title: Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes.
abstract: The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T. vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome I and II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mapping of the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping.
authors.affiliation: Charles University in Prague, Faculty of Science, Department of Parasitology, Vinicna 7, 12844 Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Zubáčová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Krylov
authors.forename: Jan
authors.initials: J
authors.lastname: Tachezy
mesh_terms: D000953:Antigens, Protozoan / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D005453:Fluorescence; D018628:Gene Dosage; D005819:Genetic Markers; D016678:Genome; D005838:Genotype; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012680:Sensitivity and Specificity; D051102:Serine C-Palmitoyltransferase / Q000235:genetics; D014245:Trichomonas Infections / Q000235:genetics / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D014368:Tryptophanase / Q000235:genetics
chemical_list: D000953:Antigens, Protozoan; D005819:Genetic Markers; D051102:Serine C-Palmitoyltransferase; D014368:Tryptophanase