title: [Construction of Bifidobacterium Infantis/CD tumor targeting gene therapy system].
abstract: BACKGROUND
  To construct the Bifidobacterium Infantis/CD tumor targeting gene therapy system.
  
  
  METHODS
  PCR expanded CD gene and pGEX-1LambdaT plasmid was transferred into E.Coli. JM109. with TSS solution. Then a dual restriction enzyme (EcoR I and BamH I) digestion was carried out to cut CD gene and pGEX-1LambdaT plasmid. Two segments (4.9 kb and 1.3 kb) were selected and extracted from the gel. T4 DNA Ligase was added to these two segments' mixture. Finally, reconstruction fragment was transferred into Bifidobacterium Infantis by electroporation. The plasmid was extracted from the positive clone selected and testified by an agarose eletrophoresis after enzymed.
  
  
  RESULTS
  A positive transferred Bifidobacterium Infantis with 6.2 kb long reconstruction plasmid fragment with CD gene was established which was in accordance with theoretical calculation.
  
  
  CONCLUSIONS
  The Bifidobacterium Infantis/CD tumor targeting gene therapy system could be successfully constructed.
authors.affiliation: Department of Pathophysiology, West China Center of Medical Sciences of Sichuan University, Chengdu, Sichuan 610041, P.R.China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Guo
authors.forename: Cheng
authors.initials: C
authors.lastname: Yi
authors.forename: Shuren
authors.initials: S
authors.lastname: Wang
authors.forename: Haoyi
authors.initials: H
authors.lastname: Wang