title: Short-term effects of double-layer autologous vein graft on restraint of excessive distension and alleviation of neointimal hyperplasia in a porcine saphenous vein graft model.
abstract: Although the use of external vein graft support seems a promising approach to prevent neointimal hyperplasia and wall thickening in vein grafts, its extensive clinical application still has a long way to go. The aim of this study was to evaluate short-term effects of self-designed double-layer autologous saphenous vein graft on restraining excessive distension of vein graft and alleviating neointimal hyperplasia in a porcine model. Left and right hind femoral arteries of 24 white pigs were randomly divided into an experimental group (double-layer vein graft) and a control group (single-layer vein graft). After 1 h of implantation, then 1, 2, and 4 weeks later, the mean inner diameter of the vein grafts in the experimental group measured by Doppler-ultrasound was 2.7 ± 0.1, 2.8 ± 0.1, 2.9 ± 0.1, and 3.1 ± 0.1 mm, respectively; mean peak blood flow velocity measured by Doppler-ultrasound was 96.7 ± 12.8, 93.7 ± 11.5, 89.4 ± 9.6 and 84.6 ± 10.1 cm/s, respectively, while the mean neointimal thicknesses were 47.1 ± 7.7, 93.7 ± 15.1, and 177.4 ± 25.5 μm at 1, 2 and 4 weeks, respectively. As compared to the control group, inner diameter and neointimal thickness of vein grafts in the experimental group were significantly lower, while mean peak blood flow velocity was significantly higher at 1, 2, and 4 weeks after implantation. The proliferation index in the experimental group was also significantly lower within 4 weeks after implantation. The self-designed double-layer autologous saphenous vein graft restrains early excessive distension of vein graft and alleviates early neointimal hyperplasia.
authors.affiliation: Department of Thoracic Cardiovascular Surgery of Tongji Hospital, Tongji University, 389 Xincun Rd., 200065, Shanghai, People's Republic of China. jiqiang1977@yahoo.com.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ji
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Mei
authors.forename: Xisheng
authors.initials: X
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Jianzhi
authors.initials: J
authors.lastname: Cai
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Sun
authors.forename: Wusha
authors.initials: W
authors.lastname: Dewei
authors.forename: Chunping
authors.initials: C
authors.lastname: Wang
authors.forename: Liangjie
authors.initials: L
authors.lastname: Chi
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000009:adverse effects / Q000295:instrumentation*; D049109:Cell Proliferation; D005263:Femoral Artery / Q000601:surgery; D006083:Graft Occlusion, Vascular / Q000000981:diagnostic imaging / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D006965:Hyperplasia; D007150:Immunohistochemistry; D011474:Prosthesis Design; D012039:Regional Blood Flow; D012501:Saphenous Vein / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000000981:diagnostic imaging / Q000473:pathology / Q000637:transplantation*; D018615:Ultrasonography, Doppler, Color; D014654:Vascular Patency