Control of RNA processing by a large non-coding RNA over-expressed in carcinomas.
RNA processing is vital for the high fidelity and diversity of eukaryotic transcriptomes and the encoded proteomes. However, control of RNA processing is not fully established. Σ RNA is a class of conserved large non-coding RNAs (murine Hepcarcin; human MALAT-1) up-regulated in carcinomas. Using antisense technology, we identified that RNA post-transcriptional modification is the most significant global function of Σ RNA. Specifically, processing of the pre-mRNAs of genes including Tissue Factor and Endoglin was altered by hydrolysis of Σ RNA/MALAT-1. These results support the hypothesis that Σ RNA/MALAT-1 is a regulatory molecule exerting roles in RNA post-transcriptional modification.
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA. ruilin2005@gmail.com
Rui
R
Lin
Manami
M
Roychowdhury-Saha
Chris
C
Black
Andrew T
AT
Watt
Eric G
EG
Marcusson
Susan M
SM
Freier
Thomas S
TS
Edgington
D017398:Alternative Splicing; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism*; D002478:Cells, Cultured; D000071063:Endoglin; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016376:Oligonucleotides, Antisense; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm / Q000378:metabolism*; D022661:RNA, Untranslated / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D000068103:Serine-Arginine Splicing Factors; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; C557041:MALAT1 long non-coding RNA, human; C557040:Malat1 long non-coding RNA, mouse; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; D012322:RNA Precursors; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D011956:Receptors, Cell Surface; D000068103:Serine-Arginine Splicing Factors; D013925:Thromboplastin
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