Subcellular proteomics revealed the epithelial-mesenchymal transition phenotype in lung cancer.
Subcellular proteomics was used to compare the protein profiles between human lung adenocarcinoma A549 cells and human bronchial epithelial (HBE) cells. In total, 106 differential proteins were identified and the altered expression levels of partial identified proteins were confirmed by Western blot analysis. Importantly, pathway analysis and biological validation revealed epithelial-mesenchymal transition (EMT) phenotype shift in A549 cells as compared with HBE cells. The EMT phenotype of A549 cells can be increased by self-producing TGF-β1 and significantly decreased by silencing heterogeneous nuclear ribonucleoprotein (hnRNPK) expression. As EMT has been considered as an important event during malignant tumor progression and metastasis, investigating EMT and deciphering the related pathways may lead to more efficient strategies to fight lung cancer progression. By integrating the subcellular proteomic data with EMT-related functional studies, we revealed new insights into the EMT progress of lung carcinogenesis, providing clues for further investigations on the discovery of potential therapeutic targets.
Institute of Life and Health Engineering/National Engineering and Research Center for Genetic Medicine, Jinan University, Guangzhou, PR China.
Li-Ping
LP
Li
Chun-Hua
CH
Lu
Zhi-Peng
ZP
Chen
Feng
F
Ge
Tong
T
Wang
Wei
W
Wang
Chuan-Le
CL
Xiao
Xin-Feng
XF
Yin
Langxia
L
Liu
Jian-Xing
JX
He
Qing-Yu
QY
He
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D001980:Bronchi / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013347:Subcellular Fractions
D020543:Proteome