title: Antimicrobial activity of diterpenes from Viguiera arenaria against endodontic bacteria.
abstract: Six pimarane-type diterpenes isolated from Viguiera arenaria Baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia, Prevotella buccae, Fusobacterium nucleatum, Bacteroides fragilis, Actinomyces naeslundii, Actinomyces viscosus, Peptostreptococcus micros, Enterococcus faecalis and Aggregatibacter actinomycetemcomitans. The compounds ent-pimara-8(14),15-dien-19-oic acid, its sodium salt and ent-8(14),15-pimaradien-3β-ol were the most active, displaying MIC values ranging from 1 to 10 μg mL-1. The results also allow us to conclude that minor structural differences among these diterpenes significantly influence their antimicrobial activity, bringing new perspectives to the discovery of new chemicals for use as a complement to instrumental endodontic procedures.
authors.affiliation: Laboratório de Pesquisa em Microbiologia Aplicada, Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil.
authors.forename: Tatiane C
authors.initials: TC
authors.lastname: Carvalho
authors.forename: Marília R
authors.initials: MR
authors.lastname: Simão
authors.forename: Sérgio R
authors.initials: SR
authors.lastname: Ambrósio
authors.forename: Niege A J C
authors.initials: NA
authors.lastname: Furtado
authors.forename: Rodrigo C S
authors.initials: RC
authors.lastname: Veneziani
authors.forename: Vladimir C G
authors.initials: VC
authors.lastname: Heleno
authors.forename: Fernando B
authors.initials: FB
authors.lastname: Da Costa
authors.forename: Brenda P F A
authors.initials: BP
authors.lastname: Gomes
authors.forename: Maria Gorete M
authors.initials: MG
authors.lastname: Souza
authors.forename: Erika
authors.initials: E
authors.lastname: Borges dos Reis
authors.forename: Carlos H G
authors.initials: CH
authors.lastname: Martins
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes