Feasibility of using multiphoton excited tissue autofluorescence for in vivo human histopathology.
Rapid and direct imaging of microscopic tissue morphology and pathology can be achieved by multiphoton imaging of intrinsic tissue fluorophores and second harmonic signals. Engineering parameters for developing this technology for clinical applications include excitation levels and collection efficiencies required to obtain diagnostic quality images from different tissue types and whether these levels are mutagenic. Here we provide data on typical average powers required for high signal-to-noise in vivo tissue imaging and assess the risk potential of these irradiance levels using a mammalian cell gene mutation assay. Exposure times of ~16 milliseconds per cell to 760 nm, ~200 fs raster-scanned laser irradiation delivered through a 0.75 NA objective produced negligible mutagenicity at powers up to about 50 mW.
Johanna M
JM
Dela Cruz
Jesse D
JD
McMullen
Rebecca M
RM
Williams
Warren R
WR
Zipfel
Biol Met. 1991;4(4):197-200
1777354
Toxicol In Vitro. 2010 Mar;24(2):558-66
19883747
J Clin Invest. 1963 Jun;42(6):767-76
16695904
Cancer Cells. 1990 Mar;2(3):79-83
2202381
Biochim Biophys Acta. 1991 Jul 30;1084(3):261-8
1888774
Microsc Res Tech. 2007 May;70(5):398-402
17393493
Curr Opin Neurobiol. 2004 Oct;14(5):617-28
15464896
Gastrointest Endosc Clin N Am. 2004 Jul;14(3):595-620, xi
15261205
Mutat Res. 1981 Mar;86(2):193-214
7022191
Nat Methods. 2005 Dec;2(12):941-50
16299479
Mutat Res. 2005 Apr 1;571(1-2):19-31
15748635
J Invest Dermatol. 2006 Mar;126(3):667-75
16374481
Nucleic Acids Res. 2009 Apr;37(6):1951-61
19208641
Nat Med. 2003 Jun;9(6):796-800
12754503
J Microsc. 1997 Jan;185(Pt 1):9-20
9057318
Mutat Res. 1993 Aug;294(2):167-77
7687009
Clin Gastroenterol Hepatol. 2008 Jan;6(1):95-101
18065276
Clin Exp Metastasis. 2009;26(4):357-70
18766302
Photochem Photobiol. 1989 Apr;49(4):407-12
2498913
Opt Express. 2010 Jan 18;18(2):1255-60
20173949
J Cataract Refract Surg. 2010 Mar;36(3):442-8
20202543
Gastrointest Endosc. 2007 Jul;66(1):144-9
17591488
Mutat Res. 1987 Oct;189(2):135-41
3657829
Proc SPIE Int Soc Opt Eng. 2009;7161:
19360140
Gastrointest Endosc. 2005 Nov;62(5):686-95
16246680
Cancer Res. 2003 Apr 1;63(7):1464-9
12670891
J Microsc. 2007 Jun;226(Pt 3):195-206
17535259
Transl Oncol. 2010 Jun 01;3(3):181-94
20563260
Radiat Res. 1997 Apr;147(4):490-4
9092930
Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7075-80
12756303
Opt Express. 2006 Feb 6;14(3):1027-32
19503423
Exp Physiol. 2002 Nov;87(6):737-45
12447453
EMBO Rep. 2003 Dec;4(12):1144-9
14618160
Opt Lett. 2002 Sep 1;27(17):1513-5
18026490
Photochem Photobiol. 1999 Aug;70(2):184-90
10461457