Shifted-excitation Raman difference spectroscopy for in vitro and in vivo biological samples analysis.
The contamination of the Raman scattering signal with luminescence is a well-known problem when dealing with biological media excited by visible light. The viability of the shifted-excitation Raman difference spectroscopy (SERDS) technique for luminescence suppression on Raman spectra of biological samples was studied in this work. A tunable Lithrow-configuration diode laser (λ = 785 and 830 nm) coupled (directly or by optical fiber) to a dispersive Raman spectrometer was employed to study two sets of human tissues (tooth and skin) in order to determine the set of experimental parameters suitable for luminescence rejection. It was concluded that systematic and reproducible spectra of biological interest can be acquired by SERDS.
Mário Augusto
MA
da Silva Martins
Dayana Gonçalves
DG
Ribeiro
Edson Aparecido
EA
Pereira Dos Santos
Airton Abrahão
AA
Martin
Adriana
A
Fontes
Herculano
H
da Silva Martinho
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