In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer.
Cellular damage caused by reactive oxygen species has been implicated in several diseases and hence antioxidants have significant importance in human health. Cold water, hot water and methanolic extract of Pleurotus squarrosulus were evaluated for antioxidant activity against hydroxyl radical, DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical, superoxide radical, nitric oxide (NO) scavenging, reducing power, ferrous ion chelating ability and beta-carotenellinoleic acid assay. Total phenol, flavonoid, beta-carotene and lycopene content were also determined. Hot water extract showed significant antioxidant activity in all the test systems. Hydroxyl radical scavenging activity of all the extracts has been significant compared to positive control. Hot water extract has been found to have higher phenolic, total flavonoid, beta-carotene and lycopene content than cold water and methanolic extract of the mushroom. Results of this study showed that, hot water extract has maximum antioxidant property and may be utilized as a promising source of therapeutics.
Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, India.
Jaita
J
Pal
Sourav
S
Ganguly
Khandakar Saifa
KS
Tahsin
Krishnendu
K
Acharya
D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002338:Carotenoids / Q000032:analysis; D005296:Ferrous Compounds / Q000494:pharmacology; D005419:Flavonoids; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D007202:Indicators and Reagents / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation; D000077276:Lycopene; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010851:Picrates / Q000494:pharmacology; D020076:Pleurotus / Q000737:chemistry* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D019207:beta Carotene / Q000032:analysis
D000975:Antioxidants; D001713:Biphenyl Compounds; D005296:Ferrous Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D005609:Free Radicals; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D010636:Phenols; D010851:Picrates; D017382:Reactive Oxygen Species; D019207:beta Carotene; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D002338:Carotenoids; C004931:1,1-diphenyl-2-picrylhydrazyl; D000077276:Lycopene