Revolutionizing membrane protein overexpression in bacteria.
The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein overexpression in bacteria. Recent studies have shown that it is feasible to (i) engineer or select for E. coli strains with strongly improved membrane protein overexpression characteristics, (ii) use bacteria other than E. coli for the expression of membrane proteins, (iii) engineer or select for membrane protein variants that retain functionality but express better than the wild-type protein, and (iv) express membrane proteins using E. coli-based cell-free systems.
Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
Susan
S
Schlegel
Mirjam
M
Klepsch
Dimitra
D
Gialama
David
D
Wickström
Dirk Jan
DJ
Slotboom
Jan-Willem
JW
de Gier
D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002474:Cell-Free System; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D008565:Membrane Proteins; D011994:Recombinant Proteins
Q Rev Biophys. 1999 Nov;32(4):285-307
11194567
FEBS Lett. 2004 Apr 30;564(3):289-93
15111111
Biochem J. 2008 Jan 1;409(1):193-8
17910535
J Mol Biol. 1996 Jul 19;260(3):289-98
8757792
J Cell Mol Med. 2007 Mar-Apr;11(2):224-38
17488474
Biochim Biophys Acta. 2003 Feb 17;1610(1):23-36
12586376
Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14371-6
18796603
Biochem J. 2007 Apr 1;403(1):183-7
17176254
FEBS J. 2005 Dec;272(23):6024-38
16302967
Protein Sci. 2009 Feb;18(2):372-83
19165721
FEBS Lett. 2001 Jul 13;501(1):1-5
11457446
Biochim Biophys Acta. 2003 Feb 17;1610(1):97-108
12586384
Nature. 2001 Oct 25;413(6858):852-6
11677609
Science. 2005 May 27;308(5726):1321-3
15919996
Biochim Biophys Acta. 2008 May;1778(5):1237-50
18295592
Nat Methods. 2006 Apr;3(4):303-13
16554836
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14808-13
18812512
J Struct Funct Genomics. 2007 Dec;8(4):167-72
17985211
Protein Sci. 2007 May;16(5):966-76
17456747
Science. 2005 Feb 25;307(5713):1317-21
15731457
Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6656-61
19366667
Biotechnol Bioeng. 2009 Feb 1;102(2):357-67
18828176
Protein Expr Purif. 2009 Nov;68(1):28-33
19524676
Science. 2002 Nov 29;298(5599):1790-3
12459590
Protein Sci. 2009 May;18(5):1121-7
19388077
Annu Rev Microbiol. 2005;59:329-55
16153172
Biochemistry. 2008 Nov 18;47(46):12175-84
18950196
Protein Sci. 2008 Apr;17(4):673-80
18305199
Microb Cell Fact. 2008 Apr 04;7:10
18394159
Science. 2006 Sep 8;313(5792):1441-3
16960007
Nature. 2009 May 21;459(7245):379-85
19458714
Protein Sci. 2005 Oct;14(10):2638-53
16195551
J Proteome Res. 2008 Aug;7(8):3535-42
18557639
Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004
18024586
Protein Sci. 2005 Aug;14(8):2011-7
15987891
J Virol. 2007 Apr;81(7):3181-6
17229695
Mol Cell Proteomics. 2008 Nov;7(11):2246-53
18603642
Microb Cell Fact. 2009 Jan 27;8:9
19173718
Biochim Biophys Acta. 2003 Feb 17;1610(1):37-45
12586377
Proc Natl Acad Sci U S A. 2008 Jan 22;105(3):877-82
18192400
Protein Expr Purif. 2003 Dec;32(2):221-31
14965767
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3603-8
17360689
Structure. 2008 Jun;16(6):897-905
18547522
FEBS Lett. 2001 Oct 26;507(2):220-4
11684102
Protein Sci. 2006 Sep;15(9):2178-89
16943447
J Mol Biol. 1968 Dec;38(3):421-6
4887878
Nat Biotechnol. 2001 Aug;19(8):751-5
11479568
Methods Enzymol. 1990;185:60-89
2199796
J Bacteriol. 1992 Jan;174(2):619-22
1729251
Trends Biotechnol. 1997 Apr;15(4):135-40
9131833
J Bacteriol. 2009 Jan;191(1):42-51
18931129
Biotechniques. 2006 Mar;40(3):355-64
16568824
Curr Opin Biotechnol. 2005 Oct;16(5):546-51
16143505
Protein Eng. 1995 Jul;8(7):693-8
8577697
Nat Methods. 2005 Jul;2(7):507-9
15973420
Annu Rev Biochem. 2004;73:1019-49
15189166
FEBS J. 2005 Jan;272(1):28-36
15634329
Proteomics. 2008 Oct;8(19):3933-46
18763710
Eur J Biochem. 2004 Feb;271(3):568-80
14728684
Protein Sci. 2005 Jan;14(1):148-58
15608119
Trends Biotechnol. 2006 Aug;24(8):364-71
16820235
Protein Sci. 1998 Apr;7(4):1029-38
9568909
Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10744-9
18664584
Protein Sci. 2005 Dec;14(12):3048-56
16260761
Eur J Biochem. 2002 Jan;269(1):82-92
11784301
Biochem J. 1996 Aug 1;317 ( Pt 3):891-9
8760379
Protein Expr Purif. 2005 May;41(1):27-37
15802218
Protein Sci. 2008 Oct;17(10):1857-63
18593817