title: Endothelium-dependent vasodilation in human mesenteric artery is primarily mediated by myoendothelial gap junctions intermediate conductance calcium-activated K+ channel and nitric oxide.
abstract: Myoendothelial microdomain signaling via localized calcium-activated potassium channel (K(Ca)) and gap junction connexins (Cx) is critical for endothelium-dependent vasodilation in rat mesenteric artery. The present study determines the relative contribution of NO and gap junction-K(Ca) mediated microdomain signaling to endothelium-dependent vasodilation in human mesenteric artery. The hypothesis tested was that such activity is due to NO and localized K(Ca) and Cx activity. In mesenteric arteries from intestinal surgery patients, endothelium-dependent vasodilation was characterized using pressure myography with pharmacological intervention. Vessel morphology was examined using immunohistochemical and ultrastructural techniques. In vessel segments at 80 mm Hg, the intermediate (I)K(Ca) blocker 1-[(2-chlorophenyl)diphenyl-methyl]-1H-pyrazole (TRAM-34; 1 μM) inhibited bradykinin (0.1 nM-3 μM)-induced vasodilation, whereas the small (S) K(Ca) blocker apamin (50 and 100 nM) had no effect. Direct IK(Ca) activation with 1-ethyl-2-benzimidazolinone (1-EBIO; 10-300 μM) induced vasodilation, whereas cyclohexyl-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-pyrimidin-4-yl]-amine (1-30 μM), the SK(Ca) activator, failed to dilate arteries, whereas dilation induced by 1-EBIO (10-100 μM) was blocked by TRAM-34. Bradykinin-mediated vasodilation was attenuated by putative gap junction block with carbenoxolone (100 μM), with remaining dilation blocked by N-nitro l-arginine methyl ester (100 μM) and [1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one] (10 μM), NO synthase and soluble guanylate cyclase blockers, respectively. In human mesenteric artery, myoendothelial gap junction and IK(Ca) activity are consistent with Cx37 and IK(Ca) microdomain expression and distribution. Data suggest that endothelium-dependent vasodilation is primarily mediated by NO, IK(Ca), and gap junction Cx37 in this vessel. Myoendothelial microdomain signaling sites are present in human mesenteric artery and are likely to contribute to endothelium-dependent vasodilation via a mechanism that is conserved between species.
authors.affiliation: Department of Pharmacology, School of Medical Sciences, University of New South Wales, Sydney, NSW, Australia.
authors.forename: Preet S
authors.initials: PS
authors.lastname: Chadha
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Matt
authors.initials: M
authors.lastname: Rikard-Bell
authors.forename: Sevvandi
authors.initials: S
authors.lastname: Senadheera
authors.forename: Lauren
authors.initials: L
authors.lastname: Howitt
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Bertrand
authors.forename: T Hilton
authors.initials: TH
authors.lastname: Grayson
authors.forename: Timothy V
authors.initials: TV
authors.lastname: Murphy
authors.forename: Shaun L
authors.initials: SL
authors.lastname: Sandow
mesh_terms: D017630:Connexins / Q000502:physiology; D045462:Endothelium-Dependent Relaxing Factors / Q000502:physiology*; D005260:Female; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000502:physiology*; D008297:Male; D008638:Mesenteric Arteries / Q000502:physiology*; D008875:Middle Aged; D009569:Nitric Oxide / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology; D000097006:Gap Junction alpha-4 Protein
chemical_list: D017630:Connexins; D045462:Endothelium-Dependent Relaxing Factors; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; D009569:Nitric Oxide; D000097006:Gap Junction alpha-4 Protein