title: Influence of intermittent administration of parathyroid hormone on muscle tissue and bone healing in orchiectomized rats or controls.
abstract: Influence of human parathyroid hormone (hPTH 1-34) on muscle and bone healing was studied in either orchiectomized (Orx at 8 months of age) or sham-operated male rats. Eleven-month-old Sprague-Dawley rats underwent bilateral transverse metaphyseal osteotomy of tibia and were divided into four groups (n=12): 1) sham-vehicle, 2) sham group-PTH everyday, 3) Orx-vehicle, 4) Orx-PTH everyday, and 5) Orx-PTH every other day. PTH dosage was 40  μg/kg body weight. After 5 weeks, fiber cross-sectional area, capillary density, and enzyme activity (lactate dehydrogenase, citrate synthase, and complex I) were measured in soleus (MS), gastrocnemius (MG), and longissimus (ML) muscles; tibiae were analyzed by computed tomographical, histological, and gene expression analyses. The effect of PTH in all rats was increased serum osteocalcin, cortical and callus densities and callus area. In sham rats capillary density was increased in limb muscles (MS: 1.3-1.7, MG: 1.2-1.4 capillaries/fiber), and rate of osseous bridging of osteotomy was enhanced (67-100%). In Orx rats serum creatine kinase was decreased (6670-2847 U/l), and bone genes (Igf-1, osteoprotegerin, and receptor activator of nuclear factor kB ligand) were up-regulated. Cross-sectional area, enzyme activity, food intake, weight of body, visceral organs, adipose tissue, MG, and MS were not affected by PTH. PTH had a favorable effect on muscle capillary density and improved bone healing being more effective in sham rats and having no adverse systemic effect. The effect was less if PTH was applied every other day. The findings may show up trends for therapeutic treatment of male patients.
authors.affiliation: Department of Trauma Surgery and Reconstructive Surgery, University of Goettingen, Robert-Koch 40, 37075 Goettingen, Germany. komrakova@yahoo.com
authors.forename: Marina
authors.initials: M
authors.lastname: Komrakova
authors.forename: Carsten
authors.initials: C
authors.lastname: Krischek
authors.forename: Michael
authors.initials: M
authors.lastname: Wicke
authors.forename: Stephan
authors.initials: S
authors.lastname: Sehmisch
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tezval
authors.forename: Maximillian
authors.initials: M
authors.lastname: Rohrberg
authors.forename: Thomas
authors.initials: T
authors.lastname: Brandsch
authors.forename: Klaus M
authors.initials: KM
authors.lastname: Stuermer
authors.forename: Ewa K
authors.initials: EK
authors.lastname: Stuermer
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D002196:Capillaries / Q000187:drug effects; D002853:Chromatography, Liquid; D004435:Eating / Q000187:drug effects; D017102:Fracture Healing / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects*; D009919:Orchiectomy; D010012:Osteogenesis / Q000187:drug effects*; D010027:Osteotomy; D010281:Parathyroid Hormone / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D013739:Testosterone / Q000097:blood; D013977:Tibia / Q000187:drug effects*; D013978:Tibial Fractures
chemical_list: D010281:Parathyroid Hormone; D013739:Testosterone