title: Identification of G2607A mutation in EGFR gene with a significative rate in Moroccan patients with nasopharyngeal carcinoma.
abstract: The epidermal growth factor receptor (EGFR) is involved in the regulation of several cellular processes and in the development of many human cancers. Somatic mutations of EGFR at tyrosine kinase domain have been associated with clinical response to tyrosine kinase inhibitors (TKIs) in lung cancer patients. In this study, we evaluated the frequency of point mutations in EGFR for future use of TKI in clinical treatment of nasopharyngeal carcinoma (NPC). Sixty Moroccan patient specimens of NPC were analysed for EGFR mutations in the region delimiting exons 18 and 21 by direct sequencing. Our results showed the absence of mutations in the EGFR kinase domain in these exons in all 60 analysed specimens. Sequence analysis of the EGFR&mdash;TK domain, revealed the presence of (G2607A) polymorphism at exon 20. The genotypes AA and GA were found respectively in 39 (65%) and 16 (26.6%) cases. Statistical analysis showed no difference between the polymorphism and either gender or age of patients. Mutations in EGFR kinase domain are rare events in NPC biopsies, suggesting, that treatment of NPC patients with TKI may not be effective. However, EGFR G2607A polymorphism at exon 20 is frequent in NPC cases and could be associated to clinical response to TKI therapy.
authors.affiliation: Institut Pasteur du Maroc, Casablanca, Maroc.
authors.forename: F
authors.initials: F
authors.lastname: Naji
authors.forename: M
authors.initials: M
authors.lastname: Attaleb
authors.forename: N
authors.initials: N
authors.lastname: Laantri
authors.forename: N
authors.initials: N
authors.lastname: Benchakroun
authors.forename: B
authors.initials: B
authors.lastname: El Gueddari
authors.forename: A
authors.initials: A
authors.lastname: Benider
authors.forename: H
authors.initials: H
authors.lastname: Azeddoug
authors.forename: M M
authors.initials: MM
authors.lastname: Ennaji
authors.forename: M
authors.initials: M
authors.lastname: El Mzibri
authors.forename: M
authors.initials: M
authors.lastname: Khyatti
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics*; D002648:Child; D066246:ErbB Receptors / Q000235:genetics*; D005091:Exons; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009018:Morocco; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000235:genetics*; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000627:therapeutic use; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
chemical_list: D047428:Protein Kinase Inhibitors; D066246:ErbB Receptors