title: Targeting NCA-95 and other granulocyte antigens and receptors with radiolabeled monoclonal antibodies (Mabs).
abstract: During the last decade considerable effort has been made in the research for in vivo techniques of labeling neutrophils with peptides, labeled cytokines and (99m)Tc-labeled antigranulocyte monoclonal antibodies (AG-Mabs). In general the advantage of in vivo labeling is the simplicity of this approach compared with in vivo techniques. Three of these AG-Mabs have been evaluated in clinical studies: Besilesomab (Scintimun®), Sulesomab (Leucoscan®) and Fanolesomab (Leu-Tech®). White blood cells (WBCs) radiolabeled with AG-Mabs do not show the same behaviour as in vivo labeled white blood cells. Especially (99m)Tc-Sulesomab and (99m)Tc-Besilesomab image infectious foci mainly by non-specific extravasation with secondary binding to postmigratory leukocytes already present at the site of infection.
authors.affiliation: Department of Nuclear Medicine, University of Göttingen, Göttingen, Germany. jmeller@med.uni-goettingen.de
authors.forename: J
authors.initials: J
authors.lastname: Meller
authors.forename: T
authors.initials: T
authors.lastname: Liersch
authors.forename: M M
authors.initials: MM
authors.lastname: Oezerden
authors.forename: C O
authors.initials: CO
authors.lastname: Sahlmann
authors.forename: B
authors.initials: B
authors.lastname: Meller
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D015703:Antigens, CD / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D058948:Cell Tracking / Q000379:methods; D016503:Drug Delivery Systems / Q000639:trends; D058851:GPI-Linked Proteins / Q000378:metabolism; D006098:Granulocytes / Q000000981:diagnostic imaging* / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D011868:Radioisotopes / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D011956:Receptors, Cell Surface