title: Mechanistic characterization of sulfur transfer from cysteine desulfurase SufS to the iron-sulfur scaffold SufU in Bacillus subtilis.
abstract: Iron-sulfur cluster biosynthesis in Gram-positive bacteria is mediated by the SUF system. The transfer of sulfide from the cysteine desulfurase SufS to the scaffold protein SufU is one of the first steps within the assembly process. In this study, we analyzed the interaction between Bacillus subtilis SufS and its scaffold SufU. The activity of SufS represents a Ping-Pong mechanism leading to successive sulfur loading of the conserved cysteine residues in SufU. Cysteine 41 of SufU is shown to be essential for receiving sulfide from SufS, while cysteines 66 and 128 are needed for SufS/SufU interaction. In conclusion, we present the first step-by-step model for loading of the essential scaffold component SufU by its sulfur donor SufS.
authors.affiliation: Fachbereich Chemie/Biochemie der Philipps Universität Marburg, Marburg, Germany.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Albrecht
authors.forename: Florian
authors.initials: F
authors.lastname: Peuckert
authors.forename: Hannes
authors.initials: H
authors.lastname: Landmann
authors.forename: Marcus
authors.initials: M
authors.lastname: Miethke
authors.forename: Andreas
authors.initials: A
authors.lastname: Seubert
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Marahiel
mesh_terms: D019943:Amino Acid Substitution; D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D055162:Biocatalysis; D013437:Carbon-Sulfur Lyases / Q000737:chemistry* / Q000378:metabolism*; D020134:Catalytic Domain; D003545:Cysteine / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000201:enzymology / Q000378:metabolism; D007501:Iron / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins; D013455:Sulfur / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; D013455:Sulfur; D007501:Iron; D013437:Carbon-Sulfur Lyases; C080546:cysteine desulfurase; D003545:Cysteine