title: Volatile profiles of toxigenic and non-toxigenic Aspergillus flavus using SPME for solid phase extraction.
abstract: Toxigenic and atoxigenic strains of Aspergillus flavus were grown on potato dextrose agar (PDA) and wetted (23% moisture) sterile, cracked corn for 14 and 21 days, respectively. Volatile compounds produced by A. flavus, as well as those present in the PDA controls and sterile cracked maize, were collected using solid-phase micro-extraction (SPME) and identified by gas chromatography/mass spectrometry. Results show that growth substrate had a major impact on the number and type of volatiles detected. Growth on sterile cracked maize produced many more volatiles than did potato dextrose agar. There were also differences observed in the type of volatiles produced between toxigenic and non-toxigenic isolates, as well as between isolates of the same toxigenic grouping.
authors.affiliation: Southern Regional Research Center, USDA, ARS, New Orleans, LA 70124, USA. Anthony.DeLucca@ars.usda.gov
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: De Lucca
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Boué
authors.forename: Carol H
authors.initials: CH
authors.lastname: Carter-Wientjes
authors.forename: John M
authors.initials: JM
authors.lastname: Bland
authors.forename: Deepak
authors.initials: D
authors.lastname: Bhatnagar
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Cleveland
mesh_terms: D001231:Aspergillus flavus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D052617:Solid Phase Microextraction / Q000379:methods; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D055549:Volatile Organic Compounds