Impacts of Shewanella oneidensis c-type cytochromes on aerobic and anaerobic respiration.
Shewanella are renowned for their ability to utilize a wide range of electron acceptors (EA) for respiration, which has been partially accredited to the presence of a large number of the c-type cytochromes. To investigate the involvement of c-type cytochrome proteins in aerobic and anaerobic respiration of Shewanella oneidensis Mr -1, 36 in-frame deletion mutants, among possible 41 predicted, c-type cytochrome genes were obtained. The potential involvement of each individual c-type cytochrome in the reduction of a variety of EAs was assessed individually as well as in competition experiments. While results on the well-studied c-type cytochromes CymA(SO4591) and MtrC(SO1778) were consistent with previous findings, collective observations were very interesting: the responses of S. oneidensis Mr -1 to low and highly toxic metals appeared to be significantly different; CcoO, CcoP and PetC, proteins involved in aerobic respiration in various organisms, played critical roles in both aerobic and anaerobic respiration with highly toxic metals as EA. In addition, these studies also suggested that an uncharacterized c-type cytochrome (SO4047) may be important to both aerobiosis and anaerobiosis.
College of Life Sciences and Institute of Microbiology, Zhejiang University, Hangzhou, Zhejiang, 310058, China. haichung@zju.edu.cn
Haichun
H
Gao
Soumitra
S
Barua
Yili
Y
Liang
Lin
L
Wu
Yangyang
Y
Dong
Samantha
S
Reed
Jingrong
J
Chen
Dave
D
Culley
David
D
Kennedy
Yunfeng
Y
Yang
Zhili
Z
He
Kenneth H
KH
Nealson
James K
JK
Fredrickson
James M
JM
Tiedje
Margaret
M
Romine
Jizhong
J
Zhou
D000332:Aerobiosis; D000693:Anaerobiosis; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D008670:Metals / Q000378:metabolism* / Q000633:toxicity; D010084:Oxidation-Reduction; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
D008670:Metals; D045304:Cytochromes c
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