Impaired oxidative phosphorylation in overtrained rat myocardium.
The present study was undertaken to characterize and review the changes in energy metabolism in rat myocardium in response to chronic exhaustive exercise. It was shown that a treadmill exercise program applied for six weeks led the rats into a state characterized by decreased performance, loss of body weight and enhanced muscle catabolism, indicating development of overtraining syndrome. Electron microscopy revealed disintegration of the cardiomyocyte structure, cellular swelling and appearance of peroxisomes. Respirometric assessment of mitochondria in saponin-permeabilized cells in situ revealed a decreased rate of oxidative phosphorylation (OXPHOS) due to diminished control over it by ADP and impaired functional coupling of adenylate kinase to OXPHOS. In parallel, reduced tissue content of cytochrome c was observed, which could limit the maximal rate of OXPHOS. The results are discussed with respect to relationships between the volume of work and corresponding energy metabolism. It is concluded that overtraining syndrome is not restricted to skeletal muscle but can affect cardiac muscle as well.
Institute of General and Molecular Pathology, Faculty of Medicine;
Lumme
L
Kadaja
Margus
M
Eimre
Kalju
K
Paju
Mart
M
Roosimaa
Taavi
T
Põdramägi
Priit
P
Kaasik
Ando
A
Pehme
Ehte
E
Orlova
Margareeta
M
Mudist
Nadezhda
N
Peet
Andres
A
Piirsoo
Teet
T
Seene
Frank N
FN
Gellerich
Enn K
EK
Seppet
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