title: [Molecular identification of human Diphyllobothrium nihonkaiense using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequence].
abstract: Identification of Diphyllobothrium species has been carried out based on their morphology, especially sexual organs. In addition to these criteria, PCR-based identification methods have been developed recently. A 20 year-old Japanese living in Kochi Prefecture passed tapeworm. He was successfully treated with single dose of gastrografin. We examined the morphologic features of the proglottids and eggs using histology and scanning electron microscope. We also analyzed mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of the proglottids. The causative tapeworm species was identified as D. nihonkaiense based on the results of morphologic features and genetic analysis. We discussed the advantage of PCR-based identification methods of Diphyllobothrium species using cox1 sequence in the clinical laboratory.
authors.affiliation: Department of Clinical Laboratory, Kochi Medical School, Kochi University, Nankoku 783-8505, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Ono
authors.forename: Norihito
authors.initials: N
authors.lastname: Morimoto
authors.forename: Masataka
authors.initials: M
authors.lastname: Korenaga
authors.forename: Hideo
authors.initials: H
authors.lastname: Kumazawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Itsu
authors.initials: I
authors.lastname: Kuge
authors.forename: Yoshihumi
authors.initials: Y
authors.lastname: Higashidani
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ogura
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sugiura
mesh_terms: D000328:Adult; D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D003974:Diatrizoate Meglumine / Q000008:administration & dosage; D004169:Diphyllobothriasis / Q000175:diagnosis / Q000188:drug therapy / Q000469:parasitology*; D004170:Diphyllobothrium / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D003576:Electron Transport Complex IV / Q000235:genetics*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D003974:Diatrizoate Meglumine; D003576:Electron Transport Complex IV