title: Subinhibitory concentrations of farrerol reduce α-toxin expression in Staphylococcus aureus.
abstract: In this study, the antibacterial activity of farrerol against Staphylococcus aureus was determined. The minimum inhibitory concentrations capable of inhibiting 35 S. aureus strains ranged from 4 to 16 μg mL(-1) . A haemolysis assay, Western blot and real-time reverse transcriptase-PCR assay were performed to identify the influence of subinhibitory concentrations of farrerol on the secretion of α-toxin by S. aureus. The results show that farrerol significantly decreased, in a dose-dependent manner, the production of α-toxin by both methicillin-sensitive S. aureus and methicillin-resistant S. aureus.
authors.affiliation: Institute of Zoonoses, College of Animal Sciences and Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Jiazhang
authors.initials: J
authors.lastname: Qiu
authors.forename: Hua
authors.initials: H
authors.lastname: Xiang
authors.forename: Chen
authors.initials: C
authors.lastname: Hu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Hongen
authors.initials: H
authors.lastname: Li
authors.forename: Mingjing
authors.initials: M
authors.lastname: Luo
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D015153:Blotting, Western; D002867:Chromones / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D002867:Chromones; D006460:Hemolysin Proteins; C021091:staphylococcal alpha-toxin; C015881:farrerol