title: Microbial diversity with dominance of 16S rRNA gene sequences with high GC contents at 74 and 98 °C subsurface crude oil deposits in Japan.
abstract: We prepared DNA from the production waters of oil deposits and wellheads of the high- and hypertemperature Japanese oil wells #AR39 (depth, 1230 m; temperature, 74 °C; pressure, 2.92 MPa) and #SR123 (depth, 1687 m; temperature, 98 °C; pressure, 11.3 MPa) to detect indigenous bacterial and archaeal microorganisms. We used PCR to amplify the 16S rRNA genes of microbial communities and characterized them based on their sequences. A few species of microorganisms with high GC contents were detected in samples from oil deposits, whereas the microbial constituents and their GC contents were diverse in wellhead samples. A comparison of the composition of the microbial communities found that the predominant indigenous populations in the #SR123 oil deposit were Thermotoga hypogea-, Thermotoga petrophila- and Thermodesulfobacterium commune-like bacteria with a 61-63% GC content in their 16S rRNA gene sequences, and Archaeoglobus fulgidus-like archaea with a 65% GC content, whereas the major population in #AR39 comprised Thermacetogenium phaeum- and Fervidobacterium pennavorans-like bacteria and Methanothermobacter thermautotrophicus-like archaea with a 60%, 60% and 61% GC content, respectively.
authors.affiliation: National Food Research Institute, Tsukuba-Shi, Ibaraki, Japan. yamane.kunio.fb@u.tsukuba.ac.jp
authors.forename: Kunio
authors.initials: K
authors.lastname: Yamane
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hattori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohtagaki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fujiwara
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics*; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001482:Base Composition; D058448:Biota; D015723:Gene Library; D007564:Japan; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014867:Water / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D010578:Petroleum; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014867:Water