Catheterization of intestinal loops in ruminants does not adversely affect loop function.
Catheterized intestinal loops may be a valuable model to elucidate key components of the host response to various treatments within the small intestine of ruminants. We examined whether catheterizing ileal loops in sheep affected the overall health of animals and intestinal function, whether a bacterial treatment could be introduced into the loops through the catheters, and whether broad-spectrum antibiotics could sterilize the loops. Escherichia coli cells transformed to express the GFP gene were introduced readily into the loops through the catheters, and GFP E. coli cells were localized within the injected loops. Catheterized loops, interspaces, and intact ileum exhibited no abnormalities in tissue appearance or electrical resistance. Expression of the IFNγ, IL1α, IL4, IL6, IL12p40, IL18, TGFβ1, and TNFα cytokine genes did not differ significantly among the intact ileum, catheterized loops, and interspaces, nor did the expression of the gene for inducible nitric oxide synthase. Broad-spectrum antibiotics administered during surgery did not sterilize the loops or interspaces and did not substantively change the composition of the microbiota. However, antibiotics reduced the overall number of bacterial cells within the loop and the relative abundance of community constituents. We concluded that catheterization of intestinal loops did not adversely affect health or loop function in sheep. Furthermore, allowing animals to recover fully from surgery and to clear pharmaceuticals will remove any confounding effects due to these factors, making catheterized intestinal loops a feasible model for studying host responses in ruminants.
Agriculture and Agri-Food Canada, Lethbridge, Canada. Douglas.Inglis@agr.gc.ca
G Douglas
GD
Inglis
John P
JP
Kastelic
Richard R E
RR
Uwiera
D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000494:pharmacology; D002404:Catheterization / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D049452:Green Fluorescent Proteins / Q000032:analysis; D007082:Ileum / Q000382:microbiology / Q000502:physiology / Q000601:surgery*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012756:Sheep / Q000601:surgery*
D000900:Anti-Bacterial Agents; D016207:Cytokines; D049452:Green Fluorescent Proteins; D052247:Nitric Oxide Synthase Type II
Dig Dis Sci. 2008 Jun;53(6):1437-42
17987386
Am J Physiol Gastrointest Liver Physiol. 2007 Feb;292(2):G518-25
17023554
Immunology. 1992 Apr;75(4):601-7
1592435
J Gen Virol. 2007 Oct;88(Pt 10):2749-2761
17872528
Appl Environ Microbiol. 2010 Feb;76(4):1110-9
20023098
BMC Bioinformatics. 2009 Jun 06;10:171
19500385
Curr Opin Immunol. 2009 Apr;21(2):153-60
19375293
Cytokine Growth Factor Rev. 2006 Dec;17(6):451-61
17045835
Appl Environ Microbiol. 2003 Aug;69(8):4915-26
12902287
J Microbiol Methods. 2009 Aug;78(2):175-80
19463863
Exp Physiol. 2006 May;91(3):539-50
16452124
Cytokine. 2005 Jul 7;31(1):72-86
15882946
Methods Mol Biol. 2004;268:491-502
15156063
Appl Environ Microbiol. 2009 Sep;75(18):5779-86
19633120
Cytokine Growth Factor Rev. 2002 Aug-Oct;13(4-5):323-40
12220547
Mol Biol Evol. 1996 Mar;13(3):451-61
8742634
Appl Environ Microbiol. 2009 Jun;75(11):3502-12
19363080
J Infect Dis. 2009 Feb 1;199(3):350-4
19133807
Vet Immunol Immunopathol. 2004 Jan;97(1-2):53-63
14700537
J Immunol Methods. 2001 Oct 1;256(1-2):19-33
11516752
BMC Bioinformatics. 2006 Feb 22;7:85
16504059
Vet Res. 2009 Jan-Feb;40(1):5
18922229
J Cyst Fibros. 2004 Aug;3 Suppl 2:123-6
15463943
J Surg Res. 2002 Feb;102(2):85-94
11796003
Vet Pathol. 2002 Mar;39(2):200-15
12009058
Lab Invest. 1991 Dec;65(6):719-31
1753715
Gastroenterology. 2003 Aug;125(2):413-20
12891543
J Vis Exp. 2009 Jun 11;(28):
19521337
Infect Immun. 2004 Mar;72(3):1409-22
14977946
Biol Reprod. 2004 Feb;70(2):473-80
14561657
J Vet Med B Infect Dis Vet Public Health. 2002 Mar;49(2):105-10
12002419
J Pharm Sci. 1992 Jul;81(7):640-5
1403696
Comp Biochem Physiol A Mol Integr Physiol. 2004 Aug;138(4):475-83
15369837
Gastroenterology. 2009 May;136(6):2015-31
19462507
J Anim Sci. 2006 Oct;84(10):2725-34
16971574
Eur J Immunol. 1993 Jun;23(6):1314-21
8500527