title: Galactose-functionalized magnetic iron-oxide nanoparticles for enrichment and detection of ricin toxin.
abstract: To generate a new specific recognition module for the enrichment and detection of ricin, galactose-functionalized silanized magnetic iron-oxide nanoparticles (Gal-SiMNPs) were assembled by amino-silanized MNPs (amino-SiMNPs) and a galactose linker. Amino-SiMNPs were produced by a coprecipitation method, and were coated with double layers of silica and aminosilane by hydrolysis of tetraethoxysilane and 3-aminopropyltriethoxysilane, respectively. A galactose with an amido-acid linker was synthesized by four steps of chemical modification from O-acetyl protected galactose. The diameters of Gal-SiMNPs were characterized as being 60 ± 20 nm. The average amount of galactose-loading and ricin-binding on Gal-SiMNPs was 30 ± 2 µg galactose and 29 ± 2 µg ricin toxin on the surface of 1 mg of Gal-SiMNPs, respectively. Furthermore, a rapid, simple and efficient colorimetric assay was established for the detection of ricin based on the Gal-SiMNPs, and the limits of detection (LODs) of 2 and 4 ng/mL for ricin in physiological buffer and serum were obtained, respectively.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, No.27 Tai Ping Road, Hai Dian District, Beijing 100850, P. R. China.
authors.forename: He-Zhu
authors.initials: HZ
authors.lastname: Liu
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Tang
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Xie
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Wang
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D005690:Galactose / Q000138:chemical synthesis / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012276:Ricin / Q000032:analysis*; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D012276:Ricin; D005690:Galactose