Efficient chimeric promoters derived from full-length and sub-genomic transcript promoters of Figwort mosaic virus (FMV).
Addition of multiple repeats of the FS3 upstream activation sequence (FS3-UAS, -270 to -60) intra-molecularly to the TATA containing core-domain of the FS3 (-151 to +31) promoter resulted in 2-3-folds enhanced promoter activity. The chimeric promoter, FS3-UAS-3X with maximum activity, showed 3.31 times stronger activity in root vascular tissue compared to FS3 promoter and could be used efficiently in translational research.
Department of Gene Function and Regulation, Institute of Life Sciences, Department of Biotechnology, Govt. of India, Nalco Square, Chandrasekherpur, Bhubaneswar 751 023, Orissa, India. rajivranjanbt@gmail.com
Rajiv
R
Ranjan
Sunita
S
Patro
Sangeeta
S
Kumari
Deepak
D
Kumar
Nrisingha
N
Dey
Indu B
IB
Maiti
D017796:Caulimovirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*