title: Globin-mediated nitric oxide detoxification in the foodborne pathogenic bacterium Campylobacter jejuni proceeds via a dioxygenase or denitrosylase mechanism.
abstract: Nitric oxide (NO(·)) is a toxin, but bacteria have evolved various strategies to detoxify this harmful radical to nitrate, the best known mechanism being the dioxygenase reaction of bacterial flavohaemoglobins. In addition, globins can form oxoferryl (Fe(IV)=O) species through the reaction of the ferric haem with hydrogen peroxide: these species can also detoxify NO(·) to nitrite and nitrate. During infection, Campylobacter is exposed to both NO(·) and hydrogen peroxide. A question therefore arises: does Campylobacter jejuni utilize its single domain globin (Cgb) to detoxify NO(·) via the oxoferryl route, or via the more conventional dioxygenase or denitroxylase routes? The data herein demonstrate that the reaction between Cgb and hydrogen peroxide is much slower than for other globins, and subsequent reaction between the oxoferryl species and NO(·) is unfavourable. Furthermore, NO(·) may bind to Cgb in the oxyferrous, ferrous and ferric states. The ample opportunity for NO(·) to interact with ferrous and ferric Cgb, and the unfavourable reaction of ferric Cgb with hydrogen peroxide, suggests that NO(·) detoxification in C. jejuni proceeds via a dioxygenase or denitroxylase route requiring the haem iron to exist only in the Fe(II) or Fe(III) redox states.
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia. m.shepherd1@uq.edu.au
authors.forename: Mark
authors.initials: M
authors.lastname: Shepherd
authors.forename: Paul V
authors.initials: PV
authors.lastname: Bernhardt
authors.forename: Robert K
authors.initials: RK
authors.lastname: Poole
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D016123:Campylobacter jejuni / Q000378:metabolism*; D049308:Dioxygenases / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D005914:Globins / Q000302:isolation & purification / Q000378:metabolism*; D006420:Hemeproteins / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D011485:Protein Binding; D013053:Spectrophotometry / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D005290:Ferric Compounds; D005296:Ferrous Compounds; D006420:Hemeproteins; D009569:Nitric Oxide; D005914:Globins; D006861:Hydrogen Peroxide; D010105:Oxygenases; D049308:Dioxygenases; C430724:flavohemoglobin denitrosylase; D010100:Oxygen