Spatial light interference microscopy (SLIM).
We present spatial light interference microscopy (SLIM) as a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry. SLIM combines two classic ideas in light imaging: Zernike's phase contrast microscopy, which renders high contrast intensity images of transparent specimens, and Gabor's holography, where the phase information from the object is recorded. Thus, SLIM reveals the intrinsic contrast of cell structures and, in addition, renders quantitative optical path-length maps across the sample. The resulting topographic accuracy is comparable to that of atomic force microscopy, while the acquisition speed is 1,000 times higher. We illustrate the novel insight into cell dynamics via SLIM by experiments on primary cell cultures from the rat brain. SLIM is implemented as an add-on module to an existing phase contrast microscope, which may prove instrumental in impacting the light microscopy field at a large scale.
Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science & Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Zhuo
Z
Wang
Larry
L
Millet
Mustafa
M
Mir
Huafeng
H
Ding
Sakulsuk
S
Unarunotai
John
J
Rogers
Martha U
MU
Gillette
Gabriel
G
Popescu
D000818:Animals; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation* / Q000379:methods*; D007089:Image Enhancement / Q000295:instrumentation*; D008029:Lighting / Q000295:instrumentation*; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D009474:Neurons / Q000166:cytology*; D051381:Rats
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