title: The effect of Ni(II) on properties of bulking activated sludge and microbial analysis of sludge using 16S rDNA gene.
abstract: This study investigated the effect of nickel on properties and microbial community of bulking activated sludge when 60-240 mg/L Ni(II) was dosed continuously in a sequencing batch reactor (SBR) over 350 days. Results showed that 120 mg/L nickel did not significantly inhibited removal of organic pollutant by activated sludge. However, the system was completely upset when 240 mg/L Ni(II) was dosed. Improvement of settling and dewatering ability was also observed with the addition of Ni(II). In addition, investigations by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA of bacteria strain demonstrated that Ni(II) significantly affected microbial community of bulking activated sludge, judging from the elimination of original species and emergence of possible new nickel-resistant bacteria. The effect of nickel on shift of microbial community was an important cause resulted in the improvement of sludge properties in this bulking activated sludge system.
authors.affiliation: Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Ruihua
authors.initials: R
authors.lastname: Dai
mesh_terms: D001419:Bacteria / Q000235:genetics; D001673:Biodegradation, Environmental; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D004275:DNA, Ribosomal / Q000235:genetics*; D058645:Denaturing Gradient Gel Electrophoresis; D004305:Dose-Response Relationship, Drug; D009532:Nickel / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D009532:Nickel; D009584:Nitrogen