title: Pharmacokinetics of propranolol hydrochlorid in human urine by capillary electrophoresis coupled with electrochemiluminescence.
abstract: A novel method for the determination of propranolol hydrochlorid (PRO) in human urine was developed using capillary electrophoresis coupled with electrochemiluminescence detection (CE-ECL). The parameters that affected the separation and detection were optimized. Under the optimal conditions, the linear range for PRO was from 0.003 to 2 µg/mL (r(2) = 0.9993), and the detection limit was 1.3 ng/mL (S/N = 3). The method was successfully applied to the study of the pharmacokinetics of PRO in human urine. The relative standard deviations of ECL intensity and migration time were 2.6 and 2.1%, respectively (1.0 µg/mL PRO, n = 6). The recovery was between 96.71 and 97.30%. The peak excretion rate in urine was observed during the 0.5-1 h after oral administration of a 10-mg PRO tablet and the urinary excretion ratio of PRO was 13.6% within 12 h. The method was simple, rapid, economical and sensitive, and may improve the detection of PRO as a doping agent in sports.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, P. R. China. dengby16@163.com
authors.forename: Biyang
authors.initials: B
authors.lastname: Deng
authors.forename: Huihui
authors.initials: H
authors.lastname: Yin
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Ning
mesh_terms: D000328:Adult; D004563:Electrochemistry / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D049449:Luminescence; D011433:Propranolol / Q000493:pharmacokinetics* / Q000652:urine*; D012016:Reference Values
chemical_list: D011433:Propranolol