title: [The study of inhibitory and apoptosis of K562 cells by antisense oligodeoxynucleotides].
abstract: OBJECTIVE
  To study the effect of bcr-abl fusion gene antisense phosphorothioate oligodeoxynucleotide (Aspo) on bcr-abl mRNA and apoptosis of K562 cells.
  
  
  METHODS
  Cells were exposed to Aspo. P210 was measured by Flow Cytometry. Cellular bcr-abl mRNA was detected by RT-PCR mediquantitative analysis. Cell apoptosis was measured by Flow Cytometry and observed by electron microscope (EM).
  
  
  RESULTS
  The P210 was down regulated or completely suppressed after 24h treatment with more than 5 micromol/L Aspo. The decrease of bcr-abl mRNA was about 45%. After incubation 48 h with 10 micromol/L Aspo. Also, 20% - 30% K562 cells were induced to apoptosis at 120 h when the cell number was 1 x 10(4)/ml at the beginning. While the cell number was 1 x 10(5)/ml, the apoptosis rate was 30% after 48 h culture and the typical morphology of apoptosis cell was observed under EM.
  
  
  CONCLUSIONS
  bcr-abl Aspo could inhibit the expression of bcr-abl mRNA and P210. Also,it could induce apoptosis of K562 cells.
authors.affiliation: Fujian Institute of Hematology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, China.
authors.forename: Q Z
authors.initials: QZ
authors.lastname: Shi
authors.forename: L H
authors.initials: LH
authors.lastname: Lu
authors.forename: J D
authors.initials: JD
authors.lastname: Hu
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chen
authors.forename: Y L
authors.initials: YL
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D054735:Phosphorothioate Oligonucleotides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D016376:Oligonucleotides, Antisense; D054735:Phosphorothioate Oligonucleotides; D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl