title: Photodynamic therapy with the silicon phthalocyanine pc 4 induces apoptosis in mycosis fungoides and sezary syndrome.
abstract: Our current focus on the effects of Photodynamic Therapy (PDT) using silicon phthalocyanine Pc 4 photosensitizer on malignant T lymphocytes arose due to preclinical observations that Jurkat cells, common surrogate for human T cell lymphoma, were more sensitive to Pc 4-PDT-induced killing than epidermoid carcinoma A431 cells. Mycosis fungoides (MF) as well as Sezary syndrome (SS) are variants of cutaneous T-cell lymphoma (CTCL) in which malignant T-cells invade the epidermis. In this study, we investigated the cytotoxicity of Pc 4-PDT in peripheral blood cells obtained from patients with SS and in skin biopsies of patients with MF. Our data suggest that Pc 4-PDT preferentially induces apoptosis of CD4(+)CD7(-) malignant T-lymphocytes in the blood relative to CD11b(+) monocytes and nonmalignant T-cells. In vivo Pc 4-PDT of MF skin also photodamages the antiapoptotic protein Bcl-2.
authors.affiliation: Department of Dermatology, University Hospitals of Cleveland, Case Western Reserve University, 11100 Euclid Avenue, Lakeside 3500, Cleveland, OH 44106-5028, USA.
authors.forename: Minh
authors.initials: M
authors.lastname: Lam
authors.forename: Yoojin
authors.initials: Y
authors.lastname: Lee
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Hsia
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Morrissey
authors.forename: Chunlin
authors.initials: C
authors.lastname: Yan
authors.forename: Kashif
authors.initials: K
authors.lastname: Azzizudin
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Oleinick
authors.forename: Thomas S
authors.initials: TS
authors.lastname: McCormick
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Cooper
authors.forename: Elma D
authors.initials: ED
authors.lastname: Baron