title: Evaluation of immunocompatibility of tissue-engineered periosteum.
abstract: Tissue-engineered periosteum (TEP) and 'intramembranous ossification' may be an alternative approach to bone tissue engineering. In the previous study we attained successful bone defect reparation with homemade TEP in an allogenic rabbit model. But its allogenic immunocompatibility remained unknown. In this study TEP was constructed by seeding osteogenically induced mesenchymal stem cells of rabbit onto porcine small intestinal submucosa (SIS). A mixed lymphocyte reaction (MLR) was applied to evaluate the in vitro immunogenicity. The ratio of CD4(+)/CD8(+) T-lymphocytes was tested kinetically to evaluate the systematic reaction of the TEP allograft, and a histological examination was performed to investigate local inflammation and ectopic osteogenesis. MLR indicated that TEP had a higher in vitro immunostimulation than SIS (p < 0.05). The ratios of CD4(+)/CD8(+) lymphocytes increased in both TEP and SIS implanted groups in 2 weeks, followed by a decrease to a normal level from 2 to 4 weeks. Histological examination revealed modest lymphocyte infiltration for no more than 2 weeks. Moreover, subcutaneous ectopic ossification was observed in TEP allograft animals (8/12). Our findings imply that TEP has a certain immune reaction for the allograft, but it is not severe enough to impact osteogenesis in the allogenic rabbit model.
authors.affiliation: Orthopaedic Institute of the 2nd Hospital of Lanzhou University, ChengGuan District, Lanzhou City, People's Republic of China. bonezl@qq.com
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Junli
authors.initials: J
authors.lastname: Zhao
authors.forename: Shuanke
authors.initials: S
authors.lastname: Wang
authors.forename: Yayi
authors.initials: Y
authors.lastname: Xia
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D018786:Bone Substitutes; D016516:CD4-CD8 Ratio; D007413:Intestinal Mucosa / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D010521:Periosteum / Q000276:immunology* / Q000637:transplantation* / Q000648:ultrastructure; D011817:Rabbits; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous
chemical_list: D018786:Bone Substitutes