title: The association of plasma prorenin level with an oxidative stress marker, 8-OHdG, in nondiabetic hemodialysis patients.
abstract: BACKGROUND
  Circulating prorenin contributes to the pathogenesis of tissue damage leading to cardiovascular disease (CVD) in hypertension and diabetic mellitus (DM) by activating the tissue renin-angiotensin-aldosterone (RAS) system; however, little is known about its roles in hemodialysis (HD) patients.
  
  
  METHODS
  We evaluated plasma prorenin level and prorenin receptor [(P)RR] expression in peripheral blood mononuclear cells (PBMCs) in 49 nondiabetic HD (non-DM-HD) patients. Then we investigated the association between plasma prorenin level or (P)RR expression level in PBMCs and CVD-predictive biomarkers.
  
  
  RESULTS
  The plasma prorenin level increased in non-DM-HD patients [147.1 ± 118.9 pg/ml (standard value <100 pg/ml)]. The (P)RR mRNA expression level in PBMCs also increased 1.41 ± 0.39-fold in non-DM-HD patients compared with that in healthy control subjects (p < 0.001). Although plasma prorenin level did not correlate with plasma BNP level and plasma high-sensitivity C-reactive protein level, it significantly correlated with plasma 8-hydroxydeoxyguanosine (8-OHdG) level (r = 0.535, p < 0.001). The plasma prorenin level did not correlate with plasma renin activity (PRA), plasma angiotensin I (AT I) level, plasma angiotensin II (AT II) level and plasma aldosterone (Ald) level. PRA, plasma AT I level, plasma AT II level and plasma Ald level did not correlate with the level of any CVD predictive biomarker. (P)RR expression level in PBMCs did not correlate with the level of any CVD predictive biomarker.
  
  
  CONCLUSIONS
  The plasma prorenin level and (P)RR expression level in PBMCs increased, and the plasma prorenin level was associated with plasma 8-OHdG level independent of circulating RAS in non-DM-HD patients.
authors.affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. ymori@jichi.ac.jp.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Morishita
authors.affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Hanawa
authors.affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Miki
authors.affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Sugase
authors.affiliation: Ninomiya Central Clinic, Tochigi, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sugaya
authors.affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Chinda
authors.affiliation: Kumakura Clinic, Tochigi, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Iimura
authors.affiliation: Yuki Clinic, Ibaraki, Japan.
authors.forename: Sadao
authors.initials: S
authors.lastname: Tsunematsu
authors.affiliation: Department of Medical Physiology, Meiji Pharmaceutical University, Tokyo, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishibashi
authors.affiliation: Division of Nephrology, Department of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kusano
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000097:blood; D018384:Oxidative Stress; D011956:Receptors, Cell Surface / Q000097:blood; D006435:Renal Dialysis; D012083:Renin / Q000097:blood*; D000093342:Prorenin Receptor
chemical_list: D015415:Biomarkers; D011956:Receptors, Cell Surface; D020097:Natriuretic Peptide, Brain; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002097:C-Reactive Protein; D012083:Renin; D003849:Deoxyguanosine; D000093342:Prorenin Receptor