title: Protective effects of plasma alpha-tocopherols on the risk of inorganic arsenic-related urothelial carcinoma.
abstract: Arsenic plays an important role in producing oxidative stress in cultured cells. To investigate the interaction between high oxidative stress and low arsenic methylation capacity on arsenic carcinogenesis, a case-control study was conducted to evaluate the relationship among the indices of oxidative stress, such as urinary 8-hydroxydeoxyquanine (8-OHdG), as well as plasma micronutrients and urinary arsenic profiles on urothelial carcinoma (UC) risk. Urinary 8-OHdG was measured using high-sensitivity enzyme-linked immunosorbent assay kits. The urinary arsenic species were analyzed using high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Plasma micronutrient levels were analyzed using reversed-phase high-performance liquid chromatography. The present study showed a significant protective effect of plasma alpha-tocopherol on UC risk. Plasma alpha-tocopherol levels were significantly inversely related to urinary total arsenic concentrations and inorganic arsenic percentage (InAs%), and significantly positively related to dimethylarsinic acid percentage (DMA%). There were no correlations between plasma micronutrients and urinary 8-OHdG. Study participants with lower alpha-tocopherol and higher urinary total arsenic, higher InAs%, higher MMA%, and lower DMA% had a higher UC risk than those with higher alpha-tocopherol and lower urinary total arsenic, lower InAs%, lower MMA%, and higher DMA%. These results suggest that plasma alpha-tocopherol might modify the risk of inorganic arsenic-related UC.
authors.affiliation: School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan.
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Chung
authors.forename: Yeong-Shiau
authors.initials: YS
authors.lastname: Pu
authors.forename: Ying-Ting
authors.initials: YT
authors.lastname: Chen
authors.forename: Chien-Tien
authors.initials: CT
authors.lastname: Su
authors.forename: Chia-Chang
authors.initials: CC
authors.lastname: Wu
authors.forename: Horng-Sheng
authors.initials: HS
authors.lastname: Shiue
authors.forename: Chao-Yuan
authors.initials: CY
authors.lastname: Huang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Hsueh
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000975:Antioxidants / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity* / Q000652:urine; D002274:Carcinogens, Environmental / Q000378:metabolism / Q000633:toxicity*; D002277:Carcinoma / Q000453:epidemiology / Q000378:metabolism / Q000517:prevention & control*; D016022:Case-Control Studies; D004249:DNA Damage; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism / Q000652:urine; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000187:drug effects; D012307:Risk Factors; D014571:Urologic Neoplasms / Q000453:epidemiology / Q000378:metabolism / Q000517:prevention & control*; D024502:alpha-Tocopherol / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D002274:Carcinogens, Environmental; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D024502:alpha-Tocopherol; D001151:Arsenic