title: The antinociceptive and anti-inflammatory action of the CHCl3-soluble phase and its main active component, damnacanthal, isolated from the root of Morinda citrifolia.
abstract: Morinda citrifolia (Rubiaceae, Noni) is a traditional medicine with various pharmacological activities. We investigated if the MeOH-, CHCl(3)- and BuOH-soluble phase and its main active component, damnacanthal, isolated from the Noni root, have antinociceptive and anti-inflammatory actions in mice. The CHCl(3)-soluble phase (3 g/kg, per os (p.o.)) significantly reduced pain-related behavior observed in the formalin test. These effects were not suppressed by pretreatment with naloxone (1 mg/kg, intraperitoneally (i.p.)), an opioid receptor antagonist. The CHCl(3)-soluble phase (3 g/kg, p.o.) significantly reduced histamine-induced paw edema. The MeOH- and BuOH-soluble phase had no effect in either test. Furthermore, damnacanthal (10-100 mg/kg, p.o.) exerted an antinociceptive effect on chemical nociceptive stimuli, and decreased histamine-induced paw edema. Damnacanthal was weakly bound to the histamine H(1) receptor. These data suggest that the CHCl(3)-soluble phase of the Noni root has antinociceptive and anti-inflammatory effects. Furthermore, these effects of damnacanthal isolated from the Noni root is mediated in part by the histamine H(1) receptor.
authors.affiliation: Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, Hyogo 650–8586, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Okusada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakamoto
authors.forename: Mikako
authors.initials: M
authors.lastname: Nishida
authors.forename: Wakako
authors.initials: W
authors.lastname: Fujita-Hamabe
authors.forename: Kohei
authors.initials: K
authors.lastname: Kamiya
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Mizushina
authors.forename: Toshiko
authors.initials: T
authors.lastname: Satake
authors.forename: Shogo
authors.initials: S
authors.lastname: Tokuyama
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology; D000440:Butanols / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008815:Mice, Inbred Strains; D015394:Molecular Structure; D032066:Morinda / Q000737:chemistry*; D010146:Pain / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000880:Anthraquinones; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000440:Butanols; D010936:Plant Extracts; C079170:damnacanthal; D002725:Chloroform; D000432:Methanol