Pedobacter boryungensis sp. nov., isolated from soil.
A Gram-negative, non-sporulating, non-flagellated rod, designated BR-9(T), was isolated from soil collected on the Korean peninsula. Strain BR-9(T) grew optimally at pH 6.0-7.0, at 30 °C and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BR-9(T) belonged to the genus Pedobacter and clustered with Pedobacter insulae DS-139(T) and Pedobacter koreensis WPCB189(T). Strain BR-9(T) exhibited 98.2 and 97.5% 16S rRNA gene sequence similarity with P. insulae DS-139(T) and P. koreensis WPCB189(T), respectively, and <96.7% sequence similarity with the type strains of other species in the genus Pedobacter. Strain BR-9(T) contained MK-7 as the predominant menaquinone and iso-C(15:0) and summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) as the major fatty acids. The DNA G+C content of strain BR-9(T) was 38.5 mol%. DNA-DNA relatedness between strain BR-9(T) and P. insulae DS-139(T) and P. koreensis KCTC 12536(T) was 3.4-4.2%, which indicated that the isolate was genetically distinct from these type strains. Strain BR-9(T) was also distinguishable by differences in phenotypic properties. On the basis of the data presented, strain BR-9(T) is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter boryungensis sp. nov. is proposed. The type strain is BR-9(T) (=KCTC 23344(T) =CCUG 60024(T)).
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Yong-Taek
YT
Jung
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Soo-Young
SY
Lee
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Won-Chan
WC
Choi
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Tae-Kwang
TK
Oh
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D060485:Pedobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride