Characterization and selectivity studies of molecular imprinted membranes of Puerarin using scanning electron microscopy.
Molecular-imprinted membranes of Puerarin were prepared by phase inversion technique with acrylonitrile-acrylic acid copolymer (P (AN-co-AA)). To characterize P (AN-co-AA), ubbelohde viscometer was used to measure its viscosity-molecular weight. P (AN-co-AA) with different molecular weight was used to prepare membranes. The copolymer-dimethyl sulfoxide solution with Puerarin (PU) template was coagulated in water at various temperatures. The increase in P (AN-co-AA) molecular weight and the decrease in coagulation temperature caused an increase in PU recognition property of the resultant membrane. The PU imprinted membrane prepared with P (AN-co-AA) showed good selective ability to PU. The purity of PU increased from 56.51 to 98.41 wt%. Surface and cross-section morphology of the membranes were analyzed by using scanning electron micrograph. High-performance liquid chromatography was used for the quantification of Puerarin in isolated fraction.
Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, People's Republic of China.
Peiyong
P
Qin
Jie
J
Yang
M Nazmul
MN
Karim
D000179:Acrylates / Q000032:analysis; D000181:Acrylonitrile / Q000032:analysis; D007529:Isoflavones / Q000737:chemistry*; D008566:Membranes / Q000737:chemistry* / Q000648:ultrastructure*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting / Q000379:methods*; D008970:Molecular Weight; D011108:Polymers / Q000032:analysis; D014783:Viscosity
D000179:Acrylates; D007529:Isoflavones; D008567:Membranes, Artificial; D011108:Polymers; D000181:Acrylonitrile; C033607:puerarin