In vivo antigenotoxicity of baccharin, an important constituent of Baccharis dracunculifolia DC (Asteraceae).
Baccharin (3-prenyl-4-(dihydrocinnamoyloxy)cinnamic acid) is an important chemical compound isolated from the aerial parts of Baccharis dracunculifolia DC (Asteraceae), a native plant of South America, and the most important plant source of Brazilian green propolis. The present study was designed to investigate the ability of baccharin to modulate the genotoxic effects induced by doxorubicin and methyl methanesulphonate in male Swiss mice using the micronucleus and comet assays, respectively. The different doses of baccharin [0.12, 0.24 and 0.48 mg/kg body-weight (b.w.)] were administered simultaneously to doxorubicin (micronucleus test; 15 mg/kg b.w.) and to methyl methanesulphonate (comet assay; 40 mg/kg b.w.). The results showed a significant decrease in the frequency of micronucleated polychromatic erythrocytes in animals treated with baccharin and doxorubicin compared to animals that received only doxorubicin. This reduction ranged from 39.8% to 50.7% in the micronucleus test. The extent of DNA damage in liver cells was significantly lower in animals treated with different concentrations of baccharin combined with methyl methanesulphonate in comparison with the damage observed for animals treated only with methyl methanesulphonate. These differences resulted in a significant reduction in the extent of DNA damage, which ranged from 47.8% to 60.6%.
University of Franca, Franca, São Paulo, Brazil Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Pollyanna F
PF
Oliveira
Moacir A B
MA
Monteiro Neto
Luís F
LF
Leandro
Jairo K
JK
Bastos
Ademar A
AA
da Silva Filho
Denise C
DC
Tavares
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D031175:Baccharis / Q000737:chemistry; D001938:Brazil; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D035261:Plant Components, Aerial; D014255:Trichothecenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D016587:Antimutagenic Agents; D018906:Antineoplastic Agents, Alkylating; D009153:Mutagens; D014255:Trichothecenes; D004317:Doxorubicin; D008741:Methyl Methanesulfonate; C012764:baccharin