title: Effect of natural organic matter on toxicity and reactivity of nano-scale zero-valent iron.
abstract: Nano-scale zero-valent iron (NZVI) particles are increasingly used to remediate aquifers contaminated with hazardous oxidized pollutants such as trichloroethylene (TCE). However, the high reduction potential of NZVI can result in toxicity to indigenous bacteria and hinder their participation in the cleanup process. Here, we report on the mitigation of the bactericidal activity of NZVI towards gram-negative Escherichia coli and gram-positive Bacillus subtilis in the presence of Suwannee River humic acids (SRHA), which were used as a model for natural organic matter (NOM). B. subtilis was more tolerant to NZVI (1 g/L) than E. coli in aerobic bicarbonate-buffered medium. SRHA (10 mg/L) significantly mitigated toxicity, and survival rates after 4 h exposure increased to similar levels observed for controls not exposed to NZVI. TEM images showed that the surface of NZVI and E. coli was surrounded by a visible floccus. This decreased the zeta potential of NZVI from -30 to -45 mV and apparently exerted electrosteric hindrance to minimize direct contact with bacteria, which mitigated toxicity. H(2) production during anaerobic NZVI corrosion was not significantly hindered by SRHA (p > 0.05), However, NZVI reactivity towards TCE (20 mg/L), assessed by the first-order dechlorination rate coefficient, decreased by 23%. Overall, these results suggest that the presence of NOM offers a tradeoff for NZVI-based remediation, with higher potential for concurrent or sequential bioremediation at the expense of partially inhibited abiotic reactivity with the target contaminant (TCE).
authors.affiliation: State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing 100083, China. chenjiawei@cugb.edu.cn
authors.forename: Jiawei
authors.initials: J
authors.lastname: Chen
authors.forename: Zongming
authors.initials: Z
authors.lastname: Xiu
authors.forename: Gregory V
authors.initials: GV
authors.lastname: Lowry
authors.forename: Pedro J J
authors.initials: PJ
authors.lastname: Alvarez
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D001639:Bicarbonates / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006812:Humic Substances; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000633:toxicity*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009930:Organic Chemicals / Q000737:chemistry*; D045483:Rivers / Q000737:chemistry; D014241:Trichloroethylene / Q000737:chemistry / Q000378:metabolism; D018508:Water Purification / Q000379:methods
chemical_list: D001639:Bicarbonates; D006812:Humic Substances; D009930:Organic Chemicals; D014241:Trichloroethylene; D006859:Hydrogen; D007501:Iron