title: Measurement of total antioxidant activity with chlorpromazine radical cation.
abstract: In the present study there is described a simple and sensitive method for the evaluation of antioxidant activity in chlorpromazine-Fe (III) system using ascorbic acid as standard.
  
  
  METHODS
  Chlorpromazine [2-chloro-N-(3-dimethyl-aminopropyl)-phenothiazine] is oxidized in acidic media by Fe (III) with the formation of a stable radical cation characterized by an intense visible absorbtion band (lambda = 540 nm). The optimum parameters for the stability of the radical cation have been studied: molar ratio chlorpromazine-Fe (III), pH, solvents. Spectrophotometric methods have been used in these studies.
  
  
  RESULTS
  The chlorpromazine radical cation is stable in the acidic media (pH = 3) at a molar ratio chlorpromazine: Fe (III) of 2: 1. Ascorbic acid reduces these radicals and induces a decrease of absorbance. Percent inhibition was calculated and plotted as a function of the concentration of standard antioxidant solutions. The results show that percent inhibition varies in a linear manner with the ascorbic acid concentration. Percent inhibition is higher when the antioxidant solution is added after generation of radical cation.
  
  
  CONCLUSIONS
  It has been developed a method for evaluating antioxidant activity in the chlorpromazine: Fe (III) system using ascorbic acid as a standard. The method is fast, simple and sensitive; it can be applied for the detection and evaluation of the antioxidant activity of simple or complex systems.
authors.affiliation: Department of General and Inorganic Chemistry, School of Pharmacy, Gr.T. Popa University of Medicine and Pharmacy Iaşi.
authors.forename: Alina Monica
authors.initials: AM
authors.lastname: Miftode
authors.forename: Alina
authors.initials: A
authors.lastname: Stefanache
authors.forename: Maria
authors.initials: M
authors.lastname: Stan
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D014150:Antipsychotic Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000737:chemistry; D002412:Cations; D002746:Chlorpromazine / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron; D012997:Solvents; D013053:Spectrophotometry
chemical_list: D000975:Antioxidants; D014150:Antipsychotic Agents; D002412:Cations; D005609:Free Radicals; D012997:Solvents; D007501:Iron; D001205:Ascorbic Acid; D002746:Chlorpromazine