title: Separation of 9,10-anthraquinone derivatives: evaluation of C18 stationary phases.
abstract: Chromatographic study of natural products helps to determine their molecular composition and to identify their sources (biological, geographical, etc.). However, identifying anthraquinoids is still a challenge because this chemical family is composed of more than half a thousand molecules. In the present work, a series of C18 stationary phases were systematically studied to evaluate their separation potential for these compounds in high performance reversed phase liquid chromatography (RPLC). The stationary phases with various physicochemical properties were evaluated with respect to the separation of 30 standards. Selectivity of the studied stationary phases and symmetry of the obtained peaks were chosen as the evaluation criteria. Calculations of symmetry and separation capacity parameters have been proposed. These parameters are not dependent on the dimensions of the columns and were used for the classifications of phases. In conclusion, this study is intended to be a guide as to which stationary phases analysts can use for resolving separation of a complex mixture of anthraquinoids.
authors.affiliation: Univ. Paris-Sud, Groupe de Chimie Analytique de Paris-Sud EA 4041-LETIAM-IUT d'Orsay, Plateau du Moulon, Orsay F 91400, France. myriam.bonose@u-psud.fr
authors.forename: Myriam
authors.initials: M
authors.lastname: Bonose-Crosnier de Bellaistre
authors.forename: Witold
authors.initials: W
authors.lastname: Nowik
authors.forename: Alain
authors.initials: A
authors.lastname: Tchapla
authors.forename: Sylvie
authors.initials: S
authors.lastname: Heron
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000302:isolation & purification; D008956:Models, Chemical; D008968:Molecular Conformation
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; C042834:9,10-anthraquinone