title: Evaluation of serum bactericidal antibody assays for Haemophilus influenzae serotype a.
abstract: Haemophilus influenzae type a (Hia) is an important pathogen for some American Indian, Alaskan native, and Northern Canada aboriginal populations. Assays to measure serum bactericidal activity (SBA) to Hia have not been developed or validated. Here, we describe two methods for the measurement of SBA: SBA with a viability endpoint (CFU counts) and SBA with a fluorometric endpoint using alamarBlue as the metabolic indicator. Both SBA assays measure Hia-specific functional antibody and correlate with anti-Hia IgG enzyme-linked immunosorbent assay (ELISA) concentration of naturally acquired antibodies.
authors.affiliation: Division of Infectious Diseases, Emory University School of Medicine, 69 Jesse Hill Jr. Drive, Atlanta, Georgia 30303, USA.
authors.forename: Nadine G
authors.initials: NG
authors.lastname: Rouphael
authors.forename: Sarah
authors.initials: S
authors.lastname: Satola
authors.forename: Monica M
authors.initials: MM
authors.lastname: Farley
authors.forename: Karen
authors.initials: K
authors.lastname: Rudolph
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Schmidt
authors.forename: Patricia
authors.initials: P
authors.lastname: Gomez-de-León
authors.forename: John B
authors.initials: JB
authors.lastname: Robbins
authors.forename: Rachel
authors.initials: R
authors.lastname: Schneerson
authors.forename: George M
authors.initials: GM
authors.lastname: Carlone
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Steiner
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods*; D002170:Canada; D015169:Colony Count, Microbial; D005470:Fluorometry / Q000379:methods; D006192:Haemophilus Infections / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D050296:Microbial Viability; D008875:Middle Aged; D010078:Oxazines / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D058248:Serum Bactericidal Antibody Assay / Q000379:methods*; D014966:Xanthenes / Q000378:metabolism
chemical_list: D010078:Oxazines; D014966:Xanthenes; C005843:resazurin