Broccoli ( Brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters.
This study investigated the effects of broccoli sprouts (BS) on sterol and lipid homeostasis in Syrian hamsters with dietary-induced hypercholesterolemia. Treatments included freeze-dried BS containing 2 or 20 μmol of glucoraphanine (BSX, BS10X), glucoraphanine-rich BS extract (GRE), sulforaphane-rich BS extract (SFE), and simvastatin. Each experimental diet was offered to eight animals (male and female) for 7 weeks. Hepatic cholesterol was reduced by BS10X and SFE treatments in all animals. This correlated with a down-regulation of gene expression of sterol regulatory element-binding proteins (SREBP-1 and -2) and fatty acid synthase (FAS) caused by GRE and SFE diets. BS10X caused changes in gene expression in a gender-specific manner; additionally, it increased coprostanol excretion in females. With the same concentration of glucoraphanin, consumption of broccoli sprouts (BS10X) had more marked effects on cholesterol homeostasis than GRE; this finding reinforces the importance of the matrix effects on the bioactivity of functional ingredients.
Departamento de Biotecnología e Ingeniería de Alimentos, Tecnológico de Monterrey, Avenida Eugenio Garza Sada 2501 Sur, CP 64849, Monterrey, NL, Mexico.
Laura N
LN
Rodríguez-Cantú
Janet A
JA
Gutiérrez-Uribe
Jennifer
J
Arriola-Vucovich
Rocio I
RI
Díaz-De La Garza
Jed W
JW
Fahey
Sergio O
SO
Serna-Saldivar
D000818:Animals; D000924:Anticholesteremic Agents; D001937:Brassica / Q000737:chemistry*; D002784:Cholesterol / Q000378:metabolism; D006224:Cricetinae; D064429:Fatty Acid Synthases / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005961:Glucosinolates / Q000032:analysis*; D006706:Homeostasis / Q000187:drug effects; D017879:Isothiocyanates / Q000032:analysis*; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D008647:Mesocricetus; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018520:Plant Shoots / Q000737:chemistry; D012737:Sex Factors; D051779:Sterol Regulatory Element Binding Proteins / Q000235:genetics
D000924:Anticholesteremic Agents; D005961:Glucosinolates; D017879:Isothiocyanates; D010936:Plant Extracts; D051779:Sterol Regulatory Element Binding Proteins; D002784:Cholesterol; D064429:Fatty Acid Synthases