title: High-resolution mass spectrometric analysis of the secretome from mouse lung endothelial progenitor cells.
abstract: Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogenesis.
authors.affiliation: Department of Gene Regulation, HZI, Build. D, Inhoffenstr. 7, 38124, Braunschweig, Germany. katharina.hemmen@ewetel.net
authors.forename: Katherina
authors.initials: K
authors.lastname: Hemmen
authors.forename: Tobias
authors.initials: T
authors.lastname: Reinl
authors.forename: Kerstin
authors.initials: K
authors.lastname: Buttler
authors.forename: Friederike
authors.initials: F
authors.lastname: Behler
authors.forename: Hauke
authors.initials: H
authors.lastname: Dieken
authors.forename: Lothar
authors.initials: L
authors.lastname: Jänsch
authors.forename: Jörg
authors.initials: J
authors.lastname: Wilting
authors.forename: Herbert A
authors.initials: HA
authors.lastname: Weich
mesh_terms: D000818:Animals; D017077:Culture Media, Conditioned / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008168:Lung / Q000166:cytology*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D015203:Reproducibility of Results; D012995:Solubility; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D020543:Proteome; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2