title: Identification of HACEK clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and accurate tool for the identification of many microorganisms. We assessed this technology for the identification of 103 Haemophilus parainfluenzae, Aggregatibacter aphrophilus, Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae (HACEK) clinical isolates and 20 Haemophilus influenzae clinical isolates. Ninety-three percent of HACEK organisms were identified correctly to the genus level using the Bruker database, and 100% were identified to the genus level using a custom database that included clinical isolates.
authors.affiliation: University of Utah School of Medicine, Department of Pathology, Salt Lake City, UT 84108, USA.
authors.forename: Marc Roger
authors.initials: MR
authors.lastname: Couturier
authors.forename: Elvina
authors.initials: E
authors.lastname: Mehinovic
authors.forename: Ann C
authors.initials: AC
authors.lastname: Croft
authors.forename: Mark A
authors.initials: MA
authors.lastname: Fisher
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D044066:Cardiobacterium / Q000737:chemistry* / Q000145:classification; D016124:Eikenella corrodens / Q000737:chemistry* / Q000145:classification; D006190:Haemophilus / Q000737:chemistry* / Q000145:classification; D006801:Humans; D017882:Kingella kingae / Q000737:chemistry* / Q000145:classification; D015661:Pasteurellaceae / Q000737:chemistry* / Q000145:classification; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*