title: [The effect of platelet factor 4 on the chemoattract function of the cord blood CD34+ cells].
abstract: OBJECTIVE
  To study the effect of PF4 and relative peptide PF4 17-70 on the chemoattract ability, the expression of adhesion molecules and CXCR4 on the flesh cord blood CD34+ cells.
  
  
  METHODS
  CD34+ cells were separated from the cord blood using MACS immune magnetic beads, the chemoattract ability was assayed using the Transwell board, the expression of adhesion molecules and CXCR4 was measured by FACS.
  
  
  RESULTS
  (1) PF4 and PF4 17-70 increased the migration of the CD34+ cells, the chemoattract percentage of PF4 was 157.43% +/- 50.06% (P < 0.05) and PF4 17-70 was 187.02% +/- 10.69% (P < 0.05). (2) The expression of CD49d and CXCR4 on the CD34+ cells increased after PF4 incubated, but the expressions of other adherent molecules including CD31, CD44, CD11a, CD62p, CD62E did not change.
  
  
  CONCLUSIONS
  PF4 has the chemoattract ability on the umbilical blood CD34+ cells by promoting the expression of integrin CD49d and CXCR4, PF4 may help the cord stem cells homing.
authors.affiliation: State key Laboratory of Experimental Hematology, Institute of Hematology, National Research Center of Stem Cell Engineering & Technology, CAMS & PUMC, Tianjin 300020, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: Lu
authors.forename: Guo-li
authors.initials: GL
authors.lastname: Song
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Zhong-chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects*; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D039441:Integrin alpha4 / Q000378:metabolism; D010978:Platelet Factor 4 / Q000494:pharmacology*; D019718:Receptors, CXCR4 / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; C527276:CXCR4 protein, human; D015815:Cell Adhesion Molecules; D019718:Receptors, CXCR4; D039441:Integrin alpha4; D010978:Platelet Factor 4