[Study on inhibitory effects of allicin in mucoepidermoid carcinoma cells].
OBJECTIVE
To observe the anticancer mechanism of allicin by observing the inhibitory effect of allicin on human salivary gland carcinoma cell line MEC-1.


METHODS
Cell proliferation were measured by MTT assay at different doses and different hours. In the meantime, cell cycle was detected via flow cytometry after different dose incubation with different hours.


RESULTS
MTT results showed that the inhibitory rates of MEC-1 proliferations were increased in a concentration-and time-dependent manner. Flow cytometry analysis showed percent-age of MEC-1 cells decreased in G0/G1 phase and increased in G2/M. But there was no evident change in S phase. The cells were mainly blocked in M phase, and the inhibitory effect of the allicin on MEC-1 cells increased with the increasing of concentration and time.


CONCLUSIONS
Allicin can inhibit the growth of MEC-1 cells in vitro dramatically.
Department of Stomatology, Fifth Affiliated Hospital, Zunyi Medical College, Zhuhai 519100, China. wfy660505@sina.com
Fayin
F
Wu
Qiong
Q
Chen
Hefeng
H
Zhou
Jin
J
Huang
D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D006801:Humans; D013441:Sulfinic Acids / Q000494:pharmacology*
D004220:Disulfides; D013441:Sulfinic Acids; C006452:allicin