title: Selection of peptides binding to HCV e2 and inhibiting viral infectivity.
abstract: The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yong-In 449-791, Korea.
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Hong
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Lee
authors.forename: Heejoon
authors.initials: H
authors.lastname: Myung
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D016174:Hepacivirus / Q000187:drug effects* / Q000472:pathogenicity*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000037:antagonists & inhibitors*; D053586:Virus Internalization / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D019151:Peptide Library; D010455:Peptides; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus