[Study on the enrichment and purification of total flavonoids in Schizonepeta tenuifolia by macroporous adsorption resin].
OBJECTIVE
To establish an optimum enrichment and purification process of total flavonoids in Schizonepeta tenuifolia by macroporous resins.


METHODS
9 kinds of resins were compared and the best one was chosen. Then the technique condition for separating and purifying total flavonoids from Schizonepeta tenuifolia was studied.


RESULTS
HP-20 was selected for its excellent adsorption and desorption properties, 80% ethanol was found to be the best elution solution. The best result was based on the follows: pH value-6.0, feeding rate-2 BV/h,sample concentration-0.4 g/mL, the ratio of total flavonoids to HP-20 macroporous resin-133. 76 mg/g, elution flowing rate-4 BV/h, eluting agent amount-3 BV. Then the purity reached 74. 31% and the yield was 97.13%.


CONCLUSIONS
The total flavonoids of Schizonepeta tenuifolia can be effectively purificated and separated by HP-20 macroporous resin.
Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China. hzh193@yahoo.com.cn
Zhi-Hong
ZH
Huang
Dong-Xu
DX
Jiang
Xiao-Ping
XP
Lai
D000327:Adsorption; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D016062:Porosity; D012117:Resins, Synthetic; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*
D005419:Flavonoids; D012117:Resins, Synthetic