Transgenic expression of glucose dehydrogenase in Azotobacter vinelandii enhances mineral phosphate solubilization and growth of sorghum seedlings.
The enzyme quinoprotein glucose dehydrogenase (GDH) catalyses the oxidation of glucose to gluconic acid by direct oxidation in the periplasmic space of several Gram-negative bacteria. Acidification of the external environment with the release of gluconic acid contributes to the solubilization of the inorganic phosphate by biofertilizer strains of the phosphate-solubilizing bacteria. Glucose dehydrogenase (gcd) gene from Escherichia coli, and Azotobacter-specific glutamine synthetase (glnA) and phosphate transport system (pts) promoters were isolated using sequence-specific primers in a PCR-based approach. Escherichia coli gcd, cloned under the control of glnA and pts promoters, was mobilized into Azotobacter vinelandii AvOP and expressed. Sorghum seeds were bacterized with the transgenic azotobacters and raised in earthen pots in green house. The transgenic azotobacters, expressing E. coli gcd, showed improved biofertilizer potential in terms of mineral phosphate solubilization and plant growth-promoting activity with a small reduction in nitrogen fixation ability.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad-500 046, Andhra Pradesh, India.
Burla
B
Sashidhar
Appa Rao
AR
Podile
D016948:Azotobacter vinelandii / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D042843:Glucose 1-Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D007287:Inorganic Chemicals / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology; D045868:Sorghum / Q000254:growth & development* / Q000382:microbiology
D007287:Inorganic Chemicals; D010710:Phosphates; D011994:Recombinant Proteins; D042843:Glucose 1-Dehydrogenase
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