title: Regeneration of skull bones in adult rabbits after implantation of commercial osteoinductive materials and transplantation of a tissue-engineering construct.
abstract: We performed a comparative study of reparative osteogenesis in rabbits with experimental critical defects of the parietal bones after implantation of commercial osteoinductive materials "Biomatrix", "Osteomatrix", "BioOss" in combination with platelet-rich plasma and transplantation of a tissue-engineering construct on the basis of autogenic multipotent stromal cells from the adipose tissue predifferentiated in osteogenic direction. It was found that experimental reparative osteogenesis is insufficiently stimulated by implantation materials and full-thickness trepanation holes were not completely closed. After transplantation of the studied tissue-engineering construct, the defect was filled with full-length bone regenerate (in the center of the regenerate and from the maternal bone) in contrast to control and reference groups, where the bone tissue was formed only on the side of the maternal bone. On day 120 after transplantation of the tissue-engineering construct, the percent of newly-formed bone tissue in the regenerate was 24% (the total percent of bone tissue in the regenerate was 39%), which attested to active incomplete regenerative process in contrast to control and reference groups. Thus, the study demonstrated effective regeneration of the critical defects of the parietal bones in rabbits 120 days after transplantation of the tissue-engineering construct in contrast to commercial osteoplastic materials for directed bone regeneration.
authors.affiliation: Institute of Human Morphology, Russian Academy of Medical Sciences, Moscow, Russia. alex.volkoff@gmail.com
authors.forename: A V
authors.initials: AV
authors.lastname: Volkov
authors.forename: I S
authors.initials: IS
authors.lastname: Alekseeva
authors.forename: A A
authors.initials: AA
authors.lastname: Kulakov
authors.forename: D V
authors.initials: DV
authors.lastname: Gol'dshtein
authors.forename: S A
authors.initials: SA
authors.lastname: Shustrov
authors.forename: A I
authors.initials: AI
authors.lastname: Shuraev
authors.forename: I V
authors.initials: IV
authors.lastname: Arutyunyan
authors.forename: T B
authors.initials: TB
authors.lastname: Bukharova
authors.forename: A A
authors.initials: AA
authors.lastname: Rzhaninova
authors.forename: G B
authors.initials: GB
authors.lastname: Bol'shakova
authors.forename: A S
authors.initials: AS
authors.lastname: Grigor'yan
mesh_terms: D000818:Animals; D001857:Bone Matrix; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation; D005260:Female; D008297:Male; D008903:Minerals / Q000627:therapeutic use; D010012:Osteogenesis; D010294:Parietal Bone / Q000502:physiology*; D053657:Platelet-Rich Plasma / Q000502:physiology; D011817:Rabbits; D017154:Stromal Cells / Q000637:transplantation*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals