title: The first genetic linkage map of Luohanguo (Siraitia grosvenorii ) based on ISSR and SRAP markers.
abstract: In this study, the first genetic map of Luohanguo (Siraitia grosvenorii (Swingle) C. Jeffrey) was constructed with 150 F₂ population individuals using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. A total of 100 ISSRs and 196 SRAP primer combinations generated 51 and 222 polymorphic markers, respectively. Among the 273 markers obtained, 199 markers (29 ISSRs and 170 SRAPs) were mapped to 25 linkage groups. The map covered 1463.3 cM with a mean map distance of 7.35 cM between adjacent markers and a maximum map distance of 52.6 cM between two markers. The markers were distributed randomly in 25 groups except for minor clusters in the distal region of linkage groups. All 25 linkage groups consisted of 2-36 loci ranging in length from 19.5 to 152.6 cM and accounted for 59.8% of the total map distance. This map provides reference information for future molecular breeding work on Luohanguo.
authors.affiliation: Institute of Medicinal Plant Development, Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
authors.forename: Jianhe
authors.initials: J
authors.lastname: Wei
authors.forename: Jiaming
authors.initials: J
authors.lastname: Qin
authors.forename: Changming
authors.initials: C
authors.lastname: Mo
mesh_terms: D001947:Breeding; D002681:China; D002874:Chromosome Mapping; D019662:Cucurbitaceae / Q000235:genetics*; D008040:Genetic Linkage; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic