title: New integrated elemental and molecular strategies as a diagnostic tool for the quality of water soluble quantum dots and their bioconjugates.
abstract: Herein, we demonstrate that both qualitative molecular and quantitative elemental data obtained from size exclusion chromatography coupled online for the first time to both molecular fluorescence and elemental mass spectrometry, respectively, turned out to be critical to evaluate the quality of coatings of quantum dots. Moreover, such an instrumental approach also allowed us to study quantitatively the appropriated bioconjugation of quantum dots to antibodies, a critical step for QDs future use in quantitative fluorescence immunoassays.
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería, 8, 33006, Oviedo, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Trapiella-Alfonso
authors.forename: Antonio R
authors.initials: AR
authors.lastname: Montoro Bustos
authors.forename: Jorge Ruiz
authors.initials: JR
authors.lastname: Encinar
authors.forename: Jose M
authors.initials: JM
authors.lastname: Costa-Fernández
authors.forename: Rosario
authors.initials: R
authors.lastname: Pereiro
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sanz-Medel
mesh_terms: D015200:Fluoroimmunoassay / Q000379:methods*; D045663:Quantum Dots; D012995:Solubility; D013050:Spectrometry, Fluorescence / Q000379:methods*; D018511:Systems Integration; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water