title: [Study of calcium buffer in SH-SY5Y cells].
abstract: OBJECTIVE
  To study the calcium buffer in SH-SY5Y cells.
  
  
  METHODS
  By using patch clamp technique, measure voltage-gated calcium currents in undifferentiated SH-SY5Y cell line. Then, by using microfluorometric technique, detect the intracellular [Ca2+]i and the dynamic recovery course after high-K+ induced [Ca2+]i elevation.
  
  
  RESULTS
  There are voltage-gated calcium currents in undifferentiated SH-SYSY cell line. When the time intervals between the stimulus are < 150s, the recovery course will be much more deferred because of the saturation of the intracellular calcium buffer; and when the intervals between the stimulus are > 150 s, the buffer will recruit so as to lead to the steadiness state of the recover course.
  
  
  CONCLUSIONS
  Calcium buffer proteins have an important effect in the course of cellular calcium signal transduction.
authors.affiliation: Institute of Biophys and Biochem, Huazhong Univ of Sci & Tech, Wuhan 430074, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Ye
authors.forename: L Y
authors.initials: LY
authors.lastname: Chen
authors.forename: A L
authors.initials: AL
authors.lastname: Qu
authors.forename: T
authors.initials: T
authors.lastname: Xu
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D020013:Calcium Signaling; D045744:Cell Line, Tumor; D004594:Electrophysiology; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D018408:Patch-Clamp Techniques
chemical_list: D015220:Calcium Channels; D002118:Calcium