Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
Sesbania mosaic virus (SeMV) is a single strand positive-sense RNA plant virus that belongs to the genus Sobemovirus. The mechanism of cell-to-cell movement in sobemoviruses has not been well studied. With a view to identify the viral encoded ancillary proteins of SeMV that may assist in cell-to-cell movement of the virus, all the proteins encoded by SeMV genome were cloned into yeast Matchmaker system 3 and interaction studies were performed. Two proteins namely, viral protein genome linked (VPg) and a 10-kDa protein (P10) c v gft encoded by OFR 2a, were identified as possible interacting partners in addition to the viral coat protein (CP). Further characterization of these interactions revealed that the movement protein (MP) recognizes cognate RNA through interaction with VPg, which is covalently linked to the 5' end of the RNA. Analysis of the deletion mutants delineated the domains of MP involved in the interaction with VPg and P10. This study implicates for the first time that VPg might play an important role in specific recognition of viral genome by MP in SeMV and shed light on the possible role of P10 in the viral movement.
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Soumya
S
Roy Chowdhury
Handanahal S
HS
Savithri
D036022:Capsid Proteins / Q000378:metabolism; D016679:Genome, Viral; D009029:Mosaic Viruses / Q000235:genetics / Q000378:metabolism*; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D011485:Protein Binding; D049931:Sesbania / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
D036022:Capsid Proteins; D053830:Plant Viral Movement Proteins; D017361:Viral Nonstructural Proteins
Virology. 2008 Aug 1;377(2):280-8
18533220
Nature. 1970 Aug 15;227(5259):680-5
5432063
Structure. 1995 Oct 15;3(10):1021-30
8589997
J Virol. 2000 Sep;74(17):7730-7
10933678
Virus Res. 2007 Jan;123(1):95-9
16971015
Plant Cell. 1996 Oct;8(10):1669-1681
12239357
J Virol. 2004 Mar;78(5):2301-9
14963126
FEBS Lett. 1988 Aug 1;235(1-2):16-24
2841153
Virus Res. 2000 Feb;66(2):197-207
10725552
J Gen Virol. 2003 Aug;84(Pt 8):2271-2277
12867661
FASEB J. 1993 Jul;7(10):957-63
8344494
Virology. 1994 Oct;204(1):254-65
7522371
Virology. 2008 Dec 5;382(1):83-90
18842279
J Proteome Res. 2007 May;6(5):1899-916
17391015
J Biol Chem. 2005 Aug 26;280(34):30291-300
15944159
J Gen Virol. 2003 Jun;84(Pt 6):1351-1366
12771402
Virology. 1998 Sep 1;248(2):312-22
9721240
Eur J Biochem. 2002 Jan;269(1):2-12
11784292
FEBS Lett. 2010 Feb 5;584(3):571-6
19995563
Virus Genes. 2006 Jun;32(3):321-6
16732485
J Gen Virol. 1992 Feb;73 ( Pt 2):223-7
1538188
EMBO J. 2001 Dec 17;20(24):6997-7007
11742977
Plant J. 1998 May;14(4):393-400
9670556
Trends Microbiol. 2001 Jan;9(1):5-8; discussion 8
11166222
Virology. 2010 Jan 5;396(1):106-17
19861224
Virology. 1997 Oct 27;237(2):283-95
9356340
J Mol Biol. 2005 Oct 21;353(2):447-58
16169007
Arch Virol. 2001;146(2):209-23
11315633
J Mol Biol. 2004 Sep 17;342(3):1001-14
15342252
J Biochem. 1988 Sep;104(3):451-6
3149277
Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14147-52
10570213
J Gen Virol. 2006 Feb;87(Pt 2):363-368
16432023
Trends Plant Sci. 2005 Aug;10(8):376-82
16023398
Virology. 2010 Jun 5;401(2):280-92
20332053
EMBO Rep. 2005 Oct;6(10):968-72
16142217
J Virol. 2001 Sep;75(18):8712-23
11507216
Biochemistry (Mosc). 2009 Oct;74(10):1132-41
19916926
J Biol Chem. 2008 Jan 4;283(1):213-221
17971447
Virology. 2006 Jan 5;344(1):169-84
16364748
Proteins. 2000 Nov 15;41(3):415-27
11025552
FEBS Lett. 1988 Nov 21;240(1-2):33-40
3191997
Virology. 1998 Dec 20;252(2):376-86
9878617
Trends Biochem Sci. 2002 Oct;27(10):527-33
12368089
Virology. 1995 Feb 1;206(2):1007-16
7856075
Arch Virol. 1986;90(3-4):301-12
3729730
Virology. 1998 Apr 25;244(1):79-86
9581781
EMBO J. 1994 Mar 15;13(6):1482-91
7511101
Nucleic Acids Res. 1989 Jun 26;17(12):4713-30
2546125
Cell. 1998 Oct 2;95(1):105-13
9778251
Anal Biochem. 1987 Apr;162(1):156-9
2440339