title: [Effect of intracerebroventricular injection of rAAV-HIF-1α on hippocampal neuronal apoptosis in a rat model of Alzheimer disease].
abstract: OBJECTIVE
  To observe the effect of intracerebroventricular injection of rAAV-HIF-1α on hippocampal neuronal apoptosis in a rat model of Alzheimer disease (AD).
  
  
  METHODS
  Thirty-two male SD rats (250-300 g) were randomized into 4 groups (n=8), including the normal control group without any treatment, AD model group with right intracerebroventricular injection of 2 µl Aβ25-35 (10 mg/m1), sham-operated group with right intracerebroventricular injection of 2 µl normal saline, and AD+ rAAV-HIF-1α group with right intracerebroventricular injection of 10 µl rAAV-HIF-1a (1×10¹² v.g./m1) one week after Aβ25-35 injection. The rats were sacrificed to detect the expression of HIF-1α and apoptosis of hippocampal neurons 5 weeks after Aβ25-35 or saline injection.
  
  
  RESULTS
  Western blotting showed that the expression of HIF-1α was significantly higher in AD+rAAV-HIF-1α group (451.59±34.39) than in normal control group (229.05±41.28) and sham-operated group (216.29±37.08) (P<0.05) without significant difference between the latter two groups. The apoptotic ratio of the hippocampal neurons was significantly higher in AD model group ([19.49±2.59]%) than in normal control group ([5.41±0.75]%) and sham-operated group ([5.28±0.66]%) in (P<0.05), and intracerebroventricular injection of rAAV-HIF-1α resulted in a significant reduction of the apoptotic ratio in the AD rats ([12.07±2.06]%) (P<0.05).
  
  
  CONCLUSIONS
  Intracerebroventricular injection of rAAV-HIF-1α can inhibit hippocampal neuronal apoptosis in the rat model of AD.
authors.affiliation: Hebei Chemical and Pharmaceutical Vocational Technology College, Shijiazhuang, China. sun3299@sohu.com
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Sun
authors.forename: Hong-qiang
authors.initials: HQ
authors.lastname: Hao
authors.forename: Wei-na
authors.initials: WN
authors.lastname: Kong
authors.forename: Ling-yun
authors.initials: LY
authors.lastname: Liu
authors.forename: Xi-qing
authors.initials: XQ
authors.lastname: Chai
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000628:therapy*; D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D006624:Hippocampus / Q000166:cytology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D020547:Lateral Ventricles; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit