title: Effect of water-soluble P-chitosan and S-chitosan on human primary osteoblasts and giant cell tumor of bone stromal cells.
abstract: Water-soluble phosphorylated chitosan (P-chitosan) and disodium (1 → 4)-2-deoxy-2-sulfoamino-β-D-glucopyranuronan (S-chitosan) are two chemically modified chitosans. In this study, we found that P-chitosan significantly promotes cell proliferation of both human primary osteoblasts (OBs) and the OB like stromal cell component of the giant cell tumor of bone (GCTB) cells at the concentration from 125 to 1000 µg ml⁻¹ at all time points of 1, 3, 5 and 7 days after treatment. Further investigation of the osteogenic effect of the P-chitosan suggested that it regulates the levels of osteoclastogenic factors, receptor activator of nuclear factor kappa B ligand and osteoprotegerin expression. An interesting finding is that S-chitosan at lower concentration (100 µg ml⁻¹) stimulates cell proliferation while a higher dose (1000 µg ml⁻¹) of S-chitosan inhibits it. The inhibitory effect of S-chitosan on human primary GCT stromal cells was greater than that of OBs (p < 0.05). Taken together, our findings elucidated the osteogenic effect of P-chitosan and the varying effects of S-chitosan on the proliferation of human primary OBs and GCT stromal cells and provided us the rationale for the construction of novel bone repair biomaterials with the dual properties of bone induction and bone tumor inhibition.
authors.affiliation: Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Tang
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: Carol P Y
authors.initials: CP
authors.lastname: Lau
authors.forename: L Z
authors.initials: LZ
authors.lastname: Zheng
authors.forename: X H
authors.initials: XH
authors.lastname: Xie
authors.forename: X L
authors.initials: XL
authors.lastname: Wang
authors.forename: X H
authors.initials: XH
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Patrick
authors.forename: L
authors.initials: L
authors.lastname: Qin
authors.forename: Shekhar M
authors.initials: SM
authors.lastname: Kumta
mesh_terms: D001483:Base Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018212:Giant Cell Tumor of Bone / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D014867:Water
chemical_list: D001672:Biocompatible Materials; D017931:DNA Primers; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D014867:Water; D048271:Chitosan