Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme.
A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified magnetic microspheres have a very good core-shell structure and high magnetization.The maximum binding capacity of LZM was about 108.6 mg/g magnetic microspheres. LZM purified from chicken egg white had high purity and well-maintained activity of 8140 U/mg. This magnetic-mediated LZM purification strategy has advantages of high efficiency, low cost and easy operation.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Guoqing
G
Zhang
Qing
Q
Cao
Na
N
Li
Kean
K
Li
Feng
F
Liu
D002021:Buffers; D002846:Chromatography, Affinity; D005290:Ferric Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D009113:Muramidase / Q000737:chemistry*; D014325:Tromethamine / Q000737:chemistry*
D002021:Buffers; D005290:Ferric Compounds; D014325:Tromethamine; C000499:ferric oxide; D009113:Muramidase