UF-1000i flow cytometry is an effective screening method for urine specimens.
This study was undertaken to evaluate the UF-1000i™ (UF) flow cytometer to count urine constituents including bacteria. The objective was to screen urine samples and determine what white blood cell (WBC) and/or bacteria screening criteria would minimize the number of specimens cultured yet ensuring that all true positives were cultured. UF screening and culture on CHROMagar™ Orientation (CO) medium were performed on 2496 specimens. Various combinations of WBC/bacterial counts were assessed as screening criteria and correlated with significant growth on CO medium. A bacterial count of ≥20 from UF gave an overall screening sensitivity of 92.6%, allowing 35% of specimens to be screened out and not cultured. The sensitivity was 99.2% and 85.0% for Gram-negative and Gram-positive organisms, respectively, using the same bacterial count. Our study indicated that UF was a simple, rapid, and reliable method for urine screening when the bacterial count of ≥20 was used as the sole screening criterion.
Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, R3E 0J9 Manitoba, Canada.
Kamran
K
Kadkhoda
Kanchana
K
Manickam
Pat
P
Degagne
Pat
P
Sokolowski
Paulette
P
Pang
Nick
N
Kontzie
Michelle
M
Alfa
D000293:Adolescent; D000328:Adult; D000368:Aged; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D058491:Bacterial Load; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D014556:Urine / Q000166:cytology / Q000382:microbiology; D055815:Young Adult