title: Molecular cloning and sequence analysis of two distinct halotolerant extracellular proteases from Bacillus subtilis FP-133.
abstract: We cloned the genes encoding the two distinct extracellular halotolerant proteases of Bacillus subtilis FP-133 Expro-I and Expro-II, which were classified as alkaline serine and neutral proteases respectively. Three-dimensional modeling suggested that acidic and polar amino acid residues located on the surface stabilize protein structure in the presence of relatively high NaCl concentrations.
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan. hakko3@kobe-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Takenaka
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Yoshida
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yoshida
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Murakami
authors.forename: Kenji
authors.initials: K
authors.lastname: Aoki
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000166:cytology / Q000201:enzymology* / Q000502:physiology; D003001:Cloning, Molecular; D005110:Extracellular Space / Q000201:enzymology*; D006868:Hydrolysis; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D055049:Salt Tolerance; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D012697:Serine Endopeptidases