title: Efficient propagation of progressive multifocal leukoencephalopathy-type JC virus in COS-7-derived cell lines stably expressing Tat protein of human immunodeficiency virus type 1.
abstract: The high incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients compared with many other immunosuppressive diseases suggests that HIV-1 infection is strictly related to the activation of JC virus (JCV) propagation. In this report, propagation of PML-type JCV in COS-7-derived cell lines stably expressing HIV-1 Tat (COS-tat cells) has been examined. In COS-tat cells, production of viral particles and replication of genomic DNA were markedly increased compared to COS-7 cells, as judged by HA and real-time PCR analyses. These results demonstrate that COS-tat cells provide a useful model system for studying HIV-1 Tat-mediated propagation of PML-type JCV.
authors.affiliation: Department of Microbiology, Kobe Institute of Health, Minatojima-Nakamachi, Kobe, Hyogo, Japan. s-nuku@gj8.so-net.ne.jp
authors.forename: Souichi
authors.initials: S
authors.lastname: Nukuzuma
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakamichi
authors.forename: Masanori
authors.initials: M
authors.lastname: Kameoka
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sugiura
authors.forename: Chiyoko
authors.initials: C
authors.lastname: Nukuzuma
authors.forename: Isao
authors.initials: I
authors.lastname: Miyoshi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Takegami
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D015497:HIV-1 / Q000502:physiology*; D007577:JC Virus / Q000254:growth & development*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000502:physiology*
chemical_list: D054322:tat Gene Products, Human Immunodeficiency Virus