title: RP-HPLC analysis of Jirakadyarishta and chemical changes during fermentation.
abstract: Jirakadyarishta, an Ayurvedic formulation prepared by the fermentation of a decoction of Cuminum cyminum (seeds) is traditionally used for intestinal disorders. RP-HPLC analysis of the decoction and the final processed formulation revealed that apigenin-7-O-[galacturonide (1 --> 4)-O-glucoside] and luteolin-4'-O-glucoside-7-O-galacturonide) were the two major constituents of the decoction of C. cyminum. Selective hydrolysis of 7-O-glucosides of luteolin and apigenin during fermentation resulted in an increase in the amount of luteolin and apigenin. The 4'-O-glucoside-7-O-galacturonide of luteolin and galacturonide derivative of apigenin were not hydrolyzed during fermentation. Monomeric phenolics, together with 5-hydroxymethyl furfural (5-HMF), were also introduced into the formulation through the jaggery and other plant materials during fermentation. This communication highlights the importance of the ancient processing methods used in Ayurveda.
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. A. S. Nagar 160062, PB, India.
authors.forename: Uma Ranjan
authors.initials: UR
authors.lastname: Lal
authors.forename: Shailendra Mani
authors.initials: SM
authors.lastname: Tripathi
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Jachak
authors.forename: Kamlesh Kumar
authors.initials: KK
authors.lastname: Bhutani
authors.forename: Inder Pal
authors.initials: IP
authors.lastname: Singh
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D028527:Cuminum / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D008515:Medicine, Ayurvedic
chemical_list: D000975:Antioxidants