title: [Lysophosphatidic acid acyltransferase β gene expression in newly diagnosed leukemia patients].
abstract: This study was aimed to quantitatively detect the expression level of lysophosphatide acid acyltransferase β (lpaat β) mRNA in leukemia patients so as to provide theoretical basis for the target therapy of lpaat β in leukemia. Real-time fluorescently quantitative PCR was used to detect the relative expression level of lpaat β mRNA to analyze its expression change in various type of leukemia. The results showed that the lpaat β mRNA expression level in acute leukemia (AL) patients was significantly higher than that in normal controls (p < 0.05); lpaat β mRNA expression level in acute myeloid leukemia (AML) patients was significantly higher than that in normal controls (p < 0.05) and was positively correlated with white blood cell count (≥ 20.0 × 10(9)/L) (p < 0.05) and CD34 expression level of leukemia, but was not related with extramedullary infiltration. Except for acute promyelocytic leukemia (APL), the lpaat β mRNA expression level was negatively correlated with chemotherapy sensitivity in chronic myeloid leukemia (AML) patients. lpaat β mRNA expression level in chronic myeloid leukemia (CML) patients was significantly higher than that in normal controls (p < 0.05). lpaat β mRNA expression level in acute lymphoid leukemia (ALL) patients was not higher than that in normal controls (p > 0.05). It is concluded that the lpaat β gene overexpression exists in both AML and CML patients. lpaat β produced by AML cells probably plays an important role in abnormal proliferation and drug-resistance of AML cells.
authors.affiliation: Department of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China. deanzhanrong@yahoo.com.cn
authors.forename: Rong
authors.initials: R
authors.lastname: Zhan
authors.forename: Hao-Bo
authors.initials: HB
authors.lastname: Huang
authors.forename: Shun-Quan
authors.initials: SQ
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002648:Child; D005260:Female; D015870:Gene Expression; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase