Differential expression of signaling pathways in odontogenic differentiation of ectomesenchymal cells isolated from the first branchial arch.
The aim of this study was to screen for differential expression of signaling pathways in odontogenic differentiation of ectomesenchymal cells isolated from the first branchial arch of embryonic day 10 (E10) mice by real time RT-PCR microarray. Observations of cellular morphology, immunocytochemistry, and RT-PCR were used to identify the cell source. A real time RT-PCR microarray was then used to detect the differential expression of signaling pathways in cells dissected from animals at two different developmental stages. These assays identified 25 up-regulated genes and 16 down-regulated genes involved in odontogenic differentiation of the ectomesenchymal cells of the first branchial arch. They represented the main members of Wnt, Hedgehog, TGF-β, NF-κB, and LDL signaling pathways. This study determined that these signaling pathways are important for odontogenic differentiation of ectomesenchymal cells of the first branchial arch.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People's Republic of China.
Yongchun
Y
Yu
Mingheng
M
Li
Jie
J
Sun
Miaomiao
M
Yang
Jie
J
Long
Weidong
W
Tian
Wei
W
Tang
Tangxin
T
Li
Lei
L
Liu
D000818:Animals; D001483:Base Sequence; D001934:Branchial Region / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D017931:DNA Primers; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
D017931:DNA Primers
Int J Dev Biol. 1995 Feb;39(1):35-50
7626420
Histol Histopathol. 2002 Apr;17(2):497-505
11962755
Mol Cell Biochem. 2008 Feb;309(1-2):143-50
18008138
Development. 2000 Apr;127(8):1671-9
10725243
Dev Biol. 2008 Jan 1;313(1):210-24
18022614
J Biol Chem. 2006 Jul 7;281(27):18363-9
16651263
Circ Res. 2002 May 31;90(10):1055-63
12039794
Genes Dev. 1998 Sep 1;12(17):2735-47
9732271
Development. 2001 Nov;128(22):4605-13
11714685
J Mol Histol. 2008 Feb;39(1):87-94
17786571
Int J Dev Biol. 2006;50(6):511-21
16741866
Eur J Histochem. 2008 Oct-Dec;52(4):243-50
19109099
Trends Genet. 1999 Feb;15(2):59-65
10098408
J Embryol Exp Morphol. 1970 Aug;24(1):173-86
5487155
Arch Oral Biol. 2010 Jun;55(6):426-34
20392435
Nature. 2001 Feb 15;409(6822):922-7
11237012
J Dent Res. 2000 Apr;79(4):920-5
10831093
Science. 1998 Nov 6;282(5391):1136-8
9804553
Development. 2006 Mar;133(6):1045-57
16481354
Dev Cell. 2002 May;2(5):643-53
12015971
Nat Cell Biol. 2007 Apr;9(4):361-3
17401385
OMICS. 2009 Aug;13(4):337-43
19422292
Dev Biol. 2008 Aug 1;320(1):19-29
18572160
Development. 1998 Aug;125(15):2803-11
9655803
Eur J Oral Sci. 2002 Apr;110(2):114-20
12013553
J Exp Zool B Mol Dev Evol. 2009 Jun 15;312B(4):309-19
19156667
Int Rev Immunol. 2008;27(5):293-319
18853341
Endocr Rev. 2002 Dec;23(6):787-823
12466190
J Dent Res. 2005 Jul;84(7):603-6
15972586
J Cell Biochem. 2008 Jun 1;104(3):733-44
18253935
Arch Oral Biol. 2005 Feb;50(2):159-63
15721144
Free Radic Res. 2004 Jun;38(6):541-51
15346645
Annu Rev Cell Dev Biol. 2004;20:781-810
15473860
Eur J Cell Biol. 2007 Dec;86(11-12):781-99
17499880
Oncogene. 2007 Feb 1;26(5):711-24
16862183
Methods. 2001 Dec;25(4):402-8
11846609
Clin Oral Implants Res. 2007 Jun;18 Suppl 3:2-14
17594365
J Cell Biol. 2000 Oct 2;151(1):117-30
11018058
Arch Oral Biol. 2007 Sep;52(9):814-21
17391645
Arch Oral Biol. 1987;32(2):123-7
3478009
OMICS. 2009 Aug;13(4):331-6
19422302
Dev Biol. 2005 Feb 1;278(1):130-43
15649466
Development. 2008 Jan;135(2):353-65
18077587
Development. 2000 Nov;127(22):4775-85
11044393
Arch Oral Biol. 2005 Feb;50(2):137-40
15721140
Development. 1998 Jan;125(2):161-9
9486790