Intranasal co-administration with the mouse zona pellucida 3 expressing construct and its coding protein induces contraception in mice.
The zona pellucida 3 (ZP3), an autoantigen, once used to develop contraceptive vaccine has been faced a safety issue. Avoiding its pathogenic T cell activation, we intranasally co-delivered the mZP3 DNA- and protein-based vaccines in mice and observed that a higher level of sIgA and IgG antibodies in vaginal washes, bronchoalveolar lavages and serum and yielded a lower level of fertility and mean litter size. Importantly, histological analysis showed that normal follicular developments of the infertile mice were not disrupted in the co-delivered group. Thus, the intranasal co-delivery may present a safe strategy for the development of contraceptive vaccine.
Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, PR China.
Ailian
A
Zhang
Jinyao
J
Li
Gan
G
Zhao
Shuang
S
Geng
Shuzhen
S
Zhuang
Bin
B
Wang
Fuchun
F
Zhang
D000281:Administration, Intranasal; D000818:Animals; D018893:Bronchoalveolar Lavage; D003267:Contraception; D016207:Cytokines / Q000276:immunology; D004527:Egg Proteins / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D005298:Fertility; D019084:Fluorescent Antibody Technique, Indirect; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000276:immunology; D010053:Ovary / Q000276:immunology / Q000473:pathology; D011956:Receptors, Cell Surface / Q000008:administration & dosage / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D014611:Vaccination; D022642:Vaccines, Contraceptive / Q000008:administration & dosage / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D044364:Vaginal Douching; D000072216:Zona Pellucida Glycoproteins
D016207:Cytokines; D004527:Egg Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D022642:Vaccines, Contraceptive; D019444:Vaccines, DNA; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse