title: [Growth inhibition and mechanisms of human bladder cancer T24 cells by adenovirus-mediated ING4 gene in vitro].
abstract: OBJECTIVE
  To explore the inhibitory effect and anti-cancer mechanisms of adenovirus-mediated ING4 gene on the human bladder cancer T24 cells in vitro.
  
  
  METHODS
  The methods of reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of ING4 in human bladder urothelial carcinoma T24 line. The influence of Ad-ING4 transfection on cell proliferation was evaluated by MTT assay and cell apoptosis by Hochest33258 staining and flow cytometry. RT-PCR and Western blot were performed to detect the transcriptional level of such apoptosis-related genes as Bcl-2, Bax, p53, HIF-1α and caspase-3.
  
  
  RESULTS
  Human ING4 was successfully transcribed in T24 cell. Apoptosis rate of T24 cell in Ad-ING4 group was significant higher than that in control groups (17.2% ± 4.1% vs 4.7% ± 1.2% and 4.8% ± 1.2%, P < 0.05). Ad-ING4 not only up-regulated the expression of p53 and Bax(1.40 ± 0.11 vs 0.27 ± 0.04, 1.50 ± 0.12 vs 0.60 ± 0.05) and down-regulated the expression of Bcl-2 and HIF-1α (0.19 ± 0.02 vs 1.20 ± 0.08, 0.33 ± 0.03 vs 0.98 ± 0.06, all P < 0.05), but also enhanced the caspase-3 activation and eventually led to apoptosis.
  
  
  CONCLUSIONS
  Adenovirus-mediated ING4 gene exhibits anti-tumor capacity in T24 human bladder cancer cell and induces in vitro apoptosis. It may be related to the up-regulation of P53 and Bax/Bcl-2, and the down-regulation of HIF-1α. Thus the caspase-3 activation is enhanced so as to lead to apoptosis.
authors.affiliation: Department of Urology, Soochow University, Suzhou 215006, China.
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Ouyang
authors.forename: Cong-hui
authors.initials: CH
authors.lastname: Han
authors.forename: Ji-cheng
authors.initials: JC
authors.lastname: Yang
authors.forename: Hui-cui
authors.initials: HC
authors.lastname: Yang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Long
authors.forename: Xiang
authors.initials: X
authors.lastname: Ding
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; C483230:ING4 protein, human; D025521:Tumor Suppressor Proteins