A fungal enzyme with the ability of aflatoxin B₁ conversion: purification and ESI-MS/MS identification.
Armillariella tabescens, a Chinese edible and medicinal fungus, whose multienzyme exist ability of AFB(1)-converting, and ADTZ (aflatoxin-detoxizyme) had previously purified from the A. tabescens multienzyme monitored by AFB(1) conversion(.) However, the enzyme now confirmed an oxidase and renamed aflatoxin-oxidase (AFO). In this paper, AFO was purified by an economical and practical three-step procedure monitored by AFB(1) conversion. And ESI-MS/MS analysis was done for identification of AFO. The following database searching (Protein Blast on NCBI) results did not show any homologous oxidase protein, which implied that AFO was mostly a new oxidase differing from other reported aflatoxin-converting enzymes such as fungal laccase and horse radish peroxidase. HPTLC analysis of the purified AFO activity suggested that the enzyme reacted at the bisfuran ring of AFB(1) which was the key toxic structure. Therefore, all these investigations implied a new choice for biodegradation of aflatoxin in foods and feeds with the practical application of AFO.
Institute of Microbial Technology, Jinan University, Guangzhou City, Guangdong Province 510632, China.
Hong
H
Cao
Daling
D
Liu
Xuemei
X
Mo
Chunfang
C
Xie
Dongsheng
D
Yao
D016604:Aflatoxin B1 / Q000378:metabolism*; D000363:Agaricales / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010449:Peptide Mapping; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D005656:Fungal Proteins; D016604:Aflatoxin B1; D010088:Oxidoreductases