Enantioselective separation and electrochemical sensing of D- and L-tryptophan at ultratrace level using molecularly imprinted micro-solid phase extraction fiber coupled with complementary molecularly imprinted polymer-fiber sensor.
Highly efficient enantioselective separation and quantitative recoveries of D- and L-tryptophan in aqueous and real samples can be achieved, with a monolithic molecularly imprinted polymeric fiber that serves both for micro-solid phase extraction and ultratrace sensing, without any false-positive (non-specific) contribution and cross-reactivity, in the range of 0.15-30.00 ng mL(-1) with detection limit as low as 0.0261 ng mL(-1) (relative standard deviation=0.64%, signal/noise=3). The proposed method combining molecularly imprinted micro-solid phase extraction fiber and a complementary molecularly imprinted polymer-carbon composite fiber sensor is proven to be useful for clinical diagnosis of stress-related diseases caused by acute tryptophan depletion.
Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India. prof.bbpd@yahoo.com
Bhim Bali
BB
Prasad
Mahavir Prasad
MP
Tiwari
Rashmi
R
Madhuri
Piyush Sindhu
PS
Sharma
D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D006801:Humans; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011108:Polymers; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013237:Stereoisomerism; D014364:Tryptophan / Q000032:analysis / Q000097:blood / Q000737:chemistry*
D011108:Polymers; D014364:Tryptophan