title: Regulation of epidermal growth factor receptor through interaction of ganglioside GM3 with GlcNAc of N-linked glycan of the receptor: demonstration in ldlD cells.
abstract: We investigated interaction of GM3 with N-acetylglucosamine (GlcNAc) termini of N-linked glycans of epidermal growth factor receptor (EGFR), as the underlying mechanism for inhibitory effect of GM3 on EGFR activation, using ldlD cells transfected with EGFR gene. These cells, defective in UDP-Gal/UDP-GalNAc 4-epimerase, are incapable of synthesizing galactose (Gal)-containing glycans, unless Gal is provided in culture (+Gal). Key observations: (1) Expression of GlcNAc termini was high in -Gal cells, and strongly reduced in +Gal cells. (2) Comparative study of inhibitory effect of exogenously-added GM3 on EGFR activation in +Gal versus -Gal cells indicated that higher level of GlcNAc termini on EGFR is correlated with greater inhibitory effect of GM3. (3) GM3-, but not GM1-, coated beads bound to EGFR in lysate of -Gal cells, which have highly exposed GlcNAc termini. Such binding was inhibited in the presence of EDTA, similarly to other carbohydrate-carbohydrate interactions.
authors.affiliation: Division of Biomembrane Research, Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Guan
authors.forename: Kazuko
authors.initials: K
authors.lastname: Handa
authors.forename: Sen-Itiroh
authors.initials: SI
authors.lastname: Hakomori
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002465:Cell Movement / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D005679:G(M3) Ganglioside / Q000378:metabolism*; D010766:Phosphorylation; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005679:G(M3) Ganglioside; D011134:Polysaccharides; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D000117:Acetylglucosamine