Increased oxidative stress and iron overload in Jordanian β-thalassemic children.
β-Thalassemia (β-thal) is associated with abnormal synthesis of hemoglobin (Hb). Repeated blood transfusions in patients with β-thal major (β-TM) leads to an enhanced generation of reactive oxygen species (ROS), and subjects patients to peroxidative injury. We studied the antioxidant status and oxidative damage to children with β-thal in Jordan. Samples from 40 children with β-thal and 40 healthy controls were used. All children were under 13 years of age. Our results showed that plasma thiobarbituric acid reactive substances (TBARS) were elevated in β-thalassemic children compared to controls together with compensatory increase in superoxide dismutase (SOD) activity and decrease in catalase (CAT) activity. Elevated serum ferritin showed positive correlation with elevated liver enzyme levels except gamma glutamyl transferase (GGT), confirming liver involvement due to iron overload. Serum ferritin also showed a positive correlation with elevated TBARS and SOD, suggesting that iron overload is involved in the oxidative stress shown in cells.
Department of Biological Sciences and Biotechnology, The Hashemite University, Zarqa, Jordan. Abdallamy@mail.nih.gov
Maher Y
MY
Abdalla
Mohammad
M
Fawzi
Salem R
SR
Al-Maloul
Nasser
N
El-Banna
Reema F
RF
Tayyem
Iman M
IM
Ahmad
D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003676:Deferoxamine / Q000494:pharmacology; D057075:Enzyme Assays; D005260:Female; D005293:Ferritins / Q000032:analysis; D006801:Humans; D007501:Iron / Q000097:blood*; D019190:Iron Overload / Q000097:blood* / Q000188:drug therapy; D007597:Jordan; D008099:Liver / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D065227:Transfusion Reaction; D017086:beta-Thalassemia / Q000097:blood* / Q000188:drug therapy
D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D005293:Ferritins; D007501:Iron; D002374:Catalase; D013482:Superoxide Dismutase; D003676:Deferoxamine