title: A CMP-N-acetylneuraminic acid synthetase purified from a marine bacterium, Photobacterium leiognathi JT-SHIZ-145.
abstract: A cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) synthetase was found in a crude extract prepared from Photobacterium leiognathi JT-SHIZ-145, a marine bacterium that also produces a β-galactoside α2,6-sialyltransferase. The CMP-Neu5Ac synthetase was purified from the crude extract of the cells by a combination of anion-exchange and gel filtration column chromatography. The purified enzyme migrated as a single band (60 kDa) on sodium dodecylsulfate-polyacrylamide gel electrophoresis. The activity of the enzyme was maximal at 35 °C at pH 9.0, and the synthetase required Mg(2+) for activity. Although these properties are similar to those of other CMP-Neu5Ac synthetases isolated from bacteria, this synthetase produced not only CMP-Neu5Ac from cytidine triphosphate and Neu5Ac, but also CMP-N-glycolylneuraminic acid from cytidine triphosphate and N-glycolylneuraminic acid, unlike CMP-Neu5Ac synthetase purified from Escherichia coli.
authors.affiliation: Glycotechnology Business Unit, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan. hitomi.kajiwara@jt.com
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kajiwara
authors.forename: Toshiki
authors.initials: T
authors.lastname: Mine
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D007700:Kinetics; D000216:N-Acylneuraminate Cytidylyltransferase / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D010776:Photobacterium / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism; D013379:Substrate Specificity; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D009844:Oligosaccharides; C016735:sialooligosaccharides; D012799:Sialyltransferases; D000216:N-Acylneuraminate Cytidylyltransferase; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase