title: A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples.
abstract: BACKGROUND
  Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis.
  
  
  METHODS
  In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis.
  
  
  RESULTS
  The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis.
  
  
  CONCLUSIONS
  The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.
authors.affiliation: Program in Experimental Pathology, Department of Pathological Sciences, Londrina State University, Londrina, PR, Brazil.
authors.forename: Andrea Cristine
authors.initials: AC
authors.lastname: Koishi
authors.forename: Débora Fonseca
authors.initials: DF
authors.lastname: Vituri
authors.forename: Pedro Sebastião Raimundo
authors.initials: PS
authors.lastname: Dionízio Filho
authors.forename: Alexandre Augusto
authors.initials: AA
authors.lastname: Sasaki
authors.forename: Maria Sueli Soares
authors.initials: MS
authors.lastname: Felipe
authors.forename: Emerson José
authors.initials: EJ
authors.lastname: Venancio
mesh_terms: D000328:Adult; D004271:DNA, Fungal / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000175:diagnosis* / Q000382:microbiology; D010228:Paracoccidioides / Q000235:genetics* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal