[Effects of intraprostatic injection of botulinum toxin A (BTX-A) on benign prostate hyperplasia].
OBJECTIVE
To investigate the effects of intraprostatic injection of botulinum toxin A (BTX-A) on benign prostate hyperplasia (BPH) in rats.


METHODS
Models of BPH were established in adult male Sprague-Dawley rats by injection of testosterone propionate, and then divided into three BTX-A groups, injected with BTX-A into the ventral prostate at the doses of 5 U, 10 U and 20 U, a negative control group, injected with saline only, and a sham operation group, with 12 in each. The prostates of the animals were harvested at 2 or 4 weeks after the injection, their volumes and weights measured, histological changes examined by HE staining, and glandular and interstitial areas semi-quantified by the image analysis system.


RESULTS
Two rats died in the 20 U group within 3 days after BTX-A injection. Compared with the saline group, the 5 U, 10 U and 20 U BTX-A groups showed significant decreases in prostatic volume (P < 0.01, 0.01 and 0.05), weight, and glandular and interstitial areas as well as atrophic epithelia in the glandular tube at 2 weeks. These changes were lessened at 4 weeks, especially in the 5 U group.


CONCLUSIONS
Intraprostatic injection of BTX-A induces obvious atrophy and histological changes of the prostate, but meanwhile may potentially result in death at a large dose.
Department of Urology, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei 430070, China. urologydepartment@yahoo.cn
Yao-Peng
YP
Xu
Xiao
X
Yu
Zhang-Qun
ZQ
Ye
Tie-Jun
TJ
Pan
Han-Dong
HD
Wen
Tao
T
Wang
D000818:Animals; D019274:Botulinum Toxins, Type A / Q000008:administration & dosage / Q000627:therapeutic use* / Q000633:toxicity; D008297:Male; D011467:Prostate / Q000187:drug effects* / Q000473:pathology; D011470:Prostatic Hyperplasia / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D019274:Botulinum Toxins, Type A