title: Chlamydia antibody testing and diagnosing tubal pathology in subfertile women: an individual patient data meta-analysis.
abstract: BACKGROUND
  The Chlamydia IgG antibody test (CAT) shows considerable variations in reported estimates of test accuracy, partly because of the use of different assays and cut-off values. The aim of this study was to reassess the accuracy of CAT in diagnosing tubal pathology by individual patient data (IPD) meta-analysis for three different CAT assays.
  
  
  METHODS
  We approached authors of primary studies that used micro-immunofluorescence tests (MIF), immunofluorescence tests (IF) or enzyme-linked immunosorbent assay tests (ELISA). Using the obtained IPD, we performed pooled receiver operator characteristics analysis and logistic regression analysis with a random effects model to compare the three assays. Tubal pathology was defined as either any tubal obstruction or bilateral tubal obstruction.
  
  
  RESULTS
  We acquired data of 14 primary studies containing data of 6191 women, of which data of 3453 women were available for analysis. The areas under the curve for ELISA, IF and MIF were 0.64, 0.65 and 0.75, respectively (P-value < 0.001) for any tubal pathology and 0.66, 0.66 and 0.77, respectively (P-value = 0.01) for bilateral tubal pathology.
  
  
  CONCLUSIONS
  In Chlamydia antibody testing, MIF is superior in the assessment of tubal pathology. In the initial screen for tubal pathology MIF should therefore be the test of first choice.
authors.affiliation: Centre for Reproductive Medicine, Department of Obstetrics and Gynaecology, Academic Medical Centre (AMC), Amsterdam, The Netherlands. k.a.broeze@amc.uva.nl
authors.forename: K A
authors.initials: KA
authors.lastname: Broeze
authors.forename: B C
authors.initials: BC
authors.lastname: Opmeer
authors.forename: S F P J
authors.initials: SF
authors.lastname: Coppus
authors.forename: N
authors.initials: N
authors.lastname: Van Geloven
authors.forename: M F C
authors.initials: MF
authors.lastname: Alves
authors.forename: G
authors.initials: G
authors.lastname: Anestad
authors.forename: S
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: J
authors.initials: J
authors.lastname: Allan
authors.forename: M F
authors.initials: MF
authors.lastname: Guerra-Infante
authors.forename: J E
authors.initials: JE
authors.lastname: Den Hartog
authors.forename: J A
authors.initials: JA
authors.lastname: Land
authors.forename: A
authors.initials: A
authors.lastname: Idahl
authors.forename: P J Q
authors.initials: PJ
authors.lastname: Van der Linden
authors.forename: J W
authors.initials: JW
authors.lastname: Mouton
authors.forename: E H Y
authors.initials: EH
authors.lastname: Ng
authors.forename: J W
authors.initials: JW
authors.lastname: Van der Steeg
authors.forename: P
authors.initials: P
authors.lastname: Steures
authors.forename: H F
authors.initials: HF
authors.lastname: Svenstrup
authors.forename: A
authors.initials: A
authors.lastname: Tiitinen
authors.forename: B
authors.initials: B
authors.lastname: Toye
authors.forename: F
authors.initials: F
authors.lastname: Van der Veen
authors.forename: B W
authors.initials: BW
authors.lastname: Mol
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005184:Fallopian Tube Diseases / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012107:Research Design; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G