title: [Glucocorticoids up-regulate human chorion 11beta-hydroxysteroid dehydrogenase type 1].
abstract: OBJECTIVE
  To study the co-localization of glucocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) and to investigate whether glucocorticoids regulate the reductase activity and expression of 11beta-HSD1.
  
  
  METHODS
  Immunohistochemical staining for 11beta-HSD1 in cultured primary human chorionic trophoblasts was performed. Radiometric conversion assay and Northern blot analysis were respectively used to observe the activity and mRNA expression of 11beta-HSD1.
  
  
  RESULTS
  11beta-HSD1 and GR were co-expressed in the same chorionic trophoblast. Both 11beta-HSD1 reductase activity and mRNA levels were increased by dexamethasone (10(-6) mol/L, 10(-7) mol/L) in the cultured chorionic trophoblasts, and the effects were blocked by GR antagonist RU486 (10(-6) mol/L).
  
  
  CONCLUSIONS
  By binding to GR, glucocorticoids induce the expression of 11beta-HSD1 by a possible intracrine mechanism.
authors.affiliation: Department of Physiology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.forename: Gang
authors.initials: G
authors.lastname: Sun
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000378:metabolism*; D002478:Cells, Cultured; D002823:Chorion / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D005938:Glucocorticoids; D012333:RNA, Messenger; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; C468386:HSD11B1 protein, human