Modification and evolution of Gluconobacter oxydans for enhanced growth and biotransformation capabilities at low glucose concentration.
Gluconobacter oxydans is widely used in several biotechnological applications, where sorbitol or mannitol is commonly used as carbon source at high concentration. In this study, a membrane-bound glucose dehydrogenase-deficient strain (GDHK) was constructed to eliminate growth problems on glucose caused by direct oxidation of glucose in the medium. To achieve improved growth properties for the GDHK strain on glucose, a laboratory adaptive evolution experiment was performed with glucose as the sole carbon source. Results indicated evident, albeit modest, improvements in cell growth after a 50-day (about 430 generations) experimental evolution on glucose. The maximum specific growth rate and biomass yield of the resulting GDHE50 strain were increased around 1.35- to 1.4-fold compared with those of the GDHK strain. Meanwhile, two types of biotransformation reactions using resting cells of G. oxydans were investigated. Significant elevations in biotransformation performance of the GHDE50 strain were observed in comparison with that of the wild-type strain. In addition, resting cells of the GDHE50 strain grown on a relatively low concentration of glucose (10 g/l) could catalyze the biotransformation of glycerol to dihydroxyacetone and ethylene glycol to glycolic acid as efficient as the wild-type G. oxydans cultured on higher concentration of sorbitol or other carbon sources. These results suggest very favorable prospects of using glucose to lower production cost in many important industrial biocatalysis and biotransformation processes.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Kun
K
Zhu
Leifang
L
Lu
Liujing
L
Wei
Dongzhi
D
Wei
Tadayuki
T
Imanaka
Qiang
Q
Hua
D018533:Biomass; D001711:Biotransformation; D002384:Catalysis; D003470:Culture Media / Q000378:metabolism; D004098:Dihydroxyacetone / Q000096:biosynthesis; D019855:Ethylene Glycol / Q000378:metabolism; D019143:Evolution, Molecular; D055786:Gene Knockout Techniques; D005798:Genes, Bacterial; D020600:Gluconobacter oxydans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D005947:Glucose / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D006016:Glycolates / Q000378:metabolism; D010084:Oxidation-Reduction; D010957:Plasmids; D017422:Sequence Analysis, DNA
D003470:Culture Media; D006016:Glycolates; C031149:glycolic acid; D042843:Glucose 1-Dehydrogenase; D019855:Ethylene Glycol; D005947:Glucose; D004098:Dihydroxyacetone; D005990:Glycerol
FEMS Microbiol Lett. 1997 Feb 15;147(2):181-8
9119191
J Mol Microbiol Biotechnol. 2009;16(1-2):6-13
18957858
Appl Environ Microbiol. 2007 Jul;73(14):4639-47
17513588
J Biol Chem. 1958 Dec;233(6):1295-8
13610830
Biotechnol Bioeng. 2006 Dec 5;95(5):992-1002
16807925
FEMS Microbiol Lett. 1999 Oct 15;179(2):501-6
10518757
FEMS Immunol Med Microbiol. 2003 Sep 22;38(2):113-6
13129645
J Ind Microbiol Biotechnol. 2009 Aug;36(8):1029-34
19434434
Appl Microbiol Biotechnol. 2003 Jul;62(1):92-8
12835926
Int J Food Microbiol. 2008 Jun 30;125(1):91-5
17976848
Biotechnol Bioeng. 2003 Oct 20;84(2):129-44
12966569
J Bacteriol. 2006 Nov;188(21):7668-76
16936032
Biotechnol Bioeng. 2003 Sep 20;83(6):687-94
12889033
Nat Biotechnol. 2005 Feb;23(2):195-200
15665824
J Mol Microbiol Biotechnol. 2009;16(1-2):69-80
18957863
Appl Environ Microbiol. 2010 Jul;76(13):4369-76
20453146
Biotechnol Bioeng. 2005 Sep 5;91(5):643-8
15962337
Appl Microbiol Biotechnol. 2003 Feb;60(6):643-53
12664142
Genome Biol. 2009;10(10):R118
19849850