Corticotropin-releasing hormone inhibits in vitro oocyte maturation in mice.
The expression of corticotropin-releasing hormone (CRH) receptor 1 messenger RNA in stages of follicle growth was examined by reverse transcriptase-polymerase chain reaction in long-term cultures of early preantral mouse follicles with and without CRH addition. Corticotropin-releasing hormone receptor 1 is present in stages of mouse follicle growth, whereas 10(-9), 10(-7), and 10(-6) mol/L CRH inhibits oocyte maturation in vitro, an effect reversed by antalarmin addition.
1st Department of Obstetrics and Gynecology, Alexandra Hospital, University of Athens, Athens, Greece. ekiapek@otenet.gr
Erasmia
E
Kiapekou
Evangelia
E
Zapanti
Dionysia
D
Voukelatou
Theodora
T
Mavreli
Konstantinos
K
Stefanidis
Peter
P
Drakakis
George
G
Mastorakos
Dimitris
D
Loutradis
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D005260:Female; D006131:Growth Inhibitors / Q000502:physiology*; D006727:Hormone Antagonists / Q000494:pharmacology; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D009866:Oogenesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000096:biosynthesis
D006131:Growth Inhibitors; D006727:Hormone Antagonists; D018019:Receptors, Corticotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone