title: Assessment of Dietzia subsp. C79793-74 for treatment of cattle with evidence of paratuberculosis.
abstract: The objective of the present investigation was to determine whether the bacterium Dietzia subsp. C79793-74, previously shown to inhibit growth of Mycobacterium subsp. paratuberculosis under in vitro culture conditions, has therapeutic value as a probiotic for adult cattle with paratuberculosis.  Animals were obtained from several herds with evidence of disease based on seropositivity and/or fecal shedding.  Sixty-eight cows with initial evidence of Stage II or III paratuberculosis and 2 with an initial Stage IV disease were evaluated longitudinally.  Animals were either treated daily with variable, disease-dependent doses of Dietzia (n = 48) or left untreated (n = 22).  Clinical aspects of disease (diarrhea, emaciated, cachectic and appetite) were recorded until the animal recovered or required euthanasia due to advanced clinical paratuberculosis or other severe conditions.  Paratuberculosis parameters-antibody serology (ELISA, AGID) and fecal culture-were longitudinally monitored over the lifetime of each animal.  The results indicated that daily treatment with Dietzia was therapeutic for paratuberculosis cows based on: (a) longitudinal decline in ELISA values only occurred in animals that were treated; (b) prolonged survival was dependant upon treatment--the length being directly associated with low initial ELISA values; and (c) treated animals were the only ones cured of disease.  Further investigations are envisaged to determine optimal, long-term dosages that may result in even better therapeutic outcomes as well as to evaluate potential application for therapy of the Johne's disease, human-counterpart, Crohn's disease.
authors.affiliation: University of Wisconsin-River Falls, River Falls, WI, USA. mbclicker@msn.com
authors.forename: Robert E
authors.initials: RE
authors.lastname: Click
authors.forename: Craig L
authors.initials: CL
authors.lastname: Van Kampen
mesh_terms: D000192:Actinomycetales / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology / Q000628:therapy*; D015169:Colony Count, Microbial / Q000379:methods / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology / Q000628:therapy*; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D000907:Antibodies, Bacterial