Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors.
Recombinant Towne CMV expressing luciferase under the control of CMV-DNA polymerase (POL) or the late pp28 (UL99) promoters were evaluated for potential application in high-throughput screening of anti-viral compounds. POL-and pp28-luciferase displayed maximal expression 48 and 72 hours post infection, respectively. The pp28-luciferase virus achieved a wider dynamic range of luciferase expression (6-7 logs) and was selected for testing of inhibition by five anti-viral compounds. Luciferase expression highly correlated with plaque reduction and real-time PCR. The pp28-luciferase reporter system is rapid, reproducible, and highly sensitive. It may be applied to screening of novel anti-CMV compounds.
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ran
R
He
Gordon
G
Sandford
Gary S
GS
Hayward
William H
WH
Burns
Gary H
GH
Posner
Michael
M
Forman
Ravit
R
Arav-Boger
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000188:drug therapy / Q000821:virology*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000998:Antiviral Agents; D011994:Recombinant Proteins; D008156:Luciferases
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