A reliable method for storage of tailed phages.
A universal and effective method for long-term storage of bacteriophages has not yet been described. We show that randomly selected tailed phages could be stored inside the infected cells at -80°C without a major loss of phage and host viability. Our results suggest the suitability of this method as a standard for phage preservation.
Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland. pgolec@biotech.ug.gda.pl
Piotr
P
Golec
Kamil
K
Dąbrowski
Monika S
MS
Hejnowicz
Agnieszka
A
Gozdek
Joanna M
JM
Loś
Grzegorz
G
Węgrzyn
Małgorzata B
MB
Lobocka
Marcin
M
Loś
D001419:Bacteria / Q000821:virology; D001435:Bacteriophages / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*