Determination of the epimerization rate constant of amygdalin by microemulsion electrokinetic chromatography.
A new method for separation and determination of amygdalin and its epimer (neoamygdalin) in the epimerization of amygdalin by MEEKC is proposed. For the chiral separation of amygdalin and neoamygdalin, a running buffer composed of 80 mM sodium cholate, 5.0% v/v butan-1-ol, 0.5% v/v heptane and 94.5% v/v 30 mM Na(2) B(4) O(7) buffer (pH 9.00) is proposed. Under optimum conditions, the basic separation of amygdalin and neoamygdalin can be achieved within 7 min. The calibration curve for amygdalin showed excellent linearity in the concentration range of 20-1000 μg/mL with a detection limit of 5.0 μg/mL (S/N=3). The epimerization rate constant of amygdalin in basic microemulsion was first determined by monitoring the concentration changes of amygdalin, and the epimerization rate constant of amygdalin was found to be 2×10(-3) min(-1) at 25°C under the above optimum microemulsion conditions.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, P. R. China.
Lishuang
L
Yu
Hongzhi
H
Ye
Lili
L
Zheng
Lidian
L
Chen
Kedan
K
Chu
Xianxiang
X
Liu
Xueqin
X
Xu
Guonan
G
Chen
D000678:Amygdalin / Q000032:analysis / Q000737:chemistry*; D002021:Buffers; D053801:Capillary Electrochromatography / Q000379:methods*; D004655:Emulsions / Q000737:chemistry; D007700:Kinetics
D002021:Buffers; D004655:Emulsions; D000678:Amygdalin