FRET (fluorescence resonance energy transfer) sheds light on transcription.
The complex organization of the transcription machinery has been revealed mainly by biochemical and crystallographic studies. X-ray structures describe RNA polymerases and transcription complexes on an atomic level, but fail to portray their dynamic nature. The use of fluorescence techniques has made it possible to add a new layer of information to our understanding of transcription by providing details about the structural rearrangement of mobile elements and the network of interactions within transcription complexes in solution and in real-time.
RNAP Laboratory, UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK. d.grohmann@ucl.ac.uk
Dina
D
Grohmann
Daniel
D
Klose
Daniel
D
Fielden
Finn
F
Werner
D018360:Crystallography, X-Ray; D012321:DNA-Directed RNA Polymerases / Q000032:analysis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008027:Light; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D014158:Transcription, Genetic
D046912:Multiprotein Complexes; D012321:DNA-Directed RNA Polymerases