title: Pentose phosphate pathway flux analysis for glycopeptide antibiotic vancomycin production during glucose-limited cultivation of Amycolatopsis orientalis.
abstract: In vivo pentose phosphate pathway (PPP) enzymes such as glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and transaldolase (TAL) activities as well as ATP- and ADP-level variations of Amycolatopsis orientalis were investigated with respect to glucose concentration and incubation period. G6PDH, 6PGDH, and TAL activities of A. orientalis reached maximum levels at 48 hr for all glucose concentrations used, after which the levels began to decline. G6PDH, 6PGDH, and TAL activities showed positive correlation with the glucose concentration up to 15 g/L, while further increases had an opposite effect. Intracellular ATP level showed a positive correlation with glucose concentrations, while ADP level increased up to 15 g/L. ATP concentration of A. orientalis increased rapidly at 48 hr of incubation, as was the case also for G6PDH, 6PGDH, and TAL activities, although the incubation period corresponding to maximum values of ADP shifted to 60 hr. Production of the glycopeptide antibiotic vancomycin increased with the increases in glucose concentrations up to 15 g/L, by showing coherence in the rates of oxidative and nonoxidative parts of the PPP.
authors.affiliation: Faculty of Science, Department of Chemistry, Biochemistry Division, University of Dokuz Eylul, Buca, Izmir, Turkey. hulya.kayali@deu.edu.tr
authors.forename: Hulya
authors.initials: H
authors.lastname: Ayar-Kayali
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000254:growth & development; D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000502:physiology*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D014153:Transaldolase / Q000032:analysis / Q000378:metabolism*; D014640:Vancomycin / Q000032:analysis / Q000096:biosynthesis
chemical_list: D000900:Anti-Bacterial Agents; D005942:Gluconates; D019298:Glucose-6-Phosphate; D000244:Adenosine Diphosphate; D014640:Vancomycin; D000255:Adenosine Triphosphate; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D014153:Transaldolase; D005947:Glucose; C008884:6-phosphogluconic acid