title: [Genome shuffling method of Bacillus subtilis].
abstract: Genome shuffling methods were explored for Bacillus subtilis strain molecular breeding. Recycling protoplast fusion, recycling transformation and recycling universal transduction were used for genome shuffling in B. subtilis. Four strains with different nutrition-deficiency markers were used as initial strains. After five rounds protoplast fusion, transformation or transduction, the descendant with 4 markers had not been detected, and the rate of descendant with 3 markers were 4.53 x 10(-4), 1.64 x 10(-4), 4.47 x 10(-3), respectively. A computer program was made to simulate the recycling fusion process. Based on simulation result and comparing the genome shuffling result of B. subtilis in this experiment and that of Streptomyces coelicolor reported in references, effective genome shuffling needs a high recombination rate of at least between 10(-3) and 10(-2).
authors.affiliation: Key Laboratory of Synthetic Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yang
authors.forename: Wenchao
authors.initials: W
authors.lastname: Fan
authors.forename: Han
authors.initials: H
authors.lastname: Xiao
authors.forename: Chunhong
authors.initials: C
authors.lastname: Guan
authors.forename: Chuanzeng
authors.initials: C
authors.lastname: Cao
authors.forename: Haifeng
authors.initials: H
authors.lastname: Shao
authors.forename: Weihong
authors.initials: W
authors.lastname: Jiang
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
mesh_terms: D001412:Bacillus subtilis / Q000145:classification / Q000235:genetics*; D032621:DNA Shuffling; D005821:Genetic Techniques; D016680:Genome, Bacterial / Q000235:genetics*; D015202:Protein Engineering; D014169:Transformation, Bacterial