title: Comparative proteome analysis of Bacillus anthracis with pXO1 plasmid content.
abstract: Bacillus anthracis the causative agent of anthrax, is an important pathogen among the Bacillus cereus group of species because of its physiological characteristics and its importance as a biological warfare agent. Tripartite anthrax toxin proteins and a poly-D-glutamic acid capsule are produced by B. anthracis vegetative cells during mammalian hosts infection and when cultured in conditions that are thought to mimic the host environment. To identify the factors regulating virulence in B. anthracis the whole cell proteins were extracted from two B. anthracis strains and separated by narrow range immobilized pH gradient (IPG) strips (pH 4-7), followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins that were differentially expressed were identified by the peptide fingerprinting using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). A total of 23 proteins were identified as being either upregulated or downregulated in the presence or absence of the virulence plasmid pXO1. Two plasmid encoded proteins and 12 cellular proteins were identified and documented as potential virulence factors.
authors.affiliation: Division of Molecular and Life Sciences, Hanyang University, Ansan, 426-791, Republic of Korea.
authors.forename: Sudipto
authors.initials: S
authors.lastname: Shahid
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Hyung Tae
authors.initials: HT
authors.lastname: Lee
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Kim
authors.forename: Ji Cheon
authors.initials: JC
authors.lastname: Kim
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Dal Mu Ri
authors.initials: DM
authors.lastname: Han
authors.forename: Dong In
authors.initials: DI
authors.lastname: Jeon
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D001408:Bacillus anthracis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D037521:Virulence Factors / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors