title: Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress.
abstract: The present study was undertaken to determine the role of glutathione peroxidase3 (gpx3) in phospholipid protection in cells. Wild-type (WT) cells showed an overall increase in phospholipids upon 50 μM cadmium (Cd)-treatment, whereas an untreated gpx3Δ strain showed a drastic reduction in overall phospholipids which was further reduced with 50 μM Cd. In WT cells, Cd-exposure increased the short chain fatty acids and decreased the unsaturated fatty acids and the magnitude was high in Cd-treated gpx3Δ cells. Purified recombinant gpx3p showed higher activity with phospholipid hydroperoxides than shorter hydroperoxides. An increase in gpx activity was observed in Cd-treated WT cells and no such alteration was observed in gpx3Δ. WT cells treated with Cd showed an increase in MDA over untreated, while untreated gpx3Δ cells themselves showed a higher level of MDA which was further enhanced with Cd-treatment. Iron, zinc and calcium levels were significantly altered in WT and gpx3Δ cells during Cd-treatment.
authors.affiliation: Department of Biochemistry, Bharathidasan University, Tiruchirappalli, 620 024, Tamilnadu, India.
authors.forename: Kannan
authors.initials: K
authors.lastname: Muthukumar
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Rajakumar
authors.forename: Mary Nirmala
authors.initials: MN
authors.lastname: Sarkar
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Nachiappan
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D010743:Phospholipids; D002104:Cadmium; D005979:Glutathione Peroxidase