title: [Influence of acupuncture on the motor ability in training-induced fatigue mice].
abstract: OBJECTIVE
  To observe the influence of acupuncture on the motor ability of training-induced fatigue mice so as to explore its mechanism underlying acupuncture-induced improvement of physical training fatigue.
  
  
  METHODS
  Sixty Kunming mice were divided into normal control, training model and acupuncture groups, with 20 mice in each. The training-fatigue model was established by forcing the mouse to swim in a water tank for 30 min/d in the 1st week, 60 min/d in the 2nd week, 90 min/d in the 3rd week and 120 min/d in the 4th and 5th weeks. "Guanyuan" (CV 4), and bilateral "Zusanli" (ST 36) and "Shenshu" (BL 23) were punctured with filiform needles, and with the needle twirled for about 30 s and retained for 5 min. Thetreatment was conducted once daily for 2 weeks. The duration of exhaustion training was recorded. Serum lactic acid (LA), lactate dehydrogenase (LDH) and creatine kinase (CK) contents were detected by LA oxidase method, immune-suppressive assay and enzyme-linked immunoassay, respectively.
  
  
  RESULTS
  Compared with the training-fatigue (model) group, the duration of the exhausted swimming of the acupuncture group was obviously longer (P < 0.05). In comparison with the control group, serum LA, LDH and CK contents were increased considerably in the model and acupuncture groups (P < 0.05, P < 0.01), while compared with the model group, serum LDH level of acupuncture group was increased significantly (P < 0.05), and serum CK level of acupuncture group decreased obviously (P < 0.05). No significant difference was found between the model and acupuncture groups in serum LA content (P > 0.05).
  
  
  CONCLUSIONS
  Acupuncture can effectively improve the motor ability of the training-fatigue mice, which may be closely relevant to its effects in upregulating LDH activity and reducing serum CK.
authors.affiliation: College of Acu-moxibustion and Massage, Nanjing University of Chinese Medicine, Nanjing 210046, China. gyh6411@yahoo.com.cn
authors.forename: Yi-Huang
authors.initials: YH
authors.lastname: Gu
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Ren
authors.forename: Yun-Chuan
authors.initials: YC
authors.lastname: Wu
authors.forename: Shou-Dong
authors.initials: SD
authors.lastname: Li
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Shi
mesh_terms: D015670:Acupuncture Therapy; D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D005221:Fatigue / Q000097:blood / Q000503:physiopathology* / Q000628:therapy*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008297:Male; D051379:Mice; D009048:Motor Skills; D018763:Muscle Fatigue; D011897:Random Allocation; D013550:Swimming
chemical_list: D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase