Oleyl-chitosan nanoparticles based on a dual probe for optical/MR imaging in vivo.
Oleic acid-conjugated chitosan (oleyl-chitosan) is a powerful platform for encapsulating oleic acid-decorated iron oxide nanoparticles (ION), resulting in a good magnetic resonance imaging (MRI) probe. Oleyl-chitosan could self-assemble into core-shell structures in aqueous solution and provide the effective core compartment for loading ION. ION-loaded oleyl-chitosan nanoparticles showed good enhanced MRI sensitivity in a MR scanner. Cy5.5 dye was accessed to the oleyl-chitosan conjugate for near-infrared (NIR) in vivo optical imaging. After intravenous injection of ION-loaded Cy5.5-conjugated oleyl-chitosan (ION-Cy5.5-oleyl-chitosan) nanoparticles in tumor-bearing mice, both NIRF and MR imaging showed the detectable signal intensity and enhancement in tumor tissues via enhanced permeability and retention (EPR) effect. Tumor accumulation of the nanoparticles was confirmed through ex vivo fluorescence images and Prussian blue staining images in tumor tissues. It is concluded that ION-Cy5.5-oleyl-chitosan nanoparticle is highly an effective imaging probe for detecting tumor in vivo.
Department of Nuclear Medicine, Chonbuk National University Medical School and Hospital, Jeonju, Jeonbuk 561-712, Republic of Korea.
Chang-Moon
CM
Lee
Doorye
D
Jang
Jin
J
Kim
Su-Jin
SJ
Cheong
Eun-Mi
EM
Kim
Min-Hee
MH
Jeong
Sun-Hee
SH
Kim
Dong Wook
DW
Kim
Seok Tae
ST
Lim
Myung-Hee
MH
Sohn
Yong Yeon
YY
Jeong
Hwan-Jeong
HJ
Jeong
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D019301:Oleic Acid / Q000737:chemistry*; D010539:Permeability; D013194:Staining and Labeling; D023041:Xenograft Model Antitumor Assays
C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005290:Ferric Compounds; D005295:Ferrocyanides; C000499:ferric oxide; D019301:Oleic Acid; D048271:Chitosan; C000170:ferric ferrocyanide