title: Highly sensitive and selective derivatization-LC method for biomolecules based on fluorescence interactions and fluorous separations.
abstract: A fluorescence derivatization LC method is a powerful tool for the analysis with high sensitivity and selectivity of biological compounds. In this review, we introduce new types of fluorescence derivatization LC analysis methods. These are (1) detection-selective derivatization methods based on fluorescence interactions generated from fluorescently labeled analytes: excimer fluorescence derivatization and fluorescence resonance energy transfer (FRET) derivatization; (2) separation-selective derivatization methods using the fluorous separation technique: fluorous derivatization, F-trap fluorescence derivatization, and fluorous scavenging derivatization (FSD).
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Fukuoka 814-0180, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Todoroki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hayama
authors.forename: Miki
authors.initials: M
authors.lastname: Itoyama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nohta
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D010636:Phenols / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D059808:Polyphenols; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002264:Carboxylic Acids; D005419:Flavonoids; D005456:Fluorescent Dyes; D010636:Phenols; D011073:Polyamines; D059808:Polyphenols; D013438:Sulfhydryl Compounds