title: Proteome analysis of proteins related to aggressive periodontitis combined with neutrophil chemotaxis dysfunction.
abstract: OBJECTIVE
  Some patients suffering from aggressive periodontitis (Ag-P) also display neutrophil chemotaxis dysfunction. In this study, we attempted to identify the proteins involved in Ag-P associated with neutrophil chemotaxis dysfunction using proteome analysis.
  
  
  METHODS
  A two-dimensional fluorescence difference gel electrophoresis system was used to detect differences in protein expression between neutrophils from four patients suffering from Ag-P combined with neutrophil chemotaxis dysfunction and those from four controls. Moreover, the mRNA levels of the proteins identified by the above method were examined in neutrophils from four types of subjects using the real-time polymerase chain reaction: twenty patients suffering from Ag-P with or without the dysfunction, 15 patients with chronic periodontitis, and 15 controls.
  
  
  RESULTS
  Four proteins, lactoferrin, caldesmon, heat shock protein 70, and stac, displayed a higher protein expression level in the neutrophils from the patients suffering from Ag-P combined with the neutrophil dysfunction than in those from the control group. The caldesmon mRNA levels in the neutrophils from the patients suffering from Ag-P combined with the neutrophil dysfunction were high compared with those in the neutrophils from the patients suffering from the other two types of periodontitis and those from the control group.
  
  
  CONCLUSIONS
  Caldesmon may be a marker of Ag-P combined with neutrophil chemotaxis dysfunction.
authors.affiliation: Department of Periodontal Medicine, Division of Frontier Medical Science, Graduate School of Biomedical Sciences Research Facility, Faculty of Dentistry, Hiroshima University, Hiroshima, Japan. mizuno@hiroshima-u.ac.jp
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Mizuno
authors.forename: Miyuki
authors.initials: M
authors.lastname: Niitani
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiba
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Iwata
authors.forename: Ikue
authors.initials: I
authors.lastname: Hayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D016022:Case-Control Studies; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D055113:Chronic Periodontitis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005884:Gingival Hemorrhage / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D007781:Lactoferrin / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis; D009504:Neutrophils / Q000502:physiology*; D017622:Periodontal Attachment Loss / Q000378:metabolism; D010514:Periodontal Pocket / Q000378:metabolism; D010519:Periodontium / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D002148:Calmodulin-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D020543:Proteome; D012333:RNA, Messenger; C103302:STAC protein, human; D007781:Lactoferrin