title: Molecular study of porcine transmissible gastroenteritis virus after serial animal passages revealed point mutations in S protein.
abstract: Porcine respiratory coronavirus is related genetically to porcine transmissible gastroenteritis virus with a large deletion in S protein. The respiratory virus is a mutated form that may be a consequence of the gastroenteritis virus's evolution. Intensive passages of the virus in its natural host may enhance the appearance of mutations and therefore may contribute to any attenuated form of the virus. The objective of this study was to characterize the porcine transmissible gastroenteritis virus TMK22 strain after passages in piglets from 1992 until 2007. A typical experimental infection, molecular characterization, and serological analysis were also carried out to further characterize and to evaluate any significant difference between strains. The sequence analysis showed two amino acid deletions and loss of an N-glycosylation site in transmissible gastroenteritis virus S protein after passages in piglets. Although these deletions were positioned at the beginning of the antigenic site B of S protein, no clinical differences were observed in piglets infected experimentally either with the native virus or the mutated one. Serological tests did not show any antibody reactivity difference between the two strains. In this article, we report that the S protein deletion did not affect the virus's pathogenicity. The variety of the virus's evolutionary forms may be a result, not only of the multiple passages in natural hosts, but also of other factors, such as different pathogens co-infection, nutrition, immunity, and others. Further studies need to be carried out to characterize the mutated strain.
authors.affiliation: Laboratory for Diagnosis of Porcine and Bovine Viral Diseases, Federal Centre for Animal Health, FGI ARRIAH, Vladimir, Yur'evets 600901, Russia. nabilabidbensalem2008@gmail.com
authors.forename: Nabil Ben Salem
authors.initials: NB
authors.lastname: Abid
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Chupin
authors.forename: Olga P
authors.initials: OP
authors.lastname: Bjadovskaya
authors.forename: Olga G
authors.initials: OG
authors.lastname: Andreeva
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.forename: Javier
authors.initials: J
authors.lastname: Buesa
authors.forename: Taufik Z
authors.initials: TZ
authors.lastname: Baybikov
authors.forename: Larisa B
authors.initials: LB
authors.lastname: Prokhvatilova
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D005761:Gastroenteritis, Transmissible, of Swine / Q000821:virology; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D012367:RNA, Viral; D017423:Sequence Analysis, RNA; D064370:Spike Glycoprotein, Coronavirus; D013552:Swine / Q000821:virology*; D005760:Transmissible gastroenteritis virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D008562:Membrane Glycoproteins; D012367:RNA, Viral; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus