title: Vitamins E and C may increase collagen turnover by intramuscular fibroblasts. Potential for improved meat quality.
abstract: Vitamins influence collagen metabolism in animals grown for meat. This study investigated whether vitamins E and C regulate collagen turnover in muscle by the balance of effects on the synthesis of collagen and its degradation by secretion of matrix metalloproteinases (MMPs) by bovine intramuscular fibroblasts. Fibroblasts isolated from longissimus dorsi (LD) and semitendinosus (ST) muscle were treated with different concentrations of vitamins. Pro-MMP-2, MMP-2, and total soluble collagen (TSC) synthesis were determined. Vitamins E and C each preferentially increased (P < 0.05) MMP-2 in cells derived from LD relative to those derived from ST. Higher TSC values (P < 0.05) were found for ST cells than for LD cells. Both vitamins may increase collagen turnover exerted by intramuscular connective tissue fibroblasts. These results may have implications in vivo on animal production, as a high rate of collagen turnover may lead to increased collagen solubility in muscles, which can affect meat tenderness.
authors.affiliation: Department of Food Science, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Anangelina C
authors.initials: AC
authors.lastname: Archile-Contreras
authors.forename: Ming C
authors.initials: MC
authors.lastname: Cha
authors.forename: Ira B
authors.initials: IB
authors.lastname: Mandell
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Miller
authors.forename: Peter P
authors.initials: PP
authors.lastname: Purslow
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D008460:Meat / Q000032:analysis* / Q000592:standards; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D011786:Quality Control; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D003094:Collagen; D001205:Ascorbic Acid