Gene expression and promoter region polymorphisms of interleukin-10 in meningitis patients.
Meningitis is an inflammatory disease caused by bacteria, fungi, and viruses with various clinical symptoms. Interleukin-10 (IL-10) levels have been shown to be increased in blood or cerebrospinal fluid of patients with meningitis, but the association of IL-10 gene promoter polymorphisms or gene expression with meningitis has not been evaluated. IL-10 gene promoter polymorphisms A-592C, T-819C, and A-1082G in 61 patients with meningitis and 64 healthy controls were determined by real-time polymerase chain reaction analysis. mRNA from blood and cerebrospinal fluid samples was extracted, and real-time polymerase chain reaction was performed for IL-10 gene expression. No statistically significant differences were found in the allele and genotypic frequencies between patients and control subjects. Expression of IL-10 in meningitis at mRNA levels was detected in the infiltrating leukocytes. IL-10 gene expression in blood from patients was significantly higher than the control group. Our results suggest that there was no association between promoter polymorphisms of IL-10 and meningitis, but a significant increase of IL-10 gene expression was present in patients with meningitis.
Department of Medical Biology, University of Gaziantep, Gaziantep, Turkey. oztuzcu@gantep.edu.tr
Serdar
S
Oztuzcu
Ecir Ali
EA
Cakmak
Ercan
E
Sivasli
Mustafa
M
Namiduru
Mesut
M
Parlak
Mehri
M
Iğci
Yusuf Ziya
YZ
Iğci
Abdullah T
AT
Demiryürek
Beyhan
B
Cengiz
Bülent
B
Gogebakan
Ahmet
A
Arslan
Mehmet Yavuz
MY
Coşkun
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics; D008297:Male; D016920:Meningitis, Bacterial / Q000276:immunology*; D008587:Meningitis, Viral / Q000276:immunology*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
D012333:RNA, Messenger; D016753:Interleukin-10