title: Improved bioprocess with CHO-hTSH cells on higher microcarrier concentration provides higher overall biomass and productivity for rhTSH.
abstract: Since the recombinant thyroid-stimulating hormone (rhTSH) is secreted by stably transfected Chinese hamster ovary (CHO-hTSH) cells, a bioprocess consisting of immobilizing the cells on a substrate allowing their multiplication is very suitable for rhTSH recovering from supernatants at relative high degree of purity. In addition, such a system has also the advantage of easily allowing delicate manipulations of culture medium replacement. In the present study, we show the development of a laboratory scale bioprocess protocol of CHO-hTSH cell cultures on cytodex microcarriers (MCs) in a 1 L bioreactor, for the preparation of rhTSH batches in view of structure/function studies. CHO-hTSH cells were cultivated on a fetal bovine serum supplemented medium during cell growth phase. For rhTSH synthesis phase, 75% of supernatant was replaced by animal protein-free medium every 24 h. Cell cultures were monitored for agitation (rpm), temperature (°C), dissolved oxygen (% DO), pH, cell concentration, MCs coverage, glucose consumption, lactate production, and rhTSH expression. The results indicate that the amount of MCs in the culture and the cell concentration at the beginning of rhTSH synthesis phase were crucial parameters for improving the final rhTSH production. By cultivating the CHO-hTSH cells with an initial cell seeding of four cells/MC on 4 g/L of MCs with a repeated fed batch mode of operation at 40 rpm, 37 °C, 20% DO, and pH 7.2 and starting the rhTSH synthesis phase with 3 × 10(6) cells/mL, we were able to supply the cultures with enough glucose, to maintain low levels of lactate, and to provide high percent (∼80%) of fully covered MCs for a long period (5 days) and attain a high cell concentration (∼9 × 10(5) cells/mL). The novelty of the present study is represented by the establishment of cell culture conditions allowing us to produce ∼1.6 mg/L of rhTSH in an already suitable degree of purity. Batches of produced rhTSH were purified and showed biological activity.
authors.affiliation: Laboratório de Imunologia Viral, Instituto Butantan, São Paulo, Brazil.
authors.forename: Daniella C
authors.initials: DC
authors.lastname: Ventini
authors.forename: Renata
authors.initials: R
authors.lastname: Damiani
authors.forename: Alvaro P B
authors.initials: AP
authors.lastname: Sousa
authors.forename: João E
authors.initials: JE
authors.lastname: de Oliveira
authors.forename: Cibele N
authors.initials: CN
authors.lastname: Peroni
authors.forename: Maria T C P
authors.initials: MT
authors.lastname: Ribela
authors.forename: Paolo
authors.initials: P
authors.lastname: Bartolini
authors.forename: Aldo
authors.initials: A
authors.lastname: Tonso
authors.forename: Carlos R J
authors.initials: CR
authors.lastname: Soares
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Pereira
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D013696:Temperature; D013972:Thyrotropin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D013972:Thyrotropin; D010100:Oxygen