title: [Effects of cofilin phosphorylation on the actin cytoskeleton reorganization induced by shear stress].
abstract: OBJECTIVE
  To explore the effects of cofilin on the actin cytoskeleton reorganization in osteoblasts induced by fluid shear stress.
  
  
  METHODS
  Fluid shear stress (1.2 Pa) was applied to osteoblasts for 0 (control group), 15, 30, 45, 60, 120 min in vitro. Cells were stained with fluorescein isothiocyanate (FITC)-phalloidin for fiber-actin, and confocal laser scanning microscope(CLSM) was used to observe the fluorescence of fiber-actin. Western blotting was used to detect the expression of the cofilin and the phospho-cofilin.
  
  
  RESULTS
  Actin filaments became organized into stress fibers that were thicker and more abundant than those in non-flowed cells. The fluorescence intensity (38.00 ± 6.88) of fiber-actin after 120 min (42.93 ± 6.41) loading it was 2.8 times as much as that in control group (15.41 ± 3.60, P < 0.05). Additionally, the level of phospho-cofilin protein was dramatically elevated after loading. Fluid shear stress induced an initial decrease of cofilin at 60 min. However, at 120 min cofilin (0.254 ± 0.026) increased to 1.5 times as much as that at 60 min (0.162 ± 0.004).
  
  
  CONCLUSIONS
  The results indicate that cofilin phosphorylation mediates fiber-actin reorganization in the osteoblasts induced by fluid shear stress.
authors.affiliation: Department of Prosthodontics, Sun Yat-sen University, Guangzhou 510055, China.
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Liu
authors.forename: You-rui
authors.initials: YR
authors.lastname: Li
authors.forename: Min-feng
authors.initials: MF
authors.lastname: Shao
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D051339:Actin Depolymerizing Factors / Q000096:biosynthesis*; D006801:Humans; D010006:Osteoblasts / Q000648:ultrastructure*; D010766:Phosphorylation; D013314:Stress, Mechanical
chemical_list: D051339:Actin Depolymerizing Factors