[Study on invasion and metastasis-associated genes of lung cancer related with NM23-H1 gene].
OBJECTIVE
To study on invasion and metastasis-associated genes of lung cancer related with NM23-H1 gene.


METHODS
Human gene expression chip based on the subtracted cDNA libraries was constructed. After microarray hybridization, clones sequencing, sequence homology search, the information of differently expressed genes in human large cell lung cancer cell line of L9981 and L9981-nm23-H1 were obtained and then further confirmed by real-time quantitative PCR.


RESULTS
Gene expression profiling chips of differently expressed genes in human large cell lung cancer cell line L9981 and L9981-nm23-H1 were successfully constructed. After microarray hybridization, sequence homology search, 19 differentially expressed genes were observed. After real-time quantitative PCR evaluation, we found that the mRNA of 8 genes including PSMA7, SBDS, ODC1, YARS, CSDA, PTP4A1, SHPRH and TOMM7 was up-regulated in the cell line of L9981 after transfected with NM23-H1 gene, whereas the mRNA of PKM2 and GMNN was down-regulated.


CONCLUSIONS
NM23-H1 gene may be the upstream regulator of metastasis-associated genes, which can regulate the downstream genes to achieve a series of lung cancer metastatic potential.
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Chun-ji
CJ
Cai
Su-juan
SJ
Ye
Wen
W
Zhu
Li-ya
LY
Sun
Hai-su
HS
Wan
Zhi-hua
ZH
Feng
Li
L
Ma
Lu
L
Li
D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D054778:NM23 Nucleoside Diphosphate Kinases / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D059467:Transcriptome
D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human