title: Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa.
abstract: The fucose-/mannose-specific lectin LecB from Pseudomonas aeruginosa is transported to the outer membrane; however, the mechanism used is not known so far. Here, we report that LecB is present in the periplasm of P. aeruginosa in two variants of different sizes. Both were functional and could be purified by their affinity to mannose. The difference in size was shown by a specific enzyme assay to be a result of N glycosylation, and inactivation of the glycosylation sites was shown by site-directed mutagenesis. Furthermore, we demonstrate that this glycosylation is required for the transport of LecB.
authors.affiliation: Institute of Microbiology and Biotechnology, Ulm University, Ulm, Germany.
authors.forename: Kai-Malte
authors.initials: KM
authors.lastname: Bartels
authors.forename: Horst
authors.initials: H
authors.lastname: Funken
authors.forename: Andreas
authors.initials: A
authors.lastname: Knapp
authors.forename: Melanie
authors.initials: M
authors.lastname: Brocker
authors.forename: Michael
authors.initials: M
authors.lastname: Bott
authors.forename: Susanne
authors.initials: S
authors.lastname: Wilhelm
authors.forename: Karl-Erich
authors.initials: KE
authors.lastname: Jaeger
authors.forename: Frank
authors.initials: F
authors.lastname: Rosenau
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D006031:Glycosylation; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D019897:Periplasm / Q000378:metabolism; D021381:Protein Transport; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*
chemical_list: C534600:LecB protein, Pseudomonas aeruginosa; D037102:Lectins