title: Chemo-physical and biological evaluation of poly(L-lysine)-grafted chitosan copolymers used for highly efficient gene delivery.
abstract: For the success of non-viral gene delivery, it is of great importance to develop gene vectors with high efficiency but low toxicity. We demonstrate that PLL-grafted chitosan copolymers combine the advantages of PLL with its good pDNA-binding ability and of chitosan with its good biocompatibility. The chemo-physical properties of the prepared Chi-g-PLL copolymers are thoroughly characterized. The in vitro transfection study shows that the copolymers have a much higher gene transfer ability than the starting materials chitosan and PLL. A positive correlation between PLL chain lengths and transfection efficiency of the copolymers is found. Our results suggest that these novel Chi-g-PLL copolymers are good candidates for gene delivery in vivo.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Yu
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.forename: Huayu
authors.initials: H
authors.lastname: Tian
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Lu
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
mesh_terms: D002470:Cell Survival; D055598:Chemical Phenomena; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011107:Polylysine / Q000138:chemical synthesis / Q000737:chemistry*; D055672:Static Electricity; D014162:Transfection
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011107:Polylysine; D004247:DNA; D048271:Chitosan