title: Cell adhesive and growth behavior on electrospun nanofibrous scaffolds by designed multifunctional composites.
abstract: Nanostructured biocomposite scaffolds of poly(l-lactide) (PLLA) blended with collagen (coll) or hydroxyapatite (HA), or both for tissue engineering application, were fabricated by electrospinning. The electrospun scaffolds were characterized for the morphology, chemical and tensile properties by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), water contact angle (WCA), Fourier transform infrared (FTIR) measurement, and tensile testing. Electrospun biocomposite scaffolds of PLLA and collagen or (and) HA in the diameter range of 200-700 nm mimic the nanoscale structure of the extracellular matrix (ECM) with a well-interconnection pore network structure. The presence of collagen in the scaffolds increased their hydrophility, and enhanced cell attachment and proliferation, while HA improved the tensile properties of the scaffolds. The biocompatibility of the electrospun scaffolds and the viability of contacting cells were evaluated by 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) nuclear staining and by fluorescein diacetate (FDA) and propidium iodide (PI) double staining methods. The results support the conclusion that 293T cells grew well on composite scaffolds. Compared with pure PLLA scaffolds a greater density of viable cells was seen on the composites, especially the PLLA/HA/collagen scaffolds.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Cao
authors.forename: Yi-Pan
authors.initials: YP
authors.lastname: Wu
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Fu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Cong-Ju
authors.initials: CJ
authors.lastname: Li
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Gao
authors.forename: Zhong-Liang
authors.initials: ZL
authors.lastname: Chen
authors.forename: Xi-Zeng
authors.initials: XZ
authors.lastname: Feng
mesh_terms: D002448:Cell Adhesion; D003094:Collagen / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D011091:Polyesters; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite