title: NMR analysis of seven selections of vermentino grape berry: metabolites composition and development.
abstract: The goal of this work was to study via NMR the unaltered metabolic profile of Sardinian Vermentino grape berry. Seven selections of Vermentino were harvested from the same vineyard. Berries were stored and extracted following an unbiased extraction protocol. Extracts were analyzed to investigate variability in metabolites concentration as a function of the clone, the position of berries in the bunch or growing area within the vineyard. Quantitative NMR and statistical analysis (PCA, correlation analysis, Anova) of the experimental data point out that, among the investigated sources of variation, the position of the berries within the bunch mainly influences the metabolic profile of berries, while the metabolic profile does not seem to be significantly influenced by growing area and clone. Significant variability of the amino acids such as arginine, proline, and organic acids (malic and citric) characterizes the rapid rearrangements of the metabolic profile in response to environmental stimuli. Finally, an application is described on the analysis of metabolite variation throughout the physiological development of berries.
authors.affiliation: Porto Conte Ricerche Srl, S.P. 55 Porto Conte/Capo Caccia, 07041 Tramariglio-Alghero (SS), Italy.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Mulas
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Galaffu
authors.forename: Luca
authors.initials: L
authors.lastname: Pretti
authors.forename: Gianni
authors.initials: G
authors.lastname: Nieddu
authors.forename: Luca
authors.initials: L
authors.lastname: Mercenaro
authors.forename: Roberto
authors.initials: R
authors.lastname: Tonelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Anedda
mesh_terms: D000596:Amino Acids / Q000032:analysis; D019343:Citric Acid / Q000032:analysis; D004777:Environment; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008293:Malates / Q000032:analysis; D055442:Metabolome; D010936:Plant Extracts / Q000737:chemistry; D013045:Species Specificity; D027843:Vitis / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D008293:Malates; D010936:Plant Extracts; D019343:Citric Acid; C030298:malic acid