title: The promotion of neurite formation in Neuro2A cells by mouse Mob2 protein.
abstract: The molecular mechanism of neuritogenesis has been extensively studied but remains unclear. In this study, we identified Mob2 protein which plays a significant role in promoting neurite formation in Neuro2A (N2A) cells. Our results showed that Mob2 was expressed in developing N2A cells. To study whether Mob2 was involved in neurite formation, we downregulated Mob2 expression using RNA interference and found that neurite formation decreased in low serum induced N2A cells. In addition, we found that overexpression of Mob2 promoted neurite formation in N2A cells. Furthermore, downregulation of Mob2 expression altered the rearrangement of the actin cytoskeleton and decreased the expression of phosphorylated Moesin. Together, these results provide information on the role of Mob2 in mediating neurite formation.
authors.affiliation: Department of Life Science, Tunghai University, Taichung, Taiwan, Republic of China.
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Lin
authors.forename: Mingli
authors.initials: M
authors.lastname: Hsieh
authors.forename: Seng-Sheen
authors.initials: SS
authors.lastname: Fan
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D015536:Down-Regulation; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection; D015854:Up-Regulation
chemical_list: D008840:Microfilament Proteins; C556585:Mob2 protein, mouse; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C071058:moesin