NdhP and NdhQ: two novel small subunits of the cyanobacterial NDH-1 complex.
The subunit composition of the NAD(P)H dehydrogenase complex of Thermosynechococcus elongatus was analyzed by different types of mass spectrometry. All 15 known subunits (NdhA-NdhO) were identified in the purified NDH-1L complex. Moreover, two additional intact mass tags of 4902.7 and 4710.5 Da could be assigned after reannotation of the T. elongatus genome. NdhP and NdhQ are predicted to contain a single transmembrane helix each, and homologues are apparent in other cyanobacteria. Additionally, ndhP is present in some cyanophages in a cluster of PSI genes and exhibits partial similarity to NDF6, a subunit of the plant NDH-1 complex.
Department of Plant Biochemistry, Ruhr-University Bochum, D-44780 Bochum, Germany. marc.m.nowaczyk@rub.de
Marc M
MM
Nowaczyk
Hannes
H
Wulfhorst
Christopher M
CM
Ryan
Puneet
P
Souda
Huamin
H
Zhang
William A
WA
Cramer
Julian P
JP
Whitelegge
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009245:NADH Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism* / Q000502:physiology; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; D021122:Protein Subunits; D009245:NADH Dehydrogenase
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