title: Enamel remineralization by fluoride-releasing materials: proposal of a pH-cycling model.
abstract: This study proposes a pH-cycling model for verifying the dose-response relationship in fluoride-releasing materials on remineralization in vitro. Sixty bovine enamel blocks were selected for the surface microhardness test (SMH1). Artificial caries lesions were induced and surface microhardness test (SMH2) was performed. Forty-eight specimens were prepared with Z 100, Fluroshield, Vitremer and Vitremer ¼ diluted - powder/liquid, and subjected to a pH-cycling model to promote remineralization. After pH-cycling, final surface microhardness (SMH3) was assessed to calculate percent recovery of surface microhardness (%SMHR). Fluoride present in enamel (μg F/mm3) and in the pH-cycling solutions (μg F) was measured. Cross-sectional microhardness was used to calculate mineral content (∆Z). There was no significant difference between Z 100 and control groups on analysis performed on - %SMHR, ∆Z, μg F and mg F/mm3 (p>0.05). Results showed a positive correlation between %SMHR and μg F/mm3 (r=0.9770; p=0.004), %SMHR and μg F (r=0.9939; p=0.0000001), ∆ and μg F/mm3 (r=0.9853; p=0.0002), ∆ and μg F (r=0.9975; p=0.0000001) and between μg F/mm3 and μg F (r=0.9819; p=0.001). The pH-cycling model proposed was able to verify in vitro dose-response relationship of fluoride-releasing materials on remineralization.
authors.affiliation: Department of Pediatric and Social Dentistry.
authors.forename: Eliana
authors.initials: E
authors.lastname: Rodrigues
authors.forename: Alberto Carlos Botazzo
authors.initials: AC
authors.lastname: Delbem
authors.forename: Denise
authors.initials: D
authors.lastname: Pedrini
authors.forename: Luciana
authors.initials: L
authors.lastname: Cavassan
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002327:Cariostatic Agents / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D002417:Cattle; D003188:Composite Resins / Q000737:chemistry; D003731:Dental Caries / Q000473:pathology; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects*; D003764:Dental Materials / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D005899:Glass Ionomer Cements / Q000737:chemistry; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D008903:Minerals / Q000032:analysis; D010895:Pit and Fissure Sealants / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D017001:Tooth Demineralization / Q000503:physiopathology; D014089:Tooth Remineralization / Q000379:methods*; D015040:Zirconium / Q000737:chemistry
chemical_list: D002327:Cariostatic Agents; D003188:Composite Resins; D003764:Dental Materials; D005899:Glass Ionomer Cements; D008903:Minerals; D010895:Pit and Fissure Sealants; D011140:Polyurethanes; C084550:Vitremer; C083568:Z100 composite resin; C086661:Fluoroshield; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D015040:Zirconium; D005459:Fluorides