title: Molecular characterization of adenoviruses in fecal samples of captively bred rhesus macaques in China.
abstract: In an attempt to study the molecular diversity of simian adenoviruses in nonhuman primate (NHP) populations, we screened a colony of captively bred rhesus macaques (Macaca mulatta) in China for the presence of adenoviral DNA in stool samples. This was done by using the nested PCR method that targeted the adenovirus polymerase gene. Among the 57 animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA and the PCR fragments were cloned for sequencing and phylogenetic analyses. The results suggested that the viral DNA clones were primarily segregated into two large groups: SAdV-6 (2 non-redundant sequences) and SAdV-7 (9 non-redundant sequences). In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively. Our data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.
authors.affiliation: Vaccine Research Institute, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Lu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Guangping
authors.initials: G
authors.lastname: Gao
mesh_terms: D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000261:Adenoviruses, Simian / Q000145:classification* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002681:China; D004279:DNA, Viral / Q000235:genetics; D005243:Feces / Q000821:virology; D005260:Female; D008253:Macaca mulatta / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral