title: Multicolor Brainbow imaging in zebrafish.
abstract: This protocol describes how to use the Brainbow strategy to label neurons in many different hues. The Brainbow system uses a random Cre/lox recombination to create varied combinations of red, blue, and green fluorescent proteins in each cell. The differences in color allow users to follow multiple cells, regardless of how closely they are positioned. This protocol describes how to use Brainbow imaging in zebrafish and provides examples of how to use color as a guide to trace axonal processes. We use the zebrafish trigeminal sensory ganglion as an example and discuss potential modifications for the general use of this technique.
authors.forename: Y Albert
authors.initials: YA
authors.lastname: Pan
authors.forename: Jean
authors.initials: J
authors.lastname: Livet
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Sanes
authors.forename: Jeff W
authors.initials: JW
authors.lastname: Lichtman
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Schier
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology*
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins