title: [In vitro expansion of T cells stimulated by combination of IL-2, IL-7 and IL-15].
abstract: The aim of this study was to compare cell proliferation and function of the T cells acquired under various culture conditions for establishing a simple, safe and efficient cell expansion protocol in vitro. The peripheral blood mononuclear cells (PBMNC) were isolated and stimulated with autologous dendritic cells (DC) and EBV-transformed B lymphoblastoid cell line (BLCL) weekly. The cell proliferation test, flow cytometry with PI and Annexin V double staining, Cr release test and ELISPOT test were used to detect the cell expansion level, frequency of IFN-γ producing T cells, killing activity of antigen-specific T cells, cell apoptotic status and cell differentiation potential, respectively. The results indicated that use of IL-2 combined with IL-7 and IL-15 resulted in the highest cell expansion comparing to the use of IL-2 alone and the use of CD3/28 Microbeads. Also the cells obtained under cultivating with IL-2, IL-7 and IL-15 together showed high frequency of IFN-γ producing cells, strong killing activity, high viability and high differentiation potential with large portion of CD3(+)CD8(+) population among the T cells. It is concluded that a protocol is established in which the use of IL-2 combined with IL-7 and IL-15 induces the biggest cell expansion, expanded cells show high viability, strong differentiation potential, high frequency of IFN-γ producing cells and strong killing activity.
authors.affiliation: Department of Urology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.forename: Su-Xia
authors.initials: SX
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Jiang-Ping
authors.initials: JP
authors.lastname: Gao
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D015851:Interleukin-7 / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D019409:Interleukin-15; D007376:Interleukin-2; D015851:Interleukin-7