title: Prevention of follicular penetration: barrier-enhancing formulations against the penetration of pollen allergens into hair follicles.
abstract: The hair follicles could be a reservoir for topically applied substances. They are not only surrounded by a close network of blood capillaries, which makes them interesting for drug delivery, but they are also the host of dendritic cells, which are important for immunomodulation. Previously, pollen allergens were shown to penetrate into the hair follicles. The application of barrier-enhancing formulations might represent an effective strategy to prevent pollen protein penetration into the hair follicle. In the present study, porcine skin areas were pretreated with 4 barrier-enhancing emulsions. One skin area served as control and remained without pretreatment. Afterwards, fluorescein-isothiocyanate-labeled grass pollen proteins were applied to the porcine skin samples, and their penetration was investigated via fluorescent laser scanning microscopy. It was shown that the barrier-enhancing formulations were able to significantly reduce the penetration of exogenous proteins into the hair follicles, the extent of such reduction depending on the formulation.
authors.affiliation: Center of Experimental and Applied Cutaneous Physiology, Charité-Universitätsmedizin Berlin, Berlin, Germany. martina.meinke@charite.de
authors.forename: M C
authors.initials: MC
authors.lastname: Meinke
authors.forename: A
authors.initials: A
authors.lastname: Patzelt
authors.forename: H
authors.initials: H
authors.lastname: Richter
authors.forename: S
authors.initials: S
authors.lastname: Schanzer
authors.forename: W
authors.initials: W
authors.lastname: Sterry
authors.forename: A
authors.initials: A
authors.lastname: Filbry
authors.forename: K
authors.initials: K
authors.lastname: Bohnsack
authors.forename: F
authors.initials: F
authors.lastname: Rippke
authors.forename: J
authors.initials: J
authors.lastname: Galecka
authors.forename: R
authors.initials: R
authors.lastname: Fölster-Holst
authors.forename: J
authors.initials: J
authors.lastname: Lademann
mesh_terms: D000485:Allergens / Q000276:immunology / Q000493:pharmacokinetics*; D000818:Animals; D004655:Emulsions; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D018859:Hair Follicle / Q000378:metabolism*; D008055:Lipids / Q000008:administration & dosage / Q000494:pharmacology; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D012869:Skin Absorption / Q000187:drug effects*; D013552:Swine
chemical_list: D000485:Allergens; D004655:Emulsions; D005456:Fluorescent Dyes; D008055:Lipids; C039909:eucerin; D016650:Fluorescein-5-isothiocyanate