title: Dissimilar chromatographic systems to indicate and identify antioxidants from Mallotus species.
abstract: The genus of Mallotus contains several species commonly used as traditional medicines in oriental countries. A data set containing 39 Mallotus samples, differing in species, cultivation conditions, harvest season and/or part of the plant was used to develop fingerprints on two dissimilar chromatographic systems. An exploratory analysis with principal component analysis (PCA) was performed on both data sets individually. The results were also combined to obtain additional information on the unknown samples included in the data set. Furthermore, the antioxidant activity of the samples was measured and modelled as a function of the fingerprints using the orthogonal projections to latent structures (O-PLS) technique. The regression coefficients of the models were studied to indicate the peaks potentially responsible for the antioxidant activity. The indicated peaks were analyzed and identified by HPLC coupled to mass spectrometry (HPLC-MS). Because of the complexity of biological samples, it was aspired to separate co-eluting components based on the significant difference in chromatographic selectivity on the dissimilar systems and consequently obtain additional, complementary information on the contribution of the individual components to the antioxidant activity. The results illustrate the potential use of dissimilar chromatographic systems. Several initially co-eluting compounds could be separated on the dissimilar system. The corresponding regression coefficients provided complementary information on the potential antioxidant activity of the separated compounds.
authors.affiliation: Department of Analytical Chemistry and Pharmaceutical Technology Center for Pharmaceutical Research, Vrije Universiteit Brussel - VUB, Laarbeeklaan 103, B-1090 Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Tistaert
authors.forename: B
authors.initials: B
authors.lastname: Dejaegher
authors.forename: G
authors.initials: G
authors.lastname: Chataigné
authors.forename: C
authors.initials: C
authors.lastname: Van Minh
authors.forename: J
authors.initials: J
authors.lastname: Quetin-Leclercq
authors.forename: Y
authors.initials: Y
authors.lastname: Vander Heyden
mesh_terms: D053000:Analytic Sample Preparation Methods; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D016018:Least-Squares Analysis; D036461:Mallotus Plant / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D025341:Principal Component Analysis
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl