title: [Metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide].
abstract: OBJECTIVE
  To investigate the metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide.
  
  
  METHODS
  Gossypetin-8-O-beta-D-glucuronide was administrated orally at the dose of 250 mg x kg(-1) to healthy rats, then the urine was collected, pre-treated appropriately and detected by HPLC-DAD-MS(n).
  
  
  RESULTS
  In the rat urine, gossypetin-8-O-beta-D-glucuronide, along with 6 metabolites, were detected, and their structures were elucidated on the basis of reference standards, UV and MS(n) spectra. The metabolic pathways of gossypetin-8-O-beta-D-glucuronide included mainly deglycosylation, glucuronidation and methylation.
  
  
  CONCLUSIONS
  A rapid and sensitive method for the analysis of metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide was established and this will be useful for the further metabolism study of gossypetin-8-O-beta-D-glucuronide.
authors.affiliation: Department of Natural Medicines and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China. xutong821124@163.com
authors.forename: Tongtong
authors.initials: T
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qingying
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000008:administration & dosage / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005419:Flavonoids; C059922:gossypetin