title: Nuclear proteins: finding and binding target sites in chromatin.
abstract: Fluorescent protein labelling, as well as impressive progress in live cell imaging have revolutionised the view on how essential nuclear functions like gene transcription regulation and DNA repair are organised. Here, we address questions like how DNA-interacting molecules find and bind their target sequences in the vast amount of DNA. In addition, we discuss methods that have been developed for quantitative analysis of data from fluorescence recovery after photobleaching experiments (FRAP).
authors.affiliation: Department of Pathology, Josephine Nefkens Institute, Erasmus University Medical Center, PO-Box 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: Martin E
authors.initials: ME
authors.lastname: van Royen
authors.forename: Angelika
authors.initials: A
authors.lastname: Zotter
authors.forename: Shehu M
authors.initials: SM
authors.lastname: Ibrahim
authors.forename: Bart
authors.initials: B
authors.lastname: Geverts
authors.forename: Adriaan B
authors.initials: AB
authors.lastname: Houtsmuller
mesh_terms: D001665:Binding Sites; D002843:Chromatin / Q000378:metabolism*; D004260:DNA Repair; D004058:Diffusion; D005069:Evaluation Studies as Topic; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002843:Chromatin; D009687:Nuclear Proteins; D049452:Green Fluorescent Proteins