Effects of heat treatment on hydrogen production potential and microbial community of thermophilic compost enrichment cultures.
Cellulosic plant and waste materials are potential resources for fermentative hydrogen production. In this study, hydrogen producing, cellulolytic cultures were enriched from compost material at 52, 60 and 70°C. Highest cellulose degradation and highest H(2) yield were 57% and 1.4 mol-H(2) mol-hexose(-1) (2.4 mol-H(2) mol-hexose-degraded(-1)), respectively, obtained at 52°C with the heat-treated (80°C for 20 min) enrichment culture. Heat-treatments as well as the sequential enrichments decreased the diversity of microbial communities. The enrichments contained mainly bacteria from families Thermoanaerobacteriaceae and Clostridiaceae, from which a bacterium closely related to Thermoanaerobium thermosaccharolyticum was mainly responsible for hydrogen production and bacteria closely related to Clostridium cellulosi and Clostridium stercorarium were responsible for cellulose degradation.
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland. marika.nissila@tut.fi
Marika E
ME
Nissilä
Hanne P
HP
Tähti
Jukka A
JA
Rintala
Jaakko A
JA
Puhakka
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis*; D057919:Biological Oxygen Demand Analysis; D002482:Cellulose / Q000378:metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D006358:Hot Temperature; D006859:Hydrogen / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D056804:Biofuels; D012336:RNA, Ribosomal, 16S; D012987:Soil; D006859:Hydrogen; D002482:Cellulose