title: [Effect of the cryopreserved Schwann cells on the peripheral nerve regeneration].
abstract: OBJECTIVE
  To compare the effects of primary culture Schwann cells and cryopreserved Schwann cells on the injured peripheral nerve regeneration.
  
  
  METHODS
  The primary culture Schwann cells and cryopreserved Schwann cells were transplanted into the silicone tubes that bridged the resected sciatic nerves. At different time after transplantation, HRP was injected into the sciatic nerve trunks at the distal end of the silicone tubes, and then the HRP labeled neurons in dorsal root ganglions and anterior horns of spinal cord were counted. The complex action potential of regenerated nerve fibers was measured. Myelination on regenerated nerve fibers was investigated under electronic microscope.
  
  
  RESULTS
  At different time after transplantation, there were not significant differences that the HRP labeled neurons in dorsal root ganglions and anterior horns of spinal cord, the complex action potential of regenerated nerve fibers and myelination on regenerated nerve fibers (P > 0.05), between primary culture and cryopreserved Schwann cells.
  
  
  CONCLUSIONS
  The cryopreserved Schwann cells still play the same important role in the regeneration of injured peripheral nerves as the primary culture Schwann cells do.
authors.affiliation: Department of Physiology, Shantou University Medical College, Shantou 515031, China.
authors.forename: Guang-You
authors.initials: GY
authors.lastname: Feng
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Chang
mesh_terms: D000200:Action Potentials; D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation; D009416:Nerve Regeneration / Q000502:physiology*; D010525:Peripheral Nerves / Q000502:physiology*; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology