title: [Effects of rat serum containing Chinese herbal medicine Sangen Decoction on osteoclastogenesis and bone resorption of osteoclasts induced by polymethylmethacrylate particles].
abstract: OBJECTIVE
  To investigate the effects of Sangen Decoction, a compound Chinese herbal medicine, on osteoclastogenesis and bone resorption function of osteoclasts induced by polymethylmethacrylate particles in vitro.
  
  
  METHODS
  Macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) were used to induce differentiation of bone marrow-derived macrophages (BMMs) towards osteoclasts. BMMs and polymethylmethacrylate particles with ratio of 1:3 were added to the 24-well plate and 96-well plate with bone slices respectively. A total of 50 male SD rats were divided into 5 groups randomly with each group containing 10 rats. After being treated with different drugs, serum samples of rats in each group were extracted, i.e., the blank serum, Western medicine (ibandronate) serum and high-, medium-, and low-dose Sangen Decoction serum and were added to the medium respectively. The tartrate-resistant acid phosphatase (TRAP) staining was used to identify the differentiation of BMMs and for counting of osteoclasts. Area of lacuna induced by osteoclast bone resorption on the bone slices was measured by computer image processing.
  
  
  RESULTS
  Numbers of osteoclasts of treatment groups were less than that of blank group by TRAP staining (P<0.05); numbers of osteoclasts of positive control group and high-dose Sangen Decoction group were much lower than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05). In bone resorption assay, area of lacuna of blank group was larger than those of treatment groups (P<0.05); areas of lacuna of Western medicine group and high-dose Sangen Decoction group were much smaller than those of medium- and low-dose Sangen Decoction groups (P<0.05), and no difference was found between Western medicine group and high-dose Sangen Decoction group (P>0.05).
  
  
  CONCLUSIONS
  Sangen Decoction can inhibit osteoclastogenesis induced by polymethylmethacrylate particles as well as bone resorption function of osteoclasts.
authors.affiliation: Department of Orthopaedics and Traumatology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China.
authors.forename: Shu-qiang
authors.initials: SQ
authors.lastname: Wang
authors.forename: Wu
authors.initials: W
authors.lastname: Rao
authors.forename: Lian-fu
authors.initials: LF
authors.lastname: Deng
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Zhu
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D010010:Osteoclasts / Q000187:drug effects* / Q000502:physiology; D019904:Polymethyl Methacrylate / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum
chemical_list: D004365:Drugs, Chinese Herbal; D019904:Polymethyl Methacrylate