title: Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase.
abstract: A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfides, showing moderate to good enantioselectivities in forming chiral sulfoxides.
authors.affiliation: Departamento de Química Orgánica e Inorgánica, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, C/Julián Clavería 8, 33006, Oviedo, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Rioz-Martínez
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kopacz
authors.forename: Gonzalo
authors.initials: G
authors.lastname: de Gonzalo
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Torres Pazmiño
authors.forename: Vicente
authors.initials: V
authors.lastname: Gotor
authors.forename: Marco W
authors.initials: MW
authors.lastname: Fraaije
mesh_terms: D055162:Biocatalysis; D004926:Escherichia coli / Q000201:enzymology*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)