title: One step at a time: action mechanism of Sushi 1 antimicrobial peptide and derived molecules.
abstract: Antimicrobial peptides (AMPs) are a crucial part of the innate immune system of eukaryotes and present a possible alternative to common antibiotics. It is therefore of great importance to understand their modes of action. Using a single-molecule approach in combination with high resolution imaging and bio-functional assays we were able to determine the different steps occurring during the action of the α-helical AMP Sushi 1 during bacterial lysis in spatial and temporal resolution in a biologically relevant context. Furthermore, we comment on the use of Sushi 1 as a template for new peptides to learn more about structure-function relationship of AMPs.
authors.affiliation: Singapore-MIT Alliance, Singapore.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Leptihn
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Vladimir
authors.initials: V
authors.lastname: Frecer
authors.forename: Bow
authors.initials: B
authors.lastname: Ho
authors.forename: Jeak Ling
authors.initials: JL
authors.lastname: Ding
authors.forename: Thorsten
authors.initials: T
authors.lastname: Wohland
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects*; D006737:Horseshoe Crabs / Q000737:chemistry* / Q000276:immunology*
chemical_list: D023181:Antimicrobial Cationic Peptides