title: A nuclear magnetic resonance-based structural rationale for contrasting stoichiometry and ligand binding site(s) in fatty acid-binding proteins.
abstract: Liver fatty acid-binding protein (LFABP) is a 14 kDa cytosolic polypeptide, differing from other family members in the number of ligand binding sites, the diversity of bound ligands, and the transfer of fatty acid(s) to membranes primarily via aqueous diffusion rather than direct collisional interactions. Distinct two-dimensional (1)H-(15)N nuclear magnetic resonance (NMR) signals indicative of slowly exchanging LFABP assemblies formed during stepwise ligand titration were exploited, without determining the protein-ligand complex structures, to yield the stoichiometries for the bound ligands, their locations within the protein binding cavity, the sequence of ligand occupation, and the corresponding protein structural accommodations. Chemical shifts were monitored for wild-type LFABP and an R122L/S124A mutant in which electrostatic interactions viewed as being essential to fatty acid binding were removed. For wild-type LFABP, the results compared favorably with the data for previous tertiary structures of oleate-bound wild-type LFABP in crystals and in solution: there are two oleates, one U-shaped ligand that positions the long hydrophobic chain deep within the cavity and another extended structure with the hydrophobic chain facing the cavity and the carboxylate group lying close to the protein surface. The NMR titration validated a prior hypothesis that the first oleate to enter the cavity occupies the internal protein site. In contrast, (1)H and (15)N chemical shift changes supported only one liganded oleate for R122L/S124A LFABP, at an intermediate location within the protein cavity. A rationale based on protein sequence and electrostatics was developed to explain the stoichiometry and binding site trends for LFABPs and to put these findings into context within the larger protein family.
authors.affiliation: Department of Chemistry, College of Staten Island, New York, NY, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Rima
authors.initials: R
authors.lastname: Estephan
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yang
authors.forename: Adriana
authors.initials: A
authors.lastname: Vela
authors.forename: Hsin
authors.initials: H
authors.lastname: Wang
authors.forename: Cédric
authors.initials: C
authors.lastname: Bernard
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Stark
mesh_terms: D000818:Animals; D001665:Binding Sites; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D019301:Oleic Acid / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D055672:Static Electricity
chemical_list: D050556:Fatty Acid-Binding Proteins; D008024:Ligands; D019301:Oleic Acid