title: Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine.
abstract: We present a method based on monolitic spin column extraction and gas chromatography-mass spectrometry as an analytical method for screening diquat (DQ), paraquat (PQ), and fenitrothion in serum and urine. This method is useful for clinical and forensic toxicological analyses. Recovery of DQ, PQ, and fenitrothion from serum and urine, spiked at concentrations between 0.1, 2.5, 20, and 45 μg/ml, ranged from 51.3% to 106.1%. Relative standard deviation percentages were between 3.3% and 14.8%. Detection and quantitation limits for serum and urine were 0.025 and 0.05 μg/ml, respectively, for DQ, 0.1 and 0.1 μg/ml, respectively, for PQ, and 0.025 and 0.05 μg/ml, respectively, for fenitrothion. Therefore, these compounds can be detected and quantified in the case of acute poisoning.
authors.affiliation: Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Shimokasuya 143, Isehara, Kanagawa 259-1143, Japan. saito@is.icc.u-tokai.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukushima
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yui
authors.forename: Shota
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Namera
authors.forename: Sadaki
authors.initials: S
authors.lastname: Inokuchi
mesh_terms: D004178:Diquat / Q000032:analysis* / Q000097:blood / Q000652:urine; D005278:Fenitrothion / Q000032:analysis* / Q000097:blood / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D010269:Paraquat / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D004178:Diquat; D010269:Paraquat; D005278:Fenitrothion