Photoactive Ru(II) -polypyridyl complexes that display sequence selectivity and high-affinity binding to duplex DNA through groove binding.
The duplex-DNA binding properties of a nonintercalating polypyridyl ruthenium(II) complex that incorporates a linear extended ligand with a catechol moiety has been probed with a variety of photo- and biophysical techniques. These studies reveal that the complex groove binds to DNA sequences biphasically, and displays binding constants equivalent to those of high-affinity metallointercalators. The complex also displays preferential binding to AT-rich sequences. Changes in the structure of the coordinated catechol ligand and the incorporation of intercalating ancillary ligands into the complex were found to modulate both the optical-binding response and binding parameters of the system, which indicates that the catechol moiety plays a crucial role in the observed enhancement to binding affinities.
Central Salt & Marine Chemicals Research Institute, Bhavnagar, 364002 Gujarat, India.
Amrita
A
Ghosh
Priyadip
P
Das
Martin R
MR
Gill
Prasenjit
P
Kar
Michael G
MG
Walker
Jim A
JA
Thomas
Amitava
A
Das
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D008024:Ligands; D049449:Luminescence; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010777:Photochemistry; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*
D007364:Intercalating Agents; D008024:Ligands; D009942:Organometallic Compounds; D011725:Pyridines; D012428:Ruthenium; D004247:DNA