Oily fraction of Semecarpus anacardium Linn nuts involves protein kinase C activation for its pro-inflammatory response.
The oily fraction (non polar fraction-NPF) of S. anacardium (SA) significantly increased the expression of protein kinase C-delta (PKC-delta) in macrophages in concentration dependent manner, which was similar to phorbol myristate acetate (PMA) response. Further, H-7 (1-(5-isoquinolinesulphonyl)-2-methylpiperazine), an inhibitor of PKC significantly inhibited this NPF mediated response in a concentration dependent manner. In the post treatment kinetics, H-7 showed this inhibition only up to 6 min post NPF/PMA addition, but in similar condition, quercetin, a flavone with reported antioxidant property, showed this inhibition only up to 2 min. The results clearly suggest that oily fraction of SA nuts enhances the expression of PKC protein, which may be responsible for its reported pro-inflammatory property.
Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. yaminiok@yahoo.com
Yamini B
YB
Tripathi
Nidhi
N
Pandey
Deepshikha
D
Tripathi
Pratibha
P
Tripathi
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009754:Nuts / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051745:Protein Kinase C-delta / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D031023:Semecarpus / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000975:Antioxidants; D002273:Carcinogens; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011794:Quercetin; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051745:Protein Kinase C-delta; D013755:Tetradecanoylphorbol Acetate