Antifungal properties of crude extracts of five Egyptian medicinal plants against dermatophytes and emerging fungi.
Antifungal properties of the crude extracts of five medicinal plants (Artemisia judaica, Ballota undulate, Cleome amblyocarpa, Peganum harmala, and Teucrium polium) were tested against dermatophytes and emerging fungi. Ethanol extract of Ballota undulate was the most effective against all tested fungi. Paecilomyces lilacinus, P. variotii, and Candida albicans were the most sensitive organisms. The minimum inhibitory concentration (MIC) of Ballota undulate ethanol extract against C. albicans, P. lilacinus, and P. variotii was 25 mg/ml. GC-MS analysis revealed that Ballota undulate ethanol extract contains 35 aliphatic and aromatic hydrocarbons, sesquiterpene hydrocarbon along with some other essential oils, which could be involved in antifungal activity. Light microscopy and scanning electron microscopy (SEM) have proved that Ballota undulate ethanol extract exhibits fungicidal effect on P. lilacinus through alterations in hyphal structures including budding of hyphal tip, anomalous structure, such as swelling, decrease in cytoplasmic content, with clear separation of cytoplasm from cell wall in hyphae. SEM clearly showed distorted mycelium, squashed and flattened conidiophores bearing damaged metullae. Eventually, the mycelia became papillated, flattened, and empty. Puncturing and squashing of hyphae as well as complete cell wall disruption were clear signs of complete death of hyphae.
Botany Department, Faculty of Science, Assiut University, Assiut, Egypt. drmhashem69@yahoo.com
Mohamed
M
Hashem
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D045424:Complex Mixtures / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004534:Egypt; D008401:Gas Chromatography-Mass Spectrometry; D025301:Hyphae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008853:Microscopy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
D000935:Antifungal Agents; D045424:Complex Mixtures; D010936:Plant Extracts
J Ethnopharmacol. 2001 Jan;74(1):41-4
11137346
Phytochemistry. 1994 Jul;36(4):971-4
7765213
J Ethnopharmacol. 2002 Nov;83(1-2):129-34
12413718
Clin Infect Dis. 1995 Nov;21(5):1077-89; quiz 1090-1
8589125
Microbiol Res. 2008;163(3):337-44
16870411
J Ethnopharmacol. 2008 Jan 4;115(1):140-6
17996411
J Ethnopharmacol. 2005 Aug 22;100(1-2):80-4
15964727
AIDS Patient Care STDS. 2001 Aug;15(8):407-10
11522214
Can J Microbiol. 2001 Jan;47(1):9-17
15049444
J Ethnopharmacol. 2001 Apr;75(1):45-50
11282442
J Ethnopharmacol. 1997 Mar;56(1):81-7
9147258
J Ethnopharmacol. 2003 Jun;86(2-3):235-41
12738093
J Ethnopharmacol. 2006 Jan 3;103(1):139-42
16271287
Infect Dis Clin North Am. 2002 Dec;16(4):915-33, vi-vii
12512187
J Ethnopharmacol. 2000 Nov;73(1-2):191-8
11025156
J Am Acad Dermatol. 1998 Sep;39(3):401-9
9738773
Mycoses. 1999;42(11-12):665-72
10680445
Microbiol Res. 2004;159(3):295-304
15462529
N Engl J Med. 1998 Jun 11;338(24):1741-51
9624195
J Ethnopharmacol. 2005 Jun 3;99(2):301-8
15894142
Annu Rev Phytopathol. 2002;40:221-49
12147760