Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip.
We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.
Chaitanya
C
Dongre
Jasper
J
van Weerd
Nicola
N
Bellini
Roberto
R
Osellame
Giulio
G
Cerullo
Rob
R
van Weeghel
Hugo J W M
HJ
Hoekstra
Markus
M
Pollnau
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