title: Arachidonic acid induces production of 17,20β-dihydroxy-4-pregnen-3-one (DHP) via a putative PGE2 receptor in fish follicles from the Eurasian perch.
abstract: The effects of docosahexaenoic, eicosaenoic and arachidonic acids (DHA, EPA and ARA, respectively) on sex-steroid and prostaglandin (PG) production were investigated in Eurasian perch (Perca fluviatilis) follicles using an in- vitro incubation technique. Only ARA was able to induce the production of 17,20β-dihydroxy-4-pregnen-3-one (DHP), the hormone produced by vitellogenic follicles undergoing final meiotic maturation, as well as the production of PGE2 and PGF2α by the follicles. This work also investigated, using a preliminary pharmacological approach, the presence of a functional PGE2-like receptor in fish follicles. Exogenous PGE2 and butaprost (specific agonist of the EP2 receptor) stimulated DHP production. A second experiment assayed the cyclic adenosine monophosphate (cAMP) production by the follicles after 24 h of incubation with the agonist and antagonist of the EP2 receptor. As observed in mammals, we concluded that the cAMP produced in response to PGE2 was probably mediated by an intracellular mechanism via a PGE2-like receptor. This is the first pharmacological indication of this type of receptors in fish follicles. This study also indicates that ARA, and its derivatives, PGE2 and PGF2α, may act on final follicle maturation in Eurasian perch.
authors.affiliation: Unit of Research in Organismal Biology, University of Namur, 61 Rue de Bruxelles, 5000 Namur, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Henrotte
authors.forename: S
authors.initials: S
authors.lastname: Milla
authors.forename: S N M
authors.initials: SN
authors.lastname: Mandiki
authors.forename: P
authors.initials: P
authors.lastname: Kestemont
mesh_terms: D000527:Alprostadil / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D000242:Cyclic AMP; D015232:Dinoprostone / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005260:Female; D006908:Hydroxyprogesterones / Q000378:metabolism*; D008540:Meiosis; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D010471:Perches / Q000378:metabolism / Q000502:physiology*; D018078:Receptors, Prostaglandin E / Q000378:metabolism*; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D006908:Hydroxyprogesterones; D018078:Receptors, Prostaglandin E; D058307:Receptors, Prostaglandin E, EP2 Subtype; C007433:17,20-dihydroxy-4-pregnen-3-one; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid; D000242:Cyclic AMP; D000527:Alprostadil; C048491:butaprost; D015232:Dinoprostone