Yeast H2A.Z, FACT complex and RSC regulate transcription of tRNA gene through differential dynamics of flanking nucleosomes.
FACT complex is involved in elongation and ensures fidelity in the initiation step of transcription by RNA polymerase (pol) II. Histone variant H2A.Z is found in nucleosomes at the 5'-end of many genes. We report here H2A.Z-chaperone activity of the yeast FACT complex on the short, nucleosome-free, non-coding, pol III-transcribed yeast tRNA genes. On a prototype gene, yeast SUP4, chromatin remodeler RSC and FACT regulate its transcription through novel mechanisms, wherein the two gene-flanking nucleosomes containing H2A.Z, play different roles. Nhp6, which ensures transcription fidelity and helps load yFACT onto the gene flanking nucleosomes, has inhibitory role. RSC maintains a nucleosome abutting the gene terminator downstream, which results in reduced transcription rate in active state while H2A.Z probably helps RSC in keeping the gene nucleosome-free and serves as stress-sensor. All these factors maintain an epigenetic state which allows the gene to return quickly from repressed to active state and tones down the expression from the active SUP4 gene, required probably to maintain the balance in cellular tRNA pool.
Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad 500007, India.
Sahasransu
S
Mahapatra
Pooran S
PS
Dewari
Anubhav
A
Bhardwaj
Purnima
P
Bhargava
D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D024001:HMGN Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism / Q000502:physiology*; D018832:Molecular Chaperones / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors / Q000502:physiology*
D002843:Chromatin; D004268:DNA-Binding Proteins; D024001:HMGN Proteins; D006657:Histones; C493530:Htz1 protein, S cerevisiae; D018832:Molecular Chaperones; C086471:NHP6A protein, S cerevisiae; D009707:Nucleosomes; C106768:POB3 protein, S cerevisiae; C103337:RSC complex, S cerevisiae; C071238:SPT16 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; D012343:RNA, Transfer; D012319:RNA Polymerase II
Biochem Cell Biol. 2006 Aug;84(4):477-89
16936821
Mol Cell. 2007 Feb 9;25(3):357-68
17289584
Mol Cell Biol. 2003 Nov;23(22):8323-33
14585989
EMBO J. 2001 Jul 2;20(13):3506-17
11432837
Mol Cell. 2001 Feb;7(2):309-18
11239460
Mol Cell. 2009 Aug 14;35(3):377-83
19683500
Trends Genet. 2007 Dec;23(12):614-22
17977614
EMBO J. 2003 Sep 15;22(18):4738-47
12970186
Genes Dev. 2002 Apr 1;16(7):806-19
11937489
J Biol Chem. 1996 Sep 6;271(36):22189-95
8703032
Biochem Soc Trans. 2006 Dec;34(Pt 6):1082-7
17073756
Structure. 2008 Feb;16(2):166-79
18275809
Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18385-90
16344463
Cell. 1996 Jan 26;84(2):245-52
8565070
Cell. 1998 Jan 9;92(1):105-16
9489704
Genes Dev. 1998 Dec 15;12(24):3857-71
9869639
Mol Cell Biol. 1994 Apr;14(4):2391-403
8139543
Nature. 2007 Mar 29;446(7135):572-6
17392789
J Biol Chem. 2009 Aug 28;284(35):23461-71
19574230
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14695-700
14634212
Mol Cell Biol. 2004 May;24(9):3596-606
15082757
EMBO J. 2003 Jun 16;22(12):3175-87
12805231
J Biol Chem. 2003 Nov 14;278(46):45288-95
12952948
Curr Biol. 2006 Oct 10;16(19):R849-51
17027482
Cell. 2000 Oct 27;103(3):411-22
11081628
Mol Cell Biol. 2006 Apr;26(8):3018-28
16581777
FEBS J. 2005 Oct;272(20):5149-68
16218948
EMBO J. 2008 Jan 9;27(1):100-10
18059476
Nature. 1999 Jul 15;400(6741):284-8
10421373
Nat Genet. 2007 Oct;39(10):1235-44
17873876
Trends Biochem Sci. 2007 Feb;32(2):51-3
17174096
Cell. 2005 Oct 21;123(2):219-31
16239141
Mol Cell Biol. 2008 Apr;28(8):2598-607
18268003
Mol Cell Biol. 2006 Jul;26(13):4920-33
16782880
J Biol Chem. 2006 Mar 17;281(11):7445-51
16407207
Genome Biol. 2004;5(9):R62
15345046
Mol Cell Biol. 1992 Sep;12(9):4015-25
1508199
Epigenetics Chromatin. 2009 Mar 16;2(1):4
19291282
Genes Dev. 2005 Apr 15;19(8):942-54
15833917
Nat Struct Mol Biol. 2007 Nov;14(11):997-1007
17984962
Mol Cell Biol. 2001 May;21(9):3096-104
11287614
Mol Cell. 2009 May 14;34(4):405-15
19481521
Mol Cell. 2008 Apr 11;30(1):86-97
18406329
EMBO J. 2009 Apr 8;28(7):854-65
19214185
Science. 2003 Aug 22;301(5636):1096-9
12934008
Science. 2003 Aug 22;301(5636):1090-3
12934006
Mol Cell Biol. 1997 Jul;17(7):4178-90
9199353
J Biol Chem. 2006 Sep 29;281(39):29155-64
16816405
Mol Cell Biol. 2005 Jul;25(14):5812-22
15987999
PLoS Biol. 2005 Dec;3(12):e384
16248679
Cell. 2005 Oct 21;123(2):233-48
16239142
Mol Cell Biol. 2006 Dec;26(23):8710-21
17000768
Cell. 2010 Jan 8;140(1):136-47
20079334
Cell. 2009 May 1;137(3):445-58
19410542
Mol Cell Biol. 2004 May;24(9):3907-17
15082784
Science. 1997 Oct 24;278(5338):680-6
9381177
Nat Biotechnol. 1999 Oct;17(10):1030-2
10504710