title: Vaccination of mice with chitosan nanogel-associated recombinant NcPDI against challenge infection with Neospora caninum tachyzoites.
abstract: The effects of nanogel encapsulation of recombinant NcPDI (recNcPDI) following vaccination of mice by intranasal or intraperitoneal routes and challenge infection with Neospora caninum tachyzoites were investigated. Nanogels were chitosan based, with an alginate or alginate-mannose surface. None of the mice receiving recNcPDI intraperitoneal (i.p.) (without nanogels) survived, whereas intranasal (i.n.) application protected 9 of 10 mice from disease. Association of recNcPDI with nanogels improved survival of i.p. vaccinated mice, but nanogels without recNcPDI gave similar protection levels. When nanogels were inoculated via the i.n. route, 80% of the mice were protected. Association of recNcPDI with the alginate-coated nanogels protected all mice against disease. Quantification of the cerebral parasite burden showed a significant reduction of parasite numbers in most experimental groups vaccinated i.n., except those vaccinated with alginate-mannose nanogels with or without recNcPDI. For i.p. vaccinated groups, no significant differences in cerebral infection densities were measured, but there was a reduction in the groups vaccinated with recNcPDI associated with both types of nanogels. Analysis of the immune responses of infected mice indicated that association of recNcPDI with nanogels altered the patterns of cytokine mRNA expression profiles, but had no major impact on the antibody subtype responses. Nevertheless, this did not necessarily relate to the protection.
authors.affiliation: Institute of Parasitology, Vetsuisse Faculty, University of Berne, Berne, Switzerland Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. karim.debache@ipa.unibe.ch
authors.forename: K
authors.initials: K
authors.lastname: Debache
authors.forename: C
authors.initials: C
authors.lastname: Kropf
authors.forename: C A
authors.initials: CA
authors.lastname: Schütz
authors.forename: L J
authors.initials: LJ
authors.lastname: Harwood
authors.forename: P
authors.initials: P
authors.lastname: Käuper
authors.forename: T
authors.initials: T
authors.lastname: Monney
authors.forename: N
authors.initials: N
authors.lastname: Rossi
authors.forename: C
authors.initials: C
authors.lastname: Laue
authors.forename: K C
authors.initials: KC
authors.lastname: McCullough
authors.forename: A
authors.initials: A
authors.lastname: Hemphill
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001921:Brain / Q000469:parasitology; D048271:Chitosan / Q000008:administration & dosage*; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage*; D005260:Female; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D000080385:Nanogels; D018688:Neospora / Q000201:enzymology / Q000276:immunology*; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D011094:Polyethyleneimine / Q000008:administration & dosage*; D019704:Protein Disulfide-Isomerases / Q000008:administration & dosage / Q000276:immunology*; D016052:Protozoan Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016019:Survival Analysis; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D004337:Drug Carriers; D000080385:Nanogels; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D048271:Chitosan; D019704:Protein Disulfide-Isomerases