title: The selection and use of sorghum (Sorghum propinquum) bacterial artificial chromosomes as cytogenetic FISH probes for maize (Zea mays L.).
abstract: The integration of genetic and physical maps of maize is progressing rapidly, but the cytogenetic maps lag behind, with the exception of the pachytene fluorescence in situ hybridization (FISH) maps of maize chromosome 9. We sought to produce integrated FISH maps of other maize chromosomes using Core Bin Marker loci. Because these 1 Kb restriction fragment length polymorphism (RFLP) probes are below the FISH detection limit, we used BACs from sorghum, a small-genome relative of maize, as surrogate clones for FISH mapping. We sequenced 151 maize RFLP probes and compared in silico BAC selection methods to that of library filter hybridization and found the latter to be the best. BAC library screening, clone verification, and single-clone selection criteria are presented along with an example of transgenomic BAC FISH mapping. This strategy has been used to facilitate the integration of RFLP and FISH maps in other large-genome species.
authors.affiliation: Department of Biological Science, The Florida State University, Tallahassee, FL 32306-4370, USA.
authors.forename: Debbie M
authors.initials: DM
authors.lastname: Figueroa
authors.forename: James D
authors.initials: JD
authors.lastname: Davis
authors.forename: Cornelia
authors.initials: C
authors.lastname: Strobel
authors.forename: Maria S
authors.initials: MS
authors.lastname: Conejo
authors.forename: Katherine D
authors.initials: KD
authors.lastname: Beckham
authors.forename: Brian C
authors.initials: BC
authors.lastname: Ring
authors.forename: Hank W
authors.initials: HW
authors.lastname: Bass
mesh_terms: D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D015342:DNA Probes / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000378:metabolism; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020161:Physical Chromosome Mapping; D030821:Plants, Genetically Modified; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D045868:Sorghum / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D004996:Ethidium