Display of Bombyx mori nucleopolyhedrovirus GP64 on the Bacillus subtilis spore coat.
To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, pJS700-GP64 was transformed into B. subtilis 168 (trp(-)) competent cells, an amylase (amyE) inactivated mutant was selected, and was confirmed to be a double cross-over integrant, cotC-Gp64 fragment of which was integrated into B. subtilis chromosome. Gp64 was expressed on the spore surface and recognized by Gp64-specific antibody. Results of B. mori when challenged with BmNPV indicated that B. mori vaccinated with the recombinant spores possessed resistance to the invasion of BmNPV at some degree.
Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, China.
Guohui
G
Li
Qi
Q
Tang
Huiqing
H
Chen
Qin
Q
Yao
Degang
D
Ning
Keping
K
Chen
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D012831:Bombyx / Q000276:immunology / Q000382:microbiology / Q000821:virology*; D015870:Gene Expression; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D013171:Spores, Bacterial / Q000235:genetics* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D014759:Viral Envelope Proteins
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