[Increase in glucoamylase productivity of Aspergillus awamori strain by combination of radiating mutagenesis and plasmid transformation methods].
Increase in the level of amylolytic genes activator protein encoded by amyR gene was shown to result in enhancement of glucoamylase productivity of A. awamori strain by 30%. However, the same effect equal to 30% increase can be achieved by introduction of extra copies of gla gene encoding glucoamylase. These two effects were not additive, which gave the possibility to suggest an additional limitation in the egulation mechanism of glucoamylase gene expression in Aspergillus family strains while introducing an additional copies of amyR and gla genes.
Iu P
IuP
Vinetskiĭ
A M
AM
Rozhkova
A S
AS
Sereda
N V
NV
Tsurikova
A K
AK
Nurtaeva
M V
MV
Semenova
I N
IN
Zorov
A P
AP
Sinitsyn
D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D001709:Biotechnology; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005720:Gamma Rays; D005818:Genetic Engineering / Q000379:methods*; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D015534:Trans-Activators / Q000235:genetics; D014170:Transformation, Genetic
D005656:Fungal Proteins; D015534:Trans-Activators; C414005:amyR protein, Aspergillus; D005087:Glucan 1,4-alpha-Glucosidase