title: Chronological study of cytokine transcription in the spleen and lung of chickens after vaccination with serotype 1 Marek's disease vaccines.
abstract: Transcription of cytokine genes was evaluated in the lung and spleen of chickens vaccinated with various serotype 1 Marek's disease (MD) vaccines. Three vaccine pairs/series, each consisting of one or two high protective (HP) and one low protective (LP) vaccine, were used. Vaccinated chickens had increased transcripts of IFN-γ in the lung and spleen at 3, 5, and 10 days post vaccination (dpv) compared to unvaccinated control chickens. In addition, transient increase of cytokine transcripts (iNOS, IL-1β, IL-18, IL-8, and IL-6 in the lung and iNOS, IL-18, and IL-6 in the spleen) was detected. Compared to chickens vaccinated with LP vaccines, HP vaccinated chickens had increased transcripts of iNOS at 5 dpv but decreased transcripts of IL-6, IL-8, and IL-18 at 10 dpv in the lung. HP vaccinated chickens had increased IFN-γ in the spleen at 3 and at 10 dpv. This study demonstrated that MD vaccines administered subcutaneously elicit a pulmonary immune response and identified differences in the cytokine gene expression between HP and LP vaccinated chickens.
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA. Isabel Gimeno@ncsu.edu
authors.forename: Isabel M
authors.initials: IM
authors.lastname: Gimeno
authors.forename: Aneg L
authors.initials: AL
authors.lastname: Cortes
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D008381:Herpesvirus 2, Gallid / Q000276:immunology; D008168:Lung / Q000276:immunology*; D008380:Marek Disease / Q000276:immunology / Q000517:prevention & control*; D022501:Marek Disease Vaccines / Q000276:immunology*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology*; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary; D019562:Viral Load
chemical_list: D016207:Cytokines; D022501:Marek Disease Vaccines; D052247:Nitric Oxide Synthase Type II