title: [Relationship between 666C > T polymorphism of TIMP-1 and lumbar intervertebral disc degeneration].
abstract: OBJECTIVE
  To detect the single nucleotide polymorphisms (SNPs) in exon 6 of tissue inhibitor of metalloproteinase-1 (TIMP-1) in young men in North China and to investigate the relationship between these polymorphisms and lumbar intervertebral disc degeneration.
  
  
  METHODS
  The recruited subjects aged from 18 to 40 years old were divided by MRI into degeneration group (n = 48) and non-degeneration group (n = 49). The genomic DNA was collected from blood. After PCR amplification, the SNPs of TIMP-1 exon 6 were detected by DNA sequencing.
  
  
  RESULTS
  Only the TIMP-1 666C > T (rs11551797) polymorphism was observed and the genotype was of homozygote (CC/TT). The difference between two group was statistically significant (χ(2) = 4.571, P = 0.033). Although the polymorphism was a synonymous mutation, the TT genotype mutant type was one of the risk factors for lumbar intervertebral disc degeneration (OR = 3.269, 95%CI 1.063 - 10.047). In the degeneration group, no significant correlation was found between the polymorphism, extent, level and number of degenerated intervertebral discs.
  
  
  CONCLUSIONS
  TIMP-1 666C > T polymorphism present in north Chinese young men may lead to lumbar intervertebral disc degeneration. However it has no effect on the degree, level and number of degenerated intervertebral discs.
authors.affiliation: Department of Spinal Surgery, Fourth Clinical College of Peking University, Beijing 100035, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.forename: Dan-hui
authors.initials: DH
authors.lastname: Zhao
authors.forename: Cheng-ai
authors.initials: CA
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Yan-zhuo
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D005838:Genotype; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000235:genetics*; D008159:Lumbar Vertebrae; D008297:Male; D020641:Polymorphism, Single Nucleotide; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D055815:Young Adult
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1