Cholesterol is not an essential source of nutrition for Mycobacterium tuberculosis during infection.
Rv1106c (hsd; 3β-hydroxysteroid dehydrogenase) is required by Mycobacterium tuberculosis for growth on cholesterol as a sole carbon source, whereas Rv3409c is not. Mutation of Rv1106c does not reduce Mycobacterium tuberculosis growth in infected macrophages or guinea pigs. We conclude that cholesterol is not required as a nutritional source during infection.
Department of Chemistry, Stony Brook University, Stony Brook, NY 11794-3400, USA.
Xinxin
X
Yang
Jin
J
Gao
Issar
I
Smith
Eugenie
E
Dubnau
Nicole S
NS
Sampson
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002784:Cholesterol / Q000378:metabolism*; D004195:Disease Models, Animal; D017353:Gene Deletion; D006168:Guinea Pigs; D008264:Macrophages / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000378:metabolism* / Q000472:pathogenicity*; D014376:Tuberculosis / Q000382:microbiology*; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
D001426:Bacterial Proteins; D037521:Virulence Factors; D002784:Cholesterol
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