Expansion of nanotechnology for dentistry: effect of colloidal platinum nanoparticles on dentin adhesion mediated by 4-META/MMA-TBB.
OBJECTIVE
To investigate the effect of Colloidal Platinum Nanoparticles (CPN) on the bond strength between dentin and 4-META/MMA-TBB resin using different concentrations of CPN.


METHODS
Twenty-five extracted human third molars were stored in 0.5% chloramine T. The occlusal dentin slices were prepared by grinding occlusal surfaces of each tooth and polishing with 600-grit silicon carbide paper under running water. One control and four experimental groups (2 specimens per group) were used as follows: a) dentin surfaces treated with 10-3 solution, followed by rinsing with water and subsequently an acrylic rod bonded with hand-mixed 4META/MMA-TBB resin (Super-Bond C&B, Sun Medical) (control); b) dentin surfaces treated with 10-3 etching solution, followed by rinsing with water and application of CPN (100% or 10%) as a primer solution for 60 s and rinsed with water for 20 s, then an acrylic rod bonded with Super-Bond C&B(Etch-CPN [100% or 10%]); c) dentin surfaces treated with CPN (100% or 10%) for 60 s, rinsed with water for 20 s, followed by application of 10-3 solution, then an acrylic rod bonded with Super-Bond C&B (CPN-Etch [100% or 10%]). After storage in 37°C water, specimens were sectioned into beams (cross-sectional area: 1 mm2) for microtensile bond strength testing at a crosshead speed of 1mm/min. The data were analyzed using the Games-Howell method (p < 0.05; n = 15).


RESULTS
Etch-CPN (100), CPN-Etch(100) and CPN-Etch (10) showed significantly higher bond strengths compared to the control. When using 10% CPN, the highest bond strength was demonstrated. The bond strength of 4META/MMA-TBB resin was approximately doubled by CPN application.


CONCLUSIONS
The results of this study showed that higher bond strengths are obtained when treating dentin with a lower concentration of CPN. Further evaluation to optimize conditions such as the application time and rinsing time are required.
Department of Restorative Dentistry, Hokkaido University Graduate School of Dentistry, Sapporo, Japan. hoshika@den.hokudai.ac.jp
Shuhei
S
Hoshika
Futami
F
Nagano
Toru
T
Tanaka
Takahiro
T
Wada
Kiyotaka
K
Asakura
Kenichi
K
Koshiro
Denis
D
Selimovic
Yusei
Y
Miyamoto
S K
SK
Sidhu
Hidehiko
H
Sano
D000134:Acid Etching, Dental / Q000379:methods; D000180:Acrylic Resins / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D001840:Dental Bonding; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D008768:Methylmethacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D010984:Platinum / Q000737:chemistry*; D019279:Resin Cements / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013718:Tensile Strength; D014867:Water / Q000737:chemistry
D000180:Acrylic Resins; D001896:Boron Compounds; D017554:Carbon Compounds, Inorganic; D003102:Colloids; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D008768:Methylmethacrylates; D019279:Resin Cements; D017655:Silicon Compounds; D014867:Water; D010984:Platinum; C049495:Super-bond; C022088:silicon carbide