A simple double-bead sandwich assay for protein detection in serum using UV-vis spectroscopy.
In this study a double-bead sandwich assay, employing magnetic nanoparticles and gold nanoparticles is proposed. The magnetic nanoparticles allow specific capturing of the analyte in biological samples, while the optical properties of the gold nanoparticles provide the signal transduction. We demonstrated that a major improvement in the assay sensitivity was obtained by selecting an optimal gold nanoparticle size (60 nm). A detection limit of 5-8 ng/mL, a sensitivity of 0.6-0.8 (pg/mL)(-1) and a dynamic range of 3 orders of magnitude were achieved without any further amplification using the detection of prostate specific antigen in serum as a model system. The proposed assay has the ability to be easily implemented within a microfluidic device for point-of-care applications whereby the readout can be executed by a fast and cheap optical measurement.
imec, SSET/Functional Nanosystems, Leuven, Belgium. Hilde.Jans@imec.be
Hilde
H
Jans
Karolien
K
Jans
Pieter-Jan
PJ
Demeyer
Karel
K
Knez
Tim
T
Stakenborg
Guido
G
Maes
Liesbet
L
Lagae
D001774:Blood Chemical Analysis; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*
D011506:Proteins; D006046:Gold