Human umbilical cord-derived Schwann-like cell transplantation combined with neurotrophin-3 administration in dyskinesia of rats with spinal cord injury.
Mesenchymal stem cells are capable of differentiating into Schwann-like cells. In this study, we induced human umbilical-cord mesenchymal stem cells (HUMSCs) in vitro into neurospheres constituted by neural stem-like cells, and further into cells bearing strong morphological, phenotypic and functional resemblances with Schwann-like cells. These HUMSC-derived Schwann-like cells, after grafting into the injured area of the rats' spinal cord injury (SCI), showed a partial therapeutic effect in terms of improving the motor function. Neurotrophin-3 (NT-3) was reported to improve the local microenvironment of the grafted cells, and we, therefore, further tested the effect of Schwann-like cell grafting combined with NT-3 administration at the site of cell transplantation. The results showed that NT-3 administration significantly promoted the survival of the grafted cells in the host-injured area. Significant improvement in rats treated by Schwann-like cell grafting combined with NT-3 administration was demonstrated in the behavioral test as compared with that in animal models received the cell grafting only. These results suggest that transplantation of the Schwann-like cells combined with NT-3 administration may represent a new strategy of stem cell therapy for spinal cord injury.
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510282 Guangzhou, China.
Guo
G
Yan-Wu
Ke
K
Yi-Quan
Li
L
Ming
Cai
C
Ying-Qian
Jiang
J
Xiao-Dan
Zhang
Z
Shi-Zhong
Zhang
Z
Wang-Ming
Duan
D
Chuan-Zhi
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D017690:Cell Transplantation; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D020820:Dyskinesias / Q000628:therapy*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D020933:Neurotrophin 3 / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology*; D013119:Spinal Cord Injuries / Q000628:therapy*; D014470:Umbilical Cord / Q000166:cytology*
D020933:Neurotrophin 3
Neurosci Lett. 1985 Oct 24;61(1-2):79-84
3935983
Exp Neurol. 2007 Oct;207(2):267-74
17761164
J Neurosci Res. 2007 Nov 1;85(14):3064-70
17638301
Biomaterials. 2010 May;31(14):3848-57
20149437
Brain Res. 2009 Jun 18;1276:39-49
19389387
Acta Neurochir (Wien). 2005 Sep;147(9):985-92; discussion 992
16010451
Stem Cells Dev. 2010 Jan;19(1):117-30
19619003
Stem Cells. 2006 Jan;24(1):115-24
16099997
Gene Expr. 2008;14(4):241-50
19110723
In Vitro Cell Dev Biol Anim. 2008 Jan-Feb;44(1-2):31-40
17985192
Prog Brain Res. 2004;146:243-63
14699968
Exp Neurol. 2010 Jun;223(2):537-47
20153320
J Comp Neurol. 2008 Dec 20;511(6):724-35
18925574
J Am Soc Nephrol. 2006 Aug;17(8):2202-12
16790513
Nat Protoc. 2008;3(12):1888-94
19008875
J Neurol Sci. 2007 Apr 15;255(1-2):81-6
17360004
Cell Mol Neurobiol. 2008 Jun;28(4):529-43
17713852
Biochem Biophys Res Commun. 2007 Jun 29;358(2):637-43
17499609
Differentiation. 2010 Jan;79(1):15-20
19800163
Neuroreport. 2009 Mar 4;20(4):354-9
19223795
J Neurotrauma. 1996 Jul;13(7):343-59
8863191
Cell Transplant. 2005;14(7):489-96
16285257
Neuroscience. 1994 Nov;63(1):57-72
7898661
Cytotherapy. 2009;11(2):163-76
19152152
Stem Cells. 2008 Mar;26(3):591-9
18065397