title: Serum cytokeratin 18 M30 antigen level and its correlation with nutritional parameters in middle-aged Japanese males with nonalcoholic fatty liver disease (NAFLD).
abstract: Cytokeratin (CK) 18 M30 antigen has been proposed as a diagnostic marker of nonalcoholic fatty liver disease (NAFLD). We studied serum CK18 M30 antigen level and examined the correlations among CK18 and biological data, dietary intake, and plasma fatty acid composition in middle-aged Japanese males with (NAFLD; n=42) and without NAFLD (control; n=35). NAFLD was diagnosed if subjects showed fatty liver on abdominal ultrasonography and their alcohol consumption was <20 g/d. They were also confirmed to have negative serological results for tests of autoimmune liver disease and hepatitis B and C. In the NAFLD group, body mass index, waist circumference, serum M30 antigen, alanine transaminase (ALT), cholinesterase, triacylglycerol, LDL-cholesterol, and HbA1c were significantly higher than in the control group. In the fatty acid analysis of plasma phospholipids, significantly higher dihomo-γ-linolenic acid (DGLA), total saturated fatty acids (SFA), and palmitic/linoleic acid ratio as well as lower arachidonic acid/DGLA ratio were observed in the NAFLD group compared with the control group. In the NAFLD group, M30 antigen was correlated positively with serum ALT, plasma DGLA, dietary SFA, and serum TNF-α as determined by partial correlation analysis controlled for BMI. On the basis of multivariate regression analysis using a stepwise method, M30 antigen was significantly associated with ALT and plasma DGLA. Regarding the determinants of NAFLD as revealed by logistic regression analysis, a high odds ratio was observed for plasma DGLA. In conclusion, members of the NAFLD group showed higher levels of serum CK18 M30 antigen and M30 antigen was strongly associated with serum ALT and plasma DGLA. Abnormal fatty acid metabolism may be a factor that causes aggravation of NAFLD.
authors.affiliation: Department of Food Science and Nutrition, Nara Women's University, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tabuchi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Tomioka
authors.forename: Takayo
authors.initials: T
authors.lastname: Kawakami
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Murakami
authors.forename: Makoto
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Itoshima
authors.forename: Shiori
authors.initials: S
authors.lastname: Sugawara
authors.forename: Aiko
authors.initials: A
authors.lastname: Kawashima
authors.forename: Misako
authors.initials: M
authors.lastname: Okita
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Tsukamoto
mesh_terms: D015126:8,11,14-Eicosatrienoic Acid; D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D044466:Asian People; D015992:Body Mass Index; D004032:Diet; D004435:Eating; D005227:Fatty Acids / Q000097:blood; D005234:Fatty Liver / Q000097:blood / Q000453:epidemiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D053538:Keratin-18 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D065626:Non-alcoholic Fatty Liver Disease; D012044:Regression Analysis; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055105:Waist Circumference
chemical_list: D005227:Fatty Acids; D053538:Keratin-18; D014409:Tumor Necrosis Factor-alpha; D000410:Alanine Transaminase; D015126:8,11,14-Eicosatrienoic Acid