title: Hunting for protein markers of hypoxia by combining plasma membrane enrichment with a new approach to membrane protein analysis.
abstract: Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.
authors.affiliation: Department of Immunotechnology, CREATE Health, Lund University, Sweden.
authors.forename: Paolo
authors.initials: P
authors.lastname: Cifani
authors.forename: Maria
authors.initials: M
authors.lastname: Bendz
authors.forename: Kristofer
authors.initials: K
authors.lastname: Wårell
authors.forename: Karin
authors.initials: K
authors.lastname: Hansson
authors.forename: Fredrik
authors.initials: F
authors.lastname: Levander
authors.forename: Marianne
authors.initials: M
authors.lastname: Sandin
authors.forename: Morten
authors.initials: M
authors.lastname: Krogh
authors.forename: Marie
authors.initials: M
authors.lastname: Ovenberger
authors.forename: Erik
authors.initials: E
authors.lastname: Fredlund
authors.forename: Marica
authors.initials: M
authors.lastname: Vaapil
authors.forename: Alexander
authors.initials: A
authors.lastname: Pietras
authors.forename: Sven
authors.initials: S
authors.lastname: Påhlman
authors.forename: Peter
authors.initials: P
authors.lastname: James
mesh_terms: D015415:Biomarkers / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry*; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046228:Microarray Analysis; D009369:Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D012333:RNA, Messenger