title: [Suppression of survivin gene in leukemia cells by small interfering RNA].
abstract: OBJECTIVE
  To evaluate the impact of specific siRNA on survivin gene in transfected leukemia cells.
  
  
  METHODS
  The small interfering RNA (siRNA) targeted survivin mRNA was synthesized in vitro and was transfected into K562 cell by Hiperfect into human leukemia cell line K562, which has high survivin expression level. The level of survivin mRNA expression was determined by quantitative reverse transcription polymerase chain reaction (RT-PCR) with SYBR GREEN I. The apoptosis index of cytotrophoblasts were determined and analyzed by FCM (Annexin V-FITC/PI staining methods). The cell proliferation was examined by MTT at 48 h and 72 h after transfection.
  
  
  RESULTS
  The level of mRNA expression was significantly inhibited by the siRNA 48 h and 72 h after transfection, the suppression rate of survivin mRNA separately reached 85.21%, 94.35% mensurated by quantitative RT-PCR with SYBR GREEN I, cell proliferation was inhibited significantly by 45.02% and 50.88%, respectively, the apoptotic rate detected by Annexin V-FITC assay reached 12.28%and 21.55%, respectively.
  
  
  CONCLUSIONS
  The chemosynthesized siRNA targeting survivin could significantly down-regulate survivin mRNA. Survivin siRNA was able to inhibit the proliferation of leukemia cell line K562. Survivin may become a new target for leukemia gene therapy.
authors.affiliation: Department of Pediatric Hematology, Capital Institute of Pediatrics, Beijing 100020, China.
authors.forename: Tian-you
authors.initials: TY
authors.lastname: Wang
authors.forename: Shun-qiao
authors.initials: SQ
authors.lastname: Feng
authors.forename: Zhao-xia
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Shi
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Zi-qin
authors.initials: ZQ
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D020868:Gene Silencing; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D020014:K562 Cells; D034741:RNA, Small Interfering / Q000494:pharmacology*; D000077022:Survivin; D014162:Transfection
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin