Simplification of a complex microbial antilisterial consortium to evaluate the contribution of its flora in uncooked pressed cheese.
A complex microbial consortium derived from raw milk and composed of populations classified in 4 groups (lactic acid bacteria (A), Gram positive catalase positive bacteria (B), Gram negative bacteria (C) and yeasts (D)) can contribute to the inhibition of Listeria monocytogenes in the core of an uncooked pressed cheese. To identify what groups may be involved in the inhibition, the consortium was simplified by successively omitting one group at a time. Pasteurized milk was inoculated with these more or less complex consortia and their effects on L. monocytogenes count, pH, acids and volatile compounds in the core of uncooked pressed cheese were evaluated. The growth of L. monocytogenes was the highest in cheeses prepared with pasteurized milk and only St. thermophilus. Inhibition in other cheeses was expressed by comparison with growth in these ones. All the consortia containing both lactic acid bacteria (group A) and Gram positive catalase positive bacteria (group B)--ABCD, ABD, ABC, AB--were more inhibitory than those containing lactic acid bacteria on its own (A) or associated only with yeasts (AD) or/and Gram negative (ADC). Consortia without lactic acid bacteria were weakly inhibitory or had no effect. Gram positive catalase positive bacteria alone were not inhibitory although most of the species became established in the cheeses. The Lactobacillus population (Lb. casei, Lb. plantarum, Lb. curvatus and Lb. farciminis) was predominant in cheeses (9 log CFU/g) with a higher count than Leuconostoc (7 log CFU/g) and Enterococcus (7 log CFU/g). Lactobacillus counts were negatively correlated with those of L. monocytogenes (r=-0.84 at 18 days) and with the level of D-lactic acid. There was no correlation between L. monocytogenes and Leuconostoc or Enterococcus counts. Complex consortium ABCD and AB not only had a stronger inhibitory power in cheeses than consortium AD, they were also associated with the highest levels of L-lactic and acetic acids. All cheeses inoculated with lactic acid bacteria differed from those without by higher levels of ethyl formiate, pentane and alcohols (2-butanol, 2-pentanol), and lower levels of ketones (2-hexanone, 2,3-butanedione) and aldehydes (2-methyl-butanal). Levels of 2-methyl-butanal, 2-butanol and 2-pentanol were higher in ABCD and AB cheeses than in AD cheeses. Beside their contribution to the inhibition, their effect on cheese flavour must be evaluated.
INRA, UR 545 Fromagères, 20, Côte de Reyne, F-15000 Aurillac, France. callon@clermont.inra.fr
Cécile
C
Callon
Marjorie
M
Saubusse
Robert
R
Didienne
Solange
S
Buchin
Marie-Christine
MC
Montel
D019342:Acetic Acid / Q000032:analysis; D000438:Alcohols / Q000032:analysis; D000818:Animals; D000898:Antibiosis; D002611:Cheese / Q000032:analysis / Q000382:microbiology*; D015169:Colony Count, Microbial; D005227:Fatty Acids / Q000032:analysis; D006094:Gram-Positive Bacteria / Q000254:growth & development; D007778:Lactobacillus / Q000254:growth & development; D008089:Listeria monocytogenes / Q000254:growth & development*; D059013:Microbial Consortia; D008892:Milk / Q000382:microbiology; D013696:Temperature; D055549:Volatile Organic Compounds / Q000032:analysis; D015003:Yeasts / Q000254:growth & development
D000438:Alcohols; D005227:Fatty Acids; D055549:Volatile Organic Compounds; D019342:Acetic Acid