title: Research perspectives and role of lactose uptake rate revealed by its study using 14C-labelled lactose in whey fermentation.
abstract: The present investigation examines the effect of pH, temperature and cell concentration on lactose uptake rate, in relation with kinetics of whey fermentation using kefir and determines the optimum conditions of these parameters. Lactose uptake rate was measured by adding (14)C-labelled lactose in whey. The results reveal the role of lactose uptake rate, being the main factor that affects the rate of fermentation, in contrast to the activity of the enzymes involved in lactose bioconversion process. Lactose uptake rate results discussion showed that mainly Ca(2+) is responsible for the reduced whey fermentation rate in comparison with fermentations using synthetic media containing lactose. Likewise, the results draw up perspectives on whey fermentation research to improve whey fermentation rate. Those perspectives are research to remove Ca(2+) from whey, the use of nano and microtubular biopolymers and promoters such as γ-alumina pellets and volcan foaming rock kissiris in order to accelerate whey fermentation.
authors.affiliation: Food Biotechnology Group, Department of Chemistry, University of Patras, GR-26500 Patras, Greece.
authors.forename: Aristidis
authors.initials: A
authors.lastname: Golfinopoulos
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Kopsahelis
authors.forename: Konstantina
authors.initials: K
authors.lastname: Tsaousi
authors.forename: Athanasios A
authors.initials: AA
authors.lastname: Koutinas
authors.forename: Magdalini
authors.initials: M
authors.lastname: Soupioni
mesh_terms: D000704:Analysis of Variance; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D002118:Calcium / Q000302:isolation & purification; D002250:Carbon Radioisotopes / Q000378:metabolism*; D043302:Cultured Milk Products / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D008894:Milk Proteins / Q000378:metabolism*; D012588:Scintillation Counting; D013696:Temperature; D000067816:Whey Proteins; D015003:Yeasts
chemical_list: D002250:Carbon Radioisotopes; D008894:Milk Proteins; D000067816:Whey Proteins; D007785:Lactose; D002118:Calcium