title: Development of a fully automated toxicological LC-MS(n) screening system in urine using online extraction with turbulent flow chromatography.
abstract: In clinical toxicology, fast and specific methods are necessary for the screening of different classes of drugs. Therefore, an online extraction high-performance liquid chromatography coupled to mass spectrometry (LC-MS(n)) screening method using a MS(2) and MS(3) spectral library for the identification of xenobiotic substances has been developed and validated. Samples were run twice, once native and once after enzymatic hydrolysis. Internal standards and buffer were added to the urine samples. Following centrifugation, the supernatant was injected into the system. Extraction was performed by online turbulent flow chromatography. The chromatographic separation was achieved using a Phenyl/Hexyl column. For detection, a linear ion trap, equipped with an APCI interface, was used and the different compounds were identified using a MS(2) and MS(3) spectral library containing 356 compounds. The turnaround time to report the results of the screening including hydrolysis was approximately 2 h. About 92% of the 356 substances could be identified with a limit of identification below 100 ng/ml. The recovery and matrix effect experiments showed suitable results, and in six drug-free urine samples of healthy volunteers analyzed for selectivity, no substances have been identified. Carryover could be well controlled, and the method had a good reproducibility. The comparison of the results of 103 real patient urine samples showed a good agreement between the existing GC-MS and LC-MS methods with offline extraction and the new online extraction LC-MS(n) screening method. The presented method allows a fast and sensitive analysis of a broad range of compounds.
authors.affiliation: Institute for Clinical Chemistry, University Hospital Zurich, Raemistrasse 100, 8091 Zurich, Switzerland.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Mueller
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Duretz
authors.forename: Francois A
authors.initials: FA
authors.lastname: Espourteille
authors.forename: Katharina M
authors.initials: KM
authors.lastname: Rentsch
mesh_terms: D000465:Algorithms; D001331:Automation; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000652:urine*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations