title: Two new steroidal saponins, hylodoside A and novaeguinoside Y, from the starfish Leptasterias hylodes reticulata and Culcita novaeguineae (juvenile).
abstract: New steroid glycosides, hylodoside A (1) and novaeguinoside Y (2), along with previously known polyhydroxylated steroids (3-7) were isolated from the ethanolic extracts of the starfish Leptasterias hylodes reticulata and Culcita novaeguineae (juvenile). The structures have been elucidated using 1D and 2D NMR spectra (1H, 13C, DEPT, COSY-45, 1D-TOCSY, HSQCGP, ROESY, HSQC and HMBCGP) and mass spectrometry. Compound 3 was shown to inhibit the growth of Staphylococcus aureus up to 10% from the control at a concentration of 1 mg/mL. Steroids 1, 2 and 5 showed moderate hemolytic activity in the mouse erythrocytes assay. Compounds 3, 4 and 6 displayed pH-depended hemolytic properties.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Vladivostok, Prospect 100-letya Vladivostoka, 159, Russian Federation. levina@piboc.dvo.ru
authors.forename: Eleonora V
authors.initials: EV
authors.lastname: Levina
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Kalinovsky
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Dmitrenok
authors.forename: Ekaterina A
authors.initials: EA
authors.lastname: Martyyas
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Stonik
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D053878:Hemolytic Agents / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry* / Q000494:pharmacology; D013215:Starfish / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D053878:Hemolytic Agents; D012503:Saponins; C555565:hylodoside A; C555564:novaeguinoside Y