title: Effect of exogenous galectin-1 on leukocyte migration: modulation of cytokine levels and adhesion molecules.
abstract: The effect of exogenous Gal-1 on cellular response and adhesion molecule expression was investigated in a classical model of acute inflammation induced by zymosan. C57BL6 mice, treated or not with human recombinant (hr) Gal-1, received i.p. injection of zymosan and peritoneal exudate, blood and mesentery were processed for cellular, biochemical, light and electron microscopic analysis after 4 and 24 h. Zymosan peritonitis provoked the expected signs of inflammation at 4 h, including a significant increase in extravasated PMNs in the mesentery and peritoneal exudate, mirrored by blood neutrophilia. These changes subsided after 24 h. Ultrastructural immunocytochemical analysis of PMNs showed significant Gal-1 expression and co-localization with L-selectin and β2-integrin in the plasma membrane and cytoplasm. Pharmacological treatment with hrGal-1 at 4 h produced an inhibition of PMN migration, associated with diminished expression of adhesion molecules, particularly β2-integrin, and TNF-α and IL-1β release by peritoneal cells. At 24 h, Gal-1 induced an increase in mononuclear phagocytic cell recruitment. In conclusion, our data propose an important mechanism of anti-inflammatory action of Gal-1, initially by modulation of pro-inflammatory cytokine release and PMN migration through an imbalance between adhesion molecule expression and, later, by promoting monocyte-macrophage recruitment.
authors.affiliation: Department of Anatomy, São José do Rio Preto School of Medicine – FAMERP, São José do Rio Preto, SP, Brazil. cristiane.gil@unifesp.br
authors.forename: Cristiane D
authors.initials: CD
authors.lastname: Gil
authors.forename: Caio E
authors.initials: CE
authors.lastname: Gullo
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Oliani
mesh_terms: D000818:Animals; D018821:CD18 Antigens / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D054441:Cell Migration Assays, Leukocyte; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000737:chemistry / Q000378:metabolism; D037483:Galectin 1 / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008643:Mesentery / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010538:Peritonitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D011994:Recombinant Proteins
chemical_list: D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016207:Cytokines; D037483:Galectin 1; C445499:LGALS1 protein, human; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin