Breast fine-needle aspiration malondialdehyde deoxyguanosine adduct in breast cancer.
This study has analysed the generation of 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine adduct [M₁dG], a biomarker of oxidative stress and lipid peroxidation, in breast fine-needle aspirate samples of 22 patients with breast cancer, at different clinical stages, in respect to 13 controls. The multivariate analysis show that M(1)dG adduct was higher in cases than in controls (Mean Ratio (MR) = 5.26, 95% CI = 3.16-8.77). Increased M₁dG was observed in women with a tumour grade 3 and a pathological diameter 2 (MR = 7.61, 95% CI = 3.91-14.80 and MR = 5.75, 95% CI = 3.13-10.59, respectively). A trend with increasing tumour grade and pathological diameter was present (MR = 1.98, 95% CI = 1.57-2.50 and MR = 2.44, 95% CI = 1.71-3.48, respectively). Not significant effects of age and smoking habit were found (MR = 1.58, 95% CI = 0.92-2.72 and MR = 1.68, 95% CI 0.88-3.20, respectively). An increment over the background frequency of M₁dG can contribute to breast cancer development. Increasing severity of breast tumour can influence DNA damage level.
Cancer Risk Factor Branch, ISPO-Cancer Prevention and Research Institute, Florence, Italy. m.peluso@ispo.toscana.it
Marco
M
Peluso
Armelle
A
Munnia
Gabriella G
GG
Risso
Sandra
S
Catarzi
Sara
S
Piro
Marcello
M
Ceppi
Roger W
RW
Giese
Beniamino
B
Brancato
D000367:Age Factors; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D044963:Biopsy, Fine-Needle; D001943:Breast Neoplasms / Q000453:epidemiology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D004249:DNA Damage; D003849:Deoxyguanosine / Q000032:analysis / Q000737:chemistry; D005260:Female; D006801:Humans; D007558:Italy; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000737:chemistry; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D011684:Purine Nucleosides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012307:Risk Factors; D012720:Severity of Illness Index; D012907:Smoking; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
C491625:3-(2'-deoxy-beta-D-erythro-pentofuranosyl)pyrimido(1,2-alpha)purin-10(3H)-one; D014408:Biomarkers, Tumor; D018736:DNA Adducts; D011684:Purine Nucleosides; D008315:Malondialdehyde; D004247:DNA; C427203:calf thymus DNA; D003849:Deoxyguanosine
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