Real-time QCM-D monitoring of cellular responses to different cytomorphic agents.
Quartz crystal microbalance with dissipation monitoring (QCM-D) is used for real-time in situ detection of cytoskeletal changes in live primary endothelial cells in response to different cytomorphic agents; namely, the surfactant Triton-X 100 (TX-100) and bacterial lipopolysaccharide (LPS). Reproducible dissipation versus frequency (Df) plots provide unique signatures of the interactions between endothelial cells and cytomorphic agents. While the QCM-D response for TX-100 can be described in two steps (changes in the osmotic pressure of the medium prior to observing the expected cell lysis), LPS results in a different single-phase signal. A complementary analysis is carried out to evaluate the possible competitive effects of TX-100 and LPS through the QCM-D response to BAEC stress by analyzing the Df plots obtained. Experiments with non-toxic components (fibronectin or serum) produce a different QCM-D response than that observed for the toxic chemicals, suggesting the use of Df plot signatures for the possible differentiation between cytotoxic or non-cytotoxic effects. Observations obtained by QCM-D signals are confirmed by conducting fluorescence microscopy at the same time. Our results show that a fast (few minutes) sensing response can be obtained in situ and in real-time. The conclusions from this study suggest that QCM-D can potentially be used in biodetection for applications in drug screening tests and diagnosis.
Department of Chemical Engineering, McGill University, Montreal, Quebec, Canada. julien.fatisson@gmail.com
Julien
J
Fatisson
Fereshteh
F
Azari
Nathalie
N
Tufenkji
D000818:Animals; D001011:Aorta / Q000166:cytology; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D017830:Octoxynol / Q000494:pharmacology*; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D013501:Surface-Active Agents / Q000494:pharmacology*
D008070:Lipopolysaccharides; D013501:Surface-Active Agents; D017830:Octoxynol