Taurine supplementation decreases oxidative stress in skeletal muscle after eccentric exercise.
Infrequent exercise, typically involving eccentric actions, has been shown to cause oxidative stress and to damage muscle tissue. High taurine levels are present in skeletal muscle and may play a role in cellular defences against free radical-mediated damage. This study investigates the effects of taurine supplementation on oxidative stress biomarkers after eccentric exercise (EE). Twenty-four male rats were divided into the following groups (n = 6): control; EE; EE plus taurine (EE + Taurine); EE plus saline (EE + Saline). Taurine was administered as a 1-ml 300 mg kg(-1) per body weight (BW) day(-1) solution in water by gavage, for 15 consecutive days. Starting on the 14th day of supplementation, the animals were submitted to one 90-min downhill run session and constant velocity of 1·0 km h(-1) . Forty-eight hours after the exercise session, the animals were killed and the quadriceps muscles were surgically removed. Production of superoxide anion, creatine kinase (CK) levels, lipoperoxidation, carbonylation, total thiol content and antioxidant enzyme were analysed. Taurine supplementation was found to decrease superoxide radical production, CK, lipoperoxidation and carbonylation levels and increased total thiol content in skeletal muscle, but it did not affect antioxidant enzyme activity after EE. The present study suggests that taurine affects skeletal muscle contraction by decreasing oxidative stress, in association with decreased superoxide radical production.
Exercise Biochemistry and Physiology Laboratory, Postgraduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil. luciano_acordi@yahoo.com.br
Luciano A
LA
Silva
Paulo C L
PC
Silveira
Merieli M
MM
Ronsani
Priscila S
PS
Souza
Débora
D
Scheffer
Lílian C
LC
Vieira
Magnus
M
Benetti
Cláudio T
CT
De Souza
Ricardo A
RA
Pinho
D000818:Animals; D003402:Creatine Kinase / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism; D013481:Superoxides / Q000032:analysis / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*
D013438:Sulfhydryl Compounds; D013481:Superoxides; D013654:Taurine; D003402:Creatine Kinase