title: 1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase.
abstract: MazF proteins are ribonucleases that cleave mRNA with high sequence-specificity as part of bacterial stress response and that are neutralized by the action of the corresponding antitoxin MazE. Prolonged activation of the toxin MazF leads to cell death. Several mazEF modules from gram-negative bacteria have been characterized in terms of catalytic activity, auto-regulation mechanism and structure, but less is known about their distant relatives found in gram-positive organisms. Currently, no solution NMR structure is available for any wild-type MazF toxin. Here we report the (1)H, (15)N and (13)C backbone and side-chain chemical shift assignments of this toxin from the pathogen bacterium Staphylococcus aureus. The BMRB accession number is 17288.
authors.affiliation: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium.
authors.forename: Valentina
authors.initials: V
authors.lastname: Zorzini
authors.forename: Sarah
authors.initials: S
authors.lastname: Haesaerts
authors.forename: Ambrose
authors.initials: A
authors.lastname: Cheung
authors.forename: Remy
authors.initials: R
authors.lastname: Loris
authors.forename: Nico A J
authors.initials: NA
authors.lastname: van Nuland
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D002247:Carbon Isotopes; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011994:Recombinant Proteins / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry*; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002247:Carbon Isotopes; C540055:MazE protein, Staphylococcus aureus; D009587:Nitrogen Isotopes; D011994:Recombinant Proteins; D012260:Ribonucleases