title: Raman spectroscopy for the process analysis of the manufacturing of a suspension metered dose inhaler.
abstract: The purpose of this research was to demonstrate the utility of Raman spectroscopy for process analysis of a suspension metered dose inhaler manufacturing process. Chemometric models were constructed for the quantification of ethanol and active pharmaceutical ingredient such that both could be monitored in real-time during the compounding and filling operations via tank measurements and recirculation line flow-cell measurements. Different spectral preprocessing techniques were used to delineate the effects of mixing speed and temperature changes from actual concentration effects. Raman spectroscopy offers advantages in time savings and quality of information over the standard methods of analysis for respiratory formulations, such as a drug content assay via HPLC and ethanol testing via GC. The successful implementation of this work will allow formulation scientists to quantitatively assess both the formulation (e.g., the concentration of active pharmaceutical ingredient (API) and ethanol), as well as the manufacturing process (e.g., determination of mixing endpoints) in real-time.
authors.affiliation: Merck Respiratory Product Development, 556 Morris Avenue, S7-A2-2123, Summit, NJ 07901, United States.
authors.forename: James
authors.initials: J
authors.lastname: Butz
authors.forename: Luis
authors.initials: L
authors.lastname: de la Cruz
authors.forename: Jason
authors.initials: J
authors.lastname: DiTonno
authors.forename: Kevin
authors.initials: K
authors.lastname: DeBoyace
authors.forename: Gary
authors.initials: G
authors.lastname: Ewing
authors.forename: Brent
authors.initials: B
authors.lastname: Donovan
authors.forename: Joseph
authors.initials: J
authors.lastname: Medendorp
mesh_terms: D002138:Calibration; D004339:Drug Compounding / Q000379:methods*; D004867:Equipment Design; D000431:Ethanol / Q000032:analysis; D036501:Metered Dose Inhalers / Q000592:standards*; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013678:Technology, Pharmaceutical / Q000295:instrumentation / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations; D000431:Ethanol