title: RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.).
abstract: Sex pheromone production is regulated by pheromone biosynthesis-activating neuropeptide (PBAN) in many lepidopteran species. We cloned a PBAN receptor (Plx-PBANr) gene from the female pheromone gland of the diamondback moth, Plutella xylostella (L.). Plx-PBANr encodes 338 amino acids and has conserved structural motifs implicating in promoting G protein coupling and tyrosine-based sorting signaling along with seven transmembrane domains, indicating a typical G protein-coupled receptor. The expression of Plx-PBANr was found only in the pheromone gland of female adults among examined tissues and developmental stages. Heterologous expression in human uterus cervical cancer cells revealed that Plx-PBANr induced significant calcium elevation when challenged with Plx-PBAN. Female P. xylostella injected with double-stranded RNA specific to Plx-PBANr showed suppression of the receptor gene expression and exhibited significant reduction in pheromone biosynthesis, which resulted in loss of male attractiveness. Taken together, the identified PBAN receptor is functional in PBAN signaling via calcium secondary messenger, which leads to activation of pheromone biosynthesis and male attraction.
authors.affiliation: Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Korea.
authors.forename: Dae-Weon
authors.initials: DW
authors.lastname: Lee
authors.forename: Sony
authors.initials: S
authors.lastname: Shrestha
authors.forename: A Young
authors.initials: AY
authors.lastname: Kim
authors.forename: Seok Joo
authors.initials: SJ
authors.lastname: Park
authors.forename: Chang Yeol
authors.initials: CY
authors.lastname: Yang
authors.forename: Yonggyun
authors.initials: Y
authors.lastname: Kim
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Koh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D015536:Down-Regulation; D005260:Female; D006801:Humans; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism; D010802:Phylogeny; D034622:RNA Interference; D018013:Receptors, Neuropeptide / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D012724:Sex Attractants / Q000096:biosynthesis*; D012726:Sexual Behavior, Animal
chemical_list: D019476:Insect Proteins; D009479:Neuropeptides; D018013:Receptors, Neuropeptide; D012724:Sex Attractants