title: [The influence of dehydrocorydaline on intracellular free calcium concentration during hypoxia in myocardial cell of guinea-pigs].
abstract: OBJECTIVE
  To study the effect of Dehydrocorydaline and Verapamil (Ver) on intracellular free calcium concentration of myocardial cell ([Ca2+]i) under hypoxic condition.
  
  
  METHODS
  We adopted guinea-pig heart Langendorff instillation. The myocardial cells were isolated by collagenase (Type I, sigma)and marked by fluorescence ratio imaging. The suspension of myocardial cells was assigned to six groups: DHC, Ver, and control were each two. Each three groups was exposed to hypoxia and normoxia before determination of [Ca2+]i.
  
  
  RESULTS
  (1) In normoxia state, [Ca2+]i was 120.5-8.3 nml/L (n = 20).( 2) In hypoxia state, the increased [Ca2+]i of myocardial cells was proportional to the time (degree) of hypoxia. Correlation coefficient (r) was about 0.98. (3) Under the condition of normoxia DHC and Ver decreased [Ca2+]i. (4) DHC was obviously slow the increase of [Ca2+]i after hypoxia.
  
  
  CONCLUSIONS
  In normoxia and hypoxia, DHC decreases the increased [Ca2+]i. It can prevent intracellular calcium overload. We believe DHC may improve self-protected performance of myocardial cells.
authors.affiliation: Department of Physiology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Qin
authors.forename: Shan-Fen
authors.initials: SF
authors.lastname: Guo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D005260:Female; D006168:Guinea Pigs; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000470:Alkaloids; C007232:dehydrocorydalin; D002118:Calcium