title: IL-10 and IL-10 receptor overexpression in oral giant cell lesions.
abstract: OBJECTIVE
  Central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) occur in the jaws and contain osteoclast-like giant cells and mononuclear cells positive for the macrophage marker CD68. The participation of immune-inflammatory mechanisms has been proposed in the lesions development. As IL-10 is one of the most important anti-inflammatory cytokines and it is also an inhibitory cytokine to macrophage function and bone resorption, the purpose of the present study was to investigate its expression together with its receptor (IL-10Rα) in CGCL and PGCL.
  
  
  METHODS
  Six fragments of CGCL and seven fragments of PGCL were obtained by surgical excision. Frozen specimens were cut and subjected to immunofluorescence staining using fluorescent-labeled anti-CD68, anti-IL-10, and anti-IL-10Rα monoclonal antibodies. Microscopic analyses were performed and the percentage of positive mononuclear and giant cells for each parameter was obtained.
  
  
  RESULTS
  Our results revealed that all giant cells from CGCL and PGCL were CD68+ and IL-10Rα+ and that the majority was also positive for IL-10. More than 50% of the mononuclear cells from both lesions expressed IL-10Rα and the majority of these cells were CD68+ and IL-10+.
  
  
  CONCLUSIONS
  Considering that IL-10 has inhibitory effects on the pathologic processes related to the development of the oral giant cell lesions, the high frequencies of cells producing this cytokine seems contradictory to these lesions growth. Investigation about the production of inflammatory cytokines as well as the IL-10 signaling pathways in oral giant cell lesions is required to elucidate the immunopathology of CGCL and PGCL.
authors.affiliation: Laboratory of Oral Biology, School of Dentistry, Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte-MG, Brazil.
authors.forename: Nárriman-Fátima-Lima
authors.initials: NF
authors.lastname: Syrio
authors.forename: Daniela-Rodrigues
authors.initials: DR
authors.lastname: Faria
authors.forename: Ricardo-Santiago
authors.initials: RS
authors.lastname: Gomez
authors.forename: Kenneth-John
authors.initials: KJ
authors.lastname: Gollob
authors.forename: Walderez-Ornelas
authors.initials: WO
authors.lastname: Dutra
authors.forename: Paulo-Eduardo-Alencar
authors.initials: PE
authors.lastname: Souza
mesh_terms: D000328:Adult; D002648:Child; D005260:Female; D006101:Granuloma, Giant Cell / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000378:metabolism*; D053701:Receptors, Interleukin-10 / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: D053701:Receptors, Interleukin-10; D016753:Interleukin-10