title: Evaluation of Fast-ELISA versus standard-ELISA to diagnose human fasciolosis.
abstract: BACKGROUND
  The present study was conducted to evaluate and compare a fast-ELISA (F-ELISA) method versus standard-ELISA (S-ELISA) to diagnose human fasciolosis.
  
  
  METHODS
  Serum samples were obtained from 35 individuals infected with fasciolosis, 27 infected with other parasitoses and 22 from healthy people. The samples were examined with S-ELISA (30-minute incubation periods) and F-ELISA (10-minute incubation periods) for total antibody response against fasciolosis.
  
  
  RESULTS
  The optimum conditions for S-ELISA and F-ELISA were respectively as follows: antigen 10 and 5 µg/mL, sera 1:500 dilution for both, peroxidase-conjugated goat anti-human IgG diluted 1:7000 and 1:10000. Data were analyzed using SPSS version 11.0. Cut-off vale for S-ELISA and F-ELISA was determined as 0.56 and 0.42, respectively. The sensitivity, specificity; positive, and negative predictive values were detected as 97.2%, 100%, 94.6%, and 95.6% for both tests.
  
  
  CONCLUSIONS
  Cut-off values, sensitivity, specificity, and other important parameters of the two evaluated tests determined that the F-ELISA method could be used with no detectable difference.
authors.affiliation: Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Rahimi
authors.forename: Kayhan
authors.initials: K
authors.lastname: Ashrafi
authors.forename: Soheyla
authors.initials: S
authors.lastname: Koosha
authors.forename: Jahangir
authors.initials: J
authors.lastname: Abdi
authors.forename: Mohammad Bagher
authors.initials: MB
authors.lastname: Rokni
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth