Flow-injection chemiluminescent determination of estrogen benzoate using the tris(1,10-phenanthroline) ruthenium(II)-permanganate system.
Chemiluminescence (CL) detection for the determination of estrogen benzoate, using the reaction of tris(1,10-phenanthroline)ruthenium(II)-Na(2)SO(3)-permanganate, is described. This method is based on the CL reaction of estrogen benzoate (EB) with acidic potassium permanganate and tris(1,10-phenanthroline)ruthenium(II). The CL intensity is greatly enhanced when Na(2)SO(3) is added. After optimization of the different experimental parameters, a calibration graph for estrogen benzoate is linear in the range 0.05-10 µg/mL. The 3 s limit of detection is 0.024 µg/mL and the relative standard deviation was 1.3% for 1.0 µg/mL estrogen benzoate (n = 11). This proposed method was successfully applied to commercial injection samples and emulsion cosmetics. The mechanism of CL reaction was also studied.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan, People's Republic of China.
Yan
Y
Ma
Wei
W
Cao
Shuang
S
Qiao
Wenwen
W
Liu
Jinghe
J
Yang
D004967:Estrogens / Q000032:analysis*; D017022:Flow Injection Analysis / Q000379:methods*; D049449:Luminescence; D017895:Manganese Compounds / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*
D004967:Estrogens; D017895:Manganese Compounds; D010087:Oxides; C048856:permanganic acid