title: [Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants].
abstract: OBJECTIVE
  To investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum.
  
  
  METHODS
  We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation.
  
  
  RESULTS
  (1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity.
  
  
  CONCLUSIONS
  17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.
authors.affiliation: Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Cha-Xiang
authors.initials: CX
authors.lastname: Guan
authors.forename: Fu-Wen
authors.initials: FW
authors.lastname: Zhou
authors.forename: Zi-Qiang
authors.initials: ZQ
authors.lastname: Luo
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Xiao-Qun
authors.initials: XQ
authors.lastname: Qin
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D004958:Estradiol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002147:Calmodulin; D016895:Culture Media, Serum-Free; D004958:Estradiol; D011493:Protein Kinase C; D019916:Choline-Phosphate Cytidylyltransferase