(1→3)-β-D-Glucan inhibits a dual mechanism of peroxynitrite stroke.
OBJECTIVE
The antioxidative and antinitrative activities of (1→3)-β-D-glucan (1-4μg/ml) from the yeast cell walls of Saccharomyces cerevisiae in human plasma treated with strong oxidants - peroxynitrite (ONOO(-)) (0.1mM) and hydrogen peroxide (H(2)O(2)) (2mM) were studied in vitro. The main purpose of this study was to assess if (1→3)-β-D-glucan, a well known strong immunostimulatory agent, possesses a protective function against dual mechanism of ONOO(-) stroke associated with nitrative and oxidative damages to human plasma biomolecules.


METHODS
The protein changes were determined in vitro by estimating the level of oxidative stress markers - carbonyl groups, and nitrative products - 3-nitrotyrosine residues. The plasma lipid peroxidation was also investigated. The obtained results show that (1→3)-β-D-glucan inhibits in vitro ONOO(-)-induced oxidation and nitration of plasma proteins, even by 50% and 30%, respectively. The antioxidative activity of (1→3)-β-D-glucan was confirmed by its inhibitory effect on plasma lipids peroxidation induced by ONOO(-) or by H(2)O(2).


CONCLUSIONS
The obtained results demonstrate that (1→3)-β-D-glucan from S. cerevisiae protects plasma components against toxic effects of ONOO(-) and H(2)O(2) due to its antioxidative and antinitrative activities. Therefore (1→3)-β-D-glucan supplementation during inflammatory may be beneficial not only regard for its ability to stimulate the immune system but also by antioxidative properties.
Department of General Biochemistry, University of Lodz, 90-237 Lodz, Banacha 12/16, Poland. juszczak@biol.uni.lodz.pl
Joanna
J
Saluk-Juszczak
Karolina
K
Krolewska
Barbara
B
Wachowicz
D000328:Adult; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001798:Blood Proteins / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D016877:Oxidants / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D030421:Peroxynitrous Acid / Q000378:metabolism / Q000494:pharmacology*; D010949:Plasma / Q000737:chemistry / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D055815:Young Adult; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D000975:Antioxidants; D001798:Blood Proteins; D016877:Oxidants; D047071:beta-Glucans; D030421:Peroxynitrous Acid; D006861:Hydrogen Peroxide