Towards biochemical filters with a sigmoidal response to pH changes: buffered biocatalytic signal transduction.
We realize a biochemical filtering process by introducing a buffer in a biocatalytic signal-transduction logic system based on the function of an enzyme, esterase. The input, ethyl butyrate, is converted into butyric acid--the output signal, which in turn is measured by the drop in the pH value. The developed approach offers a versatile "network element" for increasing the complexity of biochemical information processing systems. Evaluation of an optimal regime for quality filtering is accomplished in the framework of a kinetic rate-equation model.
Instituto de Catálisis y Petroleoquímica, CSIC, C/Marie Curie 2, 280409 Madrid, Spain.
Marcos
M
Pita
Vladimir
V
Privman
Mary A
MA
Arugula
Dmitriy
D
Melnikov
Vera
V
Bocharova
Evgeny
E
Katz
D000818:Animals; D055162:Biocatalysis; D002021:Buffers; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid / Q000378:metabolism; D039301:Computers, Molecular; D004950:Esterases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D013552:Swine
D002021:Buffers; D002087:Butyrates; D020148:Butyric Acid; D004950:Esterases; C045572:ethyl butyrate