Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells.
BACKGROUND
Cytotoxic cells of the immune system have evolved a lysosomal compartment to store and mobilize effector molecules. In T lymphocytes and NK cells, the death factor FasL is one of the characteristic marker proteins of these so-called secretory lysosomes, which combine properties of conventional lysosomes and exocytotic vesicles. Although these vesicles are crucial for immune effector function, their protein content in T cells has so far not been investigated in detail.


RESULTS
In the present study, intact membranous vesicles were enriched from homogenates of polyclonally activated T cells and initially characterized by Western blotting and electron microscopic inspection. The vesicular fraction that contained the marker proteins of secretory lysosomes was subsequently analyzed by 2D electrophoresis and mass spectrometry. The proteome analysis and data evaluation revealed that 70% of the 397 annotated proteins had been associated with different lysosome-related organelles in previous proteome studies.


CONCLUSIONS
We provide the first comprehensive proteome map of T cell-derived secretory lysosomes with only minor contaminations by cytosolic, nuclear or other proteins. This information will be useful to more precisely address the activation-dependent maturation and the specific distribution of effector organelles and proteins in individual T or NK cell populations in future studies.
Institute of Immunology, Christian-Albrechts-University, UK S-H Campus Kiel, Kiel, Germany. ojanssen@email.uni-kiel.de.
Hendrik
H
Schmidt
Christoph
C
Gelhaus
Melanie
M
Nebendahl
Marcus
M
Lettau
Ralph
R
Lucius
Matthias
M
Leippe
Dietrich
D
Kabelitz
Ottmar
O
Janssen
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