Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition.
The effect of different solvents and pollutants on the cellular fatty acid composition of three bacterial strains: Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans, representatives of diverse predominant anaerobic metabolisms was investigated. As the prevailing adaptive mechanism in cells of T. aromatica and G. sulfurreducens whose cellular fatty acids patterns were dominated by palmitic acid (C16:0) and palmitoleic acid (C16:1cis), the cells reacted by an increase in the degree of saturation of their membrane fatty acids when grown in the presence of sublethal concentrations of the chemicals. Next to palmitic acid C16:0, the fatty acid pattern of D. multivorans was dominated by anteiso-branched fatty acids which are characteristic for several sulfate-reducing bacteria. The cells responded to the solvents with an increase in the ratio of straight-chain saturated (C14:0, C16:0, C18:0) to anteiso-branched fatty acids (C15:0anteiso, C17:0anteiso, C17:1anteisoΔ9cis). The results show that anaerobic bacteria react with similar mechanisms like aerobic bacteria in order to adapt their membrane to toxic organic solvents. The observed adaptive modifications on the level of membrane fatty acid composition can only be carried out with de novo synthesis of the fatty acids which is strictly related to cell growth. As the growth rates of anaerobic bacteria are generally much lower than in the so far investigated aerobic bacteria, this adaptive response needs more time in anaerobic bacteria. This might be one explanation for the previously observed higher sensitivity of anaerobic bacteria when compared with aerobic ones.
Departments of Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
Ilka
I
Duldhardt
Julia
J
Gaebel
Lukasz
L
Chrzanowski
Ivonne
I
Nijenhuis
Claus
C
Härtig
Frieder
F
Schauer
Hermann J
HJ
Heipieper
D000222:Adaptation, Physiological; D000693:Anaerobiosis; D002462:Cell Membrane / Q000737:chemistry*; D020564:Deltaproteobacteria / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis*; D009930:Organic Chemicals / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism; D020628:Thauera / Q000737:chemistry / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D004785:Environmental Pollutants; D005227:Fatty Acids; D009930:Organic Chemicals; D012997:Solvents
FEMS Microbiol Lett. 2007 Jan;266(2):138-43
17233723
J Lipid Res. 1964 Oct;5:600-8
14221106
Arch Microbiol. 1993;159(4):336-44
8387263
J Bacteriol. 1976 Feb;125(2):670-8
1107328
J Biol Chem. 1994 Mar 18;269(11):8022-8
8132524
Nat Rev Microbiol. 2003 Oct;1(1):35-44
15040178
Science. 2003 Dec 12;302(5652):1967-9
14671304
Appl Environ Microbiol. 1992 Jun;58(6):1847-52
1622260
Microbiol Rev. 1995 Jun;59(2):201-22
7603409
Int J Syst Bacteriol. 1995 Apr;45(2):327-33
7537067
Appl Environ Microbiol. 2008 Apr;74(8):2267-74
18263750
Chemosphere. 2003 Dec;53(8):795-808
14505700
Can J Biochem Physiol. 1959 Aug;37(8):911-7
13671378
Appl Environ Microbiol. 1977 May;33(5):1233-6
327934
Appl Environ Microbiol. 1987 Dec;53(12):2854-61
16347502
Microbiol Rev. 1991 Jun;55(2):288-302
1886522
Arch Microbiol. 1987 Sep;148(3):213-7
3675113
Lipids. 1996 Feb;31(2):129-37
8835399
Appl Environ Microbiol. 1994 Dec;60(12):4440-4
7811084
FEMS Microbiol Lett. 2003 Mar 28;220(2):223-7
12670684
Appl Microbiol Biotechnol. 2007 Dec;77(3):705-11
17876576
Environ Microbiol. 1999 Jun;1(3):191-8
11207738
Proc Natl Acad Sci U S A. 1974 Feb;71(2):522-5
4360948
Science. 2001 Aug 24;293(5534):1444-6
11520973
Prog Lipid Res. 1990;29(3):167-227
2131463
Environ Microbiol. 2003 Nov;5(11):1064-70
14641586
Microbiology (Reading). 2005 Feb;151(Pt 2):607-613
15699209
Appl Environ Microbiol. 1994 Oct;60(10):3752-9
7527204
Environ Sci Technol. 2004 Jan 15;38(2):617-31
14750740
Biochim Biophys Acta. 1982 Dec 8;693(1):195-204
7150588
Appl Environ Microbiol. 1991 Apr;57(4):1139-45
2059037
Appl Microbiol Biotechnol. 2007 Apr;74(5):961-73
17262209
Biochim Biophys Acta. 1996 Oct 29;1286(3):225-45
8982284
Arch Microbiol. 1998 Oct;170(5):361-9
9818355
Environ Microbiol. 2007 Jul;9(7):1658-64
17564601
Curr Opin Microbiol. 2002 Apr;5(2):202-5
11934618