title: Use of cloud point extraction with derivatizing reagent for the extraction and determination of isoniazid.
abstract: A simple and sensitive cloud point extraction high-performance liquid chromatography method is proposed for the determination of isoniazid in blood. The procedure is based on the product of the reaction of isoniazid with benzaldehyde. It can be validated that there is a linear relationship between the signal of isonicotinyl hydrazone and the concentration of isoniazid. A cloud point extraction system of nonionic surfactant Triton X-100 is applied for preconcentration of isonicotinyl hydrazone. Then the analytes in surfactant-rich phase are detected with HPLC-UV system. calibration graph was obtained in the range of 2.0 × 10(-3)-0.5 mg/L, the detection limit was 5.0 × 10(-4) mg/L. Method validation is performed on serum samples spiked at two levels, the recoveries ranging from 82.17-83.81%, with relative standard deviations from 2.45% to 3.89%.
authors.affiliation: College of Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Chen
authors.forename: Zhi-ming
authors.initials: ZM
authors.lastname: Zhou
authors.forename: Ming-min
authors.initials: MM
authors.lastname: Yang
mesh_terms: D001547:Benzaldehydes / Q000737:chemistry; D002138:Calibration; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007538:Isoniazid / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D016014:Linear Models; D017830:Octoxynol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D012965:Sodium Chloride; D017830:Octoxynol; C032175:benzaldehyde; D007538:Isoniazid