title: Transcriptional activities of histone H3, cyclin D1 and claudin 7 encoding genes in laryngeal cancer.
abstract: Uncontrolled proliferation and a decrease in cell-cell adhesion are one of the most important characteristics of malignancy. Determination of replication-dependent histone H3 can be applied as a proliferative marker. Cyclin D1 (CCND1) regulates the cell cycle by participating in the control of the G1/S phase transition. Claudins (CLDN) are components of tight junctions and may play an essential role in the loss of tissue cohesion. The aim of the study was to assess the mRNA expression of histone H3, cyclin D1, and claudin 7 genes in laryngeal squamous cell carcinoma (LSCC) and adjacent nonneoplastic tissues. The study group consisted of 32 patients with LSCC. Adjacent nonneoplastic tissues of incision lines were used as controls. Quantification of H3, CCND1 and CLDN7 mRNAs was performed by the use of real-time QRT-PCR assay. Molecular analysis showed a significantly higher expression of CCND1 (P = 0.0001; Wilcoxon test) and H3 (P = 0.0141) genes in tumor tissues than in surrounding nonneoplastic tissues. On the contrary, transcriptional activity of claudin 7 gene was higher in histologically normal tissues; however, this difference was not statistically significant (P = 0.1499). The data obtained indicate that laryngeal cancer is characterized by high proliferative potential mediated by increase in cyclin D1 and H3 mRNAs expression.
authors.affiliation: Department of Biochemistry, Medical University of Silesia, Narcyzow 1, 41-200, Sosnowiec, Poland. mkapral@sum.edu.pl
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kapral
authors.forename: Barbara
authors.initials: B
authors.lastname: Strzalka-Mrozik
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kowalczyk
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Paluch
authors.forename: Joanna
authors.initials: J
authors.lastname: Gola
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gierek
authors.forename: Ludmila
authors.initials: L
authors.lastname: Weglarz
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D057167:Claudins; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019108:Tight Junctions / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: C473255:CLDN7 protein, human; D057167:Claudins; D006657:Histones; D008565:Membrane Proteins; D019938:Cyclin D1