title: Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population.
abstract: Nested association mapping (NAM) offers power to resolve complex, quantitative traits to their causal loci. The maize NAM population, consisting of 5,000 recombinant inbred lines (RILs) from 25 families representing the global diversity of maize, was evaluated for resistance to southern leaf blight (SLB) disease. Joint-linkage analysis identified 32 quantitative trait loci (QTLs) with predominantly small, additive effects on SLB resistance. Genome-wide association tests of maize HapMap SNPs were conducted by imputing founder SNP genotypes onto the NAM RILs. SNPs both within and outside of QTL intervals were associated with variation for SLB resistance. Many of these SNPs were within or near sequences homologous to genes previously shown to be involved in plant disease resistance. Limited linkage disequilibrium was observed around some SNPs associated with SLB resistance, indicating that the maize NAM population enables high-resolution mapping of some genome regions.
authors.affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Kump
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Bradbury
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Wisser
authors.forename: Edward S
authors.initials: ES
authors.lastname: Buckler
authors.forename: Araby R
authors.initials: AR
authors.lastname: Belcher
authors.forename: Marco A
authors.initials: MA
authors.lastname: Oropeza-Rosas
authors.forename: John C
authors.initials: JC
authors.lastname: Zwonitzer
authors.forename: Stephen
authors.initials: S
authors.lastname: Kresovich
authors.forename: Michael D
authors.initials: MD
authors.lastname: McMullen
authors.forename: Doreen
authors.initials: D
authors.lastname: Ware
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Balint-Kurti
authors.forename: James B
authors.initials: JB
authors.lastname: Holland
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D004843:Epistasis, Genetic; D017343:Genes, Plant; D055106:Genome-Wide Association Study; D005838:Genotype; D006239:Haplotypes; D007113:Immunity, Innate; D020161:Physical Chromosome Mapping; D010935:Plant Diseases / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D003313:Zea mays / Q000235:genetics*