title: Arachidonate 12-lipoxygenase may serve as a potential marker and therapeutic target for prostate cancer stem cells.
abstract: The presence of arachidonate 12-lipoxygenase (12-LOX) potentiates prostate cancer (PCa) progression and therefore may be a good therapeutic target and/or a potential diagnostic predictor for PCa. In this study, we examined the expression of 12-LOX in PCa stem cells (PCa SCs) to test if it can serve as a unique marker and therapeutic target for PCa SCs. To this end, we isolated the cancer stem cell-like side population (SP) cells from the human PCa cell line DU-145 by a flow cytometry-based SP technique. The isolated DU-145 SP cells comprised a small population of the DU-145 cells. The SP cells had an up-regulation of ATP-binding cassette sub-family G member 2 (ABCG2) which enables these cells to efflux vital dyes and chemotherapeutic drugs. Furthermore, we detected a strong up-regulation of 12-LOX in these DU-145 SP cells compared to the parental DU-145 cells by RT-PCR and Western blot approaches. We also detected 12-LOX overexpression in PCa SCs in human PCa tissue samples by paraffin-section based immunofluorescent 4-channel confocal microscopy. However, no 12-LOX was detected in normal prostate epithelial SCs in normal prostate tissue samples. These multiple lines of evidence support the possibility that 12-LOX may serve as a unique marker and therapeutic target for PCa SCs.
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, PR China. yinbowzf@163.com
authors.forename: Bo
authors.initials: B
authors.lastname: Yin
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
authors.forename: Steven M
authors.initials: SM
authors.lastname: Mooney
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Xuewen
authors.initials: X
authors.lastname: Xu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Song
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology*; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D039421:Integrin alpha2 / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D039421:Integrin alpha2; D019012:Integrin beta1; D001092:Arachidonate 12-Lipoxygenase