title: Evaluation of high-performance liquid chromatography laser-induced fluorescence for serum protein profiling for early diagnosis of oral cancer.
abstract: The present work deals with the evaluation of a high-performance liquid chromatography laser-induced fluorescence (HPLC-LIF) technique developed in our laboratory for early detection of oral cancer from protein profiles of body fluids. The results show that protein profiles of serum samples from a given class of samples, say, normal, premalignant, or malignant, are statistically very close to each other, while profiles of members of any class are significantly different from other classes. The performance of the technique is evaluated by the use of sensitivity and specificity pairs, receiver operating characteristic (ROC) analysis, and Youden's Index. The technique uses protein profile differences in serum samples, registered by the HPLC-LIF technique. The study is carried out using serum samples from volunteers diagnosed as normal or premalignant clinically, and as malignant by histopathology. The specificities and sensitivities of the HPLC-LIF method at an ideal threshold (M-distance = 2) for normal, malignant, and premalignant classes are 100, 69.5, and 61.5%, and 86.5, 87.5, and 87.5% respectively.
authors.affiliation: Manipal University, Centre for Atomic and Molecular Physics, Manipal-576 104, India.
authors.forename: Ajeetkumar
authors.initials: A
authors.lastname: Patil
authors.forename: Vijendra
authors.initials: V
authors.lastname: Prabhu
authors.forename: K S
authors.initials: KS
authors.lastname: Choudhari
authors.forename: V K
authors.initials: VK
authors.lastname: Unnikrishnan
authors.forename: Sajan D
authors.initials: SD
authors.lastname: George
authors.forename: Ravikiran
authors.initials: R
authors.lastname: Ongole
authors.forename: Keerthilatha M
authors.initials: KM
authors.lastname: Pai
authors.forename: Jayarama K
authors.initials: JK
authors.lastname: Shetty
authors.forename: Sujatha
authors.initials: S
authors.lastname: Bhat
authors.forename: Vasudevan Bhaskaran
authors.initials: VB
authors.lastname: Kartha
authors.forename: Santhosh
authors.initials: S
authors.lastname: Chidangil
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D005260:Female; D006801:Humans; D007834:Lasers; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D014408:Biomarkers, Tumor