title: The endoplasmic reticulum-associated degradation is necessary for plant salt tolerance.
abstract: Eukaryotic organisms have quality-control mechanisms that allow misfolded or unassembled proteins to be retained in the endoplasmic reticulum (ER) and subsequently degraded by ER-associated degradation (ERAD). The ERAD pathway is well studied in yeast and mammals; however, the biological functions of plant ERAD have not been reported. Through molecular and cellular biological approaches, we found that ERAD is necessary for plants to overcome salt stress. Upon salt treatment ubiquitinated proteins increased in plant cells, especially unfolded proteins that quickly accumulated in the ER and subsequently induced ER stress responses. Defect in HRD3A of the HRD1/HRD3 complex of the ERAD pathway resulted in alteration of the unfolded protein response (UPR), increased plant sensitivity to salt, and retention of ERAD substrates in plant cells. Furthermore, we demonstrated that Ca(2+) release from the ER is involved in the elevation of UPR and reactive oxygen species (ROS) participates the ERAD-related plant salt response pathway.
authors.affiliation: State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Cui
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Li
authors.forename: Bojiao
authors.initials: B
authors.lastname: Yin
authors.forename: Huawei
authors.initials: H
authors.lastname: Zhang
authors.forename: Baoying
authors.initials: B
authors.lastname: Lin
authors.forename: Yaorong
authors.initials: Y
authors.lastname: Wu
authors.forename: Ran
authors.initials: R
authors.lastname: Xia
authors.forename: Sanyuan
authors.initials: S
authors.lastname: Tang
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D055786:Gene Knockout Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D014415:Tunicamycin / Q000494:pharmacology; D054875:Ubiquitination; D056811:Unfolded Protein Response
chemical_list: D000900:Anti-Bacterial Agents; D029681:Arabidopsis Proteins; C561098:HRD3A protein, Arabidopsis; D008565:Membrane Proteins; D018832:Molecular Chaperones; D017382:Reactive Oxygen Species; D014415:Tunicamycin; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis