High-density EEG recordings of the freely moving mice using polyimide-based microelectrode.
Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a noninvasive brain monitoring tool in cognitive neuroscience as well as a diagnostic tool for epilepsy and sleep disorders in neurology. However, the underlying mechanism of EEG rhythm generation is still under the veil. Recently introduced polyimide-based microelectrode (PBM-array) for high resolution mouse EEG is one of the trials to answer the neurophysiological questions on EEG signals based on a rich genetic resource that the mouse model contains for the analysis of complex EEG generation process. This application of nanofabricated PBM-array to mouse skull is an efficient tool for collecting large-scale brain activity of transgenic mice and accommodates to identify the neural correlates to certain EEG rhythms in conjunction with behavior. However its ultra-thin thickness and bifurcated structure cause a trouble in handling and implantation of PBM-array. In the presented video, the preparation and surgery steps for the implantation of PBM-array on a mouse skull are described step by step. Handling and surgery tips to help researchers succeed in implantation are also provided.
Center for Neural Science , Korea Institute of Science and Technology.
Mina
M
Lee
Dongwook
D
Kim
Hee-Sup
HS
Shin
Ho-Geun
HG
Sung
Jee Hyun
JH
Choi
D000818:Animals; D004569:Electroencephalography / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008839:Microelectrodes; D036103:Nanotechnology / Q000379:methods; D012117:Resins, Synthetic / Q000737:chemistry*
D012117:Resins, Synthetic; C029945:polyimide resin
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:2934-6
19964604
J Neurophysiol. 2010 Sep;104(3):1825-34
20610789