title: Using solid-state ¹³C NMR spectroscopy to study the molecular organisation of primary plant cell walls.
abstract: Studies of the mobilities of polysaccharides or parts of polysaccharides in a cell-wall preparation may give clues about the molecular interactions among the polysaccharides in the cell wall and the relative locations of polysaccharides within the cell wall. A number of solid-state (13)C NMR techniques have been developed that can be used to investigate different types of polysaccharide mobilities: rigid, semi-rigid, mobile, and highly mobile. In this chapter, techniques are described for obtaining spectra from primary cell-wall preparations using CP/MAS, proton-rotating frame, proton spin-spin, spin-echo relaxation spectra, and single-pulse excitation. We also describe how proton spin relaxation editing can be used to obtain subspectra for cell-wall polysaccharides of different mobilities.
authors.affiliation: School of Biological Sciences, The University of Auckland, Auckland, New Zealand.
authors.forename: Tracey J
authors.initials: TJ
authors.lastname: Bootten
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Harris
authors.forename: Laurence D
authors.initials: LD
authors.lastname: Melton
authors.forename: Roger H
authors.initials: RH
authors.lastname: Newman
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry
chemical_list: D011134:Polysaccharides