The presence of TRAIL-OPG complex in human osteosarcoma and human salivary gland adenocarcinoma.
A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the human tumor necrosis factor-related apoptosis-induced ligand (TRAIL)-osteoprotegerin (OPG) complex was developed by utilizing a monoclonal antibody that recognizes human soluble TRAIL as an immobilized capture component and anti-human OPG immunoglobulin G (IgG) labeled with biotin. We could quantify the TRAIL-OPG complex level (detection limit: 10 pmol/L). Employing this assay system, we demonstrated that the TRAIL-OPG complex was constitutively present in the human osteosarcoma cell lines, HOS, MG-63, and human salivary gland adenocarcinoma cell line, HSG.
Department of Medicinal Biochemistry, School of Pharmacy, Aichi-Gakuin University, Nagoya, Japan. agumm7@yahoo.co.jp
Makio
M
Mogi
Ayami
A
Kondo
D000230:Adenocarcinoma / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D012516:Osteosarcoma / Q000276:immunology* / Q000378:metabolism; D053245:RANK Ligand / Q000032:analysis / Q000276:immunology*; D012468:Salivary Gland Neoplasms / Q000276:immunology*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D053245:RANK Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand