Flavonol-rich fractions of yaupon holly leaves (Ilex vomitoria, Aquifoliaceae) induce microRNA-146a and have anti-inflammatory and chemopreventive effects in intestinal myofibroblast CCD-18Co cells.
Polyphenolics extracted from yaupon holly (Ilex vomitoria, Aquifoliaceae) (YH) leaves were investigated in human colon cells for their chemopreventive and anti-inflammatory activities. An activity-guided fractionation allowed the selection of YH flavonol-rich fraction due to its preferential inhibition of HT-29 colon cancer viability over the normal CCD-18Co colon cells. Quercetin and kaempferol 3-rutinosides, main components identified in this fraction, protected CCD-18Co cells against reactive oxidative species (ROS) in part due to increased activity of antioxidant enzymes. In addition, up-regulation of microRNA-146a (miR-146a) known as a negative regulator of pro-inflammatory NF-κB activation was the underlying molecular mechanism that protected CCD-18Co from inflammation.
Department of Nutrition and Food Science, Texas A&M University, College Station, TX 77843, USA.
Giuliana D
GD
Noratto
Youngmok
Y
Kim
Stephen T
ST
Talcott
Susanne U
SU
Mertens-Talcott
D000893:Anti-Inflammatory Agents / Q000032:analysis*; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis; D001711:Biotransformation / Q000187:drug effects; D002470:Cell Survival; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000032:analysis*; D019073:HT29 Cells; D006801:Humans; D030020:Ilex vomitoria / Q000737:chemistry*; D008070:Lipopolysaccharides; D035683:MicroRNAs / Q000378:metabolism*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols; D014157:Transcription Factors / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005419:Flavonoids; D008070:Lipopolysaccharides; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D014157:Transcription Factors