RNAi screening for fat regulatory genes with SRS microscopy.
Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.
Department of Molecular and Human Genetics and Huffington Center on Aging, Baylor College of Medicine, Houston, Texas, USA.
Meng C
MC
Wang
Wei
W
Min
Christian W
CW
Freudiger
Gary
G
Ruvkun
X Sunney
XS
Xie
D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005223:Fats / Q000032:analysis* / Q000378:metabolism; D005786:Gene Expression Regulation; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D034622:RNA Interference; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D005223:Fats
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