Allyl isothiocyanate regulates caspase-1/receptor interacting protein-2 expression.
Isothiocyanates can inhibit nuclear factor-kappaB (NF-kappaB) activation. Interleukin (IL)-1β activates the NF-kappaB, which in turn activates proteins involved in inflammation. IL-1β is directly associated with caspase-1 activation. We tested the anti-inflammatory effect of allyl isothiocyanate (AITC) in mast cells. AITC suppressed the intracellular calcium level in the phorbol myristate acetate (PMA) plus calcium ionophore A23187-stimulated human mast cell line. AITC decreased PMA plus A23187-induced cystein-aspartic acid protease (caspase)-1 activity. Particularly, AITC decreased PMA plus A23187-induced caspase-1/receptor interacting protein-2 expression as well as the mRNA expression and production of IL-1β. An in-depth research of the cellular targets of the AITC is warranted.
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Na-Ra
NR
Han
Wansu
W
Park
Jae-Young
JY
Um
Hyung-Min
HM
Kim
Hyun-Ja
HJ
Jeong
D000498:Allyl Compounds / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D020170:Caspase 1 / Q000096:biosynthesis*; D018929:Cell Culture Techniques; D002460:Cell Line; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000276:immunology; D017953:Isocyanates / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
D000498:Allyl Compounds; D000894:Anti-Inflammatory Agents, Non-Steroidal; D053583:Interleukin-1beta; D017953:Isocyanates; C491149:allyl isocyanate; C506253:RIPK2 protein, human; D053475:Receptor-Interacting Protein Serine-Threonine Kinase 2; D020170:Caspase 1; D002118:Calcium