An early diagnostic tool for diabetic neuropathy: conduction velocity distribution.
Diabetes is a metabolic disorder that affects much of the human population. As a secondary complication, diabetic neuropathy causes time-dependent damage to peripheral nerves. In this study, experimental diabetes was induced by streptozotocin (STZ; 50 mg/kg intraperitoneally) in rats. Diabetic animals were grouped into those with 2 or 4 weeks of diabetes, whereas a control group received only the STZ vehicle (0.1 M citrate). Sciatic nerves were dissected, and compound action potentials (CAPs) were recorded. Results deduced by conventional calculation carried less information when compared with conduction velocity distribution (CVD) obtained by a computer-based mathematical model. Using the conventional approach, statistically significant changes were first seen in the fourth week of diabetes, whereas results deduced by CVD measurement could be seen in the second week. Consequently, the CVD calculation provides more information for the early diagnosis of neuropathies compared with classical conduction velocity measurements.
Department of Biophysics, Selcuk University, Konya 42080, Meram/Konya, Turkey. tuncerseckin@gmail.com
Seckin
S
Tuncer
Nizamettin
N
Dalkilic
Haci Hasan
HH
Esen
Mustafa Cihat
MC
Avunduk
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D001703:Biophysics / Q000379:methods; D001786:Blood Glucose / Q000502:physiology; D001835:Body Weight / Q000187:drug effects; D003929:Diabetic Neuropathies / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000379:methods; D008297:Male; D008432:Mathematical Computing; D009046:Motor Neurons / Q000473:pathology; D009431:Neural Conduction / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology*; D012584:Sciatic Nerve / Q000187:drug effects / Q000503:physiopathology*; D013311:Streptozocin / Q000633:toxicity; D013997:Time Factors
D001786:Blood Glucose; D013311:Streptozocin