title: Predominance of IgG1 and IgG3 subclasses of autoantibodies to peptidylarginine deiminase 4 in rheumatoid arthritis.
abstract: Analysis of IgG subclass distribution of antibodies may provide insights into the mechanisms driving antibody production. Here, we have first determined IgG subclass distribution of anti-recombinant peptidylarginine deiminase 4 (anti-hPADI4) antibodies in sera from patients with rheumatoid arthritis (RA). Sera from 103 RA patients were screened to IgG anti-PADI4 using recombinant antigens. For positive samples, the subclass distribution of IgG anti-hPADI4 was determined by means of establishing the equipotency of four HRP-labeled IgG subclass-specific monoclonal antibodies. The subclass profiles were compared among individuals with different disease status. Thirty-three out of 103 RA patients were determined as IgG anti-hPADI4-positive. As expressed by percentage levels to total IgG anti-hPADI4 activity, IgG1 and IgG3 were determined to be the predominant subclasses of anti-hPADI4. Furthermore, distinct subclass distribution patterns were observed in patients with different disease status. The IgG subclass distribution of anti-hPADI4 indicates that a leading T lymphocyte-regulated IgG1 and IgG3 responses may contribute to a better understanding of the role of anti-PADI4 in RA.
authors.affiliation: Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jinming
authors.initials: J
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology*; D015331:Cohort Studies; D005260:Female; D006801:Humans; D006867:Hydrolases / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D000076342:Protein-Arginine Deiminases; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D006867:Hydrolases; D000076342:Protein-Arginine Deiminases