title: Identification of genes required for neural-specific glycosylation using functional genomics.
abstract: Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, α1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific α1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating α1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.
authors.affiliation: Research Group of Glycobiology and Glycotechnology, Mitsubishi-kagaku Institute of Life Sciences, Tokyo, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Yamamoto-Hino
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kanie
authors.forename: Wakae
authors.initials: W
authors.lastname: Awano
authors.forename: Kiyoko F
authors.initials: KF
authors.lastname: Aoki-Kinoshita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yano
authors.forename: Shoko
authors.initials: S
authors.lastname: Nishihara
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Ryu
authors.initials: R
authors.lastname: Ueda
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
authors.forename: Satoshi
authors.initials: S
authors.lastname: Goto
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D023281:Genomics; D006031:Glycosylation; D009420:Nervous System / Q000378:metabolism; D009928:Organ Specificity
chemical_list: D029721:Drosophila Proteins