A plant cocktail amenable for PCR-based genetic analysis in Arabidopsis thaliana.
Extraction of plant genomic DNA for subsequent genetic studies is often lengthy and difficult. In addition, it requires the use of toxic reagents that remove secondary plant products, which otherwise interfere with the polymerase chain reaction steps. Here we describe a simple cost-efficient one-step protocol for PCR-based analysis in Arabidopsis thaliana. This protocol is quick, performed at room temperature without the need for DNA extraction. Potential applications in higher plants are here discussed.
Department of Agronomy, Purdue University, Lilly Hall, 915 West State Street, West Lafayette, IN 47907, USA. skotchon@purdue.edu
Simeon O
SO
Kotchoni
Emma W
EW
Gachomo
Jose C
JC
Jimenez-Lopez
D017360:Arabidopsis / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*
D018744:DNA, Plant
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