Lipooligosaccharide of Campylobacter jejuni: similarity with multiple types of mammalian glycans beyond gangliosides.
Campylobacter jejuni is well known for synthesizing ganglioside mimics within the glycan component of its lipooligosaccharide (LOS), which have been implicated in triggering Guillain-Barré syndrome. We now confirm that this pathogen is capable of synthesizing a much broader spectrum of host glycolipid/glycoprotein mimics within its LOS. P blood group and paragloboside (lacto-N-neotetraose) antigen mimicry is exhibited by RM1221, a strain isolated from a poultry source. RM1503, a gastroenteritis-associated strain, expresses lacto-N-biose and sialyl-Lewis c units, the latter known as the pancreatic tumor-associated antigen, DU-PAN-2 (or LSTa). C. jejuni GC149, a Guillain-Barré syndrome-associated strain, expresses an unusual sialic acid-containing hybrid oligosaccharide with similarity to both ganglio and Pk antigens and can, through phase variation of its LOS biosynthesis genes, display GT1a or GD3 ganglioside mimics. We show that the sialyltransferase CstII and the galactosyltransferase CgtD are involved in the synthesis of multiple mimic types, with LOS structural diversity achieved through evolving allelic substrate specificity.
Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada.
R Scott
RS
Houliston
Evgeny
E
Vinogradov
Monika
M
Dzieciatkowska
Jianjun
J
Li
Frank
F
St Michael
Marie-France
MF
Karwaski
Denis
D
Brochu
Harold C
HC
Jarrell
Craig T
CT
Parker
Nobuhiro
N
Yuki
Robert E
RE
Mandrell
Michel
M
Gilbert
D001426:Bacterial Proteins / Q000378:metabolism; D016123:Campylobacter jejuni / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012799:Sialyltransferases / Q000378:metabolism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D005732:Gangliosides; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D005700:Galactosyltransferases; C045097:UDP-galactose-lactosylceramide alpha 1-4-galactosyltransferase; D012799:Sialyltransferases
Eur J Biochem. 1994 May 15;222(1):183-94
7515346
Glycobiology. 2007 Dec;17(12):1333-43
17766267
J Bacteriol. 2008 Aug;190(16):5681-9
18556784
J Biol Chem. 2000 Feb 11;275(6):3896-906
10660542
Eur J Biochem. 1993 May 1;213(3):1017-27
8240486
Immunobiology. 1993 Apr;187(3-5):382-402
8330904
Glycobiology. 2009 Feb;19(2):153-9
18955372
J Exp Med. 1993 Nov 1;178(5):1771-5
8228822
Eur J Biochem. 1993 Jun 15;214(3):703-10
7686488
Biochemistry. 1996 Feb 20;35(7):2489-97
8652593
J Biol Chem. 2006 Apr 28;281(17):11480-6
16481326
J Biol Chem. 2002 Jan 4;277(1):327-37
11689567
J Infect Dis. 2006 Feb 15;193(4):547-55
16425134
Glycobiology. 1995 Oct;5(7):683-8
8608270
Chem Rev. 2000 Dec 13;100(12):4589-614
11749359
Mol Microbiol. 2000 Mar;35(5):1120-34
10712693
FEMS Immunol Med Microbiol. 1996 Dec 1;16(2):105-15
8988391
Methods Mol Biol. 2003;213:263-74
12619996
Curr Opin Neurol. 2008 Oct;21(5):540-6
18769247
Infect Immun. 1992 Jul;60(7):3017-20
1612771
Mol Microbiol. 2000 Feb;35(3):529-41
10672176
J Clin Invest. 2004 Dec;114(11):1659-65
15578098
J Clin Microbiol. 2005 Jun;43(6):2771-81
15956396
Eur J Biochem. 1993 May 1;213(3):1029-37
8504799
Biochemistry. 1994 Jan 11;33(1):241-9
8286348
Biochemistry. 2007 Dec 18;46(50):14704-14
18034462
Methods Enzymol. 1994;236:231-54
7968613
Mass Spectrom Rev. 2007 Jan-Feb;26(1):35-50
16967446
Carbohydr Res. 1983 Sep 16;121:233-41
6421484
Methods Mol Biol. 2010;600:51-65
19882120