title: Ultrastructure of isolated mouse ovarian follicles cultured in vitro.
abstract: BACKGROUND
  In vitro maturation of ovarian follicles, in combination with cryopreservation, might be a valuable method for preserving and/or restoring fertility in mammals with impaired reproductive function. Several culture systems capable of sustaining mammalian follicle growth in vitro have been developed and many studies exist on factors influencing the development of in vitro grown oocytes. However, a very few reports concern the ultrastructural morphology of in vitro grown follicles.
  
  
  METHODS
  The present study was designed to evaluate, by transmission and scanning electron microscopy, the ultrastructural features of isolated mouse preantral follicles cultured in vitro for 6 days in a standard medium containing fetal calf serum (FCS). The culture was supplemented or not with FSH.
  
  
  RESULTS
  The follicles cultured in FCS alone, without FSH supplementation (FCS follicles), did not form the antral cavity. They displayed low differentiation (juxta-nuclear aggregates of organelles in the ooplasm, a variable amount of microvilli on the oolemma, numerous granulosa cell-oolemma contacts, signs of degeneration in granulosa cell compartment). Eighty (80)% of FSH-treated follicles formed the antral cavity (FSH antral follicles). These follicles showed various ultrastructural markers of maturity (spreading of organelles in ooplasm, abundant microvilli on the oolemma, scarce granulosa cell-oolemma contacts, granulosa cell proliferation). Areas of detachment of the innermost granulosa cell layer from the oocyte were also found, along with a diffuse granulosa cell loosening compatible with the antral formation. Theca cells showed an immature morphology for the stage reached. Twenty (20)% of FSH-treated follicles did not develop the antral cavity (FSH non-antral follicles) and displayed morphological differentiation features intermediate between those shown by FCS and FSH antral follicles (spreading of organelles in the ooplasm, variable amount of microvilli, scattered granulosa cell-oolemma contacts, signs of degeneration in granulosa cell compartment).
  
  
  CONCLUSIONS
  It is concluded that FSH supports the in vitro growth of follicles, but the presence of a diffuse structural granulosa cell-oocyte uncoupling and the absence of theca development unveil the incomplete efficiency of the system. The present study contributes to explain, from a morphological point of view, the effects of culture conditions on the development of mouse in vitro grown follicles and to highlight the necessity of maintaining efficient intercellular communications to obtain large numbers of fully-grown mature germ cells.
authors.affiliation: Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, La Sapienza University of Rome, Rome, Italy.
authors.forename: Stefania A
authors.initials: SA
authors.lastname: Nottola
authors.forename: Sandra
authors.initials: S
authors.lastname: Cecconi
authors.forename: Serena
authors.initials: S
authors.lastname: Bianchi
authors.forename: Cecilia
authors.initials: C
authors.lastname: Motta
authors.forename: Gianna
authors.initials: G
authors.lastname: Rossi
authors.forename: Maria A
authors.initials: MA
authors.lastname: Continenza
authors.forename: Guido
authors.initials: G
authors.lastname: Macchiarelli
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000648:ultrastructure; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009865:Oocytes / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D013799:Theca Cells / Q000648:ultrastructure; D046509:Tissue Culture Techniques
chemical_list: D005640:Follicle Stimulating Hormone