title: Wnt inhibitors enhance chondrogenesis of human mesenchymal stem cells in a long-term pellet culture.
abstract: The long-term effects (~3 weeks) of two Wnt inhibitors (dickkopf [DKK]-1 and secreted frizzled-related protein [sFRP]-1), on the chondrogenic differentiation of human mesenchymal stem cells (hMSCs) was determined. Wnt inhibitors significantly increased the amount of glycosaminoglycan (GAG) in treated pellets (P<0.05). The gene expression of COL2A1 increased and COL1A1 decreased while the gene expression of SOX-9 and COL10A1 did not change significantly after three weeks of in vitro culture. The protein expression of type II collagen significantly increased (P<0.05) and that of type I collagen significantly decreased (P<0.05) while SOX-9 and type X collagen protein expression was unaffected. These findings suggest that Wnt inhibitors promote the chondrogenic differentiation of hMSCs when treated for three weeks.
authors.affiliation: Department of Orthopaedics, Dongguk University Ilsan Hospital, 814 Siksa-Dong, Goyang 411-773, Republic of Korea. gunil@duih.org
authors.forename: Gun-Il
authors.initials: GI
authors.lastname: Im
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Hye-Joung
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D020219:Chondrogenesis; D024042:Collagen Type I / Q000096:biosynthesis; D000090602:Collagen Type I, alpha 1 Chain; D024043:Collagen Type II / Q000096:biosynthesis; D024121:Collagen Type X / Q000096:biosynthesis; D020869:Gene Expression Profiling; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000502:physiology*; D055758:SOX9 Transcription Factor / Q000096:biosynthesis; D051153:Wnt Proteins / Q000037:antagonists & inhibitors*
chemical_list: C499177:COL2A1 protein, human; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D024043:Collagen Type II; D024121:Collagen Type X; D006025:Glycosaminoglycans; D055758:SOX9 Transcription Factor; D051153:Wnt Proteins