title: Evaluation of immunohistochemistry for the diagnosis of sporotrichosis in dogs.
abstract: The aim of this study was to apply immunohistochemistry (IHC) for the diagnosis of canine sporotrichosis and to compare this method with the Grocott's silver stain (GSS) and periodic acid Schiff (PAS) techniques. Eighty-seven dogs with sporotrichosis (group 1) and 35 with American tegumentary leishmaniosis (ATL) (group 2) were studied. The fungus was detected in group 1 by GSS, PAS and IHC. IHC was also applied to group 2 to evaluate the occurrence of cross-reactions. PAS, GSS and IHC detected yeast cells in 19.5%, 43.7% and 65.5% of the group 1 cases, respectively. The detection of intracellular antigens of Sporothrix schenckii by IHC increased the sensitivity of the histological diagnosis to 80.5%. No positive reaction was observed in ATL lesions. The results suggest that IHC may be indicated for the diagnosis of sporotrichosis because of its higher diagnostic sensitivity.
authors.affiliation: Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO), Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz (IPEC, FIOCRUZ), Av. Brasil, 436, Manguinhos, 21040-360 Rio de Janeiro, RJ, Brazil. luisahmiranda@gmail.com
authors.forename: Luisa H M
authors.initials: LH
authors.lastname: Miranda
authors.forename: Leonardo P
authors.initials: LP
authors.lastname: Quintella
authors.forename: Rodrigo C
authors.initials: RC
authors.lastname: Menezes
authors.forename: Isabele B
authors.initials: IB
authors.lastname: dos Santos
authors.forename: Raquel V C
authors.initials: RV
authors.lastname: Oliveira
authors.forename: Fabiano B
authors.initials: FB
authors.lastname: Figueiredo
authors.forename: Leila M
authors.initials: LM
authors.lastname: Lopes-Bezerra
authors.forename: Tânia M P
authors.initials: TM
authors.lastname: Schubach
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D003429:Cross Reactions; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D016773:Leishmaniasis, Cutaneous / Q000662:veterinary; D010503:Periodic Acid-Schiff Reaction / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000379:methods / Q000662:veterinary; D012867:Skin / Q000382:microbiology; D013175:Sporothrix / Q000276:immunology; D013174:Sporotrichosis / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000942:Antigens, Bacterial