title: Anti-diabetic effects of a Coptis chinensis containing new traditional Chinese medicine formula in type 2 diabetic rats.
abstract: The Chinese formula Tang-Min-Ling (TML), an improved product of the decoction of Dachaihu which has a history of more than 2000 years, has main constituents of Coptis chinensis Franch, Scutellaria baicalensis Georgi, Rheum officinale Baill and Bupleurum chinense DC. A multi-central randomized controlled investigation performed previously by us has showed that TML has positive effects on regulating glycometabolism in type 2 diabetes (T2DM) patients, but the mechanisms remain unclear. Using Otsuka Long-Evans Tokushima Fatty (OLETF) rats as an animal model with rosiglitazone as a positive control, we were able to detect TML's effect on the serum glucose, serum lipid, serum leptin and adiponcetin after oral administration for 12 weeks. We were also able to detect the insulin resistance level by a glucose clamp test and study the mechanisms of TML in improving insulin resistance by detecting skeletal muscle AMP-activated protein kinase (AMPK) and glucose transporter 4 (GLUT4). Results showed that TML significantly reduced the glucose area under a curve of the oral glucose tolerance test, and had a positive effect in regulating serum lipid metabolism. TML treatment also significantly reduced the serum leptin level, but it had no effect on the serum adiponectin level. The AMPK enzymatic activity and GLUT4 expression in Skeletal Muscle were also upregulated in the TML group. The results suggest that the Chinese medicine TML, which contains Coptis chinensis Franch as one of its components, improves glycometabolism and its possible mechanisms may involve in improvement of insulin resistance of OLETF rats.
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P. R. China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhen
authors.forename: Bai
authors.initials: B
authors.lastname: Chang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Feng-Mei
authors.initials: FM
authors.lastname: Lian
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Liu
authors.initials: L
authors.lastname: Dong
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Wen-Ke
authors.initials: WK
authors.lastname: Liu
authors.forename: Xiu-Yang
authors.initials: XY
authors.lastname: Li
authors.forename: Pei-Jie
authors.initials: PJ
authors.lastname: Qin
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Tong
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D052242:Adiponectin / Q000097:blood; D000818:Animals; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism; D029972:Bupleurum / Q000737:chemistry; D039323:Coptis / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000628:therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005951:Glucose Tolerance Test; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D006949:Hyperlipidemias / Q000097:blood / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007333:Insulin Resistance; D020738:Leptin / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D018482:Muscle, Skeletal / Q000378:metabolism; D008517:Phytotherapy; D051381:Rats; D020334:Rats, Inbred OLETF; D012250:Rheum / Q000737:chemistry; D000077154:Rosiglitazone; D031364:Scutellaria baicalensis / Q000737:chemistry; D045162:Thiazolidinediones / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D020738:Leptin; D008055:Lipids; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D055372:AMP-Activated Protein Kinases