title: Synthesis, physicochemical and spectroscopic characterization of copper(II)-polysaccharide pullulan complexes by UV-vis, ATR-FTIR, and EPR.
abstract: Bioactive copper(II) complexes with polysaccharides, like pullulan and dextran, are important in both veterinary and human medicine for the treatment of hypochromic microcitary anemia and hypocupremia. In aqueous alkaline solutions, Cu(II) ion forms complexes with the exopolysaccharide pullulan and its reduced low-molecular derivative. The metal content and the solution composition depend on pH, temperature, and time of the reaction. The complexing process begins in a weak alkali solution (pH >7) and involves OH groups of pullulan monomer (glucopyranose) units. Complexes of Cu(II) ion with reduced low-molecular pullulan (RLMP, M(w) 6000 g mol(-1)) were synthesized in water solutions, at the boiling temperature and at different pH values ranging from 7.5 to 12. The Cu(II) complex formation with RLMP was analyzed by UV-vis spectrophotometry and other physicochemical methods. Spectroscopic characterizations (ATR-FTIR, FT-IRIS, and EPR) and spectra-structure correlation of Cu(II)-RLMP complexes were also carried out.
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Niš, Serbia. zak_chem2001@yahoo.com
authors.forename: Zarko
authors.initials: Z
authors.lastname: Mitić
authors.forename: Milorad
authors.initials: M
authors.lastname: Cakić
authors.forename: Goran M
authors.initials: GM
authors.lastname: Nikolić
authors.forename: Ružica
authors.initials: R
authors.lastname: Nikolić
authors.forename: Goran S
authors.initials: GS
authors.lastname: Nikolić
authors.forename: Radmila
authors.initials: R
authors.lastname: Pavlović
authors.forename: Enzo
authors.initials: E
authors.lastname: Santaniello
mesh_terms: D002614:Chelating Agents / Q000737:chemistry; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005936:Glucans / Q000138:chemical synthesis* / Q000737:chemistry; D015591:Microspectrophotometry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002614:Chelating Agents; D056831:Coordination Complexes; D005936:Glucans; D003300:Copper; C009109:pullulan