Chemically modulating the photophysics of the GFP chromophore.
There is growing interest in engineering the properties of fluorescent proteins through modifications to the chromophore structure utilizing mutagenesis with either natural or unnatural amino acids. This entails an understanding of the photophysical and photochemical properties of the modified chromophore. In this work, a range of GFP chromophores with different alkyl substituents are synthesized and their electronic spectra, pH dependence, and ultrafast fluorescence decay kinetics are investigated. The weakly electron donating character of the alkyl substituents leads to dramatic red shifts in the electronic spectra of the anions, which are accompanied by increased fluorescence decay times. This high sensitivity of electronic structure to substitution is also characteristic of some fluorescent proteins. The solvent viscosity dependence of the decay kinetics are investigated, and found to be consistent with a bimodal radiationless relaxation coordinate. Some substituents are shown to distort the planar structure of the chromophore, which results in a blue shift in the electronic spectra and a strong enhancement of the radiationless decay. The significance of these data for the rational design of novel fluorescent proteins is discussed.
School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
Jamie
J
Conyard
Minako
M
Kondo
Ismael A
IA
Heisler
Garth
G
Jones
Anthony
A
Baldridge
Laren M
LM
Tolbert
Kyril M
KM
Solntsev
Stephen R
SR
Meech
D019943:Amino Acid Substitution; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009154:Mutation; D014783:Viscosity
D049452:Green Fluorescent Proteins