Structural plasticity of staphylococcal nuclease probed by perturbation with pressure and pH.
The ionization of internal groups in proteins can trigger conformational change. Despite this being the structural basis of most biological energy transduction, these processes are poorly understood. Small angle X-ray scattering (SAXS) and nuclear magnetic resonance (NMR) spectroscopy experiments at ambient and high hydrostatic pressure were used to examine how the presence and ionization of Lys-66, buried in the hydrophobic core of a stabilized variant of staphylococcal nuclease, affect conformation and dynamics. NMR spectroscopy at atmospheric pressure showed previously that the neutral Lys-66 affects slow conformational fluctuations globally, whereas the effects of the charged form are localized to the region immediately surrounding position 66. Ab initio models from SAXS data suggest that when Lys-66 is charged the protein expands, which is consistent with results from NMR spectroscopy. The application of moderate pressure (<2 kbar) at pH values where Lys-66 is normally neutral at ambient pressure left most of the structure unperturbed but produced significant nonlinear changes in chemical shifts in the helix where Lys-66 is located. Above 2 kbar pressure at these pH values the protein with Lys-66 unfolded cooperatively adopting a relatively compact, albeit random structure according to Kratky analysis of the SAXS data. In contrast, at low pH and high pressure the unfolded state of the variant with Lys-66 is more expanded than that of the reference protein. The combined global and local view of the structural reorganization triggered by ionization of the internal Lys-66 reveals more detectable changes than were previously suggested by NMR spectroscopy at ambient pressure.
College of Pharmaceutical Sciences, Ritsumeikan University, Shiga, Japan.
Ryo
R
Kitahara
Kazumi
K
Hata
Akihiro
A
Maeno
Kazuyuki
K
Akasaka
Michael S
MS
Chimenti
Bertrand
B
Garcia-Moreno E
Martin A
MA
Schroer
Christoph
C
Jeworrek
Metin
M
Tolan
Roland
R
Winter
Julien
J
Roche
Christian
C
Roumestand
Karine
K
Montet de Guillen
Catherine A
CA
Royer
D019943:Amino Acid Substitution; D041961:Deuterium Exchange Measurement; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011312:Pressure; D011487:Protein Conformation; D058767:Protein Unfolding; D053838:Scattering, Small Angle; D055672:Static Electricity; D014961:X-Ray Diffraction
D008836:Micrococcal Nuclease; D008239:Lysine
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