title: Absence of hemolytic disease of fetus and newborn despite maternal high-titer IgG anti-Ku.
abstract: Anti-Ku seen in K(o) (Kell-null) individuals has previously been shown to cause severe hemolytic transfusion reactions. Maternal anti-Ku can cause none or moderate to severe hemolytic disease of the fetus and newborn (HDFN). In two of four previously described HDFN cases, intrauterine transfusions were required because of severe anemia. We report a case in which maternal anti-Ku did not cause HDFN. Standard serologic methods were used for RBC antibody screening and identification, adsorption and elution of RBC antibodies, and antigen typing. A gravida 3, para 3 (G3P3) woman was first evaluated in 2006 and was found to have an IgG RBC antibody that reacted against all panel RBCs in the anti-human globulin phase. A panel of RBCs treated with DTT did not react with the antibody. The antibody failed to react with one example of K(o) RBCs. The patient’s RBCs typed negative for the following Kell blood group antigens: KEL1, KEL2, KEL3, KEL4, KEL6, KEL7, KEL11, KEL13, and KEL18. These results established the presence of anti-Ku in maternal serum. The newborn was group A, D+ and required phototherapy for hyperbilirubinemia, but did not require transfusion. The woman was seen again in January 2010 during the third trimester (G4P3). At this time, anti-Ku titer was 256. She delivered a healthy group O, D+ baby boy at 37 weeks' gestation. Cord RBCs were 4+ for IgG by DAT. An eluate reacted with all RBCs tested, but did not react when tested against a panel of DTT-treated RBCs. K(o) phenotype is rare to begin with, and the maternal anti-Ku formation may require more than one pregnancy. Therefore, cases that can be evaluated for anti-Ku–related HDFN are rare. Our case contributes to serologic and clinical aspects of such rare cases.
authors.affiliation: LifeSource, 1205 North Milwaukee Avenue, Glenview, IL 60025, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Kakaiya
authors.forename: A
authors.initials: A
authors.lastname: Whaley
authors.forename: C
authors.initials: C
authors.lastname: Howard-Menk
authors.forename: J
authors.initials: J
authors.lastname: Rami
authors.forename: M
authors.initials: M
authors.lastname: Papari
authors.forename: S
authors.initials: S
authors.lastname: Campbell-Lee
authors.forename: Z
authors.initials: Z
authors.lastname: Malecki
mesh_terms: D034961:Antigens, Nuclear / Q000097:blood / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000097:blood / Q000276:immunology*; D004899:Erythroblastosis, Fetal / Q000209:etiology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007231:Infant, Newborn; D007626:Kell Blood-Group System / Q000032:analysis; D000072200:Ku Autoantigen; D008297:Male; D011247:Pregnancy; D012680:Sensitivity and Specificity
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D007626:Kell Blood-Group System; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen