title: Binding specificity of the recombinant cytoplasmic domain of Cordyceps militaris β-1,3-glucan synthase catalytic subunit.
abstract: The cytoplasmic domain of the medicinal mushroom Cordyceps militaris β-1,3-glucan synthase catalytic subunit Fks1 was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and was assayed for binding specificity. The recombinant cytoplasmic domain bound specifically to UDP-agarose and lichenan (β-glucan), but not to ADP-agarose, GDP-agarose, or other carbohydrates.
authors.affiliation: Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan. ujita@meijo-u.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Ujita
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Inoue
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Makino
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Katsuno
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okumura
mesh_terms: D020134:Catalytic Domain; D032661:Cordyceps / Q000166:cytology* / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology*; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity; D014530:Uridine Diphosphate / Q000378:metabolism; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D047071:beta-Glucans; D014530:Uridine Diphosphate; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase