title: The subcellular localization of a C-terminal processing protease in Pseudomonas aeruginosa.
abstract: Carboxy (C)-terminal processing proteases (CTP) are a relatively new group of serine proteases. Found in a broad range of organisms - bacteria, archaea, algae, plants and animals - these proteases are involved in the C-terminal processing of proteins. In comparison with amino-terminal processing of bacterial proteins, less is known about C-terminal processing and its physiological function. Bacterial CTPs appear to influence different basal cellular processes. Although CTPs of Gram-negative bacteria are generally referred to as being localized in the periplasm, there is little experimental evidence for this. We show for the first time the subcellular localization of a CTP-3 family protein from Pseudomonas aeruginosa, named CtpA, in the periplasm by a carefully designed fractionation study. Our results provide experimental evidence for the generally accepted hypothesis that CTPs are located in the periplasmic space of Gram-negative bacteria.
authors.affiliation: Research Centre Juelich, Institute for Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Juelich, Germany. h.l.hoge@dz.nl
authors.forename: Rien
authors.initials: R
authors.lastname: Hoge
authors.forename: Marko
authors.initials: M
authors.lastname: Laschinski
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
authors.forename: Susanne
authors.initials: S
authors.lastname: Wilhelm
authors.forename: Frank
authors.initials: F
authors.lastname: Rosenau
mesh_terms: D020418:Algal Proteins; D000595:Amino Acid Sequence; D015153:Blotting, Western; D002268:Carboxypeptidases / Q000032:analysis*; D008969:Molecular Sequence Data; D033903:Periplasmic Proteins / Q000032:analysis*; D043484:Proprotein Convertases / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D020418:Algal Proteins; D033903:Periplasmic Proteins; D002268:Carboxypeptidases; D043484:Proprotein Convertases; C073491:photosystem II, D1 polypeptide carboxy-terminal processing protease