Proteome of human subcutaneous adipose tissue stromal vascular fraction cells versus mature adipocytes based on DIGE.
Adipose tissue contains a heterogeneous population of mature adipocytes, endothelial cells, immune cells, pericytes, and preadipocytic stromal/stem cells. To date, a majority of proteomic analyses have focused on intact adipose tissue or isolated adipose stromal/stem cells in vitro. In this study, human subcutaneous adipose tissue from multiple depots (arm and abdomen) obtained from female donors was separated into populations of stromal vascular fraction cells and mature adipocytes. Out of 960 features detected by 2-D gel electrophoresis, a total of 200 features displayed a 2-fold up- or down-regulation relative to each cell population. The protein identity of 136 features was determined. Immunoblot analyses comparing SVF relative to adipocytes confirmed that carbonic anhydrase II was up-regulated in both adipose depots while catalase was up-regulated in the arm only. Bioinformatic analyses of the data set determined that cytoskeletal, glycogenic, glycolytic, lipid metabolic, and oxidative stress related pathways were highly represented as differentially regulated between the mature adipocytes and stromal vascular fraction cells. These findings extend previous reports in the literature with respect to the adipose tissue proteome and the consequences of adipogenesis. The proteins identified may have value as biomarkers for monitoring the physiology and pathology of cell populations within subcutaneous adipose depots.
Proteomics and Metabolomics Core Facility, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, Louisiana 70808, United States.
Indu
I
Kheterpal
Ginger
G
Ku
Liana
L
Coleman
Gang
G
Yu
Andrey A
AA
Ptitsyn
Z Elizabeth
ZE
Floyd
Jeffrey M
JM
Gimble
D017667:Adipocytes / Q000737:chemistry*; D000328:Adult; D024402:Carbonic Anhydrase II / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D019295:Computational Biology / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D042783:Endothelial Cells / Q000737:chemistry* / Q000166:cytology; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D010641:Phenotype; D020543:Proteome / Q000032:analysis*; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology; D017154:Stromal Cells / Q000737:chemistry* / Q000166:cytology; D050151:Subcutaneous Fat / Q000166:cytology*
D020543:Proteome; D024402:Carbonic Anhydrase II
Stem Cells. 2006 May;24(5):1246-53
16410391
Cell Tissue Res. 1977 Jul 11;181(2):197-203
195732
Am J Physiol. 1993 Jul;265(1 Pt 1):C234-43
8338133
Exp Cell Res. 2003 Jan 1;282(1):14-23
12490190
Proteomics. 2004 Feb;4(2):438-41
14760714
J Clin Endocrinol Metab. 2002 Feb;87(2):728-34
11836312
Methods Mol Biol. 2008;449:69-79
18370084
Ann Plast Surg. 2008 May;60(5):538-44
18434829
J Biol Chem. 2000 Mar 31;275(13):9441-6
10734090
Biochem Biophys Res Commun. 2008 Feb 15;366(3):698-704
18073138
Nat Med. 2009 Aug;15(8):930-9
19633656
Int J Obes (Lond). 2008 Dec;32 Suppl 7:S39-51
19136990
J Proteome Res. 2009 Apr;8(4):1682-93
19714809
Physiol Genomics. 2009 Oct 7;39(2):109-19
19602617
Mol Cell Biol. 1999 Jan;19(1):495-504
9858573
Nature. 1970 Aug 15;227(5259):680-5
5432063
Cancer Res. 1992 Jul 1;52(13):3814-7
1617653
Metabolism. 2008 Jul;57(7):1005-15
18555844
Proteomics. 2010 Sep;10(18):3356-66
20706982
Dev Cell. 2002 Mar;2(3):331-41
11879638
Biochem Biophys Res Commun. 2009 Jul 10;384(4):482-5
19422792
J Biol Chem. 1964 Feb;239:375-80
14169133
Mol Cell Biol. 1996 Aug;16(8):4273-80
8754828
Mol Cell Proteomics. 2005 Apr;4(4):492-522
15695426
Diabetes. 2008 Sep;57(9):2438-44
18567819
J Clin Invest. 1989 Nov;84(5):1663-70
2681273
J Cell Physiol. 2008 Feb;214(2):413-21
17654479
Proc Nutr Soc. 2001 Aug;60(3):329-39
11681807
Nat Med. 2009 Aug;15(8):914-20
19633658
Obesity (Silver Spring). 2010 Jan;18(1):27-34
19556978
J Proteome Res. 2010 May 7;9(5):2438-49
20199104
J Proteome Res. 2010 Oct 1;9(10):5389-401
20681635
Proteomics. 2010 Feb;10(3):394-405
19953544
J Clin Invest. 2003 Dec;112(12):1821-30
14679177
J Lipid Res. 1978 Mar;19(3):316-24
206638
Stem Cells. 2006 Feb;24(2):376-85
16322640
Proteomics. 2009 Jun;9(11):2955-66
19415655
Phytother Res. 2010 Sep;24(9):1278-84
20175108
J Biol Chem. 1966 Jan 10;241(1):130-9
4379054
J Biol Chem. 1966 Jan 10;241(1):140-2
4285132
Mol Cell Proteomics. 2005 Jun;4(6):731-40
15753122
Methods Mol Biol. 2011;702:163-90
21082402
J Biol Chem. 1966 Sep 10;241(17):3909-17
4288359
FASEB J. 1996 Mar;10(4):481-90
8647347
Eur J Hum Genet. 2001 Mar;9(3):204-8
11313760
Genes Dev. 1992 Mar;6(3):439-53
1547942
Mol Cell Biol. 1992 Mar;12(3):1202-8
1545801
J Proteome Res. 2010 Sep 3;9(9):4521-34
20812759
J Immunol. 2010 Aug 1;185(3):1836-45
20581149
J Cell Biochem. 2007 Aug 15;101(6):1545-57
17370314
Mol Cell Biol. 1989 Oct;9(10):4196-203
2573827
Cytotherapy. 2010 Jul;12(4):538-46
20380539
Acta Physiol Scand. 2005 Aug;184(4):285-93
16026420
Mol Cell Proteomics. 2007 Jan;6(1):18-28
17018519
Circ Res. 2008 Jan 4;102(1):77-85
17967785
Nat Med. 2009 Aug;15(8):921-9
19633657
Proteomics. 2008 May;8(9):1744-9
18442176
Methods Mol Biol. 2008;456:155-71
18516560
Mol Cell Proteomics. 2008 Aug;7(8):1570-83
18474516
J Clin Invest. 2005 May;115(5):1111-9
15864338
Curr Proteomics. 2010 Apr 1;7(1):49-65
20526421
Arterioscler Thromb Vasc Biol. 2009 Oct;29(10):1608-14
19644053
Int J Obes. 1987;11(1):19-27
3570635
Mol Cell Proteomics. 2009 Feb;8(2):316-24
18840871
J Cell Biochem. 2007 Feb 15;100(3):762-72
17031858
J Cell Physiol. 2005 Oct;205(1):114-22
15880450
J Clin Invest. 2003 Dec;112(12):1796-808
14679176
Mol Cell Proteomics. 2007 Apr;6(4):589-600
17255083