Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis.
Four biotinylated tri and tetrasaccharide fragments of plant and invertebrate N-glycans were synthesized using methyl tert-butyl phenyl (MBP) thioglycosides donors in order to evaluate their involvement in cross-allergies as cross-reactive carbohydrate determinants (CCDs). Various levels of reactivity to anti-bee and anti-HRP antibodies and with sera from allergic patients were observed when the conjugates were coated on streptavidin microplates. The results showed the potential utility of these xylosylated and fucosylated oligosaccharide fragments in determining CCD antibody epitopes.
Ecole Normale Supérieure, Département de Chimie, Université Paris 6, UMR CNRS 7203, 24 rue Lhomond, 75005 Paris, France.
Mayeul
M
Collot
Iain B H
IB
Wilson
Merima
M
Bublin
Karin
K
Hoffmann-Sommergruber
Jean-Maurice
JM
Mallet
D000485:Allergens / Q000276:immunology; D000906:Antibodies / Q000276:immunology*; D001514:Bee Venoms / Q000276:immunology; D001710:Biotin / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005643:Fucose / Q000031:analogs & derivatives / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D006969:Hypersensitivity, Immediate / Q000175:diagnosis; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000276:immunology*; D019809:Streptavidin; D014312:Trisaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology*; D014994:Xylose / Q000031:analogs & derivatives / Q000276:immunology
D000485:Allergens; D000906:Antibodies; D001514:Bee Venoms; D000939:Epitopes; D011134:Polysaccharides; D014312:Trisaccharides; D005643:Fucose; D007073:Immunoglobulin E; D001710:Biotin; D019809:Streptavidin; D014994:Xylose; D006735:Horseradish Peroxidase