[Molecular-cellular characteristic of blood lymphocytes in Hodgkin lymphoma].
The molecular-cellular parameters complex has been studied on the blood lymphocytes of malignant Hodgkin's lymphoma (HL) patients: the frequency of cells with micronuclei (MN) and chromosome aberrations; the level of DNA single and double strand breaks - OR and DR DNA (DNA comet assay), oxidative status--the content of reactive oxygen species (ROS) by using nonfluorescent dye that is oxygenated in the cells to fluorescent reagent and detection of fluorescence intensity after there. It was shown that the patients with LH had the increased level of DR and OR DNA, the increased frequency of cells with chromosome aberrations and the number of aberrations per cell was increased too. The concentration of ROS is increased too for the most individuals with intoxication. In the process of the chemical and radiation therapy the increase of OR DNA level, the frequency of the cell with MN has been registered. The ROS concentration correlates with the level of DNA-strand breaks. So the blood lymphocytes of HL patients before treatment differ from the lymphocytes of healthy donors. The damage of genome and the change of oxidative status have been observed that can be additive markers for the HL diagnosis, their sensitivity to the treatment and the characteristic of lymphocytes changes by this disease.
V V
VV
Pavlov
A V
AV
Aleshchenko
M M
MM
Antoshchina
T M
TM
Blochina
O V
OV
Kudriashova
O E
OE
Lashkova
E Iu
EIu
Lizunova
A N
AN
Osipov
N I
NI
Riabchenko
A M
AM
Serebrianyĭ
I I
II
Pelevina
D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis; D004249:DNA Damage; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D006801:Humans; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D048629:Micronuclei, Chromosome-Defective; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species