title: [Development of specific antibodies against ciprofloxacin].
abstract: OBJECTIVE
  To prepare specific antibodies against ciprofloxacin (CIP) and characterize the antibodies properities with ELISA, then lay the foundation for development of CIP rapid detection kit.
  
  
  METHODS
  New Zealand white rabbits and BALB/c mice were immunized with CIP-cBSA conjugate, CIP-nOVA and CIP-cOVA serving as coating antigen were used to screen antisera, indirect (competitive) ELISA was developed to determine the titer and specificity of the antisera.
  
  
  RESULTS
  Seven antisera against CIP were obtained, two of them were high specific to CIP with IC(50); 1 μg/L, the titer of which was 4 000 and 2 000, respectively.The antisera had some degree cross-reactivity with ENR, NOR and OFL, but had hardly cross-reactivity with PEN, KAN and QEN.
  
  
  CONCLUSIONS
  The antibodies with high specificity developed in this study can be used to develop CIP detecting kit.
authors.affiliation: Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agriculture Sciences, Beijing 100081, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Sheng-liang
authors.initials: SL
authors.lastname: Deng
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Liu
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Qiu
authors.forename: Shu-ming
authors.initials: SM
authors.lastname: Yang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D002939:Ciprofloxacin / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
chemical_list: D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D002939:Ciprofloxacin