title: Investigation of nutrient feeding strategies in a countercurrent mixed-acid multi-staged fermentation: experimental data.
abstract: Nutrients are essential for microbial growth and metabolism in mixed-culture acid fermentations. Understanding the influence of nutrient feeding strategies on fermentation performance is necessary for optimization. For a four-bottle fermentation train, five nutrient contacting patterns (single-point nutrient addition to fermentors F1, F2, F3, and F4 and multi-point parallel addition) were investigated. Compared to the traditional nutrient contacting method (all nutrients fed to F1), the near-optimal feeding strategies improved exit yield, culture yield, process yield, exit acetate-equivalent yield, conversion, and total acid productivity by approximately 31%, 39%, 46%, 31%, 100%, and 19%, respectively. There was no statistical improvement in total acid concentration. The traditional nutrient feeding strategy had the highest selectivity and acetate-equivalent selectivity. Total acid productivity depends on carbon-nitrogen ratio.
authors.affiliation: Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA. AaronSmith328@gmail.com
authors.forename: Aaron Douglas
authors.initials: AD
authors.lastname: Smith
authors.forename: Nur Ain
authors.initials: NA
authors.lastname: Lockman
authors.forename: Mark T
authors.initials: MT
authors.lastname: Holtzapple
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D005285:Fermentation; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013696:Temperature
chemical_list: D002264:Carboxylic Acids; D002244:Carbon; D009584:Nitrogen