Integrative expression system for delivery of antibody fragments by lactobacilli.
A series of expression cassettes which mediate secretion or surface display of antibody fragments was stably integrated in the chromosome of Lactobacillus paracasei. L. paracasei producing surface-anchored variable domain of llama heavy chain (VHH) (ARP1) directed against rotavirus showed efficient binding to rotavirus and protection in the mouse model of rotavirus infection.
Instituto de Productos Lácteos de Asturias (IPLA, CSIC), 3300 Villaviciosa, Asturias, Spain.
M Cruz
MC
Martín
Neha
N
Pant
Victor
V
Ladero
Gökçe
G
Günaydin
Kasper Krogh
KK
Andersen
Beatriz
B
Alvarez
Noelia
N
Martínez
Miguel A
MA
Alvarez
Lennart
L
Hammarström
Harold
H
Marcotte
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007128:Immunoglobulin Fragments / Q000235:genetics* / Q000502:physiology; D007778:Lactobacillus / Q000235:genetics*; D052197:Limosilactobacillus fermentum / Q000235:genetics / Q000276:immunology; D051379:Mice; D012400:Rotavirus Infections / Q000276:immunology / Q000628:therapy*
C494788:APF protein, Lactobacillus crispatus; D001426:Bacterial Proteins; D007128:Immunoglobulin Fragments
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