title: Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L.
abstract: Two isolates of mulberry-pathogenic bacteria isolated from diseased mulberry roots were investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated strains R18-2(T) and R3-3 to the genus Enterobacter, with Enterobacter asburiae, E. amnigenus, E. cancerogenus, E. cloacae subsp. cloacae, E. cloacae subsp. dissolvens and E. nimipressuralis as their closest relatives. Cells of the isolates were Gram-negative, facultatively anaerobic rods, 0.3-1.0 µm wide and 0.8-2.0 µm long, with peritrichous flagella, showing a DNA G+C content of 55.1 ± 0.5 mol%. Calculation of a similarity index based on phenotypic features and fatty acid methyl ester (FAME) analysis suggested that these isolates are members of E. cancerogenus or E. asburiae or a closely related species. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by the presence of lysine decarboxylase activity and their ability to utilize d-arabitol. DNA-DNA relatedness also distinguished the two isolates from phylogenetically closely related Enterobacter strains. Based on these data, it is proposed that the isolates represent a novel species of the genus Enterobacter, named Enterobacter mori sp. nov. The type strain is R18-2(T) ( = CGMCC 1.10322(T) = LMG 25706(T)).
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Lou
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Guan-Lin
authors.initials: GL
authors.lastname: Xie
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Guo-Fen
authors.initials: GF
authors.lastname: Wang
authors.affiliation: Economic Crop Management Bureau, Zhejiang Provincial Agricultural Department, Hangzhou 310029, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Su
authors.affiliation: Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: USDA-ARS US Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.
authors.forename: Yong-Pin
authors.initials: YP
authors.lastname: Duan
mesh_terms: D001482:Base Composition; D004754:Enterobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D031625:Morus / Q000382:microbiology*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology
chemical_list: D005227:Fatty Acids