title: Comparative and functional analysis of testis-specific genes.
abstract: The testis is the special male gonad responsible for spermatogenesis and steroidogenesis with complex gene expressions. Characterizing and comparing the testis-specific genes in different species can reveal key genes related to testis specific functions and provide supplementary information for study of human testis function. We screened testis-specific genes from Unigene libraries, total 317, 449 and 147 testis-specific genes were identified for human, mouse and rat, respectively. Ten from thirteen selected human testis-specific genes were validated exclusively expressed in the testis by reverse transcription polymerase chain reaction (RT-PCR). Systematic bioinformatics analysis showed that specific genes were mainly related to spermatogenesis and testis development process with significant Glycolysis and Pyruvate metabolism. Enrichment functions were discussed.
authors.affiliation: Shandong Research Centre for Stem Cell Engineering, Yu-Huang-Ding Hospital and Yan-Tai University, Yantai, Shandong Province 264000, PR China.
authors.forename: FuJun
authors.initials: F
authors.lastname: Liu
authors.forename: ShaoHua
authors.initials: S
authors.lastname: Jin
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: HaiYan
authors.initials: H
authors.lastname: Wang
authors.forename: JianYuan
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008297:Male; D051379:Mice; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*