title: Gene expression profiling of subcutaneous adipose tissue in morbid obesity using a focused microarray: distinct expression of cell-cycle- and differentiation-related genes.
abstract: BACKGROUND
  Obesity results from an imbalance between food intake and energy expenditure, which leads to an excess of adipose tissue. The excess of adipose tissue and adipocyte dysfunction associated with obesity are linked to the abnormal regulation of adipogenesis. The objective of this study was to analyze the expression profile of cell-cycle- and lipid-metabolism-related genes of adipose tissue in morbid obesity.
  
  
  METHODS
  We used a custom-made focused cDNA microarray to determine the adipose tissue mRNA expression profile. Gene expression of subcutaneous abdominal fat samples from 15 morbidly obese women was compared with subcutaneous fat samples from 10 nonobese control patients. The findings were validated in an independent population of 31 obese women and 9 obese men and in an animal model of obesity (Lepob/ob mice) by real-time RT-PCR.
  
  
  RESULTS
  Microarray analysis revealed that transcription factors that regulate the first stages of adipocyte differentiation, such as CCAAT/enhancer binding protein beta (C/EBPβ) and JUN, were upregulated in the adipose tissues of morbidly obese patients. The expression of peroxisome proliferator-activated receptor gamma (PPARγ), a transcription factor which controls lipid metabolism and the final steps of preadipocyte conversion into mature adipocytes, was downregulated. The expression of three cyclin-dependent kinase inhibitors that regulate clonal expansion and postmitotic growth arrest during adipocyte differentiation was also altered in obese subjects: p18 and p27 were downregulated, and p21 was upregulated. Angiopoietin-like 4 (ANGPTL4), which regulates angiogenesis, lipid and glucose metabolism and it is know to increase dramatically in the early stages of adipocyte differentiation, was upregulated. The expression of C/EBPβ, p18, p21, JUN, and ANGPTL4 presented similar alterations in subcutaneous adipose tissue of Lepob/ob mice.
  
  
  CONCLUSIONS
  Our microarray gene profiling study revealed that the expression of genes involved in adipogenesis is profoundly altered in the subcutaneous adipose tissue of morbidly obese subjects. This expression pattern is consistent with an immature adipocyte phenotype that could reflect the expansion of the adipose tissue during obesity.
authors.affiliation: Servicio de Bioquímica-Investigación, Hospital Ramón y Cajal, IRYCIS, Madrid, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Rodríguez-Acebes
authors.forename: Nuria
authors.initials: N
authors.lastname: Palacios
authors.forename: José I
authors.initials: JI
authors.lastname: Botella-Carretero
authors.forename: Nuria
authors.initials: N
authors.lastname: Olea
authors.forename: Lorena
authors.initials: L
authors.lastname: Crespo
authors.forename: Roberto
authors.initials: R
authors.lastname: Peromingo
authors.forename: Diego
authors.initials: D
authors.lastname: Gómez-Coronado
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Lasunción
authors.forename: Clotilde
authors.initials: C
authors.lastname: Vázquez
authors.forename: Javier
authors.initials: J
authors.lastname: Martínez-Botas
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002453:Cell Cycle; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008820:Mice, Obese; D046228:Microarray Analysis; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis