In vitro genotoxicity of rocuronium bromide in human peripheral lymphocytes.
Rocuronium bromide (RB), an aminosteroid type neuromuscular blocking agent, acts by reducing or inhibiting the depolarising effect of acetylcholine on the terminal disc of the muscle cell. To our knowledge, there is no adequate information on the genotoxic effects of RB, up to now. In the present study, possible genotoxic effects of RB have been determined by means of sister chromatid exchange (SCE), chromosome aberration (CA) and micronucleus (MN) analyses in human peripheral blood lymphocytes. The human peripheral blood lymphocytes were exposed to three different concentrations of RB (60, 80 and 100 μg/mL) for 24- and 48-h. In this study, RB increased the frequency of CAs, however, did not increase the frequency of SCEs. RB did not decrease the proliferation index (PI) and mitotic index (MI). Accordingly, RB increased the frequency of micronucleus (MN) but did not decrease the nuclear division index (NDI). Findings from this study suggest that rocuronium bromide is clastogenic but not cytotoxic to cultured human peripheral blood lymphocytes.
Department of Biology, Institute of Basic and Applied Sciences, Çukurova University, Adana, Turkey.
Umit
U
Zan
Mehmet
M
Topaktas
Erman Salih
ES
Istifli
Environ Mol Mutagen. 2009 Jul;50(6):435-50
19334052
Eur J Anaesthesiol. 2004 Sep;21(9):715-24
15595584
Mutat Res. 2003 Oct 7;540(2):153-63
14550499
Arch Med Res. 1998 Winter;29(4):285-9
9887544
Mutat Res. 1978 Nov;52(2):199-206
740006
Mutagenesis. 2003 Jul;18(4):345-53
12840108
Acta Anaesthesiol Scand. 2001 Nov;45(10):1196-203
11736669
Br J Anaesth. 1990 Jan;64(1):125-6
1967945
Cancer Res. 2000 Jan 15;60(2):390-4
10667592
Mutat Res. 2000 Aug;463(2):111-72
10913908
Mutat Res. 1988 Mar;198(1):255-9
3352630
Mutat Res. 2001 May;488(2):151-69
11344042
Environ Mol Mutagen. 2000;35(3):202-5
10737955
Mutat Res. 1980 Sep;79(1):19-32
6448958
Mutat Res. 1985 Jan-Feb;155(1-2):71-3
3969084
Environ Mol Mutagen. 2004;43(3):143-58
15065202
Mutat Res. 2000 Nov 20;455(1-2):81-95
11113469
Mutat Res. 2005 Mar 7;581(1-2):181-6
15725617
Acta Anaesthesiol Scand. 2000 Aug;44(7):775-81
10939689
Environ Health Perspect. 2002 Nov;110(11):1077-80
12417477
Anaesthesia. 1988 Mar;43(3):207-9
2896468
Nature. 1979 Feb 15;277(5697):531-4
763341
Hum Genet. 1985;70(2):126-9
2408987
Anesthesiology. 1997 Sep;87(3):690-2
9316977
Anaesth Intensive Care. 1996 Aug;24(4):486-8
8862648
Mol Carcinog. 1998 Sep;23(1):1-12
9766432
Mutat Res. 2002 Jul 25;504(1-2):3-16
12106642
Anesth Analg. 1993 Feb;76(2):423-5
8424526
Cytogenet Cell Genet. 1982;33(1-2):48-56
6811206
Mutat Res. 2009 Mar-Jun;681(2-3):209-229
18845271
Environ Mol Mutagen. 2000;35(3):191-201
10737954
Anesth Analg. 1999 Feb;88(2):462-4
9972775
Anesth Analg. 1984 Sep;63(9):865-6
6465585
Anaesthesia. 1986 Sep;41(9):940-2
2877591
Tsitol Genet. 1984 Jan-Feb;18(1):13-7
6701962