Tools for studying the role of N-cadherin mediated extracellular interaction in neuronal development and function.
N-cadherin is a homophilic cell adhesion molecule that plays important roles in many aspects of neuronal development. In order to better understand the function of N-cadherin mediated cell-cell contact in activity-dependent dendrite development, we generated a number of new tools. EC1, consisting of the first extracellular domain of N-cadherin, can specifically inhibit N-cadherin, but not E-cadherin, mediated cell-cell contact, both when over-expressed in neurons and added as a purified protein. Ncad-HA is an extracellularly epitope-tagged version of N-cadherin that, when over-expressed under the activity-independent pCS2min promoter, can be used to assay surface N-cadherin level following various manipulations. These tools are likely to be very useful for studying the function of N-cadherin in multiple aspects of neural circuit development.
Institute of Neuroscience and State Key Laboratory of Neuroscience, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. yuxiang@ion.ac.cn
Xiang
X
Yu
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D006801:Humans; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*
D015820:Cadherins
Nature. 1995 Mar 23;374(6520):327-37
7885471
Trends Cell Biol. 2005 Feb;15(2):64-7
15695092
J Cell Biol. 1988 Mar;106(3):873-81
2831236
Nature. 1986 Apr 3-9;320(6061):447-9
3515198
Curr Opin Neurobiol. 2005 Feb;15(1):73-80
15721747
J Neurocytol. 2000 Mar;29(3):215-23
11428051
J Cell Biol. 1990 Apr;110(4):1253-60
2324197
Neuron. 1998 Jun;20(6):1165-75
9655504
Hippocampus. 2008;18(4):349-63
18064706
J Biol Chem. 2000 Feb 11;275(6):4007-12
10660557
Proc Natl Acad Sci U S A. 2010 May 25;107(21):9873-8
20457910
Proc Natl Acad Sci U S A. 1985 May;82(9):2789-93
3857614
Neuron. 1988 Mar;1(1):33-43
2856086
Mol Cell Neurosci. 1999 Jul;14(1):66-84
10433818
Dev Biol. 1990 May;139(1):227-9
2328837
Neuron. 1991 Feb;6(2):247-58
1993122
Neuron. 1998 Jun;20(6):1153-63
9655503
Cell. 1990 Apr 6;61(1):147-55
2317870
Cold Spring Harb Perspect Biol. 2009 Sep;1(3):a003053
20066110
J Biol Chem. 2001 Aug 24;276(34):31978-85
11397804
Trends Neurosci. 2008 Sep;31(9):487-94
18684518
J Neurosci. 1998 Sep 1;18(17):6892-904
9712659