title: MRI and histologic analysis of collagen type II sponge on repairing the cartilage defects of rabbit knee joints.
abstract: There are limited treatment options for cartilage defects in clinical practice because of the lack of suitable biomaterials. Here, we evaluated the effects of collagen type II sponge on the articular cartilage repairing process using a cartilage injury of a rabbit knee joint model. We showed that the home-made collagen type II sponges appeared to have a suitable pore size of 93.26 ± 38.4 μm for chondrocyte growth. MRI with H&E staining results demonstrated that the effusion absorption in the collagen type II sponge treated group was quicker than that of the control group. Moreover, sporadic cartilage signals first appeared at 6 weeks in the collagen type II sponge treated group. Safranin O staining and immunohistochemical analysis confirmed that the newly formed cartilage expresses glycosaminoglycan and type II collagen matrix. Using Sirius red polarized light staining, we showed that the newly formed cartilage-like areas from the collagen type II treated group are significantly greater than those of the control group. Taken together, our data demonstrated that the home-made collagen type II sponge is able to promote cartilage repair in the cartilage injury of a rabbit knee joint model.
authors.affiliation: Guangzhou Institute of Traumatic Surgery, the Fourth Affiliated Hospital of Medical College of Jinan University, Guangzhou 510220, Guangdong, People's Republic of China. honghchen@21cn.com
authors.forename: Honghui
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.forename: Yingyang
authors.initials: Y
authors.lastname: Liao
authors.forename: Xuwen
authors.initials: X
authors.lastname: Zeng
authors.forename: Peihong
authors.initials: P
authors.lastname: Liang
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.forename: Jianrong
authors.initials: J
authors.lastname: Tan
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Liang
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000293:injuries* / Q000601:surgery; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000627:therapeutic use*; D006025:Glycosaminoglycans / Q000096:biosynthesis; D007719:Knee Joint / Q000601:surgery*; D008279:Magnetic Resonance Imaging; D011817:Rabbits; D015503:Surgical Sponges; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans