title: Oxidative degradation of Remazol Turquoise Blue G 133 by soybean peroxidase.
abstract: Reactive dyes are widely employed in textile industries and their removal from wastewaters is a relevant environmental problem. In addition to chemical and physical methods, several bioremediation approaches, involving intact micro-organisms or isolated enzymes, have been proposed to decolorize dye solutions. In this paper, we report the complete and fast decolourization of a Cu(II)-phthalocyanine based reactive dye (Remazol Turquoise Blue G 133) by means of the soybean peroxidase/H(2)O(2) system. The oxidative degradation of the dye in aqueous solution at 25°C was studied as function of pH, revealing a quantitative decolourization yield at acidic pH values with a maximum of activity at pH 3.3. The reaction products were identified and characterized by HPLC-diode array detector (DAD)-mass spectrometry (MS), ionic chromatography and EPR techniques. This analysis showed that the enzyme catalyses the breaking of the phthalocyanine ring producing sulfophthalimide as the main degradation product, and the release of stoichiometric amount of ammonium and Cu(II) ions.
authors.affiliation: Università degli Studi di Torino, Department of Chemistry I.F.M., Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Marchis
authors.forename: Paola
authors.initials: P
authors.lastname: Avetta
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bianco-Prevot
authors.forename: Debora
authors.initials: D
authors.lastname: Fabbri
authors.forename: Guido
authors.initials: G
authors.lastname: Viscardi
authors.forename: Enzo
authors.initials: E
authors.lastname: Laurenti
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*; D013025:Glycine max / Q000201:enzymology*
chemical_list: D056831:Coordination Complexes; D007211:Indoles; C558160:remazol turquoise blue G 133; D006861:Hydrogen Peroxide; D009195:Peroxidase