Passive optical separation and enrichment of cells by size difference.
A size-selective cell sorting microfluidic device that utilizes optical force is developed. The device consists of a three-dimensional polydimethylsiloxane microstructure comprised of two crossed microchannels in a three-dimensional configuration. A line shaped focused laser beam is used for automatic size-selective cell sorting in a continuous flow environment. As yeast cells in an aqueous medium are fed continuously into a lower channel, the line shaped focused laser beam is applied (perpendicular to the direction of flow) at the junction of the two crossed channels. The scattering force of the laser beam was employed to push cells matching specific criteria upward from one channel to another. The force depends on the size of the cells, the laser power, and the fluid flow speed. The variation in size of yeast cells causes them to follow different routes at the intersection. For flow speeds below 30 μm∕s, all yeast cells larger than 3 μm were removed from the main stream. As a result, a high purity sample of small cells can be collected at the outlet of bottom channel.
Siew-Kit
SK
Hoi
Vuong Hoang
VH
Kim
Nguyen Manh
NM
Huy
Chorng-Haur
CH
Sow
Yueh-Sheng
YS
Ow
Andrew A
AA
Bettiol
Opt Express. 2004 Sep 20;12(19):4390-8
19483988
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15757-61
16236724
Electrophoresis. 2010 Apr;31(8):1366-75
20301125
Anal Chem. 2008 Aug 15;80(16):6365-72
18510341
Anal Chem. 2001 Oct 1;73(19):4682-7
11605847
Biomed Microdevices. 2008 Feb;10(1):55-63
17659444
Lab Chip. 2007 Nov;7(11):1461-8
17960272
Lab Chip. 2008 Feb;8(2):280-6
18231667
Biomed Microdevices. 2007 Oct;9(5):637-45
17530413
Electrophoresis. 2007 Dec;28(24):4511-20
18008312
Opt Lett. 2007 May 1;32(9):1144-6
17410263
J Biomed Opt. 2007 Sep-Oct;12(5):054017
17994905
Nature. 2003 Nov 27;426(6965):421-4
14647376
Opt Express. 2008 Mar 17;16(6):3712-26
18542466
Nat Biotechnol. 2005 Jan;23(1):83-7
15608628
Anal Chem. 2008 May 1;80(9):3135-43
18363383
Opt Lett. 1986 May 1;11(5):288
19730608
Anal Chem. 2005 Feb 1;77(3):933-7
15679363