Simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika by HPLC with fluorescence detection: a single-laboratory validation study.
Mycotoxins are toxic secondary metabolites of fungal origin, the major mycotoxins of food concern are aflatoxins and ochratoxin A. Due to the wide range of matrices susceptible to mycotoxin contamination, the possible co-occurrence, and the very wide range of concentration, validated versatile multi-mycotoxin and multi-matrix methods are strongly requested. A reversed phase HPLC method for the simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika was set up. Three bulk samples were prepared according to commercial availability, one for paprika and for baby foods, two different bulks were set, a corn based and a multi-cereal based baby food. A single-laboratory validation was performed, for each investigated level ten analyses were performed, relative standard deviations of repeatability (RSD(r)) and recovery factors were calculated; RSD(r) values ranged from 2% to 10% for AFB(1) and from 3% to 10% for OTA, while the recovery factors ranged from 86% to 96% for AFB(1) and from 77% to 96% for OTA. The checked compliance of the RSD(r) and recovery with the values reported in the current EU Regulations confirmed the fitting for purpose of the method. Limit of detection and LoQ values of the method were respectively 0.002 and 0.020 μg/kg for AFB(1) and 0.012 and 0.080 μg/kg for OTA in baby foods; and 0.002 and 0.200 μg/kg for AFB(1) and 0.012 and 0.660 μg/kg for OTA in paprika. The current method represents a good example of the possibility of a multi-mycotoxin and/or a multi-matrix analysis depending on the laboratory research or official control purposes.
Department of Veterinary Public Health and Food Safety, Italian National Institute for Health, Rome, Italy. carlo.brera@iss.it
C
C
Brera
F
F
Debegnach
B
B
De Santis
E
E
Pannunzi
C
C
Berdini
E
E
Prantera
E
E
Gregori
M
M
Miraglia
D000348:Aflatoxins / Q000032:analysis* / Q000737:chemistry; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D007223:Infant; D057230:Limit of Detection; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors
D000348:Aflatoxins; D009793:Ochratoxins; C025589:ochratoxin A