title: Lipopolysaccharide enhances glutaric acid-induced seizure susceptibility in rat pups: behavioral and electroencephalographic approach.
abstract: Glutaric acidemia type I (GA-I) is an inherited metabolic disease characterized by accumulation of glutaric acid (GA) and seizures. Considering that seizures are precipitated by common infections in children with GA-I, we investigated whether lipopolysaccharide (LPS) modifies GA-induced electrographic and neurochemical alterations in 21 days-old rats. The effect of LPS on convulsive behavior and electroencephalographic (EEG) alterations induced by GA (0.13; 0.4; 1.3 μmol/striatum) was determined in freely moving rats. After EEG recordings, we measured the levels of interleukin 1β (IL-1β) in GA-injected striatum. The injection of LPS (2mg/kg; i.p.) 6h before of GA administration, reduced the latency and increased the duration of seizures induced by GA (1.3 μmol/site). In addition, LPS administration increased IL-1β striatal levels, which positively correlated with total time in seizures. The intrastriatal injection of an IL-1β antibody (200 ng/2 μl) prevented the facilitation of GA-induced seizures by LPS. These data suggest that inflammatory processes during critical periods of development may decrease GA-induced seizure threshold.
authors.affiliation: Centro de Ciências da Saúde, Laboratório de Psicofarmacologia e Neurotoxicidade, Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Danieli Valnes
authors.initials: DV
authors.lastname: Magni
authors.forename: Mauren Assis
authors.initials: MA
authors.lastname: Souza
authors.forename: Ana Paula Ferreira
authors.initials: AP
authors.lastname: Oliveira
authors.forename: Ana Flávia
authors.initials: AF
authors.lastname: Furian
authors.forename: Mauro Schneider
authors.initials: MS
authors.lastname: Oliveira
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
authors.forename: Adair Roberto Soares
authors.initials: AR
authors.lastname: Santos
authors.forename: Carlos Fernando
authors.initials: CF
authors.lastname: Mello
authors.forename: Luiz Fernando Freire
authors.initials: LF
authors.lastname: Royes
authors.forename: Michele Rechia
authors.initials: MR
authors.lastname: Fighera
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000032:analysis; D001522:Behavior, Animal / Q000187:drug effects*; D001831:Body Temperature / Q000187:drug effects; D001923:Brain Chemistry / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004567:Electrodes, Implanted; D004569:Electroencephalography / Q000187:drug effects*; D005977:Glutarates / Q000494:pharmacology*; D007267:Injections; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology* / Q000523:psychology
chemical_list: D000906:Antibodies; D005977:Glutarates; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; C035736:glutaric acid