title: [Molecular genetic features of sporadic Burkitt's lymphoma in children].
abstract: OBJECTIVE
  To investigate the molecular genetic features and diagnostic aspects of sporadic Burkitt's lymphoma (BL) in children.
  
  
  METHODS
  Tissue microarray was constructed to include 64 cases of pediatric BL and 6 cases of pediatric diffuse large B-cell lymphoma (DLBCL). Immunohistochemistry and fluorescence in-situ hybridization for c-myc, bcl-2, bcl-6, IgH, myc/IgH and bcl-2/IgH gene were performed. Cases of pediatric Burkitt's lymphomas were subclassified into three groups based on their cellular orgins: the germinal center (GC) group, the late-germinal center (late-GC) group and the post-germinal center (post-GC) group.
  
  
  RESULTS
  Among 64 Burkitt's lymphomas studied, expression of CD20, CD10, bcl-6, bcl-2 and MUM1 by immunohistochemistry were 100% (64 cases), 98.4% (63 cases), 96.9% (62 cases), 0 (0 cases) and 23.4% (15 cases), respectively. Various gene rearrangements were found involving the c-myc 93.1% (54/58 cases) and IgH 82.8% (48/58 cases). Detailed rearrangements are as follows: 46 cases (85.2%) myc/IgH gene translocation along with c-myc and IgH gene rearrangement; 4 cases (7.4%) c-myc gene rearrangement without IgH and myc/IgH abnormality; 4 cases (7.4%) without c-myc, IgH or myc/IgH gene rearrangement. No case showed bcl-2 gene abnormality (100%). Fifty nine cases showed normal bcl-6 gene status. One case had bcl-6 gene rearrangement and amplification with the pathologic and immunophenotypic characteristics of BL, leading to a revised pathological diagnosis of B-cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma and Burkitt's lymphoma (DLBCL/BL). Two cases showed c-myc gene rearrangement. Two cases showed bcl-6 gene amplification and 6 DLBCL cases had a normal status of bcl-2/IgH.
  
  
  CONCLUSIONS
  A majority of pediatric sporadic BL arise from the germinal center B cells, most of which have c-myc gene rearrangement. It is useful to distinguish BL and DLBCL by multiple genes detection.
authors.affiliation: Department of Pathology, Jiangxi Children's Hospital, Nanchang 330006, China. ywp07912000@yahoo.com.cn
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Gong
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Xu
authors.forename: Yin
authors.initials: Y
authors.lastname: Zou
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Lü
authors.forename: Hua-Sheng
authors.initials: HS
authors.lastname: Zhong
authors.forename: Qing-Qiang
authors.initials: QQ
authors.lastname: Deng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiao
authors.forename: Song-Tao
authors.initials: ST
authors.lastname: Zeng
authors.forename: Cai-di
authors.initials: CD
authors.lastname: Zhu
mesh_terms: D018951:Antigens, CD20 / Q000378:metabolism*; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D015260:Neprilysin / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000378:metabolism; D014178:Translocation, Genetic
chemical_list: D018951:Antigens, CD20; D007143:Immunoglobulin Heavy Chains; D019253:Proto-Oncogene Proteins c-bcl-2; D051560:Proto-Oncogene Proteins c-bcl-6; D015260:Neprilysin