title: Rectal swab sampling followed by an enrichment culture-based real-time PCR assay to detect Salmonella enterocolitis in children.
abstract: Although routine bacterial culture is the traditional reference standard method for the detection of Salmonella infection in children with diarrhoea, it is a time-consuming procedure that usually only gives results after 3-4 days. Some molecular detection methods can improve the turn-around time to within 24 h, but these methods are not applied directly from stool or rectal swab specimens as routine diagnostic methods for the detection of gastrointestinal pathogens. In this study, we tested the feasibility of a bacterial enrichment culture-based real-time PCR assay method for detecting and screening for diarrhoea in children caused by Salmonella. Our results showed that the minimum real-time PCR assay time required to detect enriched bacterial culture from a swab was 3 h. In all children with suspected Salmonella diarrhoea, the enrichment culture-based real-time PCR achieved 85.4% sensitivity and 98.1% specificity, as compared with the 53.7% sensitivity and 100% specificity of detection with the routine bacterial culture method. We suggest that rectal swab sampling followed by enrichment culture-based real-time PCR is suitable as a rapid method for detecting and screening for Salmonella in paediatric patients.
authors.affiliation: Department of Paediatrics, Cathay General Hospital, Sijhih, Taipei, Taiwan, Republic of China.
authors.forename: L-H
authors.initials: LH
authors.lastname: Lin
authors.forename: C-Y
authors.initials: CY
authors.lastname: Tsai
authors.forename: M-H
authors.initials: MH
authors.lastname: Hung
authors.forename: Y-T
authors.initials: YT
authors.lastname: Fang
authors.forename: Q-D
authors.initials: QD
authors.lastname: Ling
mesh_terms: D018929:Cell Culture Techniques; D002648:Child; D015331:Cohort Studies; D003967:Diarrhea / Q000382:microbiology; D004760:Enterocolitis / Q000382:microbiology*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012007:Rectum / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification*; D012478:Salmonella Food Poisoning / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity