A new prodrug-derived ratiometric fluorescent probe for hypoxia: high selectivity of nitroreductase and imaging in tumor cell.
Based on the hypoxia prodrug moiety of p-nitrobenzyl, a selective ratiometric fluorescent sensor (RHP) for the detection of microenvironment hypoxia was designed and synthesized. RHP can be selectively activated by bioreductive enzymes (NTR) and results in an evident blue to green fluorescent emission wavelength change in both solution phases and in cell lines, which might be the first fluorescent ratiometric probe for hypoxia in solid tumors.
State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology (ECUST), Shanghai, China.
Lei
L
Cui
Ye
Y
Zhong
Weiping
W
Zhu
Yufang
Y
Xu
Qingshan
Q
Du
Xin
X
Wang
Xuhong
X
Qian
Yi
Y
Xiao
D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis*; D015394:Molecular Structure; D009369:Neoplasms / Q000628:therapy; D009574:Nitro Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D009601:Nitroreductases / Q000378:metabolism*; D011355:Prodrugs / Q000378:metabolism*
D005456:Fluorescent Dyes; D009574:Nitro Compounds; D011355:Prodrugs; D009601:Nitroreductases