Fecal dehydroepiandrosterone (DHEA) immunoreactivity as a noninvasive index of circulating DHEA activity in young male laboratory rats.
Evidence suggests that dehydroepiandrosterone (DHEA) plays a key role in stress and coping responses. Fecal sampling permits assessment of hormone-behavior interactions reliably and effectively, but no previous study has compared circadian- or stress-dependent alterations between serum DHEA and its fecal metabolites. In the current study, young (28 d of age) male rats were assigned to either an experimental (n = 6) or control (n = 6) group. Rats in the experimental group were exposed to a forced swim test to assess their behavioral and physiologic response to an environmental stressor; blood samples were drawn before the test (baseline), immediately after the test, and at 2 later time points. Only fecal samples were collected from control animals. Fecal DHEA and corticosterone metabolites were monitored in all animals for 24 h. DHEA metabolites in control rats exhibited significant diurnal variation, showing a similar temporal pattern as that of corticosterone metabolites. In addition, fecal and serum DHEA levels were highly correlated. Significant peaks in both DHEA and corticosterone metabolite levels were detected. These data suggest that measures of fecal DHEA can provide a complementary, noninvasive method of assessing adrenal gland function in rats.
Department of Psychology, Marshall University, Huntington, West Virginia, USA. bardi@marshall.edu
Massimo
M
Bardi
Joseph E
JE
Hampton
Kelly G
KG
Lambert
D000223:Adaptation, Psychological; D000818:Animals; D002940:Circadian Rhythm; D003345:Corticosterone / Q000032:analysis* / Q000097:blood / Q000276:immunology; D003687:Dehydroepiandrosterone / Q000032:analysis* / Q000097:blood / Q000276:immunology; D005243:Feces / Q000737:chemistry*; D008297:Male; D051381:Rats / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D020318:Rats, Long-Evans; D013312:Stress, Physiological; D013550:Swimming
D003687:Dehydroepiandrosterone; D003345:Corticosterone
Neuropsychopharmacology. 2004 Aug;29(8):1546-57
15199367
Brain Behav Evol. 2007;70(4):218-26
17914253
Neuroscience. 2009 Mar 17;159(2):514-25
19361476
J Neuroendocrinol. 2008 Jun;20(6):880-4
18601712
Front Neuroendocrinol. 2009 Jan;30(1):65-91
19063914
Neurosci Biobehav Rev. 2006;30(4):497-510
16253328
J Steroid Biochem Mol Biol. 2008 Nov;112(1-3):138-44
18848627
Int J Neuropsychopharmacol. 2007 Apr;10(2):203-9
16674834
Reprod Domest Anim. 2008 Jul;43 Suppl 2:74-82
18638107
Neuroscience. 2009 Feb 18;158(4):1644-51
19068226
Immunobiology. 2010 Dec;215(12):980-6
20163889
Brain Res Rev. 2008 Mar;57(2):571-85
18164765
Physiol Biochem Zool. 2005 Nov-Dec;78(6):1069-84
16228945
Eur J Pharmacol. 2008 Apr 7;583(2-3):255-62
18339373
Neuroimmunomodulation. 2008;15(4-6):241-50
19047801
Biol Psychol. 2008 Dec;79(3):294-8
18706968
Am J Primatol. 2005 Sep;67(1):1-4
16163717
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4704-7
6458035
In Vivo. 2009 May-Jun;23(3):381-6
19454502
Primates. 2002 Jan;43(1):29-39
12091745
Behav Brain Res. 2008 Jun 26;190(1):33-40
18342957
Exp Physiol. 2004 Jul;89(4):427-33
15131075
Am J Psychiatry. 2004 Feb;161(2):195-216
14754765
Lab Anim. 2007 Jul;41(3):372-87
17640465
Neurosci Biobehav Rev. 1999 Nov;23(7):925-35
10580307
Physiol Biochem Zool. 2009 May-Jun;82(3):291-301
19335228
Steroids. 2010 Mar;75(3):213-8
19961867
J Vis Exp. 2007;(7):266
18989437
Neurosci Behav Physiol. 2009 Sep;39(7):695-9
19621272
Ann N Y Acad Sci. 2008 Dec;1148:495-503
19120147
Stress. 2010 Mar;13(2):172-83
20214438
Biol Psychiatry. 2009 Aug 15;66(4):334-40
19500775
Endocr Rev. 1980 Fall;1(4):392-410
6265199
Am J Phys Anthropol. 1998;Suppl 27:1-23
9881521
J Appl Toxicol. 1984 Oct;4(5):258-60
6512162
Lab Anim. 1995 Jul;29(3):250-7
7564207
Lab Anim. 2001 Oct;35(4):307-14
11669313
Arch Gen Psychiatry. 2000 Oct;57(10):925-35
11015810
Physiol Behav. 2008 Sep 3;95(1-2):187-93
18586285
Horm Behav. 2004 Jan;45(1):10-22
14733887
Psychoneuroendocrinology. 2010 Aug;35(7):1055-62
20153584
In Vivo. 2008 Jul-Aug;22(4):435-40
18712168