Description of Spongiibacter borealis sp. nov., isolated from Arctic seawater, and reclassification of Melitea salexigens Urios et al. 2008 as a later heterotypic synonym of Spongiibacter marinus Graeber et al. 2008 with emended descriptions of the genus Spongiibacter and Spongiibacter marinus.
A Gram-negative, rod-shaped and motile strain, designated CL-AS9(T), was isolated from polar seawater of the Arctic. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Spongiibacter, sharing 93.9% and 93.7% sequence similarities with the type strains of Spongiibacter tropicus CL-CB221(T) and Spongiibacter marinus HAL40b(T), respectively. Phylogenetic analyses revealed that strain CL-AS9(T) formed a separate branch that was distinct from a clade comprising Spongiibacter marinus HAL40b(T), Spongiibacter tropicus CL-CB221(T) and Melitea salexigens 5IX/A01/131(T). Cells of the strain grew optimally at 20-25 °C and pH 6.6-8.0 in the presence of 3-4% (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. The major quinone was ubiquinone 8. The major cellular fatty acids were C(16:1)ω7c and/or iso-C(15:0) 2-OH (23.1%), C(17:1)ω8c (22.1%) and C(18:1)ω7c (15.6%). The genomic DNA G+C content was 53.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, we propose the name Spongiibacter borealis sp. nov. with the type strain CL-AS9(T) (=KCCM 90094(T) =JCM 17304(T)) and the reclassification of Melitea salexigens as a later heterotypic synonym of Spongiibacter marinus. We also provide emended descriptions of the genus Spongiibacter and Spongiibacter marinus.
Microbial Oceanography Laboratory, School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Gwang Il
GI
Jang
Microbial Oceanography Laboratory, School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Chung Yeon
CY
Hwang
Division of Polar Biology and Ocean Sciences, Korea Polar Research Institute, Get-Pearl Tower, Songdo Technopark, 7-50 Songdo-dong, Yeonsu-gu, Incheon 406-840, Republic of Korea.
Han-Gu
HG
Choi
Division of Polar Biology and Ocean Sciences, Korea Polar Research Institute, Get-Pearl Tower, Songdo Technopark, 7-50 Songdo-dong, Yeonsu-gu, Incheon 406-840, Republic of Korea.
Sung-Ho
SH
Kang
Microbial Oceanography Laboratory, School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Byung Cheol
BC
Cho
D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S