A simple head-mountable LED device for chronic stimulation of optogenetic molecules in freely moving mice.
We describe a low-cost, small, remotely triggerable LED device for wireless control of transcranial optical stimulation of cortical neurons, for use in freely moving mice. The device is easily mountable on the head of a mouse with a high-polymer block. Using the Thy1-ChR2-YFP transgenic mice, we demonstrate that the device is capable of remotely triggering muscle twitches upon activation of the primary motor cortex in freely moving conditions.
Hirase Research Unit, RIKEN Brain Science Institute, Wako, Saitama, Japan.
Youichi
Y
Iwai
Shinzou
S
Honda
Hirofumi
H
Ozeki
Mitsuhiro
M
Hashimoto
Hajime
H
Hirase
D000818:Animals; D004582:Electronics, Medical / Q000295:instrumentation* / Q000379:methods*; D019054:Evoked Potentials, Motor / Q000502:physiology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009044:Motor Cortex / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D010775:Photic Stimulation / Q000295:instrumentation* / Q000379:methods*; D017966:Pyramidal Cells / Q000502:physiology / Q000528:radiation effects; D056969:Voltage-Sensitive Dye Imaging / Q000295:instrumentation* / Q000379:methods*