title: Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle.
abstract: The objective of the present study was to identify polymorphisms of the CACNA2D1 gene, and to analyze associations between these polymorphisms and mastitis in several cattle breeds. Through PCR-RFLP methods and DNA sequencing, an allelic variant corresponding to the A→G mutations and Aspartic (Asp) to Glycine (Gly) amino acid replacement at positions 526745 in the exon 25 of bovine CACNA2D1 gene could be detected. Two alleles, A and G, and three genotypes, AA, AG and GG were defined. Genetic character in the studied populations indicated that the A526745G loci of CACNA2D1 gene was moderate polymorphism and fitted with Hardy-Weinberg equilibrium (P > 0.05). The effects of CACNA2D1 polymorphisms on somatic cell score (SCS) were analyzed and significant association was found between A526745G and SCS. The mean of genotype GG was significantly lower than those of genotype AG and AA (P = 0.0469). Information provided in this research could be useful in further studies to determine the role of CACNA2D1 gene in the mastitis resistance.
authors.affiliation: Laboratory of Molecular Biology and Bovine Breeding, The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No.2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. zhengrong_yuan@yahoo.com.cn
authors.forename: Zheng Rong
authors.initials: ZR
authors.lastname: Yuan
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Lu Pei
authors.initials: LP
authors.lastname: Zhang
authors.forename: Li Min
authors.initials: LM
authors.lastname: Zhang
authors.forename: Cui
authors.initials: C
authors.lastname: Chen
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Chen
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Jun Ya
authors.initials: JY
authors.lastname: Li
authors.forename: Jin Bao
authors.initials: JB
authors.lastname: Chen
authors.forename: Hui Jiang
authors.initials: HJ
authors.lastname: Gao
authors.forename: Shang Zhong
authors.initials: SZ
authors.lastname: Xu
mesh_terms: D000818:Animals; D001947:Breeding; D015220:Calcium Channels / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D056426:Genetic Loci; D005838:Genotype; D008892:Milk / Q000166:cytology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D015220:Calcium Channels