Protein and peptide fractionation, enrichment and depletion: tools for the complex proteome.
The identification, quantitation and global characterisation of all proteins within a given proteome are extremely challenging. This is due to the absolute detection limits of technology as well as the dynamic range in expression of proteins; and the extreme diversity and heterogeneity of the proteome. To overcome such issues, the use of separation technologies has played a critical role in reducing sample complexity. To date, a plethora of chromatographic and electrophoretic fractionation tools have evolved over the years assisting in simplifying complex protein and peptide mixtures. Here, we review a range of these technologies highlighting the challenges of protein and peptide analysis in the context of proteome research and some of the advantages and disadvantages of present techniques.
Central Clinical School, Department of Neurology, University of Sydney, Sydney, NSW, Australia. linda.ly@sydney.edu.au
Linda
L
Ly
Valerie C
VC
Wasinger
D005591:Chemical Fractionation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002853:Chromatography, Liquid; D004586:Electrophoresis; D011506:Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
D011506:Proteins