Detection of colonic inflammation with Fourier transform infrared spectroscopy using a flexible silver halide fiber.
Persistent colonic inflammation increases risk for cancer, but mucosal appearance on conventional endoscopy correlates poorly with histology. Here we demonstrate the use of a flexible silver halide fiber to collect mid-infrared absorption spectra and an interval model to distinguish colitis from normal mucosa in dextran sulfate sodium treated mice. The spectral regime between 950 and 1800 cm(-1) was collected from excised colonic specimens and compared with histology. Our model identified 3 sub-ranges that optimize the classification results, and the performance for detecting inflammation resulted in a sensitivity, specificity, accuracy, and positive predictive value of 92%, 88%, 90%, and 88%, respectively.
Vinay K
VK
Katukuri
John
J
Hargrove
Sharon J
SJ
Miller
Kinan
K
Rahal
John Y
JY
Kao
Rolf
R
Wolters
Ellen M
EM
Zimmermann
Thomas D
TD
Wang
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