title: [Effects of dexamethasone and vitamin B12 on expression of fibroblast growth factor 10 and fibroblast growth factor receptor 2b during early palatogenesis].
abstract: OBJECTIVE
  To observe the alteration of fibroblast growth factor 10 (Fgf10) and fibroblast growth factor receptor 2 (Fgfr2b) signal in mouse embryonic palate after dexamethasone and vitamin B12 exposure.
  
  
  METHODS
  Dams were divided teratogenetic group, antagomistic group and control group and were respectively injected dexamethasone, dexamethasone and vitamin B12, and normal sodium. Dams were killed and fetus was collected at embryo 12.5 and 13.5 day. The expression of Fgf10 and Fgfr2b and mesenchymal cells proliferation of mouse embryonic by western blotting and BrdU assay were checked.
  
  
  RESULTS
  Fgf10 and Fgfr2b expression was down-regulated and mesenchymal cells proliferation was inhibited significantly after dexamethasone exposure. After vitamin B12 treatment, Fgf10 and Fgfr2b expression did not restore, but cells proliferation was recovered.
  
  
  CONCLUSIONS
  Dexamethasone and vitamin B12 affected the expression of Fgf10 and Fgfr2b of mouse embryonic palate and mesenchyme cells proliferation, but the change was disaccord.
authors.affiliation: State Key Laboratory of Oral Diseases, Siehuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Sheng-jun
authors.initials: SJ
authors.lastname: Lu
authors.forename: Cheng-hao
authors.initials: CH
authors.lastname: Li
authors.forename: Jing-lin
authors.initials: JL
authors.lastname: Zhou
authors.forename: Tian
authors.initials: T
authors.lastname: Meng
authors.forename: Qian
authors.initials: Q
authors.lastname: Zheng
authors.forename: Bing
authors.initials: B
authors.lastname: Shi
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003907:Dexamethasone; D051526:Fibroblast Growth Factor 10; D051379:Mice; D051497:Receptor, Fibroblast Growth Factor, Type 2; D015398:Signal Transduction; D014805:Vitamin B 12
chemical_list: C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D003907:Dexamethasone; D051497:Receptor, Fibroblast Growth Factor, Type 2; D014805:Vitamin B 12