title: Genome instability in the context of chromatin structure and fragile sites.
abstract: Genomes are exposed to various external stimuli that induce DNA damage in the form of single- or double-stranded DNA breaks. Fragile sites in the human genome are sensitive to genotoxic stress and, when not appropriately repaired, are responsible for chromosomal aberrations, including the gene amplifications observed in a variety of tumors. Moreover, when DNA lesions from different chromosomes are in close proximity and not repaired, the probability of chromosome translocations is greatly increased. These events can be induced by ionizing radiation that, in a majority of cells, induces a G2/M cell cycle arrest and is characterized by the repositioning of many tumor-related genes closer to the nuclear interior. On the basis of this knowledge, we review functional and structural aspects of chromosomal rearrangements and the DNA repair machinery.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic. bartova@ibp.cz
authors.forename: Eva
authors.initials: E
authors.lastname: Bartova
authors.forename: Gabriela
authors.initials: G
authors.lastname: Galiova
authors.forename: Sona
authors.initials: S
authors.lastname: Legartova
authors.forename: Lenka
authors.initials: L
authors.lastname: Stixova
authors.forename: Alzbeta
authors.initials: A
authors.lastname: Jugova
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kozubek
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics*; D043283:Chromosome Fragile Sites; D042822:Genomic Instability; D006801:Humans
chemical_list: D002843:Chromatin