title: Angiotensinogen metabolism in rat aorta: robust formation of proangiotensin-12.
abstract: Renin-angiotensin system (RAS) plays important role in the regulation of vessel wall homeostasis. Proangiotensin-12 (proAng-12, Ang-(1-12)) is a newly characterized metabolite of angiotensinogen, formed in array of organs of rats, which may serve as an alternate substrate for local angiotensin production, by-passing the traditional renin-dependent conversion of angiotensinogen to angiotensin I. In this work using LC/MS method we identified proAng-12 as a main product of angiotensinogen metabolism ex vivo, in organ-bath of rat aortic tissue. In this setting, proAng-12 appeared to be not only prevalent metabolite of angiotensinogen, but also served as a substrate for generation of Ang I and subsequently, Ang II. The functional significance of this surprising finding requires further investigation.
authors.affiliation: Jagiellonian University Medical College, Cracow, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Bujak-Gizycka
authors.forename: R
authors.initials: R
authors.lastname: Olszanecki
authors.forename: M
authors.initials: M
authors.lastname: Suski
authors.forename: J
authors.initials: J
authors.lastname: Madek
authors.forename: A
authors.initials: A
authors.lastname: Stachowicz
authors.forename: R
authors.initials: R
authors.lastname: Korbut
mesh_terms: D000803:Angiotensin I / Q000096:biosynthesis / Q000378:metabolism; D000804:Angiotensin II / Q000096:biosynthesis / Q000378:metabolism; D000808:Angiotensinogen / Q000096:biosynthesis / Q000378:metabolism*; D000809:Angiotensins / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D008297:Male; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000378:metabolism; D012084:Renin-Angiotensin System / Q000502:physiology
chemical_list: D000809:Angiotensins; D010446:Peptide Fragments; C515557:proangiotensin-12, rat; D000808:Angiotensinogen; D000804:Angiotensin II; D000803:Angiotensin I; D012083:Renin