title: Expression analysis of the Islet-1 gene in the developing and adult gastrointestinal tract.
abstract: LIM-homeodomain genes encode a family of proteins defined by the cysteine-rich protein/protein interacting (Lin-11, Isl-1, and Mec-3) LIM domain and a highly conserved DNA-binding domain. Studies in several organisms have shown that these transcriptional regulators control multiple aspects of embryonic development and are responsible for the pathogenesis of several human diseases. Here we report the expression of Islet-1 (Isl-1) in the gastrointestinal epithelium in developing and adult mice. At embryonic day (E) 9.5-10.5, Isl-1 expression was first detected in the ventral gastric mesenchyme, and expression in the dorsal mesenchyme initiated a few days later. Isl-1 expression was first observed in the gastric epithelium at E13.5 and at E14.5 was restricted to the posterior half of the stomach. In the mature stomach, Isl-1 expression was detected only in subsets of enteroendocrine cells. Furthermore, Isl-1 expression in the intestinal epithelium was first detected at E15.5 and was restricted to subpopulations of enteroendocrine cells in adult mice. These expression analyses suggest that Isl-1 might have an early broad role in stomach and intestinal cells and a secondary role in terminal differentiation and/or maintenance of mature enteroendocrine subtypes in the gastrointestinal epithelium.
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
authors.forename: Pragnya
authors.initials: P
authors.lastname: Das
authors.forename: Catherine Lee
authors.initials: CL
authors.lastname: May
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D053379:Chromogranin A / Q000378:metabolism; D019858:Enteroendocrine Cells / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D054439:Ghrelin / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000196:embryology / Q000254:growth & development; D019394:Ki-67 Antigen / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009928:Organ Specificity; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D012701:Serotonin; D013004:Somatostatin / Q000378:metabolism; D013270:Stomach / Q000196:embryology / Q000254:growth & development; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D053379:Chromogranin A; D005136:Eye Proteins; D054439:Ghrelin; D018398:Homeodomain Proteins; D019394:Ki-67 Antigen; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D012701:Serotonin; D013004:Somatostatin; D015534:Trans-Activators; D014157:Transcription Factors; C498355:Mki67 protein, mouse; C407737:Neurog3 protein, mouse; C000605730:PAX6 protein, human; C000605732:Pax6 protein, mouse; C063843:insulin gene enhancer binding protein Isl-1; C084010:pancreatic and duodenal homeobox 1 protein