Glycan analysis by modern instrumental methods.
The oligosaccharides attached to proteins or lipids are among the most challenging analytical tasks due to their complexity and variety. Knowing the genes and enzymes responsible for their biosynthesis, a large but not unlimited number of different structures and isomers of such glycans can be imagined. Understanding of the biological role of structural variations requires the ability to unambiguously determine the identity and quantity of all glycan species. Here, we examine, which analytical strategies - with a certain high-throughput potential - may come near this ideal. After an expose of the relevant techniques, we try to depict how analytical raw data are translated into structural assignments using retention times, mass and fragment spectra. A method's ability to discriminate between the many conceivable isomeric structures together with the time, effort and sample amount needed for that purpose is suggested as a criterion for the comparative assessment of approaches and their evolutionary stages.
Department of Chemistry, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
Martin
M
Pabst
Friedrich
F
Altmann
D000818:Animals; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary; D054794:Glycomics / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009844:Oligosaccharides; D040901:Proteomics / Q000379:methods*
D006023:Glycoproteins; D009844:Oligosaccharides