title: Histology and symplasmic tracer distribution during development of barley androgenic embryos.
abstract: The present study concerns three aspects of barley androgenesis: (1) the morphology and histology of the embryos during their development, (2) the time course of fluorescent symplasmic tracers' distribution, and (3) the correlation between symplasmic communication and cell differentiation. The results indicate that barley embryos, which are developing via an androgenic pathway, resemble their zygotic counterparts with respect to their developmental stages, morphology and histology. Analysis of the distribution of the symplasmic tracers, HPTS, and uncaged fluorescein indicates the symplasmic isolation of (1) the protodermis from the underlying cells of the late globular stage onwards, and (2) the embryonic organs at the mature stage of development.
authors.affiliation: Laboratory of Cell Biology, University of Silesia, ul. Jagiellońska 28, 40-032 Katowice, Poland. justyna.wrobel@us.edu.pl
authors.forename: Justyna
authors.initials: J
authors.lastname: Wrobel
authors.forename: Peter W
authors.initials: PW
authors.lastname: Barlow
authors.forename: Karolina
authors.initials: K
authors.lastname: Gorka
authors.forename: Danuta
authors.initials: D
authors.lastname: Nabialkowska
authors.forename: Ewa U
authors.initials: EU
authors.lastname: Kurczynska
mesh_terms: D000728:Androgens / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002478:Cells, Cultured; D035264:Flowers / Q000254:growth & development / Q000378:metabolism; D005452:Fluoresceins / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D001467:Hordeum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000728:Androgens; D005452:Fluoresceins; D005456:Fluorescent Dyes