PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss.
The iol cluster (consisting of genes involved in myo-inositol utilization) was investigated in Lactobacillus casei strains isolated from koumiss. Ten strains were tested for the presence of iol cluster by PCR screening; three strains encoded this cluster. Full-sequencing procedure was conducted; the iol cluster was identical to that of L. casei BL23 (GenBank access. no. FM177140) except for an upstream transposase. The iol cluster is not a common feature for L. casei strains isolated from koumiss.
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, School of Food Science and Engineering, Inner Mongolia Agricultural University, 010018 Huhhot, China.
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D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D006736:Horses; D007780:Lacticaseibacillus casei / Q000235:genetics* / Q000302:isolation & purification; D008403:Mass Screening / Q000379:methods; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
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