title: Human feeder cells can support the undifferentiated growth of human and mouse embryonic stem cells using their own basic fibroblast growth factors.
abstract: In the culture system using human feeder cells, the mechanism through which these cells support undifferentiated growth of embryonic stem cells (ESCs) has not been well investigated. Here, we explored the mechanisms of 3 kinds of human feeder cells, including human placental cells from the chorionic plate, human bone marrow stromal cells, and human foreskin fibroblasts. First, we determined that undifferentiated growth of 2 kinds each of human (H1 and HSF6) and mouse (D3 and CE3) ESCs was possible in all human feeder cell types tested (human placental cells, human bone marrow stromal cells, and human foreskin fibroblasts), without the need for exogenous cytokine supplementation including basic fibroblast growth factor (bFGF) and leukemia inhibitory factor. We then prepared their corresponding endogenous bFGF-knockout feeders using siRNA and tried to maintain human and mouse ESCs in their undifferentiated state; however, neither human nor mouse ESCs could be maintained in bFGF-knockout human feeder cells. The expressions of stemness markers such as Oct-4 and Nanog were significantly decreased in the bFGF-knockout group compared with those in the controls, and differentiation had already occurred, despite the undifferentiated morphologic appearance of the ESCs. In conclusion, human feeder cells are able to support the undifferentiated growth of human and mouse ESCs via bFGF synthesis. Further, a bFGF-dependent pathway might be crucial for maintaining the undifferentiated characteristics of mouse and human ESCs.
authors.affiliation: Institute of Stem Cell Research, Korea University, Seoul, Korea.
authors.forename: Yong
authors.initials: Y
authors.lastname: Park
authors.forename: Ji Hea
authors.initials: JH
authors.lastname: Kim
authors.forename: Seung Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: In Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Seh Jong
authors.initials: SJ
authors.lastname: Park
authors.forename: Se Ryeon
authors.initials: SR
authors.lastname: Lee
authors.forename: Hwa Jung
authors.initials: HJ
authors.lastname: Sung
authors.forename: Young Do
authors.initials: YD
authors.lastname: Yoo
authors.forename: Dong Ho
authors.initials: DH
authors.lastname: Geum
authors.forename: Chul Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Sun Haeng
authors.initials: SH
authors.lastname: Kim
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D052816:Foreskin / Q000166:cytology; D055786:Gene Knockout Techniques; D006801:Humans; D008297:Male; D051379:Mice; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D016222:Fibroblast Growth Factor 2