title: The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives.
abstract: Using evolved pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs, L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Yane-Shih
authors.initials: YS
authors.lastname: Wang
authors.forename: William K
authors.initials: WK
authors.lastname: Russell
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wan
authors.forename: Lindsey E
authors.initials: LE
authors.lastname: Dodd
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Pai
authors.forename: David H
authors.initials: DH
authors.lastname: Russell
authors.forename: Wenshe R
authors.initials: WR
authors.lastname: Liu
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D018389:Codon, Nonsense / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics*; D015202:Protein Engineering; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D018389:Codon, Nonsense; D029968:Escherichia coli Proteins; D010649:Phenylalanine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine