title: Temporally and biochemically distinct activities of Exo1 during meiosis: double-strand break resection and resolution of double Holliday junctions.
abstract: The Rad2/XPG family nuclease, Exo1, functions in a variety of DNA repair pathways. During meiosis, Exo1 promotes crossover recombination and thereby facilitates chromosome segregation at the first division. Meiotic recombination is initiated by programmed DNA double-strand breaks (DSBs). Nucleolytic resection of DSBs generates long 3' single-strand tails that undergo strand exchange with a homologous chromosome to form joint molecule (JM) intermediates. We show that meiotic DSB resection is dramatically reduced in exo1Δ mutants and test the idea that Exo1-catalyzed resection promotes crossing over by facilitating formation of crossover-specific JMs called double Holliday junctions (dHJs). Contrary to this idea, dHJs form at wild-type levels in exo1Δ mutants, implying that Exo1 has a second function that promotes resolution of dHJs into crossovers. Surprisingly, the dHJ resolution function of Exo1 is independent of its nuclease activities but requires interaction with the putative endonuclease complex, Mlh1-Mlh3. Thus, the DSB resection and procrossover functions of Exo1 during meiosis involve temporally and biochemically distinct activities.
authors.affiliation: Howard Hughes Medical Institute, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Kseniya
authors.initials: K
authors.lastname: Zakharyevich
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Ma
authors.forename: Shangming
authors.initials: S
authors.lastname: Tang
authors.forename: Patty Yi-Hwa
authors.initials: PY
authors.lastname: Hwang
authors.forename: Serge
authors.initials: S
authors.lastname: Boiteux
authors.forename: Neil
authors.initials: N
authors.lastname: Hunter
mesh_terms: D055162:Biocatalysis; D003434:Crossing Over, Genetic; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*
chemical_list: D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I