Isolation and characterizations of clarithromycin-resistant Mycobacterium avium clinical isolates.
Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolate the clarithromycin-resistant strains from clinical isolates of M. avium and characterize them, we isolated the clarithromycin-resistant strains from clinical isolates of M. avium using reverse hybridization assay (RHA) and broth microdilution test (BMT). Three clarithromycin-resistant isolates with high level of MICs were found from 274 clinical isolates by BMT. Two of three resistant strains were also found by RHA, which revealed point mutations in the domain V region of the 23S rRNA. We report here clarithromycin-resistant clinical isolates of M. avium with the different characteristics from those of the resistant strains reported from earlier studies.
Korean Institute of Tuberculosis, Seoul, Korea.
Seung Heon
SH
Lee
Young Kil
YK
Park
Chang Ki
CK
Kim
Hee Jin
HJ
Kim
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D004252:DNA Mutational Analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D015269:Mycobacterium avium Complex / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000235:genetics; D056910:Republic of Korea
D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin
Antimicrob Agents Chemother. 2003 Oct;47(10):3053-60
14506008
JAMA. 1982 Dec 10;248(22):2980-2
6982979
Antimicrob Agents Chemother. 2004 Jan;48(1):143-50
14693532
J Antibiot (Tokyo). 1988 Jan;41(1):142-4
3346187
Tuber Lung Dis. 2000;80(1):1-4
10897378
J Antimicrob Chemother. 1994 Jun;33(6):1091-116
7928804
Antimicrob Agents Chemother. 1994 Feb;38(2):381-4
8192472
Lepr Rev. 2001 Dec;72(4):441-8
11826480
J Antimicrob Chemother. 2002 Apr;49(4):679-81
11909844
Antimicrob Agents Chemother. 2006 Oct;50(10):3476-8
17005837
Antimicrob Agents Chemother. 1996 Jul;40(7):1676-81
8807061
J Antimicrob Chemother. 2005 Feb;55(2):170-7
15590712
Antimicrob Agents Chemother. 1995 Dec;39(12):2625-30
8592991
Antimicrob Agents Chemother. 2001 Jan;45(1):1-12
11120937