title: Troponin I degradation in serum of patients with acute ischemic stroke.
abstract: BACKGROUND
  Although troponin is a cornerstone biomarker in the assessment and management of patients with acute coronary syndrome, much remains to be learned about the biology of this widely used biomarker, including its post-release modification. Degradation of troponin following release in patients with acute coronary syndrome has been described; however whether such post-release modification occurs in other non-acute coronary syndrome states remains unknown. The aim of this study was to define troponin degradation in patients with acute ischemic stroke.
  
  
  METHODS
  Troponin I (cTnI) was measured daily during the first 5 days of admission in 244 patients with acute ischemic stroke. Western blot analysis was performed using anti-cTnI antibodies and compared with serum concentrations of cTnI in seven patients and one patient with myocardial infarction (positive control).
  
  
  RESULTS
  Elevated levels of troponin were detected in 25 (10%) patients; in all, both intact cTnI and cTnI degradation products were detected, with up to seven degradation fragments found. Samples with the highest total cTnI levels gave the strongest and most numerous western-blotting bands. All fragments were comparable with the degradation pattern of the positive control in terms of position.
  
  
  CONCLUSIONS
  Immunoblotting of blood samples from patients with acute ischemic stroke reveals similar degradation patterns of cTnI as has been described in patients with acute myocardial infarction. The biological ramification and potential clinical impact of this finding bears further scrutiny.
authors.affiliation: Department of Cardiology, Odense University Hospital, Denmark. jesperkjensen@dadlnet.dk
authors.forename: Jesper K
authors.initials: JK
authors.lastname: Jensen
authors.forename: Jonas
authors.initials: J
authors.lastname: Hallén
authors.forename: Terje
authors.initials: T
authors.lastname: Lund
authors.forename: Lene Helleskov
authors.initials: LH
authors.lastname: Madsen
authors.forename: Zanina
authors.initials: Z
authors.lastname: Grieg
authors.forename: James L
authors.initials: JL
authors.lastname: Januzzi
authors.forename: Dan
authors.initials: D
authors.lastname: Atar
mesh_terms: D000368:Aged; D015153:Blotting, Western; D005260:Female; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000097:blood* / Q000150:complications*; D011499:Protein Processing, Post-Translational; D020521:Stroke / Q000097:blood* / Q000150:complications*; D013997:Time Factors; D019210:Troponin I / Q000097:blood*
chemical_list: D019210:Troponin I