Development and applications of whole cell biosensors for ecotoxicity testing.
Whole cell biosensors are the focus of considerable and increasing interest worldwide as methods for detecting and quantifying environmental toxicity, including biochemical oxygen demand (BOD), heavy metals, antibiotics, pesticides and herbicides. This review follows the development of whole cell biosensors from attempts to utilise changes in cellular metabolism to determine BOD and general toxicity, through the exploitation of unique metabolic pathways to detect specific toxicants, to the increasingly widespread use of genetic engineering to build new, and modify existing, sensing pathways.
Lincoln Ventures Ltd, Christchurch, New Zealand. pascon@lvl.co.nz
Neil F
NF
Pasco
Richard J
RJ
Weld
Joanne M
JM
Hay
Ravi
R
Gooneratne
D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002477:Cells / Q000378:metabolism*; D054750:Ecotoxicology / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D015386:Hazardous Substances / Q000032:analysis*
D015386:Hazardous Substances