Effects of ferulic acid on hematopoietic cell recovery in whole-body gamma irradiated mice.
OBJECTIVE
The objective of this study was to investigate the mechanism for ferulic acid (FA)-induced radioprotection by evaluating the recovery of bone marrow cells and peripheral blood hematology.


METHODS
Balb/c mice were irradiated at a dose of 2.5 Gy using cobalt-60 gamma resources. Following irradiation, FA was administered intragastrically for seven consecutive days. Hematopoietic progenitor colony-forming cell assays were used to assess the reconstitution of bone marrow after radiation-induced myelosuppression. Cytokine levels were investigated using enzyme-linked immunosorbent assay and Western blot analysis.


RESULTS
The results demonstrated that FA treatment enhanced hematopoietic progenitor cell activity resulting in accelerated blood cell recovery. FA administration increased levels of granulocyte-colony stimulating factor (G-CSF) and erythropoietin.


CONCLUSIONS
These results suggest radioprotective efficacy by FA may be a result of early recovery of hematopoietic cells due to enhanced production of G-CSF and erythropoietin.
Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing, P. R. China.
Zeng-Chun
ZC
Ma
Qian
Q
Hong
Yu-Guang
YG
Wang
Hong-Ling
HL
Tan
Cheng-Rong
CR
Xiao
Qian-De
QD
Liang
Bei-Bei
BB
Lu
Yue
Y
Gao
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects; D003037:Cobalt Radioisotopes / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000378:metabolism; D005720:Gamma Rays; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D006405:Hematology / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects; D007202:Indicators and Reagents / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D014916:Whole-Body Irradiation / Q000379:methods*
D003037:Cobalt Radioisotopes; D003373:Coumaric Acids; D016207:Cytokines; D007202:Indicators and Reagents; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; C004999:ferulic acid