FRET-based ratiometric detection system for mercury ions in water with polymeric particles as scaffolds.
Mercury pollution is a global problem, and the development of stable and sensitive fluorescent probes for mercury ions in the water phase has long been sought. In this work, a novel fluorescence resonance energy transfer (FRET)-based ratiometric sensor for detecting Hg(2+) in pure water was demonstrated. Polymeric nanoparticles prepared by miniemulsion polymerization of methyl methacrylate and acrylic acid were used as the scaffold for the FRET-based sensor. A hydrophobic fluorescent dye nitrobenzoxadiazolyl derivative (NBD) was embedded in the nanoparticles during the polymerization and used as the donor. A spirolactam rhodamine derivative SRHB-NH(2) was synthesized and then covalently linked onto the particle surface and used as an ion recognition element. The presence of Hg(2+) in the water dispersion of nanoparticles induced the ring-opening reaction of the spirolactam rhodamine moieties and led to the occurrence of the FRET process, affording the nanoparticle system a ratiometric sensor for Hg(2+). The nanoparticle sensor can selectively detect the Hg(2+) in water with the detection limit of 100 nM (ca. 20 ppb). It has been found that the FRET-based system with smaller nanoparticles as the scaffold exhibited higher energy transfer efficiency and was more preferred for the accurate ratiometric detection. Moreover, the FRET-based sensor was applicable in a relatively wide pH range (pH 4-8) in water; thus, this approach may provide a new strategy for ratiometric detection of analytes in environmental and biological applications.
College of Materials Science & Engineering, South China University of Technology, Guangzhou, China.
Chao
C
Ma
Fang
F
Zeng
Lifang
L
Huang
Shuizhu
S
Wu
D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014874:Water Pollutants, Chemical / Q000032:analysis*
D005456:Fluorescent Dyes; D012235:Rhodamines; D014874:Water Pollutants, Chemical; D008628:Mercury