title: Structural characterization of intracellular C-terminal domains of group III metabotropic glutamate receptors.
abstract: Metabotropic glutamate receptors (mGluRs) are regulated by interacting proteins that mostly bind to their intracellular C-termini. Here, we investigated if mGluR6, mGluR7a and mGluR8a C-termini form predefined binding surfaces or if they were rather unstructured. Limited tryptic digest of purified peptides argued against the formation of stable globular folds. Circular dichroism, (1)H NMR and (1)H(15)N HSQC spectra indicated the absence of rigid secondary structure elements. Furthermore, we localized short linear binding motifs in the unstructured receptor domains. Our data provide evidence that protein interactions of the analyzed mGluR C-termini are mediated rather by short linear motifs than by preformed folds.
authors.affiliation: Institut für Biochemie (Emil-Fischer-Zentrum), Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Seebahn
authors.forename: Holger
authors.initials: H
authors.lastname: Dinkel
authors.forename: Jeannine
authors.initials: J
authors.lastname: Mohrlüder
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hartmann
authors.forename: Nico
authors.initials: N
authors.lastname: Vogel
authors.forename: Cord-Michael
authors.initials: CM
authors.lastname: Becker
authors.forename: Heinrich
authors.initials: H
authors.lastname: Sticht
authors.forename: Ralf
authors.initials: R
authors.lastname: Enz
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002942:Circular Dichroism; D019295:Computational Biology / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D011492:Protein Hydrolysates / Q000737:chemistry; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary; D051381:Rats; D018094:Receptors, Metabotropic Glutamate / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010446:Peptide Fragments; D011492:Protein Hydrolysates; D020033:Protein Isoforms; D018094:Receptors, Metabotropic Glutamate; D011993:Recombinant Fusion Proteins; C113897:metabotropic glutamate receptor 6; C102719:metabotropic glutamate receptor 7; C111435:metabotropic glutamate receptor 8