title: No longitudinal mitochondrial DNA sequence changes in HIV-infected individuals with and without lipoatrophy.
abstract: The potential for mitochondrial (mt) DNA mutation accumulation during antiretroviral therapy (ART), and preferential accumulation in patients with lipoatrophy compared with control participants, remains controversial. We sequenced the entire mitochondrial genome, both before ART and after ART exposure, in 29 human immunodeficiency virus (HIV)-infected Swiss HIV Cohort Study participants initiating a first-line thymidine analogue-containing ART regimen. No accumulation of mtDNA mutations or deletions was detected in 13 participants who developed lipoatrophy or in 16 control participants after significant and comparable ART exposure (median duration, 3.3 and 3.7 years, respectively). In HIV-infected persons, the development of lipoatrophy is unlikely to be associated with accumulation of mtDNA mutations detectable in peripheral blood.
authors.affiliation: Institute of Microbiology, University Hospital Center, and University of Lausanne, Switzerland.
authors.forename: Millán
authors.initials: M
authors.lastname: Ortiz
authors.forename: Estella S
authors.initials: ES
authors.lastname: Poloni
authors.forename: Hansjakob
authors.initials: H
authors.lastname: Furrer
authors.forename: Helen
authors.initials: H
authors.lastname: Kovari
authors.forename: Raquel
authors.initials: R
authors.lastname: Martinez
authors.forename: Mireia
authors.initials: M
authors.lastname: Arnedo
authors.forename: Luigia
authors.initials: L
authors.lastname: Elzi
authors.forename: Enos
authors.initials: E
authors.lastname: Bernasconi
authors.forename: Pietro
authors.initials: P
authors.lastname: Vernazza
authors.forename: Bernard
authors.initials: B
authors.lastname: Hirschel
authors.forename: Matthias
authors.initials: M
authors.lastname: Cavassini
authors.forename: Bruno
authors.initials: B
authors.lastname: Ledergerber
authors.forename: Huldrych F
authors.initials: HF
authors.lastname: Günthard
authors.forename: Amalio
authors.initials: A
authors.lastname: Telenti
authors.forename: Philip E
authors.initials: PE
authors.lastname: Tarr
mesh_terms: D000328:Adult; D044966:Anti-Retroviral Agents / Q000009:adverse effects*; D016022:Case-Control Studies; D015331:Cohort Studies; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D015658:HIV Infections / Q000188:drug therapy / Q000235:genetics*; D039682:HIV-Associated Lipodystrophy Syndrome / Q000188:drug therapy / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D017422:Sequence Analysis, DNA; D013557:Switzerland; D013936:Thymidine / Q000009:adverse effects / Q000031:analogs & derivatives
chemical_list: D044966:Anti-Retroviral Agents; D004272:DNA, Mitochondrial; D013936:Thymidine