title: Feasibility study of high-temperature thermoseed inductive hyperthermia in melanoma treatment.
abstract: Current treatment modalities for melanoma do not offer satisfactory efficacy. We have developed a new, minimally invasive hyperthermia technology based on radio-frequency hyperthermia. Herein, we investigated the feasibility of using a nickel-copper thermoseed for inductive hyperthermia at a relatively high temperature (46-55 ˚C). In vitro, the thermoseed showed good thermal effects and effective killing of B16/F10 melanoma cells. Temperatures of 53.1 ± 0.5 ˚C were achieved for a single thermoseed and 56.5 ± 0.5 ˚C for two in parallel (spacing 5 mm). No B16/F10 melanoma cells survived with heating time longer than 20 min in the parallel thermoseed group. Magnetic fields or thermoseeds alone did not affect the survival rate of B16/F10 cells (P>0.05). In vivo, B16/F10 melanoma cells were subcutaneously injected into the right axilla of C57BL/6 mice. After the tumors grew to ~11-13 mm, two thermoseeds (spacing 5 mm) were implanted into the tumors and the mice were subjected to an alternating magnetic field (100-250 kHz, 15 kA/m) to induce hyperthermia. The temperature at the center of the tumor reached 46 ˚C at 5 min and plateaued at 50 ˚C. Thermoseed treatment produced large necrotic areas, inhibited tumor growth in 60% (6 of 10) of animals and prolonged survival time (P<0.05). Thus, with further optimization and testing, high-temperature thermoseed inductive hyperthermia may have therapeutic potential for melanoma.
authors.affiliation: Department of Biochemistry and Molecular Biology, China-Japan Friendship Hospital, Beijing 100029, PR China.
authors.forename: Qi-Sheng
authors.initials: QS
authors.lastname: Xia
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.forename: Tian-De
authors.initials: TD
authors.lastname: Zhao
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Li
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiang
authors.forename: Chuan-Ying
authors.initials: CY
authors.lastname: Geng
authors.forename: Lin
authors.initials: L
authors.lastname: Pan
authors.forename: Run-Lei
authors.initials: RL
authors.lastname: Hu
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Qi
authors.forename: Guang-Fei
authors.initials: GF
authors.lastname: Sun
authors.forename: Jin-Tian
authors.initials: JT
authors.lastname: Tang
mesh_terms: D000818:Animals; D003300:Copper; D005240:Feasibility Studies; D006358:Hot Temperature; D006979:Hyperthermia, Induced / Q000295:instrumentation*; D055909:Magnetic Field Therapy; D008297:Male; D008546:Melanoma, Experimental / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009532:Nickel; D013193:Stainless Steel; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: C062136:nickel copper alloy; D013193:Stainless Steel; D003300:Copper; D009532:Nickel