Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus.
BACKGROUND
An effective method for obtaining resistant transgenic plants is to induce RNA silencing by expressing virus-derived dsRNA in plants and this method has been successfully implemented for the generation of different plant lines resistant to many plant viruses.


RESULTS
Inverted repeats of the partial Tobacco mosaic virus (TMV) movement protein (MP) gene and the partial Cucumber mosaic virus (CMV) replication protein (Rep) gene were introduced into the plant expression vector and the recombinant plasmids were transformed into Agrobacterium tumefaciens. Agrobacterium-mediated transformation was carried out and three transgenic tobacco lines (MP16-17-3, MP16-17-29 and MP16-17-58) immune to TMV infection and three transgenic tobacco lines (Rep15-1-1, Rep15-1-7 and Rep15-1-32) immune to CMV infection were obtained. Virus inoculation assays showed that the resistance of these transgenic plants could inherit and keep stable in T₄ progeny. The low temperature (15 °C did not influence the resistance of transgenic plants. There was no significant correlation between the resistance and the copy number of the transgene. CMV infection could not break the resistance to TMV in the transgenic tobacco plants expressing TMV hairpin MP RNA.


CONCLUSIONS
We have demonstrated that transgenic tobacco plants expressed partial TMV movement gene and partial CMV replicase gene in the form of an intermolecular intron-hairpin RNA exhibited complete resistance to TMV or CMV infection.
State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. China.
Qiong
Q
Hu
Yanbing
Y
Niu
Kai
K
Zhang
Yong
Y
Liu
Xueping
X
Zhou
D016960:Agrobacterium tumefaciens / Q000235:genetics; D017799:Cucumovirus / Q000235:genetics / Q000276:immunology*; D020868:Gene Silencing; D042822:Genomic Instability; D010935:Plant Diseases / Q000821:virology*; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000276:immunology*; D014170:Transformation, Genetic; D019076:Transgenes; D014764:Viral Proteins / Q000037:antagonists & inhibitors
D034741:RNA, Small Interfering; D014764:Viral Proteins
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