title: Bone cell-materials interactions and Ni ion release of anodized equiatomic NiTi alloy.
abstract: A laser processed NiTi alloy was anodized for different times in H(2)SO(4) electrolyte with varying pH to create biocompatible surfaces with low Ni ion release as well as bioactive surfaces to enhance biocompatibility and bone cell-material interactions. The anodized surfaces were assessed for their in vitro cell-material interactions using human fetal osteoblast (hFOB) cells for 3, 7 and 11 days, and Ni ion release up to 8 weeks in simulated body fluids. The results were correlated with the surface morphologies of anodized surfaces characterized using field-emission scanning electron microscopy (FESEM). The results show that anodization creates a surface with nano/micro-roughness depending on the anodization conditions. The hydrophilicity of the NiTi surface was found to improve after anodization, as shown by the lower contact angles in cell medium, which dropped from 32° to <5°. The improved wettability of anodized surfaces is further corroborated by their high surface energy, comparable with that of commercially pure Ti. Relatively high surface energies, especially the polar component, and nano/micro surface features of anodized surfaces significantly increased the number of living cells and their adherence and growth on these surfaces. Finally, a significant drop in Ni ion release from 268±11 to 136±15 ppb was observed for NiTi surfaces after anodization. This work indicates that anodization of a NiTi alloy has a positive influence on the surface energy and surface morphology, which in turn improves bone cell-material interactions and reduces Ni ion release in vitro.
authors.affiliation: School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Sheldon A
authors.initials: SA
authors.lastname: Bernard
authors.forename: Vamsi Krishna
authors.initials: VK
authors.lastname: Balla
authors.forename: Neal M
authors.initials: NM
authors.lastname: Davies
authors.forename: Susmita
authors.initials: S
authors.lastname: Bose
authors.forename: Amit
authors.initials: A
authors.lastname: Bandyopadhyay
mesh_terms: D001842:Bone and Bones / Q000166:cytology*; D002152:Calorimetry, Differential Scanning; D002450:Cell Communication / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D004566:Electrodes; D004573:Electrolytes / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007477:Ions; D008855:Microscopy, Electron, Scanning; D009532:Nickel / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D013816:Thermodynamics; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D004573:Electrolytes; D007477:Ions; C040654:titanium nickelide; D009532:Nickel; D014025:Titanium