title: Molecular detection of meat animal species targeting MT 12S rRNA gene.
abstract: The efficacy of PCR-RFLP analysis of mt 12S rRNA gene in identification of animal species from meat samples of known and unknown origin and adulterated meat samples was evaluated. In PCR, all the samples generated an amplicon of 456 bp. Restriction enzyme digestion of the PCR product with AluI, HhaI, BspTI and ApoI revealed characteristic RFLP patterns. Of the samples of unknown origin few were identified as cattle, few as buffalo and some were admixtures of two, suggesting adulteration. The RFLP pattern of one did not match any of species included in the study, which on sequencing was confirmed as camel meat. Application of this technique on adulterated meat samples could detect both animal species in proportion of 50:50 and 75:25 (except in case of goat+cattle). The technique however could not detect any of the two species when proportion of mixture was 90:10 (except in case of cattle+buffalo).
authors.affiliation: Department of Animal Biotechnology, Bombay Veterinary College, Parel, Mumbai 400 012, India. madhu_mahajan@yahoo.co.in
authors.forename: M V
authors.initials: MV
authors.lastname: Mahajan
authors.forename: Y P
authors.initials: YP
authors.lastname: Gadekar
authors.forename: V D
authors.initials: VD
authors.lastname: Dighe
authors.forename: R D
authors.initials: RD
authors.lastname: Kokane
authors.forename: A S
authors.initials: AS
authors.lastname: Bannalikar
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics; D002417:Cattle / Q000235:genetics; D006041:Goats / Q000235:genetics; D008460:Meat / Q000032:analysis* / Q000145:classification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000032:analysis; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000032:analysis*; D013045:Species Specificity
chemical_list: D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D012313:RNA