title: Identification and characterization of myeloma-associated antigens in Trichinella spiralis.
abstract: To investigate the presence of myeloma-associated antigens in Trichinella spiralis and their anti-tumor effect, cross-immune responses between antigens of the myeloma cell SP2/0 versus positive sera to T. spiralis, and antigens of T. spiralis versus positive sera to myeloma cell SP2/0 were determined using T. spiralis and myeloma specific enzyme-linked immunosorbent assays (ELISA). The myeloma-associated antigens in T. spiralis were separated by ultrafiltration and 2-D electrophoresis, and the amino acid sequences and molecular weights were determined by spectrometry. An obvious reaction was found between a 33 kDa antigen and positive sera, and the major component of the antigen was tropomyosin (TM), which is an surface acidic protein with 284 amino acids. Mice were immunized with TM to determine the anti-tumor effect in vivo. The results showed that CD4(+), CD8(+) T lymphocyte, and CD19(+) B lymphocyte were significantly increased (P<0.05). The anti-tumor effects were significantly different between mice immunized with the antigens or adjuvant alone (P<0.05), while the difference between mice immunized with antigens and whole T. spiralis was not significant (P>0.05). The results indicated that TM identified in this study may play a role in eliciting cross-protective immunity.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Jingzhi
authors.initials: J
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Cao
authors.forename: Zhang
authors.initials: Z
authors.lastname: Nan
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.forename: Hengtong
authors.initials: H
authors.lastname: Fang
authors.forename: Huping
authors.initials: H
authors.lastname: Jiao
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Libo
authors.initials: L
authors.lastname: Su
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000276:immunology*; D009194:Myeloma Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D011897:Random Allocation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017160:Trichinella spiralis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D000951:Antigens, Neoplasm; D009194:Myeloma Proteins