title: Simultaneous determination and pharmacokinetic study of metformin and rosiglitazone in human plasma by HPLC-ESI-MS.
abstract: A fast, sensitive and specific high-performance liquid chromatography tandem mass spectrometry method was developed for simultaneous determination of metformin and rosiglitazone in human plasma. With phenformin as an internal standard, the analysis was carried out on a C(18) column (50 mm × 2.1 mm, 3.5 μm) using a mobile phase consisting of acetonitrile-10 mM ammonium acetate (20:80, v/v). The detection was performed by tandem mass spectrometry via electrospray ionization. Linear calibration curves were obtained in the concentration of 1.054-263.5 ng/mL for rosiglitazone and 4.040-5050 ng/mL for metformin. The method was applicable to clinical pharmacokinetic study of metformin and rosiglitazone in healthy volunteers following oral administration.
authors.affiliation: Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, RP China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Mei
authors.initials: M
authors.lastname: Shen
authors.forename: Ande
authors.initials: A
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D008687:Metformin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D010629:Phenformin / Q000032:analysis; D015203:Reproducibility of Results; D000077154:Rosiglitazone; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D045162:Thiazolidinediones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D045162:Thiazolidinediones; D000077154:Rosiglitazone; D008687:Metformin; D010629:Phenformin