title: Estrus induction in Beagle bitches with the GnRH-agonist implant containing 4.7 mg Deslorelin.
abstract: Estrogens, gonadotrophins, dopamine agonists, gonadotrophin releasing hormone (GnRH) and its agonists have been used for estrus induction in bitches. A long acting GnRH agonist implant (4.7 mg Deslorelin; Suprelorin®, Virbac) with a continuous hormone release has been developed for suppression of sexual function in male dogs. In this study we administered the Deslorelin implant placed subcutaneously on the medial side of the leg to induce estrus in 11 anestrous Beagle bitches (group A). 6 Beagle bitches (group B) with a spontaneous estrous cycle were used as controls. The progress of pre-estrus and estrus was documented by behaviour, vaginoscopy, vaginal cytology and progesterone concentration. In group A a bloody vaginal discharge was detected on average 4.8 (range 3-10) d after application of the implant. At this moment implants were removed under local anaesthesia. Pre-estrus lasted for an average of 4.5 d (range 1-12). All bitches showed estrous signs and ovulated. The ovulation took place on day 8.2 (range 4-15) after start of pre-estrus. In group B pre-estrus lasted for 7.5 d (range 6-9), and the mean day of ovulation was day 11 (range 9-13). As a consequence of ovulation, progesterone serum concentrations exceeded 10 ng/ml during or after the time of ovulation in all bitches. All bitches were bred to fertile Beagle stud dogs or inseminated with fresh semen intravaginally. Between days nine and 19 after ovulation all bitches underwent ovariohysterectomy. The uterine horns were flushed and flushes were examined for ova or embryos. The pregnancy rate in group A was 63.6% and in group B 66.7%. Despite the significantly shorter period of pre-estrus a fertile estrus could be induced in 7 out of 11 treated bitches. Induction of a fertile estrus can be achieved with a GnRH-implant-already registered for the use in male dogs-placed subcutaneously on the medial side of the leg.
authors.affiliation: Clinic for Surgery and Reproduction in Small Animals, Veterinary Faculty, Munich, Germany. beate.walter@lmu.de
authors.forename: B
authors.initials: B
authors.lastname: Walter
authors.forename: C
authors.initials: C
authors.lastname: Otzdorff
authors.forename: N
authors.initials: N
authors.lastname: Brugger
authors.forename: J
authors.initials: J
authors.lastname: Braun
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001947:Breeding / Q000379:methods; D004285:Dogs; D004971:Estrus / Q000187:drug effects*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D008297:Male; D010062:Ovulation Induction / Q000662:veterinary; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood; D017329:Triptorelin Pamoate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; C066438:deslorelin