Aquisphaera giovannonii gen. nov., sp. nov., a planctomycete isolated from a freshwater aquarium.
As part of a study of the diversity of planctomycetes, two novel strains, designated OJF2(T) and OJF8, were isolated from the sediments of a freshwater aquarium. The organisms were chemoheterotrophic, spherical and pink-pigmented, had an optimum growth temperature of about 30-35 °C and an optimum pH for growth of around 7.5-8.5. The predominant fatty acids were C(18:1)ω9c and C(16:0). The two strains were able to assimilate several sugars and organic acids. 16S rRNA gene sequence analysis confirmed the affiliation of these organisms to the phylum 'Planctomycetes'; they showed highest similarity to the type strains of Singulisphaera acidiphila (92.4%) and Isosphaera pallida (91.9%). On the basis of physiological, biochemical and chemotaxonomic characteristics, strains OJF2(T) and OJF8 are considered to represent a novel species of a new genus of the order Planctomycetales, for which the name Aquisphaera giovannonii gen. nov., sp. nov. is proposed. The type strain of Aquisphaera giovannonii is OJF2(T) (=CECT 7510(T) =DSM 22561(T)).
CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.|Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/no, 4169-007 Porto, Portugal.
Joana
J
Bondoso
Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
Luciana
L
Albuquerque
Departamento de Ciências da Vida, Apartado 3046, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
M Fernanda
MF
Nobre
Laboratório de Biologia Celular, Instituto de Ciências Biomédicas Abel Salazar, ICBAS, Universidade do Porto, Largo Abel Salazar 2, 4099-003 Porto, Portugal.|CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.
Alexandre
A
Lobo-da-Cunha
Departamento de Ciências da Vida, Apartado 3046, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
Milton S
MS
da Costa
CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.|Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/no, 4169-007 Porto, Portugal.
Olga Maria
OM
Lage
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S