title: Inhibition of gene expression in Escherichia coli under hypergravity.
abstract: We investigated the effects of hypergravity on gene expression in Escherichia coli. Analysis of gene expression using green fluorescence protein reporter showed downregulation at 50,000 g, as found for OmpW, which was one of the gravity-induced proteins. Real time-PCR analysis showed normal transcription even at 50,000 g. These results suggest that hypergravity inhibited the translation process in gene expression.
authors.affiliation: Bio-Nano Electronics Research Center, Toyo University, 2100 Kujirai, Kawagoe, Saitama 350-8585, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Shimoshige
authors.forename: Hideki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D048168:Escherichia coli K12 / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D018471:Hypergravity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; C064035:ompW protein, E coli