Identification of cancer stem cells derived from a canine lung adenocarcinoma cell line.
Accumulating evidence supporting the cancer stem cell (CSC) hypothesis is based on the finding that tumors contain a small population of self-renewing cells that generate differentiated progeny and thereby contribute to tumor heterogeneity. CSCs are reported to exist in several human cancers, yet only a few reports demonstrate the existence of CSCs in primary lung cancer in dogs. In this study, the authors established a cancer cell line derived from a canine primary lung adenocarcinoma and identified a side population (SP) of cells that displayed drug-resistant features. To confirm the characteristics of these SP cells, the authors investigated the tumorigenicity of the cells in vivo by using a nude mouse xenograft model. Only 100 SP cells were able to give rise to new tumors, giving a 10-fold enrichment over the main population (MP) of cells, suggesting that these cells have the cancer-initiating ability of CSCs. Further studies characterizing CSCs in canine lung adenocarcinoma might contribute to the elucidation of the mechanisms of tumorigenesis and to the establishment of novel therapeutic strategies.
Veterinary Teaching Hospital, Azabu University, Sagamihara, Japan.
Y
Y
Nemoto
T
T
Maruo
T
T
Sato
T
T
Deguchi
T
T
Ito
H
H
Sugiyama
T
T
Ishikawa
H
H
Madarame
T
T
Watanabe
T
T
Shida
H
H
Sahara
D000230:Adenocarcinoma / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D008175:Lung Neoplasms / Q000473:pathology / Q000662:veterinary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000473:pathology*; D014183:Transplantation, Heterologous / Q000662:veterinary