title: [Progress of isolation and purification of pancreatic islets].
abstract: OBJECTIVE
  To review the general approaches in isolation and purification of pancreatic islets and progress in several aspects.
  
  
  METHODS
  The latest literature concerning acquisition of pancreatic islets was reviewed and analyzed in terms of the choice of pancreatic islet donors, the digestion and isolation of pancreas, the purification of islet and the assay of outcome.
  
  
  RESULTS
  The profile of the isolation and purification depends on the selection of reagents and methods of operation in every step and linkup between every step.
  
  
  CONCLUSIONS
  Pancreatic islet transplantation is the most effective method to treat type 1 diabetes, the problem of inadequate sources of pancreatic islets could be resolved by the optimal process and the establishment of standardized operation.
authors.affiliation: Department of Biotechnology, College of Chemical Engineering and Biotechnology, Hebei Polytechnic University, Tangshan Hebei 063009, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Yang
mesh_terms: D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*