title: Ellagic acid-induced hypercoagulable state in animals: a potentially useful animal hypercoagulable model for evaluation of anticoagulants.
abstract: OBJECTIVE
  To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants.
  
  
  METHODS
  Forty mice, thirty-two rats, and twenty-four rabbits were randomly and equally divided into control group (saline) and three ellagic acid (EA)-treated groups (low, middle, and high doses). In the mice, bleeding time (BT) was estimated with tail transaction, and clotting time (CT) with template method. Prothrombin time (PT) and the activated partial thromboplastin time (APTT) in rats and rabbits were measured by means of Quick's one-stage assay and modified APTT assay respectively. In addition, thrombin activity was estimated in rats with PT assay using a hemagglutination analyzer. The circulating platelet aggregates were detected in rabbits through platelet counting and presented as the circulating platelet aggregate ratio (CPAR).
  
  
  RESULTS
  EA shortened BT and CT in mice, PT and APTT in rats, and increased thrombin activity and CPAR, all in a dose-dependent manner. EA also brought reduction of PT and APTT in rabbits in dose- and time-dependent manners.
  
  
  CONCLUSIONS
  EA could induce hypercoagulable state through activating coagulation system and platelets in mice, rats, and rabbits.
authors.affiliation: Department of Pharmacology, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Na
authors.forename: Liu
authors.initials: L
authors.lastname: Jun-tian
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qiang-zong
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use*; D001778:Blood Coagulation Disorders / Q000139:chemically induced* / Q000188:drug therapy*; D004353:Drug Evaluation, Preclinical; D004610:Ellagic Acid / Q000009:adverse effects*; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D010974:Platelet Aggregation / Q000187:drug effects; D011517:Prothrombin Time; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000925:Anticoagulants; D004610:Ellagic Acid