Explicit analytic approximations for time-dependent solutions of the generalized integrated Michaelis-Menten equation.
Various explicit reformulations of time-dependent solutions for the classical two-step irreversible Michaelis-Menten enzyme reaction model have been described recently. In the current study, I present further improvements in terms of a generalized integrated form of the Michaelis-Menten equation for computation of substrate or product concentrations as functions of time for more real-world, enzyme-catalyzed reactions affected by the product. The explicit equations presented here can be considered as a simpler and useful alternative to the exact solution for the generalized integrated Michaelis-Menten equation when fitted to time course data using standard curve-fitting software.
Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia. marko.golicnik@mf.uni-lj.si
Marko
M
Goličnik
D000465:Algorithms; D055162:Biocatalysis; D004798:Enzymes / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D012984:Software; D012996:Solutions / Q000737:chemistry; D013997:Time Factors
D004798:Enzymes; D012996:Solutions