[Rapid determination of ethanol in blood by capillary-gas chromatography].
OBJECTIVE
To develop a method for the rapid determination of ethanol in blood with capillary-GC.


METHODS
0.50 mL of whole blood sample was taken and added with 1.00 mL of dimethyl sulfoxide (DMSO), and 2 g of anhydrous sodium sulfate. The supernatant of the sample solution was directly injected into GC for analysis.


RESULTS
Ethanol was separated from other substances in the sample. The liner range of ethanol detected by the capillary-GC was 0.0-300.0 mg/100 mL, and the detection limit was 0.2 mg/100 mL. The RSD for standard solution determination was 1.36%. Satisfactory results were obtained for the determination of ethanol in whole blood samples, with recoveries ranging from 90.9% to 107.3% and a RSD of 1.98%. The combined uncertainty was 2.2%.


CONCLUSIONS
This is a rapid, sensitive and simple method for determination of ethanol in large quantities of samples. The method has shortened the duration of analysis cycle in comparison with the traditional headspace-GC, with a reduction from 20-30 min to less than 10 min.
Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Lin
L
Ren
Cheng-jun
CJ
Sun
Xiao-li
XL
Zou
Hong-yan
HY
Zeng
Bo
B
Jiang
D002849:Chromatography, Gas / Q000379:methods*; D000431:Ethanol / Q000097:blood*; D006801:Humans; D012680:Sensitivity and Specificity
D000431:Ethanol