title: Crystallization and preliminary X-ray analysis of isopentenyl diphosphate isomerase from Methanocaldococcus jannaschii.
abstract: Type 2 isopentenyl diphosphate isomerase (IDI-2) is a flavoprotein. Recently, flavin has been proposed to play a role as a general acid-base catalyst with no redox role during the enzyme reaction. To clarify the detailed enzyme reaction mechanism of IDI-2 and the unusual role of flavin, structural analysis of IDI-2 from Methanocaldococcus jannaschii (MjIDI) was performed. Recombinant MjIDI was crystallized at 293 K using calcium acetate as a precipitant. The diffraction of the crystal extended to 2.08 Å resolution at 100 K. The crystal belonged to the tetragonal space group I422, with unit-cell parameters a=126.46, c=120.03 Å. The presence of one monomer per asymmetric unit gives a crystal volume per protein weight (VM) of 3.0 Å3 Da(-1) and a solvent constant of 59.0% by volume.
authors.affiliation: Japan Synchrotron Radiation Research Institute (SPring-8), 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hoshino
authors.forename: Eriko
authors.initials: E
authors.lastname: Nango
authors.forename: Seiki
authors.initials: S
authors.lastname: Baba
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D019748:Carbon-Carbon Double Bond Isomerases / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D045782:Hemiterpenes; D017016:Methanococcaceae / Q000201:enzymology*; D008969:Molecular Sequence Data
chemical_list: D001426:Bacterial Proteins; D045782:Hemiterpenes; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase