[Influenza virus reproduction in the MDCK cells adapted to growth in serum-free Hybris-2 medium].
Whether the MDCK cell line might adapt to grow in serum-free Hybris-2 medium and influenza viruses might be reproduced in the adapted cells was studied. Seventeen passages using the Hybris-2 medium yielded cells adapted to growth in this medium (MDCK-BS). The reproduction of influenza A (H1N1 and H3N2) and B viruses versus the cells cultured in Eagle's medium was studied. The laboratory strain of influenza A/Aichi/1/68 (H3N2) and the strain B/Ohio/01/05 of influenza B in equal titers were shown to be reproduced in both control cells on Eagle's medium and MDCK-BS cells adapted to growth in the Hybris-2 medium. The reproduction of the strains A/Brisbane/10/07 (H3N2) and A/Solomon Islands/3/06 (H1N1) was less active in the MDCK cells. Each strain of influenza viruses displayed varying infective activities. The developed serum-free Hybris-2 medium may be used for cultivation of monolayer continuous MDCK cells and for their reproduction of influenza A and B viruses.
R Ia
RIa
Podcherniaeva
O V
OV
Baklanova
L A
LA
Glushchenko
E I
EI
Isaeva
V V
VV
Chestkov
V Iu
VIu
Tabakov
G R
GR
Mikhaĭlova
D000818:Animals; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004285:Dogs; D009980:Influenza A virus / Q000254:growth & development*; D009981:Influenza B virus / Q000254:growth & development*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D016895:Culture Media, Serum-Free