title: Heterologous expression of Aspergillus oryzae xylose reductase and xylitol dehydrogenase genes facilitated xylose utilization in the yeast Saccharomyces cerevisiae.
abstract: The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.
authors.affiliation: Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Jitsuro
authors.initials: J
authors.lastname: Kaneda
authors.forename: Kenji
authors.initials: K
authors.lastname: Sasaki
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shintani
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics*; D050542:D-Xylulose Reductase / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism; D015870:Gene Expression; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D000431:Ethanol; D014994:Xylose; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase