title: Volume loading reduces pulmonary vascular resistance in ventilated animals with acute lung injury: evaluation of RV afterload.
abstract: During mechanical ventilation, increased pulmonary vascular resistance (PVR) may decrease right ventricular (RV) performance. We hypothesized that volume loading, by reducing PVR, and, therefore, RV afterload, can limit this effect. Deep anesthesia was induced in 16 mongrel dogs (8 oleic acid-induced acute lung injury and 8 controls). We measured ventricular pressures, dimensions, and stroke volumes during positive end-expiratory pressures of 0, 6, 12, and 18 cmH(2)O at three left ventricular (LV) end-diastolic pressures (5, 12, and 18 mmHg). Oleic acid infusion (0.07 ml/kg) increased PVR and reduced respiratory system compliance (P < 0.05). With positive end-expiratory pressure, PVR was greater at a lower LV end-diastolic pressure. Increased PVR was associated with a decreased transseptal pressure gradient, suggesting that leftward septal shift contributed to decreased LV preload, in addition to that caused by external constraint. Volume loading reduced PVR; this was associated with improved RV output and an increased transseptal pressure gradient, which suggests that rightward septal shift contributed to the increased LV preload. If PVR is used to reflect RV afterload, volume loading appeared to reduce PVR, thereby improving RV and LV performance. The improvement in cardiac output was also associated with reduced external constraint to LV filling; since calculated PVR is inversely related to cardiac output, increased LV output would reduce PVR. In conclusion, our results, which suggest that PVR is an independent determinant of cardiac performance, but is also dependent on cardiac output, improve our understanding of the hemodynamic effects of volume loading in acute lung injury.
authors.affiliation: Department of Cardiac Sciences, University of Calgary, Alberta, Canada. jrmitche@ucalgary.ca
authors.forename: Jamie R
authors.initials: JR
authors.lastname: Mitchell
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Doig
authors.forename: William A
authors.initials: WA
authors.lastname: Whitelaw
authors.forename: John V
authors.initials: JV
authors.lastname: Tyberg
authors.forename: Israel
authors.initials: I
authors.lastname: Belenkie
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D008170:Lung Compliance; D008297:Male; D008955:Models, Cardiovascular; D019301:Oleic Acid; D011175:Positive-Pressure Respiration / Q000009:adverse effects; D011652:Pulmonary Circulation; D013318:Stroke Volume; D014655:Vascular Resistance; D018497:Ventricular Dysfunction, Right / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D016277:Ventricular Function, Left; D016278:Ventricular Function, Right; D017725:Ventricular Pressure
chemical_list: D019301:Oleic Acid