Anti-arthritic activity of synthesized chondroitin sulfate E hexasaccharide.
The purpose of this study was to investigate the anti-arthritic effects of synthesized chondroitin sulfate E hexasaccharide (sCSE-6, CAS 866407-73-0), using a type II collagen-induced arthritis model in mice. sCSE-6 was administered subcutaneously on a daily basis to type II collagen (CII)-sensitized mice from day 0 to day 55. The severity of arthritis, as well as the immunohistological features of the arthritic mice, were analyzed. sCSE-6 inhibited the course of arthritis and restored the body weight loss of CII-immunized mice. An immunohistological analysis showed that bone/cartilage destruction in the arthritic mice was significantly attenuated by sCSE-6 treatment, with a marginal inhibition of synovial inflammation also observed. The beneficial effect of sCSE-6 on bone destruction, which is the most important factor in preventing arthritis, is particularly noteworthy. In summary, sCSE-6 inhibited arthritis and helped to prevent bone and cartilage destruction in a type II collagen-induced arthritis model in mice. The findings indicated that CSE oligosaccharides might be a novel potential therapeutic tool for rheumatoid arthritis.
Research Administration, Taisho Pharmaceutical Co., Ltd., Saitama, Japan. ta.matsumoto@po.rd.taisho.co.jp
Taro
T
Matsumoto
Tomoyuki
T
Inoue
Akiko
A
Takahashi
Akira
A
Higuchi
Kayo
K
Taniguchi-Morita
Masayuki
M
Miyasaka
Jun-ichi
J
Tamura
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis* / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D002240:Carbohydrate Sequence; D002356:Cartilage / Q000473:pathology; D002809:Chondroitin Sulfates / Q000138:chemical synthesis* / Q000494:pharmacology*; D003907:Dexamethasone / Q000627:therapeutic use; D006614:Hindlimb / Q000473:pathology; D007596:Joints / Q000473:pathology; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009161:Mycobacterium; D021241:Spectrometry, Mass, Electrospray Ionization
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; C501229:chondroitin sulfate E hexasaccharide; D003907:Dexamethasone; D002809:Chondroitin Sulfates