title: Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration.
abstract: Stem cell based therapy is coming of age. Besides stem cell transplantation, it has been a goal to use native autologous stem cells for tissue regeneration. However, the recruitment of native autologous stem cells at the targeting site has not been sufficient which limits the clinical application of autologous stem cells. Biomaterials have been increasingly used in tissue repair. They not only serve as scaffolds for cell proliferation, differentiation, and also provide guidance for 3-D reestablishment. In this study, we have attempted to enrich autologous stem cells at the wound site through a stem-cell-capturing collagen scaffold by conjugating with a stem cell specific antibody. Sca-1 is a common surface marker of hematopoietic, cardiac and skeletal muscle stem cells. Due to the interaction of antibody and antigen, Sca-1 positive cells could be enriched to the functional collagen scaffold both in vitro and in vivo. When the functional collagen scaffold is transplanted into C57/BL6 mouse as a patch to repair a surgical heart defect, the regeneration of cardiomyocytes has been observed. Thus, the collagen scaffolds covalently conjugated with stem cell specific antibody could be an effective approach to promote tissue regeneration.
authors.affiliation: Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Shi
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Li
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Hang
authors.initials: H
authors.lastname: Lin
authors.forename: Ling
authors.initials: L
authors.lastname: Nie
authors.forename: Dongjin
authors.initials: D
authors.lastname: Wang
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000950:Antigens, Ly / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D006801:Humans; D007202:Indicators and Reagents; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000473:pathology; D051381:Rats; D012038:Regeneration / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000906:Antibodies; D000950:Antigens, Ly; D007202:Indicators and Reagents; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D003094:Collagen