Construction of recombinant mercury resistant Acidithiobacillus caldus.
A mercury-resistant plasmid of pTMJ212 which was able to shuttle between Acidithiobacillus caldus and Escherichia coli was constructed by inserting the mercury resistant determinants, the mer operon of Acidithiobacillus ferrooxidans, into the IncQ plasmid of pJRD215. pTMJ212 was transferred from Escherichia coli into Acidithiobacillus caldus through conjugation. Furthermore, pTMJ212 was transferred back from Acidithiobacillus caldus into Escherichia coli, thereby confirming the initial transfer of pTMJ212 from Escherichia coli to Acidithiobacillus caldus. Compared to the control, the cell growth of the recombinant Acidithiobacillus caldus increased markedly under mercury (Hg(2+)) stress especially at Hg(2+) concentrations ranging from 2.0 to 4.5 μg/ml.
State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China.
Dandan
D
Chen
Jianqun
J
Lin
Yuanyuan
Y
Che
Xiangmei
X
Liu
Jianqiang
J
Lin
D042763:Acidithiobacillus / Q000187:drug effects* / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D003227:Conjugation, Genetic; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics; D022761:Gene Transfer, Horizontal; D005822:Genetic Vectors; D008628:Mercury / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D010957:Plasmids
D000900:Anti-Bacterial Agents; D008628:Mercury