title: Highly sensitive and rapid detection of microcystin-LR in source and finished water samples using cantilever sensors.
abstract: Microcystin-leucine-arginine (MCLR) is one of the toxic microcystin congeners produced by the common cyanobacteria, blue-green algae. A piezoelectric-excited millimeter-sized cantilever (PEMC) sensor was developed for the sensitive detection of MCLR in a flow format using both monoclonal and polyclonal antibodies that bind specifically to MCLR. PEMC is a resonant cantilever sensor whose resonant frequency decreases as target analyte binds to its surface. Monoclonal antibody against MCLR was immobilized on the sensor surface via amine coupling. As the toxin in the sample water bound to the antibody, resonant frequency decreased proportional to toxin concentration. Three water matrices, namely buffer, tap water, and river water, were spiked with MCLR standards and were successfully detected in the dynamic range of 1 pg/mL to 100 ng/mL (effective concentration -250 fg/mL to 25 ng/mL). The sensor response was characterized by a log-linear relationship between resonant frequency change and MCLR concentration. Positive verification of MCLR detection was confirmed by a sandwich binding on the sensor with a second antibody binding to MCLR on the sensor (attached in first detection step) which caused a further resonant frequency decrease. We show for the first time that MCLR in various water samples can be detected at 1 pg/mL.
authors.affiliation: Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Ding
authors.forename: Raj
authors.initials: R
authors.lastname: Mutharasan
mesh_terms: D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques / Q000379:methods*; D000458:Cyanobacteria; D005618:Fresh Water; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; D014873:Water Pollutants; C057862:cyanoginosin LR