A biopsy is not always necessary to diagnose celiac disease.
OBJECTIVE
Small intestinal histology is the criterion standard for the diagnosis of celiac disease (CD). However, results of serological tests such as anti-endomysium antibodies and anti-tissue transglutaminase antibodies (tTGA) are becoming increasingly reliable. This raises the question of whether a small intestinal biopsy is always necessary. The aim of the present study was, therefore, to investigate whether a small intestinal biopsy can be avoided in a selected group of patients.


METHODS
Serology and histological slides obtained from 283 pediatric patients suspected of having CD were examined retrospectively. The response to a gluten-free diet (GFD) in patients with a tTGA level ≥ 100 U/mL was investigated.


RESULTS
A tTGA level ≥ 100 U/mL was found in 128 of the 283 patients. Upon microscopic examination of the small intestinal epithelium, villous atrophy was found in 124 of these patients, confirming the presence of CD. Three patients had crypt hyperplasia or an increased number of intraepithelial lymphocytes. In 1 patient no histological abnormalities were found. This patient did not respond to a GFD.


CONCLUSIONS
Pediatric patients with a tTGA level ≥ 100 U/mL in whom symptoms improve upon consuming a GFD may not need a small intestinal biopsy to confirm CD.
Department of Pediatric Gastroenterology, University Medical Center Utrecht, The Netherlands. A.Mubarak@umcutrecht.nl
Amani
A
Mubarak
Victorien M
VM
Wolters
Susan A M
SA
Gerritsen
Frits H J
FH
Gmelig-Meyling
Fiebo J W
FJ
Ten Kate
Roderick H J
RH
Houwen
D000293:Adolescent; D001284:Atrophy; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001706:Biopsy / Q000379:methods*; D002446:Celiac Disease / Q000175:diagnosis* / Q000178:diet therapy / Q000276:immunology / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D055050:Diet, Gluten-Free; D005260:Female; D006801:Humans; D006965:Hyperplasia; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology*; D007421:Intestine, Small / Q000276:immunology / Q000473:pathology*; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D018579:Patient Selection; D011503:Transglutaminases / Q000276:immunology*
D001323:Autoantibodies; D015415:Biomarkers; D011503:Transglutaminases