Inhibitory effects of prior low-dose X-irradiation on cold-induced brain injury in mouse.
We examined the inhibitory effects of low-dose X-irradiation on mouse brain tissue with cold-induced injury by comparing tissue samples from three groups of mice: control, sham-irradiated cold-exposed, and X-ray-irradiated (0.5 Gy) cold-exposed mice. The water content in brain increased significantly in the sham-irradiated group following the cold-induced injury relative to the control group. However, water content in brain tissue from the X-ray-irradiated group was significantly lower than that from the sham-irradiated group. Levels of antioxidants, such as superoxide dismutase and glutathione, in brain tissue from the X-ray-irradiated group were higher than those from the sham-irradiated group. Moreover, the cold injury-induced cell death, particularly apoptosis, while low-dose irradiation inhibited cell death, especially among glial cells, but not numeral cells. These findings suggest that prior low-dose X-irradiation activated antioxidant function and inhibited cold-induced brain injury.
Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho 2-chome, Kita-ku, Okayama-shi, Okayama, 700-8558, Japan.
Masaaki
M
Yoshimoto
Takahiro
T
Kataoka
Teruaki
T
Toyota
Takehito
T
Taguchi
Kiyonori
K
Yamaoka
D000818:Animals; D000975:Antioxidants; D017209:Apoptosis; D001921:Brain / Q000528:radiation effects*; D001929:Brain Edema / Q000209:etiology / Q000517:prevention & control*; D001930:Brain Injuries / Q000150:complications / Q000378:metabolism / Q000532:radiotherapy*; D003080:Cold Temperature; D005978:Glutathione / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009457:Neuroglia / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects*; D011829:Radiation Dosage; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014965:X-Rays
D000975:Antioxidants; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D005978:Glutathione
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