title: Comparative evaluation of oxidative enzyme activities during adventitious rooting in the cuttings of grapevine rootstocks.
abstract: BACKGROUND
  This study investigated changes in peroxidase (POX) and polyphenol oxidase (PPO) activities through adventitious rooting in hardwood cuttings of grapevine rootstocks. Three grapevine rootstocks with different propensity to produce adventitious roots were selected: recalcitrant (Ramsey), non-recalcitrant (Rupestris du Lot) and intermediate (99R) cultivars.
  
  
  RESULTS
  The averages of root number at 65 days were 96 in Lot, 76 in 99R and 30 in Ramsey. Both enzyme activities characteristically increased before adventitious rooting, regardless of rooting ability of the rootstocks, and then decreased. POX activity increased in Ramsey cuttings at 22 days, in Lot and 99R cuttings at 14 days after planting, and then decreased gradually until 51 days. The highest POX activity was determined in Ramsey rootstock with the highest rooting ability and the lowest activity was determined in the rootstocks with the lowest rooting ability. PPO activity gradually increased in Ramsey rootstock cuttings from 10 days to 22 days, in Lot and 99R cuttings at 14 days, and then decreased until 51 days. A significant correlation was identified between high POX activity and adventitious rooting capability in rootstocks, but the same result was not determined with PPO activity.
  
  
  CONCLUSIONS
  A recalcitrant rooting variety cannot increase POX activity sufficiently before rooting. Therefore applications that could increase POX activity in stem cuttings during rooting may facilitate increased rooting in such rootstocks.
authors.affiliation: Department of Horticulture, Faculty of Agriculture, Atatürk University, 25240 Erzurum, Turkey.
authors.forename: Cafer
authors.initials: C
authors.lastname: Kose
authors.forename: Serkan
authors.initials: S
authors.lastname: Erdal
authors.forename: Ozkan
authors.initials: O
authors.lastname: Kaya
authors.forename: Okkeş
authors.initials: O
authors.lastname: Atici
mesh_terms: D000383:Agriculture / Q000379:methods*; D004156:Catechol Oxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology* / Q000254:growth & development; D013045:Species Specificity; D027843:Vitis / Q000145:classification / Q000201:enzymology* / Q000254:growth & development
chemical_list: D004156:Catechol Oxidase; D010544:Peroxidases