title: Human bocavirus infection in children with acute gastroenteritis in Japan and Thailand.
abstract: A total of 329 fecal specimens, which had been known to be negative for rotavirus, adenovirus, norovirus, sapovirus, and astrovirus, and which were collected from infants and children with acute gastroenteritis in Japan and Thailand during 2005-2008 were screened for human bocavirus (HBoV). HBoV was detected by PCR with a primer pair that amplified the NP1 region of its genome and was genotyped by sequencing of the VP1/VP2 region. Of the 329 samples tested, 6 (1.8%) were positive for HBoV. Of these, five samples were collected from Japan and one sample was from Thailand, and the detection rates of HBoV in each country were 2% and 1.2%, respectively. For the detected HBoV, the capsid VP1/VP2 gene of all HBoV strains was successfully sequenced. Four Japanese HBoV strains studied were clustered into group 1, while the remaining Japanese strain and a unique Thai strain belonged to group 2. No severe acute gastroenteritis associated with HBoV was noted. This study provides better understanding on the epidemiology of HBoV infections in children with acute gastroenteritis in Japan and Thailand.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
authors.forename: Ngan Thi Kim
authors.initials: NT
authors.lastname: Pham
authors.forename: Quang Duy
authors.initials: QD
authors.lastname: Trinh
authors.forename: Wisoot
authors.initials: W
authors.lastname: Chan-It
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nishimura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sugita
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.forename: Masashi
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D000293:Adolescent; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology*; D056661:Human bocavirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology*; D010802:Phylogeny; D013785:Thailand / Q000453:epidemiology
chemical_list: D036022:Capsid Proteins