title: Characterization of canine BCL6 cDNA and detection systems for its protein expression.
abstract: BCL6 is known to be a key molecule in germinal center (GC) formation of lymph nodes, and its expression profiles have been implicated in the prognosis of diffuse large B-cell lymphoma in humans. The present study was carried out to characterize canine BCL6 cDNA and to indicate the technical methods for detection of the BCL6 protein in dog tissues. The deduced amino acid sequence of canine BCL6 showed close homology to that of human BCL6 (96.3%), especially in the zinc-finger motifs and POZ (poxvirus and zinc finger) domain with complete identity. Immunoblot analysis of a canine lymph node with an anti-human BCL6 monoclonal antibody revealed a band of 80 kDa. Immunohistochemical staining using the same antibody produced positive reactions in the cells exclusively localized in the GC of a canine lymph node. This study will be useful for the molecular classification of canine B-cell lymphomas with different prognoses.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sato
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanemoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004285:Dogs / Q000235:genetics* / Q000276:immunology; D018858:Germinal Center / Q000276:immunology*; D015151:Immunoblotting / Q000662:veterinary; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D004268:DNA-Binding Proteins