title: Expression of IMP1 enhances production of murine leukemia virus vector by facilitating viral genomic RNA packaging.
abstract: Murine leukemia virus (MLV)-based retroviral vector is widely used for gene transfer. Efficient packaging of the genomic RNA is critical for production of high-titer virus. Here, we report that expression of the insulin-like growth factor II mRNA binding protein 1 (IMP1) enhanced the production of infectious MLV vector. Overexpression of IMP1 increased the stability of viral genomic RNA in virus producer cells and packaging of the RNA into progeny virus in a dose-dependent manner. Downregulation of IMP1 in virus producer cells resulted in reduced production of the retroviral vector. These results indicate that IMP1 plays a role in regulating the packaging of MLV genomic RNA and can be used for improving production of retroviral vectors.
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Mai
authors.forename: Guangxia
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D001688:Biological Products / Q000235:genetics; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D012367:RNA, Viral / Q000235:genetics; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D012190:Retroviridae / Q000235:genetics; D019065:Virus Assembly / Q000235:genetics
chemical_list: D001688:Biological Products; C406144:IGF2BP1 protein, human; D012367:RNA, Viral; D016601:RNA-Binding Proteins; C105085:viron