title: Kinetic delay of cyclization/elimination-coupled enzyme assays: analysis and solution.
abstract: A kinetic analysis of an enzyme assay employing a synthetic substrate that produces a detectable signal through a spontaneous organic cyclization/elimination reaction following the enzymatic reaction was conducted. The results from the calculation were used to predict the lag period and provide accurate measurements of the activity of alkaline phosphatase using the fluorogenic substrate (1).
authors.affiliation: Department of Chemistry, University of Louisiana at Lafayette, Lafayette, LA 70504, USA. fxx6907@louisiana.edu
authors.forename: Fengtian
authors.initials: F
authors.lastname: Xue
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Seto
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D003500:Cyclization; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics
chemical_list: D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase