title: Radical scavenging and antioxidant activities of isocoumarins and a phthalide from the endophytic fungus Colletotrichum sp.
abstract: Five known isocoumarins, monocerin (1), derivative 2, and fusarentin derivatives 3-5, and a new phthalide (6) were isolated from the endophytic fungus Colletotrichum sp. 2 selectively exhibited cytotoxic activity toward the HepG2 cell line. Compounds 2 and 4 scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals (IC(50) values of 23.4 and 16.4 μM, respectively) and inhibited superoxide anion radical formation (IC(50) values of 52.6 and 4.3 μM, respectively). The C-7 hydroxyl group in 2 and 4 might be important for radical scavenging activities. Isocoumarins 1-3 and phthalide 6 showed potent antioxidant activity.
authors.affiliation: Chulabhorn Graduate Institute, Vibhavadi-Rangsit Road, Laksi, Bangkok 10210, Thailand.
authors.forename: Kamolchanok
authors.initials: K
authors.lastname: Tianpanich
authors.forename: Surasak
authors.initials: S
authors.lastname: Prachya
authors.forename: Suthep
authors.initials: S
authors.lastname: Wiyakrutta
authors.forename: Chulabhorn
authors.initials: C
authors.lastname: Mahidol
authors.forename: Somsak
authors.initials: S
authors.lastname: Ruchirawat
authors.forename: Prasat
authors.initials: P
authors.lastname: Kittakoop
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047072:Aromatase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D020231:Colletotrichum / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D018922:HL-60 Cells; D056945:Hep G2 Cells; D006801:Humans; D049934:Isocoumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D010851:Picrates / Q000494:pharmacology; D031701:Piper / Q000382:microbiology; D013329:Structure-Activity Relationship; D013785:Thailand
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D047072:Aromatase Inhibitors; D001572:Benzofurans; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D049934:Isocoumarins; D007783:Lactones; D010851:Picrates; C082022:phthalide; C004931:1,1-diphenyl-2-picrylhydrazyl; C485193:monocerin