title: Relative efficiencies of two air sampling methods and three culture conditions for the assessment of airborne culturable fungi in a poultry farmhouse in France.
abstract: Fungal elements represent a significant part of the biological contaminants that could be detected in the air of animal facilities. The aim of this study was to assess the relative efficiencies of two air sampling methods and three culture conditions for the quantification of airborne culturable fungi in a poultry farmhouse in France. Air samples were collected every week throughout a 15-week period. Two devices were simultaneously used-a rotative cup air sampler (CIP 10-M, Arelco, France) and an air sampler based on filtration (AirPort MD8, Sartorius, Germany). Culture of airborne viable fungi was performed on malt extract agar (ME) and dichloran glycerol-18 (DG18) at 25 or 37°C. CIP 10-M and AirPort MD8 were shown to display comparable performances but significant differences were observed between culture conditions for Aspergillus spp. (p<0.01), Scopulariopsis spp. (p=0.02) and unidentified molds (p<0.01).
authors.affiliation: ENVA, ANSES, UPEC UMR BIPAR, Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France.
authors.forename: Adélaïde
authors.initials: A
authors.lastname: Nieguitsila
authors.forename: Pascal
authors.initials: P
authors.lastname: Arné
authors.forename: Benoît
authors.initials: B
authors.lastname: Durand
authors.forename: Manjula
authors.initials: M
authors.lastname: Deville
authors.forename: Hélène
authors.initials: H
authors.lastname: Benoît-Valiergue
authors.forename: René
authors.initials: R
authors.lastname: Chermette
authors.forename: Sophie
authors.initials: S
authors.lastname: Cottenot-Latouche
authors.forename: Jacques
authors.initials: J
authors.lastname: Guillot
mesh_terms: D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis*; D000528:Alternaria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D000822:Animal Husbandry; D000818:Animals; D001230:Aspergillus / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D002963:Cladosporium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D046508:Culture Techniques; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D005602:France; D005658:Fungi / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D016533:Mycological Typing Techniques; D010407:Penicillium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D011200:Poultry