title: [Expression of aquaporins and its significance in human pulmonary adenocarcinoma cell line SPC-A-1].
abstract: BACKGROUND
  To investigate the expression of aquaporins in human pulmonary adenocarcinoma cell line SPC-A-1.
  
  
  METHODS
  The expressions of aquaporin 1, aquaporin 3, aquaporin 4, and aquaporin 5 in mRNA level and their locations were determined in cell line SPC-A-1 respectively by RT-PCR and immunohistochemistry.
  
  
  RESULTS
  The immunohistochemical stain showed aquaporin 3 and aquaporin 5 located on the membrane of SPC-A-1 cell, but no positive stain of aquaporin 1 and aquaporin 4 was observed. Both aquaporin 3 and aquaporin 5 mRNA expressed in SPC-A-1 cell line, and the expression level of aquaporin 5 mRNA was significantly higher than that of aquaporin 3 mRNA ( P < 0.01). Aquaporin 1 and aquaporin 4 mRNA did not express in SPC-A-1 cell line.
  
  
  CONCLUSIONS
  Aquaporin 3 and aquaporin 5 express in SPC-A-1 cell, and their roles in water transport of SPC-A-1 cell should be further investigated.
authors.affiliation: Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R.China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Chunxue
authors.initials: C
authors.lastname: Bai
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Ren
authors.forename: Jie
authors.initials: J
authors.lastname: Hu