Genetic and chemical diversity of high mucilaginous plants of Sida complex by ISSR markers and chemical fingerprinting.
A method was developed based on multiple approaches wherein DNA and chemical analysis was carried out toward differentiation of important species of Sida complex that is being used for commercial preparation. Isolated DNA samples were successfully performed through PCR amplification using ISSR markers and degree of genetic diversity among the different species of Sida is compared with that of chemical diversity. For genetic fingerprint investigation, selected 10 ISSR primers generating reproducible banding patterns were used. Among the total of 63 amplicons, 62 were recorded as polymorphic, genetic similarity index deduced from ISSR profiles ranged from 12 to 51%. Based on similarity index, S. acuta and S. rhombifolia found to be most similar (51%). High number of species-specific bands played pivotal role to delineate species at genetic level. Investigation based on HPTLC fingerprints analysis revealed 23 bands representing to characteristic chemicals and similarity index ranged from 73 to 91%. Prominent distinguishable bands were observed only in S. acuta, while S. cordifolia and S. rhombifolia shared most bands making them difficult to identify on chemical fingerprint basis. This report summarizes the genotypic and chemotypic diversity and the use of profiles for authentication of species of Sida complex.
Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CIMAP-CSIR), Lucknow, 226016, UP, India. sanjay.thul@gmail.com
Sanjog T
ST
Thul
Ankit K
AK
Srivastava
Subhash C
SC
Singh
Karuna
K
Shanker
D002851:Chromatography, High Pressure Liquid; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D019660:Malvaceae / Q000737:chemistry* / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269-73
291943
J Ethnopharmacol. 2003 Dec;89(2-3):291-4
14611894
Indian J Pharm Sci. 2009 May;71(3):323-4
20490305
Nat Prod Res. 2009;23(8):689-95
19418351
Mol Biotechnol. 2005 Oct;31(2):129-35
16170213
Planta Med. 1980 May;39(1):66-72
7403308
Planta Med. 2009 Sep;75(11):1266-70
19350479
Planta Med. 1981 Dec;43(4):384-8
17402065
Phytother Res. 2003 Sep;17(8):973-5
13680839
Biotechniques. 1992 Jul;13(1):52-4, 56
1503775