title: SIGN-R1+MHC II+ cells of the splenic marginal zone--a novel type of resident dendritic cells.
abstract: In the spleen, the MZ forms an interface between red and white pulp. Its major function is to trap blood-borne antigens and to reorient them to APCs and lymphocytes. SIGN-R1(+) cells are of the MZ inherent cell population, which for a long time, have been considered as macrophages. We now show that one subpopulation of SIGN-R1(+) cells that express MHC II molecules should be considered as a resident DC. Histological analysis indicated that SIGN-R1(+) cells have dendritic-like protrusions extending into T and B cell areas. Flow cytometry analysis revealed an expression profile of adhesion, costimulatory, and MHC molecules similar to cDCs but distinct from macrophages. Most importantly, SIGN-R1(+)MHC(+) cells were able to present antigen to naïve CD4 T cells, as well as to cross-present soluble, particulate antigens secreted by Listeria monocytogenes to CD8 T cells in vitro and in vivo. Our experiments identified SIGN-R1(+)MHC II(+) cells as professional APCs and indicate their nature as splenic resident DCs.
authors.affiliation: Hannover Medical School, Institute for Immunology, Carl-Neuberg-Str. 1 (OE5240), Hannover, Lower Saxony D-30625, Germany. marcin.lyszkiewicz@gmail.com
authors.forename: Marcin
authors.initials: M
authors.lastname: Łyszkiewicz
authors.forename: Natalia
authors.initials: N
authors.lastname: Zietara
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.forename: Nelson O
authors.initials: NO
authors.lastname: Gekara
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Jabłońska
authors.forename: Kurt E
authors.initials: KE
authors.lastname: Dittmar
authors.forename: Siegfried
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015815:Cell Adhesion Molecules / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005434:Flow Cytometry; D005802:Genes, MHC Class II; D037181:Lectins, C-Type / Q000276:immunology*; D008089:Listeria monocytogenes / Q000472:pathogenicity; D008088:Listeriosis / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000276:immunology*; D013154:Spleen / Q000276:immunology* / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin