Influence of polysaccharide composition on the biocompatibility of pullulan/dextran-based hydrogels.
The implantation of a biomaterial for tissue engineering requires the presence of a suitable scaffold on which the tissue repair and regeneration will take place. Polymers have been frequently used for that purpose because they show similar properties to that of the natural extracellular matrix. Scaffold properties and biocompatibility are modulated by the composition of the polymers used. In this work four polysaccharide-based hydrogels (PSH) made of dextran and pullulan were synthesized. Their in vitro properties were determined and then tested in vivo in a rat model. As pullulan concentration increased in dextran hydrogels, the glass transition temperature and the maximum modulus decreased. In vitro degradation studies for 30 days demonstrated no significant degradation of PSH except for 100% pullulan hydrogel. In vivo tissue response evaluated 30 days after PSH subcutaneous implantation in rats indicated that all PSH were surrounded by a fibrous capsule. Adding pullulan to dextran induced an increased inflammatory reaction compared to PSH-D(100% dextran) or PSH-D(75)P(25)(75% dextran). This in vitro and in vivo data can be used in the design of hydrogels appropriate for tissue engineering applications.
INSERM, U 698, CHU Xavier Bichat, Bât Inserm, 46 rue Henri Huchard, 75018 Paris, France.
Aicha
A
Abed
Nabila
N
Assoul
Maguette
M
Ba
Sidi Mohamed
SM
Derkaoui
Patrick
P
Portes
Liliane
L
Louedec
Patrice
P
Flaud
Isabelle
I
Bataille
Didier
D
Letourneur
Anne
A
Meddahi-Pellé
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology*; D055119:Elastic Modulus / Q000187:drug effects; D005936:Glucans / Q000494:pharmacology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D008422:Materials Testing / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D011134:Polysaccharides / Q000737:chemistry*; D019919:Prosthesis Implantation; D051381:Rats; D017208:Rats, Wistar; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000473:pathology
D001672:Biocompatible Materials; D003911:Dextrans; D005936:Glucans; D011134:Polysaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C009109:pullulan