title: High-throughput analysis of gene expression on tissue sections by in situ hybridization.
abstract: Genome-scale sequencing projects, high-throughput RNAi screens, systematic gene targeting, and system-biology-based network predictions all depend on a validation of biological significance in order to understand the relevance of a particular finding. Such validation, for the most part, rests on low-throughput technologies. This article provides protocols that, in combination with suitable instrumentation, make possible a semi-automated analysis of gene expression on tissue sections by means of in situ hybridization. Knowledge of gene expression localization has the potential to aid, and thereby accelerate, the validation of gene functions.
authors.affiliation: Genes and Behavior Department, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany. geichel@gwdg.de
authors.forename: Gregor
authors.initials: G
authors.lastname: Eichele
authors.forename: Graciana
authors.initials: G
authors.lastname: Diez-Roux
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002863:Chromogenic Compounds / Q000378:metabolism; D005629:Frozen Sections / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D035683:MicroRNAs / Q000378:metabolism; D008867:Microtomy / Q000295:instrumentation / Q000379:methods; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D016612:Paraffin Embedding / Q000379:methods; D011786:Quality Control; D015347:RNA Probes / Q000096:biosynthesis
chemical_list: D000906:Antibodies; D002863:Chromogenic Compounds; D035683:MicroRNAs; D015347:RNA Probes; D009580:Nitroblue Tetrazolium; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase