title: Steps towards a mechanistic understanding of respiratory temperature responses.
abstract: Temperature crucially affects the speed of metabolic processes in poikilotherm organisms, including plants. The instantaneous temperature responses of O(2)-reduction and CO(2)-release can be approximated by Arrhenius kinetics, even though respiratory gas exchange of plants is the net effect of many constituent biochemical processes. Nonetheless, the classical Arrhenius equation must be modified to account for a dynamic response to measurement temperatures. We show that this dynamic response is readily explained by combining Arrhenius and Michaelis-Menten kinetics, as part of a fresh appraisal of metabolic interpretations of instantaneous temperature responses. In combination with recent experimental findings, we argue that control of mitochondrial electron flow is shared among cytochrome oxidase and alternative oxidase under in vivo conditions, and is continuously coordinated. In this way, upstream carbohydrate metabolism and downstream electron transport appear to be optimized according to the demand of ATP, TCA-cycle intermediates and anabolic reducing power under differing metabolic states. We provide a link to the 'Growth and Maintenance Paradigm' of respiration and argue that respiratory temperature responses can be used as a tool to probe metabolic states of plant tissue, such that we can learn more about the mechanisms that govern longer-term acclimatization responses of plant metabolism.
authors.affiliation: Institute of Forest Botany, Chair of Tree Physiology, Albert-Ludwigs-University Freiburg, Georges-Koehler-Allee 53-54, D-79110 Freiburg, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Kruse
authors.affiliation: Institute of Forest Botany, Chair of Tree Physiology, Albert-Ludwigs-University Freiburg, Georges-Koehler-Allee 53-54, D-79110 Freiburg, Germany.
authors.forename: Heinz
authors.initials: H
authors.lastname: Rennenberg
authors.affiliation: Faculty of Agriculture, Food and Natural Resources, The University of Sydney, Sydney, NSW 2006, Australia.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Adams
mesh_terms: D000064:Acclimatization / Q000502:physiology*; D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D019069:Cell Respiration / Q000502:physiology; D004734:Energy Metabolism; D008928:Mitochondria / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*; D013696:Temperature
chemical_list: D002245:Carbon Dioxide; D010088:Oxidoreductases; D010100:Oxygen