A comparative study of the effects of bromelain and fresh pineapple juice on the early phase of healing in acute crush achilles tendon injury.
Bromelain, an enzyme extracted from the stem of the pineapple plant, has been reported to reduce pain and swelling in acute soft tissue injuries, but no study has been done to compare its effect with that of fresh pineapple juice on the healing of acute tendon injuries. This study compared the effects of commercial bromelain and fresh pineapple juice on tenocyte proliferation and the malondialdehyde (MDA) level in the early stage of healing in a crush injury to the Achilles tendon of Sprague-Dawley rats. Twenty-four male rats were divided randomly into three groups of eight rats each; all the rats had induced crush injury to the Achilles tendon: Group 1 (control), no treatment; Group 2, oral bromelain treatment at a dosage of 7 mg/kg of body weight daily; and Group 3, fresh diluted pineapple juice at a dosage of 30 mg/kg of body weight. Treatment was given over the first 14 days post-injury. On day 15 post-injury, the animals were sacrificed, and the tendons were excised and processed for histological study and MDA assay. Results show a significant difference in the tenocyte population between the bromelain group and the control (P < .05), whereas pineapple juice also increased the tenocyte population, although not significantly (P = .36). Pineapple juice, however, significantly lowered the MDA level compared with both the control and bromelain-treated groups. Based on this study, 600 GDU bromelain given at a dosage of 7 mg/kg had a better effect on tenocyte proliferation than fresh pineapple juice given once daily in acute tendon injury.
Department of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria. bogphysio@yahoo.com
Ayoola I
AI
Aiyegbusi
Olaleye O
OO
Olabiyi
Francis I O
FI
Duru
Cressie C
CC
Noronha
Abayomi O
AO
Okanlawon
D000125:Achilles Tendon / Q000187:drug effects* / Q000293:injuries*; D000208:Acute Disease / Q000628:therapy; D000284:Administration, Oral; D031230:Ananas / Q000737:chemistry*; D000818:Animals; D001628:Beverages / Q000032:analysis; D001963:Bromelains / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005638:Fruit / Q000737:chemistry; D008297:Male; D010146:Pain / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013708:Tendon Injuries / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects
D010936:Plant Extracts; D001963:Bromelains