title: Culture and identification of human amniotic mesenchymal stem cells.
abstract: OBJECTIVE
  To establish the method of isolation, purification, and identification of human amniotic mesenchymal stem cells (hAMSCs).
  
  
  METHODS
  hAMSCs were isolated from human amniotic membrane by trypsin-collagenase digestion, and cultured in Dulbecco's modified Eagle's medium/F12 medium supplemented with 10% fetal bovine serum. Phenotypic characteristics of these cells were analyzed by means of immunocytochemistry and flow cytometry.
  
  
  RESULTS
  The cells successfully isolated from human amniotic membrane expressed representative mesenchymal cell surface markers CD44, CD90, and vimentin, but not CD45.
  
  
  CONCLUSIONS
  This study establishes a potential method for isolation of hAMSCs from human amnion,in vitro culture, and identification. The isolated cells show phenotypic characteristics of mesenchymal stem cells.
authors.affiliation: Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang 110001, China.
authors.forename: Huo
authors.initials: H
authors.lastname: Shuang-zhi
authors.forename: Shi
authors.initials: S
authors.lastname: Ping
authors.forename: Pang
authors.initials: P
authors.lastname: Xi-ning
mesh_terms: D000650:Amnion / Q000166:cytology*; D018929:Cell Culture Techniques; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*