title: Analysing cellulose biosynthesis with confocal microscopy.
abstract: Plant cells are delimited by a rigid cell wall that resists internal turgor pressure, but extends with a remarkable degree of control that allows the cell to grow and acquire specific shapes. Live cell fluorescence microscopy systems have allowed an amazing view into the complex and dynamic lives of individual proteins during cell morphogenesis. The current chapter will focus on methodology for live cell imaging of cellulose synthase (CESA) in Arabidopsis, which will also provide a launching pad to explore ones specific protein of interest.
authors.affiliation: Department of Horticulture, University of Kentucky, Lexington, KY, USA.
authors.forename: Meera
authors.initials: M
authors.lastname: Nair
authors.forename: Seth
authors.initials: S
authors.lastname: Debolt
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017796:Caulimovirus / Q000235:genetics; D002482:Cellulose / Q000096:biosynthesis*; D003001:Cloning, Molecular; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D018613:Microscopy, Confocal; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D036226:Seedlings / Q000166:cytology / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase