title: COX1 and COX2 expression in non-neuronal cellular compartments of the rhesus macaque brain during lentiviral infection.
abstract: Recent evidence suggests that cyclooxygenases COX1 and COX2 differentially affect brain immunity. Limited data exist about their expressional changes in neurodegenerative diseases such as neuro-AIDS. Here, we analyzed the regulation of non-neuronal COX1/2 expression in rhesus macaque brain during infection with SIV(δ670) and antiretroviral treatment. COX1 was constitutively expressed in microglia and endothelial cells and was not changed in early SIV infection. Late stage of disease was characterized by increased COX1 expression in globally activated microglia, macrophage nodules, infiltrates, and multinucleated giant cells. Endothelial COX1 expression was unaltered. In contrast, COX2 was not expressed in non-neuronal cells in the brain of uninfected and asymptomatically SIV-infected monkeys but was induced in nodule- and syncytium-forming macrophages and in endothelial cells in areas with infiltrates and SIV in monkeys with AIDS. Antiretroviral treatment of AIDS-diseased monkeys with 6-chloro-2',3'-dideoxyguanosine markedly reduced SIV burden, appearance of COX1-positive macrophage nodules, giant cells, and infiltrates, and COX2 induction in the brain. However, the number of COX1-positive diffuse microglia was still increased in antiretrovirally treated animals as compared to uninfected or asymptomatic SIV-infected monkeys. Our data imply that both COX isoforms are differentially regulated and may distinctly modulate local immune responses in the brain during lentiviral disease.
authors.affiliation: Molecular Neuroscience, Department of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
authors.forename: Candan
authors.initials: C
authors.lastname: Depboylu
authors.forename: Eberhard
authors.initials: E
authors.lastname: Weihe
authors.forename: Lee E
authors.initials: LE
authors.lastname: Eiden
mesh_terms: D000818:Animals; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D001921:Brain / Q000201:enzymology* / Q000473:pathology / Q000821:virology*; D051545:Cyclooxygenase 1 / Q000096:biosynthesis*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D015224:Dideoxynucleosides / Q000627:therapeutic use; D004195:Disease Models, Animal; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D015302:Simian Immunodeficiency Virus
chemical_list: C095793:6-chloro-2',3'-dideoxyguanosine; D044966:Anti-Retroviral Agents; D015224:Dideoxynucleosides; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2