Ultrasound irradiation promoted efficient solvent-free lipase-catalyzed production of mono- and diacylglycerols from olive oil.
This work reports the enzymatic production of mono- and diacylglycerols under the influence of ultrasound irradiation, in a solvent-free system, with and without the presence of surfactants at a constant temperature of 65°C, glycerol to oil molar ratio of 2:1 and a commercial immobilized lipase (Novozym 435) as catalyst. For this purpose, two operation modes were adopted: the use of a sonotrode (ultrasonic probe), without agitation, varying reaction time, irradiation amplitude (25-45% of the total power) and type of surfactant, and a mechanically stirred reactor (600 rpm) under ultrasound irradiation in a water bath, testing different surfactants. Results show that very satisfactory MAG and DAG yields, above 50 wt.%, can be obtained without the use of surfactant, at mild irradiation power supply (∼130 W), with no important enzyme activity losses verified, in a relatively short reaction time (2h), and low enzyme content (7.5 wt.%). Also, reaction kinetic results show that contents of MAG+DAG as high as ∼65 wt.% can be achieved at longer times (6h), indicating a promising route for producing MAG and DAG using ultrasound irradiation.
Department of Food Engineering, URI-Campus de Erechim, Erechim, RS, Brazil.
Karina G
KG
Fiametti
Melania M
MM
Sychoski
Aline
A
De Cesaro
Agenor
A
Furigo
Lizandra C
LC
Bretanha
Claudio M P
CM
Pereira
Helen
H
Treichel
Débora
D
de Oliveira
J Vladimir
JV
Oliveira
D004075:Diglycerides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000528:radiation effects*; D005511:Food Handling / Q000379:methods*; D008049:Lipase / Q000737:chemistry; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry* / Q000528:radiation effects*; D011829:Radiation Dosage; D012997:Solvents / Q000737:chemistry; D013010:Sonication / Q000379:methods*
D004075:Diglycerides; D000069463:Olive Oil; D010938:Plant Oils; D012997:Solvents; D008049:Lipase