A potato skin SSH library yields new candidate genes for suberin biosynthesis and periderm formation.
Potato (Solanum tuberosum) tubers are underground storage organs covered by the skin or periderm, a suberized layer that protects inner flesh from dehydration and pathogens. Understanding the molecular processes associated with periderm formation is of great importance for a better knowledge of this protective tissue and for improving the storage life of tubers. Here, to isolate new candidate genes for potato periderm, a suppression subtractive hybridization library from potato skin was performed. This library yielded a comprehensive list of 108 candidate genes that were manually sorted in functional categories according to the main cellular and metabolic processes in periderm. As expected, the list contains Suberin and wax genes, including some genes with a demonstrated role in the biosynthesis of these cell wall aliphatic compounds. Moreover, Regulation and Stress and defence genes are highly abundant in the library in general agreement with previous potato skin proteomic studies. The putative function of the genes in periderm is discussed.
Laboratori del Suro, Department of Biology, Facultat de Ciències, Universitat de Girona, Campus Montilivi sn, 17071 Girona, Spain.
Marçal
M
Soler
Olga
O
Serra
Silvia
S
Fluch
Marisa
M
Molinas
Mercè
M
Figueras
D001483:Base Sequence; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008055:Lipids / Q000096:biosynthesis / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D035281:Plant Tubers / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D014885:Waxes / Q000378:metabolism
D018076:DNA, Complementary; D008055:Lipids; D010940:Plant Proteins; D014885:Waxes; C065875:suberin
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