title: Evaluation of an inhouse rapid ELISA test for detection of giardia in domestic sheep (Ovis aries).
abstract: Sheep (Ovis aries) are increasingly used at our institution as models of human disease. Within the research environment, routine husbandry and handling of sheep has potential for transmission of zoonotic agents, including Giardia. The prevalence of Giardia in sheep may approach 68%. Classic diagnostic testing involves microscopic examination for fecal cysts or trophozoites; however, limitations of microscopy include time, labor, and potential false-negative results due to intermittent shedding. We wished to determine whether a commercial rapid ELISA used for Giardia detection in dogs and cats could be used in sheep. Fecal samples collected from sheep (n = 93) were tested with a combination of 6 methods: reference laboratory fecal flotation, reference laboratory ELISA, inhouse fecal flotation, and commercially available tests (enzyme immunoassay, direct fluorescence antibody assay, and rapid ELISA). Prevalence of Giardia infection in facility sheep was 11.8% (11 of 93 animals). Of the 11 samples considered positive, 3 were confirmed by multiple testing methods, and 5 were positive by microscopy alone. Inhouse fecal flotation for 8 samples was positive on only 1 of 2 consecutive testing days. The rapid ELISA test exhibited 0% sensitivity for sheep giardiasis. Overall, the examined methods had low sensitivities and low positive predictive values. Despite limitations, microscopic analysis of repeat fecal samples remained the most accurate diagnostic method for ovine giardiasis among the methods tested.
authors.affiliation: University Laboratory Animal Resources, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. wilsonj2@email.chop.edu
authors.forename: Jolaine M
authors.initials: JM
authors.lastname: Wilson
authors.forename: F Claire
authors.initials: FC
authors.lastname: Hankenson
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary*; D005872:Giardia / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D010414:Pennsylvania / Q000453:epidemiology; D015995:Prevalence; D012680:Sensitivity and Specificity; D012757:Sheep Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D034561:Sheep, Domestic; D015047:Zoonoses / Q000469:parasitology