title: Expression of hypoxia inducible factor-1α and its significance in laryngeal carcinoma.
abstract: This study examined levels of hypoxia inducible factor-1α (HIF-1α) protein in 40 laryngeal squamous cell carcinoma specimens using immunohistochemistry. Correlations between HIF-1α immunoreactivity and patient age, tumour lymph node metastasis stage, histological grade (extent of differentiation), and alcohol and smoking history were evaluated. Of the tumour tissues obtained, 35 (87.5%) were located in the glottic area and five (12.5%) in the supraglottic area. All patients were male and aged between 35 and 71 years; 12 (30.0%) presented with lymph node metastases, 24 (60.0%) had cancer classified as T(1) or T(2), and 16 (40.0%) as high clinical stage (T(3) or T(4)). The pattern of HIF-1α protein localization in tumour tissues, when present, was mixed nuclear/cytoplasmic, with positive HIF-1α expression in 27 patients (67.5%). Differences in HIF-1α levels in samples from different tumour stages and in those with lymph node-positive versus lymph node-negative cancers were statistically significant.
authors.affiliation: Department of Otolaryngology, The People's Hospital of Deqing County, Zhejiang University, Deqing, China.
authors.forename: X-H
authors.initials: XH
authors.lastname: Wu
authors.forename: Y-F
authors.initials: YF
authors.lastname: Lu
authors.forename: X-D
authors.initials: XD
authors.lastname: Hu
authors.forename: J-Y
authors.initials: JY
authors.lastname: Mao
authors.forename: X-X
authors.initials: XX
authors.lastname: Ji
authors.forename: H-T
authors.initials: HT
authors.lastname: Yao
authors.forename: S-H
authors.initials: SH
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit