title: [Expressions of CD147 in peripheral monocytes and T lymphocytes of patients with ankylosing spondylitis].
abstract: OBJECTIVE
  To investigate the roles of CD147 in the pathogenesis and development of ankylosing spondylitis (AS).
  
  
  METHODS
  Flow cytometry was used to detect the expression levels of CD147 in peripheral monocytes and T lymphocytes of 30 AS patients, 30 rheumatoid arthritis (RA) patients and 30 healthy controls (HC). reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the expression levels of CD147 mRNA in peripheral blood mononuclear cells (PBMC). Then the expression levels of CD147 were compared among the groups. And a correlation analysis was conducted between CD147 levels and disease activity indices of AS patients.
  
  
  RESULTS
  The mean fluorescence intensities of CD147 in monocytes of AS, RA and HC group were 213.5 ± 37.8, 228.7 ± 49.7 and 163.6 ± 44.8, and in T lymphocytes 36.8 ± 10.1, 40.2 ± 10.5 and 28.3 ± 10.6 respectively. Both the expression levels of CD147 in monocytes and T lymphocytes of AS patients were slightly lower than those of RA patients. But the differences was not statistically significant (P > 0.05). Both the CD147 levels in monocytes and T lymphocytes of AS and RA group were significantly higher than those of HC group (P < 0.05). The expression levels of CD147 mRNA in PBMC of AS and RA group were significantly higher than those of HC group (P < 0.05) while no significant difference was found between AS and RA group (P > 0.05). Both the expression levels of CD147 in monocytes and T lymphocytes of AS patients were positively correlated with the patients' erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP).
  
  
  CONCLUSIONS
  The expressions of CD147 in peripheral monocytes and T lymphocytes of AS patients are up-regulated and their levels are positively correlated with patients' ESR and CRP. It implies that CD147 plays critical roles in the pathogenesis and development of AS.
authors.affiliation: Department of Rheumatology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Zhi-xiang
authors.initials: ZX
authors.lastname: Huang
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Pan
authors.forename: Fu-cheng
authors.initials: FC
authors.lastname: Zhang
authors.forename: Ben-rong
authors.initials: BR
authors.lastname: Zheng
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Deng
authors.forename: Tian-wang
authors.initials: TW
authors.lastname: Li
authors.forename: Jie-ruo
authors.initials: JR
authors.lastname: Gu
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood; D051926:Basigin / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D013167:Spondylitis, Ankylosing / Q000097:blood / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D051926:Basigin