title: Genetic elements in the VP region of porcine parvovirus are critical to replication efficiency in cell culture.
abstract: Factors controlling porcine parvovirus (PPV) replication efficiency are poorly characterized. Two prototype strains of PPV, NADL-2 and Kresse, differ greatly in pathogenic capacity both in vivo and in vitro, yet their genomic sequence is nearly identical (13 single-nucleotide substitutions and a 127-nucleotide noncoding repeated sequence). We have created a series of chimeras of these strains to identify the genetic elements involved in replication efficiency in the host porcine cell line. While the capsid proteins ultimately determine viral replication fitness, interaction between the NS1 protein and the VP gene occurs and involves interaction with the noncoding repeated sequence.
authors.affiliation: INRS-Institut Armand-Frappier, Université du Québec, 531, boul. des Prairies, Laval, Québec, Canada H7V 1B7.
authors.forename: Sandra
authors.initials: S
authors.lastname: Fernandes
authors.forename: Maude
authors.initials: M
authors.lastname: Boisvert
authors.forename: Peter
authors.initials: P
authors.lastname: Tijssen
mesh_terms: D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D028323:Parvovirus, Porcine / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins