title: Hepatoprotective and TNF-α inhibitory activity of Zosima absinthifolia extracts and coumarins.
abstract: Zosima absinthifolia (ZA) extracts and the coumarins (+)-columbianadin and (-)-deltoin were evaluated for their potential hepatoprotective and antiinflamatory effects in a CCl(4)-induced hepatotoxicity assay in rats and by the inhibition of TNF-α production on LPS-stimulated THP-1 macrophages, respectively. Both the ZA extracts and the coumarins showed hepatoprotective activity confirmed by monitoring the ALT/AST levels and by histopathological examination. The antiinflamatory activity, proved by the inhibition of TNF-α production, was found to be higher for the n-hexane root extract than for coumarins, suggesting synergic potential of the extract. The concentration of (-)-deltoin and (+)-columbianadin in extracts was determined by HPLC analysis.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey. bahadir-ozlem@hotmail.com
authors.forename: Ozlem
authors.initials: O
authors.lastname: Bahadır
authors.forename: Gülçin Saltan
authors.initials: GS
authors.lastname: Çitoğlu
authors.forename: Hanefi
authors.initials: H
authors.lastname: Ozbek
authors.forename: Stefano
authors.initials: S
authors.lastname: Dall'Acqua
authors.forename: Jan
authors.initials: J
authors.lastname: Hošek
authors.forename: Karel
authors.initials: K
authors.lastname: Smejkal
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D019661:Apiaceae / Q000737:chemistry*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002251:Carbon Tetrachloride; D002460:Cell Line; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D003374:Coumarins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D004357:Drug Synergism; D005260:Female; D008070:Lipopolysaccharides; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D000893:Anti-Inflammatory Agents; D003374:Coumarins; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase