title: Placental transfer of the insulin analog glargine in the ex vivo perfused placental cotyledon model.
abstract: OBJECTIVE
  The aim was to characterize the transfer of the insulin analog glargine across the placenta using the placental perfusion model.
  
  
  METHODS
  Placentas were obtained and selected cotyledons were cannulated and dually perfused. Glargine, 50 mU/L (n = 2) and 200 mU/L (n = 1), and a reference marker, antipyrine (50 μg/mL), were added to the maternal circulation. Samples were taken from the maternal and fetal compartments.
  
  
  RESULTS
  Glargine was not detected in the fetal compartment. In the maternal compartment, the steady state concentration was 50% lower than the starting concentration.
  
  
  CONCLUSIONS
  Glargine probably does not cross the human placenta. Reduced maternal steady state concentrations may suggest insulin uptake by the placenta.
authors.affiliation: Department of Obstetrics and Gynecology, E. Wolfson Medical Center, Holon, Sackler School of Medicine, Tel Aviv University, Israel. michalkovo@gmail.com [corrected]
authors.forename: Michal
authors.initials: M
authors.lastname: Kovo
authors.forename: Julio
authors.initials: J
authors.lastname: Wainstein
authors.forename: Zipora
authors.initials: Z
authors.lastname: Matas
authors.forename: Simon
authors.initials: S
authors.lastname: Haroutiunian
authors.forename: Amnon
authors.initials: A
authors.lastname: Hoffman
authors.forename: Abraham
authors.initials: A
authors.lastname: Golan
mesh_terms: D000983:Antipyrine / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D007004:Hypoglycemic Agents / Q000378:metabolism*; D066298:In Vitro Techniques; D007328:Insulin / Q000031:analogs & derivatives* / Q000378:metabolism; D000069036:Insulin Glargine; D049528:Insulin, Long-Acting; D008431:Maternal-Fetal Exchange; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D049528:Insulin, Long-Acting; D000069036:Insulin Glargine; D000983:Antipyrine