Development of a cellular assay system to study the genome replication of high- and low-risk mucosal and cutaneous human papillomaviruses.
We found that recircularized high-risk (type 16 and 18) and low-risk mucosal (type 6b and 11) and cutaneous (type 5 and 8) human papillomavirus (HPV) genomes replicate readily when delivered into U2OS cells by electroporation. The replication efficiency is dependent on the amount of input HPV DNA and can be followed for more than 3 weeks in proliferating cell culture without selection. Cotransfection of recircularized HPV genomes with a linear G418 resistance marker plasmid has allowed subcloning of cell lines, which, in a majority of cases, carry multicopy episomal HPV DNA. Analysis of the HPV DNA status in these established cell lines showed that HPV genomes exist in these cells as stable extrachromosomal oligomers. When the cell lines were cultivated as confluent cultures, a 3- to 10-fold amplification of the HPV genomes per cell was induced. Two-dimensional (2D) agarose gel electrophoresis confirmed amplification of mono- and oligomeric HPV genomes in these confluent cell cultures. Amplification occurred as a result of the initiation of semiconservative two-dimensional replication from one active origin in the HPV oligomer. Our data suggest that the system described here might be a valuable, cost-effective, and efficient tool for use in HPV DNA replication studies, as well as for the design of cell-based assays to identify potential inhibitors of all stages of HPV genome replication.
Department of Biomedical Technology, Institute of Technology, University of Tartu, Nooruse 1, 50411, Tartu, Estonia.
Jelizaveta
J
Geimanen
Helen
H
Isok-Paas
Regina
R
Pipitch
Kristiina
K
Salk
Triin
T
Laos
Marit
M
Orav
Tormi
T
Reinson
Mart
M
Ustav
Mart
M
Ustav
Ene
E
Ustav
D002460:Cell Line; D004270:DNA, Circular / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D018274:Electroporation; D016679:Genome, Viral; D006801:Humans; D009092:Mucous Membrane / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification / Q000502:physiology*; D030361:Papillomavirus Infections / Q000821:virology*; D012867:Skin / Q000821:virology*; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
D004270:DNA, Circular
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