Minimal antimicrobial peptidic sequence from hemoglobin alpha-chain: KYR.
Hemoglobin is an animal protein described as a source of biologically active peptides. Peptic digestion of bovine hemoglobin alpha-chain allowed obtaining peptide fractions with antimicrobial activity. These peptides were purified by reverse-phase High-Performance Liquid Chromatography (HPLC) and characterized by mass spectrometry. The minimal inhibitory concentration and mode of action of these peptides were studied against five bacterial strains including Escherichia coli and Salmonella enteritidis as Gram-negative bacteria and Listeria innocua, Micrococcus luteus and Staphylococcus aureus as Gram-positive bacteria. The action aforementioned peptides were studied on artificial membranes as well. The most active peptides resulted to be the short ones. Consequently, the minimal peptidic sequence necessary for the antibacterial activity was clearly determined: KYR.
Laboratoire ProBioGEM, Bâtiment Polytech-Lille, Boulevard Paul Langevin, 59655 Villeneuve d'Ascq, France.
Lucie
L
Catiau
Johnatan
J
Traisnel
Véronique
V
Delval-Dubois
Nour-Eddine
NE
Chihib
Didier
D
Guillochon
Naïma
N
Nedjar-Arroume
D000890:Anti-Infective Agents / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000737:chemistry*; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000890:Anti-Infective Agents; D006454:Hemoglobins; D008567:Membranes, Artificial; D010455:Peptides