title: [Simultaneously optical recording of membrane potential in population vestibular ganglion neurons].
abstract: OBJECTIVE
  To investigate membrane electro-physiological features in vestibular ganglion neuronal population using a voltage-sensitive dye and optical recording technique.
  
  
  METHODS
  Dissociated and cultured mouse vestibular ganglion neurons were stained with an absorption voltage-sensitive dye, RH 155, and were imaged in 16 x 16 elements Photodiode arrays (PDA) optical recording system.
  
  
  RESULTS
  When the cells were depolarized during perfusion with 150 mmol/L potassium solution, optical absorption of the dye that bound to the external surface of neuron membranes increased. The relative ratio (delta I/I) of optical absorption change was 0.23% +/- 0.08% (x +/- s, n = 28). These optical responses were wavelength dependent. Under our experimental conditions, photo toxicity and pharmacological effects of the dye were either absent or insignificant.
  
  
  CONCLUSIONS
  Our results suggest that optical recording provides a new, practical and less toxic method to simultaneously monitor changes in membrane potential from several cultured vestibular ganglion cells.
authors.affiliation: Department of Otolaryngology, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Yang
authors.forename: Si-Chang
authors.initials: SC
authors.lastname: Jiang
authors.forename: Wei-Yan
authors.initials: WY
authors.lastname: Yang
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005456:Fluorescent Dyes; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D014725:Vestibular Nerve / Q000502:physiology*
chemical_list: D005456:Fluorescent Dyes