Quantum dot-based array for sensitive detection of Escherichia coli.
A fluorescent quantum dot-based antibody array, used in sandwich format, has been developed to detect Escherichia coli O157:H7. Numerous parameters such as solid support, optimal concentration of immunoreagents, blocking reagents, and assay time were optimized for array construction. Quantum dot-conjugated anti-IgG was used as the detecting system. The array allows the detection of E. coli O157:H7 at concentrations below 10 CFU mL(-1) without sample enrichment, exhibiting an increase of three orders of magnitude in the limit of detection compared to ELISA. The interference caused by Gram (+) and Gram (-) bacteria was negligible at low concentrations of bacteria.
Applied Molecular Receptors Group, Institute for Advanced Chemistry of Catalonia of the Spanish Council for Scientific Research, Barcelona, Spain.
Nuria
N
Sanvicens
Núria
N
Pascual
María Teresa
MT
Fernández-Argüelles
Javier
J
Adrián
José Manuel
JM
Costa-Fernández
Francisco
F
Sánchez-Baeza
Alfredo
A
Sanz-Medel
M-Pilar
MP
Marco
D019453:Escherichia coli O157 / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification*; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D045663:Quantum Dots; D012680:Sensitivity and Specificity