title: [Expression of estrogen receptors alpha and beta in human tongue squamous cancer cell and influence of beta-estradiol on the proliferation of tongue cancer cell].
abstract: OBJECTIVE
  To observe the expression of estrogen receptors alpha and beta in human tongue squamous cancer line Tca8113 cell, and to study the influence of beta-estradiol (beta-E2) on the proliferation and cell cycle of cultured Tca8113 cell.
  
  
  METHODS
  Immunocytochemistry and RT-PCR methods were used to observe the expression of estrogen receptors (ER) in human tongue squamous carcinoma line Tca8113 cell. 3H-TdR incorporation and cell cycle analysis were used to examine the change of proliferation and DNA synthesis of Tca8113 cell.
  
  
  RESULTS
  ER-alpha and ER-beta mRNA were expressed in human tongue squamous cancer cell, and the expression of ER-beta was weaker than that of ER-alpha. beta-Estradiol at 10(-8) mol/L - 10(-6) mol/L could increase the proliferation of human tongue squamous carcinoma cell in a dose dependent manner (P < 0.01). beta-E2 (10(-6) mol/L) could increase the proportion of cells in S phase and G2 phase from 23.5% up to 37.7%. The effect of estradiol on the proliferation of cultured human tongue squamous cancer line Tca8113 cell could be inhibited by Tamoxifen.
  
  
  CONCLUSIONS
  There are ER-alpha and ER-beta expression in human tongue squamous cancer line Tca8113 cell, and beta-estradiol promotes the proliferation and cell cycle of cultured human Tca8113 cell.
authors.affiliation: Department of Physiology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Liu
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Chi
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Zhu
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Zhong
authors.forename: Cai-Fang
authors.initials: CF
authors.lastname: Xue
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000378:metabolism*; D006801:Humans; D013629:Tamoxifen / Q000494:pharmacology; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D013629:Tamoxifen; D004958:Estradiol