High-throughput FCS using an LCOS spatial light modulator and an 8 × 1 SPAD array.
We present a novel approach to high-throughput Fluorescence Correlation Spectroscopy (FCS) which enables us to obtain one order of magnitude improvement in acquisition time. Our approach utilizes a liquid crystal on silicon spatial light modulator to generate dynamically adjustable focal spots, and uses an eight-pixel monolithic single-photon avalanche photodiode array. We demonstrate the capabilities of this system by showing FCS of Rhodamine 6G under various viscosities, and by showing that, with proper calibration of each detection channel, one order of magnitude improvement in acquisition speed is obtained. More generally, our approach will allow higher throughput single-molecule studies to be performed.
Ryan A
RA
Colyer
Giuseppe
G
Scalia
Ivan
I
Rech
Angelo
A
Gulinatti
Massimo
M
Ghioni
Sergio
S
Cova
Shimon
S
Weiss
Xavier
X
Michalet
J Microsc. 2001 Nov;204(Pt 2):119-35
11737545
Science. 1999 Mar 12;283(5408):1676-83
10073925
J Biomed Opt. 2005 Sep-Oct;10(5):054008
16292968
Annu Rev Biophys Biomol Struct. 2007;36:151-69
17477838
Rev Sci Instrum. 2007 Apr;78(4):044102
17477680
Opt Express. 2005 Jan 24;13(2):352-7
19488360
Chem Rev. 2006 May;106(5):1785-813
16683755
Anal Chem. 2007 Jun 15;79(12):4463-70
17489557
Anal Chem. 2006 May 15;78(10):3444-51
16689548
Nat Struct Biol. 2000 Sep;7(9):724-9
10966638
Biophys Chem. 1990 Oct;38(1-2):49-57
2085652
Opt Express. 2010 May 10;18(10):10627-41
20588915
Methods. 2001 Sep;25(1):87-102
11559000
Proc SPIE Int Soc Opt Eng. 2010 Jan 23;7571:75710G-75710G11
21643532
Proc SPIE Int Soc Opt Eng. 2008 Feb 15;6862(68620F):
21822361
Biophys J. 2006 Dec 1;91(11):4241-52
16950838
Opt Lett. 1999 May 1;24(9):608-10
18073798
Opt Lett. 2006 Mar 15;31(6):829-31
16544638
Biophys J. 2003 Mar;84(3):2030-42
12609905
Biophys J. 2001 Jun;80(6):2987-99
11371471
Mol Cell. 2005 Nov 11;20(3):347-56
16285917
J Biomed Opt. 2004 Sep-Oct;9(5):913-21
15447011
Opt Express. 2000 Oct 9;7(8):273-9
19407875
Opt Lett. 1998;23(9):655-7
18087301
Opt Express. 2006 Jun 12;14(12):5013-20
19516661
Opt Express. 2005 Jul 25;13(15):5831-45
19498588