title: Prevalence of human coronaviruses in adults with acute respiratory tract infections in Beijing, China.
abstract: Human coronaviruses (HCoVs) are a common etiological agent of acute respiratory tract infections. HCoV infections, especially those caused by the two HCoVs identified most recently, NL63 and HKU-1, have not been characterized fully. To evaluate the prevalence and clinical presentations of HKU1 and NL63 in adults with acute respiratory tract infections, an investigation of HCoV infections in Beijing, China from 2005 to 2009 was performed by using reverse transcriptase PCR assays and sequencing analysis. Among 8,396 respiratory specimens studied, 87 (1%) clinical samples were positive for HCoVs, of which 50 samples (0.6% of the total) were positive for HCoV-OC43, 15 (0.2%) for HCoV-229E, 14 (0.2%) for HCoV-HKU1, and 8 (0.1%) for HCoV-NL63. The prevalence of HCoV infection in adults exhibited distinct seasonal fluctuations during the study period. In addition, patients positive for HCoV-229E infections were more likely to be co-infected with other respiratory viruses. Enterovirus, rhinovirus, and parainfluenza virus type 3 were the most common viruses found in patients with HCoV infections. The demographic and clinical data present in this study of HCoV infections in adults with acute respiratory tract infections should improve our understanding of the pathogenesis of HCoVs.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering and Dr. Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ren
authors.forename: Richard
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhou
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Guy
authors.initials: G
authors.lastname: Vernet
authors.forename: Gláucia
authors.initials: G
authors.lastname: Paranhos-Baccalà
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D017934:Coronavirus / Q000145:classification* / Q000235:genetics; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000821:virology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D014505:Urban Population