Proteome analysis of the left ventricle in the vitamin D₃ and nicotine-induced rat vascular calcification model.
Vitamin D₃ and nicotine (VDN) serve as an animal model of arterial calcification. The vascular calcification induced by the VDN model is always accompanied by compensatory left ventricular (LV) hypertrophy and impaired cardiac performance. To determine the possible mechanisms that are responsible for the effects of VDN on the LV, a 2-DE based proteomics approach was used to evaluate the changes in protein expression of the left ventricle in VDN rats, to our knowledge, for the first time. We identified sixteen proteins that were markedly altered and involved in mitochondrial function, heat shock protein activity, myocyte cytoskeleton composition and enzyme activity for energy metabolism. We describe, for the first time, a novel pathway (NDPK) that is involved in LV hypertrophy and enzyme activities of three of the sixteen clinical identified proteins: lactate dehydrogenase (LDH), SOD [Mn] and GST.
National Key Research laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, 100083, PR China.
Qian-Qian
QQ
Wang
Xin
X
Zhao
Xiao-Ping
XP
Pu
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002114:Calcinosis / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D009202:Cardiomyopathies / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002762:Cholecalciferol; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D006352:Heart Ventricles / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009538:Nicotine; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome; D002762:Cholecalciferol; D009538:Nicotine