title: Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture.
abstract: In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, 1-5Ka, Anam-Dong, Sungbuk-Ku, Seoul 136-701, Republic of Korea. drhyshin@korea.ac.kr
authors.forename: Hyun Yong
authors.initials: HY
authors.lastname: Shin
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Chulhwan
authors.initials: C
authors.lastname: Park
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000164:Acremonium / Q000254:growth & development* / Q000378:metabolism; D000591:Amino Acid Isomerases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis*; D003470:Culture Media / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D010453:Peptide Synthases / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D003470:Culture Media; C025163:cephalosporin C; D002244:Carbon; D008715:Methionine; D000591:Amino Acid Isomerases; C040886:isopenicillin N epimerase; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase; D003545:Cysteine; D005990:Glycerol