title: Different patterns of evolution in the centromeric and telomeric regions of group A and B haplotypes of the human killer cell Ig-like receptor locus.
abstract: The fast evolving human KIR gene family encodes variable lymphocyte receptors specific for polymorphic HLA class I determinants. Nucleotide sequences for 24 representative human KIR haplotypes were determined. With three previously defined haplotypes, this gave a set of 12 group A and 15 group B haplotypes for assessment of KIR variation. The seven gene-content haplotypes are all combinations of four centromeric and two telomeric motifs. 2DL5, 2DS5 and 2DS3 can be present in centromeric and telomeric locations. With one exception, haplotypes having identical gene content differed in their combinations of KIR alleles. Sequence diversity varied between haplotype groups and between centromeric and telomeric halves of the KIR locus. The most variable A haplotype genes are in the telomeric half, whereas the most variable genes characterizing B haplotypes are in the centromeric half. Of the highly polymorphic genes, only the 3DL3 framework gene exhibits a similar diversity when carried by A and B haplotypes. Phylogenetic analysis and divergence time estimates, point to the centromeric gene-content motifs that distinguish A and B haplotypes having emerged ~6 million years ago, contemporaneously with the separation of human and chimpanzee ancestors. In contrast, the telomeric motifs that distinguish A and B haplotypes emerged more recently, ~1.7 million years ago, before the emergence of Homo sapiens. Thus the centromeric and telomeric motifs that typify A and B haplotypes have likely been present throughout human evolution. The results suggest the common ancestor of A and B haplotypes combined a B-like centromeric region with an A-like telomeric region.
authors.affiliation: Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
authors.forename: Chul-Woo
authors.initials: CW
authors.lastname: Pyo
authors.forename: Lisbeth A
authors.initials: LA
authors.lastname: Guethlein
authors.forename: Quyen
authors.initials: Q
authors.lastname: Vu
authors.forename: Ruihan
authors.initials: R
authors.lastname: Wang
authors.forename: Laurent
authors.initials: L
authors.lastname: Abi-Rached
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Norman
authors.forename: Steven G E
authors.initials: SG
authors.lastname: Marsh
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Miller
authors.forename: Peter
authors.initials: P
authors.lastname: Parham
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Geraghty
mesh_terms: D000483:Alleles; D020816:Amino Acid Motifs; D002503:Centromere / Q000648:ultrastructure*; D015723:Gene Library; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D010802:Phylogeny; D054340:Receptors, KIR / Q000235:genetics*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000648:ultrastructure*
chemical_list: D007136:Immunoglobulins; D054340:Receptors, KIR