title: Electron ionization in LC-MS: recent developments and applications of the direct-EI LC-MS interface.
abstract: The purpose of this article is to underline the possibility of efficiently using electron ionization (EI) in liquid chromatography (LC) and mass spectrometry (MS). From a historical perspective, EI accompanied the first attempts in LC-MS but, owing to several technical shortcomings, it was soon outshined by soft, atmospheric pressure ionization (API) techniques. Nowadays, two modern approaches, supersonic molecular beam LC-MS and direct-EI LC-MS, offer a valid alterative to API, and preserve the advantages of EI also in LC-MS applications. These advantages can be summarized in three crucial aspects: automated library identification; identification of unknown compounds, owing to EI extensive fragment information; inertness to coeluted matrix interferences owing to very unlikely ion-ion and ion-molecule interactions in the EI gas-phase environment. The direct-EI LC-MS interface is a simple and efficient solution able to produce high-quality, interpretable EI spectra from a wide range of low molecular weight molecules of different polarity. Because of the low operative flow rates, this interface relies on a nano-LC technology that helps in reducing the impact of the mobile phase on the gas-phase environment of EI. This review provides an extensive discussion on the role of EI in LC-MS interfacing, and presents in detail several performance aspects of the direct-EI LC-MS interface, especially in terms of response, mass-spectral quality, and matrix effects. In addition, several key applications are also reported.
authors.affiliation: Dipartimento GeoTeCA, Laboratorio LC-MS, Università di Urbino, Italy.
authors.forename: Pierangela
authors.initials: P
authors.lastname: Palma
authors.forename: Giorgio
authors.initials: G
authors.lastname: Famiglini
authors.forename: Helga
authors.initials: H
authors.lastname: Trufelli
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Pierini
authors.forename: Veronica
authors.initials: V
authors.lastname: Termopoli
authors.forename: Achille
authors.initials: A
authors.lastname: Cappiello