Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics.
Recent developments in reagent design can address problems in single cells that were not previously approachable. We have attempted to foresee what will become possible, and the sorts of biological problems that become tractable with these novel reagents. We have focused on the novel fluorescent proteins that allow convenient multiplexing, and provide for a time-dependent analysis of events in single cells. Methods for fluorescently labeling specific molecules, including endogenously expressed proteins and mRNA have progressed and are now commonly used in a variety of organisms. Finally, sensitive microscopic methods have become more routine practice. This article emphasizes that the time is right to coordinate these approaches for a new initiative on single cell imaging of biological molecules.
Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Bin
B
Wu
Kiryl D
KD
Piatkevich
Timothée
T
Lionnet
Robert H
RH
Singer
Vladislav V
VV
Verkhusha
D002467:Cell Nucleus / Q000737:chemistry; D003584:Cytological Techniques / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D015870:Gene Expression; D008164:Luminescent Proteins / Q000032:analysis* / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D059010:Single-Cell Analysis
D005456:Fluorescent Dyes; D008164:Luminescent Proteins
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