title: An in vitro comparison of a new vinyl chalcogenide and sodium selenate on adenosine deaminase activity of human leukocytes.
abstract: Selenium (Se) is a dietary essential trace element with important biological roles. Sodium selenate (Na(2)SeO(4)) is an inorganic Se compound used in human and animal nutrition that acts as precursor for selenoprotein synthesis. The organoselenium 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one (C(21)H(2)HOSe) is an α,β-unsaturated ketone functionalized vinyl chalcogenide that has been found as a potential tool in organic synthesis. Adenosine deaminase (ADA) is an important enzyme in the degradation of adenine nucleotides. In this study, we investigated the in vitro effects of both Se compounds on ADA activity and cell viability in leukocyte suspension (LS) of healthy donors (n=12). We first observed an inhibition of ADA activity using 0.1 μM of 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one, and an increase in cellular viability when 30 μM were used. However, we did not observe alterations in the presence of sodium selenate. Moreover, both Se compounds did not alter lactate dehydrogenase activity and thiobarbituric acid reactive substance levels. These results suggest that the inhibition of ADA activity caused by α,β-unsaturated ketone may affect the adenosine levels in LS and modulate cell viability, attenuating conditions that involve the activation of the immune system.
authors.affiliation: Health Sciences Center, Federal University of Santa Maria, RS, Brazil.
authors.forename: Luziane Potrich
authors.initials: LP
authors.lastname: Bellé
authors.forename: Paula Eliete Rodrigues
authors.initials: PE
authors.lastname: Bitencourt
authors.forename: Faida Husein
authors.initials: FH
authors.lastname: Abdalla
authors.forename: Robson Brum
authors.initials: RB
authors.lastname: Guerra
authors.forename: Claudia
authors.initials: C
authors.lastname: Funchal
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Moretto
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D000328:Adult; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D018011:Chalcogens / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D064586:Selenic Acid; D018036:Selenium Compounds / Q000737:chemistry / Q000494:pharmacology*; D014753:Vinyl Compounds / Q000737:chemistry / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D018011:Chalcogens; D018036:Selenium Compounds; D014753:Vinyl Compounds; D000243:Adenosine Deaminase; D064586:Selenic Acid