Birthdating studies reshape models for pituitary gland cell specification.
The intermediate and anterior lobes of the pituitary gland are derived from an invagination of oral ectoderm that forms Rathke's pouch. During gestation proliferating cells are enriched around the pouch lumen, and they appear to delaminate as they exit the cell cycle and differentiate. During late mouse gestation and the postnatal period, anterior lobe progenitors re-enter the cell cycle and expand the populations of specialized, hormone-producing cells. At birth, all cell types are present, and their localization appears stratified based on cell type. We conducted a birth dating study of Rathke's pouch derivatives to determine whether the location of specialized cells at birth is correlated with the timing of cell cycle exit. We find that all of the anterior lobe cell types initiate differentiation concurrently with a peak between e11.5 and e13.5. Differentiation of intermediate lobe melanotropes is delayed relative to anterior lobe cell types. We discovered that specialized cell types are not grouped together based on birth date and are dispersed throughout the anterior lobe. Thus, the apparent stratification of specialized cells at birth is not correlated with cell cycle exit. Thus, the currently popular model of cell specification, dependent upon timing of extrinsic, directional gradients of signaling molecules, needs revision. We propose that signals intrinsic to Rathke's pouch are necessary for cell specification between e11.5 and e13.5 and that cell-cell communication likely plays an important role in regulating this process.
Department of Human Genetics, University of Michigan Medical School, 4909 Buhl Building, 1241 East Catherine Street, Ann Arbor, MI 48109-5618, USA. davissw@umich.edu
Shannon W
SW
Davis
Amanda H
AH
Mortensen
Sally A
SA
Camper
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism; D005865:Gestational Age; D051379:Mice; D008959:Models, Neurological; D010902:Pituitary Gland / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000196:embryology / Q000502:physiology; D010907:Pituitary Hormones / Q000096:biosynthesis; D011247:Pregnancy; D015398:Signal Transduction
D019485:Bone Morphogenetic Proteins; D010907:Pituitary Hormones; D005346:Fibroblast Growth Factors
Microsc Res Tech. 1997 Oct 15;39(2):138-49
9361265
J Biol Chem. 1996 Jul 5;271(27):15918-21
8663172
Dev Biol. 2007 Nov 15;311(2):396-407
17919533
Development. 1998 Dec;125(23):4835-40
9806931
Mol Endocrinol. 2000 Jan;14(1):137-46
10628753
Am J Anat. 1978 Oct;153(2):193-215
360813
PLoS One. 2009;4(3):e4815
19283075
Mol Endocrinol. 2007 Jun;21(6):1458-66
17426285
J Neurocytol. 1989 Jun;18(3):311-8
2746304
J Comp Neurol. 1985 Dec 22;242(4):611-31
4086673
Cell Tissue Res. 1990 Aug;261(2):333-8
2401005
Endocrinology. 2004 Sep;145(9):4134-43
15155578
Dev Biol. 2007 Apr 15;304(2):455-66
17367776
Curr Opin Genet Dev. 2002 Oct;12(5):534-39
12200158
Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16880-5
16272219
Endocrinology. 2007 Apr;148(4):1736-44
17218416
J Histochem Cytochem. 1994 Aug;42(8):1117-25
8027530
Anat Embryol (Berl). 2001 Apr;203(4):249-53
11396852
Dev Dyn. 2008 Apr;237(4):1006-20
18351662
Development. 2000 Oct;127(19):4265-75
10976057
Genes Dev. 1998 Jun 1;12(11):1691-704
9620855
Dev Biol. 2004 Jan 15;265(2):329-40
14732396
Dev Biol. 2008 Jul 15;319(2):248-57
18534570
Endocrinology. 2008 May;149(5):2159-67
18202140
Nature. 1995 Feb 2;373(6513):427-32
7830793
Dev Biol. 2002 Apr 15;244(2):319-28
11944940
Mol Endocrinol. 2005 Mar;19(3):698-710
15591534
Mol Cell Endocrinol. 1999 Apr 25;150(1-2):57-63
10411300
Genes Dev. 2003 Mar 15;17(6):738-47
12651892
Nat Genet. 2004 Mar;36(3):247-55
14981518
Anat Embryol (Berl). 2001 Dec;204(6):469-75
11876532
Stem Cells. 2009 May;27(5):1182-95
19418455
Mol Cell Biol. 2009 Apr;29(7):1895-908
19139274
Dev Biol. 1972 May;28(1):142-61
5041191
Dev Biol. 2007 May 1;305(1):145-60
17359964
Mol Endocrinol. 2006 Jun;20(6):1378-90
16556738
Dev Biol. 2010 Jan 15;337(2):313-23
19900438
Mol Endocrinol. 1994 Jul;8(7):878-85
7527122
Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2907-12
18287078
Development. 1998 Mar;125(6):1005-15
9463347
Development. 2000 Feb;127(3):483-92
10631169
Cell Tissue Res. 1991 Jan;263(1):41-7
1849046
Endocrinology. 2009 Feb;150(2):1061-8
18927214
Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16372-7
20805495
Endocrinology. 1982 May;110(5):1489-97
7075529
Front Biosci. 2007 Jan 01;12:125-34
17127288
Cell Tissue Res. 1982;224(3):563-77
7116413