title: The cytotoxic effects of some selected gold(III) complexes on 4T1 cells and their role in the prevention of breast tumor growth in BALB/c mice.
abstract: OBJECTIVE
  to investigate the cytotoxic activity of newly synthesized gold(III) complexes [AuCl(2)(en)](+), [AuCl(2) (SMC)](+), [AuCl(2)(DMSO)(2)(+) (en: ethylenediamine, SMC: S-methyl- L-cysteine and DMSO: for dimethylsulfoxide) in 4T1 mouse breast cancer cell line in vitro and in vivo and to compare their antitumor characteristics with cisplatin complex [PtCl(2)(NH(3))(2)].
  
  
  METHODS
  the in vitro, effects of the tested complexes on 4T1 cell viability were determined using MTT colorimetric technique. In vivo, progression of mouse breast tumor growth in BALB/c mice was measured by using external caliper.
  
  
  RESULTS
  among the tested gold(III) complexes, [AuCl(2) (en)](+) showed best cytotoxic effects in vitro. The cytotoxic effects of [AuCl(2)(en)](+) and [PtCl(2)(NH(3))(2)] were similar at all concentrations. The data from the in vivo experiment showed that among the tested gold(III) complexes only [AuCl(2)(en)](+) can prevent the primary breast tumor growth. [AuCl(2)(en)](+) was tolerated well and much better than [AuCl(2)(DMSO)(2)(+), [AuCl(2)(SMC)](+) and [PtCl(2)(NH(3))(2)] complex which was confirmed by weight gain in mice that received [AuCl(2)(en)](+). In addition, mice that received [AuCl(2)(en)](+) showed better survival time in comparison with mice that received [PtCl(2) (NH(3))(2)] complex.
  
  
  CONCLUSIONS
  [AuCl(2) (en)](+) complex seems to be good candidate for future pharmacological evaluation in breast cancer research.
authors.affiliation: Department of Microbiology and Immunology, Centre for Molecular Medicine, Faculty of Medicine, University of Kragujevac, Kragujevac, Serbia. drvolarevic@yahoo.com
authors.forename: V
authors.initials: V
authors.lastname: Volarevic
authors.forename: M
authors.initials: M
authors.lastname: Milovanovic
authors.forename: A
authors.initials: A
authors.lastname: Djekovic
authors.forename: B
authors.initials: B
authors.lastname: Petrovic
authors.forename: N
authors.initials: N
authors.lastname: Arsenijevic
authors.forename: Z D
authors.initials: ZD
authors.lastname: Bugarcic
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D050607:Organogold Compounds / Q000138:chemical synthesis / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D050607:Organogold Compounds