Insulin sensitivity is not associated with palmitoleate availability in obese humans.
We evaluated whether insulin resistance in obese people is associated with decreased plasma palmitoleate availability. Palmitoleate content (percentage and absolute concentrations) in FFA and VLDL was measured in obese subjects who were either insulin resistant (IR) or insulin sensitive (IS), based on assessment of multiorgan (skeletal muscle, liver, and adipose tissue) insulin sensitivity by using the hyperinsulinemic-euglycemic clamp procedure in conjunction with infusion of stable isotopically labeled tracers. Plasma palmitoleate concentration and the relative contribution of palmitoleate to total plasma FFA concentration in the IS group (0.018 ± 0.002 mmol/l and 4.4% ± 0.2%, respectively) were not significantly different than values in the IR group (0.023 ± 0.003 mmol/l and 4.4% ± 0.4%, respectively). Plasma VLDL-triglyceride palmitoleate concentration and the proportion of VLDL fatty acids as palmitoleate in the IS group (0.09 ± 0.02 mmol/l and 5.7 ± 0.3%, respectively) were also not significantly different than those in the IR group (0.16 ± 0.04 mmol/l and 5.0% ± 0.4%, respectively). These data demonstrate that decreased palmitoleate in plasma and in VLDL is not associated with insulin resistance in skeletal muscle, liver, or adipose tissue in obese people.
Center for Human Nutrition and Atkins Center of Excellence in Obesity Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Elisa
E
Fabbrini
Faidon
F
Magkos
Xiong
X
Su
Nada A
NA
Abumrad
Nicole
N
Nejedly
Carrie C
CC
Coughlin
Adewole L
AL
Okunade
Bruce W
BW
Patterson
Samuel
S
Klein
D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D005229:Fatty Acids, Monounsaturated / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015309:Glucose Clamp Technique; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000097:blood*; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism
D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; C008757:palmitoleic acid; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase
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