Characterization of the porcine p65 subunit of NF-κB and its association with virus antibody levels.
NF-κB p65 subunit plays important roles in controlling both innate and adaptive immunity. Here we report the characterization of porcine NF-κB p65 subunit (pp65). pp65 shows high similarity to other mammalian counterparts. pp65 mRNA expression was mainly observed in lung, spleen, liver and small intestine. Furthermore, overexpression of pp65 activates NF-κB in porcine endothelial cell line PIEC, porcine alveolar macrophages cell line 3D4/21 and porcine primary fetal fibroblasts. A COOH-terminal truncation derivative of pp65 (pp65RHD) has been identified as a specific transdominant inhibitor of NFκB. Association study was performed on the selected SNP and indel. The results revealed that the SNP BglI was significantly associated (P<0.05) with pig reproduction and respiratory syndrome virus antibody level (PRRSV-AB) (0 day and 17 days), the classical swine fever virus (CSFV) antibody blocking rates (CSFV-AB) (0 day and 17 days) and pseudorabies virus antibody level (PRV-AB) (0 day and 32 days).
Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Huazhong Agricultural University, 1 Shi-zi-shan Street, Wuhan 430070, Hubei, PR China.
Hegang
H
Li
Guojian
G
Ma
Duan
D
Gui
Shuanping
S
Zhao
Pan
P
Wang
Kongwang
K
He
Xuemin
X
Wang
Jinxue
J
Ruan
Jiyue
J
Cao
Shulin
S
Yang
Kui
K
Li
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D003001:Cloning, Molecular; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D016678:Genome / Q000235:genetics; D007150:Immunohistochemistry; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D051996:Transcription Factor RelA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D000914:Antibodies, Viral; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D008156:Luciferases