Distribution of human papillomavirus genotypes in sardinian patients with oral squamous cell carcinoma.
Human papillomaviruses (HPVs) seem to play an important role in the pathogenesis of gynecological carcinomas and in head and neck carcinomas. The aim of this study was to detect and genotype HPVs in fresh oral squamous cell carcinoma (OSCC) from a Sardinian population, and to determine whether HPV presence was significantly associated with the development of OSCC.The oral mucosa tissues were obtained from 120 samples (68 OSCC and 52 control samples) taken from a Sardinian population seen at the Dental Clinic of the Department of Surgery and Odontostomatological Sciences, University of Cagliari (Italy) and the " Ospedale SS Trinità", Cagliari (A.S.L. 8) between 2007 and 2008. PCR was used for the detection of HPV DNA and the genotype was determined by DNA sequencing. The frequency of HPV infection was evaluated in relation to age, sex, smoking and alcohol use. Statistical analysis was performed using the SPSS 11.5 software.The results showed the presence of HPV-DNA in 60.3% of OSCC with HPV-16 (51.2%) being the most frequent genotype. In these Sardinian OSCC patients, HPV-DNA was detected more in males (65.8%) than in females (34.1%) while controls show a 0% of HPV presence. HPV positive was highly associated with OSCC among subjects with a history of heavy tobacco and alcohol use and among those with no such history.A greater frequency of high risk HPV presence was observed in patients with OSCC compared to health control patients. In addition these results suggested that oral HPV presence could be associated in OSCC subjects. Our results need more analyses to detect the HPV-DNA integration into tumoral cells.
Surgery Department of Odontostomatological Sciences, Odontostomatology Section, O.B.L., University of Cagliari, Cagliari, Italy.
Caterina
C
Montaldo
Andrea
A
Mastinu
Stefania
S
Zorco
Noemi
N
Santini
Elisabetta
E
Pisano
Vincenzo
V
Piras
Gloria
G
Denotti
Carla
C
Peluffo
Matteo
M
Erriu
Valentino
V
Garau
Germano
G
Orrù
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 Jun;91(6):622-35
11402272
Int J Cancer. 1992 Oct 21;52(4):530-3
1399131
BMC Infect Dis. 2006 Jun 08;6:95
16762074
Afr J Med Med Sci. 2005 Mar;34(1):99-102
15971563
Oral Dis. 2007 Mar;13(2):239-43
17305629
Cancer Epidemiol Biomarkers Prev. 2005 Feb;14(2):467-75
15734974
Oral Dis. 2006 Sep;12(5):487-92
16910920
J Oral Pathol Med. 2005 Jan;34(1):17-22
15610402
Swed Dent J Suppl. 2005;(179):1-66
16335030
Oral Surg Oral Med Oral Pathol. 1988 May;65(5):545-50
2836773
Int J Oncol. 2007 Apr;30(4):813-23
17332919
Otolaryngol Head Neck Surg. 2001 Jul;125(1):1-9
11458206
Head Neck. 1997 May;19(3):200-4
9142519
J Oral Pathol Med. 2006 Jul;35(6):338-44
16762014
Virus Res. 2002 Nov;89(2):213-28
12445661
Curr Protoc Bioinformatics. 2002 Aug;Chapter 3:Unit 3.3
18792938
Hum Biol. 1991 Jun;63(3):253-72
2055588
JAMA. 2007 Feb 28;297(8):813-9
17327523
Sex Transm Dis. 2008 Mar;35(3):276-82
18091564
Laryngoscope. 2004 Nov;114(11):1906-9
15510012
Biochim Biophys Acta. 1996 Oct 9;1288(2):F55-78
8876633
J Natl Cancer Inst. 2003 Dec 3;95(23):1772-83
14652239
J Natl Cancer Inst. 2000 May 3;92(9):690-8
10793105
J Oral Pathol Med. 1992 Jul;21(6):265-9
1323673
J Oral Pathol Med. 1991 Aug;20(7):305-17
1654422
J Clin Microbiol. 1997 Jun;35(6):1304-10
9163434
Ann Surg Oncol. 2003 Jun;10(5):551-7
12794022
Rev Med Inst Mex Seguro Soc. 2006 Mar-Apr;44(2):147-53
16674861
Methods Mol Med. 2005;119:349-62
16350410
J Natl Cancer Inst. 2000 May 3;92(9):709-20
10793107
Cancer. 1997 Nov 1;80(9):1803-4
9351551
J Clin Microbiol. 1999 Aug;37(8):2508-17
10405393
J Virol Methods. 2000 Aug;88(2):125-34
10960700
Oral Surg Oral Med Oral Pathol. 1986 Dec;62(6):660-7
3025793
Arch Pathol Lab Med. 2003 Aug;127(8):940-5
12873165
Vaccine. 2008 Aug 19;26 Suppl 12:M1-16
18945410
J Oral Pathol Med. 1991 May;20(5):210-4
1648613
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996 Jul;82(1):57-68
8843455
Eur J Cancer B Oral Oncol. 1996 Jan;32B(1):55-62
8729620
Head Neck. 1998 May;20(3):250-65
9570632
Arch Med Res. 2008 Feb;39(2):189-97
18164962
J Infect Dis. 2002 Mar 15;185(6):833-6
11920302
Int J Cancer. 2004 Feb 20;108(5):766-72
14696105
Quintessence Int. 2006 Oct;37(9):707-11
17017632
J Oral Pathol Med. 2007 Sep;36(8):482-7
17686007
J Oral Pathol Med. 2006 Feb;35(2):86-90
16430738
Am J Manag Care. 2006 Dec;12(17 Suppl):S462-72
17203990
Proc Nutr Soc. 2006 Aug;65(3):319-25
16923315
Gynecol Oncol. 2005 Jan;96(1):84-91
15589585
Braz J Otorhinolaryngol. 2006 Mar-Apr;72(2):272-82
16951865
Int J Gynecol Cancer. 2007 Nov-Dec;17(6):1307-13
17425678
Eur J Cancer. 2007 May;43(7):1188-99
17383866
Ann Otol Rhinol Laryngol. 2003 Apr;112(4):298-302
12731623
Clin Cancer Res. 2002 Oct;8(10):3187-92
12374687
Rinsho Byori. 2007 Oct;55(10):922-9
18050669
Crit Rev Oral Biol Med. 2004 Jul 01;15(4):188-96
15284184
Mod Pathol. 2000 Jun;13(6):644-53
10874669
J Clin Microbiol. 2007 Mar;45(3):928-34
17229855
Proc Finn Dent Soc. 1983;79(1):1-8
6306646
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 May;95(5):594-600
12738951