title: Central interleukin-10 attenuated lipopolysaccharide-induced changes in core temperature and hypothalamic glutamate, hydroxyl radicals and prostaglandin-E(2).
abstract: It has been documented that intravenous lipopolysaccharide (LPS) in rabbits causes fever accompanied by increased levels of extracellular glutamate, hydroxyl radicals, and prostaglandin E(2) (PGE(2)) in the hypothalamus and circulating tumor necrosis factor-alpha (TNF-α). This investigation was to determine whether central interleukin-10 (IL-10) exerted its antipyresis by reducing changes in circulating TNF-α and extracellular glutamate, hydroxyl radicals and PGE(2) in the hypothalamus. The microdialysis probes were stereotaxically and chronically implanted into the preoptic anterior hypothalamus of rabbit brain for determinating extracellular glutamate, hydroxyl radicals, and PGE(2) in situ. It was found that systemically injected LPS (2μg/kg, intravenously) increased the levels of core temperature, and extracellular glutamate, hydroxyl radicals, and PGE(2) in the hypothalamus accompanied by increased plasma levels of TNF-α. Pretreatment with IL-10 (10-100ng, intracerebroventricularly) 1h before intravenous LPS significantly reduced the LPS-induced changes in extracellular glutamate, hydroxyl radicals, and PGE(2) in the hypothalamus and fever, but not the increased levels of TNF-α in rabbits. These findings suggested that directly injected IL-10 into the lateral cerebral ventricle 1h before intravenous LPS exerted its antipyresis by inhibiting the changes in extracellular glutamate, hydroxyl radicals and PGE(2) in the hypothalamus during LPS fever in rabbits.
authors.affiliation: Department of Surgery, Chi Mei Medical Center, Tainan 710, Taiwan.
authors.forename: Cheng-Hsing
authors.initials: CH
authors.lastname: Kao
authors.forename: Wu-Tein
authors.initials: WT
authors.lastname: Huang
authors.forename: Mao-Tsun
authors.initials: MT
authors.lastname: Lin
authors.forename: Wen-Shiann
authors.initials: WS
authors.lastname: Wu
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D002396:Catechols / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D005334:Fever / Q000139:chemically induced; D018698:Glutamic Acid / Q000378:metabolism*; D062385:Hydroxybenzoates; D017665:Hydroxyl Radical / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D016753:Interleukin-10 / Q000008:administration & dosage* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D017551:Microdialysis; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D002396:Catechols; D062385:Hydroxybenzoates; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D017665:Hydroxyl Radical; D018698:Glutamic Acid; C009135:2,3-dihydroxybenzoic acid; D015232:Dinoprostone