Generation of a specific polyclonal antibody with high affinity to atorvastatin and its employment in the development of ELISA for determination of atorvastatin in plasma.
For the first time, a polyclonal antibody with high affinity to atorvastatin (ATR) was generated. The high specificity of the antibody for ATR among its structural analogues and co-administered therapeutic agents was proved. The antibody was employed in the development of enzyme-linked immunosorbent assay for quantitation of ATR in plasma. The assay was validated over a working range of 0.2-5 ng/mL. The intra- and interassay precisions were satisfactory; the coefficients of variations were ≤5%. The accuracy of the method was proved as the mean recovery was 96.4 ± 4.3%. The assay can be used in therapeutic monitoring and pharmacokinetic studies for ATR.
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. idarwish@ksu.edu.sa
Ibrahim A
IA
Darwish
Abdul-Rahman M
AR
Al-Obaid
Hamoud A
HA
Al-Malaq
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000069059:Atorvastatin; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000276:immunology; D006538:Heptanoic Acids / Q000097:blood* / Q000276:immunology* / Q000493:pharmacokinetics; D011758:Pyrroles / Q000097:blood* / Q000276:immunology* / Q000493:pharmacokinetics; D011817:Rabbits; D015203:Reproducibility of Results
D006241:Haptens; D006538:Heptanoic Acids; D011758:Pyrroles; D000069059:Atorvastatin