[Construction of recombinant adenovirus carrying HBx and mIL-12].
OBJECTIVE
To construct a recombinant adenovirus Ad-HBx-mlL-12 carrying HBx and mIL-12.


METHODS
HBx and mIL-12 were cloned into the shuttle plasmid pAdenoVator-CMV5 and confirmed by means of enzymatic manipulation. After linearization by EcoR I digestion, the recombinant shuttle plasmid pAdV-HBx-mIL-12 was transformed into competent BJ5183 germs with the adenoviral backbone plasmid pAdenoVator deltaE1/E3 and then homologically recombined to obtain the recombinant adenovirus plasmid. After confirmation, the recombinant adenovirus plasmid pAd-HBx-mIL-12 was linearized with Pac I digestion and transfected into 293 cells via liposome, and then adenovirus package and amplification were performed.


RESULTS
It was confirmed that the recombinant adenovirus Ad-HBx-mIL-12 had been successfully constructed and both HBx and mIL-12 were expressed in 293 cells.


CONCLUSIONS
The recombinant adenovirus carrying HBx and mIL-12 has been successfully constructed, which lays a foundation for the further study of antitumor mechanism and gene therapy.
Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
Ya-ou
YO
Li
Ping
P
Chen
Zhi-yong
ZY
Li
Yan-jun
YJ
Wen
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000628:therapy; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins
D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D018664:Interleukin-12