Characterization of the terminal activation step catalyzed by oxygenase CmmOIV of the chromomycin biosynthetic pathway from Streptomyces griseus.
Inactivation and initial interrogation of key oxygenase CmmOIV of the biosynthetic pathway of chromomycin A(3) in Streptomyces griseus ssp. griseus revealed that a completely methylated and acetylated prechromomycin is the preferred substrate of this enzyme. This suggests that the three sugar decoration reactions, two O-acetylations and an O-methylation, which were previously believed to occur as the final steps of chromomycin A(3) biosynthesis, indeed take place prior to the CmmOIV reaction. Upon inactivation of CmmOIV, four new compounds accumulated; the fully decorated prechromomycin and its incompletely acetylated precursor along with a diketoprechromomycin-type compound were fully characterized and assayed with CmmOIV.
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596, USA.
Mary A
MA
Bosserman
Ana B
AB
Flórez
Khaled A
KA
Shaaban
Alfredo F
AF
Braña
Jose A
JA
Salas
Carmen
C
Méndez
Jürgen
J
Rohr
D055162:Biocatalysis; D014128:Chromomycin A3 / Q000096:biosynthesis*; D004789:Enzyme Activation; D007700:Kinetics; D009154:Mutation; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D013305:Streptomyces griseus / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D014128:Chromomycin A3; D010105:Oxygenases
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