[Effects of urokinase intervention on endotoxin-induced DIC in Wistar rat model].
OBJECTIVE
To study the effects of urokinase intervention on endotoxin-induced DIC in Wistar rats model.


METHODS
Wistar rats were randomly assigned into 4 groups: normal saline (NS) group, urokinase (UK) group, endotoxin (LPS) group and LPS+ UK group. These agents were given to the rats by the tail vein intravenous infusion, NS group was treated with NS 2.5 mL/h x 4 h; UK group with NS 2.5 mL/h, 1 h later UK 4 IU/(g x h) x 3 h; LPS group with LPS 3 mg/(kg x h) x 4 h; LPS+UK group with LPS 3 mg/(kg x h) firstly, 1 h later UK 4 IU/(g x h) ) < 3 h. After the intervention, the function of coagulation and fibrinolysis, the indicators of organ damage and microcirculation fibrin micro-clots were evaluated.


RESULTS
One hour after the infusion of 3 mg/(kg x h) of LPS, DIC signs began to appear, and became more apparent over time. After the intervention of urokinase, the values of clotting time (PT), activated partial thromboplastin time (APTT) were significantly shorter, but the platelet count (PLT), the amount of fibrinogen (FIB) changed little. Plasminogen activator inhibitor-1 (PAI-1) level decreased, while the D-dimer level increased. Serum creatinine (Cr), alanine aminotransferase (ALT) also decreased significantly. The biopsy of liver, kidney, and lung showed tissue damage became better, and the organ microcirculation fibrin micro-clots decreased significantly.


CONCLUSIONS
The concentration of 3 mg/(kg x h) endotoxin can successfully induce DIC model in Wistar rats. Urokinase could play a positive role to prevent the LPS-induced DIC.
Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Kai-feng
KF
Yuan
Xiao-mei
XM
Liao
Lei
L
Yu
Ting
T
Niu
Xian-liang
XL
Zhang
Wei-ping
WP
Liu
Dian-ying
DY
Liao
Wen-tong
WT
Meng
Xiao-fan
XF
Fei
D000818:Animals; D004195:Disease Models, Animal; D004211:Disseminated Intravascular Coagulation / Q000139:chemically induced* / Q000517:prevention & control*; D004731:Endotoxins; D005260:Female; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology / Q000627:therapeutic use*
D004731:Endotoxins; D014568:Urokinase-Type Plasminogen Activator