title: Initial acidic pH is critical for mycelial cultures and functional exopolysaccharide production of an edible mushroom, Laetiporus sulphureus var. miniatus JM 27.
abstract: We conducted a time course experiment on mycelial cultures of Laetiporus sulphureus var. miniatus. The strain showed significant survival in an initial pH range of 2.0 to 7.0 for 24 days, during which time oxalic acid was accumulated. A structural analysis of purified exopolysaccharide suggested that it contained 96.1% glucose, and the mode of linkage was mainly → 4-Glcp-(1 → units, with branches at the C-6 position consisting of a Glcp-(1 → 4) linked side chain. An exopolysaccharide purified from the acidophilic strain was added to cultured U937 cells, resulting in significantly increased transcription levels of p53 and p21 genes.
authors.affiliation: Department of Medical Bioscience, Dong-A University, Busan, 604-714, Republic of Korea.
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Seo
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hye Hyeon
authors.initials: HH
authors.lastname: Lee
authors.forename: Sung Ryeal
authors.initials: SR
authors.lastname: Kim
authors.forename: Byoung Won
authors.initials: BW
authors.lastname: Kang
authors.forename: Jeong Uck
authors.initials: JU
authors.lastname: Park
authors.forename: En Ju
authors.initials: EJ
authors.lastname: Rhu
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
authors.forename: Yong Kee
authors.initials: YK
authors.lastname: Jeong
mesh_terms: D002460:Cell Line; D055453:Coriolaceae / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000096:biosynthesis; D015870:Gene Expression; D005947:Glucose / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability; D009000:Monocytes / Q000187:drug effects; D025282:Mycelium / Q000254:growth & development*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
chemical_list: C493956:CDKN1A protein, human; D003470:Culture Media; D050759:Cyclin-Dependent Kinase Inhibitor p21; D011134:Polysaccharides; D016159:Tumor Suppressor Protein p53; D005947:Glucose