title: [Study of CPG15 expression in the visual cortex of normal and monocular form deprived development rats].
abstract: OBJECTIVE
  To explore the expression of CPG15 mRNA and protein the visual cortex of normal and monocular form deprived development rats, To explore the association CPG15 with visual development.
  
  
  METHODS
  It was an experimental research. one hundred healthy Wistar rats were divided into 10 groups: the time-point for normal group is postnatal 7, 14, 21, 28, 45 days. The time-point for MD group is postnatal 21, 28, 45 days, 10 in every group. MD groups were sutured right eyelid at postnatal 14 days, and to make the model of monocular form deprived. Rats were housed in alike environment. To detect the expression of Neuritin mRNA and immuno-positive neurons protein in the visual cortex of normal and monocular form deprived rats by SYBR green I real-time fluorescent polymerase chain reaction and immunohistochemistry stain methods.
  
  
  RESULTS
  We found that: (1) The expression of normal and MD rats visual cortex CPG15 mRNA and immuno-positive neurons and the IOD of protein, significant deviation existed among different ages with one-factor analysis of variance (P < 0.05), and reached its climax in P28 and MD28. (2) The expression of CPG15 mRNA and immuno-positive neurons and the IOD of protein in MD group obviously lower than the normal group in postnatal 21, 28, 45 days, there was statistically different in that ages (P < 0.05).
  
  
  CONCLUSIONS
  The expression of CPG15 mRNA and immuno-positive neurons and the IOD of protein in the development normal and MD rats show that the stage change by development vision. Its expression correlated with visual experience and age factor, and there was a high expression in critical period, and obviously influence by monocular form deprived. So we supposed that CPG15 may affect development and maturation in the visual cortical neuron. It may be the one of molecular basis to participated in visual developmental plasticity.
authors.affiliation: Tianjin Eye Hospital, Tianjin 300020, China. chenxia9453@yahoo.com.cn
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Chun-hui
authors.initials: CH
authors.lastname: Tian
authors.forename: Jin-yong
authors.initials: JY
authors.lastname: Lin
authors.forename: Yu-chuan
authors.initials: YC
authors.lastname: Wang
mesh_terms: D000818:Animals; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012683:Sensory Deprivation; D013285:Strabismus / Q000378:metabolism*; D015349:Vision, Monocular; D014793:Visual Cortex / Q000254:growth & development / Q000378:metabolism*
chemical_list: C114622:Cpg15 protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins