title: RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs.
abstract: Cytoplasmic ribonucleoprotein granules, known as processing bodies (P-bodies), contain a common set of conserved RNA-processing enzymes, and mRNAs with AU-rich elements (AREs) are delivered to P-bodies for translational silencing. Although the dynamics of P-bodies is physically linked to cytoskeletal network, it is unclear how small GTPases are involved in the P-body regulation and the ARE-mRNA metabolism. We found here that glucose depletion activates RhoA GTPase and alters the P-body dynamics in HeLa cells. These glucose-depleted effects are reproduced by the overexpression of the RhoA-subfamily GTPases and conversely abolished by the inhibition of RhoA activation. Interestingly, both RhoA activation and glucose depletion inhibit the mRNA accumulation and degradation. These findings indicate that RhoA participates in the stress-induced rearrangement of P-bodies and the release of nucleated ARE-mRNAs for their stabilization.
authors.affiliation: Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Takahashi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sakurai
authors.forename: Arisa
authors.initials: A
authors.lastname: Ebihara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kajiho
authors.forename: Kota
authors.initials: K
authors.lastname: Saito
authors.forename: Kenji
authors.initials: K
authors.lastname: Kontani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishina
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Katada
mesh_terms: D000225:Adenine / Q000032:analysis; D000818:Animals; D003594:Cytoplasmic Granules / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000032:analysis; D051816:Tristetraprolin / Q000378:metabolism; D014498:Uracil / Q000032:analysis; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012261:Ribonucleoproteins; D051816:Tristetraprolin; D014498:Uracil; D020742:rhoA GTP-Binding Protein; D005947:Glucose; D000225:Adenine