title: Glycosylation pattern in the appendix testis in children with cryptorchidism.
abstract: In humans, at about week 6, sex cords develop within the forming testes. Testes normally descend to the scrotum; cryptorchidism occurs when one or two testes do not descend to scrotum and in some case are accompanied by the appendix testis. The appendix testis is a small sessile or polypoid structure located at the antero superior pole of the testis, adjacent to the head of the epididymis. Glycans can be involved in development of the appendix testis and cryptorchidism. In this work, lectin histochemistry was used to evaluate glycans expression in appendix testis in children with cryptorchidism. Our results showed that lectin from Lens culinaris, Ulex europaeus I., Canavalia ensiformis, Artocarpus integrifolia, Glycine max, and Griffonia simplicifolia recognizes epithelial and estromal cells. Not interaction was observed with lectin from Amaranthus leucocarpus, while lectin from Dolichus biflorus lectin only recognizes epithelial cells. Our results suggest that O-glycans linked in some glycoproteins represent important elements in appendix testis development.
authors.affiliation: Centro de Investigaciones en Ciencias Medicas y Biológicas Facultad de Medicina, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Lopez
authors.forename: Salvador
authors.initials: S
authors.lastname: Jmenez
authors.forename: Ruth
authors.initials: R
authors.lastname: Martinez
authors.forename: Maria del Socorro
authors.initials: Mdel S
authors.lastname: Pina
authors.forename: Belem
authors.initials: B
authors.lastname: Gallegos
authors.forename: Eduardo
authors.initials: E
authors.lastname: Pérez-Campos
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Pedro
authors.initials: P
authors.lastname: Hernández
mesh_terms: D002648:Child; D003456:Cryptorchidism / Q000196:embryology / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D037121:Plant Lectins; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D017154:Stromal Cells / Q000737:chemistry / Q000378:metabolism*; D013737:Testis / Q000196:embryology / Q000378:metabolism* / Q000473:pathology
chemical_list: D006023:Glycoproteins; D037121:Plant Lectins; D011134:Polysaccharides