title: Honeybee (Apis mellifera ligustica) drone embryo proteomes.
abstract: Little attention has been paid to the drone honeybee (Apis mellifera ligustica) which is a haploid individual carrying only the set of alleles that it inherits from its mother. Molecular mechanisms underlying drone embryogenesis are poorly understood. This study evaluated protein expression profiles of drone embryogenesis at embryonic ages of 24, 48 and 72h. More than 100 reproducible proteins were analyzed by mass spectrometry on 2D electrophoresis gels. Sixty-two proteins were significantly changed at the selected three experimental age points. Expression of the metabolic energy requirement-related protein peaked at the embryonic age of 48h, whereas development and metabolizing amino acid-related proteins expressed optimally at 72h. Cytoskeleton, protein folding and antioxidant-related proteins were highly expressed at 48 and 72h. Protein networks of the identified proteins were constructed and protein expressions were validated at the transcription level. This first proteomic study of drone embryogenesis in the honeybee may provide geneticists an exact timetable and candidate protein outline for further manipulations of drone stem cells.
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Science/Key Laboratory of Pollinating Insect Biology, 1# Beigou, Xiangshan, Haidian District, Beijing, China. apislijk@126.com
authors.forename: Jianke
authors.initials: J
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Fang
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Desalegn
authors.initials: D
authors.lastname: Begna
mesh_terms: D000818:Animals; D001516:Bees / Q000737:chemistry* / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D058507:Host Specificity; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D020543:Proteome