title: Development and validation of an analytical method for determination of endocrine disruptor, 2,4-D, in paddy field water.
abstract: The acidic herbicides are an important class of chemical compounds that are used to control a variety of weeds that threaten many crops. Owing to their low microbial activity levels, the acidic herbicides exhibit a residual activity remaining for periods of up to several months in soils and water. The principal objective of this study was to develop an analytical method based on liquid-liquid and solid-phase extraction followed by HPLC, for the determination of 2,4-D in paddy field water. The residues were verified via tandem mass spectrometry (MS/MS) in negative-ion electrospray ionization (ESI) mode. Linearity was good over a concentration range of 1-100 µg/L with a correlation coefficient (r(2) ) of 0.999. The mean recovery rates of triplicate results ranged from 85.2 to 90.85%. The limits of detection and quantitation were 0.4 and 1.0 µg/L, respectively. The method proposed herein was applied to field samples acquired from Hampyung and Sunchang counties, Republic of Korea. The analyte was detected at a concentration range of 6.8-12.8 and 3.55-24.0 µg/L, respectively.
authors.affiliation: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Jeong-Heui
authors.initials: JH
authors.lastname: Choi
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Kim
authors.forename: Jong-Hyouk
authors.initials: JH
authors.lastname: Park
authors.forename: Woo Jung
authors.initials: WJ
authors.lastname: Choi
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D052244:Endocrine Disruptors; D006540:Herbicides; D014874:Water Pollutants, Chemical; D015084:2,4-Dichlorophenoxyacetic Acid