title: Molecular markers for identification of Stellantchasmus falcatus and a phylogenic study using the HAT-RAPD method.
abstract: Stellantchasmus falcatus is a minute intestinal fluke in the family Heterophyidae. Metacercariae, the infective stage, were reported in a marine fish, mullet Liza subviridis, and a fresh water fish, Dermogenus pusillus, in Thailand. Adults were found in chicks, rats, cats, and humans. Morphological studies were done for comparing Stellantchasmus sp. worms found in 2 different fish hosts; their shapes and organ arrangements were very similar except for the prepharynx length. Therefore, the present study aimed to compare their DNA fingerprints using the HAT-RAPD method for both types of Stellantchasmus and several other related species. Ten arbitrarily selected primers (OPA-04, OPA-09, OPN-02, OPN-03, OPN-09, OPN-12, OPP-11, OPR-15, OPX-13, and OPAD-01) were used. It was found that OPA-09, OPN-03, and OPAD-01 were able to generate S. falcatus specific fragments in mullets which consisted of 200, 760, and 280 bp, respectively. In addition, the results of morphologic, DNA fingerprinting, and phylogenetic analyses strongly suggest that the fresh water and marine specimens of Stellantchamus may be different species.
authors.affiliation: Department of Biology, Chiang Mai University, Chiang Mai 50200, Thailand. cwongsawd@yahoo.com
authors.forename: Chalobol
authors.initials: C
authors.lastname: Wongsawad
authors.forename: Pheravut
authors.initials: P
authors.lastname: Wongsawad
mesh_terms: D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D006578:Heterophyidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D013785:Thailand
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth