title: Near-IR single fluorophore quenching system based on phthalocyanine (Pc) aggregation and its application for monitoring inhibitor/activator action on a therapeutic target: L1-EN.
abstract: Controlled H-aggregation of single Pc-labeled oligonucleotides is utilized as a fluorescence quenching system to discern changes in enzyme activity for the discovery of inhibitors for Long Interspersed Element 1 endonuclease (L1-EN), which is involved in genome instability and implicated in many different diseases.
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Nesterova
authors.forename: Cecily A
authors.initials: CA
authors.lastname: Bennett
authors.forename: S Sibel
authors.initials: SS
authors.lastname: Erdem
authors.forename: Robert P
authors.initials: RP
authors.lastname: Hammer
authors.forename: Prescott L
authors.initials: PL
authors.lastname: Deininger
authors.forename: Steven A
authors.initials: SA
authors.lastname: Soper
mesh_terms: D004194:Disease / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000037:antagonists & inhibitors*; D020536:Enzyme Activators / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D042822:Genomic Instability; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D020084:Long Interspersed Nucleotide Elements; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D020536:Enzyme Activators; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D009841:Oligonucleotides; D004706:Endodeoxyribonucleases; C013647:phthalocyanine