Life cycle analysis of the model organism Rhodopirellula baltica SH 1(T) by transcriptome studies.
The marine organism Rhodopirellula baltica is a representative of the globally distributed phylum Planctomycetes whose members exhibit an intriguing lifestyle and cell morphology. The analysis of R. baltica's genome has revealed many biotechnologically promising features including a set of unique sulfatases and C1-metabolism genes. Salt resistance and the potential for adhesion in the adult phase of the cell cycle were observed during cultivation. To promote the understanding of this model organism and to specify the functions of potentially useful genes, gene expression throughout a growth curve was monitored using a whole genome microarray approach. Transcriptional profiling suggests that a large number of hypothetical proteins are active within the cell cycle and in the formation of the different cell morphologies. Numerous genes with potential biotechnological applications were found to be differentially regulated, revealing further characteristics of their functions and regulation mechanisms. More specifically, the experiments shed light on the expression patterns of genes belonging to the organism's general stress response, those involved in the reorganization of its genome and those effecting morphological changes. These transcriptomic results contribute to a better understanding of thus far unknown molecular elements of cell biology. Further, they pave the way for the biotechnological exploitation of R. baltica's distinctive metabolic features as a step towards sourcing the phylum Planctomycetes at large.
Max Planck Institute for Marine Microbiology, Microbial Genomics Group, Celsiusstr. 1, 28359 Bremen, Germany.
Patricia
P
Wecker
Christine
C
Klockow
Margarete
M
Schüler
Jérôme
J
Dabin
Gurvan
G
Michel
Frank O
FO
Glöckner
D001419:Bacteria / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D008954:Models, Biological
D001426:Bacterial Proteins
Genome Biol. 2007;8(4):R65
17456242
Am J Psychiatry. 1977 Apr;134(4):366-70
320887
Annu Rev Plant Biol. 2003;54:207-33
14502990
Proteomics. 2008 Apr;8(8):1608-23
18340632
Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8
18838391
Biotechniques. 2003 Feb;34(2):374-8
12613259
Curr Opin Biotechnol. 2006 Jun;17(3):241-9
16704931
Proteomics. 2005 Sep;5(14):3654-71
16127728
BMC Bioinformatics. 2008 Apr 01;9:177
18380896
Arch Microbiol. 2001 Jun;175(6):413-29
11491082
Nucleic Acids Res. 2003 Apr 15;31(8):2187-95
12682369
Mol Biol Evol. 2004 Jul;21(7):1234-41
15014146
Biochem Soc Trans. 2006 Apr;34(Pt 2):296-300
16545098
Proteomics. 2005 Sep;5(14):3672-83
16127727
ISME J. 2007 Sep;1(5):419-35
18043661
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Mar 1;64(Pt 3):224-7
18323615
Appl Environ Microbiol. 2008 Jul;74(13):4091-100
18469119
BMC Genomics. 2008 Oct 16;9:488
18925949
BMC Genomics. 2009 Sep 02;10:410
19725962
Trends Biotechnol. 2007 Nov;25(11):514-21
17935805
Appl Microbiol Biotechnol. 2006 Jun;71(1):23-33
16550377
Proteomics. 2002 Jun;2(6):649-55
12112844
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1567-1580
15388712
J Basic Microbiol. 2007 Apr;47(2):103-17
17440912
J Bacteriol. 1997 May;179(10):3324-30
9150231
Environ Microbiol. 2005 Aug;7(8):1074-84
16011746
J Bacteriol. 2006 Dec;188(23):8044-53
16980445
Science. 2005 Aug 5;309(5736):936-8
16081736
Chem Rev. 2002 Feb;102(2):439-69
11841250
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8298-303
12835416