Buffers to suppress sodium dodecyl sulfate adsorption to polyethylene oxide for protein separation on capillary polymer electrophoresis.
Although polyethylene oxide (PEO) offers several advantages as a sieving polymer in SDS capillary polymer electrophoresis (SDS-CPE), solution properties of PEO cause deterioration in the electrophoresis because PEO in solution aggregates itself, degrades into smaller pieces, and forms polymer-micelle complexes with SDS. We examined protein separation on SDS-CPE with PEO as a sieving matrix in four individual buffer solutions: Tris-CHES, Tris-Gly, Tris-Tricine, and Tris-HCl buffers. The solution properties of PEO as a sieving matrix in those buffers were examined by dynamic light scattering (DLS) and by surface tension. Preferential SDS adsorption onto PEO disturbed protein-SDS complexation and impaired the protein separation efficiency. Substantial adsorption of SDS to PEO was particularly observed in Tris-Gly buffer. The Tris-CHES buffer prevented SDS from adsorbing onto the PEO. Only Tris-CHES buffer achieved separation of six proteins. This study demonstrated efficient protein separation on SDS-CPE with PEO.
SORST, Japan Science and Technology Agency, Ibaraki, Japan.
Keiko
K
Sumitomo
Koichi
K
Mayumi
Hiroyuki
H
Minamikawa
Mitsutoshi
M
Masuda
Toru
T
Asahi
Toshimi
T
Shimizu
Kohzo
K
Ito
Yoshinori
Y
Yamaguchi
D000327:Adsorption / Q000187:drug effects; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry
D002021:Buffers; D011108:Polymers; D011506:Proteins; D012996:Solutions; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols