Effect of immobilized granulocyte colony-stimulating factor on hemopoietic precursors of various classes during cytostatic-induced myelosuppression.
Experiments were performed on the model of cytostatic myelosuppression induced by cyclophosphamide. We compared the effect of immobilized granulocyte CSF (the preparation was created in Russia) and reference standard preparation of granulocyte CSF on the development of neutrophilic leukopenia and hemopoietic precursors of various classes. It was found that preparations of granulocyte CSF decreased the duration and degree of peripheral blood neutropenia. The granulocytopoiesis-stimulating effect was related to stimulation of multipotent hemopoietic precursors, granulocyte-erythroid-macrophage-megakaryocyte precursors, and granulocyte precursors. Induction of division and maturation of multipotent hemopoietic precursors, granulocyte-erythroid-macrophage-megakaryocyte precursors, and granulocyte precursors and recovery of cellularity of the granulocytic hemopoietic stem after administration of immobilized granulocyte CSF were observed at later terms compared to treatment with the reference preparation of granulocyte CSF.
Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia. mmu@pharm.tsu.ru
A M
AM
Dygai
E G
EG
Skurikhin
T V
TV
Andreeva
P G
PG
Madonov
E I
EI
Vereshagin
D N
DN
Kinsht
O V
OV
Pershina
E S
ES
Khmelevskaya
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003520:Cyclophosphamide / Q000633:toxicity; D054697:Cytostatic Agents / Q000633:toxicity; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000627:therapeutic use; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009503:Neutropenia / Q000188:drug therapy; D018709:Statistics, Nonparametric
D054697:Cytostatic Agents; D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide