title: Postischemic administration of liposome-encapsulated luteolin prevents against ischemia-reperfusion injury in a rat middle cerebral artery occlusion model.
abstract: Oxidative stress-induced neuronal cell death has been implicated in neurodegenerative diseases; one such disease is ischemic stroke. Using reactive oxygen species (ROS)-insulted primary neurons, we screened neuroprotectants with clinical potential and then, using ischemia/reperfusion (I/R) model, investigated the anti-ischemic potential of candidate neuroprotectants. Here, we showed that luteolin, isolated from the ripe fruit of Perilla frutescens (L.) Britt, exhibited a neuroprotective action upon the in vitro platform, thus serving as candidate for in vivo pharmacological evaluation. Liposome-encapsulated luteolin produced dramatic preventing effects on I/R-induced behavioral and histological injuries after a 13-day post-ischemic treatment. Furthermore, this phytochemical not only lowered the increased level of mitochondrial ROS but also substantially up-regulated the decreased activity of catalase and glutathione in I/R rat brains. Collectively, luteolin as a neuroprotectant acts by anti-ischemic activity likely through a rebalancing of pro-oxidant/antioxidant status. Its multitarget mechanisms implicate potential effectiveness for clinically treating ischemia stroke.
authors.affiliation: Cell Star Bio-Technologies Co., Limited, Shanghai, People's Republic of China. zhaogangtcm@hotmail.com
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Shao-Yun
authors.initials: SY
authors.lastname: Zang
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Yao-Yue
authors.initials: YY
authors.lastname: Chen
authors.forename: Xun-He
authors.initials: XH
authors.lastname: Ji
authors.forename: Bu-Feng
authors.initials: BF
authors.lastname: Lu
authors.forename: Jia-Hu
authors.initials: JH
authors.lastname: Wu
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Qin
authors.forename: Li-He
authors.initials: LH
authors.lastname: Guo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D016923:Cell Death; D005260:Female; D005978:Glutathione / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000188:drug therapy*; D008081:Liposomes; D047311:Luteolin / Q000008:administration & dosage / Q000627:therapeutic use*; D008928:Mitochondria / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*
chemical_list: D000975:Antioxidants; D008081:Liposomes; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D002374:Catalase; D005978:Glutathione; D047311:Luteolin