title: Characterization of histone H3K27 modifications in the β-globin locus.
abstract: Histone H3K27 is acetylated or methylated in the environment of nuclear chromatin. Here, to characterize the modification pattern of H3K27 in locus control region (LCR) and to understand the correlation of various H3K27 modifications with transcriptional activity of genes, we analyzed the human β-globin locus using the ChIP assay. The LCR of the human β-globin locus was enriched by H3K27ac and H3K27me1 in erythroid K562 cells. The highly transcribed globin genes were hyperacetylated at H3K27, but the repressed globin genes were highly dimethylated at this lysine in these cells. However, in non-erythroid 293FT cells, the β-globin locus was marked by a high level of H3K27me3. EZH2 and SUZ12, subunits of polycomb repressive complex 2, were comparably detected with the H3K27me3 pattern in K562 and 293FT cells. In addition, H3K27ac, H3K27me1 and H3K27me3 were established in an enhancer-dependent manner in a model minichromosomal locus containing an enhancer and its target gene. Taken together, these results show that H3K27 modifications have distinctive correlations with the chromatin state or transcription level of genes and are influenced by an enhancer.
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea.
authors.forename: Yea Woon
authors.initials: YW
authors.lastname: Kim
authors.forename: AeRi
authors.initials: A
authors.lastname: Kim
mesh_terms: D000107:Acetylation; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004742:Enhancer Elements, Genetic; D020868:Gene Silencing; D006657:Histones / Q000378:metabolism*; D006801:Humans; D019901:Locus Control Region; D008239:Lysine / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D014158:Transcription, Genetic; D055544:beta-Globins / Q000235:genetics*
chemical_list: D002843:Chromatin; D006657:Histones; D055544:beta-Globins; D008239:Lysine