title: Isolation of cycloamylose by iodine affinity capillary electrophoresis.
abstract: In contrast to α-, β- and γ-cyclodextrins, little information is available on the isolation and separation of cycloamylose (CA) with degree of polymerization (DP) larger than 22. The objective of the current study was to develop a new iodine affinity capillary electrophoresis (CE) for separation of CA with DP of 22-42, which was based on the formation of CA-iodine inclusion complexes, CA with twisted conformations made complicated mobility behaviors on CE instead of merely size dependent. The influences of iodide/iodine ratio, iodine concentration, pH, ion strength of running phosphate buffer, voltage, and temperature on the peak resolution and electrophoretic mobility were further investigated. Our results suggest that iodine affinity capillary electrophoresis provides a versatile and selective tool for the isolation and analysis of CA with DP from 22 to 42.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: JinPeng
authors.initials: J
authors.lastname: Wang
authors.forename: AiQuan
authors.initials: A
authors.lastname: Jiao
authors.forename: YaoQi
authors.initials: Y
authors.lastname: Tian
authors.forename: XueMing
authors.initials: X
authors.lastname: Xu
authors.forename: ZhengYu
authors.initials: Z
authors.lastname: Jin
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007455:Iodine / Q000737:chemistry*; D016015:Logistic Models; D008970:Molecular Weight; D058105:Polymerization
chemical_list: D003505:Cyclodextrins; D007455:Iodine