title: [Effects of genistein on proliferation and differentiation of osteoblast].
abstract: OBJECTIVE
  To study the effects of genistein on proliferation and differentiation of osteoblasts in neonatal rat calvaria cultures.
  
  
  METHODS
  Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the presence of different doses of genistein (10(-5) mol/L, 10(-6) mol/L and 10(-7) mol/L). The proliferation and DNA and collagen synthesis of osteoblasts were assayed by MTT method and 3H-TdR and 3H-proline incorporation. The activity of ALP were measured by ALP assay kit.
  
  
  RESULTS
  Genistein significantly increased osteoblast 3H-TdR and 3H-proline incorporation and MTT, 10(-6) mol/L genistein increased ALP activity.
  
  
  CONCLUSIONS
  Genistein increased osteoblast DNA and collagen synthesis in neonatal rat calvaria cultures, and promoted osteoblast proliferation and differentiation.
authors.affiliation: Institue of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Yue-hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Lan-xing
authors.initials: LX
authors.lastname: Gao
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Wen-kao
authors.initials: WK
authors.lastname: Nan
authors.forename: Xian-yuan
authors.initials: XY
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D019833:Genistein / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003094:Collagen; D004247:DNA; D019833:Genistein