title: Evaluation of the properties of components prepared and stored after holding of whole blood units for 8 and 24 hours at ambient temperature.
abstract: BACKGROUND
  The capability of holding whole blood (WB) units at ambient temperature, overnight, should help in platelet (PLT) concentrate preparation logistics. We summarize the results of a study conducted in the early 1990s that compared, in particular, PLT and red blood cell (RBC) in vivo viability properties following storage after preparation after 8- and 24-hour WB hold periods.
  
  
  METHODS
  Individuals donated units of WB on two occasions. Centrifugation at 20 to 24°C to separate PLTs and additive system RBC placement at 1 to 6°C was completed 8 hours after phlebotomy or after 24 hours in randomized order. Components were not leukoreduced. Studies including in vitro biochemical and hematologic analyses and autologous in vivo RBC and PLT evaluations were conducted at two sites.
  
  
  RESULTS
  RBC 24-hour in vivo (mean ± SD) recoveries (single-label approach), after 35 days of storage, were 79.2 ± 4.3 and 79.4 ± 3.9% (n = 9; p > 0.05), with WB holding periods of 8 and 24 hours, respectively. With 42 days of storage, recovery after a 24-hour hold was slightly less than with an 8-hour hold (72.9 ± 6.5% vs. 76.0 ± 5.4%; n = 17; p < 0.05). RBC 2,3-diphosphoglycerate acid levels were substantially less after the 24-hour hold compared to after the 8-hour hold (n = 18; p < 0.05). PLT recovery after 5 days of storage with 8- and 24-hour hold periods were similar, 51.1 ± 14.9 and 50.6 ± 17.7%, respectively (n = 18; p > 0.05). The PLT survival variable and in vitro properties reflecting storage quality also showed no significant difference.
  
  
  CONCLUSIONS
  RBC and PLT in vivo variables, and most in vitro variables, were not significantly different after storage with WB holding times of 8 and 24 hours except for a slight diminution of RBC recovery with the 24-hour hold after 42 days of storage.
authors.affiliation: American Red Cross, Holland Laboratory, Rockville, MD 20855, USA. johnschris@usa.redcross.org
authors.forename: Gary
authors.initials: G
authors.lastname: Moroff
authors.forename: James P
authors.initials: JP
authors.lastname: AuBuchon
authors.forename: Constance
authors.initials: C
authors.lastname: Pickard
authors.forename: Pamela H
authors.initials: PH
authors.lastname: Whitley
authors.forename: W Andrew
authors.initials: WA
authors.lastname: Heaton
authors.forename: Stein
authors.initials: S
authors.lastname: Holme
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001779:Blood Coagulation Factors / Q000032:analysis; D001781:Blood Component Removal / Q000379:methods*; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D004912:Erythrocytes / Q000737:chemistry; D006461:Hemolysis; D006801:Humans; D015539:Platelet Activation; D016032:Randomized Controlled Trials as Topic / Q000706:statistics & numerical data; D013696:Temperature; D013997:Time Factors
chemical_list: D001779:Blood Coagulation Factors; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate