title: Interactions of Aβ25-35 β-barrel-like oligomers with anionic lipid bilayer and resulting membrane leakage: an all-atom molecular dynamics study.
abstract: Aβ25-35, a proteolytic fragment of the Alzheimer amyloid beta (Aβ) peptide, is produced in the brains of Alzheimer's patients and retains the neurotoxicity of its full-length counterpart. The formation of pores/channels in membranes has been reported as one of the mechanisms responsible for Aβ25-35 toxicity. In addition, it has been proposed that pore/channel might be formed by the aggregation of Aβ25-35 in membranes into a β-barrel structure. However, the structure of the β-barrel and its perturbation on the ordering of lipid bilayer at atomic level remain elusive. In this study, we have investigated the interactions of three types of preformed Aβ25-35 β-barrels (labeled as barrels A, B, and C) with negatively charged palmitoyloleoylphosphatidylglycerol (POPG) lipid bilayers using all-atom molecular dynamics (MD) simulations. Each type of Aβ25-35 β-barrel consists of eight β-strands with positively charged side chains of lysine residues oriented toward the interior or exterior of the barrel. Barrels A, B, and C have respectively an out-of-register mixed parallel-antiparallel (taken from our previous study), in-register mixed parallel-antiparallel, and in-register antiparallel β-strand arrangements. Simulations have been performed by employing the initial configurations where the β-barrels are fully or partially inserted into the bilayer. On the basis of nine independent 150 ns MD runs for the full-insertion system, we found that barrels A and C slightly affect the local ordering of lipid bilayer, while barrel B perturbs the local structure of membrane and even causes membrane leakage for water by forming nanometer-sized hydrophilic pore when lysine residues on its inner side. Two 100 ns MD simulations on partial-insertion system show that partial insertion of Aβ25-35 β-barrel in the bilayer results in a tendency to stay inside for barrel B. These results suggest that barrel B with Lys residues on its inner side is the most likely Aβ25-35 pore structure leading to membrane leakage. Our MD simulations provide significant insight into the atomic resolution structure of Aβ25-35 β-sheet-rich pores and the membrane disruption mechanism induced by Aβ25-35 amyloid pores.
authors.affiliation: State Key Laboratory of Surface Physics, Department of Physics, Fudan University, Shanghai, China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Chang
authors.forename: Yin
authors.initials: Y
authors.lastname: Luo
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guanghong
authors.initials: G
authors.lastname: Wei
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D010446:Peptide Fragments / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D017433:Protein Structure, Secondary; D013696:Temperature
chemical_list: D016229:Amyloid beta-Peptides; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010715:Phosphatidylglycerols; C074636:amyloid beta-protein (25-35); C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol