title: Na-doped β-tricalcium phosphate: physico-chemical and in vitro biological properties.
abstract: Synthetic calcium phosphate ceramics as β-tricalcium phosphate (Ca(3)(PO(4))(2); β-TCP) are currently successfully used in human bone surgery. The aim of this work was to evaluate the influence of the presence of sodium ion in β-TCP on its mechanical and biological properties. Five Na-doped-β-TCP [Ca(10.5-x/2)Na(x)(PO(4))(7), 0 ≤ x ≤ 1] microporous pellets were prepared via solid phase synthesis, and their physico-chemical data (lattice compacity, density, porosity, compressive strength, infrared spectra) denote an increase of the mechanical properties and a decrease of the solubility when the sodium content is raised. On the other hand, the in vitro study of MC3T3-E1 cell activity (morphology, MTS assay and ALP activity) shows that the incorporation of sodium does not modify the bioactivity of the β-TCP. These results strongly suggest that Na-doped-β-TCP appear to be good candidates for their use as bone substitutes.
authors.affiliation: INSERM, UMR 791, LIOAD, Faculté de Chirurgie Dentaire, Université de Nantes, BP 84215, 44042, Nantes Cedex 1, France.
authors.forename: Laëtitia
authors.initials: L
authors.lastname: Obadia
authors.forename: Marion
authors.initials: M
authors.lastname: Julien
authors.forename: Sophie
authors.initials: S
authors.lastname: Quillard
authors.forename: Thierry
authors.initials: T
authors.lastname: Rouillon
authors.forename: Paul
authors.initials: P
authors.lastname: Pilet
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.forename: Bruno
authors.initials: B
authors.lastname: Bujoli
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Bouler
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry; D001842:Bone and Bones / Q000473:pathology; D002118:Calcium / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D002627:Chemistry, Physical / Q000379:methods; D006801:Humans; D007477:Ions; D008422:Materials Testing; D051379:Mice; D016062:Porosity; D012964:Sodium / Q000737:chemistry*; D013314:Stress, Mechanical
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D007477:Ions; C485817:beta-tricalcium phosphate; D012964:Sodium; D000469:Alkaline Phosphatase; D002118:Calcium