title: [First outbreak report of VIM-1 metallo-beta-lactamase producing Pseudomonas aeruginosa in Japan].
abstract: VIM-1 metallo-beta-lactamase (MBL) producing Pseudomonas aeruginosa was isolated from 35 Kobe City Medical Center General Hospital patients from September 2007 to July 2008. All but one were highly resistant to all beta-lactams, aminoglycoside, and fluoroquinolone, and one susceptible to amikacin. Strains negative to a disk diffusion screening test using sodium mercaptoacetate for detecting MBL numbered 35. PCR for MBL indicated all strains were positive for bla(VIWM-1). These strains were indistinguishable by pulsed-field gel electrophoresis, indicating an outbreak of infections caused by VIM-1 MBL producing Pseudomonas aeruginosa. After intervention to control contact, the outbreak was controlled.
authors.affiliation: Department of Clinical Laboratory, Kobe City Medical Center General Hospital.
authors.forename: Kanji
authors.initials: K
authors.lastname: Miki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takegawa
authors.forename: Masaaki
authors.initials: M
authors.lastname: Etoh
authors.forename: Michio
authors.initials: M
authors.lastname: Hayashi
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Haruta
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Yamane
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000583:Amikacin / Q000494:pharmacology; D000617:Aminoglycosides / Q000494:pharmacology; D001431:Bacteriological Techniques; D004196:Disease Outbreaks; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D011552:Pseudomonas Infections / Q000453:epidemiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis
chemical_list: D000617:Aminoglycosides; D024841:Fluoroquinolones; D000583:Amikacin; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases