Mucin (MUC5AC) expression by lung epithelial cells cultured in a microfluidic gradient device.
We have developed a microfluidic gradient device for controlling mucin gene expression of NCI-H292 epithelial cells derived from lung tissues. We hypothesized that gradient profiles would control mucin gene expression of lung epithelial cells. However, it was not possible to generate various stable gradient profiles using conventional culture methods. To address this limitation, we used a microfluidic gradient device to create various gradient profiles (i.e. non-linear, linear, and flat) in a temporal and spatial manner. NCI-H292 lung epithelial cells were exposed to concentration gradients of epidermal growth factor in a microfluidic gradient device with continuous medium perfusion. We demonstrated an effect of gradient profiles on mucin expression of lung epithelial cells cultured in the microfluidic gradient device. It was revealed that NCI-H292 lung epithelial cells exposed to the flat gradient profile of the epidermal growth factor exhibited high expression of mucin as compared with cells exposed to non-linear and linear gradient profiles. Therefore, this microfluidic gradient device could be a potentially useful tool for regulating the mucin expression of lung epithelial cells exposed to chemokine gradient profiles.
Department of Bionano Engineering, Hanyang University, Ansan, Korea.
Su Hwan
SH
Kim
Jin Hyun
JH
Kang
Il Yup
IY
Chung
Bong Geun
BG
Chung
D004815:Epidermal Growth Factor / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009077:Mucins / Q000235:genetics* / Q000378:metabolism
D009077:Mucins; D004815:Epidermal Growth Factor