title: [Determination of plasma concentration of N-methylcantharidimide by HPLC and its pharmacokinetics after intravenous administration in dogs].
abstract: OBJECTIVE
  To establish a HPLC method for determination of N-methylcantharidimide in dogs' plasma and to study the pharmacokinetics of N-methylcantharidimide in dogs'.
  
  
  METHODS
  The plasma samples were extracted by methanol. The acetonitrile and the purified water composed mobile phase. The flow rate was 0. 7 mL x min(-1), ultraviolet detection wavelength was at 212 nm.
  
  
  RESULTS
  The calibration curve was linear over the range from 0.01-10.0 mg x L(-1) with a correlation coefficiency of 0.996 3. The lower limit of quantitation was 0.01 mg x L(-1). The mean recovery was 92.3%. the relative standard deviation (RSD) of intra-day and inter-day were all less than 10%. After intravenous administration of N-methylcantharidimide with 3 dosages of 10, 15, 20 mg x kg(-1) to dogs, the corresponding distribution half-livers (t1/2alpha) were 1.8, 2.1, 1.7 min, and the elimination half-lives (t1/2beta) were 144,139, 146 min, respectively.
  
  
  CONCLUSIONS
  This method is convenient, accurate and reliable. It can be used for determination of N-methylcantharidimide in dogs' plasma and pharmacokinetic studies.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. wxb7516@yahoo.com.cn
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wu
authors.forename: Xianqin
authors.initials: X
authors.lastname: Luo
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002193:Cantharidin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D054833:Isoindoles / Q000008:administration & dosage / Q000097:blood*; D008297:Male
chemical_list: D054833:Isoindoles; C059315:cantharidin methylimide; D002193:Cantharidin