title: Biocompatibility and bioactivity enhancement of Ce stabilized ZrO(2) doped HA coatings by controlled porosity change of Al(2) O(3) substrates.
abstract: Al(2) O(3) substrates with controlled porosity were manufactured from nanosized powders obtained by plasma processing. It was observed that when increasing the sintering temperature the overall porosity was decreasing, but the pores got larger. In a second step, Ce stabilized ZrO(2) doped hydroxyapatite coatings were pulsed laser deposited onto the Al(2) O(3) substrates. It was shown that the surface morphology, consisting of aggregates and particulates in micrometric range, was altered by the substrate porosity and interface properties, respectively. TEM studies evidenced that Ce stabilized ZrO(2) doped HA particulates ranged from 10 to 50 nm, strongly depending on the Al(2) O(3) porosity. The coatings consisted of HA nanocrystals embedded in an amorphous matrix quite similar to the bone structure. These findings were congruent with the increased biocompatibility and bioactivity of these layers confirmed by enhanced growing and proliferation of human mesenchymal stem cells.
authors.affiliation: Laser Department, National Institute for Lasers, Plasma, and Radiation Physics, Magurele, Ilfov, Romania.
authors.forename: Felix
authors.initials: F
authors.lastname: Sima
authors.forename: Carmen
authors.initials: C
authors.lastname: Ristoscu
authors.forename: Diana
authors.initials: D
authors.lastname: Caiteanu
authors.forename: Cristian N
authors.initials: CN
authors.lastname: Mihailescu
authors.forename: Nicolaie
authors.initials: N
authors.lastname: Stefan
authors.forename: Ion N
authors.initials: IN
authors.lastname: Mihailescu
authors.forename: Gabriel
authors.initials: G
authors.lastname: Prodan
authors.forename: Victor
authors.initials: V
authors.lastname: Ciupina
authors.forename: Eriks
authors.initials: E
authors.lastname: Palcevskis
authors.forename: Janis
authors.initials: J
authors.lastname: Krastins
authors.forename: Livia E
authors.initials: LE
authors.lastname: Sima
authors.forename: Stefana M
authors.initials: SM
authors.lastname: Petrescu
mesh_terms: D000537:Aluminum Oxide; D049109:Cell Proliferation; D002563:Cerium; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D016062:Porosity; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*; D015040:Zirconium
chemical_list: D020099:Coated Materials, Biocompatible; D002563:Cerium; D017886:Durapatite; D015040:Zirconium; D000537:Aluminum Oxide; C028541:zirconium oxide