title: DNA-templated protein arrays for single-molecule imaging.
abstract: Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.
authors.affiliation: Department of Physics, Clarendon Laboratory, University of Oxford, Oxford, UK.
authors.forename: Daniele N
authors.initials: DN
authors.lastname: Selmi
authors.forename: Roslin J
authors.initials: RJ
authors.lastname: Adamson
authors.forename: Helen
authors.initials: H
authors.lastname: Attrill
authors.forename: Alan D
authors.initials: AD
authors.lastname: Goddard
authors.forename: Robert J C
authors.initials: RJ
authors.lastname: Gilbert
authors.forename: Anthony
authors.initials: A
authors.lastname: Watts
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Turberfield
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D019544:Equipment Failure Analysis; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*
chemical_list: D004247:DNA