title: Protective effect of antioxidants on blood oxidative stress caused by arginine.
abstract: In this study, we investigated in vivo and in vitro effect of arginine on parameters of oxidative stress namely thiobarbituric acid-reactive substances (TBA-RS) and total radical-trapping antioxidant parameter (TRAP) in plasma and on the antioxidant enzymes activities catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in erythrocytes of rats. Results showed that acute administration reduced TRAP and CAT activity and increased TBA-RS. Furthermore, in vitro studies did not alter oxidative parameters studied. The influence of N(ω)-nitro-L-arginine methyl ester (L-NAME) and antioxidants (α-tocopherol plus ascorbic acid) on the effects elicited by arginine was also studied. In addition, simultaneous injection of L-NAME or treatment with antioxidants prevented the alteration on TRAP, TBA-RS, and CAT activity caused by arginine. Data indicate that oxidative stress induction is probably mediated by the generation of NO and/or ONOO(-) and other free radicals, because L-NAME and these antioxidants prevented these effects caused by arginine.
authors.affiliation: Departamento de Medicina, Universidade da Região de Joinville- UNIVILLE, Campus Universitário, Bairro Bom Retiro, CEP 89201-972, Joinville, SC, Brazil.
authors.forename: Daniela Delwing
authors.initials: DD
authors.lastname: de Lima
authors.forename: Fábio
authors.initials: F
authors.lastname: Delwing
authors.forename: José Geraldo Pereira
authors.initials: JG
authors.lastname: da Cruz
authors.forename: Angela Terezinha Souza
authors.initials: AT
authors.lastname: Wyse
authors.forename: Débora
authors.initials: D
authors.lastname: Delwing-Dal Magro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001120:Arginine / Q000633:toxicity*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D001120:Arginine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D019331:NG-Nitroarginine Methyl Ester