Bacterial diversity in Solenopsis invicta and Solenopsis geminata ant colonies characterized by 16S amplicon 454 pyrosequencing.
Social insects harbor diverse assemblages of bacterial microbes, which may play a crucial role in the success or failure of biological invasions. The invasive fire ant Solenopsis invicta (Formicidae, Hymenoptera) is a model system for understanding the dynamics of invasive social insects and their biological control. However, little is known about microbes as biotic factors influencing the success or failure of ant invasions. This pilot study is the first attempt to characterize and compare microbial communities associated with the introduced S. invicta and the native Solenopsis geminata in the USA. Using 16S amplicon 454 pyrosequencing, bacterial communities of workers, brood, and soil from nest walls were compared between neighboring S. invicta and S. geminata colonies at Brackenridge Field Laboratory, Austin, Texas, with the aim of identifying potential pathogenic, commensal, or mutualistic microbial associates. Two samples of S. geminata workers showed high counts of Spiroplasma bacteria, a known pathogen or mutualist of other insects. A subsequent analysis using PCR and sequencing confirmed the presence of Spiroplasma in additional colonies of both Solenopsis species. Wolbachia was found in one alate sample of S. geminata, while one brood sample of S. invicta had a high count of Lactococcus. As expected, ant samples from both species showed much lower microbial diversity than the surrounding soil. Both ant species had similar overall bacterial diversities, although little overlap in specific microbes. To properly characterize a single bacterial community associated with a Solenopsis ant sample, rarefaction analyses indicate that it is necessary to obtain 5,000-10,000 sequences. Overall, 16S amplicon 454 pyrosequencing appears to be a cost-effective approach to screen whole microbial diversity associated with invasive ant species.
Section of Integrative Biology, University of Texas at Austin, 2401 Speedway Drive C0930, Austin, TX 78712, USA. heatherishak@gmail.com
Heather D
HD
Ishak
Rob
R
Plowes
Ruchira
R
Sen
Katrin
K
Kellner
Eli
E
Meyer
Dora A
DA
Estrada
Scot E
SE
Dowd
Ulrich G
UG
Mueller
D000818:Animals; D001000:Ants / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Proc Biol Sci. 2002 Oct 7;269(1504):2023-7
12396501
Curr Microbiol. 2008 Dec;57(6):575-9
18795363
Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21236-41
19948964
BMC Microbiol. 2009 Feb 06;9:29
19200360
Bioinformatics. 2001 Mar;17(3):282-3
11294794
BMC Bioinformatics. 2005 Apr 08;6:93
15819992
J Invertebr Pathol. 1977 Mar;29(2):192-200
850074
Front Biosci. 2006 Sep 01;11:2983-3002
16720370
Poult Sci. 2009 Feb;88(2):298-302
19151343
BMC Med Genomics. 2010 Sep 21;3:41
20854691
Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17805-10
19805175
Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1877-1886
11034499
Appl Environ Microbiol. 2007 Mar;73(5):1576-85
17220268
Mol Ecol. 2010 Jan;19(2):414-25
20002580
Curr Microbiol. 2009 May;58(5):478-82
19159973
Appl Environ Microbiol. 2009 Dec;75(23):7537-41
19801464
PLoS Biol. 2008 Dec 23;6(12):e2
19222304
Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6477-81
20231444
Ann Microbiol (Paris). 1984 Jan-Feb;135A(1):151-5
6712058
Science. 2010 Jul 9;329(5988):212-5
20616278
ISME J. 2010 Jan;4(1):17-27
19710709
Insect Mol Biol. 2006 Feb;15(1):89-93
16469072
Virology. 2007 Sep 1;365(2):457-63
17477949
Open Microbiol J. 2010 Aug 11;4:47-52
21339894
J Invertebr Pathol. 2005 Jul;89(3):203-9
16039667
BMC Microbiol. 2008 Mar 06;8:43
18325110
Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20339-43
19064909
Nucleic Acids Res. 2007;35(18):e120
17881377
Environ Entomol. 2009 Oct;38(5):1360-4
19825289
J Invertebr Pathol. 2008 May;98(1):20-33
18023455
Infect Immun. 2010 Apr;78(4):1509-19
20145094
Mol Ecol. 2009 Mar;18(6):1294-305
19226322
J Invertebr Pathol. 1998 Mar;71(2):175-6
9500936
Ecology. 2008 Jul;89(7):1824-36
18705370
Isr J Med Sci. 1984 Oct;20(10):995-7
6392181
Virology. 2004 Oct 10;328(1):151-7
15380366
Mol Biol Evol. 2009 Jul;26(7):1641-50
19377059
PLoS One. 2010 Aug 13;5(8):e12149
20730104