title: Enhanced production of aureofuscin by over-expression of AURJ3M, positive regulator of aureofuscin biosynthesis in Streptomyces aureofuscus.
abstract: OBJECTIVE
  The production of aureofuscin is very low in the wild-type strain. We attempt to increase the production of aureofuscin by over-expression of a controlling gene in the wild-type strain.
  
  
  RESULTS
  The aurj3M gene was PCR-amplified from Streptomyces aureofuscus SYAU0709, ligated into vector pMD19 and sequenced. The predicted translation of the 579-bp cloned fragment was 97% similar to pimM from Streptomyces natalensis, which has an N-terminal PAS domain and a LuxR-type C-terminal helix-turn-helix. Recombinant bacterial strains were constructed by transforming SYAU0709 with an expression plasmid (pBJJ3M) that contained aurj3M, thereby increasing the number of aurj3M gene copies.
  
  
  CONCLUSIONS
  Bioassays for the antibiotic compound aureofuscin indicated that the recombinant bacteria had greater antifungal activity than the wild-type strain. Specifically, the recombinant strain produced approx. 600% more aureofuscin, as quantified by high-performance liquid chromatography analysis.
  
  
  CONCLUSIONS
  To our knowledge, this approach has not been attempted in S. aureofuscus before and few genes in the aureofuscin pathway have been cloned and characterized.
authors.affiliation: School of Life Science, Liaoning University, Shenyang, China  Food Academy, Shenyang Agricultural University, Shenyang, China. weij41177@yahoo.com.cn
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: X
authors.initials: X
authors.lastname: Meng
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019077:Helix-Turn-Helix Motifs; D008969:Molecular Sequence Data; D011090:Polyenes / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D011090:Polyenes; C048122:aureofuscin