title: [Effects of stress on hippocampal Glu-NMDAR pathway in rats and mechanism of zinc protection].
abstract: OBJECTIVE
  To observe the effects of stress on Glu uptake and NMDAR of hippocampus synaptosome in rats with different zinc status.
  
  
  METHODS
  Stress model was established by photoelectric stimulus. The behaviors of rats were tested in open-field case. 3H-L-Glu was taken as radioligand to detect the NMDAR binding. Glu uptake was determined with radioimmunoassay.
  
  
  RESULTS
  Compared with CT rats, ZD rats performed less movement in open-field test, both Bmax of NMDAR and 3H-L-Glu uptake of hippocampus in these rats were significantly decreased. Compared with corresponding non-stressed groups, the stressed groups appeared longer latency and less movement in open-field test. Increased Bmax of NMDAR and decreased 3H-L-Glu uptake were observed in all stressed rats, but only in SZD rats these indices showed statistical difference.
  
  
  CONCLUSIONS
  Abnormal behaviors of rats induced by photoelectric stress were observed in open-field test, which was more serious in zinc deficiency rats. It is supposed that the Glu-NMDAR pathway is involved in the process of stress reaction. As it shows in our experiment, the changes of Bmax of NMDAR and 3H-L-Glu uptake of hippocampus synaptosome seems to be a part of the mechanisms of stress action.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Zhan-hui
authors.initials: ZH
authors.lastname: Geng
authors.forename: Yi-yong
authors.initials: YY
authors.lastname: Cheng
authors.forename: Xiu-ling
authors.initials: XL
authors.lastname: Ma
authors.forename: Shu-tian
authors.initials: ST
authors.lastname: Li
authors.forename: Yong-hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Shi
mesh_terms: D000818:Animals; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D013312:Stress, Physiological; D015032:Zinc / Q000494:pharmacology*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D015032:Zinc