Identification of two blackleg resistance genes and fine mapping of one of these two genes in a Brassica napus canola cultivar 'Surpass 400'.
Blackleg resistant cultivars have been developed through conventional breeding methods and are successfully used globally to control this disease in canola production. To clone blackleg resistance genes and to understand the mechanism underlying the resistance, a blackleg resistant canola cultivar 'Surpass 400' was used to develop a gene mapping population. A previously reported high density genetic map was used to find a resistance gene region that corresponded to linkage group N10 in B. napus. Differential interactions between the resistant lines and a pathogen isolate were discovered with two resistance genes BLMR1 and BLMR2 identified through linkage analysis of five genome-specific molecular markers. BLMR1 provides resistance through the hypersensitive response that protects inoculated cotyledons from becoming infected, Unlike BLMR1, BLMR2 slows down the development of individual infection loci. BLMR1 and BLMR2 segregated independently in two large F(3)BC(2) populations. Fine mapping of BLMR1 was performed with 12 genome-specific molecular markers. The closest marker with a genetic distance of 0.13 cM to BLMR1 was identified, which lays a solid foundation for cloning BLMR1.
Department of Plant Science, University of Manitoba, Winnipeg, Canada.
Yunming
Y
Long
Zining
Z
Wang
Zudong
Z
Sun
Dilantha W G
DW
Fernando
Peter B E
PB
McVetty
Genyi
G
Li
D001203:Ascomycota / Q000472:pathogenicity*; D029688:Brassica napus / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology*; D002874:Chromosome Mapping / Q000379:methods*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D007113:Immunity, Innate / Q000235:genetics*; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology
D005819:Genetic Markers
Phytopathology. 2004 Jun;94(6):578-83
18943482
Plant J. 2001 Dec;28(5):545-53
11849594
Theor Appl Genet. 2008 Oct;117(6):895-904
18633592
Genetics. 2005 Dec;171(4):1977-88
16143600
Genome. 1997 Jun;40(3):294-301
18464830
Genome. 2008 Jan;51(1):64-72
18356940
Theor Appl Genet. 2005 Mar;110(5):969-79
15798929
Theor Appl Genet. 2007 May;114(8):1305-17
17426959