Capillary electrophoretic separation of nanoparticles.
In the present work, CdSe nanocrystals (NCs) synthesized with a trioctylphosphine surface passivation layer were modified using amphiphilic molecules to form a surface bilayer capable of providing stable NCs aqueous solutions. Such modified nanocrystals were used as a test solute in order to analyze new electrophoretic phenomena, by applying a micellar plug as a separation tool for discriminating nanocrystals between micellar and micelle-free zones during electrophoresis. The distribution of NCs between both zones depended on the affinity of nanocrystals towards the micellar zone, and this relies on the kind of surface ligands attached to the NCs, as well as electrophoretic conditions applied. In this case, the NCs that migrated within a micellar zone can be focused using a preconcentration mechanism. By modifying electrophoretic conditions, NCs were forced to migrate outside the micellar zone in the form of a typical CZE peak. In this situation, a two-order difference in separation efficiencies, in terms of theoretical plates, was observed between focused NCs (N ~ 10(7)) and a typical CZE peak for NCs (N ~ 10(5)). By applying the amino-functionalized NCs the preconcentration of NCs, using a micellar plug, was examined, with the conclusion that preconcentration efficiency, in terms of the enhancement factor for peak height (SEF(height)) can be, at least 20. The distribution effect was applied to separate CdSe/ZnS NCs encapsulated in silica, as well as surface-modified with DNA, which allows the estimation of the yield of conjugation of biologically active molecules to a particle surface.
Faculty of Chemistry, Department of Analytical Chemistry, Warsaw University of Technology, Poland. slaosw@ch.pw.edu.pl
Sławomir
S
Oszwałdowski
Katarzyna
K
Zawistowska-Gibuła
Kenneth P
KP
Roberts
D000327:Adsorption; D001681:Biological Assay / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*
D004247:DNA
Science. 2005 Jan 28;307(5709):538-44
15681376
J Chromatogr A. 2008 Dec 19;1214(1-2):171-7
18990396
Langmuir. 2010 Jan 19;26(2):736-42
19950951
J Chromatogr A. 2009 Dec 25;1216(52):9034-47
19631942
J Am Chem Soc. 2006 May 17;128(19):6324-5
16683790
J Chromatogr A. 2007 Jul 13;1156(1-2):75-9
17208246
Anal Bioanal Chem. 2009 Nov;395(6):1681-91
19578837
J Chromatogr A. 2007 Aug 17;1161(1-2):314-21
17559859
Science. 2002 Nov 29;298(5599):1759-62
12459582
Talanta. 2006 Sep 15;70(2):397-402
18970782
Anal Bioanal Chem. 2010 Jan;396(1):229-40
19714321
Electrophoresis. 2009 May;30(10):1670-4
19378283
Electrophoresis. 2008 Feb;29(3):576-89
18228534
Electrophoresis. 2009 Mar;30(5):875-82
19260005
Anal Chem. 2006 Aug 15;78(16):5925-32
16906742
J Am Chem Soc. 2006 Jul 26;128(29):9288-9
16848437
J Chromatogr A. 2006 Apr 28;1113(1-2):251-4
16563405
J Chromatogr A. 2000 Mar 24;873(2):257-67
10757303
Anal Chem. 2008 Mar 15;80(6):1988-94
18278946
Anal Chem. 2008 Sep 1;80(17):6824-9
18671411
Chem Rev. 2000 Jul 12;100(7):2505-36
11749294
Nano Lett. 2005 Apr;5(4):645-8
15826102
Electrophoresis. 2002 Jul;23(13):1957-72
12210247
J Phys Chem B. 2006 Oct 19;110(41):20308-16
17034212
J Chromatogr A. 2007 Oct 12;1167(2):231-5
17850805
J Chromatogr A. 2009 Mar 20;1216(12):2554-9
19167005
Electrophoresis. 2007 Aug;28(16):2874-81
17647216
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Feb 5;800(1-2):7-25
14698231
J Am Chem Soc. 2002 Mar 27;124(12):3169-74
11902906
Anal Chem. 2008 Feb 15;80(4):1255-62
18201071
Annu Rev Phys Chem. 2007;58:635-73
17163837
Chem Soc Rev. 2007 Apr;36(4):579-91
17387407
Anal Bioanal Chem. 2009 May;394(1):175-85
19255749
J Colloid Interface Sci. 2007 May 15;309(2):194-224
17368660
J Sep Sci. 2009 Jun;32(11):1889-906
19479769
Anal Chem. 2002 Nov 1;74(21):5523-30
12433083
Nat Mater. 2005 Jun;4(6):435-46
15928695