An abiotic stress response gene TaSnRK2.7-B in wheat accessions: genetic diversity analysis and gene mapping based on SNPs.
TaSnRK2.7, a SnRK2 (sucrose non-fermenting1-related protein kinase 2) member of wheat, confers enhanced multi-stress tolerance in carbohydrate metabolism. Here, the nucleotide diversity of TaSnRK2.7 was investigated in 60 wheat accessions with different stress-tolerant features. One copy, named TaSnRK2.7-B, was detected in 32 accessions, which mainly exhibited stress tolerance. The TaSnRK2.7-B evolutionary dynamics were examined in these cultivars, which have been subjected to population bottlenecks and intensive selection in breeding. Nucleotide diversity (π) in the entire TaSnRK2.7-B region was 0.00148. Sliding-window analysis demonstrated that 3932-4535bp was a variation-enriched region, covering the eighth intron, the ninth exon and the 3'-flanking region. As predicted, neutrality tests revealed that the TaSnRK2.7-B protein was relatively conservative, and population bottlenecks or purifying selection may have acted on TaSnRK2.7-B. Furthermore, linkage disequilibrium between SNP loci extends across the entire TaSnRK2.7-B region. A SNP marker of TaSnRK2.7-B was developed and mapped on chromosome 2AL flanked by WMC179.4 and WMC401, which were co-located in the same or adjacent chromosome intervals with QTLs for phosphorus utilization efficiency and accumulation efficiency of stem water-soluble carbohydrates.
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Hongying
H
Zhang
Xinguo
X
Mao
Xianshan
X
Wu
Chengshe
C
Wang
Ruilian
R
Jing
D001947:Breeding; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D056426:Genetic Loci; D018745:Genome, Plant; D007438:Introns; D015810:Linkage Disequilibrium; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D018744:DNA, Plant; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases