title: Endonucleases.
abstract: Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also provided. For a detailed discussion of the endonucleases more typically associated with DNA damage repair (e.g., Endo III, IV, V and VIII of E. coli and human APE1), see UNIT 3.9.
authors.affiliation: New England Biolabs, Inc., Ipswich, MA, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Nichols
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D005821:Genetic Techniques; D008836:Micrococcal Nuclease / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; C526101:Mung Bean Nuclease; D010940:Plant Proteins; D004247:DNA; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C038407:exonuclease Bal 31; D003850:Deoxyribonuclease I; C526098:Endonuclease S1, Aspergillus; D015719:Single-Strand Specific DNA and RNA Endonucleases; D008836:Micrococcal Nuclease