title: [Effects of CNTF on the nuclear translocation of PKC following NMDA administration in primary hippocampal neurons].
abstract: OBJECTIVE
  To investigate the effect of ciliary neurotrophic factor (CNTF) on the nuclear translocation of protein kinase C (PKC) following NMDA administration in the primary cultured hippocampal neurons.
  
  
  METHODS
  (1) PKCGAMMA or PKCepsilon- immunocytochemistry staining method was used after treating neurons with NMDA or CNTF. (2) The gray of the nucleus of the PKC-positive neurons were measured under the image pattern analysis system.
  
  
  RESULTS
  (1) After NMDA administration of different concentration and time, Nucleus appear PKCgamma and PKCepsilon activities, especially the 100 micromol/L NMDA 30 min group. (2) The gray of nucleus in CNTF + 500 micromol/L NMDA group is similar to control group.
  
  
  CONCLUSIONS
  NMDA can induce nuclear translocation of PKC in the primary cultured hippocampal neurons, and CNTF can inhibit the translocation. It suggests that the inhibition of PKC translocation induced by NMDA is one of the important reasons for the neuro-protective effects of CNTF.
authors.affiliation: Department of Neurobiology, Second Medical Military University, Shanghai 200025, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.forename: Zong-lai
authors.initials: ZL
authors.lastname: Jiang
authors.forename: Xue-qi
authors.initials: XQ
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Bao
authors.forename: Chang-lin
authors.initials: CL
authors.lastname: Lu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020934:Ciliary Neurotrophic Factor; D016202:N-Methylaspartate; D011493:Protein Kinase C