Role of thiol-disulfide system in mechanisms of functional changes in neutrophils under conditions of oxidative stress.
We studied the state of the thiol-disulfide system (contents of reduced and oxidized glutathione, their ratio, and concentrations of protein SH-groups and protein-bound glutathione) and functional properties of neutrophils (production of hydroxyl radicals, IL-8, and TNF-α and myeloperoxidase activity) from healthy donors under conditions of oxidative stress in vitro induced by H(2)O(2)in a final concentration of 200 μM and from patients with community-acquired pneumonia. We evaluated the role of reduced and protein-bound glutathione in the regulation of functional state of blood neutrophils from patients with community-acquired pneumonia and during oxidative stress in vitro under conditions cell incubation with N-ethylmaleimide or 1,4-dithioerythritolsulfhydryl, the blocker and protector of sulfhydryl groups, respectively.
Department of Biochemistry and Molecular Biology, Siberian State Medical University, Federal Agency for Health Care and Social Development, Tomsk, Russia.
E A
EA
Stepovaya
G V
GV
Petina
T V
TV
Zhavoronok
N V
NV
Ryazanceva
V V
VV
Ivanov
T S
TS
Ageeva
F F
FF
Tetenev
V V
VV
Novitsky
D000328:Adult; D017714:Community-Acquired Infections / Q000097:blood / Q000378:metabolism; D005033:Ethylmaleimide; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D011014:Pneumonia / Q000097:blood / Q000378:metabolism*; D011490:Protein Disulfide Reductase (Glutathione) / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D009195:Peroxidase; D011490:Protein Disulfide Reductase (Glutathione); D005978:Glutathione; D005033:Ethylmaleimide