title: Salivary interleukin-1β levels in patients with chronic periodontitis before and after periodontal phase I therapy and healthy controls: a case-control study.
abstract: BACKGROUND
  The role of interleukin (IL)-1β in periodontal disease pathogenesis is well researched. This study aimed to assess and compare the salivary IL-1β levels in patients with chronic periodontitis before and after periodontal phase I therapy and periodontally healthy controls. Further, relationships between IL-1β levels and various clinical parameters were explored.
  
  
  METHODS
  Twenty-eight patients with moderate-to-severe generalized chronic periodontitis and 24 age-, race-, and ethnicity-matched controls participated in this study. Saliva samples were obtained from all patients. The clinical parameters recorded were clinical attachment loss (AL), probing depth, bleeding on probing, periodontal index, and gingival index. Clinical evaluation and sample collection were repeated 1 month after periodontal phase I therapy in patients with periodontitis. IL-1β levels were assessed using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Mean IL-1β levels in patients with periodontitis at baseline (1,312.75 pg/mL) were significantly higher (P <0.0001; eight-fold) than in controls (161.51 pg/mL). Although treatment in patients with periodontitis resulted in significant reduction in IL-1β levels (mean: 674.34 pg/mL; P = 0.001), they remained significantly higher (P <0.0001; four-fold) than control levels. There were significant correlations between IL-1β levels and all clinical parameters (P <0.01) except percentage sites with clinical AL >2 mm (P >0.05).
  
  
  CONCLUSIONS
  The data indicate that IL-1β levels are raised in the saliva of patients with chronic periodontitis, which are reduced after phase I therapy, suggesting a close association between salivary IL-1β and periodontitis. Additional longitudinal studies are needed to validate salivary IL-1β as a marker for periodontal disease.
authors.affiliation: Department of Periodontics, Rungta College of Dental Sciences and Research, Bhilai, India. rachnakaushik17@gmail.com
authors.forename: Rachna
authors.initials: R
authors.lastname: Kaushik
authors.forename: Ramreddy K
authors.initials: RK
authors.lastname: Yeltiwar
authors.forename: Kumar
authors.initials: K
authors.lastname: Pushpanshu
mesh_terms: D000328:Adult; D016301:Alveolar Bone Loss / Q000145:classification / Q000276:immunology / Q000628:therapy; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000145:classification / Q000276:immunology* / Q000628:therapy; D012534:Dental Scaling; D005260:Female; D005500:Follow-Up Studies; D005884:Gingival Hemorrhage / Q000145:classification / Q000276:immunology / Q000628:therapy; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009910:Oral Hygiene; D010353:Patient Education as Topic; D017622:Periodontal Attachment Loss / Q000145:classification / Q000276:immunology / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000276:immunology / Q000628:therapy; D010519:Periodontium / Q000276:immunology; D016745:Root Planing; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D012471:Salivary Proteins and Peptides