title: Electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based shotgun lipidomics.
abstract: In the past decade, many new strategies for mass spectrometry (MS)-based analyses of lipids have been developed. Lipidomics is one of the most promising research fields to emerge as a result of these advances in MS. Currently, mass spectrometric analysis of lipids involves two complementary approaches: direct infusion (shotgun lipidomics) and liquid chromatography coupled to MS. In this chapter, I will demonstrate the approach of shotgun lipidomics using electrospray ionization tandem MS for the analysis of lipid molecular species directly from crude biological extracts of tissue or fluids.
authors.affiliation: Bio Separation and Mass Spectrometry, Pacific Northwest National Laboratory, Richland, WA, USA. giorgis.mezengie@pnl.gov
authors.forename: Giorgis
authors.initials: G
authors.lastname: Isaac
mesh_terms: D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D008055:Lipids / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D008099:Liver / Q000737:chemistry; D012015:Reference Standards; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D008055:Lipids; D012997:Solvents