title: Genotyping rotavirus RNA from archived rotavirus-positive rapid test strips.
abstract: Genotyping circulating rotaviruses before and after introduction of rotavirus vaccine is useful for evaluating vaccine-associated changes in genotype distribution. We determined frequency of rotavirus genotypes among 61 rotavirus-positive children hospitalized in Israel during the 2005-06 rotavirus season. Accurate molecular epidemiologic data were recovered from affinity-concentrated rotavirus immobilized in rotavirus-positive bands from air-dried, diagnostic rotavirus rapid test strips (dipstick) stored at room temperature from 1 week to 5 years. G genotypes were identical for 21 paired dipsticks and suspensions, whereas dipsticks or suspensions detected an additional G genotype in 2 samples. RNA sequences from 7 pairs were identical. Phylogenetic analysis suggested previously unreported G2 sublineages and G9 lineages. The ease with which dipsticks can be stored at local facilities and transported to central reference laboratories can reverse increasing difficulties in obtaining geographically representative stool samples and expand surveillance to regions lacking adequate laboratory facilities.
authors.affiliation: Central Virology Laboratory, Public Health Services, Israel Ministry of Health, Chaim Sheba Medical Center, Tel Hashomer, Israel. lester.shulman@sheba.health.gov.il
authors.forename: Lester M
authors.initials: LM
authors.lastname: Shulman
authors.forename: Ilana
authors.initials: I
authors.lastname: Silberstein
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Alfandari
authors.forename: Ella
authors.initials: E
authors.lastname: Mendelson
mesh_terms: D002648:Child; D002845:Chromatography / Q000379:methods; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D005838:Genotype; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007557:Israel / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D011934:Reagent Strips; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors
chemical_list: D012367:RNA, Viral; D011934:Reagent Strips