title: Molecularly imprinted solid-phase extraction for the selective determination of valnemulin in feeds with high performance liquid chromatography.
abstract: A simple, sensitive and reproducible high performance liquid chromatographic method was developed for determining valnemulin in feeds. Feed samples were extracted with ethyl acetate under alkaline condition, cleaned up by molecularly imprinted solid-phase extraction, and analyzed by high performance liquid chromatography with ultraviolet detection. The characteristics of the synthesized polymer were evaluated and the loading capacity of the polymer was about 1000 μg analyte/g imprinted polymer. The new procedure for the feed sample cleanup using the prepared polymer cartridge gave higher recoveries and fewer matrix interferences. The assay exhibited a linear dynamic range of 5.0-200 mg kg(-1) with the correlation coefficient above 0.9993. Recoveries of valnemulin from feed samples spiked at 5.0, 20 and 50 mg kg(-1) ranged between 76.0% and 94.4% with relative standard deviations of less than 9%. The limit of detection for valnemulin in feeds was 1 mg kg(-1).
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Guo
authors.forename: Kaiyong
authors.initials: K
authors.lastname: Liu
authors.forename: Yahong
authors.initials: Y
authors.lastname: Liu
authors.forename: Binghu
authors.initials: B
authors.lastname: Fang
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Limin
authors.initials: L
authors.lastname: He
authors.forename: Zhenling
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D000085:Acetates; D000641:Ammonia; D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis / Q000737:chemistry; D016014:Linear Models; D000432:Methanol; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000085:Acetates; D004224:Diterpenes; C117845:valnemulin; D000641:Ammonia; C007650:ethyl acetate; D000432:Methanol