[Level of DNA adduct in lung cancer tissues and its influencing factors].
BACKGROUND
To detect DNA adduct in lung cancer tissues and analyze the factors influencing formation of DNA adduct.


METHODS
DNA was extracted from the tumor tissues derived from 34 patients with lung cancer. DNA adducts were analyzed using 32P-postlabeling method with P 1 nuclear modification.


RESULTS
DNA adduct level was significantly higher in smoking patients than that in non smoking patients(P < 0.05). DNA adduct level was related to the histological classification, degree of cell differentiation and lymph node metastasis, but not to age and sex.


CONCLUSIONS
DNA adduct level might be used as a potential marker to estimate malignant degree for lung cancer.
Department of Medical Oncology, Tangshan Worker Hospital, Hebei Medical University, Tangshan, Hebei 063000, P.R.China.
Zhi
Z
Zhang
Xuemei
X
Zhang
Wenmin
W
Yang
Zhanzhao
Z
Fu