title: PrP 106-126 altered PrP mRNA gene expression in mouse microglia BV-2 cells.
abstract: Prion diseases are infectious and fatal neurodegenerative diseases. The pathogenic agent is an abnormal prion protein aggregate. Microglial activation in the centre nervous system is a characteristic feature of prion disease. In this study, we examined the effect of PrP 106-126 on PrP mRNA gene expression in Mouse microglia cells BV-2 by real-time quantitative PCR. PrP mRNA expression level was found to be significantly increased after 18 h exposure of BV-2 cells to PrP 106-126, with 3-fold increase after 18 h and 4.5-fold increase after 24 h and BV-2 cells proliferating occurred correspondingly. Our results provide the first in vitro evidence of the increase of PrP mRNA levels in microglial cells exposed to PrP 106-126, and indicate that microglial cells might play a critical role in prion pathogenesis.
authors.affiliation: The state key Lab of Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.forename: Yu-rong
authors.initials: YR
authors.lastname: Li
authors.forename: Gui-hua
authors.initials: GH
authors.lastname: Wang
authors.forename: Xiang-mei
authors.initials: XM
authors.lastname: Zhou
authors.forename: De-ming
authors.initials: DM
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015870:Gene Expression; D051379:Mice; D017628:Microglia; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011328:Prions / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D011328:Prions; D012333:RNA, Messenger; C082632:prion protein (106-126)