A pilot study on lignocelluloses to ethanol and fish feed using NMMO pretreatment and cultivation with zygomycetes in an air-lift reactor.
A complete process for the production of bioethanol and fungal biomass from spruce and birch was investigated. The process included milling, pretreatment with N-methylmorpholine-N-oxide (NMMO), washing of the pretreated wood, enzymatic hydrolysis, and cultivation of the zygomycetes fungi Mucor indicus. Investigated factors included wood chip size (0.5-16 mm), pretreatment time (1-5h), and scale of the process from bench-scale to 2m high air-lift reactor. Best hydrolysis yields were achieved from wood chips below 2mm after 5h of pretreatment. Ethanol yields (mg/g wood) of 195 and 128 for spruce, and 175 and 136 for birch were achieved from bench-scale and airlift, respectively. Fungal biomass yields (mg/g wood) of 103 and 70 for spruce, and 86 and 66 for birch from bench scale and airlift respectively were simultaneously achieved. NMMO pretreatment and cultivation with M. indicus appear to be a good alternative for ethanol production from birch and spruce.
School of Engineering, University of Borås, 501 90 Borås, Sweden. Patrik.Lennartsson@hb.se
Patrik R
PR
Lennartsson
Claes
C
Niklasson
Mohammad J
MJ
Taherzadeh
D000704:Analysis of Variance; D000821:Animal Feed / Q000032:analysis*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism*; D009025:Morpholines / Q000494:pharmacology*; D009089:Mucor / Q000187:drug effects / Q000254:growth & development*; D010865:Pilot Projects; D013997:Time Factors; D014934:Wood / Q000737:chemistry / Q000187:drug effects
D002241:Carbohydrates; D003497:Cyclic N-Oxides; D009025:Morpholines; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; C067960:4-methylmorpholine N-oxide; D005947:Glucose; D005990:Glycerol