Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS.
BACKGROUND
parenteral zanamivir is a promising drug for the treatment of severe influenza. However, quantification of this polar drug in biological matrices has traditionally been difficult and the methods developed have been relatively insensitive.


RESULTS
a high-throughput bioanalytical method for the analysis of zanamivir in human plasma using SPE in the 96-well plate format and LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 50 microl of plasma and covers a large working range from 1-50, 000 ng/ml with a LOD of 0.50 ng/ml.


CONCLUSIONS
this new LC-MS/MS assay is more sensitive than previous methods despite using a small plasma volume sample. It is particularly suitable for clinical studies on both parenteral and inhaled zanamivir.
Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand. niklas@tropmedres.ac
Niklas
N
Lindegardh
Warunee
W
Hanpithakpong
Benjamas
B
Kamanikom
Jeremy
J
Farrar
Tran Tinh
TT
Hien
Pratap
P
Singhasivanon
Nicholas J
NJ
White
Nicholas P J
NP
Day
D000998:Antiviral Agents / Q000097:blood* / Q000627:therapeutic use; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007251:Influenza, Human / Q000188:drug therapy; D010949:Plasma; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D053243:Zanamivir / Q000097:blood* / Q000627:therapeutic use
D000998:Antiviral Agents; D053243:Zanamivir
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