Magnetic glyconanoparticles as a versatile platform for selective immunolabeling and imaging of cells.
A versatile nanoplatform based on magnetic glyconanoparticles (glyco-ferrites) to attach well-oriented antibodies is described. An efficient ligand exchange process has been used to prepare water-soluble 6-nm-sized core-shell Fe(3)O(4)@Au nanoparticles bearing amphiphilic carbohydrates and aliphatic ethylene glycol chains ended in a carboxyl group. The covalent immobilization through the carboxyl group of an Fc receptor (protein G) enables successful well-oriented capture of immunoglobulins G onto the magnetic glyconanoparticle. A thorough characterization of structure and biofunctionality of the constructs is carried out by different techniques. The selective immunolabeling of cells by the antibody-magnetic glyconanoparticle conjugates is demonstrated by magnetic resonance imaging (MRI), as well as by fluorescence techniques.
Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CICbiomaGUNE and CIBER-BBN, P° de Miramón 182, 20009 San Sebastian, Spain.
Isabel
I
García
Juan
J
Gallo
Nuria
N
Genicio
Daniel
D
Padro
Soledad
S
Penadés
D002241:Carbohydrates / Q000737:chemistry*; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002241:Carbohydrates; D005290:Ferric Compounds; D006001:Glycoconjugates; D009942:Organometallic Compounds; C001215:ferrite; D006046:Gold