Manassantin A and B from Saururus chinensis inhibit interleukin-6-induced signal transducer and activator of transcription 3 activation in Hep3B cells.
Inhibition of interleukin-6 (IL-6) has been postulated to be an effective therapy in the pathogenesis of several inflammatory diseases. The current study was performed to examine potential effects of manassantin A and B isolated from Saururus chinensis on the IL-6-induced response to human hepatoma cells. We found that manassantin A and B inhibit signal transducer and activator of transcription 3 (Stat3) activity stimulated by IL-6. We also found that both compounds decreased IL-6-induced Stat3 phosphorylation and nuclear translocation. Both compounds blocked suppressor of cytokine signaling 3 (SOCS-3)-mRNA expression induced by IL-6. In addition, we found that Stat3 inhibitory effects of these compounds could be related to protein tyrosine phosphatase. These findings suggest that manassantin A and B could be useful remedies for treatment of inflammatory diseases by inhibiting IL-6 action.
Eco-friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk, Republic of Korea.
Jong Sun
JS
Chang
Seung Woong
SW
Lee
Myo Sun
MS
Kim
Bo Ra
BR
Yun
Mi Hye
MH
Park
Seok-Geun
SG
Lee
Su-Jin
SJ
Park
Woo Song
WS
Lee
Mun-Chual
MC
Rho
D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology; D015850:Interleukin-6 / Q000502:physiology; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D010766:Phosphorylation / Q000187:drug effects; D017027:Protein Tyrosine Phosphatases / Q000502:physiology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D032134:Saururaceae / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D000093242:Interleukin-6 Inhibitors
D005663:Furans; D015850:Interleukin-6; D017705:Lignans; D017027:Protein Tyrosine Phosphatases; D050796:STAT3 Transcription Factor; D000093242:Interleukin-6 Inhibitors; C494086:STAT3 protein, human; C482885:manassantin B; C054895:manassantin A