[Determination of nicacid and its metabolites in human plasma by HPLC-MS/MS].
OBJECTIVE
To develop a sensitive HPLC-MS/MS method for the determination of nicacid and its metabolites in human plasma.


METHODS
The assay was conducted with an API 3000 LC-MS/MS system comprising of a Genimi C18 column (50 x 3.00 mm, 3 microm), and an eluate of 0.1% acetic acid-methanol-isopropyl alcohol (98: 1:1), and flow rate was 0.2 mL/min. Acetonitrile was used to precipitate protein from the plasma samples. The loading samples contained the residue from the supernatant that were dissolved in the eluate solution and rinsed by dichloromethane was used as the loading samples. The ion pairs of m/z 124.1-->80.0, m/z 123.1-->80.0, m/z 181.1-->135.0 and m/z 138.1-->92.0 were used to quantify nicacid, niacinamide, nicotinuric acid and 6-methyl nicotinic acid (IS), respectively.


RESULTS
The standard curves of nicacid, niacinamide and nicotinuric were linear in the range of 1.25-320 microg/L, 1.25-1280 microg/L and 1.25-1280 microg/L, respectivly. All with a low determination limits of 1.25 microg/L and a less than 9% within-day and inter-day RSD. The recovery rates reached 89% to 105%.


CONCLUSIONS
The method is simple, rapid, sensitive, and suitable for the determination of nicacid and its metabolites in human plasma.
Department of Clinical Pharmacology, West China Hospital, Sichuan University, Chengdu 610041, China.
Lei
L
Wang
Yong-ping
YP
Qin
Xiao-li
XL
Gong
Feng
F
Nan
Jin
J
Xiang
Qin
Q
Yu
Mao-zhi
MZ
Liang
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009525:Niacin / Q000097:blood*; D009536:Niacinamide / Q000097:blood*; D009539:Nicotinic Acids / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods
D009539:Nicotinic Acids; D009536:Niacinamide; D009525:Niacin; C002477:nicotinuric acid