title: Mice brain nitric oxide synthase response induced by anaesthetics and other porphyrinogenic drugs.
abstract: Porphyrias neuropathophysiology could be related to low levels of heme as a cofactor for nitric oxide synthase (NOS). We examined how anaesthetics and other porphyrinogenic agents affect mice NOS activity and expression. Brain response was differential depending on the cellular fraction analyzed. Most of the drugs diminished cytosolic activity. Instead, isoflurane, enflurane and ethanol increased mitochondrial activity. NOS expression also depended on the drug tested. A comparative study was performed in liver. Our present and previous results indicate the widespread action of porphyrinogenic agents in brain, which could be the reason why it is difficult to establish the onset of acute porphyria neurological manifestations.
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET, Hospital de Clínicas José de San Martín, University of Buenos Aires, Argentina.
authors.forename: Jimena
authors.initials: J
authors.lastname: Lavandera
authors.forename: Alcira
authors.initials: A
authors.lastname: Batlle
authors.forename: Ana María
authors.initials: AM
authors.lastname: Buzaleh
mesh_terms: D000777:Anesthetics / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002458:Cell Fractionation; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004790:Enzyme Induction; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D011164:Porphyrias / Q000139:chemically induced / Q000201:enzymology*
chemical_list: D000777:Anesthetics; D019001:Nitric Oxide Synthase