Hoxb8 conditionally immortalised macrophage lines model inflammatory monocytic cells with important similarity to dendritic cells.
We have examined the potential to generate bona fide macrophages (MØ) from conditionally immortalised murine bone marrow precursors. MØ can be derived from Hoxb8 conditionally immortalised macrophage precursor cell lines (MØP) using either M-CSF or GM-CSF. When differentiated in GM-CSF (GM-MØP) the resultant cells resemble GM-CSF bone marrow-derived dendritic cells (BMDC) in morphological phenotype, antigen phenotype and functional responses to microbial stimuli. In spite of this high similarity between the two cell types and the ability of GM-MØP to effectively present antigen to a T-cell hybridoma, these cells are comparatively poor at priming the expansion of IFN-γ responses from naïve CD4(+) T cells. The generation of MØP from transgenic or genetically aberrant mice provides an excellent opportunity to study the inflammatory role of GM-MØP, and reduces the need for mouse colonies in many studies. Hence differentiation of conditionally immortalised MØPs in GM-CSF represents a unique in vitro model of inflammatory monocyte-like cells, with important differences from bone marrow-derived dendritic cells, which will facilitate functional studies relating to the many 'sub-phenotypes' of inflammatory monocytes.
Infection, Immunity and Biochemistry, Cardiff University School of Medicine, Heath Park, Cardiff, Wales, UK.
Marcela
M
Rosas
Fabiola
F
Osorio
Matthew J
MJ
Robinson
Luke C
LC
Davies
Nicola
N
Dierkes
Simon A
SA
Jones
Caetano
C
Reis e Sousa
Philip R
PR
Taylor
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000954:Antigens, Surface / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology*; D002461:Cell Line, Transformed; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000235:genetics*; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D037181:Lectins, C-Type; D055666:Lipopeptides / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D055017:Monocyte-Macrophage Precursor Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology; D014161:Transduction, Genetic; D015054:Zymosan / Q000494:pharmacology; D047071:beta-Glucans / Q000494:pharmacology; D000099181:Dectin-1
D000954:Antigens, Surface; D016207:Cytokines; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D018398:Homeodomain Proteins; D007371:Interferon-gamma; D007376:Interleukin-2; D037181:Lectins, C-Type; D055666:Lipopeptides; D008070:Lipopolysaccharides; D016173:Macrophage Colony-Stimulating Factor; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D010047:Ovalbumin; D015054:Zymosan; D047071:beta-Glucans; D000099181:Dectin-1; C095822:Hoxb8 protein, mouse; C508075:Pam(3)CSK(4) peptide; C038459:curdlan