3,4,5,6-Tetrahydroxyxanthone preserves intercellular communication by reduction of the endogenous nitric oxide synthase inhibitor level.
To observe the direct effects of 3,4,5,6-tetrahydroxyxanthone on connexin43 (Cx43) expression in cultured endothelial cells, cells were treated with lysophosphatidylcholine (LPC, 10 mg/l) for 24 h in the presence or absence of different concentrations of 3,4,5,6-tetrahydroxyxanthone (1, 3, or 10 μmol l(- 1)). The reactive oxygen species (ROS) production, cell viability, asymmetric dimethylarginine (ADMA) levels, and Cx43 expression were detected. 3,4,5,6-Tetrahydroxyxanthone significantly inhibited the increase in ROS production and ADMA level, increased cell viability and up-regulated Cx43 mRNA and protein expression induced by LPC. 3,4,5,6-Tetrahydroxyxanthone has protective effect in LPC-induced atherosclerotic lesions, which is at least partly related to the reduction of ADMA level and downregulation of Cx43 expression.
Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, China.
Su-Jie
SJ
Jia
Bi-Kui
BK
Zhang
Yong-Quan
YQ
Lai
Han-Wu
HW
Deng
Yuan-Jian
YJ
Li
D001120:Arginine / Q000031:analogs & derivatives / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D015394:Molecular Structure; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000037:antagonists & inhibitors*; D017382:Reactive Oxygen Species / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology*
C518384:3,4,5,6-tetrahydroxyxanthone; D018031:Connexin 43; D008244:Lysophosphatidylcholines; D017382:Reactive Oxygen Species; D044004:Xanthones; C018524:N,N-dimethylarginine; D001120:Arginine; D019001:Nitric Oxide Synthase