title: Hollow mesoporous silica nanoparticles for intracellular delivery of fluorescent dye.
abstract: In this study, hollow mesoporous silica nanoparticles (HMSNs) were synthesized using the sol-gel/emulsion approach and its potential application in drug delivery was assessed. The HMSNs were characterized, by transmission electron microscopy (TEM), Scanning Electron Microscopy (SEM), nitrogen adsorption/desorption and Brunauer-Emmett-Teller (BET), to have a mesoporous layer on its surface, with an average pore diameter of about 2 nm and a surface area of 880 m2/g. Fluorescein isothiocyanate (FITC) loaded into these HMSNs was used as a model platform to assess its efficacy as a drug delivery tool. Its release kinetic study revealed a sequential release of FITC from the HMSNs for over a period of one week when soaked in inorganic solution, while a burst release kinetic of the dye was observed just within a few hours of soaking in organic solution. These FITC-loaded HMSNs was also found capable to be internalized by live human cervical cancer cells (HeLa), wherein it was quickly released into the cytoplasm within a short period of time after intracellular uptake. We envision that these HMSNs, with large pores and high efficacy to adsorb chemicals such as the fluorescent dye FITC, could serve as a delivery vehicle for controlled release of chemicals administered into live cells, opening potential to a diverse range of applications including drug storage and release as well as metabolic manipulation of cells.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, The People's Republic of China. shiqisun21@hotmail.com.
authors.forename: Huichen
authors.initials: H
authors.lastname: Guo
authors.forename: Haisheng
authors.initials: H
authors.lastname: Qian
authors.forename: Shiqi
authors.initials: S
authors.lastname: Sun
authors.forename: Dehui
authors.initials: D
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Zaixin
authors.initials: Z
authors.lastname: Liu
authors.forename: Jinyan
authors.initials: J
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu