Interaction of gold nanoparticles with Pfu DNA polymerase and effect on polymerase chain reaction.
The interaction of gold nanoparticles with Pfu DNA polymerase has been investigated by a number of biological, optical and electronic spectroscopic techniques. Polymerase chain reaction was performed to show gold nanoparticles' biological effect. Ultraviolet-visible and circular dichroism spectra analysis were applied to character the structure of Pfu DNA polymerase after conjugation with gold nanoparticles. X-ray photoelectron spectroscopy was used to investigate the bond properties of the polymerase-gold nanoparticles complex. The authors demonstrate that gold nanoparticles do not affect the amplification efficiency of polymerase chain reaction using Pfu DNA polymerase, and Pfu DNA polymerase displays no significant changes of the secondary structure upon interaction with gold nanoparticles. The adsorption of Pfu DNA polymerase to gold nanoparticles is mainly through Au-NH(2) bond and electrostatic interaction. These findings may have important implications regarding the safety issue as gold nanoparticles are widely used in biomedical applications.
Xiamen University, College of Materials, Department of Biomaterials, Xiamen, People's Republic of China. sunliping@xmu.edu.cn
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D000327:Adsorption; D002712:Chlorides / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000187:drug effects; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology; D017612:Gold Compounds / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D016133:Polymerase Chain Reaction / Q000379:methods*; D013053:Spectrophotometry
D002712:Chlorides; D017612:Gold Compounds; D006046:Gold; C024568:gold tetrachloride, acid; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase