Association of neighboring β-strands of outer membrane protein A in lipid bilayers revealed by site-directed fluorescence quenching.
We present a detailed study on the formation of neighboring β-strands during the folding of a monomeric integral membrane protein of the β-barrel type. β-Strand and β-barrel formations were investigated for the eight-stranded transmembrane domain of outer membrane protein A (OmpA) with single-tryptophan (W), single-cysteine (C) OmpA mutants. Based on the OmpA structure, W and C were introduced in two neighboring β-strands oriented toward the hydrocarbon core of the membrane. Replaced residue pairs were closer to either the periplasmic turns (named cis-side) or the outer loops (named trans-side) of the strand. W(n)C(m) OmpA mutants containing W at position n and C at position m along the polypeptide chain were labeled at the C by a nitroxyl spin label, which is a short-range fluorescence quencher. To monitor the association of neighboring β-strands, we determined the proximity between fluorescent W and labeled C in OmpA folding experiments by intramolecular fluorescence quenching. Formation of native β-strand contacts in folding experiments required the lipid membrane. Residues in the trans-side of strands β(1), β(2), and β(3), represented by mutants W(15)C(35) (β(1)β(2), trans) and W(57)C(35) (β(3)β(2), trans), reached close proximity prior to residues in the N(β(1))- and C(β(8))-terminal strands as examined for mutants W(15)C(162) (β(1)β(8), trans) and W(7)C(170) (β(1)β(8), cis). Tryptophan and cysteine converged slightly faster in W(15)C(162) (β(1)β(8), trans) than in W(7)C(170) (β(1)β(8), cis). The last folding step was observed for residues at the cis-ends of strands β(1) and β(2) for the mutant W(7)C(43) (β(1)β(2), cis). The data also demonstrate that the neighboring β-strands associate upon insertion into the hydrophobic core of the lipid bilayer.
Fachbereich Biologie, Universität Konstanz, Konstanz, Germany. joerg.helmut.kleinschmidt@uni-konstanz.de
Jörg H
JH
Kleinschmidt
Paula V
PV
Bulieris
Jian
J
Qu
Marileen
M
Dogterom
Tanneke
T
den Blaauwen
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D005453:Fluorescence; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D013113:Spin Labels; D014364:Tryptophan / Q000235:genetics / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D008051:Lipid Bilayers; D013113:Spin Labels; C078167:OMPA outer membrane proteins; D014364:Tryptophan