Bio-functionalization of metal-organic frameworks by covalent protein conjugation.
Bioconjugation of functional proteins onto metal-organic frameworks (MOFs) has been achieved using activation of pendent linking groups of the organic linkers on the surface of MOFs. Fluorescent microscopy revealed successful conjugation of an enhanced fluorescent protein onto MOFs. In addition, Candida-antarctica-lipase-B-conjugated MOFs showed no loss of enantioselectivity and activity in transesterification of (±)-1-phenylethanol.
Department of Chemistry, Center for NanoBio Applied Technology, Institute of Basic Sciences, Sungshin Women's University, Seoul 136-742, Korea.
Suhyun
S
Jung
Youngmee
Y
Kim
Sung-Jin
SJ
Kim
Tae-Hwan
TH
Kwon
Seong
S
Huh
Seongsoon
S
Park
D055162:Biocatalysis; D005656:Fungal Proteins; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
D005656:Fungal Proteins; D009942:Organometallic Compounds; D011506:Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica