Time-dependent responses of rat troponin I and cardiac injury following isoproterenol administration.
OBJECTIVE
To develop a rat model of myocardial infarction induced by isoproterenol (ISO). We investigated a type of histological myocardial changes and cardiac troponin I (TnI) kinetic.


METHODS
The study has used adult, male, Wistar strain rats. Rats were distributed in ISO and control groups. Rats treated with ISO were divided into groups according to the time of cTnI and myocardial lesion analyses: ISO I (30'), ISO II (60'), ISO III (120') and ISO IV (240'). We determined cTnI (Life Diagnostics Inc. West Chester PA, USA) in the serum by ELISA method. We performed histological analysis on the specimens of left ventricular wall stained by hematoxillin-eosin (HE) method.


RESULTS
The first statistically significant rise of cTnI was noted 30 minutes after the ISO administration. There was no statistically significant difference between cTnI mean values among the ISO groups. Observed myocardial histological changes were time dependent.


CONCLUSIONS
This model can be suitable for cardioprotective and cardiotoxicity supstance investigations followed by cTnI measurement in blood. The similarity between induced myocardial lesion on animal model in our study and human myocardial lesion in ischemia give us sufficient impulse for further preclinical researches of new cardiac markers.
Institute for Physiology and Biochemistry, Medical Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. sabaheta.hasic@mf.unsa.ba
Sabaheta
S
Hasić
Radivoj
R
Jadrić
Emina
E
Kiseljaković
Amina
A
Valjevac
Zakira
Z
Mornjaković
Mira
M
Winterhalter-Jadrić
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002316:Cardiotonic Agents / Q000633:toxicity*; D004195:Disease Models, Animal; D006321:Heart / Q000187:drug effects*; D007545:Isoproterenol / Q000633:toxicity*; D008297:Male; D009203:Myocardial Infarction / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D019210:Troponin I / Q000097:blood*
D015415:Biomarkers; D002316:Cardiotonic Agents; D019210:Troponin I; D007545:Isoproterenol