Deepening into the proteome of maize cells habituated to the cellulose biosynthesis inhibitor dichlobenil.
Cellulose biosynthesis inhibitors, such as dichlobenil (DCB), have become a valuable tool for the analysis of structural and compositional plasticity of plant cell walls. By stepwise increasing the concentration of DCB in the culture medium, we obtained maize cells able to cope with DCB through the acquisition of a modified cell wall in which cellulose was partially replaced by a more extensive network of feruloylated arabinoxylans. Recently we demonstrated that the expression of several Cellulose Synthase and phenylpropanoid-related genes is altered in DCB-habituated cells. In addition, by using a proteomic approach we identified several proteins induced or repressed in DCB-habituated cells. After a more in-depth analysis, some new proteins induced (two inhibitors TAXI-IV, an α-1,4-glucan-protein synthase, and a pectinesterase inhibitor) or repressed (a chaperonin 60, a fructokinase-1 and a spermidine synthase 1) were identified, and their possible role in the context of DCB-habituation is discussed.
Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, León, Spain.
Hugo
H
Mélida
David
D
Caparrós-Ruiz
Jesús
J
Alvarez
José Luis
JL
Acebes
Antonio
A
Encina
D002478:Cells, Cultured; D002482:Cellulose / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D009570:Nitriles / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D003313:Zea mays / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
D009570:Nitriles; D010940:Plant Proteins; D020543:Proteome; D002482:Cellulose; C055869:dichlobanil
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