title: Methods for microRNA microarray profiling.
abstract: MicroRNAs (miRNAs) are single-stranded, small RNA molecules of 21-23 nucleotides that are primarily involved in the regulation of gene expression. A number of recent studies have shown that miRNAs play a vital role in signaling pathways important for human oncogenesis and may hold promise as potential biomarkers for cancer diagnosis, prognosis, and therapeutics. Microarray-based expression analysis is an emerging and powerful strategy for initially identifying candidate miRNAs, which can then be correlated to specific biological process such as carcinogenesis, and eventually developed as a molecular signature for a disease state. This chapter presents a protocol for miRNA microarray profiling using the Agilent platform, which is one of the most widely utilized technologies currently available.
authors.affiliation: Translational Genomics Research Institute, Phoenix, AZ, USA.
authors.forename: Aarati R
authors.initials: AR
authors.lastname: Ranade
authors.forename: Glen J
authors.initials: GJ
authors.lastname: Weiss
mesh_terms: D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D035683:MicroRNAs; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D035683:MicroRNAs