A novel 3-hydroxyproline (3Hyp)-rich motif marks the triple-helical C terminus of tendon type I collagen.
Because of its unique physical and chemical properties, rat tail tendon collagen has long been favored for crystallographic and biochemical studies of fibril structure. In studies of the distribution of 3-hydroxyproline in type I collagen of rat bone, skin, and tail tendon by mass spectrometry, the repeating sequences of Gly-Pro-Pro (GPP) triplets at the C terminus of α1(I) and α2(I) chains were shown to be heavily 3-hydroxylated in tendon but not in skin and bone. By isolating the tryptic peptides and subjecting them to Edman sequence analysis, the presence of repeating 3-hydroxyprolines in consecutive GPP triplets adjacent to 4-hydroxyproline was confirmed as a unique feature of the tendon collagen. A 1960s study by Piez et al. (Piez, K. A., Eigner, E. A., and Lewis, M. S. (1963) Biochemistry 2, 58-66) in which they compared the amino acid compositions of rat skin and tail tendon type I collagen chains indeed showed 3-4 residues of 3Hyp in tendon α1(I) and α2(I) chains but only one 3Hyp residue in skin α1(I) and none in α2(I). The present work therefore confirms this difference and localizes the additional 3Hyp to the GPP repeat at the C terminus of the triple-helix. We speculate on the significance in terms of a potential function in contributing to the unique assembly mechanism and molecular packing in tendon collagen fibrils and on mechanisms that could regulate 3-hydroxylation at this novel substrate site in a tissue-specific manner.
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195-6500. Electronic address: deyre@u.washington.edu.
David R
DR
Eyre
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195-6500.
MaryAnn
M
Weis
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195-6500.
David M
DM
Hudson
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195-6500.
Jiann-Jiu
JJ
Wu
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195-6500.
Lammy
L
Kim
D020816:Amino Acid Motifs; D000818:Animals; D024042:Collagen Type I / Q000737:chemistry* / Q000378:metabolism; D006900:Hydroxylation / Q000502:physiology; D006909:Hydroxyproline / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D020836:Protein Structure, Quaternary; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D013710:Tendons / Q000737:chemistry* / Q000378:metabolism
D024042:Collagen Type I; D006909:Hydroxyproline
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