title: The mutL mutation in Pseudomonas aeruginosa isolates reveals multidrug-resistant traits and possible evolutionary trends.
abstract: To evaluate the genetic differences and possible evolutionary trends of clinical multidrug-resistant (MDR) strains, Pseudomonas aeruginosa isolates were characterized by pulsed-field gel electrophoresis (PFGE) and evolutionary distances were estimated. A total of 85.7% of the P. aeruginosa isolates were MDR strains. Strains with the PFGE pattern A predominated; all were susceptible to amikacin and cefepime but resistant to levofloxacin and meropenem (except strain PA45 which was sensitive to meropenem). PFGE pattern H or P strains exhibited resistance to six to eight different antibiotics. PFGE pattern I or J strains were susceptible to all antibiotics tested. Two imperfect six base-pair tandem repeats, CTGGCG and CTGGCC, were found in the mutL gene. In conclusion, MDR characteristics and PFGE profiles were clearly correlated with the mutL phylogenetic tree. This indicates that mutations in mutL might contribute to genetic stability in adaptation by changing the MDR traits. Phylogenetic analysis of mutL revealed the MDR relatedness of P. aeruginosa strains.
authors.affiliation: Department of General Surgery, Peking University First Hospital, Beijing, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Wang
authors.forename: S L
authors.initials: SL
authors.lastname: Liu
authors.forename: G R
authors.initials: GR
authors.lastname: Liu
authors.forename: Y L
authors.initials: YL
authors.lastname: Wan
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: P Y
authors.initials: PY
authors.lastname: Wang
authors.forename: Q S
authors.initials: QS
authors.lastname: Wang
authors.forename: X L
authors.initials: XL
authors.lastname: Cao
authors.forename: Y C
authors.initials: YC
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D019143:Evolution, Molecular; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D019655:Quantitative Trait, Heritable
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins