title: Conversion of oleic acid to 10-hydroxystearic acid by whole cells of Stenotrophomonas nitritireducens.
abstract: The optimal reaction conditions for the conversion of oleic acid to 10-hydroxystearic acid by whole cells of Stenotrophomonas nitritireducens were: pH 7.5, 35 °C, 0.05% (w/v) Tween 80, 20 g cells l(-1), and 30 g oleic acid l(-1) in an anaerobic atmosphere. Under these conditions, the cells produced 31.5 g 10-hydroxystearic acid l(-1) over 4 h with a conversion yield of 100% (mol/mol) and a productivity of 7.9 g l(-1) h(-1), indicating that oleic acid was converted completely to 10-hydroxystearic acid, with no detectable byproduct. This is the highest concentration, productivity, and yield of 10-hydroxystearic acid from oleic acid reported thus far.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Bi-Na
authors.initials: BN
authors.lastname: Kim
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Yeom
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D019301:Oleic Acid / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*; D020588:Stenotrophomonas / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D013229:Stearic Acids; D019301:Oleic Acid; C070376:10-hydroxystearic acid