title: Fluorometric determination of 2-oxoadipic acid, a common metabolite of tryptophan and lysine, by high-performance liquid chromatography with pre-chemical derivatization.
abstract: 2-Oxoadipic acid, a key metabolite of tryptophan and lysine, reacted with 1,2-diamino-4,5-methylenebenzene in an acidic solution to produce a fluorescent derivative. The reaction product was separated using a Tosoh ODS-80Ts column with 20 mmol/L of KH₂PO₄-K₂HPO₄ buffer (pH 7.0) containing 26% methanol at a flow rate 0.8 mL/min. The excitation wavelength of detection was 367 nm, and the emission wavelength was 446 nm. The limit of quantification was 1 pmol per injection, sufficiently sensitive for the determination of 2-oxoadipic acid in human and experimental animal urine.
authors.affiliation: Department of Food Science and Nutrition, School of Human Cultures, The University of Shiga Prefecture, 2500 Hassaka, Hikone, Shiga 522-8533, Japan. kshibata@shc.usp.ac.jp
authors.forename: Katsumi
authors.initials: K
authors.lastname: Shibata
authors.forename: Miki
authors.initials: M
authors.lastname: Yasui
authors.forename: Mitsue
authors.initials: M
authors.lastname: Sano
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fukuwatari
mesh_terms: D000272:Adipates / Q000737:chemistry* / Q000378:metabolism / Q000652:urine*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D051381:Rats; D014364:Tryptophan / Q000378:metabolism*; D016482:Urinalysis / Q000379:methods*
chemical_list: D000272:Adipates; D014364:Tryptophan; C001918:alpha-ketoadipic acid; D008239:Lysine