title: Overestimation of incidence of hepatitis B virus mixed-genotype infections by use of the new line probe INNO-LiPA genotyping assay.
abstract: The new version of the INNO-LiPA HBV genotyping assay (Innogenetics) developed to identify all hepatitis B virus (HBV) genotypes, A to H, has been evaluated in comparison with sequencing of PCR-amplified HBV DNA from 200 samples before or after cloning. The genotyping data obtained with INNO-LiPA were in agreement with those from direct sequencing in the 179 samples characterized by the two methods. INNO-LiPA revealed 28 mixed infections. However, sequencing after molecular cloning confirmed only 15 of them and did not identify any that were of genotype H (n = 9). Our study demonstrates that INNO-LiPA overestimates mixed infections as a result of erroneous genotype H detection.
authors.affiliation: National Reference Center for Hepatitis B and C in Blood Transfusion, Institut National de la Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Mercier
authors.forename: Syria
authors.initials: S
authors.lastname: Laperche
authors.forename: Annie
authors.initials: A
authors.lastname: Girault
authors.forename: Camille
authors.initials: C
authors.lastname: Sureau
authors.forename: Annabelle
authors.initials: A
authors.lastname: Servant-Delmas
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D003951:Diagnostic Errors; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology* / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D015994:Incidence; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D014773:Virology / Q000379:methods*