Effect of copper on the expression of TGF-β in incubated chondrocytes of newborn pigs.
This experiment was conducted to measure the effect of copper supplementation on TGF-β gene expression in chondrocytes of newborn pigs. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. Total RNA was isolated from chondrocytes, and TGF-β cDNA was synthesized, amplified, and sequenced. The expression level of TGF-β was examined by reverse transcription polymerase chain reaction. The results showed that the sequence of the cloned TGF-β gene was 99.4% identical to that in GenBank. The expression of TGF-β increased in culture media added with final concentration of 15.6, 31.2, and 62.5 μmol/L copper. In this study, the optimal copper concentration and optimal culture time for the highest level of TGF-β expression were 31.2 μmol/L and 48 h, respectively.
College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, China.
Xiaoyan
X
Zhu
Jianguo
J
Wang
Guanghong
G
Xie
Haihua
H
Feng
Xiaobing
X
Li
Lei
L
Liu
Xiaoxu
X
Wang
Dongna
D
Li
Zhaoxi
Z
Liu
Jian
J
Qian
Zhe
Z
Wang
Guowen
G
Liu
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media; D017931:DNA Primers; D018076:DNA, Complementary; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D017931:DNA Primers; D018076:DNA, Complementary; D016212:Transforming Growth Factor beta; D003300:Copper