Using the liquid nature of the stationary phase. VI. Theoretical study of multi-dual mode countercurrent chromatography.
Countercurrent chromatography (CCC) is a separation technique using a biphasic liquid system and centrifugal forces to maintain a support-free liquid stationary phase. Either one of the two phases can be the liquid stationary phase. It is even possible to switch the phase role during the separation. The dual-mode method is revisited recalling its theoretical background. The multi-dual mode (MDM) CCC method was introduced to enhance the resolution power of a CCC column. The theoretical study of the MDM method is validated by modeling the separation of two solutes. The basic hypothesis is that the forward step (partial classical elution) is followed by a backward step that returns the less retained solute to the column head. The equations show that the most important parameter to maximize resolution is not the number of MDM steps but the total volume of liquid phases used to elute the solutes. The model is validated calculating correctly the peak position of previously published MDM experiments.
Laboratoire des Sciences Analytiques, Université de Lyon 1, CNRS, Bat. CPE, 69622 Villeurbanne, France.
Nazim
N
Mekaoui
Alain
A
Berthod
D000327:Adsorption; D000596:Amino Acids / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods; D012117:Resins, Synthetic / Q000737:chemistry
D000596:Amino Acids; D012117:Resins, Synthetic