Effects of optimized-SopungSunkiwon on memory impairment and enhancement.
Optimized-SopungSunkiwon (OSS) is a multi-herbal formula that contains six medicinal herbs from SopungSunkiwon, a traditional medicine used for neurodegenerative disorders. In this study, we investigated the anti-amnesic effects of OSS in a dementia model. Acetylcholinesterase (AChE) inhibition assay was performed to investigate the cholinergic antagonistic effect of OSS. In addition, a step-through passive-avoidance test was performed with scopolamine-induced memory impairment in mice, and immunohistochemistry was analyzed to investigate synaptic formation with synaptic proteins. OSS inhibited AChE activity, resulting in significant improvement of memory functions. In the passive-avoidance test, the latency time of OSS-treated mice was significantly longer than that of either the control or scopolamine-treated group. In the immunohistochemical analysis, synaptic proteins such as synaptophysin and PSD-95 were significantly increased in OSS-treated mice. These results demonstrate that OSS may affect impairment and enhancement of memory and increase synaptophysin and PSD-95 facilitating acetylcholine release and synaptic growth.
Department of Oriental Pharmaceutical Science, College of Pharmacy and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, #1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Jin Gyu
JG
Choi
Woong Mo
WM
Yang
Tong Ho
TH
Kang
Myung Sook
MS
Oh
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology; D001362:Avoidance Learning / Q000187:drug effects; D001921:Brain / Q000187:drug effects*; D002338:Carotenoids / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D007150:Immunohistochemistry; D039823:Iridoids / Q000494:pharmacology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D055670:Medicine, Korean Traditional; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000188:drug therapy; D051379:Mice; D008813:Mice, Inbred ICR; D018696:Neuroprotective Agents / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal; D012676:Senna Extract; D000081226:Sennosides
D000880:Anthraquinones; D039823:Iridoids; D018696:Neuroprotective Agents; D010936:Plant Extracts; D000081226:Sennosides; C007835:geniposide; D002338:Carotenoids; D012676:Senna Extract; C029036:crocin; D000110:Acetylcholinesterase