[Determination of geniposide and croein-1 in different breed of fructus gardeniae].
OBJECTIVE
To determinate geniposide and croein-1 in Fructus Gardeniae from different breeds.


METHODS
RP-HPLC method was adopted. The chromatographic separation was performed on an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 microm, Agilent). The mobile phase was a mixture of acetonitrile-0.1% phosphoric acid aqueous solution in gradient elution. The wavelength of 238 nm and 440 nm was selected to determinate geniposide and crocins simultaneously.


RESULTS
The contents of geniposide and croein-1 in different breeds of Fructus Gardeniae are different.


CONCLUSIONS
This method is simple and repeatable, and could be used for the quality control of Fructus Gardeniae.
Jiangxi University of Traditional Chinese Medicins, Nanchang 330004, China.
Guang-Ming
GM
Luo
Yan
Y
Chen
Xia
X
Li
Ya-Qin
YQ
Yang
Jin-Xiang
JX
Zeng
Bo
B
Wu
Ji-Xiao
JX
Zhu
Yu-Ye
YY
Zhu
D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry* / Q000254:growth & development; D039823:Iridoids / Q000032:analysis*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D039823:Iridoids; C007835:geniposide; D002338:Carotenoids