title: Recent transposition activity of Xenopus T2 family miniature inverted-repeat transposable elements.
abstract: To investigate the recent transposition activity of T2 family miniature inverted-repeat transposable elements (MITEs) in Xenopus tropicalis (Western clawed frog), we analyzed the intraspecific polymorphisms associated with MITE insertion in X. tropicalis for three subfamilies of the T2 family (T2-A1, T2-C, and T2-E). A high frequency of MITE-insertion polymorphisms was observed at the T2-A1 (50%) and T2-C insertion loci (60%), but none were noted at the T2-E insertion locus (0%). Analyses of the collected data indicated that members of the T2-A1 and T2-C subfamilies may be currently active in the host species. Identification of these active transpositions will help us in understanding the mechanisms underlying the long-term survival (over several tens of millions of years) of the T2-A1 and T2-C subfamilies.
authors.affiliation: Graduate School of Integrated Arts and Sciences, Hiroshima University, Kagamiyama 1-7-1, Higashi-Hiroshima, Hiroshima 739-8521, Japan. akirahs@hiroshima-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Hikosaka
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nishimura
authors.forename: Tomoe
authors.initials: T
authors.lastname: Hikosaka-Katayama
authors.forename: Akira
authors.initials: A
authors.lastname: Kawahara
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D019143:Evolution, Molecular; D055029:Inverted Repeat Sequences / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014981:Xenopus / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements