title: Canine specific ELISA for coagulation factor VII.
abstract: Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.
authors.affiliation: Department of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Copenhagen DK-1870, Denmark. tmkn@novonordisk.com
authors.forename: Tom
authors.initials: T
authors.lastname: Knudsen
authors.forename: Mads
authors.initials: M
authors.lastname: Kjelgaard-Hansen
authors.forename: Mikael
authors.initials: M
authors.lastname: Tranholm
authors.forename: Bo
authors.initials: B
authors.lastname: Wiinberg
authors.forename: Jes T
authors.initials: JT
authors.lastname: Clausen
authors.forename: Jens Jacob
authors.initials: JJ
authors.lastname: Hansen
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Nichols
authors.forename: Marianne
authors.initials: M
authors.lastname: Kjalke
authors.forename: Asger L
authors.initials: AL
authors.lastname: Jensen
authors.forename: Annemarie T
authors.initials: AT
authors.lastname: Kristensen
mesh_terms: D000818:Animals; D000941:Antigens / Q000097:blood*; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005167:Factor VII; D005168:Factor VII Deficiency / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D051379:Mice; D015203:Reproducibility of Results
chemical_list: D000941:Antigens; C031500:factor VII related antigen; D005167:Factor VII