title: Dihydrotestosterone administration does not increase intraprostatic androgen concentrations or alter prostate androgen action in healthy men: a randomized-controlled trial.
abstract: BACKGROUND
  Concern exists that androgen treatment might adversely impact prostate health in older men. Dihydrotestosterone (DHT), derived from local conversion of testosterone to DHT by 5α-reductase enzymes, is the principal androgen within the prostate. Exogenous androgens raise serum DHT concentrations, but their effects on the prostate are not clear.
  
  
  OBJECTIVE
  To determine the impact of large increases in serum DHT concentrations on intraprostatic androgen concentrations and androgen action within the prostate.
  
  
  METHODS
  Double-blind, randomized, placebo-controlled.
  
  
  METHODS
  Single academic medical center.
  
  
  METHODS
  31 healthy men ages 35-55.
  
  
  METHODS
  Daily transdermal DHT or placebo gel.
  
  
  METHODS
  Serum and prostate tissue androgen concentrations and prostate epithelial cell gene expression after 4 wk of treatment.
  
  
  RESULTS
  Twenty-seven men completed all study procedures. Serum DHT levels increased nearly sevenfold, while testosterone levels decreased in men treated with daily transdermal DHT gel but were unchanged in the placebo-treated group (P < 0.01 between groups). In contrast, intraprostatic DHT and testosterone concentrations on d 28 were not different between groups (DHT: placebo = 2.8 ± 0.2 vs. DHT gel = 3.1 ± 0.5 ng/g; T: placebo = 0.6 ± 0.2 vs. DHT gel = 0.4 ± 0.1, mean ± se). Similarly, prostate volume, prostate-specific antigen, epithelial cell proliferation, and androgen-regulated gene expression were not different between groups.
  
  
  CONCLUSIONS
  Robust supraphysiologic increases in serum DHT, associated with decreased serum T, do not significantly alter intraprostatic levels of DHT, testosterone, or prostate epithelial cell androgen-regulated gene expression in healthy men. Changes in circulating androgen concentrations are not necessarily mimicked within the prostate microenvironment, a finding with implications for understanding the impact of androgen therapies in men.
authors.affiliation: Division of Metabolism, Endocrinology, and Nutrition, University of Washington School of Medicine, Box 357138, 1959 NE Pacific Street, Seattle, Washington 98195, USA. page@u.washington.edu
authors.forename: Stephanie T
authors.initials: ST
authors.lastname: Page
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Lin
authors.forename: Elahe A
authors.initials: EA
authors.lastname: Mostaghel
authors.forename: Brett T
authors.initials: BT
authors.lastname: Marck
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Wright
authors.forename: Jennifer
authors.initials: J
authors.lastname: Wu
authors.forename: John K
authors.initials: JK
authors.lastname: Amory
authors.forename: Peter S
authors.initials: PS
authors.lastname: Nelson
authors.forename: Alvin M
authors.initials: AM
authors.lastname: Matsumoto
mesh_terms: D000328:Adult; D000728:Androgens / Q000097:blood / Q000378:metabolism* / Q000502:physiology*; D049109:Cell Proliferation; D013196:Dihydrotestosterone / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D004311:Double-Blind Method; D023381:Endpoint Determination; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000032:analysis / Q000378:metabolism; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000378:metabolism
chemical_list: D000728:Androgens; D019394:Ki-67 Antigen; D013196:Dihydrotestosterone; D017430:Prostate-Specific Antigen