title: Application of experimental design in optimization of the separation condition for determination of four active components in cold medicines by flow injection-capillary electrophoresis.
abstract: Orthogonal design (OD) was employed to optimize the separation condition of flow injection-capillary electrophoresis (FI-CE). In order to compare the optimum condition, uniform design and univariate approach were also adopted. The influences of variables such as buffer pH, buffer concentration, acetonitrile (ACN) percentage, and separation voltage were discussed. The optimum separation condition was established. The limits of detection were 1.94 × 10(-2), 6.40 × 10(-3), 1.16 × 10(-2) and 1.94 × 10(-2) μg/mL for dextromethorphan hydrobromide (Dex), chlorphenamine hydrogen maleate (Chl), pseudoephedrine hydrochloride (Pse), and paracetamol (Par), respectively. The RSDs of peaks areas were less than 2.0%. The results showed the OD was an effective method among experimental designs for optimizing the separation conditions of CE. The optimum condition was used for separation and determination of Dex, Chl, Pse, and Par in cold medicines. The average recovery was between 96.68-101.25%.
authors.affiliation: School of Pharmacy, Shandong University, Jinan 250012, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Liu
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D000082:Acetaminophen / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000704:Analysis of Variance; D002744:Chlorpheniramine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D003915:Dextromethorphan / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods*; D057985:Multi-Ingredient Cold, Flu, and Allergy Medications / Q000737:chemistry*; D054199:Pseudoephedrine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D057985:Multi-Ingredient Cold, Flu, and Allergy Medications; D000082:Acetaminophen; D002744:Chlorpheniramine; D003915:Dextromethorphan; D054199:Pseudoephedrine