title: Evaluation of serological diagnostic methods for the 2009 pandemic influenza A (H1N1) virus.
abstract: Serology improves influenza diagnosis by capturing cases missed by reverse transcriptase PCR (RT-PCR). We prospectively evaluated microneutralization and hemagglutination inhibition assays for 2009 influenza A (H1N1) virus diagnosis among 24 RT-PCR-confirmed cases and 98 household contacts. Compared to hemagglutination inhibition, microneutralization demonstrated a higher level of concordance with RT-PCR (kappa = 0.69 versus kappa = 0.60) and greater sensitivity (83% versus 71%; P = 0.016).
authors.affiliation: Infectious Disease Research Center of the CHUQ-CHUL and Laval University, Quebec City, Quebec, Canada.
authors.forename: Jesse
authors.initials: J
authors.lastname: Papenburg
authors.forename: Mariana
authors.initials: M
authors.lastname: Baz
authors.forename: Marie-Ève
authors.initials: MÈ
authors.lastname: Hamelin
authors.forename: Chantal
authors.initials: C
authors.lastname: Rhéaume
authors.forename: Julie
authors.initials: J
authors.lastname: Carbonneau
authors.forename: Manale
authors.initials: M
authors.lastname: Ouakki
authors.forename: Isabelle
authors.initials: I
authors.lastname: Rouleau
authors.forename: Gaston
authors.initials: G
authors.lastname: De Serres
authors.forename: Guy
authors.initials: G
authors.lastname: Boivin
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D008875:Middle Aged; D009500:Neutralization Tests / Q000379:methods; D011446:Prospective Studies; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D055815:Young Adult
chemical_list: D012367:RNA, Viral