title: Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules.
abstract: Cyclin A is a member of the cyclin family of proteins, which are required for both the mitotic and meiotic divisions that characterise spermatogenesis in human and other mammalian species. The data on cyclin A expression in various human spermatogenic disorders and its relationship to the morphology of seminiferous tubules are not well clarified. This study aimed to evaluate the immunohistochemical expression of cyclin A in testicular biopsies of different spermatogenic disorders correlating with the morphology of seminiferous tubules using morphometry tools. Immunohistochemical evaluation of cyclin A was carried out on testicular biopsies obtained from 48 infertile males (nonobstructive azoospermia) and 15 normal subjects together with using semiautomatic morphometric analysis for evaluation of seminiferous tubules. Cyclin A is expressed in 100% of normal and hypospermatogenesis groups and in 80% of maturation arrest group, with complete absence in Sertoli cell only group. In positive cases, cyclin A stained the nuclei of spermatogonia and primary spermatocytes with a higher intensity of expression in normal cases compared with infertile group. Cyclin A expression was significantly associated with the different examined morphometric parameters. Cyclin A is involved in both mitosis and meiosis of human spermatogenesis as it is expressed in spermatogonia and primary spermatocytes. Morphometry of human testis is intimately correlated with the testicular histopathology.
authors.affiliation: Pathology Department, Menofiya University, Shebein Elkom, Egypt. Asmaa_elsaidy@yahoo.com
authors.forename: A G
authors.initials: AG
authors.lastname: Abdou
authors.forename: M A
authors.initials: MA
authors.lastname: Hammam
authors.forename: A G A
authors.initials: AG
authors.lastname: Farag
authors.forename: S
authors.initials: S
authors.lastname: Farouk
authors.forename: M
authors.initials: M
authors.lastname: Fawzy
mesh_terms: D000328:Adult; D001706:Biopsy; D016022:Case-Control Studies; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D019925:Cyclin A / Q000378:metabolism*; D005298:Fertility / Q000502:physiology*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008540:Meiosis / Q000502:physiology; D008938:Mitosis / Q000502:physiology; D012189:Retrospective Studies; D012671:Seminiferous Tubules / Q000473:pathology*; D013090:Spermatocytes / Q000378:metabolism / Q000473:pathology; D013091:Spermatogenesis / Q000502:physiology; D013093:Spermatogonia / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000378:metabolism* / Q000473:pathology*
chemical_list: D019925:Cyclin A