title: Immunological regulation of Chinese herb Guizhi Fuling Capsule on rat endometriosis model.
abstract: OBJECTIVE
  To investigate the immunological regulation of Guizhi Fuling Capsule (GZFLC) on rat endometriosis.
  
  
  METHODS
  Twenty-seven rats, in which endometriotic implants were induced by transplanting autologous uterine tissue to the peritoneum, were randomly divided into three groups equally: (1) the GZFLC group of low dose (480 mg/kg/day); (2) the GZFLC group of high dose (1,920 mg/kg/day); and (3) the model group(saline solution). Another 10 rats were treated as sham operation group. After rats were treated for four weeks, we examined the alterations of implants volume, the percentage of CD4(+) T lympholeukocyte, the activity of NK cell and the expression of cytokines (MCP-1 and ICAM-1) on each group.
  
  
  RESULTS
  Statistical analysis showed that posttreatment volumes were significantly reduced compared with pretreatment in GZFLC groups, whereas there was no significant change in the model group. The percentage of CD4(+) T lympholeukocyte and the activity of NK cell in GZFLC groups significantly increased to the level of the sham group compared with the model. RT-PCR and immunohistochemistry showed that the endometria of the sham operation and treatment groups were similar on expression level of MCP-1 and ICAM-1.
  
  
  CONCLUSIONS
  GZFLC plays an important role in the regression of endometriotic implants by immunological regulation in the rat model.
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210097, Jiangsu, China.
authors.forename: Xiayun
authors.initials: X
authors.lastname: Ji
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Xueting
authors.initials: X
authors.lastname: Cai
authors.forename: Wuguang
authors.initials: W
authors.lastname: Lu
authors.forename: Chunping
authors.initials: C
authors.lastname: Hu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiawei
authors.initials: X
authors.lastname: Cheng
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Gu
authors.forename: Guiping
authors.initials: G
authors.lastname: Wan
authors.forename: Shuangquan
authors.initials: S
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
mesh_terms: D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018932:Chemokine CCL2 / Q000378:metabolism; D017931:DNA Primers; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004715:Endometriosis / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018932:Chemokine CCL2; D017931:DNA Primers; D004365:Drugs, Chinese Herbal; C525079:guizhi-fuling; D018799:Intercellular Adhesion Molecule-1