title: ERCC1 is not expressed in hepatocellular cancer: A turkish oncology group, gastrointestinal oncology subgroup study.
abstract: OBJECTIVE
  hepatocellular cancer (HCC) is a common malignancy with a high mortality rate. Existence of excisional repair cross complementation1 (ERCC1) is implicated in resistance to cisplatin treatment. Expression of ERCC1 in HCC is not known. In this study we aimed to find out whether a subset of HCC patients can be identified to benefit from cisplatin.
  
  
  METHODS
  sixty-one patients with HCC who had enough tissue to do immunohistochemistry were identified in 3 institutions. Immunohistochemical staining was performed manually using the standard streptavidin-biotin-peroxidase method. Monoclonal anti-ERCC 1 (D-10) antibody from Santa Cruz Biotechnology (Santa Cruz, CA) was used.
  
  
  RESULTS
  only one out of 61 patients (1.6%) had ERCC1 expression.
  
  
  CONCLUSIONS
  although around 10% of HCC patients respond to cisplatin, this is unlikely to be due to ERCC1 negativity. Pathways other than ERCC1 should be searched to find ways to help these patients' treatment strategies.
authors.affiliation: Marmara University School of Medicine, Department of Medical Oncology, Istanbul, Turkey. turhal@superonline.com
authors.forename: N S
authors.initials: NS
authors.lastname: Turhal
authors.forename: E
authors.initials: E
authors.lastname: Bas
authors.forename: O
authors.initials: O
authors.lastname: Er
authors.forename: M
authors.initials: M
authors.lastname: Aliustaoglu
authors.forename: S
authors.initials: S
authors.lastname: Seber
authors.forename: F
authors.initials: F
authors.lastname: Dane
authors.forename: T
authors.initials: T
authors.lastname: Korkmaz
authors.forename: I
authors.initials: I
authors.lastname: Soyuer
authors.forename: S
authors.initials: S
authors.lastname: Ozkara
authors.forename: C
authors.initials: C
authors.lastname: Celikel
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D004268:DNA-Binding Proteins; C071447:ERCC1 protein, human; D004720:Endonucleases