title: Matrix metalloproteinases and membrane damage markers in sera of patients with acute myocardial infarction.
abstract: Coronary artery disease is a multifunctional disease and represents one of the leading causes of death worldwide. Oxidative stress appears as an etiological factor for myocardial damage during acute myocardial infarction. Some data suggest that acute coronary syndromes may also be influenced by matrix metalloproteinases through degradation of the fibrous cap of vulnerable atherosclerotic lesions. It has been indicated that gelatinases A and B play a key role in acute myocardial infarction and deoxyribonuclease I has been postulated to be a novel early phase marker of disease. The aim was to study activity of gelatinases A and B in acute myocardial infarction and its association with some membrane damage markers. Seventy-five patients with disease and seventy-five healthy controls were enrolled. Activities of lactate dehydrogenase, malate dehydrogenase, and deoxyribonuclease I were estimated using standard spectrophotometric assay and isoforms of lactate and malate dehydrogenases were determined using direct zymography. Activity of dehydrogenases was significantly higher in patients, while deoxyribonuclease I was lower. Isoform 2 of lactate dehydrogenase was significantly higher in the patient group. Gelatinases A and B were detected only in patients group. The results suggest determination of serum malate dehydrogenase activity to be used as an additional parameter for acute myocardial infarction diagnosis. Those findings suggest important role of gelatinases A and B as biomarkers of early stage of acute myocardial infarction together with membrane damage parameters.
authors.affiliation: Department of Medical Chemistry, University of Belgrade, Belgrade, Serbia. kristinagopcevic@yahoo.com
authors.forename: Kristina
authors.initials: K
authors.lastname: Gopcevic
authors.forename: Branislav
authors.initials: B
authors.lastname: Rovcanin
authors.forename: Dusan
authors.initials: D
authors.lastname: Kekic
authors.forename: Sandra
authors.initials: S
authors.lastname: Radenkovic
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood* / Q000378:metabolism; D016022:Case-Control Studies; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology*; D003850:Deoxyribonuclease I / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000097:blood / Q000378:metabolism; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis*; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D003850:Deoxyribonuclease I; D020782:Matrix Metalloproteinases