Characterization of species C human adenovirus serotype 6 (Ad6).
Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than the others which may explain differences in virus infectivity in vitro, but not in the liver. Comparison of hexon hypervariable regions (HVRs) suggests that the higher transduction by Ad5 and 6 as compared to Ad1 and 2 may be related to differences in charge and length.
Division of Infectious Diseases, Department of Internal Medicine, Translational Immunovirology and Biodefense Program, Mayo Clinic, 200 First Street SW, Rochester, MN 55902, USA.
Eric A
EA
Weaver
Mathew L
ML
Hillestad
Reeti
R
Khare
Donna
D
Palmer
Philip
P
Ng
Michael A
MA
Barry
D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000254:growth & development*; D000595:Amino Acid Sequence; D000818:Animals; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D017930:Genes, Reporter; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology; D008156:Luciferases / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009132:Muscles / Q000473:pathology / Q000821:virology; D010802:Phylogeny; D012376:Rodent Diseases / Q000473:pathology / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014161:Transduction, Genetic; D051598:Whole Body Imaging / Q000379:methods
D004279:DNA, Viral; D008156:Luciferases
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