title: A study on effects of glutathione s-transferase from silkworm on CCL4-induced mouse liver injury.
abstract: To assess the hepatoprotective activity of Glutathione S-transferase(GSTsw), extracted and purified from silkworm, in experimental acute mice liver injury and explore mechanisms. Mice were divided into five groups: control group, carbon tetrachloride (CCl4) group, and three treatment groups that received CCl4 and GSTsw at doses of 0.083 mg•g(-1), 0.0415 mg•g(-1) and 0.0207 mg•g(-1) for 3 days. ALT in serum, GST, SOD and T-AOC in liver tissue homogenate, and changes in liver pathology in the five groups were studied. CCl4 administration led to pathological and biochemical evidence of liver injury as compared to untreated controls. GSTsw administration led to significant protection against CCl4-induced changes in liver pathology. It was also associatedwith significantly lower serum ALT levels, higher GST-SOD and T-AOC level in live tissue homogenate. Thus, GSTsw showed protective activity against CCl4-induced hepatotoxicity in mice.
authors.affiliation: Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China. yanh1006@163.com
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Zhongzheng
authors.initials: Z
authors.lastname: Gui
authors.forename: Bochu
authors.initials: B
authors.lastname: Wang
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D012831:Bombyx / Q000201:enzymology*; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000473:pathology; D002463:Cell Membrane Permeability; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy*; D005609:Free Radicals / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism / Q000627:therapeutic use*; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008111:Liver Function Tests; D051379:Mice; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D007202:Indicators and Reagents; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000410:Alanine Transaminase