Effects of single nucleotide changes on the binding and activity of RNA aptamers to human papillomavirus 16 E7 oncoprotein.
A virally-encoded oncoprotein (E7 from human papillomavirus 16, involved in the initiation of cell transformation) was the target for RNA aptamer development by the process of systematic evolution of ligands by exponential enrichment (SELEX). A number of aptamers were identified, one of which was shown to inhibit the interaction between E7 and its major binding partner, pRb. Aptamers with very similar sequences (more than 92% similarity in the random regions) did not share this activity. This study demonstrates the potential of aptamers to be highly specific, with small differences in aptamer sequence having profound effects on function.
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.
Clare
C
Nicol
David H J
DH
Bunka
G Eric
GE
Blair
Nicola J
NJ
Stonehouse
D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D019020:Directed Molecular Evolution; D052162:Human papillomavirus 16; D006801:Humans; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000737:chemistry / Q000235:genetics; D050725:Papillomavirus E7 Proteins / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D012641:Selection, Genetic
D052157:Aptamers, Nucleotide; D009711:Nucleotides; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16