Evaluation of rat alveolar bone response to Angelus MTA or experimental light-cured mineral trioxide aggregate using fluorochromes.
BACKGROUND
The aim of this study was to evaluate the rat alveolar bone response after the implantation of experimental light-cured mineral trioxide aggregate (MTA) or Angelus MTA (Angelus, Londrina, Paraná, Brazil) by histological and fluorescence analysis.


METHODS
Thirty Wistar Albino rats were divided into three groups. In the control group, empty polyethylene tubes were inserted into the rat alveolar sockets immediately after extraction. In the other groups, the tubes were filled with light-cured MTA or Angelus MTA. Five animals from each group were injected with calcein on day 7, alizarin on day 14, and oxytetracycline on day 21. On day 30, these animals were killed, and the right hemimaxillas were removed and histologically processed. Half of the maxillas were processed and stained with hematoxylin and eosin. The remaining maxillas were processed for fluorescence analysis and stained with Stevenel blue and alizarin red. New bone was histomorphometrically evaluated using a Merz grid.


RESULTS
The light-cured MTA presented a similar response when compared with Angelus MTA; it was characterized by a mild inflammatory response and complete bone healing. In the light-cured MTA group, the fluorescence areas were more evident at 21 days, showing an increase in bone formation. However, dystrophic mineralization was observed only with Angelus MTA.


CONCLUSIONS
It was concluded that both materials present a similar inflammatory response and bone healing, but dystrophic mineralization was observed only with Angelus MTA.
Department of Endodontics, University of Estadual Paulista, São Paulo, SP, Brazil. joao@foa.unesp.br
João Eduardo
JE
Gomes-Filho
Mariana Machado Teixeira
MM
de Moraes Costa
Luciano Tavares Angelo
LT
Cintra
Paulo Carvalho Tobias
PC
Duarte
Aline Satie
AS
Takamiya
Carolina Simonetti
CS
Lodi
Pedro Felício Estrada
PF
Bernabé
D017607:Aluminum Compounds / Q000494:pharmacology*; D000539:Alveolar Process / Q000187:drug effects*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D004338:Drug Combinations; D055115:Light-Curing of Dental Adhesives / Q000379:methods; D008297:Male; D016348:Osseointegration / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017640:Silicates / Q000494:pharmacology*; D020390:Tooth Socket / Q000187:drug effects*
D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate