title: Assessment of oxidative stress and metabolic changes in common carp (Cyprinus carpio) acutely exposed to different concentrations of the fungicide tebuconazole.
abstract: The aim of this research is to determine the lethal concentration (LC(50-96 h)) of fungicide tebuconazole for common carp (Cyprinus carpio) and to investigate the occurrence of oxidative stress and metabolic changes after acute exposure to different concentrations of tebuconazole. For this purpose, several parameters were assessed as whole-body levels of thiobarbituric acid-reactive substance (TBARS), protein carbonyl, catalase, superoxide dismutase, glutathione S-transferase, nonprotein thiols, ascorbic acid, glycogen, glucose, lactate, protein, amino acids and ammonia in tebuconazole-exposed fish. The calculated LC(50-96 h) was 2.37 mg L(-1). Fish exhibited significant increase in TBARS levels in all concentrations used while the enzymatic and nonenzymatic antioxidants were decreased. Among the metabolic parameters, glycogen and glucose increased at 1.19 mg L(-1) and protein levels decreased at 1.78 and 2.37 mg L(-1). In conclusion, the fish health was adversely affected by exposure to tebuconazole, and those changes can compromise animal survival in the natural environment. The results indicate that the some of the parameters measured like a possible biomarkers of exposure to tebuconazole for this species of fish.
authors.affiliation: Laboratório de Bioquímica Toxicológica e Adaptativa de Peixes, Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brazil.
authors.forename: Cândida
authors.initials: C
authors.lastname: Toni
authors.forename: Daiane
authors.initials: D
authors.lastname: Ferreira
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Kreutz
authors.forename: Vania Lucia
authors.initials: VL
authors.lastname: Loro
authors.forename: Leonardo José Gil
authors.initials: LJ
authors.lastname: Barcellos
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014230:Triazoles / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D005659:Fungicides, Industrial; D014230:Triazoles; D014874:Water Pollutants, Chemical; C087114:tebuconazole; D002374:Catalase; D013482:Superoxide Dismutase