title: Immobilization of catalase via adsorption on poly(styrene-d-glycidylmethacrylate) grafted and tetraethyldiethylenetriamine ligand attached microbeads.
abstract: Fibrous poly(styrene-d-glycidylmethacrylate) (P(S-GMA)) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface initiated-atom transfer radical polymerization (SI-ATRP). Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The multi-modal ligand attached beads were used for reversible immobilization of catalase. The influences of pH, ionic strength and initial catalase concentration on the immobilization capacities of the P(S-DVB)-g-P(S-GMA)-TEDETA beads have been investigated. Catalase adsorption capacity of P(S-DVB-g-P(S-GMA)-TEDETA beads was found to be 40.8 ± 1.7 mg/g beads at pH 6.5 (with an initial catalase concentration 1.0mg/mL). The K(m) value for immobilized catalase on the P(S-DVB-g-P(S-GMA)-TEDETA beads (0.43 ± 0.02 mM) was found about 1.7-fold higher than that of free enzyme (0.25 ± 0.03 mM). Optimum operational temperature and pH was increased upon immobilization. The same support was repeatedly used five times for immobilization of catalase after regeneration without significant loss in adsorption capacity or enzyme activity.
authors.affiliation: Gazi University, Faculty of Arts and Sciences, Biochemical Processing and Biomaterial Research Laboratory, Teknik Okullar, 06500 Ankara, Turkey. g_bayramoglu@hotmail.com
authors.forename: Gulay
authors.initials: G
authors.lastname: Bayramoglu
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Karagoz
authors.forename: Meltem
authors.initials: M
authors.lastname: Yilmaz
authors.forename: Niyazi
authors.initials: N
authors.lastname: Bicak
authors.forename: M Yakup
authors.initials: MY
authors.lastname: Arica
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D001965:Bromides / Q000737:chemistry; D002374:Catalase / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D008024:Ligands; D008689:Methacrylates / Q000737:chemistry*; D008863:Microspheres; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D001965:Bromides; D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D005029:Ethylenediamines; D007477:Ions; D008024:Ligands; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D002374:Catalase; C007870:glycidyl methacrylate