Membrane lipidome of an epithelial cell line.
Tissue differentiation is an important process that involves major cellular membrane remodeling. We used Madin-Darby canine kidney cells as a model for epithelium formation and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa. To achieve this, we developed a shotgun-based lipidomics workflow that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts. Epithelial morphogenesis was accompanied by a major shift from sphingomyelin to glycosphingolipid, together with an increase in plasmalogen, phosphatidylethanolamine, and cholesterol content, whereas the opposite changes took place during an epithelial-to-mesenchymal transition. Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet.
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany. sampaio@mpi-cbg.de
Julio L
JL
Sampaio
Mathias J
MJ
Gerl
Christian
C
Klose
Christer S
CS
Ejsing
Hartmut
H
Beug
Kai
K
Simons
Andrej
A
Shevchenko
D000818:Animals; D002460:Cell Line; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D008563:Membrane Lipids / Q000378:metabolism*; D009024:Morphogenesis
D008563:Membrane Lipids
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