title: [Effects of CGRP on LPS-induced MMP-9 secretion by alveolar macrophages].
abstract: OBJECTIVE
  To explore the effects of calcitonin-gene-related peptide (CGRP) on LPS-induced MMP-9 secretion by alveolar macrophages (AM) in vitro.
  
  
  METHODS
  The supernatant of LPS-induced Wistar rat AM from different intervention groups were collected to measure the activity by gelatin zymography.
  
  
  RESULTS
  (Only secreting a small amount of MMP-9 with unstimulated AM, LPS stimulated MMP-9 production in a concentration-dependent manner (p < 0.01). (2) The activity of MMP-9 in CGRP intervention groups at different levels were significantly lower than those in non-intervention group (p < 0.01). (3) The inhibiting effects of CGRP were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (p < 0.05).
  
  
  CONCLUSIONS
  These data suggested that CGRP involved in the MMP-9 secretion by AM, partly, via PKC and CaM pathway.
authors.affiliation: Department of Physiology, Xiang Ya Medical School, Central South University, Changsha 410078, China.
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Cha-xiang
authors.initials: CX
authors.lastname: Guan
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Bai
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yan-ru
authors.initials: YR
authors.lastname: Cui
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Liu
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008070:Lipopolysaccharides / Q000009:adverse effects; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D018015:Receptors, Calcitonin Gene-Related Peptide; D020780:Matrix Metalloproteinase 9