Preparation and preliminary cytocompatibility of magnesium doped apatite cement with degradability for bone regeneration.
In the present study, we fabricated magnesium doped apatite cement (md-AC) with rapid self-setting characteristic by adding the mixed powders of magnesium oxide and calcium dihydrogen phosphate (MO-CDP) into hydroxyapatite cement (HAC). The results revealed that the md-AC with 50 wt% MO-CDP could set within 6 min and the compression strength could reach 51 MPa after setting for 1 h, indicating that the md-AC had highly initial mechanical strength. The degradability of the md-AC in Tris-HCl solution increased with the increase of MO-CDP amount, and the weight loss ratio of md-AC with 50 wt% MO-CDP was 57.5 wt% after soaked for 12 weeks. Newly flake-like apatite could be deposited on the md-AC surfaces after soaked in simulated body fluid (SBF) for 7 days. Cell proliferation ratio of MG(63) cells on md-AC was obviously higher than that of HAC on days 4 and 7. The cells with normal phenotype spread well on the md-AC surfaces and attached intimately with the substrate, and alkaline phosphatase (ALP) activity of the cells on md-AC significantly improved compared with HAC on day 7. The results demonstrate that the md-AC has a good ability to support cell proliferation and differentiation, and indicate a good cytocompatibility.
Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China. jingxionglu@yahoo.cn
Jingxiong
J
Lu
Jie
J
Wei
Yonggang
Y
Yan
Hong
H
Li
Junfeng
J
Jia
Shicheng
S
Wei
Han
H
Guo
Tiqiao
T
Xiao
Changsheng
C
Liu
D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials; D001843:Bone Cements / Q000737:chemistry*; D001861:Bone Regeneration; D001842:Bone and Bones; D002454:Cell Differentiation; D049109:Cell Proliferation; D019245:Compressive Strength; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry; D007477:Ions; D008274:Magnesium / Q000737:chemistry*; D008956:Models, Chemical; D010641:Phenotype; D011208:Powders; D013314:Stress, Mechanical; D013997:Time Factors; D014961:X-Ray Diffraction
D001031:Apatites; D001672:Biocompatible Materials; D001843:Bone Cements; D006882:Hydroxyapatites; D007477:Ions; D011208:Powders; D000469:Alkaline Phosphatase; D008274:Magnesium
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