title: In vitro and in vivo inhibition of glucocorticoid-induced osteoporosis by the hexane extract of Poncirus trifoliata.
abstract: This study was performed to discover a novel herbal therapeutic for effective glucocorticoid-induced osteoporosis (GIO) treatment and further to clarify its molecular mechanism of action. Ethanol or methanol extracts of 68 edible Korean native plants were screened to find effective natural plant sources for the treatment of GIO, and Poncirus trifoliata (L.) (Rutaceae, PT) was selected as a final candidate because of its high inhibitory activity plus its novelty. The hexane extract of PT (PT-H) inhibited apoptotic cell death in dexamethasone-induced osteoblastic cell lines, C3H10T1/2 and MC3T3-E1. In vivo mouse results indicated that PT-H not only had an inhibitory effect on the bone loss caused by glucocorticoid, but also promoted bone formation. The molecular mechanisms behind the effect of PT-H on GIO were further clarified by screening of differentially expressed genes (DEGs) between dexamethasone (Dex)-induced osteoblastic cells with or without PT-H treatment. Finally, it was found that the expression level of AnxA6 in Dex-induced osteoblastic cells and prednisolone (PD)-treated GIO-model mice was significantly decreased by PT-H treatment. These findings suggest that PT-H has a strong in vitro and in vivo inhibitory effect on GIO, and decreased expression of AnxA6 may play a key role in this inhibition.
authors.affiliation: Departments of Medical Genetics, School of Medicine, Ajou University, Suwon 443-721, Republic of Korea.
authors.forename: Bo-Young
authors.initials: BY
authors.lastname: Kim
authors.forename: Hyung-Young
authors.initials: HY
authors.lastname: Yoon
authors.forename: Sun-Il
authors.initials: SI
authors.lastname: Yun
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.forename: Nan-Kyu
authors.initials: NK
authors.lastname: Song
authors.forename: Hyun-Gyu
authors.initials: HG
authors.lastname: Kim
authors.forename: Seon-Yong
authors.initials: SY
authors.lastname: Jeong
authors.forename: Yoon-Sok
authors.initials: YS
authors.lastname: Chung
mesh_terms: D000818:Animals; D017317:Annexin A6 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D003907:Dexamethasone / Q000009:adverse effects; D005938:Glucocorticoids / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010024:Osteoporosis / Q000139:chemically induced / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D032104:Poncirus / Q000737:chemistry*
chemical_list: D017317:Annexin A6; D005938:Glucocorticoids; D010936:Plant Extracts; D003907:Dexamethasone