Impact of ultrafiltration membrane material on Peptide separation from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes.
Electrodialysis with ultrafiltration membrane (EDUF) is a technology based on the separation of molecules according to their charge and molecular mass. Some works have already successfully demonstrated the recovery of bioactive peptide fractions. However, the impact of ultrafiltration membrane (UFM) material, used in the EDUF system, on the peptide migration has never been studied. Consequently, the objectives of this work were (1) to evaluate the effect of two different UFM materials on the selective separation of peptides from a snow crab byproduct hydrolysate by electrodialysis with ultrafiltration membranes and (2) to determine the effect of UFM material on their potential fouling by peptides. It appeared that, after 6 h of EDUF separation using polyether sulfone (PES) and cellulose acetate (CA) UFM, peptides with low molecular weights ranging from 300 to 700 Da represented the most abundant population in the KCl1 (compartment located near the anode for the recovery of anionic/acid peptide fractions) and KCl2 (compartment located near the cathode for the recovery of cationic/basic peptide fractions) permeates. Peptides with molecular weights ranging from 700 to 900 Da did not migrate during the EDUF treatment. Moreover, only CA UFM allowed the recovery of high molecular weight molecules (900-20000 Da) in both KCl compartments. Peptides desorbed from PES and CA UFM after 6 h of EDUF separation had low molecular weights and belonged mainly to the 600-700 Da molecular weight range. These peptides represented a low proportion of the peptides initially present in the snow crab byproduct hydrolysate with individual molecular weight range proportions from 1.52 ± 0.31 to 10.2 ± 2.32%.
Institute of Nutraceuticals and Functionnal Foods (INAF).
Alain
A
Doyen
Lucie
L
Beaulieu
Linda
L
Saucier
Yves
Y
Pouliot
Laurent
L
Bazinet
D000818:Animals; D003386:Brachyura / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives; D003956:Dialysis / Q000295:instrumentation / Q000379:methods; D004553:Electric Conductivity; D005612:Freeze Drying; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D011108:Polymers; D011189:Potassium Chloride; D012996:Solutions; D013450:Sulfones; D014462:Ultrafiltration / Q000295:instrumentation*
D010455:Peptides; D011108:Polymers; D012996:Solutions; D013450:Sulfones; C022840:polyether sulfone; C005062:acetylcellulose; D011189:Potassium Chloride; D002482:Cellulose