title: Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein.
abstract: The naturally split Npu DnaE intein can be used for ligation of an exogenous polypeptide to membrane proteins on living cells. No reducing agents or other factors are required. The approach is rapid and virtually traceless, because the intein removes itself during the reaction.
authors.affiliation: TU Dortmund University, Faculty of Chemistry, Otto-Hahn-Str. 6, D-44227 Dortmund, Germany.
authors.forename: Tulika
authors.initials: T
authors.lastname: Dhar
authors.forename: Henning D
authors.initials: HD
authors.lastname: Mootz
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004258:DNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D058851:GPI-Linked Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D047668:Inteins; D051379:Mice; D046937:Nostoc / Q000201:enzymology; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020040:Trans-Splicing
chemical_list: D058851:GPI-Linked Proteins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III