Evaluation of the hepatoprotective and antioxidant activities of Rubus parvifolius L.
The hepatoprotective and antioxidant activities of the n-butanol extract of Rubus parvifolius L. (RPL), a widely used medicinal plant, were evaluated. Results demonstrated that RPL extract possessed pronounced hepatoprotective effects against carbon tetrachloride (CCl(4))-induced hepatic injury in mice, which was at least partially attributed to its strong antioxidant capacity. Treatment with RPL extract markedly attenuated the increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels caused by CCl(4) intoxication. It also significantly prevented the decrease in superoxide dismutase (SOD) activity and the increase in malondialdehyde (MDA) content of liver tissue. Meanwhile, histopathological changes of hepatic damage were also remarkably ameliorated. Phytochemical analysis based on high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) revealed the presence of various phenolic compounds, including caffeic acid conjugates, ellagic acid glycosides, and flavonol glycosides, which might be responsible for the hepatoprotective and antioxidant activities of RPL.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Jie
J
Gao
Cui-rong
CR
Sun
Jie-hong
JH
Yang
Jian-mei
JM
Shi
Yue-guang
YG
Du
Yu-yan
YY
Zhang
Jin-hui
JH
Li
Hai-tong
HT
Wan
D020001:1-Butanol; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D027824:Rosaceae / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D053719:Tandem Mass Spectrometry
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D020001:1-Butanol; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
J Ethnopharmacol. 2009 Sep 7;125(2):364-6
19465104
J Asian Nat Prod Res. 2002 Sep;4(3):217-20
12118512
Talanta. 2008 Oct 19;77(1):152-9
18804613
Phytochemistry. 2000 Feb;53(3):405-7
10703066
Biochem Biophys Res Commun. 2005 Apr 15;329(3):991-5
15752753
J Org Chem. 2008 Jul 18;73(14):5233-41
18549269
J Agric Food Chem. 2003 May 21;51(11):3302-8
12744658
J Ethnopharmacol. 2004 Dec;95(2-3):421-4
15507369
Food Chem Toxicol. 2007 Aug;45(8):1349-55
17329003
J Agric Food Chem. 2002 Mar 13;50(6):1379-82
11879006
J Ethnopharmacol. 2008 Jan 4;115(1):61-6
17983713
Phytochemistry. 2008 Nov;69(16):2799-806
18929376
J Agric Food Chem. 2001 Aug;49(8):3651-5
11513642
J Agric Food Chem. 2005 Jul 27;53(15):6003-10
16028988
Phytochem Anal. 2005 Nov-Dec;16(6):451-8
16315490
J Am Soc Mass Spectrom. 2001 Jun;12(6):707-15
11401161
J Mass Spectrom. 2003 Jan;38(1):43-9
12526005
Phytochemistry. 2003 Sep;64(2):617-24
12943785
Food Chem Toxicol. 2008 Sep;46(9):3182-5
18691629
Plant Physiol. 2006 Aug;141(4):1205-18
16896233
J Chromatogr A. 2009 Apr 10;1216(15):3402-8
19249789
J Ethnopharmacol. 2010 Feb 17;127(3):777-80
19874881
Talanta. 2009 May 15;78(3):1090-101
19269477
J Nat Prod. 2001 Mar;64(3):360-3
11277757