title: Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain.
abstract: The nuclear transport factor 2-like (NTF2-like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging-drop vapour-diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a=b=89.84, c=70.02 Å. The crystals contained one molecule per asymmetric unit, with an estimated solvent content of 56%. Initial phases were obtained by molecular replacement.
authors.affiliation: Biostructural Research, Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Vognsen
authors.forename: Ingvar Rúnar
authors.initials: IR
authors.lastname: Möller
authors.forename: Ole
authors.initials: O
authors.lastname: Kristensen
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004265:DNA Helicases; D006801:Humans; D008969:Molecular Sequence Data; D000075223:Poly-ADP-Ribose Binding Proteins; D011487:Protein Conformation; D020033:Protein Isoforms / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins
chemical_list: D002352:Carrier Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D020033:Protein Isoforms; D000067776:RNA Recognition Motif Proteins; D004265:DNA Helicases; C116070:G3BP1 protein, human; D020365:RNA Helicases