Intrinsic optical signal imaging of glucose-stimulated insulin secreting β-cells.
Simultaneous monitoring of many functioning β-cells is essential for understanding β-cell dysfunction as an early event in the progression to diabetes. Intrinsic optical signal (IOS) imaging has been shown to allow high resolution detection of stimulus-evoked physiological responses in the retina and other neural tissues. In this paper, we demonstrate the feasibility of using IOS imaging for functional examination of insulin secreting INS-1 cells, a popular model for investigating diabetes associated β-cell dysfunction. Our experiments indicate that IOS imaging permits simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid IOS image sequences revealed transient optical responses that had time courses tightly correlated with the glucose stimulation.
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Yi-Chao
YC
Li
Wan-Xing
WX
Cui
Xu-Jing
XJ
Wang
Franklin
F
Amthor
Rong-Wen
RW
Lu
Anthony
A
Thompson
Xin-Cheng
XC
Yao
D000818:Animals; D002460:Cell Line; D003952:Diagnostic Imaging; D005947:Glucose / Q000494:pharmacology; D007259:Infrared Rays; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D055620:Optical Phenomena; D051381:Rats; D015398:Signal Transduction
D007328:Insulin; D005947:Glucose
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