title: [Role of TNF-alpha in vascular endothelial cells injury mediated by frozen/thawed PMN].
abstract: OBJECTIVE
  To investigate the role of TNF-alpha in vascular endothelial cells injury mediated by freezing/thaw ing PMN.
  
  
  METHODS
  Freezing/thawing cell model was founded using rat PMN isolated by dextran sedimentation technique and VEC cultured in vitro. The injury level of VEC was indicated by measuring activity of LDH in medium. The number of frozen/thawed PMN adhering to VEC was counted with Phagocytizing reactive dyes the degree of frozen/thawed PMN and VEC adhesion. Expression of LFA-1 on the surface of frozen/thawed PMN was analyzed with flow cytometry.
  
  
  RESULTS
  TNF-alpha could obviously upregulate expression of LFA-1 on surfaced of frozen/thawed PMN. Upregulation of LFA-1 expression promoted adhesion of frozen/thawed PMN and normal VEC,and aggravated VEC injury. Monoclonal antibody against LFA-1 could partly block adhesion of frozen/thawed PMN and normal VEC,and attenuate VEC injury.
  
  
  CONCLUSIONS
  TNF-alpha can promote expression of LFA-1 on surface of frozen/thawed PMN adhering of frozen/thawed PMN to normal VEC and VEC injury increase, monoclonal antibody against LFA-1 could partly block PMN-VEC adhesion and attenuate VEC injury.
authors.affiliation: Institute of Health and Environment Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Jin
authors.forename: Jia-Jing
authors.initials: JJ
authors.lastname: Liu
authors.forename: Zeng-Ren
authors.initials: ZR
authors.lastname: Yang
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Yan
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology; D005615:Freezing; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D014409:Tumor Necrosis Factor-alpha