Genomic analysis of bacteriophage ϕAB1, a ϕKMV-like virus infecting multidrug-resistant Acinetobacter baumannii.
We present the complete genomic sequence of a lytic bacteriophage ϕAB1 which can infect many clinical isolates of multidrug-resistant Acinetobacter baumannii. The recently isolated bacteriophage displays morphology resembling Podoviridae family. The ϕAB1 genome is a linear double-stranded DNA of 41,526 bp containing 46 possible open reading frames (ORFs). The majority of the predicted structural proteins were identified as part of the phage particle by mass spectrometry analysis. According to the virion morphology, overall genomic structure, and the phylogenetic tree of RNA polymerase, we propose that ϕAB1 is a new member of the ϕKMV-like phages. Additionally, we identified four ORFs encoding putative HNH endonucleases, one of which is presumed to integrate and create a genes-in-pieces DNA polymerase. Also, a potential lysis cassette was identified in the late genome. The lytic power of this bacteriophage combined with its specificity for A. baumannii makes ϕAB1 an attractive agent for therapeutic or disinfection applications.
Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970, Taiwan.
Kai-Chih
KC
Chang
Nien-Tsung
NT
Lin
Anren
A
Hu
Yu-Shan
YS
Lin
Li-Kuang
LK
Chen
Meng-Jiun
MJ
Lai
D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000821:virology*; D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004720:Endonucleases / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017902:Podoviridae / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases; D004720:Endonucleases