title: Bacillus gaemokensis sp. nov., isolated from foreshore tidal flat sediment from the Yellow Sea.
abstract: A Gram-positive, rod-shaped, endospore-forming organism, strain BL3-6(T), was isolated from tidal flat sediments of the Yellow Sea in the region of Tae-An. A 16S rRNA gene sequence analysis demonstrated that this isolate belongs to the Bacillus cereus group, and is closely related to Bacillus mycoides (99.0% similarity), Bacillus thuringiensis (99.0%), Bacillus weihenstephanensis (99.0%), Bacillus cereus (98.9%), Bacillus anthracis (98.8%), and Bacillus pseudomycoides (98.1%). The phylogenetic distance from any validly described Bacillus species outside the Bacillus cereus group was less than 95.6%. The DNA G+C content of the strain was 39.4 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C(14:0) (17.8%), iso-C(16:0) (15.8%), and iso-C(12:0) (11.3%). The diagnostic amino acid of the cell wall was meso-diaminopimelic acid and the major cell wall sugar was galactose. The results of DNA-DNA hybridization (<55.6%) and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain BL3-6(T) from the published Bacillus species. BL3-6(T) therefore represents a new species, for which the name Bacillus gaemokensis sp. nov. is proposed, with the type strain BL3-6(T) (=KCTC 13318(T) =JCM 15801(T)).
authors.affiliation: Korean Collection for Type Cultures, Biological Resource Center, KRIBB, Daejeon, 305-806, Republic of Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Jung
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Jung
authors.forename: Woon Kee
authors.initials: WK
authors.lastname: Paek
authors.forename: In-Soon
authors.initials: IS
authors.lastname: Park
authors.forename: Jeong-Ran
authors.initials: JR
authors.lastname: Han
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Sin
authors.forename: Jayoung
authors.initials: J
authors.lastname: Paek
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Rhee
authors.forename: Hongik
authors.initials: H
authors.lastname: Kim
authors.forename: Hong Seok
authors.initials: HS
authors.lastname: Song
authors.forename: Young-Hyo
authors.initials: YH
authors.lastname: Chang
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D005690:Galactose