title: Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap.
abstract: In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine.
authors.affiliation: Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, Dublin, Ireland. Bazoud@tcd.ie
authors.forename: Despina
authors.initials: D
authors.lastname: Bazou
authors.forename: Roisin
authors.initials: R
authors.lastname: Kearney
authors.forename: Fiona
authors.initials: F
authors.lastname: Mansergh
authors.forename: Celine
authors.initials: C
authors.lastname: Bourdon
authors.forename: Jane
authors.initials: J
authors.lastname: Farrar
authors.forename: Michael
authors.initials: M
authors.lastname: Wride
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015398:Signal Transduction; D014465:Ultrasonics
chemical_list: D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse