title: [Association of interleukin-10 gene polymorphism with cachexia in patients with gastric cancer].
abstract: OBJECTIVE
  To investigate whether the single nucleotide polymorphisms (SNPs) at -1082, -819 and -592 of interleukin-10 gene and its haplotype are associated with cachexia in patients with gastric cancer.
  
  
  METHODS
  Radioimmunoassay was used to examine the serum levels of IL-10 in 223 patients with gastric cancer. The single nucleotide polymorphisms (SNPs) of IL-10 gene -1082G/A, -819T/C and -592A/C were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The serum levels of IL-10 were significantly higher in patients with cachexia than those without (P < 0.001). An increased frequency of -1082G allele was noted in patients with cachexia (P = 0.049). The frequencies of -1082AG and -819CC genotypes were elevated in patients with cachexia than those without (P = 0.036, 0.024). In a logistic regression analysis adjusted for actual weight, carcinoma location and stage, the -1082AG genotype was associated with an odds ratio of 1.989 (95%CI, 1.041 - 3.802, P = 0.037), and the -819CC genotype with an odds ratio of 3.393 (95%CI, 1.298 - 8.871, P = 0.013) for cachexia. Furthermore, haplotype analysis revealed that G1082C819C592 haplotype was associated with a significantly increased risk of cachexia (OR = 2.21; 95%CI, 1.14 - 4.30; P = 0.02).
  
  
  CONCLUSIONS
  Our results suggest that the gene haplotype of IL-10 contributes to the occurrence of cachexia in patients with gastric cancer in Chinese population.
authors.affiliation: Department of General Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China.
authors.forename: Feng-bo
authors.initials: FB
authors.lastname: Sun
authors.forename: Dian-liang
authors.initials: DL
authors.lastname: Zhang
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Zheng
authors.forename: Bo
authors.initials: B
authors.lastname: Song
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D002100:Cachexia / Q000097:blood / Q000150:complications / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000097:blood / Q000150:complications / Q000235:genetics*
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10