Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain.
The diagnostic performance of an ELISA for the detection of antibodies to the small ruminant lentiviruses (SRLVs) maedi-visna virus and caprine arthritis-encephalitis virus in milk and corresponding blood samples was evaluated in 50 sheep. The agreement between ELISA results in blood and milk was 90 per cent, and the κ value was 0.79. In addition, a serological survey in the central zone of Spain was performed using milk samples from 413 animals (250 sheep and 163 goats) from 12 flocks/herds. All flocks/herds had some animals that were positive for SRLV. Among the animals, 60.0 per cent of the sheep and 8.0 per cent of the goats tested were seropositive. Each sample was also tested using a PCR technique, which increased the percentage of positive animals detected. Using a combination of ELISA and PCR gave a total of 72.2 per cent of sheep and 28.8 per cent of goats positive for SRLV.
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro, 28040 Madrid, Spain. nuria.barquero@vet.ucm.es
N
N
Barquero
A
A
Arjona
A
A
Domenech
C
C
Toural
A
A
de las Heras
J F
JF
Fernández-Garayzabal
J A
JA
Ruiz-Santa Quiteria
E
E
Gomez-Lucia
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis*; D006041:Goats; D016086:Lentivirus / Q000276:immunology / Q000302:isolation & purification; D016180:Lentivirus Infections / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D008892:Milk / Q000821:virology*; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000097:blood / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis*; D013030:Spain; D014790:Visna-maedi virus / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral