Functionalized, biocompatible coating for superparamagnetic nanoparticles by controlled polymerization of a thioglycosidic monomer.
It is demonstrated that water-soluble, glucosylated poly(pentafluorostyrene) derivatives revealed favorable coating material properties for magnetic iron oxide nanoparticles. To prepare the coating material in high reproducibility and purity as well as in sufficient amounts, a new route of synthesis is established. The preparation and characterization of the glucosylated, tetrafluorostyryl monomer, by thiol-para-fluorine "click" reaction, and its polymerization, via nitroxide-mediated radical process, is presented in detail. In addition, the coating material and the resulting particle properties are investigated by means of XPS, DLS, TGA, TEM, and cryo-TEM as well as flow cytometry. The glycopolymer acts as an appropriate stabilizing agent for the superparamagnetic nanoparticles by the formation of an approximately 10 nm thick shell, as shown by the XPS analysis. Furthermore, the application of FITC-labeled glycopolymer yielded fluorescent, superparamagnetic nanoparticles, which can be used for monitoring cell-carbohydrate interactions, because these particles show no cytotoxicity toward 3T3 fibroblasts.
Laboratory of Organic and Macromolecular Chemistry (IOMC) and Jena Center for Soft Matter (JCSM), Friedrich-Schiller University Jena, Humboldtstr. 10, 07743 Jena, Germany.
Krzysztof
K
Babiuch
Ralf
R
Wyrwa
Kerstin
K
Wagner
Thomas
T
Seemann
Stephanie
S
Hoeppener
C Remzi
CR
Becer
Ralf
R
Linke
Michael
M
Gottschaldt
Jürgen
J
Weisser
Matthias
M
Schnabelrauch
Ulrich S
US
Schubert
D016475:3T3 Cells; D000818:Animals; D002241:Carbohydrates; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D005434:Flow Cytometry; D005453:Fluorescence; D008280:Magnetics; D051379:Mice; D058105:Polymerization; D013865:Thioglycosides / Q000737:chemistry*
D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D013865:Thioglycosides