title: Impact of human metapneumovirus infection on in and outpatients for the years 2006-2008 in Southern Brazil.
abstract: The human metapneumovirus (hMPV), member of the Paramyxoviridae family, has been reported as an important agent involved with acute respiratory infections (ARIs). The aim of this study is to identify hMPV as the etiological agent of ARIs on in and outpatients in the city of Curitiba, Southern Brazil, and describe clinical data of hMPV subtyping. A retrospective study was performed in 1,572 respiratory samples over a period of three years. hMPV was detected by reverse transcription-polymerase chain reaction and subtyping was performed by nucleotide sequencing. hMPV was present in 61 (3.9%) samples and subtypes A1, A2a, B1 and B2 were detected. The incidence of hMPV was higher in outpatients (5.9%), whose mean age was 19.7 years (range 6 months-75 years old), than in inpatients (3%), whose mean age was 7.6 months (range 1 month-26 years old). The outpatients had upper respiratory tract infections with flu-like symptoms and all hospitalized children had lower respiratory tract infections. A pediatric patient died from complications associated with hMPV A2a infection. hMPV has been reported as a respiratory pathogen in all age groups. No correlation was observed between viral subtype and disease severity in the samples of this study.
authors.affiliation: Laboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brasil, 82060-240.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Debur
authors.forename: Luine Rr
authors.initials: LR
authors.lastname: Vidal
authors.forename: Elenice
authors.initials: E
authors.lastname: Stroparo
authors.forename: Meri B
authors.initials: MB
authors.lastname: Nogueira
authors.forename: Sérgio M
authors.initials: SM
authors.lastname: Almeida
authors.forename: Gislene A
authors.initials: GA
authors.lastname: Takahashi
authors.forename: Indianara
authors.initials: I
authors.lastname: Rotta
authors.forename: Luciane A
authors.initials: LA
authors.lastname: Pereira
authors.forename: Clyete S
authors.initials: CS
authors.lastname: Silveira
authors.forename: Adriana
authors.initials: A
authors.lastname: Delfraro
authors.forename: Sueli M
authors.initials: SM
authors.lastname: Nakatani
authors.forename: Irene
authors.initials: I
authors.lastname: Skraba
authors.forename: Sonia M
authors.initials: SM
authors.lastname: Raboni
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006760:Hospitalization / Q000706:statistics & numerical data; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D029121:Metapneumovirus / Q000235:genetics*; D008875:Middle Aged; D010045:Outpatients / Q000706:statistics & numerical data; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000821:virology*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult