title: [The use of dental papillae cells and millipore filter for bioengineered dentin].
abstract: OBJECTIVE
  To observe the ability of SD rat dental papillae cells forming dentin-like structure induced by millipore filter combined with transforming growth factor-β(1) (TGF-β(1)).
  
  
  METHODS
  The first passage SD rat dental papillae cells were enzymatically dissociated and centrifuged to obtain a cell mass. The cell mass was seeded on the millipore filter combined with TGF-β(1). The complex was incubated for 6 d in vitro or transplanted under the renal capsule for 2 weeks. Then the differentiation of dental papillae cells on the filter and the formation of mineral tissue on the implant were analyzed.
  
  
  RESULTS
  A layer of polarized columnar cells were observed along the surface of the millipore filter, with cell processes extending into the porous media. Dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP-1) were positive in these cells. After 2 weeks, tubular dentin matrix was deposited on the surface of the aligned cells. Scanning electron microscopy showed that the thickness of newly formed tubular dentin was consistent. DSP and DMP-1 were expressed in columnar cells, tubular matrix and the dental papillae cells adjacent to the filter.
  
  
  CONCLUSIONS
  The millipore filter combined with TGF-β(1) could effectively recruit progenitors onto its surface and induce odontoblast differentiation, secrete matrix in a homogenous manner, leading to dentinogenesis.
authors.affiliation: Department of Operative Dentistry & Endodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei-jia
authors.initials: WJ
authors.lastname: Ni
authors.forename: Yu-cheng
authors.initials: YC
authors.lastname: Li
authors.forename: Jia-yuan
authors.initials: JY
authors.lastname: Wu
authors.forename: Long-xing
authors.initials: LX
authors.lastname: Ni
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003771:Dental Papilla / Q000166:cytology*; D003804:Dentin; D003810:Dentinogenesis; D005109:Extracellular Matrix; D016326:Extracellular Matrix Proteins; D008851:Micropore Filters; D009804:Odontoblasts; D010750:Phosphoproteins; D051381:Rats; D012795:Sialoglycoproteins; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000099311:Dentin Sialophosphoprotein; C508960:TGFB1 protein, human