Selection of silk-binding peptides by phage display.
Peptides that bind to silkworm-derived silk fibroin fiber were selected from a phage-displayed random peptide library. The selected silk-binding peptides contained a consensus sequence QSWS which is important for silk-binding as confirmed by binding assays using phage and synthetic peptides. With further optimization, we anticipate that the silk-binding peptides will be useful for functionalization of silk for biomaterial applications.
Department of Biomedical Engineering, University of California, Davis, 451 Health Sciences Drive, Davis, CA 95616, USA.
Yoko
Y
Nomura
Vandana
V
Sharma
Asami
A
Yamamura
Yohei
Y
Yokobayashi
D020816:Amino Acid Motifs; D016384:Consensus Sequence; D005349:Fibroins / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism*; D011485:Protein Binding; D047011:Silk / Q000378:metabolism*
D019151:Peptide Library; D010455:Peptides; D047011:Silk; D005349:Fibroins
Biomaterials. 2003 Feb;24(3):401-16
12423595
J Am Chem Soc. 2005 Oct 12;127(40):13780-1
16201785
Int J Biol Macromol. 1999 Mar-Apr;24(2-3):145-50
10342758
Biotechnol Adv. 2010 Nov-Dec;28(6):849-58
20659548
J Mater Chem. 2009 Jun 23;19(36):6443-6450
20161439
Biomaterials. 2006 Dec;27(36):6064-82
16890988
J Biotechnol. 2000 Aug;74(2):95-103
11763506
Nat Mater. 2003 Sep;2(9):577-85
12951599