title: The role of mannose-binding lectin gene polymorphisms in susceptibility to HIV-1 infection in Southern Brazilian patients.
abstract: OBJECTIVE
  This study investigates the role of mannose-binding lectin (MBL) in the susceptibility to HIV-1 infection analyzing polymorphisms located at the MBL2 promoter and exon 1 regions.
  
  
  METHODS
  The prevalence of MBL2 variant alleles was investigated in 410 HIV-1-infected patients from the South Brazilian HIV cohort and in 345 unexposed uninfected healthy individuals. The promoter variants were genotyped using polymerase chain reaction with sequence-specific primers (PCR-SSP) and exon 1 variants were analyzed by real-time PCR using a melting temperature assay and were confirmed by PCR-restriction fragment length polymorphism (RFLP). MBL2 genotypic and allelic frequencies were compared between HIV-1-infected patients and controls using the chi-squared tests.
  
  
  RESULTS
  The analyses were performed subdividing the individuals according to their ethnic origin. Among Euro-derived individuals a higher frequency of the LX/LX genotype was observed in patients when compared to controls (P < 0.001). The haplotypic analysis also showed a higher frequency of the haplotypes associated with lower MBL levels among HIV-1-infected patients (P = 0.0001). Among Afro-derived individuals the frequencies of LY/LY and HY/HY genotypes were higher in patients when compared to controls (P = 0.009 and P = 0.02).
  
  
  CONCLUSIONS
  An increased frequency of MBL2 genotypes associated with low MBL levels was observed in Euro-derived patients, suggesting a potential role for MBL in the susceptibility to HIV-1 infection in Euro-derived individuals.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Gabriela Kniphoff
authors.initials: GK
authors.lastname: da Silva
authors.forename: Rafael
authors.initials: R
authors.lastname: Guimarães
authors.forename: Vanessa Suñé
authors.initials: VS
authors.lastname: Mattevi
authors.forename: Rosmeri Kuhmmer
authors.initials: RK
authors.lastname: Lazzaretti
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sprinz
authors.forename: Regina
authors.initials: R
authors.lastname: Kuhmmer
authors.forename: Lucas
authors.initials: L
authors.lastname: Brandão
authors.forename: Sérgio
authors.initials: S
authors.lastname: Crovella
authors.forename: José Artur Bogo
authors.initials: JA
authors.lastname: Chies
mesh_terms: D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D015497:HIV-1; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology; D008297:Male; D000090323:Mannose Receptor; D037601:Mannose-Binding Lectin / Q000235:genetics* / Q000276:immunology; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000276:immunology; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: D037181:Lectins, C-Type; C445619:MBL2 protein, human; D000090323:Mannose Receptor; D037601:Mannose-Binding Lectin; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface