title: IL-4 and IL-13 alter plasmacytoid dendritic cell responsiveness to CpG DNA and herpes simplex virus-1.
abstract: Human plasmacytoid dendritic cells (pDCs) are found in skin lesions in a wide variety of diseases. The role of the microenvironment in these lesions on the function of human pDCs remains elusive. We sought to determine the effect of T(h)2 cytokines on the ability of human pDCs to respond to CpG oligodeoxynucleotides (ODNs) and herpes simplex virus in vitro. In this study, we found that the T(h)2 cytokines, IL-4 and IL-13, modulate Toll-like receptor 9 (TLR-9)- and herpes simplex virus-induced pDC phenotype and enhance the ability of these cells to induce allogeneic T-cell responses. Moreover, T(h)2 cytokines impaired TLR-9-induced secretion of inflammatory cytokines and chemokines. Taken together, these results demonstrate that T(h)2 cytokines are involved in the modulation of pDC function and responsiveness to bacterial- and viral-derived stimuli.
authors.affiliation: Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors.forename: Jurjen
authors.initials: J
authors.lastname: Tel
authors.forename: Ruurd
authors.initials: R
authors.lastname: Torensma
authors.forename: Carl G
authors.initials: CG
authors.lastname: Figdor
authors.forename: I Jolanda M
authors.initials: IJ
authors.lastname: de Vries
mesh_terms: D058621:Blood Buffy Coat / Q000166:cytology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000821:virology; D006561:Herpes Simplex / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D018793:Interleukin-13 / Q000276:immunology* / Q000378:metabolism; D015847:Interleukin-4 / Q000276:immunology* / Q000378:metabolism; D053662:Interleukin-4 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D009838:Oligodeoxyribonucleotides / Q000276:immunology* / Q000494:pharmacology; D018418:Th2 Cells / Q000276:immunology / Q000821:virology; D051217:Toll-Like Receptor 9 / Q000276:immunology / Q000378:metabolism
chemical_list: C408982:CPG-oligonucleotide; C508598:IL4 protein, human; C504000:IL4R protein, human; D018793:Interleukin-13; D053662:Interleukin-4 Receptor alpha Subunit; D009838:Oligodeoxyribonucleotides; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D015847:Interleukin-4