title: [The study of the migration mechanism of murine flMSCs in ischemic injured brain].
abstract: OBJECTIVE
  To investigate the migration mechanism of murine flMSCs in ischemic injured brain.
  
  
  METHODS
  flMSCs were isolated and cultured in vitro. BALB/c mice were adopted to build model of cerebral ischemia. Chemokine receptor mRNA in flMSCs and SDF-1alpha (the only ligand for CXCR4) mRNA in injured brains were determined by RT-PCR. Expression and distribution of SDF-1alpha protein was detected by Western blot and immunohistochemistry. Migration of flMSCs induced by SDF-1alpha in vitro was detected by Boyden chamber assay.
  
  
  RESULTS
  Murine flMSCs expressed a restricted set of chemokine receptors including CR1, CR3, CXCR1, CXCR2, CXCR3, CXCR4. Expression of SDF-1alpha mRNA was significantly increased in ischemic injured brain compared with those in normal brain (P < 0.01). Protein level of SDF-1alpha in ischemic injured brain (12 h: 0.35 +/- 0.05, 24 h: 0.35 +/- 0.05, 48 h: 0.88 +/- 0.04) was statistically up-regulated compared with those in normal brains (0.74 +/- 0.07, P < 0.01). Expression of SDF-1alpha was significantly increased in peri-ischemia area such as cerebral cortex and hippocampus 24 h after ischemic injury. Expression of SDF-1alpha wasn't detected in contralateral hemisphere and normal brain. Migration of flMSCs could be induced by SDF-1alpha and blocked by CXCR4 antibody in vitro.
  
  
  CONCLUSIONS
  Interaction between CXCR4 and SDF-1alpha is one of the mechanisms in directing murine flMSCs migration in ischemic injured brain.
authors.affiliation: Institute of Hematology, Jinan University Medical College, Guangzhou 510632, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Dongmei
authors.initials: D
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology*; D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005333:Fetus; D008099:Liver / Q000166:cytology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control
chemical_list: C514936:CXCR4 protein, mouse; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D012333:RNA, Messenger; D019718:Receptors, CXCR4