Early dysregulation of the mitochondrial proteome in a mouse model of Alzheimer's disease.
Mitochondrial structural and functional alterations appear to play to an important role in the pathogenesis of Alzheimer's disease (AD). In the present study, we used a quantitative comparative proteomic profiling approach to analyze changes in the mitochondrial proteome in AD. A triple transgenic mouse model of AD (3xTg-AD) which harbors mutations in three human transgenes, APP(Swe), PS1(M146V) and Tau(P301L), was used in these experiments. Quantitative differences in the mitochondrial proteome between the cerebral cortices of 6-month-old male 3xTg-AD and non-transgenic mice were determined by using two-dimensional difference gel electrophoresis (2D-DIGE) and tandem mass spectrometry. We identified 23 different proteins whose expression levels differed significantly between triple transgenic and non-transgenic mitochondria. Both down-regulated and up-regulated mitochondrial proteins were observed in transgenic AD cortices. Proteins which were dysregulated in 3xTg-AD cortices functioned in a wide variety of metabolic pathways, including the citric acid cycle, oxidative phosphorylation, pyruvate metabolism, glycolysis, oxidative stress, fatty acid oxidation, ketone body metabolism, ion transport, apoptosis, and mitochondrial protein synthesis. These alterations in the mitochondrial proteome of the cerebral cortices of triple transgenic AD mice occurred before the development of significant amyloid plaque and neurofibrillary tangles, indicating that mitochondrial dysregulation is an early event in AD.
Department of Molecular and Cell Biology, The University of Texas at Dallas, Richardson, TX 75080, USA.
Jose L
JL
Chou
Deepa V
DV
Shenoy
Nicy
N
Thomas
Pankaj K
PK
Choudhary
Frank M
FM
Laferla
Steven R
SR
Goodman
Gail A M
GA
Breen
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis / Q000737:chemistry; D008297:Male; D051379:Mice / Q000378:metabolism; D008822:Mice, Transgenic; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism*; D008954:Models, Biological; D016874:Neurofibrillary Tangles / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013997:Time Factors; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods
D018969:Insulin-Like Growth Factor Binding Proteins; D024101:Mitochondrial Proteins; D020543:Proteome