title: Changes in dietary folate intake differentially affect oxidised lipid and mitochondrial DNA damage in various brain regions of rats in the absence/presence of intracerebroventricularly injected amyloid β-peptide challenge.
abstract: Accumulating evidence suggests that changes in dietary folate intake may modulate the risks of Alzheimer's disease (AD) through as yet unknown mechanisms. The aims of the present study were to investigate how dietary folate affects the brain folate distribution, levels of oxidised lipid and DNA damage in the absence/presence of β-amyloid(25-35) (Aβ) peptide challenge, a pathogenic hallmark of AD. Male Wistar rats were assigned to diets with folic acid at 0 (folate deprivation; FD), 8 (moderate folate; MF) and 8 mg folic acid/kg diet+0·003 % in drinking-water (folate supplementation; FS) for 4 weeks. A single injection of Aβ peptide (1 mg/ml) or the vehicle solution was intracerebroventricularly (icv) administrated to rats a week before killing. Brain folate, a marker of oxidative injury, and neuronal death were assayed. In the absence of an Aβ injection, FD rats showed reduced folate levels, and increased 2-thiobarbituric acid-reactive substances and a mitochondrial (mt)DNA 4834 bp large deletion (mtDNA4834 deletion) in the hippocampus compared with the counterpart brains of control rats (P < 0·05). A single icv injection of Aβ peptide potentiated lipid peroxidation in the medulla of FD rats, which was ameliorated by feeding FD rats with the MF and FS diets (P < 0·05). Feeding the FS diet to Aβ-injected rats enriched brain folate levels and reduced mtDNA4834 deletion in the hippocampal and medullary regions compared with corresponding tissues of Aβ+FD rats (P < 0·05). Aβ+FS rats had reduced rates of neuronal death in the frontal cortex compared with Aβ+FD rats (P < 0·05). Taken together, our data revealed that folate deprivation differentially depleted brain folate levels, and increased lipid peroxidation and mtDNA4834 deletions, particularly, in the hippocampus. Upon Aβ challenge, the FS diet may protect various brain regions against lipid peroxidation, mitochondrial genotoxicity and neural death associated with folate deprivation.
authors.affiliation: Department of Neurology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Ta-Fu
authors.initials: TF
authors.lastname: Chen
authors.forename: Ming-Jang
authors.initials: MJ
authors.lastname: Chiu
authors.forename: Chou-Tz
authors.initials: CT
authors.lastname: Huang
authors.forename: Ming-Chi
authors.initials: MC
authors.lastname: Tang
authors.forename: Sue-Jane
authors.initials: SJ
authors.lastname: Wang
authors.forename: Chia-Chuan
authors.initials: CC
authors.lastname: Wang
authors.forename: Rwei-Fen Syu
authors.initials: RF
authors.lastname: Huang
mesh_terms: D016229:Amyloid beta-Peptides / Q000008:administration & dosage*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001921:Brain / Q000166:cytology*; D016923:Cell Death / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000187:drug effects*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005492:Folic Acid / Q000494:pharmacology*; D057967:Infusions, Intraventricular; D015227:Lipid Peroxidation; D008297:Male; D009474:Neurons / Q000187:drug effects*; D051381:Rats
chemical_list: D016229:Amyloid beta-Peptides; D004272:DNA, Mitochondrial; D005492:Folic Acid