title: Understanding splicing regulation through RNA splicing maps.
abstract: Alternative splicing is a highly regulated process that greatly increases the proteome diversity and plays an important role in cellular differentiation and disease. Interactions between RNA-binding proteins (RBPs) and pre-mRNA are the principle regulator of splicing decisions. Findings from recent genome-wide studies of protein-RNA interactions have been combined with assays of the global effects of RBPs on splicing to create RNA splicing maps. These maps integrate information from all pre-mRNAs regulated by single RBPs to identify the global positioning principles guiding splicing regulation. Recent studies using this approach have identified a set of positional principles that are shared between diverse RBPs. Here, we discuss how insights from RNA splicing maps of different RBPs inform the mechanistic models of splicing regulation.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK, CB2 0QH.
authors.forename: Joshua T
authors.initials: JT
authors.lastname: Witten
authors.forename: Jernej
authors.initials: J
authors.lastname: Ule
mesh_terms: D005786:Gene Expression Regulation / Q000235:genetics; D055106:Genome-Wide Association Study; D006801:Humans; D007438:Introns / Q000235:genetics; D008954:Models, Biological; D012326:RNA Splicing / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins