title: Proteomic analysis of male 4C germ cell proteins involved in mouse meiosis.
abstract: Male meiosis is a specialized type of cell division that gives rise to sperm. Errors in this process can result in the generation of aneuploid gametes, which are associated with birth defects and infertility in humans. Until now, there has been a lack of a large-scale identification of proteins involved in male meiosis in mammals. In this study, we report the high-confidence identification of 3625 proteins in mouse male germ cells with 4C DNA content undergoing meiosis I. Of these, 397 were found to be testis specific. Bioinformatics analysis of the proteome led to the identification of 28 proteins known to be essential for male meiosis in mice. We also found 172 proteins that had yeast orthologs known to be essential for meiosis. Chromosome distribution analysis of the proteome showed underrepresentation of the identified proteins on the X chromosome, which may be due to meiotic sex chromosome inactivation. Characterization of the proteome of 4C germ cells from mouse testis provides an inventory of proteins, which is useful for understanding meiosis and the mechanisms of male infertility.
authors.affiliation: Department of Histology and Embryology, Nanjing Medical University, Nanjing, P R China.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Qi
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Chen
authors.forename: Zuomin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
mesh_terms: D000818:Animals; D005455:Fluorescent Antibody Technique; D008297:Male; D008540:Meiosis; D051379:Mice; D008813:Mice, Inbred ICR; D010455:Peptides / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014960:X Chromosome / Q000378:metabolism
chemical_list: D010455:Peptides; D020543:Proteome