title: [The inhibition of tamoxifen on sodium channel in SHG-44 glioma cell-line].
abstract: OBJECTIVE
  To explore the effect of tamoxifen on voltage-dependent sodium channels in SHG-44 glioma cell line.
  
  
  METHODS
  Whole-cell patch clamp technique was used to record the Na currents in SHG-44 cell line and to investigate the effect of tamoxifen of different concentration on this channel currents.
  
  
  RESULTS
  This channel activated and inactivated quickly. Tamoxifen could significantly decrease the amplitude of Na currents of SHG-44 cell line. This block effect was dose dependent and voltage dependent. When the holding potential was 0 mV, 8 micromol/L tamoxifen could block this currents 69%. The half inhibition concentration (IC50) was 5.54 micromol/L.
  
  
  CONCLUSIONS
  Tamoxifen could significantly block the voltage dependent sodium channel in malignant glioma cell line SHG-44. It might be one of the mechanisms that tamoxifen inhibit glioma proliferation. clamp technique was used to record the Na currents in SHG-44 cell line and to investigate the effect of tamoxifen of different concentration on this channel currents.
  
  
  RESULTS
  This channel activated and inactivated quickly. Tamoxifen could significantly decrease the amplitude of Na currents of SHG-44 cell line. This block effect was dose dependent and voltage dependent. When the holding potential was 0 mV, 8 micromol/L tamoxifen could block this currents 69%. The half inhibition concentration (IC50) was 5.54 micromol/L.
  
  
  CONCLUSIONS
  Tamoxifen could signifi-cantly block the voltage dependent sodium channel in malignant glioma cell line SHG-44. It might be one of the mechanisms that tamoxifen inhibit glioma proliferation.
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Jiao
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D018408:Patch-Clamp Techniques; D026941:Sodium Channel Blockers / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: D018931:Antineoplastic Agents, Hormonal; D026941:Sodium Channel Blockers; D013629:Tamoxifen