title: Metabolites from inhalation of aerosolized S-8 synthetic jet fuel in rats.
abstract: Alternative fuels are being considered for civilian and military uses. One of these is S-8, a replacement jet fuel synthesized using the Fischer-Tropsch process, which contains no aromatic compounds and is mainly composed of straight and branched alkanes. Metabolites of S-8 fuel in laboratory animals have not been identified. The goal of this study was to identify metabolic products from exposure to aerosolized S-8 and a designed straight-chain alkane/polyaromatic mixture (decane, undecane, dodecane, tridecane, tetradecane, pentadecane, naphthalene, and 2-methylnaphthalene) in male Fischer 344 rats. Collected blood and tissue samples were analyzed for 70 straight and branched alcohols and ketones ranging from 7 to 15 carbons. No fuel metabolites were observed in the blood, lungs, brain, and fat following S-8 exposure. Metabolites were detected in the liver, urine, and feces. Most of the metabolites were 2- and 3-position alcohols and ketones of prominent hydrocarbons with very few 1- or 4-position metabolites. Following exposure to the alkane mixture, metabolites were observed in the blood, liver, and lungs. Interestingly, heavy metabolites (3-tridecanone, 2-tridecanol, and 2-tetradecanol) were observed only in the lung tissues possibly indicating that metabolism occurred in the lungs. With the exception of these heavy metabolites, the metabolic profiles observed in this study are consistent with previous studies reporting on the metabolism of individual alkanes. Further work is needed to determine the potential metabolic interactions of parent, primary, and secondary metabolites and identify more polar metabolites. Some metabolites may have potential use as biomarkers of exposure to fuels.
authors.affiliation: Interdisciplinary Toxicology Program, University of Georgia, Athens, GA, USA. raphaeltremblay@globalchemicalconsulting.com
authors.forename: Raphael T
authors.initials: RT
authors.lastname: Tremblay
authors.forename: Sheppard A
authors.initials: SA
authors.lastname: Martin
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Fisher
mesh_terms: D000473:Alkanes / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers; D005243:Feces; D006838:Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D001239:Inhalation; D019570:Inhalation Exposure / Q000009:adverse effects*; D007659:Ketones / Q000097:blood* / Q000652:urine*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000473:Alkanes; D015415:Biomarkers; D006838:Hydrocarbons; D007659:Ketones; C534345:S-8 fuel