title: Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity.
abstract: To determine whether purified Ascaris suum haemoglobin (AsHb) is a suitable vaccine candidate for the control of Ascaris infections, pigs were vaccinated with AsHb in combination with QuilA adjuvant and challenged with A. suum eggs. The number of liver lesions and worms in the intestine was assessed on day 14, 28 and 56 post-infection (p.i.). No significant differences were found in the number of worms recovered between vaccinated and control pigs on any of these days. However, significantly more white spots were counted on the livers of vaccinated pigs on day 14 (+86%) and day 28 (+118%) p.i. compared with nonvaccinated controls. To investigate whether the increased immunoreactivity against the liver stage L3s in vaccinated pigs was triggered by and directed against AsHb, the transcription and expression of AsHb in this larval life stage was analysed by RT-PCR and immunoblotting. The results showed that neither the AsHb transcript nor protein was detectable in freshly hatched L3. However, the immunoblot analysis showed that vaccination with AsHb resulted in the production of antibodies binding to several other antigens of the L3, suggesting that these might be involved in the increased white spot development.
authors.affiliation: Department of Virology, Parasitology and Immunology, Ghent University. Salisburylaan 133, Merelbeke, Belgium. johnny.vlaminck@ugent.be
authors.forename: J
authors.initials: J
authors.lastname: Vlaminck
authors.forename: M
authors.initials: M
authors.lastname: Martinez-Valladares
authors.forename: S
authors.initials: S
authors.lastname: Dewilde
authors.forename: L
authors.initials: L
authors.lastname: Moens
authors.forename: K
authors.initials: K
authors.lastname: Tilleman
authors.forename: D
authors.initials: D
authors.lastname: Deforce
authors.forename: J
authors.initials: J
authors.lastname: Urban
authors.forename: E
authors.initials: E
authors.lastname: Claerebout
authors.forename: J
authors.initials: J
authors.lastname: Vercruysse
authors.forename: P
authors.initials: P
authors.lastname: Geldhof
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D017165:Ascaris suum / Q000276:immunology* / Q000472:pathogenicity; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D006454:Hemoglobins / Q000276:immunology* / Q000633:toxicity; D008099:Liver / Q000473:pathology*; D062605:Quillaja Saponins; D012503:Saponins / Q000008:administration & dosage; D013552:Swine; D014612:Vaccines / Q000009:adverse effects* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D006454:Hemoglobins; D062605:Quillaja Saponins; D012503:Saponins; D014612:Vaccines; C046386:Quil A