title: The effect of toremifene on lipid metabolism compared with that of tamoxifen in vitro.
abstract: BACKGROUND
  Tamoxifen (TAM) and toremifene (TOR) prevent estrogen from stimulating breast cancer growth and also have agonistic effects in a number of physiological systems. TAM is known to increase intracellular triglyceride, but the action of TOR on lipid metabolism in vitro has not yet been determined.
  
  
  OBJECTIVE
  To compare the effect of TOR on lipid metabolism with that of TAM in vitro, using HepG2 cell lines.
  
  
  METHODS
  Intracellular concentrations of total cholesterol and triglyceride in HepG2 cells were measured by an enzymatic method after TAM or TOR treatment.
  
  
  RESULTS
  Intracellular concentrations of total cholesterol were decreased by both TAM and TOR, but not significantly different from the control level. TOR produced no changes in the intracellular concentrations of triglyceride, whereas TAM increased the intracellular concentrations of triglyceride at concentrations ranging from 10(-7) to 10(-5) mol/l of TAM (p < 0.05). Moreover, significant differences were noted between the two treated groups at concentrations ranging from 10(-9) to 10(-5) mol/l (p < 0.05).
  
  
  CONCLUSIONS
  TOR treatment did not increase intracellular concentrations of triglyceride, although TAM treatment did so.
authors.affiliation: Department of Breast and Endocrine Surgery, Nagoya University School of Medicine, Nagoya, Japan. m-sawaki@med.nagoya-u.ac.jp
authors.forename: Masataka
authors.initials: M
authors.lastname: Sawaki
authors.forename: Ai
authors.initials: A
authors.lastname: Idota
authors.forename: Hiroki
authors.initials: H
authors.lastname: Uchida
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Noda
authors.forename: Shigenori
authors.initials: S
authors.lastname: Sato
authors.forename: Toyone
authors.initials: T
authors.lastname: Kikumori
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Imai
mesh_terms: D002784:Cholesterol / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology*; D017312:Toremifene / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D020845:Selective Estrogen Receptor Modulators; D014280:Triglycerides; D013629:Tamoxifen; D017312:Toremifene; D002784:Cholesterol