Differentiating atherosclerotic plaque burden in arterial tissues using femtosecond CARS-based multimodal nonlinear optical imaging.
A femtosecond CARS-based nonlinear optical microscope was used to simultaneously image extracellular structural proteins and lipid-rich structures within intact aortic tissue obtained from myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits (WHHLMI). Clear differences in the NLO microscopic images were observed between healthy arterial tissue and regions dominated by atherosclerotic lesions. In the current ex-vivo study, we present a single parameter based on intensity changes derived from multi-channel NLO image to classify plaque burden within the vessel. Using this parameter we were able to differentiate between healthy regions of the vessel and regions with plaque, as well as distinguish plaques relative to the age of the WHHLMI rabbit.
Leila B
LB
Mostaço-Guidolin
Michael G
MG
Sowa
Andrew
A
Ridsdale
Adrian F
AF
Pegoraro
Michael S D
MS
Smith
Mark D
MD
Hewko
Elicia K
EK
Kohlenberg
Bernie
B
Schattka
Masashi
M
Shiomi
Albert
A
Stolow
Alex C-T
AC
Ko
Am J Cardiol. 2006 Dec 18;98(12A):3Q-9Q
17169627
Nat Biotechnol. 2003 Nov;21(11):1369-77
14595365
Eur Heart J. 2008 Aug;29(16):2023
18337236
Opt Express. 2010 Feb 1;18(3):2380-8
20174068
Arterioscler Thromb Vasc Biol. 2003 Jul 1;23(7):1239-44
12738683
N Engl J Med. 2005 Apr 21;352(16):1685-95
15843671
Circulation. 2005 Mar 29;111(12):1551-5
15781733
Arteriosclerosis. 1983 Jan-Feb;3(1):87-101
6824499
J Biomed Opt. 2007 Sep-Oct;12(5):054007
17994895
Biophys J. 2005 Feb;88(2):1377-86
15533922
J Am Coll Cardiol. 1993 Jan;21(1):35-44
8417074
AJR Am J Roentgenol. 2008 Mar;190(3):748-54
18287448
J Atheroscler Thromb. 2004;11(4):184-9
15356377
Nat Biotechnol. 2003 Nov;21(11):1356-60
14595363
Biophys J. 2004 Oct;87(4):2778-86
15454469
Opt Lett. 2009 Aug 1;34(15):2249-51
19649060
Opt Express. 2007 Apr 2;15(7):4054-65
19532649
Opt Express. 2009 Feb 16;17(4):2984-96
19219203
Nat Methods. 2005 Dec;2(12):932-40
16299478
Arterioscler Thromb Vasc Biol. 2000 Feb;20(2):478-83
10669646
J Biomed Opt. 2010 Mar-Apr;15(2):020501
20459215
Biomed Eng Online. 2003 May 17;2:13
12801419
Arterioscler Thromb Vasc Biol. 2009 Sep;29(9):1342-8
19520975
Anal Chem. 2009 Feb 15;81(4):1496-504
19152314
Circulation. 1998 Feb 3;97(4):332-9
9468206
Circulation. 2008 Jul 29;118(5):586-606
18586979
Circulation. 2008 Apr 15;117(15):2024-37
18413510
J Biomed Opt. 2007 Jul-Aug;12(4):044005
17867809
J Biomed Opt. 2006 Mar-Apr;11(2):021003
16674178
Arteriosclerosis. 1987 Mar-Apr;7(2):113-24
3579721
J Lipid Res. 2003 Nov;44(11):2202-8
12923234
Nature. 2000 Sep 14;407(6801):233-41
11001066
Photomed Laser Surg. 2007 Dec;25(6):482-6
18158749
Eur Heart J. 2008 Mar;29(5):583-93
18238930
Med J Aust. 2008 Jun 16;188(12):728
18558898
Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7075-80
12756303
Appl Opt. 2006 Oct 20;45(30):7938-43
17068531
J Biomed Opt. 2009 May-Jun;14(3):034005
19566298
Basic Res Cardiol. 1994 Sep-Oct;89(5):397-410
7535519
Circulation. 1995 Sep 1;92(5):1355-74
7648691
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11014-9
12177437
EuroIntervention. 2009 Jan;4(4):529-33
19284077
Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16807-12
16263923
Opt Commun. 2008 Apr 1;281(7):1813-1822
19343073
J Biomed Opt. 2005 May-Jun;10(3):031117
16229642