title: Scavenger receptor WC1 contributes to the γδ T cell response to Leptospira.
abstract: WC1 molecules are exclusively expressed on the surface of γδ T cells. They belong to the scavenger receptor cysteine-rich (SRCR) superfamily and are encoded by a multi-gene family. WC1 molecules have been grouped on the basis of antibody reactivity. The expression of WC1 molecules from these serologically defined groups is correlated with differences in γδ T cell responses. The expression of receptors within the WC1.1 group correlates with the capacity of γδ T cells to respond to Leptospira antigen. In this study, we used RNA interference to directly investigate the role of WC1 expression in the response to Leptospira borgpetersenii. We found that when three out of thirteen WC1 gene products were downregulated by RNA interference, γδ T cell proliferation and IFN-γ production in response to Leptospira antigen was significantly reduced. Our data demonstrate that specific receptors in the WC1 family directly participate in Leptospira recognition and/or activation of γδ T cells.
authors.affiliation: Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, United States.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Carolyn T A
authors.initials: CT
authors.lastname: Herzig
authors.forename: Chuang
authors.initials: C
authors.lastname: Chen
authors.forename: Haoting
authors.initials: H
authors.lastname: Hsu
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Baldwin
authors.forename: Janice C
authors.initials: JC
authors.lastname: Telfer
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D049109:Cell Proliferation; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007919:Leptospira / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D051116:Receptors, Scavenger / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*
chemical_list: C086500:DMBT1 protein, Bos taurus; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; D016692:Receptors, Antigen, T-Cell, gamma-delta; D051116:Receptors, Scavenger; D007371:Interferon-gamma