title: The effect of resveratrol on the Werner syndrome RecQ helicase gene and telomerase activity.
abstract: Various protein factors, including telomerase and WRN helicase, are involved in telomere maintenance. Resveratrol (Rsv), a polyphenol that extends the lifespan of diverse species is an activator of SIRT1, a NAD(+) dependent deacetylating enzyme in mammalian cells. Here, we examined the changes in gene expressions and promoter activities of WRN helicase and telomerase after Rsv treatment. This treatment increased the amount of WRN transcript and protein product by activating its promoter and telomerase promoter activity and gene expression. However cell proliferation was not changed. This suggests that Rsv induces telomere maintenance factors like WRN helicase without affecting cell proliferation.
authors.affiliation: Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. uchiumi@rs.noda.tus.ac.jp
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Uchiumi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Shin
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Taisuke
authors.initials: T
authors.lastname: Hoshi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Higami
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Tanuma
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology; D000071657:Werner Syndrome Helicase
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D019098:Telomerase; D005090:Exodeoxyribonucleases; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase; D000077185:Resveratrol