title: The knockdown of Ha-GRIM-19 by RNA interference induced programmed cell death.
abstract: GRIM-19 (genes associated with retinoid-IFN-induced mortality-19) is a subunit of mitochondrial respiratory complex I in mammalian systems. However, its function in vivo is not really understood. We cloned GRIM-19 and explored its function and hormonal regulation in insect, the cotton bollworm, Helicoverpa armigera. The results showed that Ha-GRIM-19 was highly expressed during the larval stage. Its transcript levels could be upregulated by juvenile hormone (JH) analog methoprene or by methoprene plus 20E. The methoprene-upregulated transcription enhancement of Ha-GRIM-19 was mediated by the transcription factor Ha-Met1, the putative receptor of JH. Other transcription factors Ha-USP1 and Ha-Br-Z2 suppressed the action of methoprene in inducing Ha-GRIM-19 expression, but Ha-Br-Z2 introduced interaction between 20E and methoprene in upregulation of Ha-GRIM-19. The knockdown of Ha-GRIM-19 by RNA interference in larvae and in insect cell line induced programmed cell death. These data imply that Ha-GRIM-19 plays role in keeping the normal cellular growth and it is able to be upregulated by methoprene through putative JH receptor Met.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, 250100, China.
authors.forename: Du-Juan
authors.initials: DJ
authors.lastname: Dong
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Liu
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008726:Methoprene / Q000378:metabolism; D009036:Moths / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011679:Pupa / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034622:RNA Interference
chemical_list: D051017:Apoptosis Regulatory Proteins; D019476:Insect Proteins; D008726:Methoprene