title: [Nongenomic action of corticosterone to calcium rise induced by high-K+ in PC12 cells].
abstract: OBJECTIVE
  To analyse the mechanism of corticosterone on the elevation of cytosolic free calcium ([Ca2+]i) induced by high-K+ in pheochromocytoma PC12 cells,
  
  
  METHODS
  The [Ca2+]i was real-time checked by fluorescence image system.
  
  
  RESULTS
  (1) When the cells were preincubated at 37 degrees C for 5 min in the presence of various concentration corticosterone and stimulated with 55 mmol/L KCl , an inhibitory effect of corticosterone on delta[Ca2+]i was observed in a concentration-dependent manner. (2) When PC12 cells were preincubated with various concentration of B-BSA at 37 degrees C for 5 min and stimulated with 55 mmol/L KCl, an inhibitory effect of B-BSA on delta[Ca2+]i was observed, which is also concentration-dependent manner. (3) The inhibitory effect of corticosterone and B-BSA could not be antagonized by RU38486 at 10(-4) mol/L. (4) cycloheximide could not block the inhibitory effect of corticosterone after pretreating cells at 10(-5) mol/L at 37 degrees C for 3 hours.
  
  
  CONCLUSIONS
  It is obvious that the locus of corticosterone action is on the plasmic membrane. The inhibitory effect of corticosterone is independent of protein synthesis and intracellular glucocorticoid receptor. The effect of corticosterone on [Ca2+]i is nongenomic action in PC12 cells.
authors.affiliation: Department of Neurobiology, Institute of Neuroscience, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Lou
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Huang
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Han
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Yi-Zhang
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003345:Corticosterone / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D051381:Rats
chemical_list: D011188:Potassium; D002118:Calcium; D003345:Corticosterone