Chronopotentiometric technique as a method for electrical characterization of bilayer lipid membranes.
The basic electrical parameters of bilayer lipid membranes are capacitance and resistance. This article describes the application of chronopotentiometry to the research of lipid bilayers. Membranes were made from egg yolk phosphatidylcholine. The chronopotentiometric characteristic of the membranes depends on the current value. For low current values, no electroporation takes place and the voltage rises exponentially to a constant value. Based on these kinds of chronopotentiometric curves, a method of the membrane capacitance and the membrane resistance calculations are presented.
Institute of Chemistry, University of Bialystok, Al. J. Pilsudskiego 11/4, 15-443 Bialystok, Poland. monikan@uwb.edu.pl
Monika
M
Naumowicz
Zbigniew Artur
ZA
Figaszewski
D000818:Animals; D004530:Egg Yolk / Q000737:chemistry; D025802:Electric Capacitance; D017097:Electric Impedance; D004560:Electricity; D018274:Electroporation / Q000379:methods; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008432:Mathematical Computing; D008564:Membrane Potentials; D010713:Phosphatidylcholines / Q000378:metabolism*; D011199:Potentiometry / Q000379:methods*; D013997:Time Factors
D008051:Lipid Bilayers; D008055:Lipids; D010713:Phosphatidylcholines
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