Evaluation of carbamate insecticides as chemotherapeutic agents for cancer.
BACKGROUND
Cancer chemotherapy has already been in practice by the use of toxins and some of the specific poisonous compounds of cyanide derivatives. Carbamate insecticides inhibit cellular metabolism including energy, protein, and nucleic acid metabolism, thereby, causing cell regression and death.


OBJECTIVE
Preliminary evaluation of three carbamate insecticides, namely, baygon, carbaryl, and carbofuran as chemotherapeutic agents for cancer is undertaken in the present study.


METHODS
The toxicity of carbamates on squamous cell carcinoma was assessed in-vitro using dye binding tests. Cells were grown in microtitration ELISA plates, as adherent cultures, for six hours, and then exposed to the drugs for 2, 4, 8, and 12 hours, and finally stained with neutral red, to assess the viable cell number, and with methylene blue for the determination of protein in the monolayer. Optical density was read in an ELISA reader.


METHODS
The data obtained during the experiment was subjected to statistical analysis by using the student 't' test.


RESULTS
The results indicated that the percentage of the viable cell number reduced with an increase in the time of exposure of the drugs. Exposure of the tumor cells to the drugs for 12 hours detached them completely from the wells, and hence, all the cells were washed out. Exposure of the drugs prior to the establishment of the culture in-vitro resulted in the non-formation of the monolayer in the wells.


CONCLUSIONS
Among the three drugs studied, the survival percent was least with carbaryl treatment followed by baygon, and with carbofuran treatment it was almost near to control group.
Department of Biochemistry, College of Medicine, King Khalid University, Abha, Saudi Arabia. amanullahmohammed@yahoo.com
Mohd
M
Amanullah
Babu Y
BY
Hari
D000818:Animals; D002219:Carbamates / Q000633:toxicity*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D002417:Cattle; D006258:Head and Neck Neoplasms / Q000188:drug therapy*; D007306:Insecticides / Q000633:toxicity*; D008297:Male
D002219:Carbamates; D007306:Insecticides