Chromatin accessibility pre-determines glucocorticoid receptor binding patterns.
Development, differentiation and response to environmental stimuli are characterized by sequential changes in cellular state initiated by the de novo binding of regulated transcriptional factors to their cognate genomic sites. The mechanism whereby a given regulatory factor selects a limited number of in vivo targets from a myriad of potential genomic binding sites is undetermined. Here we show that up to 95% of de novo genomic binding by the glucocorticoid receptor, a paradigmatic ligand-activated transcription factor, is targeted to preexisting foci of accessible chromatin. Factor binding invariably potentiates chromatin accessibility. Cell-selective glucocorticoid receptor occupancy patterns appear to be comprehensively predetermined by cell-specific differences in baseline chromatin accessibility patterns, with secondary contributions from local sequence features. The results define a framework for understanding regulatory factor-genome interactions and provide a molecular basis for the tissue selectivity of steroid pharmaceuticals and other agents that intersect the living genome.
Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
Sam
S
John
Peter J
PJ
Sabo
Robert E
RE
Thurman
Myong-Hee
MH
Sung
Simon C
SC
Biddie
Thomas A
TA
Johnson
Gordon L
GL
Hager
John A
JA
Stamatoyannopoulos
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000494:pharmacology; D051379:Mice; D009928:Organ Specificity; D011485:Protein Binding / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism
D002843:Chromatin; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors; D003850:Deoxyribonuclease I
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