title: Optimization of the extraction conditions of the volatile compounds from chili peppers by headspace solid phase micro-extraction.
abstract: A method involving headspace-solid phase micro-extraction (HS-SPME), gas chromatography with flame ionization detection (GC-FID) and gas chromatography with mass spectrometry (GC-MS) was developed and optimized to investigate the volatile composition of Capsicum chili peppers. Five SPME fibers were tested for extraction: carboxen/polydimethylsiloxane (CAR/PDMS-75μm), polydimethylsiloxane (PDMS-100μm), divinylbenzene/polydimethylsiloxane (DVB/PDMS-65μm), carbowax/divinylbenzene (CW/DVB-70μm), and divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS-50/30μm), the last of which was shown to be the most efficient fiber to trap the volatile compounds. Optimization of the extraction conditions was carried out using multivariate strategies such as factorial design and response surface methodology. Eighty three compounds were identified by GC-MS when using the optimized extraction conditions, the majority of which were esters.
authors.affiliation: Department of Food Science, Faculty of Food Engineering, Campinas State University, Campinas, SP, Brazil.
authors.forename: Stanislau Bogusz
authors.initials: SB
authors.lastname: Junior
authors.forename: Arlete
authors.initials: A
authors.lastname: de Marchi Tavares de Melo
authors.forename: Cláudia Alcaraz
authors.initials: CA
authors.lastname: Zini
authors.forename: Helena Teixeira
authors.initials: HT
authors.lastname: Godoy
mesh_terms: D000704:Analysis of Variance; D002212:Capsicum / Q000737:chemistry*; D005410:Flame Ionization; D006358:Hot Temperature; D016018:Least-Squares Analysis; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D055549:Volatile Organic Compounds