title: Suppression of the temperature-sensitive mutation of the bamD gene required for the assembly of outer membrane proteins by multicopy of the yiaD gene in Escherichia coli.
abstract: We isolated temperature-sensitive mutants of the Escherichia coli bamD gene, which is essential for the assembly of β-barrel outer membrane proteins. As their multicopy suppressor, we identified a novel yiaD gene encoding a putative lipoprotein, YiaD. Mutations of its OmpA domain, which is required for interaction with peptidoglycan, affected suppression, suggesting that interaction with peptidoglycan is important to YiaD function.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tachikawa
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kato
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D018628:Gene Dosage / Q000235:genetics*; D008074:Lipoproteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D011485:Protein Binding; D013489:Suppression, Genetic; D013696:Temperature
chemical_list: D001425:Bacterial Outer Membrane Proteins; C513739:BamD protein, E coli; D029968:Escherichia coli Proteins; D008074:Lipoproteins