title: [Study on serum differentially expressed proteins in discordance of congenital esotropic phenotypes in monozygotic twins].
abstract: OBJECTIVE
  To investigate the serum differentially expressed proteins in discordance of congenital esotropia phenotypes in monozygotic twins and non-strabismus ones.
  
  
  METHODS
  SELDI-TOF-MS technology was used to detect the changes of protein expression in a couple of twins with phenotype discordance esotropia (twins A is orthotropia and twins B is esotropia). In addition, two non-twins esotropia and 2 orthotropia children with the same age were chosen. The obtained serum differential expression proteome was validated in 12 non-twins esotropia and 18 orthotropia children and initial predication by proteinum database.
  
  
  RESULTS
  The result of SELDI-TOF-MS in 6 serums showed that four differential expression protein peaks, the molecular weight of these different proteins were 4146, 4801, 7786 and 5859, and the former 3 proteins were down-regulated and last one was up-regulated. Their features were fairly coincident with glucagons precursor, pituitary adenylate cyclase activating peptide (PACAP), cAMP dependent protein kinase inhibitor α and anti-metastasis gene (antigen).
  
  
  CONCLUSIONS
  The expression of different proteins among the esotropic and orthotopic children was existed. The expression of different proteins was main down-regulated in the strabismus patients. Glucagons precursor, protein kinase A inhibitor α and PACAP may be related with occurrence of congenital esotropia.
authors.affiliation: The Department of Ophthalmolog, the Affiliated Hospital of Qingdao University, Medical College, Qingdao 266003, China. liugx7@126.com
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: Liu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D002675:Child, Preschool; D004200:Diseases in Twins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D004948:Esotropia / Q000097:blood* / Q000151:congenital / Q000235:genetics; D006801:Humans; D008297:Male; D010641:Phenotype; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D014430:Twins, Monozygotic
chemical_list: D020543:Proteome