Two-parameter angular light scatter collection for microfluidic flow cytometry by unique waveguide structures.
Waveguide numerical aperture restrictions and light-blocking elements are used to create a microfluidic cytometer with both illumination and two-parameter light scatter collection systems integrated on-chip. Good forward scatter coefficients of variation (9.7-18.3%) are achieved for polystyrene beads under a reasonably high flow rate (28 cm/s) using a greatly simplified optical system.
Department of Electrical and Computer Engineering, University of California, San Diego, 9500 Gilman Drive MC 0409, La Jolla, CA 92037, USA.
Jessica
J
Godin
Yu-Hwa
YH
Lo
Cytometry. 1990;11(2):223-30
2180652
Lab Chip. 2003 Feb;3(1):22-7
15100801
Lab Chip. 2003 Aug;3(3):187-92
15100772
Anal Chem. 1998 Dec 1;70(23):4974-84
21644679
Optoelectron Mater Devices. 2008 Nov 18;7135:71350M
20428483
Lab Chip. 2009 Jul 7;9(13):1942-50
19532970
Lab Chip. 2004 Aug;4(4):372-7
15269807
Anal Chem. 1999 Oct 1;71(19):4173-7
21662848
Biomed Microdevices. 2009 Dec;11(6):1223-31
19649710
Lab Chip. 2009 Apr 7;9(7):972-81
19294310