Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01.
Cell fractions including heat-treated cells, crude cell walls, intracellular extracts and exopolysaccharides (EPSs) obtained from Lactobacillus casei 01 were first studied for their effects on the proliferation of human intestinal epithelial cells, intestine 407 and the human colon cancer cell, HT-29. Their effects on the cytotoxicity of 4-nitroquinoline 1-oxide (4-NQO) against intestine 407 were further investigated. The results revealed that EPS exhibited the highest antiproliferation activity on HT-29 cells while the viability of intestine 407 cells was not affected by EPS at a concentration of 5-50μg/mL. It was also noted that all the cell fractions and EPS from L. casei 01 reduced the cytotoxicity of 4-NQO against intestine 407 with EPS showing the highest anticytotoxic activity. Additionally, it was found that EPS might exert blocking and bioanticytotoxic effects by both adjusting the function of intestine 407 and repairing the 4-NQO-damaged cells, thus reducing cytotoxicity of 4-NQO.
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
Chu-Ting
CT
Liu
Fang-Jung
FJ
Chu
Cheng-Chun
CC
Chou
Roch-Chui
RC
Yu
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007422:Intestines / Q000187:drug effects; D007780:Lacticaseibacillus casei / Q000737:chemistry* / Q000166:cytology; D009153:Mutagens / Q000633:toxicity*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*
D016587:Antimutagenic Agents; D009153:Mutagens; D011135:Polysaccharides, Bacterial; D015112:4-Nitroquinoline-1-oxide