title: Drugs of abuse detection in meconium: a comparison between ELISA and biochip microarray.
abstract: The results of meconium specimens and fortified samples screened for drugs of abuse by both enzyme-linked immunosorbent assay (ELISA, Immunalysis) and biochip microarray (Randox) methods were compared. The ELISA method was semi-automated using a TECAN Genesis. The Randox assay used the Randox Evidence Investigator system. Previously validated gas chromatography-mass spectrometry (GC-MS), GC-GC-MS, or liquid chromatography-MS-MS methods were used for confirmation and quantitation. Results from the two techniques compared well. Agreement of the Randox assay was greater than 90% when compared to the ELISA assay for all drug classes except cannabinoids (88%). Specificity of the biochip assay was slightly better for amphetamines and cocaine.
authors.affiliation: ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA. stephanie.marin@aruplab.com
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: Marin
authors.forename: Miles
authors.initials: M
authors.lastname: Merrell
authors.forename: Gwendolyn A
authors.initials: GA
authors.lastname: McMillin
mesh_terms: D002186:Cannabinoids / Q000032:analysis; D002853:Chromatography, Liquid; D003042:Cocaine / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D008694:Methamphetamine / Q000032:analysis; D040081:Protein Array Analysis / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D002186:Cannabinoids; D013287:Illicit Drugs; D008694:Methamphetamine; D003042:Cocaine