title: Risks associated with commodity trade: transmission of viral haemorrhagic septicaemia virus (VHSV) to rainbow trout fry from VHSV-carrying tissue-homogenates.
abstract: Movements of commodity fish present a potential risk of transferring pathogens. Within a study to estimate the risk from imported rainbow trout Oncorhynchus mykiss carcases, fry were exposed to tissue homogenates from market size rainbow trout infected experimentally with viral haemorrhagic septicaemia (VHS) by waterborne exposure to VHS virus (VHSV, isolate of genotype Ia). Tissues were collected from fish that showed clinical signs and from recent mortalities. Homogenates of (i) internal organs, (ii) brain/gills and (iii) muscle tissue were prepared and added to tanks holding the fry. Virus transmission occurred from all tissues tested, causing high mortality of the fry. The results underline the potential risk of introduction of VHSV through the trade of fish products.
authors.affiliation: Cefas Weymouth Laboratory, Barrack Road, Weymouth, Dorset, UK. Birgit.Oidtmann@cefas.co.uk
authors.forename: B
authors.initials: B
authors.lastname: Oidtmann
authors.forename: C
authors.initials: C
authors.lastname: Joiner
authors.forename: R A
authors.initials: RA
authors.lastname: Reese
authors.forename: D
authors.initials: D
authors.lastname: Stone
authors.forename: M
authors.initials: M
authors.lastname: Dodge
authors.forename: P
authors.initials: P
authors.lastname: Dixon
mesh_terms: D000818:Animals; D003132:Commerce; D017931:DNA Primers; D004195:Disease Models, Animal; D029141:Novirhabdovirus / Q000235:genetics / Q000302:isolation & purification*; D017686:Oncorhynchus mykiss / Q000821:virology*; D018353:Rhabdoviridae Infections / Q000401:mortality / Q000635:transmission*; D018570:Risk Assessment; D014871:Water Microbiology
chemical_list: D017931:DNA Primers