Longer-range distances by spinning-angle-encoding solid-state NMR spectroscopy.
A new spinning-angle-encoding spin-echo solid-state NMR approach is used to accurately determine the dipolar coupling corresponding to a C-C distance over 4 Å in a fully labelled dipeptide. The dipolar coupling dependent spin-echo modulation was recorded off magic angle, switching back to the magic angle for the acquisition of the free-induction decay, so as to obtain optimum sensitivity. The retention of both ideal resolution and long-range distance sensitivity was achieved by redesigning a 600 MHz HX MAS NMR probe to provide fast angle switching during the NMR experiment: for 1.8 mm rotors, angle changes of up to ∼5° in ∼10 ms were achieved at 12 kHz MAS. A new experimental design that combines a reference and a dipolar-modulated experiment and a master-curve approach to data interpretation is presented.
Department of Physics, University of Warwick, Coventry, UK CV4 7AL.
Johanna
J
Becker-Baldus
Thomas F
TF
Kemp
Jaan
J
Past
Andres
A
Reinhold
Ago
A
Samoson
Steven P
SP
Brown
D004151:Dipeptides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D004151:Dipeptides