title: Alkaline phosphatase (ALP) enhancing iridoid glucosides from the Indonesian medicinal plant Barleria lupulina.
abstract: In order to find antiosteoporotic agents from natural resources, 32 Indonesian medicinal plants were screened for their effects on osteoblast differentiation by using alkaline phosphatase (ALP) activity in MC3T3-E1 osteoblast cells as a marker. From the extract of Barleria lupulina, which showed the most potent activity, 13 iridoid glucosides, including three new ones [8-O-acetylipolamiidic acid (1), 8-O-acetyl-6-O-(p-methoxy-cis-cinnamoyl)shanzhiside (2), and 8-O-acetyl-6-O-(p-methoxy-trans-cinnamoyl)shanzhiside (3)] were identified. Among the 13 iridoid glucosides, ipolamiide (4) showed the most potent activity in a dose-dependent manner.
authors.affiliation: Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Retno
authors.initials: R
authors.lastname: Widyowati
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tezuka
authors.forename: Tatsurou
authors.initials: T
authors.lastname: Miyahara
authors.forename: Suresh
authors.initials: S
authors.lastname: Awale
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Kadota
mesh_terms: D016475:3T3 Cells; D027901:Acanthaceae / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D007214:Indonesia; D057888:Iridoid Glucosides / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D057888:Iridoid Glucosides; D000469:Alkaline Phosphatase