title: [Effects of adipose-derived stem cells transplantation on the neuronal apoptosis and the expression of Bcl-2 and caspase-12 in the brain post focal cerebral ischemia in rats].
abstract: OBJECTIVE
  To investigate the effects of adipose-derived stem cells (ADSCs) transplantation on neuronal apoptosis in the brain after focal cerebral ischemia in rats.
  
  
  METHODS
  72 male adult Sprague-Dawley rats were randomly divided into 4 groups: Sham-operated group , Middle cerebral artery occlusion (MCAO) group, Vehicle group and ADSC-treated group (n=18). MCAO model was established with the modified Longa's method. One day after right MCAO, 30 μL of cell suspension containing 1×10(6); cells were injected into the lateral ventricle of ADSC-treated group and the same dose of PBS was given to the vehicle group. At 4 d, 7 d and 14 d after MCAO, the apoptosis of neuron was detected by terminal deoxynucleotidyl transferase-mediated DNA nick-end labeling (TUNEL) method and the expression of Bcl-2 and caspase-12 in ischemic region was detected by immunohistochemistry and RT-PCR.
  
  
  RESULTS
  TUNEL-positive cells in ischemic region of ADSC-treated group were less than that in MCAO group and Vehicle group at 4 d, 7 d and 14 d post MCAO (P<0.05). Compared with MCAO group and Vehicle group, the expression of Bcl-2 significantly up-regulated while caspase-12 expression significantly decreased in ADSC-treated group at any time point post MCAO (P<0.05).
  
  
  CONCLUSIONS
  The transplantation of ADSCs can reduce neuronal apoptosis of rats with cerebral ischemic injury partly by promoting the expression of Bcl-2 which participates in apoptotic signals after mitochondrial damage and inhibiting the expression of caspase-12 which mediates endoplasmic reticulum (ER) stress-induced apoptosis.
authors.affiliation: Department of Neurology, Union Hospital of Fujian Medical University, Fuzhou 350001, China.
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Lin
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Xiao
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Hou-wei
authors.initials: HW
authors.lastname: DU
authors.forename: Jie-hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Yi-xian
authors.initials: YX
authors.lastname: Zhang
authors.forename: De-shan
authors.initials: DS
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism / Q000601:surgery; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism / Q000601:surgery*; D053454:Caspase 12 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D020244:Infarction, Middle Cerebral Artery / Q000235:genetics; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D053454:Caspase 12