title: An observational assessment method for aging laboratory rats.
abstract: The rapid growth of the aging human population highlights the need for laboratory animal models to study the basic biologic processes of aging and susceptibility to disease, drugs, and environmental pollutants. Methods are needed to evaluate the health of aging animals over time, particularly methods for efficiently monitoring large research colonies. Here we describe an observational assessment method that scores appearance, posture, mobility, and muscle tone on a 5-point scale that can be completed in about 1 min. A score of 1 indicates no deterioration, whereas a score of 5 indicates severe deterioration. Tests were applied to male Brown Norway rats between 12 and 36 mo of age (n = 32). The rats were participating concurrently in experiments on the behavioral effects of intermittent exposure (approximately every 4 mo) to short-acting environmental chemicals. Results demonstrated that aging-related signs of deterioration did not appear before 18 mo of age. Assessment scores and variability then increased with age. Body weights increased until approximately 24 mo, then remained stable, but decreased after 31 mo for the few remaining rats. The incidence of death increased slightly from 20 to 28 mo of age and then rose sharply; median survival age was approximately 30 mo, with a maximum of 36 mo. The results indicate that our observational assessment method supports efficient monitoring of the health of aging rats and may be useful in studies on susceptibility to diseases, drugs, and toxicants during old age.
authors.affiliation: Neurotoxicology Branch, Toxicity Assessment Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, North Carolina, USA.
authors.forename: Pamela M
authors.initials: PM
authors.lastname: Phillips
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Jarema
authors.forename: David M
authors.initials: DM
authors.lastname: Kurtz
authors.forename: Robert C
authors.initials: RC
authors.lastname: MacPhail
mesh_terms: D000375:Aging; D000818:Animals; D000830:Animals, Laboratory / Q000502:physiology*; D008297:Male; D019370:Observation / Q000379:methods*; D051381:Rats; D011914:Rats, Inbred BN