title: The effects of acute exposure to deoxynivalenol on some inflammatory parameters in miniature pigs.
abstract: Seven miniature pigs were injected intravenously with deoxynivalenol (DON) at 1 mg/kg body weight; afterward, the number of leukocytes in peripheral blood, the luminol-dependent chemiluminescence of neutrophils, the serum or plasma concentration of cytokines and acute-phase proteins were evaluated to determine the effects of acute exposure to DON on inflammatory responses. White blood cell counts were transiently increased at 3, 6, and 12 hr post-injection (PI) due to the increased number of neutrophils. The luminol-dependent chemiluminescence value of neutrophils was significantly elevated at 24 hr PI, indicating the activation of the bactericidal function of neutrophils. Significant increases of interleukin (IL)-8 and tumor necrosis factor-α at 3 hr PI and IL-6 at 6 hr PI were detected in the serum. The concentration of haptoglobin and serum amyloid A was significantly increased at 24 hr PI. These results suggest that acute exposure to DON induced a temporary recruitment of neutrophils in the peripheral blood by IL-8 and subsequent activation of the bactericidal function, and a transient increase of proinflammatory cytokines and acute-phase proteins, indicating the immunomodulatory effects of DON in pigs.
authors.affiliation: Safety Research Team, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. mikami@affrc.go.jp
authors.forename: Osamu
authors.initials: O
authors.lastname: Mikami
authors.forename: Midori
authors.initials: M
authors.lastname: Kubo
authors.forename: Hideo
authors.initials: H
authors.lastname: Murata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muneta
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Tanimura
authors.forename: Ken
authors.initials: K
authors.lastname: Katsuda
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000662:veterinary*; D049449:Luminescence; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000139:chemically induced*; D013556:Swine, Miniature; D013997:Time Factors; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D000209:Acute-Phase Proteins; D016207:Cytokines; D014255:Trichothecenes; C007262:deoxynivalenol