title: Prolonged protein turnover of glyceraldehyde-3-phosphate dehydrogenase by phospholipase C-gamma 1 is critical for anchorage-independent growth and ATP synthesis in transformed cells.
abstract: Overexpression of phospholipase C-γl (PLC-γl) in rat 3Y1 fibroblasts leads to the formation of tumors in nude mice. However, the molecular mechanism for PLC-γl-mediated cellular transformation has not been studied in detail. In this study, we found that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme, protein levels were increased substantially in cells overexpressing PLC-γl, and that PLC-γl upregulation of GAPDH was due to a decrease in ubiquitination, followed by sustained protein turnover and subsequent accumulation. These observations suggest that regulation of the turnover rate of GAPDH is critical for anchorage-independent growth and ATP synthesis of transformed cells.
authors.affiliation: Department of Life Science, College of Natural Science, Daejin University, Pochen, Kyeonggido, Republic of Korea.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Yun
authors.forename: Sung-Kuk
authors.initials: SK
authors.lastname: Kim
authors.forename: Ho
authors.initials: H
authors.lastname: Kim
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D002471:Cell Transformation, Neoplastic; D003513:Cycloheximide / Q000494:pharmacology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006801:Humans; D016716:PC12 Cells; D051966:Phospholipase C gamma / Q000502:physiology*; D051381:Rats; D015398:Signal Transduction
chemical_list: D000255:Adenosine Triphosphate; D003513:Cycloheximide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D051966:Phospholipase C gamma