title: State-dependent disruption of short-term facilitation due to overexpression of the apPDE4 supershort form in Aplysia.
abstract: Phosphodiesterases (PDEs) play important roles in synaptic plasticity by regulating cAMP signaling in various organisms. The supershort, short, and long forms of Aplysia PDE4 (apPDE4) have been cloned, and the long form has been shown to play a crucial role in 5- hydroxytryptamine (5-HT)-induced synaptic plasticity in Aplysia. To address the role of the supershort form in 5-HT-induced synaptic plasticity in Aplysia, we overexpressed the apPDE4 supershort form in Aplysia sensory neurons. Consequently, 5-HT-induced hyperexcitability and short-term facilitation in nondepressed synapses were blocked. However, the supershort form did not inhibit 5-HT-induced short-term facilitation in highly depressed synapses. These results show that the supershort form plays an important role in 5-HT-induced synaptic plasticity and disrupts it mainly by impairing cAMP signaling in Aplysia.
authors.affiliation: National Creative Research Initiative Center for Memory, College of Natural Sciences, Seoul National University, Seoul, Korea.
authors.forename: Deok-Jin
authors.initials: DJ
authors.lastname: Jang
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Lee
authors.forename: Yeon-Su
authors.initials: YS
authors.lastname: Chae
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
mesh_terms: D000818:Animals; D001048:Aplysia / Q000187:drug effects / Q000201:enzymology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000201:enzymology; D012701:Serotonin / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D012701:Serotonin; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4