title: Upregulation of interferon-gamma-induced genes during prion infection.
abstract: Global gene expression analysis allows for the identification of transcripts that are differentially regulated during a disease state. Many groups, including our own, have identified hundreds of genes differentially regulated in response to prion infection. Eleven transcripts, upregulated in the brains of prion-infected animals, which were classified in the literature as stimulated by the cytokine interferon-gamma (IFN-γ), were identified. This is intriguing, as IFN-γ has recently been detected in the brains of prion-infected animals. Quantitation of several genes, categorized as IFN-γ inducible, by quantitative real-time polymerase chain reaction (qRT-PCR) confirms that these transcripts are upregulated. Future approaches for delineating the role of IFN-γ-induced transcripts and their function in prion infection are described.
authors.affiliation: Cellular and Molecular Biology Graduate Program, Department of Comparative Biosciences, University of Wisconsin, Madison, WI, USA.
authors.forename: Laura R
authors.initials: LR
authors.lastname: Moody
authors.forename: Allen J
authors.initials: AJ
authors.lastname: Herbst
authors.forename: Judd M
authors.initials: JM
authors.lastname: Aiken
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D017096:Prion Diseases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma