title: Effects of probiotic Escherichia coli Nissle 1917 on expression of cytochromes P450 along the gastrointestinal tract of male rats.
abstract: OBJECTIVE
  The aim of the study was to find whether probiotic Escherichia coli Nissle 1917 O6:K5:H1 (EcN) influences the expression of cytochromes P450 (CYP) in the rat intestine.
  
  
  METHODS
  Live bacterial suspension of EcN was administered to healthy male Wistar rats daily for 7 days. Control group of rats was stressed by oral application of the saline solution daily for 7 days as well. Sections of the duodenum, jejunum, ileum, caecum and colon have been taken from each experimental animal. With all individual samples, microsomal fraction has been prepared and expression of selected CYPs was determined by Western blotting. The levels of expression of CYPs were also evaluated by mRNA using real-time PCR.
  
  
  RESULTS
  It was found that there are changes in expression of CYP enzymes studied along the intestine. CYP1A1, 2B1/2 and 2E1 are present mainly in the duodenum and jejunum; on the other hand, CYP2C6 is expressed mainly in the caecum and colon. CYP3A was found all over the rat intestine. The results show that there are no prominent differences between control samples and samples with EcN, only the expression of CYP3A protein in the duodenum appears to exhibit a clear tendency to decrease. In the case of the colon, a significant increase in the expression of CYP3A (most likely CYP3A1) after treatment of rats with EcN was found.
  
  
  CONCLUSIONS
  This in vivo study revealed that the levels of colon CYP3A could be significantly increased in rats treated with probiotic EcN. On the contrary, the expression of CYP3A in the duodenum decreased. However, the changes in the expression of CYP enzymes are probably not as extensive to be clinically important in man; hence, most likely the probiotic EcN has little influence on the intestinal drug metabolism by CYP enzymes.
authors.affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Matuskova
authors.forename: Alena
authors.initials: A
authors.lastname: Tunkova
authors.forename: Eva
authors.initials: E
authors.lastname: Anzenbacherova
authors.forename: Rostislav
authors.initials: R
authors.lastname: Vecera
authors.forename: Michal
authors.initials: M
authors.lastname: Siller
authors.forename: Helena
authors.initials: H
authors.lastname: Tlaskalova-Hogenova
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Zidek
authors.forename: Pavel
authors.initials: P
authors.lastname: Anzenbacher
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D003106:Colon / Q000201:enzymology; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004386:Duodenum / Q000201:enzymology; D004926:Escherichia coli; D041981:Gastrointestinal Tract / Q000201:enzymology*; D008297:Male; D023421:Models, Animal; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C465138:Cyp3a23-3a1 protein, rat; D051544:Cytochrome P-450 CYP3A