title: [Autophagy of prostate cancer PC-3 cells under the induction of oridonin].
abstract: OBJECTIVE
  To investigate the mechanism of oridonin (ORI)-induced autophagy in prostate cancer PC-3 cells.
  
  
  METHODS
  The PC-3 cells cultured in vitro were treated with ORI. The inhibitory ratio of oridonin In PC-3 cells was assayed by MTT. The ultrastructural cellular changes were observed under light microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM). AO staining was used to observe the acidic vesicular organelles (AVOs). The level of MAP1-LC3 was detected by Western blot and reverse transcriptase polymerase chain reaction (RT-PCR) used to detect the level of mRNA of beclin 1.
  
  
  RESULTS
  After oridonin treatment, the proliferation of PC-3 cells was inhibited significantly in a concentration and time-dependent manner. The SEM examination revealed cellular shrinkage and the disappearance of surface microvilli after ORI treatment. And on the TEM examination, the nuclei exhibited chromatin condensation and the appearance of a large number of autophagosome with double-membrane structure in cytoplasm. AO staining showed the existence of AVOs. Simultaneously, the expressions of autophagy-related proteins, MAP-LC3 and the mRNA level of beclin 1 were elevated by ORI. The autophagy inhibitor 3-MA reversed the elevation of beclin 1 mRNA.
  
  
  CONCLUSIONS
  The growth of PC-3 cells is inhibited. And cellular is induced by oridonin with a potential anti-tumor effect.
authors.affiliation: Shaoxin Central Hospital, Shaoxin 312030, China. LLH7966@126.com
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Ye
authors.forename: Yong-liang
authors.initials: YL
authors.lastname: Chen
authors.forename: Shui-xiang
authors.initials: SX
authors.lastname: Tao
authors.forename: Xiao-qiang
authors.initials: XQ
authors.lastname: Jiang
authors.forename: Wang-jian
authors.initials: WJ
authors.lastname: Li
authors.forename: Wei-liang
authors.initials: WL
authors.lastname: Qian
authors.forename: Jian-song
authors.initials: JS
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045786:Diterpenes, Kaurane / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology
chemical_list: D045786:Diterpenes, Kaurane; C011959:oridonin