title: Proteomic analysis of two types of exosomes in human whole saliva.
abstract: Saliva contains a large number of proteins that participate in the protection of oral tissue. Exosomes are small vesicles (30-100 nm in diameter) with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We have recently demonstrated that exosomes are present in human whole saliva. In this study, we found that whole saliva contained at least two types of exosomes (exosome I and exosome II) that are different in size and protein composition. Proteomic analysis revealed that both types of exosomes contained Alix, Tsg101 and Hsp70, all exosomal markers, immunoglobulin A and polymeric immunoglobulin receptor, whereas they had different protein compositions. Most of dipeptidyl peptidase IV known as CD26 in whole saliva, was present on the exosome II and metabolically active in cleaving chemokines (CXCL11 and CXCL12). Human whole saliva exosomes might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Ichihara, Chiba 290–0193, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Yuri
authors.initials: Y
authors.lastname: Miura
authors.forename: Akira
authors.initials: A
authors.lastname: Harazono
authors.forename: Masami
authors.initials: M
authors.lastname: Kanai-Azuma
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Akimoto
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.forename: Teruhide
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Tosifusa
authors.initials: T
authors.lastname: Toda
authors.forename: Tamao
authors.initials: T
authors.lastname: Endo
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Tsubuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Yanoshita
mesh_terms: D015415:Biomarkers; D055354:Exosomes / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D040901:Proteomics; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D012471:Salivary Proteins and Peptides