[Study of immunogenicity of chlorogenic acid-BSA with different number of CGA].
OBJECTIVE
To investigate the immunogenicity of chlorogenic acid-BSA(CGA-BSA) and the foundation for the allergization of CGA-BSA.


METHODS
The CGA-BSA with different number of CGA was synthesized to allergize the BALB/c mice and SD rats to get antiserum. The level of IgE and histamine was analyzed by ELISA, and the passive cutaneous anaphylaxis (PCA) test was carried to analyze the antibody titer.


RESULTS
When the coupling rate of CGA-BSA is 20, the highest IgE and histamine level in BALB/c mice and the antibody titer in PCA are the highest.


CONCLUSIONS
It has better immunogenicity when the coupling rate of CGA-BSA is between 10 to 20, while the CGA-BSA 20 with the best immunogenicity.
Department of Clinical Pharmacology, General Hospital of PLA, Beijing 100853, China.
Liyan
L
Yuan
Ping
P
Liu
Yuan
Y
Hu
Hongbo
H
Liao
Lihua
L
Mu
Hui
H
Lin
Honglin
H
Huang
D000818:Animals; D002726:Chlorogenic Acid / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000276:immunology*
D012710:Serum Albumin, Bovine; D002726:Chlorogenic Acid; D007073:Immunoglobulin E; D006632:Histamine