title: [Study on immunogenicity of DNA vaccine encoding human cytomegalovirus gB and pp150 fusion gene].
abstract: OBJECTIVE
  To provide experimental evidence for development of human cytomegalovirus (HCMV) nucleic acid vaccine, HCMV surface protein (gB), membrane protein (pp150), and gB-pp150 fused gene eukaryotic expression vector were constructed.
  
  
  METHODS
  gB and pp150 genes were amplified and fused into gB-pp150, then were cloned into pcDNA 3.1 (+) to obtain recombinant expression plasmids pcDNA 3.1 (+) -gB, pcDNA 3.1 (+) -pp150 and pcDNA 3.1 (+) -gB-pp150, which were encapsulated with chitosan. Mouse were vaccinated and the humoral and cell immune response were determined by ELISA, specific proliferative response of plenic lymphocytes.
  
  
  RESULTS
  The gB, pp150 and gB-pp150 fusion gene eukaryotic expression vector were successfully constructed. The antibodies A value induced by pcDNA3.1(+) -gB or pcDNA3.1 (+) -gB-pp150 were much higher than that of pcDNA3.1 (+) (P < 0.01). The IFN-gamma levels induced by pcDNA3.1 (+) -pp150 and pcDNA3.1 (+) -gB-pp150 were significantly higher than that of pcDNA3.1 (+). There are significant diference between the stimulating indexes of pcDNA3.1(+) -pp150 or pcDNA3.1 (+) -gB-pp150 immunized and normal mice.
  
  
  CONCLUSIONS
  The DNA vaccine pcDNA3.1 (+) -gB can induce significant humoral immunity response, and pcDNA3.1 (+) -pp150 can induce high cellular immune response, whereas pcDNA3.1 (+) -gB-pp150 can induce both humoral and cellar immune responses in BALB/c mice.
authors.affiliation: The First People's Hospital of Chenzhou, Chenzhou 423000, China. swychydd3@yahoo.com.cn
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Shi
authors.forename: Fu-Sheng
authors.initials: FS
authors.lastname: Yan
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zeng
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Ouyang
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Chen
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus