title: [Effects of glutamine on the colon of mice subjected to colitis gravis].
abstract: OBJECTIVE
  To investigate the effects of glutamine on the colonic mucosa of mice subjected to colitis gravis.
  
  
  METHODS
  64 Kunming mice were divided randomly into 4 groups (n=16): healthy group: animals not subjected to colitis; model group: animals subjected to colitis gravis but without glutamine supplementation; low-Gln group: animals subjected to colitis gravis and with low dose of glutamine supplementation; high-Gln group: animals subjected to colitis gravis and with high dose of glutamine supplementation. Animals belonging to the control, the low-Gln, the high-Gin groups were subjected to coloclysis by HAC to be colitis gravis animals. When the models were established, the healthy and the control groups were given some isotonic Na chloride by intragastric administration. The low-Gln group and the high-Gln group were given the same volume but different concentration of glutamine(low-Gln group--2 mmol x Kg(-1) bw, high-Gln group--2 mmol x Kg(-1) bw) for 7 days. Then the mice were sacrificed, the pathohistological changes of the colon were observed, besides, the content of endotoxin in the blood serum, the level of counteracting oxidation and the activities of MPO of the colon tissue were determined.
  
  
  RESULTS
  The glutamine lessened the pathological injures in the colon and relieved the step up of the content of endotoxin in the blood serum , the step down level of counteracting oxidation and the step up activity of MPO in the colon tissue, which were caused by colitis gravis.
  
  
  CONCLUSIONS
  The glutamine can protect the colon of mice subjected to colitis gravis.
authors.affiliation: College of Veterinary Medicine, Northeast Agricaltural University, Harbin 150030, China.
authors.forename: Jin-min
authors.initials: JM
authors.lastname: Li
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Jia
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D019342:Acetic Acid; D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000473:pathology*; D005260:Female; D005973:Glutamine / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D011897:Random Allocation
chemical_list: D005973:Glutamine; D019342:Acetic Acid