title: [The Effects of IFN-γ on AKT activated 32D Cells and its Mechanisms].
abstract: OBJECTIVE
  To investigate the effects of activated AKT on murine myeloid precursor cells (32D cells), and the effects of IFN-γ on 32D cells and its mechanisms.
  
  
  METHODS
  Plasmid transduction was used to enhance the expression of AKT on 32D cells. After the transfected cells treated with IFN-γ for 24 hours, proliferation rate was tested by WST-1, apoptosis by flow cytometry, expression of phosphorylated Erk1/2, Stat3 and phosphorylated Stat3 was determined by Western blot.
  
  
  RESULTS
  (1) IFN-γ at low concentration (100 U/ml) enhanced the growth and proliferation of 32D cells, while at high concentration (1000 U/ml) suppressed them. (2) Compared with control groups, low concentration IFN-γ increased (1124 ± 13) Stat3 phosphorylation in 32D-cell, while it high concentration IFN-γ decreased (601 ± 13). 32D cells transfected with activated Akt grew rapidly (0.287 ± 0.010) and had a low apoptotic rate [(9.57 ± 0.17)% (P < 0.05)]. (3) The expression of p-Erk1/2 in transfected 32D-cell was significantly reduced (P < 0.05). (4) Apoptosis rate of IFN-γ treated group was significantly decreased in transfected 32D cells (P < 0.05).
  
  
  CONCLUSIONS
  IFN-γ has dual effects on 32D cells, namely, at low concentration enhanced the growth and proliferation of 32D cells, while at high concentration suppressed them. Its mechanisims is possibly through Stat3 pathway. Activated Akt can significantly promote the growth and proliferation of 32D cell and significantly inhibit apoptosis and IFN-γ can regulate cell proliferation and apoptosis through AKT. AKT activation can inhibit the Erk signal pathway, which may be affected by inhibition the modificaton of Raf1.
authors.affiliation: Department of Hematology, Affiliated Hospital of Nantong University, Nantong 226001, China.
authors.forename: Lai-Gen
authors.initials: LG
authors.lastname: Tong
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Zeng-Hua
authors.initials: ZH
authors.lastname: Lin
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Li
authors.forename: Sheng-Hua
authors.initials: SH
authors.lastname: Jiang
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Chen
authors.forename: Hong-Ming
authors.initials: HM
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D050796:STAT3 Transcription Factor