[Expression of human testis development related gene 1 in testicular cancer detected by tissue microarray].
OBJECTIVE
To investigate the protein expression of human testis development related gene 1 (TDRG1) in human testicular cancer and its pathological significance.


METHODS
The expression levels of TDRG1 were detected in the testis tissues of testicular cancer patients and normal men by tissue microarray and immunohistochemistry, and the results were analyzed.


RESULTS
Immunohistochemistry exhibited positive expression of the TDRG1 protein in the testis of 73.3% (11/15) of the normal men, 83.3% (10/12) of the patients with embryonal carcinoma, 80.0% (8/10) of those with yolk sac tumor, 26.9% (7/26) of those with seminoma, and 57.1% (4/7) of those with teratoma. The expression levels of TDRG1 in the testis tissues of the seminoma and teratoma groups were shown to be significantly lower than that of the normal control (P < 0.01 and P < 0.05), but those of the embryonal carcinoma and yolk sac tumor groups exhibited no significant differences from that of the latter (P > 0.05).


CONCLUSIONS
The significantly reduced expression of the TDRG1 protein in patients with seminoma or teratoma indicates that TDRG1 may be a candidate cancer suppressor gene.
Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China. chenhouyang2007@163.com
Hou-Yang
HY
Chen
Jia-Ming
JM
Wen
Xiao-Wang
XW
Xiao
Dong-Jie
DJ
Li
Xiao-Liang
XL
Guo
Zhi
Z
Long
Ying-Bo
YB
Dai
Yu-Xin
YX
Tang
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
D018398:Homeodomain Proteins