title: Chromosome painting for plant biotechnology.
abstract: Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress. This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on somatic metaphase and meiotic pachytene spreads.
authors.affiliation: Division of Biological Sciences, University of Missouri, Columbia, MO, USA.
authors.forename: Akio
authors.initials: A
authors.lastname: Kato
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Lamb
authors.forename: Patrice S
authors.initials: PS
authors.lastname: Albert
authors.forename: Tatiana
authors.initials: T
authors.lastname: Danilova
authors.forename: Fangpu
authors.initials: F
authors.lastname: Han
authors.forename: Zhi
authors.initials: Z
authors.lastname: Gao
authors.forename: Seth
authors.initials: S
authors.lastname: Findley
authors.forename: James A
authors.initials: JA
authors.lastname: Birchler
mesh_terms: D001709:Biotechnology / Q000379:methods; D020223:Chromosome Painting / Q000379:methods*; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008677:Metaphase; D010944:Plants / Q000235:genetics*
chemical_list: D018744:DNA, Plant