Applications of a new In vivo tumor spheroid based shell-less chorioallantoic membrane 3-D model in bioengineering research.
The chicken chorioallantoic membrane (CAM) is a classical in vivo biological model in studies of angiogenesis. Combined with the right tumor system and experimental configuration this classical model can offer new approaches to investigating tumor processes. The increase in development of biotechnological devices for cancer diagnosis and treatment, calls for more sophisticated tumor models that can easily adapt to the technology, and provide a more accurate, stable and consistent platform for rapid quantitative and qualitative analysis. As we discuss a variety of applications of this novel in vivo tumor spheroid based shell-less CAM model in biomedical engineering research, we will show that it is extremely versatile and easily adaptable to an array of biomedical applications. The model is particularly useful in quantitative studies of the progression of avascular tumors into vascularized tumors in the CAM. Its environment is more stable, flat and has a large working area and wider field of view excellent for imaging and longitudinal studies. Finally, rapid data acquisition, screening and validation of biomedical devices and therapeutics are possible with the short experimental window.
Department of Biomedical Engineering, University of California, Irvine, USA.
Nzola
N
De Magalhães
Lih-Huei L
LH
Liaw
Michael
M
Berns
Vittorio
V
Cristini
Zhongping
Z
Chen
Dwayne
D
Stupack
John
J
Lowengrub
Anat Rec. 2001 Dec 1;264(4):317-24
11745087
Biophys J. 2005 Dec;89(6):3884-94
16199495
Clin Transl Oncol. 2008 Feb;10(2):85-95
18258507
Poult Sci. 1974 Jan;53(1):409-12
4833046
Curr Opin Ophthalmol. 1999 Jun;10(3):182-8
10537777
Pathobiology. 1999 May-Jun;67(3):148-57
10394136
Opt Express. 2002 Mar 11;10(5):236-45
19436351
J Math Biol. 2009 Apr;58(4-5):723-63
18787827
Methods Mol Med. 2003;78:311-5
12825280
Photochem Photobiol Sci. 2002 Sep;1(9):721-8
12665312
Lancet. 1958 Jun 7;1(7032):1188-95
13550965
J Math Biol. 2009 Apr;58(4-5):765-98
18781303
Cancer Res. 2006 Feb 1;66(3):1597-604
16452218
Opt Lett. 2008 Jul 1;33(13):1425-7
18594653
J Math Biol. 2009 Apr;58(4-5):485-510
18781304
J Theor Biol. 2008 Aug 7;253(3):524-43
18485374
Pharmacol Toxicol. 1989 Feb;64(2):193-5
2474162
Neuroimage. 2007;37 Suppl 1:S59-70
17475515
J Photochem Photobiol B. 1999 Nov-Dec;53(1-3):44-52
10672528
Biomed Microdevices. 2004 Dec;6(4):297-309
15548877
J Math Biol. 2004 Aug;49(2):111-87
15293017
Lasers Surg Med. 2006 Jun;38(5):555-64
16788918
Eur J Pharm Biopharm. 2008 May;69(1):43-53
18023564
Mol Carcinog. 2007 Oct;46(10):872-85
17455221
Opt Lett. 1997 Jul 15;22(14):1119-21
18185770
Cancer Res. 2009 May 15;69(10):4493-501
19366801
Nat Methods. 2007 Oct;4(10):855-60
17767164
Cancer Res. 2009 Mar 1;69(5):1712-6
19223530
Methods Enzymol. 2008;443:65-82
18772011
Photochem Photobiol. 2000 Jul;72(1):128-34
10911737
Methods Mol Biol. 2000;135:39-46
10791303
Hum Cell. 1999 Mar;12(1):37-46
10457904
Dermatology. 1999;198(4):355-61
10449934
Adv Drug Deliv Rev. 2007 Sep 30;59(11):1162-76
17870202
Microcirculation. 2008 Jan;15(1):65-79
17952797
Int J Exp Pathol. 2005 Dec;86(6):365-74
16309542
J Theor Biol. 2007 Apr 21;245(4):677-704
17239903