title: The HIV-1 integrase α4-helix involved in LTR-DNA recognition is also a highly antigenic peptide element.
abstract: Monoclonal antibodies (MAbas) constitute remarkable tools to analyze the relationship between the structure and the function of a protein. By immunizing a mouse with a 29mer peptide (K159) formed by residues 147 to 175 of the HIV-1 integrase (IN), we obtained a monoclonal antibody (MAba4) recognizing an epitope lying in the N-terminal portion of K159 (residues 147-166 of IN). The boundaries of the epitope were determined in ELISA assays using peptide truncation and amino acid substitutions. The epitope in K159 or as a free peptide (pep-a4) was mostly a random coil in solution, while in the CCD (catalytic core domain) crystal, the homologous segment displayed an amphipathic helix structure (α4-helix) at the protein surface. Despite this conformational difference, a strong antigenic crossreactivity was observed between pep-a4 and the protein segment, as well as K156, a stabilized analogue of pep-a4 constrained into helix by seven helicogenic mutations, most of them involving hydrophobic residues. We concluded that the epitope is freely accessible to the antibody inside the protein and that its recognition by the antibody is not influenced by the conformation of its backbone and the chemistry of amino acids submitted to helicogenic mutations. In contrast, the AA →Glu mutations of the hydrophilic residues Gln148, Lys156 and Lys159, known for their interactions with LTRs (long terminal repeats) and inhibitors (5CITEP, for instance), significantly impaired the binding of K156 to the antibody. Moreover, we found that in competition ELISAs, the processed and unprocessed LTR oligonucleotides interfered with the binding of MAba4 to IN and K156, confirming that the IN α4-helix uses common residues to interact with the DNA target and the MAba4 antibody. This also explains why, in our standard in vitro concerted integration assays, MAba4 strongly impaired the IN enzymatic activity.
authors.affiliation: LBPA, ENS de Cachan, CNRS, Cachan, France.
authors.forename: Sandy
authors.initials: S
authors.lastname: Azzi
authors.forename: Vincent
authors.initials: V
authors.lastname: Parissi
authors.forename: Richard G
authors.initials: RG
authors.lastname: Maroun
authors.forename: Pierre
authors.initials: P
authors.lastname: Eid
authors.forename: Olivier
authors.initials: O
authors.lastname: Mauffret
authors.forename: Serge
authors.initials: S
authors.lastname: Fermandjian
mesh_terms: D000906:Antibodies / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000941:Antigens / Q000737:chemistry; D002384:Catalysis; D004247:DNA / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry; D019427:HIV Integrase / Q000737:chemistry* / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D009154:Mutation; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D020079:Terminal Repeat Sequences / Q000187:drug effects*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D010455:Peptides; D004247:DNA; D019427:HIV Integrase