Expression of mitochondrial uncoupling protein UCP2 in the brain of rats after hippocampal injury inflicted by kainic acid.
The expression of mitochondrial protein UCP2 and cytochrome C-oxidase subunit III genes in the hippocampus and prefrontal cortex was evaluated by real-time PCR 3 and 7 days after microinjection of kainic acid into the dorsal hippocampus. In contrast to cytochrome C-oxidase subunit III mRNA, the level of UCP2 mRNA in the hippocampus increased 1 week after microinjection of kainic acid. The expression of both genes in the prefrontal cortex did not differ from the control. Presumably, activation of UCP2 synthesis in hippocampal injury indicates the neuroprotective effects of this protein.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
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Lebedev
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Arkhipov
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D003576:Electron Transport Complex IV / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D007473:Ion Channels / Q000378:metabolism*; D007608:Kainic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008845:Microinjections; D024101:Mitochondrial Proteins / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071246:Uncoupling Protein 2
D017931:DNA Primers; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D003576:Electron Transport Complex IV; D007608:Kainic Acid