title: Comparison of the properties of lipase immobilized onto mesoporous resins by different methods.
abstract: Genipin, a natural cross-linking agent, was used for the immobilization of lipase from Candida sp. 99-125 by cross-linking to two kinds of mesoporous resins. Under optimum conditions, the activity recovery of immobilized lipase on resin NKA-9 could reach up to 96.99% when the genipin concentration was 0.5%, and it could reach up to 86.18% for S-8 with a genipin concentration of 0.25%. Compared with using glutaraldehyde as a cross-linking agent, the immobilized lipase using genipin showed better pH and thermal stability, storage stability, and reusability. The residual activity of immobilized lipase using genipin as cross-linker remained more than 60% of its initial activity after six hydrolytic cycles, whereas only about 35% activity remained by using glutaraldehyde as cross-linker.
authors.affiliation: Department of Bioengineering, School of Chemical Engineering, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Wang
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Liya
authors.initials: L
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D002175:Candida / Q000201:enzymology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D005976:Glutaral / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057889:Iridoid Glycosides / Q000737:chemistry; D039823:Iridoids; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D057889:Iridoid Glycosides; D039823:Iridoids; D000069463:Olive Oil; D010938:Plant Oils; D012117:Resins, Synthetic; C007834:genipin; D008049:Lipase; D005976:Glutaral