[Generation of dopamine neurons from human embryonic stem cells in vitro].
The aim of the study was to generate dopaminergic (DA) neurons from human embryonic stem cells (ESC) in vitro. It was shown that human ESCs are able to differentiated into DA neurons without co-culture with stromal cells. Terminal differentiation into DA neurons was reached by successive application of noggin and bFGF growth factors on collagen and matrigel substrates during 3-4 weeks. Differentiation efficiency was evaluated by the number of colonies with cells expressing tyrosine hydroxylase (TH), a DA neuron marker, and by the number of TH-positive cells in cell suspension using flow cytometry. No cells with pluripotent markers were detected in DA-differentiated cultures. It makes possible to propose that the protocol of human ESC differentiation might be applied to generate DA neurons for their transplantation into the animals modeling neurodegenerative (Parkinson) disease without the risk of tumor growth.
I V
IV
Kozhukharova
I I
II
Fridlianskaia
V I
VI
Zemel'ko
Z V
ZV
Kovaleva
N A
NA
Pugovkina
L L
LL
Alekseenko
M V
MV
Kharchenko
N D
ND
Aksenov
A N
AN
Shatrova
T M
TM
Grinchuk
S V
SV
Anisimov
N N
NN
Nikol'skiĭ
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002352:Carrier Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D004195:Disease Models, Animal; D004298:Dopamine; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D010300:Parkinson Disease / Q000378:metabolism / Q000628:therapy; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis; D000099176:Noggin Protein
D000943:Antigens, Differentiation; D002352:Carrier Proteins; D004298:Dopamine; D016222:Fibroblast Growth Factor 2; D014446:Tyrosine 3-Monooxygenase; D000099176:Noggin Protein