Generation of neutralising antibodies against porcine endogenous retroviruses (PERVs).
Antibodies neutralising porcine endogenous retroviruses (PERVs) were induced in different animal species by immunisation with the transmembrane envelope protein p15E. These antibodies recognised epitopes, designated E1, in the fusion peptide proximal region (FPPR) of p15E, and E2 in the membrane proximal external region (MPER). E2 is localised in a position similar to that of an epitope in the transmembrane envelope protein gp41 of the human immunodeficiency virus-1 (HIV-1), recognised by the monoclonal antibody 4E10 that is broadly neutralising. To detect neutralising antibodies specific for PERV, a novel assay was developed, which is based on quantification of provirus integration by real-time PCR. In addition, for the first time, highly effective neutralising antibodies were obtained by immunisation with the surface envelope protein of PERV. These data indicate that neutralising antibodies can be induced by immunisation with both envelope proteins.
Robert Koch Institute, Nordufer 20, D.13353 Berlin, Germany.
Danny
D
Kaulitz
Uwe
U
Fiebig
Magdalena
M
Eschricht
Christian
C
Wurzbacher
Reinhard
R
Kurth
Joachim
J
Denner
D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D020077:Endogenous Retroviruses / Q000276:immunology*; D018604:Epitope Mapping; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015700:HIV Envelope Protein gp41; D014760:Viral Fusion Proteins; C514787:gp41 protein, Human immunodeficiency virus 1