title: Characterization of the pituitary tumor transforming gene 1 knockout mouse retina.
abstract: Recent gene expression studies on mouse models for retinal degeneration identified deregulation of Pituitary tumor transforming gene 1 (Pttg1) as a potential susceptibility factor involved in photoreceptor cell death. Pttg1 is a transcription regulatory protein involved in sister chromatid segregation, and Pttg1(-/-) mice exhibit testicular and splenic hypoplasia, thymic hyperplasia, aberrant cell cycle progression, chromosome instability, and impaired glucose homeostasis leading to diabetes, particularly in older males. Due to Pttg1 deregulation in dystrophic retinas, we characterized Pttg1(-/-) retinas using Hematoxylin and Eosin (H&E) staining, immunohistochemistry (IHC), and electroretinography (ERG). Seven month old Pttg1(-/-) mice were also examined for a diabetic retinopathy phenotype using Fluorescein Angiography (FA) to test for neovascularization. Our data reveal that up to 9 months of age, Pttg1(-/-) retinas have a healthy morphology and normal photoreceptor function. This study lays the groundwork for further investigation into the relevance of Pttg1 in retinal dystrophy.
authors.affiliation: Division of Retinal Molecular Biology, Department of Ophthalmology, The Mary D. Allen Laboratory for Vision Research, Doheny Eye Institute, Los Angeles, CA 90033, USA.
authors.forename: Rosanne M
authors.initials: RM
authors.lastname: Yetemian
authors.forename: Cheryl M
authors.initials: CM
authors.lastname: Craft
mesh_terms: D000818:Animals; D001483:Base Sequence; D020256:Choroidal Neovascularization / Q000235:genetics; D017931:DNA Primers; D004596:Electroretinography; D005260:Female; D005451:Fluorescein Angiography; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009363:Neoplasm Proteins / Q000235:genetics*; D012160:Retina / Q000378:metabolism*; D064249:Securin
chemical_list: D017931:DNA Primers; D009363:Neoplasm Proteins; D064249:Securin