title: Canine C-reactive protein measurements in cerebrospinal fluid by a time-resolved immunofluorimetric assay.
abstract: In the current study, the quantification of C-reactive protein (CRP) in cerebrospinal fluid (CSF) of dogs using an adapted time-resolved immunofluorimetric assay (TR-IFMA) was investigated, as well as whether the assay could be used to detect the range of CRP concentrations found in different clinical situations. Intra- and interassay coefficients of variation were below 15% in all cases. The TR-IFMA measured the CRP values in a proportional and linear manner (r  =  0.99); also CRP concentrations measured in CSF and in serum were significantly correlated (r  =  0.80, P  =  0.003). The limit of detection of the method was 7.1 × 10(-6) mg/l. The assay was able to detect differences in CRP concentrations in CSF of dogs with inflammatory disorders compared with dogs with spinal cord compression or idiopathic epilepsy. In conclusion, TR-IFMA constitutes a very sensitive, precise, and accurate method for the measurement of CRP concentrations in CSF.
authors.affiliation: Animal Medicine and Surgery Department, Faculty of Veterinary Medicine, University of Murcia, 30100 Espinardo Campus, Murcia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Martínez-Subiela
authors.forename: Marco
authors.initials: M
authors.lastname: Caldin
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Parra
authors.forename: Nicola
authors.initials: N
authors.lastname: Ottolini
authors.forename: Giovanna
authors.initials: G
authors.lastname: Bertolini
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Bernal
authors.forename: Juan D
authors.initials: JD
authors.lastname: García-Martinez
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Cerón
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000134:cerebrospinal fluid*; D004283:Dog Diseases / Q000134:cerebrospinal fluid*; D004285:Dogs; D015200:Fluoroimmunoassay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D057230:Limit of Detection; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric
chemical_list: D002097:C-Reactive Protein