title: Placental structure and inflammation in pregnancies associated with obesity.
abstract: BACKGROUND
  Pre-gravid obesity is associated with increased morbidity and mortality for both mother and offspring. Recent studies have demonstrated a heightened inflammatory response both systemically and locally within the adipose and placental tissue in women with pre-gravid obesity, which may play a role in mediating the adverse pregnancy outcomes. The aim of this study was to characterise the maternal and placental inflammatory status and investigate associated changes in placental structure in obese women.
  
  
  METHODS
  The pro-inflammatory status of a cohort of 47 non-obese (BMI 20-25 kg/m(2)) and 33 obese (≥30 kg/m(2)) women was characterised by measuring maternal circulating levels and placental gene expression of pro-inflammatory cytokines, and quantifying immune cell populations within the placenta. The effect of pre-gravid obesity on placental structure was investigated by examining placental maturity, vessel density, the formation of syncytial knots and sprouts, and the degree of fibrin deposition, chorangiosis and muscularisation of vessel walls.
  
  
  RESULTS
  Maternal obesity was associated with significantly greater IL-1β (p < 0.05), IL-8 (p < 0.05), MCP-1 (p < 0.001) and CXCR2 (p < 0.05) mRNA expression within the placenta and higher circulating maternal levels of IL-6 (3.30 ± 0.38 vs. 1.77 ± 0.15 pg/ml) (p < 0.001) compared with non-obese women. There were no differences in the number of CD14(+), CD68(+) cells or neutrophils within the placental villi of non-obese and obese women. However there were significantly higher numbers of neutrophils within the interstitial space (p < 0.05). Greater muscularity of placental vessel walls was associated with maternal obesity (p = 0.03), however no other associated structural changes were observed.
  
  
  CONCLUSIONS
  Our findings show that although pre-gravid obesity was associated with greater expression of placental pro-inflammatory cytokines and higher circulating IL-6 in pregnancy, there were no major differences in immune cell populations within the placental villi and only a greater degree of muscularity in the vessel walls.
authors.affiliation: University of Edinburgh, Queens Medical Research Institute, Little France Crescent, Edinburgh, UK. ka.roberts@ed.ac.uk
authors.forename: K A
authors.initials: KA
authors.lastname: Roberts
authors.forename: S C
authors.initials: SC
authors.lastname: Riley
authors.forename: R M
authors.initials: RM
authors.lastname: Reynolds
authors.forename: S
authors.initials: S
authors.lastname: Barr
authors.forename: M
authors.initials: M
authors.lastname: Evans
authors.forename: A
authors.initials: A
authors.lastname: Statham
authors.forename: K
authors.initials: K
authors.lastname: Hor
authors.forename: H N
authors.initials: HN
authors.lastname: Jabbour
authors.forename: J E
authors.initials: JE
authors.lastname: Norman
authors.forename: F C
authors.initials: FC
authors.lastname: Denison
mesh_terms: D000328:Adult; D002452:Cell Count; D015331:Cohort Studies; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D009765:Obesity / Q000276:immunology*; D010920:Placenta / Q000166:cytology / Q000276:immunology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
chemical_list: D016207:Cytokines; D012333:RNA, Messenger