title: Modulation of chicken macrophage effector function by T(H)1/T(H)2 cytokines.
abstract: Regulation of macrophage activity by T(H)1/2 cytokines is important to maintain the balance of immunity to provide adequate protective immunity while avoiding excessive inflammation. IFN-γ and IL-4 are the hallmark T(H)1 and T(H)2 cytokines, respectively. In avian species, information concerning regulation of macrophage activity by T(H)1/2 cytokines is limited. Here, we investigated the regulatory function of chicken T(H)1 cytokines IFN-γ, IL-18 and T(H)2 cytokines IL-4, IL-10 on the HD11 macrophage cell line. Chicken IFN-γ stimulated nitric oxide (NO) synthesis in HD11 cells and primed the cells to produce significantly greater amounts of NO when exposed to microbial agonists, lipopolysaccharide, lipoteichoic acid, peptidoglycan, CpG-ODN, and poly I:C. In contrast, chicken IL-4 exhibited bi-directional immune regulatory activity: it activated macrophage NO synthesis in the absence of inflammatory agonists, but inhibited NO production by macrophages in response to microbial agonists. Both IFN-γ and IL-4, however, enhanced oxidative burst activity of the HD11 cells when exposed to Salmonella enteritidis. IL-18 and IL-10 did not affect NO production nor oxidative burst in HD11 cells. Phagocytosis and bacterial killing by the HD11 cells were not affected by the treatments of these cytokines. Infection of HD11 cells with S.enteritidis was shown to completely abolish NO production regardless of IFN-γ treatment. This study has demonstrated that IFN-γ and IL-4 are important T(H)1 and T(H)2 cytokines that regulate macrophage function in chickens.
authors.affiliation: Southern Plain Agricultural Research Center, USDA-ARS, 2881 F&B Road, College Station, TX 77845, United States. haiqi.he@ars.usda.gov
authors.forename: Haiqi
authors.initials: H
authors.lastname: He
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Genovese
authors.forename: Michael H
authors.initials: MH
authors.lastname: Kogut
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D054884:Host-Pathogen Interactions; D007371:Interferon-gamma / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology; D020382:Interleukin-18 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D009569:Nitric Oxide / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010457:Peptidoglycan / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D011070:Poly I-C / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects; D012477:Salmonella enteritidis / Q000502:physiology; D013682:Teichoic Acids / Q000494:pharmacology; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: C408982:CPG-oligonucleotide; D020382:Interleukin-18; D008070:Lipopolysaccharides; D009838:Oligodeoxyribonucleotides; D010457:Peptidoglycan; D013682:Teichoic Acids; D016753:Interleukin-10; D015847:Interleukin-4; D009569:Nitric Oxide; C009900:lipoteichoic acid; D007371:Interferon-gamma; D011070:Poly I-C