[Effect of prolonged infusion of propofol on the liver mitochondria respiratory function in rabbits].
OBJECTIVE
To investigate the changes of respiratory function of liver mitochondria in rabbits induced by the general anesthetic propofol.


METHODS
Eighteen New Zealand rabbits weighted 1.5-2.5 kg were randomly divided into three groups: control group, emulsion group and propofol group. The control group received continuous infusion of 0.9% sodium chloride solution. The propofol group received continuous infusion of 1% propofol. The emulsion group received continuous infusion of 10% emulsion. The liver mitochondri of the rabbits were isolated. The carnitine acyl transferase (CPT) activity, H+ -ATPase hydrolysis activity and the content of ATP in the mitochondria were analysed.


RESULTS
The rabbits in the propofol group had lower activity of CPT than the controls (P < 0.05), while no difference was found between the control group and the emulsion group (P > 0.05). The rabbits in the propofol group had higher H+ -ATPase hydrolysis activity than the controls (P < 0.05), while no difference was found between the control group and the emulsion group (P > 0.05). No significant differences were found in the content of ATP in mitochondria between the three groups (P > 0.05).


CONCLUSIONS
Propofol inhibits CPT activity, which disturbs fatty acid beta-oxidation. Emulsion acted as vehicle of propofol seems to have no significant impact on mitochondria respiratory function.
Laboratory of Anesthesia and Cirtical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Xian-xiang
XX
Tong
Yi
Y
Kang
Feng-zhi
FZ
Liu
Wen-sheng
WS
Zhang
Jin
J
Liu
D000255:Adenosine Triphosphate / Q000032:analysis; D018686:Anesthetics, Intravenous / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D002333:Carnitine Acyltransferases / Q000378:metabolism; D019069:Cell Respiration / Q000187:drug effects; D005260:Female; D007262:Infusions, Intravenous; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D015742:Propofol / Q000008:administration & dosage / Q000633:toxicity*; D011817:Rabbits
D018686:Anesthetics, Intravenous; D000255:Adenosine Triphosphate; D002333:Carnitine Acyltransferases; D025261:Mitochondrial Proton-Translocating ATPases; D015742:Propofol