title: [Effect of PDGF-AA on [Ca2+]i in vascular smooth muscle cells in SHRs].
abstract: OBJECTIVE
  To explore the relationship between proliferation and hypertrophy of vascular smooth muscle cells and PDGF-AA and PDGFR-alpha expression in SHRs and the role of [Ca2+]i in it.
  
  
  METHODS
  Express difference of PDGF-AA, PDGFR-alpha, PDGFR-beta in SHR/WKY-VSMC was observed by Western blot. The effect of Ca2+ inhibitor (nimodipine) on proliferation, hypertrophy and free Ca2+ concentration of SHR-VSMC induced by PDGF-AA was observed by Western blot, [3H] incorporation and fluorescent digital image technique.
  
  
  RESULTS
  PDGF-AA and PDGFR-alpha expression was markedly increased in SHR-VSMC than in WKY-VSMC, but PDGFR-beta was not different in SHR and WKY-VSMC. PDGF-AA-stimulated PCNA expression, [3H] incorporation and [Ca2+]i increasing were observed in SHR-VSMC. Dose-dependent nimodipine-inhibited PCNA expression, [3H] incorporation and [Ca2+]i increasing induced by PDGF-AA also were observed in SHR-VSMC.
  
  
  CONCLUSIONS
  Spontaneously expression increasing of PDGF-AA and PDGFR-alpha in spontaneously hypertension rats (SHRs) may be one of the important factors on vascular reactivity and vascular modeling mediated through proliferation and hypertrophy in SHR-VSMC, and [Ca2+]i play an important role in this process.
authors.affiliation: Department of Cardiology, Xingqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jin
authors.forename: Shan-Jun
authors.initials: SJ
authors.lastname: Zhu
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism
chemical_list: D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D017479:Receptors, Platelet-Derived Growth Factor; D002118:Calcium