title: Evaluation of a novel rapid detection system for yeasts and molds.
abstract: A novel system, the NISSUI rapid detection system, has been developed to rapidly detect yeasts and molds in food. This system consists of a liquid medium containing resazurin as a redox indicator, a unique original micro-well dish containing 676 micro-wells, and a fluorescence-monitoring instrument with an incubator. To evaluate this system, orange juice, milk, and physiological saline solutions artificially inoculated with yeasts or molds were used as samples. Comparison of the new system used at 28ºC for 48 h with a spread-plate method using a potato-dextrose-agar plate at 25ºC for 120 h yielded a correlation coefficient (r) of 0.95. Our data reveal that the new method considerably shortens the time required for detection of yeasts and molds in food.
authors.affiliation: Research Department, Nissui Pharmaceutical Co. LTD., Ibaraki, Japan. k-tominaga@yki.nissui-pharm.jp
authors.forename: Kei
authors.initials: K
authors.lastname: Tominaga
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ii
authors.forename: Fumi
authors.initials: F
authors.lastname: Kashiwabara
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Hara
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Uesaka
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kodaka
mesh_terms: D003470:Culture Media; D005516:Food Microbiology; D005658:Fungi / Q000302:isolation & purification*; D008828:Microbiological Techniques; D015003:Yeasts / Q000302:isolation & purification*
chemical_list: D003470:Culture Media