Solution structure of BTK-2, a novel hK(v)1.1 inhibiting scorpion toxin, from the eastern Indian scorpion Mesobuthus tamulus.
The three dimensional structure of a 32 residue three disulfide scorpion toxin, BTK-2, from the Indian red scorpion Mesobuthus tamulus has been determined using isotope edited solution NMR methods. Samples for structural and electrophysiological studies were prepared using recombinant DNA methods. Electrophysiological studies show that the peptide is active against hK(v)1.1 channels. The structure of BTK-2 was determined using 373 distance restraints from NOE data, 66 dihedral angle restraints from NOE, chemical shift and scalar coupling data, 6 constraints based on disulfide linkages and 8 constraints based on hydrogen bonds. The root mean square deviation (r.m.s.d) about the averaged co-ordinates of the backbone (N, C(α), C') and all heavy atoms are 0.81 ± 0.23Å and 1.51 ± 0.29Å respectively. The backbone dihedral angles (ϕ and ψ) for all residues occupy the favorable and allowed regions of the Ramachandran map. The three dimensional structure of BTK-2 is composed of three well defined secondary structural regions that constitute the α-β-β structural motif. Comparisons between the structure of BTK-2 and other closely related scorpion toxins pointed towards distinct differences in surface properties that provide insights into the structure-function relationships among this important class of voltage-gated potassium channel inhibiting peptides.
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India.
G Senthil
GS
Kumar
Sanjeev
S
Upadhyay
M K
MK
Mathew
Siddhartha P
SP
Sarma
D000595:Amino Acid Sequence; D000818:Animals; D004220:Disulfides / Q000378:metabolism; D055724:Electrophysiological Phenomena / Q000187:drug effects; D007194:India; D015640:Ion Channel Gating / Q000187:drug effects; D051662:Kv1.1 Potassium Channel / Q000037:antagonists & inhibitors*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000494:pharmacology; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification; D012605:Scorpions / Q000737:chemistry* / Q000187:drug effects; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D055672:Static Electricity; D013237:Stereoisomerism; D013816:Thermodynamics; D014981:Xenopus
C549471:BTK-2 toxin, Mesobuthus tamulus; D004220:Disulfides; C496946:KCNA1 protein, human; D010455:Peptides; D011994:Recombinant Proteins; D012604:Scorpion Venoms; D012996:Solutions; D051662:Kv1.1 Potassium Channel