A rational approach to tuning the pKa values of rhodamines for living cell fluorescence imaging.
A novel strategy to systematically tune the pK(a) values of rhodamines is described. This strategy was applied to rationally develop compound 1e with a pK(a) of 6.5, the highest among rhodamine amide derivatives, and it could be employed to detect acidic pH variations in living cells with a turn-on signal.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China.
Lin
L
Yuan
Weiying
W
Lin
Yanming
Y
Feng
D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry*
D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G