The pro-inflammatory role of adiponectin at the maternal-fetal interface.
OBJECTIVE
A successful pregnancy is contingent on maternal tolerance of the immunologically foreign fetus. Prevalent diseases such as preeclampsia arise in part due to an inappropriate immune response by the placenta. A number of molecules have been proposed to temper cellular response to pro-inflammatory mediators, including CD24 and Siglec10.


METHODS
Cytotrophoblast cells from healthy term placentas were treated with adiponectin in vitro and analyzed with qPCR and ELISA-based assays. Immunohistochemistry was performed on term villous sections and cultured trophoblasts.


RESULTS
Treatment with adiponectin increased expression of IL-1β and IL-8. Term villi express CD24 in cytotrophoblasts and the syncytiotrophoblast, and Siglec10 by the syncytiotrophoblast. Treatment of trophoblast cells with adiponectin increased Siglec10 expression.


CONCLUSIONS
These data describe a role for adiponectin in enhancing pro-inflammatory signals in in vitro syncytialized trophoblasts. Additionally, this represents the first time the CD24/Siglec10 pathway has been implicated in a trophoblast response to a pro-inflammatory mediator.
Department of Molecular and Integrative Physiology, The Institute for Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, 3901 Rainbow Blvd., Kansas City, KS 66160, USA.
Emily A
EA
McDonald
Michael W
MW
Wolfe
D052242:Adiponectin / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008431:Maternal-Fetal Exchange / Q000276:immunology*; D010920:Placenta / Q000166:cytology / Q000276:immunology; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology
D052242:Adiponectin; D016207:Cytokines; D012333:RNA, Messenger