title: Identification of the elusive peptidergic diuretic hormone in the blood-feeding bug Rhodnius prolixus: a CRF-related peptide.
abstract: Probing of a host and ingestion of a blood-meal in a fifth instar Rhodnius prolixus results in a cascade of tightly integrated events. The huge blood-meal is pumped into the anterior midgut during feeding, then modified by diuresis and stored until it is digested. While serotonin is known to be a diuretic hormone in R. prolixus, a peptidergic factor(s) was also known to play a role in diuresis. In the present study we employed molecular techniques and mass spectrometry to determine the sequence of a native CRF-like peptide from R. prolixus (Rhopr DH). In addition, we confirmed the distribution and localization of Rhopr DH using in situ hybridization and immunohistochemistry, and demonstrated its potent biological activity on both the anterior midgut and Malpighian tubules.
authors.affiliation: Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON, Canada, L5L 1C6. victoria.tebrugge@utoronto.ca
authors.forename: Victoria
authors.initials: V
authors.lastname: Te Brugge
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Paluzzi
authors.forename: David A
authors.initials: DA
authors.lastname: Schooley
authors.forename: Ian
authors.initials: I
authors.lastname: Orchard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D056148:Chromatography, Reverse-Phase; D003346:Corticotropin-Releasing Hormone / Q000737:chemistry*; D004064:Digestive System / Q000737:chemistry; D004231:Diuresis / Q000502:physiology; D005247:Feeding Behavior; D005455:Fluorescent Antibody Technique; D017403:In Situ Hybridization; D007301:Insect Hormones / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D019476:Insect Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008317:Malpighian Tubules / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012239:Rhodnius / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D007301:Insect Hormones; D019476:Insect Proteins; D003346:Corticotropin-Releasing Hormone