title: Transactivation of human parvovirus B19 gene expression in endothelial cells by adenoviral helper functions.
abstract: Human parvovirus B19 (B19V) DNA is highly prevalent in endothelial cells lining up intramyocardial arterioles and postcapillary venules of patients with chronic myocarditis and cardiomyopathies. We addressed the question of a possible stimulation of B19V gene expression in endothelial cells by infection with adenoviruses. Adenovirus infection led to a strong augmentation of B19V structural and nonstructural proteins in individual endothelial cells infected with B19V or transfected with an infectious B19V genome. Transactivation was mostly mediated at the level of transcription and not due to adenovirus-mediated induction of second-strand synthesis from the single-stranded parvoviral genome. The main adenoviral functions required were E1A and E4orf6, which displayed synergistic effects. Furthermore, a limited B19V genome replication could be demonstrated in endothelial cells and adenovirus infection induced the appearance of putative dimeric replication intermediates. Thus the almost complete block in B19V gene expression seen in endothelial cells can be abrogated by infection with other viruses.
authors.affiliation: Department of Cardiology and Pneumology, Campus Benjamin Franklin, Charité-University Medicine Berlin, Hindenburgdamm 30, 12200 Berlin, Germany. tanja.pozzuto@charite.de
authors.forename: Tanja
authors.initials: T
authors.lastname: Pozzuto
authors.forename: Kristina
authors.initials: K
authors.lastname: von Kietzell
authors.forename: Thomas
authors.initials: T
authors.lastname: Bock
authors.forename: Caroline
authors.initials: C
authors.lastname: Schmidt-Lucke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Poller
authors.forename: Thomas
authors.initials: T
authors.lastname: Zobel
authors.forename: Dirk
authors.initials: D
authors.lastname: Lassner
authors.forename: Heinz
authors.initials: H
authors.lastname: Zeichhardt
authors.forename: Stefan
authors.initials: S
authors.lastname: Weger
authors.forename: Henry
authors.initials: H
authors.lastname: Fechner
mesh_terms: D017500:Adenovirus E1A Proteins / Q000378:metabolism; D017504:Adenovirus E4 Proteins / Q000378:metabolism; D000260:Adenoviruses, Human / Q000235:genetics*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006801:Humans; D016732:Parvovirus B19, Human / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D017500:Adenovirus E1A Proteins; D017504:Adenovirus E4 Proteins; C553318:E4orf6 protein, adenovirus; D015534:Trans-Activators; D014764:Viral Proteins