title: Anti-inflammatory actions of a taurine analogue, ethane β-sultam, in phagocytic cells, in vivo and in vitro.
abstract: The ability of a taurine prodrug, ethane β-sultam, to reduce cellular inflammation has been investigated, in vitro, in primary cultures of alveolar macrophages and an immortilised N9 microglial cell line and in vivo in an animal model of inflammation and control rats. Ethane β-sultam showed enhanced ability to reduce the inflammatory response in alveolar macrophages, as assayed by the lipopolysaccharide-stimulated-nitric oxide release, (LPS stimulated-NO), in comparison to taurine both in vitro (10 nM, 50 nM) and in vivo (0.15 mmol/kg/day by gavage). In addition, ethane β-sultam, (50, 100 and 1000 nM) significantly reduced LPS-stimulated glutamate release from N9 microglial cells to a greater extent than taurine. The anti-inflammatory response of taurine was shown to be mediated via stabilisation of IkBα. The use of a taurine prodrug as therapeutic agents, for the treatment of neurological conditions, such as Parkinson's and Alzheimer's disease and alcoholic brain damage, where activated phagocytic cells contribute to the pathogenesis, may be of great potential.
authors.affiliation: Biologie du Comportement, Université Catholique de Louvain, Belgium. roberta.ward@uclouvain.be
authors.forename: Roberta J
authors.initials: RJ
authors.lastname: Ward
authors.forename: Frederic
authors.initials: F
authors.lastname: Lallemand
authors.forename: Philippe
authors.initials: P
authors.lastname: de Witte
authors.forename: Robert R
authors.initials: RR
authors.lastname: Crichton
authors.forename: Jacques
authors.initials: J
authors.lastname: Piette
authors.forename: Keith
authors.initials: K
authors.lastname: Tipton
authors.forename: Karl
authors.initials: K
authors.lastname: Hemmings
authors.forename: Arnaud
authors.initials: A
authors.lastname: Pitard
authors.forename: Mike
authors.initials: M
authors.lastname: Page
authors.forename: Laura
authors.initials: L
authors.lastname: Della Corte
authors.forename: Deanna
authors.initials: D
authors.lastname: Taylor
authors.forename: David
authors.initials: D
authors.lastname: Dexter
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D004980:Ethane / Q000031:analogs & derivatives / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000737:chemistry / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D011355:Prodrugs / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013449:Sulfonamides / Q000737:chemistry / Q000494:pharmacology*; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018836:Inflammation Mediators; D011355:Prodrugs; D013449:Sulfonamides; C480101:beta-sultam; D013654:Taurine; D004980:Ethane