title: [The advantages and disadvantages of GMS and PAS Staining in fungal keratitis].
abstract: OBJECTIVE
  To compare the advantages and disadvantages of GMS and PAS Staining in the diagnosis of fungal keratitis.
  
  
  METHODS
  Retrospectively analysed the sections of 102 cases of fungal keratitis from November 2007 to June 2010 in our department. Statistically analysed the positive rate of different statining methods, and summarized technical cruxes of GMS and PAS staining.
  
  
  RESULTS
  The positive rates of GMS and PAS staining for fungal keratitis were 100% and 93.14%, respectively.
  
  
  CONCLUSIONS
  GMS staining is better than PAS staining for fungal keratitis in displaying fungi. And it takes less time, is easier to operate and presents a more durable colour compared with PAS staining. But PAS staining is superior to GMS staining in displaying fungal septa and eliminating interference from pigment-containing cells and other cells.
authors.affiliation: State Key Laboratory of Ophthalmology,Pathology laboratory,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Zheng
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhang
authors.forename: Jianxian
authors.initials: J
authors.lastname: Lin
authors.forename: Yongping
authors.initials: Y
authors.lastname: Li
authors.forename: Yungang
authors.initials: Y
authors.lastname: Ding
authors.forename: Juan
authors.initials: J
authors.lastname: Yao
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D003320:Corneal Ulcer; D015821:Eye Infections, Fungal; D005658:Fungi; D006801:Humans; D012189:Retrospective Studies; D013194:Staining and Labeling