title: Evaluation of osteoinduction properties of the demineralized bovine foetal growth plate powder as a new xenogenic biomaterial in rat.
abstract: The aim of this study was evaluation of osteoinductive properties of demineralized bovine foetal growth plate in submuscular transplantation (ectopic osteoinduction) as a new xenogenic biomaterial in rat model. Demineralized bovine foetal growth plate was ectopically implanted in 18 male Sprague-Dawley rats. In 18 of the animals under aseptic conditions two submuscular pouches were created between external and internal oblique abdominal muscles in the two flanks: the right was left empty (sham) and the left was filled with 20mg of demineralized bovine foetal growth plate powder. Radiographs were taken in 2, 4 and 6 weeks after the surgery, then six animals were pharmacologically euthanized after 2, 4 and 6 weeks for histopathological evaluation. Results showed: (1) osteoinductivity of xenogenic demineralized bovine foetal growth plate powder, and (2) earlier mineralization of ectopically implanted demineralized bovine foetal growth plate in the submuscular implanted area. Our results show that submuscular implantation of xenogenic demineralized bovine foetal growth plate has osteoinductive properties in a rat model.
authors.affiliation: Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. dr.bigham@gmail.com
authors.forename: A S
authors.initials: AS
authors.lastname: Bigham
authors.forename: M
authors.initials: M
authors.lastname: Shadkhast
authors.forename: A
authors.initials: A
authors.lastname: Bigham Sadegh
authors.forename: Z
authors.initials: Z
authors.lastname: Shafiei
authors.forename: A
authors.initials: A
authors.lastname: Lakzian
authors.forename: M R
authors.initials: MR
authors.lastname: Khalegi
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000000981:diagnostic imaging / Q000473:pathology / Q000637:transplantation*; D019485:Bone Morphogenetic Proteins / Q000032:analysis*; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000000981:diagnostic imaging / Q000473:pathology; D002417:Cattle; D004195:Disease Models, Animal; D006132:Growth Plate / Q000000981:diagnostic imaging / Q000473:pathology / Q000637:transplantation*; D020007:Implants, Experimental; D008297:Male; D010012:Osteogenesis; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous
chemical_list: D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes