title: Insamhodo-tang, a traditional Korean medicine, regulates mast cell-mediated allergic inflammation in vivo and in vitro.
abstract: OBJECTIVE
  Insamhodo-tang (IHT) has traditionally been used in Korea to treat a variety of diseases, including chronic cough, tuberculosis, and chronic bronchitis. However, the anti-allergic and anti-inflammatory effects of IHT and its molecular mechanisms have yet to be clearly elucidated. In this study, we attempted to evaluate the effects of IHT on mast cell-mediated allergy inflammation in vitro and in vivo.
  
  
  METHODS
  We investigated to ascertain the pharmacological effects of IHT on both compound 48/80-induced and 2,4-dinitrofluorobenzene (DNFB)-induced allergic reactions under in vivo conditions. Additionally, to find a possible explanation for the anti-inflammatory mechanisms of IHT, we evaluated the regulatory effects of IHT on the level of inflammatory mediators in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1).
  
  
  RESULTS
  The finding of this study demonstrated that IHT reduced compound 48/80-induced systemic anaphylactic shock, DNFB-induced dermatitis, and ear swelling responses in mice. Additionally, IHT inhibited the production of interleukin (IL)-6, IL-8, and TNF-α, as well as the activation of nuclear factor-κB and caspase-1 in PMACI-stimulated HMC-1.
  
  
  CONCLUSIONS
  Collectively, the findings of this study provide us with a novel insight into the pharmacological actions of IHT as a potential molecule for use in the treatment of allergic inflammation diseases.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701 Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Kee
authors.forename: In-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kim
authors.forename: Dae-Seung
authors.initials: DS
authors.lastname: Kim
authors.forename: Yong-Deok
authors.initials: YD
authors.lastname: Jeon
authors.forename: Se-Gun
authors.initials: SG
authors.lastname: Kim
authors.forename: Beom-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Jung
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D000707:Anaphylaxis / Q000188:drug therapy / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000001:Calcimycin; D003872:Dermatitis / Q000188:drug therapy / Q000378:metabolism; D004139:Dinitrofluorobenzene; D004487:Edema / Q000188:drug therapy; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D007476:Ionophores; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000473:pathology; D055670:Medicine, Korean Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D007476:Ionophores; D010936:Plant Extracts; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D004139:Dinitrofluorobenzene