title: Proliferation and chondrogenic differentiation of CD105-positive enriched rat synovium-derived mesenchymal stem cells in three-dimensional porous scaffolds.
abstract: Stem cell-based tissue engineering has provided an alternative strategy to treat cartilage lesions, and synovium-derived mesenchymal stem cells (SMSCs) are considered as a promising cell source for cartilage repair. In this study, the SMSCs were isolated from rat synovium, and CD105-positive (CD105(+)) cells were enriched using magnetic activated cell sorting. Sorted cells were subsequently seeded onto the chitosan-alginate composite three-dimensional (3D) porous scaffolds and cultured in chondrogenic culture medium in the presence of TGF-β₃ and BMP-2 for 2 weeks in vitro. After 2 weeks in culture, scanning electron microscopy results showed that cells attached and proliferated well on scaffolds, and secreted extracellular matrix were also observed. From day 7 to day 14, the total DNA and glucosaminoglycan content of the cells cultured in scaffolds were found to have increased significantly, and cell cycle analyses revealed that the percentage of cells in the S and G2/M phases increased and the percentage of cells in the G0/G1 phase decreased. Compared with non-sorted cells, the sorted cells cultured in scaffolds underwent more chondrogenic differentiation, as evidenced by higher expression of type II collagen and Sox9 at the protein and mRNA levels. The results suggest that CD105(+) enriched SMSCs may be a potential cell source for cartilage tissue engineering, and the chitosan-alginate composite 3D porous scaffold could provide a favorable microenvironment for supporting proliferation and chondrogenic differentiation of cells.
authors.affiliation: Department of Orthopedics, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.forename: Anmin
authors.initials: A
authors.lastname: Chen
authors.forename: Hongbo
authors.initials: H
authors.lastname: You
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Fengjing
authors.initials: F
authors.lastname: Guo
mesh_terms: D000464:Alginates; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D049109:Cell Proliferation; D048271:Chitosan; D020219:Chondrogenesis / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D000071063:Endoglin; D020723:Glucuronic Acid; D006025:Glycosaminoglycans / Q000378:metabolism; D006603:Hexuronic Acids; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000464:Alginates; D024043:Collagen Type II; D017931:DNA Primers; D000071063:Endoglin; C501740:Eng protein, rat; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D047908:Intracellular Signaling Peptides and Proteins; D020723:Glucuronic Acid; D004247:DNA; D048271:Chitosan