Adhesion of preosteoblasts and fibroblasts onto poly(pentafluorostyrene)-based glycopolymeric films and their biocompatibility.
An efficient and metal-catalyst free method of glycopolymer synthesis via thiol/para-fluorine "click" reaction was used to graft acetylated 1-thio-β-D-glucopyranose and 1-thio-β-D-galactopyranose onto a homopolymer of pentafluorostyrene (PFS) as well as onto a block copolymer of styrene and PFS. Subsequent deprotection of the carbohydrate moieties yielded well-defined, sugar-modified polymers (PDI < 1.2). The prepared polymers were not cytotoxic against 3T3 fibroblasts and MC3T3-E1 preosteoblasts. Furthermore, the water-insoluble copolymers were drop-cast and examined as synthetic biocompatible coatings on poly(propylene) substrates for culturing the investigated cell types. Both fibro- and preosteoblasts showed stable adhesion and proliferation on the glycopolymer-coated surfaces.
Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, 07743 Jena, Germany.
Krzysztof
K
Babiuch
C Remzi
CR
Becer
Michael
M
Gottschaldt
Joseph T
JT
Delaney
Jürgen
J
Weisser
Birgitt
B
Beer
Ralf
R
Wyrwa
Matthias
M
Schnabelrauch
Ulrich S
US
Schubert
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D005465:Fluorocarbon Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D051379:Mice; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D005465:Fluorocarbon Polymers; D011137:Polystyrenes