Genetic, genomic, and molecular tools for studying the protoploid yeast, L. waltii.
Sequencing of the yeast Kluyveromyces waltii (recently renamed Lachancea waltii) provided evidence of a whole genome duplication event in the lineage leading to the well-studied Saccharomyces cerevisiae. While comparative genomic analyses of these yeasts have proven to be extremely instructive in modeling the loss or maintenance of gene duplicates, experimental tests of the ramifications following such genome alterations remain difficult. To transform L. waltii from an organism of the computational comparative genomic literature into an organism of the functional comparative genomic literature, we have developed genetic, molecular and genomic tools for working with L. waltii. In particular, we have characterized basic properties of L. waltii (growth, ploidy, molecular karyotype, mating type and the sexual cycle), developed transformation, cell cycle arrest and synchronization protocols, and have created centromeric and non-centromeric vectors as well as a genome browser for L. waltii. We hope that these tools will be used by the community to follow up on the ideas generated by sequence data and lead to a greater understanding of eukaryotic biology and genome evolution.
Department of Genome Sciences, University of Washington, Box 355065, Seattle, WA 98195, USA.
Sara C
SC
Di Rienzi
Kimberly C
KC
Lindstrom
Ragina
R
Lancaster
Lisa
L
Rolczynski
M K
MK
Raghuraman
Bonita J
BJ
Brewer
D005800:Genes, Fungal; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D016681:Genome, Fungal; D007716:Kluyveromyces / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D011995:Recombination, Genetic; D014170:Transformation, Genetic
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