Study of the efficiency of transplantation of human neural stem cells to rats with spinal trauma: the use of functional load tests and BBB test.
Human ensheating neural stem cells of the olfactory epithelium were transplanted to adult male rats immediately after contusion trauma of the spinal cord at T9 level rostrally and caudally to the injury. Voluntary movements (by a 21-point BBB scale), rota-rod performance, and walking along a narrowing beam were monitored weekly over 60 days. In rats receiving cell transplantation, the mean BBB score significantly increased by 11% by the end of the experiment. The mean parameters of load tests also regularly surpassed the corresponding parameters in controls. The efficiency of transplantation (percent of animals with motor function recovery parameters surpassing the corresponding mean values in the control groups) was 62% by the state of voluntary motions, 37% by the rota-rod test, and 32% by the narrowing beam test. Morphometry revealed considerable shrinking of the zone of traumatic damage in the spinal cord and activation of posttraumatic remyelination in animals receiving transplantation of human neural stem cells.
V. P. Serbskii State Research Center of Forensic and Social Psychiatry, Moscow, Russia. lebedsv@yandex.ru
S V
SV
Lebedev
A V
AV
Karasev
V P
VP
Chekhonin
E A
EA
Savchenko
I V
IV
Viktorov
Yu A
YA
Chelyshev
G F
GF
Shaimardanova
D000818:Animals; D006801:Humans; D008297:Male; D009043:Motor Activity / Q000502:physiology; D058953:Neural Stem Cells / Q000637:transplantation*; D009831:Olfactory Mucosa / Q000166:cytology; D051381:Rats; D045442:Rotarod Performance Test; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000628:therapy*; D018709:Statistics, Nonparametric; D014183:Transplantation, Heterologous / Q000379:methods*; D016896:Treatment Outcome