Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics.
BACKGROUND
Brassica vegetables have been related to the prevention of cancer and degenerative diseases, owing to their glucosinolate and phenolic content.


OBJECTIVE
Identification of glucosinolates, flavonoids and hydroxycinnamic acids in representative varieties of kale, cabbage and leaf rape.


METHODS
One local variety of each crop was evaluated in this study using a multi-purpose chromatographic method that simultaneously separates glucosinolates and phenolics. Chromatograms were recorded at 330 nm for flavonoid glycosides and acylated derivatives and 227 nm for glucosinolates.


RESULTS
Eight glucosinolates were identified in kale and cabbage, which exhibited the same glucosinolate profile, and 11 glucosinolates were identified in leaf rape. Furthermore, 20 flavonoids and 10 hydroxycinnamic acids were detected in kale and cabbage, while 17 flavonoids and eight hydroxycinnamic acids were found in leaf rape.


CONCLUSIONS
This study has provided a deeper and comprehensive identification of health-promoting compounds in kale, cabbage and leaf rape, thus showing that they are a good source of glucosinolates and phenolic antioxidants.
Misión Biológica de Galicia (CSIC), PO Box 28, E-36080 Pontevedra, Spain. pvelasco@mbg.cesga.es
Pablo
P
Velasco
Marta
M
Francisco
Diego A
DA
Moreno
Federico
F
Ferreres
Cristina
C
García-Viguera
María Elena
ME
Cartea
D001937:Brassica / Q000737:chemistry*; D029688:Brassica napus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005961:Glucosinolates / Q000737:chemistry*; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D014675:Vegetables / Q000737:chemistry
D003373:Coumaric Acids; D005419:Flavonoids; D005961:Glucosinolates