title: Hexameric architecture of CstF supported by CstF-50 homodimerization domain structure.
abstract: The Cleavage stimulation Factor (CstF) complex is composed of three subunits and is essential for pre-mRNA 3'-end processing. CstF recognizes U and G/U-rich cis-acting RNA sequence elements and helps stabilize the Cleavage and Polyadenylation Specificity Factor (CPSF) at the polyadenylation site as required for productive RNA cleavage. Here, we describe the crystal structure of the N-terminal domain of Drosophila CstF-50 subunit. It forms a compact homodimer that exposes two geometrically opposite, identical, and conserved surfaces that may serve as binding platform. Together with previous data on the structure of CstF-77, homodimerization of CstF-50 N-terminal domain supports the model in which the functional state of CstF is a heterohexamer.
authors.affiliation: Institut Européen de Chimie et Biologie, Institut National de la Santé et de la Recherche Médicale (INSERM) U869, Pessac, France.
authors.forename: María
authors.initials: M
authors.lastname: Moreno-Morcillo
authors.forename: Lionel
authors.initials: L
authors.lastname: Minvielle-Sébastia
authors.forename: Cameron
authors.initials: C
authors.lastname: Mackereth
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fribourg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039223:Cleavage And Polyadenylation Specificity Factor / Q000737:chemistry* / Q000378:metabolism*; D039241:Cleavage Stimulation Factor / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D026723:Polyadenylation; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D039223:Cleavage And Polyadenylation Specificity Factor; D039241:Cleavage Stimulation Factor; D012322:RNA Precursors