title: Genetic mapping of DArT markers in the Festuca-Lolium complex and their use in freezing tolerance association analysis.
abstract: Species belonging to the Festuca-Lolium complex are important forage and turf species and as such, have been studied intensively. However, their out-crossing nature and limited availability of molecular markers make genetic studies difficult. Here, we report on saturation of F. pratensis and L. multiflorum genetic maps using Diversity Array Technology (DArT) markers and the DArTFest array.The 530 and 149 DArT markers were placed on genetic maps of L. multiflorum and F. pratensis, respectively, with overlap of 20 markers, which mapped in both species. The markers were sequenced and comparative sequence analysis was performed between L. multiflorum, rice and Brachypodium. The utility of the DArTFest array was then tested on a Festulolium population FuRs0357 in an integrated analysis using the DArT marker map positions to study associations between markers and freezing tolerance. Ninety six markers were significantly associated with freezing tolerance and five of these markers were genetically mapped to chromosomes 2, 4 and 7. Three genomic loci associated with freezing tolerance in the FuRs0357 population co-localized with chromosome segments and QTLs previously identified to be associated with freezing tolerance. The present work clearly confirms the potential of the DArTFest array in genetic studies of the Festuca-Lolium complex. The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca-Lolium species.
authors.affiliation: Institute of Experimental Botany, Sokolovská 6, 77200, Olomouc, Czech Republic. bartos@ueb.cas.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Bartoš
authors.forename: Simen Rød
authors.initials: SR
authors.lastname: Sandve
authors.forename: Roland
authors.initials: R
authors.lastname: Kölliker
authors.forename: David
authors.initials: D
authors.lastname: Kopecký
authors.forename: Pavla
authors.initials: P
authors.lastname: Christelová
authors.forename: Stěpán
authors.initials: S
authors.lastname: Stočes
authors.forename: Liv
authors.initials: L
authors.lastname: Ostrem
authors.forename: Arild
authors.initials: A
authors.lastname: Larsen
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kilian
authors.forename: Odd-Arne
authors.initials: OA
authors.lastname: Rognli
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D058431:Brachypodium / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant; D031751:Festuca / Q000235:genetics*; D005615:Freezing; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D008129:Lolium / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D026801:Synteny
chemical_list: D005819:Genetic Markers