title: Integration of ground aerogel particles as chromatographic stationary phase into microchip.
abstract: C16 modified and ground monolithic silica aerogel particles in submicrometer size, as a new type of stationary phase was prepared and integrated in polydimethylsiloxane (PDMS) microchip. The aerogel particles were packed into the microfluidic channel using a simple procedure, which does not require any special frit or fabrication step to retain the particles. The subnanoliter volume of samples can be transported through the porous, short length of packing with low pressure (< 3 bar). Food dyes as test components could be separated using low pressure within 6s. A 50-fold preconcentration could be achieved by retaining 100 nL volume of sample on the packing and elution with methanol.
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Debrecen, P.O. Box 21, H-4010 Debrecen, Hungary. gaspara@tigris.unideb.hu
authors.forename: Attila
authors.initials: A
authors.lastname: Gaspar
authors.forename: Andrea
authors.initials: A
authors.lastname: Nagy
authors.forename: Istvan
authors.initials: I
authors.lastname: Lazar
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation*; D004396:Coloring Agents / Q000737:chemistry / Q000302:isolation & purification; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D009757:Nylons / Q000737:chemistry; D010316:Particle Size; D016062:Porosity; D012821:Silanes / Q000737:chemistry*; D058428:Silica Gel / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D004129:Dimethylpolysiloxanes; D009757:Nylons; D012821:Silanes; C085707:poly(dimethylsiloxane)-polyamide copolymer; D058428:Silica Gel; C061205:tetramethoxysilane