Homocysteine-dependent endothelial dysfunction induced by renal ischemia/reperfusion injury.
BACKGROUND
Elevation of serum homocysteine is considered to contribute to endothelial dysfunction, which is considered to be the initial event in vascular disease following renal transplantation. We sought to investigate whether an association existed between serum homocysteine levels and endothelial dysfunction after renal ischemia/reperfusion (I/R) injury.


METHODS
Acetylcholine (Ach)-induced endothelium-dependent and sodium nitroprusside (SNP)-induced endothelial-independent relaxation responses were determined in thoracic aortas from different I/R groups. A correlation analysis was performed between Ach responses and homocysteine levels.


RESULTS
Long-term I/R injury decreased the responses to acetylcholine and the pD2 values of the concentration response curves compared with controls. While vascular responses to SNP were unchanged among all groups. Homocysteine levels correlated with the pD2 values of acetylcholine among control and I/R groups, indicating that the increase in homocysteine was associated with decreased sensitivity to acetylcholine. In short-term I/R rats, no association was observed between these parameters.


CONCLUSIONS
These data suggest a possible link between serum homocysteine and decreased vascular reactivity to endothelium-dependent relaxation in I/R aorta.
Department of Pharmaceutical Sciences, Jodhpur National University, Narnadi, Jodhpur, Rajasthan, India. yagnikbhalodia@gmail.com
Yagnik S
YS
Bhalodia
Navin R
NR
Sheth
Jitendra D
JD
Vaghasiya
Nurudin P
NP
Jivani
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D006710:Homocysteine / Q000097:blood*; D007668:Kidney / Q000098:blood supply*; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood* / Q000503:physiopathology; D015854:Up-Regulation; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
D014665:Vasodilator Agents; D006710:Homocysteine; D009599:Nitroprusside; D000109:Acetylcholine