title: Molecular characterization of three genes encoding aminopeptidases N in the poplar leaf beetle Chrysomela tremulae.
abstract: Three genes encoding proteins showing sequence similarity and features typical of insect APNs were characterized in C. tremulae and designed as CtAPN1, CtAPN2 and CtAPN3. Expression analysis of the three C. tremulae APN genes showed that CtAPN2 transcript is more abundant in the fat body, whereas both CtAPN1 and CtAPN3 are specifically expressed in the midgut. Despite a similar genomic organization, lepidopteran and coleopteran APNs are phylogenetically distant, suggesting that APN gene duplication events occurred after these two insect orders split. Sequence and expression comparisons of CtAPN1, CtAPN2 and CtAPN3 cDNAs in a C. tremulae Bacillus thuringiensis (Bt)-susceptible and in a Bt-resistant strain did not show any polymorphism at the amino acid level or difference at the transcription level.
authors.affiliation: INRA, UMR 1301 Interactions Biotiques et Santé Végétale, Sophia Antipolis, France. manuella.van.munster@supagro.inra.fr
authors.forename: M
authors.initials: M
authors.lastname: van Munster
authors.forename: M
authors.initials: M
authors.lastname: le Gleuher
authors.forename: Y
authors.initials: Y
authors.lastname: Pauchet
authors.forename: S
authors.initials: S
authors.lastname: Augustin
authors.forename: C
authors.initials: C
authors.lastname: Courtin
authors.forename: M
authors.initials: M
authors.lastname: Amichot
authors.forename: R H
authors.initials: RH
authors.lastname: Ffrench-Constant
authors.forename: D
authors.initials: D
authors.lastname: Pauron
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D018826:CD13 Antigens / Q000235:genetics*; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics*; D004064:Digestive System / Q000201:enzymology; D004731:Endotoxins; D005216:Fat Body / Q000201:enzymology; D005260:Female; D020869:Gene Expression Profiling; D006460:Hemolysin Proteins; D008018:Life Cycle Stages; D010572:Pest Control, Biological; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000235:genetics; D032107:Populus / Q000235:genetics; D016415:Sequence Alignment; D014231:Tribolium / Q000235:genetics
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D018826:CD13 Antigens