High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons.
The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders.
Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania, United States of America.
Satish L
SL
Deshmane
Ruma
R
Mukerjee
Shongshan
S
Fan
Bassel E
BE
Sawaya
D015526:AIDS Dementia Complex / Q000209:etiology; D002450:Cell Communication; D002460:Cell Line; D019075:Electrophoresis, Capillary / Q000379:methods*; D015696:Gene Products, tat / Q000032:analysis* / Q000097:blood; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D009474:Neurons / Q000737:chemistry / Q000821:virology*
D015696:Gene Products, tat
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