Different expressions and DNA methylation patterns of lysophosphatidic acid receptor genes in mouse tumor cells.
OBJECTIVE
Lysophosphatidic acid (LPA) receptors act as several biological effectors through LPA, which is a bioactive phospholipid. Recently, aberrant expressions of LPA receptor genes due to DNA methylation have been detected in several tumor cells. In this study, we measured expression levels and DNA methylation status of LPA receptor genes in mouse tumor cells, LL/2 lung carcinoma, B16F0 melanoma, FM3A mammary carcinoma and L1210 leukemia cells, compared with normal tissues.


METHODS
Total RNAs were extracted and RT-PCR analysis was performed. For DNA methylation status, bisulfite sequencing analysis was carried out, comparing outcomes with other tumor cells and normal tissues.


RESULTS
The expressions of LPA1 gene were shown in LL/2, but not in B16F0, FM3A and L1210 cells. While the LPA2 gene was expressed in all 4 tumor cells, the LPA3 gene was unexpressed in them. The LPA1 and LPA3 unexpressed cells were highly methylated, although normal tissues were all unmethylated. The DNA methylation status was correlated with gene expression levels in cancer cells.


CONCLUSIONS
The present results demonstrate that DNA methylation patterns of LPA receptor genes are dependent on cancer cell types, suggesting that LPA receptors may be new molecular targets for therapeutic approaches and chemoprevention.
Divisions of Cancer Biology and Bioinformatics, Faculty of Science and Technology, Kinki University, Higashiosaka, Japan.
Kyoko
K
Okabe
Mai
M
Hayashi
Naoko
N
Wakabayashi
Yasuna
Y
Yamawaki
Miki
M
Teranishi
Nobuyuki
N
Fukushima
Toshifumi
T
Tsujiuchi
D000818:Animals; D001483:Base Sequence; D018827:Carcinoma, Lewis Lung / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D007939:Leukemia L1210 / Q000235:genetics / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism*; D049368:Receptors, Lysophosphatidic Acid / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D004273:DNA, Neoplasm; D049368:Receptors, Lysophosphatidic Acid