title: [iNOS and AChE expression on guinea pigs cochlea spiral ganglion induced by streptomycin and attenuation by Salvia miltiorrhiza injection].
abstract: OBJECTIVE
  To study the expression of iNOS and AChE on ginea pigs cochlea spiral ganglion induced by streptomycin (SM) and attenuation by salvia miltiorrhiza injection (Chinese Traditional medicine-dansen DS).
  
  
  METHODS
  32 guinea pigs were divided into 4 groups randomly (n=8): control group, SM group, DS + SM group, DS group. SABC immunohistochemical staining and image quantitative analysis technique were used to observe the expression of iNOS and AChE, as well as grey value analysis, and ABR measurements were used to observe ototoxicity.
  
  
  RESULTS
  After 10 days with drugs, the ABR threshold value of SM increased more significantly than that of the control (P < 0.01), while the ABR threshold value of DS+ SM co-treatment increased than the control group, but lower than that of SM group (P < 0.01). The results of immunohistochemical staining implied the expression of iNOS and AChE in SG of SM group were higher than that of control group, and had positive correlate.
  
  
  CONCLUSIONS
  The ABR threshold value increases and the expression of iNOS and AChE strengthen on SM ototoxicity, and has some correlation. DS can attenuate the ototoxicity induced by SM, and has protective function.
authors.affiliation: Department of Physiology, China Medical University, Shengyang 110001, China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Guan
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D006319:Hearing Loss, Sensorineural / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D020011:Protective Agents / Q000494:pharmacology; D011897:Random Allocation; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013136:Spiral Ganglion / Q000187:drug effects* / Q000378:metabolism; D013307:Streptomycin / Q000633:toxicity*
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D052247:Nitric Oxide Synthase Type II; D000110:Acetylcholinesterase; D013307:Streptomycin