title: Discrimination of two picarel species Spicara flexuosa and Spicara maena (Pisces: Centracanthidae) based on mitochondrial DNA sequences.
abstract: A DNA methodology based on PCR and sequencing analysis of the mtDNA 16S rRNA gene was developed for the discrimination of picarel Spicara flexuosa and blotched picarel Spicara maena (Pisces: Centracanthidae). The molecular results indicated that there is a clear discrimination between the two species, as all the individuals of S. flexuosa revealed the same 16S rDNA haplotype while the S. maena haplotype differs in 15 distinct nucleotides.
authors.affiliation: Alexander Technological Educational Institute of Thessaloniki, Department of Aquaculture & Fisheries Technology, P. O. Box 157, GR-63200, Nea Moudania, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Imsiridou
authors.forename: G
authors.initials: G
authors.lastname: Minos
authors.forename: A
authors.initials: A
authors.lastname: Gakopoulou
authors.forename: V
authors.initials: V
authors.lastname: Katsares
authors.forename: T
authors.initials: T
authors.lastname: Karidas
authors.forename: G
authors.initials: G
authors.lastname: Katselis
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D006239:Haplotypes; D010473:Perciformes / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S