title: Fluorescence energy transfer efficiency in labeled yeast cytochrome c: a rapid screen for ion biocompatibility in aqueous ionic liquids.
abstract: A fluorescence energy transfer "de-quenching" assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.
authors.affiliation: Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37931, USA.
authors.forename: Sheila N
authors.initials: SN
authors.lastname: Baker
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.forename: Siddharth
authors.initials: S
authors.lastname: Pandey
authors.forename: William T
authors.initials: WT
authors.lastname: Heller
authors.forename: Frank V
authors.initials: FV
authors.lastname: Bright
authors.forename: Gary A
authors.initials: GA
authors.lastname: Baker
mesh_terms: D045304:Cytochromes c / Q000737:chemistry*; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D052578:Ionic Liquids; D012235:Rhodamines; C005358:tetramethylrhodamine; D045304:Cytochromes c