title: Identification of differential expressed transcripts in cervical cancer of Mexican patients.
abstract: The aim of this study was to identify the gene expression profile in biopsies of patients with cervical intraepithelial neoplasia (CIN) 1, CIN 2, CIN 3, and microinvasive cancer by suppression subtractive hybridization and Southern blotting. After analyzing 1,800 cDNA clones, we found 198 upregulated genes, 166 downregulated, and no significant change of gene expression in 86 clones (p = 0.005). These results were validated by Northern blot analysis (p = 0.0001) in the identification of 28 overexpressed and 7 downregulated transcripts. We observed a set of genes related to the Notch signaling pathway that may be involved in the transformation of cervical cells and in the development to malignancy. The differentially expressed genes may provide useful information about the molecular mechanisms involved in human cervical carcinoma and as diagnostic markers.
authors.affiliation: División de Biologia Molecular, Instituto Potosino de Investigación Cientifica y Tecnologica AC, San Luis Potosi, Mexico. lsantos@ipicyt.edu.mx
authors.forename: Leticia
authors.initials: L
authors.lastname: Santos
authors.forename: Ma Fabiola
authors.initials: MF
authors.lastname: León-Galván
authors.forename: Erika Nahomy
authors.initials: EN
authors.lastname: Marino-Marmolejo
authors.forename: Ana Paulina
authors.initials: AP
authors.lastname: Barba de la Rosa
authors.forename: Antonio
authors.initials: A
authors.lastname: De León Rodríguez
authors.forename: Roberto
authors.initials: R
authors.lastname: González-Amaro
authors.forename: Ramón Gerardo
authors.initials: RG
authors.lastname: Guevara-González
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D008800:Mexico; D000071447:N-Myc Proto-Oncogene Protein; D009687:Nuclear Proteins / Q000235:genetics; D015513:Oncogene Proteins / Q000235:genetics; D000071656:Receptor, Notch3; D051880:Receptors, Notch / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D051017:Apoptosis Regulatory Proteins; C477529:CCAR1 protein, human; D018797:Cell Cycle Proteins; C459801:MYCN protein, human; D000071447:N-Myc Proto-Oncogene Protein; C499374:NOTCH3 protein, human; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D000071656:Receptor, Notch3; D051880:Receptors, Notch