title: [Mast cell and substance P are involved in the process of acupoint sensitization induced by acute gastric mucosal injury].
abstract: OBJECTIVE
  To observe changes of mast cells (MCs) number and morphology, and substance P (SP) expression in Evans blue (EB) extravasated region around acupoint "Pishu" (BL 20) and "Weishu" (BL 21) after acute gastric mucosal injury (AGMI) so as to investigate the mechanism underlying visceral problems-induced acupoint activation.
  
  
  METHODS
  Thirty adult Wistar rats were randomly divided into normal control (n = 15) and AGMI groups (n = 15). AGMI model was duplicated by perfusing the rats with 0.5 mol/L HCl (1 mL/100 g) after fasting for 20 h. Five hours after AGMI, the rats were treated by tail-intravenous injection of EB dye (5 mg/100 g, 50 mg/mL in normal saline) for inducing dye-plasma extravasation in the skin around BL 20, BL 21 regions, etc. at the back. The rats of the normal control group were treated with tail-intravenous injection of 0.9% NaCl. The skin and subcutaneous tissues (2 mmx 2 mm) of extravasated EB dye points (BL 20 or BL 21 region) and those 2 mm lateral to the extravasated EB dye points in the model group and the corresponding points in the normal control group were sampled (followed by fixing them in 4% paraformaldehyde), sectioned and stained by toluidine blue (for labeling MCs). The expression of SP in the extravasated EB dye skin and subcutaneous tissues was detected by immunohistochemistry (n = 5) and western blot (n = 5) respectively. The number of MCs in these samples was counted and the degranulation rate of MCs calculated.
  
  
  RESULTS
  The total number of MCs and the number of degranulated MCs were significantly more in the EB extravasation points (corresponding to BL 20/BL 21 area) of AGMI group than those in the control spots of AGMI group and than those in the normal control group (P < 0.05, P < 0.001). The degranulation rate of MCs was significantly higher in the EB extravasation points of AGMI group than those in the control spots of AGMI group and in the normal control group (P < 0.01). In comparison with normal control group, the SP expression level was increased consideraly in the control spots of AGMI group and AGMI group (P < 0.01).
  
  
  CONCLUSIONS
  After AGMI, the numbers of MCs and the degranulated MCs, and the SP expression level in BL 20/BL 21 area were increased significantly, suggesting an involvement of MCs and SP in the process of AGMI-induced activation of acupoints.
authors.affiliation: Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Cheng
authors.forename: Jiang-Hui
authors.initials: JH
authors.lastname: Li
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Chen
authors.forename: Qi-Wen
authors.initials: QW
authors.lastname: Tan
authors.forename: Zhi-Gao
authors.initials: ZG
authors.lastname: Jin
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Jing
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D005070:Evans Blue / Q000032:analysis; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry / Q000293:injuries / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D008407:Mast Cells / Q000737:chemistry / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D013373:Substance P / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D013373:Substance P; D005070:Evans Blue