title: Hierarchical gene synthesis using DNA microchip oligonucleotides.
abstract: High-cost of oligonucleotides is one of the major problems to low-cost gene synthesis. Although DNA oligonucleotides from cleavable DNA microchips has been adopted for the low-cost gene synthesis, construction of DNA molecules larger than 1 kb has been largely hampered due to the difficulties of DNA assembly associated with the negligible quantity of chip oligonucleotides. Here we report a hierarchical method for the synthesis of large genes using oligonucleotides from programmable DNA microchips. Using this hierarchical method, we successfully synthesized 1056 bp Dpo4 and 2325 bp Pfu DNA polymerase genes as models. This hierarchical strategy can be further expanded for the syntheses of multiple large genes in a scalable manner.
authors.affiliation: Department of Chemistry, Yonsei University, Shinchon 134, Seoul 120-749 Republic of Korea.
authors.forename: Hwangbeom
authors.initials: H
authors.lastname: Kim
authors.forename: Jaehwan
authors.initials: J
authors.lastname: Jeong
authors.forename: Duhee
authors.initials: D
authors.lastname: Bang
mesh_terms: D004247:DNA / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005813:Genes, Synthetic; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000191:economics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase