title: Immobilization of molecularly imprinted polymer nanoparticles in electrospun poly(vinyl alcohol) nanofibers.
abstract: We describe the fabrication of polymer nanofibers with entrapped molecularly imprinted polymer (MIP) nanoparticles and study their possible use in a fluorescence-based biosensor application. The MIP was imprinted with the fluorescent amino acid derivative dansyl-L-phenylalanine. Poly(vinyl alcohol) was used as a support for MIP nanoparticles because it is water-soluble and can be spun into very thin fibers. The fibers were characterized by atomic force microscopy and optical microscopy, and fluorescence microscopy was used for the characterization of target binding to the MIP. The fibers show close to 100% recovery upon extraction and rebinding of the target molecule. The selectivity of the system has been demonstrated through competitive binding experiments with nonfluorescent analogues boc-L-phenylalanine and boc-D-phenylalanine.
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
authors.forename: Silvia
authors.initials: S
authors.lastname: Piperno
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tse Sum Bui
authors.forename: Karsten
authors.initials: K
authors.lastname: Haupt
authors.forename: Levi A
authors.initials: LA
authors.lastname: Gheber
mesh_terms: D004560:Electricity; D054802:Molecular Imprinting; D057139:Nanofibers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis* / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011142:Polyvinyl Alcohol