title: [Inhibition and significance of pigment epithelium-derived factor in the development and metastasis of prostate cancer].
abstract: OBJECTIVE
  To study the inhibition and significance of pigment epithelium-derived factor (PEDF) in the development and metastasis of prostate cancer.
  
  
  METHODS
  The expression of PEDF was examined in the normal prostate tissue, benign prostatic hyperplasia, prostate cancer tissue and prostate cancer cell lines, PC-3 and Lncap by immunohistochemical SP method and Western blot. In combination with clinical data, statistical analysis was performed to evaluate the relation of the expression level of PEDF in prostate cancer and the relationship between different histological grades of prostate cancer.
  
  
  RESULTS
  In normal prostate tissue and benign prostate tissue, the expression of PEDF were elevated and it was far higher than the prostate cancer and prostate cancer cell line. The expression of PEDF and the pathological grade of prostate cancer were related to the differentiated carcinoma of prostate tissue, and the expression level of PEDF in poorly differentiated carcinoma below the highly differentiated carcinoma of prostate cancer. In metastatic prostate cancer, the expression of PEDF was lower than that of prostate cancer without metastasis (12% vs 43.1%).
  
  
  CONCLUSIONS
  The expression of PEDF and the incidence of prostate cancer have a negative correlation. The lower grade of prostate cancer cells, the less the tissue expression of PEDF and the higher tendency of invasion and metastasis.
authors.affiliation: Department of Urology, Sun Yat-sen University, Guangzhou 510120, China. guozhenghui@yahoo.com.cn
authors.forename: Zheng-hui
authors.initials: ZH
authors.lastname: Guo
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: DU
authors.forename: Ke-wei
authors.initials: KW
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Jie-qing
authors.initials: JQ
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Dong
authors.forename: You-sheng
authors.initials: YS
authors.lastname: Yao
authors.forename: Tian-xin
authors.initials: TX
authors.lastname: Lin
authors.forename: Wen-lian
authors.initials: WL
authors.lastname: Xie
authors.forename: Chun
authors.initials: C
authors.lastname: Jiang
authors.forename: Jin-li
authors.initials: JL
authors.lastname: Han
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009414:Nerve Growth Factors / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015843:Serpins / Q000378:metabolism*; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor