Protein composition analysis of oil bodies from maize embryos during germination.
Seed oil bodies (OBs) are intracellular particles that store lipids. In maize embryos, the oil bodies are accumulated mainly in the scutellum. Oil bodies were purified from the scutellum of germinating maize seeds and the associated proteins were extracted and subjected to 2-DE analysis followed by LC-MS/MS for protein identification. In addition to the previously known oil body proteins oleosin, caleosin and steroleosin, new proteins were identified.
Department of Molecular Genetics, Centre for Research in Agrigenomics CSIC-IRTA-UAB, Jordi Girona 18-26, Barcelona 08034, Spain.
Hedia
H
Tnani
Ignacio
I
López
Thierry
T
Jouenne
Carlos M
CM
Vicient
D015153:Blotting, Western; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D018525:Germination; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000196:embryology* / Q000502:physiology
D010940:Plant Proteins