title: [Purification process of proanthrocyanidin from rapeseed with macroporous resins].
abstract: The characteristics of absoption and desorption of four classes (five kinds) of macroporous resins in the solution of rapeseed proanthrocyanidin were compared. The results indicated that the weak polar macroporous resins AB-8 and HPD450 were better than the other three macroporous resins in some characteristics of absorption and desorption. In this experiment, the purity of rapeseed proanthrocyanidin prepared only by AB-8 macroporous resin column was higher than only by HPD450 column. However,the yield was lower. Using two steps of purification (first HPD450 column and second AB-8 column) obtained better results. The yield and purity were 62. 5% and 84% ,respectively. After using mixed resin column (50% HPD450 and 50% AB-8), the yield and purity were 75.2% and 80.3%, respectively. The last results might be used in industry.
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai 200092, China. joyee1022@163.com
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Wu
authors.forename: Chun-tang
authors.initials: CT
authors.lastname: Xia
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Ding
mesh_terms: D000327:Adsorption; D001547:Benzaldehydes / Q000737:chemistry; D029741:Brassica rapa / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D016062:Porosity; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry* / Q000145:classification; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D001547:Benzaldehydes; D046911:Macromolecular Substances; D044945:Proanthocyanidins; D012117:Resins, Synthetic; D000431:Ethanol