title: [Establishment of a human large cell lung cancer cell line L9981-nm23-H1].
abstract: BACKGROUND
  To establish a human large cell lung cancer cell line L9981-nm23-H1 transfected with wild type nm23-H1 gene.
  
  
  METHODS
  pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and L9981-nm23-H1 was established by infected virus of nm23-H1 gene. The DNA and protein expression of nm23-H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot.
  
  
  RESULTS
  pLXSN-nm23-H1-EGFP was constructed successfully, and the nm23-H1 cDNA was inducted to L9981 cell. The protein of nm23-H1 could be detected in L9981-nm23-H1 cell.
  
  
  CONCLUSIONS
  Protein of nm23-H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.
authors.affiliation: The Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
authors.forename: Liangan
authors.initials: L
authors.lastname: Che
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Lunxu
authors.initials: L
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Sun
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu