Purification of a lectin from Canavalia ensiformis using PEG-citrate aqueous two-phase system.
A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.
Departamento de Bioquímica, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
Paulo A G
PA
Soares
Cynthia O
CO
Nascimento
Tatiana S
TS
Porto
Maria T S
MT
Correia
Ana L F
AL
Porto
Maria G
MG
Carneiro-da-Cunha
D000704:Analysis of Variance; D032449:Canavalia / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D019343:Citric Acid / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
D010936:Plant Extracts; D003208:Concanavalin A; D019343:Citric Acid; D011092:Polyethylene Glycols