title: Endothelin-1 expression by vascular endothelial growth factor in human umbilical vein endothelial cells and aortic smooth muscle cells.
abstract: OBJECTIVE
  This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types.
  
  
  METHODS
  We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells.
  
  
  RESULTS
  In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture.
  
  
  CONCLUSIONS
  VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University, Seoul, Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Seol
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Oh
authors.forename: Hai-Joong
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism*; D000072396:Endothelin-Converting Enzymes; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D019332:Endothelin-1; D006020:Glycopeptides; D042461:Vascular Endothelial Growth Factor A; D019716:Tissue Inhibitor of Metalloproteinase-2; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes; C008890:phosphoramidon