title: Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow.
abstract: Impairments in axonal dopamine release are associated with neurological disorders such as schizophrenia and attention deficit hyperactivity disorder and pathophysiological conditions promoting drug abuse and obesity. The D2 dopamine autoreceptor (D2-AR) exerts tight regulatory control of axonal dopamine (DA) release through a mechanism suggested to involve K(+) channels. To evaluate the contribution of Kv1 voltage-gated potassium channels of the Shaker gene family to the regulation of axonal DA release by the D2-AR, the present study employed expression analyses, real time measurements of striatal DA overflow, K(+) current measurements and immunoprecipitation assays. Kv1.1, -1.2, -1.3, and -1.6 mRNA and protein were detected in midbrain DA neurons purified by fluorescence-activated cell sorting and in primary DA neuron cultures. In addition, Kv1.1, -1.2, and -1.6 were localized to DA axonal processes in the dorsal striatum. By means of fast scan cyclic voltammetry in striatal slice preparations, we found that the inhibition of stimulation-evoked DA overflow by a D2 agonist was attenuated by Kv1.1, -1.2, and -1.6 toxin blockers. A particular role for the Kv1.2 subunit in the process whereby axonal D2-AR inhibits DA overflow was established with the use of a selective Kv1.2 blocker and Kv1.2 knock-out mice. Moreover, we demonstrate the ability of D2-AR activation to increase Kv1.2 currents in co-transfected cells and its reliance on Gβγ subunit signaling along with the physical coupling of D2-AR and Kv1.2-containing channels in striatal tissue. These findings underline the contribution of Kv1.2 in the regulation of nigrostriatal DA release by the D2-AR and thereby offer a novel mechanism by which DA release is regulated.
authors.affiliation: Department of Pharmacology and Groupe de Recherche sur le Système Nerveux Central, Université de Montréal, Quebec H3C 3J7, Canada.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Fulton
authors.forename: Dominic
authors.initials: D
authors.lastname: Thibault
authors.forename: Jose A
authors.initials: JA
authors.lastname: Mendez
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lahaie
authors.forename: Emanuele
authors.initials: E
authors.lastname: Tirotta
authors.forename: Emiliana
authors.initials: E
authors.lastname: Borrelli
authors.forename: Michel
authors.initials: M
authors.lastname: Bouvier
authors.forename: Bruce L
authors.initials: BL
authors.lastname: Tempel
authors.forename: Louis-Eric
authors.initials: LE
authors.lastname: Trudeau
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000235:genetics / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology; D051663:Kv1.2 Potassium Channel / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D017448:Receptors, Dopamine D2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D018491:Dopamine Agonists; D051663:Kv1.2 Potassium Channel; D017448:Receptors, Dopamine D2; D004298:Dopamine