title: [Studies on laticifers and milk of greater celandine (Chelidonium majus L.) with fluorescence imaging and fluorescence spectroscopic methods].
abstract: Using fluorescence imaging and fluorescence spectroscopic methods, the localisation of the laticifers and the native spectral properties of the milk were studied in various organs of greater celandine (Chelidonium majus L.). Direct measurements on tissue pieces (without the extraction and the separation of the components) provided information about the complexity of the milk and the various ratios of the alkaloid contents in the tissues. Whole plant were studied in a gel documentation system using ultraviolet light source, while the localisation of the laticifers was observed along the leaf veins in fluorescence microscope, using blue excitation light. Measuring different tissue pieces, fluorescence spectroscopic studies showed that the greater celandine alkaloids have emission bands at 469, 530-531, 553, 572-575 and 592 nm and excitation bands at 365, 370, 386 is 400 nm. These results give a possibility for conclusions about the alkaloid contents and composition or ratios of the alkaloid components in various tissue pieces directly, via comparisons with alkaloid standards.
authors.affiliation: Semmelweis Egyetem, Gyógyszerésztudományi Kar gyógyszerészhallgató, Budapest.
authors.forename: Dorottya
authors.initials: D
authors.lastname: Póczi
authors.forename: Béla
authors.initials: B
authors.lastname: Böddi
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D000818:Animals; D028281:Chelidonium / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry
chemical_list: D000470:Alkaloids