[Effects of enalapril on IL-1beta, IL-6 expression in rat lung exposure to acrolein].
OBJECTIVE
To investigate the effects of enalapril on the expressions of IL-1beta and IL-6 in the lung of rats treated with acrolein inhalation.


METHODS
Inflammatory lung injury was induced by acrolein inhalation in rats. The rats were divided into natrium solution (NS) group, acrolein group, enalapril and acroclein (EA) group, enalapril and NS (EN) group. Lungs were harvested from the rats in each group at 1 week, 3 weeks, 6 weeks after the treatment of natrium solution, acrolein inhalation and/or enalapril. The mRNA and protein expressions of IL-1beta and IL-6 in the lung tissues were measured by RT-PCR, in situ hybridization, immunohistochemistry and Western blot.


RESULTS
Increased immunostaining, protein level and mRNA expression of IL-1beta and IL-6 were found in rat lung at 3 weeks and reached to the peak at 6 weeks post exposure to acrolein. The administration of enalapril resulted in a significant downregulation of IL-1beta and IL-6 in both protein and gene level, accompany with the decrease of inflammation.


CONCLUSIONS
Enalapril, as a ACE inhibitor, could protect the airway from inflammation injury in acrolein-treated rats via the down-regulation of IL-1beta and IL-6 expression.
Department of Respiration, the First Affiliated Hospital of Yunnan Province, Kunming Medical College, Kunming 650000, China.
Sheng-lan
SL
Wang
Bing
B
Yuan
Qi-qin
QQ
Dan
Xiao-yan
XY
Yang
Bu-liang
BL
Meng
Yun-hui
YH
Zhang
D000171:Acrolein / Q000633:toxicity*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D004656:Enalapril / Q000494:pharmacology / Q000627:therapeutic use*; D004781:Environmental Exposure / Q000009:adverse effects; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D008171:Lung Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000806:Angiotensin-Converting Enzyme Inhibitors; D053583:Interleukin-1beta; D015850:Interleukin-6; D004656:Enalapril; D000171:Acrolein