Label-free detection of telomerase activity in HeLa cells using electrochemical impedance spectroscopy.
A simple assay based on electrochemical impedance spectroscopy (EIS) for detection of telomerase activity is developed, and it is demonstrated that the label-free EIS method is capable of detecting the telomerase activity in HeLa cells with a detection limit of 1000 HeLa cells without using any amplification technique.
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, 350002, China.
Weiqiang
W
Yang
Xi
X
Zhu
Qida
Q
Liu
Zhenyu
Z
Lin
Bin
B
Qiu
Guonan
G
Chen
D001483:Base Sequence; D058266:Dielectric Spectroscopy / Q000379:methods*; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000378:metabolism*
D005292:Ferricyanides; C007931:hexacyanoferrate III; D019098:Telomerase