Magnetic loading of tyrosinase-Fe3O4/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing.
A new protocol is proposed for magnetic loading and sensitive electrochemical detection of phenol via the tyrosinase cross-linked mesoporous magnetic core/shell microspheres. The mesoporous magnetic microspheres, characterized by transmission electron microscopy, N(2) adsorption/desorption isotherms, and magnetic curve displays high capacity for enzyme immobilization and strong magnetism to adhere to the magnetic electrode surface without any additional adhesive reagent. The biosensor exhibits a wide linear response to phenol ranging from 1.0×10(-9) to 1.0×10(-5) M, a high sensitivity of 78 μA mM(-1), a low detection limit of 1 nM, and a fast response rate (less than 5s). The proposed method is simple, rapid, inexpensive and convenient in electrode renewal, which is recommended as a promising experimental platform for wider applications in biosensing.
School of Chemistry, Dalian University of Technology, Dalian 116023, PR China. wushuo@dlut.edu.cn
Shuo
S
Wu
Hainan
H
Wang
Shengyang
S
Tao
Chan
C
Wang
Lihui
L
Zhang
Zhiguang
Z
Liu
Changgong
C
Meng
D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D008280:Magnetics; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D019800:Phenol / Q000032:analysis*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry
D004800:Enzymes, Immobilized; D019800:Phenol; D012822:Silicon Dioxide; D014442:Monophenol Monooxygenase; D052203:Ferrosoferric Oxide