title: New original in vitro method to assess cholinesterase reactivity in organophosphate poisoning.
abstract: Assessment of organophosphate poisoning could benefit from a safe, non-expensive, easy to perform, quick (< 1 hour) test, which evaluates the level of cholinesterase activity "in vitro" regarding to the capability of oximes to reactivate OF-blocked cholinesterase. In the proposed protocol, 0.5 mL of sample serum is incubated, prior to the evaluation of level of cholinesterase activity, with 5 microL of a Toxogonin dilution (0.125 mg) for 30 minutes at 37 degrees C. For the standardization of the newly proposed protocol, several important issues were documented in the present article. The new original method of assessing cholinesterase reactivability will consist in an advantage for the diagnosis, prognostic evaluation and therapeutic orientation in OF intoxication.
authors.affiliation: Internal Medicine Department, School of Medicine, Gr.T. Popa University of Medicine and Pharmacy Iaşi.
authors.forename: L
authors.initials: L
authors.lastname: Sorodoc
authors.forename: Cătălina
authors.initials: C
authors.lastname: Lionte
authors.forename: Ema
authors.initials: E
authors.lastname: Largu
authors.forename: O
authors.initials: O
authors.lastname: Petriş
mesh_terms: D002801:Cholinesterase Reactivators / Q000494:pharmacology* / Q000627:therapeutic use; D002802:Cholinesterases / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D009768:Obidoxime Chloride / Q000494:pharmacology* / Q000627:therapeutic use; D062025:Organophosphate Poisoning; D011041:Poisoning / Q000201:enzymology
chemical_list: D002801:Cholinesterase Reactivators; D009768:Obidoxime Chloride; D002802:Cholinesterases