Media optimization studies for Serratiopeptidase production from Serratia marcescens ATCC 13880.
Production of an anti-inflammatory enzyme serratiopeptidase by fermentation with Serratia marcescens ATCC 13880 was studied to ascertain optimal nutritional conditions for large scale production. To study biosynthesis and production of serratiopeptidase by Serratia marcescens ATCC 13880, different physicochemical parameters were studied and optimized. The optimized medium contain, (g/l) glycerine 10.0, maltose 10.0 as carbon source, peptone 10.0 as organic nitrogen source, ammonium sulphate 10.0 as inorganic nitrogen source, dihydrogen phosphate 10.0, sodium bicarbonate 10.0, sodium acetate 10.0 as inorganic salt source, ascorbic acid 10.0 as stabilizer, distilled water 1000 ml and the optimized fermentation conditions were pH 7.0, temperature 37 degrees C and duration 24 hr. The modified fermentation medium produced 27.36 IU/ml of serratiopeptidase compared to 17.97 IU/ml in basal medium and the molecular weight of the purified serratiopeptidase was found to be 52 kD.
Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411018. badheravi@rediffmail.com
Ravindra V
RV
Badhe
Rabindra K
RK
Nanda
Manasi B
MB
Kulkarni
Mayur N
MN
Bhujbal
Pradeep S
PS
Patil
Sonali R
SR
Badhe
D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D012706:Serratia marcescens / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature
D003470:Culture Media; D010447:Peptide Hydrolases; D009584:Nitrogen; C022640:serratiopeptidase