title: Cancer/Testis antigen expression on mesenchymal stem cells isolated from different tissues.
abstract: OBJECTIVE
  The expression of cancer/testis antigens (CTAs) on additional normal tissues or stem cells may restrict their use as cancer targets. The objective of the present study was to evaluate the mRNA levels of some CTAs in a variety of tissues.
  
  
  METHODS
  mRNA of pericytes, fibroblasts and mesenchymal stem cells (MSCs) derived from adult and fetal tissues, human umbilical vein endothelial cells, MSC-derived adipocytes, selected normal tissues and control cancer cell lines (CLs) were extracted and quantitative polymerase chain reaction was performed for MAGED1, PRAME, CTAG1B, MAGEA3 and MAGEA4.
  
  
  RESULTS
  MAGED1 was expressed in all normal tissues and cells evaluated. CTAG1B was expressed at levels comparable to control CLs on MSCs derived from arterial, fetal skin, adipose tissue and saphenous vein, heart, brain and skin tissues. MAGEA4 was detected only in fibroblasts and differentiated adipocytes from MSCs, at levels comparable to the control CLs.
  
  
  CONCLUSIONS
  The potential use of CTAs in immunotherapy should take into account the potential off-target effects on MSCs.
authors.affiliation: Fundação Hemocentro de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Saldanha-Araujo
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Haddad
authors.forename: Dalila Lucíola
authors.initials: DL
authors.lastname: Zanette
authors.forename: Amélia Goes
authors.initials: AG
authors.lastname: De Araujo
authors.forename: Maristela Delgado
authors.initials: MD
authors.lastname: Orellana
authors.forename: Dimas Tadeu
authors.initials: DT
authors.lastname: Covas
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Zago
authors.forename: Rodrigo Alexandre
authors.initials: RA
authors.lastname: Panepucci
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D058950:Melanoma-Specific Antigens; D008565:Membrane Proteins / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D020286:Pericytes / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; C095204:MAGEA4 protein, human; C120897:MAGED1 protein, human; D058950:Melanoma-Specific Antigens; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C404944:PRAME protein, human