title: Investigation of the apoptotic effect of curcumin in human leukemia HL-60 cells by using flow cytometry.
abstract: Curcumin (diferuloylmethane), the major yellow pigment isolated from the turmeric (Curcuma longa), has received much attention due to several biological properties. Curcumin exhibits a variety of pharmacological effects including antitumor, anti-inflammatory, and anti-infectious activities. In the present study, the effects of curcumin on apoptosis in the acute promyelocytic human leukemia (HL-60) cells was evaluated. Cytotoxic effects of curcumin on HL-60 cells were determined by MTT. HL-60 cells underwent apoptosis on treatment with curcumin, as indicated by increased annexin V-binding capacity and caspase-3 activation with flow cytometric analysis. Concentrations of 15, 20, and 40 μM curcumin significantly reduced cell proliferations. When HL-60 cells were treated with 10, 15, 20, and 40 μM concentration of curcumin, apoptotic rates were determined as 1.2, 81.1, 84.5, and 88.6%, respectively. On the incubations with the concentrations of curcumin, caspase-3 expressions (+) were found to be elevated by 8.5, 18.6, 91.2, and 92.4%, respectively. It was shown that curcumin had significant cytotoxic and apoptotic effects on HL-60 cells. It was suggested that curcumin may have a potential therapeutic role for human leukemia.
authors.affiliation: Department of Pharmacology, Anadolu University, Tepebasi, Eskisehir, Turkey. mirisd@anadolu.edu.tr
authors.forename: Miriş
authors.initials: M
authors.lastname: Dikmen
authors.forename: Zerrin
authors.initials: Z
authors.lastname: Canturk
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Ozturk
authors.forename: Yagmur
authors.initials: Y
authors.lastname: Tunali
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy
chemical_list: D053148:Caspase 3; D003474:Curcumin