title: Impact of anaerobic and oligotrophic conditions on survival of Alloiococcus otitidis, implicated as a cause of otitis media.
abstract: The survival of Alloiococcus otitidis (NCFB2890) with different nutritional supplements, including brain-heart infusion broth (BHI), phosphate-buffered saline (PBS), distilled water (DW), and middle ear effusion (MEE), as well as various atmospheres (aerobic, microaerobic, anaerobic), was compared using cultures, LIVE/DEAD staining, and transmission electron microscopy. The bacterial morphological traits and viability were maintained in BHI and MEE under aerobic conditions but were rapidly lost in PBS and DW. In contrast, anaerobic conditions did not support viability at all. Thus, the bacteria critically required an aerobic atmosphere for its survival as well as the appropriate nutrients, implying that culture of this pathogen from clinical specimens would become more difficult through oxygen depletion depending on a slight change in the middle ear atmosphere.
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Health Sciences, Hokkaido University, Nishi-5 Kita-12, Kitaku, Sapporo, Hokkaido, 060-0812, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Matsuo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Harimaya
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukumoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kaori
authors.initials: K
authors.lastname: Takahashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iida
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Fujii
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamaguchi
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D056568:Carnobacteriaceae / Q000378:metabolism / Q000502:physiology*; D002648:Child; D015169:Colony Count, Microbial; D003470:Culture Media; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010033:Otitis Media / Q000382:microbiology*; D010100:Oxygen / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010100:Oxygen