title: Preparation of 3D fibroin/chitosan blend porous scaffold for tissue engineering via a simplified method.
abstract: In this work, we developed a simple and flexible method to manufacture a 3D porous scaffold based on the blend of regenerated silk fibroin (RSF) and chitosan (CS). No crosslinker or other toxic reagents were used in this method. The pores of resulted 3D scaffolds were connected with each other, and their sizes could be easily controlled by the concentration of the mixed solution. Compared with pure RSF scaffolds, the water absorptivities of these RSF/CS blend scaffolds with significantly enhanced mechanical properties were greatly increased. The results of MTT and RT-PCR tests indicated that the chondrocytes grew very well in these blend RSF/CS porous scaffolds. This suggested that the RSF/CS blend scaffold prepared by this new method could be a promising candidate for applications in tissue engineering.
authors.affiliation: The Key Laboratory of Molecular Engineering of Polymers of MOE, Department of Macromolecular Science, The Laboratory of Advanced Materials, Fudan University, Shanghai 200433, PR China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Ruan
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Jinrong
authors.initials: J
authors.lastname: Yao
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000138:chemical synthesis* / Q000494:pharmacology; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005349:Fibroins / Q000138:chemical synthesis* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016062:Porosity / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water
chemical_list: D001672:Biocompatible Materials; D012333:RNA, Messenger; D012996:Solutions; D014867:Water; D005349:Fibroins; D048271:Chitosan