title: [Screening and application of prokaryotic enhancer-like sequence 3A].
abstract: OBJECTIVE
  To screen enhancer-like sequences from Escherichia coli strain C600 genome, to construct an expression vector harboring prokaryotic enhancer-like sequence and study the effect of interferon gene expression.
  
  
  METHODS
  Enhancer-like element from Escherichia coli strain C600 genome was obtained by using the chloramphenicol acetyl-transferase (CAT) gene as reporter gene. An expression vector harboring prokaryotic enhancer-like sequence from Escherichia coli strain C600 was constructed. Interferon was expressed and assayed.
  
  
  RESULTS
  An enhancer-like sequences with distance and orientation independence property were screened and named 3A. Quantification test showed that the direct and reverse orientation of 3A could increase the activity of beta-galactosidase with 7.11 and 2.93 times. The enhancing activity of the element was on transcription level. An expression vector harboring the prokaryotic enhancer-like sequence 3P3 which was enhancing function region of sequence 3A was constructed. Using this vector the antiviral activity of interferon alpha-2b was increased by 3.7 times in comparison with the original expression plasmid.
  
  
  CONCLUSIONS
  3A enhancer-like sequence was screened from Escherichia coli strain C600 genome. Interferon gene was highly expressed by using an expression vector harboring enhancer-like sequences.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: He
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Liao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Wu
mesh_terms: D004742:Enhancer Elements, Genetic / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects* / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000737:chemistry; D007372:Interferons / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011387:Prokaryotic Cells; D012689:Sequence Homology, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D007372:Interferons; D001616:beta-Galactosidase