title: WBC27, an adenosine tri-phosphate-binding cassette protein, controls pollen wall formation and patterning in Arabidopsis.
abstract: In flowering plants, the exine components are derived from tapetum. Despite its importance to sexual plant reproduction, little is known about the translocation of exine materials from tapetum to developing microspores. Here we report functional characterization of the arabidopsis WBC27 gene. WBC27 encodes an adenosine tri-phosphate binding cassette (ABC) transporter and is expressed preferentially in tapetum. Mutation of WBC27 disrupted the exine formation. The wbc27 mutant microspores began to degenerate once released from tetrads and most of the microspores collapsed at the uninucleate stage. Only a small number of wbc27-1 microspores could develop into tricellular pollen grains. These survival pollen grains lacked exine and germinated in the anther before anthesis. All of these results suggest that the ABC transporter, WBC27 plays important roles in the formation of arabidopsis exine, possibly by translocation of lipidic precursors of sporopollenin from tapetum to developing microspores.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultral University, Beijing, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Dou
authors.forename: Ke-Zhen
authors.initials: KZ
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Liu
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Chen
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.forename: De
authors.initials: D
authors.lastname: Ye
mesh_terms: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D019521:Body Patterning; D002462:Cell Membrane / Q000378:metabolism; D050356:Lipid Metabolism; D016254:Mutagenesis, Insertional; D010641:Phenotype; D011058:Pollen / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics
chemical_list: D000070996:ATP Binding Cassette Transporter, Subfamily G; D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D014157:Transcription Factors; C480845:AMS protein, Arabidopsis; C532649:TDF1 protein, Arabidopsis; C557486:WBC27 protein, Arabidopsis