title: A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon.
abstract: We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Murata
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: A
authors.initials: A
authors.lastname: Lezhava
authors.forename: H
authors.initials: H
authors.lastname: Kinashi
mesh_terms: D001483:Base Sequence; D007446:Chromosome Inversion; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D018626:Retroelements; D017422:Sequence Analysis, DNA; D013305:Streptomyces griseus / Q000235:genetics*; D045564:Transposon Resolvases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D018626:Retroelements; C473491:Tn3 resolvase; D045564:Transposon Resolvases