Tanshinone IIA ameliorates seawater exposure-induced lung injury by inhibiting aquaporins (AQP) 1 and AQP5 expression in lung.
Aquaporins (AQPs), a family of transmembrane water channels, mediate physiological response to changes of fluid volume and osmolarity. It is still unknown what role of AQPs plays in seawater drowning-induced acute lung injury (ALI) and whether pharmacologic modulation of AQPs could alleviate the severity of ALI caused by seawater aspiration. In our study, the results from RT-PCR and Western blotting showed that intratracheal installation of seawater up-regulated the mRNA and protein levels of AQP1 and AQP5 in lung tissues. Furthermore, we found that treatment of tanshinone IIA (TIIA, one of the main active components from Chinese herb Danshen) significantly reduced the elevation of AQP1 and AQP5 expression induced by seawater in rats, A549 cells and primary alveolar type II cells. Treatment of TIIA also improved lung histopathologic changes and blood-gas indices, and reduced lung edema and vascular leakage. These findings demonstrated that AQP1 and AQP5 might play an important role in the development of lung edema and lung injury, and that treatment with TIIA could significantly alleviate seawater exposure-induced ALI, which was probably through the inhibition of AQP1 and AQP5 over-expression in lungs.
Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xinsi Road, Xi'an 710038, PR China.
Jiahuan
J
Li
Min
M
Xu
Qixin
Q
Fan
Xiaoyan
X
Xie
Yong
Y
Zhang
Deguang
D
Mu
Pengtao
P
Zhao
Bo
B
Zhang
Fale
F
Cao
Yanxia
Y
Wang
Faguang
F
Jin
Zhichao
Z
Li
D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D051398:Aquaporin 1 / Q000096:biosynthesis*; D051402:Aquaporin 5 / Q000096:biosynthesis*; D015153:Blotting, Western; D002460:Cell Line; D004195:Disease Models, Animal; D004332:Drowning / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012623:Seawater / Q000009:adverse effects*
D045784:Abietanes; D051402:Aquaporin 5; C021751:tanshinone; D051398:Aquaporin 1