Five-substrate cocktail as a sensor array for measuring enzyme activity fingerprints of lipases and esterases.
Substrate arrays for measuring enzyme activity fingerprints can be conveniently formulated as cocktails designed such that the reaction products can be separated and quantified by analytical high-performance liquid chromatography (HPLC). Fingerprinting of lipases and esterases, an important class of microbial enzymes, is reported with a cocktail of only five substrates as a practical fingerprinting reagent. An unusually strong C4-esterase activity was thus revealed in a recently discovered microbial esterase.
Department of Chemistry and Biochemistry, University of Berne, Berne, Switzerland.
Noélie
N
Maillard
Peter
P
Babiak
Salahuddin
S
Syed
Rasomoy
R
Biswas
Luigi
L
Mandrich
Giuseppe
G
Manco
Jean-Louis
JL
Reymond
D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D004950:Esterases / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D010449:Peptide Mapping / Q000379:methods*; D013379:Substrate Specificity
D007202:Indicators and Reagents; D004950:Esterases; D008049:Lipase