title: Role of nitric oxide in shiga toxin-2-induced premature delivery of dead fetuses in rats.
abstract: Shiga toxin-producing Escherichia coli (STEC) infections could be one of the causes of fetal morbimortality in pregnant women. The main virulence factors of STEC are Shiga toxin type 1 and/or 2 (Stx1, Stx2). We previously reported that intraperitoneal (i.p.) injection of rats in the late stage of pregnancy with culture supernatant from recombinant E. coli expressing Stx2 and containing lipopolysaccharide (LPS) induces premature delivery of dead fetuses. It has been reported that LPS may combine with Stx2 to facilitate vascular injury, which may in turn lead to an overproduction of nitric oxide (NO). The aim of this study was to evaluate whether NO is involved in the effects of Stx2 on pregnancy. Pregnant rats were i.p. injected with culture supernatant from recombinant E. coli containing Stx2 and LPS (sStx2) on day 15 of gestation. In addition, some rats were injected with aminoguanidine (AG), an inducible isoform inhibitor of NO synthase (iNOS), 24 h before and 4 h after sStx2 injection. NO production was measured by NOS activity and iNOS expression by Western blot analysis. A significant increase in NO production and a high iNOS expression was observed in placental tissues from rats injected with sStx2 containing 0.7 ng and 2 ng Stx2/g body weight and killed 12 h after injection. AG caused a significant reduction of sStx2 effects on the feto-maternal unit, but did not prevent premature delivery. Placental tissues from rats treated with AG and sStx2 presented normal histology that was indistinguishable from the controls. Our results reveal that Stx2-induced placental damage and fetus mortality is mediated by an increase in NO production and that AG is able to completely reverse the Stx2 damages in placental tissues, but not to prevent premature delivery, thus suggesting other mechanisms not yet determined could be involved.
authors.affiliation: Laboratorio de Fisiopatogenia, Departamento de Fisiología, Facultad de Medicina, Buenos Aires, Argentina.
authors.forename: Juliana
authors.initials: J
authors.lastname: Burdet
authors.forename: Elsa
authors.initials: E
authors.lastname: Zotta
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Cella
authors.forename: Ana M
authors.initials: AM
authors.lastname: Franchi
authors.forename: Cristina
authors.initials: C
authors.lastname: Ibarra
mesh_terms: D000818:Animals; D001835:Body Weight; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D008070:Lipopolysaccharides / Q000378:metabolism; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000737:chemistry*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D020033:Protein Isoforms; D051381:Rats; D022641:Shiga Toxin 2 / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D008070:Lipopolysaccharides; D020033:Protein Isoforms; D022641:Shiga Toxin 2; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II