title: Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania.
abstract: Outbreaks of African swine fever (ASF) have been reported in the past from several countries in sub-Saharan Africa. The aim of this study was to genotype ASF viruses (ASFVs) from the 2008 outbreak in Morogoro and Dar es Salaam regions of Tanzania. Tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with PCR and genome sequencing methods using ASFV-specific primer sets. Nucleotide sequence data were obtained for the B646L (p72), E183L (p54) and the variable region of the B602L gene sequences. Phylogenetic analyses based on DNA sequences showed that the 2008 Tanzanian isolates belonged to p72 genotype XV and clustered together with those derived from the 2001 outbreak in Tanzania. Analysis of the tetrameric amino acid repeat regions within the variable region of the B602L gene showed that the repeat signature of the 2008 Tanzanian ASFV was unique and contained three novel tetramers (U = NIDT/NTDT and X = NTDI). Epidemiological investigation suggested that transportation of live pigs continues to play an active role in the epidemiology of ASF in Tanzania. It is recommended that future control of ASF spread in Tanzania should focus on the early detection and confirmation of the disease, prompt institution of quarantine measures, culling and proper disposal of infected and in-contact animals and decontamination of affected premises.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, Morogoro, Tanzania. gmisinzo@suanet.ac.tz
authors.forename: G
authors.initials: G
authors.lastname: Misinzo
authors.forename: J
authors.initials: J
authors.lastname: Magambo
authors.forename: J
authors.initials: J
authors.lastname: Masambu
authors.forename: M G
authors.initials: MG
authors.lastname: Yongolo
authors.forename: J
authors.initials: J
authors.lastname: Van Doorsselaere
authors.forename: H J
authors.initials: HJ
authors.lastname: Nauwynck
mesh_terms: D000357:African Swine Fever / Q000453:epidemiology* / Q000821:virology; D000358:African Swine Fever Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013636:Tanzania / Q000453:epidemiology