Determination of stimulants and narcotics as well as their in vitro metabolites by online CE-ESI-MS.
A simple, rapid and sensitive CE-ESI-MS method for the simultaneous analysis of seven stimulants and narcotics (amphetamine, ephedrine, methadone, pethidine, tetracaine, codeine and heroin) was developed. The CE-ESI-MS experimental conditions were optimized as follows: 20 mmol/L ammonium acetate with pH 9.0 as running buffer, the separation voltage of 22 kV and the sheath liquid of isopropanol/water (1:1 v/v) containing 7.5 mmol/L acetic acid with 3.0 μL/min flow rate. Under the optimized conditions, the stimulants and narcotics were well separated within 4.6 min using a 70-cm length fused-silica capillary (50 μm id). The detection limits (S/N=3) of the CE-ESI-MS analysis were in the range of 0.40-1.0 ng/mL. Method repeatability of intra-day and inter-day was satisfactory. The recoveries obtained from the analysis of spiked urine samples were between 84.1 and 108%. The developed method was successfully applied for the simultaneous analysis of methadone, pethidine and codeine and their in vitro metabolites.
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, PR China.
Minghua
M
Lu
Qin
Q
Li
Yongquan
Y
Lai
Lan
L
Zhang
Bin
B
Qiu
Guonan
G
Chen
Zongwei
Z
Cai
D019840:2-Propanol / Q000737:chemistry; D000661:Amphetamine / Q000032:analysis; D000818:Animals; D003061:Codeine / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D004809:Ephedrine / Q000032:analysis; D003932:Heroin / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008297:Male; D008614:Meperidine / Q000032:analysis; D008691:Methadone / Q000032:analysis; D008862:Microsomes, Liver / Q000737:chemistry*; D009294:Narcotics / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013748:Tetracaine / Q000032:analysis
D009294:Narcotics; D013748:Tetracaine; D003932:Heroin; D008614:Meperidine; D000661:Amphetamine; D004809:Ephedrine; D019840:2-Propanol; D008691:Methadone; D003061:Codeine