title: Efficacy of vaccination with different combinations of MF59-adjuvanted and nonadjuvanted seasonal and pandemic influenza vaccines against pandemic H1N1 (2009) influenza virus infection in ferrets.
abstract: Serum antibodies induced by seasonal influenza or seasonal influenza vaccination exhibit limited or no cross-reactivity against the 2009 pandemic swine-origin influenza virus of the H1N1 subtype (pH1N1). Ferrets immunized once or twice with MF59-adjuvanted seasonal influenza vaccine exhibited significantly reduced lung virus titers but no substantial clinical protection against pH1N1-associated disease. However, priming with MF59-adjuvanted seasonal influenza vaccine significantly increased the efficacy of a pandemic MF59-adjuvanted influenza vaccine against pH1N1 challenge. Elucidating the mechanism involved in this priming principle will contribute to our understanding of vaccine- and infection-induced correlates of protection. Furthermore, a practical consequence of these findings is that during an emerging pandemic, the implementation of a priming strategy with an available adjuvanted seasonal vaccine to precede the eventual pandemic vaccination campaign may be useful and life-saving.
authors.affiliation: Department of Virology, Erasmus Medical Center, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.
authors.forename: Judith M A
authors.initials: JM
authors.lastname: van den Brand
authors.forename: Joost H C M
authors.initials: JH
authors.lastname: Kreijtz
authors.forename: Rogier
authors.initials: R
authors.lastname: Bodewes
authors.forename: Koert J
authors.initials: KJ
authors.lastname: Stittelaar
authors.forename: Geert
authors.initials: G
authors.lastname: van Amerongen
authors.forename: Thijs
authors.initials: T
authors.lastname: Kuiken
authors.forename: James
authors.initials: J
authors.lastname: Simon
authors.forename: Ron A M
authors.initials: RA
authors.lastname: Fouchier
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Del Giudice
authors.forename: Rino
authors.initials: R
authors.lastname: Rappuoli
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D001831:Body Temperature; D001835:Body Weight; D004195:Disease Models, Animal; D005260:Female; D005289:Ferrets; D006651:Histocytochemistry; D007117:Immunization, Secondary / Q000379:methods; D007150:Immunohistochemistry; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008168:Lung / Q000473:pathology; D008297:Male; D008853:Microscopy; D009666:Nose / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000517:prevention & control; D010614:Pharynx / Q000821:virology; D011136:Polysorbates / Q000008:administration & dosage*; D013185:Squalene / Q000008:administration & dosage*; D014611:Vaccination / Q000379:methods; D019562:Viral Load
chemical_list: D000276:Adjuvants, Immunologic; D007252:Influenza Vaccines; C089950:MF59 oil emulsion; D011136:Polysorbates; D013185:Squalene