title: Changes in glucose and glutamine lymphocyte metabolisms induced by type I interferon α.
abstract: In lymphocytes (LY), the well-documented antiproliferative effects of IFN-α are associated with inhibition of protein synthesis, decreased amino acid incorporation, and cell cycle arrest. However, the effects of this cytokine on the metabolism of glucose and glutamine in these cells have not been well investigated. Thus, mesenteric and spleen LY of male Wistar rats were cultured in the presence or absence of IFN-α, and the changes on glucose and glutamine metabolisms were investigated. The reduced proliferation of mesenteric LY was accompanied by a reduction in glucose total consumption (35%), aerobic glucose metabolism (55%), maximal activity of glucose-6-phosphate dehydrogenase (49%), citrate synthase activity (34%), total glutamine consumption (30%), aerobic glutamine consumption (20.3%) and glutaminase activity (56%). In LY isolated from spleen, IFNα also reduced the proliferation and impaired metabolism. These data demonstrate that in LY, the antiproliferative effects of IFNα are associated with a reduction in glucose and glutamine metabolisms.
authors.affiliation: Department of Physical Education, Federal University of Maranhão, 14040-904 São Paulo, SP, Brazil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Navarro
authors.forename: Aline V N
authors.initials: AV
authors.lastname: Bacurau
authors.forename: Andréa
authors.initials: A
authors.lastname: Vanzelli
authors.forename: Marcela
authors.initials: M
authors.lastname: Meneguello-Coutinho
authors.forename: Marco C
authors.initials: MC
authors.lastname: Uchida
authors.forename: Milton R
authors.initials: MR
authors.lastname: Moraes
authors.forename: Sandro S
authors.initials: SS
authors.lastname: Almeida
authors.forename: Frederick
authors.initials: F
authors.lastname: Wasinski
authors.forename: Carlos C
authors.initials: CC
authors.lastname: Barros
authors.forename: Martin
authors.initials: M
authors.lastname: Würtele
authors.forename: Ronaldo C
authors.initials: RC
authors.lastname: Araújo
authors.forename: Luís F B
authors.initials: LF
authors.lastname: Costa Rosa
authors.forename: Reury F P
authors.initials: RF
authors.lastname: Bacurau
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D016898:Interferon-alpha / Q000276:immunology / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008643:Mesentery / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology
chemical_list: D007155:Immunologic Factors; D016898:Interferon-alpha; D005973:Glutamine; D005947:Glucose