Increased memory conversion of naïve CD8 T cells activated during late phases of acute virus infection due to decreased cumulative antigen exposure.
BACKGROUND
Memory CD8 T cells form an essential part of protective immunity against viral infections. Antigenic load, costimulation, CD4-help, cytokines and chemokines fluctuate during the course of an antiviral immune response thus affecting CD8 T cell activation and memory conversion.


RESULTS
In the present study, naïve TCR transgenic LCMV-specific P14 CD8 T cells engaged at a late stage during the acute antiviral LCMV response showed reduced expansion kinetics but greater memory conversion in the spleen. Such late activated cells displayed a memory precursor effector phenotype already at the peak of the systemic antiviral response, suggesting that the environment determined their fate during antigen encounter. In the spleen, the majority of late transferred cells exhibited a central memory phenotype compared to the effector memory displayed by the early transferred cells. Increasing the inflammatory response by exogenous administration of IFNγ, PolyI:C or CpG did not affect memory conversion in the late transferred group, suggesting that the diverging antigen load early versus later during acute infection had determined their fate. In agreement, reduction in the LCMV antigenic load after ribavirin treatment enhanced the contribution of early transferred cells to the long lasting memory pool.


CONCLUSIONS
Our results show that naïve CD8 cells, exposed to reduced duration or concentration of antigen during viral infection convert into memory more efficiently, an observation that could have significant implications for vaccine design.
Diabetes Center, La Jolla Institute for Allergy and Immunology, La Jolla, California, United States of America.
Georgia
G
Fousteri
Amy
A
Dave
Amy
A
Juedes
Therese
T
Juntti
Bret
B
Morin
Lisa
L
Togher
Donna L
DL
Farber
Matthias
M
von Herrath
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D007109:Immunity; D007156:Immunologic Memory; D007249:Inflammation; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology; D013997:Time Factors; D014777:Virus Diseases / Q000276:immunology*
D000956:Antigens, Viral
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