title: IgG responses to tissue-associated antigens as biomarkers of immunological treatment efficacy.
abstract: We previously demonstrated that IgG responses to a panel of 126 prostate tissue-associated antigens are common in patients with prostate cancer. In the current report we questioned whether changes in IgG responses to this panel might be used as a measure of immune response, and potentially antigen spread, following prostate cancer-directed immune-active therapies. Sera were obtained from prostate cancer patients prior to and three months following treatment with androgen deprivation therapy (n = 34), a poxviral vaccine (n = 31), and a DNA vaccine (n = 21). Changes in IgG responses to individual antigens were identified by phage immunoblot. Patterns of IgG recognition following three months of treatment were evaluated using a machine-learned Bayesian Belief Network (ML-BBN). We found that different antigens were recognized following androgen deprivation compared with vaccine therapies. While the number of clinical responders was low in the vaccine-treated populations, we demonstrate that ML-BBN can be used to develop potentially predictive models.
authors.affiliation: Carbone Comprehensive Cancer Center, University of Wisconsin, 1111 Highland Avenue, Madison, WI 53705, USA.
authors.forename: Heath A
authors.initials: HA
authors.lastname: Smith
authors.forename: Brett B
authors.initials: BB
authors.lastname: Maricque
authors.forename: John
authors.initials: J
authors.lastname: Eberhardt
authors.forename: Benjamin
authors.initials: B
authors.lastname: Petersen
authors.forename: James L
authors.initials: JL
authors.lastname: Gulley
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Schlom
authors.forename: Douglas G
authors.initials: DG
authors.lastname: McNeel
mesh_terms: D000465:Algorithms; D000951:Antigens, Neoplasm / Q000276:immunology*; D001185:Artificial Intelligence; D001499:Bayes Theorem; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000276:immunology; D019295:Computational Biology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D008297:Male; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000628:therapy*; D015398:Signal Transduction; D016896:Treatment Outcome; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D019496:Cancer Vaccines; D007074:Immunoglobulin G; D019444:Vaccines, DNA