title: [Dynamic alterations of VEGF and intervention of its expression on effect of hepatocyte malignant transformation].
abstract: OBJECTIVE
  To investigate the influences of VEGF expression through the intervention of thalidomide in malignant transformation of hepatocytes.
  
  
  METHODS
  Hepatoma model was induced with 2-fluorenyl-acetamide (2-FAA, 0.05%) in male SD rats. And thalidomide was administered intragastrically to block the progress of hepatoma. Some rats were sacrificed at a fortnightly interval. Morphological changes were observed by pathological examinations (HE staining). The VEGF expressions in rat liver tissues were detected by ELISA and immunohistochemistry respectively.
  
  
  RESULTS
  Hepatocytes in rats fed with 2-FAA showed vacuole-like denaturations at an early stage, dysplastic nodules appeared at a middle stage and finally progressed to tubercles of cancerous nest. All were the manifestations of highly differentiated hepatocellular carcinoma (HCC). An increasing tendency of hepatic VEGF protein was found for normal liver to precancerous to cancerous tissues during the development of hepatoma. The VEGF levels in hepatoma were significantly higher than those in normal ones. Thalidomide repressed the morphologic change of hepatic cells. And the VEGF level of thalidomide group was lower than those in 2-FAA group.
  
  
  CONCLUSIONS
  Thalidomide can inhibit the hepatic VEGF expression and arrest the development of rat hepatoma.
authors.affiliation: Research Center of Clinical Medicine, Nantong University, Nantong 226001, China. yaodf@ahnmc.com
authors.forename: Deng-fu
authors.initials: DF
authors.lastname: Yao
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Qiu
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Ning-hua
authors.initials: NH
authors.lastname: Yao
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Li
authors.forename: Yin-zhu
authors.initials: YZ
authors.lastname: Bian
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat