title: A tissue-specific atlas of mouse protein phosphorylation and expression.
abstract: Although most tissues in an organism are genetically identical, the biochemistry of each is optimized to fulfill its unique physiological roles, with important consequences for human health and disease. Each tissue's unique physiology requires tightly regulated gene and protein expression coordinated by specialized, phosphorylation-dependent intracellular signaling. To better understand the role of phosphorylation in maintenance of physiological differences among tissues, we performed proteomic and phosphoproteomic characterizations of nine mouse tissues. We identified 12,039 proteins, including 6296 phosphoproteins harboring nearly 36,000 phosphorylation sites. Comparing protein abundances and phosphorylation levels revealed specialized, interconnected phosphorylation networks within each tissue while suggesting that many proteins are regulated by phosphorylation independently of their expression. Our data suggest that the "typical" phosphoprotein is widely expressed yet displays variable, often tissue-specific phosphorylation that tunes protein activity to the specific needs of each tissue. We offer this dataset as an online resource for the biological research community.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Edward L
authors.initials: EL
authors.lastname: Huttlin
authors.forename: Mark P
authors.initials: MP
authors.lastname: Jedrychowski
authors.forename: Joshua E
authors.initials: JE
authors.lastname: Elias
authors.forename: Tapasree
authors.initials: T
authors.lastname: Goswami
authors.forename: Ramin
authors.initials: R
authors.lastname: Rad
authors.forename: Sean A
authors.initials: SA
authors.lastname: Beausoleil
authors.forename: Judit
authors.initials: J
authors.lastname: Villén
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Haas
authors.forename: Mathew E
authors.initials: ME
authors.lastname: Sowa
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D051379:Mice / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics
chemical_list: D011506:Proteins; D011494:Protein Kinases