A prototype of giant magnetoimpedance-based biosensing system for targeted detection of gastric cancer cells.
A targeted detection of gastric cancer cells is achieved by combining the giant magnetoimpedance (GMI)-based biosensing system and RGD-4C peptide coupled, chitosan covered superparamagnetic iron oxide particles (RGD-Fe(3)O(4)@chitosan). The micro-patterned GMI sensor for targeted detection is made of Co-based ribbon and fabricated by micro electromechanical system (MEMS) technology. Functionalized nanoparticles were designed by coating Fe(3)O(4) with chitosan and conjugating with RGD-4C peptides. The targeted cells were trickled down into the detection area of the system. The detection of each sample is carried out in ten-fold manner and average value is taken as the final result. This system can identify the differences between targeted cells and non-targeted cells. It is of considerable interest due to its potential application in the biomedical field of various specific detections.
National Key Laboratory of Nano/Micro Fabrication Technology, Institute of Micro and Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, China. leon_chen@sjtu.edu.cn
Lei
L
Chen
Chen-Chen
CC
Bao
Hao
H
Yang
Ding
D
Li
Chong
C
Lei
Tao
T
Wang
Heng-Yao
HY
Hu
Meng
M
He
Yong
Y
Zhou
Da-Xiang
DX
Cui
D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D004867:Equipment Design; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D008280:Magnetics / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009842:Oligopeptides / Q000737:chemistry; D013274:Stomach Neoplasms / Q000175:diagnosis*
D005290:Ferric Compounds; D009842:Oligopeptides; C000499:ferric oxide; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan