Short-term changes in the composition of active marine bacterial assemblages in response to diesel oil pollution.
The changes caused by diesel oil pollution in the metabolically active bacterioplankton from an oligotrophic coastal location were analysed in laboratory microcosms (44 l) using 16S ribosomal RNA (16S rRNA) as molecular marker. The aim was to simulate typical hydrocarbon pollution events in a coastal area exploited for seasonal touristic activities. The experiment consisted in addition of low amounts of diesel oil without nutrients to seawater collected at different times (winter and summer). Bacterial diversity was analysed by terminal-restriction fragment length polymorphism (T-RFLP) profiling of 16S rRNAs after reverse transcription polymerase chain reaction (RT-PCR), and by generation of 16S rRNA clone libraries in control and diesel-polluted microcosms. Diesel addition caused a twofold increase in prokaryotic numbers in comparison with controls at the end of the experiment, both in winter and summer microcosms. Bacterioplankton composition, determined by 16S rRNA T-RFLP data, changed rapidly (within 17 h) in response to treatment. The resulting communities were different in microcosms with water collected in summer and winter. A reduction in diversity (Shannon index, calculated on the basis of T-RFLP data) was observed only in summer microcosms. This was due to the rapid increase of phylotypes affiliated to the Oceanospirillaceae, not observed in winter microcosms. After diesel treatment there was a reduction in the number of phylotypes related to SAR11, SAR86 and picocyanobacteria, while phylotypes of the Roseobacter clade, and the OMG group seemed to be favoured. Our results show that diesel pollution alone caused profound effects on the bacterioplankton of oligotrophic seawater, and explained many of the differences in diversity reported previously in pristine and polluted sites in this coastal area.
Microbiologia, Department de Biologia, Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain.
Mariana P
MP
Lanfranconi
Rafael
R
Bosch
Balbina
B
Nogales
D001419:Bacteria / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D005742:Gasoline / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000032:analysis / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000494:pharmacology*; D014877:Water Pollution, Chemical / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005742:Gasoline; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical
FEMS Microbiol Ecol. 2007 Sep;61(3):496-508
17627779
Environ Microbiol. 2005 Jan;7(1):78-87
15643938
FEMS Microbiol Ecol. 2000 Jul 1;33(1):51-59
10922503
Mol Ecol. 2007 Feb;16(4):867-80
17284217
Appl Environ Microbiol. 2006 Sep;72(9):5734-41
16957188
Environ Microbiol. 2007 Aug;9(8):1913-29
17635539
Science. 2005 Aug 19;309(5738):1242-5
16109880
Appl Environ Microbiol. 2004 Jan;70(1):432-40
14711672
FEMS Microbiol Ecol. 2007 Apr;60(1):98-112
17250750
Appl Environ Microbiol. 2009 Dec;75(23):7537-41
19801464
Nucleic Acids Res. 2004 Feb 25;32(4):1363-71
14985472
Appl Environ Microbiol. 1990 Jun;56(6):1919-25
2200342
Environ Microbiol. 2007 Jan;9(1):177-86
17227422
Environ Microbiol. 2007 Oct;9(10):2551-62
17803779
Environ Microbiol. 2002 Mar;4(3):141-7
12000314
Int J Syst Bacteriol. 1995 Jan;45(1):116-23
7857792
Syst Appl Microbiol. 1999 Sep;22(3):434-44
10553296
Syst Appl Microbiol. 2008 Aug;31(3):231-40
18572341
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Arch Microbiol. 2000 Feb;173(2):138-45
10795685
Curr Opin Biotechnol. 2007 Jun;18(3):257-66
17493798
Nat Rev Microbiol. 2006 Mar;4(3):173-82
16489346
Environ Microbiol. 2005 Aug;7(8):1192-9
16011756
Nature. 2002 Dec 19-26;420(6917):806-10
12490947
Appl Environ Microbiol. 2002 Oct;68(10):5017-25
12324352
Microb Ecol. 2005 Jan;49(1):94-103
15883864
Biosci Biotechnol Biochem. 2003 May;67(5):1121-5
12834291
Environ Microbiol. 2007 Jan;9(1):165-76
17227421
Appl Environ Microbiol. 2005 Dec;71(12):7724-36
16332745
Environ Microbiol. 2005 Sep;7(9):1426-41
16104865
Appl Environ Microbiol. 2005 Oct;71(10):5665-77
16204474
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
FEMS Microbiol Ecol. 2005 Nov 1;54(3):375-80
16332335
Appl Environ Microbiol. 2001 Sep;67(9):4374-6
11526051
J Appl Microbiol. 2007 Jan;102(1):184-94
17184334