title: A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives.
abstract: The industrial Leuconostoc strain B/110-1-2 producing dextran and dextran derivatives was taxonomically identified by 16S rRNA as L. citreum. Its dextransucrase enzymes were characterized according to their cellular location and reaction specificity. In the presence of sucrose, the strain B/110-1-2 produced two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46%) at 160-180 kDa. Two open reading frames (ORF) coding for L. citreum strain B/110-1-2 dextransucrases were identified. One of them shared a 52% identity with the alternansucrase ASR of L. citreum NRRL B-1355 and with a putative annotated alternansucrase sequence found in the genome of L. citreum KM20. The structural analysis (HPAEC-PAD, HPSEC, and (13)C-NMR) of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases suggest this novel glucansucrase has specificity similar to a dextransucrase but not to an alternansucrase, producing a soluble linear dextran with glucose molecules linked mainly in α-1,6 and α-1,3 with α-1,4 branches. These results enhance the understanding of this industrially significant strain and will aid in distinguishing between physiologically similar Leuconostoc spp. strains.
authors.affiliation: Cuban Research Institute on Sugarcane By-Products (ICIDCA), PO Box 4026 CH-10, 11000, Havana, Cuba. reynaldo.fraga@icidca.edu.cu
authors.forename: Reinaldo Fraga
authors.initials: RF
authors.lastname: Vidal
authors.forename: Aidín
authors.initials: A
authors.lastname: Martínez
authors.forename: Claire
authors.initials: C
authors.lastname: Moulis
authors.forename: Pierre
authors.initials: P
authors.lastname: Escalier
authors.forename: Sandrine
authors.initials: S
authors.lastname: Morel
authors.forename: Magali
authors.initials: M
authors.lastname: Remaud-Simeon
authors.forename: Pierre
authors.initials: P
authors.lastname: Monsan
mesh_terms: D003911:Dextrans / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D007218:Industrial Microbiology; D007934:Leuconostoc / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis; D013379:Substrate Specificity; D013395:Sucrose / Q000378:metabolism
chemical_list: D003911:Dextrans; D009844:Oligosaccharides; D013395:Sucrose; D016695:Glycosyltransferases; D005964:Glucosyltransferases; C033837:alternansucrase; C020025:dextransucrase