[Simultaneous determination of nine isoflavonoids in Huangqi Gegen decoction by high performance liquid chromatography].
OBJECTIVE
To develop a reversed-phase high performance liquid chromatographic method (RP-HPLC) for the simultaneous analysis of nine isoflavonoids in Huangqi Gegen decoction.


METHODS
HPLC determination was performed on a Kromasil C18 column(4.6 mm x 250 mm, 5 microm) and detected at 250 nm. The mobile phase was consisted of methanol and 0.1% citric acid solution with gradient elution. Flow-rate was 1.0 mL x min(-1), and column temperature was 25 degrees C.


RESULTS
The method was proved to be linear in the ranges of 81.28-406.4, 670.4-3 352, 136.96-684. 8, 33.376-166.88, 22.848-114.24, 10.176-50.88, 31.04-155.2, 44.544-222.72, 26.56-132.8 ng for the nine isoflavonoid, 3'-hydroxypuerarin, puerarin, daidzin, calycosin-7-O-beta-D-glucoside, genistin, ononin, daidzein, calycosin and formononetin respectively. The average recoveries were 99.27%, 102.38%, 98.46%, 103.06%, 101.29%, 99.71%, 102.28%, 97.89% and 100.78%, respectively, RSD was 1.8%, 2.0%, 1.4%, 1.4%, 1.3%, 1.8%, 1.3%, 0.97% and 1.8%, respectively.


CONCLUSIONS
Results obtained show that it's a convenient, accurate and reliable method. The method has been successfully applied to the simultaneous determination of the nine isoflavonoids in Huangqi Gegen decoction.
School of Chinese materia medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. wzping_jshb@126.com
Zhiping
Z
Wang
Weimin
W
Li
Ying
Y
Gao
Jie
J
Liu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis*
D004365:Drugs, Chinese Herbal; C439205:Huangqin-Tang; D007529:Isoflavones