title: Multifunctional hollow nanoparticles based on graft-diblock copolymers for doxorubicin delivery.
abstract: This article reports a flexible hollow nanoparticles, self-assembling from poly(N-vinylimidazole-co-N-vinylpyrrolidone)-g-poly(d,l-lactide) graft copolymers and methoxyl/functionalized-PEG-PLA diblock copolymers, as an anticancer drug doxorubicin (Dox) carrier for cancer targeting, imaging, and cancer therapy. This multifunctional hollow nanoparticle exhibited a specific on-off switch drug release behavior, owning to the pH-sensitive structure of imidazole, to release Dox in acidic surroundings (intracellular endosomes) and to capsulate Dox in neutral surroundings (blood circulation or extracellular matrix). Imaging by SPECT/CT shows that nanoparticle conjugated with folic acids ensures a high intratumoral accumulation due to the folate-binding protein (FBP)-binding effect. In vivo tumor growth inhibition shows that nanoparticles exhibited excellent antitumor activity and a high rate of apoptosis in cancer cells. After 80-day treatment course of nanoparticles, it did not appreciably cause heart, liver and kidney damage by inactive Dox or polymeric materials. The results indicate that the flexible carriers with an on-off switched drug release may be allowed to accurately deliver to targeted tumors for cancer therapy.
authors.affiliation: Department of Nuclear Medicine and Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan 333, ROC.
authors.forename: Pei-Lin
authors.initials: PL
authors.lastname: Lu
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Chen
authors.forename: Ta-Wei
authors.initials: TW
authors.lastname: Ou
authors.forename: Hung-Hao
authors.initials: HH
authors.lastname: Chen
authors.forename: Hsieh-Chih
authors.initials: HC
authors.lastname: Tsai
authors.forename: Chih-Jen
authors.initials: CJ
authors.lastname: Wen
authors.forename: Chun-Liang
authors.initials: CL
authors.lastname: Lo
authors.forename: Shiaw-Pyng
authors.initials: SP
authors.lastname: Wey
authors.forename: Kun-Ju
authors.initials: KJ
authors.lastname: Lin
authors.forename: Tzu-Chen
authors.initials: TC
authors.lastname: Yen
authors.forename: Ging-Ho
authors.initials: GH
authors.lastname: Hsiue
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D001672:Biocompatible Materials / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D011108:Polymers / Q000737:chemistry*; D011897:Random Allocation; D014018:Tissue Distribution
chemical_list: D000903:Antibiotics, Antineoplastic; D001672:Biocompatible Materials; D011108:Polymers; D004317:Doxorubicin