title: Polymorphisms in the CD36/FAT gene are associated with plasma vitamin E concentrations in humans.
abstract: BACKGROUND
  Blood vitamin E concentrations are modulated by dietary, metabolic, and genetic factors. CD36 (cluster of differentiation 36), a class B scavenger receptor, might be involved in tissue vitamin E uptake and thus would influence blood vitamin E concentrations.
  
  
  OBJECTIVE
  The goal of the study was to assess the association between CD36 single nucleotide polymorphisms (SNPs) and plasma α-tocopherol concentrations in humans.
  
  
  METHODS
  A subsample from the adult SU.VI.MAX (SUpplementation en VItamines et Minéraux AntioXydants) cohort (n = 621) and the adolescent cross-sectional HELENA (Healthy Lifestyle in Europe by Nutrition in Adolescence) Study (n = 993) were genotyped for CD36 SNPs (4 and 10 SNPs, respectively). Fasting plasma α-tocopherol concentrations were assayed by using HPLC. Associations were determined by haplotype analyses and by general linear regression models.
  
  
  RESULTS
  In the SU.VI.MAX subsample, haplotype analyses showed that some haplotypes of SNPs rs1984112, rs1527479, rs7755, and rs1527483 tended to be associated with plasma α-tocopherol concentrations (P = 0.08 and P = 0.09 for haplotypes 1222 and 1122, respectively). We then investigated the whole known common genetic variability (10 SNPs) of CD36 in the HELENA Study. Three SNPs were associated with lower plasma α-tocopherol concentrations (rs1984112: -3.2%, P = 0.053; rs1761667: -2.9%, P = 0.046; rs1527479: -3.7%, P = 0.0061). After correction for multiple testing, the association between rs1527479 and α-tocopherol concentrations remained significant. This association was modulated by concentrations of fasting serum triglycerides (P for interaction = 0.006) and long-chain polyunsaturated fatty acids (P for interaction = 0.005).
  
  
  CONCLUSIONS
  Our results suggest that CD36 can modulate blood α-tocopherol concentrations and may therefore be involved in the intestinal absorption or tissue uptake of vitamin E.
authors.affiliation: INSERM, U744, Institut Pasteur de Lille, Université Lille Nord de France, Lille, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lecompte
authors.forename: Fabien
authors.initials: F
authors.lastname: Szabo de Edelenyi
authors.forename: Louisa
authors.initials: L
authors.lastname: Goumidi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maiani
authors.forename: George
authors.initials: G
authors.lastname: Moschonis
authors.forename: Kurt
authors.initials: K
authors.lastname: Widhalm
authors.forename: Dénes
authors.initials: D
authors.lastname: Molnár
authors.forename: Anthony
authors.initials: A
authors.lastname: Kafatos
authors.forename: Andre
authors.initials: A
authors.lastname: Spinneker
authors.forename: Christina
authors.initials: C
authors.lastname: Breidenassel
authors.forename: Jean
authors.initials: J
authors.lastname: Dallongeville
authors.forename: Aline
authors.initials: A
authors.lastname: Meirhaeghe
authors.forename: Patrick
authors.initials: P
authors.lastname: Borel
mesh_terms: D000293:Adolescent; D000328:Adult; D018955:CD36 Antigens / Q000235:genetics*; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D005060:Europe; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D005602:France; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000097:blood*; D055815:Young Adult; D024502:alpha-Tocopherol / Q000097:blood
chemical_list: D018955:CD36 Antigens; D005231:Fatty Acids, Unsaturated; D014280:Triglycerides; D014810:Vitamin E; D024502:alpha-Tocopherol