title: [Establishment of heterologous expression model of hSERT in Xenopus laevis oocytes].
abstract: OBJECTIVE
  To determine the feasibility of establishing the heterologous expression model of human- serotonin transporter(hSERT or 5-HTT).
  
  
  METHODS
  cRNA of SERT was transcribed from cDNA, which was cloned in the pOTV vector. Each oocyte of mature xenopus laevis was injected with transcribed cRNA in vivo and incubated at room temperature for 4-9 days. Recording the current induced by 5-HT with voltage clamp technique tested the function of the expressed 5-HT transporter.
  
  
  RESULTS
  The transporter current could be observed in Ringer's solution containing 5-HT, and the 5-HT induced current were concentration-dependent. Norepinephrine and dopamine could not induce the transporter current while the 5-HT induced current could be specifically inhibited by 5-HTT blocker, desipramine.
  
  
  CONCLUSIONS
  The results demonstrate that the heterologous expression product in xenopus laevis oocytes is human 5-HT transporter.
authors.affiliation: Department of Physiology, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: Yi-Ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Jin
authors.forename: Ci-Zhen
authors.initials: CZ
authors.lastname: Li
authors.forename: Yuan-Mou
authors.initials: YM
authors.lastname: Liu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D023421:Models, Animal; D009865:Oocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012701:Serotonin / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin