title: [Hyperglycemia inhibited taurine transport and taurine transporter gene expression in cultured rat cardiomyocytes].
abstract: OBJECTIVE
  To investigate the alterations of taurine transport, and taurine transporter (TAUT) mRNA by hyperglycemia in cultured rat cardiomyocytes.
  
  
  METHODS
  3H-taurine measured the amount of taurine uptake. TAUT mRNA consents were measured using quantitative RT-PCR.
  
  
  RESULTS
  The cellular uptake amounts of taurine in seven groups increased with incubation time, and near to be saturated after 5 min. The uptake amount of 10, 20, and 30 mmol/L glucose groups was obviously lower than that of the control group (P < 0.05 or P < 0.01). In 30 mmmol/L glucose, taurine release obviously was decreased, as compared with that of the control. Exposure of cells to 10, 20, and 30 mmmol/L glucose decreased taurine uptake in a concentration-dependent fashion. Exposure to hyperglycemia did not affect the Km of the TAUT, but the apparent Vmax were significantly decreased (P < 0.05). In 20 and 30 mmmol/L groups, TAUT mRNA contents of myocardial cells were significantly reduced, as compared with the control group (P < 0.05).
  
  
  CONCLUSIONS
  The data suggests that there are dysfunction of taurine uptake and downregulation of TAUT gene expression by glucose in cultured rat cardiomyocytes.
authors.affiliation: Institute of Cardialvascular Disease, the First Hospital of Beijing University, Beijing 100034, China.
authors.forename: Yan-rong
authors.initials: YR
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.forename: Shu-heng
authors.initials: SH
authors.lastname: Wang
authors.forename: Yong-zheng
authors.initials: YZ
authors.lastname: Pang
authors.forename: Chaoshu
authors.initials: C
authors.lastname: Tang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013654:Taurine / Q000378:metabolism*; D000099247:Taurine Transporters
chemical_list: D005947:Glucose; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D013654:Taurine; D000099247:Taurine Transporters