title: Evaluation of PMI-5011, an ethanolic extract of Artemisia dracunculus L., on peripheral neuropathy in streptozotocin-diabetic mice.
abstract: We previously reported that PMI-5011, an ethanolic extract of Artemisia dracunculus L., alleviates peripheral neuropathy in high fat diet-fed mice, a model of prediabetes and obesity developing oxidative stress and pro-inflammatory changes in the peripheral nervous system. This study evaluated PMI-5011 on established functional, structural, and biochemical changes associated with Type I diabetic peripheral neuropathy. C57Bl6/J mice with streptozotocin-induced diabetes of a 12-week duration, developed motor and sensory nerve conduction velocity deficits, thermal and mechanical hypoalgesia, tactile allodynia, and intra-epidermal nerve fiber loss. PMI-5011 (500 mg/kg/day for 7 weeks) alleviated diabetes-induced nerve conduction slowing, small sensory nerve fiber dysfunction, and increased intra-epidermal nerve fiber density. PMI-5011 blunted sciatic nerve and spinal cord 12/15-lipoxygenase activation and oxidative-nitrosative stress, without ameliorating hyperglycemia or reducing sciatic nerve sorbitol pathway intermediate accumulation. In conclusion, PMI-5011, a safe and non-toxic botanical extract, may find use in the treatment of diabetic peripheral neuropathy.
authors.affiliation: Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA.
authors.forename: Pierre
authors.initials: P
authors.lastname: Watcho
authors.forename: Roman
authors.initials: R
authors.lastname: Stavniichuk
authors.forename: Pierre
authors.initials: P
authors.lastname: Tane
authors.forename: Hanna
authors.initials: H
authors.lastname: Shevalye
authors.forename: Yury
authors.initials: Y
authors.lastname: Maksimchyk
authors.forename: Pal
authors.initials: P
authors.lastname: Pacher
authors.forename: Irina G
authors.initials: IG
authors.lastname: Obrosova
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects; D004817:Epidermis / Q000294:innervation* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006930:Hyperalgesia / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006943:Hyperglycemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008084:Lipoxygenase / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012584:Sciatic Nerve / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
chemical_list: D004041:Dietary Fats; D009419:Nerve Tissue Proteins; C541449:PMI-5011; D010936:Plant Extracts; D008084:Lipoxygenase