PANP is a novel O-glycosylated PILRα ligand expressed in neural tissues.
PILRα is an immune inhibitory receptor possessing an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain enabling it to deliver inhibitory signals. Binding of PILRα to its ligand CD99 is involved in immune regulation; however, whether there are other PILRα ligands in addition to CD99 is not known. Here, we report that a novel molecule, PILR-associating neural protein (PANP), acts as an additional ligand for PILRα. Transcription of PANP was mainly observed in neural tissues. PILRα-Ig fusion protein bound cells transfected with PANP and the transfectants stimulated PILRα reporter cells. Specific O-glycan structures on PANP were found to be required for PILR recognition of this ligand. These results suggest that PANP is involved in immune regulation as a ligand of the PILRα.
Department of Immunochemistry, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
Amane
A
Kogure
Ikuo
I
Shiratori
Jing
J
Wang
Lewis L
LL
Lanier
Hisashi
H
Arase
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D006801:Humans; D008024:Ligands; D008546:Melanoma, Experimental; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011971:Receptors, Immunologic / Q000378:metabolism*
D008024:Ligands; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C557210:PIANP protein, human; C412263:PILRA protein, human; C548037:PILRalpha protein, mouse; C557209:Pianp protein, mouse; D011971:Receptors, Immunologic
Glycoconj J. 1998 Feb;15(2):115-30
9557871
J Virol. 2010 Oct;84(20):10773-83
20686018
J Virol. 2009 Dec;83(24):13042-5
19812165
J Immunol. 2005 Oct 1;175(7):4441-9
16177086
Biochem J. 1991 Apr 15;275 ( Pt 2):529-34
2025231
Crit Rev Biochem Mol Biol. 1998;33(3):151-208
9673446
Science. 2002 May 17;296(5571):1323-6
11950999
J Exp Med. 2004 Feb 16;199(4):525-33
14970179
FEBS Lett. 2010 Dec 15;584(24):4895-900
20828570
J Immunol. 2008 Feb 1;180(3):1686-93
18209065
Nat Immunol. 2008 May;9(5):495-502
18425106
J Biol Chem. 2002 Mar 29;277(13):11456-64
11805109
J Immunol. 2001 Aug 1;167(3):1141-4
11466327
J Biol Chem. 2008 Apr 4;283(14):8893-901
18234675
Immunology. 2009 Apr;126(4):449-57
19278418
Cell. 2008 Mar 21;132(6):935-44
18358807
Science. 2000 Oct 6;290(5489):84-9
11021804
Physiol Genomics. 2006 Nov 27;27(3):201-18
16926269
J Exp Med. 1997 Dec 15;186(12):1957-63
9396764