title: c-Maf-dependent growth of Mycobacterium tuberculosis in a CD14(hi) subpopulation of monocyte-derived macrophages.
abstract: Macrophages are a major component of the innate immune response, comprising the first line of defense against various intracellular pathogens, including Mycobacterium tuberculosis. In this report, we studied the factors that regulate growth of M. tuberculosis H37Rv in subpopulations of human monocyte-derived macrophages (MDMs). In healthy donors, M. tuberculosis H37Rv grew 5.6-fold more rapidly in CD14(hi) MDMs compared with that in CD14(lo)CD16(+) MDMs. Compared with CD14(lo)CD16(+) cells, M. tuberculosis H37Rv-stimulated CD14(hi) monocytes produced more IL-10 and had increased mRNA expression for c-Maf, a transcription factor that upregulates IL-10 gene expression. c-Maf small interfering RNA (siRNA) inhibited IL-10 production and growth of M. tuberculosis in CD14(hi) cells. Compared with CD14(lo)CD16(+) monocytes, M. tuberculosis H37Rv-stimulated CD14(hi) cells had increased expression of 22 genes whose promoters contained a c-Maf binding site, including hyaluronan synthase 1 (HAS1). c-Maf siRNA inhibited HAS1 expression in M. tuberculosis-stimulated CD14(hi) monocytes, and HAS1 siRNA inhibited growth of M. tuberculosis in CD14(hi) MDMs. M. tuberculosis H37Rv upregulated expression of HAS1 protein and its product, hyaluronan, in CD14(hi) MDMs. We conclude that M. tuberculosis grows more rapidly in CD14(hi) than in CD14(lo)CD16(+) MDMs because CD14(hi) cells have increased expression of c-Maf, which increases production of two key factors (hyaluronan and IL-10) that promote growth of M. tuberculosis.
authors.affiliation: Center for Pulmonary and Infectious Disease Control, University of Texas Health Science Center, Tyler, TX 75708, USA.
authors.forename: Rohan
authors.initials: R
authors.lastname: Dhiman
authors.forename: Anuradha
authors.initials: A
authors.lastname: Bandaru
authors.forename: Peter F
authors.initials: PF
authors.lastname: Barnes
authors.forename: Sudipto
authors.initials: S
authors.lastname: Saha
authors.forename: Amy
authors.initials: A
authors.lastname: Tvinnereim
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Nayak
authors.forename: Padmaja
authors.initials: P
authors.lastname: Paidipally
authors.forename: Vijaya Lakshmi
authors.initials: VL
authors.lastname: Valluri
authors.forename: L Vijaya Mohan
authors.initials: LV
authors.lastname: Rao
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Vankayalapati
mesh_terms: D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D014453:Glucuronosyltransferase / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000502:physiology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000502:physiology; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis*; D008264:Macrophages / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000254:growth & development* / Q000276:immunology* / Q000472:pathogenicity; D051257:Proto-Oncogene Proteins c-maf / Q000096:biosynthesis / Q000502:physiology*; D014774:Virulence / Q000276:immunology
chemical_list: D018950:Lipopolysaccharide Receptors; D051257:Proto-Oncogene Proteins c-maf; D016753:Interleukin-10; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases