title: Natriuretic peptide system: a link between fat mass and cardiac hypertrophy and hypertension in fat-fed female rats.
abstract: The present study was designed to develop an animal model of hypertension and cardiac hypertrophy associated with obesity in female rats. Furthermore, we studied the involvement of the natriuretic peptide system in the mechanisms of these conditions. Obesity was induced in Wistar rats by a high fat diet and ovariectomy. The rats were divided into four groups: ovariectomized or sham-operated with high-fat diet and ovariectomized or sham-operated with control diet. After 24 weeks of diet, rats were killed, and their tissues were removed. Cardiac atrial natriuretic peptide (ANP), clearance receptor (NPr-C) gene expression was determined by PCR. ANP concentrations were measured in plasma. Ovariectomized fat-fed rats (OF) showed increased body weight, visceral fat depot and blood pressure and decreased sodium excretion compared to other groups. Also, these rats showed higher heart-to-body weight and cell diameters of ventricular cardiomyocytes and lower cardiac ANP mRNA and plasma ANP than the control group. The adipocyte and renal NPr-C mRNA of OF rats were higher than the control group. These data showed that combined ovariectomy and high fat diet elicited obesity, hypertension and cardiac hypertrophy. These results suggest that the impairment of the natriuretic peptide system may be one of the mechanisms involved not only in development of hypertension but also in cardiac hypertrophy associated with obesity in ovariectomized rats.
authors.affiliation: Multidisciplinary Institute of Health, Federal University of Bahia, Av. Olívia Flores 3000, CEP: 45055-090, Vitória da Conquista, Bahia, Brazil.
authors.forename: Everaldo Nery
authors.initials: EN
authors.lastname: de Andrade
authors.forename: Gleisy Kelly Neves
authors.initials: GK
authors.lastname: Gonçalves
authors.forename: Thiago Henrique Caldeira
authors.initials: TH
authors.lastname: de Oliveira
authors.forename: Caroline Silva Dos
authors.initials: CS
authors.lastname: Santos
authors.forename: Clarissa Leal Silva E
authors.initials: CL
authors.lastname: Souza
authors.forename: Luciana Barbosa
authors.initials: LB
authors.lastname: Firmes
authors.forename: Amélia Cristina Mendes
authors.initials: AC
authors.lastname: de Magalhães
authors.forename: Telma de Jesus
authors.initials: Tde J
authors.lastname: Soares
authors.forename: Adelina Martha Dos
authors.initials: AM
authors.lastname: Reis
authors.forename: Najara de Oliveira
authors.initials: Nde O
authors.lastname: Belo
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood* / Q000235:genetics; D001794:Blood Pressure; D006332:Cardiomegaly / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D004032:Diet; D004041:Dietary Fats / Q000009:adverse effects / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006321:Heart / Q000503:physiopathology; D006973:Hypertension / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D050152:Intra-Abdominal Fat / Q000473:pathology; D009765:Obesity / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D009929:Organ Size; D010052:Ovariectomy / Q000009:adverse effects; D010053:Ovary; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000097:blood* / Q000235:genetics; D012964:Sodium / Q000652:urine
chemical_list: D004041:Dietary Fats; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D012964:Sodium; D017461:Receptors, Atrial Natriuretic Factor