Expressions of lipid oxidation markers, N(ε)-hexanoyl lysine and acrolein in cisplatin-induced nephrotoxicity in rats.
The purpose of this study was to evaluate whether N(ε)-hexanoyl lysine (N(ε)-HEL) and acrolein reflect the severity of cisplatin-induced nephrotoxicity. Immunoexpression of N(ε)-HEL and acrolein in kidneys and their urinary concentration were examined up to day 4 post-cisplatin injection in rats. Cisplatin-induced tubular injury was observed histopathologically on days 2-4 after injection and became more severe time-dependently. On days 2-4, N(ε)-HEL and acrolein were immunostained in the cytoplasm of damaged tubular cells. Their immunostaining intensity and urinary levels increased as tubular injury became more severe. These results suggest that expressions of N(ε)-HEL and acrolein were associated with the pathogenesis of cisplatin-induced nephrotoxicity.
Course of Veterinary Laboratory Medicine, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Tottori 680–8553, Japan. sugiyama@muses.tottori-u.ac.jp
Akihiko
A
Sugiyama
Jing
J
Sun
Masaaki
M
Nishinohara
Yuka
Y
Fujita
Aino
A
Masuda
Tairin
T
Ochi
Takashi
T
Takeuchi
D000171:Acrolein / Q000378:metabolism* / Q000652:urine; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000652:urine; D050356:Lipid Metabolism; D008239:Lysine / Q000378:metabolism / Q000652:urine; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar
D000970:Antineoplastic Agents; C120589:N(epsilon)-hexanoyllysine; D000171:Acrolein; D008239:Lysine; D002945:Cisplatin