Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification.
Saccharomyces cerevisiae was transformed for higher ethanol tolerance by error-prone whole genome amplification. The resulting PCR products were transformed back to the parental strain for homologous recombination to create a library of mutants with the perturbed genomic networks. A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l(-1) compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l(-1).
Industrial Biotechnology Program, Institute of Chemical and Engineering Sciences, Agency for Science, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island 627833, Singapore.
Annette Lin
AL
Luhe
Lily
L
Tan
Jinchuan
J
Wu
Hua
H
Zhao
D000935:Antifungal Agents / Q000633:toxicity*; D025141:Drug Resistance, Fungal; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D016681:Genome, Fungal; D005947:Glucose / Q000378:metabolism; D050296:Microbial Viability; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic
D000935:Antifungal Agents; D000431:Ethanol; D005947:Glucose