Fast voice-coil scanning optical-resolution photoacoustic microscopy.
We developed a photoacoustic imaging system that has real-time imaging capability with optical resolution. The imaging system is capable of scanning at 20 Hz over a 9 mm range and up to 40 Hz over a 1 mm scanning range. A focused laser beam provides a lateral resolution of 3.4 μm as measured in an optically nonscattering medium. Flows of micrometer-sized carbon particles or whole blood in a silicone tube and individual red blood cells (RBCs) in mouse ear capillaries were also imaged in real time, demonstrating the capability to image highly dynamic processes in vivo at a micrometer-scale resolution.
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130-4899, USA.
Lidai
L
Wang
Konstantin
K
Maslov
Junjie
J
Yao
Bin
B
Rao
Lihong V
LV
Wang
D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D002196:Capillaries / Q000166:cytology; D002465:Cell Movement; D004423:Ear / Q000098:blood supply; D004912:Erythrocytes / Q000166:cytology; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D055620:Optical Phenomena; D013997:Time Factors; D014054:Tomography / Q000295:instrumentation*
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