title: Reassessment of the role of plasma membrane domains in the regulation of vesicular traffic in yeast.
abstract: The Saccharomyces cerevisiae plasma membrane has been proposed to contain two stably distributed domains. One of these domains, known as MCC (membrane compartment of Can1) or eisosomes, consists of furrow-like membrane invaginations and associated proteins. The other domain, called MCP (membrane compartment of Pma1), consists of the rest of the membrane area surrounding the MCC patches. The role of this plasma membrane domain organization in endocytosis is under debate. Here we show by live-cell imaging that vesicular traffic is restricted to the MCP and the distribution of endocytic and exocytic sites within the MCP is independent of the MCC patch positions. Photobleaching experiments indicated that Can1 and Tat2, two MCC-enriched permeases, exchange quickly between the two domains. Total internal reflection fluorescence and epi-fluorescence microscopy showed that the enrichment of Can1 at the MCC persisted after addition of its substrate, whereas the enrichment of Tat2 disappeared within 90 seconds. The rates of stimulated endocytosis of Can1 as well as Tat2 were similar in both wild-type cells and pil1Δ cells, which lack the MCC. Thus, our data suggest that the enrichment of certain plasma membrane proteins in the MCC does not regulate the rate of their endocytosis.
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstraße 1, 69117 Heidelberg, Germany.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Brach
authors.forename: Tanja
authors.initials: T
authors.lastname: Specht
authors.forename: Marko
authors.initials: M
authors.lastname: Kaksonen
mesh_terms: D026905:Amino Acid Transport Systems / Q000378:metabolism*; D026923:Amino Acid Transport Systems, Basic / Q000378:metabolism*; D001692:Biological Transport; D004705:Endocytosis; D021962:Membrane Microdomains / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D026905:Amino Acid Transport Systems; D026923:Amino Acid Transport Systems, Basic; C502212:CAN1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C429825:TAT2 protein, S cerevisiae