title: The diversity and molecular modelling analysis of B₁₂ and B₁₂-independent glycerol dehydratases.
abstract: To broaden our knowledge on the diversity of glycerol dehydratases, comprehensive sequence and molecular modelling analyses of these enzymes were performed. Our sequence analysis showed that B₁₂-dependent and B₁₂-independent glycerol dehydratases are not related, suggesting that they evolved from different ancestors. Second, our study demonstrated that a gene fusion event occurred between α and β subunits of B₁₂-dependent glycerol dehydratases in several bacteria during enzyme evolution. In addition, our sequence and molecular modelling analyses revealed more B₁₂-independent glycerol dehydratases including hypothetical proteins. Furthermore, we found that some microorganisms contain both B₁₂-dependent and B₁₂-independent glycerol dehydratases in their genomes.
authors.affiliation: Department of Chemistry, University of Louisiana at Lafayette, Lafayette 70504, USA. yx13987@louisiana.edu
authors.forename: Yuemin
authors.initials: Y
authors.lastname: Liu
authors.forename: August A
authors.initials: AA
authors.lastname: Gallo
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Bajpai
authors.forename: Andrei
authors.initials: A
authors.lastname: Chistoserdov
authors.forename: Andrew
authors.initials: A
authors.lastname: Nelson
authors.forename: Leah
authors.initials: L
authors.lastname: Segura
authors.forename: Wu
authors.initials: W
authors.lastname: Xu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D003038:Cobamides / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D006836:Hydro-Lyases / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D008958:Models, Molecular; D020539:Sequence Analysis, Protein
chemical_list: D001426:Bacterial Proteins; D003038:Cobamides; D006836:Hydro-Lyases; C020671:glycerol dehydratase; C000913:cobamamide