title: Molecular epidemiology of the 2005 enterovirus 71 outbreak in central Taiwan.
abstract: BACKGROUND
  Since 1998, Taiwan has experienced annual outbreaks of enterovirus 71 (EV71) nationwide. The area around Taichung City experienced a particularly large outbreak in 2005, after which EV71 disappeared for 2 y before re-emerging in 2008. Here we present the clinical, genotypic, and epidemiological baseline data for the 2005 Taichung outbreak.
  
  
  METHODS
  Throat swab, stool and cerebrospinal fluid samples were collected and stored in viral transport medium. Samples were tested by reverse-transcriptase polymerase chain reaction and viral culture. Epidemiological, laboratory, and clinical data were extracted from medical record reviews. A total of 27 virus isolates were selected for phylogenetic analysis.
  
  
  RESULTS
  Confirmed phylogenetic results of the viruses were separated into 5 groups. The 5'-UTR regions served as a focus for investigating genetic relationships among the 27 EV71 isolates, all of which belonged to a distinct clade in the C4 genotype. Most of the strains belonged to 5 observed epidemic groups.
  
  
  CONCLUSIONS
  In conclusion, the 2005 outbreak in central Taiwan was caused by divergent EV71 strains belonging to the C4 genotype.
authors.affiliation: Department of Health Risk Management, China Medical University, Taichung City, Taiwan.
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Lan
authors.forename: Tsai-Hsiu
authors.initials: TH
authors.lastname: Lin
authors.forename: Jeng-Dau
authors.initials: JD
authors.lastname: Tsai
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Yang
authors.forename: Ching-Tien
authors.initials: CT
authors.lastname: Peng
authors.forename: Mu-Chin
authors.initials: MC
authors.lastname: Shih
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Lin
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Lin
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks / Q000706:statistics & numerical data; D029821:Enterovirus A, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000453:epidemiology* / Q000235:genetics; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000097:blood / Q000134:cerebrospinal fluid / Q000453:epidemiology* / Q000235:genetics; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology / Q000706:statistics & numerical data; D008969:Molecular Sequence Data; D010614:Pharynx / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013624:Taiwan / Q000453:epidemiology; D014709:Vero Cells
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral