Linear correlation between 1H and 13C chemical shifts of ferriheme proteins and model ferrihemes.
The (1)H{(13)C} HMQC experiment at natural-abundance (13)C provides a very useful way of determining not only (1)H but also (13)C chemical shifts of most heme substituents, without isotopic labeling of the hemin. This is true both in model low-spin ferriheme complexes and in low-spin ferriheme proteins, even when the proton resonances are buried in the protein diamagnetic region, because the carbon shifts are much larger than the proton shifts. In addition, in many cases, the protohemin methyl cross peaks are fairly linearly related to each other, with the slope of the correlation, δ(C)/δ(H), being approximately -2.0 for most low-spin ferriheme proteins. The reasons why this should be the case, and when it is not, are discussed.
Department of Chemistry and Biochemistry, The University of Arizona, P.O. Box 210041, Tucson, Arizona 85721-0041, United States.
Fei
F
Yang
Tatiana K
TK
Shokhireva
F Ann
FA
Walker
D002247:Carbon Isotopes / Q000032:analysis / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011522:Protons
D002247:Carbon Isotopes; D006420:Hemeproteins; D011522:Protons; D006427:Hemin; D006859:Hydrogen
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