title: [Therapeutic effects of tolerogenic dendritic cells on ovalbumin allergic BALB/c mice].
abstract: OBJECTIVE
  To investigate the therapeutic effects of AdCTLA4 Ig/Adα4β7-dendritic cell (DC) in the ovalbumin (OVA) allergic BALB/c mice.
  
  
  METHODS
  Twenty-four Female BALB/c mice aged 4-6 weeks fed on the ovalbumin-free feed were randomly divided into 3 groups: (1) treatment group: the OVA sensitized mice were injected with tolerogenic AdCTLA4-Ig/Adα4β7-DC after being challenged with OVA. (2) negative control group: mice were sensitized and challenged with normal saline. (3) positive control group: mice were sensitized and challenged with OVA. The level of the OVA-specific IgE in serum was measured by ELISA. The jejunal samples were observed histologically after HE staining. The level of IL-10 and TGF-β in murine intestine was detected by immunohistochemical and immunofluorescent methods, respectively.
  
  
  RESULTS
  Compared with the positive group, caudal vein injection with AdCTLA4Ig/Adα4β7-DC in treatment group reduced the level of the OVA-specific IgE in the serum remarkably, inhibited the inflammatory reactions of the intestine and increased the expression of the IL-10 in intestine significantly. There was no significant change in the expression of TGF-β in each group.
  
  
  CONCLUSIONS
  This study demonstrated that caudal vein injection with tolerogenic AdTLA4Ig/Adα4β7-DC may be of potential research value in the treatment of food allergy by inducing immune tolerance in vivo.
authors.affiliation: Department of Child Health Care, Chongqing Medical University, Chongqing 400014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Qian
authors.initials: Q
authors.lastname: Cheng
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D021181:Egg Hypersensitivity / Q000628:therapy*; D005260:Female; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation; D016753:Interleukin-10 / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000009:adverse effects*; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: C556062:IL10 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007073:Immunoglobulin E; D010047:Ovalbumin