title: Genetic evidence that DNA methyltransferase DRM2 has a direct catalytic role in RNA-directed DNA methylation in Arabidopsis thaliana.
abstract: RNA-directed DNA methylation (RdDM) is a small RNA-mediated epigenetic modification in plants. We report here the identification of DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) in a forward screen for mutants defective in RdDM in Arabidopsis thaliana. The finding of a mutation in the presumptive active site argues in favor of direct catalytic activity for DRM2.
authors.affiliation: Gregor Mendel Institute, Austrian Academy of Sciences, A-1030 Vienna, Austria.
authors.forename: Ulf
authors.initials: U
authors.lastname: Naumann
authors.forename: Lucia
authors.initials: L
authors.lastname: Daxinger
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kanno
authors.forename: Changho
authors.initials: C
authors.lastname: Eun
authors.forename: Quan
authors.initials: Q
authors.lastname: Long
authors.forename: Zdravko J
authors.initials: ZJ
authors.lastname: Lorkovic
authors.forename: Marjori
authors.initials: M
authors.lastname: Matzke
authors.forename: Antonius J M
authors.initials: AJ
authors.lastname: Matzke
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D017354:Point Mutation; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D012313:RNA; D008780:Methyltransferases; C470732:DRM2 protein, Arabidopsis