In vitro adhesion of staphylococci to diamond-like carbon polymer hybrids under dynamic flow conditions.
This study compares the ability of selected materials to inhibit adhesion of two bacterial strains commonly implicated in implant-related infections. These two strains are Staphylococcus aureus (S-15981) and Staphylococcus epidermidis (ATCC 35984). In experiments we tested six different materials, three conventional implant metals: titanium, tantalum and chromium, and three diamond-like carbon (DLC) coatings: DLC, DLC-polydimethylsiloxane hybrid (DLC-PDMS-h) and DLC-polytetrafluoroethylene hybrid (DLC-PTFE-h) coatings. DLC coating represents extremely hard material whereas DLC hybrids represent novel nanocomposite coatings. The two DLC polymer hybrid films were chosen for testing due to their hardness, corrosion resistance and extremely good non-stick (hydrophobic and oleophobic) properties. Bacterial adhesion assay tests were performed under dynamic flow conditions by using parallel plate flow chambers (PPFC). The results show that adhesion of S. aureus to DLC-PTFE-h and to tantalum was significantly (P < 0.05) lower than to DLC-PDMS-h (0.671 ± 0.001 × 10(7)/cm(2) and 0.751 ± 0.002 × 10(7)/cm(2) vs. 1.055 ± 0.002 × 10(7)/cm(2), respectively). No significant differences were detected between other tested materials. Hence DLC-PTFE-h coating showed as low susceptibility to S. aureus adhesion as all the tested conventional implant metals. The adherence of S. epidermidis to biomaterials was not significantly (P < 0.05) different between the materials tested. This suggests that DLC-PTFE-h films could be used as a biomaterial coating without increasing the risk of implant-related infections.
ORTON Research Institute of the ORTON Orthopaedic Hospital, Tenholantie 10, 00280, Helsinki, Finland. antti.soininen@orton.fi
Antti
A
Soininen
Jaakko
J
Levon
Maria
M
Katsikogianni
Katja
K
Myllymaa
Reijo
R
Lappalainen
Yrjö T
YT
Konttinen
Teemu J
TJ
Kinnari
Veli-Matti
VM
Tiainen
Yannis
Y
Missirlis
D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D002857:Chromium / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D066298:In Vitro Techniques; D018613:Microscopy, Confocal / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000378:metabolism; D013499:Surface Properties; D013635:Tantalum / Q000737:chemistry; D014025:Titanium / Q000737:chemistry
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D011108:Polymers; D002857:Chromium; C013830:baysilon; D013635:Tantalum; D002244:Carbon; D014025:Titanium
J Ind Microbiol Biotechnol. 2007 Sep;34(9):577-88
17619090
N Engl J Med. 2004 Oct 14;351(16):1645-54
15483283
N Engl J Med. 1997 Apr 3;336(14):999-1007
9077380
Clin Orthop Relat Res. 1998 Jul;(352):118-27
9678039
Colloids Surf B Biointerfaces. 2008 Sep 1;65(2):257-68
18565741
Res Microbiol. 2005 May;156(4):506-14
15862449
J Biomed Mater Res A. 2008 Sep;86(3):760-8
18041722
J Biomed Mater Res B Appl Biomater. 2006 Aug;78(2):230-6
16615067
N Engl J Med. 1998 Aug 20;339(8):520-32
9709046
J Biomed Mater Res. 1998 Fall;43(3):338-48
9730073
Biomaterials. 1991 Jan;12(1):37-40
2009344
Acc Chem Res. 2002 Jul;35(7):491-9
12118988
J Biomed Mater Res B Appl Biomater. 2003 Jul 15;66(1):410-3
12808601
J Biomed Mater Res A. 2010 Mar 15;92(4):1606-13
19437436
Appl Environ Microbiol. 1987 Aug;53(8):1898-901
3662520
J Biomed Mater Res. 2001 May 1;58(3):319-28
11319748
Colloids Surf B Biointerfaces. 2005 Nov 25;46(1):1-6
16198548
Thromb Haemost. 1986 Jun 30;55(3):415-35
3750272
Clin Infect Dis. 2001 Oct 15;33(8):1387-92
11565080
Ann Biomed Eng. 2001 Nov;29(11):956-62
11791678
Mol Microbiol. 2003 May;48(4):1075-87
12753197
Otolaryngol Clin North Am. 1995 Apr;28(2):225-34
7596603
J Hosp Infect. 1991 Jun;18 Suppl A:367-75
1679802
J Mater Sci Mater Med. 2006 Aug;17(8):679-89
16897160
J Ind Microbiol Biotechnol. 2008 Jul;35(7):751-60
18379832
J Mater Sci Mater Med. 1998 Jan;9(1):17-22
15348697
J Biomed Mater Res B Appl Biomater. 2009 Aug;90(2):882-5
19353566
Clin Infect Dis. 2001 Nov 1;33(9):1567-72
11577378
Lab Chip. 2003 May;3(2):67-72
15100784