title: Proteomic analysis of the GlnR-mediated response to nitrogen limitation in Streptomyces coelicolor M145.
abstract: GlnR is the global regulator of nitrogen assimilation in Streptomyces coelicolor M145 and other actinobacteria. Two-dimensional polyacrylamide gel electrophoresis analyses were performed to identify new GlnR target genes by proteomic comparison of wild-type S. coelicolor M145 and a ΔglnR mutant. Fifty proteins were found to be differentially regulated between S. coelicolor M145 and the ΔglnR mutant. These spots were identified by nanoHPLC-ESI-MS/MS and classified according to their cellular role. Most of the identified proteins are involved in amino acid biosynthesis and in carbon metabolism, demonstrating that the role of GlnR is not restricted to nitrogen metabolism. Thus, GlnR is supposed to play an important role in the global metabolic control of S. coelicolor M145.
authors.affiliation: Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Tiffert
authors.forename: Mirita
authors.initials: M
authors.lastname: Franz-Wachtel
authors.forename: Claudia
authors.initials: C
authors.lastname: Fladerer
authors.forename: Alfred
authors.initials: A
authors.lastname: Nordheim
authors.forename: Jens
authors.initials: J
authors.lastname: Reuther
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wohlleben
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Mast
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D009584:Nitrogen / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D018085:Regulon; D021241:Spectrometry, Mass, Electrospray Ionization; D048372:Streptomyces coelicolor / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C082451:GlnR protein, Streptomyces coelicolor; D020543:Proteome; D015534:Trans-Activators; D009584:Nitrogen