Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents.
Root colonization by arbuscular mycorrhizal (AM) fungi of Artemisia umbelliformis, investigated in natural and cultivated sites in the Southern Alps of France, showed typical structures (arbuscules, vesicles, hyphae) as well as spores and mycelia in its rhizosphere. Several native AM fungi belonging to different Glomeromycota genera were identified as colonizers of A. umbelliformis roots, including Glomus tenue, Glomus intraradices, G. claroideum/etunicatum and a new Acaulospora species. The use of the highly mycorrhizal species Trifolium pratense as a companion plant impacted positively on mycorrhizal colonization of A. umbelliformis under greenhouse conditions. The symbiotic performance of an alpine microbial community including native AM fungi used as inoculum on A. umbelliformis was evaluated in greenhouse conditions by comparison with mycorrhizal responses of two other alpine Artemisia species, Artemisia glacialis and Artemisia genipi Weber. Contrary to A. genipi Weber, both A. umbelliformis and A. glacialis showed a significant increase of P concentration in shoots. Volatile components were analyzed by GC-MS in shoots of A. umbelliformis 6 months after inoculation. The alpine microbial inoculum increased significantly the percentage of E-β-ocimene and reduced those of E-2-decenal and (E,E)-2-4-decadienal indicating an influence of alpine microbial inoculum on essential oil production. This work provides practical indications for the use of native AM fungi for A. umbelliformis field culture.
UMR INRA 1088/CNRS 5184/Université Bourgogne, Plante-Microbe-Environnement, INRA-CMSE, 17 rue Sully, BP 86510, 21065, Dijon Cedex, France. mnbinet@dijon.inra.fr.
Marie-Noëlle
MN
Binet
UMR INRA 1088/CNRS 5184/Université Bourgogne, Plante-Microbe-Environnement, INRA-CMSE, 17 rue Sully, BP 86510, 21065, Dijon Cedex, France.
Diederik
D
van Tuinen
Centre des Sciences du Goût et de l'Alimentation, INRA, 17 Rue Sully, 21000, Dijon, France.
Nicolas
N
Deprêtre
UMR INRA 1088/CNRS 5184/Université Bourgogne, Plante-Microbe-Environnement, INRA-CMSE, 17 rue Sully, BP 86510, 21065, Dijon Cedex, France.
Nathalie
N
Koszela
Centre de Recherche en Biotechnologie Végétale, CERBIOTECH, 117 Route de Veynes, 05000, Gap, France.
Catherine
C
Chambon
UMR INRA 1088/CNRS 5184/Université Bourgogne, Plante-Microbe-Environnement, INRA-CMSE, 17 rue Sully, BP 86510, 21065, Dijon Cedex, France.
Silvio
S
Gianinazzi
D019306:Artemisia / Q000378:metabolism* / Q000382:microbiology*; D005602:France; D055137:Glomeromycota / Q000502:physiology*; D038821:Mycorrhizae / Q000502:physiology*; D009822:Oils, Volatile / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D013559:Symbiosis
D009822:Oils, Volatile; D010710:Phosphates
Bioresour Technol. 2002 Jan;81(1):77-9
11708758
BMC Plant Biol. 2010 Jan 29;10:19
20113506
Mycorrhiza. 2006 Sep;16(6):443-446
16909287
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Mycorrhiza. 2007 Dec;18(1):1-14
17879101
New Phytol. 2009;182(2):347-358
19207688
Mycorrhiza. 2002 Feb;12(1):29-36
11968944
Environ Microbiol. 2001 Aug;3(8):525-31
11578313
Mycorrhiza. 2004 Apr;14(2):111-7
12768382
J Chem Ecol. 2009 Jul;35(7):833-43
19568812
Mol Ecol. 1998 Jul;7(7):879-87
9691489
J Ethnobiol Ethnomed. 2009 Nov 06;5:32
19895681
J Agric Food Chem. 2009 May 13;57(9):3436-43
19326948
Mycorrhiza. 2007 Oct;17(7):581-587
17578608
Mycologia. 2006 Mar-Apr;98(2):286-94
16894974
Mycorrhiza. 2006 Oct;16(7):485-494
16896796
New Phytol. 2009 Mar;181(4):938-949
19140934
Oecologia. 1980 Apr;45(1):57-62
28310937
Plant Biol (Stuttg). 2008 Jan;10(1):108-22
18211551