title: Wound healing/regeneration using recombinant human growth/differentiation factor-5 in an injectable poly-lactide-co-glycolide-acid composite carrier and a one-wall intra-bony defect model in dogs.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of recombinant human growth/differentiation factor-5 (rhGDF-5) on periodontal wound healing/regeneration using an injectable poly-lactide-co-glycolide-acid (PLGA) composite carrier and an established defect model.
  
  
  METHODS
  Bilateral 4 × 5 mm (width × depth) one-wall, critical-size, intra-bony periodontal defects were surgically created at the second and the fourth mandibular pre-molar teeth in 15 Beagle dogs. The animals were randomized to receive (using a split-mouth design; defect sites in the same jaw quadrant getting the same treatment) rhGDF-5 high dose (188 μg/defect) versus sham-surgery control (five animals), rhGDF-5 mid dose (37 μg/defect) versus carrier control (five animals), and rhGDF-5 low dose (1.8 μg/defect) versus treatment reported elsewhere (five animals). The animals were euthanized for histometric analysis following an 8-week healing interval.
  
  
  RESULTS
  Clinical healing was uneventful. The rhGDF-5/PLGA construct was easy to assemble and apply. The rhGDF-5 high dose supported significantly increased bone formation compared with the low-dose, sham-surgery, and carrier controls (p<0.05) and induced significantly increased cementum formation compared with the controls (p<0.05). Root resorption/ankylosis or other aberrant healing events were not observed.
  
  
  CONCLUSIONS
  rhGDF-5 appears to effectively support periodontal wound healing/regeneration in a dose-dependent order; the PLGA composite appears to be an effective ease-of-use candidate for carrier technology.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Cheon-Ki
authors.initials: CK
authors.lastname: Min
authors.forename: Ulf M E
authors.initials: UM
authors.lastname: Wikesjö
authors.forename: Jung-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Gyung-Joon
authors.initials: GJ
authors.lastname: Chae
authors.forename: Susanne D
authors.initials: SD
authors.lastname: Pippig
authors.forename: Patrizia
authors.initials: P
authors.lastname: Bastone
authors.forename: Chang-Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Kim
mesh_terms: D020341:Absorbable Implants; D016301:Alveolar Bone Loss / Q000601:surgery*; D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D025021:Cementogenesis / Q000187:drug effects; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D003739:Dental Cementum / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D055428:Growth Differentiation Factor 5 / Q000008:administration & dosage / Q000627:therapeutic use*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011897:Random Allocation; D011994:Recombinant Proteins; D012038:Regeneration / Q000187:drug effects; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid