title: Structural proteins of the primary cell wall: extraction, purification, and analysis.
abstract: Structural proteins of the primary cell wall present unusual but interesting problems for structural biologists in particular and plant biologists in general. As structure is the key to function; then the biochemical isolation of these glycoproteins for further study is paramount. Here, we detail the "classical" method for isolating soluble extensin monomers by elution of monomeric precursors to network extensin from tissue cultures. We also outline an additional approach involving genetic engineering that can potentially yield the complete genomic range of extensins and other hydroxyproline-rich glycoprotein (HRGPs) currently underutilized for biotechnology.
authors.affiliation: School of Life Sciences, University of Sussex, Falmer, Brighton, UK. derekt.t.a.lamport@googlemail.com
authors.forename: Derek T A
authors.initials: DT
authors.lastname: Lamport
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.forename: Marcia J
authors.initials: MJ
authors.lastname: Kieliszewski
mesh_terms: D018929:Cell Culture Techniques; D002473:Cell Wall / Q000737:chemistry*; D006023:Glycoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D009088:Mucoproteins / Q000096:biosynthesis / Q000302:isolation & purification*; D033903:Periplasmic Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D006023:Glycoproteins; D009088:Mucoproteins; D033903:Periplasmic Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C006619:arabinogalactan proteins; C005488:extensin protein, plant; D049452:Green Fluorescent Proteins