title: Metabolomic study of a rat fever model induced with 2,4-dinitrophenol and the therapeutic effects of a crude drug derived from Coptis chinensis.
abstract: This study describes the metabonomics of fevers in animal models and the therapeutic effects of Rhizoma coptidis extract (RCE) on them. The rat urinary samples were analyzed by UPLC/ ESI-Q-TOF/MS, combined with principal component analysis (PCA). Nine ions were chosen to characterize the similarities and differences in the responses to fever. The ion at m/z 206.0278 was unambiguously identified to be xanthurenic acid. This study demonstrated that the metabonomic approach can readily distinguish between febrile and healthy individuals. This data support the contention that the metabonomic approach represents a promising new technology for the development of rapid-throughput in vivo fever screening. Furthermore, this approach can detect the interfering effects of RCE. This investigation has led the authors to believe that metabonomics is a valid approach for explaining the therapeutic effects of traditional Chinese medicine on fevers.
authors.affiliation: Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, P. R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Sun
authors.forename: Pingping
authors.initials: P
authors.lastname: Chen
authors.forename: Wanru
authors.initials: W
authors.lastname: Dong
mesh_terms: D019297:2,4-Dinitrophenol; D000818:Animals; D058633:Antipyretics / Q000627:therapeutic use*; D015415:Biomarkers / Q000652:urine; D039323:Coptis; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy / Q000652:urine*; D055432:Metabolomics / Q000379:methods; D008517:Phytotherapy; D025341:Principal Component Analysis; D051381:Rats; D017208:Rats, Wistar; D027343:Rhizome; D014977:Xanthurenates / Q000652:urine*
chemical_list: D058633:Antipyretics; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D014977:Xanthurenates; C028330:xanthurenic acid; D019297:2,4-Dinitrophenol