title: Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp.
abstract: The cardiac action potential (AP) is shaped by myriad ionic currents. In this study, we develop an innovative AP-clamp Sequential Dissection technique to enable the recording of multiple ionic currents in the single cell under AP-clamp. This new technique presents a significant step beyond the traditional way of recording only one current in any one cell. The ability to measure many currents in a single cell has revealed two hitherto unknown characteristics of the ionic currents in cardiac cells: coordination of currents within a cell and large variation of currents between cells. Hence, the AP-clamp Sequential Dissection method provides a unique and powerful tool for studying individual cell electrophysiology.
authors.affiliation: Department of Pharmacology, University of California, Davis, CA 95616, USA.
authors.forename: Tamas
authors.initials: T
authors.lastname: Banyasz
authors.forename: Balazs
authors.initials: B
authors.lastname: Horvath
authors.forename: Zhong
authors.initials: Z
authors.lastname: Jian
authors.forename: Leighton T
authors.initials: LT
authors.lastname: Izu
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen-Izu
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D004210:Dissection / Q000379:methods; D006168:Guinea Pigs; D017136:Ion Transport / Q000502:physiology; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*