title: Bioreduction of α,β-unsaturated ketones and aldehydes by non-conventional yeast (NCY) whole-cells.
abstract: The bioreduction of α,β-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone) and aldehydes [(S)-(-)-perillaldehyde and α-methyl-cinnamaldehyde] by 23 "non-conventional" yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated CC bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed.
authors.affiliation: Department of Applied Biology and Industrial Yeasts Collection DBVPG, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Goretti
authors.forename: Chiara
authors.initials: C
authors.lastname: Ponzoni
authors.forename: Elisa
authors.initials: E
authors.lastname: Caselli
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Marchegiani
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Cramarossa
authors.forename: Benedetta
authors.initials: B
authors.lastname: Turchetti
authors.forename: Luca
authors.initials: L
authors.lastname: Forti
authors.forename: Pietro
authors.initials: P
authors.lastname: Buzzini
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D055162:Biocatalysis; D001711:Biotransformation / Q000502:physiology; D003512:Cyclohexanones / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D007659:Ketones / Q000378:metabolism*; D039821:Monoterpenes / Q000378:metabolism; D010084:Oxidation-Reduction; D015003:Yeasts / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D003512:Cyclohexanones; D003517:Cyclopentanes; D007659:Ketones; D039821:Monoterpenes; C476259:ketoisophorone; C007201:cyclopentanone; C033342:perillaldehyde; D000171:Acrolein; C012843:cinnamaldehyde