Relationships among hemocytes, tunic cells, germ cells, and accessory cells in the colonial ascidian Botryllus schlosseri.
Monoclonal antibodies were raised against hemocytes of the colonial ascidian Botryllus schlosseri as possible tools to study hemocyte differentiation. In this species, blood cells are involved in various biological functions, such as immunosurveillance, encapsulation of foreign bodies, metal accumulation, and allorecognition. The latter process drives the fusion or rejection of contacting colonies, according to whether they do or do not share at least one allele at the fusibility/histocompatibility (Fu/HC) locus. Hemocytes take part in the rejection reaction, which suggests that they express molecules, coded by the Fu/HC locus, on their surface. A homozygous colony at the Fu/HC locus was used to produce the antibodies, which were screened by immunocytochemistry on hemocyte monolayers, immunohistochemistry on colony paraffin sections, and immunoblotting on colony homogenates. Here, we report on one of the obtained antibodies (1D8), which recognized a surface epitope on hemocytes of the donor colony and other colonies, apparently in a manner specific to the Fu/HC genotype. It also labeled a single 80-kDa band in colony homogenates. In addition, it specifically recognized tunic cells, germ cells, and their accessory cells. These results strengthen the assumption of a close relationship among these types of cells and blood cells, and suggest a close relationship among the above cells, probably deriving from undifferentiated blood cells.
Department of Biology, University of Padova, Padova, Italy. loriano.ballarin@unipd.it
Loriano
L
Ballarin
Marcello
M
Del Favero
Lucia
L
Manni
D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation / Q000276:immunology / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005854:Germ Cells / Q000276:immunology / Q000502:physiology*; D006434:Hemocytes / Q000276:immunology / Q000502:physiology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D014561:Urochordata / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal