[Involvement of guanine nucleotide exchange factor xLARG in epiboly of cells of the animal pole of Xenopus laevis embryos].
The development of multicellular organisms is a complicated coordinated process of the movement of groups of embryonic cells, which is controlled by many regulatory systems. At present little is known about the regulation of the earliest manifestations of the movement in the embryogenesis: epiboly and radial intercalation. The coordinators of these processes may be small GTPases of the Rho family and their activators, the factors of exchange of guanylic nucleotides. It has been shown in this work that the overexpression of the factor of exchange of guanylic nucleotides xLARG in Xenopus laevis embryos leads to an increase in the amount of the active form of xLARG. In addition, an increase in the expression of xLARG disturbs the process of radial intercalation. The data obtained suggest that xLARG is involved in maintaining the xLARG activation level necessary for the occurrence of epiboly.
D O
DO
Kiriukhin
L A
LA
Shustikova
E E
EE
Kopantseva
N N
NN
Luchinskaia
A V
AV
Beliavskiĭ
D000818:Animals; D004625:Embryo, Nonmammalian; D004789:Enzyme Activation; D020662:Guanine Nucleotide Exchange Factors / Q000096:biosynthesis / Q000502:physiology*; D066298:In Vitro Techniques; D064067:Rho Guanine Nucleotide Exchange Factors; D029867:Xenopus Proteins / Q000096:biosynthesis / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
C556283:ARHGEF12 protein, Xenopus; D020662:Guanine Nucleotide Exchange Factors; D064067:Rho Guanine Nucleotide Exchange Factors; D029867:Xenopus Proteins; D020742:rhoA GTP-Binding Protein