title: A kaleidoscopic view of the Arabidopsis core cell cycle interactome.
abstract: Although protein-protein interaction (PPI) networks have been shown to offer a systems-wide view of cellular processes, only a few plant PPI maps are available. Recently, the core cell cycle of Arabidopsis thaliana has been analyzed by three independent PPI technologies, including yeast two-hybrid systems, bimolecular fluorescence complementation and tandem affinity purification. Here, we merge the three interactomes with literature-curated and computationally predicted interactions, paving the way for a comprehensive picture of the plant core cell cycle machinery. Platform-specific interactions unveil the strengths and weaknesses of each detection method and give insights into the nature of the interactions among cell cycle proteins. Moreover, comparison of the obtained data reveals that a complete interactome can only be obtained when multiple techniques are applied in parallel.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium. lieven.deveylder@psb.vib-ugent.be
authors.forename: Jelle
authors.initials: J
authors.lastname: Van Leene
authors.forename: Joanna
authors.initials: J
authors.lastname: Boruc
authors.forename: Geert
authors.initials: G
authors.lastname: De Jaeger
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova
authors.forename: Lieven
authors.initials: L
authors.lastname: De Veylder
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D008856:Microscopy, Fluorescence / Q000379:methods; D025941:Protein Interaction Mapping / Q000379:methods; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins