title: [Cloning and expression of a fragment of Corylus heterophylla 1 and identifying its immunologic competence].
abstract: OBJECTIVE
  To clone, express, and identify a fragment of Cor h 1 from Corylus heterophylla.
  
  
  METHODS
  Through bioinformatics predication, the antigenic epitope of Cor h 1 was selected. A fragment gene of Cor h 1 was amplified by PCR and cloned into pMD18-T vector for sequencing. Then the fragment gene was sub cloned into pET-32a expression vector for expression, and then purified by metal (Ni(2+);) chelating affinity chromatography. The immunogenicity was tested by Western blot.
  
  
  RESULTS
  The length of the fragment gene was 243 bp, coding 81 amino acids; the relative molecular mass of recombinant protein was 9 000. And the fragment of Cor h 1 was mainly expressed as soluble protein, purified protein has good immunogenicity.
  
  
  CONCLUSIONS
  The fragment gene of Cor h 1 was successfully cloned and expressed in this study, and the recombinant protein possessed good IgE-binding capacity.
authors.affiliation: Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. lina475300@163.com
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Hu
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Liu
authors.forename: Si-li
authors.initials: SL
authors.lastname: Lin
authors.forename: Xin-ping
authors.initials: XP
authors.lastname: Luo
authors.forename: Zhong
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D000595:Amino Acid Sequence; D002846:Chromatography, Affinity / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D031211:Corylus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D021184:Nut Hypersensitivity / Q000276:immunology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D000485:Allergens; D010940:Plant Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E