Temporal expression and tissue distribution of interleukin-1β in two strains of guinea pigs with varying propensity for spontaneous knee osteoarthritis.
OBJECTIVE
To provide a comprehensive immunohistochemical (IHC) map of the temporal expression and tissue distribution of interleukin-1β (IL-1β) through progression of osteoarthritis (OA) in two strains of guinea pigs with varying propensity for spontaneous knee joint disease.


METHODS
OA-prone Hartley and OA-resistant Strain 13 guinea pigs were collected at 60, 120, 180, 240, 360, and 480 days of age (N=4 animals per strain per date). IHC was performed on whole joint preparations; the distribution of IL-1β expression on coronal sections was mapped, semi-quantitatively scored, and correlated to OA grade using Mankin criteria with guinea pig-specific modifications. OA and IHC indices were compared among times and between strains using the Kruskal-Wallis one-way analysis of variance by ranks followed by Dunn's post test.


RESULTS
OA indices for both strains increased from 60 to 480 days of age; a statistically higher score (P ≤ 0.01) was found in Hartley animals at 180, 240, 360, and 480 days. At 60 days of age, IL-1β expression was detected in cartilage, menisci, synovium, and subchondral bone in both strains. Persistent and statistically increased (P<0.05) IL-1β expression was found in these same tissues in Hartley animals at 120 and 180 days, while Strain 13 animals demonstrated a significant reduction in positive immunostaining. Statistical differences in IHC indices between strains beyond 240 days of age were restricted to synovium (days 240 and 480) and subchondral bone (days 360 and 480).


CONCLUSIONS
As expected, histologic OA proceeded in an accelerated manner in Hartley animals relative to Strain 13 animals. The OA-prone strain did not demonstrate reduced IL-1β expression during adult maturity as occurred in the OA-resistant strain, and this persistent expression may have corresponded to early incidence of OA. Future interventional studies are warranted to explore whether dysregulation of IL-1β expression may contribute to premature onset of spontaneous disease in the Hartley guinea pig.
Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA.
K S
KS
Santangelo
E M
EM
Pieczarka
G J
GJ
Nuovo
S E
SE
Weisbrode
A L
AL
Bertone
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism; D006168:Guinea Pigs; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000378:metabolism*; D007719:Knee Joint / Q000378:metabolism* / Q000473:pathology; D020370:Osteoarthritis, Knee / Q000378:metabolism* / Q000473:pathology; D013583:Synovial Membrane / Q000378:metabolism
D053583:Interleukin-1beta
J Histochem Cytochem. 1994 Jun;42(6):733-44
8189035
Bull NYU Hosp Jt Dis. 2007;65(3):242-8
17922676
Int J Biochem Cell Biol. 2005 Jan;37(1):224-36
15381164
Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2735-40
19193853
J Orthop Res. 1995 May;13(3):399-409
7541454
J Rheumatol. 1998 Dec;25(12):2417-24
9858439
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S35-52
20864022
Clin Orthop Relat Res. 2004 Oct;(427 Suppl):S37-46
15480072
Arthritis Rheum. 2009 Mar 15;61(3):344-52
19248129
Arthritis Rheum. 1996 Sep;39(9):1535-44
8814066
J Orthop Res. 2008 Feb;26(2):231-7
17868097
Osteoarthritis Cartilage. 2010 Mar;18(3):289-96
19857509
Osteoarthritis Cartilage. 2010 Mar;18(3):397-405
19825496
Osteoarthritis Cartilage. 2002 Oct;10(10):758-67
12359161
Arthritis Rheum. 1999 Mar;42(3):498-506
10088773
Clin Orthop Relat Res. 2004 Oct;(427 Suppl):S27-36
15480070
Gene Ther. 2005 Jan;12(2):177-86
15578043
Ann Rheum Dis. 2004 Dec;63(12):1611-7
15331394
Eur Cell Mater. 2006 Oct 26;12:49-56; discussion 56
17068722
Gene Ther. 2004 Feb;11(4):379-89
14724685
Rheum Dis Clin North Am. 2002 Feb;28(1):127-49
11840694
Arthritis Rheum. 2001 Jun;44(6):1237-47
11407681
Arthritis Rheum. 2001 Mar;44(3):585-94
11263773
Cytokine. 2004 Oct 7;28(1):17-24
15341921
Am J Pathol. 2007 Sep;171(3):938-46
17640966
Arthritis Rheum. 2003 Dec;48(12):3304-7
14673981
Arthritis Rheum. 2003 Dec;48(12):3452-63
14673996
Receptor. 1994 Spring;4(1):9-15
8038709
Orthop Rev (Pavia). 2009 Oct 10;1(2):e14
21808676
Osteoarthritis Cartilage. 2006 Sep;14(9):923-30
16679035
Arthritis Rheum. 1998 May;41(5):877-90
9588741
Arthritis Rheum. 1997 Jun;40(6):1012-9
9182910
Gene Ther. 2002 Jan;9(1):12-20
11850718