title: Respiratory competent mitochondria in human ovarian and peritoneal cancer.
abstract: Impaired respiration was proposed by Warburg to be responsible for aerobic glycolysis in cancer cells. However, intact mitochondria isolated from human ovarian and peritoneal cancer tissues exhibit substantive oxidative phosphorylating activities in terms of membrane potential, ATP biosynthesis and oxygen consumption. The specific activities of succinate, malate and glutamate dehydrogenases are comparable to reported values for human skeletal muscle, heart and liver but the rate of ATP production is one order of magnitude lower compared to human skeletal muscle. It was concluded that the TCA cycle is functional in these ovarian cancer tissues which contain OXPHOS competent mitochondria.
authors.affiliation: Departments of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore. bchlhy@nus.edu.sg
authors.forename: Hwee Ying
authors.initials: HY
authors.lastname: Lim
authors.forename: Qin Shi
authors.initials: QS
authors.lastname: Ho
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Low
authors.forename: Mahesh
authors.initials: M
authors.lastname: Choolani
authors.forename: Kim Ping
authors.initials: KP
authors.lastname: Wong
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D000818:Animals; D019069:Cell Respiration; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006801:Humans; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial; D051379:Mice; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D010051:Ovarian Neoplasms / Q000503:physiopathology*; D010085:Oxidative Phosphorylation; D010100:Oxygen / Q000378:metabolism; D010534:Peritoneal Neoplasms / Q000503:physiopathology*; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D005969:Glutamate Dehydrogenase; D010100:Oxygen