title: RiceFOX: a database of Arabidopsis mutant lines overexpressing rice full-length cDNA that contains a wide range of trait information to facilitate analysis of gene function.
abstract: Identification of gene function is important not only for basic research but also for applied science, especially with regard to improvements in crop production. For rapid and efficient elucidation of useful traits, we developed a system named FOX hunting (Full-length cDNA Over-eXpressor gene hunting) using full-length cDNAs (fl-cDNAs). A heterologous expression approach provides a solution for the high-throughput characterization of gene functions in agricultural plant species. Since fl-cDNAs contain all the information of functional mRNAs and proteins, we introduced rice fl-cDNAs into Arabidopsis plants for systematic gain-of-function mutation. We generated >30,000 independent Arabidopsis transgenic lines expressing rice fl-cDNAs (rice FOX Arabidopsis mutant lines). These rice FOX Arabidopsis lines were screened systematically for various criteria such as morphology, photosynthesis, UV resistance, element composition, plant hormone profile, metabolite profile/fingerprinting, bacterial resistance, and heat and salt tolerance. The information obtained from these screenings was compiled into a database named 'RiceFOX'. This database contains around 18,000 records of rice FOX Arabidopsis lines and allows users to search against all the observed results, ranging from morphological to invisible traits. The number of searchable items is approximately 100; moreover, the rice FOX Arabidopsis lines can be searched by rice and Arabidopsis gene/protein identifiers, sequence similarity to the introduced rice fl-cDNA and traits. The RiceFOX database is available at http://ricefox.psc.riken.jp/.
authors.affiliation: RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan. stetsuya@psc.riken.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kondou
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurotani
authors.forename: Mieko
authors.initials: M
authors.lastname: Higuchi
authors.forename: Takanari
authors.initials: T
authors.lastname: Ichikawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kuroda
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Saitou
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Shoji
authors.initials: S
authors.lastname: Sugano
authors.forename: Makoto
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Shinya
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takatsuji
authors.forename: Naoki
authors.initials: N
authors.lastname: Yokotani
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshizumi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Oda
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hirochika
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D030541:Databases, Genetic; D018745:Genome, Plant; D020407:Internet; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014584:User-Computer Interface
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant