title: Quantification of in vivo fluorescence decoupled from the effects of tissue optical properties using fiber-optic spectroscopy measurements.
abstract: We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast.
authors.affiliation: University of Toronto, Department of Medical Biophysics, Toronto, Ontario, Canada.
authors.forename: Anthony
authors.initials: A
authors.lastname: Kim
authors.forename: Mamta
authors.initials: M
authors.lastname: Khurana
authors.forename: Yumi
authors.initials: Y
authors.lastname: Moriyama
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
mesh_terms: D000465:Algorithms; D014408:Biomarkers, Tumor / Q000032:analysis*; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D003198:Computer Simulation; D003936:Diagnosis, Computer-Assisted / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D006801:Humans; D008954:Models, Biological; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D014408:Biomarkers, Tumor