title: Analysis of cytoplasmic membrane proteome of Streptococcus pneumoniae by shotgun proteomic approach.
abstract: In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferase systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae.
authors.affiliation: Devision of Life Science, Korea Basic Science Institute, Daejeon, 305-806, Republic of Korea.
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Yun
authors.forename: Sang-Oh
authors.initials: SO
authors.lastname: Kwon
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Leem
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Chi-Young
authors.initials: CY
authors.lastname: Yun
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry*; D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D013296:Streptococcus pneumoniae / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014461:Ultracentrifugation
chemical_list: D001426:Bacterial Proteins; D020543:Proteome