title: Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice.
abstract: We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P<0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat.
authors.affiliation: Teagasc Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland.|Department of Microbiology, University College Cork, National University of Ireland, Co. Cork, Ireland.|Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Eva
authors.initials: E
authors.lastname: Rosberg-Cody
authors.affiliation: Teagasc Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland.|Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
authors.affiliation: Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Liam
authors.initials: L
authors.lastname: O'Mahony
authors.affiliation: Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wall
authors.affiliation: Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Fergus
authors.initials: F
authors.lastname: Shanahan
authors.affiliation: Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Eamonn M
authors.initials: EM
authors.lastname: Quigley
authors.affiliation: Department of Microbiology, University College Cork, National University of Ireland, Co. Cork, Ireland.|Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.affiliation: Teagasc Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland.|Alimentary Pharmabiotic Centre (APC), Cork, Ireland.
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D005772:Gastrointestinal Transit; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism; D019787:Linoleic Acid / Q000008:administration & dosage*; D044243:Linoleic Acids, Conjugated / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D011425:Propionibacterium acnes / Q000201:enzymology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C094849:10,12-octadecadienoic acid; D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D011994:Recombinant Proteins; D019787:Linoleic Acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase