High-speed synthetic aperture microscopy for live cell imaging.
We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.
Department of Physics, Korea University, Seoul 136-701, South Korea.
Moonseok
M
Kim
Youngwoon
Y
Choi
Christopher
C
Fang-Yen
Yongjin
Y
Sung
Ramachandra R
RR
Dasari
Michael S
MS
Feld
Wonshik
W
Choi
D002470:Cell Survival; D002784:Cholesterol / Q000378:metabolism; D008027:Light; D017628:Microglia / Q000166:cytology; D008853:Microscopy / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*
D002784:Cholesterol
Opt Lett. 2003 Aug 15;28(16):1424-6
12943079
Appl Opt. 2007 Feb 20;46(6):993-1000
17279147
Opt Lett. 2007 Jun 1;32(11):1572-4
17546192
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15663-6
19717442
Opt Express. 2009 Jul 20;17(15):12407-17
19654642
Opt Express. 2005 Nov 14;13(23):9361-73
19503137
Nat Methods. 2007 Sep;4(9):717-9
17694065
Opt Lett. 2010 Apr 15;35(8):1136-8
20410944