title: Congenital immune defense of the hair.
abstract: Studies of two recent decades provided ample data on antibacterial peptides protecting animal and human epithelium of different types; however, no reports about the presence of these compounds in hair keratinocytes appeared up to the present time. Peptides were extracted from specimens of normal intact hair with citric acid solution in 50% ethanol. Antibacterial activity of the resultant extracts was evaluated on Candida albicans test culture by staining and microscopy, by evaluation of growth inhibition zones, and by inoculations. Direct contact with the extract destroyed the greater part of Candida albicans cells up to complete destruction of membranes. Application of the extract to dishes with agar with Candida albicans led in the formation of apparent zones of growth inhibition. The percentage of killed cells increased with prolongation of incubation with the extract. Electrophoresis of hair extract showed bands characteristic of antibacterial peptides RNase, psoriasin, and β-defensins. Removal of the peptides from the extract by filtration through membrane filter led to loss of its activity. The results indicate that human hair keratinocytes possess congenital antibacterial immunity.
authors.affiliation: I I Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia. veraar@mail.ru
authors.forename: V G
authors.initials: VG
authors.lastname: Arzumanyan
authors.forename: N O
authors.initials: NO
authors.lastname: Vartanova
authors.forename: O A
authors.initials: OA
authors.lastname: Serdyuk
authors.forename: I M
authors.initials: IM
authors.lastname: Ozhovan
mesh_terms: D000293:Adolescent; D000328:Adult; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002648:Child; D005260:Female; D006197:Hair / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008875:Middle Aged
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents