[Genetic diversity and phylogeny of soybean rhizobia isolated from the regions of Loess Plateau in China].
OBJECTIVE
We investigated the genetic diversity and phylogeny of soybean rhizobia isolated from the regions of Loess Plateau in China.


METHODS
We analyzed 130 soybean rhizobia isolated from 15 regions in 4 provinces of Loess Plateau through BOX-PCR, 16S rDNA PCR- RFLP, 16S-23S IGS PCR-RFLP and 16S rRNA gene sequencing.


RESULTS
BOX-PCR, 16S rDNA PCR- RFLP and 16S-23S IGS PCR-RFLP were in good agreement with the results which showed that all strains tested ascribed to two groups: the genus of Sinorhizobium and Bradyrhizobium phylogenetically. The analysis of 16S rRNA gene of 5 representative strains indicated that they were related to type strains S. fredii, B. japonicum and B. liaoningense, homology coefficient with type strains was 100% respectively.


CONCLUSIONS
Soybean rhizobia isolated from the regions of Loess Plateau in China showed rich genetic diversity. S. fredii was the dominant species. Bradyrhizobium accounted for 10% of the strains tested only, of which, two strains were B. liaoningense.
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China. zhanghongxia2004@126.com
Hongxia
H
Zhang
Ruihua
R
Feng
Jun
J
Li
Dawei
D
Guan
Fengming
F
Cao
D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020579:Sinorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S