title: Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration.
abstract: Confocal fluorescence ratiometric imaging is an optical technique used to measure a variety of important biological parameters. A small amount of chromatic aberration in the microscope system can introduce a variation in the signal ratio dependent on the fluorophore concentration gradient along the optical axis and lead to bias in the measurement. We present a theoretical model of this effect. Experimental results and simulations clearly demonstrate that this error can be significant and should not be ignored.
authors.affiliation: College of Optical Sciences, University of Arizona, 1630 East University Boulevard, Tucson, Arizona 85721, USA.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Lin
authors.forename: Arthur F
authors.initials: AF
authors.lastname: Gmitro
mesh_terms: D000465:Algorithms; D003116:Color; D003198:Computer Simulation; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008962:Models, Theoretical; D019047:Phantoms, Imaging