[Effect of lyophilized powder of Sini powder on 5-HT content and 5-HT(1A) receptor in drosophila heads].
OBJECTIVE
To unveil the mechanism of sleep-improving effect of lyophilized powder of Sini powder on drosophila.


METHODS
Drosophila melanogaster whose strain were wild Canton S, unmated, were collected daring 12 h after their emergencing, then they were anaesthetized by small flow of CO2 and divided into different tubes due to their sex. Both male and female flies were distributed randomly into three groups as blank, control and administration, 32 flies each group. The content of 5-HT in the brain was estimated was estimated with ELISA and had a comparison of the expression of 5-HT(1A) receptor in brain, using real-time quantitative PCR.


RESULTS
Concentration of 5-HT in brain was increased in administration group, and had a significant difference to control group. The expression of 5-HT(1A) receptor was also up-regulated after administrated of Sini powder.


CONCLUSIONS
Sleep-improving effect of Sini powder on drosophila has a contribution to the increasing of 5-HT and 5-HT(1A) receptor in brain.
Heilongfiang University of Chinese Medicine, Harbin 150040, China.
Yuping
Y
Li
Ruixin
R
Xu
Tingli
T
Li
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D004331:Drosophila melanogaster; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005612:Freeze Drying; D011208:Powders; D044282:Receptor, Serotonin, 5-HT1A / Q000032:analysis*; D012701:Serotonin / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D011208:Powders; D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin