title: Immunohistochemistry expression of tumor markers CD34 and P27 as a prognostic factor of clinically localized prostate adenocarcinoma after radical prostatectomy.
abstract: OBJECTIVE
  to analyze the immunohistochemical expression of P27 and CD34 markers as prognostic factors in patients with localized prostate cancer.
  
  
  METHODS
  analysis of 100 patients with localized prostate cancer submitted to curative surgery. We carried out the usual histological preparation, followed by immunohistochemistry to detect the accumulation of P27 and CD34 protein followed by statistical analysis.
  
  
  RESULTS
  in the evaluation of P27 marker and on the correlation with the variables we found significant difference in Gleason score with positive expression (positive P27) related to lower mean PSA (p = 0.091), lower Gleason score (p < 0.0001) and smaller tumor area in CD34 (p = 0.036). Regarding the CD34 marker at the tumor area, it was observed that the smaller the positive CD34, the lower the PSA value (p < 0.0001) and lower the Gleason score (r = 0.5726, p < 0.0001), and the higher the positive CD34, the higher the staging (r = 0.3305, p <0.0001) and the chance of recurrence (p = 0.002). Patients with higher stage also displayed larger positive CD34 areas (p < 0.0001).
  
  
  CONCLUSIONS
  the markers CD34 and P27 are associated with events specific to prostate cancer, however, only CD34 was able to determine the possibility of biochemical recurrence.
authors.affiliation: Graduate Program in Principles of Surgery, Hospital de Clínicas/UFPR, Curitiba, PR, Brazil. aenassif@gmail.com
authors.forename: Aissar Eduardo
authors.initials: AE
authors.lastname: Nassif
authors.forename: Renato
authors.initials: R
authors.lastname: Tâmbara Filho
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism* / Q000601:surgery*; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000096:biosynthesis*; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000601:surgery*
chemical_list: D018952:Antigens, CD34; D018809:Proliferating Cell Nuclear Antigen; C413374:p27 antigen