title: Design and optimization of fermentation medium for enhanced bacteriocin production by probiotic bacterium Enterococcus faecium MC13.
abstract: Statistics-based experimental designs were used to develop a cost-effective medium for enhanced production of viable cells and bacteriocin by probiotic Enterococcus faecium MC13. Carbon, nitrogen, and mineral sources were first screened by one-variable-at-a-time (OVAT) methods. In order to increase yield production, the selected variables were further statistically optimized using response-surface methodology (RSM) with central composite design (CCD). The maximum and minimum levels of the selected variables were determined and a set of 34 experimental runs was performed. The optimum concentrations of the tested variables for production of viable cells (12.24 log CFU mL(-1)) and bacteriocin activity (25,600 AU mL(-1)) were tryptone (10.0 g/L), peptone (6.0 g/L), maltose (3.0 g/L), glucose (9.0 g/L), NaCl (15.0 g/L), sodium citrate (2.5 g/L), sodium acetate (1.0 g/L), and dipotassium PO(4) (0.1 g/L). Threefold increased yield of bacteriocin was achieved in optimized medium compared to the unoptimized counterpart, and this was two times less cost than commercial MRS medium.
authors.affiliation: Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry, India.
authors.forename: Paulraj
authors.initials: P
authors.lastname: Kanmani
authors.forename: R Satish
authors.initials: RS
authors.lastname: Kumar
authors.forename: N
authors.initials: N
authors.lastname: Yuvaraj
authors.forename: K A
authors.initials: KA
authors.lastname: Paari
authors.forename: V
authors.initials: V
authors.lastname: Pattukumar
authors.forename: Venkatesan
authors.initials: V
authors.lastname: Arul
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis*; D002951:Citrates; D003470:Culture Media; D016984:Enterococcus faecium / Q000254:growth & development / Q000378:metabolism*; D005516:Food Microbiology; D005947:Glucose; D007218:Industrial Microbiology / Q000379:methods*; D008320:Maltose; D015233:Models, Statistical; D010461:Peptones; D010710:Phosphates; D017680:Potassium Compounds; D019936:Probiotics; D019346:Sodium Acetate; D012965:Sodium Chloride; D000077559:Sodium Citrate
chemical_list: D001430:Bacteriocins; D002951:Citrates; D003470:Culture Media; D010461:Peptones; D010710:Phosphates; D017680:Potassium Compounds; D000077559:Sodium Citrate; D012965:Sodium Chloride; D019346:Sodium Acetate; D008320:Maltose; C001830:tryptones; C013216:potassium phosphate; D005947:Glucose