title: A novel method for ABO genotyping using a DNA chip.
abstract: ABO genotyping is often performed to identify the blood type of decomposed samples, which is difficult to be determined by a serological test. In this study, we developed a simple method for ABO genotyping using a DNA chip. In this method, polymerase chain reaction-amplified and fluorescent-labeled fragments in the ABO gene and primate-specific D17Z1 were hybridized with DNA probes on a chip designed to detect single nucleotide polymorphisms (SNPs) in the ABO gene and part of the D17Z1 sequence. Using blood samples from 42 volunteers and 10 animal species, we investigated whether the chip could be used to detect SNPs in the ABO gene and the D17Z1 sequence. This method was then applied to various forensic samples, and it was confirmed that this method was suitable for the simultaneous analyses of ABO genotyping and species identification. This method fulfills the recent need for the development of rapid and convenient methods for criminal investigations.
authors.affiliation: National Research Institute of Police Science, Chiba, Japan. k-watanabe@nrips.go.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikegaya
authors.forename: Koichi
authors.initials: K
authors.lastname: Hirayama
authors.forename: Hisako
authors.initials: H
authors.lastname: Motani
authors.forename: Hirotaro
authors.initials: H
authors.lastname: Iwase
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kaneko
authors.forename: Hisayo
authors.initials: H
authors.lastname: Fukushima
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akutsu
authors.forename: Koichi
authors.initials: K
authors.lastname: Sakurada
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000818:Animals; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D015342:DNA Probes; D005453:Fluorescence; D053803:Forensic Genetics; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: D000017:ABO Blood-Group System; D015342:DNA Probes