Supramolecular fishing for plasma membrane proteins using an ultrastable synthetic host-guest binding pair.
Membrane proteomics, the large-scale global analysis of membrane proteins, is often constrained by the efficiency of separating and extracting membrane proteins. Recent approaches involve conjugating membrane proteins with the small molecule biotin and using the receptor streptavidin to extract the labelled proteins. Despite the many advantages of this method, several shortcomings remain, including potential contamination by endogenously biotinylated molecules and interference by streptavidin during analytical stages. Here, we report a supramolecular fishing method for membrane proteins using the synthetic receptor-ligand pair cucurbit[7]uril-1-trimethylammoniomethylferrocene (CB[7]-AFc). CB[7]-conjugated beads selectively capture AFc-labelled proteins from heterogeneous protein mixtures, and AFc-labelling of cells results in the efficient capture of membrane proteins by these beads. The captured proteins can be recovered easily at room temperature by treatment with a strong competitor such as 1,1'-bis(trimethylammoniomethyl)ferrocene. This synthetic but biocompatible host-guest system may be a useful alternative to streptavidin-biotin for membrane proteomics as well as other biological and biotechnological applications.
National Creative Research Initiative Center for Smart Supramolecules, Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, Republic of Korea.
Don-Wook
DW
Lee
Kyeng Min
KM
Park
Mainak
M
Banerjee
Sang Hoon
SH
Ha
Taehoon
T
Lee
Kyungwon
K
Suh
Somak
S
Paul
Hyuntae
H
Jung
Jaeyoon
J
Kim
Narayanan
N
Selvapalam
Sung Ho
SH
Ryu
Kimoon
K
Kim
D000818:Animals; D001710:Biotin / Q000737:chemistry; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D015394:Molecular Structure; D011485:Protein Binding; D040901:Proteomics / Q000379:methods; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry
D006454:Hemoglobins; D008565:Membrane Proteins; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin
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