Transient or persistent norovirus infection does not alter the pathology of Salmonella typhimurium induced intestinal inflammation and fibrosis in mice.
Murine noroviruses (MNV) are currently the most prevalent viruses infecting mouse research colonies. Concurrent infection of research mice with these viruses can dramatically alter the experimental outcome in some research models, but not others. In this report, we investigated the effect of MNV1 and MNV4 on a murine model of intestinal inflammation and fibrosis induced by Salmonella typhimurium infection in C57BL/6 mice. Subsequent co-infection of these mice with MNV1 or MNV4 did not lead to major changes in histopathology, the inflammatory response, or the fibrotic response. Thus, MNV does not substantially alter all gastrointestinal research models, highlighting the importance of investigating potential alterations in the research outcome by MNV on an individual basis. We hypothesize that this is particularly important in cases of research models that use immunocompromised mice, which could be more sensitive to MNV infection-induced changes.
Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Michigan Medical Center, Medical Science Research Building One, 1150 West Medical Center Drive, Ann Arbor, MI 48109-5682, United States. phiggins@med.umich.edu
Peter D R
PD
Higgins
Laura A
LA
Johnson
Kay
K
Sauder
David
D
Moons
Luz
L
Blanco
Stefan
S
Taube
Christiane E
CE
Wobus
D000818:Animals; D058491:Bacterial Load; D017250:Caliciviridae Infections / Q000150:complications* / Q000382:microbiology* / Q000473:pathology; D002432:Cecum / Q000382:microbiology / Q000473:pathology / Q000821:virology; D003094:Collagen / Q000378:metabolism; D003106:Colon / Q000382:microbiology / Q000473:pathology / Q000821:virology; D016207:Cytokines / Q000378:metabolism; D004751:Enteritis / Q000382:microbiology / Q000473:pathology* / Q000821:virology; D005260:Female; D005355:Fibrosis; D005786:Gene Expression Regulation; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D018793:Interleukin-13 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D029322:Norovirus; D012333:RNA, Messenger / Q000378:metabolism; D012480:Salmonella Infections / Q000150:complications* / Q000473:pathology* / Q000821:virology; D012486:Salmonella typhimurium; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology
D016207:Cytokines; D018793:Interleukin-13; D012333:RNA, Messenger; D003094:Collagen
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