title: Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release.
abstract: In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain. carles.gil@uab.cat
authors.forename: Carles
authors.initials: C
authors.lastname: Gil
authors.forename: Anton
authors.initials: A
authors.lastname: Falqués
authors.forename: Eduard
authors.initials: E
authors.lastname: Sarró
authors.forename: Roger
authors.initials: R
authors.lastname: Cubi
authors.forename: Juan
authors.initials: J
authors.lastname: Blasi
authors.forename: José
authors.initials: J
authors.lastname: Aguilera
authors.forename: Emilio
authors.initials: E
authors.lastname: Itarte
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D047390:Casein Kinase II / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D050600:SNARE Proteins / Q000378:metabolism; D013574:Synaptosomes / Q000737:chemistry*; D050827:Syntaxin 1 / Q000378:metabolism
chemical_list: D018377:Neurotransmitter Agents; D047428:Protein Kinase Inhibitors; D050600:SNARE Proteins; D050827:Syntaxin 1; D047390:Casein Kinase II