title: IL-6, TNF-alpha, IL-1-beta arterial wall expression is independent of serum concentration in patients sustaining primary or secondary open vascular reconstructions.
abstract: OBJECTIVE
  The aim of this study was to assess morphological and immunohistochemical differences in arterial wall inflammation during primary and secondary vascular reconstructions.
  
  
  METHODS
  Forty patients with lower extremities ischemia underwent vascular reconstruction: Group I included patients undergoing primary vascular reconstruction with 25 minor arterial reconstructive procedures; Group II included patients undergoing secondary reconstruction due to limb ischemia after at least 12 months since first operation (15). Immunohistochemical expression of IL-1-BETA,IL-6,and TNF-alpha in arterial wall was correlated with serum concentration.
  
  
  RESULTS
  No differences were observed in IL-1-beta, IL-6, TNF-alpha expression among groups (P=0.06, P=0.13, P=0.9). Cytokines expression was not accompanied by the growth of its serum concentrations (P=0.8, P=0.9). IL-6 serum concentration depended on the degree of limb ischemia and was very high in case of critical limb ischemia (P=0.006). Arterial morphology was comparable among the groups.
  
  
  CONCLUSIONS
  IL-6, IL-1-BETA, TNF-alpha expression in the arterial wall is comparable in primary and secondary lesions. Serum concentrations of IL-6, IL-1-BETA, TNF-alpha are independent of its arterial wall expression.
authors.affiliation: Department of General and Vascular Surgery, Poznan University of Medical Sciences, Dluga 1/2, Poznan, Poland. goranm@gazeta.pl
authors.forename: M
authors.initials: M
authors.lastname: Stanisic
authors.forename: W
authors.initials: W
authors.lastname: Majewski
authors.forename: J
authors.initials: J
authors.lastname: Zurawski
authors.forename: K
authors.initials: K
authors.lastname: Lesniewska
authors.forename: P
authors.initials: P
authors.lastname: Majewski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001158:Arteries / Q000276:immunology / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D007511:Ischemia / Q000276:immunology / Q000473:pathology / Q000601:surgery*; D035002:Lower Extremity / Q000098:blood supply*; D008875:Middle Aged; D011044:Poland; D012086:Reoperation; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D014656:Vascular Surgical Procedures
chemical_list: C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha