Quantitative Photoacoustic Image Reconstruction using Fluence Dependent Chromophores.
In biomedical photoacoustic imaging the images are proportional to the absorbed optical energy density, and not the optical absorption, which makes it difficult to obtain a quantitatively accurate image showing the concentration of a particular absorbing chromophore from photoacoustic measurements alone. Here it is shown that the spatially varying concentration of a chromophore whose absorption becomes zero above a threshold light fluence can be estimated from photoacoustic images obtained at increasing illumination strengths. This technique provides an alternative to model-based multiwavelength approaches to quantitative photoacoustic imaging, and a new approach to photoacoustic molecular and functional imaging.
Department of Medical Physics and Bioengineering, University College London, London, WC1E 6BT, UK. bencox@mpb.ucl.ac.uk.
B T
BT
Cox
J G
JG
Laufer
P C
PC
Beard
Chem Rev. 2000 Jun 14;100(6):1973-2012
11749281
Med Phys. 1993 Mar-Apr;20(2 Pt 1):299-309
8497214
Appl Opt. 2006 Mar 10;45(8):1866-75
16572706
J Biomed Opt. 2010 Mar-Apr;15(2):021301
20459223
IEEE Trans Med Imaging. 2009 Dec;28(12):1997-2006
19628454
J Opt Soc Am A Opt Image Sci Vis. 2006 Apr;23(4):878-88
16604770
Nat Biotechnol. 2003 Jul;21(7):803-6
12808463
Phys Med Biol. 2010 Apr 7;55(7):1917-34
20224160
Langmuir. 2010 Mar 16;26(6):3786-9
20166728
Radiology. 2000 Jul;216(1):279-83
10887262
J Opt Soc Am A Opt Image Sci Vis. 2009 Feb;26(2):443-55
19183699
Phys Med Biol. 2009 Feb 21;54(4):1035-46
19168938
Appl Opt. 2010 Mar 10;49(8):1219-33
20220877
Anal Chem. 1998 Jul 1;70(13):2651-9
21644785