title: [Effect of hepatitis B virus X gene on the expression of spastic paraplegia 21].
abstract: OBJECTIVE
  To investigate the effect of hepatitis B virus(HBV) X gene on the expression of SPG21.
  
  
  METHODS
  The expressions of SPG21 mRNA and protein in HepG2 and HepG2.2.15 cells were tested by RT-PCR and western blot. HepG2 cells were co-transfected with reporter plasmid pGL3-SPG21 and plasmids carrying individual genes of HBV, the luciferase activity was measured and the expressions of SPG21 were detected by RT-PCR and western blot.
  
  
  RESULTS
  The expressions of SPG21 mRNA and protein were higher in HepG2.2.15 cells than in HepG2 cells (0.36+/-0.06 vs 0.21+/-0.05, P value is less than 0.05). The activity of SPG21 in HepG2 cells transfected with pCMV-X was higher (875+/-27 vs 67+/-12, P value is less than 0.01) as compared to blank control group (transfected with pCMV-tag2B). HBV X gene enhanced SPG21 gene promoter activity, SPG21 mRNA expression and SPG21 protein production in HepG2 cells in a dose-dependent manner.
  
  
  CONCLUSIONS
  HBV X gene can specially activate SPG21 expression.
authors.affiliation: The Infectious Disease Hospital of Ningbo, Ningbo 315010, China.
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Gao
authors.forename: Peng-jian
authors.initials: PJ
authors.lastname: Weng
authors.forename: Reng-bin
authors.initials: RB
authors.lastname: Ji
authors.forename: De-zhou
authors.initials: DZ
authors.lastname: Li
authors.forename: Yong-yan
authors.initials: YY
authors.lastname: Li
authors.forename: Hong-shan
authors.initials: HS
authors.lastname: Li
authors.forename: Shi-xiong
authors.initials: SX
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004279:DNA, Viral; D012333:RNA, Messenger; C424703:SPG21 protein, human; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein