title: Quantification of growth factors by using a new system for obtaining platelet-rich plasma.
abstract: OBJECTIVE
  To verify the performance of a new method for obtaining platelet-rich plasma, while avoiding contamination of the sample during its processing.
  
  
  METHODS
  Twenty healthy patients were selected, from whom 21 ml of blood was extracted. We then proceeded to study the platelets and growth factors in basal blood after centrifuging the sample by using a new closed system for obtaining platelet-rich plasma (PRP).
  
  
  RESULTS
  After centrifuging the blood sample, double the amount of platelets as that found in basal blood was obtained. Of the four growth factors analyzed, only the factor similar to insulin (IGF) contained the same concentration after the centrifuge process. The platelet-derived growth factor (PDGF) and the vascular growth factor (VGF) were multiplied by six with respect to the basal values and disproportionately increased the levels of the transforming growth factor β (TGF-β).
  
  
  CONCLUSIONS
  The new closed method for obtaining PRP, after avoiding contamination of the sample following its use, offers levels of platelet concentrate and growth factors necessary for regeneration.
authors.affiliation: School of Dentistry, European University of Madrid.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lorente-Pérez-Sierra
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ortega-Aranegui
authors.forename: María
authors.initials: M
authors.lastname: Martín-Ares
authors.forename: Juan
authors.initials: J
authors.lastname: López-Quiles-Martínez
authors.forename: José-Ma
authors.initials: JM
authors.lastname: Martínez-González
mesh_terms: D002498:Centrifugation; D006405:Hematology / Q000379:methods; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D055815:Young Adult
chemical_list: D036341:Intercellular Signaling Peptides and Proteins