title: Extraction and detection of arabinogalactan proteins.
abstract: Arabinogalactan proteins are a diverse group of plant cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localise, and quantify them is fundamentally important. This chapter describes two commonly used methods, histological staining and radial gel diffusion, both of which utilise the ability of Yariv reagent to bind to AGPs.
authors.affiliation: Botany and Plant Science, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland. zoe.popper@nuigalway.ie
authors.forename: Zoë A
authors.initials: ZA
authors.lastname: Popper
mesh_terms: D005960:Glucosides / Q000737:chemistry; D005779:Immunodiffusion; D007202:Indicators and Reagents; D009088:Mucoproteins / Q000737:chemistry / Q000302:isolation & purification*; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D018514:Plant Structures / Q000737:chemistry*; D011485:Protein Binding; D013194:Staining and Labeling
chemical_list: D005960:Glucosides; D007202:Indicators and Reagents; D009088:Mucoproteins; D010940:Plant Proteins; C104977:Yariv reagent; C006619:arabinogalactan proteins; D010696:Phloroglucinol