title: A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries.
abstract: Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
authors.affiliation: Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA.
authors.forename: Sheila
authors.initials: S
authors.lastname: Fisher
authors.forename: Andrew
authors.initials: A
authors.lastname: Barry
authors.forename: Justin
authors.initials: J
authors.lastname: Abreu
authors.forename: Brian
authors.initials: B
authors.lastname: Minie
authors.forename: Jillian
authors.initials: J
authors.lastname: Nolan
authors.forename: Toni M
authors.initials: TM
authors.lastname: Delorey
authors.forename: Geneva
authors.initials: G
authors.lastname: Young
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Fennell
authors.forename: Alexander
authors.initials: A
authors.lastname: Allen
authors.forename: Lauren
authors.initials: L
authors.lastname: Ambrogio
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Berlin
authors.forename: Brendan
authors.initials: B
authors.lastname: Blumenstiel
authors.forename: Kristian
authors.initials: K
authors.lastname: Cibulskis
authors.forename: Dennis
authors.initials: D
authors.lastname: Friedrich
authors.forename: Ryan
authors.initials: R
authors.lastname: Johnson
authors.forename: Frank
authors.initials: F
authors.lastname: Juhn
authors.forename: Brian
authors.initials: B
authors.lastname: Reilly
authors.forename: Ramy
authors.initials: R
authors.lastname: Shammas
authors.forename: John
authors.initials: J
authors.lastname: Stalker
authors.forename: Sean M
authors.initials: SM
authors.lastname: Sykes
authors.forename: Jon
authors.initials: J
authors.lastname: Thompson
authors.forename: John
authors.initials: J
authors.lastname: Walsh
authors.forename: Andrew
authors.initials: A
authors.lastname: Zimmer
authors.forename: Zac
authors.initials: Z
authors.lastname: Zwirko
authors.forename: Stacey
authors.initials: S
authors.lastname: Gabriel
authors.forename: Robert
authors.initials: R
authors.lastname: Nicol
authors.forename: Chad
authors.initials: C
authors.lastname: Nusbaum
mesh_terms: D057205:Automation, Laboratory; D059472:Exome; D015723:Gene Library; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011786:Quality Control