title: Microarray analysis of gene expression in disk abalone Haliotis discus discus after bacterial challenge.
abstract: In this study, we investigated the gene expression profiling of disk abalone, Haliotis discus discus challenged by a mixture of three pathogenic bacteria Vibrio alginolyticus, Vibrio parahemolyticus, and Listeria monocytogenes using a cDNA microarray. Upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels ≥2 or ≤2 -fold in gills and digestive tract, respectively. There were 46 tissue-specific transcripts that up-regulated specifically in the digestive tract. In contrast, only 13 transcripts showed gill-specific up-regulation. Quantitative real-time PCR was performed to verify microarray data and results revealed that candidate genes namely Krüppell-like factor (KLF), lachesin, muscle lim protein, thioredoxin-2 (TRx-2), nuclear factor interleukin 3 (NFIL-3) and abalone protein 38 were up-regulated. Also, our results further indicated that bacteria challenge may activate the transcription factors or their activators (Krüppell-like factor, inhibitor of NF-κB or Ik-B), inflammatory cytokines (IL-3 regulated protein, allograft inflammatory factor), other cytokines (IFN-44-like protein, SOCS-2), antioxidant enzymes (glutathione-S-transferase, thioredoxin-2 and thioredoxin peroxidase), and apoptosis-related proteins (TNF-α, archeron) in abalone. The identification of immune and stress response genes and their expression profiles in this microarray will permit detailed investigation of the stress and immune responses of abalone genes.
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Special Self-Governing Province 690-756, Republic of Korea.
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Chamilani
authors.initials: C
authors.lastname: Nikapitiya
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
authors.forename: Youngdeuk
authors.initials: Y
authors.lastname: Lee
authors.forename: Ilson
authors.initials: I
authors.lastname: Whang
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology*; D016000:Cluster Analysis; D041981:Gastrointestinal Tract / Q000276:immunology; D049851:Gastropoda / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005880:Gills / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha