title: Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.
abstract: ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod-shaped crystals belonged to space group P2(1)22, with unit-cell parameters a=66.7, b=131.7, c=224.6 Å, α=90, β=90, γ=90°. Data were collected to 2.9 Å resolution. A preliminary molecular-replacement solution implied the presence of two ElaGT molecules in the asymmetric unit.
authors.affiliation: National Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, 190 Kaiyuan Avenue, Guangzhou Science Park, Guangzhou 510530, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Gong
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Sumei
authors.initials: S
authors.lastname: Li
authors.forename: Changsheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry* / Q000235:genetics; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D018942:Macrolides; C033279:elaiophylin; D016695:Glycosyltransferases