title: Epigenetic regulation of transposable element derived human gene promoters.
abstract: It was previously thought that epigenetic histone modifications of mammalian transposable elements (TEs) serve primarily to defend the genome against deleterious effects associated with their activity. However, we recently showed that, genome-wide, human TEs can also be epigenetically modified in a manner consistent with their ability to regulate host genes. Here, we explore the ability of TE sequences to epigenetically regulate individual human genes by focusing on the histone modifications of promoter sequences derived from TEs. We found 1520 human genes that initiate transcription from within TE-derived promoter sequences. We evaluated the distributions of eight histone modifications across these TE-promoters, within and between the GM12878 and K562 cell lines, and related their modification status with the cell-type specific expression patterns of the genes that they regulate. TE-derived promoters are significantly enriched for active histone modifications, and depleted for repressive modifications, relative to the genomic background. Active histone modifications of TE-promoters peak at transcription start sites and are positively correlated with increasing expression within cell lines. Furthermore, differential modification of TE-derived promoters between cell lines is significantly correlated with differential gene expression. LTR-retrotransposon derived promoters in particular play a prominent role in mediating cell-type specific gene regulation, and a number of these LTR-promoter genes are implicated in lineage-specific cellular functions. The regulation of human genes mediated by histone modifications targeted to TE-derived promoters is consistent with the ability of TEs to contribute to the epigenomic landscape in a way that provides functional utility to the host genome.
authors.affiliation: School of Biology, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332, USA. ahsan.huda@gatech.edu
authors.forename: Ahsan
authors.initials: A
authors.lastname: Huda
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Bowen
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Conley
authors.forename: I King
authors.initials: IK
authors.lastname: Jordan
mesh_terms: D002460:Cell Line; D004251:DNA Transposable Elements / Q000235:genetics*; D057890:Epigenomics; D055106:Genome-Wide Association Study; D006657:Histones / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D006657:Histones