title: Structures of the SEp22 dodecamer, a Dps-like protein from Salmonella enterica subsp. enterica serovar Enteritidis.
abstract: The crystal structure of SEp22, a DNA-binding protein from starved cells from Salmonella enterica subsp. enterica serovar Enteritidis, has been determined in two forms: the native state at 1.25 Å resolution and an iron-soaked form at 1.30 Å resolution. The SEp22 protomers form a dodecameric shell with 23 symmetry and a single iron ion per protomer was found at the ferroxidase centre in the iron-soaked form. Along the threefold axes of the 23 symmetry, hydrophilic Asp channels that consist of Asp146 were found. Iron ions may flow into the cavity of the dodecameric shell through the Asp channels.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Asahina
authors.forename: Shohei
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Shimizu
authors.forename: Umeharu
authors.initials: U
authors.lastname: Ohto
authors.forename: Shuji
authors.initials: S
authors.lastname: Noguchi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Satow
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D007501:Iron / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012477:Salmonella enteritidis / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C083911:DPS protein, Bacteria; D007501:Iron