title: Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip.
abstract: We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, 2800 Kgs. Lyngby, Denmark.
authors.forename: David
authors.initials: D
authors.lastname: Selmeczi
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Hansen
authors.forename: Ozcan
authors.initials: O
authors.lastname: Met
authors.forename: Inge Marie
authors.initials: IM
authors.lastname: Svane
authors.forename: Niels B
authors.initials: NB
authors.lastname: Larsen
mesh_terms: D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D004209:Disposable Equipment; D018274:Electroporation / Q000295:instrumentation*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D013193:Stainless Steel / Q000737:chemistry; D014162:Transfection
chemical_list: D011108:Polymers; D012333:RNA, Messenger; D013193:Stainless Steel; D049452:Green Fluorescent Proteins