Adipogenic differentiation and EGFP gene transfection of amniotic fluid-derived stem cells from goat fetus at terminal gestational age.
The aims of this study were to determine whether stem cells could be isolated from amniotic fluid of goat fetus at terminal gestational age and to determine if these stem cells could differentiate into adipogenic cells and be transfected with a reporter gene, EGFP (enhanced green fluorescent protein). The stem cells were isolated from amniotic fluid of goat fetus at terminal gestational age, induced to differentiate into adipogenic cells in vitro and transfected with the EGFP gene using lipofection. Markers associated with undifferentiated AFS (amniotic fluid-derived stem) cells were tested by RT (reverse transcription)-PCR. The results demonstrated that AFS cells could be isolated from amniotic fluid of goat fetus at terminal gestational age and could differentiate into adipogenic cells. The EGFP gene was transfected into AFS cells successfully. EGFP gene transfection efficiency of the three groups of transgenic AFS cells were 26.0, 29.9 and 30.5%, respectively. Both transgenic and wild-type AFS cells could express Hes1 (hairy and enhancer of split 1), Oct4 (octamer-binding protein 4) and Nanog.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, Peoples Republic of China.
Xiao-Ying
XY
He
Yue-Mao
YM
Zheng
Shuang
S
Qiu
Ying-Pei
YP
Qi
Yong
Y
Zhang
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006041:Goats; D049452:Green Fluorescent Proteins / Q000235:genetics*; D018398:Homeodomain Proteins / Q000096:biosynthesis; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D004268:DNA-Binding Proteins; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; C485184:enhanced green fluorescent protein