title: The CoREST/REST repressor is both necessary and inimical for expression of herpes simplex virus genes.
abstract: Herpes simplex virus type 1 encodes three sets of genes, α, β, and γ, whose expression is sequentially ordered in a cascade fashion. The transactivators of α genes comprise virion protein 16 (VP16) and the cellular proteins octamer binding protein 1 (Oct1) and host factor 1 (HCF1). Efficient transition from α to β gene expression requires the α protein ICP0 (infected cell protein 0). Earlier studies have shown that this protein binds to CoREST and displaces HDAC1 from the CoREST/REST/lysine-specific demethylase 1 (LSD1) repressor complex. Ultimately, the components of the repressor complex are translocated at least in part into the cytoplasm. A key event in activation of α genes is the recruitment of LSD1 to demethylate histones bound to the α gene promoters. LSD1 is unstable in the absence of its partner, CoREST, and raises the question of whether both CoREST and REST are involved in the initiation of transcription of the α genes. Here we show that CoREST or REST small interfering RNAs (siRNAs) destabilize CoREST, REST, LSD1, and Sin3A, another component of the repressor complex. In cells transfected with REST or CoREST siRNA, the accumulation of α proteins and mRNAs is delayed in comparison to those of mock-transfected or control siRNA-transfected cells. The LSD1/CoREST/REST compressor complex is thus sequentially necessary and subsequently inimical for viral gene expression.
authors.affiliation: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Guoying
authors.initials: G
authors.lastname: Zhou
authors.forename: Du
authors.initials: D
authors.lastname: Te
authors.forename: Bernard
authors.initials: B
authors.lastname: Roizman
mesh_terms: D045744:Cell Line, Tumor; D056970:Co-Repressor Proteins; D015967:Gene Expression Regulation, Viral; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D000099229:RE1-Silencing Transcription Factor
chemical_list: D056970:Co-Repressor Proteins; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D000099229:RE1-Silencing Transcription Factor; C121019:RCOR1 protein, human