title: Carbohydrate gel electrophoresis.
abstract: Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of the reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation of enzyme specificity.
authors.affiliation: Bayer BioScience NV, Ghent, Belgium. Florence.Goubet@bayercropscience.com
authors.forename: Florence
authors.initials: F
authors.lastname: Goubet
authors.forename: Paul
authors.initials: P
authors.lastname: Dupree
authors.forename: Katja Salomon
authors.initials: KS
authors.lastname: Johansen
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002351:Carrageenan / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D013058:Mass Spectrometry; D008970:Molecular Weight; D009281:Naphthalenes; D013194:Staining and Labeling
chemical_list: D002241:Carbohydrates; D009281:Naphthalenes; D002351:Carrageenan; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid