A nonsense mutation in a putative sulphate transporter gene results in low phytic acid in barley.
Low phytic acid grains can provide a solution to dietary micronutrient deficiency and environmental pollution. A low phytic acid 1-1 (lpa1-1) barley mutant was identified using forward genetics and the mutant gene was mapped to chromosome 2HL. Comparative genomic analysis revealed that the lpa1-1 gene was located in the syntenic region of the rice Os-lpa-MH86-1 gene on chromosome 4. The gene ortholog of rice Os-lpa-MH86-1 (designated as HvST) was isolated from barley using polymerase chain reaction and mapped to chromosome 2HL in a doubled haploid population of Clipper×Sahara. The results demonstrate the collinearity between the rice Os-lpa-MH86-1 gene and the barley lpa1-1 region. Sequence analysis of HvST revealed a single base pair substitution (C→T transition) in the last exon of the gene in lpa1-1 (M422), which resulted in a nonsense mutation. These results will facilitate our understanding of the molecular mechanisms controlling the low phytic acid phenotype and assist in the development of a diagnostic marker for the selection of the lpa1-1 gene in barley.
State Key Lab of Rice Biology and Key Lab of the Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, People's Republic of China.
Hongxia
H
Ye
Xiao-Qi
XQ
Zhang
Sue
S
Broughton
Sharon
S
Westcott
Dianxing
D
Wu
Reg
R
Lance
Chengdao
C
Li
D027321:Anion Transport Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018389:Codon, Nonsense / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics; D010833:Phytic Acid / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013431:Sulfates / Q000378:metabolism*
D027321:Anion Transport Proteins; D018389:Codon, Nonsense; D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant; D013431:Sulfates; D010833:Phytic Acid
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