title: Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes.
abstract: Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold higher than the control transformants harboring the wild-type phaB gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the phaC gene exhibited no apparent effect on P(3HB) production. This result suggests that the efficiency of PhaB-catalyzed reaction contributed to the flux toward P(3HB) biosynthesis in tobacco leaves.
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Hokkaido University, N13-W8 Kita-ku, Sapporo 060-8628, Japan. mken@eng.hokudai.ac.jp
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Morimoto
authors.forename: Aoi
authors.initials: A
authors.lastname: Gohda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D003062:Codon / Q000378:metabolism; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D006885:Hydroxybutyrates / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism; D014176:Protein Biosynthesis; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon; D006885:Hydroxybutyrates; D011091:Polyesters; D000429:Alcohol Oxidoreductases; C023744:acetoacetyl-CoA reductase; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase