title: Preparation, characterization and biocompatibility of electrospinning heparin-modified silk fibroin nanofibers.
abstract: In this study, the electrospun silk fibroin nanofibrous scaffolds were modified with heparin by grafting after plasma treatment and blending electrospinning. Morphology, microstructure, chemical composition and grafting efficiency of the heparin-modified silk fibroin nanofibrous scaffolds were characterized to evaluate the effect of modification by means of scanning electron microscopy (SEM), Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectrometer (XPS). The results showed that the heparin was successfully introduced to the silk fibroin nanofibrous scaffolds by both the two kinds of modification, and there was a hydrogen bonding between the silk fibroin and heparin. Moreover, the hydrophilicity, O-containing groups and negative charge density of the heparin-modified scaffolds were enhanced. In vitro coagulation time tests showed that the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) of the heparin-modified scaffolds were much higher than those of the pure silk fibroin scaffolds. L929 fibroblasts and EVCs spread and proliferated better on the heparin-modified scaffolds than on the pure silk fibroin scaffolds. Macrophages, neutrophils and lymphocytes were not observed in the heparin-modified scaffolds, which indicated that the modified scaffolds could induce minor inflammation in vivo. The results indicated that the electrospun heparin-modified silk fibroin nanofibrous scaffolds could be considered as ideal candidates for tissue engineering scaffolds.
authors.affiliation: College of Textile and Clothing Engineering, Soochow University, 178 East Ganjiang Road, Suzhou 215021, China.
authors.forename: Shudong
authors.initials: S
authors.lastname: Wang
authors.forename: Youzhu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Dong
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002478:Cells, Cultured; D005349:Fibroins / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D020007:Implants, Experimental; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014783:Viscosity / Q000187:drug effects; D014914:Whole Blood Coagulation Time
chemical_list: D001672:Biocompatible Materials; D006493:Heparin; D005349:Fibroins