title: Metabonomic investigation of single and multiple strain Trypanosoma brucei brucei infections.
abstract: Although co-infections are common and can have important epidemiologic and evolutionary consequences, studies exploring biochemical effects of multiple-strain infections remain scarce. We studied metabolic responses of NMRI mice to Trypanosoma brucei brucei single (STIB777AE-Green1 or STIB246BA-Red1) and co-infections using a (1)H nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling strategy. All T. b. brucei infections caused an alteration in urinary biochemical composition by day 4 postinfection, characterized by increased concentrations of 2-oxoisocaproate, D-3-hydroxybutyrate, lactate, 4-hydroxyphenylacetate, phenylpyruvate, and 4-hydroxyphenylpyruvate, and decreased levels of hippurate. Although there were no marked differences in metabolic signatures observed in the mouse infected with a single or dual strain of T. b. brucei, there was a slower metabolic response in mice infected with T. b. brucei green strain compared with mice infected with either the red strain or both strains concurrently. Pyruvate, phenylpyruvate, and hippurate were correlated with parasitemia, which might be useful in monitoring responses to therapeutic interventions.
authors.affiliation: Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom. jia.li105@imperial.ac.uk
authors.forename: Jia V
authors.initials: JV
authors.lastname: Li
authors.forename: Jasmina
authors.initials: J
authors.lastname: Saric
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jürg
authors.initials: J
authors.lastname: Utzinger
authors.forename: Elaine
authors.initials: E
authors.lastname: Holmes
authors.forename: Oliver
authors.initials: O
authors.lastname: Balmer
mesh_terms: D000818:Animals; D005243:Feces / Q000737:chemistry; D005260:Female; D006801:Humans; D055442:Metabolome / Q000502:physiology*; D051379:Mice; D018512:Parasitemia; D014346:Trypanosoma brucei brucei / Q000145:classification*; D014353:Trypanosomiasis, African / Q000378:metabolism* / Q000469:parasitology* / Q000652:urine; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water