Increase of lycopene production by supplementing auxiliary carbon sources in metabolically engineered Escherichia coli.
In the fed-batch culture of glycerol using a metabolically engineered strain of Escherichia coli, supplementation with glucose as an auxiliary carbon source increased lycopene production due to a significant increase in cell mass, despite a reduction in specific lycopene content. L-Arabinose supplementation increased lycopene production due to increases in cell mass and specific lycopene content. Supplementation with both glucose and L-arabinose increased lycopene production significantly due to the synergistic effect of the two sugars. Cell growth by the consumption of carbon sources was related to endogenous metabolism in the host E. coli. Supplementation with L-arabinose stimulated only the mevalonate pathway for lycopene biosynthesis and supplementation with both glucose and L-arabinose stimulated synergistically only the mevalonate pathway. In the fed-batch culture of glycerol with 10 gl(-1) glucose and 7.5 gl(-1) L-arabinose, the cell mass, lycopene concentration, specific lycopene content, and lycopene productivity after 34 h were 42 gl(-1), 1,350 mgl(-1), 32 mg g cells(-1), and 40 mgl(-1)h(-1), respectively. These values were 3.9-, 7.1-, 1.9-, and 11.7-fold higher than those without the auxiliary carbon sources, respectively. This is the highest reported concentration and productivity of lycopene.
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
Yeong-Su
YS
Kim
Jae-Hee
JH
Lee
Nam-Hee
NH
Kim
Soo-Jin
SJ
Yeom
Seon-Won
SW
Kim
Deok-Kun
DK
Oh
D001089:Arabinose / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism*; D002338:Carotenoids / Q000096:biosynthesis*; D018929:Cell Culture Techniques; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D000077276:Lycopene; D008798:Mevalonic Acid / Q000378:metabolism
D002338:Carotenoids; D002244:Carbon; D001089:Arabinose; D005947:Glucose; D008798:Mevalonic Acid; D000077276:Lycopene