Effects of 2-aminoindan-2-phosphonic acid treatment on the accumulation of salidroside and four phenylethanoid glycosides in suspension cell culture of Cistanche deserticola.
2-Aminoindan-2-phosphonic acid (AIP), a specific competitive phenylalanine ammonia lyase (PAL) inhibitor was applied to a suspension cell culture of Cistanche deserticola. The effects of AIP treatment on cell growth, PAL activity, contents and yields of total phenolic compound, salidroside and four phenylethanoid glycosides (PheGs) are investigated. The results demonstrated that, 0.5 and 2.0 μM AIP treatments had similar effects on the measurements investigated in this study. AIP treatment resulted in significant decreases in PAL activity, total phenolic compounds content, and PheGs content. Linear regression analysis showed that PAL activity had a high correlation coefficient with the total phenolic compound content and the four PheGs contents. Total PAL activity-time area under curve (AUC) had a high correlation coefficient with the total phenolic compound yield and the yields of five tested compounds in untreated cell samples. In AIP-treated cells, total PAL activity-time AUC retained a high correlation with the total phenolic compound yield and the yields of three tested compounds, echinacoside, acteoside, and tubuloside A, but not salidroside and cistanoside A. The difference could be caused by the different biosynthetic origins of each of the tested compounds. These results demonstrate the important role of PAL in the biosynthesis of PheGs in the suspension cell culture of C. deserticola.
College of Life Science and Natural Resources, Dong-A University, Busan 604-714, Republic of Korea.
Gao Sheng
GS
Hu
Yeon Jae
YJ
Hur
Jing Ming
JM
Jia
Jai Heon
JH
Lee
Young Soo
YS
Chung
Young Byung
YB
Yi
Dae Jin
DJ
Yun
Soon Ki
SK
Park
Doh Hoon
DH
Kim
D002396:Catechols; D002851:Chromatography, High Pressure Liquid; D031671:Cistanche / Q000187:drug effects* / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D007189:Indans; D063065:Organophosphonates / Q000494:pharmacology*; D010636:Phenols / Q000378:metabolism*; D059808:Polyphenols
D002396:Catechols; D005960:Glucosides; D006027:Glycosides; D007189:Indans; D063065:Organophosphonates; D010636:Phenols; C411146:2-aminoindan-2-phosphonic acid; C555977:cistanoside A; C058956:acteoside; D059808:Polyphenols; C060297:echinacoside; C009172:rhodioloside
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