title: Synergistic production of L-arabinose from arabinan by the combined use of thermostable endo- and exo-arabinanases from Caldicellulosiruptor saccharolyticus.
abstract: The optimum conditions for the production of L-arabinose from debranched arabinan were determined to be pH 6.5, 75°C, 20 g l(-1) debranched arabinan, 42 Um l(-1) endo-1,5-α-L-arabinanase, and 14 U ml(-1) α-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus and the conditions for sugar beet arabinan were pH 6.0, 75°C, 20 g l(-1) sugar beet arabinan, 3 U ml(-1) endo-1,5-α-L-arabinanase, and 24 U ml(-1) α-L-arabinofuranosidase. Under the optimum conditions, 16 g l(-1)l-arabinose was obtained from 20 g l(-1) debranched arabinan or sugar beet arabinan after 120 min, with a hydrolysis yield of 80% and a productivity of 8 g l(-1)h(-1). This is the first reported trial for the production of L-arabinose from the hemicellulose arabinan by the combined use of endo- and exo-arabinanases.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Yu-Ri
authors.initials: YR
authors.lastname: Lim
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Yeom
authors.forename: Young-Su
authors.initials: YS
authors.lastname: Kim
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001089:Arabinose / Q000096:biosynthesis*; D027461:Beta vulgaris / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D016988:Gram-Positive Asporogenous Rods, Irregular / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000378:metabolism*
chemical_list: D011134:Polysaccharides; C030080:araban; D001089:Arabinose; D006026:Glycoside Hydrolases