title: Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.
abstract: The genomic RNA of negative-strand RNA viruses, such as vesicular stomatitis virus (VSV), is completely enwrapped by the nucleocapsid protein (N) in every stage of virus infection. During viral transcription/replication, however, the genomic RNA in the nucleocapsid must be accessible by the virus-encoded RNA-dependent RNA polymerase in order to serve as the template for RNA synthesis. With the VSV nucleocapsid and a nucleocapsid-like particle (NLP) produced in Escherichia coli, we have found that the RNA in the VSV nucleocapsid can be removed by RNase A, in contrast to what was previously reported. Removal of the RNA did not disrupt the assembly of the N protein, resulting in an empty capsid. Polyribonucleotides were reencapsidated into the empty NLP, and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence.
authors.affiliation: Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA.
authors.forename: Todd J
authors.initials: TJ
authors.lastname: Green
authors.forename: Michael
authors.initials: M
authors.lastname: Rowse
authors.forename: Jun
authors.initials: J
authors.lastname: Tsao
authors.forename: Jungsoon
authors.initials: J
authors.lastname: Kang
authors.forename: Peng
authors.initials: P
authors.lastname: Ge
authors.forename: Z Hong
authors.initials: ZH
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
mesh_terms: D004926:Escherichia coli; D019251:Nucleocapsid / Q000502:physiology*; D019590:Nucleocapsid Proteins / Q000378:metabolism; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D018116:Vesiculovirus / Q000502:physiology*
chemical_list: D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D012259:Ribonuclease, Pancreatic