Carica papaya lipase: a naturally immobilized enzyme with interesting biochemical properties.
Triacylglycerol (TAG) lipases have been thoroughly characterized in mammals and microorganisms, whereas very little is known about plant TAG lipases. The lipolytic activity occurring in all the laticies is known to be associated with sedimentable particles, and all attempts to solubilize the lipolytic activity of Carica papaya latex have been unsuccessful so far. However, some of the biochemical properties of the lipase from Carica papaya latex (CPL) were determined from the insoluble fraction of the latex. The activity was optimum at a temperature of 37°C and a pH of 9.0, and the specific activities of CPL were found to be 2,000 ± 185 and 256 ± 8 U/g when tributyrin and olive oil were used as substrates, respectively. CPL was found to be active in the absence of any detergent, whereas many lipases require detergent to prevent the occurrence of interfacial denaturation. CPL was inactive in the presence of micellar concentrations of Triton X-100, sodium dodecyl sulfate (SDS) and tetradecyl trimethylammonium bromide (TTAB), and still showed high levels of activity in the presence of sodium taurodeoxycholate (NaTDC) and the zwitterionic Chaps detergent. The effects of various proteases on the lipolytic activity of CPL were studied, and CPL was found to be resistant to treatment with various enzymes, except in the presence of trypsin. All these properties suggest that CPL may be a good candidate for various biotechnological applications.
CNRS-Aix-Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UPR9025, Marseille, France. slim.abdelkafi@yahoo.fr
Slim
S
Abdelkafi
Nathalie
N
Barouh
Benjamin
B
Fouquet
Imen
I
Fendri
Michel
M
Pina
Frantz
F
Scheirlinckx
Pierre
P
Villeneuve
Frédéric
F
Carrière
D029441:Carica / Q000201:enzymology*; D003902:Detergents / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007840:Latex / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008066:Lipolysis / Q000187:drug effects; D017830:Octoxynol / Q000737:chemistry; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013379:Substrate Specificity; D013657:Taurodeoxycholic Acid / Q000737:chemistry; D014280:Triglycerides / Q000378:metabolism; D014357:Trypsin
D003902:Detergents; D004800:Enzymes, Immobilized; D007840:Latex; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D012967:Sodium Dodecyl Sulfate; D013657:Taurodeoxycholic Acid; D017830:Octoxynol; D008049:Lipase; D014357:Trypsin; C005830:tributyrin
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