Dihydroavenanthramide D inhibits human breast cancer cell invasion through suppression of MMP-9 expression.
Dihydroavenanthramide D (DHAvD) is a synthetic analog to naturally occurring avenanthramide, which is the active component of oat. Previous study demonstrates that DHAvD strongly inhibits activation of nuclear factor-kappa B (NF-κB), which is a major component in cancer cell invasion. The present study investigated whether DHAvD can modulate MMP-9 expression and cell invasion in MCF-7 human breast cancer cells. MMP-9 expression and cell invasion in response to 12-O-tetradecanoylphorbol-13-acetate (TPA) was increased, whereas these inductions were muted by DHAvD. DHAvD also suppressed activation of mitogen-activated protein kinase (MAPK), and MAPK-mediated nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) activations in TPA-treated MCF-7 cells. The results indicate that DHAvD-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the MAPK/NF-κB and MAPK/AP-1 pathways in MCF-7 cells. DHAvD may have potential value in breast cancer metastasis.
Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University, Medical School, Jeonju, Jeonbuk 560-182, Republic of Korea.
Young-Rae
YR
Lee
Eun-Mi
EM
Noh
Hyun Ju
HJ
Oh
Hyun
H
Hur
Jeong-Mi
JM
Kim
Ji-Hey
JH
Han
Jin-Ki
JK
Hwang
Byung-Hyun
BH
Park
Jin-Woo
JW
Park
Hyun Jo
HJ
Youn
Sung Hoo
SH
Jung
Byeong-Soo
BS
Kim
Ji-Youn
JY
Jung
Sung-Ho
SH
Lee
Chang-Sik
CS
Park
Jong-Suk
JS
Kim
D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D061965:Matrix Metalloproteinase Inhibitors; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D061965:Matrix Metalloproteinase Inhibitors; D062367:ortho-Aminobenzoates; C532345:dihydroavenanthramide D; D020928:Mitogen-Activated Protein Kinases; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate