title: Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
abstract: Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.
authors.affiliation: Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808, USA.
authors.forename: Pedro P
authors.initials: PP
authors.lastname: Carvalho
authors.forename: Xiying
authors.initials: X
authors.lastname: Wu
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.forename: Marilyn
authors.initials: M
authors.lastname: Dietrich
authors.forename: Isabel R
authors.initials: IR
authors.lastname: Dias
authors.forename: Manuela E
authors.initials: ME
authors.lastname: Gomes
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000328:Adult; D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008875:Middle Aged; D011994:Recombinant Proteins / Q000737:chemistry*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D013552:Swine; D014357:Trypsin / Q000737:chemistry*; D055815:Young Adult
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D014357:Trypsin