Changes in tissue metalloproteinase inhibitor-1 and matrix metalloproteinases during tumor development and metastasizing in mice.
Tissue inhibitor of matrix metalloproteinases type 1, inhibiting the majority of matrix metalloproteinases, can both suppress and stimulate tumor growth. The concentrations and activities of tissue matrix metalloproteinase inhibitor-1 were measured in C57Bl/6 mice during progression and metastasizing of Lewis lung adenocarcinoma. Activities of matrix metalloproteinases in tumor tissue of mice were lower than in liver and lung tissues of intact animals. Serum concentration of tissue inhibitor increased significantly during the development of Lewis lung adenocarcinoma. Macrophage depression (injection of gadolinium chloride associated with a decrease in metastasis number) decreased serum concentration of tissue inhibitor, but it did not attain the control level observed in intact mice. These findings attest to a pleiotropic antitumor effect of tissue matrix metalloproteinase inhibitor-1 reflecting disorders in matrix metalloproteinase regulation during the progress of Lewis lung adenocarcinoma in mice.
Institute of Physiology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia. T.A.Korolenko@physiol.ru
T A
TA
Korolenko
T G
TG
Filatova
V M
VM
Belichenko
T V
TV
Alexeenko
S Ya
SY
Zhanaeva
D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000378:metabolism / Q000503:physiopathology*; D005682:Gadolinium; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis / Q000503:physiopathology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood / Q000378:metabolism*
D061965:Matrix Metalloproteinase Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D005682:Gadolinium; C038958:gadolinium chloride