title: A double WAP domain-containing protein PmDWD from the black tiger shrimp Penaeus monodon is involved in the controlling of proteinase activities in lymphoid organ.
abstract: A homolog of mammalian secretory leucocyte proteinase inhibitor or SLPI known as a double WAP domain (DWD) protein has been found in penaeid shrimp and believed to play an important role in innate immune system of the shrimp. The PmDWD identified from the Penaeus monodon EST database was investigated for its expression under pathogen infection. Infections by Vibrio harveyi and white spot syndrome virus (WSSV) up-regulated the expression of the PmDWD, which was peaked at about 24 h post infection and, then, subsided to more or less normal level. The PmDWD was expressed in various tissues of normal, 24-h WSSV-injected and leg-amputated shrimp, predominantly in the hemocytes. The expression was dramatically increased in lymphoid organ upon WSSV infection and leg amputation. The recombinant PmDWD (rPmDWD) was not active against the commercial proteinases: trypsin, chymotrypsin, elastase and subtilisin while its mutant rPmDWD_F70R was active against the subtilisin. By using agar diffusion assay, the rPmDWD inhibited the crude proteinases from lymphoid organs of leg-amputated and WSSV-infected shrimp. It inhibited the crude proteinases from Bacillus subtilis as well. Unlike the mammalian SLPIs, the rPmDWD had no antimicrobial activity against various bacteria.
authors.affiliation: Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Pranisa
authors.initials: P
authors.lastname: Suthianthong
authors.forename: Naritsara
authors.initials: N
authors.lastname: Pulsook
authors.forename: Premruethai
authors.initials: P
authors.lastname: Supungul
authors.forename: Anchalee
authors.initials: A
authors.lastname: Tassanakajon
authors.forename: Vichien
authors.initials: V
authors.lastname: Rimphanitchayakit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001483:Base Sequence; D020869:Gene Expression Profiling; D008221:Lymphoid Tissue / Q000201:enzymology*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D010447:Peptide Hydrolases / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D016415:Sequence Alignment; D014733:Vibrio / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D053481:Secretory Leukocyte Peptidase Inhibitor; D010447:Peptide Hydrolases