title: HLA-matched sibling transplantation with G-CSF mobilized PBSCs and BM decreases GVHD in adult patients with severe aplastic anemia.
abstract: BACKGROUND
  Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an effective treatment for severe aplastic anemia (SAA). However, graft failure and graft-versus-host disease (GVHD) are major causes of the early morbidity in Allo-HSCT.
  
  
  METHODS
  To reduce graft failure and GVHD, we treated fifteen patients with SAA using high- dose of HSCT with both G-CSF mobilized PB and BMSCs from HLA-identical siblings to treat patients with SAA.
  
  
  RESULTS
  All patients had successful bone marrow engraftment. Only one patient had late rejection. Median time to ANC greater than 0.5 × 10(9)/L and platelet counts greater than 20 × 10(9)/L was 12 and 16.5 days, respectively. No acute GVHD was observed. The incidence of chronic GVHD was 6.67%. The total three-year probability of disease-free survival was 79.8%.
  
  
  CONCLUSIONS
  HSCT with both G-CSF mobilized PB and BMSCs is a promising approach for heavily transfused and/or allo-immunized patients with SAA.
authors.forename: Zi-Min
authors.initials: ZM
authors.lastname: Sun
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Liu
authors.forename: Liang-Quan
authors.initials: LQ
authors.lastname: Geng
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Yao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Kai-Yang
authors.initials: KY
authors.lastname: Ding
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Han
authors.forename: Hui-Zhi
authors.initials: HZ
authors.lastname: Yang
authors.forename: Bo-lin
authors.initials: BL
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Tong
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Zhu
authors.forename: Zu-Yi
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000328:Adult; D000741:Anemia, Aplastic / Q000628:therapy*; D006086:Graft vs Host Disease / Q000276:immunology / Q000517:prevention & control*; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006680:HLA Antigens / Q000276:immunology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D035781:Siblings; D014019:Tissue Donors
chemical_list: D006680:HLA Antigens; D016179:Granulocyte Colony-Stimulating Factor