Expression of 14-3-3 σ protein in normal and neoplastic canine mammary gland.
14-3-3 σ protein is a negative cell cycle regulator, with both reduced and elevated levels associated with cancer in humans. This study assessed the expression of this protein in canine mammary tissues using immunohistochemistry and Western blotting. 14-3-3 σ was detected in 97% of the mammary tissue samples examined and was found in both myoepithelial (MECs) and epithelial (ECs) cells. Expression levels were elevated and reduced in neoplastic ECs and MECs, respectively (P<0.001). Intense expression of 14-3-3 σ was detected in neoplastic ECs infiltrating blood vessels and lymph nodes and suggests a possible role for this protein in the malignant transformation of mammary neoplasms. Moreover, double immunostaining for 14-3-3 σ and the MEC-specific marker p63, confirmed that 14-3-3 σ is a highly sensitive marker of MECs since all p63-positive cells were also positive for 14-3-3 σ. However, this protein is not exclusive to MECs as ECs also labelled positively.
Unit of Histology and Animal Pathology, Institute for Animal Health. Veterinary School, University of Las Palmas de Gran Canaria, 35413 Las Palmas, Spain. asuarez@becarios.ulpgc.es
Alejandro
A
Suárez-Bonnet
Pedro
P
Herráez
Juana
J
Martín de las Mulas
Francisco
F
Rodríguez
José M
JM
Déniz
Antonio
A
Espinosa de los Monteros
D048948:14-3-3 Proteins / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D009208:Myoepithelioma / Q000378:metabolism / Q000662:veterinary
D048948:14-3-3 Proteins; D015415:Biomarkers; D014408:Biomarkers, Tumor