title: Characterization of ORF29 of Bombyx mori nucleopolyhedrovirus.
abstract: ORF 29 of Bombyx mori nucleopolyhedrovirus (BmNPV ORF29), a conserved gene among all the lepidopteran baculoviruses, was subjected to analysis of sequence and transcription/translation in the insect BmN cells in vitro. His-tagged BmNPV ORF29 fusion protein was expressed in Escherichia coli, purified and used for the production of an antiserum. All the homologs of BmNPV ORF29 in baculoviruses contain a Nudix motif with slight modifications in the N-terminal part. A 654 nts-long transcript of BmNPV ORF29 in BmN cells was detected at 3-48 hrs p.i. indicating the early nature of the gene. In the virus-infected cells, the BmNPV ORF29 protein of 26 K was present in both the cytoplasm and nucleus. In the virions, the protein was present in Budded virus (BV), but not in Occlusion-derived virus (ODV). These results suggest that BmNPV ORF29 is a functional ORF of BmNPV, which encodes a 26 K protein expressed in the early stage of infection cycle.
authors.affiliation: Jiangsu University, Zhenjiang, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: G
authors.initials: G
authors.lastname: Huang
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Yao
authors.forename: Z
authors.initials: Z
authors.lastname: Guo
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D021381:Protein Transport; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins