Antigenotoxicity of artepillin C in vivo evaluated by the micronucleus and comet assays.
Artepillin C (3,5-diprenyl-p-coumaric acid), a major compound found in Brazilian green propolis and Baccharis dracunculifolia, shows anti-inflammatory, antibacterial, antiviral, antioxidant and antitumoral activities, among others. The aim of this study was to evaluate the genotoxic potential of artepillin C and its ability to prevent the chemically induced chromosome breakage or loss and the primary DNA damage using the micronucleus and comet assays in male Swiss mice, respectively. The animals were treated by gavage with different doses of artepillin C (0.4, 0.8 and 1.6 mg kg(-1) b.w.). For the antigenotoxicity assays, the different doses of artepillin C were administered simultaneously to doxorubicin (DXR; micronucleus test; 15 mg kg(-1) b.w.) and to methyl methanesulfonate (MMS; comet assay; 40 mg kg(-1) b.w.). The results showed that artepillin C itself was not genotoxic in the mouse micronucleus and comet assays. In the animals treated with artepillin C and DXR, the number of micronucleated reticulocytes was significantly lower in comparison with the animals treated only with DXR. Regarding antigenotoxicity, artepillin C at the tested doses significantly reduced the extent of DNA damage in liver cells induced by MMS.
Universidade de Franca, Avenida Dr Armando Salles de Oliveira, 201 Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
Moacir
M
de Azevedo Bentes Monteiro Neto
Ildercílio Mota
IM
de Souza Lima
Ricardo Andrade
RA
Furtado
Jairo Kenupp
JK
Bastos
Ademar Alves
AA
da Silva Filho
Denise Crispim
DC
Tavares
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D031175:Baccharis / Q000737:chemistry; D019457:Chromosome Breakage / Q000187:drug effects; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects*; D004317:Doxorubicin / Q000633:toxicity; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D010666:Phenylpropionates / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D012156:Reticulocytes / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D000998:Antiviral Agents; D009153:Mutagens; D010666:Phenylpropionates; D010936:Plant Extracts; C102259:artepillin C; D004317:Doxorubicin; D008741:Methyl Methanesulfonate