Ultrasensitive scanometric strategy for detection of matrix metalloproteinases using a histidine tagged peptide-Au nanoparticle probe.
A simple scanometric strategy was developed for ultrasensitive assay of matrix metalloproteinases based on their discriminatory and proteolytic activity by integrating a newly designed peptide-gold nanoparticle probe, a nitrilotriacetic acid modified chip and silver signal amplification.
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China.
Wei
W
Cheng
Yunlong
Y
Chen
Feng
F
Yan
Lin
L
Ding
Shijia
S
Ding
Huangxian
H
Ju
Yibing
Y
Yin
D000595:Amino Acid Sequence; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000378:metabolism*; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008969:Molecular Sequence Data; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D013379:Substrate Specificity
D010455:Peptides; D006639:Histidine; D006046:Gold; D020782:Matrix Metalloproteinases; D020783:Matrix Metalloproteinase 7; D009571:Nitrilotriacetic Acid