Expression of a Brassica napus heme oxygenase confers plant tolerance to mercury toxicity.
Plant heme oxygenases (HOs) regulate biosynthesis of phytochrome which accounts for photo-acceptance and -morphogenesis. Recent studies have demonstrated that plant HOs also regulate many other physiological processes including response to environmental stimuli. To elucidate the mechanism by which HOs regulate plant adaptation to heavy metal exposure, three novel HOs genes were isolated from rapeseed (Brassica napus) and their expression patterns were analysed. Alignment of deduced protein sequences revealed that the three BnHOs share high identity with their corresponding orthologos (AtHO1-3) from Arabidopsis. To investigate whether the BnHO regulates plant tolerance to Hg toxicity, we constructed B. napus transgenic plants overexpressing BnHO-1. Under Hg stress, the transgenic plants had 1.41-1.59 folds higher biomass than the untransformants. However, overexpression of BnHO-1 resulted in less accumulation of Hg in some lines of transformants than in untransformants. The transgenic plants show lower abundance of reactive oxygen species and attenuated oxidative injury compared with the untransgenic plants. We cloned the promoter sequences of BnHO-1 from B. napus. Analysis revealed that the 1119 bp fragment contains a conserved Cd responsive element (CdRE) and others responding to multiple environmental stimuli. Transient expression in tobacco leaves showed differential responses to heavy metals (Zn, Cu, Pb, Hg and Cd).
Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Qi
Q
Shen
Ming
M
Jiang
Hua
H
Li
Li Ling
LL
Che
Zhi Min
ZM
Yang
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D018533:Biomass; D029688:Brassica napus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D008628:Mercury / Q000633:toxicity*; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011401:Promoter Regions, Genetic; D018749:RNA, Plant / Q000235:genetics; D017382:Reactive Oxygen Species / Q000032:analysis; D016415:Sequence Alignment; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
D010940:Plant Proteins; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D006419:Heme Oxygenase (Decyclizing); D008628:Mercury