Protein microarray analysis of mammary epithelial cells from obese and nonobese women at high risk for breast cancer: feasibility data.
BACKGROUND
Obesity is a well-established risk factor for cancer, accounting for up to 20% of cancer deaths in women. Studies of women with breast cancer have shown obesity to be associated with an increased risk of dying from breast cancer and increased risk of developing distant metastasis. While previous studies have focused on differences in circulating hormone levels as a cause for increased breast cancer incidence in postmenopausal women, few studies have focused on potential differences in the protein expression patterns of mammary epithelial cells obtained from obese versus nonobese women.


METHODS
Protein expression was assessed by reverse-phase protein microarray in mammary epithelial cells from 31 random periareolar fine needle aspirations performed on 26 high-risk women.


RESULTS
In this pilot and exploratory study, vimentin (unadjusted P=0.028) expression was significantly different between obese and nonobese women.


CONCLUSIONS
Vimentin is integral both to adipocyte structure and function and to the epithelial-to-mesenchymal transition needed for cancer cell metastasis. Further research is needed to confirm this finding and determine the possible effects of the adipocyte microenvironment on the initiation and progression of breast cancer in high-risk women.


CONCLUSIONS
Differential protein expression patterns obtained from a future expanded study may serve to elaborate the underlying pathology of breast cancer initiation and progression in obese women and identify potential biomarkers of response to preventative interventions such as dietary changes and exercise.
Duke University Medical Center, Box 2628, Durham, NC 27710, USA.
Patrick G
PG
Pilie
Catherine
C
Ibarra-Drendall
Michelle M
MM
Troch
Gloria
G
Broadwater
William T
WT
Barry
Emanuel F
EF
Petricoin
Julia D
JD
Wulfkuhle
Lance A
LA
Liotta
Siya
S
Lem
Joseph C
JC
Baker
April
A
Stouder
Anne C
AC
Ford
Lee G
LG
Wilke
Carola M
CM
Zalles
Priya
P
Mehta
Jamila
J
Williams
Melanie
M
Shivraj
Zuowei
Z
Su
Joseph
J
Geradts
Dihua
D
Yu
Victoria L
VL
Seewaldt
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D044963:Biopsy, Fine-Needle / Q000379:methods; D015992:Body Mass Index; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D042361:Mammary Glands, Human / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D010865:Pilot Projects; D040081:Protein Array Analysis / Q000379:methods; D012189:Retrospective Studies; D012307:Risk Factors; D014746:Vimentin / Q000378:metabolism
D014408:Biomarkers, Tumor; D014746:Vimentin
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