title: Validation of lactic acid bacteria, lactic acid, and acidified sodium chlorite as decontaminating interventions to control Escherichia coli O157:H7 and Salmonella Typhimurium DT 104 in mechanically tenderized and brine-enhanced (nonintact) beef at the purveyor.
abstract: After three different outbreaks were linked to the consumption of nonintact meat products contaminated with Escherichia coli O157:H7, the U.S. Food Safety and Inspection Service published notice requiring establishments producing mechanically tenderized and moisture-enhanced beef products to reassess their respective hazard analysis and critical control point systems, due to potential risk to the consumers. The objective of this study was to validate the use of lactic acid bacteria (LAB), acidified sodium chlorite (ASC), and lactic acid (LA) sprays when applied under a simulated purveyor setting as effective interventions to control and reduce E. coli O157:H7 and Salmonella Typhimurium DT 104 in inoculated U.S. Department of Agriculture (USDA) Choice strip loins (longissimus lumborum muscles) pieces intended for either mechanical blade tenderization or injection enhancement with a brine solution after an aging period of 14 or 21 days at 4.4°C under vacuum. After the mechanical process, translocation of E. coli O157:H7 and Salmonella Typhimurium DT 104 from the surface into the internal muscles occurred at levels between 1.00 and 5.72 log CFU/g, compared with controls. LAB and LA reduced internal E. coli O157:H7 loads up to 3.0 log, while ASC reduced the pathogen 1.4 to 2.3 log more than the control (P < 0.05), respectively. Salmonella Typhimurium DT 104 was also reduced internally 1.3 to 2.8, 1.0 to 2.3, and 1.4 to 1.8 log after application of LAB, LA, and ASC, respectively. The application of antimicrobials by purveyors prior to mechanical tenderization or enhancement of steaks should increase the safety of these types of products.
authors.affiliation: Department of Animal and Food Sciences, Texas Tech University, Box 42141, Lubbock, Texas 79409, USA.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Echeverry
authors.forename: J Chance
authors.initials: JC
authors.lastname: Brooks
authors.forename: Markus F
authors.initials: MF
authors.lastname: Miller
authors.forename: Jesse A
authors.initials: JA
authors.lastname: Collins
authors.forename: Guy H
authors.initials: GH
authors.lastname: Loneragan
authors.forename: Mindy M
authors.initials: MM
authors.lastname: Brashears
mesh_terms: D000818:Animals; D000898:Antibiosis; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004202:Disinfectants / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005511:Food Handling / Q000379:methods*; D019344:Lactic Acid / Q000494:pharmacology; D007778:Lactobacillus / Q000502:physiology; D008461:Meat Products / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development*
chemical_list: D002712:Chlorides; D004202:Disinfectants; D019344:Lactic Acid; C001599:chlorite