title: Bioautography detection in thin-layer chromatography.
abstract: Bioautography is a microbial detection method hyphenated with planar chromatography techniques. It is based mainly on antimicrobial or antifungal properties of analyzed substances. The review discusses three versions of bioautography, i.e. contact, immersion and direct bioautography. The more concern is given to the last one. Many applications are quoted, not only for testing various groups of compounds, but also for investigating biochemical processes and factors influencing bacterial growth. Additionally, related methods, which can be included into direct bioautography, are discussed. The most promising among them seems to be TLC-bioluminescence screening.
authors.affiliation: Department of Chromatographic Methods, University of Maria Curie-Skłodowska, M. Skłodowska Sq. 3, 20-031 Lublin, Poland. irena.choma@umcs.lublin.pl
authors.forename: Irena M
authors.initials: IM
authors.lastname: Choma
authors.forename: Edyta M
authors.initials: EM
authors.lastname: Grzelak
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002855:Chromatography, Thin Layer / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts