title: Convergent evolution-guided design of antimicrobial peptides derived from influenza A virus hemagglutinin.
abstract: Antimicrobial activity and solution structures of four 13-amino acid peptides derived from the fusion domain of viral hemagglutinin proteins are presented. The results show that carboxyl-terminal amidation is a key factor to switch a viral fusion domain-derived sequence into an antimicrobial peptide. Optimization of amphiphilic balance on the amidated analogue largely improves efficacy and enlarges antimicrobial spectra of these peptides. Our work indicates that viral fusion domains have potential to be engineered into potent antimicrobial peptides.
authors.affiliation: Group of Animal Innate Immunity, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China. Zhusy@ioz.ac.cn
authors.forename: Shunyi
authors.initials: S
authors.lastname: Zhu
authors.forename: André
authors.initials: A
authors.lastname: Aumelas
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry*; D006461:Hemolysis; D053122:Influenza A Virus, H3N2 Subtype / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013329:Structure-Activity Relationship; D014760:Viral Fusion Proteins / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014760:Viral Fusion Proteins