title: Interleukin 1β-induced synthesis and secretion of prostaglandin E₂ in the porcine uterus during various periods of pregnancy and the estrous cycle.
abstract: Peri-implantation porcine embryos express interleukin-1β (IL-1β), which could affect uterine activity during early pregnancy. In vitro studies were conducted to determine if IL-1β stimulates secretion of PGE₂ and expression of cyclooxygenase-2 (COX-2) mRNA and microsomal PGE synthase-1 (mPGES-1) mRNA in uterine tissues harvested from pigs on days 10 to 11, 12 to 13 and 15 to 16 of pregnancy and the estrous cycle. IL-1β (10 ng/ml) increased PGE₂ secretion and mPGES-1 mRNA expression in uterine tissues isolated from pigs between days 10 to 13 of pregnancy and the estrous cycle. IL-1β stimulated PGE₂ and mPGES-1 mRNA expression only in cyclic uterine tissues on days 15 to 16. Interleukin-1β increased COX-2 mRNA expression in the endometrial tissues of pregnant and cyclic pigs harvested on days 10 to 13. It stimulated COX-2 expression in pregnant pigs' myometrial tissues on days 10 to 11, and on days 15 to 16 in tissues from both pregnant and cyclic pigs. The uterine secretion of PGE₂ in response to IL-1β was determined by local intrauterine concentrations of P₄ and E₂. This study demonstrates that IL-1β activates expression of mPGES-1 mRNA in uterine tissues to stimulate synthesis and secretion of PGE₂ on days 10 to 13 of both pregnancy and the estrous cycle. The profile of COX-2 mRNA expression in the myometrium differs from its profile in the endometrium. This work provides new insight on the role of IL-1β in PGE₂ production to overcome luteolysis in pregnant pigs.
authors.affiliation: Department of Animal Physiology, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. anitaf@uwm.edu.pl
authors.forename: A
authors.initials: A
authors.lastname: Franczak
authors.forename: A
authors.initials: A
authors.lastname: Zmijewska
authors.forename: B
authors.initials: B
authors.lastname: Kurowicka
authors.forename: B
authors.initials: B
authors.lastname: Wojciechowicz
authors.forename: G
authors.initials: G
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics; D015232:Dinoprostone / Q000096:biosynthesis* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000378:metabolism; D004958:Estradiol / Q000097:blood / Q000378:metabolism; D030762:Estrous Cycle / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D053583:Interleukin-1beta / Q000494:pharmacology*; D019746:Intramolecular Oxidoreductases / Q000235:genetics; D003341:Luteolysis / Q000187:drug effects; D009215:Myometrium / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000097:blood / Q000378:metabolism; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000096:biosynthesis; D013552:Swine; D013997:Time Factors; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D051546:Cyclooxygenase 2; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone