Combination of HPLC chromatogram and hypoglycemic effect identifies isoflavones as the principal active fraction of Belamcanda chinensis leaf extract in diabetes treatment.
In previous study, we demonstrated the hypoglycemic effect of aqueous extract of Belamcanda chinensis leaves in rats. Here, we separated the aqueous extract of B. chinensis leaves and investigated the spectrum-effect relationships between HPLC chromatograms and hypoglycemic activities of different isolates from B. chinensis leaf extract. Sequential solvent extraction with petroleum ether, chloroform, acetic ester and n-butanol provided several isolates showing similar hypoglycemic activities, making it difficult to discriminate the active fractions. Stepwise elution through HP20 macroporous resin by water, 40% and 95% ethanol provided isolates with distinct hypoglycemic activities, representing a simple, rapid and efficient preparative separation method. Combination of HPLC chromatogram and pharmacological effect targeted a hypoglycemic activity-related region in HPLC chromatogram. Each peak in this region was analyzed by UV spectrum scan. Most of them were flavonoids in which tectoridin and swertisin were known flavonoids with anti-diabetic activities. In together, this work provides a general model of combination of HPLC chromatography and pharmacological effect to study the spectrum-effect relationships of aqueous extract from B. chinensis leaves, which can be used to find principle components of B. chinensis on pharmacological activity.
School of Life Science, Beijing Institute of Technology, Beijing, PR China.
Yan
Y
Chen
Chong-Ming
CM
Wu
Rong-Ji
RJ
Dai
Liang
L
Li
Yu-Hong
YH
Yu
Yan
Y
Li
Wei-Wei
WW
Meng
Liang
L
Zhang
Yongqian
Y
Zhang
Yu-Lin
YL
Deng
D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D007003:Hypoglycemia / Q000097:blood / Q000188:drug therapy; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000494:pharmacology*; D027621:Iridaceae / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D007529:Isoflavones