title: Alterations of NO synthase isoforms in brain and kidney of rats with genetic and salt hypertension.
abstract: Both brain and peripheral nitric oxide (NO) play a role in the control of blood pressure and circulatory homeostasis. Central NO production seems to counteract angiotensin II-induced enhancement of sympathetic tone. The aim of our study was to evaluate NO synthase (NOS) activity and protein expression of its three isoforms--neuronal (nNOS), endothelial NOS (eNOS) and inducible (iNOS)--in two brain regions involved in blood pressure control (diencephalon and brainstem) as well as in the kidney of young adult rats with either genetic (12-week-old SHR) or salt-induced hypertension (8-week-old Dahl rats). We have demonstrated reduced nNOS and iNOS expression in brainstem of both hypertensive models. In SHR this abnormality was accompanied by attenuated NOS activity and was corrected by chronic captopril treatment which prevented the development of genetic hypertension. In salt hypertensive Dahl rats nNOS and iNOS expression was also decreased in the diencephalon where neural structures important for salt hypertension development are located. As far as peripheral NOS activity and expression is concerned, renal eNOS expression was considerably reduced in both genetic and salt-induced hypertension. In conclusions, we disclosed similar changes of NO system in the brainstem (but not in the diencephalon) of rats with genetic and salt-induced hypertension. Decreased nNOS expression was associated with increased blood pressure due to enhanced sympathetic tone.
authors.affiliation: Cardiovascular Research Center,Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. silvie.hojna@post.cz
authors.forename: S
authors.initials: S
authors.lastname: Hojná
authors.forename: J
authors.initials: J
authors.lastname: Kuneš
authors.forename: J
authors.initials: J
authors.lastname: Zicha
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D006973:Hypertension / Q000201:enzymology* / Q000209:etiology / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D020303:Rats, Inbred Dahl; D017673:Sodium Chloride, Dietary / Q000633:toxicity
chemical_list: D007527:Isoenzymes; D017673:Sodium Chloride, Dietary; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase