title: OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro.
abstract: Research into the biology of the mammalian olfactory system would be greatly enhanced by transgenic reporter mice with cell-specific fluorescence. To this end we previously generated a mouse whose olfactory ensheathing cells (OECs) express DsRed driven by the S100ß promoter. We present here a transgenic reporter mouse whose olfactory sensory neurons express ZsGreen, driven by the olfactory marker protein (OMP) promoter. ZsGreen was very strongly expressed throughout the cytoplasm of olfactory sensory neurons labelling them in living cells and after fixation. Labelled sensory neurons were seen in all olfactory regions in the nose and fluorescent axons coursed through the lamina propria and into the main and accessory bulbs. We developed methods for culturing embryonic and postnatal olfactory sensory neurons using these mice to visualise living cells in vitro. ZsGreen was expressed along the length of axons providing exceptional detail of the growth cones. The ZsGreen fluorescence was very stable, without fading during frequent imaging. The combination of OMP-ZsGreen and S100ß-DsRed transgenic mice is ideal for developmental studies and neuron-glia assays and they can be bred with mutant mice to dissect the roles of various molecules in neurogenesis, differentiation, axon growth and targeting and other aspects of olfactory sensory neuron and glia biology.
authors.affiliation: National Centre for Adult Stem Cell Research, Eskitis Institute for Cell and Molecular Therapies, 170 Kessels Road, Griffith University, Nathan 4111, Brisbane, QLD, Australia.
authors.forename: Jenny A K
authors.initials: JA
authors.lastname: Ekberg
authors.forename: Daniel
authors.initials: D
authors.lastname: Amaya
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Chehrehasa
authors.forename: Katie
authors.initials: K
authors.lastname: Lineburg
authors.forename: Christina
authors.initials: C
authors.lastname: Claxton
authors.forename: Louisa C E
authors.initials: LC
authors.lastname: Windus
authors.forename: Brian
authors.initials: B
authors.lastname: Key
authors.forename: Alan
authors.initials: A
authors.lastname: Mackay-Sim
authors.forename: James A
authors.initials: JA
authors.lastname: St John
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000502:physiology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D051842:Olfactory Marker Protein / Q000235:genetics / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000166:cytology*; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000378:metabolism*
chemical_list: D017077:Culture Media, Conditioned; D008164:Luminescent Proteins; D051842:Olfactory Marker Protein; C497598:Omp protein, mouse; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins