Cancer field effects in normal tissues revealed by Raman spectroscopy.
It has been demonstrated that the presence of cancer results in detectable changes to uninvolved tissues, collectively termed cancer field effects (CFE). In this study, we directly assessed the ability of Raman microspectroscopy to detect CFE via in-vitro study of organotypic tissue rafts approximating human skin. Raman spectra were measured from both epidermis and dermis after transfer of the rafts to dishes containing adherent cultures of either normal human fibroblasts or fibrosarcoma (HT1080) cells. Principal components analyses allowed discrimination between the groups with 86% classification accuracy in the epidermis and 94% in the dermis. These results encourage further study to evaluate the Raman capacity for detecting CFE as a possible tool for noninvasive screening for tumor presence.
Chad A
CA
Lieber
Hubert E
HE
Nethercott
Mustafa H
MH
Kabeer
Appl Spectrosc. 2007 Sep;61(9):986-93
17910796
Int J Oncol. 2003 Sep;23(3):649-55
12888900
Appl Spectrosc. 2003 Nov;57(11):1363-7
14658149
Dis Markers. 2008;25(6):323-37
19208950
Lasers Surg Med. 2008 Sep;40(7):461-7
18727020
J Pathol. 2003 Jul;200(4):429-47
12845611
Anal Chem. 1995 Mar 1;67(5):777-83
7762814
Cancer Res. 2006 Mar 15;66(6):3317-22
16540686
Cancer Epidemiol Biomarkers Prev. 2005 Jul;14(7):1639-45
16030095
Photochem Photobiol. 2003 Nov;78(5):517-24
14653585
J Biomed Opt. 2006 Mar-Apr;11(2):021003
16674178
Gastroenterology. 2004 Apr;126(4):1071-81; discussion 948
15057746
Gastroenterology. 2008 Oct;135(4):1069-78
18722372