title: Effect of Chinese herbal therapy on breast cancer adenocarcinoma cell lines.
abstract: Despite the widespread use of medicinal herbs to prevent and treat many diseases, including cancer, there are insufficient scientific data on the safety and efficacy of the majority of herbal therapies. The aim of this study was to assess the effect of a unique Chinese herbal therapy (CHT) from controlled manufactured concentrated powders, on an in vitro model of breast cancer. Three breast adenocarcinoma cell lines (MDA-231, MDA-453, T47D) were exposed to CHT for 72 h. Cell viability was assessed by XTT (sodium 3'-[1-(phenylaminocarbonyl)-3, 4-tetra zolium]-bis(4-methoxy-6-nitro) benzene sulphonic acid hydrate) assay. Apoptosis and cell cycle stage were determined by fluorescence-activated cell sorting (FACS) analysis. CHT decreased cell survival in a dose-dependent manner in all tested cell lines. FACS analysis of treated and non-treated T47D cells demonstrated that the inhibitory effect of CHT was associated with an increase in apoptosis. A randomized clinical trial is currently underway to investigate CHT as supplementary therapy for breast cancer patients receiving chemotherapy.
authors.affiliation: Unit of Integrative Medicine, Tel Aviv Sourasky Medical Centre, Tel Aviv, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Maimon
authors.forename: V
authors.initials: V
authors.lastname: Karaush
authors.forename: N
authors.initials: N
authors.lastname: Yaal-Hahoshen
authors.forename: R
authors.initials: R
authors.lastname: Ben-Yosef
authors.forename: I
authors.initials: I
authors.lastname: Ron
authors.forename: A
authors.initials: A
authors.lastname: Vexler
authors.forename: S
authors.initials: S
authors.lastname: Lev-Ari
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008517:Phytotherapy; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal