[Inhibitive effect of ursolic acid on the invasion and metastasis of ovarian carcinoma cells HO-8910PM].
OBJECTIVE
To study the effects of ursolic acid (UA) on the invasion and metastasis of ovarian carcinoma cell HO-8910PM and its underlying mechanism.


METHODS
MTT assay was performed to examine the effects of UA on the proliferation of ovarian carcinoma cells HO-8910PM in vitro. The effects of UA on the invasion and migration of HO-8910PM were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane. Gelatin zymography was performed to detect the activity of gelatinase in the HO-8910PM cells. The expressions of MMP-2 and MMP-9 in the HO-8910PM cells were measured by Western blot.


RESULTS
UA inhibited the proliferation of HO-8910PM cells in a time- and dose-dependent manner. There was a statistically significant difference in the invasion and migration of HO-8910PM cells between the UA treated cells and the controls (P < 0.01, P < 0.05). UA inhibited the activity of gelatinase of the treated cells.


CONCLUSIONS
UA downregulated the expressions of MMP-2 and MMP-9. UA inhibits the invasion and metastasis of HO-8910PM cells, probably through inhibiting the activity of gelatinase and the expressions of MMP-2 and MMP-9.
Affiliated Tumor Hospital of Harbin Medical University, Harbin 150081, China.
Li-bo
LB
Yu
Jing
J
Wang
Bao-zhang
BZ
Ma
Wen-zhou
WZ
Sun
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009362:Neoplasm Metastasis / Q000517:prevention & control; D010051:Ovarian Neoplasms / Q000473:pathology*; D014315:Triterpenes / Q000494:pharmacology*; D000097245:Ursolic Acid
D000970:Antineoplastic Agents; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D014315:Triterpenes; D000097245:Ursolic Acid; C522361:MMP2 protein, human