Plasmodium falciparum field isolates use complement receptor 1 (CR1) as a receptor for invasion of erythrocytes.
A majority of Plasmodium falciparum strains invade erythrocytes through interactions with sialic acid (SA) on glycophorins. However, we recently reported that complement receptor 1 (CR1) is a SA-independent invasion receptor of many laboratory strains of P. falciparum. To determine the role of CR1 in erythrocyte invasion among P. falciparum field isolates, we tested eight isolates obtained from children in Kenya. All the parasites examined were capable of invading in a SA-independent manner, and invasion of neuraminidase-treated erythrocytes was nearly completely blocked by anti-CR1 and soluble CR1 (sCR1). In addition, anti-CR1 and sCR1 partially inhibited invasion of intact erythrocytes in a majority of isolates tested. Sequencing of the hypervariable region of P. falciparum AMA-1 showed considerable diversity among all the isolates. These data demonstrate that CR1 mediates SA-independent erythrocyte invasion in P. falciparum field isolates.
Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD, United States.
Gordon A
GA
Awandare
Carmenza
C
Spadafora
J Kathleen
JK
Moch
Sheetij
S
Dutta
J David
JD
Haynes
José A
JA
Stoute
D000595:Amino Acid Sequence; D000953:Antigens, Protozoan / Q000235:genetics; D002648:Child; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology; D005260:Female; D006790:Host-Parasite Interactions; D006801:Humans; D016778:Malaria, Falciparum / Q000378:metabolism* / Q000469:parasitology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010963:Plasmodium falciparum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics; D017463:Receptors, Complement 3b / Q000378:metabolism*; D016415:Sequence Alignment
D000953:Antigens, Protozoan; C509991:CR1 protein, human; D008565:Membrane Proteins; D015800:Protozoan Proteins; D017463:Receptors, Complement 3b; C090077:apical membrane antigen I, Plasmodium
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