Histopathological characterization of primary cutaneous melanoma using infrared microimaging: a proof-of-concept study.
BACKGROUND
The diagnosis of malignant melanoma is based upon the histological evaluation of the lesion. As such, the morphological interpretation relies on the expertise of a dermatopathologist. Infrared microimaging is emerging as a new powerful tool to investigate tissue biochemistry. Infrared spectra probe the biochemical constitution of the sample and are real tissue-specific spectroscopic fingerprints.


OBJECTIVE
To assess the potential of infrared microimaging to aid in the analysis of tissue sections from primary cutaneous melanomas.


METHODS
Ten samples of melanoma sections from the main histological subtypes were investigated using infrared microimaging combined with multivariate statistical analyses.


RESULTS
This methodology yielded highly contrasted colour-coded images that permitted to highlight tissue architecture without any staining. It was possible to discriminate tumour areas from normal epidermis automatically, and intratumoral heterogeneity as revealed by our approach was correlated with the aggressiveness of the tumour.


CONCLUSIONS
This proof-of-concept study shows that infrared microimaging could help in the diagnosis of primary cutaneous melanoma.
Unité MéDIAN, CNRS UMR 6237 MEDyC, Université de Reims-Champagne Ardenne, IFR 53, 51 rue Cognacq-Jay, 51096 Reims Cedex, France.
E
E
Ly
N
N
Cardot-Leccia
J-P
JP
Ortonne
M
M
Benchetrit
J-F
JF
Michiels
M
M
Manfait
O
O
Piot
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007259:Infrared Rays; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000473:pathology; D008875:Middle Aged; D015999:Multivariate Analysis; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*