title: Anti-inflammatory profile of FTO gene expression in adipose tissues from morbidly obese women.
abstract: BACKGROUND
  The fat mass and obesity associated (FTO) gene has been found to contribute to the risk of obesity in humans, but the function and regulation of FTO mRNA expression in adipose tissues remain to be clarified. Our aims were to assess the FTO gene expression in subcutaneous and visceral adipose tissues from morbidly obese women and its relation with obesity, insulin resistance indices, and most importantly, to obesity-related inflammatory markers.
  
  
  METHODS
  Paired subcutaneous and visceral fat were excised from 33 morbidly obese women and 12 control women who underwent bariatric surgery by laparoscopic gastric by-pass and elective surgery respectively. Adipose tissue mRNA expression was determined by real time RT-PCR.
  
  
  RESULTS
  FTO mRNA expression in visceral adipose tissue (VAT) was significantly higher than in subcutaneous adipose tissue (SAT) from obese but not control patients. SAT FTO expression was reduced in obese women compared to control subjects. It correlated negatively with BMI and insulin resistance indices. FTO expression in SAT was positively related to both circulating and mRNA levels of adiponectin, to adiponectin receptor and to PPAR-δexpression, but negatively with IL-6 gene expression and with circulating levels of leptin. FTO in VAT was also positively correlated with adiponectin, adiponectin receptor and PPAR-δ mRNA expression.
  
  
  CONCLUSIONS
  FTO expression in subcutaneous adipose tissue negatively correlates with obesity and insulin resistance. On the other hand, FTO presents a positive association with the expression of adiponectin, an anti-inflammatory adipokine, and with PPAR-δ in both adipose tissues. Taken together, our results suggest that FTO is associated with an anti-inflammatory behaviour in morbid obesity.
authors.affiliation: CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III (ISCIII), Madrid, Spain. ximena.terra@urv.cat
authors.forename: Ximena
authors.initials: X
authors.lastname: Terra
authors.forename: Teresa
authors.initials: T
authors.lastname: Auguet
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Porras
authors.forename: Yunuen
authors.initials: Y
authors.lastname: Quintero
authors.forename: Carmen
authors.initials: C
authors.lastname: Aguilar
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Luna
authors.forename: Mercé
authors.initials: M
authors.lastname: Hernández
authors.forename: Fátima
authors.initials: F
authors.lastname: Sabench
authors.forename: Daniel
authors.initials: D
authors.lastname: del Castillo
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Richart
mesh_terms: D054392:Adipokines / Q000235:genetics / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D015992:Body Mass Index; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D009767:Obesity, Morbid / Q000235:genetics / Q000378:metabolism*; D047494:PPAR delta / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D054392:Adipokines; D052242:Adiponectin; D015850:Interleukin-6; D020738:Leptin; D047494:PPAR delta; D011506:Proteins; D054419:Receptors, Adiponectin; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human