title: RC-RNase-induced cell death in estrogen receptor positive breast tumors through down-regulation of Bcl-2 and estrogen receptor.
abstract: RC-RNase exerts anti-cancer effects on many tumors. However, the mechanisms by which RC-RNase induces cytotoxicity in different tumor cells are unclear. Currently, estrogen receptor (ER)-positive and negative breast tumors are treated with RC-RNase. Our data demonstrate that RC-RNase induces cell death on ER-positive but not on ER-negative breast tumors. This study also shows that down-regulation of ER and Bcl-2 is found on RC-RNase-treated ER-positive breast tumors. Additionally, Bcl-2 overxpression can prevent ER-positive breast tumors from cell death treated with RC-RNase. In summary, this study demonstrates that RC-RNase-induced cell death of ER-positive breast tumors is through regulation of ER and Bcl-2.
authors.affiliation: Department of Emergency Medicine, Buddhist Tzu-Chi University and General Hospital, Hualien and Taipei Branch, Taiwan.
authors.forename: Hsu-Hung
authors.initials: HH
authors.lastname: Tseng
authors.forename: Yung-Luen
authors.initials: YL
authors.lastname: Yu
authors.forename: Yi-Lin Sophia
authors.initials: YL
authors.lastname: Chen
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Chen
authors.forename: Chu-Lin
authors.initials: CL
authors.lastname: Chou
authors.forename: Tsun-Yung
authors.initials: TY
authors.lastname: Kuo
authors.forename: Jaang-Jiun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Ming-Chieh
authors.initials: MC
authors.lastname: Lee
authors.forename: Ting-Han
authors.initials: TH
authors.lastname: Huang
authors.forename: Mark Hung-Chih
authors.initials: MH
authors.lastname: Chen
authors.forename: Giou-Teng
authors.initials: GT
authors.lastname: Yiang
mesh_terms: D029845:Amphibian Proteins / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D004722:Endoribonucleases / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019254:Genes, bcl-2 / Q000187:drug effects*; D006801:Humans; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D029845:Amphibian Proteins; D011960:Receptors, Estrogen; D004722:Endoribonucleases; C094067:pyrimidine-guanine sequence-specific ribonuclease, Rana catesbeiana; D053148:Caspase 3