Randomly-amplified microsatellite polymorphism for preliminary typing of lactic acid bacteria from Bryndza Cheese.
A high-throughput, medium-discrimination method for preliminary typing and selecting non-identical isolates of lactic acid bacteria in cheeses was developed. RAMP, a PCR with one microsatellite-targeted and one random primer, was used for preliminary typing of 1119 isolates of lactic acid bacteria from Slovak Bryndza cheese. A total of 59 genotypes were identified based on RAMP profiles consisting of 12-23 DNA fragments of 150-3000 bp. For example, 18, 17, 13 and 7 different RAMP-types were identified in Lactobacillus brevis, L. plantarum, L. paracasei and L. fermentum, respectively. The method facilitated well reproducible, medium-discrimination typing of Lactobacillus spp. and Pediococcus spp. at a subspecies level and proved to be suitable for preliminary typing of lactic acid bacteria isolated from cheese.
Department of Microbiology and Molecular Biology, Food Research Institute, Bratislava, Slovakia.
V
V
Chebeňová
G
G
Berta
T
T
Kuchta
B
B
Brežná
D
D
Pangallo
D002611:Cheese / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005838:Genotype; D019344:Lactic Acid / Q000378:metabolism; D007777:Lactobacillaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D018895:Microsatellite Repeats; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D017931:DNA Primers; D004269:DNA, Bacterial; D019344:Lactic Acid
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