title: [Substrate specificity of carotenoid 3',4'-desaturase from Deinococcus radiodurans].
abstract: To examine the substrate specificity of carotenoid 3',4'-desaturase (DR2250) from Deinococcus radiodurans, we amplified the dr2250 gene by using PCR methods. The PCR products were digested by Hind III-BamH I and ligated into the vector pUC19, yielding recombinant vector pUC-CRTD. We analyzed the carotenoids of E. coli transformants containing pACCRT-EBI(Eu) and (or) pRK-CRTC and (or) pUC-CRTD. Our results demonstrated that DR2250 had substrate specificity on the carotenoids with hydroxyl group at C1 (1').
authors.affiliation: Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Zongtao
authors.initials: Z
authors.lastname: Sun
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.forename: Shaochuan
authors.initials: S
authors.lastname: Shen
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Hu
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D034301:Deinococcus / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: C547822:deinoxanthin; D002338:Carotenoids; D010088:Oxidoreductases; C022398:phytoene dehydrogenase