Effect of trichostatin A on fertilization and embryo development during extended culture of mouse oocyte.
We performed this study to investigate the effect of histone deacetylase inhibition during extended culture of in vitro matured mouse oocytes. In vitro matured mouse (BDF1) oocytes were cultured in vitro for 6, 12, and 24 h, respectively, and then inseminated. During in vitro culture for 6 and 12 h, two doses of trichostatin A (TSA), a histone deacetylase inhibitor, were added (100 nM and 500 nM) to the culture medium and the oocytes were then inseminated. During the 24-h in vitro culture, two doses of TSA were added (100 nM and 500 nM) to the medium and the oocytes were activated with 10 mM SrCl2. After the 6-h culture, the fertilization rate was similar to that of the control group, but the blastocyst formation rate was significantly decreased. After the 12-h culture, both the fertilization and blastocyst formation rates were significantly decreased. After the 24-h culture, total fertilization failure occurred. In the oocytes cultured for 6 and 12 h, the fertilization and blastocyst formation rates did not differ between the TSA-supplemented and control groups. Although extended culture of the mouse oocytes significantly affected their fertilization and embryo development, TSA supplementation did not overcome their decreased developmental potential.
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, 463-707, Korea.
Byung Chul
BC
Jee
Jun Woo
JW
Jo
Jung Ryeol
JR
Lee
Chang Suk
CS
Suh
Seok Hyun
SH
Kim
Shin Yong
SY
Moon
D000818:Animals; D016922:Cellular Senescence; D003470:Culture Media / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology*; D059471:In Vitro Oocyte Maturation Techniques; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D010312:Parthenogenesis; D013997:Time Factors
D003470:Culture Media; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A