title: [Regulation of nm23-H1 transfection on expression of integrins in human high-metastasis large cell lung cancer cell line L9981].
abstract: BACKGROUND
  To study the regulation of nm23-H1 gene on the expression of integrins in human high-metastasis large cell lung cancer cell line L9981 by transfecting nm23-H1 into cell.
  
  
  METHODS
  Lipofect was used to transfect nm23-H1 into the L9981 cell line; semi-RT-PCR was used to detect the difference of integrin β1 and integrin β3 mRNA expressions between tranfected and non-transfected cell lines; flow cytometry was used to detect the difference of integrin β1 and integrin β3 protein expressions between transfected and non-transfected cell lines.
  
  
  RESULTS
  (1) A transgenic cell line L9981-nm23-H1 was obtained, which could stably and effectively express nm23-H1. The mRNA expressions of integrin β1 and β3 in L9981-nm23-H1 cell line were remarkably lower than those in L9981 cell line. (2) The protein expressions of integrin β1 and β3 in L9981-nm23-H1 cell line were significantly lower than those in L9981 cell line too (P < 0.01).
  
  
  CONCLUSIONS
  Transfection of nm23-H1 gene into the L9981 cell line can significantly down-regulate the mRNA and protein expressions of integrin β1 and β3 in this cell line, which indicates that nm23-H1 may reverse the metastasis potential of L9981 cell line through modulation of integrin expression.
authors.affiliation: Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zheng
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Qin
authors.forename: Wen
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Zefang
authors.initials: Z
authors.lastname: Sun
authors.forename: Guowei
authors.initials: G
authors.lastname: Che