title: Different inflammatory phenotypes in adults and children with acute asthma.
abstract: Inflammatory phenotypes are recognised in stable adult asthma, but are less well established in childhood and acute asthma. Additionally, Chlamydophila pneumoniae infection as a cause of noneosinophilic asthma is controversial. This study examined the prevalence of inflammatory phenotypes and the presence of current C. pneumoniae infection in adults and children with stable and acute asthma. Adults with stable (n=29) or acute (n=22) asthma, healthy adults (n=11), children with stable (n=49) or acute (n=28) asthma, and healthy children (n=9) underwent clinical assessment and sputum induction. Sputum was assessed for inflammatory cells, and DNA was extracted from sputum cell suspensions and supernatants for C. pneumoniae detection using real-time PCR. The asthma phenotype was predominantly eosinophilic in children with acute asthma (50%) but neutrophilic in adults with acute asthma (82%). Paucigranulocytic asthma was the most common phenotype in both adults and children with stable asthma. C. pneumoniae was not detected in 99% of samples. The pattern of inflammatory phenotypes differs between adults and children, with eosinophilic inflammation being more prevalent in both acute and stable childhood asthma, and neutrophilic inflammation being the dominant pattern of acute asthma in adults. The aetiology of neutrophilic asthma is unknown and is not explained by the presence of current active C. pneumoniae infection.
authors.affiliation: Dept of Respiratory Medicine, John Hunter Hospital, Newcastle, NSW 2310, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: X Y
authors.initials: XY
authors.lastname: He
authors.forename: K J
authors.initials: KJ
authors.lastname: Baines
authors.forename: L P
authors.initials: LP
authors.lastname: Gunawardhana
authors.forename: J L
authors.initials: JL
authors.lastname: Simpson
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: P G
authors.initials: PG
authors.lastname: Gibson
mesh_terms: D000208:Acute Disease; D000328:Adult; D000402:Airway Obstruction / Q000473:pathology; D001249:Asthma / Q000276:immunology / Q000473:pathology*; D002648:Child; D023521:Chlamydophila Infections / Q000378:metabolism; D016993:Chlamydophila pneumoniae / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D010641:Phenotype; D015272:Pulmonary Medicine / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013183:Sputum
chemical_list: D004247:DNA