title: The tightly regulated copper window in yeast.
abstract: The two opposing yeast copper regulators, Ace1 and Mac1, were converted into two fluorescent probes, Ace1-FRET and Mac1-FRET, which selectively and sensitively respond to Cu(+). The Cu(+)-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(i) is maintained in a narrow window inside yeast.
authors.affiliation: Department of Chemistry, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Seraphine V
authors.initials: SV
authors.lastname: Wegner
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.forename: Nick
authors.initials: N
authors.lastname: Hernandez
authors.forename: Chuan
authors.initials: C
authors.lastname: He
mesh_terms: D003300:Copper / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C060256:CUP2 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; C084954:MAC1 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003300:Copper