title: Transcriptional regulation by HSV-1 induced HTRP via acetylation system.
abstract: The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells. HTRP was found to interact with SAP30 (mSin3A Association Protein), one of the components of co-repressor complex mSin3A, which is part of the deacetylation transfer enzyme HDAC. To reveal the biological significance of the interaction between HTRP and SAP30, real- time PCR and a dual-luciferase detecting system was used. The results indicate that HTRP could inhibit the transcription of a viral promoter, whose interaction with SAP30 synergistically affects transcriptional inhibition of the viral genes, and is related to HDAC enzyme activity. ChIP experiments demonstrate that HTRP could promote HDAC activity by increasing the deacetylation level of lysine 14 and lysine 9 in histone H3.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Yan-mei
authors.initials: YM
authors.lastname: Li
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Li
authors.forename: Long-ding
authors.initials: LD
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.forename: Rui-xiong
authors.initials: RX
authors.lastname: Na
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Wang
authors.forename: Li-chun
authors.initials: LC
authors.lastname: Wang
authors.forename: Qi-han
authors.initials: QH
authors.lastname: Li
mesh_terms: D000107:Acetylation; D002460:Cell Line; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; C113294:SAP30 protein, human; C500210:SAP30BP protein, human; D014157:Transcription Factors; D008156:Luciferases; D006655:Histone Deacetylases