title: Immunohistochemical study on the expression of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebellum of the nNOS knock-out(-/-) mice.
abstract: Nitric Oxide (NO) actively participates in the regulation of neuronal intracellular Ca(2+) levels by modulating the activity of various channels and receptors. To test the possibility that modulation of Ca(2+) buffer protein expression level by NO participates in this regulatory effect, we examined expression of calbindin-D28k, calretinin, and parvalbumin in the cerebellum of neuronal NO synthase knock-out (nNOS((-/-))) mice using immunohistochemistry. We observed that in the cerebellar cortex of the nNOS((-/-)) mice, expression of calbindin-D28k and parvalbumin were significantly increased while expression of calretinin was significantly decreased. These results suggest another mechanism by which NO can participate in the regulation of Ca(2+) homeostasis.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jae Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Chung
authors.forename: Yu Jin
authors.initials: YJ
authors.lastname: Cho
authors.forename: Jandi
authors.initials: J
authors.lastname: Kim
authors.forename: Nahee
authors.initials: N
authors.lastname: Kim
authors.forename: Choong Ik
authors.initials: CI
authors.lastname: Cha
authors.forename: Kyeung Min
authors.initials: KM
authors.lastname: Joo