title: Continuous micro-production using enzymatic reaction and online monitoring.
abstract: A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit C, controlled-pore glass (CPG), and Sepharose.
authors.affiliation: Ecole d'ingénieurs et d'architectes de Fribourg Boulevard de Pérolles 80.
authors.forename: Regula
authors.initials: R
authors.lastname: Moser
authors.forename: Thierry
authors.initials: T
authors.lastname: Chappuis
authors.forename: Ennio
authors.initials: E
authors.lastname: Vanoli
authors.forename: Simon
authors.initials: S
authors.lastname: Crelier
authors.forename: Olivier
authors.initials: O
authors.lastname: Naef
mesh_terms: D055162:Biocatalysis; D004798:Enzymes / Q000378:metabolism*; D044085:Microfluidics / Q000295:instrumentation*
chemical_list: D004798:Enzymes