title: Effects of the dichloromethane fraction of Dipsaci Radix on the osteoblastic differentiation of human alveolar bone marrow-derived mesenchymal stem cells.
abstract: Dipsaci Radix is the dried root of Dipsacus asper Wall. It has been used in Korean herbal medicine to treat bone fractures. In this study, we examined the effect of the dichloromethane fraction of Dipsaci Radix (DR(DM)) on the osteoblastic differentiation of human alveolar bone marrow-derived MSCs (ABM-MSCs). The ABM-MSCs were isolated from healthy subjects and cultured in vitro, followed by phenotypic characterization. They showed a fibroblast-like morphology and expressed CD29, CD44, CD73, and CD105, but not CD34. Calcified nodules were generated in response to both dexamethasone (DEX) and DR(DM). There was a significant increase in the alkaline phosphatase (ALP) activity and protein expression of bone sialoprotein (BSP) and osteocalcin (OC) in response to DEX and DR(DM) as compared to control. These results provide evidence for the osteogenic potential of cultured ABM-MSCs in response to DR(DM). Also, an active single compound was additionally isolated from DR(DM). The single compound (hederagenin 3-O-(2-O-acetyl)-α-L-arabinopyranoside) also significantly increased ALP activity and the level of protein expression of BSP and OC. These results highlight the possible clinical applications of DR(DM) and hederagenin 3-O-(2-O-acetyl)-α-L-arabinopyranoside in bone regeneration.
authors.affiliation: Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, Korea.
authors.forename: Beom-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Yoon-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Homa
authors.initials: H
authors.lastname: Zadeh
authors.forename: Yoon-Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Pi
authors.forename: Hyung-Shik
authors.initials: HS
authors.lastname: Shin
authors.forename: Hyung-Keun
authors.initials: HK
authors.lastname: You
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049917:Dipsacaceae / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008752:Methylene Chloride / Q000032:analysis / Q000494:pharmacology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D010641:Phenotype
chemical_list: D008752:Methylene Chloride; D000469:Alkaline Phosphatase; D002118:Calcium