title: Macro-to-micro porous special bioactive glass and ceftriaxone-sulbactam composite drug delivery system for treatment of chronic osteomyelitis: an investigation through in vitro and in vivo animal trial.
abstract: A systematic and extensive approach incorporating in vitro and in vivo experimentation to treat chronic osteomyelitis in animal model were made using antibiotic loaded special bioactive glass porous scaffolds. After thorough characterization for porosity, distribution, surface charge, a novel drug composite were infiltrated by using vacuum infiltration and freeze-drying method which was subsequently analyzed by SEM-EDAX and studied for in vitro drug elution in PBS and SBF. Osteomyelitis in rabbit was induced by inoculation of Staphylococcus aureus and optimum drug-scaffold were checked for its efficacy over control and parenteral treated animals in terms of histopathology, radiology, in vivo drug concentration in bone and serum and implant-bone interface by SEM. It was optimized that 60P samples with 60-65% porosity (bimodal distribution of macro- to micropore) with average pore size ~60 μm and higher interconnectivity, moderately high antibiotic adsorption efficiency (~49%) was ideal. Results after 42 days showed antibiotic released higher than MIC against S. aureus compared to parenteral treatment (2 injections a day for 6 weeks). In vivo drug pharmacokinetics and SEM on bone-defect interface proved superiority of CFS loaded porous bioactive glass implants over parenteral group based on infection eradication and new bone formation.
authors.affiliation: Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, 196, Raja S. C. Mullick Road, Kolkata, 700028, India.
authors.forename: Biswanath
authors.initials: B
authors.lastname: Kundu
authors.forename: Samit Kumar
authors.initials: SK
authors.lastname: Nandi
authors.forename: Sudip
authors.initials: S
authors.lastname: Dasgupta
authors.forename: Someswar
authors.initials: S
authors.lastname: Datta
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Mukherjee
authors.forename: Subhasis
authors.initials: S
authors.lastname: Roy
authors.forename: Aruna Kumari
authors.initials: AK
authors.lastname: Singh
authors.forename: Tapan Kumar
authors.initials: TK
authors.lastname: Mandal
authors.forename: Partha
authors.initials: P
authors.lastname: Das
authors.forename: Rupnarayan
authors.initials: R
authors.lastname: Bhattacharya
authors.forename: Debabrata
authors.initials: D
authors.lastname: Basu
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001842:Bone and Bones / Q000187:drug effects; D002443:Ceftriaxone / Q000008:administration & dosage*; D002908:Chronic Disease; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D005898:Glass; D006863:Hydrogen-Ion Concentration; D010019:Osteomyelitis / Q000188:drug therapy* / Q000473:pathology; D016062:Porosity; D011208:Powders; D011817:Rabbits; D013211:Staphylococcus aureus / Q000378:metabolism; D013407:Sulbactam / Q000008:administration & dosage*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D011208:Powders; D002443:Ceftriaxone; D013407:Sulbactam