Morphological behavior and attachment of p19 neural cells to root-end filling materials.
Some techniques and instruments like stereomicroscopy and confocal microscopy used for observing neural cells are too complicated and dependent on preparation and cell fixation methods. This may question the results of these methods. Though, we have used scanning electron microscopy on replicated specimens to observe p19 neural cells and their cellular extensions. This manuscript has shown the feasibility of using replica (indirect) method instead of direct methods for observing morphological characteristics of this high sensitive cell line. As neural cells are very sensitive to fixation solutions and processes, we have used replica mode and observed neural cells with a novel indirect method. We have used replica mode in this study to indirectly and noninvasively evaluate the state of p19 neural cells and their cellular extensions.
Department of Dental Material, Dental Branch, Islamic Azad University, Tehran, Iran. saghiri@gmail.com
Mohammad Ali
MA
Saghiri
Kamal
K
Asgar
Morteza
M
Daliri
Mehrdad
M
Lotfi
Abbas
A
Delvarani
Payman
P
Mehrvarzfar
Kasra
K
Karamifar
D002460:Cell Line; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation / Q000379:methods*
D012387:Root Canal Filling Materials