title: Standardized "no-touch" technique for descemet membrane endothelial keratoplasty.
abstract: We describe a standardized technique for "no-touch" isolated Descemet membrane transplant, ie, Descemet membrane endothelial keratoplasty (DMEK). All essential steps, including patient preparation and descemetorhexis as well as DMEK graft implantation, orientation, unrolling, centering, appositioning, and fixation, are described in detail. In the management of Fuchs endothelial dystrophy, the technique may provide a best-corrected visual acuity of 20/25 or better (≥ 0.8) in three-quarters of cases and an endothelial cell density of about 1800 to 2000 cells/mm(2) at 6 months after surgery. No-touch DMEK may therefore be a safe and effective procedure for the treatment of corneal endothelial disorders, making endothelial keratoplasty accessible to most corneal surgeons without requiring major investments while providing an unprecedented visual rehabilitation rate and outcome.
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery, Rotterdam, the Netherlands.
authors.forename: Isabel
authors.initials: I
authors.lastname: Dapena
authors.forename: Kyros
authors.initials: K
authors.lastname: Moutsouris
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Droutsas
authors.forename: Lisanne
authors.initials: L
authors.lastname: Ham
authors.forename: Korine
authors.initials: K
authors.lastname: van Dijk
authors.forename: Gerrit R J
authors.initials: GR
authors.lastname: Melles
mesh_terms: D002452:Cell Count; D057111:Descemet Stripping Endothelial Keratoplasty / Q000379:methods* / Q000592:standards; D004728:Endothelium, Corneal / Q000473:pathology / Q000637:transplantation; D005642:Fuchs' Endothelial Dystrophy / Q000503:physiopathology / Q000601:surgery*; D006801:Humans; D014792:Visual Acuity / Q000502:physiology