title: The label-free unambiguous detection and symbolic display of single nucleotide polymorphisms on DNA origami.
abstract: Single nucleotide polymorphisms (SNPs) are the most common genetic variation in the human genome. Kinetic methods based on branch migration have proved successful for detecting SNPs because a mispair inhibits the progress of branch migration in the direction of the mispair. We have combined the effectiveness of kinetic methods with atomic force microscopy of DNA origami patterns to produce a direct visual readout of the target nucleotide contained in the probe sequence. The origami contains graphical representations of the four nucleotide alphabetic characters, A, T, G and C, and the symbol containing the test nucleotide identity vanishes in the presence of the probe. The system also works with pairs of probes, corresponding to heterozygous diploid genomes.
authors.affiliation: Department of Chemistry, New York University, New York, New York 10003, USA.
authors.forename: Hari K K
authors.initials: HK
authors.lastname: Subramanian
authors.forename: Banani
authors.initials: B
authors.lastname: Chakraborty
authors.forename: Ruojie
authors.initials: R
authors.lastname: Sha
authors.forename: Nadrian C
authors.initials: NC
authors.lastname: Seeman
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D004247:DNA