title: [Anti-tumor activities of lung cancer dendritic cell vaccine modified by mutant Ki-ras gene in vitro].
abstract: BACKGROUND
  To construct a DC-Ad-Ki-ras(V12) vaccine and investigate the anti-tumor activities of lung cancer dendritic cell vaccine modified by mutant Ki-ras gene in vitro.
  
  
  METHODS
  Ki-ras(V12) cDNA was transfected into cultured bone marrow-derived DC with the recombinant adenovirus [(Ad-Ki-ras(V12)] containing human mutant Ki-ras gene. Anti-tumor activity of the vaccine was studied in vitro by flow cytometry, PCR, MLR and cytotoxicity assay.
  
  
  RESULTS
  (1) The DC vaccine was confirmed not only to express Ki-ras(V12) gene, but also to remarkably stimulate lymphocyte proliferation and improve CTL activity. (2) The DC vaccine modified by mutant Ki-ras gene could induce specifical CTL activity of immunized mice against Lewis lung carcinoma that could express Ki-ras(V12) gene, but not to B16.
  
  
  CONCLUSIONS
  The DC vaccine modified by mutant Ki-ras gene can induce obvious anti-tumor activities against Lewis lung carcinoma that can express Ki-ras(V12) gene.
authors.affiliation: Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
authors.forename: Jingrui
authors.initials: J
authors.lastname: Yu
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Lu
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Chen
authors.forename: Cheng
authors.initials: C
authors.lastname: Yi
authors.forename: Deyun
authors.initials: D
authors.lastname: Luo
authors.forename: Michael T
authors.initials: MT
authors.lastname: Lotze
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou