title: [Effects of endostatin on the growth and lymphangiogenesis of Lewis lung carcinoma xenograft in mice].
abstract: OBJECTIVE
  to investigate the effects of endostar, a recombined humanized endostatin, on the growth, lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma xenograft in mice.
  
  
  METHODS
  lewis lung carcinoma (LLC) xenograft were established in C57 mice by intravenous transplantation of 1 × 10(6) cells. Then tumor-bearing mice were assigned into two groups: control group received caudal vein injections of 0.2 ml of 0.9% sodium chloride for 15 days, and treatment group received 500 µg endostar daily. Six weeks after LLC cell injection mice were sacrificed, and then tumor numbers and size were recorded. The expression of vascular endothelial growth factor-c (VEGF-C) and microlymphatic vessel density (MLVD) were observed by immunohistochemical staining.
  
  
  RESULTS
  tumor number and size of control group were significantly higher than those of treatment group. The microlymphatic vessel density (MLVD) was 5.7 ± 1.6 in the treatment group, which was markedly lower than in the control mice (7.8 ± 1.6). Two lymph node metastases were observed in treatment group, and eight in control group. Lymphatic metastases were more frequent in control group than in treatment group. Expression of VEGF-C in control group was significantly higher than that in treatment group.
  
  
  CONCLUSIONS
  endostar significantly inhibits the growth, lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma xenografts, and the inhibitory effect is due to its ability to regulate the expression of VEGF-C of tumors in part.
authors.affiliation: Department of Thoracic surgery, Second Hospital of Shandong University, Shandong University, Jinan 250033, China. dxp3260@sohu.com
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Dong
authors.forename: Tian-Hui
authors.initials: TH
authors.lastname: Xiao
authors.forename: Ling-Bai
authors.initials: LB
authors.lastname: Meng
authors.forename: Can-Hua
authors.initials: CH
authors.lastname: Yun
authors.forename: Hui
authors.initials: H
authors.lastname: Tian
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000473:pathology*; D043169:Endostatins / Q000494:pharmacology*; D042583:Lymphangiogenesis / Q000187:drug effects*; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D043169:Endostatins; D042582:Vascular Endothelial Growth Factor C; C467483:vascular endothelial growth factor C, mouse