title: Biomarkers of splenic function in infants with sickle cell anemia: baseline data from the BABY HUG Trial.
abstract: We evaluated spleen function in 193 children with sickle cell anemia 8 to 18 months of age by (99m)Tc sulfur-colloid liver-spleen scan and correlated results with clinical and laboratory parameters, including 2 splenic biomarkers: pitted cell counts (PIT) and quantitative Howell-Jolly bodies (HJB) enumerated by flow cytometry. Loss of splenic function began before 12 months of age in 86% of infants in association with lower total or fetal hemoglobin and higher white blood cell or reticulocyte counts, reinforcing the need for early diagnosis and diligent preventive care. PIT and HJB correlated well with each other and liver-spleen scan results. Previously described biomarker threshold values did define patients with abnormal splenic function, but our data suggest that normal spleen function is better predicted by PIT of ≤1.2% or HJB ≤55/10(6) red blood cells and absent function by PIT ≥4.5% or HJB ≥665/10(6). HJB is methodologically advantageous compared with PIT, but both are valid biomarkers of splenic function. This trial was registered at www.clinicaltrials.gov as #NCT00006400.
authors.affiliation: Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390-9063, USA. Zora.Rogers@UTSouthwestern.edu
authors.forename: Zora R
authors.initials: ZR
authors.lastname: Rogers
authors.forename: Winfred C
authors.initials: WC
authors.lastname: Wang
authors.forename: Zhaoyu
authors.initials: Z
authors.lastname: Luo
authors.forename: Rathi V
authors.initials: RV
authors.lastname: Iyer
authors.forename: Eglal
authors.initials: E
authors.lastname: Shalaby-Rana
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Dertinger
authors.forename: Barry L
authors.initials: BL
authors.lastname: Shulkin
authors.forename: John H
authors.initials: JH
authors.lastname: Miller
authors.forename: Bea
authors.initials: B
authors.lastname: Files
authors.forename: Peter A
authors.initials: PA
authors.lastname: Lane
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Thompson
authors.forename: Scott T
authors.initials: ST
authors.lastname: Miller
authors.forename: Russell E
authors.initials: RE
authors.lastname: Ware
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood / Q000473:pathology / Q000503:physiopathology*; D015415:Biomarkers / Q000378:metabolism; D004906:Erythrocyte Count; D004908:Erythrocyte Inclusions / Q000473:pathology; D005260:Female; D006801:Humans; D007223:Infant; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D013154:Spleen / Q000473:pathology / Q000503:physiopathology*
chemical_list: D015415:Biomarkers