title: Chitosan grafted with macrocyclic polyamines on C-2 and C-6 positions as nonviral gene vectors: preparation, characterization, and in vitro transfection studies.
abstract: Chitosan grafted with macrocyclic polyamines (Cs-g-MCPA) on the C-2 or the C-6 position was synthesized by a simple method. Four copolymers prepared were characterized by (1)H NMR, (13)C NMR, Fourier transform infrared spectra (FTIR), X-ray diffraction (XRD), and gel permeation chromatography (GPC). Circular dichroism spectra (CD), fluorescence spectra and agarose gel electrophoresis assay showed that Cs-g-MCPA copolymers had good binding ability to DNA. By modification of the MCPAs, the copolymers showed low cytotoxicity and high transfection efficiency as new gene vectors. It was found that Cs-g-MCPA copolymers with different grafted positions showed different properties: copolymers grafted on the C-2 position showed higher cytotoxicity and higher transfection efficiency than those grafted on position C-6.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Department of Pharmaceutical Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.forename: Na
authors.initials: N
authors.lastname: Rong
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Zhu
authors.forename: Renzhong
authors.initials: R
authors.lastname: Qiao
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D047028:Macrocyclic Compounds / Q000138:chemical synthesis* / Q000737:chemistry* / Q000633:toxicity; D015394:Molecular Structure; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection / Q000379:methods*
chemical_list: D047028:Macrocyclic Compounds; D011073:Polyamines; D004247:DNA; D048271:Chitosan; C427203:calf thymus DNA