Identification of proanthocyanidin dimers and trimers, flavone C-Glycosides, and antioxidants in Ficus deltoidea , a malaysian herbal tea.
Phenolic compounds in an aqueous infusion of leaves of Ficus deltoidea (Moraceae), a well-known herbal tea in Malaysia, were analyzed by HPLC coupled to photodiode array and fluorescence detectors and an electrospray ionization tandem mass spectrometer. Following chromatography of extracts on a reversed phase C(12) column, 25 flavonoids were characterized and/or tentatively identified with the main constituents being flavan-3-ol monomers, proanthocyanidins, and C-linked flavone glycosides. The proanthocyanidins were dimers and trimers comprising (epi)catechin and (epi)afzelechin units. No higher molecular weight proanthocyanidin polymers were detected. The antioxidant activity of F. deltoidea extract was analyzed using HPLC with online antioxidant detection. This revealed that 85% of the total antioxidant activity of the aqueous F. deltoidea infusion was attributable to the flavan-3-ol monomers and the proanthocyanidins.
Plant Products and Human Nutrition Group, Graham Kerr Building, College of Medical, Veterinary and Life Sciences, University of Glasgow , Glasgow G12 8QQ, United Kingdom.
Maizatul Hasyima
MH
Omar
William
W
Mullen
Alan
A
Crozier
D000975:Antioxidants / Q000032:analysis*; D001628:Beverages; D001669:Biochemical Phenomena; D002851:Chromatography, High Pressure Liquid; D019281:Dimerization; D030681:Ficus / Q000737:chemistry*; D047309:Flavones / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D008296:Malaysia; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D044945:Proanthocyanidins / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D000975:Antioxidants; D047309:Flavones; D006027:Glycosides; D010936:Plant Extracts; D044945:Proanthocyanidins