title: Cocaine analog coupled to disrupted adenovirus: a vaccine strategy to evoke high-titer immunity against addictive drugs.
abstract: Based on the concept that anticocaine antibodies could prevent inhaled cocaine from reaching its target receptors in the brain, an effective anticocaine vaccine could help reverse cocaine addiction. Leveraging the knowledge that E1(-)E3(-) adenovirus (Ad) gene transfer vectors are potent immunogens, we have developed a novel vaccine platform for addictive drugs by covalently linking a cocaine analog to the capsid proteins of noninfectious, disrupted Ad vector. The Ad-based anticocaine vaccine evokes high-titer anticocaine antibodies in mice sufficient to completely reverse, on a persistent basis, the hyperlocomotor activity induced by intravenous administration of cocaine.
authors.affiliation: Department of Genetic Medicine, Weill Cornell Medical College, New York, New York 10065, USA.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Hicks
authors.forename: Bishnu P
authors.initials: BP
authors.lastname: De
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Rosenberg
authors.forename: Jesse T
authors.initials: JT
authors.lastname: Davidson
authors.forename: Amira Y
authors.initials: AY
authors.lastname: Moreno
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
authors.forename: Sunmee
authors.initials: S
authors.lastname: Wee
authors.forename: George F
authors.initials: GF
authors.lastname: Koob
authors.forename: Neil R
authors.initials: NR
authors.lastname: Hackett
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Kaminsky
authors.forename: Stefan
authors.initials: S
authors.lastname: Worgall
authors.forename: Miklos
authors.initials: M
authors.lastname: Toth
authors.forename: Jason G
authors.initials: JG
authors.lastname: Mezey
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Crystal
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000906:Antibodies / Q000097:blood; D003042:Cocaine / Q000031:analogs & derivatives* / Q000276:immunology* / Q000378:metabolism; D003673:Defective Viruses / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D008124:Locomotion / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects / Q000276:immunology; D019966:Substance-Related Disorders / Q000276:immunology / Q000517:prevention & control; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000906:Antibodies; D014612:Vaccines; D003042:Cocaine