title: [Effect of no mediator on kainic acid induced behavioral seizures in rats].
abstract: OBJECTIVE
  To further explore the roles of endogenous nitric oxide (NO) or NO derivatives in complex partial seizures and generalized convulsions.
  
  
  METHODS
  The effect of pretreatment with L-nitroarginine (L-NNA), an inhibitor of nitric oxide synthase (NOS), or L arginine (L-Arg), a precursor of NO on kainic acid (KA)-induced seizure in rats and the changes in the concentration of NO2 -/NO- in the hippocampus were determined.
  
  
  RESULTS
  The rats appeared with wet dog shakes (WDS) at 15 min and then occurred generalized convulsions during 1 h to 3 h after administration of KA (10 mg/kg i.p.). However, the pretreatment of L-NNA (50 mg/kg) so dramatically promoted and enhanced KA-induced behavioral seizures that the latency of generalized convulsion was shorten dramatically, and the mortality was greatly high. In contrast, the pretreatment with L-Arg (40 mg/kg) markedly delayed or weakened KA-induced behavioral changes, such as increasing latency of WDS and generalized convulsion, shortening time o f seizure and none of animal died during observed time. The concentration of NO2- /NO3- in the hippocampus increased immediately at 30 min and remained to 7 d after the administration of KA. Compared with control group (pretreatment with NS), the concentration of NO2- / NO3- in the hippocampus apparently increased at 3 h and 3 d after the administration of KA in the rats with L-Arg pretreatment.
  
  
  CONCLUSIONS
  The endogenous NO (NO or NO derivatives) mediators may play an important role against excitotoxin induced seizures in rats.
authors.affiliation: Institute for Brain Disorder, Dalian Medical University, Dalian 116027, China.
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Chang-kai
authors.initials: CK
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Da-yue
authors.initials: DY
authors.lastname: Han
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: De-zheng
authors.initials: DZ
authors.lastname: Gong
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D007608:Kainic Acid / Q000009:adverse effects*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019335:Nitroarginine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
chemical_list: D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D007608:Kainic Acid