title: Aldehyde-functionalized benzenediazonium cation for multiprobe immobilization on microelectrode array surfaces.
abstract: We report in situ generation of aldehyde-functionalized benzenediazonium cation (ABD) and its use as a suitable linker molecule for fast and selective immobilization of biomolecules on indium-tin-oxide (ITO) electrode surfaces. We prepared ABD through a new reaction procedure, a simultaneous diazotation of the amine group and deprotection of the aldehyde group from an aniline derivative, 2-(4-aminophenyl)-1,3-dithiane, which was revealed on the ITO electrode surfaces through the electrodeposition of the reaction product and the characterization of the resulting surfaces with cyclic voltammetry, X-ray photoelectron spectroscopy, and protein immobilization. We also showed that successive electrodeposition of ABD and probe molecules on individually addressable microarray electrode surfaces can provide a useful platform for efficient detection of multianalyte. The usage of ABD has been demonstrated by the patterning of three different probe molecules on a single substrate and the simultaneous detection of two target molecules.
authors.affiliation: Department of Chemistry, University of Incheon, Incheon 406-772, Korea.
authors.forename: Al-Monsur Jiaul
authors.initials: AM
authors.lastname: Haque
authors.forename: Kyuwon
authors.initials: K
authors.lastname: Kim
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D002412:Cations / Q000737:chemistry*; D003979:Diazonium Compounds / Q000737:chemistry*; D008839:Microelectrodes; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D000447:Aldehydes; D002412:Cations; D003979:Diazonium Compounds; D017971:Tin Compounds; C035176:benzenediazonium; C109984:indium tin oxide