title: Polyploidization facilitates biotechnological in vitro techniques in the genus Cucumis.
abstract: Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7; muskmelon (C. melo) and wild Cucumis species, x = 12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.
authors.affiliation: Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, 783 71 Olomouc, Czech Republic. dagmar.skalova@upol.cz
authors.forename: Dagmar
authors.initials: D
authors.lastname: Skálová
authors.forename: Vladan
authors.initials: V
authors.lastname: Ondřej
authors.forename: Ivana
authors.initials: I
authors.lastname: Doležalová
authors.forename: Božena
authors.initials: B
authors.lastname: Navrátilová
authors.forename: Aleš
authors.initials: A
authors.lastname: Lebeda
mesh_terms: D001709:Biotechnology / Q000379:methods*; D058674:Chromosome Duplication; D028462:Cucumis / Q000235:genetics* / Q000502:physiology; D032621:DNA Shuffling / Q000379:methods; D006824:Hybridization, Genetic; D011003:Ploidies; D054817:Pollination / Q000502:physiology; D011123:Polyploidy; D013045:Species Specificity