title: Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain.
abstract: Low-density lipoprotein receptor (LDLR) relative with 11 binding repeats (LR11; also known as sorLA) is genetically associated with late-onset Alzheimer's disease and is thought to be involved in neurodegenerative processes. LR11 contains a vacuolar protein-sorting 10 protein (Vps10p) domain. As this domain has been implicated in protein-protein interaction in other receptors, its structure and function are of great biological interest. Human LR11 Vps10p domain was expressed in mammalian cells and the purified protein was crystallized using the hanging-drop vapour-diffusion method. Enzymatic deglycosylation of the sample was critical to obtaining diffraction-quality crystals. Deglycosylated LR11 Vps10p-domain crystals belonged to the hexagonal space group P6(1)22. A diffraction data set was collected to 2.4 Å resolution and a clear molecular-replacement solution was obtained.
authors.affiliation: Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Zenzaburo
authors.initials: Z
authors.lastname: Nakata
authors.forename: Masamichi
authors.initials: M
authors.lastname: Nagae
authors.forename: Norihisa
authors.initials: N
authors.lastname: Yasui
authors.forename: Hideaki
authors.initials: H
authors.lastname: Bujo
authors.forename: Terukazu
authors.initials: T
authors.lastname: Nogi
authors.forename: Junichi
authors.initials: J
authors.lastname: Takagi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000737:chemistry* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
chemical_list: D026502:LDL-Receptor Related Proteins; D026901:Membrane Transport Proteins; C102582:SORL1 protein, human