title: A novel protein-coding ORF72.2 gene was identified from Marek's disease virus strain CVI988.
abstract: Marek's disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas, which is caused by Marek's disease virus (MDV), but its pathogenic mechanism is still not very clear. Recently, some new progress were achieved in molecular character of MDV. Along with the genomic sequencing of MDV serotype 1, some novel open reading frames (ORFs) were predicted, and ORF72.2 was one of them which have no homologues in other MDV serotypes or in other alphaherpesvirus. In the study, ORF72.2 was firstly identified as a protein-coding gene by the method of reverse transcription polymerase chain reaction (RT-PCR), western blotting and indirect immunofluorescence assay. This study paved the way to conduct further studies to determine whether ORF72.2 plays a role in MDV replication and pathogenicity.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, PR China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Tian
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.forename: Nianli
authors.initials: N
authors.lastname: Zou
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D002645:Chickens; D019084:Fluorescent Antibody Technique, Indirect; D005814:Genes, Viral / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000097:blood / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins