title: Preparation of a silk fibroin spongy wound dressing and its therapeutic efficiency in skin defects.
abstract: A novel silk fibroin spongy wound dressing (SFSD) incorporated with nano-Ag particles was prepared by coagulating with 1.25-5.0% (v/v) poly(ethylene glycol diglycidyl ether) (PGDE). The mechanical properties, moisture permeability and hygroscopicity of SFSD, and the nano-Ag release  behavior from SFSD were evaluated. The results showed that the soft SFSD had satisfying tensile strength and flexibility, as well as excellent moisture permeability and absorption capability of wound exudates. The moisture permeability was 101 g/m(2) per h and the water absorption  capacity of SFSD was 595.2% and 251.9% of its own weight in dry and wet states, respectively. The nano-Ag in the SFSD was released continuously at a relatively stable rate in PBS resulting in a remarkable antibacterial property. A rabbit model was used to dynamically observe the healing process  of full-thickness skin defects. Full-thickness wounds were created on the dorsal side of rabbits, which were covered with SFSD and porcine acellular dermal matrix (PADM) for comparison. The mean healing time of the wounds covered with SFSD was 17.7 ± 2.4 days, significantly shorter  than that with PADM. The histological analysis showed that the epidermal cell layer formed with SFSD was very similar to normal skin, suggesting that SFSD may provide a good component for the development of new wound dressings.
authors.affiliation: Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, P. R. China. minsj@zju.edu.cn
authors.forename: Sijia
authors.initials: S
authors.lastname: Min
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.forename: Chunmao
authors.initials: C
authors.lastname: Han
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Mingying
authors.initials: M
authors.lastname: Yang
authors.forename: Liangjun
authors.initials: L
authors.lastname: Zhu
authors.forename: Haiping
authors.initials: H
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Juming
authors.initials: J
authors.lastname: Yao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001458:Bandages / Q000382:microbiology; D062109:Dermatologic Surgical Procedures; D004853:Epoxy Resins / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D055595:Mechanical Phenomena; D053768:Metal Nanoparticles / Q000737:chemistry; D010539:Permeability; D011817:Rabbits; D012834:Silver / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D015503:Surgical Sponges / Q000382:microbiology; D013552:Swine; D017155:Wettability; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004853:Epoxy Resins; C035364:Quetol 651; D012834:Silver; D005349:Fibroins