Association of PPARG and LPIN1 gene polymorphisms with metabolic syndrome and type 2 diabetes.
OBJECTIVE
Lipin 1 is a recently discovered multifunctional protein involved in the metabolism of lipids, while PPARgamma is involved in adipocyte differentiation, and regulation of lipid metabolism. Up to now, LPIN1 and PPARG gene polymorphisms have been associated with type 2 diabetes, metabolic syndrome, and central obesity. In this study, we hypothesized that genetic variants within LPIN1 and PPARG genes were associated with traits of metabolic syndrome. Correlation between biochemical parameters (including but not limited to, glucose, HbA1c, insulin levels, HDL and LDL cholesterol, triglycerides, serum proteins, liver enzymes) and frequency of polymorphisms in LPIN1 (rs11693809 and rs2716610) and PPARG gene (rs10865710, rs3856806 and rs1801282), was tested in this study.


METHODS
The study included 70 patients diagnosed with metabolic syndrome and type 2 diabetes. Two polymorphisms of LPIN1 gene (rs11693809 and rs2716610), and three polymorphisms of PPARG gene (rs10865710, rs385806 and rs1801282) were analyzed by real time PCR and conventional PCR-RFLP methods.


RESULTS
Our analysis revealed correlation between insulin levels and rs11693809 LPIN1 polymorphism in diabetic patients. Also the results of this study showed an association of rs10865710 and rs385806 polymorphism of PPARG with HDL cholesterol and LDL plus total cholesterol levels, respectively.


CONCLUSIONS
These data reflect an association of analyzed PPARG and LPIN1 gene polymorphisms with values of insulin, HDL, LDL and total cholesterol witch indicates an important role of these genes in lipid metabolism and pathogenesis of type 2 diabetes and metabolic syndrome.
Department for Biochemistry and Clinical Analysis, Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. tamer.bego@gmail.com
Tamer
T
Bego
Tanja
T
Dujic
Barbara
B
Mlinar
Sabina
S
Semiz
Maja
M
Malenica
Besim
B
Prnjavorac
Barbara
B
Ostanek
Janja
J
Marc
Adlija
A
Causević
D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000235:genetics*; D006801:Humans; D007328:Insulin / Q000097:blood; D024821:Metabolic Syndrome / Q000150:complications / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics* / Q000502:physiology; D010711:Phosphatidate Phosphatase / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D007328:Insulin; D047495:PPAR gamma; C485999:LPIN1 protein, human; D010711:Phosphatidate Phosphatase