title: Preserved and unpreserved 12 anti-allergic ophthalmic solutions and ocular surface toxicity: in vitro assessment in four cultured corneal and conjunctival epithelial cell lines.
abstract: In the present study, we evaluated the cytotoxicity of anti-allergic ophthalmic solutions in cultured corneal and conjunctival cells, namely SIRC (rabbit corneal epithelium), BCE C/D-1b (bovine corneal epithelial cells), RC-1 (rabbit corneal epithelium), and Chang (human conjunctival cells). The viability of cell cultures was determined following the exposure of cells to 12 commercially available anti-allergic ophthalmic solutions for varying exposure times and at various dilutions using the MTT and neutral red assays. The cell viability score (CVS) was used to compare the toxicity of different drugs. Based on CVS data, the order of cell viability after exposure to the drugs was Zepelin ≥ Tramelas PF ≥ Cumorol PF ≥ Ketotifen PF ≥ Eyevinal = Fumarton ≥ Cumorol > Intal ≥ Rizaben ≥ Tramelas ≥ Patanol Livostin. In conclusion, cell viability was mostly affected by the concentration of benzalkonium chloride rather than the active component and/or the anti-allergic action of the drug. The CVS was useful in comparing the toxicity of different drugs.
authors.affiliation: Department of Ophthalmology, Saitama National Hospital. mayaki@olive.ocn.ne.jp
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ayaki
authors.forename: Atsuo
authors.initials: A
authors.lastname: Iwasawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yaguchi
authors.forename: Ryohei
authors.initials: R
authors.lastname: Koide
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000633:toxicity*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003228:Conjunctiva / Q000166:cytology / Q000187:drug effects*; D003315:Cornea / Q000166:cytology / Q000187:drug effects*; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D009883:Ophthalmic Solutions / Q000633:toxicity*; D011310:Preservatives, Pharmaceutical; D011817:Rabbits; D018570:Risk Assessment
chemical_list: D018926:Anti-Allergic Agents; D009883:Ophthalmic Solutions; D011310:Preservatives, Pharmaceutical