[Molecular identification for the asexual stage of Taishan cordyceps].
OBJECTIVE
From gene lever identified the asexual stage of Taishan cordyceps.


METHODS
Using rDNA ITS regions as molecular targets, PCR amplification, sequencing, and comparing with the other ITS region sequences of known.


RESULTS
Through the Blast search of GenBank, the ITS sequence is same as the sequence of Paecilomyces tenuipes (100% ).


CONCLUSIONS
The asexual stage of Taishan cordyceps was proved through molecular biology method for the first time. The results showed that the asexual stage of Taishan cordyceps. should be Isariafarinosa, not be Hirsutella sinensis.
Taishan Medical University, Taian 271016, China. zhaoxuem@eyou.com
Xue-Mei
XM
Zhao
Hui
H
Zhang
Hong-Xia
HX
Jiang
Xian-Zhong
XZ
Zhang
Yan-You
YY
Su
Yanfei
Y
Wang
D001483:Base Sequence; D002681:China; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006999:Hypocreales / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer