title: Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells.
abstract: To investigate the role of ROS in the helicobacter pylori (Hp) induced mtDNA mutations, AGS cells were treated by extracts of Hp11638 or Hp11638M. The ROS levels, cytochrome C reductions, and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels, cytochrome C reduction and mtDNA mutations were markedly increased and cell viability decreased after treatment with both Hp extracts, and 616 mutations were detected in D-Loop region and 3 heteroplasmic point mutations in the Cytb gene. No mutations were found in the coding region. The mutation rates of mtDNA D-Loop region were positively correlated with the ROS levels and negatively to the ATP levels.
authors.affiliation: Department of Clinical Laboratory, Huadong Sanatorium, Wuxi, Jiangsu Province 214065, China. dochuang@live.cn
authors.forename: Xue-Wen
authors.initials: XW
authors.lastname: Huang
authors.forename: Rui-Hua
authors.initials: RH
authors.lastname: Luo
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Zhong-Ze
authors.initials: ZZ
authors.lastname: Shen
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Huang
authors.forename: Xian-Yuan
authors.initials: XY
authors.lastname: An
authors.forename: Lan-Jing
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Yu-Zheng
authors.initials: YZ
authors.lastname: Huang
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D001483:Base Sequence; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D045744:Cell Line, Tumor; D045303:Cytochromes b / Q000235:genetics; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000235:genetics*; D016480:Helicobacter pylori / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D002457:Cell Extracts; D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; D045303:Cytochromes b