Sterilization and protection of protein in combinations of Camellia sinensis green tea extract and gamma irradiation.
Sterilization of milk protein without heating is of great interest. Gamma irradiation is a very powerful method to decontaminated casein. Gamma-irradiation of proteins in aqueous media at doses higher than 5kGy is known to induce their aggregation (without oxygen) or degradation (in presence of oxygen). Camellia sinensis green tea extract addition before irradiation of caseins cow milk proteins was examined. It was found that the presence of C. sinensis green tea extract during irradiation in the presence of oxygen conditions prevented the protein aggregation even at doses higher than 10kGy, probably by scavenging oxygen radicals produced by irradiation. The protective role of C. sinensis green tea extract allowing the gamma-irradiation treatment of caseins cow milk proteins in solution, was asserted by sodium dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and by high performance liquid chromatography inverse phase (RP-HPLC). The total viable microorganisms content evaluated by Plate Count Agar (PCA) incubation for 12h at 37°C, showed that caseins protein preparations gamma-irradiated remained sterile at a dose 2kGy in absence of C. sinensis green tea extract and at a dose lower than 2kGy in the presence of C. sinensis green tea extract.
Unité de Recherche de Biochimie des Lipides et Interactions Avec les Macromolécules, 03/UR/0902, Laboratoire de Biochimie et de Biologie Moléculaire, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia. kouasssahbanisaloua@rocketmail.com
Kouass Sahbani
KS
Saloua
Kouass
K
Salah
Benbettaieb
B
Nasreddine
Ayari
A
Samia
Saidi
S
Mouldi
Landoulsi
L
Ahmed
D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000528:radiation effects; D058491:Bacterial Load; D028241:Camellia sinensis / Q000737:chemistry*; D002364:Caseins / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D005511:Food Handling / Q000379:methods*; D005720:Gamma Rays; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D010936:Plant Extracts / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects / Q000528:radiation effects; D013242:Sterilization / Q000379:methods*
D002364:Caseins; D010936:Plant Extracts