title: Hepatic gene expression profiling of 5'-AMP-induced hypometabolism in mice.
abstract: There is currently much interest in clinical applications of therapeutic hypothermia. Hypothermia can be a consequence of hypometabolism. We have recently established a procedure for the induction of a reversible deep hypometabolic state in mice using 5'-adenosine monophosphate (5'-AMP) in conjunction with moderate ambient temperature. The current study aims at investigating the impact of this technology at the gene expression level in a major metabolic organ, the liver. Our findings reveal that expression levels of the majority of genes in liver are not significantly altered by deep hypometabolism. However, among those affected by hypometabolism, more genes are differentially upregulated than downregulated both in a deep hypometabolic state and in the early arousal state. These altered gene expression levels during 5'-AMP induced hypometabolism are largely restored to normal levels within 2 days of the treatment. Our data also suggest that temporal control of circadian genes is largely stalled during deep hypometabolism.
authors.affiliation: Department of Biochemistry, UTHealth Medical School, Houston, Texas 77030, USA. Zhaoyang.Zhao@uth.tmc.edu
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Zhao
authors.forename: Takao
authors.initials: T
authors.lastname: Miki
authors.forename: Anita
authors.initials: A
authors.lastname: Van Oort-Jansen
authors.forename: Tomoko
authors.initials: T
authors.lastname: Matsumoto
authors.forename: David S
authors.initials: DS
authors.lastname: Loose
authors.forename: Cheng Chi
authors.initials: CC
authors.lastname: Lee
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D005260:Female; D007035:Hypothermia / Q000139:chemically induced / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013493:Suprachiasmatic Nucleus / Q000187:drug effects / Q000378:metabolism
chemical_list: D000249:Adenosine Monophosphate