title: Purification, crystallization and X-ray diffraction study of basic 7S globulin from soybean.
abstract: Basic 7S globulin (Bg7S) is expressed by soybeans in response to biotic or abiotic stress. Bg7S is capable of binding to a 4 kDa protein which is supposedly involved in cell proliferation. Bg7S is widely found not only in legumes, but also in other plants; however, its function is still unclear. Here, Bg7S was successfully crystallized. Orthorhombic and monoclinic crystals of Bg7S were obtained under different conditions and belonged to space groups P2(1)2(1)2, with unit-cell parameters a=111.9, b=130.1, c=287.8 Å, and P2(1), with unit-cell parameters a=85.3, b=137.6, c=162.1 Å, β=91.2°, respectively.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshizawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Mayuki
authors.initials: M
authors.lastname: Yamabe
authors.forename: Naoki
authors.initials: N
authors.lastname: Shichijo
authors.forename: Kazuki
authors.initials: K
authors.lastname: Hanada
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hirano
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
mesh_terms: D052179:Antigens, Plant / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005916:Globulins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D030262:Soybean Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max