Role of tetraspanin CD151-α3/α6 integrin complex: Implication in angiogenesis CD151-integrin complex in angiogenesis.
Tetraspanin CD151 mainly associates with laminin-binding integrins and forms CD151-integrin complex. We previously reported that CD151 could be a potential target for angiogenesis, but the mechanisms involved are still unclear. This study investigated the role of CD151-integrin complex in angiogenesis and the signaling mechanisms involved. Here we showed that CD151 and CD151-AAA mutant were both well expressed at the protein level. CD151 gene transfer promoted angiogenesis and improved skin temperature of the lateral ischemic hindlimb, whereas CD151-AAA mutant abrogated the increase in capillary density and skin temperature. Further, CD151-AAA mutant failed to activate the FAK, ERK, PI3K/Akt/eNOS, and Rac1/Cdc42 signaling pathways. Moreover, CD151-AAA mutant was unavailable to promote bovine aortic endothelial cells (BAECs) proliferation and migration, in contrast to the effects of CD151. The results suggested that formation of CD151-integrin complex was likely to be a prerequisite for CD151-induced angiogenesis and signaling pathways.
Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Ave., Wuhan 430030, China.
Wei-Feng
WF
Liu
Hou-Juan
HJ
Zuo
Bo-lan
BL
Chai
Dan
D
Peng
Yu-Jie
YJ
Fei
Jing-Yang
JY
Lin
Xiao-Hui
XH
Yu
Dao-Wen
DW
Wang
Zheng-Xiang
ZX
Liu
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002196:Capillaries / Q000378:metabolism; D002417:Cattle; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D000229:Dependovirus / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D039422:Integrin alpha3 / Q000378:metabolism*; D039503:Integrin alpha6 / Q000378:metabolism*; D008297:Male; D009154:Mutation; D018919:Neovascularization, Physiologic; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D012881:Skin Temperature; D060185:Tetraspanin 24; D014945:Wound Healing
D015703:Antigens, CD; C554715:CD151 protein, human; D039422:Integrin alpha3; D039503:Integrin alpha6; D060185:Tetraspanin 24