title: Effects of a Citrus depressa Hayata (shiikuwasa) extract on obesity in high-fat diet-induced obese mice.
abstract: Citrus depressa Hayata (commonly known as shiikuwasa) is cultivated in the northern areas of Okinawa, Japan, and used as a juice. In this study, we examined the anti-obesity effects and mechanism of action of shiikuwasa peel extract (SE) using high-fat diet (HFD)-induced obese mice. Mice were fed a low-fat diet (LFD), HFD or HFD containing 1% or 1.5% (w/w) SE (HFD+1 SE and HFD+1.5 SE, respectively) for 5 weeks. The body weight gain and white adipose tissue weight were significantly decreased in the HFD+1.5 SE group compared with the HFD group. The plasma triglyceride and leptin levels were also significantly reduced in the HFD+1.5 SE group compared with the HFD group. Histological examinations showed that the sizes of the adipocytes were significantly smaller in the HFD+1.5 SE group than in the HFD group. The HFD+1.5 SE group also showed significantly lower mRNA levels of lipogenesis-related genes, such as activating protein 2, stearoyl-CoA desaturase 1, acetyl-CoA-carboxylase 1, fatty acid transport protein and diacylglycerol acyltransferase 1, than the HFD group. These results suggest that the anti-obesity effects of SE may be elicited by regulating the expressions of lipogenesis-related genes in white adipose tissue.
authors.affiliation: Research Institute for Biological Functions, Chubu University, Kasugai, Aichi, Japan.
authors.forename: Young-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Byung-Yoon
authors.initials: BY
authors.lastname: Cha
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Saito
authors.forename: Sun-Sil
authors.initials: SS
authors.lastname: Choi
authors.forename: Xiao Xing
authors.initials: XX
authors.lastname: Wang
authors.forename: Bong-Keun
authors.initials: BK
authors.lastname: Choi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Teruya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagai
authors.forename: Je-Tae
authors.initials: JT
authors.lastname: Woo
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology; D002957:Citrus / Q000737:chemistry*; D004041:Dietary Fats / Q000008:administration & dosage; D005786:Gene Expression Regulation / Q000187:drug effects; D050155:Lipogenesis; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D019440:Anti-Obesity Agents; D004041:Dietary Fats; D010936:Plant Extracts