title: Study on the interactions between ginsenosides and lysozyme under acidic condition by ESI-MS and molecular docking.
abstract: In order to study the different effects of ginsenosides with similar structures, research on interactions between ginsenoside Rg1, Re and lysozyme was carried out by electrospray ionization mass spectrometry (ESI-MS) and molecular docking. The 1:1 and 2:1 noncovalent complexes of ginsenosides and lysozyme were observed in the mass spectra and the dissociation constants for them were directly calculated based on peak intensities of lysozyme and its noncovalent complexes with ginsenosides. The results showed that the 1:1 complex of ginsenoside Rg1 and lysozyme was more stable than that of ginsenoside Re and lysozyme. As the acidity increased, the stabilities of the 1:1 complexes of Rg1, Re and lysozyme both decreased. Interestingly, as the acidity increased, the stability of the 2:1 complex of Rg1 and lysozyme increased while that of Re decreased. From the result of molecular docking, ginsenosides interacted with the active sites of lysozyme. And the stability of the complexes could be affected by the conformation changes of lysozyme as acidity increased.
authors.affiliation: College of Grain Oil and Food Science, Henan University of Technology, 140 Songshan South Road, Zhengzhou 450052, China. quchenling@gmail.com
authors.forename: Chenling
authors.initials: C
authors.lastname: Qu
authors.forename: Songcheng
authors.initials: S
authors.lastname: Yu
authors.forename: Aixi
authors.initials: A
authors.lastname: Bai
authors.forename: Jinshui
authors.initials: J
authors.lastname: Wang
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D009113:Muramidase / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D036145:Ginsenosides; C049864:ginsenoside Re; D009113:Muramidase; C035054:ginsenoside Rg1; D019342:Acetic Acid