title: [Effects of butylphthalide on the apoptosis of PC-12 cells under the induction of β-amyloid peptide].
abstract: OBJECTIVE
  To study the protective effects of butylphthalide (NBP) on the Aβ(25-35)-induced apoptosis in PC-12 cells.
  
  
  METHODS
  The apoptosis of PC-12 cell was analyzed by MTT assay, transmission electron microscope and PI method at different concentrations of NBP (0.1, 1.0, 10, 100 µmol/L) with the addition of Aβ or not. The expressions of Bcl-2 and cytochrome C (Cyt-C) were detected by Western blot.
  
  
  RESULTS
  As demonstrated by the MTT assay, the values of cell viability were 76.5% ± 1.1%, 84.2% ± 1.3%, 89.5% ± 1.3% and 81.9% ± 1.9% at various concentration (0.1, 1.0, 10, 100 µmol/L) of NBP respectively. The model group was 71.7% ± 1.4%. It was revealed that the former could significantly prevent the cell viability under the induction of Aβ(25-35) (P < 0.05). A pretreatment with 10 µmol/L NBP could significantly inhibit the decrease of viability under the induction of Aβ(25-35) (P < 0.05). PI showed that the apoptosis rate of the 10 µmol/L NBP treatment group was significantly lower than that of the model group. Under electron microscope, the characteristics of cell apoptosis were significant in the model group. And the cell morphology of the 10 µmol/L NBP treatment group was normal. The expression rate of Bcl-2 protein in the 10 µmol/L NBP treatment group was obviously higher than that in the model group. Cyt-C was weakly expressed in nerve cells of the normal and the 10 µmol/L NBP groups. But it had a strong expression in the model group.
  
  
  CONCLUSIONS
  NBP prevents the injury of PC12 cells by Aβ. And the mechanism may be related to the elevated level of Bcl-2 and the inhibited mitochondrial release of Cyt-C.
authors.affiliation: Department of Neurology, China Medical University, Shenyang 110001, China.
authors.forename: Yu-huan
authors.initials: YH
authors.lastname: Cui
authors.forename: Chao-dong
authors.initials: CD
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Yu-lei
authors.initials: YL
authors.lastname: Wei
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001572:Benzofurans / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats
chemical_list: D016229:Amyloid beta-Peptides; D001572:Benzofurans; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C027125:3-n-butylphthalide