[Influence of ground beetle on steroid-induced BMSCs osteogenic differentiation].
OBJECTIVE
To investigate the pathogenesis of steroid-induced avascular necrosis of femoral head and the control mechanisms of Ground Beetle.


METHODS
The bone marrow-derived mesenchymal stem cells were cultured in vitro. The BMSCs in the 3rd generation were randomly divided into blank group, model group and Chinese medicine low, medium and high dose group. BMSCs of model group using high-dose steroid-induced in vitro were adipogenic differentiation to inhibit osteogenesis. Chinese medicine low, medium and high-dose groups at the same time were given the Ground Beetle intervention serum containing insects. The expression of osteocalcin, Alkaline phosphatase and Type I Collagen mRNA were detected and interfered 6 days later.


RESULTS
Serum containing Ground Beetle could reverse content of steroid-induced alkaline phosphatase of BMSCs, the expression of ostercalcin and Type I Collagen mRNA decreased.


CONCLUSIONS
The control mechanism of Ground Beetle on steroid-induced avascular necrosis is not only to improve the microcirculation, but also to inhibit steroid-induced osteogenic differentiation of BMSCs reduced.
Osteopaedics Institute of Fujian Academy of Integrated Traditional and Western Medicine, Fuzhou 350003, Fujian, China.
Shu-Qiang
SQ
Li
Tao
T
Yu
Zhen-Xi
ZX
Qi
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D001517:Coleoptera; D024042:Collagen Type I / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology*; D005271:Femur Head Necrosis / Q000139:chemically induced / Q000188:drug therapy; D008516:Medicine, Chinese Traditional; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D024042:Collagen Type I; D015675:Osteocalcin; D003907:Dexamethasone; D000469:Alkaline Phosphatase