2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes.
Cytotoxic T cells play a critical role in the control of HIV and the progression of infected individuals to AIDS. 2B4 (CD244) is a member of the SLAM family of receptors that regulate lymphocyte development and function. The expression of 2B4 on CD8+ T cells was shown to increase during AIDS disease progression. However, the functional role of 2B4+ CD8+ T cells against HIV infection is not known. Here, we have examined the functional role of 2B4+ CD8+ T cells during and after stimulation with HLA B14 or B27 restricted HIV epitopes. Interestingly, IFN-γ secretion and cytotoxic activity of 2B4+ CD8+ T cells stimulated with HIV peptides were significantly decreased when compared to influenza peptide stimulated 2B4+ CD8+ T cells. The expression of the signaling adaptor molecule SAP was downregulated in 2B4+ CD8+ T cells upon HIV peptide stimulation. These results suggest that 2B4+ CD8+ T cells play an inhibitory role against constrained HIV epitopes underlying the inability to control the virus during disease progression.
Department of Surgery-Burn/Trauma/Critical care, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
Kim N
KN
Aldy
Nathan C
NC
Horton
Porunelloor A
PA
Mathew
Stephen O
SO
Mathew
D015703:Antigens, CD / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology; D015536:Down-Regulation; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology; D059909:HLA-B14 Antigen; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology*; D000071179:Signaling Lymphocytic Activation Molecule Associated Protein; D000071176:Signaling Lymphocytic Activation Molecule Family; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015703:Antigens, CD; C084093:CD244 protein, human; D016827:CD8 Antigens; D018984:Epitopes, T-Lymphocyte; D015235:HLA-B Antigens; D059909:HLA-B14 Antigen; D047908:Intracellular Signaling Peptides and Proteins; D011971:Receptors, Immunologic; C114953:SH2D1A protein, human; D000071179:Signaling Lymphocytic Activation Molecule Associated Protein; D000071176:Signaling Lymphocytic Activation Molecule Family
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