title: Optimization of α-Terpineol production by the biotransformation of R-(+)-limonene and (-)-β-pinene.
abstract: The main objective of this work is to present the optimization of the biotransformation of R-(+)-limonene and (-)-β-pinene aiming at the production of α-terpineol by strains of fungal and yeasts previously isolated by our research group using the methodology of experimental design. New optimized experimental data on α-terpineol production by the biotransformation of R-(+)-limonene and (-)-β-pinene using newly isolated microorganisms are reported in this work. Conversion of about 1,700 mg/L was achieved when R-(+)-limonene was used as substrate and the newly isolated strain 05.01.35 as microorganism at the central point of the experimental design, corresponding to a substrate concentration of 1.75%, mass of inoculum of 2 g, and substrate to ethanol volume ratio of 1:1. The same experimental condition led to higher conversions when (-)-β-pinene was used as substrates and the strains coded as 04.05.08 and 01.04.03 as microorganism. Here, conversions of about 770 mg/L were achieved.
authors.affiliation: Department of Biochemistry, Instituto de Química-UFRJ, CT, Rio de Janeiro, Brazil.
authors.forename: Ieda
authors.initials: I
authors.lastname: Rottava
authors.forename: Priscila F
authors.initials: PF
authors.lastname: Cortina
authors.forename: Eduarda
authors.initials: E
authors.lastname: Martello
authors.forename: Rogerio L
authors.initials: RL
authors.lastname: Cansian
authors.forename: Geciane
authors.initials: G
authors.lastname: Toniazzo
authors.forename: Octavio A C
authors.initials: OA
authors.lastname: Antunes
authors.forename: Enrique G
authors.initials: EG
authors.lastname: Oestreicher
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: Debora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D001230:Aspergillus / Q000378:metabolism; D000081008:Bicyclic Monoterpenes; D001673:Biodegradation, Environmental; D001643:Bridged Bicyclo Compounds / Q000737:chemistry* / Q000378:metabolism; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry* / Q000378:metabolism*; D000077222:Limonene; D039821:Monoterpenes / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism; D013729:Terpenes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D013729:Terpenes; C016775:alpha-terpineol; C010789:beta-pinene; D000077222:Limonene