title: Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27.
abstract: In this study, individual Japanese flounder were intraperitoneally injected with 2 μg tributyltin-d27 (TBT-d27) fish⁻¹. Blood samples were collected on day 7 after injection. TBT-binding protein types 1 and 2 (TBT-bp1, -bp2) in the blood serum were quantified by western blotting analysis. As a result, the concentration of TBT-bp2 in TBT-d27 treated group increased to 220% of that in the solvent control, whereas the TBT-bp1 concentration decreased to 65% of that in the solvent control. Additionally, a positive relationship between the concentrations of TBT-bp2 and TBT was observed in blood sera of wild and cultured flounder. We suggest that TBT-bp2 is produced in response to TBT exposure and may play an important role in fish physiology.
authors.affiliation: Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka, Japan.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Nassef
authors.forename: Takahiko
authors.initials: T
authors.lastname: Tawaratsumita
authors.forename: Yumi
authors.initials: Y
authors.lastname: Oba
authors.forename: Hina
authors.initials: H
authors.lastname: Satone
authors.forename: Kei
authors.initials: K
authors.lastname: Nakayama
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Honjo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000008:administration & dosage / Q000633:toxicity*; D029941:Fish Proteins / Q000097:blood*; D005432:Flounder / Q000097:blood* / Q000502:physiology; D014220:Trialkyltin Compounds / Q000008:administration & dosage / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D002352:Carrier Proteins; D052244:Endocrine Disruptors; D029941:Fish Proteins; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin