title: Influence of tetracycline resistance on the transport of manure-derived Escherichia coli in saturated porous media.
abstract: In this research, tetracycline resistant (tet(R)) and tetracycline susceptible (tet(S)) Escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of E. coli in saturated porous media was investigated through laboratory column transport experiments. Experimental results showed that tet(R)E. coli strains had higher mobility than the tet(S) strains in saturated porous media. Measurements of cell surface properties suggested that tet(R)E. coli strains exhibited lower zeta potentials than the tet(S) strains. Because the surface of clean quartz sands is negatively charged, the repulsive electrostatic double layer (EDL) interaction between the tet(R) cells and the surface of sands was stronger and thus facilitated the transport of the tet(R) cells. Although no difference was observed in surface acidity, cell size, lipopolysaccharides (LPS) sugar content and cell-bound protein levels between the tet(R) and tet(S) strains, they displayed distinct outer membrane protein (OMP) profiles. It was likely that the difference in OMPs, some potentially related to drug efflux pumps, between the tet(R) and tet(S) strains led to alteration in cell surface properties which in turn affected cell transport in saturated porous media. Findings from this research suggested that manure-derived tet(R)E. coli could spread more widely in the groundwater system and pose serious public health risks.
authors.affiliation: Department of Geosciences, 3209 N Maryland Ave, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
authors.forename: Jacob J
authors.initials: JJ
authors.lastname: Walczak
authors.forename: Sonia L
authors.initials: SL
authors.lastname: Bardy
authors.forename: Lucia
authors.initials: L
authors.lastname: Feriancikova
authors.forename: Shangping
authors.initials: S
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D004777:Environment; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D008372:Manure / Q000382:microbiology*; D009068:Movement / Q000187:drug effects; D009994:Osmolar Concentration; D016133:Polymerase Chain Reaction; D016062:Porosity; D012988:Soil Microbiology; D055672:Static Electricity; D013499:Surface Properties / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D008372:Manure; D013752:Tetracycline