GABAergic and glutamatergic identities of developing midbrain Pitx2 neurons.
Pitx2, a paired-like homeodomain transcription factor, is expressed in post-mitotic neurons within highly restricted domains of the embryonic mouse brain. Previous reports identified critical roles for PITX2 in histogenesis of the hypothalamus and midbrain, but the cellular identities of PITX2-positive neurons in these regions were not fully explored. This study characterizes Pitx2 expression with respect to midbrain transcription factor and neurotransmitter phenotypes in mid-to-late mouse gestation. In the dorsal midbrain, we identified Pitx2-positive neurons in the stratum griseum intermedium (SGI) as GABAergic and observed a requirement for PITX2 in GABAergic differentiation. We also identified two Pitx2-positive neuronal populations in the ventral midbrain, the red nucleus, and a ventromedial population, both of which contain glutamatergic precursors. Our data suggest that PITX2 is present in regionally restricted subpopulations of midbrain neurons and may have unique functions that promote GABAergic and glutamatergic differentiation.
Cellular and Molecular Biology Program, The University of Michigan, Ann Arbor, Michigan, USA.
M R
MR
Waite
J M
JM
Skidmore
A C
AC
Billi
J F
JF
Martin
D M
DM
Martin
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D008636:Mesencephalon / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*; D000097577:Homeobox Protein PITX2
D018698:Glutamic Acid; D018398:Homeodomain Proteins; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid; D000097577:Homeobox Protein PITX2
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