title: SUMOylation of RIG-I positively regulates the type I interferon signaling.
abstract: Retinoic acid-inducible gene-I (RIG-I) functions as an intracellular pattern recognition receptor (PRR) that recognizes the 5'-triphosphate moiety of single-stranded RNA viruses to initiate the innate immune response. Previous studies have shown that Lys63-linked ubiquitylation is required for RIG-I activation and the downstream anti-viral type I interferon (IFN-I) induction. Herein we reported that, RIG-I was also modified by small ubiquitin-like modifier-1 (SUMO-1). Functional analysis showed that RIG-I SUMOylation enhanced IFN-I production through increased ubiquitylation and the interaction with its downstream adaptor molecule Cardif. Our results therefore suggested that SUMOylation might serve as an additional regulatory tier for RIG-I activation and IFN-I signaling.
authors.affiliation: Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Mi
authors.forename: Jihuan
authors.initials: J
authors.lastname: Fu
authors.forename: Yanbao
authors.initials: Y
authors.lastname: Xiong
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology; D001483:Base Sequence; D001665:Binding Sites; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate; D007370:Interferon Type I / Q000276:immunology / Q000502:physiology*; D034622:RNA Interference; D011971:Receptors, Immunologic; D025842:SUMO-1 Protein / Q000502:physiology; D029082:Sendai virus / Q000276:immunology; D015398:Signal Transduction; D058207:Sumoylation; D044763:Ubiquitin-Conjugating Enzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers; D007370:Interferon Type I; C505371:MAVS protein, human; D011971:Receptors, Immunologic; D025842:SUMO-1 Protein; C495501:SUMO1 protein, human; D044763:Ubiquitin-Conjugating Enzymes; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D000098843:Ubiquitin-Conjugating Enzyme UBC9