title: Paclitaxel loaded nanosponges: in-vitro characterization and cytotoxicity study on MCF-7 cell line culture.
abstract: Beta cyclodextrin (β-CD) based nanosponges were synthesized and paclitaxel inclusion complex with nanosponges were prepared using techniques of inclusion complex formation. The paclitaxel nanosponge's complexes were evaluated for their release. The nanosponges complexes were also evaluated using DSC, FTIR, and NMR techniques for confirmation of inclusion complex formation between paclitaxel and nanosponges. Particle size and morphology of paclitaxel nanosponge's complex were estimated using SEM, TEM and dynamic light scattering techniques. The particle sizes were found out to be in range of 400 to 600 nm. Cytotoxic efficacy of paclitaxel nanosponge complex was determined against MCF-7 cells and paclitaxel nanosponge's complex was found to be cytotoxic and more effective against this cell line.
authors.affiliation: Centre for Novel Drug Delivery System, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai, India.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Ansari
authors.forename: Satyen J
authors.initials: SJ
authors.lastname: Torne
authors.forename: Pradeep R
authors.initials: PR
authors.lastname: Vavia
authors.forename: Francesco
authors.initials: F
authors.lastname: Trotta
authors.forename: Roberta
authors.initials: R
authors.lastname: Cavalli
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D004339:Drug Compounding; D005079:Excipients / Q000737:chemistry; D005260:Female; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009994:Osmolar Concentration; D017239:Paclitaxel / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D010316:Particle Size; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D005079:Excipients; D047392:beta-Cyclodextrins; C031215:betadex; D017239:Paclitaxel