Highly sensitive DNA detection with a combination of 2 DNA-intercalating dyes for microchip electrophoresis.
A highly sensitive DNA detection method using a combination of ethidium bromide (EtBr) and SYBR Green II (SG II) for microchip electrophoresis was developed. By use of the combination of these intercalating DNA-staining dyes for microchip electrophoresis with Hitachi SV1100 system, the fluorescence intensities corresponding to DNA fragments were obviously increased over those obtained with EtBr only, with accuracy of DNA sizing and quantification. The detection limit with EtBr and the combination of EtBr and SG II were 0.048 and 0.007ng/μl, respectively. This highly sensitive DNA detection just using the combination of these dyes offering high resolution in a short time will be useful for various biological analyses.
National Institute of Advanced Industrial Science and Technology, Takamatsu 761-0395, Japan. yatsushiro.s@aist.go.jp
Shouki
S
Yatsushiro
Yuka
Y
Yamaguchi
Shohei
S
Yamamura
Yasuo
Y
Shinohara
Yoshinobu
Y
Baba
Masatoshi
M
Kataoka
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D053938:DNA Fragmentation; D046209:Electrophoresis, Microchip; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D057230:Limit of Detection; D008832:Microchemistry / Q000379:methods*; D008970:Molecular Weight; D009930:Organic Chemicals / Q000737:chemistry; D010957:Plasmids / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009930:Organic Chemicals; C098798:SYBR Green II; D004247:DNA; D004996:Ethidium