title: Comparison of the QIAsymphony automated nucleic acid extraction and PCR setup platforms with NucliSens easyMAG and Corbett CAS-1200 liquid handling station for the detection of enteric pathogens in fecal samples.
abstract: In this study we compared the QIAsymphony Sample Preparation and Assay Setup modules (Qiagen), which provide automated nucleic acid extraction and PCR setup, to the NucliSens easyMAG (bioMérieux) and CAS-1200 liquid handling station (Corbett) for a molecular screening approach of enteric pathogens in fecal samples using multiplex real-time PCR. The relative DNA recovery of both platforms, within- and between-run reproducibility and a prospective study, including 510 clinical fecal samples, were performed. The results demonstrated that the QIAsymphony Sample Preparation and Assay Setup modules were highly reproducible and achieve equal performance, quantitative and qualitative, when compared with the NucliSens easyMAG and CAS-1200 systems for the molecular screening analysis of enteric pathogens in fecal samples.
authors.affiliation: Laboratory for Infectious Diseases, Department of Research and Development, Groningen, The Netherlands. e.van.zanten@infectielab.nl
authors.forename: E
authors.initials: E
authors.lastname: van Zanten
authors.forename: G J
authors.initials: GJ
authors.lastname: Wisselink
authors.forename: W
authors.initials: W
authors.lastname: de Boer
authors.forename: S
authors.initials: S
authors.lastname: Stoll
authors.forename: R
authors.initials: R
authors.lastname: Alvarez
authors.forename: A M D
authors.initials: AM
authors.lastname: Kooistra-Smid
mesh_terms: D001331:Automation / Q000379:methods; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008403:Mass Screening / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic