title: Immunolocalization of TSOL18 and TSOL45-1A, the successful protective peptides against porcine cysticercosis, in Taenia solium oncospheres.
abstract: Taenia solium life cycle includes humans as definitive hosts and pigs as intermediate hosts. One of the measures to stop the life cycle of this parasite is by vaccination of pigs. In experiments performed in pigs with TSOL18 and TSOL45-1A, two recombinant T. solium proteins, 99.5% and 97.0% protection was induced, respectively. The purpose of this paper was to localize these antigens in all stages of the parasite (adult worms, oncospheres and cysticerci) by immunofluorescence, with the use of antibodies against TSOL18 and TSOL45-1A that were obtained from the pigs used in the vaccination experiment. Results show that TSOL18 and TSOL45-1A are expressed on the surface of T. solium oncospheres and not in tapeworms or cysticerci, indicating that they are stage-specific antigens. This, therefore, might explain the high level of protection these antigens induce against pig cysticercosis.
authors.affiliation: Hospital General Dr. Manuel Gea Gonzalez, SSA. Calzada de Tlalpan 4800, Col Seccion XVI, Tlalpan, Mexico 14080 DF, Mexico.
authors.forename: Joel
authors.initials: J
authors.lastname: Martinez-Ocaña
authors.forename: Mirza
authors.initials: M
authors.lastname: Romero-Valdovinos
authors.forename: Rina G
authors.initials: RG
authors.lastname: de Kaminsky
authors.forename: Pablo
authors.initials: P
authors.lastname: Maravilla
authors.forename: Ana
authors.initials: A
authors.lastname: Flisser
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology*; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D008856:Microscopy, Fluorescence; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control*; D041201:Taenia solium / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; C113407:TSOL-18 antigen