title: Simultaneous HPLC determination of thiocolchicoside and glafenine as well as thiocolchicoside and floctafenine in their combined dosage forms.
abstract: Sensitive and accurate high-performance liquid chromatographic methods have been developed for the simultaneous determination of thiocolchicoside (TC)-glafenine (GF) (Mix I) and thiocolchicoside-floctafenine (FN) (Mix II) in their pharmaceutical formulations. The analysis for both mixtures was performed using 250 mm × 4.6 mm i.d., 5 μm particle size C18 Waters Symmetry column. The mobile phase consisted of methanol-0.035 M phosphate buffer (50:50, v/v) of pH 4.5 for Mix I and methanol-0.03 M phosphate buffer (70:30, v/v) of pH 4 for Mix II with flow rate of 1 mL/min and UV detection at 400 nm in both cases. The calibration plots were rectilinear over the concentration range of 0.2-2 μg/mL for TC in both mixtures and 20-200 μg/mL for each of GF and FN . The limits of detection for TC and GF were 0.05 μg/mL and 0.62 μg/mL, respectively, and for TC and FN were 0.02 μg/mL and 0.70 μg/mL, respectively. Additionally, the proposed methods were successfully applied to their combined tablets with average percentage recoveries of 100.35 ± 0.61 and 100.57 ± 0.72% for TC and GF respectively and for TC and FN the percentage recoveries were 101.2 ± 0.72 and 100.36 ± 0.67%, respectively. The results obtained were favorably compared with those given using the comparison methods.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Walash
authors.forename: Fathalla
authors.initials: F
authors.lastname: Belal
authors.forename: Manal
authors.initials: M
authors.lastname: Eid
authors.forename: Samah Abo
authors.initials: SA
authors.lastname: el-Abass
mesh_terms: D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003078:Colchicine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004338:Drug Combinations; D005897:Glafenine / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D000432:Methanol; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D062367:ortho-Aminobenzoates / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004338:Drug Combinations; D062367:ortho-Aminobenzoates; D005897:Glafenine; C084600:floctafenine; D003078:Colchicine; C004280:thiocolchicoside; D000432:Methanol