Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy.
We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.
Keisuke
K
Isobe
Hiroshi
H
Hashimoto
Akira
A
Suda
Fumihiko
F
Kannari
Hiroyuki
H
Kawano
Hideaki
H
Mizuno
Atsushi
A
Miyawaki
Katsumi
K
Midorikawa
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