title: IgG and IgM antibodies to the refolded MOG(1-125) extracellular domain in humans.
abstract: Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOG(ED)(His)(6) showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.
authors.affiliation: Laboratorio Interdipartimentale di Chimica e Biologia di Peptidi e Proteine, Dipartimento di Scienze Farmaceutiche, Università degli Studi di Firenze, Via Ugo Schiff 6, 50019, Sesto Fiorentino, Firenze, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Gori
authors.forename: Barbara
authors.initials: B
authors.lastname: Mulinacci
authors.forename: Lara
authors.initials: L
authors.lastname: Massai
authors.forename: Carlo
authors.initials: C
authors.lastname: Avolio
authors.forename: Mariantonietta
authors.initials: M
authors.lastname: Caragnano
authors.forename: Elisa
authors.initials: E
authors.lastname: Peroni
authors.forename: Silvia
authors.initials: S
authors.lastname: Lori
authors.forename: Mario
authors.initials: M
authors.lastname: Chelli
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Papini
authors.forename: Paolo
authors.initials: P
authors.lastname: Rovero
authors.forename: Francesco
authors.initials: F
authors.lastname: Lolli
mesh_terms: D000328:Adult; D000818:Animals; D005110:Extracellular Space / Q000737:chemistry / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism*; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000737:chemistry / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000502:physiology; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D055815:Young Adult
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; C571326:MOG protein, human; C571328:Mog protein, rat; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D011994:Recombinant Proteins