title: Molecular structure-affinity relationship of natural polyphenols for bovine γ-globulin.
abstract: METHODS
  The aim of this study was to investigate the interaction between polyphenols and bovine γ-globulin.
  
  
  RESULTS
  The relationship between the structural properties of natural polyphenols and their affinities for bovine γ-globulin were investigated by fluorescence titration analysis. Methylation of hydroxyl groups on flavonoids weakened the affinities for γ-globulin by 1.20-38.0 times. Hydroxylation on rings A, B, and C of flavonoids also significantly affected the affinity for γ-globulin. Glycosylation of flavonoids slightly affected the affinity depending on the conjugation site and the class of sugar moiety. Hydrogenation of the C2=C3 double bond on flavonoids decreased the binding affinities. Galloylated catechins and catechol-type catechins exhibited higher binding affinities for γ-globulin than non-galloylated and pyrogallol-type catechins. The glycosylation of resveratrol decreased its affinity for γ-globulin.
  
  
  CONCLUSIONS
  The binding process with γ-globulin was strongly influenced by the structural differences of polyphenols.
authors.affiliation: Department of Biology, College of Life & Environment Science, Shanghai Normal University, Shanghai, P.R. China. jianboxiao@yahoo.com
authors.forename: Jianbo
authors.initials: J
authors.lastname: Xiao
authors.forename: Guoyin
authors.initials: G
authors.lastname: Kai
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Chunxi
authors.initials: C
authors.lastname: Liu
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Xu
authors.forename: Koichiro
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D002392:Catechin / Q000737:chemistry; D002417:Cattle; D005419:Flavonoids / Q000737:chemistry*; D006031:Glycosylation; D006865:Hydrogenation; D006900:Hydroxylation; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols; D011485:Protein Binding; D000077185:Resveratrol; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000737:chemistry; D013329:Structure-Activity Relationship; D005719:gamma-Globulins / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D005719:gamma-Globulins; D002392:Catechin; D000077185:Resveratrol