[Oxidative biotransformation of thioanisole by Rhodococcus rhodochrous IEGM 66 cells].
Comparative study of sulfoxidation activity of free and immobilized Rhodococcus rhodochrous IEGM 66 cells was performed. Free Rhodococcus cells (in the presence of 0.1 vol % n-hexadecane) displayed maximal oxidative activity towards thioanisole (0.5 g/l), a prochiral organic sulfide, added after 48-h cultivation of bacterial cells. Higher sulfide concentrations inhibited sulfoxidation activity of Rhodococcus. Use of immobilized cells allowed the 2-day preparatory stage to be omitted and a complete thioanisole bioconversion to be achieved in 24 h in the case that biocatalyst and 0.5 g/l thioanisole were added simultaneously. The biocatalyst immobilized on gel provides for complete thioanisole transformation into (S)-thioanisole sulfoxide (optical purity of 82.1%) at high (1.0-1.5 g/l) concentrations of sulfide substrate.
A A
AA
El'kin
V V
VV
Grishko
I B
IB
Ivshina
D000473:Alkanes; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D010084:Oxidation-Reduction; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*
D000473:Alkanes; D013440:Sulfides; C093850:methylphenylsulfide; C007932:n-hexadecane