Analysis of plasminogen activator inhibitor-1, integrin beta3, beta fibrinogen, and methylenetetrahydrofolate reductase polymorphisms in Iranian women with recurrent pregnancy loss.
OBJECTIVE
To identify the associations of the plasminogen activator inhibitor-1 (PAI-1) -675 4G/5G, beta fibrinogen (BF) -455G/A, integrin beta 3 (ITGB3) 1565T/C, and methylenetetrahydrofolate reductase (MTHFR) 677C/T and 1298A/C polymorphisms with recurrent pregnancy loss (RPL).


METHODS
Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed to assess the frequency of five candidate genetic risk factors for RPL, and the frequencies of the polymorphisms were calculated and compared between case and control groups.


RESULTS
The BF -455G/A, MTHFR 677C/T, and 1298A/C polymorphisms were found to be positively, and ITGB3 1565T/C polymorphism negatively, associated with RPL. Homozygosity but not heterozygosity for PAI-1 -675 4G/5G polymorphism was significantly higher in patients with RPL than in the control group. The presence of both mutations of MTHFR genes highly increased the risk of RPL.


CONCLUSIONS
The data highlight the importance of thrombophilia screening in patients with RPL.
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Shahid Beheshti University, Evin, Tehran, Iran. mahjed@yahoo.com
Mahmood
M
Jeddi-Tehrani
Raheleh
R
Torabi
Amir H
AH
Zarnani
Afsaneh
A
Mohammadzadeh
Soheila
S
Arefi
Hojjat
H
Zeraati
Mohammad M
MM
Akhondi
Leili
L
Chamani-Tabriz
Farah
F
Idali
Shaghayegh
S
Emami
Saeed
S
Zarei
D000026:Abortion, Habitual / Q000235:genetics*; D005260:Female; D005340:Fibrinogen / Q000235:genetics*; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011247:Pregnancy; D012307:Risk Factors
D039661:Integrin beta3; D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human; D005340:Fibrinogen; D042965:Methylenetetrahydrofolate Reductase (NADPH2)