title: Genes for hydrogen peroxide detoxification and adaptation contribute to protection against heat shock in Xanthomonas campestris pv. campestris.
abstract: Xanthomonas campestris pv. campestris, a soil-borne plant-pathogenic bacterium, is exposed to multiple stresses in the environment and during interaction with a host plant. The roles of hydrogen peroxide (H(2) O(2) )-protective genes (katA, katG, and ahpC) and a peroxide sensor/transcription regulator (oxyR) in the viability of X. campestris pv. campestris at an elevated temperature were evaluated. The single katA and katG mutants showed moderate decreased survival after the heat treatment, while the double katA-katG and oxyR mutants were the most vulnerable to the heat treatment compared with a wild-type strain. However, ahpC provided no protective function against the heat treatment. Flow cytometric analysis revealed an increased accumulation of peroxide in cells treated with heat. Altogether, the data revealed a crucial role of genes in the H(2) O(2) detoxification system for protection against lethal heat shock in X. campestris pv. campestris.
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Sarinya
authors.initials: S
authors.lastname: Buranajitpakorn
authors.forename: Anong
authors.initials: A
authors.lastname: Piwkam
authors.forename: Nisanart
authors.initials: N
authors.lastname: Charoenlap
authors.forename: Paiboon
authors.initials: P
authors.lastname: Vattanaviboon
authors.forename: Skorn
authors.initials: S
authors.lastname: Mongkolsuk
mesh_terms: D001711:Biotransformation; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D050296:Microbial Viability / Q000187:drug effects* / Q000528:radiation effects*; D016877:Oxidants / Q000378:metabolism / Q000633:toxicity; D018384:Oxidative Stress; D013312:Stress, Physiological; D016959:Xanthomonas campestris / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology* / Q000528:radiation effects
chemical_list: D016877:Oxidants; D006861:Hydrogen Peroxide