title: Oligosaccharide mass profiling (OLIMP) of cell wall polysaccharides by MALDI-TOF/MS.
abstract: In today's field of plant cell wall research, insights into the structure of wall components are obtained using many different techniques, ranging from spectroscopic and microscopic to chemical and biochemical. In this chapter, we describe one method: oligosaccharide mass profiling (OLIMP). Using OLIMP, we can harness the selective power of a specific wall hydrolase together with the speed and sensitivity of mass spectrometry to provide highly reproducible structural and compositional information about the wall molecule of interest.
authors.affiliation: Energy Biosciences Institute, University of California, Berkeley, CA, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Günl
authors.forename: Florian
authors.initials: F
authors.lastname: Kraemer
authors.forename: Markus
authors.initials: M
authors.lastname: Pauly
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000166:cytology; D002473:Cell Wall / Q000737:chemistry*; D006056:Golgi Apparatus / Q000737:chemistry; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry / Q000166:cytology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides