title: Molecular mechanisms of the effect of interleukin-2 on apoptosis of blood lymphocytes.
abstract: The effects of recombinant IL-2 on apoptosis of lymphocytes of healthy donors were studied in in vitro experiments. It was shown that the inductive and inhibitory effects of IL-2 on apoptotic process depend on the dose of the cytokine and cell microenvironmental conditions. Culturing of lymphocytes with recombinant IL-2 increases the percent of cells with reduced transmembrane potential, reduces the content of intracellular proteins Bcl-2, Bcl-X(L) and и Bax, and increases the level of Bad. The proapoptotic effect of this cytokine is realized with participation of nuclear transcription factor NF-κB and transcription factor P53.
authors.affiliation: Department of Fundamentals of Clinical Medicine, Scientific and Educational Center of Molecular Medicine, Siberian State Medical University, Russian Ministry of Health Care and Social Development, Tomsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Ryazantseva
authors.forename: V V
authors.initials: VV
authors.lastname: Novitskii
authors.forename: O B
authors.initials: OB
authors.lastname: Zhukova
authors.forename: E V
authors.initials: EV
authors.lastname: Sazonova
authors.forename: O E
authors.initials: OE
authors.lastname: Chechina
authors.forename: A K
authors.initials: AK
authors.lastname: Biktasova
authors.forename: A N
authors.initials: AN
authors.lastname: Baikov
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: C494819:BAD protein, human; D007376:Interleukin-2; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D051020:bcl-X Protein