title: A method for CP 47, 497 a synthetic non-traditional cannabinoid in human urine using liquid chromatography tandem mass spectrometry.
abstract: A rapid method has been developed to analyse CP 47, 497 in human urine. Urine samples were diluted with water:acetonitrile (90:10, v/v) and sample aliquots were analysed by triple quadrupole tandem mass spectrometry with a runtime of 5 min. Multiple reaction monitoring (MRM) as survey scan was performed. The method was validated in urine, according to an in-house validation protocol based on the criteria defined in Commission Decision 2002/657/EC. Three MRM transitions were monitored. The decision limit (CCα) was 0.01μg mL⁻¹ and for the detection capability a (CCβ) value of 0.02 μg mL⁻¹ was obtained. The measurement uncertainty of the method was 21%. Fortifying human urine samples (n=18) in three separate assays, show the accuracy of the method to be between 95 and 96%. The precision of the method, expressed as RSD values for the within-lab reproducibility at the three levels of fortification (0.1, 0.15 and 0.2 μg mL⁻¹) was less than 10% respectively. The method proved to be simple, robust and time efficient. To the best of our knowledge there are no LC-MS methods for the determination of CP 47, 497 with validation data in urine.
authors.affiliation: The State Laboratory, Backweston Laboratory Complex, Young's Cross, Celbridge, Co., Kildare, Ireland. Geraldine.Dowling@statelab.ie
authors.forename: Geraldine
authors.initials: G
authors.lastname: Dowling
authors.forename: Liam
authors.initials: L
authors.lastname: Regan
mesh_terms: D002186:Cannabinoids / Q000737:chemistry / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D003511:Cyclohexanols / Q000737:chemistry / Q000652:urine*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002186:Cannabinoids; D003511:Cyclohexanols; C036088:CP 47497