title: Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus.
abstract: Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. This process, conserved among dsDNA viruses such as herpes viruses and bacteriophages, is key to forming infectious virions. Bacteriophage P22 has served as a model system for this study in the past several decades. However, how capsid assembly is initiated, where and how scaffolding proteins bind to coat proteins in the procapsid, and the conformational changes upon capsid maturation still remain elusive. Here, we report Cα backbone models for the P22 procapsid and infectious virion derived from electron cryomicroscopy density maps determined at 3.8- and 4.0-Å resolution, respectively, and the first procapsid structure at subnanometer resolution without imposing symmetry. The procapsid structures show the scaffolding protein interacting electrostatically with the N terminus (N arm) of the coat protein through its C-terminal helix-loop-helix motif, as well as unexpected interactions between 10 scaffolding proteins and the 12-fold portal located at a unique vertex. These suggest a critical role for the scaffolding proteins both in initiating the capsid assembly at the portal vertex and propagating its growth on a T = 7 icosahedral lattice. Comparison of the procapsid and the virion backbone models reveals coordinated and complex conformational changes. These structural observations allow us to propose a more detailed molecular mechanism for the scaffolding-mediated capsid assembly initiation including portal incorporation, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.
authors.affiliation: National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Chen
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Baker
authors.forename: Corey F
authors.initials: CF
authors.lastname: Hryc
authors.forename: Frank
authors.initials: F
authors.lastname: DiMaio
authors.forename: Joanita
authors.initials: J
authors.lastname: Jakana
authors.forename: Weimin
authors.initials: W
authors.lastname: Wu
authors.forename: Matthew
authors.initials: M
authors.lastname: Dougherty
authors.forename: Cameron
authors.initials: C
authors.lastname: Haase-Pettingell
authors.forename: Michael F
authors.initials: MF
authors.lastname: Schmid
authors.forename: Wen
authors.initials: W
authors.lastname: Jiang
authors.forename: David
authors.initials: D
authors.lastname: Baker
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: King
authors.forename: Wah
authors.initials: W
authors.lastname: Chiu
mesh_terms: D017100:Bacteriophage P22 / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D002213:Capsid / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism; D020285:Cryoelectron Microscopy; D004267:DNA Viruses / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins; C074954:VP3 protein, Bluetongue virus; D014758:Viral Core Proteins; D014764:Viral Proteins