title: A novel bioactive tyramine derived Schiff base and its transition metal complexes as selective DNA binding agents.
abstract: A novel tyramine derived Schiff base, 3-4-dimethoxybenzylidene-4-aminoantipyrinyl-4-aminoethylphenol(L) and a series of its transition metal complexes of the type, ML2Cl2 where, M=Cu(II), Ni(II), Co(II) and Zn(II) have been designed and synthesized. Their structural features and other properties were deduced from the elemental analysis, magnetic susceptibility and molar conductivity as well as from mass, IR, UV-vis, 1H NMR and EPR spectral studies. The binding properties of these complexes with calf thymus DNA (CT-DNA) were investigated using electronic absorption spectroscopy, viscosity measurement, cyclic voltammetry and molecular docking analysis. The results reveal that the metal(II) complexes interact with DNA through minor groove binding. The interaction has also been investigated by gel electrophoresis. Interestingly, it was found that all the complexes could cleave the circular plasmid pUC19 super coiled (SC) DNA efficiently in the presence of AH2 (ascorbic acid). The complexes showed enhanced antifungal and antibacterial activities compared to the free ligand.
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar, Tamil Nadu 626 001, India. drn_raman@yahoo.co.in
authors.forename: N
authors.initials: N
authors.lastname: Raman
authors.forename: S
authors.initials: S
authors.lastname: Sobha
authors.forename: A
authors.initials: A
authors.lastname: Thamaraichelvan
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004553:Electric Conductivity; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D012545:Schiff Bases / Q000737:chemistry* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D017186:Titrimetry; D028561:Transition Elements / Q000737:chemistry* / Q000494:pharmacology; D014439:Tyramine / Q000737:chemistry* / Q000494:pharmacology; D014783:Viscosity / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D012545:Schiff Bases; D028561:Transition Elements; D004247:DNA; C427203:calf thymus DNA; D014439:Tyramine