Highly sensitive detection of silybin based on adsorptive stripping analysis at single-sided heated screen-printed carbon electrodes modified with multi-walled carbon nanotubes with direct current heating.
A new disposable multi-walled carbon nanotubes modified single-sided heated screen-printed carbon electrode (MWNT/ss-HSPCE) was fabricated. The electrochemical behavior of silybin was investigated by cyclic voltammetry and the probable electrode reaction mechanism was proposed. A simple and cheap direct current heating supplier was used to heating the electrode for adsorptive accumulation of silybin. The square wave voltammetric stripping peak current of silybin at MWNT/ss-HSPCE with an elevated electrode temperature of 50°C only during accumulation step was dramatically improved compared with that at bare single-sided heated screen-printed carbon electrode (ss-HSPCE) without heating. This enhancement was mainly contributed to the combination of the advantages of multi-walled carbon nanotubes and electrically heated electrodes. Under optimum conditions, two detection linear ranges of silybin were from 1.0×10(-9) to 1.0×10(-7) M and 3.0×10(-7) to 1.0×10(-6) M. A detection limit of 5.0×10(-10) M could be obtained (S/N=3), which was more than two magnitudes lower than that at bare ss-HSPCE without heating. To the best of our knowledge, this was also at least two magnitudes lower than any others for electrochemical detection of silybin in the literature. Finally, the method was successfully applied to the determination of silybin in pharmaceutical tablets.
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350002, China.
Shao-Hua
SH
Wu
Fa-Hui
FH
Nie
Qi-Zhen
QZ
Chen
Jian-Jun
JJ
Sun
D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry*; D013607:Tablets / Q000737:chemistry; D013696:Temperature
D037742:Nanotubes, Carbon; D012838:Silymarin; D013607:Tablets; D000077385:Silybin; D002244:Carbon