Phenotypic and molecular characterization of recent and archived Erysipelothrix spp. isolated from Brazilian swine.
One hundred fifty-one Erysipelothrix spp. isolates from Brazilian swine were characterized by serotyping, determination of antimicrobial susceptibility, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE). Among all isolates, 139 were classified in 18 different serotypes and serotype 2b was the most frequent. The susceptibility profiles of the isolates were very similar among each other, which did not permit subtyping Erysipelothrix spp. isolates by the antimicrobial susceptibility testing. Despite the fact that AFLP and PFGE provided the same discriminatory index (0.98), PFGE was more discriminatory than AFLP, given the types of groups it generates. Regardless the technique employed (AFLP or PFGE), no discrimination between recent and historical isolates was established, neither a fixed epidemiologic pattern for their grouping was observed. Nevertheless, AFLP could be an interesting alternative for discriminating the Erysipelothrix species, while PFGE could be an indication for discerning this bacterium according to the serotypes.
Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, CEP 05508 270, São Paulo/SP, Brazil.
Tania A
TA
Coutinho
Yumiko
Y
Imada
David E S N
DE
Barcellos
Sergio J
SJ
Oliveira
Andrea M
AM
Moreno
D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004888:Erysipelothrix / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D004889:Erysipelothrix Infections / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D010802:Phylogeny; D012703:Serotyping; D013552:Swine
D000890:Anti-Infective Agents; D004269:DNA, Bacterial