title: Crystallization and preliminary X-ray crystallographic analysis of the short-chain dehydrogenase/reductase-type DDB_G0291732 protein from Dictyostelium discoideum.
abstract: The DDB_G0291732 gene product from Dictyostelium discoideum, which is a NmrA-like protein that belongs to the short-chain dehydrogenase/reductase superfamily but shows deviations in conserved sequence regions, has been crystallized by the hanging-drop vapour-diffusion method at 295 K. A 1.65 Å resolution data set was collected using synchrotron radiation. The crystals of DDB_G0291732 protein belonged to space group P2(1), with unit-cell parameters a=38.5, b=63.7, c=56.0 Å, β=91.7°. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 38.1%.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Hyung Soon
authors.initials: HS
authors.lastname: Yim
authors.forename: Sa Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004023:Dictyostelium / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D015800:Protozoan Proteins