The role of XPD in cell apoptosis and viability and its relationship with p53 and cdk2 in hepatoma cells.
We investigated the role of XPD in cell apoptosis of hepatoma and its relationship with p53 during the regulation of hepatoma bio-behavior. RT-PCR and Western blot were used to detect the expression levels of XPD, p53, c-myc, and cdk2. The cell apoptosis and cell cycle were analyzed with flow cytometry. Compared with the control cells, XPD-transfected cells displayed a lower viability and higher apoptosis rate. A decreased expression of p53 gene was detected in XPD-transfected cells. In contrast, both c-myc and cdk2 showed increased expressions of mRNAs and proteins in the transfected cells. Our results indicate that XPD may play an important role in cell apoptosis of hepatoma by inducing an over-expression of p53, but suppressing expressions of c-myc and cdk2.
Department of Gastroenterology, Second Affiliated Hospital, Nanchang University, 330006 Nanchang City, Jiangxi, People's Republic of China.
Hong-yun
HY
Wang
Gao-fei
GF
Xiong
Ji-xiang
JX
Zhang
Hong
H
Xu
Wu-hua
WH
Guo
Jiang-jing
JJ
Xu
Xiang-yang
XY
Xiong
D017209:Apoptosis / Q000502:physiology; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002469:Cell Separation; D002470:Cell Survival; D051357:Cyclin-Dependent Kinase 2 / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics / Q000378:metabolism*
C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human
J Virol. 1998 Jan;72(1):266-72
9420223
EMBO J. 1996 Apr 15;15(8):1877-84
8617234
Mol Cancer Res. 2005 Oct;3(10):531-9
16254187
J Hepatol. 2004 Nov;41(5):815-22
15519655
Oncogene. 1999 Aug 19;18(33):4681-8
10467415
Mol Cell Biol. 2000 Jul;20(13):4849-58
10848610
Mol Cell Biol. 2000 May;20(10):3705-14
10779360
Med Sci (Paris). 2006 Dec;22(12):1087-94
17156731
FEBS Lett. 2002 May 8;518(1-3):144-8
11997035
Mol Cell. 2000 Feb;5(2):331-41
10882074
Genes Dev. 1996 May 15;10(10):1219-32
8675009
EMBO J. 1996 Apr 1;15(7):1615-24
8612585
Proteins. 2007 Jun 1;67(4):1048-59
17373709
DNA Repair (Amst). 2007 Sep 1;6(9):1264-70
17403617
Biochimie. 2003 Nov;85(11):1101-11
14726016
Nat Genet. 1998 Oct;20(2):184-8
9771713
J Epidemiol. 2002 May;12(3):229-36
12164325
J Biol Chem. 2001 Apr 27;276(17):14124-32
11278765
J Dtsch Dermatol Ges. 2006 Sep;4(9):721-31
16928240
Cancer Res. 2001 Apr 15;61(8):3245-9
11309273
Neonatal Netw. 2003 May-Jun;22(3):5-15
12795504
Int J Cancer. 1999 Mar 15;80(6):875-9
10074921
Acta Oncol. 2001;40(6):696-701
11765063
Cell. 2001 Feb 9;104(3):353-63
11239393
Nature. 2003 Jul 10;424(6945):228-32
12853965
Mol Cell Biol. 2005 Jan;25(1):147-61
15601838
J Biol Chem. 2004 Nov 5;279(45):46674-7
15342631
Nature. 1993 Oct 28;365(6449):852-5
8413672