title: Molecular characterization and revised systematics of Microdiaphanosoma arcuatum (Ciliophora, Colpodea).
abstract: Microdiaphanosoma arcuatum Wenzel, 1953 is a world-wide distributed ciliate, recorded mainly in soil samples, which we have also identified in ground water samples from South Africa. This ciliate has been frequently overlooked or not determined due to its small size, ∼12 μm. The genus Microdiaphanosoma is nowadays included in the class Colpodea, order Bryometopida, family Kreyellidae. The first complete small subunit (SSU) rDNA gene sequence of this ciliate was obtained from a South African isolate. Phylogenetic analysis including available SSU rDNA sequences from another Colpodea species in the GenBank strongly supported the position of M. arcuatum within the order Cyrtolophosidida instead of the order Bryometopida. The analysis also suggested a sister relationship between this species and species from the family Cyrtolophosididae.
authors.affiliation: Department of General Ecology, Cologne Biocenter, University of Cologne, Cologne, Germany. pablo.quintela@unikoeln.de
authors.forename: Pablo
authors.initials: P
authors.lastname: Quintela-Alonso
authors.forename: Frank
authors.initials: F
authors.lastname: Nitsche
authors.forename: Hartmut
authors.initials: H
authors.lastname: Arndt
mesh_terms: D016798:Ciliophora / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005618:Fresh Water / Q000469:parasitology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D013019:South Africa
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal