title: [Study on the Fritillaria cirrhosa seed germination and polyploid induction].
abstract: OBJECTIVE
  In order to improving the existent breeds of Fritillaria cirrhosa, increasing its medicinal ingredients and enriching the resources of heredity breeding.
  
  
  METHODS
  By using of low-temperature stratification treatment, the seed of Fritillaria cirrhosa completed its after-ripening of physiology and morphology. The induction treatment by different concentrations of colchicine solution and different treatment times for Fritillaria cirrhosa seeds were compared.
  
  
  RESULTS
  Detected the plant morphology and chromosome number, it is shown that the induced material obviously possessed the characteristics of polyploid.
  
  
  CONCLUSIONS
  With the treatment of 30 mg/L GA3 for 32 h and stratification for 70 d, the seed germination rate of Fritillaria cirrhosa reached 67.0%. After treated with 0.1% colchicine solution for 48 h, the stratificationed mature seed showed polyploid inductivity of 85.7%.
authors.affiliation: Department of Biological Engineering, Chengdu University, Chengdu 610160, China. wangyaohua888@yahoo.com.cn
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
authors.forename: Sheng-ling
authors.initials: SL
authors.lastname: Hu
authors.forename: Sheng-jie
authors.initials: SJ
authors.lastname: Yan
authors.forename: Zong-sheng
authors.initials: ZS
authors.lastname: He
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Wang
mesh_terms: D003078:Colchicine / Q000494:pharmacology*; D003247:Conservation of Natural Resources / Q000379:methods; D031410:Fritillaria / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D018525:Germination / Q000187:drug effects*; D005875:Gibberellins / Q000494:pharmacology; D010946:Plants, Medicinal / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D011123:Polyploidy; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D012997:Solvents; D013997:Time Factors
chemical_list: D005875:Gibberellins; D012997:Solvents; D003078:Colchicine