The microRNA expression profiles of mouse mesenchymal stem cell during chondrogenic differentiation.
MicroRNAs are potential key regulators in mesenchymal stem cells chondrogenic differentiation. However, there were few reports about the accurate effects of miRNAs on chondrogenic differentiation. To investigate the mechanisms of miRNAs-mediated regulation during the process, we performed miRNAs microarray in MSCs at four different stages of TGF-β3-induced chondrogenic differentiation. We observed that eight miRNAs were significantly up-regulated and five miRNAs were downregulated. Interestingly, we found two miRNAs clusters, miR-143/145 and miR-132/212, kept on down-regulation in the process. Using bioinformatics approaches, we analyzed the target genes of these differentially expressed miRNAs and found a series of them correlated with the process of chondrogenesis. Furthermore, the qPCR results showed that the up-regulated (or down-regulated) expression of miRNAs were inversely associated with the expression of predicted target genes. Our results first revealed the expression profiles of miRNAs in chondrogenic differentiation of MSCs and provided a new insight on complicated regulation mechanisms of chondrogenesis.
Laboratory of Biomechanics, Department of Anatomy, the Third Military Medical University, Chongqing, P.R. China.
Bo
B
Yang
Hongfeng
H
Guo
Yulan
Y
Zhang
Shiwu
S
Dong
Dajun
D
Ying
D000818:Animals; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology; D020219:Chondrogenesis; D019295:Computational Biology; D020869:Gene Expression Profiling; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D053782:Transforming Growth Factor beta3 / Q000378:metabolism
D035683:MicroRNAs; D053782:Transforming Growth Factor beta3