title: Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach.
abstract: OBJECTIVE
  Parkinson's disease (PD) ranks the second among the neurodegenerative disorders. Proteins involved in Parkinson's disease (PD) have been investigated but none as the diagnostic markers in blood.
  
  
  METHODS
  In this study, we applied a proteomic strategy, by utilizing two-dimensional electrophoresis and mass spectrometry, to analyze two sample pools of plasma from the healthy individuals and PD subjects.
  
  
  RESULTS
  IgGκL and human serum amyloid P component (SAP) were found differentially expressed between these pools. SAP level increased by approximately 5-fold in PD samples, and the ELISA procedure revealed a significant (P<0.001) increase in SAP concentration (65.9 ± 18.7μg/mL) in the plasma of PD subjects (healthy individuals, 35.0 ± 12.5μg/mL), with sensitivity of 94.1% and specificity of 87.5%.
  
  
  CONCLUSIONS
  Our results indicated a potential feasibility of plasma SAP as a marker to approach PD.
authors.affiliation: Graduate Institute of Applied Science and Engineering, Fu-Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Chen
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Vinchi
authors.initials: V
authors.lastname: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010300:Parkinson Disease / Q000097:blood / Q000175:diagnosis*; D040901:Proteomics; D000683:Serum Amyloid P-Component / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D000683:Serum Amyloid P-Component