title: Hsp90β1 knockout targeted to male germline: a mouse model for globozoospermia.
abstract: To study the role of Hsp90β1, an endoplasmic chaperone, we have built a conditional knockout by crossing Hsp90β1(flox/flox) with the Vasa-Cre transgenic line. Spermatozoa deficient in Hsp90β1 could not naturally fertilize oocytes and exhibited large and globular heads with abnormal intermediate pieces, a phenotype reminiscent of human globozoospermia.
authors.affiliation: Centre de Biologie du Développement, Université Toulouse III, Toulouse, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Audouard
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Christians
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018390:Gene Targeting / Q000379:methods; D018841:HSP90 Heat-Shock Proteins / Q000172:deficiency* / Q000235:genetics; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011247:Pregnancy; D013094:Spermatozoa / Q000002:abnormalities*
chemical_list: D018841:HSP90 Heat-Shock Proteins; C490097:Hsp90b1 protein, mouse