Screening of reducing agents for anaerobic growth of Candida albicans SC5314.
This study aimed to evaluate the influence of different redox potentials (Eh) on cell growth, whole-cell protein profile and cell surface hydrophobicity (CSH) of Candida albicans SC5314. The yeast was grown in YNB broth enriched with reducing (158mM sodium sulfite, 4mM sodium sulfite, 2.5mM sodium metabisulfite, 1.3mM 2-mercaptoethanol, 5.5mM thioglycolic acid, and 3.2mM l-cysteine hydrochloride) and oxidizing agents (15mM ammonium persulfate and 80mM potassium ferricyanide) and incubated in normoxic and anoxic atmospheres at 37°C, for 48h. Pre- and post-incubation Eh values were determined and cytoplasm proteins were extracted. Proteins were parted by SDS-PAGE and their profiles were compared. 3.2mM l-cysteine and 1.3mM 2-mercaptoethanol promoted and maintained negative Eh values during incubation. No differences were detected among SDS-PAGE profiles. CSH differences only were observed with 4mM sodium sulfite and 3.2mM l-cysteine. Results showed that 3.2mM l-cysteine is a reducing agent that allows maintenance of negative Eh in both anoxic and normoxic conditions and it seems not to interfere in the global expression of plasmatic proteins.
Xenobiotics Research Unit, Biological and Health Sciences Center, The Pontifical Catholic University of Paraná, Brazil.
A U M
AU
Rymovicz
R D
RD
Souza
L C
LC
Gursky
R T
RT
Rosa
P C
PC
Trevilatto
F C
FC
Groppo
E A R
EA
Rosa
D000693:Anaerobiosis; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry; D005656:Fungal Proteins / Q000032:analysis; D008828:Microbiological Techniques / Q000379:methods*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D020543:Proteome / Q000032:analysis; D019163:Reducing Agents / Q000378:metabolism*
D003470:Culture Media; D005656:Fungal Proteins; D016877:Oxidants; D020543:Proteome; D019163:Reducing Agents