title: The application of MRI for depiction of subtle blood brain barrier disruption in stroke.
abstract: The development of imaging methodologies for detecting blood-brain-barrier (BBB) disruption may help predict stroke patient's propensity to develop hemorrhagic complications following reperfusion. We have developed a delayed contrast extravasation MRI-based methodology enabling real-time depiction of subtle BBB abnormalities in humans with high sensitivity to BBB disruption and high spatial resolution. The increased sensitivity to subtle BBB disruption is obtained by acquiring T1-weighted MRI at relatively long delays (~15 minutes) after contrast injection and subtracting from them images acquired immediately after contrast administration. In addition, the relatively long delays allow for acquisition of high resolution images resulting in high resolution BBB disruption maps. The sensitivity is further increased by image preprocessing with corrections for intensity variations and with whole body (rigid+elastic) registration. Since only two separate time points are required, the time between the two acquisitions can be used for acquiring routine clinical data, keeping the total imaging time to a minimum. A proof of concept study was performed in 34 patients with ischemic stroke and 2 patients with brain metastases undergoing high resolution T1-weighted MRI acquired at 3 time points after contrast injection. The MR images were pre-processed and subtracted to produce BBB disruption maps. BBB maps of patients with brain metastases and ischemic stroke presented different patterns of BBB opening. The significant advantage of the long extravasation time was demonstrated by a dynamic-contrast-enhancement study performed continuously for 18 min. The high sensitivity of our methodology enabled depiction of clear BBB disruption in 27% of the stroke patients who did not have abnormalities on conventional contrast-enhanced MRI. In 36% of the patients, who had abnormalities detectable by conventional MRI, the BBB disruption volumes were significantly larger in the maps than in conventional MRI. These results demonstrate the advantages of delayed contrast extravasation in increasing the sensitivity to subtle BBB disruption in ischemic stroke patients. The calculated disruption maps provide clear depiction of significant volumes of BBB disruption unattainable by conventional contrast-enhanced MRI.
authors.affiliation: Molecular Genetics Dept., Weizmann Inst., Israel.
authors.forename: David
authors.initials: D
authors.lastname: Israeli
authors.forename: David
authors.initials: D
authors.lastname: Tanne
authors.forename: Dianne
authors.initials: D
authors.lastname: Daniels
authors.forename: David
authors.initials: D
authors.lastname: Last
authors.forename: Ran
authors.initials: R
authors.lastname: Shneor
authors.forename: David
authors.initials: D
authors.lastname: Guez
authors.forename: Efrat
authors.initials: E
authors.lastname: Landau
authors.forename: Yiftach
authors.initials: Y
authors.lastname: Roth
authors.forename: Aharon
authors.initials: A
authors.lastname: Ocherashvilli
authors.forename: Mati
authors.initials: M
authors.lastname: Bakon
authors.forename: Chen
authors.initials: C
authors.lastname: Hoffman
authors.forename: Amit
authors.initials: A
authors.lastname: Weinberg
authors.forename: Talila
authors.initials: T
authors.lastname: Volk
authors.forename: Yael
authors.initials: Y
authors.lastname: Mardor
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001812:Blood-Brain Barrier / Q000503:physiopathology*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000556:secondary; D003287:Contrast Media / Q000493:pharmacokinetics*; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000503:physiopathology; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D020521:Stroke / Q000175:diagnosis* / Q000473:pathology; D013382:Subtraction Technique; D013997:Time Factors
chemical_list: D003287:Contrast Media