Tumoral tissue specific promoter hypermethylation of distinct tumor suppressor genes in a case with nonsmall cell lung carcinoma: a case report.
OBJECTIVE
Non-small cell lung carcinoma is an aggressive phenomenon and the epigenetical alterations of some tumor supressor genes have been reported for the different tumor types.


METHODS
It is presented a case report concerning a 43 years old male with NSCLC on the lower segment of the right lung. The patient underwent a diag-nostic excisional thin-needle biopsy and after the histological confirmation. We examined the promoter methylation status of some distinct tumor supressor genes in tumoral and blood tissues of the case after sodium bisulfite conversion and DNA amplification with methylation specific multiplex PCR technique. Both tissues were also searched for G to A transitions in codons 12 and 13 of the K-ras proto-oncogene.


RESULTS
Tumor specimen showed fully methyl pattern profiles for the SFRP2, p16, DAPK1 and partially hyper-methylated profile for the p53 and MGMT genes in this case with non-small lung carci-noma. Blood speicemen showed normal hypomethylated profiles for all studied TS genes. The K-ras proto-oncogene was in normal structure both in blood and tumoral spiecemens that examined.


CONCLUSIONS
Results indicate that genes exhibit tumor suppressor activi-ties in blood, but exhibit epigenetic inactivation in carcinoma cell. These findings strongly support the hypothesis that epigenetic mechanisms may play an important role in the non-small cell lung carcinogenesis in human.
Department of Thorax Diseases, Faculty of Medicine, Cumhuriyet University 58140 Sivas/Turkey.
Sulhattin
S
Arslan
Tamer
T
Dogan
Binnur
B
Koksal
Malik Ejder
ME
Yildirim
Cesur
C
Gumus
Sahenda
S
Elagoz
Ibrahim
I
Akkurt
Oztürk
O
Ozdemir
Br J Cancer. 2008 Mar 25;98(6):1147-56
18283316
Br J Haematol. 2008 Apr;141(2):179-87
18318772
Presse Med. 1994 Feb 12;23(6):291-7
8208681
Semin Cancer Biol. 2004 Dec;14(6):427-32
15489135
World J Gastroenterol. 2007 Feb 14;13(6):950-4
17352030
Cancer Res. 2003 Mar 1;63(5):1114-21
12615730
J Cell Biol. 2006 Feb 13;172(4):619-31
16476779
Int J Cancer. 2007 Sep 1;121(5):1028-35
17443492
J Med Genet. 2001 May;38(5):285-303
11333864
Exp Lung Res. 1998 Jul-Aug;24(4):463-79
9659578
Cancer Res. 1999 Feb 15;59(4):793-7
10029064
Cancer Res. 1995 Oct 15;55(20):4525-30
7553621
Semin Oncol. 2001 Apr;28(2 Suppl 4):3-13
11479891
Exp Toxicol Pathol. 2000 Aug;52(4):317-22
10987184
Oncogene. 2007 Jul 12;26(32):4699-713
17297461
Clin Cancer Res. 2006 Dec 1;12(23):6989-97
17145819
Clin Cancer Res. 2005 Feb 1;11(3):1219-25
15709192
CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66
17237035
J Pathol. 2002 Jan;196(1):1-7
11748635