Olivibacter oleidegradans sp. nov., a hydrocarbon-degrading bacterium isolated from a biofilter clean-up facility on a hydrocarbon-contaminated site.
A novel hydrocarbon-degrading, Gram-negative, obligately aerobic, non-motile, non-sporulating, rod-shaped bacterium, designated strain TBF2/20.2(T), was isolated from a biofilter clean-up facility set up on a hydrocarbon-contaminated site in Hungary. It was characterized by using a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate is affiliated with the genus Olivibacter in the family Sphingobacteriaceae. It was found to be related most closely to Olivibacter ginsengisoli Gsoil 060(T) (93.3% 16S rRNA gene sequence similarity). Strain TBF2/20.2(T) grew at pH 6-9 (optimally at pH 6.5-7.0) and at 15-42 °C (optimally at 30-37 °C). The major fatty acids were iso-C(15:0) (39.4%), summed feature 3 (iso-C(15:0) 2-OH and/or C(16:1)ω7c; 26.0%), iso-C(17:0) 3-OH (14.5%) and C(16:0) (4.5%). The major menaquinone was MK-7 and the predominant polar lipid was phosphatidylethanolamine. The DNA G+C content of strain TBF2/20.2(T) was 41.2 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain TBF2/20.2(T) from recognized members of the genus Olivibacter. Thus, strain TBF2/20.2(T) is considered to represent a novel species of the genus Olivibacter, for which the name Olivibacter oleidegradans sp. nov. is proposed. The type strain is TBF2/20.2(T) (=NCAIM B 02393(T) =CCM 7765(T)).
Department of Environmental Protection and Environmental Safety, Szent István University, Páter K. u. 1., 2103 Gödöllő, Hungary.
István
I
Szabó
Department of Environmental Protection and Environmental Safety, Szent István University, Páter K. u. 1., 2103 Gödöllő, Hungary.
Sándor
S
Szoboszlay
Department of Environmental Protection and Environmental Safety, Szent István University, Páter K. u. 1., 2103 Gödöllő, Hungary.
Balázs
B
Kriszt
Regional University Center of Excellence, Szent István University, Gödöllő, Hungary.
Judit
J
Háhn
Regional University Center of Excellence, Szent István University, Gödöllő, Hungary.
Péter
P
Harkai
Regional University Center of Excellence, Szent István University, Gödöllő, Hungary.
Erzsébet
E
Baka
Regional University Center of Excellence, Szent István University, Gödöllő, Hungary.
András
A
Táncsics
Department of Environmental Protection and Environmental Safety, Szent István University, Páter K. u. 1., 2103 Gödöllő, Hungary.
Edit
E
Kaszab
Agruniver Holding Ltd, Ganz Á. u. 2., Gödöllő, Hungary.
Zoltán
Z
Privler
Agruniver Holding Ltd, Ganz Á. u. 2., Gödöllő, Hungary.
József
J
Kukolya
D001482:Base Composition; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D052918:Environmental Restoration and Remediation / Q000295:instrumentation*; D004864:Equipment and Supplies / Q000382:microbiology*; D005227:Fatty Acids / Q000378:metabolism; D006814:Hungary; D006838:Hydrocarbons / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S