Vascular-specific activity of the Arabidopsis carotenoid cleavage dioxygenase 7 gene promoter.
Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of this family in Arabidopsis by RT-PCR. We found that the AtCcd7 gene showed strong expression in seeds. However, the promoter activity of the 1,867-bp 5'-upstream region of this gene exhibited a vascular specificity at all developmental stages throughout the transgenic Arabidopsis plants tested. The strength of the AtCcd7 promoter was also found to be lower than that of the 35S promoter by about 60%. The whole body expression of the β-glucuronidase (GUS) reporter gene driven by the AtCcd7 promoter in Arabidopsis plants was confirmed in different organs by RT-PCR and GUS enzymatic assays. Histochemical GUS staining further revealed that the AtCcd7 promoter has utility in limiting the expression of target genes to the vascular tissues in all plant organs such as the leaf, stem, root, flower and seed.
National Academy of Agricultural Science, Rural Development Administration, 225 Seodun-dong, Suwon 441-707, South Korea.
Ying Shi
YS
Liang
Yun-A
YA
Jeon
Sun-Hyung
SH
Lim
Jae Kwang
JK
Kim
Jong-Yeol
JY
Lee
Young-Mi
YM
Kim
Yeon-Hee
YH
Lee
Sun-Hwa
SH
Ha
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins; D049308:Dioxygenases; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D005966:Glucuronidase / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010105:Oxygenases / Q000235:genetics*; D058526:Plant Vascular Bundle / Q000201:enzymology* / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins; D010105:Oxygenases; C494441:CCD7 protein, Arabidopsis; D049308:Dioxygenases; D005966:Glucuronidase
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