title: [Proteomic study of retinoid acid resistant NB4R1 cells apoptosis induced by realgar].
abstract: OBJECTIVE
  To obtain and identify the differential proteome of apoptosis induced by realgar (tetra-arsenic tetra-sulfide, As(4)S(4)) in retinoid acid (RA) resistant human acute promyelocytic leukemia (APL) cell line NB4-R1 cells.
  
  
  METHODS
  The comparative proteomic expressive profiles of NB4-R1 cells treated with and without As(4)S(4) were obtained with the high-resolution two-dimensional electrophoresis system. After the analysis of ImageMaster(TM) 2D Platinum software combining artificial comparison. The differential expression proteins were screened and performed in-gel digestion and extraction of peptides, applied mass spectrometry MALDI-TOF-MS, MALDI-TOF/TOF, UPLC-MS/MS and bioinformatics to identify differential expressive proteins.
  
  
  RESULTS
  Twenty-two spots with more than 2-fold (≥ 2 or ≤ 0.5) expression changes were identified and 21 known proteins obtained, including 5 down-regulated (SET/I2PP2A, RPP2, PCBP1, EIF4H-1 and ANP32A/I1PP2A) after exposed to As(4)S(4), 2 up-regulated (HSP27 and HMGB1) after exposed for 24 h, and 14 up-regulated (PHB, ERP29, DNAJC8, PSMB4, ACTB, RPP0, RhoGDI2, alpha-tubulin, transcription factor, RBM15/OTT1, eIF5A1 and H2B1M et al.) after exposed for 48 h.
  
  
  CONCLUSIONS
  It is the first time to successfully obtain and identify the global proteome of apoptosis induced by As(4)S(4) in RA-resistant cells. Among them, expressional and functional regulation of target proteins SET, RPP2 and PHB might be the potential novel therapeutic target for RA-resistant APL.
authors.affiliation: Department of Hematology, the First Clinical Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: He
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Wang
authors.forename: Xin-Yang
authors.initials: XY
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy; D000091402:Prohibitins; D020543:Proteome; D040901:Proteomics; D012176:Retinoids; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014212:Tretinoin / Q000494:pharmacology
chemical_list: C000717328:PHB protein, human; D000091402:Prohibitins; D020543:Proteome; D012176:Retinoids; D014212:Tretinoin