title: Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila.
abstract: We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.
authors.affiliation: Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer, The Rockefeller University, New York, NY 10065, USA.
authors.forename: Alexis
authors.initials: A
authors.lastname: Gambis
authors.forename: Pierre
authors.initials: P
authors.lastname: Dourlen
authors.forename: Hermann
authors.initials: H
authors.lastname: Steller
authors.forename: Bertrand
authors.initials: B
authors.lastname: Mollereau
mesh_terms: D000818:Animals; D017209:Apoptosis; D016764:Cell Polarity; D002470:Cell Survival; D004268:DNA-Binding Proteins / Q000502:physiology; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000502:physiology; D005453:Fluorescence; D009154:Mutation; D017956:Photoreceptor Cells, Invertebrate / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C062330:grh protein, Drosophila