title: Resibufogenin administration prevents oxidative stress in a rat model of human preeclampsia.
abstract: OBJECTIVE
  Marinobufagenin (MBG) is a cardiotonic steroid that is increased in preeclampsia. An analog of MBG, resibufogenin (RBG), prevents the development of preeclampsia in a rat model. Oxidative stress is a concomitant of endothelial dysfunction in the latter disorder. The objective of the current studies was to evaluate the status of oxidative stress in a rat model of preeclampsia.
  
  
  METHODS
  We measured the aortic AT(1) receptor expression and urinary excretion of 8-isoprostane (8IP) in rats rendered "preeclamptic" and compared the findings to those obtained in normal pregnant animals, pregnant rats injected with MBG, and preeclamptic rats treated with RBG.
  
  
  RESULTS
  Aortic AT(1) receptor expression and the urinary excretion of 8IP were significantly augmented in "preeclamptic" and MBG-injected pregnant rats compared to normal pregnant animals. RBG prevented evidence of oxidative stress in "preeclamptic" rats.
  
  
  CONCLUSIONS
  MBG is involved in the causation of oxidative stress in our rat model and RBG attenuates this change.
authors.affiliation: Department of Medicine, Division of Nephrology and Hypertension, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, Temple, TX, USA.
authors.forename: Mohammad Nasir
authors.initials: MN
authors.lastname: Uddin
authors.forename: Enoch E
authors.initials: EE
authors.lastname: Agunanne
authors.forename: Darijana
authors.initials: D
authors.lastname: Horvat
authors.forename: Jules B
authors.initials: JB
authors.lastname: Puschett
mesh_terms: D000818:Animals; D002018:Bufanolides / Q000008:administration & dosage* / Q000378:metabolism* / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D018384:Oxidative Stress / Q000187:drug effects*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002018:Bufanolides; C005734:bufogenin; C093896:marinobufagenin