Oxygenation of the portal vein by intraperitoneal administration of oxygenated perfluorochemical improves the engraftment and function of intraportally transplanted islets.
OBJECTIVE
One of the major obstacles for successful intraportal islet transplantation (IPIT) is early graft loss due to hypoxia. We therefore examined the effect of intraperitoneal oxygenated perfluorochemical (PFC) on oxygenation of the portal vein with respect to islet engraftment and function after IPIT in a rat model.


METHODS
First, we measured the oxygen tension and saturation in the portal vein of Lewis rats before and after intraperitoneal injection of oxygenated PFC. Second, blood glucose levels, glucose tolerance, and the number of surviving islets were measured after IPIT with oxygenated PFC (group 1), with PFC saturated by nitrogen (group 2), and without any PFC (control).


RESULTS
Both oxygen tension and saturation in the portal vein significantly increased after injection of oxygenated PFC. In IPIT, the functional success rate in group 1 was 83.3%, compared with 16.7% in group 2 and 16.7% in the control. On the 28th posttransplantation day, the number of engrafted islets in the liver in group 1 (12.8 [SD, 3.3]) was significantly higher than that in group 2 (4.7 [SD, 3.0]) and in the control group (6.5 [SD, 3.3]).


CONCLUSIONS
We clearly demonstrated the effect of intraperitoneal oxygenated PFC on oxygenation of the portal vein, resulting in better IPIT outcomes.
Department of Surgery, Kakogawa Municipal Hospital, Hyogo, Japan. sakai005@nifty.com
Tetsuya
T
Sakai
Shiri
S
Li
Yoshikazu
Y
Kuroda
Yasuki
Y
Tanioka
Yasuhiro
Y
Fujino
Yasuyuki
Y
Suzuki
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001802:Blood Substitutes / Q000008:administration & dosage*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery; D005466:Fluorocarbons / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D006085:Graft Survival; D007274:Injections, Intraperitoneal; D016381:Islets of Langerhans Transplantation / Q000379:methods* / Q000473:pathology / Q000502:physiology; D008099:Liver / Q000473:pathology; D008297:Male; D010100:Oxygen / Q000097:blood*; D011169:Portal Vein; D051381:Rats; D011917:Rats, Inbred Lew; D016042:Transplantation, Heterotopic; D014185:Transplantation, Isogeneic
D001786:Blood Glucose; D001802:Blood Substitutes; D005466:Fluorocarbons; C008805:perfluorodecalin; D010100:Oxygen