Plasmid-borne 16S rRNA methylase ArmA in aminoglycoside-resistant Klebsiella pneumoniae in Poland.
We characterized 17 clinical isolates of Klebsiella pneumoniae producing 16S rRNA methylase ArmA. The isolates originated in Poland from 2002 to May 2010 and encompassed four XbaI-PFGE clusters. All the isolates were resistant to amikacin, gentamicin and kanamycin (MIC range: 256-1024 mg l(-1)) and carried the armA gene on a large plasmid of approximately 90 or 130 kb in 15 and 2 isolates, respectively. The armA gene was found in a ~10 kb ClaI restriction fragment of the large plasmid and was flanked by the same elements as in Tn1548. All the isolates carried the bla(CTX-M) gene for a CTX-M-type extended-spectrum β-lactamase. Our results show that ArmA has disseminated horizontally among K. pneumoniae isolates in Poland on the ~90 kb plasmid of the pCTX-M3 family.
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Katarzyna
K
Zacharczuk
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Katarzyna
K
Piekarska
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Jolanta
J
Szych
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Marek
M
Jagielski
Departamento de Sanidad Animal, Facultad de Veterinaria and VISAVET (Centro de Vigilancia Sanitaria Veterinaria), Universidad Complutense de Madrid, 28040 Madrid, Spain.
Laura
L
Hidalgo
Departamento de Sanidad Animal, Facultad de Veterinaria and VISAVET (Centro de Vigilancia Sanitaria Veterinaria), Universidad Complutense de Madrid, 28040 Madrid, Spain.
Álvaro
Á
San Millán
Departamento de Sanidad Animal, Facultad de Veterinaria and VISAVET (Centro de Vigilancia Sanitaria Veterinaria), Universidad Complutense de Madrid, 28040 Madrid, Spain.
Belén
B
Gutiérrez
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Waldemar
W
Rastawicki
Departamento de Sanidad Animal, Facultad de Veterinaria and VISAVET (Centro de Vigilancia Sanitaria Veterinaria), Universidad Complutense de Madrid, 28040 Madrid, Spain.
Bruno
B
González-Zorn
Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Rafał
R
Gierczyński
D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D022761:Gene Transfer, Horizontal; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008745:Methylation; D008780:Methyltransferases / Q000096:biosynthesis* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D011044:Poland; D012329:RNA, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D001618:beta-Lactamases