[Screening and identification of differential expression genes related to stress fracture by cDNA microarray assay].
OBJECTIVE
To evaluate the differentially expressed genes between the Stress fracture (SF) cases and controls.


METHODS
Total RNA was extracted and purified from peripheral blood sample of 3 SF cases and 3 controls who conducted a 1:1 matched case-control study, then used for Human Genome Array analysis. The hybridization data were analyzed using SAM software. Parts of these genes were analyzed and identified by real-time PCR.


RESULTS
Upregulated and downregulated genes were 22 and 1, respectively. Thus the highest ratio and most significant cytokine was tumor necrosis factor receptor superfamily, member 10c (TNFRSF10C). The result of real-time PCR shows that TNFRSF10C was over-expressed in 3 cases and low-expressed in 1 case.


CONCLUSIONS
Obvious difference exists in gene expression between SF cases and controls, showing there may be a lot of genes involving in the occurrence and development of SF. Meanwhile, the identification of the specific genes is helpful for biomechanics study, early diagnosis and screening of SF.
Key Laboratory of Biomarkers for Occupational and Environmental Hazard, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China.
Hua-bing
HB
Zhao
Yi-zheng
YZ
Wang
Xiao-xia
XX
Lan
Ming-shun
MS
Zhang
Guo-ping
GP
Zhao
Guang-ya
GY
Yin
Shi-xin
SX
Wang
D016022:Case-Control Studies; D018076:DNA, Complementary / Q000235:genetics; D015775:Fractures, Stress / Q000097:blood* / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008889:Military Personnel; D020411:Oligonucleotide Array Sequence Analysis; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D053319:Tumor Necrosis Factor Decoy Receptors / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
D018076:DNA, Complementary; D058851:GPI-Linked Proteins; D053320:Receptors, Tumor Necrosis Factor, Member 10c; C108661:TNFRSF10C protein, human; D053319:Tumor Necrosis Factor Decoy Receptors