The comparison of the negative effect of autoclaving and pasteurization on bone healing.
OBJECTIVE
The aim of this study was to compare the effects of autoclaving and pasteurization on bone healing.


METHODS
Twenty-five full-grown male rabbits were included in the study; all 25 had bone blocks resected and reimplanted. In group 1, bone blocks were autoclaved; in group 2, bone blocks were pasteurized; and in group 3 (controls), resected bone blocks were reimplanted without sterilization.


RESULTS
Heiple scores of the proximal parts of the fusion surfaces in group 1, group 2, and group 3 were 12.8±0.4, 6.8±1.2, and 10.2±1.9, respectively. Heiple scores of the distal parts of the fusion surfaces in group 1, group 2, and group 3 were 10.8±0.8, 6.0±1.1, and 9.8±1.5, respectively. Differences in radiologic scores were not statistically significant between the groups for proximal or distal fusion surfaces at 3 and 6 weeks.


CONCLUSIONS
In conclusion, pasteurization has a less negative effect on bone healing than autoclaving, and can be considered for bone sterilization in certain circumstances.
Faculty of Medicine, Departments of Orthopedics and Traumatology, Uludağ University, Bursa, Turkey.
Recep
R
Vural
Burak
B
Akesen
Mehmet
M
Karakayalı
Ulviye
U
Yalçınkaya
Ufuk
U
Aydınlı
D000818:Animals; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000009:adverse effects*; D006358:Hot Temperature / Q000627:therapeutic use*; D008297:Male; D023421:Models, Animal; D010027:Osteotomy / Q000009:adverse effects*; D011817:Rabbits; D011859:Radiography; D013242:Sterilization / Q000379:methods / Q000592:standards; D014184:Transplantation, Homologous / Q000473:pathology*; D014457:Ulna / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery; D014945:Wound Healing