High-resolution fiber optic microscopy with fluorescent contrast enhancement for the identification of axillary lymph node metastases in breast cancer: a pilot study.
This prospective pilot study evaluates the potential of high-resolution fiber optic microscopy (HRFM) to identify lymph node metastases in breast cancer patients. 43 lymph nodes were collected from 14 consenting breast cancer patients. Proflavine dye was topically applied to lymph nodes ex vivo to allow visualization of nuclei. 242 images were collected at 105 sites with confirmed histopathologic diagnosis. Quantitative statistical features were calculated from images, assessed with one-way ANOVA, and were used to develop a classification algorithm with the goal of objectively discriminating between normal and metastatic tissue. A classification algorithm using mean image intensity and skewness achieved sensitivity of 79% (27/34) and specificity of 77% (55/71). This study demonstrates the technical feasibility and diagnostic potential of HRFM with fluorescent contrast in the ex vivo evaluation of lymph nodes from breast cancer patients.
Kelsey J
KJ
Rosbach
Dongsuk
D
Shin
Timothy J
TJ
Muldoon
Mohammad A
MA
Quraishi
Lavinia P
LP
Middleton
Kelly K
KK
Hunt
Funda
F
Meric-Bernstam
Tse-Kuan
TK
Yu
Rebecca R
RR
Richards-Kortum
Wei
W
Yang
J Clin Oncol. 2002 Oct 15;20(20):4242-8
12377968
JAMA. 1996 Dec 11;276(22):1818-22
8946902
Eur J Radiol. 2009 May;70(2):227-31
19269762
Lancet. 1997 Jun 28;349(9069):1864-7
9217757
Ann Surg Oncol. 2010 Apr;17(4):953-8
20127185
Mutat Res. 1991 Sep;258(2):123-60
1881402
Opt Express. 2007 Dec 10;15(25):16413-23
19550931
Br J Surg. 2001 Jun;88(6):891
11412274
N Engl J Med. 1998 Oct 1;339(14):941-6
9753708
Br J Surg. 2001 Jan;88(1):114-7
11136322
Ann Surg Oncol. 2003 Apr;10(3):248-54
12679309
AJR Am J Roentgenol. 2006 May;186(5):1342-8
16632729
Am J Clin Pathol. 1973 May;59(5):666-73
4702684
J Clin Oncol. 2009 Jan 20;27(3):390-7
19064976
J Natl Cancer Inst. 1999 Feb 17;91(4):368-73
10050871
Phys Med Biol. 2009 Jun 7;54(11):3291-301
19430111
Arch Surg. 1999 Jul;134(7):764-7
10401830
N Engl J Med. 1998 Oct 1;339(14):990-5
9753717
Eur J Nucl Med Mol Imaging. 2004 Jun;31 Suppl 1:S97-102
15133635
Gastrointest Endosc. 2008 Oct;68(4):737-44
18926182
Ann Surg Oncol. 2010 Aug;17(8):2132-7
20155400
Nat Methods. 2005 Dec;2(12):941-50
16299479
Breast Cancer Res Treat. 2003 May;79(1):47-57
12779081
Opt Lett. 1993 Apr 15;18(8):565
19802201
Cancer. 2002 Aug 25;96(4):225-31
12209664
J Natl Cancer Inst. 2010 Mar 17;102(6):410-25
20190185