Multiple HIV-1 infections with evidence of recombination in heterosexual partnerships in a low risk Rural Clinical Cohort in Uganda.
We report on the frequency of multiple infections, generation of recombinants and consequences on disease progression in 35 HIV-1 infected individuals from 7 monogamous and 6 polygamous partnerships within a Rural Clinical Cohort in Uganda. The env-C2V3, gag-p24 and pol-IN genes were sequenced. Single genome amplified half genome sequences were used to map recombination breakpoints. Three participants were dually infected with subtypes A and D, one case with subtype A and A/D recombinant and the fifth with 2 phylogenetically distinct A/D recombinants. Occurrence of A/D recombination was observed in two multiple infected individuals. Rate of late stage WHO events using Cox regression was 3 times greater amongst multiple infected compared to singly infected individuals (hazard ratio 3.35; 95% CI 1.09, 10.3; p=0.049). We have shown that polygamous relationships involving subtype discordant partnerships was a major contributor of multiple infections with generation of inter subtype recombinants in our cohort.
MRC/UVRI Uganda Research Unit on AIDS, P.O. Box 49 Entebbe, Uganda.
Deogratius
D
Ssemwanga
Frederick
F
Lyagoba
Nicaise
N
Ndembi
Billy N
BN
Mayanja
Natasha
N
Larke
Shuyi
S
Wang
Joshua
J
Baalwa
Carolyn
C
Williamson
Heiner
H
Grosskurth
Pontiano
P
Kaleebu
D000328:Adult; D000554:Ambulatory Care Facilities; D016000:Cluster Analysis; D015331:Cohort Studies; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D020010:Heterosexuality; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D012424:Rural Population; D017422:Sequence Analysis, DNA; D014454:Uganda; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus
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