title: The use of two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF-MS) for metabolomic analysis of polar metabolites.
abstract: Metabolites produced by an organism can be quite extensive, and one analytical technique alone is not capable of their comprehensive detection and identification. The majority of environmental metabolomic studies have implemented proton nuclear magnetic resonance ((1)H-NMR) spectroscopy with little attention given to mass spectrometry (MS) techniques. In this chapter, an analytical technique is outlined that incorporates two-dimensional gas chromatography-time-of-flight MS (GC×GC-TOF-MS) for the identification and quantification of polar metabolites.
authors.affiliation: Ecosystem Research Division, National Research Council Post-Doctoral Fellow, United States Environmental Protection Agency, Athens, GA, USA. Ralston-Hooper.Kimberly@epa.gov
authors.forename: Kimberly
authors.initials: K
authors.lastname: Ralston-Hooper
authors.forename: Amber
authors.initials: A
authors.lastname: Jannasch
authors.forename: Jiri
authors.initials: J
authors.lastname: Adamec
authors.forename: Maria
authors.initials: M
authors.lastname: Sepúlveda
mesh_terms: D053000:Analytic Sample Preparation Methods; D002849:Chromatography, Gas / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D013223:Statistics as Topic; D013997:Time Factors