Combined association of CCR2-V64I and MCP-1-2518A/G polymorphisms with generalised aggressive periodontitis in Chinese.
OBJECTIVE
to examine the possible association of CCR2-V64I and MCP-1-2518A/G polymorphisms with generalised aggressive periodontitis (GAgP) in the Chinese population.


METHODS
one hundred and twenty-four GAgP patients and 94 healthy subjects were included in the study. A peripheral blood sample was obtained from each subject and genomic DNA was isolated. Gene polymorphisms of CCR2-V64I and MCP-1-2518A/G were analysed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.


RESULTS
a possible combined effect of CCR2-V64I and MCP-1-2518A/G was observed in the female GAgP patients, as the odds ratio for VV genotype (CCR2) and G+ genotype ( MCP-1) was 0.2 (P = 0.023). Individuals carrying VV genotype and G+ genotype were at reduced risk for GAgP. A possible combined effect of genotype and smoking was observed in the male GAgP patients, as the odds ratio for VV genotype (CCR2) and smoking, or G+ genotype (MCP-1) and smoking were 7.4 (P = 0.022) and 4.9 (P = 0.030), respectively.


CONCLUSIONS
the combined association of CCR2-V64I and MCP-1-2518A/G polymorphisms may play an important role in determining GAgP susceptibility in Chinese females. A possible combined effect of genotype and smoking on GAgP susceptibility was suggested in males.
Departement of Periodontology, Peking Unviersity School and Hospital of Stomatology, Beijing, P.R. China.
Xiao Ling
XL
Zhu
Huan Xin
HX
Meng
Li
L
Xu
Li
L
Zhang
Zhi Bin
ZB
Chen
Dong
D
Shi
Xiang Hui
XH
Feng
D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics*; D019943:Amino Acid Substitution; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics*; D016009:Chi-Square Distribution; D002681:China; D018389:Codon, Nonsense; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D008297:Male; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D054390:Receptors, CCR2 / Q000235:genetics*; D012307:Risk Factors; D012737:Sex Factors; D012907:Smoking
C111835:CCL2 protein, human; C515334:CCR2 protein, human; D018932:Chemokine CCL2; D018389:Codon, Nonsense; D054390:Receptors, CCR2