Assessment of in vitro dermal absorption of the kaurenic acid from Coespeletia moriziana extracts.
Espeletiinae are plants which grow above 3000 m of altitude in the Northern Andes and kaurenic acid was extracted from the leaves of Coespeletia moritziana. This compound has shown a wide range of biological activities, including cytotoxicity which is efficient in cancer therapy. The percutaneous penetration of this compound was measured in vitro using Franz cells. At appropriate intervals for up to 24h, diffusion samples were analyzed using HPLC. At the end of the test period, the amount of kaurenic acid was determined in each compartment and approximately 10% of kaurenic acid had been absorbed and was found in the skin layers.
Faculté de Pharmacie, Laboratoire de Chimie analytique, Université de Montpellier I, France.
J P
JP
Mestres
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A
Usubillaga
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L
Diaz
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M
Larroque
L
L
Vian
G
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Marti-Mestres
D000279:Administration, Cutaneous; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D004224:Diterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics; D012869:Skin Absorption; D013552:Swine
D004224:Diterpenes; D010936:Plant Extracts; C051436:kaurenoic acid