title: [Expression of brain-derived neurotrophic factor modified bone marrow mesenchymal stem cells in the cochlea of drug deafened guinea pigs and its protection role].
abstract: OBJECTIVE
  To study the expression of brain-derived neurotrophic factor (BDNF) gene modified bone marrow mesenchymal stem cells (MSC) in the cochlea of drug-deafened guinea pigs and its protection to spiral ganglion cells (SGC).
  
  
  METHODS
  Guinea pigs deafened by subcutaneous injection of amikacin were randomly divided into two groups, BDNF gene modified bone marrow MSC were injected into the cochlea through fenestration of scala tympani in the experimental group, while artificial perilymphatic fluid were injected in the control group. Experimental animals were executed at 7 and 28 days post-operation. Expression of BDNF mRNA was examined by quantitate real time RT-PCR, histological images of cochlear sections were analyzed to calculate the cellular density of the SGC, and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) was used to identify the apoptotic neurons.
  
  
  RESULTS
  The BDNF expressive level in experimental group was higher than in the control group at 7 d and 28 d post-operation, whose differences were both statistically significant (P < 0.01). And, It showed a higher abundance of ganglion cell numbers, as well as a decreased apoptotic index in experimental group compared with the control group at 7 d and 28 d post-operation, whose differences were all statistically significant (P < 0.01).
  
  
  CONCLUSIONS
  BDNF gene modified MSC could maintain expression for at least 28 days after transplantation into cochlea of drug deafened guinea pigs, and protect SGC.
authors.affiliation: Department of Otorhinolaryngology, Second Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Guan-gui
authors.initials: GG
authors.lastname: Chen
authors.forename: Ding-hua
authors.initials: DH
authors.lastname: Xie
authors.forename: Qian-xu
authors.initials: QX
authors.lastname: Liu
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Tan
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000494:pharmacology; D003638:Deafness / Q000139:chemically induced / Q000628:therapy*; D006168:Guinea Pigs; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D013136:Spiral Ganglion / Q000187:drug effects*
chemical_list: D019208:Brain-Derived Neurotrophic Factor