Electrochemical detection of interaction between Thioflavin T and acetylcholinesterase.
The electrochemical oxidation of the benzothiazole dye Thioflavin T (ThT) was found to be modulated by its interaction with electric eel acetylcholinesterase (AChE). Modifications of AChE by trace amounts of small molecule inhibitors such as carbachol and paraoxon were detectable electrochemically using minimal reagents and with greater sensitivity than attainable through conventional fluorescence approaches. This property appears to be unique to ThT, since its closely related neutral derivative BTA-1 only interacts with AChE, but is not significantly affected by the presence of small molecule inhibitors.
Dept. of Physical and Environmental Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.
Vladimir
V
Dounin
Andrea
A
Constantinof
Holger
H
Schulze
Till T
TT
Bachmann
Kagan
K
Kerman
D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D052160:Benzothiazoles; D001665:Binding Sites; D002217:Carbachol / Q000737:chemistry / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D010084:Oxidation-Reduction; D010261:Paraoxon / Q000737:chemistry / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism*
D052160:Benzothiazoles; D002800:Cholinesterase Inhibitors; D013844:Thiazoles; C009462:thioflavin T; D002217:Carbachol; D000110:Acetylcholinesterase; D010261:Paraoxon