Evaluation of CHROMagar™ StrepB agar, an aerobic chromogenic medium for prepartum vaginal/rectal Group B Streptococcus screening.
An aerobic chromogenic medium, CHROMagar™ StrepB agar, designed for isolation of group B Streptococci, was evaluated on 285 prepartum vaginal/rectal swabs from pregnant women. After overnight enrichment in Todd-Hewitt broth containing 15μg/ml nalidixic acid and 10μg/ml colistin, sensitivities were respectively 79% on day 1 and 92% on day 2, and significantly higher than those achieved by blood agar (40% and 58%) and colimycin-nalidixic-acid agar (82% on day 2).
Microbiology Laboratory, Centre Hospitalier Régional Orléans, BP 86709, F-45067, France. didier.poisson@chr-orleans.fr
Didier-Marc
DM
Poisson
Marie-Liesse
ML
Evrard
Claire
C
Freneaux
Marie-isabelle
MI
Vivès
Louis
L
Mesnard
D000362:Agar; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D011247:Pregnancy; D012007:Rectum / Q000382:microbiology*; D013290:Streptococcal Infections / Q000175:diagnosis*; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology*
D003470:Culture Media; D000362:Agar