Immunization with DnaJ (hsp40) could elicit protection against nasopharyngeal colonization and invasive infection caused by different strains of Streptococcus pneumoniae.
Increasing mortality, morbidity and economic costs have been paid to pneumococcal diseases every year. Currently, vaccination is the most promising strategy to reduce the occurrence of pneumococcal infection. In this study, we investigated the protective efficacy of immunization with recombinant DnaJ (hsp40) protein against infections of different serotypes of Streptococcus pneumoniae. We demonstrated that mucosal immunization with DnaJ antigen could induce both systemic and mucosal antibodies for DnaJ and stimulate the release of high levels of IL-10, IFN-γ and IL-17A. Moreover, this mucosal vaccination could reduce nasal or lung colonization of pneumococcus and elicit protection against different serotypes of invasive pneumococcal infections. As well, we found that intraperitoneal immunization with DnaJ could also protect against invasive infections caused by different serotypes of pneumococcus, and passive immunization with antibodies specific for DnaJ confirmed that this protection was antibody-mediated. Our results therefore support the potential of DnaJ as a conserved pneumococcal protein vaccine.
Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, Chongqing Medical University, Department of Laboratory Medicine, Chongqing, PR China.
Yali
Y
Cui
Xuemei
X
Zhang
Yi
Y
Gong
Siqiang
S
Niu
Nanlin
N
Yin
Run
R
Yao
Wenchun
W
Xu
Dairong
D
Li
Hong
H
Wang
Yujuan
Y
He
Ju
J
Cao
Yibing
Y
Yin
D000281:Administration, Intranasal; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D050956:HSP40 Heat-Shock Proteins / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009302:Nasopharyngeal Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D020545:Respiratory Mucosa / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000254:growth & development* / Q000276:immunology* / Q000472:pathogenicity; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
C494641:Dnajb1 protein, mouse; D050956:HSP40 Heat-Shock Proteins; D022242:Pneumococcal Vaccines; D014614:Vaccines, Synthetic