In situ apoptosis of adaptive immune cells and the cellular escape of rabies virus in CNS from patients with human rabies transmitted by Desmodus rotundus.
The aim of the current study was to investigate the apoptosis of neurons, astrocytes and immune cells from human patients that were infected with rabies virus by vampire bats bite. Apoptotic neurons were identified by their morphology and immune cells were identified using double immunostaining. There were very few apoptotic neurons present in infected tissue samples, but there was an increase of apoptotic infiltrating CD4+ and TCD8+ adaptive immune cells in the rabies infected tissue. No apoptosis was present in NK, macrophage and astrocytes. The dissemination of the human rabies virus within an infected host may be mediated by viral escape of the virus from an infected cell and may involve an anti-apoptotic mechanism, which does not kill the neuron or pro-apoptosis of TCD4+ and TCD8+ lymphocytes and which allows for increased proliferation of the virus within the CNS by attenuation of the adaptive immune response.
Faculdade de Medicina da Universidade de São Paulo, Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, Departamento de Patologia, São Paulo, Brazil. elaineraniero@usp.br
Elaine Raniero
ER
Fernandes
Heitor Franco
HF
de Andrade
Carmen Lúcia Penteado
CL
Lancellotti
Juarez Antônio Simões
JA
Quaresma
Samia
S
Demachki
Pedro Fernando
PF
da Costa Vasconcelos
Maria Irma Seixas
MI
Duarte
D000293:Adolescent; D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001733:Bites and Stings; D020805:Central Nervous System Viral Diseases / Q000473:pathology / Q000821:virology; D002648:Child; D002685:Chiroptera / Q000821:virology; D018792:Encephalitis, Viral / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000276:immunology / Q000473:pathology; D011818:Rabies / Q000276:immunology* / Q000401:mortality / Q000635:transmission / Q000821:virology*; D011820:Rabies virus / Q000276:immunology*