title: The effect of a novel dinuclear platinum complex with berenil and 2-picoline ligands on growth of human breast cancer cells.
abstract: Evaluation of the cytotoxicity of a novel dinuclear platinum(II) complex of formula Pt2(2-picoline)4(berenil)2 employing a MTT assay and inhibition of [3H]thymidine incorporation into DNA in both MDA-MB-231 and MCF-7 breast cancer cells demonstrated that the complex was more potent anti-proliferative agent than cisplatin. Data from the ethidium displacement assay indicated that the complex showed specificity for AT base pairs of DNA. Our study showed that Pt2(2-picoline)4(berenil), was a potent catalytic inhibitor of topoisomerase II in opposition to cisplatin.
authors.affiliation: Department of Medicinal Chemistry and Drug Technology, Medical University of Białystok, Kilińskiego 1, 15-089 Białystok, Poland. kbiel@umwb.edu.pl
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Bielawski
authors.forename: Anna
authors.initials: A
authors.lastname: Bielawska
authors.forename: Bozena
authors.initials: B
authors.lastname: Popławska
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Surazyński
authors.forename: Robert
authors.initials: R
authors.lastname: Czarnomysy
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D009944:Organoplatinum Compounds / Q000378:metabolism / Q000494:pharmacology*; D010847:Picolines / Q000378:metabolism / Q000494:pharmacology*; D059005:Topoisomerase II Inhibitors / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009944:Organoplatinum Compounds; D010847:Picolines; C555763:Pt2(2-picoline)4(berenil)2; D059005:Topoisomerase II Inhibitors; D002945:Cisplatin