title: Detection of placental alpha microglobulin-1 versus insulin-like growth factor-binding protein-1 in amniotic fluid at term: a comparative study.
abstract: We compared two biochemical tests of premature rupture of membranes (PROM) in vitro: Actim PROM (Medix Biochemica, Kauniainen, Finland), which detects insulin-like growth factor binding protein-1, and AmniSure (AmniSure International LLC, Cambridge, MA), which detects placental alpha microglobulin-1. Samples of amniotic fluid were collected during caesarean section in 41 patients. A dilution series was prepared and both tests were performed twice at each dilution. Sensitivity, detection limit, response time, and reproducibility of both tests were compared. Both tests' sensitivity was 100% at dilution 1:10 and 1:20. AmniSure sensitivity was higher at dilution 1:40 and 1:80 ( P < 0.05). In 29 of 40 cases, AmniSure had a lower detection limit than Actim PROM. AmniSure response times were shorter and reproducibility was higher than Actim PROM ( P < 0.05). AmniSure had a lower detection limit of amniotic fluid than Actim PROM, with a shorter response time, a higher sensitivity, and a better reproducibility.
authors.affiliation: Service de gynécologie-obstétrique, Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, Bron, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Pollet-Villard
authors.forename: Régine
authors.initials: R
authors.lastname: Cartier
authors.forename: Pascal
authors.initials: P
authors.lastname: Gaucherand
authors.forename: Muriel
authors.initials: M
authors.lastname: Doret
mesh_terms: D000328:Adult; D000510:Alpha-Globulins / Q000032:analysis*; D000653:Amniotic Fluid / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis*; D005865:Gestational Age; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis*; D057230:Limit of Detection; D011237:Predictive Value of Tests; D011247:Pregnancy; D011446:Prospective Studies; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D000510:Alpha-Globulins; D015415:Biomarkers; D018970:Insulin-Like Growth Factor Binding Protein 1; C025734:chorionic alpha(1)-microglobulin