title: [Construction of a lentivirus interfering vector targeting Cyc1 and its interfering efficiency in nasopharyngeal carcinoma cells].
abstract: OBJECTIVE
  To analyze the expression of Cyc1 in nasopharyngeal carcinoma (NPC) and evaluate the interfering efficiency of a lentivirus interfering vector targeting Cyc1 in NPC cells.
  
  
  METHODS
  Microarray technique was used to examine the expression of Cyc1 in NPC tissues. Real-time PCR was utilized to confirm the high expression of Cyc1 in NPC tissues and NPC cell lines. The recombinant Cyc1 shRNA-expressing plasmid (pLentiU6/Cyc1-shRNA) was stably transfected into NPC cells, and the interfering efficiency against Cyc1 was evaluated by quantitative RT-PCR.
  
  
  RESULTS
  The result of microarray showed that Cyc1 was highly expressed in NPC tissues compared to noncancerous nasopharyngeal tissues, as confirmed by Real-time PCR. All of the 8 NPC cells showed a high expression of Cyc1, among which 5-8F cells showed the highest expression. Sequence analysis indicated that the recombinant plasmid pLentiU6/Cyc1-shRNA was successfully constructed and could significantly and stably suppress the expression of Cyc1 in NPC cells.
  
  
  CONCLUSIONS
  Cyc1 is highly expressed in NPC cells. The lentivirus vector constructed can markedly inhibit the expression of Cyc1 in NPC cells, which provides assistance in the investigation of the function and molecular mechanism of Cyc1 in NPC.
authors.affiliation: Department of Pathology, Guangzhou Medical College, China. narcissus_jane@163.com
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Long
authors.forename: Wei-yi
authors.initials: WY
authors.lastname: Fang
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D003575:Cytochromes c1 / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D003575:Cytochromes c1