title: High prevalence of Pneumocystis jiroveci colonization among young HIV-infected patients.
abstract: BACKGROUND
  Pneumocystis colonization in young HIV-infected patients has been poorly studied. The aim of this study was to analyze the prevalence of P jiroveci colonization in a cohort of young HIV-infected patients.
  
  
  METHODS
  We designed a basal cross-sectional study in 20 young HIV-infected patients to determine the prevalence of P jiroveci colonization in oropharyngeal wash samples studied by nested polymerase chain reaction (PCR). Subsequently, patients were followed up during 50 weeks to observe the development of Pneumocystis pneumonia (PCP).
  
  
  RESULTS
  P jiroveci colonization was detected in eight (40%) of the 20 oropharyngeal wash samples. Genotype 85C/248C was the most frequent. After 50 weeks of follow-up, one colonized patient with advanced immunodepression developed PCP.
  
  
  CONCLUSIONS
  We have found a high prevalence of P jiroveci colonization in young HIV-infected patients with a major prevalence of genotype 1 (85C/248C). Further studies are necessary to clarify if Pneumocystis colonization could be a potential risk factor of developing PCP in young HIV infected patients.
authors.affiliation: Instituto de Biomedicina de Sevilla, Hospitales Universitarios Virgen del Rocío/CSIC/Universidad de Sevilla, Clinical and Molecular Epidemiology Seville, Spain. anisoguri@hotmail.com
authors.forename: Sonia
authors.initials: S
authors.lastname: Gutiérrez
authors.forename: Rubén
authors.initials: R
authors.lastname: Morilla
authors.forename: Juan A
authors.initials: JA
authors.lastname: León
authors.forename: Isabel
authors.initials: I
authors.lastname: Martín-Garrido
authors.forename: Laura
authors.initials: L
authors.lastname: Rivero
authors.forename: Vicente
authors.initials: V
authors.lastname: Friaza
authors.forename: Nieves
authors.initials: N
authors.lastname: Respaldiza
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Montes-Cano
authors.forename: Rafael
authors.initials: R
authors.lastname: Terán
authors.forename: Carmen
authors.initials: C
authors.lastname: de la Horra
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology; D000293:Adolescent; D002648:Child; D015897:Comorbidity; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology*; D006801:Humans; D008297:Male; D009960:Oropharynx / Q000382:microbiology*; D045363:Pneumocystis carinii / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000453:epidemiology* / Q000382:microbiology*; D015995:Prevalence; D013030:Spain / Q000453:epidemiology