title: Licochalcone A inhibits growth of gastric cancer cells by arresting cell cycle progression and inducing apoptosis.
abstract: The aim of this study was to determine the anticancer effects of seven licorice compounds in MKN-28, AGS, and MKN-45 gastric cancer cells and human gastric epithelium immortalized cells. We also explored the mechanism of action of licochalcone A (LCA), the most cytotoxic licorice compound, by analyzing its influence on cell cycle progression and apoptosis. The results indicated that LCA was the most cytotoxic licorice compound of those tested, and it inhibited gastric cancer cells growth in a dose-dependent manner, with an IC50 value of approximately 40μM. LCA affected gastric cancer cell viability by blocking cell cycle progression at the G2/M transition and inducing apoptosis. LCA treatment increased the expression of Rb and decreased the expression of cyclin A, cyclin B and MDM2 in MKN-28, AGS and MKN-45 cell lines. In addition, LCA-induced apoptosis by its effects on the expression of PARP, caspase-3, Bcl-2 and Bax. These data provide evidence that LCA has the potential to be used in the treatment of gastric cancer.
authors.affiliation: Department of Pathology, Fudan University, Shanghai Cancer Center, Shanghai 200032, China.
authors.forename: Xiu-ying
authors.initials: XY
authors.lastname: Xiao
authors.forename: Miao
authors.initials: M
authors.lastname: Hao
authors.forename: Xin-ying
authors.initials: XY
authors.lastname: Yang
authors.forename: Qian
authors.initials: Q
authors.lastname: Ba
authors.forename: Mian
authors.initials: M
authors.lastname: Li
authors.forename: Shu-juan
authors.initials: SJ
authors.lastname: Ni
authors.forename: Li-Sha
authors.initials: LS
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Du
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016195:G2 Phase / Q000187:drug effects; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; D047188:Chalcones; C070840:licochalcone A