title: Preparation and immunological characterization of β-lactoglobulin-amylose conjugate.
abstract: β-Lactoglobulin (BLG), a major allergen of cow's milk, was conjugated with the N-hydroxysuccinimide ester of the amylose-glycylglycine adduct (AG-ONSu) to reduce its immunogenicity, and the biochemical and immunological properties of the resulting conjugate (AG-BLG) were studied. The conjugate was prepared by modifying BLG with AG-ONSu, and was purified in a Sephadex G-100 column. The analytical data for AG-BLG indicated that 10.5 moles of AG-ONSu, with a mean molecular weight of 2,800, was covalently attached to the amino groups of the BLG molecule. Conjugation with AG-ONSu greatly decreased the reactivity of BLG with anti-BLG polyclonal antibodies owing to its shielding action for epitopes on the protein's surface. These findings suggest that AG-ONSu can be used advantageously to suppress the hypersensitivity mediated by IgG antibodies in milk allergy.
authors.affiliation: Laboratory of Biochemistry, Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nodake
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fukumoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukasawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yamasaki
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Sakakibara
mesh_terms: D000688:Amylose / Q000737:chemistry*; D000818:Animals; D006033:Glycylglycine / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007782:Lactoglobulins / Q000737:chemistry* / Q000276:immunology*; D051379:Mice; D013388:Succinimides / Q000737:chemistry
chemical_list: D018796:Immunoconjugates; D007074:Immunoglobulin G; D007782:Lactoglobulins; D013388:Succinimides; D006033:Glycylglycine; C032620:succinimide; D000688:Amylose