title: Effect of the microbial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane.
abstract: Microbial lipopeptides play an important role in apoptosis induction of tumor cells. However, there is little knowledge about the relationship between apoptosis induction and membrane fatty acids. The present study focused on the effects of lipopeptides produced by Bacillus subtilis HSO121 on Bcap-37 cell lines. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl (MTT) colorimetric assay and surface tension measurements, showed that the critical micelle concentration (CMC) was a critical level for the inhibitory activity of lipopeptides on the growth of Bcap-37 cells. Under the CMC, the order of least to greatest cytotoxicity effect on cancer cell lines by lipopeptides is C(13)-lipopeptide < C(14)-lipopepitde < C(15)-lipopeptide. Above CMC, all lipopeptides directly exert cytolytic activity. The flow cytometric analysis and Hoechst33258 staining experiments confirmed the apoptosis of Bcap-37 cell lines induced by lipopeptides in a dose-dependent manner. This apoptosis was associated with a significant decrease of the unsaturated degree of the cellular fatty acids of Bcap-37 cell lines due to the changes in the cellular fatty acids composition induced by the lipopeptide treatment. These results indicated that disturbance of the cellular fatty acid composition of breast cancer cell lines were related to in the cell apoptosis. Furthermore, significant difference in IC(50) values of tumor cells and normal cell showed that the lipopeptide exerted selective cytotoxicity on the cancer cells. Thus HSO121 lipopeptides may have potential applications as an anticancer leads.
authors.affiliation: State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Liu
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tao
authors.forename: Aihua
authors.initials: A
authors.lastname: Zou
authors.forename: Shizhong
authors.initials: S
authors.lastname: Yang
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Bozhong
authors.initials: B
authors.lastname: Mu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D001412:Bacillus subtilis; D002453:Cell Cycle; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D055666:Lipopeptides / Q000737:chemistry / Q000494:pharmacology*; D011003:Ploidies; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D005227:Fatty Acids; D055666:Lipopeptides