In vitro antifungal susceptibility pattern and ergosterol content in clinical yeast strains.
BACKGROUND
Absence or severe reduction in the amount of ergosterol in the fungal membrane and its replacement with other sterols have been described as potential antifungal resistance mechanisms in fungi.


OBJECTIVE
The ergosterol content in a collection of 51 clinical yeast isolates, including susceptible and resistant strains to amphotericin B and azoles, was estimated by a simple chromatographic method (HPLC-UV).


RESULTS
A high content of ergosterol was detected for several strains of Candida glabrata, Candida tropicalis or Pichia membranifaciens. In contrast, strains of Cryptococcus neoformans and Dipodascus capitatus had the lowest ergosterol concentrations. No significant correlation was observed between antifungal susceptibility patterns and ergosterol content.


CONCLUSIONS
We conclude from this study that ergosterol content on yeasts may not be associated with specific resistant patterns.
Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, España. aliciagl@isciii.es
Alicia
A
Gomez-Lopez
María Jose
MJ
Buitrago
Juan Luis
JL
Rodriguez-Tudela
Manuel
M
Cuenca-Estrella
D000935:Antifungal Agents / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D004875:Ergosterol / Q000096:biosynthesis*; D006801:Humans; D008826:Microbial Sensitivity Tests; D015003:Yeasts / Q000187:drug effects* / Q000378:metabolism*
D000935:Antifungal Agents; D004875:Ergosterol