Hypersensitive response and acyl-homoserine lactone production of the fire blight antagonists Erwinia tasmaniensis and Erwinia billingiae.
Fire blight caused by the Gram-negative bacterium Erwinia amylovora can be controlled by antagonistic microorganisms. We characterized epiphytic bacteria isolated from healthy apple and pear trees in Australia, named Erwinia tasmaniensis, and the epiphytic bacterium Erwinia billingiae from England for physiological properties, interaction with plants and interference with growth of E. amylovora. They reduced symptom formation by the fire blight pathogen on immature pears and the colonization of apple flowers. In contrast to E. billingiae, E. tasmaniensis strains induced a hypersensitive response in tobacco leaves and synthesized levan in the presence of sucrose. With consensus primers deduced from lsc as well as hrpL, hrcC and hrcR of the hrp region of E. amylovora and of related bacteria, these genes were successfully amplified from E. tasmaniensis DNA and alignment of the encoded proteins to other Erwinia species supported a role for environmental fitness of the epiphytic bacterium. Unlike E. tasmaniensis, the epiphytic bacterium E. billingiae produced an acyl-homoserine lactone for bacterial cell-to-cell communication. Their competition with the growth of E. amylovora may be involved in controlling fire blight.
Max-Planck-Institut für Zellbiologie, Ladenburg and Julius Kühn Institut für Pflanzenschutz in Obst- und Weinbau, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.
Vladimir
V
Jakovljevic
Susanne
S
Jock
Zhiqiang
Z
Du
Klaus
K
Geider
D054742:Acyl-Butyrolactones / Q000276:immunology*; D000906:Antibodies; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D004885:Erwinia / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D044084:Erwinia amylovora / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D027845:Malus / Q000382:microbiology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D031989:Pyrus / Q000382:microbiology; D016415:Sequence Alignment; D014026:Nicotiana / Q000276:immunology / Q000382:microbiology
D054742:Acyl-Butyrolactones; D000906:Antibodies; D001426:Bacterial Proteins
Microbiology (Reading). 2001 Nov;147(Pt 11):2951-9
11700346
FEMS Microbiol Lett. 2002 Jun 4;211(2):247-52
12076820
Appl Environ Microbiol. 1988 Nov;54(11):2798-802
16347778
Syst Appl Microbiol. 1998 Aug;21(3):384-97
9779605
Proteomics. 2004 Oct;4(10):3177-86
15378709
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:377-83
10319458
Appl Environ Microbiol. 2003 Jan;69(1):679-82
12514060
Appl Environ Microbiol. 2001 Feb;67(2):575-85
11157219
J Bacteriol. 1992 May;174(9):2785-96
1314801
Annu Rev Phytopathol. 1998;36:227-48
15012499
Phytopathology. 2002 Nov;92(11):1202-9
18944246
Int J Syst Bacteriol. 1990 Oct;40(4):379-83
2275853
Phytopathology. 2007 Feb;97(2):244-9
18944382
FEMS Microbiol Lett. 1999 Feb 1;171(1):1-9
9987836
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:899-905
10319516
J Bacteriol. 2005 May;187(9):3206-13
15838048
J Bacteriol. 1995 Nov;177(21):6201-10
7592386
Microbes Infect. 2003 Nov;5(13):1177-87
14623013
Appl Environ Microbiol. 2001 Jan;67(1):284-92
11133457
Plant Dis. 1998 Feb;82(2):158-164
30856793
Environ Microbiol. 2003 Oct;5(10):1016-21
14510856
Annu Rev Phytopathol. 2003;41:455-82
12730390
Plant Dis. 2002 Mar;86(3):304-311
30818612
Int J Syst Evol Microbiol. 2006 Dec;56(Pt 12):2937-2943
17159002
Appl Environ Microbiol. 1992 Nov;58(11):3522-6
1482178
J Bacteriol. 1997 Sep;179(18):5655-62
9294418
FEMS Microbiol Lett. 2007 Jan;266(1):34-41
17092294
J Clin Invest. 2003 Nov;112(9):1291-9
14597753
Microbiology (Reading). 1997 Dec;143 ( Pt 12):3703-3711
9421896
Microbes Infect. 2001 Oct;3(12):985-95
11580985
Environ Microbiol. 2008 Sep;10(9):2211-22
18462403
FEMS Microbiol Lett. 2004 Dec 15;241(2):179-83
15598530
Mol Microbiol. 2001 Sep;41(5):999-1014
11555282
Phytopathology. 1999 Dec;89(12):1162-8
18944640
Phytopathology. 2004 Dec;94(12):1286-94
18943698