title: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis.
abstract: The nuclear pore complex (NPC) is the only gateway for molecular trafficking across the nuclear envelope. The NPC is not merely a static nuclear-cytoplasmic transport gate; the functional analysis of nucleoporins has revealed dynamic features of the NPC in various cellular functions, such as mitotic spindle formation and protein modification. However, it is not known whether the NPC undergoes dynamic changes during biological processes such as cell differentiation. In the present study, we evaluate changes in the expression levels of several nucleoporins and show that the amount of Nup358/RanBP2 within individual NPCs increases during muscle differentiation in C2C12 cells. Using atomic force microscopy, we demonstrate structural differences between the cytoplasmic surfaces of myoblast and myotube NPCs and a correlation between the copy number of Nup358 and the NPC structure. Furthermore, small interfering RNA-mediated depletion of Nup358 in myoblasts suppresses myotube formation without affecting cell viability, suggesting that NUP358 plays a role in myogenesis. These findings indicate that the NPC undergoes dynamic remodeling during muscle cell differentiation and that Nup358 is prominently involved in the remodeling process.
authors.affiliation: Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, Japan.
authors.forename: Munehiro
authors.initials: M
authors.lastname: Asally
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Yasuda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Oka
authors.forename: Shotaro
authors.initials: S
authors.lastname: Otsuka
authors.forename: Shige H
authors.initials: SH
authors.lastname: Yoshimura
authors.forename: Kunio
authors.initials: K
authors.lastname: Takeyasu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoneda
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D051379:Mice; D018625:Microscopy, Atomic Force; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism*; D032446:Myoblasts / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C094493:ran-binding protein 2