[Simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos by HPLC].
OBJECTIVE
To develop an HPLC method for simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos.


METHODS
The analysis was carried out on a Phenomenex Synergi POLAR-RP 80A column (4.6 mm x 250 mm, 4 microm) with gradient elution using methanol-acetonitrile-water (adjusted to pH 2.5 with formic acid) as mobile phase. The flow rate was 1.2 mL x min(-1) and the detection wavelength was at 255 nm.


RESULTS
The calibration curves were linear over the range of 0.2-2 000 microg x L(-1) for rutin and isoquercitrin, 10-2 000 microg x L(-1) for chlorogenic acid, respectively. The average recoveries were 99.5% for rutin, 100.1% for isoquercitrin and 99.4% for chlorogenic acid, respectively, with RSD not more than 3.0%.


CONCLUSIONS
The described method is reliable and could be used for the quality control of Farfarae Flos.
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 211198, China. michael2141@hotmail.com
Di
D
Wu
Zhengtao
Z
Wang
Mian
M
Zhang
D002138:Calibration; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis; D012431:Rutin / Q000032:analysis*; D031207:Tussilago / Q000737:chemistry*
C016527:isoquercitrin; D002726:Chlorogenic Acid; D012431:Rutin; D011794:Quercetin