title: Modulation of the apoptotic pathway in skeletal muscle models: the role of growth hormone.
abstract: Despite numerous studies on the role of growth hormone (GH), its function in skeletal muscle apoptosis secondary to various stimuli is poorly understood. In this study, we used rodent muscle cell lines to analyse cell growth and survival as well as the morphological and molecular markers of cell death in C2C12 and L6C5 myoblasts. These cells were treated either in the presence or absence of GH under serum starvation conditions or in the pro-apoptotic concentrations of hydrogen peroxide (H2O2). Although the cells were responsive to the presence of GH, we did not observe GH modulation of cell growth and survival. The presence of GH did not affect the cell death programme or the expression of apoptotic markers in basal conditions or under oxidative stress. In conclusion, this study indicated that GH "by itself" is not effective in modulating the intracellular pathways leading to cell survival or cell death induced by apoptotic stimuli.
authors.affiliation: Department of Health Science, University of Rome Foro Italico Piazza Lauro De Bosis 15, 00194, Rome, Italy. ivan.dimauro@uniroma4.it
authors.forename: Ivan
authors.initials: I
authors.lastname: Dimauro
authors.forename: Fiorenza
authors.initials: F
authors.lastname: Magi
authors.forename: Gina
authors.initials: G
authors.lastname: La Sala
authors.forename: Monica
authors.initials: M
authors.lastname: Pittaluga
authors.forename: Paolo
authors.initials: P
authors.lastname: Parisi
authors.forename: Daniela
authors.initials: D
authors.lastname: Caporossi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D018384:Oxidative Stress
chemical_list: D019382:Human Growth Hormone