title: Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8.
abstract: A bacterial isolate, designated as DQ8, was found capable of degrading diesel, crude oil, n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in petroleum. Strain DQ8 was assigned to the genus Pseudomonas aeruginosa based on biochemical and genetic data. The metabolites identified from n-docosane as substrate suggested that P. aeruginosa DQ8 could oxidize n-alkanes via a terminal oxidation pathway. P. aeruginosa DQ8 could also degrade PAHs of three or four aromatic rings. The metabolites identified from fluorene as substrate suggested that P. aeruginosa DQ8 may degrade fluorene via two pathways. One is monooxygenation at C-9 of fluorene, and the other is initiated by dioxygenation at C-3 and C-4 of fluorene. P. aeruginosa DQ8 should be of great practical significance both in bioremediation of oil-contaminated soils and biotreatment of oil wastewater.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Hou
authors.forename: Chunyu
authors.initials: C
authors.lastname: Yang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Fei
authors.initials: F
authors.lastname: Tao
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000473:Alkanes / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005449:Fluorenes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D010084:Oxidation-Reduction; D010578:Petroleum / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D000473:Alkanes; D005449:Fluorenes; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; C041509:fluorene