title: [Abnormal expression of insulin receptor induced by Aβ(1-42) in rat hippocampus neuron].
abstract: OBJECTIVE
  To detect the expression change of insulin receptor under the induction of Aβ(1-42) in rat hippocampus neuron and thus from the receptor level explore the disorder of central nervous insulin signaling and molecular mechanism of Alzheimer's disease.
  
  
  METHODS
  Cultured primary hippocampus neuron was treated with different concentrations of Aβ(1-42). Apoptosis was detected by flow cytometry. And real-time quantitative PCR (polymerase chain reaction) and Western blot were used to detect the expression of insulin receptor.
  
  
  RESULTS
  Primary cultured cells, mature at Day 7, were identified as hippocampal cells. After the treatment with different concentrations of Aβ(1-42) (0 - 150 µmol/L), the ≥ 30 µmol/L treatment groups had greater early apoptosis rates (32.4%, 36.1%, 51.0%, 53.6%) than that in the control group (13.4%) in a concentration-dependent fashion. The PCR results showed that the levels of insulin receptor gene were significantly higher in 30 (2.56 ± 0.19) and 60 µmol/L (3.44 ± 0.23) treatment groups than that the control group (regarded as 1) (P < 0.01) while the 100µmol/L group (0.74 ± 0.15) was significantly lower than the control group (P < 0.01). And the results of Western blot had the same trend with those of PCR. The 30 and 60 µmol/L protein level of the treatment groups were 1.27 ± 0.13, 1.82 ± 0.10 (P < 0.01) and 100µmol/L group was 0.82 ± 0.08 (P < 0.05).
  
  
  CONCLUSIONS
  Aβ(1-42) induces an altered expression of insulin receptors in rat hippocampus cells and results in its functional defects. It may cause insulin resistance in center nervous system.
authors.affiliation: Department of Geriatrics, the Second Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yi-na
authors.initials: YN
authors.lastname: Zhang
authors.forename: Zong-yan
authors.initials: ZY
authors.lastname: Teng
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Jiang
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011972:Receptor, Insulin / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D011972:Receptor, Insulin