title: Differential effect of TLR2 and TLR4 on the immune response after immunization with a vaccine against Neisseria meningitidis or Bordetella pertussis.
abstract: Neisseria meningitidis and Bordetella pertussis are Gram-negative bacterial pathogens that can cause serious diseases in humans. N. meningitidis outer membrane vesicle (OMV) vaccines and whole cell pertussis vaccines have been successfully used in humans to control infections with these pathogens. The mechanisms behind their effectiveness are poorly defined. Here we investigated the role of Toll-like receptor (TLR) 2 and TLR4 in the induction of immune responses in mice after immunization with these vaccines. Innate and adaptive immune responses were compared between wild type mice and mice deficient in TLR2, TLR4, or TRIF. TRIF-deficient and TLR4-deficient mice showed impaired immunity after immunization. In contrast, immune responses were not lower in TLR2-/- mice but tended even to be higher after immunization. Together our data demonstrate that TLR4 activation contributes to the immunogenicity of the N. meningitidis OMV vaccine and the whole cell pertussis vaccine, but that TLR2 activation is not required.
authors.affiliation: Laboratory of Vaccine Research, Netherlands Vaccine Institute, Bilthoven, The Netherlands.
authors.forename: Floris
authors.initials: F
authors.lastname: Fransen
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Stenger
authors.forename: Martien C M
authors.initials: MC
authors.lastname: Poelen
authors.forename: Harry H
authors.initials: HH
authors.lastname: van Dijken
authors.forename: Betsy
authors.initials: B
authors.lastname: Kuipers
authors.forename: Claire J P
authors.initials: CJ
authors.lastname: Boog
authors.forename: Jos P M
authors.initials: JP
authors.lastname: van Putten
authors.forename: Cécile A C M
authors.initials: CA
authors.lastname: van Els
authors.forename: Peter
authors.initials: P
authors.lastname: van der Ley
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000818:Animals; D001886:Bordetella pertussis / Q000378:metabolism*; D049109:Cell Proliferation; D007073:Immunoglobulin E / Q000276:immunology; D008585:Meningitis, Meningococcal / Q000517:prevention & control*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009345:Neisseria meningitidis / Q000276:immunology*; D010567:Pertussis Vaccine / Q000627:therapeutic use; D013154:Spleen / Q000166:cytology; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D007073:Immunoglobulin E; D010567:Pertussis Vaccine; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C476286:Ticam1 protein, mouse