title: [Effects of fluoride on ionized calcium and calcium channel in osteoblast-like cell in vitro].
abstract: OBJECTIVE
  To study the effects of fluoride (F-) on ionized calcium ([Ca2+]i) and calcium channel in osteoblast-like cell (OBLs).
  
  
  METHODS
  Investigating [Ca2+]i was by fluorospectrophotometry and recording the calcium currents of OBLs was using whole patch-clamp technique.
  
  
  RESULTS
  The addition of fluoride to the medium made a rapid and significant increase in free [Ca2+]i, especially in 100 ng F- /ml group. There were significances between control group and 50, 100 ng F- /ml groups. And 25 ng/ml F- could stimulate OBLs calcium channel open. Compared with control group, fluoride could significantly increase the amplitudes of calcium currents (P < 0.01), furthermore the activation was in dose-dependent manner.
  
  
  CONCLUSIONS
  Fluoride can make calcium channel open and then increase the concentrations of [Ca2+]i.
authors.affiliation: Department of Pathology, Institute of Biological Engineering, Jilin University, Changchun 130021, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Hua
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: Li
mesh_terms: D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology*; D002460:Cell Line; D005459:Fluorides / Q000633:toxicity*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques
chemical_list: D015220:Calcium Channels; D005459:Fluorides; D002118:Calcium