title: [The cytochrome bc1 complex in the mitochondrial respiratory chain functions according to the Q cycle hypothesis of Mitchell: the proof using a stochastic approach?].
abstract: The bc1 complex is a central complex in the mitochondrial respiratory chain. It links the electrons transfer from ubiquinol (or coenzyme Q) to cytochrome c and proton translocation across the inner mitochondrial membrane. It is widely agreed that the "Q-cycle mechanism" proposed by Mitchell correctly describes the bc1 complex working. It is based on an unexpected separation of the two electrons coming from the coenzyme Q bound at the Q0 site of the bc1 complex. Using the stochastic approach of Gillespie and the known spatial structure of bc1 complexes with the kinetic parameters described by Moser and Dutton we demonstrated the natural emergence of the Q-cycle mechanism and the quasi absence of short-circuits in the functional dimer of bc1 complex without the necessity to invoke any additional mechanism. This approach gives a framework which is well adapted to the modelling of all oxido-reduction reactions of the respiratory chain complexes, normal or mutant.
authors.affiliation: Inserm U688, Université de Bordeaux 2, 146, rue Léo-Saignat, F 33076, Bordeaux Cedex, France. jpm@u-bordeaux2.fr
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Mazat
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ransac
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D019281:Dimerization; D004579:Electron Transport / Q000502:physiology*; D014450:Electron Transport Complex III / Q000502:physiology*; D006801:Humans; D028361:Mitochondrial Diseases / Q000235:genetics / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D010085:Oxidative Phosphorylation; D011487:Protein Conformation; D013269:Stochastic Processes; D014451:Ubiquinone / Q000502:physiology
chemical_list: D014451:Ubiquinone; D000255:Adenosine Triphosphate; D014450:Electron Transport Complex III