title: Characterization of the plant cell wall proteome using high-throughput screens.
abstract: Plant cell wall proteins play essential roles in many important biological processes, and yet there is still not a comprehensive catalogue of the cell wall proteome, or "secretome". Here, we describe three procedures, including a yeast secretion trap (YST), Agrobacterium-mediated transient expression using a necrosis-inducing protein (NIP) and protein localization assay using a fluorescent protein, to identify and confirm the localization of cell wall proteins. The approaches are orthogonal and collectively provide a powerful suite of approaches to complement more commonly used strategies to isolate plant cell wall-associated proteins.
authors.affiliation: Department of Plant Biology, Cornell University, Ithaca, NY, USA.
authors.forename: Sang-Jik
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jocelyn K C
authors.initials: JK
authors.lastname: Rose
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D019470:Biolistics; D002473:Cell Wall / Q000737:chemistry*; D015723:Gene Library; D005822:Genetic Vectors; D057166:High-Throughput Screening Assays; D018613:Microscopy, Confocal; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D020543:Proteome / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D010940:Plant Proteins; D020543:Proteome; D011993:Recombinant Fusion Proteins