Immunohistochemical evidence of Muc1 expression during rat embryonic development.
During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this experiment, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 d) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 d, liver and esophagus and at 18 d, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
Centre for Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, Argentina.
E
E
Lacunza
V
V
Ferretti
C
C
Barbeito
A
A
Segal-Eiras
M V
MV
Croce
D000818:Animals; D047108:Embryonic Development / Q000502:physiology*; D004848:Epithelium / Q000196:embryology; D004947:Esophagus / Q000196:embryology; D005260:Female; D005333:Fetus / Q000196:embryology*; D007124:Immunoenzyme Techniques; D007421:Intestine, Small; D007668:Kidney / Q000196:embryology; D008099:Liver / Q000196:embryology; D008168:Lung / Q000196:embryology; D018396:Mucin-1 / Q000378:metabolism*; D010179:Pancreas / Q000196:embryology; D011247:Pregnancy; D051381:Rats / Q000196:embryology*; D012469:Salivary Glands / Q000196:embryology; D013270:Stomach / Q000196:embryology; D014132:Trachea / Q000196:embryology
D018396:Mucin-1
Biochim Biophys Acta. 1999 Oct 8;1455(2-3):301-13
10571020
Mamm Genome. 1995 Dec;6(12):885-8
8747930
Biochim Biophys Acta. 1995 Dec 20;1241(3):407-23
8547303
Annu Rev Cell Dev Biol. 2002;18:463-93
12142280
J Histochem Cytochem. 2003 Jun;51(6):781-8
12754289
Differentiation. 1993 Jul;53(3):163-72
8405767
Int J Cancer. 1984 Dec 15;34(6):763-8
6210253
J Mammary Gland Biol Neoplasia. 2001 Jul;6(3):339-53
11547902
Am J Physiol Regul Integr Comp Physiol. 2004 Dec;287(6):R1505-16
15345472
Histol Histopathol. 2006 Aug;21(8):849-55
16691537
Biochem Biophys Res Commun. 2001 May 11;283(3):715-20
11341784
Development. 1992 Jun;115(2):427-37
1425334
J Biol Chem. 2008 Oct 3;283(40):26985-95
18625714
Pediatr Surg Int. 2008 May;24(5):561-5
18351364
Gene. 2006 May 24;373:28-34
16500040
J Immunol. 1994 Sep 1;153(5):2102-9
8051415
Hybridoma. 1984 Fall;3(3):223-32
6094338
Am J Pathol. 1997 Nov;151(5):1273-80
9358753
Anticancer Res. 1997 Nov-Dec;17(6D):4287-92
9494522
Biochem Biophys Res Commun. 2003 May 23;305(1):46-53
12732194
J Biol Chem. 1990 Sep 5;265(25):15294-9
2394722
Int J Cancer. 1984 Mar 15;33(3):299-304
6365800
Cancer Res. 2001 Sep 15;61(18):6846-50
11559560
Respir Med. 2003 Aug;97(8):964-9
12924525
Nat Rev Cancer. 2004 Jan;4(1):45-60
14681689
J Mol Histol. 2008 Oct;39(5):519-25
18784978
J Pathol. 2001 Jul;194(3):373-83
11439371
World J Gastroenterol. 2005 Aug 14;11(30):4661-6
16094706
Anat Rec. 1998 Apr;250(4):459-64
9566536
J Biol Chem. 1991 Nov 15;266(32):21537-47
1718981
Appl Immunohistochem Mol Morphol. 2010 Jan;18(1):41-50
19625949
Dev Growth Differ. 2000 Apr;42(2):145-53
10830438
Proc Natl Acad Sci U S A. 1987 Sep;84(17):6060-4
2888110
Oncogene. 2010 Feb 11;29(6):920-9
19915608
Cell Tissue Res. 2008 Nov;334(2):219-26
18855019
Am J Respir Cell Mol Biol. 1993 May;8(5):546-55
7683198
Res Vet Sci. 2009 Feb;86(1):68-77
18582913