title: The mimivirus R355 gene product: preliminary crystallographic analysis of a putative ubiquitin-like protein-specific protease.
abstract: The complete genome sequence of the largest known double-stranded DNA virus, mimivirus, reveals the presence of a gene (denoted R355) that potentially encodes a cysteine protease that is expressed late (after 6 h) in the infectious cycle of the virus. In order to verify a sequence-based functional prediction and understand its role during the infectious process, the R355 protein was produced to assay its proteolytic activity and solve its three-dimensional structure. Here, the preliminary crystallographic analysis of the recombinant viral protein is reported. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with a monomer in the asymmetric unit. A MAD data set was used for preliminary phasing using the selenium signal from a selenomethionine-substituted protein crystal.
authors.affiliation: Information Génomique et Structurale, CNRS UPR 2589, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université, 163 Avenue de Luminy, Case 934, 13288 Marseille CEDEX 09, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Jeudy
authors.forename: Audrey
authors.initials: A
authors.lastname: Lartigue
authors.forename: Pascal
authors.initials: P
authors.lastname: Mansuelle
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ogata
authors.forename: Chantal
authors.initials: C
authors.lastname: Abergel
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics; D056686:Mimiviridae / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D064570:Ubiquitin-Specific Proteases; D014452:Ubiquitins / Q000737:chemistry* / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D014452:Ubiquitins; D014764:Viral Proteins; D010450:Endopeptidases; D064570:Ubiquitin-Specific Proteases