title: Primary structure and specificity of a new member of galectin family from the Amethyst deceiver mushroom Laccaria amethystina.
abstract: A new galectin was characterized in the Amethyst deceiver mushroom Laccaria amethystina. The complete amino acid (AA) sequence of the lectin, which exhibited β-galactoside specificity, was deduced from its peptide sequences. The AA sequence of L. amethystina galectin (LAG) showed high homology with those of the same genus, at 75.6% identity to Laccaria bicolor, and 35.5-65.0% to galectins of Agrocybe spp. and Coprinopsis cinerea. The AA sequence of LAG contained all but one conserved residue known to be involved in β-galactoside binding, with His at the position 57 residue replaced by Thr in LAG. Analysis of binding specificity by hemagglutination inhibition assay and enzyme-linked lectin-sorbent assay revealed high specificity of LAG towards O-glycoproteins.
authors.affiliation: Department of Applied Biological Chemistry, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Lyimo
authors.forename: Fumio
authors.initials: F
authors.lastname: Yagi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Minami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001212:Asialoglycoproteins / Q000378:metabolism; D060748:Fetuins; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D037161:Galectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D055399:Laccaria; D008970:Molecular Weight; D011817:Rabbits; D013379:Substrate Specificity; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D001212:Asialoglycoproteins; D060748:Fetuins; D005656:Fungal Proteins; D037161:Galectins; D006023:Glycoproteins; D000509:alpha-Fetoproteins; C017029:asialofetuin