title: Post-translational His-Cys cross-linkage formation in tyrosinase induced by copper(II)-peroxo species.
abstract: Autocatalytic formation of His-Cys cross-linkage in the enzyme active site of tyrosinase from Aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with Cu(II) under aerobic conditions, where a (μ-η(2):η(2)-peroxo)dicopper(II) species has been suggested to be involved as a key reactive intermediate.
authors.affiliation: Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Fujieda
authors.forename: Takuya
authors.initials: T
authors.lastname: Ikeda
authors.forename: Michiaki
authors.initials: M
authors.lastname: Murata
authors.forename: Sachiko
authors.initials: S
authors.lastname: Yanagisawa
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Aono
authors.forename: Kei
authors.initials: K
authors.lastname: Ohkubo
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagao
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogura
authors.forename: Shun
authors.initials: S
authors.lastname: Hirota
authors.forename: Shunichi
authors.initials: S
authors.lastname: Fukuzumi
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Shinobu
authors.initials: S
authors.lastname: Itoh
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology; D055162:Biocatalysis; D003300:Copper / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D003545:Cysteine / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000737:chemistry* / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational / Q000187:drug effects*
chemical_list: D003432:Cross-Linking Reagents; D009942:Organometallic Compounds; D006639:Histidine; D003300:Copper; D014442:Monophenol Monooxygenase; D003545:Cysteine