title: Two-photon spectral imaging with high temporal and spectral resolution.
abstract: We introduce a fast spectral imaging system using an electron-multiplying charge-coupled device (EM-CCD) as a detector. Our system is combined with a custom-built two-photon excitation laser scanning microscope and has 80 detection channels, which allow for high spectral resolution and fast frame acquisition without any loss of spectral information. To demonstrate the efficiency of our approach, we applied this technology to monitor fluorescent proteins and quantum dot-labeled G protein-coupled receptors in living cells as well as autofluorescence in tissue samples.
authors.affiliation: Neurodegeneration and Applied Microscopy, Institut Pasteur Korea, Seongnam, Gyeonggi-do, Korea.
authors.forename: Kang-Bin
authors.initials: KB
authors.lastname: Im
authors.forename: Moon-Sik
authors.initials: MS
authors.lastname: Kang
authors.forename: Jiho
authors.initials: J
authors.lastname: Kim
authors.forename: Felix
authors.initials: F
authors.lastname: Bestvater
authors.forename: Zahir
authors.initials: Z
authors.lastname: Seghiri
authors.forename: Malte
authors.initials: M
authors.lastname: Wachsmuth
authors.forename: Regis
authors.initials: R
authors.lastname: Grailhe
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*