[Study on the change of angiotensin II expression in rat lung exposure to acrolein].
OBJECTIVE
To investigate the changes of angiotensin II (Ang II) expression in a rat model of mucus hypersecretion induced by acrolein.


METHODS
A rat model of mucous hypersecretion of airway was established by acrolein-inhalation. The AB/PAS stain was performed to evaluate the mucous secretion level. Immunohistochemistry and Western blot were employed to detect the expression of Ang II protein.


RESULTS
Hypersecretion of mucus occurred at 3 weeks and peaked at 6 weeks post exposure (wpe) to acrolein. Increased immunostaining and protein level of Ang II were readily detected in rat lung at 3 wpe and enhanced at 6 wpe.


CONCLUSIONS
The increase of Ang II expression, which associated to pulmonary mucus hypersecretion, might play a role in the lung injury induced by acrolein exposure.
Department of Respiration, the First Affiliated Hospital of Yunnan Province, Kunming Medical College, Kunming 650000, China.
Shan
S
Zhao
Bing
B
Yuan
Qi-qin
QQ
Dan
Sheng-lan
SL
Wang
Bu-liang
BL
Meng
Yun-hui
YH
Zhang
D000171:Acrolein / Q000633:toxicity*; D000804:Angiotensin II / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004781:Environmental Exposure / Q000009:adverse effects; D008168:Lung / Q000378:metabolism*; D008297:Male; D009093:Mucus / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats
D012333:RNA, Messenger; D000804:Angiotensin II; D000171:Acrolein