title: Galleria mellonella as an infection model for Campylobacter jejuni virulence.
abstract: Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.
authors.affiliation: School of BioSciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, UK.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Senior
authors.affiliation: Veterinary Laboratories Agency, Woodham Lane, Addlestone, UK.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Bagnall
authors.affiliation: School of BioSciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, UK.
authors.forename: Olivia L
authors.initials: OL
authors.lastname: Champion
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath, UK.
authors.forename: Stuart E
authors.initials: SE
authors.lastname: Reynolds
authors.affiliation: Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.|Veterinary Laboratories Agency, Woodham Lane, Addlestone, UK.
authors.forename: Roberto M
authors.initials: RM
authors.lastname: La Ragione
authors.affiliation: Veterinary Laboratories Agency, Woodham Lane, Addlestone, UK.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.affiliation: Veterinary Laboratories Agency, Woodham Lane, Addlestone, UK.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Salguero
authors.affiliation: School of BioSciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, UK.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000209:etiology*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D002460:Cell Line; D004195:Disease Models, Animal; D006434:Hemocytes / Q000382:microbiology; D006801:Humans; D007814:Larva / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D009036:Moths / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D018411:Spodoptera / Q000382:microbiology; D014774:Virulence / Q000235:genetics