title: Encapsulation of bacterial cells in electrospun microtubes.
abstract: Encapsulation of whole microbial cells in microtubes for use in bioremediation of pollutants in water systems was the main focus of this investigation. Coelectrospinning of a core polymeric solution with bacterial cells and a shell polymer solution using a spinneret with two coaxial capillaries resulted in microtubes with porous walls. The ability of the microtube's structure to support cell attachment and maintain enzymatic activity and proliferation of the encapsulated microbial cells was examined. The results obtained show that the encapsulated cells maintain some of their phosphatase, β-galactosidase and denirification activity and are able to respond to conditions that induce these activities. This study demonstrates electrospun microtubes are a suitable platform for the immobilization of intact microbial cells.
authors.affiliation: Faculty of Civil and Environmental Engineering, Technion, Israel Institute of Technology Haifa 32000, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Klein
authors.forename: J
authors.initials: J
authors.lastname: Kuhn
authors.forename: R
authors.initials: R
authors.lastname: Avrahami
authors.forename: S
authors.initials: S
authors.lastname: Tarre
authors.forename: M
authors.initials: M
authors.lastname: Beliavski
authors.forename: M
authors.initials: M
authors.lastname: Green
authors.forename: E
authors.initials: E
authors.lastname: Zussman
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000378:metabolism; D001422:Bacterial Adhesion; D001673:Biodegradation, Environmental; D002214:Capsules / Q000737:chemistry*; D018914:Cells, Immobilized / Q000378:metabolism*; D043942:Nanotubes / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*; D016062:Porosity; D014876:Water Pollution
chemical_list: D002214:Capsules; D011108:Polymers