Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea.
In methanogenic archaea growing on H(2) and CO(2) the first step in methanogenesis is the ferredoxin-dependent endergonic reduction of CO(2) with H(2) to formylmethanofuran and the last step is the exergonic reduction of the heterodisulfide CoM-S-S-CoB with H(2) to coenzyme M (CoM-SH) and coenzyme B (CoB-SH). We recently proposed that in hydrogenotrophic methanogens the two reactions are energetically coupled via the cytoplasmic MvhADG/HdrABC complex. It is reported here that the purified complex from Methanothermobacter marburgensis catalyzes the CoM-S-S-CoB-dependent reduction of ferredoxin with H(2). Per mole CoM-S-S-CoB added, 1 mol of ferredoxin (Fd) was reduced, indicating an electron bifurcation coupling mechanism: 2H(2) + Fd(OX) + CoM-S-S-CoB-->Fd(red)(2-) + CoM-SH + CoB-SH + 2H(+). This stoichiometry of coupling is consistent with an ATP gain per mole methane from 4 H(2) and CO(2) of near 0.5 deduced from an H(2)-threshold concentration of 8 Pa and a growth yield of up to 3 g/mol methane.
Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.
Anne-Kristin
AK
Kaster
Johanna
J
Moll
Kristian
K
Parey
Rudolf K
RK
Thauer
D002245:Carbon Dioxide / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015080:Mesna / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*; D017013:Methanobacteriaceae / Q000378:metabolism*; D008795:Metronidazole; D010084:Oxidation-Reduction; D010769:Phosphothreonine / Q000031:analogs & derivatives / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004220:Disulfides; D005288:Ferredoxins; C049349:7-mercaptoheptanoylthreonine phosphate; D010769:Phosphothreonine; D008795:Metronidazole; D002245:Carbon Dioxide; D006859:Hydrogen; D015080:Mesna; D008697:Methane
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