title: Plasma levels of n-decanoyl ghrelin, another acyl- and active-form of ghrelin, in human subjects and the effect of glucose- or meal-ingestion on its dynamics.
abstract: Besides n-octanoyl ghrelin (O-ghrelin), there is another acyl-form of ghrelin; n-decanoyl ghrelin (D-ghrelin), which has a decanoic acid modification. In this study, we examined the kinetics of D-ghrelin immunoreactivity in human plasma in comparison to O-ghrelin or total ghrelin by using a D-ghrelin-specific radioimmunoassay. The dynamics of plasma D-ghrelin was assessed following glucose- or meal-ingestion in healthy, non-obese subjects (5 males and 5 females). Correlations were also analyzed between the levels of plasma D-ghrelin and anthropometric or metabolic indicators in healthy human subjects (n=111, BMI 17.4-34.3). The plasma levels of D-ghrelin, like O- or T-ghrelin, significantly declined (p<0.05 for male and p<0.01 for female) 60 min after the ingestion of glucose in non-obese subjects. However, in the same subjects, no significant decline was noted in the levels of D-ghrelin, unlike O- or T-ghrelin, upon the meal ingestion. A significant increase was observed in the proportion of plasma D-ghrelin levels to that of T-ghrelin (p<0.05) in the healthy human subjects as BMI increased, unlike the proportion of O-ghrelin to T-ghrelin, which did not change. Since D-ghrelin possesses almost the same potential as that of O-ghrelin with regard to the feeding-stimulation, these differences between the dynamics of D- and O-ghrelin in human plasma might influence appetite-control, especially in those with increased BMI.
authors.affiliation: Department of Physiology, Kurume University School of Medicine, Kurume, 830-0011 Fukuoka, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Yoh
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tajiri
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamada
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yanase
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Doi
authors.forename: Koji
authors.initials: K
authors.lastname: Yonemoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kojima
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Tanaka
authors.forename: Jingo
authors.initials: J
authors.lastname: Kusukawa
mesh_terms: D000215:Acylation / Q000187:drug effects; D000217:Acyltransferases / Q000378:metabolism*; D000328:Adult; D000886:Anthropometry; D001069:Appetite Regulation; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D004435:Eating / Q000502:physiology; D005260:Female; D054439:Ghrelin / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D005947:Glucose / Q000008:administration & dosage; D006805:Human Experimentation; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood; D020033:Protein Isoforms / Q000097:blood* / Q000493:pharmacokinetics; D011863:Radioimmunoassay
chemical_list: D001786:Blood Glucose; D054439:Ghrelin; D007328:Insulin; D020033:Protein Isoforms; C422650:ghrelin, des-n-octanoyl; D000217:Acyltransferases; D005947:Glucose