title: Identification of CJC-1295, a growth-hormone-releasing peptide, in an unknown pharmaceutical preparation.
abstract: Several peptide drugs are being manufactured illicitly, and in some cases they are being made available to the public before entering or completing clinical trials. At the request of Norwegian police and customs authorities, unknown pharmaceutical preparations suspected to contain peptide drugs are regularly subjected to analysis. In 2009, an unknown pharmaceutical preparation was submitted for analysis by liquid chromatography-high resolution tandem mass spectrometry (LC-HRMS/MS). The preparation was found to contain a 29 amino acid peptide with a C-terminal amide function. Based on the interpretation of mass spectrometric data, an amino acid sequence was proposed. The sequence is consistent with a peptide currently marketed under the name CJC-1295. CJC-1295 is a releasing factor for growth hormone and is therefore considered a Prohibited Substance under Section S2 of the WADA Prohibited List. This substance has potential performance-enhancing effects, it is readily available, and there is reason to believe that it is being used within the bodybuilding community.
authors.affiliation: Norwegian Doping Control Laboratory, Oslo University Hospital. john.henninge@h-lab.no
authors.forename: John
authors.initials: J
authors.lastname: Henninge
authors.forename: Milaim
authors.initials: M
authors.lastname: Pepaj
authors.forename: Ingunn
authors.initials: I
authors.lastname: Hullstein
authors.forename: Peter
authors.initials: P
authors.lastname: Hemmersbach
mesh_terms: D002853:Chromatography, Liquid; D013007:Growth Hormone-Releasing Hormone / Q000031:analogs & derivatives* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification*; D058573:Performance-Enhancing Substances / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: C502076:CJC 1295; D010446:Peptide Fragments; D058573:Performance-Enhancing Substances; D004364:Pharmaceutical Preparations; D013007:Growth Hormone-Releasing Hormone