title: Irreversible changes in protein conformation due to interaction with superparamagnetic iron oxide nanoparticles.
abstract: The understanding of the interactions between nanomaterials and proteins is of extreme importance in medicine. In a biological fluid, proteins can adsorb and associate with nanoparticles, which can have significant impact on the biological behavior of the proteins and the nanoparticles. We report here on the interactions of iron saturated human transferrin protein with both bare and polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles (SPIONs). The exposure of human transferrin to SPIONs results in the release of iron, which changes the main function of the protein, which is the transport of iron among cells. After removal of the magnetic nanoparticles, the original protein conformation is not recovered, indicating irreversible changes in transferrin conformation: from a compact to an open structure.
authors.affiliation: National Cell Bank, Pasteur Institute of Iran, #69 Pasteur Ave., Tehran, 13164, Iran. Mahmoudi@biospion.com
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Soroush
authors.initials: S
authors.lastname: Sardari
authors.forename: Mojgan K
authors.initials: MK
authors.lastname: Moghadam
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Vali
authors.forename: Sophie
authors.initials: S
authors.lastname: Laurent
authors.forename: Pieter
authors.initials: P
authors.lastname: Stroeve
mesh_terms: D001665:Binding Sites; D003911:Dextrans / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D011506:Proteins; C097921:ferumoxtran-10