title: The pericentriolar satellite protein CEP90 is crucial for integrity of the mitotic spindle pole.
abstract: Pericentriolar satellites are electron-dense granules that are concentrated around the centrosome. They are involved in the recruitment of centrosomal proteins and microtubule organization in interphase cells, but their mitotic functions are largely unknown. In this study, we characterize CEP90 as a component of pericentriolar satellites. CEP90 is present both in the centrosome and in the cytoplasm, but is transiently concentrated at the centrosome once cells enter mitosis. Depletion of CEP90 caused mitotic arrest with misaligned chromosomes. Spindle pole fragmentation was the most characteristic phenotype in CEP90-depleted cells. Spindle poles were fragmented as soon as the spindles attached, suggesting that the mechanical forces of spindle microtubules physically stress the structure of CEP90-depleted spindle poles. Based on these results, we propose that CEP90 is crucial for maintaining the integrity of spindle poles during mitosis.
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Kyeongmi
authors.initials: K
authors.lastname: Kim
authors.forename: Kunsoo
authors.initials: K
authors.lastname: Rhee
mesh_terms: D002460:Cell Line; D018385:Centrosome / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000502:physiology; D034622:RNA Interference; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone