title: [Experimental study on monitoring gene expression by noninvasive method in rabbit VX2 liver tumor model].
abstract: OBJECTIVE
  To investigate the feasibility of monitoring therapeutic effect of adenovirus vector containing IL12-IRES-CKb gene on a rabbit VX2 liver tumor model by using phosphorous-31 magnetic resonance spectroscopy (31P MRS).
  
  
  METHODS
  A total of 18 healthy New Zealand White rabbits were used to generate animal models by implanting VX2 tumor chips into livers through laparotomy. Tumor-bearing animals were randomly divided into three groups and were injected with AdCMVIL12-IRES-CKb, AdCMV-Empty and saline respectively via ear veins. 31P MRS scan was performed after animals were fed with creatine solution for five days. Animals were euthanized thereafter and tumors were removed for pathological examination, immunohistochemistry (IHC) staining and protein analysis (Western blot).
  
  
  RESULTS
  The intrahepatic and seral expressions of creatine kinase (CKb) and IL-12 were detected only in AdCMVIL12-IRES-CKb group. Tumor diameters pre- and post- treatment in three groups were 1.63+/-0.04 vs 1.62+/-0.03 in AdCMVIL12-IRES-CKb group (P = 0.229), 1.59+/-0.05 vs 1.84+/-0.11 in AdCMV-Empty group (P = 0.003) and 1.60+/-0.02 vs 2.07+/-0.12 in saline group (P = 0.001), respectively. Pcr Changes between pre- and post- treatment among the three groups were compared (F = 6.235, P value is less than 0.05). PCr increased significantly in AdCMVIL12-IRES-CKb group as compared to AdCMV-Empty (P = 0.004) and saline group (P = 0.049), whereas no change found between AdCMV-Empty and saline group (P = 0.153).
  
  
  CONCLUSIONS
  31P MRS, an effective and non-invasive functional imaging method, can be used to monitor the therapeutic effect of adenovirus vector containing IL12-IRES-CKb gene on rabbit VX2 liver tumor model through detecting metabolic product of imaging reporter gene CKb (pCr).
authors.affiliation: Department of Gastroenterology, Guangdong Geriatrics Institute, Guangzhou 510080, China. zijunli2005@yahoo.com.cn
authors.forename: Zi-jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Ma
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Shao-qing
authors.initials: SQ
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Bao-peng
authors.initials: BP
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Jin-yong
authors.initials: JY
authors.lastname: Yang
authors.forename: Yu-bao
authors.initials: YB
authors.lastname: Liu
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Liang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D003402:Creatine Kinase / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D018664:Interleukin-12 / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits
chemical_list: D018664:Interleukin-12; D003402:Creatine Kinase