An improved method of ion exchange for nitrogen isotope analysis of water nitrate.
Nitrate nitrogen and oxygen isotopes have been widely used to trace the nitrogen biogeochemical cycle by identifying NO(3)(-) sources. An improved method of anion exchange was developed to measure δ(15)N-NO(3)(-) in fresh water by continuous-flow elemental analyzer/isotope ratio mass spectrometry (EA-IRMS). We used a custom-built exchange resin column, a peristaltic pump and the oven-drying method in our experiments. Consequently, the amount of Ag(2)O used as a neutralizer was reduced, time was saved, and operation became simpler than before. Meanwhile, analytical precision remained identical to previous studies. KNO(3) solutions were prepared at 0.2, 5 and 25 mg-N L(-1) from KNO(3) standard salt (δ(15)N=+6.27‰), and the average δ(15)N values of the solutions after having been absorbed on and subsequently stripped from anion columns were +6.62±0.22‰ (n=6), +6.38±0.09‰ (n=6), and +6.26±0.07‰ (n=6), respectively. In addition, the "natural" water sample δ(15)N-NO(3)(-) showed consistency in comparison to standards, and the mean standard deviation by the different approaches was 0.08‰. Accordingly, by these improvements the anion exchange resin technique is demonstrated to be more suitable for measuring δ(15)N in NO(3)(-) than original techniques.
State Key Laboratory of Loess and Quaternary Geology, IEE, CAS, Xi'an, 710075, China.
Meng
M
Xing
Weiguo
W
Liu
D002852:Chromatography, Ion Exchange / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009566:Nitrates / Q000737:chemistry*; D009584:Nitrogen / Q000032:analysis*; D009587:Nitrogen Isotopes / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D009566:Nitrates; D009587:Nitrogen Isotopes; D010087:Oxides; D018030:Silver Compounds; D014867:Water; C040225:disilver oxide; D009584:Nitrogen