title: [Study on protective effect of monosialoganglioside (GM1) on injury induced by oxygen glucose deprivation/reperfusion in rat hippocampal slices].
abstract: OBJECTIVE
  To investigate the protective effect of monosialoganglioside (GM1) on injury induced by oxygen glucose deprivation/reperfusion (OGD/Rep) in rat hippocampal slices.
  
  
  METHODS
  The protective effects of GM1 on hippocampal slices after OGD/Rep were observed by detecting the light transmittance (LT) changes of rat hippocampal slices and 2, 3, 5-triphenyltetrazolium chloride (TTC) staining of rat hippocampal slices.
  
  
  RESULTS
  (1) In four groups treated with 0 (control), 0.1, 1.0, 10 micromol/L GM1, the peak of light transmittance (LT) in the slices treated with 1.0 micromol/L GM1 was significantly lower than that of the control and the group treated with 0.10 micromol/L GM1 (P < 0.01, ANOVA), while the peak of LT in the slices treated with 10.0 micromol/L GM1 was significantly lower than that of the other groups (P < 0.01, ANOVA). The time to reach the peak of LT in four groups was significantly different from each other (P < 0.05, Kruskal-Wallis test). The time to reach the peak of LT in the group treated with 1 micromol/L GM1 was the significantly longer than that in the control (P < 0.01, Mann-Whitney U test). (2) There was characteristic dose-response relationship between GM1 and TTC staining of rat hippocampal slices. In the five groups, treated with 0 (control), 0.01, 0.1, 1.0, 10 micromol/L GM1 respectively, TTC staining in the group treated with 1 micromol/L GM1 was the deepest (P < 0.05 vs. control, 0.01 and 0.1 micromol/L GM1 group, ANOVA), and the next was in the group treated with 10 micromol/L GM1 (P < 0.05 vs. control and 0.01 micromol/L GM1 group, ANOVA).
  
  
  CONCLUSIONS
  GM1 could protect injury induced by OGD/Rep in rat hippocampal slices effectively in vitro.
authors.affiliation: Department of Neurology, 2nd Affiliated Hospital, College of Medical Sciences, Zhejiang Univercity, Hangzhou 310009, China.
authors.forename: Jian-ren
authors.initials: JR
authors.lastname: Liu
authors.forename: Shui-jiang
authors.initials: SJ
authors.lastname: Song
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: Wei
authors.forename: Men-ling
authors.initials: ML
authors.lastname: Wang
authors.forename: Qiu-fu
authors.initials: QF
authors.lastname: Ge
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ruo-ying
authors.initials: RY
authors.lastname: Liu
mesh_terms: D000818:Animals; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency; D006624:Hippocampus / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010100:Oxygen; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury
chemical_list: D005677:G(M1) Ganglioside; D005947:Glucose; D010100:Oxygen