title: Kallikrein 1 is overexpressed by astrocytes in the hippocampus of patients with refractory temporal lobe epilepsy, associated with hippocampal sclerosis.
abstract: Kallikrein 1 (hK1) is a tissue enzyme responsible for kinin release in inflammatory cascade. This study was delineated to study the distribution and the co-localization of hK1 and kinin B1 and B2 receptors with glial and/or neuronal proteins markers, in the hippocampus of patients with refractory temporal lobe epilepsy, associated with hippocampal sclerosis (TLE-HS), comparing with control tissues. Hippocampal levels of KLK1 mRNA were also measured. hK1, kinin B1 and B2 receptors, NeuN and GFAP were analyzed using immunohistochemistry and confocal microscopy and KLK1 mRNA was quantified with real time PCR. Increased expression of hK1 by astrocytes co-localized with GFAP was found, contrasting with kinin B1 and B2 receptors, which were co-localized with NeuN in the sclerotic hippocampus. In addition, KLK1 mRNA was also up-regulated in same tissues. These data suggest an overexpression of kallikrein-kinin system and a neuron-glia interaction in the inflammatory process present in refractory TLE-HS.
authors.affiliation: Neurology and Neurosurgery Department, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
authors.forename: Priscila Santos Rodrigues
authors.initials: PS
authors.lastname: Simões
authors.forename: Sandra Regina
authors.initials: SR
authors.lastname: Perosa
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Arganãraz
authors.forename: Elza Márcia
authors.initials: EM
authors.lastname: Yacubian
authors.forename: Henrique
authors.initials: H
authors.lastname: Carrete
authors.forename: Ricardo Silva
authors.initials: RS
authors.lastname: Centeno
authors.forename: Pedro Paulo Vasconcellos
authors.initials: PP
authors.lastname: Varella
authors.forename: Joselita Ferreira Carvalho
authors.initials: JF
authors.lastname: Santiago
authors.forename: Mauro
authors.initials: M
authors.lastname: Canzian
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Silva
authors.forename: Renato Arruda
authors.initials: RA
authors.lastname: Mortara
authors.forename: Débora
authors.initials: D
authors.lastname: Amado
authors.forename: Esper Abrão
authors.initials: EA
authors.lastname: Cavalheiro
authors.forename: Maria da Graça Naffah
authors.initials: Mda G
authors.lastname: Mazzacoratti
mesh_terms: D000328:Adult; D001253:Astrocytes / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D004833:Epilepsy, Temporal Lobe / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012598:Sclerosis / Q000150:complications / Q000378:metabolism*; D020840:Tissue Kallikreins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D020840:Tissue Kallikreins