title: [The role of LFA-1 in the vascular endothelial cells injury mediated by frozen/thawed neutrophils].
abstract: OBJECTIVE
  To investigate the mechanism of the vascular endothelial cell (VEC) injury caused by freezing/thawing.
  
  
  METHODS
  The frozen/thawed neutrophil (PMN) model was founded by freezing PMNs with a rate cooling instrument and then rewarming them in a water bath, the PMNs used here were separated from rat's peripheral blood using density gradients centrifugation techniques. The expression of LFA-1 on the surface of frozen/thawed PMNs was observed at 4 h,12 h and 24 h after freezing/thawing. After co-incubating untreated VECs with frozen/thawed PMNs, we detected the VEC injury and the changes in PMN-VEC adhesion.
  
  
  RESULTS
  (1) The PMNs LFA-1 expression increased in a time-dependent manner within 24 h after the freezing/thawing of PMNs. (2) After co-incubating untreated VECs with frozen/thawed PMNs, the adhesion between frozen/thawed PMNs and VECs increased and VEC injury occurred. (3) Monoclonal antibody against LFA-1 could block the PMN-VEC adhesion and subsequently attenuated the VEC injury.
  
  
  CONCLUSIONS
  The freezing/thawing of PMNs can elicited an increase in PMN LFA-1 expression and trigger the PMN-VEC adhesion and subsequently bring about the VEC injury.
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Liu
authors.forename: Zeng-Ren
authors.initials: ZR
authors.lastname: Yang
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D005615:Freezing; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1