title: Read-through activation of transcription in a cellular genomic context.
abstract: Read-through transcription from the adjacent E1a gene region is required for wild-type (wt) activity of the downstream adenovirus E1b promoter early after infection (read-through activation). However, whether a cellular chromosomal template can support read-through activation is not known. To address this issue, read-through activation was evaluated in the context of stably expressed templates in transfected cells. Inhibition of read-through transcription by insertion of a transcription termination sequence between the E1a and E1b promoters reduced downstream gene expression from stably integrated templates. The results indicate that the mechanism of read-through activation does not depend on the structure of early adenovirus nucleoprotein complexes, a structure that is likely to be different from that of cellular chromatin. Accordingly, this regulatory interaction could participate in the coordinated control of the expression of closely linked cellular genes.
authors.affiliation: Department of Microbiology and Immunology, Inter-College Graduate Degree Program in Genetics, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania, United States of America.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: David J
authors.initials: DJ
authors.lastname: Spector
mesh_terms: D017500:Adenovirus E1A Proteins / Q000235:genetics*; D017501:Adenovirus E1B Proteins / Q000235:genetics*; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods; D017930:Genes, Reporter; D023281:Genomics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D017500:Adenovirus E1A Proteins; D017501:Adenovirus E1B Proteins; D002843:Chromatin; D049452:Green Fluorescent Proteins; D004247:DNA