Cloning of two genes (LAT1,2) encoding specific L: -arabinose transporters of the L: -arabinose fermenting yeast Ambrosiozyma monospora.
We identified and characterized two genes, LAT1 and LAT2, which encode specific L: -arabinose transporters. The genes were identified in the L: -arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low L: -arabinose transport activity; however, when LAT1 or LAT2 was expressed, L: -arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the L: -arabinose transporters could not restore growth on D: -glucose, D: -fructose, D: -mannose or D: -galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for L: -arabinose.
VTT Technical Research Centre of Finland, Tietotie 2, Espoo, Fi-02044 VTT, Finland.
Ritva
R
Verho
Merja
M
Penttilä
Peter
P
Richard
D001089:Arabinose / Q000378:metabolism*; D001692:Biological Transport; D003001:Cloning, Molecular; D005285:Fermentation; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity
D009004:Monosaccharide Transport Proteins; D001089:Arabinose