title: Monoclonal antibody to CD31 (PECAM-1) inhibits tubular regeneration after ischemia reperfusion injury in the rat.
abstract: OBJECTIVE
  We used a rat model of renal ischemia (35 min) to test the potential involvement of platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31) in the process of S3 tubule regeneration.
  
  
  METHODS
  A monoclonal antibody specific for murine PECAM-1 was injected i.p. immediately after kidney reperfusion or 48 h post-ischemia. One day before ischemia, each animal received an i.p. injection of 80 mg/kg 5-bromo-2'-deoxyuridine (BrdU). Experimental animals were sacrificed 1, 2, 3, 7 and 14 days post-ischemia. Renal sections were processed to characterize the histopathological alterations and the distribution of BrdU-immunopositive cells.
  
  
  RESULTS
  Our observations showed that anti-PECAM-1 administration was associated with an inhibition of S3 tubule regeneration along with a progressive cystic dilatation of renal tubules that was particularly prominent 2 weeks post-ischemia. Interestingly, injection of anti-PECAM-1 48 h post-ischemia failed to block renal regeneration and was followed by a normal re-epithelialization of S3 tubules.
  
  
  CONCLUSIONS
  Our data showed that the blockade of PECAM-1 immediately after kidney reperfusion inhibits tubular regeneration. These observations suggest that transendothelial migration of extrarenal cells could be a precocious and pivotal step in kidney reparation, but also suggest that these extrarenal cells could be essential to the process of tubular regeneration.
authors.affiliation: Laboratory of Histology, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium.
authors.forename: Xavier
authors.initials: X
authors.lastname: Valentini
authors.forename: Annabel
authors.initials: A
authors.lastname: Gossiaux
authors.forename: Nathalie
authors.initials: N
authors.lastname: Caron
authors.forename: Denis
authors.initials: D
authors.lastname: Nonclercq
authors.forename: Alexandre
authors.initials: A
authors.lastname: Legrand
authors.forename: Gérard
authors.initials: G
authors.lastname: Toubeau
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000494:pharmacology*; D007683:Kidney Tubular Necrosis, Acute / Q000276:immunology / Q000473:pathology; D007684:Kidney Tubules / Q000502:physiology*; D008297:Male; D051379:Mice; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects / Q000276:immunology; D015427:Reperfusion Injury / Q000188:drug therapy / Q000276:immunology*; D057705:Transendothelial and Transepithelial Migration
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D019408:Platelet Endothelial Cell Adhesion Molecule-1