title: Preparation and characterization of iminodiacetic acid-functionalized magnetic nanoparticles and its selective removal of bovine hemoglobin.
abstract: In this study, a novel route for the preparation of magnetite (Fe(3)O(4)) nanoparticles (NPs) with immobilized metal affinity ligand iminodiacetic acid (IDA) charged with Cu(2+) was developed. First, magnetite nanoparticles were synthesized by a hydrothermal method. Charged with Cu(2+), the magnetic nanoparticles (MNPs) were applied to separate a model protein mixture of bovine hemoglobin (BHb) and bovine serum albumin (BSA). They could be separated completely and showed low non-specific adsorption. The morphology, structure and composition of the magnetite MNPs were characterized by transmission electron microscopy, power x-ray diffraction, x-ray photoelectron spectrometry and Fourier transform infrared spectroscopy. The resulting magnetite MNPs charged with Cu(2+) show not only a strong magnetic response to externally applied magnetic field, but are also highly specific to protein BHb. It is interesting that MNPs modified with metal ligands showed a property of magnetic colloid photonic crystals. Furthermore, they could efficiently remove the abundant protein bovine hemoglobin from bovine blood. They have potential application in removing abundant protein in proteomic analysis.
authors.affiliation: Department of Chemistry, Nankai University, Tianjin, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiwen
authors.initials: X
authors.lastname: He
authors.forename: Langxing
authors.initials: L
authors.lastname: Chen
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000302:isolation & purification* / Q000378:metabolism; D007098:Imino Acids / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D012710:Serum Albumin, Bovine / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D006454:Hemoglobins; D007098:Imino Acids; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine; D003300:Copper; C008109:iminodiacetic acid