title: Pep-1 Peptide-modified liposomal carriers for intracellular delivery of gold nanoparticles.
abstract: A Pep-1 peptide-modified liposomal (Pep1-Lipo) carrier system was investigated to increase the intracellular delivery of gold nanoparticles (Au NPs). Au NPs with a mean diameter of 13 nm were successfully encapsulated into the inner aqueous compartment of the novel carrier using an ethanol injection technique, reserving the distinctive optical characteristics of the surface plasmon resonance peak around 530 nm. The Au NP-loaded liposomal carrier was physically characterized as 150-170 nm in size and 45 mV in zeta potential. Dark field microscopic observation demonstrated that in vitro cellular association and/or translocation of the nanoprobes into the cells was increased by Pep1-Lipo carriers compared to bare Au NPs. In conclusion, this novel liposomal formulation is a promising platform for the intracellular delivery of metallic nanoprobes including Au NPs.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Kang
authors.forename: Sangyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Bo Kyun
authors.initials: BK
authors.lastname: Kim
authors.forename: Jae Yoon
authors.initials: JY
authors.lastname: Eum
authors.forename: Sang Han
authors.initials: SH
authors.lastname: Park
authors.forename: Min Hyung
authors.initials: MH
authors.lastname: Kang
authors.forename: Chil Hwan
authors.initials: CH
authors.lastname: Oh
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Choi
mesh_terms: D045744:Cell Line, Tumor; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D008081:Liposomes; C503924:Pep-1 peptide; D010455:Peptides; D000431:Ethanol; D003543:Cysteamine; D006046:Gold