PI4P and Rab inputs collaborate in myosin-V-dependent transport of secretory compartments in yeast.
Cell polarity involves transport of specific membranes and macromolecules at the right time to the right place. In budding yeast, secretory vesicles are transported by the myosin-V Myo2p to sites of cell growth. We show that phosphatidylinositol 4-phosphate (PI4P) is present in late secretory compartments and is critical for their association with, and transport by, Myo2p. Further, the trans-Golgi network Rab Ypt31/32p and secretory vesicle Rab Sec4p each bind directly, but distinctly, to Myo2p, and these interactions are also required for secretory compartment transport. Enhancing the interaction of Myo2p with PI4P bypasses the requirement for interaction with Ypt31/32p and Sec4p. Together with additional genetic data, the results indicate that Rab proteins and PI4P collaborate in the association of secretory compartments with Myo2p. Thus, we show that a coincidence detection mechanism coordinates inputs from PI4P and the appropriate Rab for secretory compartment transport.
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Felipe H
FH
Santiago-Tirado
Aster
A
Legesse-Miller
Daniel
D
Schott
Anthony
A
Bretscher
D000483:Alleles; D001692:Biological Transport; D002451:Cell Compartmentation; D016764:Cell Polarity; D006056:Golgi Apparatus / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D024701:Myosin Type V / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
D018129:Phosphatidylinositol Phosphates; D029701:Saccharomyces cerevisiae Proteins; C037178:phosphatidylinositol 4-phosphate; D024701:Myosin Type V; D020691:rab GTP-Binding Proteins
Mol Cell Biol. 2005 Apr;25(8):2910-23
15798181
J Biol Chem. 1999 May 7;274(19):12990-5
10224048
Cell. 1988 Jun 3;53(5):753-68
3131018
Dev Cell. 2006 May;10(5):587-600
16678774
J Biol Chem. 2004 Oct 22;279(43):44683-9
15271978
Mol Biol Cell. 2002 Feb;13(2):542-57
11854411
J Biol Chem. 2002 Dec 27;277(52):50415-21
12393859
J Cell Biol. 2003 Mar 17;160(6):887-97
12642614
Mol Biol Cell. 2004 Apr;15(4):2038-47
14767056
Curr Biol. 2002 Apr 30;12(9):695-704
12007412
Dev Cell. 2010 May 18;18(5):828-40
20493815
J Cell Biol. 2001 Apr 2;153(1):47-62
11285273
Mol Biol Cell. 2001 Jun;12(6):1843-57
11408590
J Biol Chem. 2002 Dec 6;277(49):47709-18
12361950
J Cell Biol. 2001 Dec 10;155(6):979-90
11733545
J Cell Biol. 1991 May;113(3):539-51
2016335
Mol Biol Cell. 2007 Aug;18(8):2828-37
17507647
Cell. 2009 Mar 20;136(6):1110-21
19303853
Curr Biol. 2008 Jul 8;18(13):987-91
18595704
Nature. 2003 Mar 6;422(6927):87-92
12594460
Dev Cell. 2002 May;2(5):593-605
12015967
Traffic. 2005 Feb;6(2):116-30
15634212
Nat Cell Biol. 1999 Dec;1(8):523-5
10587649
Curr Opin Cell Biol. 2000 Feb;12(1):63-71
10679357
Nucleic Acids Res. 1994 Dec 25;22(25):5767-8
7838736
Mol Biol Cell. 2000 Feb;11(2):691-702
10679024
EMBO J. 1998 Sep 1;17(17):4930-42
9724630
Mol Microbiol. 2008 Mar;67(5):1051-66
18221262
J Biol Chem. 1999 Nov 26;274(48):34294-300
10567405
Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11821-7
16882731
Mol Biol Cell. 1999 Apr;10(4):1001-17
10198053
EMBO J. 2004 Oct 1;23(19):3747-57
15372071
Mol Cell Biol. 2002 Nov;22(22):7744-57
12391144
Nature. 1992 Jan 9;355(6356):173-5
1729652
Mol Biol Cell. 2004 Aug;15(8):3567-79
15169871
Mol Biol Cell. 2001 Aug;12(8):2396-411
11514624
J Cell Biol. 2002 Jan 7;156(1):35-9
11781333
J Cell Biol. 1999 Nov 15;147(4):791-808
10562281
Science. 1993 Nov 26;262(5138):1444-8
8248783
Nature. 2006 Oct 12;443(7112):651-7
17035995
J Biol Chem. 1997 Oct 31;272(44):27671-7
9346907
J Cell Biol. 1997 May 5;137(3):563-80
9151665
Mol Biol Cell. 2008 Oct;19(10):4177-87
18653471
J Cell Biol. 2008 Apr 7;181(1):119-30
18391073
Nat Rev Mol Cell Biol. 2001 Feb;2(2):107-17
11252952
J Cell Biol. 2003 May 12;161(3):483-8
12743102
Methods Enzymol. 2001;329:91-9
11210577
Cell. 2002 May 3;109(3):347-58
12015984
Proc Natl Acad Sci U S A. 2010 May 4;107(18):8225-30
20404150
Mol Biol Cell. 2005 Feb;16(2):776-93
15574876
Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):3793-8
17360432
Nat Cell Biol. 2002 Apr;4(4):271-8
11887186
Nature. 2000 Aug 31;406(6799):1013-5
10984058
J Cell Biol. 1996 Dec;135(6 Pt 1):1535-49
8978821
J Cell Sci. 2008 Jun 15;121(Pt 12):1955-63
18525025
J Cell Biol. 2006 Jul 31;174(3):425-36
16864656