title: [Gene transfer-induced human heme oxygenase-1 over-expression protects kidney from ischemia-reperfusion injury in rats].
abstract: OBJECTIVE
  To study the protection of gene transfer-induced human heme oxygenase-1 over-expression against renal ischemia reperfusion injury in rats.
  
  
  METHODS
  The model of kidney ischemia-reperfusion injury was established with Sprague-Dawley rats. In the therapy group (n=18), the left kidney was perfused and preserved with Ad-hHO-1 at 2.5×10(9) pfu/1.0 ml after flushed with 0-4°C HC-A organ storage solution via donor renal aorta. The rats in control groups were perfused with 0.9% saline solution (n=12) or the vector carrying no interest gene Ad-EGFP 2.5×10(9) pfu/1.0 ml (n=18) instead of Ad-hHO-1. BUN and Cr in serum were measured by slide chemical methods. The kidney samples of rats were harvested for assay of histology, immunohistochemistry and quantification of HO enzymatic activity. Apoptosis cells in the kidney were measured by TUNEL.
  
  
  RESULTS
  Ad-hHO-1 via donor renal aorta could transfect renal cells of rats effectively, enzymatic activity of HO in treated group [(1.62±0.07) nmol×mg(-1)×min(-1)] is higher than in control groups treated with saline solution team [(1.27±0.07) nmol×mg(-1)×min(-1)] and vector EGFP team [(1.22±0.06) nmol×mg(-1)×min(-1)] (P<0.01). Immunohistochemically, we found that the rats treated with Ad-hHO-1 expressed hHO-1 in kidneys at a high level. Corresponding to this, the level of BUN and Cr, as well as the number of apoptosis cells, were decreased, and the damage in histology by HE staining was ameliorated.
  
  
  CONCLUSIONS
  Over-expression of human HO-1 can protect the kidney from ischemia/reperfusion injury in rats.
authors.affiliation: Department of Urology, First Hospital Affiliated to Suzhou University, Suzhou 215006, China.
authors.forename: Jin-xing
authors.initials: JX
authors.lastname: Lü
authors.forename: Chun-yin
authors.initials: CY
authors.lastname: Yan
authors.forename: Jin-xian
authors.initials: JX
authors.lastname: Pu
authors.forename: Jian-quan
authors.initials: JQ
authors.lastname: Hou
authors.forename: He-xing
authors.initials: HX
authors.lastname: Yuan
authors.forename: Ji-gen
authors.initials: JG
authors.lastname: Ping
mesh_terms: D000818:Animals; D015870:Gene Expression; D005822:Genetic Vectors; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000473:pathology*; D016030:Kidney Transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000628:therapy*; D014162:Transfection
chemical_list: C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1