Current state and perspectives of producing biodiesel-like compounds by biotechnology.
The global demand for crude oil is expected to continue to rise in future while simultaneously oil production is currently reaching its peak. Subsequently, rising oil prices and their negative impacts on economy, together with an increased environmental awareness of our society, directed the focus also on the biotechnological production of fuels. Although a wide variety of such fuels has been suggested, only the production of ethanol and biodiesel has reached a certain economic feasibility and volume, yet. This review focuses on the current state and perspectives of biotechnological production of biodiesel-like compounds. At present by far most of the produced biodiesel is obtained by chemical transesterification reactions, which cannot meet the demands of a totally 'green' fuel production. Therefore, also several biotechnological biodiesel production processes are currently being developed. Biotechnological production can be achieved by purified enzymes in the soluble state, which requires cost-intensive protein preparation. Alternatively, enzymes could be immobilized on an appropriate matrix, enabling a reuse of the enzyme, although the formation of by-products may provide difficulties to maintain the enzyme activity. Processes in presence of organic solvents like t-butanol have been developed, which enhance by-product solubility and therefore prevent loss of enzyme activity. As another approach the application of whole-cell catalysis for the production of fatty acid ethyl esters, which is also referred to as 'microdiesel', by recombinant microorganisms has recently been suggested.
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany.
Stefan
S
Uthoff
Daniel
D
Bröker
Alexander
A
Steinbüchel
D001419:Bacteria / Q000201:enzymology*; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D008049:Lipase / Q000378:metabolism; D012997:Solvents
D056804:Biofuels; D004800:Enzymes, Immobilized; D012997:Solvents; D008049:Lipase
J Agric Food Chem. 2007 Oct 31;55(22):8995-9005
17902621
Bioresour Technol. 2009 Mar;100(6):1983-91
19028094
Biotechnol Lett. 2003 Aug;25(15):1239-41
14514074
Curr Opin Biotechnol. 2003 Aug;14(4):387-94
12943847
Crit Rev Biotechnol. 2008;28(4):253-64
19051104
Bioresour Technol. 2008 Jun;99(9):3900-7
17888652
J Biosci Bioeng. 1999;88(6):627-31
16232675
Microbiology (Reading). 2008 Nov;154(Pt 11):3319-3328
18957585
Appl Biochem Biotechnol. 2006 Feb;128(2):109-16
16484720
J Biotechnol. 2005 Sep 29;119(3):291-9
15950307
J Biosci Bioeng. 2003;95(5):466-9
16233441
J Biosci Bioeng. 2001;91(1):12-5
16232938
Bioprocess Biosyst Eng. 2007 May;30(3):147-55
17252187
Enzyme Microb Technol. 1996 Apr;18(5):340-6
8882001
Science. 2005 Jul 1;309(5731):52-4
15994511
J Bacteriol. 2005 Feb;187(4):1369-76
15687201
Biotechnol Bioeng. 1991 Oct 5;38(7):727-32
18600798
Bioresour Technol. 2007 Apr;98(6):1260-4
16822671
Z Naturforsch C J Biosci. 2008 Mar-Apr;63(3-4):297-302
18533477
Metab Eng. 2008 Nov;10(6):333-9
18812230
Appl Biochem Biotechnol. 2007 Dec;143(3):236-43
18057451
Biotechnol Biofuels. 2009 Jan 14;2(1):1
19144158
Appl Microbiol Biotechnol. 2006 Jun;71(1):13-22
16604360
Appl Biochem Biotechnol. 2004 Spring;113-116:887-98
15054240
Appl Microbiol Biotechnol. 2008 Jun;79(3):331-7
18392617
Appl Microbiol Biotechnol. 2008 Oct;80(5):749-56
18690426
Curr Opin Chem Biol. 2008 Apr;12(2):187-93
18275861
Bioresour Technol. 2005 May;96(7):769-77
15607189
Bioresour Technol. 2007 Feb;98(3):648-53
16630719
Appl Environ Microbiol. 2006 Feb;72(2):1373-9
16461689
J Ind Microbiol Biotechnol. 2008 May;35(5):421
18205018
Appl Microbiol Biotechnol. 2008 Feb;78(1):29-36
18064453
Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):793-803
18478435
Appl Microbiol Biotechnol. 2007 May;75(2):387-95
17225093
Water Sci Technol. 2007;55(5):265-73
17489419
Appl Microbiol Biotechnol. 2008 Dec;81(4):637-45
18795281
Microbiology (Reading). 2006 Sep;152(Pt 9):2529-2536
16946248
Appl Microbiol Biotechnol. 2001 Nov;57(4):515-20
11762598
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2008 May;43(6):589-95
18393066
Biochem Eng J. 2001 Jul;8(1):39-43
11356369
Arch Biochem Biophys. 1981 Sep;210(2):775-85
7030207
J Biotechnol. 2009 Jan 15;139(2):169-75
19007827
Biotechnol Lett. 2006 May;28(9):637-40
16642301
Appl Environ Microbiol. 2005 Feb;71(2):790-6
15691932
Appl Environ Microbiol. 1987 Oct;53(10):2420-5
3322191
J Biosci Bioeng. 2006 Apr;101(4):328-33
16716941
Eur J Biochem. 1986 Jan 2;154(1):119-24
2935393
Trends Biotechnol. 2008 Dec;26(12):668-73
18976825
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Jan;44(1):21-8
19085591
Biotechnol Prog. 2007 Sep-Oct;23(5):1087-90
17691811
Biochem Soc Trans. 2000 Dec;28(6):979-81
11171279
Appl Microbiol Biotechnol. 2004 May;64(4):481-5
14689244
Appl Microbiol Biotechnol. 2003 Dec;63(3):258-66
13680206
Sheng Wu Gong Cheng Xue Bao. 2006 Jan;22(1):114-8
16572850
J Biol Chem. 2003 Mar 7;278(10):8075-82
12502715
Appl Environ Microbiol. 2002 Sep;68(9):4517-22
12200308
Bioresour Technol. 2008 Apr;99(6):1716-21
17531473
Microbiology (Reading). 2002 Nov;148(Pt 11):3328-3329
12427920
Biotechnol Lett. 2004 Jun;26(11):917-21
15269541