Determination of bavachin and isobavachalcone in Fructus Psoraleae by high-performance liquid chromatography with electrochemical detection.
A simple, sensitive and selective method of high-performance liquid chromatography with electrochemical detection (HPLC-ECD) has been developed for simultaneous determination of bavachin and isobavachalcone in Fructus Psoraleae. At optimized conditions, bavachin and isobavachalcone could be well separated within 15 min at a detection potential of +0.80 V with 0.03 mol/L acetate buffer solution (pH 5.17)/acetonitrile (2:3, v/v) as the mobile phase. The relationships between peak areas and concentrations were linear from 8.26 × 10(-7) to 1.21 × 10(-4) mol/L for bavachin, and from 1.01 × 10(-8) to 1.61 × 10(-4) mol/L for isobavachalcone, respectively. The method offered excellent linearity with regression coefficient R(2) >0.995. The method presented detection limits (S/N = 3) of 8.81 × 10(-9) mol/L for bavachin and 1.17 × 10(-10) mol/L for isobavachalcone. It indicates that the sensitivity of electrochemical detection is ten times higher than that of diode array detection (DAD). The mean recoveries around 98% with a relative standard deviation less than 3.1% for the two analytes have been obtained. The proposed separation and detection procedures were successfully applied to the simultaneous determination of bavachin and isobavachalcone in traditional Chinese medicine.
Institute of Pharmaceutical Analysis, College of Pharmacy, Wuhan University, Wuhan, PR China.
Yuan
Y
Li
Fang
F
Wang
Zilin
Z
Chen
D047188:Chalcones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D057230:Limit of Detection; D010936:Plant Extracts / Q000032:analysis*
D047188:Chalcones; D005419:Flavonoids; D010936:Plant Extracts; C459212:bavachin; C468754:isobavachalcone