title: [The effect of nociception on expression of NADPH-d and fos protein at supraspinal level].
abstract: OBJECTIVE
  To study the distribution of NADPH-diaphorase positive neurons in periaqueductal gray matter(PAG) and dorsal raphe nucleus (DR), and the correlation between nitric oxide and peripheral nociception at supraspinal level.
  
  
  METHODS
  20 Sprague-Dawley rats were randomly divided into two groups. In experimental group (n = 10), formalin (5%, 0.1 ml) was injected subcutaneously into the plantar surface of the unilateral hindpaw, and in control group (n = 10), normal saline solution was used alike. The rats were perfused after 2 hours, and the midbrain were removed. Frozen seriate transverse sections were divided into three sets, all of which were processed for NADPH-d histochemistry. Subsequently, the first set that contained NADPH-diaphorase positive neurons were stained with neutral red, and the other two sets were processed for c-fos immunohistochemistry.
  
  
  RESULTS
  NADPH-diaphorase positive neurons were densely distributed in CGLD, CGLV and DR. NADPH-d, Fos and NADPH/Fos double-labeled neurons appeared in CGLD, CGLV and DR after injection of formalin in the rat hindpaw.
  
  
  CONCLUSIONS
  At supraspinal level NO may play an important role in the modulation of nociception in PAG and DR.
authors.affiliation: Xuzhou Medical College, Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou 221002, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Zhang
authors.forename: L C
authors.initials: LC
authors.lastname: Zhang
authors.forename: Y M
authors.initials: YM
authors.lastname: Zeng
authors.forename: J P
authors.initials: JP
authors.lastname: Cao
authors.forename: H J
authors.initials: HJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002535:Cerebral Aqueduct / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D059225:Nociception; D010146:Pain / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D011897:Random Allocation; D011903:Raphe Nuclei / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D009569:Nitric Oxide; D009249:NADP