Bioengineering single crystal growth.
Biomineralization is a "bottom-up" synthesis process that results in the formation of inorganic/organic nanocomposites with unrivaled control over structure, superior mechanical properties, adaptive response, and the capability of self-repair. While de novo design of such highly optimized materials may still be out of reach, engineering of the biosynthetic machinery may offer an alternative route to design advanced materials. Herein, we present an approach using micro-contact-printed lectins for patterning sea urchin embryo primary mesenchyme cells (PMCs) in vitro. We demonstrate not only that PMCs cultured on these substrates show attachment to wheat germ agglutinin and concanavalin A patterns but, more importantly, that the deposition and elongation of calcite spicules occurs cooperatively by multiple cells and in alignment with the printed pattern. This allows us to control the placement and orientation of smooth, cylindrical calcite single crystals where the crystallographic c-direction is parallel to the cylinder axis and the underlying line pattern.
Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, United States.
Ching-Hsuan
CH
Wu
Alexander
A
Park
Derk
D
Joester
D000818:Animals; D057005:Bioengineering / Q000379:methods*; D002119:Calcium Carbonate / Q000737:chemistry*; D018929:Cell Culture Techniques; D003208:Concanavalin A / Q000737:chemistry; D008648:Mesoderm / Q000166:cytology; D055616:Microtechnology / Q000379:methods*; D011327:Printing; D012617:Sea Urchins / Q000166:cytology; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D002119:Calcium Carbonate