[Study on extraction of shikimic acid from pine needles of Pinus elliottii by decompressing inner ebullition].
We studied the extracting process of shikimic acid from pine needles of Pinus elliottii Engelm, using pine needles of Pinus elliottii Engelm as raw materials, a high concentration of alcohol as the desorption and a low concentration of alcohol as the hot-extraction, and the decompressing inner ebullition method. Through the single factor test and orthogonal test, the best processing of the decompressing inner ebullition was as follows: when the concentration of alcohol was 70 percent with 18 minutes' desorption, the temperature of hot-extraction was 65 degrees C with 4 minutes' hot-extraction, solid-liquid ratio was 1:1.6 with 260 mL of hot-extraction, with a concentration of 30%. The extracting effect was the best when extracted twice. The extracting proportion of shikimic acid from dry pine needles of Pinus elliottii was 1.51%.
School of Chemistry & Chemical Engineering, Guangxi University, Nanning 530004, China. lzszxjy@126.com
Ji-Yun
JY
Xie
Xiao-Peng
XP
Chen
Fang
F
Chen
D002851:Chromatography, High Pressure Liquid; D003664:Decompression; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D028223:Pinus; D018515:Plant Leaves / Q000737:chemistry; D012765:Shikimic Acid / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D012997:Solvents; D012765:Shikimic Acid; D000431:Ethanol