title: Evaluation of superoxide anion radicals generated from an aqueous extract of particulate phase cigarette smoke by electron spin resonance using 5,5-dimethyl-1-pyrroline-N-oxide.
abstract: The reactive oxygen species generated by an aqueous extract of the particulate phase of cigarette smoke were evaluated by an electron spin resonance (ESR) analysis, using spin-trapping agents, and by comparing with model reaction systems. The ESR signals of DMPO-OH were detected from the extract by using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). These signals were eliminated by adding superoxide dismutase, but hardly by catalase. These responses of the ESR signals to the scavengers were similar to those of a hypoxanthine-xanthine oxidase system. The results indicate that the signals of DMPO-OH from the extract were derived from a reaction product of DMPO with superoxide anion radicals and clarify the mechanism by which the extract generated superoxide anion radicals.
authors.affiliation: Japan Tobacco Inc., 6-2 Umegaoka, Aoba-ku, Yokohama, Kanagawa 227-8512, Japan. yuichiro.takanami@jt.com
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Takanami
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D002396:Catechols / Q000737:chemistry; D003497:Cyclic N-Oxides / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D052638:Particulate Matter / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry; D012906:Smoke; D013481:Superoxides / Q000138:chemical synthesis* / Q000737:chemistry*; D014026:Nicotiana; D014867:Water / Q000737:chemistry*
chemical_list: C510912:(diethoxyphosphoryl)-5-methylpyrroline N-oxide; D002396:Catechols; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D006873:Hydroquinones; D052638:Particulate Matter; D011758:Pyrroles; D012906:Smoke; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D006861:Hydrogen Peroxide; C034221:catechol; D010100:Oxygen; C031927:hydroquinone