title: Starch granule-associated proteins of hull-less barley (Hordeum vulgare L.) from the Qinghai-Tibet Plateau in China.
abstract: BACKGROUND
  The starch granule-associated proteins (SGAPs) are the minor components of the starch granules and a majority of them are believed to be starch biosynthetic enzymes. The Qinghai-Tibet Plateau in China, one of the centres of origin of cultivated barley, is abundant in hull-less barley resources which exhibit high polymorphism in SGAPs.
  
  
  RESULTS
  The SGAPs of hull-less barley from Qinghai-Tibet Plateau were analysed by one-dimensional (1-D) SDS-PAGE, 2-D PAGE and ESI-Q-TOF MS/MS. In the 1-D SDS-PAGE gel, four proteins including a 80 kDa starch synthase, actin, actin 4 and ATP synthase β-subunit were identified as novel SGAPs. A total of six different bands were identified as starch granule-bound starch synthase I (GBSSI) and the segregation of the novel GBSSI bands in F(1) and F(2) seeds derived from yf127 × yf70 was in accordance with Mendel's law. In the 2-D PAGE gel, 92 spots were identified as 42 protein species which could be classified into 15 functional groups. Thirteen protein species were identified as SGAPs for the first time and multiple spots were identified as GBSSI.
  
  
  CONCLUSIONS
  This study revealed novel SGAPs in hull-less barley from the Qinghai-Tibet Plateau in China and these will be significant in further studies of starch biosynthesis in barley.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, No. 9 Section 4, Renmin South Road, Chengdu 610041, People's Republic of China.
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Wang
authors.forename: Zhi-Fen
authors.initials: ZF
authors.lastname: Pan
authors.forename: Zha-Xi
authors.initials: ZX
authors.lastname: Nima
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Tang
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Liang
authors.forename: Guang-Bing
authors.initials: GB
authors.lastname: Deng
authors.forename: Hai
authors.initials: H
authors.lastname: Long
authors.forename: Mao-Qun
authors.initials: MQ
authors.lastname: Yu
mesh_terms: D025181:ATP Synthetase Complexes / Q000032:analysis / Q000235:genetics; D000199:Actins / Q000032:analysis / Q000235:genetics; D000595:Amino Acid Sequence; D002681:China; D004591:Electrophoresis, Polyacrylamide Gel; D001467:Hordeum / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D011110:Polymorphism, Genetic; D012639:Seeds / Q000737:chemistry*; D013213:Starch / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013214:Starch Synthase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D018609:Tibet
chemical_list: D000199:Actins; D010940:Plant Proteins; D013213:Starch; D013214:Starch Synthase; D025181:ATP Synthetase Complexes