Quantitative analysis of promoter methylation of the EDNRB gene in gastric cancer.
Hypermethylation has been shown in the promoter region of the endothelin receptor B (EDNRB) gene in several human tumors. However, its role in gastric cancer formation is still unclear. In this study, the methylation status of the EDNRB gene in paired gastric cancer tissues and adjacent normal tissues from 96 patients was detected quantitatively using pyrosequencing. The results showed the methylation of promoter of EDNRB gene in gastric cancer (50.42 ± 9.03%) was significantly higher than in adjacent normal tissues (6.47 ± 2.98%) (P < 0.01). Among 96 tumor tissues, promoter hypermethylation of the EDNRB gene was correlated with tumor infiltration (T1: 47.4 ± 7.31% T2:48.2 ± 9.17% T3:52.9 ± 6.48% T4:53.2 ± 10.45%), lymph node metastasis (N0:45.4 ± 6.99% N1:49.0 ± 9.10% N2:52.0 ± 8.40% N3:53.7 ± 9.92%), and distant metastasis (M0:48.9 ± 6.99% M1:53.9 ± 11.98%) (P < 0.05), but it was not associated with other clinicopathological characteristics. In addition, the treatment of the human gastric cancer cell line, SGC-7901, with demethylation agent can restore the expression of EDNRB. Our results suggest that promoter hypermethylation of EDNRB gene is highly prevalent in gastric cancer, which may play a role in the pathogenesis of gastric cancer. Futhermore, hypermethylation of EDNRB gene was remarkably related to infiltration and metastasis of gastric cancer and may attribute to the tumor progression.
Department of Laparoscopic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, Hubei 430022, People's Republic of China.
Kaixiong
K
Tao
Chuanqing
C
Wu
Ke
K
Wu
Wei
W
Li
Gaoxiong
G
Han
Xiaoming
X
Shuai
Guobin
G
Wang
D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017466:Receptors, Endothelin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*
D014408:Biomarkers, Tumor; D017466:Receptors, Endothelin
Cancer Res. 2006 May 15;66(10):5495-503
16707479
Cell. 1994 Dec 30;79(7):1257-66
8001158
Br J Cancer. 2008 Dec 2;99(11):1802-7
19002170
Int J Dev Biol. 2003 Jun;47(5):315-25
12895026
Indian J Med Res. 2010 Sep;132:295-302
20847376
Lancet Oncol. 2003 Jun;4(6):351-8
12788407
Nat Rev Genet. 2006 Jan;7(1):21-33
16369569
Oncol Rep. 2006 Feb;15(2):507-11
16391877
Adv Cancer Res. 1998;72:141-96
9338076
Folia Biol (Praha). 2010;56(5):195-200
21138650
J Clin Pathol. 2003 Jan;56(1):52-5
12499435
Oncol Rep. 2006 Jan;15(1):167-72
16328051
Nat Rev Cancer. 2005 Mar;5(3):223-31
15719030
Cancer Res. 2007 Aug 1;67(15):7095-105
17671176
Cancer Res. 2001 Apr 15;61(8):3225-9
11309270
Dis Esophagus. 2009;22(1):55-61
18564167
Nat Rev Cancer. 2004 Dec;4(12):988-93
15573120
Cancer Lett. 2007 Feb 8;246(1-2):139-48
16581180
Science. 2001 Aug 10;293(5532):1068-70
11498573
Cancer Res. 1999 Mar 1;59(5):1090-5
10070967
Int J Cancer. 2002 Apr 10;98(5):651-5
11920632
Oncogene. 2001 Mar 22;20(12):1525-8
11313896
CA Cancer J Clin. 2005 Mar-Apr;55(2):74-108
15761078
Cancer Res. 2004 Mar 15;64(6):1975-86
15026333
J Invest Dermatol. 1999 Jun;112(6):925-32
10383740
Hum Mol Genet. 2001 Apr 15;10(9):903-10
11309363