Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation.
Dentin matrix metalloproteinases (MMPs) are involved in the degradation of collagen in resin-dentin interfaces. This study evaluated whether collagen degradation can be prevented by chlorhexidine digluconate (CHX) after different dentin demineralization procedures. The demineralization of human dentin was performed with phosphoric acid (PA), EDTA or acidic monomers (Clearfil SE Bond and Xeno V). Specimens were stored (for 24 h, or for 1 or 3 wk) in the presence or absence of CHX. In half of the groups, active MMP-2 was incorporated into the storage solution. At the end of each storage period, the C-terminal telopeptide (ICTP) concentration (which indicates the amount of collagen degradation) was measured in the storage solution. Collagen degradation was higher in PA- and EDTA-demineralized dentin. Chlorhexidine digluconate reduced collagen degradation in these groups only for 24 h. When dentin was demineralized with Clearfil SE Bond or Xeno V, collagen degradation was reduced by up to 30%, but the addition of exogenous MMP-2 significantly increased collagen degradation. In self-etchant-treated dentin, the inhibitory effect of CHX on MMPs lasted for up to 3 wk. Treating dentin with EDTA, PA or self-etching agents produces enough demineralization to permit cleavage of the exposed collagen. Monomer infiltration may exert protection on demineralized collagen, probably through immobilization of MMPs. The partial inhibitory action of CHX on MMP activity produced by self-etching adhesives was prolonged compared with the short-acting PA- or EDTA-treated dentin.
Department of Dental Materials, School of Dentistry, University of Granada, Granada, Spain. toledano@ugr.es
Raquel
R
Osorio
Mónica
M
Yamauti
Estrella
E
Osorio
María E
ME
Ruiz-Requena
David
D
Pashley
Franklin
F
Tay
Manuel
M
Toledano
D000134:Acid Etching, Dental / Q000379:methods*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D024042:Collagen Type I / Q000032:analysis / Q000737:chemistry*; D001840:Dental Bonding; D003799:Dental Stress Analysis; D003804:Dentin / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D010455:Peptides / Q000032:analysis; D011480:Protease Inhibitors / Q000494:pharmacology*; D019279:Resin Cements; D013718:Tensile Strength; D017001:Tooth Demineralization / Q000201:enzymology / Q000517:prevention & control*; D055815:Young Adult
D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D010455:Peptides; D011480:Protease Inhibitors; D019279:Resin Cements; C052929:collagen type I trimeric cross-linked peptide; C010882:chlorhexidine gluconate; D002710:Chlorhexidine
Inflamm Res. 2010 May;59(5):379-89
19902332
J Dent Res. 2009 Jan;88(1):77-82
19131322
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005 Dec;100(6):756-61
16301159
Int Endod J. 2009 Apr;42(4):288-302
19220510
Biomaterials. 2004 Aug;25(17):3743-50
15020150
ChemMedChem. 2009 Feb;4(2):164-70
19072819
Biomaterials. 2009 Apr;30(10):1890-7
19147221
Reproduction. 2002 Aug;124(2):235-40
12141936
J Bone Miner Res. 2003 May;18(5):859-67
12733725
Biochim Biophys Acta. 2010 Jan;1803(1):20-8
19374923
Biomaterials. 2006 Sep;27(25):4470-6
16687171
Biomaterials. 2003 Sep;24(21):3795-803
12818552
J Pharm Pharmacol. 1980 Aug;32(8):525-32
6106686
J Dent Res. 2007 May;86(5):436-40
17452564
J Dent Res. 2004 Mar;83(3):216-21
14981122
Dent Mater. 2010 Aug;26(8):771-8
20472280
J Biochem Biophys Methods. 1991 Jul-Aug;23(1):31-43
1918799
J Endod. 2006 Sep;32(9):862-8
16934629
J Dent Res. 2005 Aug;84(8):741-6
16040733
Exp Cell Res. 2005 Apr 1;304(2):493-505
15748894
Eur J Oral Sci. 2005 Aug;113(4):341-8
16048527
J Dent Res. 2006 Jan;85(1):22-32
16373676
J Pharm Pharmacol. 1980 Jul;32(7):453-9
6105179
Eur J Oral Sci. 2009 Feb;117(1):64-7
19196320
Eur J Oral Sci. 2006 Apr;114(2):160-6
16630309
J Biomed Mater Res B Appl Biomater. 2009 Jul;90(1):373-80
19090493
J Dent. 2009 Oct;37(10):807-12
19615807
Dent Mater. 2010 Apr;26(4):320-5
20045177
Circ Res. 2003 May 2;92(8):827-39
12730128
J Nutr. 2000 May;130(5S Suppl):1437S-46S
10801957
J Pharm Pharmacol. 1981 Feb;33(2):82-8
6111604
Arch Oral Biol. 1996 Apr;41(4):369-77
8771328
J Dent Res. 2009 Dec;88(12):1101-6
19861692
Clin Chem. 2001 Aug;47(8):1410-4
11468230
J Biol Chem. 1999 Jul 30;274(31):21491-4
10419448
J Dent Res. 2010 Dec;89(12):1499-504
20940353
Eur J Oral Sci. 2004 Aug;112(4):376-83
15279658
Am J Infect Control. 2009 Sep;37(7):569-73
19398245
J Biomater Sci Polym Ed. 2008;19(1):19-29
18177551
J Med Chem. 2009 Jun 11;52(11):3523-38
19422229
J Dent Res. 1998 Aug;77(8):1622-9
9719036
Anal Bioanal Chem. 2007 Sep;389(2):355-68
17546446
J Biomed Mater Res B Appl Biomater. 2010 Jul;94(1):134-40
20524187