title: Immunotolerance reaction for allograft-limb in rats induced by gene-modified cell transfusion.
abstract: The therapeutic value of the transforming growth factor beta 1 (TGF-b) in transplantation has been reported; However, cell-mediated gene therapy using TGF-b is not applied to the organ transplantation widely. This study was to evaluate whether TGF-b-modified donor spleen cell specific transfusion in rat heterotopic allo-limb transplantation could induce tolerance tolerogenicity and prolong allograft's survival time. The Splenic T-cell in Wistar rats responsing to donor spleen cells which received TGF-b-transduced were severely impaired.The Survival time of Sprague-Dawley Allograft-limb in Wistar rats given TGF-b-modified donor spleen cells (5¥106 cells/well, administration of donor TGF-b-transduced donor spleen cells 7 days before transplantation) was extended modestly but significantly.
authors.affiliation: Department of Orthopedic Surgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: W J
authors.initials: WJ
authors.lastname: Yue
authors.forename: Q
authors.initials: Q
authors.lastname: Yue
authors.forename: D W
authors.initials: DW
authors.lastname: Yang
authors.forename: Q G
authors.initials: QG
authors.lastname: Meng
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002478:Cells, Cultured; D005240:Feasibility Studies; D015316:Genetic Therapy / Q000379:methods*; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D006085:Graft Survival; D006614:Hindlimb / Q000098:blood supply / Q000637:transplantation*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation*; D013997:Time Factors; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D023001:Transplantation Tolerance; D014184:Transplantation, Homologous
chemical_list: D016212:Transforming Growth Factor beta