[Surface modification of RGD peptides onto acellularized porcine aortic valve to promote cell adhesion].
OBJECTIVE
To investigate the impact of RGD peptides on cell adhesion to acellularized procine aortic valve.


METHODS
The acellular porcine aorta valve (APAV) was prepared by removing the cells and cellular components from porcine aortic valve using trypsin and hyposmosis TritonX-100. With the help of epoxy chloropropane (EC), the decelluarized valve scaffolds were immobilized with YGRGDSP peptide. MFBs were seeded onto four groups [acellularized value (AV) group, EC group, glutaraldehyde+EC (GE) group and EC+ RGD group or GE+RGD group] of coupled, coated and untreated decelluarized valve scaffolds. Ninhydrin reaction, cell count and fluorescent imaging test were employed to examine the efficiency of cell adhesion.


RESULTS
More cells were attached to the decellularized valve scaffolds when the cells were coupled with RGD peptides compared with the others. The adhesive effect was correlated with the concentration of the RGD peptide and the attaching time.


CONCLUSIONS
With the help of EC, YGRGDSP peptides can be immobilized by covalent bonding. RGD peptides improve cell adhesion to decellularized valve scaffolds.
Department of Cardiothoracic Surgery, the First Affiliated Hospital, Chengdu College of Medicine, Chengdu 610500, China.
Li-ming
LM
Guo
Xiao-fei
XF
Zeng
Rui-dong
RD
Ma
Guan-sheng
GS
Shang
Ming
M
Hao
Ding-hua
DH
Yi
D000818:Animals; D001021:Aortic Valve / Q000166:cytology; D001705:Bioprosthesis; D002448:Cell Adhesion / Q000187:drug effects; D006350:Heart Valve Prosthesis; D009842:Oligopeptides; D011474:Prosthesis Design; D013499:Surface Properties; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid