Identification and characterization of Ixodes scapularis antigens that elicit tick immunity using yeast surface display.
Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America. T.J.Schuijt@amc.uva.nl
Tim J
TJ
Schuijt
Sukanya
S
Narasimhan
Sirlei
S
Daffre
Kathleen
K
DePonte
Joppe W R
JW
Hovius
Cornelis
C
Van't Veer
Tom
T
van der Poll
Kamran
K
Bakhtiari
Joost C M
JC
Meijers
Eric T
ET
Boder
Alje P
AP
van Dam
Erol
E
Fikrig
D000818:Animals; D000941:Antigens / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D007109:Immunity; D007114:Immunization / Q000379:methods*; D018884:Ixodes / Q000276:immunology*; D009758:Nymph; D019151:Peptide Library; D011817:Rabbits; D012469:Salivary Glands / Q000276:immunology; D013987:Ticks / Q000276:immunology; D015003:Yeasts
D000941:Antigens; D019151:Peptide Library
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