Developmental competence of antral follicles and their oocytes after gonadotrophin treatment of sows with gene polymorphisms for leptin and melanocortin receptors (Iberian pig).
OBJECTIVE
To evaluate possible differences in follicle and oocyte developmental competence after gonadotrophin treatment in sows of obese and lean genotypes.


METHODS
Follicle dynamics, ovulation rate and oocyte developmental competence to embryo were compared between females, of obese (n = 7) and lean genotypes (n = 10), treated with 1,250 I.U. of eCG and 500 I.U. of hCG.


RESULTS
The obese genotype showed lower numbers of follicles growing to preovulatory stages (12.4 ± 1.8 vs 18.6 ± 1.0, P < 0.05), of corpora lutea (16.0 ± 0.9 vs 23.5 ± 0.9, P < 0.05), and of recovered oocytes/embryos (8.0 ± 1.3 vs 12.9 ± 0.9, P < 0.05). Thereafter, embryo viability rates also decreased when compared to lean genotypes (62.5 vs 77.6%, P < 0.05).


CONCLUSIONS
To our knowledge, this is the first study analyzing the effect of obese genotypes on the ovarian response to exogenous gonadotrophins in a non-rodent animal model, the pig. A lower efficiency of gonadotrophin treatments for stimulation of follicle development and induction of ovulation was observed.
ACOMPOR PIGS, Segovia, Spain.
Jorge
J
Muñoz-Frutos
Teresa
T
Encinas
Pilar
P
Pallares
Laura
L
Torres-Rovira
Pedro
P
Gonzalez-Añover
Emilio
E
Gomez-Izquierdo
Raul
R
Sanchez-Sanchez
Antonio
A
Gonzalez-Bulnes
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000378:metabolism; D003433:Crosses, Genetic; D005260:Female; D005838:Genotype; D020738:Leptin / Q000235:genetics*; D009865:Oocytes / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D011110:Polymorphism, Genetic; D044101:Receptors, Melanocortin / Q000235:genetics*; D013552:Swine; D014463:Ultrasonography
D006063:Chorionic Gonadotropin; D020738:Leptin; D044101:Receptors, Melanocortin
Reprod Biol. 2003 Nov;3(3):227-39
14688823
J Reprod Fertil Suppl. 1981;30:119-33
6300383
Theriogenology. 2011 Jan 1;75(1):34-41
20833418
Reprod Biomed Online. 2006 May;12(5):562-8
16790099
Annu Rev Physiol. 2008;70:537-56
17937601
Endocr Rev. 2010 Aug;31(4):506-43
20190196
Hum Reprod Update. 2000 May-Jun;6(3):290-300
10874574
J Reprod Dev. 2008 Dec;54(6):431-8
18787307
J Reprod Fertil Suppl. 1990;40:163-77
2192035
Meat Sci. 2006 May;73(1):144-50
22062063
Mamm Genome. 2010 Dec;21(11-12):583-91
21128076
J Reprod Dev. 2005 Aug;51(4):427-32
15947458
Meat Sci. 1998;49S1:S17-27
22060709
Cell. 1995 Dec 29;83(7):1263-71
8548812
Theriogenology. 2000 Mar 15;53(5):1063-70
10798484
Curr Biol. 2010 Oct 12;20(19):1729-34
20869245
Theriogenology. 2009 Jun;71(9):1433-9
19285717
Meat Sci. 2006 May;73(1):42-7
22062052
Gen Comp Endocrinol. 2011 Jan 1;170(1):200-6
20951700
Endocrinology. 2010 Aug;151(8):4039-46
20573727
Obesity (Silver Spring). 2007 Jan;15(1):40-9
17228030
Cell. 1996 Feb 9;84(3):491-5
8608603
Mol Endocrinol. 2004 Jan;18(1):150-61
14525952
Reprod Biomed Online. 2006 May;12(5):542-51
16790096
Biol Reprod. 2003 Mar;68(3):789-96
12604627
J Anim Sci. 1988 May;66(5):1230-5
3397350
J Reprod Fertil Suppl. 1999;54:449-60
10692875
J Clin Endocrinol Metab. 2001 Sep;86(9):4434-9
11549688
Endocrinology. 1997 Nov;138(11):5055-8
9348239
J Anim Sci. 2009 Feb;87(2):459-68
18952727
Ann N Y Acad Sci. 2008 Apr;1127:140-6
18443342
J Reprod Dev. 2005 Feb;51(1):109-15
15750302
Obes Rev. 2004 Feb;5(1):3-12
14969502
Genet Res. 2005 Feb;85(1):57-67
16089036
Scand J Clin Lab Invest. 2000 Dec;60(8):649-55
11218147
Hum Reprod. 2004 Nov;19(11):2523-8
15319380
Nat Clin Pract Endocrinol Metab. 2008 Oct;4(10):569-77
18779842
Theriogenology. 2010 May;73(8):1088-95
20172601
Fertil Steril. 2010 Feb;93(2):447-54
19171335
Menopause. 2007 Sep-Oct;14(5):835-40
17667144
J Reprod Fertil Suppl. 1993;48:61-73
8145215
Reprod Biol. 2006;6 Suppl 1:59-87
16967090
Reprod Biol Endocrinol. 2009 Mar 24;7:24
19309531
J Reprod Fertil Suppl. 1985;33:1-19
3003359
J Anim Sci. 2005 Jan;83(1):130-5
15583052
Nature. 1996 Feb 15;379(6566):632-5
8628397
Hum Genet. 2001 Mar;108(3):233-6
11354636
J Clin Endocrinol Metab. 1996 Sep;81(9):3424-7
8784109
Reprod Fertil Dev. 2009;21(2):313-22
19210922
Science. 1996 Feb 16;271(5251):994-6
8584938
Nature. 1998 Mar 26;392(6674):398-401
9537324
Endocrinol Metab Clin North Am. 2008 Sep;37(3):733-51, x
18775361
Anim Reprod Sci. 2010 Jun;119(3-4):329-34
20197222
Br J Nutr. 2002 Jul;88(1):39-49
12117426
Am J Epidemiol. 2005 Jul 15;162(2):101-14
15972940
J Endocrinol. 1997 Aug;154(2):285-92
9291839
J Reprod Fertil. 1983 Sep;69(1):343-52
6411913
Fertil Steril. 2007 Aug;88(2):446-51
17418840