Development and characterization of Thy*IκBα-SI transgenic mice.
We developed and characterized a new transgenic model where NF-κB activity is inhibited only in mature neurons. Transgenic mouse strain Thy*IκBα-SI was created using trans-dominant super inhibitor NF-κB (IκBα-SI), which is a multimutant form of IκBα inhibitory protein cloned into specific neutral Thy-1.2 cassette. Detailed molecular analysis showed that the transgene and its product (IκBα-SI protein) are expressed in the nervous system of transgenic mice. In situ hybridization showed that Thy*IκBα-SI in the nervous system is expressed exclusively in neurons. The developed model provides wide opportunities for studying functional role of NF-κB in mature neurons in the central and peripheral nervous system in vivo.
Department of Histology and Embryology, Pediatric Faculty, Russian State Medical University, Moscow, Russia. sguschin@mail.ru
S V
SV
Gushchina
G
G
Michael
O V
OV
Volkova
C B
CB
Magoulas
D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D016254:Mutagenesis, Insertional; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009474:Neurons / Q000378:metabolism*; D018800:Thy-1 Antigens
D017931:DNA Primers; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606205:Nfkbia protein, mouse; D018800:Thy-1 Antigens; D000072000:NF-KappaB Inhibitor alpha