title: Isolation and detection of protein with nano-particles and microchips for analyzing proteomes on a large scale basis.
abstract: The advent of sugar-immobilized gold nano-particles (SGNPs), lipid-based nanoparticles, nano-chromatography and nano-electrophoresis has revolutionized the methodology for protein purification and proteomic research. This review provides an overview on the effective method developed for fast purification of protein from extracts using SGNPs. In addition, the current application of microfluidic systems for analytical purposes in biochemistry will also be explored that include the micro total analysis systems (µ-TAS) and lab-on-a-chip (LOC) analyses which are capable of isolation and detection of protein at the nanogram level. Finally, we describe why the lipid-based nano-particles (LBNPs) can enable the analysis in microchip electroseparation and how anionic and cationic LBNPs can be used for protein separation.
authors.affiliation: Biomedical and Tissue Engineering Research Group, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, China. b021770@mailserv.cuhk.edu.hk
authors.forename: Ki
authors.initials: K
authors.lastname: Chan
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D053801:Capillary Electrochromatography; D006046:Gold / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D056656:Lab-On-A-Chip Devices; D008055:Lipids / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D020543:Proteome / Q000032:analysis*
chemical_list: D008055:Lipids; D011506:Proteins; D020543:Proteome; D006046:Gold