WT1-specific CTL cells of recipient origin may exist in the peripheral blood of patients achieving full donor chimerism soon after nonmyeloablative transplantation.
This study was performed to assay whether leukemia-associated antigen (LAA)-specific CTLs of recipient origin existed in the blood of patients who achieved full donor chimerism (FDC) soon after nonmyeloablative transplantation (NST). In 15 patients who received haplo-identical NST, WT1(+) CD8(+) CTLs were detected with WT1/HLA-A*0201 pentamer, and the donor-recipient chimerism levels were analyzed by three methods. Results showed that WT1(+) CD8(+) CTLs could be detected in patients with HLA-A*0201 expressing only in recipient, and cells of recipient origin existed in the blood of patients who achieved FDC, which suggested that LAA-specific CTLs of recipient origin may exist in patients achieving FDC soon after NST.
Third Department of Internal Medicine, General Hospital of Tibet Military Region, Lhasa, China.
Li
L
Wei
Hongli
H
Zuo
Xuedong
X
Sun
Tieqiang
T
Liu
Mei
M
Guo
Guangxian
G
Liu
Qiyun
Q
Sun
Jianhui
J
Qiao
Danhong
D
Wang
Changlin
C
Yu
Kaixun
K
Hu
Zheng
Z
Dong
Huisheng
H
Ai
D000293:Adolescent; D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002648:Child; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000276:immunology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012730:Sex Chromosomes / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014019:Tissue Donors; D018183:Transplantation Chimera / Q000276:immunology*; D014184:Transplantation, Homologous; D025721:WT1 Proteins / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
D004273:DNA, Neoplasm; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D025721:WT1 Proteins