Dual transport properties of anion exchanger 1: the same transmembrane segment is involved in anion exchange and in a cation leak.
Previous results suggested that specific point mutations in human anion exchanger 1 (AE1) convert the electroneutral anion exchanger into a monovalent cation conductance. In the present study, the transport site for anion exchange and for the cation leak has been studied by cysteine scanning mutagenesis and sulfhydryl reagent chemistry. Moreover, the role of some highly conserved amino acids within members of the SLC4 family to which AE1 belongs has been assessed in AE1 transport properties. The results suggest that the same transport site within the AE1 spanning domain is involved in anion exchange or in cation transport. A functioning mechanism for this transport site is proposed according to transport properties of the different studied point mutations of AE1.
Institut de Biologie du Développement et Cancer, UMR6543 Université de Nice-Centre National de la Recherche Scientifique, 28 avenue Valrose, 06108 Nice cedex 2, France.
Damien
D
Barneaud-Rocca
Franck
F
Borgese
Hélène
H
Guizouarn
D019943:Amino Acid Substitution; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics / Q000378:metabolism*; D000838:Anions / Q000378:metabolism; D002414:Cations, Monovalent / Q000378:metabolism; D006801:Humans; D017136:Ion Transport / Q000502:physiology; D020125:Mutation, Missense; D017354:Point Mutation; D017434:Protein Structure, Tertiary; D014982:Xenopus laevis
D001457:Anion Exchange Protein 1, Erythrocyte; D000838:Anions; D002414:Cations, Monovalent
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