Depth-resolved measurement of mucosal microvascular blood content using  low-coherence enhanced backscattering spectroscopy.
Low-coherence enhanced backscattering (LEBS) spectroscopy is a light scattering technique which uses partial spatial coherence broadband illumination to interrogate the optical properties at sub-diffusion length scales. In this work, we present a post-processing technique which isolates the hemoglobin concentration at different depths within a sample using a single spectroscopic LEBS measurement with a fixed spatial coherence of illumination. We verify the method with scattering (spectralon reflectance standard and polystyrene microspheres) and absorbing (hemoglobin) phantoms. We then demonstrate the relevance of this method for quantifying hemoglobin content as a function of depth within biological tissue using the azoxymethane treated animal model of colorectal cancer.
Andrew J
AJ
Radosevich
Vladimir M
VM
Turzhitsky
Nikhil N
NN
Mutyal
Jeremy D
JD
Rogers
Valentina
V
Stoyneva
Ashish Kumar
AK
Tiwari
Mart
M
De La Cruz
Dhananjay P
DP
Kunte
Ramesh K
RK
Wali
Hemant K
HK
Roy
Vadim
V
Backman
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