title: SNAI1 is involved in the proliferation and migration of glioblastoma cells.
abstract: Glioblastoma is the most common type of astrocytoma in the brain. Due to its high invasiveness and chemoresistance, patients with advanced stage of glioblastoma have a poor prognosis. SNAI1, an important regulator of epithelial-mesenchymal transition, has been associated with metastasis in various carcinoma cells. However, its roles in glioblastoma cells have been poorly characterized. To examine roles of SNAI1 in glioblastoma cells, we knockdowned SNAI1 expression using siRNA. SNAI1 siRNA increased the expression level of E-cadherin and decreased that of vimentin. In the water-soluble tetrazolium salt (WST-1) assay, SNAI1 siRNA inhibited the proliferation of U87-MG and GBM05 glioblastoma cells. Moreover, in the Boyden chamber assay and Matrigel invasion assay, SNAI1 siRNA inhibited serum-induced migration and invasion of glioblastoma cells. These results suggested that SNAI1 is involved in the proliferation and migration of glioblastoma cells.
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Beomeo-Ri, Mulgeum-Eup, Yangsan, South Korea.
authors.forename: Sung-Pil
authors.initials: SP
authors.lastname: Han
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Myoung-Eun
authors.initials: ME
authors.lastname: Han
authors.forename: Hey-Eun
authors.initials: HE
authors.lastname: Sim
authors.forename: Ki-Sun
authors.initials: KS
authors.lastname: Kim
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
authors.forename: Sun-Yong
authors.initials: SY
authors.lastname: Baek
authors.forename: Bong-Seon
authors.initials: BS
authors.lastname: Kim
authors.forename: Sae-Ock
authors.initials: SO
authors.lastname: Oh
mesh_terms: D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005909:Glioblastoma / Q000473:pathology* / Q000503:physiopathology*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; D034741:RNA, Small Interfering; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D014746:Vimentin