Interactions of leukocytes and platelets with poly(lysine/leucine) immobilized on tetraethylene glycol-terminated self-assembled monolayers.
Surfaces that bind heparin are important for biomaterials for blood deheparinization. In our recent work it was demonstrated that a polypeptide composed of L-lysine and L-leucine (pKL), after immobilization onto tetra(ethylene glycol) terminated self-assembled monolayers (EG4-SAMs), can bind heparin from blood plasma in a selective, concentration-dependent way. During this work the effect of this peptide on platelet adhesion and activation and leukocyte adhesion was studied. The surface charge of these nanostructured surfaces was evaluated in order to correlate the effect of positively charged amine groups and hydrophobic methyl groups on the behavior of platelets and leukocyte adhesion. The results demonstrated that the presence of pKL decreased leukocyte adhesion to EG4-SAMs at all concentrations used. This effect is even more pronounced when surfaces were pre-immersed in heparinized plasma. In contrast, there is an increase in platelet adhesion and activation with increased percentage immobilized pKL. This effect is enhanced when surfaces were pre-immersed in heparinized plasma. However, adsorbed pKL in very low amounts does not induce platelet adhesion and activation compared with EG4, even when pre-immersed in plasma. Since only low pKL amounts are necessary to induce heparin selectivity, these results are promising for the development of heparin-binding biomaterials for blood deheparinization.
Instituto de Engenharia Biomédica, Divisão de Biomateriais, Universidade do Porto, Rua do Campo Alegre, Portugal. cmartins@ineb.up.pt
M Cristina L
MC
Martins
Vanessa
V
Ochoa-Mendes
Gisela
G
Ferreira
Judite N
JN
Barbosa
Scott A
SA
Curtin
Buddy D
BD
Ratner
Mário A
MA
Barbosa
D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D002450:Cell Communication / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D019855:Ethylene Glycol / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D055767:Immobilized Proteins / Q000494:pharmacology*; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D010973:Platelet Adhesiveness / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D055672:Static Electricity; D013499:Surface Properties / Q000187:drug effects
D007093:Imidazoles; D055767:Immobilized Proteins; D011506:Proteins; D011107:Polylysine; D011092:Polyethylene Glycols; C006900:N,N-carbonyldiimidazole; D019855:Ethylene Glycol