[Investigation of differences between cold and hot nature of Mahuang decoction and Maxing Shigan decoction based on cold/hot plate differentiating assay].
OBJECTIVE
To investigate the objectivity and authenticity of the difference of the Cold and Hot nature between Mahuang decoction and Maxingshigan decoction based on the cold/hot plate differentiating assay.


METHODS
The measuring time and dosage of Mahuang decoction and Maxingshigan decoction were investigated before the compliance experiment. Meanwhile, the activities of adenosine triphosphatase were detected in order to probe into the mechanism of their action.


RESULTS
It was found that the drug dose should be 10 times amount to human dose (0.80 g x kg(-1) of Mahuang decoction and 2.26 g x kg(-1) of Maxing Shigan decoction) and the measuring time should be from 30 min to 60 min after administering. Compared with the control group, the remaining ratio of the mice treated with Mahuang decoction on the hot pad significantly decreased (P < 0.01). Compared with Mahuang decoction, the remaining ratio of the mice treated with Maxingshigan decoction on the hot pad significantly increased (P < 0.05). Compared with the control group, the Na(+) -K(+) -ATPase activity of liver tissue of the mice treated by Mahuang decoction significantly increased (P < 0.05), while the activity of Na(+) -K(+) -ATPase and Mg(2+) -Ca(2+) -ATPase of liver tissue of the mice treated by Maxingshigan decoction reduced significantly (P < 0.05).


CONCLUSIONS
This study showed that the differences of Cold and Hot nature of Mahuang decoction and Maxingshigan decoction were objectively existent from animal ethology, which was consistent with the traditional theroy of Chinese medicine. And the changes of ATPase activity of liver tissue might be the mechanism of drug action.
China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
Lei
L
Jia
Yanling
Y
Zhao
Xiaoyan
X
Xing
Jiabo
J
Wang
Wenjun
W
Zou
Ruisheng
R
Li
Hongbo
H
Yang
Danhong
D
Cheng
Xiaohe
X
Xiao
D000818:Animals; D003080:Cold Temperature; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029790:Ephedra sinica; D006358:Hot Temperature; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013697:Thermosensing / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; C530199:maxingshigan; D000254:Sodium-Potassium-Exchanging ATPase