title: Cytokine gene polymorphism and Chlamydia trachomatis-specific immune responses.
abstract: Chlamydia trachomatis-induced fallopian tube damage leading to tubal factor infertility (TFI) is linked with TNF, IL-10, and probably IFNG gene polymorphisms. The aim of this study was to clarify the contribution of these cytokine gene polymorphisms to interindividual variation in C trachomatis-specific immune responses and the cross-regulation of secreted cytokines and single nucleotide polymorphisms (SNPs). Cytokine polymorphisms (IL-10 -1082A/G, -819T/C, and -592A/C, IFNG +874T/A, and TNF -308G/A) were genotyped by polymerase chain reaction in 139 women. C trachomatis-specific immune responses were measured using lymphocyte proliferation (LP) induced by C trachomatis E and F strains and chlamydial heat shock protein 60 antigens. Cytokine secretion was measured in culture supernatants of infected and uninfected mononuclear leukocytes. IL-10 -1082/-819/-592 and IFNG +874 SNPs were associated with the intensity of LP responses to C trachomatis antigens. These cytokines also interact with each other and a cumulative effect of IL-10 -1082 and IFNG +874 genotypes was seen in LP responses to C trachomatis antigens. Our data suggest that interleukin-10 and interferon-γ regulate C trachomatis-specific immune responses in humans and that genetic variation in the expression of their coding genes explains interindividual variation in host immune responses to C trachomatis infection.
authors.affiliation: National Institute for Health and Welfare, Oulu, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Ohman
authors.forename: A
authors.initials: A
authors.lastname: Tiitinen
authors.forename: M
authors.initials: M
authors.lastname: Halttunen
authors.forename: J
authors.initials: J
authors.lastname: Paavonen
authors.forename: H-M
authors.initials: HM
authors.lastname: Surcel
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology; D002690:Chlamydia Infections / Q000235:genetics* / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology*; D005184:Fallopian Tube Diseases / Q000276:immunology / Q000382:microbiology; D005260:Female; D015870:Gene Expression; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007247:Infertility, Female / Q000235:genetics* / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D018655:Lymphocyte Count; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma