title: Different CSF β-amyloid processing in Alzheimer's and Creutzfeldt-Jakob disease.
abstract: Decreased levels of β-amyloid (Aβ) 1-42 in cerebrospinal fluid (CSF) are characteristic for Alzheimer's disease (AD) and are also evident in Creutzfeldt-Jakob disease (CJD). Aβ plaques are thought to be responsible for this decrease in AD patients, whereas such Aβ plaques are rarely seen in CJD. To investigate the Aβ pattern in brain and CSF of neuropathologically confirmed CJD and AD patients we used an electrophoretic method to investigate Aβ peptide fractions which are not accessible to ELISA and immunohistochemistry. We analyzed Aβ peptides in the CSF of autopsy-confirmed CJD and AD patients and the corresponding brain homogenates using a quantitative urea-based Aβ electrophoresis immunoblot (Aβ-SDS-PAGE/immunoblot).The CSF Aβ1-42 decrease correlated with the brain Aβ load in AD, but not in CJD. There was no difference in the soluble fractions of brain homogenate in AD and CJD. We therefore conclude that different mechanisms in AD and CJD are responsible for the Aβ1-42 decrease in the CSF.
authors.affiliation: Paracelsus-Elena Klinik, Kassel and Georg-August University, Göttingen, Germany. brit.mollenhauer@pk-mx.de
authors.forename: Brit
authors.initials: B
authors.lastname: Mollenhauer
authors.forename: Hermann
authors.initials: H
authors.lastname: Esselmann
authors.forename: Sigrun
authors.initials: S
authors.lastname: Roeber
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Schulz-Schaeffer
authors.forename: Claudia
authors.initials: C
authors.lastname: Trenkwalder
authors.forename: Mirko
authors.initials: M
authors.lastname: Bibl
authors.forename: Petra
authors.initials: P
authors.lastname: Steinacker
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kretzschmar
authors.forename: Jens
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Markus
authors.initials: M
authors.lastname: Otto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D001921:Brain / Q000378:metabolism; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000473:pathology; D005260:Female; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000134:cerebrospinal fluid*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)