title: Salmonella enterica Enteritidis biofilm formation and viability on regular and triclosan-impregnated bench cover materials.
abstract: Contamination of food contact surfaces by microbes such as Salmonella is directly associated with substantial industry costs and severe foodborne disease outbreaks. Several approaches have been developed to control microbial attachment; one approach is the development of food contact materials incorporating antimicrobial compounds. In the present study, Salmonella enterica Enteritidis adhesion and biofilm formation on regular and triclosan-impregnated kitchen bench stones (silestones) were assessed, as was cellular viability within biofilms. Enumeration of adhered cells on granite, marble, stainless steel, and silestones revealed that all materials were prone to bacterial colonization (4 to 5 log CFU/cm(2)), and no significant effect of triclosan was found. Conversely, results concerning biofilm formation highlighted a possible bacteriostatic activity of triclosan; smaller amounts of Salmonella Enteritidis biofilms were formed on impregnated silestones, and significantly lower numbers of viable cells (1 × 10(5) to 1 × 10(6) CFU/cm(2)) were found in these biofilms than in those on the other materials (1 × 10(7) CFU/cm(2)). All surfaces tested failed to promote food safety, and careful utilization with appropriate sanitation of these surfaces is critical in food processing environments. Nevertheless, because of its bacteriostatic activity, triclosan incorporated into silestones confers some advantage for controlling microbial contamination.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Diana
authors.initials: D
authors.lastname: Rodrigues
authors.forename: Pilar
authors.initials: P
authors.lastname: Teixeira
authors.forename: Rosário
authors.initials: R
authors.lastname: Oliveira
authors.forename: Joana
authors.initials: J
authors.lastname: Azeredo
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004783:Environmental Microbiology; D004866:Equipment Contamination / Q000517:prevention & control; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D006801:Humans; D012477:Salmonella enteritidis / Q000187:drug effects / Q000502:physiology*; D014260:Triclosan / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D014260:Triclosan