title: Sequence analysis of the replicase gene of 'sweet potato caulimo-like virus' suggests that this virus is a distinct member of the genus Cavemovirus.
abstract: Virion purification from indicator plants and partial sequencing of the replicase region of a 'sweet potato caulimo-like virus' (SPCV) isolate from Madeira, Portugal, are described. Phylogenetic analysis suggests that SPCV is a distinct member of the genus Cavemovirus (family Caulimoviridae). These results explain previous failed attempts to characterize SPCV based on antibodies or primers designed for other members of the Caulimoviridae. Using a quick DNA extraction protocol and PCR primers flanking the RT motif region, we were able to detect SPCV directly in sweet potato, thus saving considerable time during routine virus indexing.
authors.affiliation: Virology Laboratory, Integrated Crop Management Division, International Potato Center (CIP), Lima, Peru.
authors.forename: Joao
authors.initials: J
authors.lastname: De Souza
authors.forename: Wilmer J
authors.initials: WJ
authors.lastname: Cuellar
mesh_terms: D052639:Caulimoviridae / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D027723:Ipomoea batatas / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011174:Portugal; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase