title: Ozone therapy and hyperbaric oxygen treatment in lung injury in septic rats.
abstract: Various therapeutic protocols were used for the management of sepsis including hyperbaric oxygen (HBO) therapy. It has been shown that ozone therapy (OT) reduced inflammation in several entities and exhibits some similarity with HBO in regard to mechanisms of action. We designed a study to evaluate the efficacy of OT in an experimental rat model of sepsis to compare with HBO. Male Wistar rats were divided into sham, sepsis+cefepime, sepsis+cefepime+HBO, and sepsis+cefepime+OT groups. Sepsis was induced by an intraperitoneal injection of Escherichia coli; HBO was administered twice daily; OT was set as intraperitoneal injections once a day. The treatments were continued for 5 days after the induction of sepsis. At the end of experiment, the lung tissues and blood samples were harvested for biochemical and histological analysis. Myeloperoxidase activities and oxidative stress parameters, and serum proinflammatory cytokine levels, IL-1β and TNF-α, were found to be ameliorated by the adjuvant use of HBO and OT in the lung tissue when compared with the antibiotherapy only group. Histologic evaluation of the lung tissue samples confirmed the biochemical outcome. Our data presented that both HBO and OT reduced inflammation and injury in the septic rats' lungs; a greater benefit was obtained for OT. The current study demonstrated that the administration of OT as well as HBO as adjuvant therapy may support antibiotherapy in protecting the lung against septic injury. HBO and OT reduced tissue oxidative stress, regulated the systemic inflammatory response, and abated cellular infiltration to the lung demonstrated by findings of MPO activity and histopathologic examination. These findings indicated that OT tended to be more effective than HBO, in particular regarding serum IL-1β, lung GSH-Px and histologic outcome.
authors.affiliation: Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Yamanel
authors.forename: Umit
authors.initials: U
authors.lastname: Kaldirim
authors.forename: Yesim
authors.initials: Y
authors.lastname: Oztas
authors.forename: Omer
authors.initials: O
authors.lastname: Coskun
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Poyrazoglu
authors.forename: Murat
authors.initials: M
authors.lastname: Durusu
authors.forename: Tuncer
authors.initials: T
authors.lastname: Cayci
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ozturk
authors.forename: Seref
authors.initials: S
authors.lastname: Demirbas
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yasar
authors.forename: Orhan
authors.initials: O
authors.lastname: Cinar
authors.forename: Salim Kemal
authors.initials: SK
authors.lastname: Tuncer
authors.forename: Yusuf Emrah
authors.initials: YE
authors.lastname: Eyi
authors.forename: Bulent
authors.initials: B
authors.lastname: Uysal
authors.forename: Turgut
authors.initials: T
authors.lastname: Topal
authors.forename: Sukru
authors.initials: S
authors.lastname: Oter
authors.forename: Ahmet
authors.initials: A
authors.lastname: Korkmaz
mesh_terms: D000818:Animals; D005978:Glutathione / Q000097:blood; D006931:Hyperbaric Oxygenation; D053583:Interleukin-1beta / Q000097:blood; D008168:Lung / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000097:blood / Q000150:complications / Q000473:pathology / Q000628:therapy*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010083:Oxidants, Photochemical / Q000627:therapeutic use; D010126:Ozone / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000150:complications / Q000473:pathology / Q000628:therapy*; D013482:Superoxide Dismutase / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D053583:Interleukin-1beta; D010083:Oxidants, Photochemical; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D010126:Ozone; D013482:Superoxide Dismutase; D005978:Glutathione