title: Effects of microtubule modulators on HIV-1 infection of transformed and resting CD4 T cells.
abstract: Previous studies have observed fluorescently labeled HIV particles tracking along microtubule networks for nuclear localization. To provide direct evidence for the involvement of microtubules in early steps of HIV infection of human CD4 T cells, we used multiple microtubule modulators such as paclitaxel (originally called taxol; 1 μM), vinblastine (1 and 10 μM), colchicine (10 and 100 μM), and nocodazole (10 and 100 μM) to disturb microtubule networks in transformed and resting CD4 T cells. Although these drugs disrupted microtubule integrity, almost no inhibition of HIV-1 infection was observed. Our results do not appear to support an essential role for microtubules in the initiation of HIV infection of CD4 T cells.
authors.affiliation: Department of Molecular and Microbiology, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA.
authors.forename: Alyson
authors.initials: A
authors.lastname: Yoder
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.forename: Dongyang
authors.initials: D
authors.lastname: Yu
authors.forename: Zongqiang
authors.initials: Z
authors.lastname: Cui
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Wu
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000187:drug effects* / Q000378:metabolism*; D050257:Tubulin Modulators / Q000494:pharmacology*; D053586:Virus Internalization
chemical_list: D050257:Tubulin Modulators