title: Ionising irradiation-induced inhibition of differentiation of C3H10T1/2 cells to the osteoblastic lineage.
abstract: OBJECTIVE
  Previous studies using mouse osteoblast derived MC3T3-E1 and mouse myoblast derived C2C12 cells have not completely explained the mechanisms responsible for osteoradionecrosis. Thus, the aim of this study was to advance the in vitro experimental approaches for investigations of osteoradionecrosis.
  
  
  METHODS
  The pluripotent stem cell line, mouse embryo derived C3H10T1/2, was treated with all-trans-retinoic acid after irradiation (1, 3 and 6 Gy), and cell growth, cell cycle distribution, apoptosis, and alkaline phosphatase (ALP) activity were assessed.
  
  
  RESULTS
  We demonstrated that ionising radiation inhibited the growth and decreased ALP activity in C3H10T1/2 cells. The decrease in cell growth was not due to apoptosis but was due to cell cycle delay. The decrease in ALP activity persisted in cells that were induced to an osteoblastic lineage 24 h after irradiation.
  
  
  CONCLUSIONS
  Our results suggested that C3H10T1/2 cells are suitable for investigating the effects of ionising irradiation on osteoblast precursor cells.
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University Gokasho, Uji, Japan. sakurai@rish.kyoto-u.ac.jp
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sakurai
authors.forename: Michitoshi
authors.initials: M
authors.lastname: Ito
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mikamoto
authors.forename: Ryousuke
authors.initials: R
authors.lastname: Ohshio
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D002454:Cell Differentiation; D019070:Cell Lineage; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D008809:Mice, Inbred C3H; D032446:Myoblasts / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology* / Q000528:radiation effects*; D039904:Pluripotent Stem Cells / Q000166:cytology; D011839:Radiation, Ionizing; D014965:X-Rays
chemical_list: D000469:Alkaline Phosphatase