[Studies on separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin].
OBJECTIVE
To study the technology for separation and purification of phenylethanoid glycosides from leaves of Forsythia suspensa by macroporous adsorption resin.


METHODS
The absorption and separation abilities of 10 kinds of adsorption resin were studied and the separation and purification technological process of phenylethanoid glycosides from leaves of Forsythia suspensa was investigated by HPLC with the content of Forsythoside A as an index.


RESULTS
The optimal conditions were as follows: taking AB-8 resin column as adsorbent, the resin was washed by 20BV distilled water to remove impurity and 8BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2BV/h, the content of Forsythoside A in the extract was 34.8%.


CONCLUSIONS
The process with AB-8 resin is an effective method to separate and purify phenylethanoid glycosides.
Department of Pharmacy, Xi'an Medical College, Xi'an 710021, China. jinghui1204@sohu.com
Hui
H
Jing
Jiao-She
JS
Li
Yin-Jing
YJ
Yang
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D031657:Forsythia / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*; D010626:Phenylethyl Alcohol / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D012117:Resins, Synthetic; D012997:Solvents; D000431:Ethanol; D010626:Phenylethyl Alcohol; C058049:forsythiaside