title: Performance tests of a novel coaxial tube catheter in an in vitro model of intracranial cell delivery.
abstract: We have tested prototypes of a novel coaxial tube catheter in an in vitro gel model of cell delivery into the brain. Devices 1.6 and 2.0 mm outer diameter were used to deliver PC 12 cells (concentration = 10⁶ cells ml⁻¹ at 1 μl min⁻¹ into a 5 ml sandwich of collagen and 0.1% agarose, with and without follow-on infusions of nerve growth factor (NGF). Post-infusion microscopic imaging (40X) at the infusion sites was then carried out over 7-day periods. The results showed that under these experimental conditions, it was possible to use these catheters to deliver cells without either leakage of trapped air into the gel or reflux of the cell suspension along the catheter insertion track. Differentiation of the NGF-treated cells was observed.
authors.affiliation: Department of Neurosurgery, Virginia Commonwealth University, PO Box 980631, Richmond, Virginia 23298-0631, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Panse
authors.forename: H L
authors.initials: HL
authors.lastname: Fillmore
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Chen
authors.forename: G T
authors.initials: GT
authors.lastname: Gillies
authors.forename: W C
authors.initials: WC
authors.lastname: Broaddus
mesh_terms: D000818:Animals; D002404:Catheterization; D002454:Cell Differentiation / Q000187:drug effects; D064987:Cell- and Tissue-Based Therapy / Q000295:instrumentation; D020932:Nerve Growth Factor / Q000008:administration & dosage / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats
chemical_list: D020932:Nerve Growth Factor