title: Nucleic acids determination using the complex of eriochrome black T and silver nanoparticles in a resonance light scattering technique.
abstract: A novel method for the determination of nucleic acids by using silver nanoparticle (AgNPs)-eriochrome black T (EBT) as a resonance light scattering (RLS) probe has been developed. Under optimum conditions, there are linear relationships between the quenching extent of RLS intensity and the concentration of nucleic acids in the range of 4.0×10(-9)-4.0×10(-7), 4.0×10(-7)-1.6×10(-6) g mL(-1) for fish sperm DNA (fsDNA) and 4.0×10(-8)-2.0×10(-6) g mL(-1) for yeast RNA (yRNA). Their detection limits (S/N=3) are 2.0 ng mL(-1) and 21 ng mL(-1), respectively. The results indicate that AgNPs can form wirelike aggregates and nanoslices in the presence of the EBT. Whereas, when nucleic acids are added into the AgNPs-EBT system, the dynamic balance of AgNPs-EBT system is destroyed and the nanoparticles undergo dispersion again, leading to the RLS intensity of AgNPs-EBT system quenching. Meanwhile, the conformation of fsDNA is changed by the synergistic effect of AgNPs and EBT.
authors.affiliation: Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Shanda Nanlu 27#, Jinan 250100, PR China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Zhou
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Fanhui
authors.initials: F
authors.lastname: Meng
authors.forename: Jinghe
authors.initials: J
authors.lastname: Yang
authors.forename: Minqin
authors.initials: M
authors.lastname: Wang
mesh_terms: D000042:Absorption; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D002138:Calibration; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000648:ultrastructure; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D008027:Light; D057230:Limit of Detection; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009696:Nucleic Acids / Q000032:analysis*; D012542:Scattering, Radiation; D012834:Silver / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*; D013094:Spermatozoa
chemical_list: D001391:Azo Compounds; D009696:Nucleic Acids; D012834:Silver; D004247:DNA; C014527:Eriochrome Black T