title: Electrophoretic fabrication of chitosan-zirconium-oxide nanobiocomposite platform for nucleic acid detection.
abstract: The present work describes electrophoretic fabrication of nanostructured chitosan-zirconium-oxide composite (CHIT-NanoZrO(2)) film (180 nm) onto indium-tin-oxide (ITO)-coated glass plate. This nanobiocomposite film has been explored as immobilization platform for probe DNA specific to M. Tuberculosis as model biomolecule to investigate its sensing characteristics. It is revealed that pH-responsive behavior of CHIT and its cationic skeleton is responsible for the movement of CHIT-NanoZrO(2) colloids toward cathode during electrophoretic deposition. The FT-IR, SEM, TEM, and EDX techniques have been employed for the structural, morphological, and composition analysis of the fabricated electrodes. The morphological studies clearly reveal uniform inter-linking and dispersion of hexagonal nanograins of ZrO(2) (30-50 nm) into the chitosan matrix, resulting in homogeneous nanobiocomposite formation. Electrochemical response measurements of DNA/CHIT-NanoZrO(2)/ITO bioelectrode, carried out using cyclic voltammetry and differential pulse voltammetry, reveal that this bioelectrode can specifically detect complementary target DNA up to 0.00078 μM with sensitivity of 6.38 × 10(-6) AμM(-1).
authors.affiliation: Department of Science & Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Materials Physics & Engineering Division, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
authors.forename: Maumita
authors.initials: M
authors.lastname: Das
authors.forename: Chetna
authors.initials: C
authors.lastname: Dhand
authors.forename: Gajjala
authors.initials: G
authors.lastname: Sumana
authors.forename: A K
authors.initials: AK
authors.lastname: Srivastava
authors.forename: R
authors.initials: R
authors.lastname: Nagarajan
authors.forename: Lata
authors.initials: L
authors.lastname: Nain
authors.forename: M
authors.initials: M
authors.lastname: Iwamoto
authors.forename: Takaaki
authors.initials: T
authors.lastname: Manaka
authors.forename: B D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D053761:Nanocomposites / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D009696:Nucleic Acids; D048271:Chitosan; D015040:Zirconium; C028541:zirconium oxide