Actinophytocola xinjiangensis sp. nov., isolated from virgin forest soil.
An aerobic, non-motile actinobacterium, strain QAIII60(T), was isolated from virgin forest soil of Kanas Nature Reserve, Xinjiang, north-west China. The isolate produced a very scant aerial mycelium that fragmented into cylindrical spores and a non-fragmented substrate mycelium with occasional septa. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose, galactose, glucose, ribose and rhamnose (trace). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids. The major cellular fatty acids were iso-C(16:0), iso-C(14:0), iso-C(16:1) H and C(17:1)ω6c. The isoprenoid quinones consisted of MK-9(H(4)) and MK-10(H(2)). The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QAIII60(T) formed a distinct phyletic line that was most closely, albeit loosely, associated with the genus Actinophytocola. A number of physiological characteristics differentiated the isolate from members of the genus Actinophytocola. On the basis of these data, we propose that strain QAIII60(T) (=CGMCC 4.4663(T) =NBRC 106673(T)) be assigned as the type strain of a novel species, Actinophytocola xinjiangensis sp. nov.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Xiaoxuan
X
Guo
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Danheng
D
Qiu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Jisheng
J
Ruan
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Ying
Y
Huang
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D014197:Trees / Q000254:growth & development
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S