[Nuclear localization sequence of bovine gamma-interferon provides translocation of recombinant protein to yeast Pichia pastoris cell nucleus].
It is known that heterologous protein production in microorganisms has negative influence upon a host cell. In this regard, of particular interest is to know the fate of recombinant proteins in a cell. In this study, it was shown that NLS of bovine gamma-interferon functions in yeast Pichia pastoris cells. The absence of the C-terminal NLS of bovine gamma-interferon leads to cytoplasmic localization of the recombinant protein.
A E
AE
Gradoboeva
M V
MV
Padkina
D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics
D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma