title: Expression of genes of Rhizoctonia solani and the biocontrol Stachybotrys elegans during mycoparasitism of hyphae and sclerotia.
abstract: Knowledge of mycoparasitism has been focused on how antagonists affect pathogens in relation to mechanisms, metabolites and gene expression. Just as microbial antagonists use a diverse arsenal of mechanisms to dominate interactions with hosts, hosts also have diverse responses to counteract antagonism. In this study differential gene expression of eight mycoparasitism-induced genes and eight host-response genes was monitored during in vivo interactions between the mycoparasite Stachybotrys elegans and hyphae and sclerotia of the host, Rhizoctonia solani over 5 d of interaction. Using real time reverse transcription polymerase chain reaction, comparative analyses demonstrated that hyphal and sclerotial structures triggered different expression patterns. These results indicated that multiple regulatory mechanisms might be involved. The high elevated expression of some genes belonging to the mycoparasite and the host suggest that these genes play an important role during the mycoparasitic process and host defense respectively.
authors.affiliation: Department of Plant Science, Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Quebec H9X 3V9.
authors.forename: Rony
authors.initials: R
authors.lastname: Chamoun
authors.forename: Suha
authors.initials: S
authors.lastname: Jabaji
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000898:Antibiosis; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000502:physiology*; D056265:Microbial Interactions; D008853:Microscopy; D025282:Mycelium / Q000502:physiology*; D012331:RNA, Fungal / Q000032:analysis / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012232:Rhizoctonia / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012988:Soil Microbiology; D013191:Stachybotrys / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D017382:Reactive Oxygen Species; D000429:Alcohol Oxidoreductases; C022495:pyridoxine 4-dehydrogenase