[The expression of CD25 and FOXP3 in mouse asthma and the effect of dexamethasone].
OBJECTIVE
To detect the expression of CD25 and FOXP3 in mouse asthma model, and the effect of dexamethasone (DXM) on it.


METHODS
BALB/c mice were randomly divided into three groups which were of normal control group, asthmatic group and DXM treatment group. The pathological changes of lungs were detected by HE staining. The expressions of CD25 and FOXP3 from lungs were evaluated by Western blot and RT-PCR. The expression of FOXP3 from bone marrow of asthmatic group and DXM treatment group were detected by RT-PCR.


RESULTS
The expression of FOXP3 in asthmatic group and DXM treatment group was significantly higher than that of normal control group (P < 0.05), DXM could promote the expression of FOXP3 (P < 0.05). The expression level of CD25 in asthmatic group and DXM treatment group was higher than that of normal control group (P < 0.05), but no significant difference was observed between asthmatic and DXM treatment groups (P > 0.05). Expressed FOXP3 mRNA in bone marrow were detected in both asthmatic and DXM treatment groups, but higher expression level was observed in DXM treatment group.


CONCLUSIONS
The expression of CD25 and FOXP3 increased in mouse asthma model, DXM could promote the expression. Cells in bone marrow could express FOXP3, DXM maybe promote the expression of FOXP3.
Gerontology Department, the Second People's Hospital of Chengdu, Chengdu 610031, China.
Xiang
X
Ma
Zong-an
ZA
Liang
Hui
H
Mao
Ya
Y
Liu
Mao-yun
MY
Wang
D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D003907:Dexamethasone / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation
D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D003907:Dexamethasone