title: Rice phospholipase Dα is involved in salt tolerance by the mediation of H(+)-ATPase activity and transcription.
abstract: Phospholipase Dα (PLDα) is involved in plant response to salt stress, but the mechanisms remain unclear. We investigated rice PLDα (OsPLDα) localization and its effect on tonoplast (TP) and plasma membrane (PM) H(+)-ATPase activity and transcription in response to NaCl. When rice suspension-cultured cells were treated with 100 mM NaCl, PLDα activity in cell extracts showed a transient activation with a threefold increase at 1 h. The amount of OsPLDα protein decreased slightly in the cytosolic fractions, whereas it increased significantly in the TP after NaCl treatment. OsPLDα1 knockdown cells were developed using RNA interference (RNAi) methods. The increase in TP and PM H(+)-ATPase activity induced by NaCl was significantly inhibited in OsPLDα1-RNAi cells. Knockdown of OsPLDα1 prevented the NaCl-induced increase in the transcript level of OsVHA-A (encodes TP H(+)-ATPase) and OSA2 (encodes PM H(+)-ATPase), as well as OsNHX1 (encodes TP Na(+) /H(+) antiporter). The cells died more in OsPLDα1-RNAi mutant than in wild type when they were treated with NaCl. These results suggest that OsPLDα is involved in salt tolerance in rice through the mediation of H(+)-ATPase activity and transcription.
authors.affiliation: College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shen
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Jing
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055785:Gene Knockdown Techniques; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013535:Suspensions; D014158:Transcription, Genetic / Q000187:drug effects; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D010739:Phospholipase D; D011494:Protein Kinases; D006180:Proton-Translocating ATPases; D012965:Sodium Chloride; D017923:Sodium-Hydrogen Exchangers; D013535:Suspensions; C454865:calcium-dependent protein kinase, rice; D017871:Calcium-Calmodulin-Dependent Protein Kinases