title: Photopolymerized water-soluble chitosan-based hydrogel as potential use in tissue engineering.
abstract: Novel biodegradable hydrogels by photocrosslinking macromers based on chitosan derivative are reported. Photocrosslinkable macromers, a water-soluble (methacryloyloxy) ethyl carboxyethyl chitosan were prepared by Michael-addition reaction between chitosan and ethylene glycol acrylate methacrylate. The macromers were characterized by Fourier transform infrared spectroscopy, (1)H NMR and (13)C NMR. Hydrogels were fabricated by exposing aqueous solutions of macromers with 0.1% (w/v) photoinitiator to UV light irradiation, and their swelling kinetics as well as degradation behaviors was evaluated. The results demonstrated that the degradation rates were affected strongly by crosslinking density. The hydrogel was compatible to Vero cells, not exhibiting significant cytotoxicity. Cell culture assay also demonstrated that the hydrogels were good in promoting the cell attachment and proliferation, showing their potential as tissue engineering scaffolds.
authors.affiliation: Yunnan Baiyao Group Co., Ltd., 650118, PR China. zyssyz@126.com
authors.forename: Yingshan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guiping
authors.initials: G
authors.lastname: Ma
authors.forename: Suqing
authors.initials: S
authors.lastname: Shi
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Nie
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D002522:Chlorocebus aethiops; D004055:Differential Thermal Analysis; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008689:Methacrylates / Q000138:chemical synthesis; D051379:Mice; D012995:Solubility; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014466:Ultraviolet Rays; D014709:Vero Cells
chemical_list: D008689:Methacrylates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C005044:hydroxyethyl methacrylate; D048271:Chitosan