Endosulfan affects health variables in adult zebrafish (Danio rerio) and induces alterations in larvae development.
Adult zebrafish (Danio rerio) were exposed to 0 (control), 0.16 or 0.48μg/L of the insecticide, endosulfan, for 28days. Haematology, whole body ions, thiobarbituric acid reactive substances (TBARS), Na(+)K(+)-ATPase, organ histology and reproduction were assessed in adults. The resulting offspring were examined for latent effects on development (heart rate and morphometrics). On day 14, adult fish exposed to 0.16μg/L endosulfan showed significantly lower red blood cell counts than those exposed to 0.48μg/L endosulfan; adult fish exposed to 0.16 ug/L also showed elevated TBARS compared to controls. Both concentrations of endosulfan caused a 4.0 fold increase in Na(+)K(+)-ATPase activity compared to controls (ANOVA, p<0.05). On day 14, the livers of fish exposed to endosulfan had fewer, enlarged hepatocytes, with cell diameters greater than the controls (ANOVA, p<0.05). Morphological alterations in the progeny of fish exposed to endosulfan were observed. Heart beat frequency was significantly lower in larvae from exposed adults to 0.16 μg/L compared to the control (ANOVA, p<0.05). These findings show that sublethal exposure to endosulfan causes adverse sublethal effects in adult D. rerio, and effects on the development of their offspring.
Research Group on Reproduction and Toxicology of Aquatic Organisms (GRITOX), Aquaculture Institute, University of the Llanos, Km 4 vía Puerto López, Villavicencio, Meta, Colombia. ymvelasco@yahoo.com
Yohana M
YM
Velasco-Santamaría
Richard D
RD
Handy
Katherine A
KA
Sloman
D000740:Anemia / Q000139:chemically induced; D000818:Animals; D004726:Endosulfan / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D005880:Gills / Q000187:drug effects / Q000473:pathology; D006339:Heart Rate / Q000187:drug effects; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008141:Lordosis / Q000139:chemically induced; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D018384:Oxidative Stress; D018812:Paternal Exposure / Q000009:adverse effects*; D012098:Reproduction / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013723:Teratogens / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*; D014882:Water-Electrolyte Balance / Q000187:drug effects; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism
D007306:Insecticides; D013723:Teratogens; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; D000254:Sodium-Potassium-Exchanging ATPase; D004726:Endosulfan