Proteomic analysis of secreted proteins from aseptically grown rice.
Plants are known to secrete a variety of compounds into the rhizosphere. These compounds are thought to play important roles in the regulation of soil chemical properties and soil microorganisms. To determine the composition of proteins secreted from rice roots, aseptic hydro culture was performed, and the collected proteins were analyzed. Over 100 proteins were identified; most were identified using the rice database (RAP-DB), and about 60% of the identified proteins were suspected to have a signal peptide. Functional categorization suggested that most were secondary metabolism- and defense-related proteins. Pathogenesis- and stress-related proteins were the major proteins found in the bathing solution under aseptic conditions. Thus, we propose that rice plants constitutively secrete a large variety of proteins to protect their roots against abiotic and/or biotic stresses in the environment.
National Agriculture and Food Research Organization, National Agricultural Research Center for Hokkaido Region, 1-Hitsujigaoka, Toyohira-ku, Sapporo 062-8555, Japan. shinano@affrc.go.jp
T
T
Shinano
S
S
Komatsu
T
T
Yoshimura
S
S
Tokutake
F-J
FJ
Kong
T
T
Watanabe
J
J
Wasaki
M
M
Osaki
D000595:Amino Acid Sequence; D030541:Databases, Genetic; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012275:Oryza / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D058441:Rhizosphere; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010940:Plant Proteins