title: [Comparative genomic reconstruction of regulatory and metabolic networks in bacteria].
abstract: A large and growing number of complete genomes from diverse species open tremendous opportunities for getting deep insights into cell metabolism. This increased understanding strongly supports engineering of cell metabolism for microbial production. In spite of the recent progress, a large fraction of genes in most of the available genomes remain incorrectly or imprecisely annotated. In this paper we review some of the new comparative genomics techniques used to reconstruct regulatory and metabolic networks from genomic data, reveal gaps in current knowledge, and identify previously uncharacterized genes. The application will be discussed by using a recent example-reconstruction of xylose utilization pathway in Clostridium acetobutylicum.
authors.affiliation: Key Laboratory of Synthetic Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. chenyang@sibs.ac.cn
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000235:genetics* / Q000378:metabolism; D055028:Comparative Genomic Hybridization; D005818:Genetic Engineering; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D014994:Xylose / Q000378:metabolism*
chemical_list: D014994:Xylose