title: Novel group A rotavirus G8 P[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs.
abstract: Rotavirus is a worldwide major cause of diarrhea outbreaks in neonatal ruminants. An outbreak of ovine diarrheic syndrome (ODS) in 50-75 days-old lambs (weaned lambs) is described. Fecal immunochromatography and intestinal immunohistochemistry for rotavirus group A were performed. In addition, semi-nested multiplex RT-PCR for G and P rotavirus genotyping in combination with sequencing were performed, to support the diagnosis and identify the viral strain. A novel ovine rotavirus group A G8 P[1] strain was determined as the main cause of the ODS observed, whereas other pathogens were ruled out.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. ivanjose.galindo@uab.es
authors.forename: I
authors.initials: I
authors.lastname: Galindo-Cardiel
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Jiménez
authors.forename: L
authors.initials: L
authors.lastname: Luján
authors.forename: J
authors.initials: J
authors.lastname: Buesa
authors.forename: J
authors.initials: J
authors.lastname: Espada
authors.forename: E
authors.initials: E
authors.lastname: Fantova
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: J
authors.initials: J
authors.lastname: Segalés
authors.forename: J J
authors.initials: JJ
authors.lastname: Badiola
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000662:veterinary; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005838:Genotype; D007150:Immunohistochemistry / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017423:Sequence Analysis, RNA; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000453:epidemiology / Q000821:virology*; D034561:Sheep, Domestic / Q000821:virology*; D013030:Spain / Q000453:epidemiology; D014886:Weaning
chemical_list: D012367:RNA, Viral