title: Antimicrobial activity of some new of 2-(4-ethyl-phenoximethyl) benzoic acid thioureides against planktonic cells.
abstract: The aim of the present study was to evaluate the antimicrobial activity of new 2-(4-ethyl-phenoxymethyl) benzoic acid thioureides. The synthesis of the new compounds was done in three steps starting with the synthesis of 2-(4-ethyl-phenoxymethyl) benzoic acid. In the second stage, the 2-(4-ethyl-phenoxymethyl)-benzoyl chloride was prepared and the new thioureides were synthesized in the third step by the reaction of 2-(4-ethyl-phenoxymethyl) benzoyl isothiocyanate with various primary aromatic amines. The original compounds were screened for their in vitro antimicrobial activity against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) as well as to fungal strains (Candida albicans, Aspergillus niger), using both reference and clinical, multidrug resistant strains. The qualitative screening of the susceptibility spectra of various microbial strains versus these compounds was performed by three adaptated diffusion methods: (1) impregnation of the filter paper disks with the respective substance solutions, (2) distribution of the tested solutions into agar wells and (3) spotting of the respective solutions on the solid medium previously inoculated with the microbial suspensions. The quantitative assay of the antimicrobial activity was performed by broth microdilution method in 96-well microplates in order to establish the minimal inhibitory concentration (MIC). The tested compounds exhibited specific antimicrobial activity with MICs ranging from 3.9 microg/mL to 250 microg/mL.
authors.affiliation: National Institute of Research-Development for Microbiology and Immunology Cantacuzino, Spl. Independentei, 103, 050096 Bucharest, Romania. microcult@cantacuzino.ro
authors.forename: Olguţa
authors.initials: O
authors.lastname: Drăcea
authors.forename: Camelia
authors.initials: C
authors.lastname: Babeş
authors.forename: Carmen
authors.initials: C
authors.lastname: Limban
authors.forename: Cristina
authors.initials: C
authors.lastname: Delcaru
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.forename: Anca-Michaela
authors.initials: AM
authors.lastname: Israil
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010933:Plankton / Q000166:cytology / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D001565:Benzoates