Polyaspartamide-graft-polymethacrylate nanoparticles for doxorubicin delivery.
A new PHEA-IB-PMANa(+) copolymer has been synthesized and its pH-induced self-assembly has been investigated in an aqueous medium. PHEA-IB-PMANa+ formed nanoparticles with diameters from 25 to 50 nm upon protonation of the carboxylic acid moieties dislocated along the grafted polymethacrylate sodium salt side chains. The physico-chemical characterization of the nanoparticles was performed using light scattering, zeta-potential measurements, SEM, and AFM. Doxorubicin-loaded nanoparticles were prepared and drug release profiles were evaluated under conditions mimicking physiological media. A biological characterization was carried out by testing the cytotoxicity on Caco-2 cells, and cellular uptake on mouse monocyte macrophage (J774 A.1) and Caco-2 cells.
Department of Chimica e Tecnologie Farmaceutiche, Laboratory of Biocompatible Polymers, University of Palermo, Via Archirafi, 32 90129 Palermo, Italy.
Mariano
M
Licciardi
Gennara
G
Cavallaro
Mauro
M
Di Stefano
Calogero
C
Fiorica
Gaetano
G
Giammona
D000818:Animals; D018938:Caco-2 Cells; D016923:Cell Death / Q000187:drug effects; D055598:Chemical Phenomena / Q000187:drug effects; D002850:Chromatography, Gel; D004317:Doxorubicin / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D008027:Light; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D012542:Scattering, Radiation; D012996:Solutions
D010455:Peptides; D011109:Polymethacrylic Acids; D012996:Solutions; C030613:polymethacrylic acid; C017645:polyaspartate; D004317:Doxorubicin