title: Plasmid-mediated quinolone resistance in pseudomonas putida isolates from imported shrimp.
abstract: Fourteen quinolone-resistant Pseudomonas putida isolates were recovered from imported frozen shrimp sold in the United States. Two isolates harbored plasmids with qnrA and qnrB genes. PCR and DNA sequencing of quinolone resistance-determining regions identified novel substitutions in GyrA (His139→Glu and Thr128→Ala) and GyrB (Thr442→Asn, Gly470→Ala, and Ile487→Pro) and previously reported substitutions in GyrB (Asp489→Glu) and ParC (Thr105→Pro).
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA. quynhtien.n.tran@gmail.com
authors.forename: Quynh T
authors.initials: QT
authors.lastname: Tran
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Nawaz
authors.forename: Joanna
authors.initials: J
authors.lastname: Deck
authors.forename: Kiet T
authors.initials: KT
authors.lastname: Nguyen
authors.forename: Carl E
authors.initials: CE
authors.lastname: Cerniglia
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D027101:DNA Topoisomerase IV / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D020125:Mutation, Missense; D010957:Plasmids; D016133:Polymerase Chain Reaction; D016958:Pseudomonas putida / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D015363:Quinolones / Q000494:pharmacology*; D017747:Seafood / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014481:United States
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015363:Quinolones; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase