JAK2/STAT3 pathway is involved in the early stage of adipogenesis through regulating C/EBPβ transcription.
Signal transducer and activator of transcription 3 (STAT3) was reported to be involved in adipogenesis. However, the regulating mechanism of STAT3 remains unclear. The present results showed that STAT3 was activated within 2-h adipogenic induction, in which the phosphorylated STAT3 translocated from cytoplasm to the nucleus. In addition, we detected Janus kinase2 (JAK2) acted upstream of the STAT3 activation at the early stage of adipogenesis. Accordingly, the JAK2 inhibitor AG490 and siRNAs led to the partial inhibition of the STAT3 activation, and the inhibition of 3T3-L1 adipocyte differentiation. Furthermore, the results based on luciferase, chromatin immunoprecipitation, and gel shift approaches indicated that STAT3 could regulate the transcription of C/EBPβ by binding the distal region of C/EBPβ promoter at the early stage of adipogenesis. Collectively, our findings reveal that JAK2/STAT3 pathway is involved in the early stage of 3T3-L1 adipocyte differentiation though regulating the C/EBPβ transcription.
Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Kunming
K
Zhang
Wei
W
Guo
Ying
Y
Yang
Jiarui
J
Wu
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D003907:Dexamethasone / Q000494:pharmacology; D024202:Electrophoretic Mobility Shift Assay; D007328:Insulin / Q000494:pharmacology; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D020032:Tyrphostins / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
D022782:CCAAT-Enhancer-Binding Protein-beta; D007328:Insulin; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; D020032:Tyrphostins; D014970:Xanthines; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; C008514:methylxanthine; D003907:Dexamethasone; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2