[Effects of acrolein exposure on the expression of Muc5ac in the airway of rats].
OBJECTIVE
To explore the changes of Muc5ac expression in a rat model of mucus hypersecretion induced by acrolein.


METHODS
The rat model of mucus hypersceretion was established by acrolein exposure. RT-PCR, ISH, immunohistochemistry and Western blot were employed to detect the mRNA and protein levels of Muc5ac in the rats airway.


RESULTS
The expression of Muc5ac was detected in trachea and lung at 3 weeks post exposure (wpe) and the expression level was enhanced at 6 wpe. ISH confirmed that Muc5ac was synthesized endogenously.


CONCLUSIONS
The pulmonary mucus hypersecretion is associated to the increase of intrinsic Muc5ac expression in the rat airway exposure to acrolein.
Department of Respiration, the First Affiliated Hospital of Yunnan Province, Kunming Medical College, Kunming 650000, China.
Bing
B
Yuan
Qi-qin
QQ
Dan
Shan
S
Zhao
Sheng-lan
SL
Wang
Bu-liang
BL
Meng
Yun-hui
YH
Zhang
D000171:Acrolein / Q000633:toxicity*; D000818:Animals; D004781:Environmental Exposure / Q000009:adverse effects; D008168:Lung / Q000378:metabolism*; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014132:Trachea / Q000378:metabolism
C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D012333:RNA, Messenger; D000171:Acrolein