title: [Expression of regulation protein of glial fibrillary acidic protein gene in hippocampus of epileptic rats induced by kainic acid].
abstract: OBJECTIVE
  The DNA binding protein from hippocampus of epileptic rats was investigated by southwestern blot, which regulated the expression of glial fibrillary acidic protein (GFAP) gene. Its expression pattern was also studied.
  
  
  RESULTS
  Southwestern blot showed that there were two specific DNA binding proteins that regulated the expression of GFAP gene, and their molecular weight were 39 kDa and 35.5 kDa, respectively. The expression of the DNA binding proteins was markedly increased in hippocampus of kainic acid (KA) + NS group at 5 d, reached the peak at 3 w, lasted for 3 m observed.
  
  
  CONCLUSIONS
  The results above suggest that KA may increase the expression of GFAP in hippocampus by upregulating the transcription factor that regulated the expression of GFAP gene, which may participate in the formation of epileptic seizures.
authors.affiliation: Department of Physiology, Dalian Medical University, Dalian 116027, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Jiang
authors.forename: M
authors.initials: M
authors.lastname: Huang
authors.forename: X F
authors.initials: XF
authors.lastname: An
authors.forename: W Q
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid / Q000009:adverse effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D007608:Kainic Acid