title: A bio-activity guided in vitro pharmacokinetic method to improve the quality control of Chinese medicines, application to Si Wu Tang.
abstract: The purpose of this study was to demonstrate the feasibility of using a bio-activity guided in vitro pharmacokinetic (BAPK) method in identifying relevant (absorbable and bioactive) markers for quality control (QC) of Chinese medicines (CM), using Si Wu Tang (SWT), a popular CM for women's health, as an example. A stepwise BAPK approach was utilized for relevant marker determination and evaluating of six SWT products: (1) data mining to identify active components of SWT, (2) quantification of the identified active components in each SWT product, (3) determination of in vitro dissolution and metabolism of the components under simulated gastrointestinal conditions, (4) identification of absorbable components or marker(s) via in vitro Caco-2 cell model, (5) stability testing of the permeable marker(s). Our results showed considerable variations in the amount of active components in different SWT products. Of the nine active components identified from data mining, three (ferulic acid, ligustilide, senkyunolide A) were found to be well permeated and stable over three months. Paeoniflorin, the marker designated by Chinese Pharmacopoeia, was poorly permeable and thus could not be considered a relevant marker for SWT. Our preliminary evaluation of the BAPK method appears to be feasible and may offer as a useful approach for identifying relevant markers of other TCM products in the future.
authors.affiliation: School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Siukwan
authors.initials: S
authors.lastname: Wo
authors.forename: Clara B S
authors.initials: CB
authors.lastname: Lau
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.forename: Vincent H L
authors.initials: VH
authors.lastname: Lee
authors.forename: Moses S S
authors.initials: MS
authors.lastname: Chow
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zuo
mesh_terms: D000327:Adsorption; D018938:Caco-2 Cells; D002853:Chromatography, Liquid; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics / Q000592:standards; D005240:Feasibility Studies; D006801:Humans; D008954:Models, Biological; D011786:Quality Control; D012995:Solubility; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; C403258:si-wu-tang