[Comparison of rutin and syringin content between tissue culturing seedlings and botanical drug of Saussurea involucrata].
OBJECTIVE
To compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.


METHODS
The HPLC with Hydro-RP C18 (4.6 mm x 250 mm, 5 microm) column was used, a mixture of acetonitrile-water (5:95) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 25 degrees C and detection wavelength at 220 nm.


RESULTS
The effective constituents of tissue culturing seedlings were almost similar to the botanical drug. And syringin in tissue culturing seedlings was increased 4.35 times.


CONCLUSIONS
It has a good prospect to acquire high-quality S. involucrata by tissue culturing seedlings.
Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College, Beijing 100193, China.
Yali
Y
Lv
Chunlan
C
Wang
Shunxing
S
Guo
Hailing
H
Dong
D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D010666:Phenylpropionates / Q000032:analysis*; D012431:Rutin / Q000032:analysis*; D030441:Saussurea / Q000737:chemistry* / Q000254:growth & development; D036226:Seedlings / Q000737:chemistry
D005960:Glucosides; D010666:Phenylpropionates; D012431:Rutin; C028305:syringin