title: Time-dependent modulation of serotonin and its receptors 1A and 2A expression in allergic contact dermatitis.
abstract: BACKGROUND
  Serotonin implication in allergic contact dermatitis was earlier suggested.
  
  
  OBJECTIVE
  To study the expression of serotonin (5-HT) and its receptors 1A and 2A during the kinetics of allergic contact dermatitis (ACD).
  
  
  METHODS
  Biopsies from 10 female nickel-allergic patients were obtained at 0, 6, 24, 48 and 72 h after nickel application using a patch testing procedure and then analysed by immunohistochemistry.
  
  
  RESULTS
  Higher 5-HT epidermal immunoreactivity was seen at 0 and 6 h compared with 24, 48 and 72 h. The number of 5-HT labelled platelets was higher at 48 and 72 h compared with 0, 6 and 24 h. There was a decrease in the number of 5-HT1AR positive cells at 24, 48 and 72 h compared with 0 h. Furthermore, epidermal 5-HT1AR immunoreactivity was stronger at 48 and 72 h compared with 0 h. Total (dermis plus epidermis) number of 5-HT2AR positive cells was gradually increased in a time-dependent manner at 6, 24, 48 and 72 h compared with 0 h.
  
  
  CONCLUSIONS
  Our results suggest the implication of 5-HT, 5-HT1AR and 5-HT2AR in the development of human ACD and the possibility to target those receptors at an early stage of this inflammatory condition.
authors.affiliation: Dermatology and Venereology Unit, Department of Medicine, Solna, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Wetterberg
authors.forename: C
authors.initials: C
authors.lastname: Taher
authors.forename: E C
authors.initials: EC
authors.lastname: Azmitia
authors.forename: H
authors.initials: H
authors.lastname: El-Nour
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D017449:Dermatitis, Allergic Contact / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009532:Nickel / Q000009:adverse effects; D010328:Patch Tests; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism*; D044402:Receptor, Serotonin, 5-HT2A / Q000378:metabolism*; D012189:Retrospective Studies; D012701:Serotonin / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D044402:Receptor, Serotonin, 5-HT2A; D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin; D009532:Nickel