title: [The effects of cell growth factors on proliferation and migration of coronary artery endothelial cells].
abstract: OBJECTIVE
  To study the effects of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on proliferation and migration of bovine coronary artery endothelial cells (BCAEC) in vitro.
  
  
  METHODS
  BCAECs were isolated and cultured in vitro, and divided into control group, VEGF group and HGF group. BCACEs proliferation were measured using MTT, and their migration was observed using reverse microscope.
  
  
  RESULTS
  The OD value of control, VEGF and HGF group were 0.22 +/- 0.01, 0.40 +/- 0.14, 0.44 +/- 0.15 respectively. The proliferation ratio of BCAECs in VEGF and HGF group was 81.8% +/- 16.9%, 100.0% +/- 21.1% respectively. There was no migration in control group, but significant migration in VEGF and HGF group.
  
  
  CONCLUSIONS
  Both VEGF and HGF can promote proliferation and migration of BCAECs, the effect of HGF is stronger than VEGF.
authors.affiliation: Department of Cardiology, Navy 411 Hospital, Shanghai 200081, China.
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Jiang
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Lin
authors.forename: Shuang-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Chuan-Xin
authors.initials: CX
authors.lastname: Lu
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000166:cytology; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D017077:Culture Media, Conditioned; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor