title: Adiponectin inhibits lymphotoxin-β receptor-mediated NF-κB signaling in human umbilical vein endothelial cells.
abstract: Adiponectin exerts anti-diabetic and anti-atherogenesis properties through its 2 receptors (AdipoR1 and AdipoR2). However, the signaling pathways responsible for the anti-inflammatory effects of adiponectin are largely unknown. In this study, we identified the lymphotoxin (LT)-β receptor (LTBR) as an interacting partner of human AdipoR1 by using a yeast two-hybrid screening. The interaction between LTBR and AdipoR1 was confirmed by co-immunoprecipitation and co-localization analysis. Furthermore, adiponectin incubation inhibited lymphotoxin-induced NF-κB activation and the expression of adhesion molecules in human umbilical vein endothelial cells. These results indicated that AdipoR1 interacted with LTBR and mediated the inhibition of LTBR-activated NF-κB pathway.
authors.affiliation: Department of Cardiology, Hangzhou the First People's Hospital, Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China. xuyizhou5@gmail.com
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Xu
authors.forename: Chu
authors.initials: C
authors.lastname: Zhang
authors.forename: Ningfu
authors.initials: N
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Ling
authors.forename: Peizhang
authors.initials: P
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D053361:Lymphotoxin alpha1, beta2 Heterotrimer / Q000378:metabolism; D053259:Lymphotoxin beta Receptor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014471:Umbilical Veins / Q000166:cytology
chemical_list: C475418:ADIPOR1 protein, human; D052242:Adiponectin; D053361:Lymphotoxin alpha1, beta2 Heterotrimer; D053259:Lymphotoxin beta Receptor; D016328:NF-kappa B; D054419:Receptors, Adiponectin