Rapid detection of Vibrio metschnikovii in aquatic products by real-time PCR.
PCR and SYBR Green I real-time PCR techniques were applied to the rapid detection of Vibrio metschnikovii by designing primers based on infC (initiation factor 3) gene sequence. The specificity, sensitivity, and practical applications of the methods have been also analyzed. The methods showed high detecting specificity with no cross amplifications of other closely related and nonrelated species; they provide a simple and rapid tool for V. metschnikovii detection with high sensitivity and specificity.
Liaoning Entry-Exit Inspection & Quarantine Bureau, Dalian 116001, China. cjj0909@163.com
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J
Cao
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J
Xu
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Q
Zheng
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P
Yan
D001431:Bacteriological Techniques / Q000379:methods*; D052160:Benzothiazoles; D017931:DNA Primers / Q000235:genetics; D003959:Diamines; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D039665:Prokaryotic Initiation Factor-3 / Q000235:genetics; D011804:Quinolines; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D014733:Vibrio / Q000302:isolation & purification*; D014871:Water Microbiology
D052160:Benzothiazoles; D017931:DNA Primers; D003959:Diamines; D009930:Organic Chemicals; D039665:Prokaryotic Initiation Factor-3; D011804:Quinolines; C098022:SYBR Green I
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