title: NANOG has a role in mesenchymal stem cells' immunomodulatory effect.
abstract: It is well known that terminally differentiated cells derived from mesenchymal stem cells (MSCs) will lose the immunomodulation capacity. NANOG is known to be a core transcription factor in the maintenance of stem cell specific features or stemness. To evaluate whether NANOG was involved in the immunomodulation effects of MSCs, MSCs' immunomodulation capacity on lymphocyte activation and proliferation before or after endogenous NANOG interference was investigated. We found that MSCs' inhibitory effects on lymphocyte activation and proliferation was significantly weakened after NANOG knockdown. In addition, NANOG RNAi and chromatin immunoprecipitation experiments showed that NANOG suppressed the expression and secretion of DKK-1, transforming growth factor-beta1 (TGF-β1), TGF-β2, and TGF-β3, which are all important factors mediating MSCs' immunomodulation capacity. Based on these data, we propose that NANOG plays an important role in maintaining the immunomodulation functions of MSCs by regulating the expression and secretion of TGF-β1, TGF-β2, TGF-β3, and DKK-1.
authors.affiliation: Institute of Basic Medical Sciences and School of Basic Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Yashu
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Lianming
authors.initials: L
authors.lastname: Liao
authors.forename: Chunjing
authors.initials: C
authors.lastname: Bian
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Changshun
authors.initials: C
authors.lastname: Shao
authors.forename: Robert Chunhua
authors.initials: RC
authors.lastname: Zhao
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001483:Base Sequence; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D056747:Immunomodulation; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D000071317:Nanog Homeobox Protein; D034622:RNA Interference; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D000071317:Nanog Homeobox Protein; D016212:Transforming Growth Factor beta; D000099192:CD69 Antigens; C501126:DKK1 protein, human; C508017:IL2RA protein, human; C486780:NANOG protein, human