[Clinical significance and relationship between HIF-1alpha, COX-2, VEGF and microvessel density expression in laryngeal carcinoma].
OBJECTIVE
To evaluate the role of HIF-1alpha, COX-2, VEGF proteins and Microvessel density in angiogenesis and metastasis of laryngeal carcinoma.


METHODS
Surgically resected specimens of 64 laryngeal carcinomas were studied by immunohistochemical staining for HIF-1alpha protein, COX-2 protein, VEGF and MVD.


RESULTS
Positive expressions of HIF-1alpha, COX-2 protein and VEGF were found in 65.63%, 68.75% and 71.88% of laryngeal carcinoma specimens, respectively. The mean MVD in this series was 35.79 +/- 9.49. The expression of HIF-1alpha, COX-2 and VEGF positively correlated with MVD expression. There was a positive relationship between HIF-1alpha, COX-2 and VEGF expression. The microvessel density (MVD) in HIF-1alpha, COX-2 and VEGF positive tumors was significantly higher in patients with cervical lymph node metastases than that in patients without metastases.


CONCLUSIONS
HIF-1alpha, COX-2 and VEGF proteins may play an important role in the angiogenesis of laryngeal carcinoma. HIF-1alpha regulate the expression and function of its downstream target genes (COX-2 and VEGF), which play a critical role in the angiogenesis of laryngeal carcinoma, metastases in the process of laryngeal cancer cell spreading to cervical lymph nodes.
Department of Otolaryngology, Nanhai Hospital of Southern Medical University, Foshan, 528200, China. nhyycw518@126.com
Wei
W
Cheng
Weiguo
W
Zhou
D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D008297:Male; D055806:Microvessels; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human