title: Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil.
abstract: A Gram-negative, pink-pigmented, non-spore-forming rod shaped, methanol-utilizing bacterium, strain YIM 48816(T), was isolated from forest soil collected from Sichuan province, China. Strain YIM 48816(T) can grow at 4-37 °C, pH 5.0-7.0 and 0% NaCl (w/v). Based on 16S rRNA gene sequence similarity studies, it belonged to the genus Methylobacterium, and formed a phyletic line. The 16S rRNA gene sequence similarities were 96.2% to Methylobacterium mesophilicum DSM 1708(T) and 96.0% to Methylobacterium brachiatum DSM 19569(T), and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 96.0%. The major menaquinones detected were Q-10 (97.14%) and Q-9 (2.86%). The major fatty acids were C18:1 ω7c (80.84%). The DNA G + C content was 66.2 mol%. It is apparent from the genotypic and phenotypic data that strain YIM 48816(T) belongs to a novel species of the genus Methylobacterium, for which the name Methylobacterium soli sp. nov. is proposed. The type strain is YIM 48816(T) (CCTCC AA 208027(T) = KCTC 22810(T)).
authors.affiliation: College of Resources and Environment, Northwest A&F University, Yangling, 712100 Xianyang, Shaanxi, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Cao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Rong-Xian
authors.initials: RX
authors.lastname: Jin
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: He
authors.forename: Hang-Xian
authors.initials: HX
authors.lastname: Lai
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D000432:Methanol / Q000378:metabolism*; D020580:Methylobacterium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014197:Trees
chemical_list: D012336:RNA, Ribosomal, 16S; D000432:Methanol