title: Targeted amino-terminal acetylation of recombinant proteins in E. coli.
abstract: One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co-expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.
authors.affiliation: School of Biosciences, University of Kent, Canterbury, United Kingdom.
authors.forename: Matthew
authors.initials: M
authors.lastname: Johnson
authors.forename: Arthur T
authors.initials: AT
authors.lastname: Coulton
authors.forename: Michael A
authors.initials: MA
authors.lastname: Geeves
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Mulvihill
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods; D002352:Carrier Proteins / Q000737:chemistry*; D018797:Cell Cycle Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry*; D014335:Tropomyosin / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; C410737:Cdc8 protein, S pombe; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D011994:Recombinant Proteins; C464787:SPART protein, human; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C492432:Tfs1 protein, S cerevisiae; D014335:Tropomyosin; D000123:Acetyltransferases; C556312:NatB protein, S cerevisiae