title: Construction of uniformly sized pseudo template imprinted polymers coupled with HPLC-UV for the selective extraction and determination of trace estrogens in chicken tissue samples.
abstract: To assess the potential risks associated with the environmental exposure of steroid estrogens, a novel highly efficient and selective estrogen enrichment procedure based on the use of molecularly imprinted polymer has been developed and evaluated. Herein, analogue of estrogens, namely 17-ethyl estradiol (EE(2)) was used as the pseudo template, to avoid the leakage of a trace amount of the target analytes. The resulting pseudo molecularly imprinted polymers (PMIPs) showed large sorption capacity, high recognition ability and fast binding kinetics for estrogens. Moreover, using these imprinted particles as dispersive solid-phase extraction (DSPE) materials, the amounts of three estrogens (E(1), E(2) and E(3)) which were detected by HPLC-UV from the chicken tissue samples were 0.28, 0.31 and 0.17 μg g(-1), and the recoveries were 72.5-78.7%, 90.3-95.2% and 80.5-83.6% in spiked chicken tissue samples with RSD <7%, respectively. All these results reveal that EE(2)-PMIPs as DSPE materials coupled with HPLC-UV could be applied to the highly selective separation and sensitive determination of trace estrogens in chicken tissue samples.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Hanzhong Road 140, Nanjing 210029, PR China. jsntwsh@163.com
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.forename: Meijuan
authors.initials: M
authors.lastname: Ding
authors.forename: Lihua
authors.initials: L
authors.lastname: Yuan
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Wen
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Lina
authors.initials: L
authors.lastname: Chen
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhou
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D002138:Calibration; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000737:chemistry; D004967:Estrogens / Q000032:analysis* / Q000378:metabolism; D005782:Gels; D008460:Meat / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D053758:Nanoparticles; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012822:Silicon Dioxide; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004967:Estrogens; D005782:Gels; D011108:Polymers; D004958:Estradiol; D012822:Silicon Dioxide