title: Plexin-B1 is a target of miR-214 in cervical cancer and promotes the growth and invasion of HeLa cells.
abstract: Plexin-B1, the receptor for Sema4D, has been reported to trigger multiple and sometimes opposing cellular responses in various types of tumor cells. It has been implicated in the regulation of tumor-cell survival, proliferation, angiogenesis, invasion and metastasis. However, the plexin-B1 gene expression and its regulatory mechanism in cervical cancer remain unclear. The present study shows that plexin-B1 is over-expressed in cervical tumor tissues compared to normal cervical tissues by immunohistochemistry, Western blotting and quantitative RT-PCR. The expression of plexin-B1 is significantly associated with cervical tumor metastasis and invasion according to the analysis of the clinicopathologic data. Plexin-B1 also promotes proliferation, migration and invasion in human cervical cancer HeLa cells. We also found that the plexin-B1 levels are inversely correlated with miR-214 amounts in both cervical cancer tissues and HeLa cells. And miR-214 expression level is also associated with metastasis and invasion of cervical tumor. Furthermore, we demonstrate that plexin-B1 is inhibited by miR-214 through a miR-214 binding site within the 3'UTR of plexin-B1 in HeLa cells. Ectopic expression of miR-214 could inhibit the proliferation capacity, migration and invasion ability of HeLa cells. Our findings suggest that plexin-B1, a target of miR-214, may function as an oncogene in human cervical cancer HeLa cells.
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Qiang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Chen
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Wan
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Song-Yuan
authors.initials: SY
authors.lastname: Gao
authors.forename: Bao-Cun
authors.initials: BC
authors.lastname: Sun
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D001483:Base Sequence; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; C492119:PLXNB1 protein, human; D011956:Receptors, Cell Surface