Tailored reference limits for urine corticoid:creatinine ratio in dogs to answer distinct clinical questions.
To establish reference intervals for the urinary corticoid:creatinine ratio (UCCR) determined by chemiluminometric immunoassay, UCCR was measured by this method in 50 healthy dogs. To assess the diagnostic performance of different cut-off levels, the UCCR of 66 dogs with hyperadrenocorticism and 87 dogs with diseases mimicking hyperadrenocorticism were used to construct a receiver operating characteristic (ROC) curve. The upper reference limit derived from morning samples in healthy dogs was 30.81 × 10(-6). The area under the ROC curve was 0.94. The diagnostic cut-off with the highest negative likelihood ratio was 26.5 × 10(-6) (sensitivity 1, specificity 0.54), whereas the cut-off with the highest positive likelihood ratio was 161.2 × 10(-6) (specificity 0.988, sensitivity 0.515). The application of these two different diagnostic cut-offs eliminated the necessity to perform additional tests in 53 per cent of the patient population.
Department for Companion Animals and Horses, Clinic for Small Animals and Infectious Diseases, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria. florian.zeugswetter@vetmeduni.ac.at
F
F
Zeugswetter
N
N
Bydzovsky
D
D
Kampner
I
I
Schwendenwein
D000305:Adrenal Cortex Hormones / Q000652:urine*; D000308:Adrenocortical Hyperfunction / Q000175:diagnosis / Q000652:urine / Q000662:veterinary*; D000818:Animals; D003404:Creatinine / Q000652:urine*; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis* / Q000652:urine; D004285:Dogs / Q000652:urine*; D005260:Female; D008297:Male; D012372:ROC Curve; D012016:Reference Values; D012680:Sensitivity and Specificity
D000305:Adrenal Cortex Hormones; D003404:Creatinine