title: The Drosophila Myc gene, diminutive, is a positive regulator of the Sex-lethal establishment promoter, Sxl-Pe.
abstract: The binary switch gene Sex-lethal (Sxl) controls sexual identity in Drosophila. When activated, Sxl imposes female identity, whereas male identity ensues by default when the gene is off. The decision to activate Sxl is controlled by an X chromosome counting system that regulates the Sxl establishment promoter, Sxl-Pe. The counting system depends upon the twofold difference in the gene dose of a series of X-linked transcription factors or numerators. Because of this difference in dose, early female embryos express twice the amount of these transcription factors, and the cumulative action of these transcription factors turns on Sxl-Pe. Here we show that the Drosophila Myc gene diminutive is an X-linked numerator.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Gretchen
authors.initials: G
authors.lastname: Kappes
authors.forename: Girish
authors.initials: G
authors.lastname: Deshpande
authors.forename: Brett B
authors.initials: BB
authors.lastname: Mulvey
authors.forename: Jamila I
authors.initials: JI
authors.lastname: Horabin
authors.forename: Paul
authors.initials: P
authors.lastname: Schedl
mesh_terms: D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005804:Genes, Lethal / Q000235:genetics; D050172:Genes, X-Linked / Q000235:genetics; D008297:Male; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C102284:Myc protein, Drosophila; C067777:Sxl protein, Drosophila; C078020:Dpn protein, Drosophila