[Determination of glyphosate and aminomethylphosphonic acid in rice using liquid chromatography-tandem mass spectrometry].
A method was developed for the determination of glyphosate (GLY) and aminomethylphosphonic acid (AMPA) in rice using liquid chromatography tandem mass spectrometry (LC-MS/MS). The sample was extracted with water followed by a simple cleanup with a C18 solid phase extraction (SPE) cartridge, and then GLY and AMPA were derivatized using 9-fluorenylmethoxycarbonyl (FMOC-Cl) in borate buffer. The derivatives of GLY and AMPA were separated on a C18 column with gradient elution with the mobile phase of acetonitrile and 5 mmol/L ammonium acetate (pH 9), and finally detected with negative ion electrospray ionization-mass spectrometry (ESI-MS) in multiple reaction monitoring (MRM) mode. The results showed that the linearities of GLY and AMPA were in the concentration range of 0.000 50 to 1.0 mg/L with the correlation coefficients of 0.999 7 and 0.999 9, respectively. The mean spiked recoveries of GLY and AMPA at 3 spiked levels ranged from 72.5% to 113.6% with the relative standard deviations (RSD, n = 5) of 3.8% - 16.2%. The limits of detection were 2.0 and 3.0 microg/kg for GLY and AMPA, respectively. This method is rapid, sensitive, and suitable for simultaneous determination of GLY and AMPA in rice.
Rice Product Quality Inspection and Supervision Center of Ministry of Agriculture, China National Rice Research Institute, Hangzhou 310006, China.
Zhaoyun
Z
Cao
Renxiang
R
Mou
Mingxue
M
Chen
D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D006540:Herbicides / Q000032:analysis; D007555:Isoxazoles; D063065:Organophosphonates / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013777:Tetrazoles; D000097797:Glyphosate
D005998:Glycine; D006540:Herbicides; D007555:Isoxazoles; D063065:Organophosphonates; D010573:Pesticide Residues; D013777:Tetrazoles; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid