title: [Effect of valproic acid on apoptosis of leukemia HL-60 cells and expression of h-tert gene].
abstract: This study was aimed to clarify whether valproic acid (VPA) induces apoptosis of leukemia HL-60 cell line and its possible mechanism. The effect of different concentrations and treatment time of VPA on HL-60 cell proliferation was assayed by cytotoxicity test (CCK-8 method) and fluorescence microscopy, and flow cytometry was used to detect cell apoptosis. The expressions of telomerase subunit h-tert mRNA and apoptosis-related protein as well as caspase-3 activity were detected by real time-quantitative PCR, Western blot and ELISA respectively. The results indicated that VPA inhibited proliferation of HL-60 cells and induced cell apoptosis in a dose dependent manner (r = -0.87). The expressions of anti-apoptotic protein BCL-2 and h-tert mRNA were significantly decreased while the pro-apoptotic protein BAX and caspase-3 activity increased after treatment with VPA. The apoptosis rate of HL-60 cell was negatively correlated with expression of h-tert mRNA. It is concluded that VPA can inhibit leukemia HL-60 cell proliferation and induce apoptosis. The VPA displays anti-leukemia activity possibly through reducing h-tert mRNA and BCL-2 protein expression, increasing BAX expression and activity of caspase-3.
authors.affiliation: Department of Hematology, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Yi-Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Song-Mei
authors.initials: SM
authors.lastname: Yin
authors.forename: Si-Qiong
authors.initials: SQ
authors.lastname: Feng
authors.forename: Da-Nian
authors.initials: DN
authors.lastname: Nie
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Xie
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ma
authors.forename: Xiu-Ju
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yu-Dan
authors.initials: YD
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism*; D014635:Valproic Acid / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D014635:Valproic Acid; C509186:TERT protein, human; D019098:Telomerase; C505407:CASP3 protein, human; D053148:Caspase 3