title: Synthesis and DNA photocleavage by a pyridine-linked bis-acridine chromophore in the presence of copper(II): ionic strength effects.
abstract: We report the synthesis of photonuclease 3 consisting of two acridine rings joined by a 2,6-bis(aminomethyl)pyridine copper-binding linker. In reactions containing micromolar concentrations of 3, irradiation at 419 nm produces efficient, copper(II)-dependent cleavage of plasmid DNA in the presence of the high concentrations of salt that exist in the cell nucleus (150 mM NaCl and 260 mM KCl). The DNA interactions of 3 are compared to an analogous bis-acridine (4) containing a more flexible 2,6-bis{[(methoxycarbonylamino)-ethyl]methylaminomethyl}pyridine unit.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, GA 30302-4098, USA. kbgrant@gsu.edu
authors.forename: Kathryn B
authors.initials: KB
authors.lastname: Grant
authors.forename: Carla A
authors.initials: CA
authors.lastname: Terry
authors.forename: Lourdes
authors.initials: L
authors.lastname: Gude
authors.forename: María-José
authors.initials: MJ
authors.lastname: Fernández
authors.forename: Antonio
authors.initials: A
authors.lastname: Lorente
mesh_terms: D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D009994:Osmolar Concentration; D055668:Photochemical Processes; D017319:Photosensitizing Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D012492:Salts / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000166:Acridines; D017319:Photosensitizing Agents; D012492:Salts; C484811:bis-acridine; D003300:Copper; D004247:DNA