title: Protection of cellular DNA and membrane from γ-radiation-induced damages and enhancement in DNA repair by sesamol.
abstract: Sesamol (SM), a nutritional phenolic antioxidant compound present in sesame seeds, protected pBR 322 DNA from gamma radiation-induced damages. SM prevented gamma radiation-induced degradation of covalently closed circular form of plasmid DNA in a concentration-dependent manner. Also SM protected cellular DNA of mouse blood leukocytes exposed to 4 Gy gamma radiation, ex vivo, as revealed by the data from alkaline comet assay studies. SM (5 mM) showed a faster time-dependant decrease of the radiation-induced DNA damage in mouse blood leukocytes following postirradiation incubation ex vivo, which could be attributed to enhanced DNA repair. SM protected the biomembranes from radiation-induced lipid peroxidation. Thus, SM could act as a radioprotector for the biomembranes and cellular DNA against the deleterious effects of ionizing radiation.
authors.affiliation: Department of Radiation Biology, Amala Cancer Research Centre, Thrissur, Kerala, India.
authors.forename: Gopakumar Gopinathan
authors.initials: GG
authors.lastname: Nair
authors.forename: Cherupally Krishnan Krishnan
authors.initials: CK
authors.lastname: Nair
mesh_terms: D000818:Animals; D052117:Benzodioxoles / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000528:radiation effects*; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism; D008563:Membrane Lipids / Q000528:radiation effects; D051379:Mice; D008815:Mice, Inbred Strains; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D010636:Phenols / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*
chemical_list: D052117:Benzodioxoles; D008563:Membrane Lipids; D010636:Phenols; D011837:Radiation-Protective Agents; D008315:Malondialdehyde; D004247:DNA; C025583:sesamol