title: The functions of an apple cytosolic malate dehydrogenase gene in growth and tolerance to cold and salt stresses.
abstract: It is well-known that cytosolic NAD-dependent malate dehydrogenase (cyMDH; l-malate:NAD-oxidoreductase; EC 1.1.1.37) is an enzyme crucial for malic acid synthesis in the cytosol. Nothing is known about cyMDH in growth and stress tolerance. Here we characterised the role of the apple cyMDH gene (MdcyMDH, GenBank ID: DQ221207) in growth and tolerance to cold and salt stresses. MdcyMDH transcripts were highly accumulated in vigorously growing apple tissues, organs and suspension cells. In addition, MdcyMDH was sensitive to cold and salt stresses. MdcyMDH overexpression favourably contributed to cell and plant growth and conferred stress tolerance both in the apple callus and tomato. Taken together, our results indicated that MdcyMDH is involved in plant and cell growth as well as the tolerance to cold and salt stresses.
authors.affiliation: State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Yao
authors.forename: Qing-Long
authors.initials: QL
authors.lastname: Dong
authors.forename: Heng
authors.initials: H
authors.lastname: Zhai
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: You
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Hao
mesh_terms: D003080:Cold Temperature; D003600:Cytosol / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008293:Malates / Q000378:metabolism*; D027845:Malus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D009243:NAD / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D055049:Salt Tolerance; D012965:Sodium Chloride; D013312:Stress, Physiological
chemical_list: D008293:Malates; D010940:Plant Proteins; D009243:NAD; D012965:Sodium Chloride; C030298:malic acid; D008291:Malate Dehydrogenase