[Effect of processing on metabolism of amygdalin from bitter almond in rat].
OBJECTIVE
To study the influence of processing on metabolism of the main component of bitter almond-amygdalin in rat.


METHODS
The blood was collected at different times after amygdalin given by injection and oral, bitter almond and its processed production given by oral respectively, and then detected by both HPLC and HPLC-MS(n) methods after extraction pretreatment.


RESULTS
After injection, amygdalin was absorbed in prototype to blood rapidly, while the other three kinds of medicine given by oral were all not detected the prototype of amygdalin, but two metabolites were detected which were isomers of prunasin confirmed by mass spectrometry. The metabolic pathway of prunasin in processed bitter almond group was markedly different from the bitter almond group.


CONCLUSIONS
Processing has a significant effect on bitter almond metabolic processes in rats.
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China. fff885@126.com
Minfeng
M
Fang
Zhiling
Z
Fu
Qilin
Q
Wang
Shixiang
S
Wang
Chaoni
C
Xiao
Xiaohui
X
Zheng
D000678:Amygdalin / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D005260:Female; D008297:Male; D027861:Prunus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000678:Amygdalin