title: Improved rapid detection of trypsin isoinhibitors using non-denaturing polyacrylamide gels with immobilised azoalbumin.
abstract: BACKGROUND
  Proteinaceous inhibitors of animal trypsin occur naturally as isoforms in seeds and some are of interest as antinutritional or anti-pest agents.
  
  
  OBJECTIVE
  To establish a simplified electrophorectic, in-gel method for rapid and direct detection of trypsin isoinhibitors present in crude plant extracts that are particularly suitable for many studies including rapid evaluation of cultivars.
  
  
  METHODS
  Azoalbumin (3%, w/v) is immobilised in 7.5% polyacrylamide gels before electrophoresis under non-denaturing conditions.
  
  
  RESULTS
  This improved method eliminates the need for both time-consuming and labourious staining and destaining or renaturation steps.
  
  
  CONCLUSIONS
  Immobilised azoalbumin in polyacrylamide gels, run under non-denaturing electrophoresis conditions, can be used to assist rapid evaluation of trypsin isoinhibitors in numerous crude plant extracts.
authors.affiliation: School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand.
authors.forename: Hossein
authors.initials: H
authors.lastname: Alizadeh
authors.forename: David W M
authors.initials: DW
authors.lastname: Leung
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D014361:Trypsin Inhibitors / Q000032:analysis* / Q000737:chemistry
chemical_list: D000418:Albumins; D055767:Immobilized Proteins; D010936:Plant Extracts; D014361:Trypsin Inhibitors; C031460:azoalbumin