Separation and determination of β-casomorphins by using glass microfluidic chip electrophoresis together with laser-induced fluorescence detection.
A simple, reliable and reproducible method for the separation and determination of five β-casomorphins (β-CMs, namely TPGN, PGPI, TPGI, TPGP and TPPG) based on glass microfluidic chip electrophoresis and laser-induced fluorescence detection is first described in here. The microfluidic chip electrophoresis and laser-induced fluorescence detection system consisted of a home-made glass "double-T" microchip and a simple LIF detector with excitation and emission wavelengths of 473 and 525 nm, respectively. Fluorescein isothiocyanate (FITC) was used as the precolumn derivatization reagent to label fluorophore on five β-CMs, and the optimum conditions of FITC-derivatization reaction and MCE separation were investigated in detail. Under optimum conditions, five β-CMs were completely separated and detected within 30 min with a detection limit of 18.7-75.1 nmol/L and an RSD (n=5) of 3.0-5.9%, respectively. The proposed method has been successfully used to detect β-CMs in real cheese sample with a recovery of 89-109%, suggesting that our method is sensitive and reliable. These features, as well as its low cost, operation convenience, stability and reusability, make it a promising alternative to β-CMs detection methods.
Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, P. R. China.
ZongWen
Z
Wang
WenJian
W
Wang
Wei
W
Wang
LiangJun
L
Xu
GuoNan
G
Chen
FengFu
F
Fu
D000701:Analgesics, Opioid / Q000737:chemistry* / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004723:Endorphins / Q000737:chemistry* / Q000302:isolation & purification*; D005453:Fluorescence; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
D000701:Analgesics, Opioid; D004723:Endorphins; C021968:beta-casomorphins