Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe.
We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.
Department of Chemistry, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA.
Carolina D
CD
Andrade
Ciceron O
CO
Yanez
Maher A
MA
Qaddoura
Xuhua
X
Wang
Curtesa L
CL
Arnett
Sabrina A
SA
Coombs
Jin
J
Yu
Rania
R
Bassiouni
Mykhailo V
MV
Bondar
Kevin D
KD
Belfield
D000818:Animals; D002470:Cell Survival; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D008247:Lysosomes / Q000378:metabolism*; D008823:Micelles; D057054:Molecular Imaging / Q000379:methods*; D020442:Poloxamer
D005456:Fluorescent Dyes; D008823:Micelles; D020442:Poloxamer
Cancer Commun. 1991 Jul;3(7):207-12
1867954
Science. 1990 Apr 6;248(4951):73-6
2321027
J Biomed Opt. 2005 Sep-Oct;10(5):051402
16292939
Oncogene. 2008 Dec;27 Suppl 1:S137-48
19641499
J Phys Chem B. 2009 May 21;113(20):7101-6
19388691
Science. 2006 Sep 15;313(5793):1642-5
16902090
J Pharm Pharm Sci. 2006;9(3):339-58
17207417
Bioconjug Chem. 2008 Dec;19(12):2559-67
19090700
Nature. 2007 Apr 12;446(7137):745-7
17429391
J Control Release. 2008 Sep 10;130(2):98-106
18534704
Bioconjug Chem. 2008 Oct;19(10):2023-9
18729494
J Neurochem. 2002 Nov;83(3):481-9
12390510
Protein Eng. 1989 Oct;3(1):39-42
2682609
ACS Appl Mater Interfaces. 2009 Oct;1(10):2219-29
20355856
Appl Opt. 1998 Nov 1;37(31):7352-6
18301569
Opt Express. 2008 Mar 17;16(6):4029-47
18542501
Opt Lett. 1994 Jun 1;19(11):780-2
19844443
Brain Pathol. 1998 Jan;8(1):175-93
9458175
Bioconjug Chem. 2009 Oct 21;20(10):1992-2000
19799436
Chem Commun (Camb). 2009 Feb 21;(7):827-9
19322455
J Am Chem Soc. 2010 Sep 8;132(35):12237-9
20712313
J Fluoresc. 2005 Jan;15(1):3-11
15711871
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30
19202073
J Pharmacol Exp Ther. 2001 Nov;299(2):483-93
11602658
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11440-5
16864773
J Org Chem. 2010 Jun 18;75(12):3975-82
20481596