title: Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method.
abstract: Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost-effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method.
authors.affiliation: CEA-DSV/iRTSV/LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA-Grenoble, Grenoble, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Aude-Garcia
authors.forename: Véronique
authors.initials: V
authors.lastname: Collin-Faure
authors.forename: Sylvie
authors.initials: S
authors.lastname: Luche
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional / Q000191:economics / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis*; D006801:Humans; D009942:Organometallic Compounds / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D011506:Proteins; D020543:Proteome; C518776:ruthenium II tris (bathophenanthroline disulfonate)