title: Muskmelon embryo rescue techniques using in vitro embryo culture.
abstract: Among the major cucurbit vegetables, melon (Cucumis melo) has one of the greatest polymorphic fruit types and botanical varieties. Some melon fruits have excellent aroma, variety of flesh colors, deeper flavor, and more juice compared to other cucurbits. Despite numerous available melon cultivars, some of them are exceedingly susceptible to several diseases. The genetic background carrying the genes for tolerance and/or resistance for those diseases is found in wild melon landraces. Unfortunately, the commercial melon varieties are not able to produce viable hybrids when crossed with their wild melon counterparts. Plant tissue culture techniques are needed to surpass those genetic barriers. In vitro melon embryo rescue has played a main role to obtain viable hybrids originated from commercial versus wild melon crosses. In this chapter, an efficient and simple embryo rescue melon protocol is thoroughly described.
authors.affiliation: Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav)-Unidad Irapuato, Irapuato, Gto, Mexico.
authors.forename: Hector Gordon
authors.initials: HG
authors.lastname: Nuñez-Palenius
authors.forename: Rafael
authors.initials: R
authors.lastname: Ramírez-Malagón
authors.forename: Neftalí
authors.initials: N
authors.lastname: Ochoa-Alejo
mesh_terms: D028463:Cucumis melo / Q000196:embryology*; D003470:Culture Media; D046508:Culture Techniques; D005638:Fruit / Q000254:growth & development; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development*
chemical_list: D003470:Culture Media