Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA.
Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over a wide range of up to 100 pN. We demonstrate the use of the method by overstretching of a short DNA and unfolding/refolding a protein of filamin A immunoglobulin domains 1-8. Other potential applications of this method are also discussed.
Mechanobiology Institute, National University of Singapore, Singapore.
Hu
H
Chen
Hongxia
H
Fu
Xiaoying
X
Zhu
Peiwen
P
Cong
Fumihiko
F
Nakamura
Jie
J
Yan
D002138:Calibration; D003285:Contractile Proteins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D064448:Filamins; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D008280:Magnetics / Q000295:instrumentation / Q000379:methods*; D008840:Microfilament Proteins / Q000737:chemistry*; D052898:Optical Tweezers; D017510:Protein Folding
D003285:Contractile Proteins; D064448:Filamins; D007128:Immunoglobulin Fragments; D008840:Microfilament Proteins; D004247:DNA
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