title: Evaluation of superoxide dismutase from Helicobacter pylori as a protective vaccine antigen.
abstract: Helicobacter pylori, the major cause of gastric cancer, have mechanisms that allow colonization of the inhospitable gastric mucosa, including enzymes such as superoxide dismutase (SOD) which protect against reactive oxygen species. As SOD is essential for in vivo colonization, we theorized it might constitute a viable vaccine target. H. pylori SOD was expressed in E. coli and a purified recombinant protein used to vaccinate mice, prior to live H. pylori challenge. Partial protective immunity was induced, similar to that commonly observed with other antigens tested previously. This suggests SOD may have utility in a combination vaccine comprising several protective antigens.
authors.affiliation: Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Alison L
authors.initials: AL
authors.lastname: Every
authors.forename: Andrew
authors.initials: A
authors.lastname: Stent
authors.forename: Margaret B
authors.initials: MB
authors.lastname: Moloney
authors.forename: Garrett Z
authors.initials: GZ
authors.lastname: Ng
authors.forename: Caroline D
authors.initials: CD
authors.lastname: Skene
authors.forename: Stirling J
authors.initials: SJ
authors.lastname: Edwards
authors.forename: Philip
authors.initials: P
authors.lastname: Sutton
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005260:Female; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000276:immunology; D056724:Immunity, Humoral; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D013482:Superoxide Dismutase