title: A novel population of mesenchymal progenitors with hematopoietic potential originated from CD14 peripheral blood mononuclear cells.
abstract: Hemopoietic system derived progenitor cells with mesenchymal features have been identified including CD14(+) monocyte-derived progenitors. However, it is unclear whether there are mesenchyme derived progenitors with hematopoietic potential. Herein, we identified a novel CD14(-) cell-derived population with both mesenchymal and hematopoietic features in rat peripheral blood, and this cell population is different from the CD14(+) monocyte-derived progenitors but designated peripheral blood multipotential mesenchymal progenitors (PBMMPs). Phenotype analysis demonstrated expression of mesenchymal markers in PBMMPs including BMPRs, Endoglin/CD105, Fibronectin (Fn), Vimentin (Vim), Collagen (Col) I/II/III along with hematopoietic marker CD34. CD14(+) cell-derived population shared the same characteristics with CFs. In mixed culture of CD14(+) and CD14(-) cells, PBMMPs were a predominant component and expressed CD29(high), CD73(high), CD34(high), CD45(low) and CD90. Except for the value of mixed T lymphocytes and CD14(+) cell-derived population, hematopoietic characters of cultured PBMMPs were indicated by CD14(-)/CD34(+)/CD45(-)/CD90(+). The mesenchymal origin was further confirmed by comparing PBMMPs with bone marrow stromal cells. Finally, we transplanted PBMMPs into a skin wound model, and results showed the specific potential of PBMMPs in not only extracellular matrix secretion but epidermal regeneration. This study provides evidence that peripheral blood contains common hematopoietic-mesenchymal progenitors from both hematopoietic and mesenchymal lineages, and CD34(+) mesenchymal progenitors are a possible alternative source of epidermal cells in wound healing.
authors.affiliation: Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000276:immunology*; D003094:Collagen / Q000378:metabolism; D006410:Hematopoiesis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D036102:Peripheral Blood Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D018952:Antigens, CD34; D018950:Lipopolysaccharide Receptors; D003094:Collagen