title: Full genome sequence and virulence analyses of the recent equine isolate of Japanese encephalitis virus.
abstract: In the past 25 years, there has been only one case of Japanese encephalitis in horses in Japan. We determined the full genome sequence of the Japanese encephalitis virus (JEV) strain JEV/eq/Tottori/2003 isolated from an afflicted horse and also analyzed its virulence in mice. The sequence analysis showed that the genome of JEV/eq/Tottori/2003 is similar to that of genotype I, a dominant genotype of JEV presently circulating in Japan. Its neurovirulence, but not neuroinvasiveness, was still as high as it was for genotype III, thus indicating the necessity for continuation of a vaccination program of horses against JEV.
authors.affiliation: Laboratory of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Nagao
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Seiji
authors.initials: S
authors.lastname: Tamaru
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamanaka
authors.forename: Tomio
authors.initials: T
authors.lastname: Matsumura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001681:Biological Assay / Q000662:veterinary; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000472:pathogenicity; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D005838:Genotype; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D007564:Japan; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014774:Virulence
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins