title: [Expression of MnSOD mRNA and protein in esophageal squamous cell carcinoma and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression of manganese superoxide dismutase (MnSOD) and to determine the relationship between MnSOD expression and clinicopathological features, biological behaviors in esophageal carcinoma.
  
  
  METHODS
  Immunohistochemistry (SP) and RT-PCR were respectively used to detect the expression of MnSOD in 45 specimens of esophageal carcinoma tissues and normal esophageal mucosa (5 cm distant from the margin of cancer).
  
  
  RESULTS
  The positive rate of MnSOD protein expression was 31.1% in esophageal carcinoma tissues, significantly lower than 86.7% in the normal tissues (P < 0.05). The expressions of MnSOD mRNA and protein were significantly correlated with the lesion length, depths of invasion and histological grade (P < 0.05), but not with lymph node metastasis, lesion site and gross type of the tumor (P > 0.05). The relative content of MnSOD mRNA was (0.310 ± 0.036) and (0.482 ± 0.053) in the cancer and normal tissues, respectively, with a significant difference between the two groups (P < 0.05). The relative content of MnSOD mRNA was significantly related to lesion length, depths of invasion and histological grade (P < 0.05), but not correlated with lymph node status, lesion site and gross type of the tumor (P > 0.05).
  
  
  CONCLUSIONS
  The expression of MnSOD protein and mRNA is decreased in esophageal carcinoma, suggesting that MnSOD gene may be closely associated with the carcinogenesis and the degree of malignancy. Detection of MnSOD expression may be useful in diagnosis, treatment and prognosis of esophageal carcinoma.
authors.affiliation: Department of Radiotherapy, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
authors.forename: Guo-gui
authors.initials: GG
authors.lastname: Sun
authors.forename: Ya-di
authors.initials: YD
authors.lastname: Wang
authors.forename: Xiu-rong
authors.initials: XR
authors.lastname: Yu
authors.forename: Yun-jie
authors.initials: YJ
authors.lastname: Cheng
authors.forename: Shuo
authors.initials: S
authors.lastname: Bai
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiang-ran
authors.initials: XR
authors.lastname: Yang
authors.forename: Xin
authors.initials: X
authors.lastname: Wan
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D013482:Superoxide Dismutase