title: [A correlation analysis between the rate of vertical transmission of HBV and HBsAg-positive father to infant and the rate of neonatal cord blood HBV-DNA].
abstract: OBJECTIVE
  To study the influence of HBV-DNA with different load levels of HBsAg-positive among fathers on the rate of neonatal cord blood HBV-DNA.
  
  
  METHODS
  Using HBsAg and HBV-DNA as screening indicators for pregnant women and their husbands from an obstetric clinic. 161 pregnant women whose HBsAg and HBV-DNA were negative, but HBsAg was positive among their husbands and their newborns, were selected. Blood samples from those pregnant women, their husbands and their newborns were collected to detect the related indicators. Using ELISA to detect hepatitis B virus markers (HBVM), and FQ-PCR to detect the levels of HBV-DNA load. According to neonatal cord blood HBV-DNA detection guideline, newborns with cord blood HBV-DNA positive were selected as cases, others as controls.
  
  
  RESULTS
  (1) Result of the study showed that there was a dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive rates in newborns (trend χ(2) = 64.117, P = 0.000). The rate of vertical transmission of HBV from HBsAg-positive father to infant in the paternal serum HBV-DNA ≥ 1.0 × 10(7) copies/ml group was significantly higher than HBV-DNA < 1.0 × 10(7) copies/ml group (χ(2) = 71.539, P = 0.000). (2) There was a positive rank correlation between semen positive HBeAg and vertical transmission of HBV from HBsAg-positive father to infant (χ(2) = 6.892, P = 0.009).
  
  
  CONCLUSIONS
  There was a dose-response relationship between paternal serum HBV-DNA load levels and neonatal cord blood HBV-DNA positive in newborns. Paternal serum HBV-DNA ≥ 1.0 × 10(7) copies/ml and with HBeAg positive status were risk factors of vertical transmission of HBV from HBsAg-positive father to infant.
authors.affiliation: Fujian Provincial Maternal and Child Health Hospital, Fuzhou 350001, China. wpxzrl@163.com
authors.forename: Rong-lian
authors.initials: RL
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Jing-xian
authors.initials: JX
authors.lastname: Xie
authors.forename: Qi-yan
authors.initials: QY
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Cheng
authors.forename: Sheng-bin
authors.initials: SB
authors.lastname: Guo
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Huang
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D005220:Fathers; D005260:Female; D005312:Fetal Blood / Q000821:virology; D006509:Hepatitis B / Q000635:transmission*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D011247:Pregnancy; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens