title: Platelet concentrates from fresh or overnight-stored blood, an international study.
abstract: BACKGROUND
  Whole blood and also buffy coats (BCs) can be held for a few hours or overnight before processing into blood components or platelet concentrates (PCs). Individual studies have reported a range of outcomes regarding in vitro variables for PCs prepared from fresh and stored whole blood. In this multicenter study, effects of storage of whole blood or BCs on the in vitro quality of PCs were studied.
  
  
  METHODS
  The leukoreduced BC PCs were prepared from fresh BCs (2-8 hr after collection; fresh/fresh), from BCs at 20 to 24 hours after collection (fresh/stored), or from BCs prepared from whole blood stored for 20 to 24 hours (stored/fresh). PCs were stored on a flat-bed shaker at 20 to 24°C for 7 days. PCs were tested on Days 0 (only fresh/fresh), 1, 5, and 7 for in vitro quality. There were six participating centers that tested all three conditions with n = 6 per condition.
  
  
  RESULTS
  In comparison to fresh/stored and stored/fresh PCs, fresh/fresh PCs exhibited a lower platelet (PLT) count (Day 1-220 × 10(9) ± 70 × 10(9) vs. 324 × 10(9) ± 50 × 10(9) and 368 × 10(9) ± 56 × 10(9) PLTs/PC), lactate, pCO(2), and hypotonic shock response (HSR; Days 5 and 7; Day 7-50 ± 13% vs. 57 ± 12 and 63 ± 11%) and a higher pH, glucose, pO(2), and CD62P expression (than stored/fresh PCs only; Day 7-33 ± 10% vs. 28 ± 12 and 24 ± 11%; p < 0.05). No differences were observed for volume, swirling effect, white blood cell count, annexin V binding, or aggregation between these conditions.
  
  
  CONCLUSIONS
  Based on PLT count, HSR, and PLT activation, PCs are best prepared after 20 to 24 hours hold of the whole blood or BCs.
authors.affiliation: Sanquin Blood Bank North East Region, Groningen, the Netherlands. m.dijkstra@sanquin.nl
authors.forename: M J
authors.initials: MJ
authors.lastname: Dijkstra-Tiekstra
authors.forename: P F
authors.initials: PF
authors.lastname: van der Meer
authors.forename: R
authors.initials: R
authors.lastname: Cardigan
authors.forename: D
authors.initials: D
authors.lastname: Devine
authors.forename: C
authors.initials: C
authors.lastname: Prowse
authors.forename: P
authors.initials: P
authors.lastname: Sandgren
authors.forename: J
authors.initials: J
authors.lastname: de Wildt-Eggen
mesh_terms: D001781:Blood Component Removal / Q000379:methods*; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D001793:Blood Preservation / Q000379:methods*; D048429:Cell Size; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047589:Leukocyte Reduction Procedures; D009996:Osmotic Fragility; D010100:Oxygen / Q000097:blood; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation; D010976:Platelet Count; D013696:Temperature; D013997:Time Factors
chemical_list: D019007:P-Selectin; D010100:Oxygen