title: Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids.
abstract: Bordetella dermonecrotic toxin (DNT) affects the biological function of host cells by activating intracellular Rho GTPases. The toxin binds to unidentified receptor(s) via 54 N-terminal amino acids, undergoes intramolecular cleavage on the C-terminal side of Arg(44) by furin or furin-like protease, and eventually enters the cytoplasm where the Rho GTPases reside. The binding to the receptor(s) and intramolecular cleavage are essential for DNT to intoxicate cells, and the 54 amino-acid binding domain encompasses the cleavage site, however, it is unclear whether these two events are related. In this study, we could narrow down the cell-binding domain to the N-terminal amino acids 2-30. The region does not contain the furin-recognition site, indicating that the cell binding and the intramolecular cleavage are independent events.
authors.affiliation: Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Fukui-Miyazaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Ohnishi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kamitani
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Abe
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Horiguchi
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001884:Bordetella / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D011503:Transglutaminases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000596:Amino Acids; D007074:Immunoglobulin G; D010455:Peptides; D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; C060969:dermonecrotic toxin, Bordetella; D008156:Luciferases; D011503:Transglutaminases; D020741:rho GTP-Binding Proteins