title: Structure of the measles virus hemagglutinin bound to its cellular receptor SLAM.
abstract: Measles virus, a major cause of childhood morbidity and mortality worldwide, predominantly infects immune cells using signaling lymphocyte activation molecule (SLAM) as a cellular receptor. Here we present crystal structures of measles virus hemagglutinin (MV-H), the receptor-binding glycoprotein, in complex with SLAM. The MV-H head domain binds to a β-sheet of the membrane-distal ectodomain of SLAM using the side of its β-propeller fold. This is distinct from attachment proteins of other paramyxoviruses that bind receptors using the top of their β-propeller. The structure provides templates for antiviral drug design, an explanation for the effectiveness of the measles virus vaccine, and a model of the homophilic SLAM-SLAM interaction involved in immune modulations. Notably, the crystal structures obtained show two forms of the MV-H-SLAM tetrameric assembly (dimer of dimers), which may have implications for the mechanism of fusion triggering.
authors.affiliation: Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Ose
authors.forename: Marie
authors.initials: M
authors.lastname: Kubota
authors.forename: Nobuo
authors.initials: N
authors.lastname: Maita
authors.forename: Jun
authors.initials: J
authors.lastname: Kamishikiryo
authors.forename: Katsumi
authors.initials: K
authors.lastname: Maenaka
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yanagi
mesh_terms: D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D018360:Crystallography, X-Ray; D006389:Hemagglutinins, Viral / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008457:Measles / Q000821:virology*; D008459:Measles virus / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1
chemical_list: D015703:Antigens, CD; D006389:Hemagglutinins, Viral; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1