title: Crystallization and preliminary X-ray analysis of a cold-active endo-β-1,4-D-xylanase from glycoside hydrolase family 8.
abstract: Endo-β-1,4-D-xylanases are used in a multitude of industrial applications. Native crystals of a cold-adapted xylanase from glycoside hydrolase family 8 were obtained by the vapour-diffusion technique. The crystals belonged to space group I222, with unit-cell parameters a=46.6, b=110.8, c=150.2 Å at 100 K, and diffracted to 2.7 Å resolution at a synchrotron source. The asymmetric unit is likely to contain one molecule, with a VM of 2.07 Å3 Da(-1), corresponding to a solvent content of ∼40%.
authors.affiliation: Laboratory for Biocrystallography, Department of Pharmaceutical Sciences, K. U. Leuven, Herestraat 49, O&N II, bus 822, 3000 Leuven, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Schoepe
authors.forename: Annick
authors.initials: A
authors.lastname: Pollet
authors.forename: Priscilla
authors.initials: P
authors.lastname: Verjans
authors.forename: Jan A
authors.initials: JA
authors.lastname: Delcour
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Strelkov
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Courtin
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003460:Crystallization; D018360:Crystallography, X-Ray; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D007527:Isoenzymes / Q000737:chemistry*; D008969:Molecular Sequence Data
chemical_list: D007527:Isoenzymes; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases