Sequential measurements of glomerular filtration rate in conscious rats by a bolus injection of iodixanol and a single blood sample.
To estimate the glomerular filtration rate (GFR) sequentially in conscious rats, we validated a single-blood-sample method using a bolus injection of the nonionic contrast medium iodixanol as a tracer. First, to clarify basal age-related GFR profiles, iodixanol was intravenously administered once weekly at 1500 mg kg(-1) I to clinically healthy male F344 rats from 6 to 15 weeks of age. The blood sample was collected 120 min later, and serum iodixanol concentration was measured by HPLC. GFR values decreased gradually by 9 weeks, presumably due to rapidly increased body weights, and then remained constant from 10 weeks onward. When converting the GFR from body weight to body surface area, the reference range (40-60 ml min(-1) m(-2) ) in the latter was much more stable than that (6-11 ml min(-1) kg(-1) ) in the former. For nephropathy rats induced by cisplatin (3.75-7.5 mg kg(-1) , i.v., single dose), bromoethylamine hydrobromide (BEA, 250-500 mg kg(-1) , i.v., single dose) or puromycin aminonucleoside (PAN, 15 mg kg(-1) day(-1) , s.c., 10 days), GFR decreased or tended to decrease before increasing in serum urea nitrogen (UN) and creatinine concentrations. Accordingly, serum UN and creatinine concentrations became elevated when the GFR decreased to 50-60% of the basal value. This method without urine collection contributes to the reduction of animal numbers because of repeated application to the same animals.
Department of Veterinary Basic Medicine, Iwate University, Morioka, Iwate, 020-8550, Japan; United Graduate School of Veterinary Science, Gifu University, Gifu, 501-1193, Japan.
Rieko
R
Katayama
Kanji
K
Watanabe
Norio
N
Yamagishi
Sumito
S
Abe
Hiroshi
H
Satoh
Kazuhisa
K
Furuhama
D000375:Aging / Q000097:blood*; D023401:Animal Use Alternatives; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003287:Contrast Media; D005919:Glomerular Filtration Rate; D007275:Injections, Intravenous; D007674:Kidney Diseases / Q000097:blood* / Q000139:chemically induced; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D014283:Triiodobenzoic Acids / Q000097:blood*
D003287:Contrast Media; D014283:Triiodobenzoic Acids; C044834:iodixanol