title: [Effect of tamoxifen on proliferation of cultured breast cancer and cervical carcinoma cell lines].
abstract: OBJECTIVE
  To investigate the effects of tamoxifen on proliferation of human breast cancer Bcap-37 cells and cervical carcinoma HeLa cells and to explore it's possible mechanism.
  
  
  METHODS
  The techniques of cell culture, growth curves, flow cytometry and laser scanning confocal microscope were used.
  
  
  RESULTS
  Tamoxifen (10(-6) mol/L) shifted the growth curve of Bcap-37 cells downward, and shifted the growth curve of HeLa cells upward. Tamoxifen (10(-8) - 10(-6) mol/L) inhibited the proliferation of Bcap-37 cells in a dose-dependent manner, but stimulated the proliferation of HeLa cells in a dose-dependent manner. Bcap-37 cells appeared apoptosis when treated with tamoxifen (10(-6) mol/L), and the same dose stimulated the proliferation of HeLa cells at GI/S phases. The apoptotic rate of Bcap-37 cells was 97.5%. It blocked G1 phase of HeLa cells from 55.5% to 32.8%, and increased the S phase from 29.0% to 49.4%. Tamoxifen (10(-6) mol/L) also increased the releasing of calcium in Bcap-37 and HeLa cells.
  
  
  CONCLUSIONS
  Tamoxifen can significantly influence the proliferation of breast cancer and cervical carcinoma cells possibly by affecting cell cycle and stimulating the releasing of Ca2+ in the cells.
authors.affiliation: Department of Physiology, The Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Zi-ying
authors.initials: ZY
authors.lastname: Zou
authors.forename: Yun-long
authors.initials: YL
authors.lastname: Zhu
authors.forename: Gao-feng
authors.initials: GF
authors.lastname: Wang
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Zhong
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D013629:Tamoxifen / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology*
chemical_list: D013629:Tamoxifen