title: Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues.
abstract: In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS(n) mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.
authors.affiliation: VTT Technical Research Centre of Finland, Espoo, Finland. heli.nygren@vtt.fi
authors.forename: Heli
authors.initials: H
authors.lastname: Nygren
authors.forename: Tuulikki
authors.initials: T
authors.lastname: Seppänen-Laakso
authors.forename: Sandra
authors.initials: S
authors.lastname: Castillo
authors.forename: Tuulia
authors.initials: T
authors.lastname: Hyötyläinen
authors.forename: Matej
authors.initials: M
authors.lastname: Orešič
mesh_terms: D053000:Analytic Sample Preparation Methods; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019963:Clinical Chemistry Tests / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D008055:Lipids