title: Comparison of two Mycoplasma genitalium real-time PCR detection methodologies.
abstract: Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).
authors.affiliation: Department of Microbiology & Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia. jimmy.twin@mcri.edu.au
authors.forename: Jimmy
authors.initials: J
authors.lastname: Twin
authors.forename: Nicole
authors.initials: N
authors.lastname: Taylor
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Garland
authors.forename: Jane S
authors.initials: JS
authors.lastname: Hocking
authors.forename: Jennifer
authors.initials: J
authors.lastname: Walker
authors.forename: Catriona S
authors.initials: CS
authors.lastname: Bradshaw
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Fairley
authors.forename: Sepehr N
authors.initials: SN
authors.lastname: Tabrizi
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D045704:Mycoplasma genitalium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S