title: Long-term swimming exercise does not modulate the Akt-dependent endothelial nitric oxide synthase phosphorylation in healthy mice.
abstract: Molecular mechanisms by which exercise exerts cardiovascular benefits are poorly understood. Exercise-induced increase of endothelial NO synthase (eNOS) phosphorylation through the protein kinase Akt has been shown to be a key mechanism underlying the beneficial effect of exercise in coronary artery disease patients. We examined whether this protective pathway might also be activated in long-term-exercised healthy mice. C57BL/6 wild-type mice swam for 24 weeks. A group of sedentary animals were used as controls. Aortic levels of total protein kinase Akt (protein kinase B), phosphorylated Akt at ser473 (p-Akt), total eNOS, phosphorylated eNOS at Ser1177 (p-eNOS), and PECAM-1 (platelet endothelial cell adhesion molecule-1) were assessed by Western blotting. Protein expressions of Akt, p-Akt, eNOS, p-eNOS, and PECAM-1 were not modulated by 24 weeks of exercise. The Akt-dependent eNOS phosphorylation did not seem to be a primary molecular adaptation in response to long-term exercise in healthy mice.
authors.affiliation: Lausanne University Hospital, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland. Maxime.Pellegrin@chuv.ch
authors.forename: Maxime
authors.initials: M
authors.lastname: Pellegrin
authors.forename: Carole
authors.initials: C
authors.lastname: Miguet-Alfonsi
authors.forename: Alain
authors.initials: A
authors.lastname: Berthelot
authors.forename: Lucia
authors.initials: L
authors.lastname: Mazzolai
authors.forename: Pascal
authors.initials: P
authors.lastname: Laurant
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D011897:Random Allocation; D013550:Swimming / Q000502:physiology*; D013997:Time Factors
chemical_list: D052250:Nitric Oxide Synthase Type III; D051057:Proto-Oncogene Proteins c-akt