Practical implementation of log-scale active illumination microscopy.
Active illumination microscopy (AIM) is a method of redistributing dynamic range in a scanning microscope using real-time feedback to control illumination power on a sub-pixel time scale. We describe and demonstrate a fully integrated instrument that performs both feedback and image reconstruction. The image is reconstructed on a logarithmic scale to accommodate the dynamic range benefits of AIM in a single output channel. A theoretical and computational analysis of the influence of noise on active illumination feedback is presented, along with imaging examples illustrating the benefits of AIM. While AIM is applicable to any type of scanning microscope, we apply it here specifically to two-photon microscopy.
Boston University, Department of Biomedical Engineering 44 Cummington St., Boston, MA, 02215 kenchu@bu.edu http://biomicroscopy.bu.edu.
Kengyeh
K
K Chu
Daryl
D
Lim
Jerome
J
Mertz
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