title: [Experimental study on inhibition of restenosis by osteopontin oligopeptide antagonist after de-endothelium].
abstract: OBJECTIVE
  Osteopontin 13-peptide(Gly158-Lys170), containing multi-function domains was used to inhibit the VSMC adhesion, migration. The mechanism of 13-peptide inhibiting neointima formation was investigated.
  
  
  METHODS
  The effect of 13-peptide on VSMC adhesion was tested by adhesion assay. The restenosis model was prepared balloon injury after administration of 13-peptide for 1 h, and then the 13-peptide was given by an intravenous drip for 7 days. The expression changes of OPN, FAK, ILK in vessel wall were detected by immunohistochemistry and Western blot.
  
  
  RESULTS
  The 13-peptide dose-dependently reduced adhesion of VSMC in OPN matrix, and the infiltration of macrophage in vessel wall also was reduced in the treatment group after balloon injury. The expression of OPN, FAK, ILK was down-regulated following with the inhibition of neointima thickening.
  
  
  CONCLUSIONS
  The OPN 13-peptide can inhibit inflammation and neointima formation by blocking the binding of OPN to it's receptors.
authors.affiliation: Hebei Laboratory of Medical Biotechnology, Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.forename: Jin-kun
authors.initials: JK
authors.lastname: Wen
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053495:Osteopontin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017539:Tunica Intima / Q000473:pathology
chemical_list: D053495:Osteopontin