title: Crystallization and preliminary X-ray studies of native and mutant intimin from enterohaemorrhagic Escherichia coli.
abstract: Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is a primarily food-borne bacterial pathogen that is capable of causing life-threatening human infections and poses a serious challenge to public health worldwide. The bacterial outer-membrane protein intimin plays a key role in the initiation process of EHEC infection. In this study, intimin from EHEC O157:H7 (Int188) and its N916Y mutant (IntN916Y) were purified and crystals of both were obtained using the hanging-drop vapour-diffusion method at 291 K. Data were collected from Int188 and IntN916Y crystals to 2.8 and 2.6 Å resolution, respectively. The crystal of Int188 belonged to the orthorhombic space group C2, with unit-cell parameters a=235.16, b=44.81, c=129.12 Å, α=γ=90, β=97.53°. The crystal of IntN916Y belonged to space group P2(1)2(1)2(1), with unit-cell parameters a=43.78, b=92.49, c=100.05 Å, α=β=γ=90°.
authors.affiliation: Department of Clinical Microbiology and Clinical Immunology, College of Medical Laboratory, Third Military Medical University, Chongqing 400038, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yi
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Xuhu
authors.initials: X
authors.lastname: Mao
authors.forename: Ming
authors.initials: M
authors.lastname: Xiao
authors.forename: Ping
authors.initials: P
authors.lastname: Luo
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Hua
authors.initials: H
authors.lastname: Jing
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Quanming
authors.initials: Q
authors.lastname: Zou
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D054324:Enterohemorrhagic Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli