title: Transcriptome analysis of an antibiotic downregulator mutant and synergistic Actinorhodin stimulation via disruption of a precursor flux regulator in Streptomyces coelicolor.
abstract: Through microarray analysis of an antibiotic-downregulator-deleted Streptomyces coelicolor ΔwblA ΔSCO1712 mutant, 28 wblA- and SCO1712-dependent genes were identified and characterized. Among 14 wblA- and SCO1712-independent genes, a carbon flux regulating 6-phosphofructokinase SCO5426 was additionally disrupted in the ΔwblA ΔSCO1712 mutant and further stimulated actinorhodin production in S. coelicolor, implying that both regulatory and precursor flux pathways could be synergistically optimized for antibiotic production.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 402-751, South Korea.
authors.forename: Seon-Hye
authors.initials: SH
authors.lastname: Kim
authors.forename: Han-Na
authors.initials: HN
authors.lastname: Lee
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Eung-Soo
authors.initials: ES
authors.lastname: Kim
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics; D046228:Microarray Analysis; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D002244:Carbon; C013390:actinorhodin; D000095622:Benzoisochromanequinones