title: In vitro degradation of fluoranthene by bacteria isolated from petroleum sludge.
abstract: An investigation was carried out for in vitro degradation of fluoranthene by four bacterial strains (PSM6, PSM7, PSM10 and PSM11) isolated from the petroleum sludge. Although all the strains registered their growth in MSM with 100 ppm fluoranthene, PSM11 growth was better than other strains. Growth of bacterial strains invariably corresponded to their degradation potential of fluoranthene. After 168 h of incubation, 61% fluoranthene was degraded by PSM11, followed by PSM10 (48%) and PSM6 (42%) and the least was recorded in PSM7 (41%). Besides, 11% loss in fluoranthene was attributed to abiotic factors. Thirty-eight times more activity of catechol 2,3-dioxygenase than catechol 1,2-dioxygenase showed that it played a significant role in fluoranthene degradation. Molecular weight of catechol 2,3-dioxygenase isolated from PSM11 was determined as ∼ 136 kDa by size exclusion chromatography and 34 kDa on denaturing SDS-PAGE, indicating tetrameric nature of the enzyme.
authors.affiliation: Environmental Science Division, National Botanical Research Institute, Lucknow-226001, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Upadhayay
authors.forename: Babita
authors.initials: B
authors.lastname: Kumari
authors.forename: Sadhna
authors.initials: S
authors.lastname: Tiwari
authors.forename: S N
authors.initials: SN
authors.lastname: Singh
authors.forename: P K
authors.initials: PK
authors.lastname: Singh
mesh_terms: D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D050548:Catechol 1,2-Dioxygenase / Q000737:chemistry; D050557:Catechol 2,3-Dioxygenase / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005449:Fluorenes / Q000737:chemistry* / Q000378:metabolism; D066298:In Vitro Techniques; D007501:Iron / Q000737:chemistry; D010578:Petroleum / Q000032:analysis*; D010802:Phylogeny; D012722:Sewage / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013234:Stem Cells; D013997:Time Factors
chemical_list: D005449:Fluorenes; D010578:Petroleum; D012722:Sewage; C007738:fluoranthene; D007501:Iron; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase