Laser microperforated biodegradable microbial polyhydroxyalkanoate substrates for tissue repair strategies: an infrared microspectroscopy study.
Flexible and biodegradable film substrates prepared by solvent casting from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) solutions in chloroform were microperforated by ultraviolet laser ablation and subsequently characterized using infrared (IR) microspectroscopy and imaging techniques and scanning electron microscopy (SEM). Both transmission synchrotron IR microspectroscopy and attenuated total reflectance microspectroscopy measurements demonstrate variations in the polymer at the ablated pore rims, including evidence for changes in chemical structure and crystallinity. SEM results on microperforated PHBHV substrates after cell culture demonstrated that the physical and chemical changes observed in the biomaterial did not hinder cell migration through the pores.
Instituto de Ciencia y Tecnología de Polímeros, CSIC, Madrid, Spain. gary@ictp.csic.es
Gary
G
Ellis
Pilar
P
Cano
María
M
Jadraque
Margarita
M
Martín
Laura
L
López
Teresa
T
Núñez
Enrique
E
de la Peña
Carlos
C
Marco
Leoncio
L
Garrido
D001672:Biocompatible Materials / Q000737:chemistry*; D002465:Cell Movement; D006801:Humans; D015603:Keratinocytes; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing; D000094122:Polyhydroxybutyrates
D001672:Biocompatible Materials; D011091:Polyesters; D054813:Polyhydroxyalkanoates; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates