Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells.
Dengue viruses infect cells by attaching to a surface receptor which remains unknown. The putative receptor molecules of dengue virus type 2 on the surface of mosquito (AP-61) and mammalian (LLC-MK2) cell lines were investigated. The immunochemical detection and structural analysis of carbohydrates demonstrated that the neutral glycosphingolipids, L-3 (GlcNAcβ1-3Manβ1-4Glcβ1-1'Cer) in AP-61 cells, and nLc(4) Cer (Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1'Cer) in LLC-MK2 cells were recognized by the virus. These findings strongly suggest that neutral glycosphingolipids share the key determinant for virus binding and that the β-GlcNAc residue may play an important role in dengue virus binding to the host cell surface.
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Thailand.
Sineewanlaya
S
Wichit
Akanitt
A
Jittmittraphap
Kazuya I P J
KI
Hidari
Butsaya
B
Thaisomboonsuk
Songsak
S
Petmitr
Sukathida
S
Ubol
Chie
C
Aoki
Saki
S
Itonori
Koichi
K
Morita
Takashi
T
Suzuki
Yasuo
Y
Suzuki
Wipawee
W
Jampangern
D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D009033:Culicidae / Q000378:metabolism* / Q000821:virology; D003715:Dengue / Q000378:metabolism* / Q000821:virology; D003716:Dengue Virus / Q000378:metabolism*; D006801:Humans; D007303:Insect Vectors / Q000378:metabolism* / Q000821:virology; D020014:K562 Cells; D008253:Macaca mulatta; D008322:Mammals / Q000378:metabolism* / Q000821:virology; D008969:Molecular Sequence Data; D020383:Neutral Glycosphingolipids / Q000737:chemistry / Q000378:metabolism*
D020383:Neutral Glycosphingolipids