Oxidative damage in lymphocytes of copper smelter workers correlated to higher levels of excreted arsenic.
Arsenic has been associated with multiple harmful effects at the cellular level. Indirectly these defects could be related to impairment of the integrity of the immune system, in particular in lymphoid population. To characterize the effect of Arsenic on redox status on this population, copper smelter workers and arsenic unexposed donors were recruited for this study. We analyzed urine samples and lymphocyte enriched fractions from donors to determinate arsenic levels and lymphocyte proliferation. Moreover, we studied the presence of oxidative markers MDA, vitamin E and SOD activity in donor plasma. Here we demonstrated that in human beings exposed to high arsenic concentrations, lymphocyte MDA and arsenic urinary levels showed a positive correlation with SOD activity, and a negative correlation with vitamin E serum levels. Strikingly, lymphocytes from the arsenic exposed population respond to a polyclonal stimulator, phytohemaglutinin, with higher rates of thymidine incorporation than lymphocytes of a control population. As well, similar in vitro responses to arsenic were observed using a T cell line. Our results suggest that chronic human exposure to arsenic induces oxidative damage in lymphocytes and could be considered more relevant than evaluation of T cell surveillance.
Instituto de Química, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso (PUCV), Avenida Brasil 119, Casilla 4059 Valparaíso, Chile.
Jorge
J
Escobar
Lorena
L
Varela-Nallar
Claudio
C
Coddou
Pablo
P
Nelson
Kevin
K
Maisey
Daniel
D
Valdés
Alexis
A
Aspee
Victoria
V
Espinosa
Carlos
C
Rozas
Margarita
M
Montoya
Cristian
C
Mandiola
Felipe E
FE
Rodríguez
Claudio
C
Acuña-Castillo
Alejandro
A
Escobar
Ricardo
R
Fernández
Hernán
H
Diaz
Mario
M
Sandoval
Mónica
M
Imarai
Miguel
M
Rios
D000328:Adult; D001151:Arsenic / Q000633:toxicity* / Q000652:urine; D015992:Body Mass Index; D016022:Case-Control Studies; D049109:Cell Proliferation; D003300:Copper / Q000633:toxicity*; D006801:Humans; D007221:Industry; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
D014810:Vitamin E; D003300:Copper; D013482:Superoxide Dismutase; D001151:Arsenic
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