Effect of heme oxygenase-1 on renal function in rats with liver cirrhosis.
OBJECTIVE
To investigate the role of heme oxygenase-1 (HO-1) in pathogenesis of experimental hepatorenal syndrome (HRS).


METHODS
Rats were divided into liver cirrhotic group, zinc protoporphyrin IX (ZnPP) treatment group, cobalt protoporphyrin (CoPP) treatment group and sham group. Biliary cirrhosis was established by bile duct ligation in the first three groups. Rats in the ZnPP and CoPP treatment groups received intraperitoneal injection of ZnPP and CoPP, respectively, 24 h before sample collection. Expression of HO-1 mRNA in kidney was detected by reverse-transcription polymerase chain reaction, while protein expression was determined by immunohistochemical analysis. Hematoxylin and eosin staining was performed to observe liver cirrhosis and renal structure. Renal artery blood flow, mean arterial pressure and portal vein pressure, 24 h total urinary volume, serum and urine sodium concentrations, and creatinine clearance rate (Ccr) were also measured.


RESULTS
The HO-1 mRNA and protein expression levels in kidney, 24 h total urinary volume, renal artery blood flow, serum and urine sodium concentration and Ccr were lower in cirrhotic group than in sham group (P < 0.05). However, they were significantly lower in ZnPP treatment group than in cirrhotic group and significantly higher in CoPP treatment group than in cirrhotic group (P < 0.05).


CONCLUSIONS
Low HO-1 expression level in kidney is an important factor for experimental HRS.
Department of Gastroenterology, the First Affiliated Hospital of Dalian Medical University, Dalian 116001, Liaoning Province, China.
Shi-Bin
SB
Guo
Zhi-Jun
ZJ
Duan
Qing
Q
Li
Xiao-Yu
XY
Sun
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006439:Hemodynamics; D006530:Hepatorenal Syndrome / Q000473:pathology* / Q000503:physiopathology*; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007677:Kidney Function Tests; D008103:Liver Cirrhosis / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004791:Enzyme Inhibitors; D011524:Protoporphyrins; D012333:RNA, Messenger; C017803:zinc protoporphyrin; D051547:Heme Oxygenase-1
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