Genome shuffling of marine derived bacterium Nocardia sp. ALAA 2000 for improved ayamycin production.
Genome shuffling is a recent development in microbiology. The advantage of this technique is that genetic changes can be made in a microorganism without knowing its genetic background. Genome shuffling was applied to the marine derived bacterium Nocardia sp. ALAA 2000 to achieve rapid improvement of ayamycin production. The initial mutant population was generated by treatment with ethyl methane sulfonate (EMS) combined with UV irradiation of the spores, resulting in an improved population (AL/11, AL/136, AL/213 and AL/277) producing tenfold (150 μg/ml) more ayamycin than the original strain. These mutants were used as the starting strains for three rounds of genome shuffling and after each round improved strains were screened and selected based on their ayamycin productivity. The population after three rounds of genome shuffling exhibited an improved ayamycin yield. Strain F3/22 yielded 285 μg/ml of ayamycin, which was 19-fold higher than that of the initial strain and 1.9-fold higher than the mutants used as the starting point for genome shuffling. We evaluated the genetic effect of UV + EMS-mutagenesis and three rounds of genome shuffling on the nucleotide sequence by random amplified polymorphic DNA (RAPD) analysis. Many differences were noticed in mutant and recombinant strains compared to the wild type strain. These differences in RAPD profiles confirmed the presence of genetic variations in the Nocardia genome after mutagenesis and genome shuffling.
Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza, Egypt.
Mervat M A
MM
El-Gendy
Ahmed M A
AM
El-Bondkly
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D032621:DNA Shuffling; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D016680:Genome, Bacterial; D006588:Hexanones / Q000737:chemistry / Q000378:metabolism*; D016296:Mutagenesis; D009154:Mutation; D009578:Nitrobenzenes / Q000737:chemistry / Q000378:metabolism*; D009615:Nocardia / Q000235:genetics / Q000378:metabolism*; D019105:Random Amplified Polymorphic DNA Technique; D014466:Ultraviolet Rays; D014961:X-Ray Diffraction
C561927:1,1-dichloro-4-ethyl-5-(4-nitrophenyl)hexan-2-one; D000900:Anti-Bacterial Agents; D006588:Hexanones; D009578:Nitrobenzenes; D005020:Ethyl Methanesulfonate