title: Epigallocatechin gallate-loaded polysaccharide nanoparticles for prostate cancer chemoprevention.
abstract: OBJECTIVE
  Polysaccharide nanoparticles were studied as drug delivery vehicles for chemopreventive agents.
  
  
  METHODS
  Green tea polyphenol epigallocatechin-3-gallate (EGCG) was incorporated into a carbohydrate matrix of gum arabic and maltodextrin with an encapsulation efficiency of approximately 85%.
  
  
  RESULTS
  Encapsulated EGCG retained its biological activity, reducing the cell viability and inducing apoptosis of Du145 prostate cancer cells. Clonogenic assay demonstrated that encapsulation of EGCG enhanced its inhibitory effect on cell proliferation (10-20%) at lower concentrations (1-2 µM), compared with free EGCG.
  
  
  CONCLUSIONS
  This study highlights the use of polysaccharide nanoparticles in chemoprevention as they can be used to deliver natural antioxidants capable of inhibiting steps of the tumorigenesis process.
authors.affiliation: Laboratory for Process, Environmental & Energy Engineering, Chemical Engineering Department, Faculty of Engineering, University of Porto, Rua Roberto Frias, 4200-465 Porto, Portugal. sandra.rocha@fe.up.pt
authors.forename: Sandra
authors.initials: S
authors.lastname: Rocha
authors.forename: Roman
authors.initials: R
authors.lastname: Generalov
authors.forename: Maria do Carmo
authors.initials: Mdo C
authors.lastname: Pereira
authors.forename: Ivone
authors.initials: I
authors.lastname: Peres
authors.forename: Petras
authors.initials: P
authors.lastname: Juzenas
authors.forename: Manuel A N
authors.initials: MA
authors.lastname: Coelho
mesh_terms: D016588:Anticarcinogenic Agents / Q000008:administration & dosage* / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma / Q000517:prevention & control*; D053148:Caspase 3 / Q000378:metabolism; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018890:Chemoprevention; D006170:Gum Arabic / Q000737:chemistry; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000517:prevention & control*; D013662:Tea / Q000737:chemistry
chemical_list: D016588:Anticarcinogenic Agents; D011134:Polysaccharides; D013662:Tea; C008315:maltodextrin; D002392:Catechin; D006170:Gum Arabic; C045651:epigallocatechin gallate; D053148:Caspase 3