title: Conditional depletion of nuclear proteins by the Anchor Away system.
abstract: Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Fan
authors.forename: Joseph V
authors.initials: JV
authors.lastname: Geisberg
authors.forename: Koon Ho
authors.initials: KH
authors.lastname: Wong
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005818:Genetic Engineering; D005821:Genetic Techniques; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D020123:Sirolimus