title: Multi-spectral imaging analysis of HSPB1 expression in head and neck squamous cell carcinoma.
abstract: The objective of this study was to obtain more accurate quantitation of HSPB1 expression in HNSCC using a novel quantitative protein expression analysis system based on multispectral imaging. The study was a retrospective laboratory study of HNSCC patients treated at tertiary care academic medical center. Archival tissue samples from forty seven patients with HNSCC were subjected to immunohistochemistry using primary antibody to HSPB1. Seven of the patients had early stage cancers (TNM stage I/II) and forty patients had advanced stage cancers (TNM stage III/IV). HSPB1 expression was increased in advanced stage versus early stage cancers. Further investigation of HSPB1 as a potential biomarker for HNSCC is warranted.
authors.affiliation: Department of Otolaryngology/Head and Neck Surgery, Medical College of Georgia, Augusta, GA 30912, USA.
authors.forename: Julien A
authors.initials: JA
authors.lastname: Norton
authors.forename: Paul M
authors.initials: PM
authors.lastname: Weinberger
authors.forename: Mark A
authors.initials: MA
authors.lastname: Merkley
authors.forename: Sunny S
authors.initials: SS
authors.lastname: Khichi
authors.forename: Lana L
authors.initials: LL
authors.lastname: Jackson
authors.forename: William S
authors.initials: WS
authors.lastname: Dynan
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016009:Chi-Square Distribution; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006360:Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018832:Molecular Chaperones; D009367:Neoplasm Staging; D012189:Retrospective Studies; D013057:Spectrum Analysis / Q000295:instrumentation*; D018709:Statistics, Nonparametric
chemical_list: D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones