[Intensification of surfactant synthesis in Rhodococcus erythropolis EK-1 cultivated on hexadecane].
Activity of key enzymes of n-alkane metabolism was determined in cells of Rhodococcus erythropolis EK-1, a surfactant producer grown on n-hexadecane. Potassium cations were found to inhibit alkane hydroxylase and NADP(+)-dependent aldehyde dehydrogenase, while sodium cations were found to activate these enzymes. Decreased potassium concentration (to 1 mM), increased sodium concentration (to 35 mM), and addition of 36 micromol/l Fe(II), required for alkane hydroxylase activity, resulted in increased activity of the enzymes of n-hexadecane metabolism and in a fourfold increase of surfactant synthesis. A 1.5-1.7-fold increase in surfactant concentration after addition of 0.2% fumarate (gluconeogenesis precursor) and 0.1% citrate (lipid synthesis regulator) to the medium with n-hexadecane results from enhanced synthesis of trehalose mycolates, as evidenced by a 3-5-fold increase in phosphoenolpyruvate synthetase and trehalose phosphate synthase, respectively.
T P
TP
Pirog
T A
TA
Shevchuk
Iu A
IuA
Klimenko
D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors; D000473:Alkanes / Q000378:metabolism*; D002412:Cations / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003470:Culture Media; D042926:Cytochrome P-450 CYP4A / Q000037:antagonists & inhibitors; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D005964:Glucosyltransferases / Q000378:metabolism; D017866:Phosphotransferases (Paired Acceptors) / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
D000473:Alkanes; D002412:Cations; D003470:Culture Media; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D005650:Fumarates; D013501:Surface-Active Agents; D019343:Citric Acid; D042926:Cytochrome P-450 CYP4A; D000444:Aldehyde Dehydrogenase; D005964:Glucosyltransferases; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; C007932:n-hexadecane; D011188:Potassium