title: Diclofenac-loaded Eudragit S100 nanosuspension for ophthalmic delivery.
abstract: In this study, diclofenac-loaded Eudragit S100-based nanosuspension was prepared by nanoprecipitation method and characterised for particle size, morphology, in vitro release, and for its in vivo ocular anti-inflammatory activity. The diclofenac-loaded Eudragit S100 nanosuspension was found to have a particle size of 172 nm, polydispersibility index of 0.14 and zeta potential of -23.7 +/- 6.07 mV, indicating that the nanosuspension is fairly stable. The nanosuspended particles were found to be spherical in shape. The nanosuspension was found to provide a sustained in vitro release, following the Higuchi square-root release kinetics. The results indicated that the nanosuspension released the drug by combination of dissolution and diffusion. The in vivo evaluation of nanosuspension in PGE(2)-induced ocular inflammation in rabbit model revealed a significantly (p  <  0.05) higher inhibition of PGE(2)-induced polymorphonuclear leukocytes migration and lid-closure scores as compared with the aqueous solution of diclofenac.
authors.affiliation: Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125 001, India.
authors.forename: Munish
authors.initials: M
authors.lastname: Ahuja
authors.forename: Avinash S
authors.initials: AS
authors.lastname: Dhake
authors.forename: Surendera K
authors.initials: SK
authors.lastname: Sharma
authors.forename: Dipak K
authors.initials: DK
authors.lastname: Majumdar
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000627:therapeutic use; D002465:Cell Movement / Q000187:drug effects; D011232:Chemical Precipitation; D004008:Diclofenac / Q000008:administration & dosage* / Q000627:therapeutic use; D005123:Eye / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D008297:Male; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D009883:Ophthalmic Solutions / Q000737:chemistry*; D010316:Particle Size; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011817:Rabbits; D014961:X-Ray Diffraction
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D009883:Ophthalmic Solutions; D011109:Polymethacrylic Acids; D004008:Diclofenac; C038300:methylmethacrylate-methacrylic acid copolymer