title: Bioorthogonal chemical tagging of protein cholesterylation in living cells.
abstract: We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.
authors.affiliation: Department of Chemistry, Imperial College London, Exhibition Rd., London SW7 2AZ, UK.
authors.forename: William P
authors.initials: WP
authors.lastname: Heal
authors.forename: Biljana
authors.initials: B
authors.lastname: Jovanovic
authors.forename: Sara
authors.initials: S
authors.lastname: Bessin
authors.forename: Megan H
authors.initials: MH
authors.lastname: Wright
authors.forename: Anthony I
authors.initials: AI
authors.lastname: Magee
authors.forename: Edward W
authors.initials: EW
authors.lastname: Tate
mesh_terms: D000345:Affinity Labels / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002460:Cell Line; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D019793:Fluorescein / Q000737:chemistry; D053823:Hedgehog Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D054878:Lipoylation; D011499:Protein Processing, Post-Translational; D012235:Rhodamines / Q000737:chemistry
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D000345:Affinity Labels; D001386:Azides; D053823:Hedgehog Proteins; D012235:Rhodamines; D002784:Cholesterol; D019793:Fluorescein