An indole-linked C8-deoxyguanosine nucleoside acts as a fluorescent reporter of Watson-Crick versus Hoogsteen base pairing.
Pyrrole- and indole-linked C(8)-deoxyguanosine nucleosides act as fluorescent reporters of H-bonding specificity. Their fluorescence is quenched upon Watson-Crick H-bonding to dC, while Hoogsteen H-bonding to G enhances emission intensity. The indole-linked probe is ∼ 10-fold brighter and shows promise as a fluorescent reporter of Hoogsteen base pairing.
Department of Chemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Katherine M
KM
Schlitt
Andrea L
AL
Millen
Stacey D
SD
Wetmore
Richard A
RA
Manderville
D020029:Base Pairing; D004247:DNA / Q000737:chemistry; D003849:Deoxyguanosine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006860:Hydrogen Bonding; D007211:Indoles / Q000737:chemistry*; D008958:Models, Molecular
D005456:Fluorescent Dyes; D007211:Indoles; C030374:indole; D004247:DNA; D003849:Deoxyguanosine