Role of bacterioferritin comigratory protein and glutathione peroxidase-reductase system in promoting bentazone tolerance in a mutant of Synechococcus elongatus PCC7942.
In this article, we describe the modifications in the antioxidant system of Synechococcus elongatus PCC7942 mutant Mu2 capable of growing at five times higher concentration of bentazone than wild type. Nevertheless, in both the strains, bentazone almost identically induced light-dependent H(2)O(2) production and its extracellular release. However unlike the wild type, peroxide produced upon prolong bentazone incubation was immediately degraded in Mu2. Consequently, the lipid peroxidation activity was also kept low. With prolong incubation of bentazone the mutant displayed a steady increase in glutathione peroxidase-reductase enzyme activities and reduced glutathione content, respectively, by 60% and 130%, favoring an efficient detoxification of bentazone-produced H(2)O(2). Catalase-peroxidase and glutathione S-transferase, though present, remained ineffective in rendering bentazone tolerance. In-gel assays of glutathione S-transferase and glutathione reductase revealed presence of between four and five oligomeric states with mobility shifts. One oligomeric form each enzyme in wild-type strain disappeared upon bentazone treatment. Upon two-dimensional electrophoresis and MALDI-TOF/TOF, a bacterioferritin comigratory protein (peroxiredoxin Q) was found to be already highly expressed in Mu2; whereas in wild type, its level increased only upon bentazone exposure. The bcp transcript pool in WT was relatively low but increased with bentazone, whereas Mu2 exhibited high bcp mRNA even without herbicide. Bacterioferritin comigratory protein and glutathione peroxidase-reductase appear to be responsible for detoxification of bentazone-derived peroxide in Mu2.
Department of Biological Sciences, Rani Durgavati University, Jabalpur, Madhya Pradesh, India.
Palash Kumar
PK
Das
Suvendra Nath
SN
Bagchi
D061245:Axenic Culture; D001426:Bacterial Proteins / Q000378:metabolism*; D001581:Benzothiadiazines / Q000494:pharmacology*; D015152:Blotting, Northern; D003573:Cytochrome b Group / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D004789:Enzyme Activation; D005293:Ferritins / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D054464:Peroxiredoxins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D046940:Synechococcus / Q000187:drug effects* / Q000201:enzymology; D013997:Time Factors
D001426:Bacterial Proteins; D001581:Benzothiadiazines; D003573:Cytochrome b Group; D012333:RNA, Messenger; D005293:Ferritins; C018032:bacterioferritin; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; C002003:bentazone
Nature. 1970 Aug 15;227(5259):680-5
5432063
Photosynth Res. 2007 Apr;92(1):87-101
17554635
Environ Toxicol. 2010 Oct;25(5):517-26
20549627
J Bacteriol. 1961 Sep;82:442-8
13714587
J Exp Bot. 2005 Jan;56(411):337-46
15310815
Trends Biochem Sci. 2001 Nov;26(11):648-53
11701322
J Exp Bot. 2005 Dec;56(422):3193-206
16284092
Photosynth Res. 2003;75(2):171-82
16245087
Arch Microbiol. 2009 Feb;191(2):141-51
18974976
Planta. 1978 Jan;139(1):9-17
24414099
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Curr Biol. 2003 Feb 4;13(3):230-5
12573219
Protoplasma. 2009 Mar;235(1-4):3-15
19219525
Aquat Toxicol. 2009 Feb 19;91(3):262-9
19131120
Chemosphere. 2009 Dec;77(11):1501-7
19879624
J Microbiol. 2009 Feb;47(1):60-7
19229492
Microbiology (Reading). 2007 Aug;153(Pt 8):2505-2517
17660415
Toxicol In Vitro. 2008 Apr;22(3):716-22
18180139
Microbiology (Reading). 1999 Jun;145 ( Pt 6):1473-1484
10411274
J Microbiol. 2005 Apr;43(2):191-5
15880096
Anal Biochem. 1984 Dec;143(2):226-30
6532239
Anal Biochem. 1980 Jul 15;106(1):207-12
7416462
Environ Toxicol. 2010 Apr;25(2):147-56
19319991
Mikrobiologiia. 2006 Nov-Dec;75(6):765-74
17205801
BMC Mol Biol. 2009 Aug 07;10:79
19660145
FEBS Lett. 2006 Nov 13;580(26):6055-61
17054949
Br J Haematol. 1973 Nov;25(5):563-75
4518624
J Biol Chem. 1974 Nov 25;249(22):7130-9
4436300
Toxicol Mech Methods. 2009 Feb;19(2):109-15
19778254
Plant J. 2006 Mar;45(6):968-81
16507087
Methods Enzymol. 1984;105:121-6
6727660