Microbial diagnostic microarray for food- and water-borne pathogens.
A microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogenetically robust gyrB marker gene allowed a highly specific (resolution on genus/species level) and sensitive (0.1% relative and 10(4) cfu absolute detection sensitivity) detection of the target pathogens. Validation was performed using a set of reference strains and a set of spiked environmental samples. Reliability of the obtained data was additionally verified by independent analysis of the samples via fluorescence in situ hybridization (FISH) and conventional microbiological reference methods. The applicability of this diagnostic system for food analysis was demonstrated through extensive validation using artificially and naturally contaminated spiked food samples. The microarray-based pathogen detection was compared with the corresponding microbiological reference methods (performed according to the ISO norm). Microarray results revealed high consistency with the reference microbiological data.
AIT Austrian Institute of Technology GmbH, Bioresources Unit, A-2444 Seibersdorf, Austria. tanja.kostic@ait.ac.at
Tanja
T
Kostić
Beatrix
B
Stessl
Martin
M
Wagner
Angela
A
Sessitsch
Levente
L
Bodrossy
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D027081:DNA Gyrase / Q000235:genetics; D005516:Food Microbiology; D046228:Microarray Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D012680:Sensitivity and Specificity; D014871:Water Microbiology
D015345:Oligonucleotide Probes; D027081:DNA Gyrase
Nature. 1947 Sep 20;160(4064):389
20263978
J Microbiol Methods. 2005 Apr;61(1):87-94
15676199
Nucleic Acids Res. 2004 Feb 25;32(4):1363-71
14985472
Int J Antimicrob Agents. 2008 May;31(5):440-51
18243668
ScientificWorldJournal. 2003 Jul 01;3:578-84
12847294
Sci Total Environ. 2008 Jul 15;398(1-3):203-11
18423816
Anal Biochem. 2007 Jan 15;360(2):244-54
17123456
J Clin Microbiol. 2007 Sep;45(9):2965-70
17596360
Biosens Bioelectron. 2004 Nov 1;20(4):684-98
15522583
Environ Microbiol. 2003 Jul;5(7):566-82
12823189
Lett Appl Microbiol. 2009 Sep;49(3):324-31
19552771
Environ Microbiol. 2004 Apr;6(4):347-63
15008813
Appl Environ Microbiol. 1995 Mar;61(3):1104-9
7793912
J Microbiol Methods. 2008 Dec;75(3):566-71
18834908
Environ Microbiol. 2004 Jul;6(7):754-9
15186354
Appl Environ Microbiol. 2007 Sep;73(17):5692-7
17630299
Appl Environ Microbiol. 2005 Dec;71(12):8548-57
16332846
Curr Opin Microbiol. 2003 Jun;6(3):288-94
12831906
Can J Microbiol. 2006 Jan;52(1):1-8
16541153
Int J Food Microbiol. 2001 Jul 20;67(1-2):71-80
11482571
Nucleic Acids Res. 2003 Jan 1;31(1):514-6
12520066
Environ Microbiol. 2003 Oct;5(10):933-43
14510847
J Microbiol Methods. 2003 May;53(2):235-43
12654494
Appl Environ Microbiol. 2005 Jul;71(7):3624-32
16000770
Biotechniques. 2004 Apr;36(4):664-70, 672, 674-5
15088384
Environ Microbiol. 2006 Feb;8(2):200-13
16423009
J Clin Microbiol. 2009 Jul;47(7):2347-8
19494066
Biochemistry. 1996 Mar 19;35(11):3555-62
8639506
Biosens Bioelectron. 2004 Nov 1;20(4):668-83
15522582
Crit Rev Microbiol. 2004;30(3):145-72
15490968