title: Proteome profiling of heat shock of human primary breast epithelial cells, a dataset report.
abstract: Exposure to elevated temperatures has a strong effect on cell functions, and is used in clinical practice. Hyperthermia may affect multiple regulatory mechanisms in cells. To understand better the response to hyperthermia of immortalized primary human breast epithelial cells, we performed a proteomics study of these cells cultured at 34°C or 39°C. Twenty-four proteins were shown to be differentially expressed due to hyperthermia. Analysis of these proteins showed the potential involvement of various biological processes in response to hyperthermia, e.g., cell adhesion, cell communication, and cell cycle. Transforming growth factor-β2 (TGF-β2) and heat shock protein 27 (HSP27) were found to be upregulated at 39°C. TGF-β2 was found to affect expression of HSP27, and to have a protective role in hyperthermia-induced cell death. Thus, the dataset described here of hyperthermia-related proteins in human primary breast epithelial cells predicts a number of cellular activities affected by exposure to high temperatures and provides a set of proteins for further studies.
authors.affiliation: Department of Oncology-Pathology, Karolinska Biomics Center, Karolinska Institutet, Stockholm, Sweden. min.jia@ki.se
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.forename: Serhiy
authors.initials: S
authors.lastname: Souchelnytskyi
mesh_terms: D001940:Breast / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response; D006358:Hot Temperature; D006801:Humans; D020543:Proteome; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D055551:HSP27 Heat-Shock Proteins; D020543:Proteome; D016212:Transforming Growth Factor beta