title: Intratumoral expression of IL-17 and its prognostic role in gastric adenocarcinoma patients.
abstract: In this study, we characterized the intratumoral expression of IL-17 and CD8(+) TILs in gastric adenocarcinoma patients after resection and determined the correlation between the survival probability of gastric adenocarcinoma patients and the expression of IL-17 in tumor. Expression of IL-17 and CD8 was assessed by immunohistochemistry, and the prognostic effects of intratumoral IL-17 expression and CD8(+) TILs were evaluated by Cox regression and Kaplan-Meier analysis. Immunohistochemical detection revealed the presence of IL-17 and CD8(+) cells in gastric adenocarcinoma tissue samples (90.6%, 174 out of 192 patients and 96.9%, 186 out of 192 patients, respectively). We have also found that intratumoral IL-17 expression was significantly correlated with age (p=0.004) and that the number of CD8(+)TILs was significantly correlated with UICC staging (p=0.012) and the depth of tumor invasion (p=0.022). The five-year overall survival probability among patients intratumorally expressing higher levels of IL-17 was significantly better than those expressing lower levels of IL-17 (p=0.036). Multivariate Cox proportional hazard analyses revealed that intratumoral IL-17 expression (HR: 0.521; 95% CI: 0.329-0.823; p=0.005) was an independent factor affecting the five-year overall survival probability. We conclude that low levels of intratumoral IL-17 expression may indicate poor prognosis in gastric adenocarcinoma patients.
authors.affiliation: The State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Cancer Center, Sun Yat-sen University, Guangzhou 510060, PR China.
authors.forename: Ju-gao
authors.initials: JG
authors.lastname: Chen
authors.forename: Jian-chuan
authors.initials: JC
authors.lastname: Xia
authors.forename: Xiao-ting
authors.initials: XT
authors.lastname: Liang
authors.forename: Ke
authors.initials: K
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Lv
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Qi-jing
authors.initials: QJ
authors.lastname: Wang
authors.forename: Yong-qiang
authors.initials: YQ
authors.lastname: Li
authors.forename: Shi-ping
authors.initials: SP
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.forename: Li-xi
authors.initials: LX
authors.lastname: Huang
authors.forename: Miao-la
authors.initials: ML
authors.lastname: Ke
authors.forename: Yi-bing
authors.initials: YB
authors.lastname: Chen
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Ma
authors.forename: Zhen-wu
authors.initials: ZW
authors.lastname: Zeng
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Zhou
authors.forename: Alfred E
authors.initials: AE
authors.lastname: Chang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality; D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D016827:CD8 Antigens / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020381:Interleukin-17 / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality
chemical_list: D016827:CD8 Antigens; D020381:Interleukin-17