title: Vaginal reconstruction using no grafts with evidence of squamous epithelialization in neovaginal vault: a simple approach.
abstract: OBJECTIVE
  To present a simple approach for vaginal reconstruction, without lining of the neovagina by means of any grafts.
  
  
  METHODS
  This was an observational prospective cohort study. Twenty-five patients with a known diagnosis of vaginal agenesis were enrolled in this study between 2003 and 2009. All patients underwent vaginoplasty in which the McIndoe procedure was used with no grafts. Then we assessed vaginal depth, quality of life, sexual function, self-image and surgical complications with an interview, examination and by filling in the standard questionnaires in regards to the outcome of the surgery. Moreover, punch biopsy was taken from the neovagina at 3-12 months after surgery and the histology of samples was evaluated to determine squamous epithelialization of the neovagina.
  
  
  RESULTS
  Most of the patients in our study were satisfied with the outcome of surgery due to few complications. Moreover, punch biopsy after vaginal reconstruction determined complete epithelialization of the neovagina in six out of seven patients.
  
  
  CONCLUSIONS
  The procedure described here offers patients a near-normal vagina with a simple, less invasive, low-cost procedure that provides squamous epithelialization of the neovaginal vault.
authors.affiliation: Departments of Obstetrics and GynecologyPathologyAnesthesiology, Mirza Koochak Khan HospitalSchool of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ghafarin2@yahoo.com
authors.forename: Ghafarnejad
authors.initials: G
authors.lastname: Marzieh
authors.forename: Darvish
authors.initials: D
authors.lastname: Soodabeh
authors.forename: Izadi-Mood
authors.initials: IM
authors.lastname: Narges
authors.forename: Samimi-Sade
authors.initials: SS
authors.lastname: Saghar
authors.forename: Esmaeili
authors.initials: E
authors.lastname: Sara
mesh_terms: D000293:Adolescent; D000328:Adult; D015331:Cohort Studies; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D006801:Humans; D011446:Prospective Studies; D011788:Quality of Life; D019651:Plastic Surgery Procedures / Q000009:adverse effects / Q000379:methods* / Q000523:psychology; D012649:Self Concept; D016038:Skin Transplantation / Q000009:adverse effects; D016896:Treatment Outcome; D014621:Vagina / Q000002:abnormalities* / Q000601:surgery*