title: Analysis of AchE and LDH in mollusc, Lamellidens marginalis after exposure to chlorpyrifos.
abstract: The enzymes Acetylcholinesterase (AchE) and Lactatedehydrogenase (LDH) are used as biological markers in the present study. Enzymes are highly sensitive and used to evaluate the biological effects of organophosphate pesticide chlorpyrifos in freshwater mussel Lamellidens marginalis. The test organisms were exposed to sub-lethal concentration (5 ppm) of chlorpyrifos for 30 days and allowed to recover for seven days. A distinct reduction of the enzyme AchE (34 +/- 3.3 U l(-1)) was found in the treated hepatopancreas. A significant increase in LDH activity in gill, hepatopancreas and muscle was observed. There was a significant recovery in AchE and LDH in the different tissues, after seven days recovery period.. Hence, the changes in the enzymes are found as the best biomarkering tool to evaluate the effect of organophosphate pesticide chlorpyrifos on the aquatic biota.
authors.affiliation: P.G. Department of Zoology, Jamal Mohamed College, Tiruchirappalli - 620 020, India. drba_zo@yahoo.com
authors.forename: B
authors.initials: B
authors.lastname: Amanullah
authors.forename: A
authors.initials: A
authors.lastname: Stalin
authors.forename: P
authors.initials: P
authors.lastname: Prabu
authors.forename: S
authors.initials: S
authors.lastname: Dhanapal
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004390:Chlorpyrifos / Q000633:toxicity*; D043143:Hepatopancreas / Q000187:drug effects / Q000201:enzymology; D007306:Insecticides / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008974:Mollusca / Q000187:drug effects* / Q000201:enzymology
chemical_list: D007306:Insecticides; D007770:L-Lactate Dehydrogenase; D000110:Acetylcholinesterase; D004390:Chlorpyrifos