Lung-derived soluble mediators are pathogenic in ventilator-induced lung injury.
Ventilator-induced lung injury (VILI) due to high tidal volume (V(T)) is associated with increased levels of circulating factors that may contribute to, or be markers of, injury. This study investigated if exclusively lung-derived circulating factors produced during high V(T) ventilation can cause or worsen VILI. In isolated perfused mouse lungs, recirculation of perfusate worsened injury (compliance impairment, microvascular permeability, edema) induced by high V(T). Perfusate collected from lungs ventilated with high V(T) and used to perfuse lungs ventilated with low V(T) caused similar compliance impairment and permeability and caused a dose-dependent decrease in transepithelial electrical resistance (TER) across rat distal lung epithelial monolayers. Circulating soluble factors derived from the isolated lung thus contributed to VILI and had deleterious effects on the lung epithelial barrier. These data demonstrate transferability of an injury initially caused exclusively by mechanical ventilation and provides novel evidence for the biotrauma hypothesis in VILI. Mediators of the TER decrease were heat-sensitive, transferable via Folch extraction, and (following ultrafiltration, 3 kDa) comprised both smaller and larger molecules. Although several classes of candidate mediators, including protein cytokines (e.g., tumor necrosis factor-α, interleukin-6, macrophage inflammation protein-1α) and lipids (e.g., eicosanoids, ceramides, sphingolipids), have been implicated in VILI, only prostanoids accumulated in the perfusate in a pattern consistent with a pathogenic role, yet cyclooxygenase inhibition did not protect against injury. Although no single class of factor appears solely responsible for the decrease in barrier function, the current data implicate lipid-soluble protein-bound molecules as not just markers but pathogenic mediators in VILI.
Programme in Physiology & Experimental Medicine, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.
Thomas
T
Jaecklin
Doreen
D
Engelberts
Gail
G
Otulakowski
Hugh
H
O'Brodovich
Martin
M
Post
Brian P
BP
Kavanagh
D000818:Animals; D016207:Cytokines / Q000378:metabolism; D017097:Electric Impedance; D004848:Epithelium / Q000473:pathology; D066298:In Vitro Techniques; D008055:Lipids / Q000302:isolation & purification; D008168:Lung / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010477:Perfusion; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism; D011489:Protein Denaturation; D051381:Rats; D012995:Solubility; D013696:Temperature; D014462:Ultrafiltration; D055397:Ventilator-Induced Lung Injury / Q000473:pathology*
D016207:Cytokines; D008055:Lipids; D011453:Prostaglandins; D011451:Prostaglandin-Endoperoxide Synthases