title: Development of a sensitive non-targeted method for characterizing the wine volatile profile using headspace solid-phase microextraction comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry.
abstract: Future understanding of differences in the composition and sensory attributes of wines require improved analytical methods which allow the monitoring of a large number of volatiles including those present at low concentrations. This study presents the optimization and application of a headspace solid-phase microextraction (HS-SPME) method for analysis of wine volatiles by comprehensive two-dimensional gas chromatography (GC×GC) time-of-flight mass spectrometry (TOFMS). This study demonstrates an important advancement in wine volatile analysis as the method allows for the simultaneous analysis of a significantly larger number of compounds found in the wine headspace compared to other current single dimensional GC-MS methodologies. The methodology allowed for the simultaneous analysis of over 350 different tentatively identified volatile and semi-volatile compounds found in the wine headspace. These included potent aroma compound classes such as monoterpenes, norisoprenoids, sesquiterpenes, and alkyl-methoxypyrazines which have been documented to contribute to wine aroma. It is intended that wine aroma research and wine sensory research will utilize this non-targeted method to assess compositional differences in the wine volatile profile.
authors.affiliation: Separation Science Laboratory, Murdoch University, South Street, Murdoch, WA 6150, Australia.
authors.forename: Anthony L
authors.initials: AL
authors.lastname: Robinson
authors.forename: Paul K
authors.initials: PK
authors.lastname: Boss
authors.forename: Hildegarde
authors.initials: H
authors.lastname: Heymann
authors.forename: Peter S
authors.initials: PS
authors.lastname: Solomon
authors.forename: Robert D
authors.initials: RD
authors.lastname: Trengove
mesh_terms: D000704:Analysis of Variance; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D009812:Odorants / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*; D013729:Terpenes / Q000032:analysis*; D055549:Volatile Organic Compounds / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D013729:Terpenes; D055549:Volatile Organic Compounds