title: Structural analysis of N-glycans of the planarian Dugesia japonica.
abstract: To investigate the relationship between phylogeny and glycan structures, we analyzed the structure of planarian N-glycans. The planarian Dugesia japonica, a member of the flatworm family, is a lower metazoan. N-glycans were prepared from whole worms by hydrazinolysis, followed by tagging with the fluorophore 2-aminopyridine at their reducing end. The labeled N-glycans were purified, and separated by three HPLC steps. By comparison with standard pyridylaminated N-glycans, it was shown that the N-glycans of planarian include high mannose-type and pauci-mannose-type glycans. However, many of the major N-glycans from planarians have novel structures, as their elution positions did not match those of the standard glycans. The results of mass spectrometry and sugar component analyses indicated that these glycans include methyl mannoses, and that the most probable linkage was 3-O-methylation. Furthermore, the methyl residues on the most abundant glycan may be attached to the non-reducing-end mannose, as the glycans were resistant to α-mannosidase digestion. These results indicate that methylated high-mannose-type glycans are the most abundant structure in planarians.
authors.affiliation: Department of Biology, Faculty of Science, Niigata University, Japan. natsuka@bio.sc.niigata-u.ac.jp
authors.forename: Shunji
authors.initials: S
authors.lastname: Natsuka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hirohata
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nakakita
authors.forename: Wataru
authors.initials: W
authors.lastname: Sumiyoshi
authors.forename: Sumihiro
authors.initials: S
authors.lastname: Hase
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010932:Planarians / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides