title: Systemic photochemotherapy decreases the expression of IFN-γ, IL-12p40 and IL-23p19 in psoriatic plaques.
abstract: Psoriasis vulgaris (PV) is a chronic skin disease with unclear pathogenesis. In the present study we investigated the effect of systemic photochemotherapy (PUVA therapy- psoralen and UVA therapy) on the expression of IFN-γ, IL-12p40 and IL-23p19 in lesional psoriatic skin. Fifteen patients with chronic plaque type psoriasis selected to be treated with PUVA therapy were recruited for this study. Expression of IFN-γ, IL-12p40 and IL-23p19 in psoriatic lesions before and after twenty PUVA treatments was established by using immunohistochemistry (IHC). A significant decrease in expression (p < 0.05) of IFN-γ, IL-12p40 and IL-23p19 in epidermis and dermis of psoriatic lesions was observed. The immunosuppressive effect of PUVA therapy presented with decreased expression of biologically active IL-23 (IL-12/IL-23p40 + IL-23p19) as a part of the Th17 pathway, and IFN-γ (Th1 pathway) led, in our patients, to a marked clinical improvement as shown by PASI (before therapy 20.55 and after therapy 3.33).
authors.affiliation: Department of Dermatology, University of Kragujevac, Kragujevac, Serbia. ana.ravic@sbb.rs
authors.forename: Ana
authors.initials: A
authors.lastname: Ravić-Nikolić
authors.forename: Gordana
authors.initials: G
authors.lastname: Radosavljević
authors.forename: Ivan
authors.initials: I
authors.lastname: Jovanović
authors.forename: Nemanja
authors.initials: N
authors.lastname: Zdravković
authors.forename: Slobodanka
authors.initials: S
authors.lastname: Mitrović
authors.forename: Sladjana
authors.initials: S
authors.lastname: Pavlović
authors.forename: Nebojša
authors.initials: N
authors.lastname: Arsenijević
mesh_terms: D000328:Adult; D000368:Aged; D020405:Dermis / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000378:metabolism*; D053762:Interleukin-12 Subunit p40 / Q000378:metabolism*; D053760:Interleukin-23 Subunit p19 / Q000378:metabolism*; D008875:Middle Aged; D011701:PUVA Therapy; D011565:Psoriasis / Q000188:drug therapy* / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053762:Interleukin-12 Subunit p40; D053760:Interleukin-23 Subunit p19; D007371:Interferon-gamma