title: Distribution, origin and contamination risk of coagulase-negative staphylococci from platelet concentrates.
abstract: Transfusion-associated bacterial sepsis is the most common microbiological risk of transfusion and is caused mostly by platelet concentrates (PCs). The most frequently identified bacterial contaminants of PCs are coagulase-negative staphylococci (CNS). In order to learn more about the distribution, source and risk of the CNS that are involved in bacterial contamination of PCs, CNS strains isolated during platelet screening were collected and characterized to the species level with three different methods: 16S rRNA and sodA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and amplified fragment length polymorphism (AFLP) analysis. AFLP analysis was also used for the typing of the CNS strains. A total of 83 CNS strains were analysed by sequencing and 8 different CNS species were identified, with Staphylococcus epidermidis being the predominant species. MALDI-TOF MS and AFLP analysis confirmed these results to a large extent. However, MALDI_TOF MS could not identify all strains to the species level and AFLP analysis revealed an additional, likely novel, CNS species. The species identified are mainly recognized as being part of the normal skin flora. Typing of the CNS strains by AFLP analysis showed that there was not a unique strain which is significantly more often present during bacterial contamination of PCs.
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.|Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands.
authors.forename: Ineke G H
authors.initials: IGH
authors.lastname: Rood
authors.affiliation: Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands.
authors.forename: Dirk
authors.initials: D
authors.lastname: de Korte
authors.affiliation: Canadian Blood Services, Ottawa, Ontario, Canada.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ramírez-Arcos
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Paul H M
authors.initials: PHM
authors.lastname: Savelkoul
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Annika
authors.initials: A
authors.lastname: Pettersson
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001792:Blood Platelets / Q000382:microbiology*; D003030:Coagulase / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D009426:Netherlands; D017713:Platelet Transfusion / Q000009:adverse effects*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018805:Sepsis / Q000097:blood / Q000382:microbiology / Q000635:transmission*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013203:Staphylococcal Infections / Q000097:blood / Q000382:microbiology / Q000635:transmission*; D013210:Staphylococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D013212:Staphylococcus epidermidis / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003030:Coagulase; D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase