title: Substrate-mediated nucleic acid delivery from self-assembled monolayers.
abstract: Substrate-mediated nucleic acid (NA) delivery involves the immobilization of NAs or NA delivery vehicles to biomaterials for localized transfection of cells. Self-assembled monolayers (SAMs) offer an easy system to immobilize delivery vectors. SAMs form well-defined surfaces; therefore, the effect of surface composition on vector immobilization and transfection efficiency can also be studied. To date, the most effective SAM-mediated delivery systems have utilized nonspecific interactions for immobilization; however, systems that rely on specific interactions between vector and surface can impart higher control of spatial and/or temporal delivery. This review summarizes systems that use both specific and nonspecific interactions for gene delivery from SAMs; highlights progress and remaining challenges; and explores other specific recognition modalities that might be employed for future applications in surface-mediated NA delivery.
authors.affiliation: Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA 98195, USA.
authors.forename: Chung-Huei K
authors.initials: CH
authors.lastname: Wang
authors.forename: Suzie H
authors.initials: SH
authors.lastname: Pun
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018014:Gene Transfer Techniques; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009696:Nucleic Acids / Q000008:administration & dosage* / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D009696:Nucleic Acids