miR-153 sensitized the K562 cells to As2O3-induced apoptosis.
Relapse remains the biggest hurdle of leukemia therapy, while elucidating the molecular mechanism holds promise for the solution. Recently, microRNAs are emerging as an important regulator of cell function. In this study, we for the first time found that miR-153 was downregulated in As2O3-induced drug-resistant K562 cells. In the CD34+ K562 subpopulation, which is characteristic of leukemia stem cell and resembles the drug-resistant subgroup, miR-153 expression level was also much lower than that in the bulk. Forced expression of miR-153 only in K562 cells has no significant effects on cell growth and apoptosis. However, when cells were additionally treated with As2O3, significant greater apoptosis was observed in the miR-153 overexpressed group. Our data here suggest that strategies increasing the endogenous miR-153 might hold promise for an alternative adjuvant therapy of leukemia.
Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xinshi Road No1, 710038 Baqiao District, Xi'an, China. liuli1@medmail.com.cn
Li
L
Liu
Renan
R
Chen
Siyong
S
Huang
Yanlan
Y
Wu
Guohui
G
Li
Bei
B
Zhang
Qiang
Q
Liu
Dandan
D
Yin
Yingmin
Y
Liang
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D010087:Oxides / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction
D000970:Antineoplastic Agents; D001152:Arsenicals; C556500:MIRN153 microRNA, human; D035683:MicroRNAs; D010087:Oxides; D000077237:Arsenic Trioxide
Nature. 2007 Oct 11;449(7163):682-8
17898713
Med Oncol. 2011 Sep;28(3):835-9
20407854
Blood. 2004 Dec 15;104(13):4219-25
15339852
Leukemia. 2010 Sep;24(9):1545-54
20574455
Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15805-10
17890317
Curr Opin Genet Dev. 2006 Feb;16(1):4-9
16361094
Br J Haematol. 2001 Apr;113(1):11-23
11328274
Blood. 2004 Jun 1;103(11):4010-22
14982876
Best Pract Res Clin Haematol. 2009 Sep;22(3):367-79
19959087
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3378-83
19208803
Cell. 2004 Jan 23;116(2):281-97
14744438
N Engl J Med. 2003 Oct 9;349(15):1451-64
14534339
Nature. 1973 Jun 1;243(5405):290-3
4126434
Biochem Biophys Res Commun. 2010 Dec 10;403(2):203-8
21056550
Cancer Sci. 2010 Jul;101(7):1577-81
20491777
Int J Cancer. 2010 Feb 15;126(4):1029-35
19676043
Leukemia. 2005 Jun;19(6):1034-41
15815728
Crit Rev Clin Lab Sci. 2006;43(1):1-67
16531274
Med Oncol. 2011 Mar;28(1):211-8
20143188