title: HPLC/DAD/MS and antioxidant activity of isoflavone-based food supplements.
abstract: Isoflavones are polyphenolic compounds found mainly in legumes the benefits of which have been widely studied and attributed in particular to their phytoestrogenic activity. The aim of this study was to evaluate the quali-quantitative composition of food supplements based on soy isoflavones (Glycine max L.) and red clover (Trifolium pratense). Six commercial food supplements (five soy-based and one red clover-based) were analyzed by HPLC/DAD/MS. Genistein, daidzein, glycitein, biochanin A and formononetin derivatives (glycosides and acylglycosides) were identified in the analyzed samples. Also the antiradical activities (towards the DPPH* radical) and Fe2+ chelating abilities were compared.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Firenze, via U. Schiff 6, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Romani
authors.forename: Pamela
authors.initials: P
authors.lastname: Vignolini
authors.forename: Annalisa
authors.initials: A
authors.lastname: Tanini
authors.forename: Barbara
authors.initials: B
authors.lastname: Pampaloni
authors.forename: Daniela
authors.initials: D
authors.lastname: Heimler
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D007529:Isoflavones / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D013025:Glycine max / Q000737:chemistry; D029921:Trifolium / Q000737:chemistry
chemical_list: D000975:Antioxidants; D007529:Isoflavones