Cannabidiol reduces lipopolysaccharide-induced vascular changes and inflammation in the mouse brain: an intravital microscopy study.
BACKGROUND
The phytocannabinoid cannabidiol (CBD) exhibits antioxidant and antiinflammatory properties. The present study was designed to explore its effects in a mouse model of sepsis-related encephalitis by intravenous administration of lipopolysaccharide (LPS).


METHODS
Vascular responses of pial vessels were analyzed by intravital microscopy and inflammatory parameters measured by qRT-PCR.


RESULTS
CBD prevented LPS-induced arteriolar and venular vasodilation as well as leukocyte margination. In addition, CBD abolished LPS-induced increases in tumor necrosis factor-alpha and cyclooxygenase-2 expression as measured by quantitative real time PCR. The expression of the inducible-nitric oxide synthase was also reduced by CBD. Finally, preservation of Blood Brain Barrier integrity was also associated to the treatment with CBD.


CONCLUSIONS
These data highlight the antiinflammatory and vascular-stabilizing effects of CBD in endotoxic shock and suggest a possible beneficial effect of this natural cannabinoid.
Laboratorio de Apoyo a la Investigación, Hospital Universitario Fundación Alcorcón and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativa, Alcorcón, 28922, Madrid, Spain.
Lourdes
L
Ruiz-Valdepeñas
José A
JA
Martínez-Orgado
Cristina
C
Benito
Africa
A
Millán
Rosa M
RM
Tolón
Julián
J
Romero
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects* / Q000473:pathology*; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology; D001921:Brain; D002185:Cannabidiol / Q000494:pharmacology / Q000627:therapeutic use; D002560:Cerebrovascular Circulation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D007962:Leukocytes / Q000166:cytology / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008853:Microscopy / Q000379:methods; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010841:Pia Mater / Q000098:blood supply*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D002185:Cannabidiol; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
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