title: Concerted changes in gene expression and cell physiology of the cyanobacterium Synechocystis sp. strain PCC 6803 during transitions between nitrogen and light-limited growth.
abstract: Physiological adaptation and genome-wide expression profiles of the cyanobacterium Synechocystis sp. strain PCC 6803 in response to gradual transitions between nitrogen-limited and light-limited growth conditions were measured in continuous cultures. Transitions induced changes in pigment composition, light absorption coefficient, photosynthetic electron transport, and specific growth rate. Physiological changes were accompanied by reproducible changes in the expression of several hundred open reading frames, genes with functions in photosynthesis and respiration, carbon and nitrogen assimilation, protein synthesis, phosphorus metabolism, and overall regulation of cell function and proliferation. Cluster analysis of the nearly 1,600 regulated open reading frames identified eight clusters, each showing a different temporal response during the transitions. Two large clusters mirrored each other. One cluster included genes involved in photosynthesis, which were up-regulated during light-limited growth but down-regulated during nitrogen-limited growth. Conversely, genes in the other cluster were down-regulated during light-limited growth but up-regulated during nitrogen-limited growth; this cluster included several genes involved in nitrogen uptake and assimilation. These results demonstrate complementary regulation of gene expression for two major metabolic activities of cyanobacteria. Comparison with batch-culture experiments revealed interesting differences in gene expression between batch and continuous culture and illustrates that continuous-culture experiments can pick up subtle changes in cell physiology and gene expression.
authors.affiliation: Aquatic Microbiology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.
authors.forename: Eneas
authors.initials: E
authors.lastname: Aguirre von Wobeser
authors.forename: Bas W
authors.initials: BW
authors.lastname: Ibelings
authors.forename: Jasper
authors.initials: J
authors.lastname: Bok
authors.forename: Vladimir
authors.initials: V
authors.lastname: Krasikov
authors.forename: Jef
authors.initials: J
authors.lastname: Huisman
authors.forename: Hans C P
authors.initials: HC
authors.lastname: Matthijs
mesh_terms: D016000:Cluster Analysis; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000528:radiation effects*; D005798:Genes, Bacterial / Q000235:genetics; D008027:Light; D005810:Multigene Family / Q000235:genetics; D009584:Nitrogen / Q000494:pharmacology*; D013057:Spectrum Analysis; D046939:Synechocystis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*
chemical_list: D009584:Nitrogen