title: Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats.
abstract: OBJECTIVE
  To study the antiovulatory and abortifacient effects of ethanolic extract of Areca catechu in female rats.
  
  
  METHODS
  For antiovulatory effect, ethanolic extract of A. catechu at 100 and 300 mg/kg doses was administered orally for 15 days. Vaginal smears were examined daily microscopically for estrus cycle. Rats were sacrificed on 16(th) day. Ovarian weight, cholesterol estimation, and histopathological studies were done. Abortifacient activity was studied in rats at 100 and 300 mg/kg doses administered orally from 6(th) to 15(th) day of pregnancy. Rats were laparotomised on 19(th) day. The number of implantation sites and live fetuses were observed in both horns of the uterus.
  
  
  RESULTS
  The extract of A. catechu showed a significant decrease in the duration of estrus at 100 mg/kg (P = 0.015) and 300 mg/kg doses (P = 0.002) as compared with control. Metestrus phase was also significantly reduced at 100 mg/kg (P = 0.024) and 300 mg/kg doses (P = 0.002). There was a significant increase in proestrus (P < 0.001) phase. However, diestrus phase was unchanged. Histopathological study of the ovaries showed mainly primordial, primary, and secondary follicles in the test groups as compared to control. There was also a significant (P = 0.002) decrease in ovarian weight and a significant (P = 0.021) increase in ovarian cholesterol level at 100 mg/kg dose. In the study to evaluate abortifacient effect, the mean percentage of abortion with 100 and 300 mg/kg doses were 75.5% and 72.22%, respectively, which was significantly (P = 0.008 and P = 0.006, respectively) increased when compared with control.
  
  
  CONCLUSIONS
  The ethanolic extract of A. catechu at doses of 100 and 300 mg/kg has antiovulatory and abortifacient effects.
authors.affiliation: Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Shrestha
authors.forename: Tara
authors.initials: T
authors.lastname: Shanbhag
authors.forename: Smita
authors.initials: S
authors.lastname: Shenoy
authors.forename: Arul
authors.initials: A
authors.lastname: Amuthan
authors.forename: Krishnananda
authors.initials: K
authors.lastname: Prabhu
authors.forename: Stuti
authors.initials: S
authors.lastname: Sharma
authors.forename: Samik
authors.initials: S
authors.lastname: Banerjee
authors.forename: Sajala
authors.initials: S
authors.lastname: Kafle