title: Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94.
abstract: PpAzoR, an FMN-dependent NADPH azoreductase from Pseudomonas putida MET94, has been crystallized using the sitting-drop vapour-diffusion technique. The crystals diffracted to 1.6 Å resolution using synchrotron radiation and belonged to the orthorhombic space group F222, with unit-cell parameters a=72.1, b=95.5, c=146.1 Å. Data sets were collected from the native protein to 2.2 Å resolution using in-house equipment and to 1.6 Å resolution using synchrotron radiation and the three-dimensional structure was determined by the molecular-replacement method.
authors.affiliation: Instituto de Tecnologia Quimica e Biológica, Universidade Nova de Lisboa, Avenida da República, 2780-257 Oeiras, Portugal.
authors.forename: Bruno
authors.initials: B
authors.lastname: Correia
authors.forename: Zhenjia
authors.initials: Z
authors.lastname: Chen
authors.forename: Sónia
authors.initials: S
authors.lastname: Mendes
authors.forename: Lígia O
authors.initials: LO
authors.lastname: Martins
authors.forename: Isabel
authors.initials: I
authors.lastname: Bento
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry*; D009601:Nitroreductases; D011487:Protein Conformation; D016958:Pseudomonas putida / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase