[Effects of TGF-β1 on gene expression of connective tissue growth factor in lung fibroblasts].
OBJECTIVE
To study the effects of transforming growth factor-β1 (TGF-β1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro.


METHODS
Wistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-β1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs.


RESULTS
Compared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment (P<0.05).


CONCLUSIONS
TGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
Department of Pediatrics, Shengjing Hospital, China Medical University, Shenyang 110004, China. fujh@sj-hospital.org
Jian-Hua
JH
Fu
Hai-Ping
HP
Yang
Li
L
Pan
Xin-Dong
XD
Xue
Hong
H
Gao
D000818:Animals; D055513:Connective Tissue Growth Factor / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D011658:Pulmonary Fibrosis / Q000209:etiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor