title: [The effect and mechanism of Salvia miltiorrhiza monomer IH764-3 on proliferation and collagen synthesis of hepatic stellate cells stimulated by H2O2].
abstract: OBJECTIVE
  To investigate the inhibiting role of Salvia miltiorrhiza monomer IH764-3 in proliferation and collagen synthesis and the effect on the expression of focal adhesion kinase in hepatic stellate cells (HSCs) stimulated by H2O2 in vitro, so as to provide evidences for preventing hepatic fibrosis.
  
  
  METHODS
  The effects of IH764-3 on the proliferation and collagen synthesis of HSCs were examined by 3H-TdR and 3H-proline incorporation assay, respectively. The expression of FAK mRNA was examined by RT-PCR in lysates of cultured HSCs.
  
  
  RESULTS
  The proliferation and collagen synthesis were inhibited in dose-dependent and time-dependent manners. The expressions of FAK mRNA in the IH764-3 (10 microg/ml, 20 microg/ml, 30 microg/ml, 40 microg/ml) groups were significantly lower than that in the control group.
  
  
  CONCLUSIONS
  The present results demonstrate that IH764-3 can inhibit HSC proliferation and collagen synthesis. Meanwhile the mRNA transcription of FAK is reduced significantly, FAK as a signal molecule, can be involved in the effect of IH764-3. This may be one of the anti-fibrotic mechanisms of Salvia Miltiorrhiza.
authors.affiliation: Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Jiang
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology; D027561:Salvia miltiorrhiza; D015398:Signal Transduction
chemical_list: D004365:Drugs, Chinese Herbal; C079053:IH 764-3; D003094:Collagen; D006861:Hydrogen Peroxide; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human