Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells.
OBJECTIVE
The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro.


METHODS
Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method.


RESULTS
Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups.


CONCLUSIONS
Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.
Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.
Hak-Sung
HS
Kim
Kyoung-Hwa
KH
Kim
Su-Hwan
SH
Kim
Young-Sung
YS
Kim
Ki-Tae
KT
Koo
Tae-Il
TI
Kim
Yang-Jo
YJ
Seol
Young
Y
Ku
In-Chul
IC
Rhyu
Chong-Pyoung
CP
Chung
Yong-Moo
YM
Lee
J Endod. 2008 Feb;34(2):166-71
18215674
Biochem Biophys Res Commun. 2001 Oct 26;288(2):413-9
11606058
Transplant Proc. 2004 Dec;36(10):3272-5
15686744
J Exp Med. 2002 Aug 19;196(4):459-68
12186838
Stem Cells. 2006 Jan;24(1):74-85
16099998
J Periodontal Res. 2006 Aug;41(4):303-10
16827724
Tissue Eng Part B Rev. 2008 Mar;14(1):53-60
18454634
Nat Med. 2000 May;6(5):543-8
10802710
J Immunol Methods. 2006 May 30;312(1-2):126-36
16712866
J Cell Physiol. 2009 Jun;219(3):667-76
19160415
Exp Cell Res. 2006 Jul 15;312(12):2169-79
16631737
Annu Rev Immunol. 2002;20:795-823
11861618
PLoS One. 2006 Dec 20;1:e79
17183711
Blood. 2005 Mar 1;105(5):2214-9
15514012
Blood. 2007 Nov 15;110(10):3499-506
17664353
Lancet. 2004 Jul 10-16;364(9429):149-55
15246727
Periodontol 2000. 2009;51:239-51
19878478
Blood. 2002 May 15;99(10):3838-43
11986244
Transplantation. 2003 Oct 27;76(8):1208-13
14578755
Ann N Y Acad Sci. 2009 Sep;1176:101-17
19796238
Cell Immunol. 1994 Apr 1;154(1):296-308
8131204
Br J Haematol. 2005 Apr;129(1):118-29
15801964
Transplantation. 2003 Feb 15;75(3):389-97
12589164
Exp Hematol. 2003 Oct;31(10):890-6
14550804
J Periodontol. 2009 Nov;80(11):1815-23
19905951
Blood. 2003 Nov 15;102(10):3837-44
12881305
Bone. 1997 Sep;21(3):225-35
9276087