title: Hyper production of alkali stable xylanase in lesser duration by Bacillus pumilus SV-85S using wheat bran under solid state fermentation.
abstract: High level production of an extracellular cellulase-poor alkali stable xylanase has been conceded from newly isolated Bacillus pumilus SV-85S under solid state fermentation using wheat bran as a substrate. Optimization of the fermentation conditions enhanced the enzyme production to 73,000 ± 1,000 IU/g dry substrate, which was 13.8-fold higher than unoptimized conditions (5,300 IU/g). The enzyme titre was highest after 48 h of incubation at 30°C with 1:3 ratios of substrate to moistening agent using wheat bran as a carbon source. The enzyme could be produced in significant levels by using either tap water or distilled water alone as a moistening agent. An elevated production of xylanase by B. pumilus SV-85S in the presence of wheat bran, a cheap and easily available agro-residue, in shorter duration would apparently reduce the enzyme cost substantially. The enzyme was completely stable over a broad pH (5-11) range and retained 52% of its activity at a temperature of 70°C for 30 min. The desired characteristics of this enzyme together with economic production would be important for its application in paper and pulp industry.
authors.affiliation: Department of Biochemistry, Kurukshetra University, Kurukshetra 136 119, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Nagar
authors.forename: Anuradha
authors.initials: A
authors.lastname: Mittal
authors.forename: Davender
authors.initials: D
authors.lastname: Kumar
authors.forename: Lalit
authors.initials: L
authors.lastname: Kumar
authors.forename: Ramesh Chander
authors.initials: RC
authors.lastname: Kuhad
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Gupta
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004043:Dietary Fiber / Q000494:pharmacology*; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D014995:Xylosidases / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004043:Dietary Fiber; D014995:Xylosidases