A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry.
Paper mulberry (Broussonetia papyrifera, syn. Morus papyrifera L.) is a Chinese traditional medicine and its low-molecular-weight extracts are reported to have antifungal activity. In this study, two proteins (PMAPI and PMAPII) with activity against Trichoderma viride were obtained from paper mulberry leaves with a fast protein liquid chromatography (FPLC) unit. The purification protocol employed (NH(4))(2)SO(4) precipitation, ion-exchange chromatography and hydrophobic-interaction chromatography on FPLC. Molecular masses were 18,798 Da for PMAPI, and 31,178 Da for PMAPII determined by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Peptide mapping fingerprint analysis showed that PMAPI has no peptides similar to PMAPII. N-terminal amino acid sequencing revealed that PMAPI is a hevein-like protein, and PMAPII is a class I chitinase. They both had a half-maximal inhibitory concentration (IC50) of 0.1 µg/µL against T. viride. This is the first report of high-molecular-weight extracts with antifungal activity from paper mulberry.
College of Pu-erh Tea, Yunnan Agricultural University, Kunming 650201, China.
Ming
M
Zhao
Yan
Y
Ma
Ying-Hong
YH
Pan
Chun-Hua
CH
Zhang
Wen-Xia
WX
Yuan
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D004365:Drugs, Chinese Herbal; D004591:Electrophoresis, Polyacrylamide Gel; D003904:Mitosporic Fungi / Q000187:drug effects; D008969:Molecular Sequence Data; D008970:Molecular Weight; D031625:Morus / Q000737:chemistry* / Q000201:enzymology; D010449:Peptide Mapping; D018515:Plant Leaves / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D004365:Drugs, Chinese Herbal; D037121:Plant Lectins; D010940:Plant Proteins; C045875:hevein; D002688:Chitinases; C119825:chitinase C