title: The secretory response of parathyroid hormone to acute hypocalcemia in vivo is independent of parathyroid glandular sodium/potassium-ATPase activity.
abstract: The involvement of sodium/potassium-ATPase in regulating parathyroid hormone (PTH) secretion is inferred from in vitro studies. Recently, the α-klotho-dependent rapid recruitment of this ATPase to the parathyroid cell plasma membrane in response to low extracellular calcium ion was suggested to be linked to increased hormone secretion. In this study, we used an in vivo rat model to determine the importance of sodium/potassium-ATPase in PTH secretion. Glands were exposed and treated in situ with vehicle or ouabain, a specific inhibitor of sodium/potassium-ATPase. PTH secretion was significantly increased in response to ethylene glycol tetraacetic acid-induced acute hypocalcemia and to the same extent in both vehicle and ouabain groups. The glands were removed, and inhibition of the ATPase was measured by (86)rubidium uptake, which was found to be significantly decreased in ouabain-treated parathyroid glands, indicating inhibition of the ATPase. As ouabain induced systemic hyperkalemia, the effect of high potassium on hormone secretion was also examined but was found to have no effect. Thus, inhibition of the parathyroid gland sodium/potassium-ATPase activity in vivo had no effect on the secretory response to acute hypocalcemia. Hence, the suggested importance of this ATPase in the regulation of PTH secretion could not be confirmed in this in vivo model.
authors.affiliation: Nephrological Department P, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Giedre
authors.initials: G
authors.lastname: Martuseviciene
authors.forename: Jacob
authors.initials: J
authors.lastname: Hofman-Bang
authors.forename: Torben
authors.initials: T
authors.lastname: Clausen
authors.forename: Klaus
authors.initials: K
authors.lastname: Olgaard
authors.forename: Ewa
authors.initials: E
authors.lastname: Lewin
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood; D004195:Disease Models, Animal; D004533:Egtazic Acid; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006996:Hypocalcemia / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D010280:Parathyroid Glands / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000097:blood / Q000378:metabolism*; D011188:Potassium / Q000097:blood; D051381:Rats; D012414:Rubidium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D010281:Parathyroid Hormone; D012414:Rubidium Radioisotopes; D004533:Egtazic Acid; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium; D002118:Calcium