Wnt5a regulates growth, patterning, and odontoblast differentiation of developing mouse tooth.
Wnt/β-catenin signaling is essential for tooth development beyond the bud stage, but little is known about the role of non-canonical Wnt signaling in odontogenesis. Here we compared the expression of Wnt5a, a representative of noncanonical Wnts, with that of Ror2, the Wnt5a receptor for non-canonical signaling, in the developing tooth, and analyzed tooth phenotype in Wnt5a mutants. Wnt5a-deficient mice exhibit retarded tooth development beginning from E16.5, leading to the formation of smaller and abnormally patterned teeth with a delayed odontoblast differentiation at birth. These defects are associated with upregulated Axin2 and Shh expression in the dental epithelium and reduced levels of cell proliferation in the dental epithelium and mesenchyme. Retarded tooth development and defective odontoblast differentiation were also observed in Ror2 mutant mice. Our results suggest that Wnt5a regulates growth, patterning, and odontoblast differentiation during odontogenesis, at least partially by modulating Wnt/β-catenin canonical signaling.
Department of Periodontology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Minkui
M
Lin
Lu
L
Li
Chao
C
Liu
Hongbing
H
Liu
Fenglei
F
He
Fuhua
F
Yan
Yanding
Y
Zhang
Yiping
Y
Chen
D000818:Animals; D060466:Axin Protein; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D051379:Mice; D018345:Mice, Knockout; D009804:Odontoblasts / Q000166:cytology* / Q000502:physiology*; D009805:Odontogenesis / Q000502:physiology*; D010641:Phenotype; D011247:Pregnancy; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D014070:Tooth / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D000071818:Wnt-5a Protein; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
D060466:Axin Protein; C111729:Axin2 protein, mouse; D003598:Cytoskeletal Proteins; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C438615:Wnt5a protein, mouse; D051176:beta Catenin; D057050:Receptor Tyrosine Kinase-like Orphan Receptors; C477010:Ror2 protein, mouse
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