title: Comparison of hyperbaric oxygen and medical ozone therapies in a rat model of experimental distal colitis.
abstract: OBJECTIVE
  Previous studies have shown that hyperbaric oxygen (HBO) is effective in reducing the severity of acute distal colitis (ADC). Ozone therapy (OT) reduces inflammation in several pathological conditions. We aimed to compare the effects of HBO therapy and OT in an experimental ADC rat model.
  
  
  METHODS
  Forty rats were randomly divided into four groups: Sham, ADC, ADC + HBO, and ADC + OT. Rats in the sham group were given isotonic saline. In the remaining groups, ADC was created by intracolonic administration of 4% acetic acid. No treatment was given to the ADC group. The rats in the ADC + HBO and ADC + OT groups were given HBO and ozone treatments, respectively. The administration of acetic acid caused an inflammatory response in all animals. Distal colons and blood samples were obtained.
  
  
  RESULTS
  The histopathological score was significantly higher in the ADC group compared to the other groups. The histopathological scores in the ADC + HBO and ADC + OT groups were significantly lower compared to the ADC group (both p < 0.001). The most pronounced therapeutic effect was observed in the ADC + OT group. Malondialdehyde and neopterin levels and superoxide dismutase and glutathione peroxidase activities in the ADC group were significantly higher compared to the other groups (p < 0.001).
  
  
  CONCLUSIONS
  Our data showed that the therapeutic effect of OT is more pronounced than that of HBO therapy. Its possible effect is by means of decreasing inflammation, edema, and oxidative stress. These findings also suggest that it is possible to improve the outcome of ADC by using ozone therapy as an adjuvant therapy.
authors.affiliation: Department of General Surgery, Gulhane Military Medical Academy, Ankara, Turkey. ozcanaltinel@gmail.com
authors.forename: Ozcan
authors.initials: O
authors.lastname: Altinel
authors.forename: Seref
authors.initials: S
authors.lastname: Demirbas
authors.forename: Erdinc
authors.initials: E
authors.lastname: Cakir
authors.forename: Halil
authors.initials: H
authors.lastname: Yaman
authors.forename: Ismail H
authors.initials: IH
authors.lastname: Ozerhan
authors.forename: Eyup
authors.initials: E
authors.lastname: Duran
authors.forename: Tuncer
authors.initials: T
authors.lastname: Cayci
authors.forename: Emin O
authors.initials: EO
authors.lastname: Akgul
authors.forename: Nail
authors.initials: N
authors.lastname: Ersoz
authors.forename: Bulent
authors.initials: B
authors.lastname: Uysal
authors.forename: Bulent
authors.initials: B
authors.lastname: Kurt
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yasar
authors.forename: Sukru
authors.initials: S
authors.lastname: Oter
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Peker
mesh_terms: D000818:Animals; D003092:Colitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019798:Neopterin / Q000097:blood; D018384:Oxidative Stress; D010126:Ozone / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D010126:Ozone; D019798:Neopterin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase