Streamlined purification of plasmid DNA from prokaryotic cultures.
We describe the complete process of AcroPrep Advance Filter Plates for 96 plasmid preparations, starting from prokaryotic culture and ending with high purity DNA. Based on multi-well filtration for bacterial lysate clearance and DNA purification, this method creates a streamlined process for plasmid preparation. Filter plates containing silica-based media can easily be processed by vacuum filtration or centrifuge to yield appreciable quantities of plasmid DNA. Quantitative analyses determine the purified plasmid DNA is consistently of high quality with average OD(260/280;) ratios of 1.97. Overall, plasmid yields offer more pure DNA for downstream applications, such as sequencing and cloning. This streamlined method of using AcroPrep Advance Filter Plates allows for manual, semi-automated or fully-automated processing.
Pall Life Sciences.
Laura
L
Pueschel
Hongshan
H
Li
Matthew
M
Hymes
D004247:DNA / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D005821:Genetic Techniques; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification*; D011387:Prokaryotic Cells / Q000737:chemistry*
D004247:DNA