Sensitive counting of viable Enterobacteriaceae in seawaters and relationship with fecal indicators.
A fluorescent in situ hybridization based assay was used to enumerate viable Enterobacteriaceae members in seawaters by solid phase cytometry. The method was specific, highly sensitive (1cell/100ml) and allowed the quantification of VNC Enterobacteriaceae cells during an osmotic stress. Investigations on contaminated coastal seawater revealed a strong correlation between Enterobacteriaceae counts and standard fecal indicators.
UPMC Univ Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/mer, France. baudart@obs-banyuls.fr
J
J
Baudart
A
A
Robyns
S
S
Peuchet
J L
JL
Drocourt
P
P
Lebaron
D058491:Bacterial Load / Q000379:methods*; D004755:Enterobacteriaceae / Q000302:isolation & purification* / Q000502:physiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D050296:Microbial Viability; D012623:Seawater / Q000382:microbiology*; D012680:Sensitivity and Specificity; D014873:Water Pollutants; D014876:Water Pollution
D014873:Water Pollutants