title: Understanding paternal genome demethylation through live-cell imaging and siRNA.
abstract: Identification of a DNA demethylase responsible for zygotic paternal DNA demethylation has been one of the most challenging goals in the field of epigenetics. Several candidate molecules have been proposed, but their involvement in the demethylation remains controversial, partly due to the difficulty of preparing a sufficient quantity of materials for biochemical analysis. In this review, we utilize a recently developed method for live-cell imaging of mouse zygotes combined with RNA interference (RNAi) to search for factors that affect zygotic paternal DNA demethylation. The combined use of various fluorescent probes and RNAi is a useful approach for the study of not only DNA demethylation but also the spatiotemporal dynamics of histone depositions in zygotes, although it is not appropriate for large-scale screening or knockdown of genes that are abundantly expressed before fertilization. This new technique enables us to understand the epigenetic hierarchy during cellular response and differentiation in preimplantation embryos.
authors.affiliation: Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yamagata
authors.forename: Yuki
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000818:Animals; D019175:DNA Methylation; D016678:Genome / Q000235:genetics*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D015053:Zygote / Q000378:metabolism
chemical_list: D006657:Histones; D015335:Molecular Probes; D034741:RNA, Small Interfering; D010090:Oxidoreductases, O-Demethylating