Pleural fluid from tuberculous pleurisy inhibits the functions of T cells and the differentiation of Th1 cells via immunosuppressive factors.
Immunosuppressive mediators in tuberculosis pleurisy (pleural fluid (PF)) are associated with the course of disease, but they remain poorly defined. To study the local immune status of patients with tuberculosis pleurisy, we examined the effect of PF on the functions of T cells and the differentiation of Th1 cells. PF could inhibit the ability of T cells to produce cytokines. However, tumor-necrosis factor (TNF)-α derived from non-T cells was not impaired. Further analysis indicated that cell activation and cell cycle progression were also suppressed. Moreover, PF could inhibit Th1 cell differentiation. Importantly, we found that inhibitors of indoleamine 2,3-dioxygenase (IDO) and adenosine and neutralizing antibodies against IL-10 and transforming growth factor (TGF)-β could reverse cytokine production, suggesting that IDO, adenosine, IL-10 and Transforming growth factor-β1 in PF might take part in impairing T-cell functions. Taken together, our data demonstrate for the first time that several immunopathological factors participate in the downregulation of T-cell functions in local PF.
Department of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-Sen University, Guangzhou, China. changyou_wu@yahoo.com
Qin
Q
Li
Li
L
Li
Yun
Y
Liu
Xiaoying
X
Fu
Dan
D
Qiao
Hui
H
Wang
Suihua
S
Lao
Fengyu
F
Huang
Changyou
C
Wu
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001826:Body Fluids / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002453:Cell Cycle; D002454:Cell Differentiation / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction; D018417:Th1 Cells / Q000276:immunology* / Q000473:pathology*; D014396:Tuberculosis, Pleural / Q000276:immunology*; D055815:Young Adult
D016207:Cytokines; D007166:Immunosuppressive Agents
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