title: Protein-ligand binding investigated by a single nanoparticle TERS approach.
abstract: We report TERS imaging of individual 50 nm, biotin-labeled gold nanoparticles bound to a streptavidin-derivatized glass slide. Individual gold nanoparticles detected by a nanoparticle TERS tip generate Raman enhancements in both the biotin and streptavidin signals. These results indicate that nanoparticles are capable of investigating nanoscale spatial and chemical environments with non-resonant Raman enhancements.
authors.affiliation: University of Notre Dame, Department of Chemistry and Biochemistry, Notre Dame, IN, USA.
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Carrier
authors.forename: Corey M
authors.initials: CM
authors.lastname: Kownacki
authors.forename: Zachary D
authors.initials: ZD
authors.lastname: Schultz
mesh_terms: D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D008024:Ligands; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008024:Ligands; D001710:Biotin; D006046:Gold; D019809:Streptavidin