title: Crystallization and preliminary crystallographic characterization of three peptidic inhibitors in complex with α-thrombin.
abstract: The serine protease thrombin plays a major role in thrombosis and haemostasis. This has driven interest in thrombin inhibitors as potential antithrombotic drugs. Here, the crystallization and preliminary crystallographic analysis of human α-thrombin in complex with three noncovalent peptide inhibitors of the general sequence D-Phe-Pro-D-Arg-P1'-CONH2 are reported. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1) and diffracted to beyond 1.3 Å resolution.
authors.affiliation: IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Carvalho Figueiredo
authors.forename: Cristina C
authors.initials: CC
authors.lastname: Clement
authors.forename: Manfred
authors.initials: M
authors.lastname: Philipp
authors.forename: Pedro José
authors.initials: PJ
authors.lastname: Barbosa Pereira
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*; D013917:Thrombin / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000235:genetics
chemical_list: D010455:Peptides; D015842:Serine Proteinase Inhibitors; D013917:Thrombin