Chiral separation, determination of absolute configuration, and high-performance liquid chromatography detection of enantiomeric 3-hydroxyhexadecanoyl-CoA.
The enoyl-coenzyme A (CoA) hydratase catalyzes the hydration of 2-enoyl-CoA to yield 3-hydroxyacyl-CoA in mitochondrial and peroxisomal β-oxidation. However, the stereospecificities of these hydratases differ from each other. To provide clear evidence of the stereospecificities of hydratases, the absolute configuration of 3-hydroxyhexadecanoyl-CoAs was determined, and they were subjected to a high-performance liquid chromatography (HPLC) using a chiral separation column. The retention time of 3(R)-hydroxyhexadecanoyl-CoA was shorter than that of 3(S)-hydroxyhexadecanoyl-CoA. The HPLC analysis carried out using a chiral separation column is considered to be useful for the study of enoyl-CoA hydratase.
School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan. tsuchida@hoku-iryo-u.ac.jp
Shirou
S
Tsuchida
Koutarou
K
Kawamoto
Kana
K
Nunome
Naoya
N
Hamaue
Shu-Ping
SP
Hui
Tsuyoshi
T
Murai
Takashi
T
Aoki
Takao
T
Kurosawa
D000214:Acyl Coenzyme A / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013237:Stereoisomerism
D000214:Acyl Coenzyme A; C048143:3-hydroxyhexadecanoyl-coenzyme A