title: ApoA-II directs morphogenetic movements of zebrafish embryo by preventing chromosome fusion during nuclear division in yolk syncytial layer.
abstract: The high density lipoprotein (HDL) represents a class of lipid- and protein-containing particles and consists of two major apolipoproteins apoA-I and apoA-II. ApoA-II has been shown to be involved in the pathogenesis of insulin resistance, adiposity, diabetes, and metabolic syndrome. In embryo, apoa2 mRNAs are abundant in the liver, brain, lung, placenta, and in fish yolk syncytial layer (YSL), suggesting that apoa2 may perform a function during embryonic development. Here we find out that apoa2 modulates zebrafish embryonic development by regulating the organization of YSL. Disruption of apoa2 function in zebrafish caused chromosome fusing, which strongly blocked YSL nuclear division, inducing disorders in YSL organization and finally disturbing the embryonic epiboly. Purified native human apoA-II was able specifically to rescue the defects and induced nuclear division in zebrafish embryos and in human HeLa cells. The C terminus of apoA-II was required for the proper chromosome separation during nuclear division of YSL in zebrafish embryos and in human HeLa cells. Our data indicate that organization of YSL is required for blastoderm patterning and morphogenesis and suggest that apolipoprotein apoA-II is a novel factor of nuclear division in YSL involved in the regulation of early zebrafish embryonic morphogenesis and in mammalian cells for proliferation.
authors.affiliation: Laboratory of Stem Cell Biology, West China Hospital West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Yao
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Chaoran
authors.initials: C
authors.lastname: Yin
authors.forename: Chun
authors.initials: C
authors.lastname: Xiao
authors.forename: Meilin
authors.initials: M
authors.lastname: Qian
authors.forename: Donghui
authors.initials: D
authors.lastname: Liu
authors.forename: Lemin
authors.initials: L
authors.lastname: Zheng
authors.forename: Wentong
authors.initials: W
authors.lastname: Meng
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhu
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Xianming
authors.initials: X
authors.lastname: Mo
mesh_terms: D000818:Animals; D016633:Apolipoprotein A-II / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D001756:Blastoderm / Q000166:cytology / Q000378:metabolism*; D019521:Body Patterning / Q000187:drug effects / Q000502:physiology*; D048750:Cell Nucleus Division / Q000187:drug effects / Q000502:physiology*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D015726:Giant Cells / Q000166:cytology / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009024:Morphogenesis / Q000187:drug effects / Q000502:physiology*; D015027:Zebrafish
chemical_list: D016633:Apolipoprotein A-II