Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.
Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany.
Christian
C
Conrad
Annelie
A
Wünsche
Tze Heng
TH
Tan
Jutta
J
Bulkescher
Frank
F
Sieckmann
Fatima
F
Verissimo
Arthur
A
Edelstein
Thomas
T
Walter
Urban
U
Liebel
Rainer
R
Pepperkok
Jan
J
Ellenberg
D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012984:Software; D049490:Systems Biology / Q000379:methods*
D002868:Chromosomal Proteins, Non-Histone; C485184:enhanced green fluorescent protein; D000090266:Chromobox Protein Homolog 5; D049452:Green Fluorescent Proteins
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