Ossification vesicles with calcium phosphate in the eyes of the insect Copium teucrii (Hemiptera: Tingidae).
Arthropod eyes are built of repeating units named ommatidia. Each single ommatidium unit contains a cluster of photoreceptor cells surrounded by support cells and pigment cells. The insect Copium eye ommatidia include additional calcium-phosphate deposits, not described in insects to date, which can be examined today using a combined set of modern microscopy and spectroscopy techniques. Teucrium gnaphalodes L'Her plants, growing in central Spain, develop galls induced by Copium insects. A survey of C. teucrii adult specimens resulted in surprising environmental scanning electron microscopy (ESEM) images, showing that their bright red eyes contain a calcium-phosphate mineralization. A complete survey of Copium eye specimens was performed by ESEM using energy-dispersive spectroscopy, backscattered electron detector and cathodoluminescence (CL) probes, field emission scanning electron microscopy, micro-Raman spectroscopy, and confocal laser scanning microscopy in order to learn ommatidia features, such as chemical composition, molecular structure, cell membrane, and internal ommatidium eye fluids and calcium-phosphate distribution deposits. The CL panchromatic images distinguish between the calcium-phosphate ommatidium and calcium-phosphate setae, which are more apatite rich. They show Raman bands attributable to bone tissue apatite biomaterials, such as bone, collagen, lipids, and blood, i.e., peptides, amide-S, amide-II, amide-III, and cytochrome P-450 scc. The chemical composition of both galls and leaves of T. gnaphalodes was determined by gas chromatography-mass spectrometry (GC-MS) of their extracts. The spectrometric and microscopic images reveal that the calcium-phosphate mineralization is formed and constrained to Copium ommatidia, which are both matrix vesicles generating mixtures of apatite collagen and operational compound eyes of the insect.
Museo Nacional de Ciencias Naturales (MNCN, CSIC), Madrid, Spain. guinea@mncn.csic.es
Javier C
JC
Garcia-Guinea
Alberto C
AC
Jorge
Laura
L
Tormo
Marta
M
Furio
Elena
E
Crespo-Feo
Virgilio
V
Correcher
Pedro
P
Prado-Herrero
Ana C
AC
Soria
Jesus
J
Sanz
Jose L
JL
Nieves-Aldrey
D000818:Animals; D002130:Calcium Phosphates / Q000378:metabolism*; D005123:Eye / Q000378:metabolism* / Q000648:ultrastructure; D008401:Gas Chromatography-Mass Spectrometry; D006430:Hemiptera / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology*; D022142:Secretory Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman
D002130:Calcium Phosphates; C020243:calcium phosphate