title: Effects of human growth hormone on gonadotropin-releasing hormone neurons in mice.
abstract: OBJECTIVE
  Recombinant human growth hormone (rhGH) has been widely used to treat short stature. However, there are some concerns that growth hormone treatment may induce skeletal maturation and early onset of puberty. In this study, we investigated whether rhGH can directly affect the neuronal activities of of gonadotropin-releasing hormone (GnRH).
  
  
  METHODS
  We performed brain slice gramicidin-perforated current clamp recording to examine the direct membrane effects of rhGH on GnRH neurons, and a whole-cell voltage-clamp recording to examine the effects of rhGH on spontaneous postsynaptic events and holding currents in immature (postnatal days 13-21) and adult (postnatal days 42-73) mice.
  
  
  RESULTS
  In immature mice, all 5 GnRH neurons recorded in gramicidin-perforated current clamp mode showed no membrane potential changes on application of rhGH (0.4, 1 µg/mL). In adult GnRH neurons, 7 (78%) of 9 neurons tested showed no response to rhGH (0.2-1 µg/mL) and 2 neurons showed slight depolarization. In 9 (90%) of 10 immature neurons tested, rhGH did not induce any membrane holding current changes or spontaneous postsynaptic currents (sPSCs). There was no change in sPSCs and holding current in 4 of 5 adult GnRH neurons.
  
  
  CONCLUSIONS
  These findings demonstrate that rhGH does not directly affect the GnRH neuronal activities in our experimental model.
authors.affiliation: Department of Oral Physiology & Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Janardhan P
authors.initials: JP
authors.lastname: Bhattarai
authors.forename: Shin Hye
authors.initials: SH
authors.lastname: Kim
authors.forename: Seong Kyu
authors.initials: SK
authors.lastname: Han
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Park