Kainate promotes alterations in neuronal RNA splicing machinery.
Kainate, a glutamate analogue, activates kainate and AMPA receptors inducing strong synaptic activation. Systemic kainate application to rodents results in seizures, neurodegeneration, and neuronal remodeling in the brain. It is therefore used to investigate molecular mechanisms responsible for these conditions. We analyzed proteome alterations in murine primary cortical neurons after 24 h of kainate treatment. Our 2-D gel based proteomics approach revealed 91 protein alterations, some already associated with kainate-induced pathology. In addition, we found a large number of proteins which have not previously been reported to be associated with kainate-induced pathology. Functional classification of altered proteins revealed that they predominantly participate in mRNA splicing and cytoskeleton remodeling.
Institute for Human Genetics, Charité-University Medicine, Berlin, Germany.
Michael
M
Rohe
Grit
G
Nebrich
Oliver
O
Klein
Lei
L
Mao
Claus
C
Zabel
Joachim
J
Klose
Daniela
D
Hartl
D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D007608:Kainic Acid / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D020543:Proteome / Q000032:analysis; D012326:RNA Splicing / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods
D020543:Proteome; D012333:RNA, Messenger; D007608:Kainic Acid