title: SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome.
abstract: We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.
authors.affiliation: Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
authors.forename: Ryan W
authors.initials: RW
authors.lastname: Benson
authors.forename: Tiziana M
authors.initials: TM
authors.lastname: Cafarelli
authors.forename: Veronica G
authors.initials: VG
authors.lastname: Godoy
mesh_terms: D019677:Artificial Gene Fusion; D002874:Chromosome Mapping / Q000379:methods*; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D050505:Mutant Proteins / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic
chemical_list: D029968:Escherichia coli Proteins; D050505:Mutant Proteins