Bro1 binding to Snf7 regulates ESCRT-III membrane scission activity in yeast.
Endosomal sorting complexes required for transport (ESCRTs) promote the invagination of vesicles into the lumen of endosomes, the budding of enveloped viruses, and the separation of cells during cytokinesis. These processes share a topologically similar membrane scission event facilitated by ESCRT-III assembly at the cytosolic surface of the membrane. The Snf7 subunit of ESCRT-III in yeast binds directly to an auxiliary protein, Bro1. Like ESCRT-III, Bro1 is required for the formation of intralumenal vesicles at endosomes, but its role in membrane scission is unknown. We show that overexpression of Bro1 or its N-terminal Bro1 domain that binds Snf7 enhances the stability of ESCRT-III by inhibiting Vps4-mediated disassembly in vivo and in vitro. This stabilization effect correlates with a reduced frequency in the detachment of intralumenal vesicles as observed by electron tomography, implicating Bro1 as a regulator of ESCRT-III disassembly and membrane scission activity.
Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.
Megan
M
Wemmer
Ishara
I
Azmi
Matthew
M
West
Brian
B
Davies
David
D
Katzmann
Greg
G
Odorizzi
D000251:Adenosine Triphosphatases / Q000378:metabolism; D020816:Amino Acid Motifs; D001665:Binding Sites; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000378:metabolism*; D057146:Multivesicular Bodies / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D054875:Ubiquitination
C480237:Bro1 protein, S cerevisiae; D056827:Endosomal Sorting Complexes Required for Transport; C083890:SNF7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C107528:VPS4 protein, S cerevisiae; D000251:Adenosine Triphosphatases
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