title: Standardization of biochemical profile of mesenchymal cell materials by probing the level of dehydrogenase activity.
abstract: It is demonstrated that the output optical signal of MTT test is directly proportional to the number of viable cells in the primary culture of mesenchymal cells (skin fibroblasts and mesenchymal stem cells from the bone marrow, placenta, and umbilical cord). The slope of the best curve in coordinates "cell number - optical signal" reflecting specific productivity of MTT-formazan characterizes mean dehydrogenase activity of cells and their physiological activity. It was found that in vitro dehydrogenase activity of primary cultures of mesenchymal cells increased during the first 3-5 passages and then tended to decrease. The variant of MTT method presented here can be used for standardization of cell materials.
authors.affiliation: Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia. vburunova@mail.ru
authors.forename: V V
authors.initials: VV
authors.lastname: Burunova
authors.forename: A M
authors.initials: AM
authors.lastname: Gisina
authors.forename: I V
authors.initials: IV
authors.lastname: Kholodenko
authors.forename: A Yu
authors.initials: AY
authors.lastname: Lupatov
authors.forename: O A
authors.initials: OA
authors.lastname: Shragina
authors.forename: K N
authors.initials: KN
authors.lastname: Yarygin
mesh_terms: D001854:Bone Marrow Cells / Q000502:physiology; D018929:Cell Culture Techniques / Q000592:standards*; D002478:Cells, Cultured; D005260:Female; D005562:Formazans / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology; D010088:Oxidoreductases / Q000378:metabolism*; D011247:Pregnancy; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D005562:Formazans; D013778:Tetrazolium Salts; D013844:Thiazoles; C079782:MTT formazan; D010088:Oxidoreductases; C022616:thiazolyl blue