title: Fermentation and microencapsulation of the nematophagous fungus Hirsutella rhossiliensis in a novel type of hollow beads.
abstract: In this work, fermentation and formulation aspects of the nematophagous fungus Hirsutella rhossiliensis BBA were investigated. When incubated in 2% (w/w) glucose and 0.5% (w/w) yeast extract medium in a 1-L Erlenmeyer flask without baffles, heavy pellet formation was observed. Only 40% of the mycelium had a size less than 500 μm. When a flask with three baffles was used, the portion of mycelium <500 μm rose to 95%. In the next step, the influence of aeration rate and stirrer speed on production of finely dispersed mycelium in a stirred tank reactor was investigated. The best fermentation results were obtained at 0.4 vvm and 400 rpm stirrer speed with 90% mycelium <500 μm and 5 g/L biomass. Then, mycelium was microencapsulated in hollow beads based on sulfoethylcellulose (SEC). Experiments on the capsule nutrient reservoir showed that 15% (w/w) corn gluten and 0.5% (w/w) yeast extract could be replaced with 3% (w/w) autoclaved baker's yeast which was never used as capsule additive before. Radial growth of mycelium out of dried hollow beads containing 1% (w/w) biomass and 3% (w/w) baker's yeast was faster than for alginate beads containing equivalent amounts of biomass and yeast indicating a higher bio-control potential.
authors.affiliation: Department of Engineering and Mathematics, University of Applied Sciences, Bielefeld, Germany. anant.patel@fh-bielefeld.de
authors.forename: Anant V
authors.initials: AV
authors.lastname: Patel
authors.forename: Desiree
authors.initials: D
authors.lastname: Jakobs-Schönwandt
authors.forename: Thomas
authors.initials: T
authors.lastname: Rose
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Vorlop
mesh_terms: D018914:Cells, Immobilized / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004339:Drug Compounding; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006999:Hypocreales / Q000378:metabolism*; D008863:Microspheres
chemical_list: D003470:Culture Media; D005947:Glucose