title: MiRNA profile in esophageal squamous cell carcinoma: downregulation of miR-143 and miR-145.
abstract: OBJECTIVE
  To investigate the expression profile of miRNA in esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray. The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction (PCR) using TaqMan assay. The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids.
  
  
  RESULTS
  A set of miRNAs was found to be deregulated in the ESCC tissues, and the expression levels of miR-143 and -145 were significantly decreased in most of the ESCC tissues examined. Both miR-143 and miR-145 expression correlated with tumor invasion depth. The transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility, however, the protein level of the previously reported target of miR-145, FSCN1, did not show any significant downregulation.
  
  
  CONCLUSIONS
  These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC. Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, Guangdong Province, China.
authors.forename: Bing-Li
authors.initials: BL
authors.lastname: Wu
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.forename: Ze-Peng
authors.initials: ZP
authors.lastname: Du
authors.forename: Lian-Di
authors.initials: LD
authors.lastname: Liao
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Huang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Fang
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D014162:Transfection
chemical_list: D002352:Carrier Proteins; C502658:FSCN1 protein, human; C542359:MIRN143 microRNA, human; C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins