title: Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.
abstract: The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovského nám. 2, 162 06 Praha 6, Czech Republic. t.skalova@gmail.com
authors.forename: Tereza
authors.initials: T
authors.lastname: Skálová
authors.forename: Jarmila
authors.initials: J
authors.lastname: Dušková
authors.forename: Jindřich
authors.initials: J
authors.lastname: Hašek
authors.forename: Andrea
authors.initials: A
authors.lastname: Stěpánková
authors.forename: Tomáš
authors.initials: T
authors.lastname: Koval
authors.forename: Lars Henrik
authors.initials: LH
authors.lastname: Østergaard
authors.forename: Jan
authors.initials: J
authors.lastname: Dohnálek
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003116:Color; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D005292:Ferricyanides / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D048372:Streptomyces coelicolor / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D005292:Ferricyanides; D003300:Copper; D007501:Iron; D042845:Laccase; C028033:potassium ferricyanide