title: [Relationship between amyloid beta-protein and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide against oxidative stress induced damage on neuro-2a cells].
abstract: OBJECTIVE
  To observe the relationship between amyloid beta-protein (Abeta) and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide (PACAP, PACAP-27) against damage induced by oxidative stress (H2O2) in neurem-2a cells.
  
  
  METHODS
  With cultured neuro-2a cells the cell survival and apoptosis were measured by MTT assay, Hoechest33258 staining, DNA ladder and the percentage of small DNA fragment.
  
  
  RESULTS
  Concentration-dependent toxicity was induced with H2O2 treatment for 24 h. The neurotoxicity of H2O2 was increased by about 10 times with cotreatment neurons with amyloid beta-protein fragment 25-35 (Abeta(25-35)). While decrease the percentage of small DNA fragmentation the cell survival was increased with co-treatment with PACAP-27(which were added to the culture everyday). The effect of PACAP was not reversed with antagonist of PACAP receptor, PACAP(6-27).
  
  
  CONCLUSIONS
  Abeta and H2O2 can promote each other's neurotoxicity. Cultured neurons were protected by PACAP27 from the neurotoxicity of H2O2 but not through the activation of PACAP-27 receptor.
authors.affiliation: Department of Microbiology, Beijing Military Medical College, Beijing 100071, China. guilanrun@hotmail.com
authors.forename: Lan-Run
authors.initials: LR
authors.lastname: Gui
authors.forename: Bing-Lie
authors.initials: BL
authors.lastname: Zhang
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Fang
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D017209:Apoptosis; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D006861:Hydrogen Peroxide