title: [VEGF165-induced angiogenesis by regulating intracellular free Mg2+ in HUVECs].
abstract: OBJECTIVE
  The mechanism of vascular endothelial growth factor165 (VEGF165) on intracellular free magnesium ([Mg2+]i) in human umbilical vein endothelial cells (HUVECs) was investigated.
  
  
  METHODS
  [Mg2+]i in HUVECs loaded with fluorescent magnesium indicator mag-fura-2 were quantitatively detected the use of intracellular cation measurement system.
  
  
  RESULTS
  VEGF165 significantly increased [Mg2+]i in the extracellular Mg2+ and this effect could be blocked by pretreatment with tyrosine kinase inhibitors (tyrphostin A23 and genistein), phosphatidylinositol 3-kinase (PI3K) inhibitors (wortmannin and LY294002) and phospholipase Cgamma (PLCgamma) inhibitor (U73122). In contrast, phospholipase Cgamma (PLCgamma) inhibitor analog (U73343), mitogen-activated protein kinase inhibitors (SB202190 and PD98059) had no effect on the VEGF165-induced [Mg2+]i increase.
  
  
  CONCLUSIONS
  The increase of [Mg2+]i by VEGF165 originates from intracellular Mg2+ pool through tyrosine kinase/ PI3K/PLCgamma-dependent signaling pathways.
authors.affiliation: Department of Cardiology, Dalian University Medical School, Dalian 116021, China. hongbingzhe@hotmail.com
authors.forename: Bing-Zhe
authors.initials: BZ
authors.lastname: Hong
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Sheng-Fan
authors.initials: SF
authors.lastname: Li
authors.forename: Hai-Nan
authors.initials: HN
authors.lastname: Piao
authors.forename: Li-Jian
authors.initials: LJ
authors.lastname: Gao
authors.forename: Wan-Qiu
authors.initials: WQ
authors.lastname: Li
authors.forename: Ping-An
authors.initials: PA
authors.lastname: Cao
mesh_terms: D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008274:Magnesium / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051966:Phospholipase C gamma / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D019869:Phosphatidylinositol 3-Kinases; D011505:Protein-Tyrosine Kinases; D051966:Phospholipase C gamma; D008274:Magnesium