title: [Endotoxin-induced generation of peroxynitrite in bovine pulmonary arterial endothelial cells].
abstract: OBJECTIVE
  To explore the significance of bovine pulmonary arterial endothelial cell (BPAEC) to produce peroxynitrite (ONOO-).
  
  
  METHODS
  The contents of nitrotyrosine (NT), a marker of ONOO- generation in vivo,were quantitatively measured with flow cytometric and immunofluorescent technique in cultured BPAEC induced by lipopolysaccharide (LPS), a major component of bacterial endotoxin. In addition, effect of ONOO- on micromorphologic features of BPAEC was observed.
  
  
  RESULTS
  LPS caused significant increase of NT in BPAEC in a dose-dependent manner, which was in part reversed by aminoguanidine, a selective inhibitor of inducible nitric oxide synthase. Exposure of BPAEC to exogenous ONOO- resulted in the cellular retro-contraction with diminished cellular body and increased width of cellular junction.
  
  
  CONCLUSIONS
  LPS may induce the ONOO- formation of BPAEC. Endothelial cell-derived ONOO- may mediate the deleterious effect of LPS on BPAEC per se.
authors.affiliation: Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Gu
authors.forename: Y L
authors.initials: YL
authors.lastname: Ling
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000009:adverse effects*; D030421:Peroxynitrous Acid / Q000096:biosynthesis*; D011651:Pulmonary Artery / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D030421:Peroxynitrous Acid