title: The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs.
abstract: Matrix metalloproteinases (MMPs) bound to dentin contribute to the progressive degradation of collagen fibrils in hybrid layers created by dentin adhesives. This study evaluated the MMP-inhibiting potential of quaternary ammonium methacrylates (QAMs), with soluble rhMMP-9 and a matrix-bound endogenous MMP model. Six different QAMs were initially screened by a rhMMP-9 colorimetric assay. For the matrix-bound endogenous MMPs, we aged demineralized dentin beams for 30 days in calcium- and zinc-containing media (CM; control), chlorhexidine, or QAMs in CM to determine the changes in dry mass loss and solubilization of collagen peptides against baseline levels. The inhibitory effects of QAMs on soluble rhMMP-9 varied between 34 and 100%. Beams incubated in CM showed a 29% decrease in dry mass (p < 0.05), whereas beams incubated with QAMs showed only 0.2%-6% loss of dry mass. Significantly more solubilized collagen was detected from beams incubated in CM (p < 0.05). It is concluded that QAMs exhibited dentin MMP inhibition comparable with that of chlorhexidine, but required higher concentrations.
authors.affiliation: Department of Prosthodontics and Turku Clinical Biomaterials Center, University of Turku, Institute of Dentistry, Lemminkaisenkatu 2, FI-20520 Turku, Finland. arztez@utu.fi
authors.forename: A
authors.initials: A
authors.lastname: Tezvergil-Mutluay
authors.forename: K A
authors.initials: KA
authors.lastname: Agee
authors.forename: T
authors.initials: T
authors.lastname: Uchiyama
authors.forename: S
authors.initials: S
authors.lastname: Imazato
authors.forename: M M
authors.initials: MM
authors.lastname: Mutluay
authors.forename: M
authors.initials: M
authors.lastname: Cadenaro
authors.forename: L
authors.initials: L
authors.lastname: Breschi
authors.forename: Y
authors.initials: Y
authors.lastname: Nishitani
authors.forename: F R
authors.initials: FR
authors.lastname: Tay
authors.forename: D H
authors.initials: DH
authors.lastname: Pashley
mesh_terms: D000293:Adolescent; D000498:Allyl Compounds / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D002794:Choline / Q000031:analogs & derivatives / Q000494:pharmacology; D003094:Collagen / Q000187:drug effects; D003124:Colorimetry; D003804:Dentin / Q000187:drug effects* / Q000201:enzymology; D003808:Dentin Solubility / Q000187:drug effects; D003890:Desiccation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D011994:Recombinant Proteins; D050337:Trimethyl Ammonium Compounds / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000498:Allyl Compounds; D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D050337:Trimethyl Ammonium Compounds; C089392:methacryloyloxydodecylpyridinium bromide; C023876:methacrylylcholine; C508190:diallyldimethylammonium chloride; D003094:Collagen; D002794:Choline; D002710:Chlorhexidine; D006909:Hydroxyproline