title: Analysis of β-agonists by HPLC/ESI-MS(n) in horse doping control.
abstract: A sensitive method using LC/ESI-MS(n) has been developed on a quadrupole linear ion trap mass analyser for the detection of nine β(2) agonists (cimaterol, clenbuterol, fenoterol, formoterol, mabuterol, terbutaline, ractopamine, salbutamol and salmeterol) in horse urine. The method consists of solid-phase extraction on CSDAU cartridges before analysis by LC/ESI-MS(n) . The efficiency of extraction combined with the sensitivity and the selectivity of MS(n) allowed the detection of these compounds at pg/mL levels. Administration studies of fenoterol and formoterol are reported and show their possible detection after inhalation. The method is applicable for screening and confirmatory analysis.
authors.affiliation: LCH, Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370 Verrières le Buisson, France.
authors.forename: P
authors.initials: P
authors.lastname: Garcia
authors.forename: A-C
authors.initials: AC
authors.lastname: Paris
authors.forename: J
authors.initials: J
authors.lastname: Gil
authors.forename: M-A
authors.initials: MA
authors.lastname: Popot
authors.forename: Y
authors.initials: Y
authors.lastname: Bonnaire
mesh_terms: D000318:Adrenergic beta-Agonists / Q000737:chemistry / Q000652:urine*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004300:Doping in Sports / Q000517:prevention & control*; D004983:Ethanolamines / Q000737:chemistry / Q000652:urine*; D006736:Horses / Q000652:urine*; D058573:Performance-Enhancing Substances / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D058573:Performance-Enhancing Substances