[Growth and differentiation effect on HL60 cells by adenovirus-mediated exogenous wnt5a gene modification on human bone marrow mesenchymal stem cells].
OBJECTIVE
To study the effect of human bone marrow mesenchymal stem cell (bMSCs) modified by the adenovirus-mediated exogenous wnt5a gene on the hematopoietic function of bone marrow and the inhibition of the growth of HL60 leukemia cells.


METHODS
BMSCs were identified through flow cytometry and modified by Ad5-wnt5a, The transfection rate of wnt5a gene in bMSCs was detected by RT-PCR. The cell growth curves were detected in different groups (bMSCs group, Ad5-GFP group, Ad5-wnt5a group). Ad5-wnt5a-bMSCs and HL60 cells were co-cultured, the surface differentiation antigen (CD13, CD14, CD68) and cell cycles were detected by immunocytochemical and flow cytometry in different groups (HL60 cell group, HL60+bMSCs group, HL60+ Ad5-GFP group, HL60+ Ad5-wnt5a group), respectively.


RESULTS
Adenovirus-mediated exogenous wnt5a gene was transfected into bMSCs. The expression of differentiation antigens of CD14, CD68 in HL60+Ad5-wnt5a group were higher than those in control group (P < 0.05), the expression of differentiation antigen CD13 were not significant difference in different groups (P > 0.05). Compared with control group, the cell cycle in HL60+ Ad5-wnt5a group was blocked at G2 phase in the fourth day.


CONCLUSIONS
Exogenous wnt5a gene can promote the growth of bMSCs, and induce HL60 cells to differentiation and maturation.
Laboratory of Children's Oncology, Institute of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
Ya-li
YL
Shen
You-hua
YH
Xu
Qing
Q
Luo
Zhen-hua
ZH
Guo
Gai-huan
GH
Zheng
Yu-xia
YX
Guo
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation; D018922:HL-60 Cells; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D051153:Wnt Proteins / Q000096:biosynthesis / Q000235:genetics*; D000071818:Wnt-5a Protein; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018950:Lipopolysaccharide Receptors; D011518:Proto-Oncogene Proteins; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C085389:WNT5A protein, human