[Analysis of common wheat varieties and near-isogenic lines by PCR with allele-specific primers for Gli-1 and Glu-3 loci].
The allelic characteristics of the Gli-A1, Gli-B1, Gli-D1 and Glu-A3 loci of 14 bread wheat varieties and 6 near-isogenic lines derived from Bezostaya 1 have been detected by PCR analysis. The conformity of molecular-genetic data and electrophoresis of storage proteins has been determined: the allelic variants of gliadins Gli-A1o and Gi-A1m correspond to the PCR-allele GliA1.2, the gliadin variants Gli-A1f, Gli-A1b, Gli-A1c correspond to the PCR-allele GliA 1.1, the allelic variants Gli-B1b, Gli-B1d--to the PCR-allele GliB1.1 and the variants Gli-B1e, Gli-B1g, Gli-B1c-to the PCR-allele GliB1.2. A new PCR-allele at the GliB) locus in the line Gli-B1-12 (with the gliadin block Gli-B1o from Levent) was identified.
A M
AM
Polishchuk
S V
SV
Chebotar
O M
OM
Blahodarova
N O
NO
Kozub
I O
IO
Sozinov
Iu M
IuM
Syvolap
D000483:Alleles; D003433:Crosses, Genetic; D017931:DNA Primers; D005903:Gliadin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D005903:Gliadin