title: Characterization of an Aspergillus oryzae cysteinyl dipeptidase expressed in Escherichia coli.
abstract: Cysteinyl dipeptidase from Aspergillus oryzae (CdpA) was produced in Escherichia coli and purified. The enzyme showed activity specific toward cysteine-containing dipeptides, but its substrate specificity was distinct from those of other cysteinyl dipeptidases of the M20 family. It was optimally active at pH 7-8 and stable at pH 6-9 and at up to 40 °C.
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Hattori
authors.forename: Mayumi
authors.initials: M
authors.lastname: Matsushita-Morita
authors.forename: Junichiro
authors.initials: J
authors.lastname: Marui
authors.forename: Sawaki
authors.initials: S
authors.lastname: Tada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Furukawa
authors.forename: Youhei
authors.initials: Y
authors.lastname: Yamagata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Amano
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishida
authors.forename: Michio
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kusumoto
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D003001:Cloning, Molecular; D003545:Cysteine / Q000378:metabolism*; D004150:Dipeptidases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D009842:Oligopeptides; D004150:Dipeptidases; C038937:dipeptidase; D003545:Cysteine