Fitness costs linked to dinitroaniline resistance mutation in Setaria.
A mutant Thr-239-Ileu at the α2-tubulin gene was found to confer resistance to dinitroanilines, a family of mitosis-disrupting herbicides. However, mutations affecting microtubule polymerization and cell division are expected to impact growth and reproduction, that is, the fitness of a resistant weed or the yield of a tolerant crop, although it has not been demonstrated yet. This study was designed to test this hypothesis for the growth and reproduction of near-isogenic resistant and susceptible materials that were created in F(2) and F(3) generations after a Setaria viridis x S. italica cross. Differential growth was noticeable at the very onset of seedling growth. The homozygous resistant plants, grown both in a greenhouse cabinet and in the field, were smaller and had lower 1000-grain weight and therefore a lower yield. This fitness penalty is certainly due to modified cell division kinetics. Although the presence of the mutant allele accounted for 20% yield losses, there were also measurable benefits of dinitroaniline resistance, and these benefits are discussed.
INRA, UMR 1210 Biologie et Gestion des Adventices, Dijon, France. darmency@dijon.inra.fr
H
H
Darmency
J C
JC
Picard
T
T
Wang
D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D053209:Herbicide Resistance / Q000235:genetics; D006540:Herbicides / Q000494:pharmacology*; D009154:Mutation; D032983:Setaria Plant / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D000814:Aniline Compounds; D006540:Herbicides; C008625:nitroaniline
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