title: Expression profile of the α subunit of the heterotrimeric G protein in rice.
abstract: Previous studies on the activity of the rice Gα promoter using a β-Glucuronidase (GUS) reporter construct indicated that Gα expression was highest in developing organs and changed in a developmental stage-dependent manner. In this paper, GUS activity derived from the rice Gα promoter was analyzed in seeds and developing leaves. In seeds, GUS activity was detected in the aleurone layer, embryo, endosperm and scutellar epithelium. In developing leaves, the activity was detected in the mesophyll tissues, phloem and xylem of the leaf sheath and in the mesophyll tissue of the leaf blade. The activity in the aleurone layer and scutellar epithelium suggests that the Gα subunit may be involved in gibberellin signaling. The activity in the mesophyll tissues of the leaf blade suggests that the Gα subunit may be related to the intensity of disease resistance. The pattern of the activity in the developing leaf also indicates that the expression of Gα follows a developmental profile at the tissue level.
authors.affiliation: Department of Bioscience, Fukui Prefectural University; Eiheiji-cho, Yoshida-gun, Fukui, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Izawa
authors.forename: Miho
authors.initials: M
authors.lastname: Minami
authors.forename: Shizuka
authors.initials: S
authors.lastname: Ohki
authors.forename: Yukimoto
authors.initials: Y
authors.lastname: Iwasaki