Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats.
The protective effect of coenzyme Q10 against testicular toxicity induced by sodium arsenite (10mg/kg/day, orally for two consecutive days) was investigated in rats. Coenzyme Q10 treatment (10mg/kg/day, i.p.) was applied for five consecutive days, starting three days before arsenite administration. Coenzyme Q10 significantly increased serum testosterone level which was reduced by sodium arsenite. Coenzyme Q10 significantly suppressed lipid peroxidation, restored the depleted antioxidant defenses, and attenuated the increases of tumor necrosis factor-α and nitric oxide resulted from arsenic administration. Also, the elevation of arsenic ion, and the reductions of selenium and zinc ions in testicular tissue were mitigated by coenzyme Q10. Histopathological examination showed that testicular injury mediated by arsenic was ameliorated by coenzyme Q10 treatment. Immunohistochemical analysis revealed that coenzyme Q10 significantly decreased the arsenic-induced expression of inducible nitric oxide synthase, nuclear factor-κB, Fas ligand and caspase-3 in testicular tissue. It was concluded that coenzyme Q10 represents a potential therapeutic option to protect the testicular tissue from the detrimental effects of arsenic intoxication.
Department of Biomedical Sciences, Pharmacology Division, College of Medicine, Al-Ahsa, King Faisal University, Saudi Arabia. amrfouad65@yahoo.com
Amr A
AA
Fouad
Ali Ibrahim
AI
Al-Sultan
Mohamed T
MT
Yacoubi
D000818:Animals; D001151:Arsenic / Q000378:metabolism; D018053:Arsenites / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000378:metabolism; D017670:Sodium Compounds / Q000633:toxicity*; D013737:Testis / Q000187:drug effects* / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism
D018053:Arsenites; D017670:Sodium Compounds; D014451:Ubiquinone; D013739:Testosterone; C017947:sodium arsenite; C024989:coenzyme Q10; D012643:Selenium; D015032:Zinc; D001151:Arsenic