title: [Effects of anoxia/reoxygenation on Fos and Jun expression and apoptosis in cultured rat hippocampal neurons].
abstract: OBJECTIVE
  To investigate the effects of anoxia/reoxygenation on Fos and Jun expression and apoptosis in cultured rat hippocampal neurons.
  
  
  METHODS
  The hippocampal neurons cultured for 12 d were exposed to anoxia environment (90% N2 + 10% CO2) for 4 h and then reoxygenated for 24 h and 72 h. The neurons were immunocytochemically stained using the antiserum against Fos and Jun, and the apoptosis were detected by using the terminal deoxynucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method and flow cytometric analysis.
  
  
  RESULTS
  The percentage of Fos and Jun positive neurons and apoptosis neurons in cultured hippocampal neurons after anoxia/reoxygenation increased than those in control.
  
  
  CONCLUSIONS
  The occurrence of neurons apoptosis is related to the increase in Fos and Jun expression in cultured hippocampal neurons after anoxia/reoxygenation.
authors.affiliation: Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ai-Shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Fu-Zhuang
authors.initials: FZ
authors.lastname: Wang
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016762:Genes, fos; D016758:Genes, jun; D006624:Hippocampus / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010100:Oxygen