Molecular phylogenetic analysis of Enterobius vermicularis and development of an 18S ribosomal DNA-targeted diagnostic PCR.
We genetically characterized pinworms obtained from 37 children from different regions of Germany and established new species-specific molecular diagnostic tools. No ribosomal DNA diversity was found; the phylogenetic position of Enterobius vermicularis within the Oxyurida order and its close relationship to the Ascaridida and Spirurida orders was confirmed.
Molecular Parasitology Unit, Institute for Tropical Medicine, University of Tübingen, Wilhelmstrasse 27, D-72074 Tübingen, Germany.
Ulrike E
UE
Zelck
Ralf
R
Bialek
Michael
M
Weiss
D000818:Animals; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017229:Enterobiasis / Q000175:diagnosis* / Q000469:parasitology; D004757:Enterobius / Q000235:genetics* / Q000302:isolation & purification*; D005858:Germany; D006801:Humans; D008969:Molecular Sequence Data; D010274:Parasitology / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
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