title: [Suppression to ameloblastoma xenografts of chicken embryo chorioallantoic membrane by tissue inhibitor of metalloproteinases-2].
abstract: OBJECTIVE
  To explore the invasiveness of xenografts on chicken embryo chorioallantoic membrane (CAM) after tissue inhibitor of metalloproteinase-2 (TIMP-2) gene transfection.
  
  
  METHODS
  Fresh ameloblastoma tissues were minced into 1-2 mm3 and transplanted on the CAM. There were three groups named as control group (Empt), plasma transfection group (Lipo), and TIMP-2 gene transfection group (P). The specimens were respectively investigated by microscope indifferent spots after implanting. The volume of the xenografts and the weight of xenografts in the termination time of the experiment were recorded. The invasiveness of xenografts was divided into four grades by pathological examination. Western blot analysis was performed to investigate matrix metalloproteinase-2 (MIMP-2) and TIMP-2 protein in xenografts.
  
  
  RESULTS
  Ameloblastoma tissues can survive on CAM and the tumor cells may invade it on 5-7 days after implanting. At 9 d after implanting, the invasiveness grades in P group were 7 in grade 0, 1 in grade 2, 0 in grade 3. The expression of TIMP-2 protein in P group was significantly higher than that in Empt group (P < 0.05). The expression of MMP-2 protein in P group was lower than that in Empt group (P < 0.05).
  
  
  CONCLUSIONS
  The xenotransplanted tumor model of human ameloblastoma on CAM was successfully established. The invasiveness of ameloblastoma xenografts was suppressed might be due to TIMP-2 gene transfection.
authors.affiliation: Dept. of Stomatology, Nanfang Hospital of Southern Medical University, Guangzhou 510515, China.
authors.forename: Lei-tao
authors.initials: LT
authors.lastname: Zhang
authors.forename: Wei-zhong
authors.initials: WZ
authors.lastname: Li
authors.forename: Hong-zhang
authors.initials: HZ
authors.lastname: Huang
authors.forename: Dong-lin
authors.initials: DL
authors.lastname: Zeng
authors.forename: Qian
authors.initials: Q
authors.lastname: Tao
mesh_terms: D000564:Ameloblastoma; D000818:Animals; D002645:Chickens; D049033:Chorioallantoic Membrane; D064593:Heterografts; D006801:Humans; D020778:Matrix Metalloproteinase 2; D019716:Tissue Inhibitor of Metalloproteinase-2; D014162:Transfection
chemical_list: C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2