Human MxA protein inhibits the replication of classical swine fever virus.
Classical swine fever virus (CSFV) has a spherical enveloped particle with a single stranded RNA genome, the virus belonging to a pestivirus of the family Flaviviridae is the causative agent of an acute contagious disease classical swine fever (CSF). The interferon-induced MxA protein has been widely shown to inhibit the life cycle of certain RNA viruses as members of the Bunyaviridae family and others. Interestingly, it has been reported that expression of MxA in infected cells was blocked by CSFV and whether MxA has an inhibitory effect against CSFV remains unknown to date until present. Here, we report that CSFV replicated poorly in cells stably transfected with human MxA. The proliferation of progeny virus in both PK-15 cell lines and swine fetal fibroblasts (PEF) continuously expressing MxA was shown significantly inhibited as measured by virus titration, indirect immune fluorescence assay and real-time PCR.
Department of Animal Biotechnology, Jilin Provincial Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, Jilin, PR China. zhao008@hotmail.com
Yicheng
Y
Zhao
Daxin
D
Pang
Tiedong
T
Wang
Xin
X
Yang
Rong
R
Wu
Linzhu
L
Ren
Ting
T
Yuan
Yongye
Y
Huang
Hongsheng
H
Ouyang
D000818:Animals; D002460:Cell Line; D006691:Classical Swine Fever / Q000821:virology; D006692:Classical Swine Fever Virus / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D018628:Gene Dosage; D023061:Gene Order; D006801:Humans; D064547:Myxovirus Resistance Proteins; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism*; D015183:Restriction Mapping; D013552:Swine; D019562:Viral Load; D014779:Virus Replication / Q000502:physiology*
C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins