname: Kim
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Lee
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Kyeong Ho
authors.initials: KH
authors.lastname: Kim
mesh_terms: D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000032:analysis*; D008823:Micelles; D000077715:Nateglinide; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D002021:Buffers; D003510:Cyclohexanes; D007004:Hypoglycemic Agents; D008823:Micelles; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D012967:Sodium Dodecyl Sulfate; D000077715:Nateglinide; D010649:Phenylalanine