title: [Enzymatic hydrolysis of antler and properties of hydrolysates].
abstract: Lyophylized antler powder was hydrolyzed by pepsin and trypsin separately and also simultaneously to give hydrolysates with special physical activities. Complete hydrolysis peptides with MW lower than 1 x 10(3) were collected for assay of angiotensin I-converting enzyme (ACE) inhibitory activity, antioxidant activity and proliferative activity toward UMR-106 osteoblast cells. The results of the experiments revealed that all hydrolysates exhibited potent hydroxyl radical scavenging activity with an IC50 value less than 1 mg/ml which was much lower than the value of 5.5 g x L(-1) for vitamin C. The peptic and peptic tryptic hydrolysates demonstrated strong angiotensin I-converting enzyme (ACE) inhibitory activity. The tryptic hydrolysate increased the proliferation of the UMR-106 cells by 73.43%. The results verified the traditional use of antler in bone-strengthening, anti-aging. The exploratory studies on the ACE inhibitory activity of antler hydrolysates indicated that the hydrolysates might be potentially useful in prevention and treatment of hypertension. Further purification of peptides contributing to the antioxidant activity, angiotensin I-converting enzyme-inhibitory activity and proliferative activity toward osteoblasts from antler hydrolysates is warranted.
authors.affiliation: College of Biological Science and Technology, Fuzhou University, Fuzhou 35018, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zheng
authors.forename: Renkuan
authors.initials: R
authors.lastname: Li
authors.forename: Huilin
authors.initials: H
authors.lastname: Wang
authors.forename: Junming
authors.initials: J
authors.lastname: Zhuang
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ye
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D000999:Antlers / Q000737:chemistry* / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003670:Deer; D010450:Endopeptidases / Q000378:metabolism; D016166:Free Radical Scavengers; D006868:Hydrolysis; D006973:Hypertension / Q000139:chemically induced*; D010434:Pepsin A / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010455:Peptides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D010450:Endopeptidases; C047929:peptidyl dipeptidase 4; D007703:Peptidyl-Dipeptidase A; D014357:Trypsin; D010434:Pepsin A