LC-MS/MS-ESI method for simultaneous quantitation of three endocannabinoids and its application to rat pharmacokinetic studies.
An LC-MS/MS-ESI method has been validated for simultaneous estimation of the three endocannabinoids; N-arachidonoylethanolamine (AEA), N-oleoylethanolamine (OEA) and palmitoylethanolamide (PEA), in surrogate matrix using AEA-d (4) as an internal standard with highest sensitivity over the existing methods. Simple precipitation was used to extract analytes and these were subsequently analyzed on a monolithic column. Linear response function was established over the concentration range 12.3 to 1225 pg/ml for AEA (r > 0.994); 0.70 to 641 ng/ml for OEA (r > 0.999) and 0.54 to 321 ng/ml (r > 0.998) for PEA. The intra- and inter-day precision values met the acceptance to criteria as per US FDA guidelines. Analytes were found to be stable in the battery of stability studies. The method was applied to quantify endogenous levels of analytes in rat plasma.
Jubilant Biosys, Industrial Suburb, 2nd Stage, Yeshwanthpur, Bangalore-560 022, India.
Kuldeep
K
Sharma
Radhe Raman
RR
Singh
Murugesh
M
Kandaswamy
Chandan
C
Mithra
Sanjeev
S
Giri
Sriram
S
Rajagopal
Ramesh
R
Mullangi
D000577:Amides; D000818:Animals; D001095:Arachidonic Acids / Q000097:blood; D063385:Cannabinoid Receptor Modulators / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D063388:Endocannabinoids; D004983:Ethanolamines / Q000097:blood; D009829:Oleic Acids; D010169:Palmitic Acids / Q000097:blood; D053284:Polyunsaturated Alkamides; D051381:Rats; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000577:Amides; D001095:Arachidonic Acids; D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D004983:Ethanolamines; D009829:Oleic Acids; D010169:Palmitic Acids; D053284:Polyunsaturated Alkamides; C033595:N-oleoylethanolamine; C005958:palmidrol; C078814:anandamide