Molecular characterization of high plant species using PCR with primers designed from consensus branch point signal sequences.
A novel method is introduced for producing molecular markers in plants using single 15- to 18-mer PCR primers designed from the short conserved consensus branch point signal sequences and standard agarose gel electrophoresis. This method was tested on cultivated peanut and verified to give good fingerprinting results in other plant species (mango, banana, and longan). These single primers, designed from relatively conserved branch point signal sequences within gene introns, should be universal across other plant species. The method is rapid, simple, and efficient, and it requires no sequence information of the plant genome of interest. It could be used in conjunction with, or as a substitute for, conventional RAPD or ISSR techniques for applications including genetic diversity analysis, phylogenetic tree construction, and quantitative trait locus mapping. This technique provides a new way to develop molecular markers for assessing genetic diversity of germplasm in diverse species based on conserved branch point signal sequences.
Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China. xfq2002@126.com
Faqian
F
Xiong
Jing
J
Jiang
Zhuqiang
Z
Han
Ruichun
R
Zhong
Liangqiong
L
He
Weijian
W
Zhuang
Ronghua
R
Tang
D010367:Arachis / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D005838:Genotype; D031022:Mangifera / Q000235:genetics; D028521:Musa / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D021382:Protein Sorting Signals; D022821:RNA Splice Sites / Q000235:genetics*; D032127:Sapindus / Q000235:genetics; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D021382:Protein Sorting Signals; D022821:RNA Splice Sites