Complete nucleotide sequence and genome organisation of grapevine Bulgarian latent virus.
The complete genome sequence of grapevine Bulgarian latent virus (GBLV) has been determined. RNA-1 (7,452 nt in length) contains a single ORF of 6,285 nt, encoding a polyprotein with conserved motifs characteristic of the viral protease cofactor (Prot-cofact), the NTP-binding protein (NTP), the cysteine-like protease (Cyst-Prot) and the RNA-dependent RNA polymerase (RdRp) of members of the order Picornavirales and show high aa sequence identity with blackcurrant reversion virus (BRV, 64%). RNA-2 (5,821 nt) contains a single ORF of 4,500 nt, encoding a polyprotein in which the conserved motifs of the movement protein (MP) and coat protein (CP) have been identified. The GBLV CP aa sequence shows highest homology with that of blueberry leaf mottle virus (BLMoV, 68%). Both RNAs have a poly(A) tail and a NCR at the 3' and 5' termini, respectively. The results of this study confirm the classification of GBLV as a member of a distinct species in subgroup C of the genus Nepovirus.
Istituto Agronomico Mediterraneo di Bari, Valenzano, Bari, Italy. elbeaino@iamb.it
Toufic
T
Elbeaino
Michele
M
Digiaro
Frida
F
Fallanaj
Slobodan
S
Kuzmanovic
Giovanni Paolo
GP
Martelli
D001483:Base Sequence; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins