title: [Analysis of chromosomal abnormalities in pancreatic cancer by spectral karyotyping].
abstract: OBJECTIVE
  to investigate the chromosomal characteristics of pancreatic ductal adenocarcinomas by spectral karyotyping.
  
  
  METHODS
  cytogenetic aberrations of pancreatic cancer cell line P2 established from a Chinese patient was investigated by spectral karyotyping (SKY). Chromosomal alterations were further evaluated in 10 cases of pancreatic cancer and 10 cases of chronic pancreatitis by two color fluorescence in situ hybridization (FISH) by using EGFR/CEP7 probe and paraffin embedded tissue samples.
  
  
  RESULTS
  hypotriploid and 26 chromosomal aberrations were revealed in cell line P2. Recurrent chromosomal numerical alterations included loss of chromosome 4, 9, 18, 19, 22, Y, 10p, 15p, 8p, 6q and 12p, with gain of chromosome 7 and 12q. Frequent chromosomal structural abnormalities included der(9;15)(q10;q10), der(10)(3;10)(?;q26) and der(12)(8;12)(?;p13). Four of 10 cases showed EGFR copy number changes by FISH.
  
  
  CONCLUSIONS
  highly complex chromosomal rearrangements occur in pancreatic cancers. Additional studies of more cases are needed, including FISH analysis of EGFR copy number changes, to reach a conclusion.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China.
authors.forename: Zhi-Mei
authors.initials: ZM
authors.lastname: Yang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Han
authors.forename: Sha-Fei
authors.initials: SF
authors.lastname: Wu
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Yin
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liang
authors.forename: Xuan
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000368:Aged; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D058674:Chromosome Duplication; D005260:Female; D018628:Gene Dosage; D018773:Genes, erbB-1 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics*