title: Mediterranean glucose-6-phosphate dehydrogenase (G6PD(C563T)) mutation among Jordanian females with acute hemolytic crisis.
abstract: OBJECTIVE
  To evaluate the G6PD(C563T) Mediterranean mutation among Jordanian females who were admitted to Princess Rahma Teaching Hospital (PRTH) with/or previous history of favism.
  
  
  METHODS
  A descriptive study.
  
  
  METHODS
  Jordanian University of Science and Technology and PRTH, from October 2003 to October 2004.
  
  
  METHODS
  After obtaining approval from the Ethics Committee of Jordanian University of Science and Technology, a total of 32 females were included in this study. Samples from 15 healthy individual females were used as a negative control. Blood samples from these patients were collected and analyzed by allele-specific polymerase chain reaction (AS-PCR) to determine the G6PD(C563T) mutation.
  
  
  RESULTS
  Twenty one out of 32 patients were found to be G6PD(C563T) Mediterranean mutation (65.6%) positive. Three out of 21 patients were homozygous and remaining 18 were heterozygous for G6PD(C563T) Mediterranean mutation. Eleven (34.4%) out of 32 patients were found to be negative for G6PD(C563T) mutation indicating the presence of other G6PD mutations in the study sample.
  
  
  CONCLUSIONS
  G6PD(C563T) Mediterranean mutation accounted for 65.6% of the study sample with favism in the North of Jordan. There is likely to be another G6PD deficiency variant implicated in acute hemolytic crisis (favism).
authors.affiliation: Department of Haematolgy, Advanced Medical and Dental Institute (AMDI), University Sains Malaysia, Malaysia.
authors.forename: Abdullah Abdul Jabbar Ahmed
authors.initials: AA
authors.lastname: Al-Alimi
authors.forename: Naeem
authors.initials: N
authors.lastname: Kanakiri
authors.forename: Muhammad
authors.initials: M
authors.lastname: Kamil
authors.forename: Hala Saleh
authors.initials: HS
authors.lastname: Al-Rimawi
authors.forename: Abdul Hamid
authors.initials: AH
authors.lastname: Zaki
authors.forename: Narazah Mohammad
authors.initials: NM
authors.lastname: Yusoff
mesh_terms: D000208:Acute Disease; D005236:Favism / Q000150:complications / Q000235:genetics*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000150:complications / Q000235:genetics*; D006461:Hemolysis / Q000235:genetics*; D006801:Humans; D007597:Jordan; D009154:Mutation
chemical_list: D005954:Glucosephosphate Dehydrogenase