Cross-species infections of cultured cells by hepatitis E virus and discovery of an infectious virus-host recombinant.
The RNA virus, hepatitis E virus (HEV) is the most or second-most important cause of acute clinical hepatitis in adults throughout much of Asia, the Middle East, and Africa. In these regions it is an important cause of acute liver failure, especially in pregnant women who have a mortality rate of 20-30%. Until recently, hepatitis E was rarely identified in industrialized countries, but Hepatitis E now is reported increasingly throughout Western Europe, some Eastern European countries, and Japan. Most of these cases are caused by genotype 3, which is endemic in swine, and these cases are thought to be zoonotically acquired. However, transmission routes are not well understood. HEV that infect humans are divided into nonzoonotic (types 1, 2) and zoonotic (types 3, 4) genotypes. HEV cell culture is inefficient and limited, and thus far HEV has been cultured only in human cell lines. The HEV strain Kernow-C1 (genotype 3) isolated from a chronically infected patient was used to identify human, pig, and deer cell lines permissive for infection. Cross-species infections by genotypes 1 and 3 were studied with this set of cultures. Adaptation of the Kernow-C1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene.
Section of Molecular Hepatitis, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Priyanka
P
Shukla
Hanh T
HT
Nguyen
Udana
U
Torian
Ronald E
RE
Engle
Kristina
K
Faulk
Harry R
HR
Dalton
Richard P
RP
Bendall
Frances E
FE
Keane
Robert H
RH
Purcell
Suzanne U
SU
Emerson
D000818:Animals; D001483:Base Sequence; D018938:Caco-2 Cells; D003670:Deer / Q000821:virology; D005260:Female; D005838:Genotype; D016751:Hepatitis E / Q000235:genetics* / Q000401:mortality; D016752:Hepatitis E virus / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000235:genetics / Q000401:mortality; D011995:Recombination, Genetic; D012269:Ribosomal Proteins / Q000235:genetics*; D013045:Species Specificity; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000235:genetics / Q000401:mortality
D012269:Ribosomal Proteins
Int J Gynaecol Obstet. 2004 Jun;85(3):240-4
15145258
J Infect Dis. 2010 Sep 15;202(6):819-21
20695795
J Virol. 2004 May;78(9):4838-46
15078965
J Clin Microbiol. 2010 Apr;48(4):1112-25
20107086
Minerva Gastroenterol Dietol. 2010 Jun;56(2):121-8
20485250
Aliment Pharmacol Ther. 2007 Nov 15;26(10):1429-35
17850420
J Infect Dis. 2010 Sep 15;202(6):835-44
20695798
J Clin Microbiol. 2009 Jun;47(6):1906-10
19369433
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2662-6
7708701
J Viral Hepat. 2010 Mar;17(3):153-61
20040046
J Gen Virol. 2009 Aug;90(Pt 8):1880-1891
19339479
N Engl J Med. 2009 Sep 3;361(10):1025-7
19726781
Virol J. 2010 Jun 14;7:129
20546601
Vet Res. 2010 Nov-Dec;41(6):46
20359452
J Virol. 2006 Jun;80(12):5919-26
16731930
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15270-5
11742081
Lancet. 2003 Aug 2;362(9381):371-3
12907011
J Virol. 2010 Sep;84(18):9059-69
20610720
Clin Infect Dis. 2006 Jun 15;42(12):1679-84
16705571
J Gastroenterol Hepatol. 2006 Jul;21(7):1223-4
16824086
Cancer Res. 1982 Sep;42(9):3858-63
6286115
J Biosci. 2008 Nov;33(4):451-64
19208971
J Gen Virol. 2009 Feb;90(Pt 2):457-462
19141456
J Virol. 2009 Jan;83(1):384-95
18945785
J Hepatol. 2008 Mar;48(3):494-503
18192058
JNMA J Nepal Med Assoc. 2009 Jul-Sep;48(175):226-9
20795462