title: [Expression and function analysis of chemokine receptor CCR6 in HEK293 cells].
abstract: OBJECTIVE
  To construct a cell line which stably expresses human chemokine receptor 6 (CCR6).
  
  
  METHODS
  The human CCR6 cDNA and plasmid G were co-transfected into HEK 293 cells and the clones stably expressing CCR6 were picked out. The expression of CCR6 in HEK293 cells was detected by RT-PCR, Western blotting, immunofluorescence test, calcium mobilization and in vitro chemotaxis assay.
  
  
  RESULTS
  The transfected HEK293 cells could stably express functional human CCR6.
  
  
  CONCLUSIONS
  Successfully establish a cell line which stably express human CCR6 and lays the foundation for its biological function's study and specific antagonist screening.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou 730000, China. weiweiw04@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Lan
authors.initials: L
authors.lastname: Gao
authors.forename: Fei-fei
authors.initials: FF
authors.lastname: Zhang
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Cui
authors.forename: Xin
authors.initials: X
authors.lastname: Xie
mesh_terms: D015153:Blotting, Western / Q000379:methods; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D010957:Plasmids / Q000235:genetics; D054399:Receptors, CCR6 / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: C106311:CCR6 protein, human; D018076:DNA, Complementary; D054399:Receptors, CCR6