title: Molecular characterization of Campylobacter lanienae strains isolated from food-producing animals.
abstract: During 2008 and 2009, within the framework of the Hungarian monitoring program of antibiotic resistance of zoonotic agents from food-producing animals, a significant number (43 strains) of Campylobacter lanienae were detected for the first time in Hungary. The isolates were genotyped using partial 16S rRNA gene sequencing and pulsed-field gel electrophoresis using three different restriction enzymes. The antimicrobial resistance of the isolates was determined by microtiter broth dilution. C. lanienae isolation was successful only from swine but not from other animal species. According to phylogenetic analysis, clustering of the isolates shows the same extensive genetic diversity as other Campylobacter species. Sequence analysis of the partial 16S rRNA gene showed that additional variations exist in variable regions Vc2 and Vc6. SmaI restriction enzyme proved to be the most efficient for pulsed-field gel electrophoresis analysis of C. lanienae. A significant tetracycline resistance (60.9%) and the presence of erythromycin-, enrofloxacin-, and multiresistant C. lanienae strains were found. Although the pathogenic potential of C. lanienae in humans is currently unknown, this study demonstrates that C. lanieanae is common in pigs in the country, provides further details on the genotypic and phenotypic properties of C. lanienae, and offers a genotyping method for use in source tracing.
authors.affiliation: Department of Bacteriology and Molecular Biology, Central Agricultural Office Veterinary Diagnostic Directorate, Tábornok u. 2, Budapest, Hungary. schweitzer.nora@gmail.com
authors.forename: Nóra
authors.initials: N
authors.lastname: Schweitzer
authors.forename: Ivelina
authors.initials: I
authors.lastname: Damjanova
authors.forename: Eva
authors.initials: E
authors.lastname: Kaszanyitzky
authors.forename: Krisztina
authors.initials: K
authors.lastname: Ursu
authors.forename: Péterné
authors.initials: P
authors.lastname: Samu
authors.forename: Adám György
authors.initials: AG
authors.lastname: Tóth
authors.forename: János
authors.initials: J
authors.lastname: Varga
authors.forename: Adám
authors.initials: A
authors.lastname: Dán
mesh_terms: D000003:Abattoirs; D000818:Animals; D001483:Base Sequence; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D006814:Hungary; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014422:Turkeys
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S