title: [The effects of urea on ECG and the sodium currents of ventricular myocyte in mice].
abstract: OBJECTIVE
  To observe the effects of urea on ECG and sodium currents of ventricular myocyte in mice.
  
  
  METHODS
  ECG and patch clamp techniques were used in the experiments, to record ECG of mice and sodium currents of ventricular myocyte in mice.
  
  
  RESULTS
  Urea could lead mice heart rate evidently slow down (P < 0.01) with concentration dependent. The heart rate were (556 +/- 29, 469 +/- 37, 378 +/- 48) b minT in low, middle, high groups respectively before using urea and (612 +/- 27, 615 +/- 23, 619 +/- 26) x min(-1) after. The conduction block arrhythmia was happened in middle and high groups. The sodium currents of ventricular myocyte in mice was inhibited by urea(P < 0.05). The sodium currents amplitude value were reduced to (7.32 +/- 0.68, 5.69 +/- 0.64, 4.58 +/- 0.57) nA after using urea in each group and were (8.76 +/- 0.91, 8.87 +/- 1.01, 8.77 +/- 0.96) nA before, submit concentration dependent.
  
  
  CONCLUSIONS
  Urea can inhibit the sodium currents of ventricular myocyte in mice to make it happen conduction block arrhythmia.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Xue-xin
authors.initials: XX
authors.lastname: Zhang
authors.forename: Shu-gang
authors.initials: SG
authors.lastname: Xi
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D004562:Electrocardiography; D005260:Female; D006352:Heart Ventricles / Q000166:cytology; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D015222:Sodium Channels / Q000378:metabolism / Q000502:physiology*; D014508:Urea / Q000378:metabolism*
chemical_list: D026901:Membrane Transport Proteins; D015222:Sodium Channels; C452857:urea transporter B, mouse; D014508:Urea