Characterization of host genetic expression patterns in HIV-infected individuals with divergent disease progression.
The course of HIV-1 infection shows a variety of clinical phenotypes with an important involvement of host factors. We compare host gene expression patterns in CD3+ T cells from two of these phenotypes: long-term non-progressor patients (LTNP) and matched control patients with standard HIV disease progression. Array analysis revealed over-expression of 322 genes in progressors and 136 in LTNP. Up-regulated genes in progressors were mainly implicated in the regulation of DNA replication, cell cycle and DNA damage stimulus and mostly localized into cellular organelles. In contrast, most up-regulated genes in LTNP were located at the plasmatic membrane and involved in cytokine-cytokine receptor interaction, negative control of apoptosis or regulation of actin cytoskeleton. Regarding gene interactions, a higher number of viral genes interacting with cellular factors were seen in progressors. Our study offers new comparative insights related to disease status and can distinguish differentiated patterns of gene expression among clinical phenotypes.
Infectious Diseases Department, Hospital Carlos III, Madrid, Spain.
María
M
Salgado
Pedro
P
López-Romero
Sergio
S
Callejas
Mariola
M
López
Pablo
P
Labarga
Ana
A
Dopazo
Vincent
V
Soriano
Berta
B
Rodés
D000328:Adult; D017252:CD3 Complex / Q000032:analysis; D016022:Case-Control Studies; D018450:Disease Progression; D020869:Gene Expression Profiling; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology* / Q000473:pathology / Q000821:virology; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
D017252:CD3 Complex