[Use of perfluorocarbon emulsions for administration of photosensitizing preparations into bone marrow stem cells].
It has been shown that, upon incubation of mouse bone marrow stem cells (BMSC) in vitro with the nanoparticles of perfluorocarbon (PFC) emulsion stabilized by proxanol 268, these nanoparticles penetrate into cells and stay there for a long time (up to 20 days of observation). It has been found that, under in vitro conditions, mouse BMSC loaded with the nanoparticles of both the original emulsion and the emulsion preliminarily incubated with radachlorine do not differ from control stem cells in the rate of division, stretching on a plastic support, and the formation of a monolayer. It has been shown that the exposure to laser radiation of BMSC incubated with the nanoparticles of a PFC emulsion preliminarily incubated with radachlorine under in vitro conditions leads to the death of these cells due to the destruction of the cell membrane. The treatment with laser radiation of BMSC incubated with the nanoparticles of the starting PFC emulsion (without preliminarily incubation with radachlorine) causes no death of these cells. It has been shown in in vivo experiments that, when transplanted to the organism of a recipient mouse, BMSC of a donor mouse incubated with the nanoparticles of a PFC emulsion preliminarily incubated with radachlorine retain their functional activity, in particular the ability to migrate in the animal body. In this case, radachlorine contained in these stem cells retains its major function, to induce the death of stem cells by the action of laser radiation due to the destruction of the cell membrane. The observation period after the transplantation was 5-7 days.
A A
AA
Temnov
A N
AN
Sklifas
A V
AV
Tereshchenko
Iu A
IuA
Belyĭ
N B
NB
Lyskov
N I
NI
Kukushkin
D000818:Animals; D001692:Biological Transport; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D002470:Cell Survival / Q000528:radiation effects; D002735:Chlorophyllides; D004337:Drug Carriers; D004655:Emulsions; D005466:Fluorocarbons; D007834:Lasers; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D053758:Nanoparticles; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000378:metabolism; D011060:Poloxalene; D011166:Porphyrins / Q000008:administration & dosage* / Q000378:metabolism
D002735:Chlorophyllides; D004337:Drug Carriers; D004655:Emulsions; D005466:Fluorocarbons; D017319:Photosensitizing Agents; D011166:Porphyrins; C062985:phytochlorin; D011060:Poloxalene