title: Vaccine for tuberculosis: up-regulation of IL-15 by Ag85A and not by ESAT-6.
abstract: IFN-γ is the most commonly measured cytokine released by the cells to define the cellular immune responses induced by the vaccine candidates for tuberculosis. IL-15 acts as a co-stimulator in IFN-γ production by NK cells and may therefore be important in the control of Mycobacterium tuberculosis that requires IFN-γ for clearance. The aim of the study is to determine whether Ag85A can also stimulate the innate immune response through the expression of IL-15, a cytokine that bridges the innate and adaptive immune systems. The expression of IL-15 was up regulated by about 4 fold in PPD+ healthy controls as compared with TB patients. Significantly higher expression of IL-15 mRNA in the Ag85A stimulated cells not only in PPD+ healthy controls but also in TB patients substantiates the use of Ag85A as a vaccine candidate over ESAT-6.
authors.affiliation: LEPRA-India, Blue Peter Public Health & Research Centre, Immunology and Molecular Biology Division, Cherlapally, Hyderabad 501301, AP, India. sudheerpydi@gmail.com
authors.forename: Satya Sudheer
authors.initials: SS
authors.lastname: Pydi
authors.forename: Anu Radha
authors.initials: AR
authors.lastname: Bandaru
authors.forename: Sambasivan
authors.initials: S
authors.lastname: Venkatasubramanian
authors.forename: Subbanna
authors.initials: S
authors.lastname: Jonnalagada
authors.forename: Vijaya Lakhsmi
authors.initials: VL
authors.lastname: Valluri
mesh_terms: D000217:Acyltransferases / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000096:biosynthesis; D019409:Interleukin-15 / Q000096:biosynthesis* / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D032581:Tuberculosis Vaccines / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; C525005:ESAT-6 antigen, Mycobacterium leprae; D019409:Interleukin-15; D012333:RNA, Messenger; D032581:Tuberculosis Vaccines; D007371:Interferon-gamma; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis