Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging.
Laser Speckle Contrast Imaging (LSCI) has become a widely used technique to image cerebral blood flow in vivo. However, the quantitative accuracy of blood flow changes measured through the thin skull has not been investigated thoroughly. We recently developed a new Multi Exposure Speckle Imaging (MESI) technique to image blood flow while accounting for the effect of scattering from static tissue elements. In this paper we present the first in vivo demonstration of the MESI technique. The MESI technique was used to image the blood flow changes in a mouse cortex following photothrombotic occlusion of the middle cerebral artery. The Multi Exposure Speckle Imaging technique was found to accurately estimate flow changes due to ischemia in mice brains in vivo. These estimates of these flow changes were found to be unaffected by scattering from thinned skull.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712 ashwinbharadwaj@mail.utexas.edu.
Ashwin B
AB
Parthasarathy
S M Shams
SM
Kazmi
Andrew K
AK
Dunn
Eur J Neurosci. 2004 Nov;20(10):2664-70
15548209
IEEE Trans Med Imaging. 2008 Dec;27(12):1728-38
19033089
Appl Opt. 1981 Jun 15;20(12):2097-107
20332893
Opt Lett. 2006 Dec 1;31(23):3465-7
17099751
J Cereb Blood Flow Metab. 2001 Mar;21(3):195-201
11295873
Opt Express. 2009 Mar 2;17(5):3884-902
19259230
J Opt Soc Am A Opt Image Sci Vis. 2008 Aug;25(8):2088-94
18677371
Brain. 2007 Sep;130(Pt 9):2310-9
17638859
Opt Express. 2009 Aug 3;17(16):13904-17
19654798
J Cereb Blood Flow Metab. 2004 May;24(5):518-25
15129183
Phys Med Biol. 2001 Aug;46(8):2053-65
11512610
Opt Lett. 2008 Dec 15;33(24):2886-8
19079481
J Cereb Blood Flow Metab. 2006 Aug;26(8):1018-30
16340958
Med Eng Phys. 1994 Mar;16(2):105-11
8205359
Stroke. 2004 Sep;35(9):2177-82
15256680
Opt Express. 2008 Feb 4;16(3):1975-89
18542277
Microvasc Res. 2004 Sep;68(2):143-6
15313124
Physiol Meas. 2001 Nov;22(4):R35-66
11761081
Surg Neurol. 2007 Jun;67(6):620-5; discussion 625
17512331
Opt Lett. 2006 Jun 15;31(12):1824-6
16729083
J Biomed Opt. 2010 Jan-Feb;15(1):011109
20210435
Ann Neurol. 1985 May;17(5):497-504
4004172
J Cereb Blood Flow Metab. 2004 Jul;24(7):744-55
15241182
Am J Ophthalmol. 2000 Nov;130(5):606-10
11078839
Appl Opt. 2005 Apr 1;44(10):1823-30
15813518