title: The role of exon shuffling in shaping protein-protein interaction networks.
abstract: BACKGROUND
  Physical protein-protein interaction (PPI) is a critical phenomenon for the function of most proteins in living organisms and a significant fraction of PPIs are the result of domain-domain interactions. Exon shuffling, intron-mediated recombination of exons from existing genes, is known to have been a major mechanism of domain shuffling in metazoans. Thus, we hypothesized that exon shuffling could have a significant influence in shaping the topology of PPI networks.
  
  
  RESULTS
  We tested our hypothesis by compiling exon shuffling and PPI data from six eukaryotic species: Homo sapiens, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans, Cryptococcus neoformans and Arabidopsis thaliana. For all four metazoan species, genes enriched in exon shuffling events presented on average higher vertex degree (number of interacting partners) in PPI networks. Furthermore, we verified that a set of protein domains that are simultaneously promiscuous (known to interact to multiple types of other domains), self-interacting (able to interact with another copy of themselves) and abundant in the genomes presents a stronger signal for exon shuffling.
  
  
  CONCLUSIONS
  Exon shuffling appears to have been a recurrent mechanism for the emergence of new PPIs along metazoan evolution. In metazoan genomes, exon shuffling also promoted the expansion of some protein domains. We speculate that their promiscuous and self-interacting properties may have been decisive for that expansion.
authors.affiliation: Ludwig Institute for Cancer Research, São Paulo Branch, Brazil.
authors.forename: Douglas V
authors.initials: DV
authors.lastname: Cancherini
authors.forename: Gustavo S
authors.initials: GS
authors.lastname: França
authors.forename: Sandro J
authors.initials: SJ
authors.lastname: de Souza
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics*; D006801:Humans; D011485:Protein Binding / Q000235:genetics*; D025941:Protein Interaction Mapping; D020033:Protein Isoforms / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics*; D011506:Proteins / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D020033:Protein Isoforms; D011506:Proteins