Disposition of cannabichromene, cannabidiol, and Δ⁹-tetrahydrocannabinol and its metabolites in mouse brain following marijuana inhalation determined by high-performance liquid chromatography-tandem mass spectrometry.
A liquid chromatography-tandem mass spectrometry (LC-MS-MS)method was developed for the analysis of marijuana cannabinoids in mouse brain tissue using an Applied Biosystems 3200 Q trap with a turbo V source for TurbolonSpray attached to a Shimadzu SCL HPLC system. The method included cannabichromene (CBC),cannabidiol (CBD), Δ⁹-tetrahydrocannabinol (THC), 11-hydroxytetrahydrocannabinol (11-OH-THC), and 11-nor-Δ⁹-tetrahydrocannabinol-9-carboxylic acid (THC-COOH). These compounds were isolated by liquid-liquid extraction using cold acetonitrile. The following transition ions were monitored by multiple reaction monitoring (MRM): m/z 315>193, 315>259 for THC/CBD/CBC; m/z 331>193, 331>105 for 11-OH-THC; m/z 345>299, 345>193 for THC-COOH; m/z 318>196 for THC-d₃; m/z 334>196 for 11-OH-THC-d₃, and m/z 348>302 for THC-COOH-d₃. Linearity for THC, 1-OH-THC, and THC-COOH was 1-200 ng/g; for CBC and CBD, it was 0.5-20 ng/g. Within-run and between-run precisions for all the analytes yielded coefficients of variation of < 20%. Four C57BL6 mice were sacrificed 20 min after nose-only exposure to the smoke of 200 mg of marijuana containing 0.44 mg CBC, 0.93 mg CBD, and 8.81 mg THC. The mean brain concentrations were 3.9 ± 1.5 ng/g CBC, 21 ± 3.9 ng/g CBD, 364 ± 74 ng/g THC, and 28 ± 5.9 ng/g 11-OH-THC. THC-COOH was not detected. The relative mean brain cannabinoid concentrations correlated to the amounts of the cannabinoids in the inhaled marijuana.
Department of Pharmacology, Virginia Commonwealth University, Richmond, Virginia, USA.
Justin L
JL
Poklis
Candace C
CC
Thompson
Kelly A
KA
Long
Aron H
AH
Lichtman
Alphonse
A
Poklis
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002185:Cannabidiol / Q000032:analysis / Q000378:metabolism*; D002186:Cannabinoids / Q000032:analysis / Q000378:metabolism*; D002188:Cannabis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000032:analysis / Q000378:metabolism*; D019570:Inhalation Exposure; D057230:Limit of Detection; D008297:Male; D008385:Marijuana Smoking / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution
D002186:Cannabinoids; D010936:Plant Extracts; D002185:Cannabidiol; D013759:Dronabinol; C010695:cannabichromene
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