title: Polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene and risk of head and neck squamous cell carcinoma.
abstract: Methylenetetrahydrofolate reductase gene (MTHFR) C677T polymorphism may be a risk factor for head and neck squamous cell carcinoma due to changes in folate levels that can induce disorders in the methylation pathway, which results in carcinogenesis.
  
  
  OBJECTIVE
  To evaluate MTHFR C677T polymorphism in patients with head and neck squamous cell carcinoma and in individuals with no history of cancer, and to assess the association of this disease with clinical histopathological parameters. SERIES AND METHODS: A retrospective study that assessed gender, age, tobacco, alcohol consumption and clinical histopathological parameters in 200 patients (100 with disease and 100 with no history of cancer). PCR-RFLP molecular analysis was carried out and the chi-square test and multiple logistic regression were applied for the statistical analysis.
  
  
  RESULTS
  There was no association between MTHFR C677T polymorphism and head and neck cancer (p = 0.50). Significant differences between the study and control groups were observed at age over 50 years, tobacco use, and male gender (p <0.001). There was no association of disease with clinical-histopathological parameters.
  
  
  CONCLUSIONS
  No association between the MTHFR C677T polymorphism and head and neck squamous cell carcinoma was possible in this study.
authors.affiliation: Centro Nacional de Desenvolvimento Científico e Tecnológico, Brazil.
authors.forename: Juliana Olsen
authors.initials: JO
authors.lastname: Rodrigues
authors.forename: Ana Lívia Silva
authors.initials: AL
authors.lastname: Galbiatti
authors.forename: Mariangela Torreglosa
authors.initials: MT
authors.lastname: Ruiz
authors.forename: Luiz Sergio
authors.initials: LS
authors.lastname: Raposo
authors.forename: José Victor
authors.initials: JV
authors.lastname: Maniglia
authors.forename: Erika Cristina
authors.initials: EC
authors.lastname: Pavarino-Bertelli
authors.forename: Eny Maria
authors.initials: EM
authors.lastname: Goloni-Bertollo
mesh_terms: D000367:Age Factors; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006258:Head and Neck Neoplasms / Q000209:etiology / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012189:Retrospective Studies; D012307:Risk Factors; D012737:Sex Factors
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)