title: Asc1p, a ribosomal protein, plays a pivotal role in cellular adhesion and virulence in Candida albicans.
abstract: Candida albicans, the common human fungal pathogen, can switch morphology from yeast to pseudohyphal or hyphal form upon various environmental cues. It is well-known that the ability of morphological conversion and adhesive growth renders C. albicans virulent. It is noteworthy that every factor involved in the morphogenesis is known to be important for the virulence of this pathogen. To examine a functional relevance of Asc1p, a ribosomal protein, in morphogenesis and virulence, an asc1 homozygous null mutant was generated. Although a normal morphological transition of the asc1 deletion strain in liquid media was found, it did not change its morphology on solid media. Moreover, the adhesion activity and hyphal-specific gene expression were defective due to ASC1 deletion. Finally, it was found that the asc1 null mutant was avirulent in a mouse model. These results strongly suggested that Asc1p a component of the 40S ribosomal subunit and a signal transducer, plays a pivotal role in cellular adhesion and virulence through regulation of specific gene expression in C. albicans.
authors.affiliation: Laboratory of Biochemistry, School of Life Sciences and Biotechnology, and BioInstitute, Korea University, Seoul, 136-701, Republic of Korea.
authors.forename: Se Woong
authors.initials: SW
authors.lastname: Kim
authors.forename: Yoo Jin
authors.initials: YJ
authors.lastname: Joo
authors.forename: Joon
authors.initials: J
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002176:Candida albicans / Q000166:cytology / Q000235:genetics / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology / Q000401:mortality; D002448:Cell Adhesion; D003470:Culture Media / Q000737:chemistry; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D016019:Survival Analysis; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C556216:Asc1 protein, Candida albicans; D003470:Culture Media; D005656:Fungal Proteins; D037521:Virulence Factors