title: Construction and immunogenicity of a recombinant pseudotype baculovirus expressing the glycoprotein of rabies virus in mice.
abstract: A pseudotype baculovirus with the glycoprotein of vesicular stomatitis virus (VSV-G) on the envelope was used as a vector for the construction of recombinant baculovirus expressing the G protein of rabies virus (RABV) under the cytomegalovirus (CMV) promoter. The generated recombinant baculovirus (BV-G) efficiently expressed the RABV G proteins in mammalian cells. Intramuscular vaccination with BV-G (10(9) PFU/mouse) induced the production of RABV G-specific neutralizing antibodies and strong T cell responses in mice. Our data clearly indicate that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop a new generation of vaccine against RABV infection.
authors.affiliation: College of Animal Science and Veterinary Medicine, Agricultural Division, Jilin University, Changchun, People's Republic of China.
authors.forename: Hainan
authors.initials: H
authors.lastname: Huang
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
authors.forename: Junling
authors.initials: J
authors.lastname: Qin
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Jiang
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.forename: Zilong
authors.initials: Z
authors.lastname: Li
authors.forename: Tiansong
authors.initials: T
authors.lastname: Li
authors.forename: Xiuchan
authors.initials: X
authors.lastname: Su
authors.forename: Yang
authors.initials: Y
authors.lastname: Ruan
authors.forename: Fengqin
authors.initials: F
authors.lastname: Xu
authors.forename: Hualei
authors.initials: H
authors.lastname: Wang
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics*; D004337:Drug Carriers; D005260:Female; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice; D008813:Mice, Inbred ICR; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D018116:Vesiculovirus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004337:Drug Carriers; D006023:Glycoproteins; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus