[Isolation and identification of a polyhydroxyalkanoate producing strain].
OBJECTIVE
We screened and isolated polyhydroxyalkanoate producing bacteria.


METHODS
The strains were isolated from sludge from a beer brewery and screened by Sudan black B staining method. The isolated strains were identified according to their morphological features, physiological and biochemical analysis as well as 16S rRNA gene sequence analysis. The product extracted with hot chloroform from the isolated strain HG-B-1 was confirmed by Fourier transform infrared spectra.


RESULTS
We isolated a bacterium, HG-B-1, from sludge collected from a beer brewery in Guangdong province, China. The yield of polyhydroxyalkanoates was 23.4% (w/w) based on dried weight of the bacterium cells when HG-B-1 grew in a medium containing saccharose. We analyzed 16S rRNA nucleotide sequence, and ascertained the phylogenetic position of the strain.


CONCLUSIONS
Strain HG-B-1 with PHAs biosynthesis ability was identified as Stenotrophomonas maltophlia.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Xueyun
X
Li
Shiyu
S
Fu
Jichuan
J
Yu
Kai
K
Fu
Yuancai
Y
Chen
Ruizhe
R
Zhang
Yunsi
Y
Liu
D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D020588:Stenotrophomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D004269:DNA, Bacterial; D054813:Polyhydroxyalkanoates; D012336:RNA, Ribosomal, 16S; D012722:Sewage