title: Isotypic neutralizing antibodies against mouse GCP-2/CXCL6 inhibit melanoma growth and metastasis.
abstract: The chemokine granulocyte chemotactic protein (GCP)-2/CXCL6 promotes tumor growth as angiogenesis inducer and neutrophil chemoattractant. The neutralizing capacity and specificity of monoclonal mouse anti-murine (mu)GCP-2/CXCL6 antibodies were evidenced by granulocyte chemotaxis and signaling assays. The half-life of the non-antigenic antibody in the blood circulation was approximately 15 days. The titers remained constant upon weekly injection. Tumor growth and lymphogenic metastases of human melanoma over-expressing muGCP-2 were reduced in mice treated with anti-muGCP-2. Moreover, the drop in muGCP-2 antibody titer correlated with the melanoma tumor size. Taken together, we show that functional blocking of GCP-2 inhibits tumor growth and metastases.
authors.affiliation: Rega Institute for Medical Research, Laboratory of Molecular Immunology, K.U.Leuven, Leuven, Belgium.
authors.forename: Hannelien
authors.initials: H
authors.lastname: Verbeke
authors.forename: Sofie
authors.initials: S
authors.lastname: Struyf
authors.forename: Nele
authors.initials: N
authors.lastname: Berghmans
authors.forename: Els
authors.initials: E
authors.lastname: Van Coillie
authors.forename: Ghislain
authors.initials: G
authors.lastname: Opdenakker
authors.forename: Catherine
authors.initials: C
authors.lastname: Uyttenhove
authors.forename: Jacques
authors.initials: J
authors.lastname: Van Snick
authors.forename: Jo
authors.initials: J
authors.lastname: Van Damme
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics / Q000494:pharmacology; D057134:Antibodies, Neutralizing / Q000276:immunology / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D054427:Chemokine CXCL6 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D023041:Xenograft Model Antitumor Assays
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D054427:Chemokine CXCL6