title: Differential genomic targeting of the transcription factor TAL1 in alternate haematopoietic lineages.
abstract: TAL1/SCL is a master regulator of haematopoiesis whose expression promotes opposite outcomes depending on the cell type: differentiation in the erythroid lineage or oncogenesis in the T-cell lineage. Here, we used a combination of ChIP sequencing and gene expression profiling to compare the function of TAL1 in normal erythroid and leukaemic T cells. Analysis of the genome-wide binding properties of TAL1 in these two haematopoietic lineages revealed new insight into the mechanism by which transcription factors select their binding sites in alternate lineages. Our study shows limited overlap in the TAL1-binding profile between the two cell types with an unexpected preference for ETS and RUNX motifs adjacent to E-boxes in the T-cell lineage. Furthermore, we show that TAL1 interacts with RUNX1 and ETS1, and that these transcription factors are critically required for TAL1 binding to genes that modulate T-cell differentiation. Thus, our findings highlight a critical role of the cellular environment in modulating transcription factor binding, and provide insight into the mechanism by which TAL1 inhibits differentiation leading to oncogenesis in the T-cell lineage.
authors.affiliation: The Sprott Center for Stem Cell Research, Department of Regenerative Medicine, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada.
authors.forename: Carmen G
authors.initials: CG
authors.lastname: Palii
authors.forename: Carolina
authors.initials: C
authors.lastname: Perez-Iratxeta
authors.forename: Zizhen
authors.initials: Z
authors.lastname: Yao
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Fengtao
authors.initials: F
authors.lastname: Dai
authors.forename: Jerry
authors.initials: J
authors.lastname: Davison
authors.forename: Harold
authors.initials: H
authors.lastname: Atkins
authors.forename: David
authors.initials: D
authors.lastname: Allan
authors.forename: F Jeffrey
authors.initials: FJ
authors.lastname: Dilworth
authors.forename: Robert
authors.initials: R
authors.lastname: Gentleman
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Tapscott
authors.forename: Marjorie
authors.initials: M
authors.lastname: Brand
mesh_terms: D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006410:Hematopoiesis / Q000235:genetics* / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D050784:Proto-Oncogene Protein c-ets-1 / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D050676:Core Binding Factor Alpha 2 Subunit; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; C494041:ETS1 protein, human; C493728:RUNX1 protein, human; C067143:TAL1 protein, human