Pseudomonas aeruginosa PAO1 adapted to 2-phenoxyethanol shows cross-resistance to dissimilar biocides and increased susceptibility to antibiotics.
The growth adaptability to increasing concentration of the biocide 2-phenoxyethanol (PE) was determined in Pseudomonas aeruginosa PAO1 (P.a.) as part of efforts to understand and control the biocide tolerance and its effect on cross-resistance to other biocides and resistance to antibiotics. After repeated subculturing in media containing increasing sub-minimum-inhibitory PE concentration, P.a. exhibited an adaptive resistance indicated by two-fold increase in MIC at the 10th passage. The resistance was stable and remained after passaging the strain in further 7 successive passages in PE-free growth media. The strain showed cross-resistance towards dissimilar biocides and displayed increased susceptibility to antibiotics, which was not influenced by the presence of the efflux inhibitor 'carbonyl cyanide m-chlorophenyl hydrazone'. Outer membranes of adapted strain showed altered protein profile when examined by SDS-PAGE.
Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Biomedical Sciences, Petra University, Amman, Jordan. mlrsam2001@yahoo.com
S M A
SM
Abdel Malek
Y R
YR
Badran
D000220:Adaptation, Biological; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004202:Disinfectants / Q000378:metabolism / Q000633:toxicity*; D004361:Drug Tolerance; D004591:Electrophoresis, Polyacrylamide Gel; D005026:Ethylene Glycols / Q000378:metabolism / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D020543:Proteome / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D012692:Serial Passage
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D004202:Disinfectants; D005026:Ethylene Glycols; D020543:Proteome; C005398:phenoxyethanol
J Appl Bacteriol. 1993 Jan;74(1):94-9
8420922
J Antimicrob Chemother. 2002 Apr;49(4):631-9
11909837
Curr Microbiol. 2009 Aug;59(2):95-100
19452215
J Antimicrob Chemother. 1990 Jun;25(6):921-9
2370240
Antimicrob Agents Chemother. 2001 Apr;45(4):1284-6
11257048
Clin Microbiol Rev. 2003 Apr;16(2):189-208
12692093
J Hosp Infect. 2000 Dec;46(4):297-303
11170761
J Appl Microbiol. 2000 Aug;89(2):289-95
10971761
J Antimicrob Chemother. 2003 Nov;52(5):750-63
14519671
Drug Resist Updat. 2000 Aug;3(4):247-255
11498392
J Appl Bacteriol. 1994 Jun;76(6):576-82
8027006
J Appl Microbiol. 2002;92(4):729-36
11966914
J Infect. 2001 Jul;43(1):85-91
11597168
Appl Environ Microbiol. 1985 Jan;49(1):1-7
3883894
J Gen Microbiol. 1986 Dec;132(12):3253-9
3309133