Development of an in vitro method to estimate lung bioaccessibility of metals from atmospheric particles.
The research presented here was initiated to improve the current knowledge on easily released metals from atmospheric particles. The objectives of this paper were to develop an in vitro method to estimate lung bioaccessibility and to provide quantitative data on metals bioaccessibility. A large set of metals has been investigated (Ba, Cd, Ce, Co, Cu, La, Mn, Mo, Ni, Pb, Rb, Sb and Zn) using two distinct fluids (water and Gamble solution) on four reference materials representing different types of particle sources. Through this study, different parameters such as extraction-time, composition of the leaching solution and solid-to-liquid (S/L) ratios were investigated. The findings obtained for four SRMs suggest that the bioaccessibility is maximized after a 24 h extraction for a range of S/L ratios varying from 1/500 to 1/50,000. We clearly demonstrate that the higher bioaccessibility of metals is obtained with the Gamble solution. Moreover, our results imply that bioaccessibility is speciation and element dependent with percentages varying from 3.3% for Pb to 92.5% for Zn. An estimation of uncertainties of 11% to 30% was obtained for metals bioaccessibility in the four reference materials. In addition, the extraction procedure was validated by performing a mass balance on both soluble and insoluble fractions. This developed method may be used to evaluate the pulmonary bioaccessibility of trace elements present in the atmospheric particles without major artefacts.
Université Lille Nord de France, F-59000, Lille, France. julien.caboche@mines-douai.fr
Caboche
C
Julien
Perdrix
P
Esperanza
Malet
M
Bruno
Laurent Y
LY
Alleman
D001682:Biological Availability; D006801:Humans; D019570:Inhalation Exposure; D008168:Lung / Q000378:metabolism*; D008670:Metals / Q000493:pharmacokinetics*; D052638:Particulate Matter / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D035501:Uncertainty
D008670:Metals; D052638:Particulate Matter