[Study on the purification and scavenging free radical activity of water soluble polysaccharide from Ligularia hodgsonii].
OBJECTIVE
The study was to provided the reference for further development and utilization of Ligularia hodgsonii.


METHODS
The crude polysaccharide LW was extracted from Lugularia hodgsonii with hot water, and prectated by ethanol. The crude polysaccharide has been fractionted and purified, so LW21 was got. Its homogeneity was confirmed by chromatography on Sepharose CL-4B, paper chromatography and cellulous actate electrophoresis. The scavenging effets of the polysacehardes on * OH and O2-* were determined by fluorescence method.


RESULTS
Antioxidative experiment shows that LW and LW21 were effective in scavenging superoxide radical and hydroxyl radical. The scavenging capacity for superoxide radical and hydroxyl radical of LW21 had higher than that of LW.


CONCLUSIONS
The polysaceharide from Ligularia hodgsonii had stronger antioxidant actioxdant activities, and their antioxidant activities had certain dose-relationship.
College of Life and Environmental Science, MINZU University of China, Beijing 100081, China. liuchunlan888@yahoo.com.cn
Chun-Lan
CL
Liu
Yang
Y
Li
Ning
N
Du
Xiao
X
Huang
Wen-Shu
WS
Wang
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D000431:Ethanol; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D014867:Water
D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides; D014867:Water; D017665:Hydroxyl Radical; D000431:Ethanol