title: Inactivation of Salmonella in liquid egg albumen by antimicrobial bottle coatings infused with allyl isothiocyanate, nisin and zinc oxide nanoparticles.
abstract: OBJECTIVE
  To develop an antimicrobial bottle coating effective at inhibiting the growth of Salmonella in liquid egg albumen (egg white) and reduce the risk of human Salmonellosis.
  
  
  RESULTS
  Four-ounce glass jars were coated with a mixture of polylactic acid (PLA) polymer and antimicrobial compounds containing 100-500 μl allyl isothiocyanate (AIT), 250 mg nisin, 250 mg zinc oxide nanoparticles per jar or their combinations. The coated jars contained 100 ml of liquid egg white (LEW) inoculated with a three-strain Salmonella enterica ssp. enterica cocktail at populations of 10(3) or 10(7) CFU ml(-1) and stored at 10°C for 28 days. The PLA coating with 500 μl AIT completely inactivated 3 and 7 log CFU ml(-1) of Salmonella after 7 and 21 days of storage, respectively. The PLA coating with 200 μl AIT in combination with 250 mg nisin reduced Salmonella populations to an undetectable level (<10 CFU ml(-1) ) after 21 days of storage.
  
  
  CONCLUSIONS
  PLA coatings containing AIT alone or in combination with nisin effectively inactivated salmonellae in LEW.
  
  
  CONCLUSIONS
  This study demonstrated the commercial potential of applying the antimicrobial bottle coating method to liquid eggs and possibly other fluid food products.
authors.affiliation: US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. tony.jin@ars.usda.gov
authors.forename: T
authors.initials: T
authors.lastname: Jin
authors.forename: J B
authors.initials: JB
authors.lastname: Gurtler
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D004529:Egg White / Q000382:microbiology*; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D053758:Nanoparticles; D009561:Nisin / Q000494:pharmacology*; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development; D012478:Salmonella Food Poisoning / Q000517:prevention & control; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D017879:Isothiocyanates; D009561:Nisin; C004471:allyl isothiocyanate; D015034:Zinc Oxide