title: [Effect of astragalus polysaccharide on the function and maturation of plasmacytoid dendritic cells from chronic myelogenous leukemia before and after treatment].
abstract: OBJECTIVE
  To explore the effect of astragalus polysaccharide (APS) on the function and maturation of chronic myelogenous leukemia (CML) peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs).
  
  
  METHODS
  CML-derived pDCs were sorted by flow cytometry, and then incubated with APS (at 0, 50, 100 and 200 mg/L). After 24 hours, the concentrations of IFN-α, IL-6, TNF-α were detected with ELISA. Five days later, the cultured cells were collected and analyzed for immotype, morphology and ultramicrostructure.
  
  
  RESULTS
  The level of IFN-α, IL-6, TNF-α was significantly higher in samples from CML remission group than that in untreated pDCs, and newly diagnosed pDC (P < 0.05) or untreated group. APS could promote more pDCs differentiating to dendritic cells (DCs) in CML remission group than in untreated-pDCs in a dose-dependant manner (P < 0.05).
  
  
  CONCLUSIONS
  APS can enhance the immune function of pDCs, promote differentiation and maturation of pDCs from CML patients.
authors.affiliation: Department of Hematology, Second People Hospital of Lanzhou University, Lanzhou 730030, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Liu
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Zhang
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology; D007963:Leukocytes, Mononuclear; D011134:Polysaccharides / Q000494:pharmacology
chemical_list: D016898:Interferon-alpha; D011134:Polysaccharides