High-speed focal modulation microscopy using acousto-optical modulators.
Focal Modulation Microscopy (FMM) is a single-photon excitation fluorescence microscopy technique which effectively rejects the out-of-focus fluorescence background that arises when imaging deep inside biological tissues. Here, we report on the implementation of FMM in which laser intensity modulation at the focal plane is achieved using acousto-optic modulators (AOM). The modulation speed is greatly enhanced to the MHz range and thus enables real-time image acquisition. The capability of FMM is demonstrated by imaging fluorescence labeled vasculatures in mouse brain as well as self-made tissue phantom.
Shau Poh
SP
Chong
Chee Howe
CH
Wong
Kit Fei
KF
Wong
Colin J R
CJ
Sheppard
Nanguang
N
Chen
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