The septic milieu triggers expression of spliced tissue factor mRNA in human platelets.
BACKGROUND
Activated platelets have previously-unrecognized mechanisms of post-transcriptional gene expression that may influence hemostasis and inflammation. A novel pathway involves splicing of pre-mRNAs in resting platelets to mature, translatable mRNAs in response to cellular activation.


OBJECTIVE
We asked if bacterial products and host agonists present in the septic milieu induce tissue factor pre-mRNA splicing in platelets from healthy subjects. In parallel, we asked if spliced tissue factor (TF) mRNA is present in platelets from septic patients in a proof-of-principle analysis.


METHODS
TF pre-mRNA and mRNA expression patterns were characterized in platelets from septic patients and in platelets isolated from healthy subjects activated with bacteria, toxins and inflammatory agonists. Procoagulant activity was also measured.


CONCLUSIONS
Live bacteria, staphylococcal α-toxin and lipopolysaccharide (LPS) induced TF pre-mRNA splicing in platelets isolated from healthy subjects. Toxin-stimulated platelets accelerated plasma clotting, a response that was blocked by a previously-characterized splicing inhibitor and by an anti-tissue factor antibody. Platelets from septic patients expressed spliced TF mRNA, whereas it was absent from unselected and age-matched control subjects. Tissue factor-dependent procoagulant activity was elevated in platelets from a subset of septic patients. Thus, bacterial and host factors induce splicing of TF pre-mRNA, expression of TF mRNA and tissue factor-dependent clotting activity in human platelets. TF mRNA is present in platelets from some septic patients, indicating that it may be a marker of altered platelet phenotype and function in sepsis and that splicing pathways are induced in this syndrome.
Division of General Internal Medicine, University of Utah School of Medicine, Salt Lake City, UT 84132, USA. matthew.rondina@hsc.utah.edu
M T
MT
Rondina
H
H
Schwertz
E S
ES
Harris
B F
BF
Kraemer
R A
RA
Campbell
N
N
Mackman
C K
CK
Grissom
A S
AS
Weyrich
G A
GA
Zimmerman
D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism*; D017931:DNA Primers; D006801:Humans; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*; D018805:Sepsis / Q000378:metabolism*
D017931:DNA Primers; D012333:RNA, Messenger
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