title: Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe.
abstract: We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.
authors.affiliation: Biomedical Research Center, Korea Institute of Science and Technology, 39-1 Haweolgog-Dong, Sungbook-Gu, Seoul 136-791, South Korea.
authors.forename: Seulki
authors.initials: S
authors.lastname: Lee
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Choi
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Chung
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Ryu
authors.forename: Aeju
authors.initials: A
authors.lastname: Lee
authors.forename: Heebeom
authors.initials: H
authors.lastname: Koo
authors.forename: In-Chan
authors.initials: IC
authors.lastname: Youn
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Park
authors.forename: In-San
authors.initials: IS
authors.lastname: Kim
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Kim
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Jeong
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Kwangmeyung
authors.initials: K
authors.lastname: Kim
authors.forename: Kuiwon
authors.initials: K
authors.lastname: Choi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements; D051379:Mice; D057054:Molecular Imaging; D015394:Molecular Structure; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009374:Neoplasms, Experimental; D010316:Particle Size; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D011108:Polymers; D004317:Doxorubicin; D053148:Caspase 3