title: Identification and expression localization of a Ran homologue in mollusc abalone, Haliotis diversicolor supertexta.
abstract: Ran protein is a central molecular in several key nuclear functions, including nucleocytoplasmic transport, cell-cycle progression and nuclear envelope assembly. In this study, we have isolated and characterized a Ran homologue from a gastropod abalone which we named ab-Ran. The full-length cDNA consists of 1239 bp with an ORF encoding a 220 amino acid protein. The deduced amino acid sequence of ab-Ran shows highly similar to that of other Ran members (84-88%). Moreover, the ab-Ran contains five conserved regions and four carboxy-terminal residues CAAX-box. RT-PCR analysis showed that the ab-Ran was ubiquitously expressed in abalone tissues. The intracellular localization examined by immunofluorescence and immunohistochemistry staining displayed that ab-Ran was largely concentrated in the nuclei and partially in the cytoplasm. To the best of our knowledge, this is the first identification and characterization of a Ran homologue in mollusk.
authors.affiliation: College of Agronomy, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, PR China. wlj200120@163.com
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Wu
authors.forename: Mengfang
authors.initials: M
authors.lastname: Du
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049851:Gastropoda / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020931:ran GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D020931:ran GTP-Binding Protein