title: Crystallization and preliminary X-ray crystallographic analysis of human quinolinate phosphoribosyltransferase.
abstract: Quinolinate phosphoribosyltransferase (QPRTase) is a key NAD-biosynthetic enzyme which catalyzes the transfer of quinolinic acid to 5-phosphoribosyl-1-pyrophosphate, yielding nicotinic acid mononucleotide. Homo sapiens QPRTase (Hs-QPRTase) appeared as a hexamer during purification and the protein was crystallized. Diffraction data were collected and processed at 2.8 Å resolution. Native Hs-QPRTase crystals belonged to space group P2(1), with unit-cell parameters a=76.2, b=137.1, c=92.7 Å, β=103.8°. Assuming the presence of six molecules in the asymmetric unit, the calculated Matthews coefficient is 2.46 Å3 Da(-1), which corresponds to a solvent content of 49.9%.
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Gil Bu
authors.initials: GB
authors.lastname: Kang
authors.forename: Mun-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Hyung-Seop
authors.initials: HS
authors.lastname: Youn
authors.forename: Jun Yop
authors.initials: JY
authors.lastname: An
authors.forename: Jung-Gyu
authors.initials: JG
authors.lastname: Lee
authors.forename: Kyoung Ryoung
authors.initials: KR
authors.lastname: Park
authors.forename: Sung Hang
authors.initials: SH
authors.lastname: Lee
authors.forename: Yongseong
authors.initials: Y
authors.lastname: Kim
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Fukuoka
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Eom
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D009243:NAD / Q000096:biosynthesis; D010430:Pentosyltransferases / Q000737:chemistry* / Q000378:metabolism; D020836:Protein Structure, Quaternary; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D009243:NAD; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)