title: [Study on the association between DNA double-strand break repair gene NBS1 polymorphisms and susceptibility on lung cancer].
abstract: OBJECTIVE
  To study the association between DNA double-strand break repair gene NBS1 (nijmegen breakage syndrome gene) polymorphisms and the susceptibility to lung cancer.
  
  
  METHODS
  A case-control study design was applied. PCR-RFLP was used to identify NBS1 polymorphisms among 575 lung cancer cases and 575 controls.
  
  
  RESULTS
  The frequencies of C/C, C/G and G/G genotypes at NBS1 rs1805794 site were 25.9%, 51.8%, 22.3% among controls compared to 20.5%, 52.3%, 27.1% among cases. There was significant difference between controls and cases (χ(2) = 6.38, P = 0.04). Individuals carrying C/G + G/G genotypes had an increased risk for lung cancer (OR = 1.46, 95%CI: 1.09 - 1.97) compared to the C/C genotype. The frequencies of G/G, G/C and C/C genotypes at NBS1 rs2735383 site were 37.9%, 47.0%, 15.1% among controls compared to 35.5%, 48.5%, 16.0% among cases, with no significant difference between the two groups (χ(2) = 0.75, P = 0.69). Individuals carrying Hap4-GC haplotype (OR = 1.70, 95%CI: 1.24 - 2.31) and Hap4/Hap2 dihaplotype (OR = 1.75, 95%CI: 1.11 - 2.76) had an increased risk on lung cancer. Joint associations of smoking and the NBS1 polymorphism with the risk of lung cancer were observed (P < 0.05).
  
  
  CONCLUSIONS
  The G/G genotype at NBS1 rs1805794 site and the Hap4-GC haplotype and Hap4/Hap2 di haplotype from rs1805794 and rs2735383 were both associated with lung cancer.
authors.affiliation: Department of Epidemiology, School of Public Health, Fujian Medical University, Fuzhou 350004, China; Wenzhou Center for Disease Control and Prevention.
authors.forename: Li-hui
authors.initials: LH
authors.lastname: Fan
authors.forename: Jun-lei
authors.initials: JL
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Cai
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D000368:Aged; D016022:Case-Control Studies; D018797:Cell Cycle Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D018797:Cell Cycle Proteins; C112156:NBN protein, human; D009687:Nuclear Proteins; D004247:DNA