title: Detection of synthetic RGDS(PO3H2)PA peptide adsorption using a titanium surface plasmon resonance biosensor.
abstract: The purpose of this study was to measure the time-dependent chemical interaction between synthetic RGDS(PO(3)H(2))PA (P-RGD) peptide and titanium surfaces using a titanium surface plasmon resonance (SPR) biosensor and to determine the degree of peptide immobilization on the surfaces. An SPR instrument for 'single-spot' analysis was used for nanometer-scale detection of biomolecular adsorption using a He-Ne laser light according to Knoll's method. The oxidized titanium surface was etched when exposed to H(3)PO(4) solutions with a pH of 2.0 or below. The amount of P-RGD adsorbed at pH 1.9 was approximately 3.6 times as much as that at pH 3.0 (P < 0.05). P-RGD naturally adsorbed on the oxidized titanium surface as a consequence of the bonding and dissociation mechanism of the phosphate functional group. Furthermore, the control of pH played a very important role in the interaction between P-RGD and the surface. These findings show that pH control may promote progressive binding of biomolecules with the phosphate functional group to the titanium surface.
authors.affiliation: Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. abey@hiroshima-u.ac.jp
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Abe
authors.forename: Kyou
authors.initials: K
authors.lastname: Hiasa
authors.forename: Isao
authors.initials: I
authors.lastname: Hirata
authors.forename: Yohei
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nogami
authors.forename: Wataru
authors.initials: W
authors.lastname: Mizumachi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okazaki
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Akagawa
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques; D006371:Helium; D006863:Hydrogen-Ion Concentration; D009356:Neon; D009842:Oligopeptides / Q000737:chemistry*; D063065:Organophosphonates / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; D063065:Organophosphonates; D010455:Peptides; D010710:Phosphates; D006371:Helium; D009356:Neon; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium; D010100:Oxygen