title: [Changes of contents of extracellular signal-regulated kinase in rat hepatic fibrogenesis induced by bile duct ligation].
abstract: OBJECTIVE
  To observe the dynamic expression of ERK1 in fibrotic rat liver.
  
  
  METHODS
  The rat hepatic fibrosis was induced by bile duct ligation (BDL). Histopathological changes were evaluated by hematoxylin and eosin staining, and by Masson's trichrome method. ERK1 mRNA in liver was determined by reverse transcription-polymerase chain reaction (RT-PCR), while the distribution of ERK1 was assessed by immunohistochemistry. ERK1 protein was detected by using Western blotting analysis.
  
  
  RESULTS
  With the development of hepatic fibrosis, the positive cells of ERK1 increased a lot, they were mainly distributed at portal ducts, fiber septa and around the bile ducts, vascular endothelial cells and perisinusoidal cells. Western blotting analysis results displayed that the expression of ERK1 protein were up-regulated with model course, and its levels were the highest at week 4 after operation, achieving to 3.9-fold of that in normal rat liver. ERK1 mRNA expressed in normal rat livers as well, they were up-regulated at day 2 after BDL and its level was the highest at week 4 after BDL.
  
  
  CONCLUSIONS
  These data suggest that the expression of ERK1 and its mRNA can be increase greatly in fibrotic rat liver.
authors.affiliation: Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Zhang
authors.forename: Ze-ming
authors.initials: ZM
authors.lastname: Sun
authors.forename: Mei
authors.initials: M
authors.lastname: Song
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008105:Liver Cirrhosis, Biliary / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D048052:Mitogen-Activated Protein Kinase 3