Viscoelastic properties of Staphylococcus aureus and Staphylococcus epidermidis mono-microbial biofilms.
The viscoelastic properties of mono-microbial biofilms produced by ocular and reference staphylococcal strains were investigated. The microorganisms were characterized for their haemolytic activity and agr typing and the biofilms, grown on stainless steel surface under static conditions, were analysed by Confocal Laser Scanning Microscopy. Static and dynamic rheometric tests were carried out to determine the steady-flow viscosity and the elastic and viscous moduli. The analysed biofilms showed the typical time-dependent behaviour of viscoelastic materials with considerable elasticity and mechanical stability except for Staphylococcus aureus ATCC 29213 biofilm which showed a very fragile structure. In particular, S. aureus 6ME biofilm was more compact than other staphylococcal biofilms studied with a yield stress ranging between 2 and 3Pa. The data obtained in this work could represent a starting point for developing new therapeutic strategies against biofilm-associated infections, such as improving the drug effect by associating an antimicrobial agent with a biofilm viscoelasticity modifier.
Department of Drug Sciences and Biomedical Sciences, School of Pharmacy, University 'G. d'Annunzio', Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Antonio
A
Di Stefano
Eleonora
E
D'Aurizio
Oriana
O
Trubiani
Rossella
R
Grande
Emanuela
E
Di Campli
Mara
M
Di Giulio
Soraya
S
Di Bartolomeo
Piera
P
Sozio
Antonio
A
Iannitelli
Antonia
A
Nostro
Luigina
L
Cellini
D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D004548:Elasticity; D006461:Hemolysis; D018613:Microscopy, Confocal; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000737:chemistry* / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics; D055682:Viscoelastic Substances / Q000378:metabolism*; D014783:Viscosity
C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; D015534:Trans-Activators; D055682:Viscoelastic Substances
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