Up-regulation of AMP-activated protein kinase in cancer cell lines is mediated through c-Src activation.
We report that the activation level of AMP-dependent protein kinase AMPK is elevated in cancer cell lines as a hallmark of their transformed state. In OVCAR3 and A431 cells, c-Src signals through protein kinase Cα, phospholipase Cγ, and LKB1 to AMPK. AMPK controls internal ribosome entry site (IRES) dependent translation in these cells. We suggest that AMPK activation via PKC might be a general mechanism to regulate IRES-dependent translation in cancer cells.
Unit of Cellular Signaling, Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Sarit
S
Mizrachy-Schwartz
Noam
N
Cohen
Shoshana
S
Klein
Nataly
N
Kravchenko-Balasha
Alexander
A
Levitzki
D055372:AMP-Activated Protein Kinases / Q000235:genetics*; D000081247:CSK Tyrosine-Protein Kinase; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004789:Enzyme Activation / Q000502:physiology; D006801:Humans; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation / Q000235:genetics*; D019061:src-Family Kinases
D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases; C000631346:CSK protein, human; D055372:AMP-Activated Protein Kinases
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