title: Proteomic analysis of swine hepatitis E virus (sHEV)-infected livers reveals upregulation of apolipoprotein and downregulation of ferritin heavy chain.
abstract: Swine hepatitis E virus (sHEV) has been discovered to be almost ubiquitous in pigs, and is antigenically and genetically related to human HEV. Proteomic analysis was used to identify altered protein expression in swine liver, using two-dimensional electrophoresis and peptide mass fingerprinting. A total of 10 protein spots exhibited significant alterations in the sHEV-infected organ. The upregulation of apolipoprotein E (Apo E) and downregulation of ferritin heavy chain were confirmed by Western analysis and by semi-quantitative reverse transcription-PCR. The elevated expression of Apo E may provide a novel insight into molecular responses to HEV infection in swine.
authors.affiliation: Virology Division, National Veterinary Research and Quarantine Service, Anyang, Korea.
authors.forename: Garam
authors.initials: G
authors.lastname: Lee
authors.forename: Dongun
authors.initials: D
authors.lastname: Han
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Sorah
authors.initials: S
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000032:analysis*; D001053:Apolipoproteins / Q000032:analysis*; D015153:Blotting, Western; D015536:Down-Regulation; D016751:Hepatitis E / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D016752:Hepatitis E virus / Q000472:pathogenicity; D006801:Humans; D008099:Liver / Q000473:pathology* / Q000821:virology; D020543:Proteome / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000473:pathology* / Q000821:virology; D015854:Up-Regulation
chemical_list: D001053:Apolipoproteins; D020543:Proteome; D001052:Apoferritins