title: Evaluation of the compact dry X-SA method for enumerating Staphylococcus aureus in artificially contaminated food samples.
abstract: Compact Dry X-SA (CD-XSA), a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Staphylococcus aureus, was evaluated. A total of 50 S. aureus strains, which were studied for the inclusivity study, grew as blue-colored colonies on the CD-XSA. When 114 bacteria other than S. aureus and 3 yeasts were inoculated for the exclusivity study, 37 strains produced white colonies, and 4 strains produced blue colonies, and 3 strains produced magenta colonies, while 73 other strains failed to grow. The CD-XSA method was compared with the mannitol salt agar with egg yolk (MSEY) method, the Baird-Parker agar (BP) method and the 3M Petrifilm(TM) STX (3M-STX) method in 105 artificially contaminated food samples. The correlation coefficients between CD-XSA and MSEY, CD-XSA and BP, and CD-XSA and 3M-STX were 0.945, 0.960 and 0.977, respectively.
authors.affiliation: Nissui Pharmaceutical Co., Ltd, Ibaraki, Japan. h-teramura@yki.nissui-pharm.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Teramura
authors.forename: Shingo
authors.initials: S
authors.lastname: Mizuochi
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kodaka
mesh_terms: D058491:Bacterial Load / Q000379:methods*; D003470:Culture Media; D005516:Food Microbiology; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D003470:Culture Media