title: Enhanced intracellular Ca2+ concentrations in Escherichia coli and Bacillus subtilis after addition of oligosaccharide elicitors.
abstract: Elicitation can lead to overproduction of secondary metabolites in plants and microbes. Potential changes in cytosolic Ca(2+) levels in bacteria were studied in response to elicitation. We report, for the first time, the effect of oligosaccharide elicitors on intracellular Ca(2+) levels. The apoaequorin gene was cloned into Escherichia coli DH5α and Bacillus subtilis 1604 cultures. Addition of elicitors, oligoguluronate and mannan oligosaccharides, to the cultures caused up to 11-fold increase in cytosolic Ca(2+) in E. coli and tenfold increase in B. subtilis. These increases in Ca(2+) levels could therefore contribute to the enhancement of secondary metabolite levels.
authors.affiliation: Applied Biotechnology Research Group, Department of Molecular & Applied Biosciences, University of Westminster, 115 New Cavendish Street, London SW1 6UW, UK.
authors.forename: Tania M
authors.initials: TM
authors.lastname: Murphy
authors.forename: Alex Y
authors.initials: AY
authors.lastname: Nilsson
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
authors.forename: Anthony
authors.initials: A
authors.lastname: Harrop
authors.forename: Keith
authors.initials: K
authors.lastname: Dixon
authors.forename: Tajalli
authors.initials: T
authors.lastname: Keshavarz
mesh_terms: D000331:Aequorin / Q000235:genetics / Q000378:metabolism; D001059:Apoproteins / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000737:chemistry* / Q000378:metabolism*; D002118:Calcium / Q000032:analysis*; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001059:Apoproteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; C060014:apoaequorin; D000331:Aequorin; D002118:Calcium