title: Detection and molecular characterization of human group C rotavirus in Brazil.
abstract: OBJECTIVE
  The aim of the present study was to characterize 24 isolates of group C rotavirus (RV-C) by analysis of DNA sequences of the VP4, VP6, VP7 and NSP4 genes of several Brazilian states.
  
  
  METHODS
  All RV-C strains were confirmed by polyacrylamide gel electrophoresis and were characterized by sequence and phylogenetic analysis of the genes encoding NSP4, VP6, VP4, and VP7 proteins.
  
  
  RESULTS
  Analysis of the NSP4 gene from Brazilian strains revealed that the isolates are more closely related to each other than to those of other strains previously published. The VP6 gene showed high homology to Indian strains. The sequences of VP4 and VP7 genes showed two lineages circulating in Brazil in the same region and at the same time.
  
  
  CONCLUSIONS
  These results confirm the transmission of RV-C in Brazil. RV-C infection appears to occur occasionally despite the low detection rate of the virus.
authors.affiliation: Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fiocruz, Manguinhos, Brazil.
authors.forename: Irene Trigueiros
authors.initials: IT
authors.lastname: Araújo
authors.forename: Marcos Bryan
authors.initials: MB
authors.lastname: Heinemann
authors.forename: Alexandre Madi
authors.initials: AM
authors.lastname: Fialho
authors.forename: José Paulo Gagliardi
authors.initials: JP
authors.lastname: Leite
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins