title: Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch).
abstract: The present investigation was undertaken to study the induction of DNA damage by lead chloride (PbCl(2)) in freshwater climbing perch Anabas testudineus using alkaline single cell gel electrophoresis (comet assay). Based on the LC(50) values of lead chloride of A. testudineus three different concentrations viz., 0.1, 1.0 and 2.0 mg/L were selected to expose fish. The DNA damage was observed in the gill, kidney and liver tissue as the percentage of DNA in comet tails and comet heads in the tissue of the exposed fish. DNA damage at different concentrations showed sensitivity to particular tissue. The liver tissue exhibited significantly (p < 0.01) higher DNA damage, followed by kidney and gill. However, the DNA damage was found to be dose dependent; at 2 mg/L of PbCl(2) the tail and head DNA of liver tissue were 57.84% and 39.49%, in kidney tissue the values were 52.36% and 44.97% whereas in gill tissue the values were 48.86% and 48.96% respectively. The current study explored the utility of the comet assay for in vivo laboratory studies using A. testudineus species for screening the genotoxic potential of lead chloride.
authors.affiliation: Department of Fisheries, University of Dhaka, Dhaka 1000, Bangladesh. kawser@univdhaka.edu
authors.forename: Md Kawser
authors.initials: MK
authors.lastname: Ahmed
authors.forename: Elora
authors.initials: E
authors.lastname: Parvin
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arif
authors.forename: Md Monirul
authors.initials: MM
authors.lastname: Islam
authors.forename: Mosammat Salma
authors.initials: MS
authors.lastname: Akter
authors.forename: Mohammad Shahneawz
authors.initials: MS
authors.lastname: Khan
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water / Q000737:chemistry; D005880:Gills / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D007854:Lead / Q000633:toxicity*; D008099:Liver / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D010471:Perches / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical; D007854:Lead; C029891:lead chloride