title: Sequence information on simple sequence repeats and single nucleotide polymorphisms through transcriptome analysis of mungbean.
abstract: Mungbean (Vigna radiata (L.) Wilczek) is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs (≥ 500 bp) with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat (SSR) motifs was identified in Jangan (1 630) compared with that of Sunhwa (1 334). A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms (SNPs) and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was approximately 860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.
authors.affiliation: Department of Palnt Resources, College of Industrial Sciences, Kongju National University, Yesan 304-702, Republic of Korea.
authors.forename: Kyaw Thu
authors.initials: KT
authors.lastname: Moe
authors.forename: Jong-Wook
authors.initials: JW
authors.lastname: Chung
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Cho
authors.forename: Jung-Kyung
authors.initials: JK
authors.lastname: Moon
authors.forename: Ja-Hwan
authors.initials: JH
authors.lastname: Ku
authors.forename: Jin-Kyo
authors.initials: JK
authors.lastname: Jung
authors.forename: Jungran
authors.initials: J
authors.lastname: Lee
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Park
mesh_terms: D007887:Fabaceae / Q000235:genetics*; D020869:Gene Expression Profiling; D018598:Minisatellite Repeats; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA