title: Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix vaccine strain.
abstract: Rotaviruses are important enteric pathogens for humans and animals. Group A rotaviruses (RV-A) are the most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective method to reduce RV-A-associated diseases. G1P[8], the most prevalent RV-A genotype worldwide, is included in the RV-A vaccine Rotarix®. The discrimination between wild-type G1P[8] and vaccine G1P[8] strains is an important topic in the study of RV-A epidemiology to manage outbreaks and to define control measures for vaccinated children. In this study, we developed a novel method to segregate the wild-type and vaccine strains using restriction endonucleases. The dsRNA from the Rotarix® vaccine was sequenced and the NSP3 gene was selected as the target gene. The vaccine strain has a restriction pattern that is different than that of wild-type RV-A G1P[8] isolates after digestion with the restriction endonuclease BspHI. This pattern could be used as a marker for the differentiation of wild-type G1P[8] strains from the vaccine strain.
authors.affiliation: Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil, 21040-360. tatilrose@ioc.fiocruz.br
authors.forename: Tatiana L
authors.initials: TL
authors.lastname: Rose
authors.forename: Marize P
authors.initials: MP
authors.lastname: Miagostovich
authors.forename: José Paulo G
authors.initials: JP
authors.lastname: Leite
mesh_terms: D004262:DNA Restriction Enzymes / Q000235:genetics; D005243:Feces / Q000821:virology*; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology; D022243:Rotavirus Vaccines / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000235:genetics
chemical_list: C492457:RIX4414 vaccine; D022243:Rotavirus Vaccines; D014613:Vaccines, Attenuated; D004262:DNA Restriction Enzymes