title: Aberrant expression of TFF1, TFF2, and PDX1 and their diagnostic value in lobular endocervical glandular hyperplasia.
abstract: Lobular endocervical glandular hyperplasia (LEGH) is a distinct benign glandular lesion expressing gastric gland mucous cell-type mucin (N-acetylglucosaminα1 → 4galactose → R [GlcNAcα1 → 4Gal → R]). To investigate histogenesis and diagnostic markers of LEGH, we examined the immunohistochemical expression profile of gastric surface mucous cell (MUC5AC and TFF1), gastric gland mucous cell (MUC6, TFF2, and GlcNAcα1 → 4Gal → R), gastric pyloric epithelial cell (PDX1), and endocervical cell (keratan sulfate) markers in normal endocervix samples and benign glandular lesions (nabothian cysts, tunnel clusters, and LEGHs). MUC5AC and MUC6 were expressed in normal endocervical mucosa and benign glandular lesions. TFF1, TFF2, GlcNAcα1 → 4Gal → R, and PDX1 were expressed only in LEGH. Keratan sulfate was expressed in normal endocervical mucosa and benign glandular lesions. In LEGH, gastric surface mucous cell and gastric gland mucous cell differentiation were demonstrated, and transdifferentiation from endocervical mucosa into gastric pyloric mucosa was suggested. In addition to GlcNAcα1 → 4Gal → R, TFF1, TFF2, and PDX1 are additional useful markers for LEGH.
authors.affiliation: Dept of Biomedical Sciences, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ota
authors.forename: Oi
authors.initials: O
authors.lastname: Harada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Uehara
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hayama
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishii
mesh_terms: D000328:Adult; D000368:Aged; D002584:Cervix Uteri / Q000473:pathology*; D005260:Female; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D007632:Keratan Sulfate / Q000096:biosynthesis; D008875:Middle Aged; D055271:Mucin 5AC / Q000096:biosynthesis*; D055272:Mucin-6 / Q000096:biosynthesis*; D009092:Mucous Membrane / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011230:Precancerous Conditions / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D011708:Pylorus / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis*; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology
chemical_list: D018398:Homeodomain Proteins; C524678:MUC5AC protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055272:Mucin-6; D010455:Peptides; C051838:TFF1 protein, human; C000604007:TFF2 protein, human; D015534:Trans-Activators; D000071163:Trefoil Factor-1; D000071164:Trefoil Factor-2; D025521:Tumor Suppressor Proteins; C084010:pancreatic and duodenal homeobox 1 protein; D007632:Keratan Sulfate