title: Pharmacokinetics of oxytetracycline in hemolymph from the Chinese mitten crab, Eriocheir sinensis.
abstract: The purpose of this study was to investigate the hemolymph kinetics and depuration time of oxytetracycline following intramuscular administration at doses of 2, 8 and 40 mg/kg body weight, respectively. The concentration of OTC in hemolymph was assayed using solid phase extraction and high performance liquid chromatography. The elimination half-life of the terminal part of the elimination phase (t(1/2β) ) ranged from 87.9 to 114.3 h. The total body clearance (CL(b) ) was 0.0430 L/kg/h at the lower dose, 0.0123 L/kg/h at the medium dose and 0.0013 L/kg/h at the higher dose. The apparent volume of the central compartment (V(c) ) was found to be 1.383, 0.699 and 0.143 L/kg respectively. The depuration time for each dose was 13.6, 29.6 and 57.6 days, respectively. Results from the present study suggest that the 40 mg/kg dose might have the best therapeutic efficacy following intramuscular administration.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory of Aquatic Crustacean Animal Diseases, College of Life Sciences, Nanjing Normal University, Nanjing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Feng
authors.forename: G H
authors.initials: GH
authors.lastname: Wu
authors.forename: T M
authors.initials: TM
authors.lastname: Liang
authors.forename: H Y
authors.initials: HY
authors.lastname: Ji
authors.forename: X J
authors.initials: XJ
authors.lastname: Jiang
authors.forename: W
authors.initials: W
authors.lastname: Gu
authors.forename: W
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D019540:Area Under Curve; D003386:Brachyura / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006207:Half-Life; D006458:Hemolymph / Q000737:chemistry*; D010118:Oxytetracycline / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D000900:Anti-Bacterial Agents; D010118:Oxytetracycline