title: Parathyroid hormone-related peptide directly increases adrenocorticotropic hormone secretion from the anterior pituitary in hens.
abstract: The presence of the receptor for parathyroid hormone-related peptide (PTHrP) and the effect of PTHrP on adrenocorticotropic hormone (ACTH) secretion in the hen anterior pituitary were examined. The plasma membrane fraction of the anterior pituitary was found to contain a specific chicken PTHrP (cPTHrP) binding component. The binding component had properties of a receptor, such as binding specificity to cPTHrP, reversibility, saturable binding, high affinity, and limited capacity; therefore, it was elucidated that the PTHrP receptor exists in the plasma membrane of the hen anterior pituitary. A third ventricular injection of cPTHrP in nonlaying hens caused a decrease in the chicken ACTH level of the anterior pituitary and an increase in the chicken ACTH level of blood plasma, with an increase in the binding affinity and a decrease in the binding capacity of PTHrP receptor in the anterior pituitary. The present study suggests that PTHrP may act directly on the anterior pituitary via its receptor binding and may enhance ACTH secretion from this tissue in hens.
authors.affiliation: The United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Oomatsu
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa-Mizuyachi
authors.forename: M
authors.initials: M
authors.lastname: Kawashima
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D005260:Female; D044162:Parathyroid Hormone-Related Protein / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D044162:Parathyroid Hormone-Related Protein; D000324:Adrenocorticotropic Hormone