title: Cellular immunologic response to primary cryoablation of C6 gliomas in rats.
abstract: The immunological consequences of cryoablation for gliomas are largely unknown. cryoablation is an attractive therapeutic option for tumors due to its minimally invasive nature. cryoablation is also potentially immunogenic. With an aim to explore changes in cellular immunity following argon-helium cryosurgery, we established Wistar rat models bearing subcutaneous C6 glioma and divided the rats into the normal control (30 rats), sham-operated (33 rats), surgical resection (30 rats), and cryosurgery (33 rats) groups with corresponding treatments. The tumor cell morphology was observed, and changes in the T lymphocyte subset and NK lymphocyte subset and the ratio of Th1/Th2 were assessed with flow cytometry following the cryosurgery. The results showed that subcutaneous tumor implantation was successful in all cases and this was confirmed histologically. Compared with surgical resection that caused significant reduction in CD3(+), CD4(+), CD14(+), CD16+56 cell percentages, cryosurgery resulted in significantly increased percentages of CD3(+), CD4(+), CD14(+), CD16+56 cells (P < 0.05) with a increase of the Th1/Th2 ratio 7 days after the operation. These results demonstrate that in addition to tumor cell destruction, cryosurgery also results in enhanced cellular immunity, suggesting the great potential of argon-helium cryosurgery in clinical management of gliomas.
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S-Z
authors.initials: SZ
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: Y-W
authors.initials: YW
authors.lastname: Guo
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Ke
authors.forename: X D
authors.initials: XD
authors.lastname: Jiang
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Cai
mesh_terms: D000818:Animals; D003452:Cryosurgery; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology* / Q000601:surgery*; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology; D058408:Th1-Th2 Balance
chemical_list: D016207:Cytokines