Targets of DNA-binding proteins in bacterial promoter regions present enhanced probabilities for spontaneous thermal openings.
We mapped promoter regions of double-stranded DNA with respect to the probabilities of appearance of relatively large bubble openings exclusively due to thermal fluctuations at physiological temperatures. We analyzed five well-studied promoter regions of procaryotic type and found a spatial correlation between the binding sites of transcription factors and the position of peaks in the probability pattern of large thermal openings. Other distinct peaks of the calculated patterns correlate with potential binding sites of DNA-binding proteins. These results suggest that a DNA molecule would more frequently expose the bases that participate in contacts with proteins, which would probably enhance the probability of the latter to reach their targets. It also stands for using this method as a means to analyze DNA sequences based on their intrinsic thermal properties.
IMBB, FORTH, PO Box 1385, 71110 Heraklion, Crete, Greece.
Angeliki
A
Apostolaki
George
G
Kalosakas
D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005798:Genes, Bacterial; D008962:Models, Theoretical; D056004:Molecular Dynamics Simulation; D009690:Nucleic Acid Conformation; D011336:Probability; D011401:Promoter Regions, Genetic; D011485:Protein Binding
D004268:DNA-Binding Proteins