title: Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase.
abstract: Based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the Sphingomyelinase-D (SMase-D) activity of scorpion, spider and snake venoms by monitoring spectrophotometrically the product of oxidation of HRP released. The results indicate that Loxosceles crude venoms (Loxosceles intermedia, Loxosceles laeta, Loxosceles gaucho and Loxosceles similis) displayed SMase-D activity in a concentration-dependent manner. Furthermore, this activity was blocked by the anti-loxoscelic antivenom. However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity.
authors.affiliation: Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
authors.forename: Marco Túlio R
authors.initials: MT
authors.lastname: Gomes
authors.forename: Gabriela
authors.initials: G
authors.lastname: Guimarães
authors.forename: Frédéric
authors.initials: F
authors.lastname: Frézard
authors.forename: Evanguedes
authors.initials: E
authors.lastname: Kalapothakis
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Minozzo
authors.forename: Olga Meiri
authors.initials: OM
authors.lastname: Chaim
authors.forename: Silvio Sanches
authors.initials: SS
authors.lastname: Veiga
authors.forename: Sergio Costa
authors.initials: SC
authors.lastname: Oliveira
authors.forename: Carlos
authors.initials: C
authors.lastname: Chávez-Olórtegui
mesh_terms: D000818:Animals; D000997:Antivenins / Q000494:pharmacology; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011994:Recombinant Proteins; D013045:Species Specificity; D013109:Sphingomyelins / Q000737:chemistry / Q000378:metabolism; D013111:Spider Venoms / Q000201:enzymology* / Q000633:toxicity; D013112:Spiders / Q000145:classification
chemical_list: D000997:Antivenins; D008081:Liposomes; D011994:Recombinant Proteins; D013109:Sphingomyelins; D013111:Spider Venoms; D002784:Cholesterol; D006735:Horseradish Peroxidase; D010727:Phosphoric Diester Hydrolases; C055160:sphingomyelin phosphodiesterase D