A processed noncoding RNA regulates an altruistic bacterial antiviral system.
The ≥ 10³⁰ bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanisms for this toxicity and inhibition have not been defined. Here we present the crystal structure of the ToxN-ToxI complex from Pectobacterium atrosepticum, determined to 2.75-Å resolution. ToxI is a 36-nucleotide noncoding RNA pseudoknot, and three ToxI monomers bind to three ToxN monomers to generate a trimeric ToxN-ToxI complex. Assembly of this complex is mediated entirely through extensive RNA-protein interactions. Furthermore, a 2'-3' cyclic phosphate at the 3' end of ToxI, and catalytic residues, identify ToxN as an endoRNase that processes ToxI from a repetitive precursor but is regulated by its own catalytic product.
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Tim R
TR
Blower
Xue Y
XY
Pei
Francesca L
FL
Short
Peter C
PC
Fineran
David P
DP
Humphreys
Ben F
BF
Luisi
George P C
GP
Salmond
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001435:Bacteriophages / Q000502:physiology; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D044043:Pectobacterium / Q000737:chemistry / Q000235:genetics* / Q000502:physiology* / Q000821:virology; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D022661:RNA, Untranslated / Q000737:chemistry* / Q000378:metabolism*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D022661:RNA, Untranslated; D004722:Endoribonucleases
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