title: [A preliminary study on screening for Porphyromonas gingivalis outer membrane protein antigen with two-dimensional liquid phase fractionation and matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry].
abstract: OBJECTIVE
  To screen a variety of Porphyromonas gingivalis (Pg) common outer membrane proteins with two-dimensional liquid phase fractionation (PF2D) and matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) and provide candidate target antigen for the design of vaccines with cross protection against a variety of Pg.
  
  
  METHODS
  The outer membrane proteins of Pg301, PgATCC33277 and PgW83 were extracted through ultracentrifugation, and then they were separated by ProteomeLab PF2D protein fractionation system. After separation, the outer membrane proteins were obtained through comparison, and the primary structure of the proteins was identified by MALDI-TOF/TOF-MS and database.
  
  
  RESULTS
  Ninety-nine protein samples out of 3 strains of Pg were obtained after the high performance chromato focusing (HPCF) separation process. B7 fractions of 3 strains of Pg were separated by the reversed-phase high performance liquid chromatography (RP-HPCL) separation process. After comparison of peak and retention time of chromatogram, the 8 common protein peaks of 3 strains of Pg were confirmed. The protein samples were identified by MALDI-TOF/TOF-MS, and one of them was known protein arg-gingipain A.
  
  
  CONCLUSIONS
  PF2D protein fractionation system is of good reproducibility and high resolution. A combination of PF2D and MALDI-TOF/TOF-MS can be used to identify the common outer membrane proteins of Pg.
authors.affiliation: Department of Periodontology, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Wei-hang
authors.initials: WH
authors.lastname: Si
authors.forename: Si-cen
authors.initials: SC
authors.lastname: Wang
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Guo-zhou
authors.initials: GZ
authors.lastname: Rao
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Gou
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014612:Vaccines
chemical_list: D000942:Antigens, Bacterial; D008565:Membrane Proteins; D014612:Vaccines