Electrogenerated chemiluminescence detection in paper-based microfluidic sensors.
This paper describes the first approach at combining paper microfluidics with electrochemiluminescent (ECL) detection. Inkjet printing is used to produce paper microfluidic substrates which are combined with screen-printed electrodes (SPEs) to create simple, cheap, disposable sensors which can be read without a traditional photodetector. The sensing mechanism is based on the orange luminescence due to the ECL reaction of tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) with certain analytes. Using a conventional photodetector, 2-(dibutylamino)ethanol (DBAE) and nicotinamide adenine dinucleotide (NADH) could be detected to levels of 0.9 μM and 72 μM, respectively. Significantly, a mobile camera phone can also be used to detect the luminescence from the sensors. By analyzing the red pixel intensity in digital images of the ECL emission, a calibration curve was constructed demonstrating that DBAE could be detected to levels of 250 μM using the phone.
Department of Chemistry, La Trobe University, Victoria, Australia.
Jacqui L
JL
Delaney
Conor F
CF
Hogan
Junfei
J
Tian
Wei
W
Shen
D040421:Cell Phone; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004209:Disposable Equipment; D004563:Electrochemistry; D004566:Electrodes; D007281:Ink; D008163:Luminescent Measurements; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D009942:Organometallic Compounds / Q000737:chemistry; D010209:Paper; D011327:Printing
D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II)