title: Nano-CuO inhibited voltage-gated sodium current of hippocampal CA1 neurons via reactive oxygen species but independent from G-proteins pathway.
abstract: The aim of this study was to investigate the effects of nano-CuO on voltage-gated sodium current (I(Na) ) in hippocampal CA1 neurons. The results suggested that nano-CuO inhibited amplitudes of I(Na) currents and prolonged peak rise time of action potential (AP). The responses to nano-CuO were inhibited by reactive oxygen species (ROS) scavenger. However, the G-proteins inhibitor did not block the effects of nano-CuO. The effects of nano-CuO were mediated through activation of ROS-I(Na) -AP signaling cascades and independent from G-proteins pathway.
authors.affiliation: College of Medicine, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Liu
authors.forename: Shichang
authors.initials: S
authors.lastname: Liu
authors.forename: Guogang
authors.initials: G
authors.lastname: Ren
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D004336:Drug Antagonism; D016166:Free Radical Scavengers / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D026941:Sodium Channel Blockers / Q000633:toxicity*; D015222:Sodium Channels / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D003300:Copper; D019204:GTP-Binding Proteins; C000520:cuprous oxide