Scanning tunneling microscopy of cauliflower-like DNA nanostructures synthesised by loop-mediated isothermal amplification.
DNA nanotechnology is a novel approach for synthesis of DNA-based nanostructures. Stem-loops, nanojunctions, sticky-ends and periodic lengths of DNA are the most essential nanostructures in DNA nanofabrications. Loop-mediated isothermal amplification (LAMP) is a powerful technology for repetitive synthesis of double-stranded and cauliflower-like DNAs. The process leads to long and repetitive sequences of DNAs, which are fabricated via loop primers. The authors demonstrate here scanning tunneling micrographs of LAMP-synthesised DNAs deposited on highly ordered pyrolytic graphite. The scans are compared with natural DNAs. Scanning tunneling microscopy (STM) images indicated the creation of periodic long DNAs, stem-looped DNAs and three-way DNA nanojunctions. It is also suggested that such nanomaterials could be promising candidates for use in DNA-based nanodevices.
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran.
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Gill
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Ranjbar
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Saber
D010582:Bacteriophage lambda / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D036103:Nanotechnology / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009690:Nucleic Acid Conformation
D004279:DNA, Viral; D006108:Graphite; D004247:DNA