title: Synthesis of fluorescent silica nanoparticles and their applications as fluorescence probes.
abstract: This paper presents the synthesis of organic dye molecules embedded silica nanoparticles by Stöber method and their applications as fluorescence probes in cell imaging. By modifying the surface of fluorescent silica nanoparticles (FSNs) with amino, biologically functionalized and monodisperse FSNs can be obtained. In this work, FSNs were conjugated with monoclonal anti-Carcinoembryonic Antigen (anti-CEA) antibody via covalent binding. The antibody-conjugated FSNs can be used to label the SPCA-1 cells successfully, demonstrating that the application of FSNs as fluorescence probes in fluorescence imaging and bioassay would be feasible.
authors.affiliation: Department of Chemistry, Shanghai Normal University, Shanghai 200234, People's Republic of China.
authors.forename: Xu
authors.initials: X
authors.lastname: Song
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Jingwei
authors.initials: J
authors.lastname: Ma
authors.forename: Nengqin
authors.initials: N
authors.lastname: Jia
authors.forename: Jianming
authors.initials: J
authors.lastname: Xu
authors.forename: Hebai
authors.initials: H
authors.lastname: Shen
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D018796:Immunoconjugates; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000138:chemical synthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D012822:Silicon Dioxide