title: [Nifedipine attenuates vascular inflammation via inhibin NF-κB activity].
abstract: OBJECTIVE
  To explore the effects and related mechanism of nifedipine on vascular inflammation induced by cuff placement.
  
  
  METHODS
  Adult male C57BL/6J mice (10 to 12 weeks of age) were assigned to control (no cuff placement without nifedipine), cuff placement (cuff placement without nifedipine) and treatment (cuff placement with nifedipine 1 or 5 mg×kg(-1)×d(-1)) groups. Activity of NF-κB in injured artery was measured 5 days after operation. MCP-1 expression and nuclear translocation of NF-κB were examined in injured artery 7 days after operation.
  
  
  RESULTS
  DNA-binding activity of NF-κB was significantly increased in the injured artery 5 days after cuff placement which could be downregulated by nifedipine 5 mg×kg(-1)×d(-1). MCP-1 mRNA expression in the injured arteries was increased 7 days after cuff placement and which could be significantly attenuated by nifedipine 5 mg×kg(-1)×d(-1). Cuff placement decreased the cytoplasmic level of p50, IκBα, IκBβ, and increased the nuclear level of p50. Nifedipine 5 mg×kg(-1)×d(-1) significantly attenuated these changes.
  
  
  CONCLUSIONS
  Our results suggest that high dose nifedipine could suppresses expression of MCP-1 induced by injured arteries via the inhibin NF-κB DNA binding activity, thereby attenuating vascular inflammation.
authors.affiliation: Department of Cardiology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, China. gaoxinyunm@hotmail.com
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Gao
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Shao-Kui
authors.initials: SK
authors.lastname: Liu
authors.forename: Fa-Qiang
authors.initials: FQ
authors.lastname: Lu
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Zhou
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000378:metabolism; D018932:Chemokine CCL2 / Q000378:metabolism; D007249:Inflammation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology*; D014652:Vascular Diseases / Q000378:metabolism*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D016328:NF-kappa B; D009543:Nifedipine