title: A potential inhibitor of rat aortic vascular smooth muscle cell proliferation from the pollen of Typha angustata.
abstract: By various chromatographic methods, three flavonoids, (2S)-naringenin (1), isorhamnetin 3-O-(2-O-α-L-rhamnopyranosyl) β-D-glucopyranoside (2), typhaneoside (3), and two sterol glycosides, β-sitosterol-3-O-(6-octadecanoyl) β-D-glucopyranoside (4) and β-sitosterol-3-O-(6-octadeca-9Z,12Z-dienoyl) β-D-glucopyranoside (5), were isolated from the pollen of Typha angustata. Their structures were determined on the basis of spectroscopic analyses. The flavonoids (1-3) were evaluated for their effects on the viability and proliferation of rat aortic smooth muscle cells. (2S)-naringenin (1) significantly inhibited cell proliferation in a dose-dependent manner without cytotoxic at concentrations of 30, and 50 μM; it reduced the number of cells following PDGF-BB treatment to 1.83 ± 0.30 × 10(4) and 2.20 ± 0.60 × 10(4) cells/well, respectively. These findings suggest that (2S)-naringenin has antiproliferative effects on aortic smooth muscle cells.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Nhiem
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Jung-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Ja Hyeong
authors.initials: JH
authors.lastname: Ku
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Myung
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects; D000077214:Becaplermin; D049109:Cell Proliferation / Q000187:drug effects*; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011058:Pollen; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D032401:Typhaceae
chemical_list: D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin