title: Characterization of 3' termini of human cytomegalovirus UL138-UL145 transcripts in a clinical strain.
abstract: The functions of some proteins encoded by human cytomegalovirus (HCMV) UL/b' genes have been studied; however, systematic analysis of the transcripts for this region is still insufficient. The results of both rapid amplification of cDNA ends (RACE) and cDNA library screening in this study proved that 3' termini of all transcripts in the UL138-UL145 region were located approximately 20 bp downstream from each potential poly (A) signal, which were at the positions of nucleotides 7184, 9954 and 12848 in the UL/b' sequence of the H strain, respectively. Thus, there were at least two large families of polycistronic transcripts in this gene region. The first family of 3'-coterminal transcripts contained UL139, UL140 and UL141 genes, and the second one consisted of UL142, UL143, UL144 and UL145 genes. The 3'-coterminal characterization further confirmed that multiple uses of polyadenylation signals were commonly used by HCMV to utilize genetic information.
authors.affiliation: Virus Laboratory, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning 110004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Ji
authors.forename: Mali
authors.initials: M
authors.lastname: Li
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.forename: Gaowei
authors.initials: G
authors.lastname: Ren
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D014764:Viral Proteins