title: New methods for detection of campylobacters in stool samples in comparison to culture.
abstract: Campylobacter species, especially Campylobacter jejuni and Campylobacter coli, are a major cause of human bacterial enteritis. Current detection in stools is done essentially by culture on selective and nonselective media with filtration. These methods were compared to 2 molecular biology methods, an in-house real-time PCR and a multiplex PCR named Seeplex Diarrhea ACE Detection, and 3 immunoenzymatic methods, Premier Campy, RidaScreen Campylobacter, and ImmunoCard Stat!Campy. Out of 242 stool specimens tested, 23 (9.5%) fulfilled the positivity criteria, i.e., they were positive by one or both culture methods or, in case of a negative culture, by a positive molecular method and a positive immunoenzymatic method. The striking feature of this study is the low sensitivity of culture, in the range of 60%, in contrast to immunoenzymatic and molecular tests.
authors.affiliation: C.H.U. de Bordeaux, Hôpital Pellegrin, Laboratoire de Bactériologie, 33076 Bordeaux Cedex, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Bessède
authors.forename: Adline
authors.initials: A
authors.lastname: Delcamp
authors.forename: Elodie
authors.initials: E
authors.lastname: Sifré
authors.forename: Alice
authors.initials: A
authors.lastname: Buissonnière
authors.forename: Francis
authors.initials: F
authors.lastname: Mégraud
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques / Q000379:methods*; D002169:Campylobacter Infections / Q000175:diagnosis*; D017000:Campylobacter coli / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods; D012680:Sensitivity and Specificity