CCND1 amplification and protein overexpression in oral squamous cell carcinoma of young patients.
BACKGROUND
The purpose of this study was to evaluate the correlation of CCND1 amplification and protein overexpression with clinicopathological features and clinical outcomes in patients younger than 41 years old with oral squamous cell carcinoma (SCC).


METHODS
Eighty-six young patients with oral SCC were evaluated using the tissue microarray technique, immunohistochemistry, and fluorescence in situ hybridization (FISH). These cases were compared with 116 patients with oral cancer aged over 50 years old (controls).


RESULTS
Cyclin D1 overexpression was observed in 47.7% of tumors in the young group and in 32.8% of controls (p = .03). In the young group, CCND1 amplification and overexpression were higher than in the control patients and the differences were statistically significant. In the young group, protein overexpression correlated with diminished disease-free survival (DFS), whereas in the control patients, cyclin D1 overexpression correlated with diminished DFS and overall survival (OS).


CONCLUSIONS
In both groups, amplification had no influence on prognosis. Protein overexpression was an indicator of worse DFS in both groups.
Department of Head and Neck Surgery and Otorhinolaryngology, A.C. Camargo Hospital, São Paulo, SP, Brazil.
Estela
E
Kaminagakura
Isabela
I
Werneck da Cunha
Fernando Augusto
FA
Soares
Inês Nobuko
IN
Nishimoto
Luiz Paulo
LP
Kowalski
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000401:mortality; D016022:Case-Control Studies; D019938:Cyclin D1 / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000401:mortality; D020411:Oligonucleotide Array Sequence Analysis; D012189:Retrospective Studies; D055815:Young Adult
C530104:CCND1 protein, human; D019938:Cyclin D1