title: Effect of Fe2+ and Fe3+ ions on human plasma cholinesterase activity.
abstract: OBJECTIVE
  Metals such as iron are needed for physiological functions of the body. However, their excess may cause serious damage including poisoning. The effects of Fe2+ and Fe3+ ions on human plasma cholinesterase activity were investigated.
  
  
  METHODS
  Plasma were obtained (n = 24) from healthy male volunteers, and treated with five various concentrations of Fe2+ and Fe3+ ions. Activity of butyryl cholinesterase (BChE) was assayed according to colorimetric EIIman procedure. The activity was calculated as micromole of hydrolyzed substrate, per minute in each ml.
  
  
  RESULTS
  Both ferric (Fe3+) and ferrous (Fe2+) ions significantly decreased the BChE activity in all tested concentrations. The effect of ferric (Fe3+) ions on the reduction of plasma enzyme activity was more pronounced than ferrous (Fe2+) ions (p < 0.001, respect to control group).
  
  
  CONCLUSIONS
  The present paper represents the effect of Fe2+ and Fe3+ ions on ButyrylChE activity in human plasma. It reveals that both ions suppress BChE activity.
authors.affiliation: Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Karami
authors.forename: M A
authors.initials: MA
authors.lastname: Ebrahimzadeh
authors.forename: M R
authors.initials: MR
authors.lastname: Mahdavi
authors.forename: A
authors.initials: A
authors.lastname: Kazemi
mesh_terms: D000328:Adult; D002091:Butyrylcholinesterase / Q000097:blood*; D005290:Ferric Compounds / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006801:Humans; D008297:Male
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D002091:Butyrylcholinesterase