title: [Expression and purification of EBV-LMP2 protein].
abstract: OBJECTIVE
  To obtain a second Epstein-Barr virus membrane protein (LMP2) in insect cells.
  
  
  METHODS
  The full length EBV-LMP2 gene was inserted into baculovirus expression transfer vector pFastBac HT B to obtain the recombinant baculoviruses Bac-LMP2. And generation of recombinant baculoviruses was followed by transfection of the recombinant Bac-LMP2 into insect cells, then the recombinant LMP2 protein was recognized by SDS-PAGE and western blot. The expressed LMP2 protein was purified by one step with Ni-NTA metal chelation chromatography.
  
  
  RESULTS
  The expressed LMP2 protein was confirmed by SDS-PAGE and western blot. The purity of purified LMP2 protein is up to 86% by HPLC analysis.
  
  
  CONCLUSIONS
  The EBV-LMP2 was expressed in insect cells, and the purified LMP2 protein was obtained.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Du
authors.forename: Song-Mei
authors.initials: SM
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000472:pathogenicity*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007313:Insecta / Q000166:cytology; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins