title: ATP-sensitive cation-channel in wheat (Triticum durum Desf.): identification and characterization of a plant mitochondrial channel by patch-clamp.
abstract: Indirect evidence points to the presence of K(+) channels in plant mitochondria. In the present study, we report the results of the first patch clamp experiments on plant mitochondria. Single-channel recordings in 150 mM potassium gluconate have allowed the biophysical characterization of a channel with a conductance of 150 pS in the inner mitochondrial membrane of mitoplasts obtained from wheat (Triticum durum Desf.). The channel displayed sharp voltage sensitivity, permeability to potassium and cation selectivity. ATP in the mM concentration range completely abolished the activity. We discuss the possible molecular identity of the channel and its possible role in the defence mechanisms against oxidative stress in plants.
authors.affiliation: Department of Experimental Biomedical Sciences, CNR Institute of Neuroscience, University of Padova, Padova, Italy. umberto.demarchi@unige.ch
authors.forename: Umberto
authors.initials: U
authors.lastname: De Marchi
authors.forename: Vanessa
authors.initials: V
authors.lastname: Checchetto
authors.forename: Manuela
authors.initials: M
authors.lastname: Zanetti
authors.forename: Enrico
authors.initials: E
authors.lastname: Teardo
authors.forename: Mario
authors.initials: M
authors.lastname: Soccio
authors.forename: Elide
authors.initials: E
authors.lastname: Formentin
authors.forename: Giorgio Mario
authors.initials: GM
authors.lastname: Giacometti
authors.forename: Donato
authors.initials: D
authors.lastname: Pastore
authors.forename: Mario
authors.initials: M
authors.lastname: Zoratti
authors.forename: Ildikò
authors.initials: I
authors.lastname: Szabò
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*; D014908:Triticum / Q000378:metabolism*
chemical_list: D015221:Potassium Channels; D000255:Adenosine Triphosphate