title: Chemotherapy with hybrid liposomes for acute lymphatic leukemia leading to apoptosis in vivo.
abstract: Hybrid liposomes (HL) composed of 95 mol% l-α-dimyristoylphosphatidylcholine (DMPC) and 5 mol% polyoxyethylene (25) dodecyl ether (C(12)(EO)(25)) were prepared by sonication. A clear solutions of HL-25 having hydrodynamic diameter of about 50 nm could be maintained over 3 weeks. Remarkable reduction of tumor volume in model mice of acute lymphatic leukemia (ALL) intravenously treated with HL-25 without drugs after the subcutaneously inoculation of human ALL (MOLT-4) cells was verified in vivo. Induction of apoptosis in tumor of model mice of ALL treated with HL-25 was observed in micrographs on the basis of TUNEL method. Remarkable decrease of the ascites in ALL model mice treated with HL-25 was observed. It is noteworthy that prolonged survival (>400%) was obtained in model mice of ALL after the treatment with HL-25 without drugs.
authors.affiliation: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ichihara
authors.forename: Junichi
authors.initials: J
authors.lastname: Ueno
authors.forename: Masayo
authors.initials: M
authors.lastname: Umebayashi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Ueoka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D004134:Dimyristoylphosphatidylcholine / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D008081:Liposomes; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D016513:Mice, SCID; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000473:pathology; D016019:Survival Analysis; D023041:Xenograft Model Antitumor Assays
chemical_list: D008081:Liposomes; C558492:polyoxyethylene(25)dodecyl ether; D011092:Polyethylene Glycols; D004134:Dimyristoylphosphatidylcholine