title: Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1.
abstract: The protein elicitor from the mycelium of Alternaria tenuissima has been isolated. The elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species, but without causing hypersensitive necrosis. The elicitor is reported to impart resistance against Verticillium dahliae and to increase yield in cotton, but its mechanism is not yet clear. In this study, the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of protein expression induced by PeaT1 in Arabidopsis. A significant difference in the relative abundances for the expression of different proteins related to metabolism, modification, regulatory, defense, stress and antioxidation was found in Arabidopsis.
authors.affiliation: Key Laboratory for Biological Control of the Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Guangyue
authors.initials: G
authors.lastname: Li
authors.forename: Xiufen
authors.initials: X
authors.lastname: Yang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zeng
authors.forename: Jianjun
authors.initials: J
authors.lastname: Mao
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dewen
authors.initials: D
authors.lastname: Qiu
mesh_terms: D000528:Alternaria / Q000737:chemistry* / Q000472:pathogenicity; D000818:Animals; D017360:Arabidopsis / Q000737:chemistry* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis*; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009181:Mycoses / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology
chemical_list: D029681:Arabidopsis Proteins; D005656:Fungal Proteins