title: Synthesis, characterization, antibacterial activity, SOD mimic and interaction with DNA of drug based copper(II) complexes.
abstract: Novel metal complexes of the second-generation quinolone antibacterial agent enrofloxacin with copper(II) and neutral bidentate ligands have been prepared and characterized with elemental analysis reflectance, IR and mass spectroscopy. Complexes have been screened for their in-vitro antibacterial activity against two Gram(+ve) Staphylococcus aureus, Bacillus subtilis, and three Gram((-ve)) Serratia marcescens, Escherichia coli and Pseudomonas aeruginosa organisms using the double dilution technique. The binding of this complex with CT-DNA has been investigated by absorption titration, salt effect and viscosity measurements. Binding constant is ranging from 1.3×10(4)-3.7×10(4). The cleavage ability of complexes has been assessed by gel electrophoresis using pUC19 DNA. The catalytic activity of the copper(II) complexes towards the superoxide anion (O2.-) dismutation was assayed by their ability to inhibit the reduction of nitroblue tetrazolium (NBT).
authors.affiliation: Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India. jeenen@gmail.com
authors.forename: Mohan N
authors.initials: MN
authors.lastname: Patel
authors.forename: Promise A
authors.initials: PA
authors.lastname: Dosi
authors.forename: Bhupesh S
authors.initials: BS
authors.lastname: Bhatt
authors.forename: Vasudev R
authors.initials: VR
authors.lastname: Thakkar
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D003300:Copper / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008280:Magnetics; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D013055:Spectrophotometry, Infrared; D013482:Superoxide Dismutase / Q000378:metabolism*; D013816:Thermodynamics; D013997:Time Factors; D017186:Titrimetry; D014783:Viscosity / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D013482:Superoxide Dismutase