title: [The experimental study on protective effects and its mechanisms of interleukin-1 receptor antagonist on isolated swine kidney with ischemia/reperfusion].
abstract: OBJECTIVE
  To observe the protective effects and approach its mechanisms of interleukin-1 receptor antagonist (IL-1ra) on the isolated swine kidney with ischemia/reperfusion (IR).
  
  
  METHODS
  Thirty two swine were divided randomly into control group (n = 11), IR group (n = 11) and IL-1ra treatment group (IL-1ra group, n = 10) in the study. All swine were electrified. The kidneys were removed and put in ice-box immediately for detecting in control group. In IR group and IL-1ra group, reperfusion pressure and flow rate were both observed once every 30 minutes, the urine at the time of reperfusion 2 hours and the renal cortex at the time of reperfusion 2 hours and 30 minutes were taken for detecting.
  
  
  RESULTS
  The resistance of perfusion in IL-1ra group was much lower than that in the IR group during reperfusion except start of reperfusion, there was a significant difference between IR group and IL-1ra group (P < 0.05, under the same). The content of MDA,TNF-alpha and IL-8 in the renal cortex in IR group were higher and the activities of SOD and Na(+) -K(+) -ATPase were lower than those in control group, In IL-1ra group, the content of MDA and the activity of SOD in renal cortex was respectively higher or lower than that in control group. Compared with IR group, the content of MDA and TNF-alpha and IL-8 in the renal tissues and urine protein were lower and the activities of SOD and Na(+) -K(+) -ATPase were higher in IL-1ra group. The serious degeneration and necrosis of renal tissue were observed in IR group under light microscope and electron microscope, but there was only slight degeneration in IL-1ra group.
  
  
  CONCLUSIONS
  The results indicated that IL-1ra were effective in protecting the isolated swine kidney from IR injury.
authors.affiliation: Department of Physiology, Luzhou Medical College, Luzhou 646000, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Zhao
authors.forename: C L
authors.initials: CL
authors.lastname: Zhang
authors.forename: L H
authors.initials: LH
authors.lastname: Li
authors.forename: Z C
authors.initials: ZC
authors.lastname: Feng
authors.forename: X H
authors.initials: XH
authors.lastname: Li
authors.forename: D B
authors.initials: DB
authors.lastname: Li
mesh_terms: D000818:Animals; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism / Q000517:prevention & control*; D007668:Kidney / Q000098:blood supply*; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013552:Swine
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1