Lipoic acid suppresses compound 48/80-induced anaphylaxis-like reaction.
Alpha-lipoic acid (LA), a naturally occurring dithiol compound, is an essential cofactor in metabolic reactions involved in energy utilization. LA improves glycemic control, reduces diabetic polyneuropathies, atherosclerosis, and allergic inflammation. The effects of LA on mast cell-mediated anaphylactic reactions, however, are unknown. LA dose-dependently inhibited systemic and passive cutaneous anaphylaxis-like reactions in mice induced by compound 48/80, a condensation product of N-methyl-p-methoxyphenethylamine and formaldehyde. Pretreatment with LA, prior to induction of the systemic anaphylaxis-like reaction with compound 48/80, reduced plasma histamine levels in a dose-dependent manner. In our in vitro study, LA decreased histamine release from rat peritoneal mast cells (RPMCs) triggered by compound 48/80. Moreover, an increase in calcium uptake activated by compound 48/80 was inhibited by LA. LA also significantly elevated intracellular cyclic adenosine-3',5' monophosphate (cAMP) levels in RPMCs. This inhibition of mediator release from RPMCs may be due to inhibition of calcium uptake and augmentation of intracellular cAMP levels. Based on these results, we suggest that LA may be a potential remedy for allergy-related diseases.
Department of Anatomy, Chonbuk National University Medical School, Institute for Medical Sciences, Chonbuk National University, Jeonju, Korea.
Yun Ho
YH
Choi
Ok Hee
OH
Chai
Eui-Hyeog
EH
Han
Su-Young
SY
Choi
Hyoung Tae
HT
Kim
Chang Ho
CH
Song
Int Immunopharmacol. 2004 Oct;4(10-11):1431-6
15313440
Cell Res. 2005 Oct;15(10):811-6
16246270
Biochim Biophys Acta. 2009 Oct;1790(10):1149-60
19664690
Agents Actions. 1981 Apr;11(1-2):51-4
6166166
Pharmacol Rep. 2005 Sep-Oct;57(5):570-7
16227639
Nature. 2008 Jul 24;454(7203):445-54
18650915
Gen Pharmacol. 1998 Nov;31(5):715-9
9809467
Biochem Mol Med. 1997 Jun;61(1):107-13
9232205
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3978-82
6204334
Biol Pharm Bull. 2004 Jun;27(6):929-31
15187450
Immunopharmacology. 1988 Sep-Oct;16(2):107-13
2462554
Biol Pharm Bull. 2006 Jul;29(7):1360-5
16819169
Biol Pharm Bull. 1994 May;17(5):732-4
7522732
J Allergy Clin Immunol. 2004 Aug;114(2):429-35
15316528
Ann Allergy. 1986 Jun;56(6):464-9
2424349
Exp Dermatol. 2010 Feb;19(2):117-22
19889021
Int Immunopharmacol. 2008 Sep;8(9):1207-15
18602066
Eur J Immunol. 2010 Jul;40(7):1843-51
20583030
J Immunol. 1988 Jan 15;140(2):571-9
2447182
Immunopharmacology. 1989 Nov-Dec;18(3):177-86
2481655
J Physiol. 1993 Jun;465:359-86
8229840