Production and chemical characterization of an exopolysaccharide synthesized by psychrophilic yeast strain Sporobolomyces salmonicolor AL₁ isolated from Livingston Island, Antarctica.
The exopolysaccharide (EPS) production by psychrophilic Antarctic yeast Sporobolomyces salmonicolor AL₁ reached the maximum yield in medium containing sucrose (50 g/L) and diammonium sulfate (2.5 g/L) after a 5-d fermentation (5.64 g/L) at 22 °C, the dynamic viscosity of the culture broth reaching (after 5 d) 15.4 mPa s. EPS showed a mannan-like structure and high molar mass, and did not affect cellular viability and proliferation of murine macrophages. It exhibited also a protective effect against the toxic activity of Avarol.
Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, 80078 Pozzuoli, Italy. annarita.poli@icb.cnr.it
A
A
Poli
G
G
Anzelmo
G
G
Tommonaro
K
K
Pavlova
A
A
Casaburi
B
B
Nicolaus
D000818:Animals; D000864:Antarctic Regions; D001487:Basidiomycota / Q000302:isolation & purification / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D008264:Macrophages / Q000502:physiology; D008351:Mannans / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012717:Sesquiterpenes / Q000037:antagonists & inhibitors
D003470:Culture Media; D008351:Mannans; D011134:Polysaccharides; D012717:Sesquiterpenes; C023663:avarol
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