title: Phosphodiesterase10A: abundance and circadian regulation in the retina and photoreceptor of the rat.
abstract: Phosphodiesterase10A (PDE10A) is a dual specific cyclic nucleotide phosphodiesterase that is specifically enriched in striatum and which has gained attention as a therapeutic target for psychiatric disorders. The present study shows that PDE10A is also highly expressed in retinal neurons including photoreceptors. The levels of PDE10A transcript and protein display daily rhythms which could be seen in preparations of the whole retina. Corresponding changes in PDE10A mRNA were seen in photoreceptors isolated using laser microdissection. This suggests that the expressional control of the photoreceptor Pde10a gene contributes to the observed cyclicity in the amount of retinal PDE10A. The daily rhythmicity in the retinal PDE10A mRNA amount is retained under constant darkness but can be blocked by constant light or modulated by the lighting regime. It therefore appears to be driven by the endogenous retinal clock system which itself is entrained by light. The findings presented place PDE10A in the context of the visual system and suggest a role of PDE10A in the adaptation of cyclic nucleotide signaling to daily changes in light intensity in retinal neurons including photoreceptors.
authors.affiliation: Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University, Saarstraße 19-21, 55099 Mainz, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Wolloscheck
authors.forename: Isabella
authors.initials: I
authors.lastname: Spiwoks-Becker
authors.forename: Oliver
authors.initials: O
authors.lastname: Rickes
authors.forename: Heike
authors.initials: H
authors.lastname: Holthues
authors.forename: Rainer
authors.initials: R
authors.lastname: Spessert
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002940:Circadian Rhythm / Q000502:physiology*; D047468:Immunoprecipitation; D007834:Lasers; D042282:Microdissection; D018613:Microscopy, Confocal; D009712:Nucleotides, Cyclic / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis*; D020419:Photoreceptor Cells, Vertebrate / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009712:Nucleotides, Cyclic; D012333:RNA, Messenger; C512064:PDE10A protein, rat; D010727:Phosphoric Diester Hydrolases