title: [Application of BRL conditioned medium in ES cells culture].
abstract: OBJECTIVE
  To study the method of using BRL conditioned medium in ES cells culture.
  
  
  METHODS
  ES cells were cultured in BRL conditioned medium, MEF feeder layer, and BRL conditioned medium plus MEF feeder layer respectively. Both the numbers of ES cells and embryonic bodies and the differentiated cells around the ES cell colonies at certain stages were studied to find the differences of growing speed of ES cells in three culture mediums.
  
  
  RESULTS
  On promoting the growth and maintaining the undifferentiated state of ES cells at the early stage after recovery, the effects of BRL conditioned medium were less than those of MEF feeder layer and those of BRL conditioned medium plus MEF feeder layer (P < 0.01), and there was no difference between MEF feeder layer and BRL conditioned medium plus MEF feeder layer (P > 0.05).
  
  
  CONCLUSIONS
  ES cells should be cultivated on MEF feeder layer or in BRL conditioned medium plus MEF feeder layer at the early stage after recovering.
authors.affiliation: Institute of Cardiovascular Diseases, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China.
authors.forename: Q T
authors.initials: QT
authors.lastname: Zeng
authors.forename: W Q
authors.initials: WQ
authors.lastname: Zhu
authors.forename: L S
authors.initials: LS
authors.lastname: Cao
authors.forename: R Z
authors.initials: RZ
authors.lastname: Qin
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhang
authors.forename: J Q
authors.initials: JQ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D053595:Embryonic Stem Cells / Q000166:cytology*; D022781:Hepatocytes; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011915:Rats, Inbred BUF
chemical_list: D017077:Culture Media, Conditioned