Community structure and antibiotic production of Streptomyces nodosus bioreactors cultured in liquid environments.
Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside the alginate matrix and within multicellular projections on the surface of the capsule. The periphery of these projections had erect and branched hyphae, morphologically identical to aerial hyphae. Antibiotic production from immobilized organisms was assessed using conditioned culture medium to eliminate the emergence of a free-dwelling population. These organisms sporulated with reduced antibiotic production compared with free-dwelling cultures. The commitment to sporulate was independent of a surface but dependent on community size and nutritional status. This is the first report of the sporulation of S. nodosus in liquid cultures and description of the multicellular community the organism adopts at a solid-liquid interface.
University of Western Sydney, School of Natural Sciences, Parramatta Campus, Locked Bag 1797, Penrith South DC 1797 NSW, Australia.
Tanya
T
Pereira
Jasmina
J
Nikodinovic
Chojin
C
Nakazono
Gary R
GR
Dennis
Kevin D
KD
Barrow
Jo-Anne
JA
Chuck
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D003470:Culture Media
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