Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome.
In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator(®) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.
Cambridge Centre of Neuropsycharitic Research, Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
Xiaoping
X
Yang
Yishai
Y
Levin
Hassan
H
Rahmoune
Dan
D
Ma
Stephanie
S
Schöffmann
Yagnesh
Y
Umrania
Paul C
PC
Guest
Sabine
S
Bahn
D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002853:Chromatography, Liquid; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D011506:Proteins; D020543:Proteome