title: Prediction of putative protein interactions through evolutionary analysis of osmotic stress response in the model yeast Saccharomyces cerevisae.
abstract: The osmotic stress response signalling pathway of the model yeast Saccharomyces cerevisae is crucial for the survival of cells under osmotic stress, and is preserved to varying degrees in other related fungal species. We apply a method for inference of ancestral states of characteristics over a phylogeny to 17 fungal species to infer the maximum likelihood estimate of presence or absence in ancestral genomes of genes involved in osmotic stress response. The same method allows us furthermore to perform a statistical test for correlated evolution between genes. Where such correlations exist within the osmotic stress response pathway of S. cerevisae, we have used this in order to predict and subsequently test for the presence of physical protein-protein interactions in an attempt to detect novel interactions. Finally we assess the relevance of observed evolutionary correlations in predicting protein interactions in light of the experimental results. We do find that correlated evolution provides some useful information for the prediction of protein-protein interactions, but that these alone are not sufficient to explain detectable patterns of correlated evolution.
authors.affiliation: Centre for Bioinformatics, Division of Molecular Biosciences, Imperial College London, London SW72AZ, United Kingdom. thomas.thorne@imperial.ac.uk
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Thorne
authors.forename: Hsueh-Lui
authors.initials: HL
authors.lastname: Ho
authors.forename: Maxime
authors.initials: M
authors.lastname: Huvet
authors.forename: Ken
authors.initials: K
authors.lastname: Haynes
authors.forename: Michael P H
authors.initials: MP
authors.lastname: Stumpf
mesh_terms: D005075:Biological Evolution; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D009995:Osmosis; D010802:Phylogeny; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins