title: [Study on the changes of the T-lymphocyte subsets and their significance in influenza A (H1N1) virus patients].
abstract: OBJECTIVE
  To study the changes of T-Lymphocyte and activated T-Lymphocyte subsets in influenza A (H1N1) virus patients.
  
  
  METHODS
  The percentages of the subsets of Lymphocyte were detected by flow cytometry in influenza A (H1N1) virus patients (n = 144) and normal controls (n = 41). Furthermore, the subsets of T-Lymphocyte and activated T-lymphocyte were analyzed in 83 among those patients before and after treatment.
  
  
  RESULTS
  Compared with the control group, the counts of Lymphocyte in patients with influenza A (H1N1) virus was significantly discreased, the counts of Lymphocyte in patients with influenza A virus concurrent pneumonia was significantly discreased those of no concurrent pneumonia; Compared with the control group, the percentage of T-lymphocyte in patients with influenza A virus concurrent pneumonia was significantly discreased. The counts and percentage of CD3 and CD8 cells was significantly discreased in patients (n = 83) before treatment; The counts of CD4 cells was significantly discreased before treatment. The percentage of HLA-DR+ CD+, HLA-DR+ CD4+ and HLA-DR+ CD8+ cells was significantly discreased in patients (n = 83) before treatment.
  
  
  CONCLUSIONS
  To understand the expression of the T-Lymphocyte and activated T-Lymphocyte subsets in influenza A (H1N1) virus patients may help to evaluate the patients' cellular immune status, but also be a guideline of early diagnosis of Influenza A (H1N1) virus.
authors.affiliation: Open Laboratory of Hangzhou Sixth People's Hospital, Hangzhou 310014, China.
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Zhang
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Zhu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Lou
mesh_terms: D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D005434:Flow Cytometry; D006684:HLA-DR Antigens; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000472:pathogenicity*; D009980:Influenza A virus; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000473:pathology*; D008213:Lymphocyte Activation / Q000276:immunology*; D018655:Lymphocyte Count; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D016827:CD8 Antigens; D006684:HLA-DR Antigens; D007252:Influenza Vaccines