title: [Establishment of the model of oxygen-glucose deprivation in vitro rat hippocampal neurons].
abstract: OBJECTIVE
  To establish the model of oxygen-glucose deprivation in vitro rat hippocampal neurons.
  
  
  METHODS
  The hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5-4 h and then cultured with original medium in normoxia for 24 h. Auto-biochemical analyzer determined LDH activity. The change of neuronal morphology and neuron survival were observed by converted contrast microscope and assessed by photography analysis system. Neuron apoptosis was detected by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method.
  
  
  RESULTS
  The neurons swelled, LDH release increased and neuron survival decreased after gradually oxygen-glucose deprivation. The percentage of apoptosis increased obviously 24 h after recovering the supply of oxygen and glucose.
  
  
  CONCLUSIONS
  The model of oxygen-glucose deprivation in vitro rat hippocampal neurons is established successfully by using the modified ACSF (artificial cerebral spinal fluid) with serum and glucose free.
authors.affiliation: Department of Neurobiology, Institute of Basic Medical Sciences, Academny of Military Medical Sciences, Bejing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Ai-shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Zi-min
authors.initials: ZM
authors.lastname: Ma
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D010100:Oxygen / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005947:Glucose; D010100:Oxygen