Developmental expression changes of the genes involved in IGFI signaling pathway in longissimus dorsi muscle of Tongcheng and Yorkshire pigs during postnatal growth.
IGFI signaling pathway is sufficient to regulate myofibre hypertrophy postnatally, which is associated with muscle mass in economically livestock. In the present study, we drafted the developmental expression pattern of eight genes implicated in IGFI system across six stages of postnatal myofibre growth in Yorkshire and Tongcheng pigs. The results indicated that GRB2 may contribute to increased DNA content in postnatal myofibre hypertrophy via GRB2-Ras-Raf-MEK-ERK sub-pathway; INSR, PDK1, IRS1 and eIF4E may contribute to high growth rate via stimulating the rate of protein synthesis and inhibiting the rate of protein degradation. In addition, the results suggested 60 days maybe a very important stage in postnatal myofibre growth. Moreover, higher mRNA level of IRS1 and GLUT4 maybe associated with inferior meat quality in Yorkshire compared to Tongcheng pig. Therefore, IGFI signaling pathway regulates myofibre hypertrophy postnatally via complicated signal effectors, which may have negative impact on meat quality simultaneously.
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Wuhan, People's Republic of China. qingzhenjiao@yahoo.com.cn
Qingzhen
Q
Jiao
Minggang
M
Lei
Linjie
L
Wang
Xiaoyu
X
Mo
Jing
J
Lan
Yuanzhu
Y
Xiong
D000818:Animals; D000831:Animals, Newborn; D001947:Breeding; D002681:China; D018507:Gene Expression Regulation, Developmental; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development*
D007334:Insulin-Like Growth Factor I
J Biol Chem. 2005 May 13;280(19):19426-35
15764603
J Investig Med. 2005 Apr;53(3):135-42
15921033
Am J Physiol. 1990 Jul;259(1 Pt 1):E89-95
2372054
Am J Physiol. 1991 Mar;260(3 Pt 1):C475-84
2003574
Physiology (Bethesda). 2005 Aug;20:271-84
16024515
Mol Biol Rep. 2009 Sep;36(7):2003-10
19051057
BMC Mol Biol. 2007 Aug 15;8:67
17697375
J Anim Sci. 1981 May;52(5):1014-8
7240043
J Anim Sci. 2005 Sep;83(9):2075-86
16100062
Domest Anim Endocrinol. 2004 Oct;27(3):219-40
15451071
J Anim Sci. 1993 Dec;71(12):3339-43
8294285
FASEB J. 2008 Aug;22(8):2990-3000
18417546
Nat Cell Biol. 2001 Nov;3(11):1014-9
11715023
Am J Physiol Cell Physiol. 2002 Apr;282(4):C899-906
11880278
Genes Immun. 2005 Jun;6(4):279-84
15815687
Genome Biol. 2007;8(6):R115
17573972
J Mol Endocrinol. 2005 Jun;34(3):597-601
15956331
Trends Mol Med. 2003 Aug;9(8):344-50
12928036
Methods. 2001 Dec;25(4):402-8
11846609