title: Sensitive chemiluminescence immunoassay by capillary electrophoresis with gold nanoparticles.
abstract: This technical note describes a new chemiluminescence immunoassay hyphenated to capillary electrophoresis (CE-based CL-IA) with gold nanoparticles (AuNPs) technique for biological molecules determination. AuNPs were used as a protein label reagent in the light of its excellent catalytic effect to the CL reaction of luminol and hydrogen peroxide. AuNPs conjugate with antibody (Ab) to form tagged antibody (Ab*), and then Ab* link to antigen (Ag) to produce an Ab*-Ag complex by a noncompetitive immunoreaction. The mixture of the excess Ab* and the Ab*-Ag complex was baseline separated and detected within 5 min under the optimized conditions. This new protocol was evaluated with human immunoglobulin G (IgG) as the target molecule. The calibration curve of IgG was in the range of 0.008-5 μg/mL with a correlation coefficient of 0.995. The detection limit (S/N = 3) of IgG was 1.14 × 10(-3) μg/mL (7.1 pmol/L, 0.39 amol). The proposed AuNPs enhanced CE-based CL-IA method was successfully applied for the quantification of IgG in human sera from patients. It proves that the present method could be developed into a new and sensitive biochemical analysis technique.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, China. liuym9518@sina.com
authors.forename: Yan-ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Liu
authors.forename: Long-fei
authors.initials: LF
authors.lastname: Peng
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Chen
authors.forename: Shu-wei
authors.initials: SW
authors.lastname: Ren
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission
chemical_list: D007074:Immunoglobulin G; D006046:Gold