Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE).
Salt stress is a major abiotic stress that limits agricultural productivity in many regions of the world. To understand the molecular basis of the salt stress response in wheat (Triticum aestivum L.), a proteomic approach was used to identify the salt stress-responsive proteins in an elite Chinese wheat cultivar, Zhengmai 9023, which exhibits a high yield, superior gluten quality and better biotic resistance. Three-week-old seedlings were treated with NaCl of four different concentrations (1.0%, 1.5%, 2.0%, and 2.5%). The total proteins from the leaves of untreated and NaCl-treated plants were extracted and separated by two-dimensional difference gel electrophoresis (2D-DIGE). A total of 2358 protein spots were detected on the gels, among which 125 spots showed a significant change in protein abundance, and 83 differentially expressed spots were localised on preparative gels. Using Q-TOF mass spectrometry, 52 salt-responsive spots were identified, which were classified into six functional categories that included transport-associated proteins, detoxifying enzymes, ATP synthase, carbon metabolism, protein folding, and proteins with unknown biological functions. Of the 52 differentially expressed proteins, 26 were up-regulated, 21 were down-regulated, and five spots showed multi-expression patterns. In particular, some important proteins for salt tolerance were found to be up-regulated in Zhengmai 9023 under salt stress, such as H(+)-ATPases, glutathione S-transferase, ferritin and triosephosphate isomerase.
College of Life Sciences, Capital Normal University, 100048 Beijing, China.
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Gao
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Yan
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Li
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Guo
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Hu
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Ma
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Yan
D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects; D010940:Plant Proteins / Q000032:analysis* / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry* / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects; D014908:Triticum / Q000737:chemistry* / Q000187:drug effects; D057825:Two-Dimensional Difference Gel Electrophoresis
D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride