[Effects of recombinant human erythropoietin on serum levels of neuron-specific enolase, S-100β protein and myelin basic protein in rats following status epilepticus].
OBJECTIVE
This study examined the effect of recombinant human erythropoietin (r-HuEPO) on the serum levels of neuron-specific enolase (NSE), S-100β protein and myelin basic protein (MBP) in young rats 24 hrs after lithium-pilocarpine-induced status epilepticus (SE) in order to study the potential role of r-HuEPO in epileptic brain damage.


METHODS
Forty 19-21-day-old male Sprague-Dawley (SD) rats were randomly divided into four groups (n=10): normal control group, SE, r-HuEPO pretreated-SE and r-HuEPO. SE was induced by lithium-pilocarpine. R-HuEPO (500 IU/kg) was intraperitoneally injected in the r-HuEPO pretreated-SE and r-HuEPO groups 4 hrs before SE. Serum levels of NSE, S-100β and MBP were determined 24 hrs after the SE event.


RESULTS
Serum levels of NSE, S-100β and MBP in the SE group increased significantly compared with those in the normal control and the r-HuEPO groups (P＜0.05). The r-HuEPO pretreated-SE group showed significantly decreased serum levels of NSE, S-100β and MBP compared with the SE group (P＜0.05).


CONCLUSIONS
r-HuEPO may reduce the expression of NSE, S-100β and MBP and thus might provide an early protective effect against epileptic brain injury.
Department of Pediatrics, Hangzhou People's Hospital Affiliated to Nanjing Medical University, Hangzhou 310006, China.
Chun-Ming
CM
Jiang
Jian-Min
JM
DU
Zhan-Li
ZL
Liu
Li-Qiong
LQ
Chen
Mei
M
Feng
Yi-Hua
YH
Yang
Jie-Hua
JH
Wang
Xian-Mei
XM
Huang
D000818:Animals; D004921:Erythropoietin / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D004676:Myelin Basic Protein / Q000097:blood*; D009414:Nerve Growth Factors / Q000097:blood*; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood*; D013226:Status Epilepticus / Q000097:blood / Q000188:drug therapy*
D004676:Myelin Basic Protein; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D004921:Erythropoietin; D010751:Phosphopyruvate Hydratase