title: Variation of human papillomavirus 16 in cervical and lung cancers in Sichuan, China.
abstract: Although the crucial role of human papillomaviruses (HPVs), especially HPV-16 in various cancers has been confirmed, the variation of HPV-16 among different cancers have not been investigated in a specific geographic location. In order to elucidate whether similar HPV-16 variants are involved in different kinds of cancers in the same geographic location, the analysis of sequence variants of E6 and E7 oncogenes and L1 gene of HPV-16 in cervical and lung cancers in Sichuan, China, was carried out. Tissue samples from 122 cervical cancers, 104 lung cancers, and 138 controls were subjected to RT-PCR or PCR, sequencing, and sequence analysis. The infection rates of HPV-16 in cervical, lung cancers, and non-malignant controls were 68.9%, 17.3%, and 37.0%, respectively. Asian prototype variants prevailed in cervical and lung cancers, while European prototype variants in non-malignant controls. In comparison to the lung cancer, cervical cancer showed a much higher diversity of HPV-16 oncogenes. These results indicate that in Sichuan, China, Asian prototype variants of HPV-16 are more pathogenic than their European counterparts.
authors.affiliation: Sichuan University, Sichuan, People's Republic of China. qmrjzz@126.com
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Han
authors.forename: Z H
authors.initials: ZH
authors.lastname: Yang
authors.forename: L X
authors.initials: LX
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: H Z
authors.initials: HZ
authors.lastname: Hu
authors.forename: M R
authors.initials: MR
authors.lastname: Xi
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D052162:Human papillomavirus 16 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D055815:Young Adult
chemical_list: D009856:Oncogene Proteins, Viral