Analysis of active ricin and castor bean proteins in a ricin preparation, castor bean extract, and surface swabs from a public health investigation.
In late February 2008, law enforcement officials in Las Vegas, Nevada, discovered in a hotel room, a copy of The Anarchist Cookbook, suspected castor beans and a "white powder" thought to be a preparation of ricin. Ricin is a deadly toxin from the seed of the castor bean plant (Ricinus communis). The United States regulates the possession, use, and transfer of ricin and it is the only substance considered a warfare agent in both the Chemical and the Biological Weapons Conventions. Six samples obtained from the hotel room were analyzed by laboratories at the Centers for Disease Control and Prevention using a panel of biological and mass spectrometric assays. The biological assays (real time-PCR, time resolved fluorescence and cytotoxicity) provided presumptive evidence of active ricin in each of the samples. This initial screen was followed by an in-depth analysis using a novel, state-of-the-art mass spectrometry-based ricin functional assay and high sensitivity tandem mass spectrometry for protein identification. Mass spectrometric analysis positively identified ricin and confirmed that in each of the samples it was enzymatically active. The tandem mass spectrometry analysis used here is the most selective method available to detect ricin toxin. In each sample, ricin was unequivocally identified along with other R. communis plant proteins, including the highly homologous protein RCA120. Although database searches using tandem mass spectra acquired from the samples indicated that additional controlled substances were not present in these samples, the mass spectrometric results did provide extensive detail about the sample contents. To the best of our knowledge following a review of the available literature, this report describes the most detailed analysis of a white powder for a public health or forensic investigation involving ricin.
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
David M
DM
Schieltz
Sara C
SC
McGrath
Lisa G
LG
McWilliams
Jon
J
Rees
Michael D
MD
Bowen
John J
JJ
Kools
Leslie A
LA
Dauphin
Eduardo
E
Gomez-Saladin
Bruce N
BN
Newton
Heather L
HL
Stang
Michael J
MJ
Vick
Jerry
J
Thomas
James L
JL
Pirkle
John R
JR
Barr
D002367:Ricinus communis / Q000737:chemistry* / Q000235:genetics; D002619:Chemical Warfare Agents / Q000032:analysis*; D017931:DNA Primers; D015342:DNA Probes; D006801:Humans; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry* / Q000235:genetics; D037121:Plant Lectins / Q000235:genetics; D010940:Plant Proteins / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D040901:Proteomics; D011634:Public Health; D012276:Ricin / Q000032:analysis* / Q000235:genetics
D002619:Chemical Warfare Agents; D017931:DNA Primers; D015342:DNA Probes; D010936:Plant Extracts; D037121:Plant Lectins; D010940:Plant Proteins; C017707:Ricinus communis agglutinin-1; D012276:Ricin