title: Determination of hippuric acid in human urine by ion chromatography with conductivity detection.
abstract: A simple, rapid, precise and eco-friendly ion chromatography (IC) method for the determination of hippuric acid (HA) in human urine was proposed in this paper. The separation was carried out an anion exchange column with 2.0 mmol L⁻¹ Na₂CO₃ + 2.0 mmol L⁻¹ NaHCO₃ as mobile phase at the flow-rate 0.7 mL min⁻¹. A suppressed conductivity detector was used and the detection limit was 1.0 μg L⁻¹ (S/N=3) for hippuric acid. The analysis time for one run was 30 min under the optimized IC condition. The recovery of hippuric acid was 93.2-98.0% while the relative standard deviation (RSD) was 1.4-2.3% by seven measurements.
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, PR China.
authors.forename: Fuyong
authors.initials: F
authors.lastname: Zhao
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Mingyu
authors.initials: M
authors.lastname: Ding
mesh_terms: D002254:Carbonates; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004553:Electric Conductivity; D005260:Female; D006626:Hippurates / Q000652:urine*; D006801:Humans; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002254:Carbonates; D006626:Hippurates; C005686:sodium carbonate; C030514:hippuric acid