title: DEXH-Box protein DHX30 is required for optimal function of the zinc-finger antiviral protein.
abstract: The zinc-finger antiviral protein (ZAP) is a host factor that specifically inhibits the replication of certain viruses by eliminating viral mRNAs in the cytoplasm. In previous studies, we demonstrated that ZAP directly binds to the viral mRNAs and recruits the RNA exosome to degrade the target RNA. In this article, we provide evidence that a DEXH box RNA helicase, DHX30, is required for optimal antiviral activity of ZAP. Pull-down and co-immunoprecipitation assays demonstrated that DHX30 and ZAP interacted with each other via their N terminal domains. Downregulation of DHX30 with shRNAs reduced ZAP's antiviral activity. These data implicate that DHX30 is a cellular factor involved in the antiviral function of ZAP.
authors.affiliation: Center for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Peiying
authors.initials: P
authors.lastname: Ye
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Guifang
authors.initials: G
authors.lastname: Chen
authors.forename: Guangxia
authors.initials: G
authors.lastname: Gao
mesh_terms: D003593:Cytoplasm / Q000378:metabolism / Q000502:physiology; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding / Q000502:physiology; D012313:RNA / Q000378:metabolism / Q000502:physiology; D020365:RNA Helicases / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism / Q000502:physiology; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; C527496:ZC3HAV1 protein, human; D012313:RNA; C558578:DHX30 protein, human; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases