title: [Determination of plasma protein binding rate of tetramethylpyrazine phosphate by ultrafiltration].
abstract: OBJECTIVE
  To study the plasma protein binding rate of Tetramethylpyrazine Phosphate.
  
  
  METHODS
  The ultrafiltration was employed to determine the plasma protein binding rate of Tetramethylpyrazine Phosphate. The plasma concentrations of Tetramethylpyrazine Phosphate were measured by RP-HPLC.
  
  
  RESULTS
  The plasma protein binding rate of Tetramethylpyrazine Phosphate with HSA, human plasma and rat plasma were (64.64 +/- 0.68)%, (65.85 +/- 7.35)% and (73.65 +/- 2.35)%, respectively.
  
  
  CONCLUSIONS
  The binding rate of Tetramethylpyrazine Phosphate with plasma protein is middling strength.
authors.affiliation: Guangzhou University of Chinese Medicine, China. yong-ye@163.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.forename: Li-ling
authors.initials: LL
authors.lastname: Zhou
authors.forename: Yi-lin
authors.initials: YL
authors.lastname: Yan
authors.forename: Qiu-jie
authors.initials: QJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010710:Phosphates / Q000378:metabolism*; D011485:Protein Binding; D011719:Pyrazines / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods*; D014665:Vasodilator Agents / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D010710:Phosphates; D011719:Pyrazines; D014665:Vasodilator Agents; C017953:tetramethylpyrazine