title: [Effect of protooncogene c-myb on progesterone-induced mouse germinal vesicle stage oocyte maturation in vitro].
abstract: OBJECTIVE
  To investigate the effects of c-myb on progesterone-induced mouse germinal vesicle(GV) stage denuded oocyte (DO) maturation in vitro.
  
  
  METHODS
  We used mouse GV stage oocyte cultured with special concentration progesterone, or/and antisense c-myb ODN, or/and db-cAMP, or/and heparin for 24 h, and observed oocyte maturation and analysed the relationship among them.
  
  
  RESULTS
  We cultured DO in the medium 199 for 24 h, and found 10 micromol/L progesterone had more significant effect than 5 micromol/L progesterone (2 h GVBD% P < 0.05, 8 h PB 1% P < 0.05), but had not more significant effect than 20 micromol/L progesterone. We found that 16 micromol/L antisense c-myb ODN significantly inhibited progesterone (10 micromol/L)-induced mouse germinal vesicle stage oocyte maturation in vitro (2 h GVBD% P < 0.05, 8 h PBI% P < 0.01). 1 x 10(-4) micromol/L dbcAMP, 100 microg/ml heparin could single significantly inhibited progesterone-induced mouse GV stage oocyte maturation in vitro (2 h PBI% all P < 0.01, 8 h PBI% all P < 0.01), and could enhanced the inhibition of 16 micromol/L antisense c-myb ODN (2 h GVBD% all P < 0.01, 8h PBI% all P < 0.01).
  
  
  CONCLUSIONS
  Progesterone, protooncogene c-myb,cAMP and calcium all pay important role in regulating oocyte maturation and the mechanism of progesterone, cAMP and calcium in regulating oocyte maturation may be through the expression of protooncogene c-myb.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zheng
authors.forename: Yue-hui
authors.initials: YH
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020570:Genes, myb; D008540:Meiosis; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects*; D009866:Oogenesis; D011374:Progesterone / Q000494:pharmacology*
chemical_list: D011374:Progesterone