title: Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing.
abstract: In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.
authors.affiliation: Department of Physics and Astronomy, University of California Los Angeles, Los Angeles, California, USA.
authors.forename: Adrian
authors.initials: A
authors.lastname: Cheng
authors.forename: J Tiago
authors.initials: JT
authors.lastname: Gonçalves
authors.forename: Peyman
authors.initials: P
authors.lastname: Golshani
authors.forename: Katsushi
authors.initials: K
authors.lastname: Arisaka
authors.forename: Carlos
authors.initials: C
authors.lastname: Portera-Cailliau
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D002118:Calcium