title: Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat.
abstract: Leaf rust resistance gene Lr28 controls one of the important resistances in the Indian subcontinent against the most prevalent Puccinia triticina pathotype 77-5. Pyramiding Lr28 with other resistance genes would therefore, provide durable resistance against rust, a process that can be facilitated by DNA markers. A microsatellite marker wmc313 linked to Lr28 at a distance of 5.0 cM was identified in the population HD2329 × HW2037. The marker was validated in another population developed from WL711× CS + Lr28: 2D/2M 3/8 (acc. 2956) as well as in a few near- isogenic lines (NILs) carrying gene Lr28. Compared to the previously reported marker TPSCAR SCS421(570), wmc313 is more closely linked to Lr28. Both these markers flanking the gene should be useful in the deployment of Lr28 into the breeding program using marker-assisted selection allowing pyramiding with other effective genes to confer durable resistance.
authors.affiliation: Genetics and Plant Breeding Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, India.
authors.forename: Annapurnalilly
authors.initials: A
authors.lastname: Bipinraj
authors.forename: Balgounda
authors.initials: B
authors.lastname: Honrao
authors.forename: Mohinder
authors.initials: M
authors.lastname: Prashar
authors.forename: Subhash
authors.initials: S
authors.lastname: Bhardwaj
authors.forename: Suryaprakasa
authors.initials: S
authors.lastname: Rao
authors.forename: Shubhada
authors.initials: S
authors.lastname: Tamhankar
mesh_terms: D001487:Basidiomycota; D001947:Breeding; D002874:Chromosome Mapping; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000517:prevention & control; D057865:Plant Immunity / Q000235:genetics*; D018515:Plant Leaves / Q000276:immunology / Q000382:microbiology*; D036226:Seedlings / Q000276:immunology / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D005819:Genetic Markers