title: Arbuscular mycorrhizal symbiosis elicits proteome responses opposite of P-starvation in SO4 grapevine rootstock upon root colonisation with two Glomus species.
abstract: Although plant biotisation with arbuscular mycorrhizal fungi (AMF) is a promising strategy for improving plant health, a better knowledge regarding the molecular mechanisms involved is required. In this context, we sought to analyse the root proteome of grapevine rootstock Selection Oppenheim 4 (SO4) upon colonisation with two AMF. As expected, AMF colonisation stimulates plant biomass. At the proteome level, changes in protein amounts due to AMF colonisation resulted in 39 differentially accumulated two-dimensional electrophoresis spots in AMF roots relative to control. Out of them, 25 were co-identified in SO4 roots upon colonisation by Glomus irregulare and Glomus mosseae supporting the existence of conserved plant responses to AM symbiosis in a woody perennial species. Among the 18 proteins whose amount was reduced in AMF-colonised roots were proteins involved in glycolysis, protein synthesis and fate, defence and cell rescue, ethylene biosynthesis and purine and pyrimidine salvage degradation. The six co-identified proteins whose amount was increased had functions in energy production, signalling, protein synthesis and fate including proteases. Altogether these data confirmed that a part of the accommodation program of AMF previously characterized in annual plants is maintained within roots of the SO4 rootstock cuttings. Nonetheless, particular responses also occurred involving proteins of carbon metabolism, development and root architecture, defence and cell rescue, anthocyanin biosynthesis and P remobilization, previously reported as induced upon P-starvation. This suggests the occurrence of P reprioritization upon AMF colonization in a woody perennial plant species with agronomical interest.
authors.affiliation: Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga 1346, Itacorubi, Caixa Postal 476, CEP 88034-001, Florianópolis, Santa Catarina, Brazil.
authors.forename: Gabriela Claudia
authors.initials: GC
authors.lastname: Cangahuala-Inocente
authors.affiliation: Embrapa-Centro de Pesquisa Agroflorestal do Amapá, Code Postal 10, CEP 68902-280, Macapá, Amapá, Brazil.
authors.forename: Maguida Fabiana
authors.initials: MF
authors.lastname: Da Silva
authors.affiliation: UMR INRA 1088, CNRS 5184, U. Bourgogne, PME, INRA, BP 86510, 21065, Dijon Cedex, France.
authors.forename: Jean-Martial
authors.initials: JM
authors.lastname: Johnson
authors.affiliation: Laboratoire de Biotechnologies des Champignons, Département de Biologie Végétale, Université Cheikh Anta Diop de Dakar, BP 5005, Dakar, Sénégal.
authors.forename: Anicet
authors.initials: A
authors.lastname: Manga
authors.affiliation: UMR INRA 1088, CNRS 5184, U. Bourgogne, PME, INRA, BP 86510, 21065, Dijon Cedex, France.
authors.forename: Diederik
authors.initials: D
authors.lastname: van Tuinen
authors.affiliation: PAPPSO, 91190, Gif sur Yvette, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Henry
authors.affiliation: Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga 1346, Itacorubi, Caixa Postal 476, CEP 88034-001, Florianópolis, Santa Catarina, Brazil. plovato@mbox1.ufsc.br.
authors.forename: Paulo Emílio
authors.initials: PE
authors.lastname: Lovato
authors.affiliation: UMR INRA 1088, CNRS 5184, U. Bourgogne, PME, INRA, BP 86510, 21065, Dijon Cedex, France.
authors.forename: Eliane
authors.initials: E
authors.lastname: Dumas-Gaudot
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D055137:Glomeromycota / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010710:Phosphates / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics / Q000382:microbiology / Q000502:physiology*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis; D027843:Vitis / Q000737:chemistry / Q000235:genetics / Q000382:microbiology / Q000502:physiology*
chemical_list: D010710:Phosphates; D010940:Plant Proteins; D020543:Proteome