title: Interference of vaccination against bluetongue virus serotypes 1 and 8 with serological diagnosis of small-ruminant lentivirus infection.
abstract: The effects of the recent vaccinations against bluetongue virus serotype 1 (BTV-1) and BTV-8 in Europe on the reliability of enzyme-linked immunosorbent assays (ELISAs) currently used for diagnosis of small-ruminant lentivirus (SRLV) infection were examined. Primary vaccination against BTV-8 in goats induced an increase in reactivity that did not exceed 3 months in a whole-virus indirect ELISA and a competitive ELISA based on the gp135 glycoprotein. Subsequent BTV-1/8 vaccination extended the time scale of false-positive reactivity for up to 6 months. These results are of relevance for SRLV-monitoring programs.
authors.affiliation: Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Anses Laboratoire de Niort, B.P. 3081, 60 rue de Pied de Fond, F-79012 Niort Cedex, France. stephen.valas@anses.fr
authors.forename: Stephen
authors.initials: S
authors.lastname: Valas
authors.forename: Alain
authors.initials: A
authors.lastname: Le Ven
authors.forename: Benoit
authors.initials: B
authors.lastname: Croise
authors.forename: Maud
authors.initials: M
authors.lastname: Maquigneau
authors.forename: Cécile
authors.initials: C
authors.lastname: Perrin
mesh_terms: D000818:Animals; D001820:Bluetongue virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D015511:Goat Diseases / Q000175:diagnosis* / Q000821:virology*; D006041:Goats; D016086:Lentivirus / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000175:diagnosis / Q000662:veterinary*; D015203:Reproducibility of Results; D013997:Time Factors; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014765:Viral Vaccines