Typing of nitrogen-fixing Frankia strains by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry.
Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) was evaluated as a technique to characterize strains of the nitrogen-fixing actinomycete Frankia. MALDI-TOF MS reliably distinguished 37 isolates within the genus Frankia and assigned them to their respective host infection groups, i.e., the Alnus/Casuarina and the Elaeagnus host infection groups. The assignment of individual strains to sub-groups within the respective host infection groups was consistent with classification based on comparative sequence analysis of nifH gene fragments, confirming the usefulness of MALDI-TOF MS as a rapid and reliable tool for the characterization of Frankia strains.
Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666, USA. dh49@txstate.edu
Dittmar
D
Hahn
Babur
B
Mirza
Cinzia
C
Benagli
Guido
G
Vogel
Mauro
M
Tonolla
D015373:Bacterial Typing Techniques / Q000379:methods*; D040161:Frankia / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D058889:Molecular Typing; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*