Detection of doxorubicin-induced apoptosis of leukemic T-lymphocytes by laser tweezers Raman spectroscopy.
Laser tweezers Raman spectroscopy (LTRS) was used to acquire the Raman spectra of leukemic T lymphocytes exposed to the chemotherapy drug doxorubicin at different time points over 72 hours. Changes observed in the Raman spectra were dependent on drug exposure time and concentration. The sequence of spectral changes includes an intensity increase in lipid Raman peaks, followed by an intensity increase in DNA Raman peaks, and finally changes in DNA and protein (phenylalanine) Raman vibrations. These Raman signatures are consistent with vesicle formation, cell membrane blebbing, chromatin condensation, and the cytoplasm of dead cells during the different stages of drug-induced apoptosis. These results suggest the potential of LTRS as a real-time single cell tool for monitoring apoptosis, evaluating the efficacy of chemotherapeutic treatments, or pharmaceutical testing.
Tobias J
TJ
Moritz
Douglas S
DS
Taylor
Denise M
DM
Krol
John
J
Fritch
James W
JW
Chan
J Clin Oncol. 1999 Sep;17(9):2941-53
10561374
Biophys J. 2003 Jun;84(6):3968-81
12770902
Apoptosis. 2002 Dec;7(6):537-48
12370496
Appl Spectrosc. 2003 Nov;57(11):1363-7
14658149
Integr Biol (Camb). 2009 Jan;1(1):43-52
20023790
Leuk Res. 2000 May;24(5):445-52
10785267
Analyst. 2009 Jul;134(7):1440-6
19562213
Opt Lett. 2002 Feb 15;27(4):249-51
18007769
Br J Cancer. 1989 Aug;60(2):206-10
2765367
Appl Spectrosc. 2010 Nov;64(11):1308-10
21073802
Lab Chip. 2005 Apr;5(4):431-6
15791341
Biophys J. 2006 Jan 15;90(2):648-56
16239327
Anal Chem. 2008 Mar 15;80(6):2180-7
18260656
Curr Opin Oncol. 2009 Nov;21(6):582-8
19667983
J Cell Biochem. 2006 Sep 1;99(1):178-86
16598770
Curr Opin Chem Biol. 2009 Dec;13(5-6):556-61
19833543
J Biomed Opt. 2007 Jul-Aug;12(4):044009
17867813
J Immunol. 2002 Nov 1;169(9):4805-10
12391190
Exp Cell Res. 1991 Aug;195(2):361-7
2070819
Cancer. 1994 Apr 15;73(8):2013-26
8156506
Biochem J. 1996 Sep 15;318 ( Pt 3):749-52
8836114
Nat Cell Biol. 2001 Apr;3(4):339-45
11283606
Blood. 1999 May 1;93(9):3053-63
10216102
Anal Chem. 2004 Feb 1;76(3):599-603
14750852
Curr Treat Options Oncol. 2006 Mar;7(2):103-10
16455021
Exp Cell Res. 2000 Jul 10;258(1):223-35
10912804
J Biomed Opt. 2009 Nov-Dec;14(6):064013
20059251
Biochim Biophys Acta. 1997 Apr 17;1335(1-2):199-208
9133657
Carcinogenesis. 2000 Mar;21(3):485-95
10688869
Nat Rev Cancer. 2009 Jul;9(7):501-7
19550425
FEBS Lett. 1997 Nov 17;417(3):360-4
9409752
Br J Haematol. 2001 Oct;115(1):34-45
11722407
Opt Express. 2006 Jun 12;14(12):5779-91
19516747
Lab Chip. 2008 Jul;8(7):1116-20
18584087
Nat Genet. 2003 May;34(1):85-90
12704389
J Cell Sci. 2000 Sep;113 ( Pt 17):2941-53
10934034
Nat Rev Mol Cell Biol. 2008 Mar;9(3):231-41
18073771