Preliminary study of the inflammatory response to subcutaneous implantation of three root canal sealers.
OBJECTIVE
To evaluate the kinetics of the inflammatory tissue response to three root canal sealers using a physicochemical method for quantification of the enhanced vascular permeability and histopathological analysis.


METHODS
Twenty-eight male Wistar rats randomly assigned to four groups according to the evaluation periods (1, 3, 7 and 14 days) were used to assess the vascular permeability and histopathological reaction to RoekoSeal, AH Plus and Sealapex (new formulation) sealers, using saline and Chloropercha as negative and positive controls, respectively. Seven rats were sacrificed per period. The biocompatibility of the sealers was evaluated spectrophotometrically and histopathologically.


RESULTS
At day 14, Sealapex produced significantly more inflammatory exudate than AH Plus and RoekoSeal (P < 0.05); however, there was no significant difference between AH Plus and RoekoSeal (P > 0.05). Sealapex (new formulation) was the most irritating sealer, producing severe inflammation with the presence of multinucleated giant cells. RoekoSeal was the most biocompatible sealer, producing the least amount of inflammatory exudate.


CONCLUSIONS
RoekoSeal root canal sealer was biocompatible when implanted in connective tissue.
Department of Endodontics, School of Dentistry, San Luis Potosi University, San Luis Potosi, Mexico.
D
D
Silva-Herzog
T
T
Ramírez
J
J
Mora
A J
AJ
Pozos
L A B
LA
Silva
R A B
RA
Silva
P
P
Nelson-Filho
D000818:Animals; D001453:Balsams; D002126:Calcium Hydroxide; D002199:Capillary Permeability / Q000187:drug effects*; D003738:Dental Cements; D004338:Drug Combinations; D004487:Edema / Q000139:chemically induced; D004853:Epoxy Resins; D005549:Foreign-Body Reaction / Q000139:chemically induced* / Q000276:immunology; D006171:Gutta-Percha; D020007:Implants, Experimental; D007249:Inflammation / Q000139:chemically induced* / Q000276:immunology; D007279:Injections, Subcutaneous; D008297:Male; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials; D012459:Salicylates; D018709:Statistics, Nonparametric; D013997:Time Factors; D015034:Zinc Oxide
D001453:Balsams; D003738:Dental Cements; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; C534916:epoxy resin-based root canal sealer; C465381:roeko seal; C055601:Sealapex; C029585:Kloroperka; D006171:Gutta-Percha; D002126:Calcium Hydroxide; D015034:Zinc Oxide