Comparison of different sample treatments for the analysis of quinolones in milk by capillary-liquid chromatography with laser induced fluorescence detection.
A simple and very sensitive capillary-liquid chromatography method coupled with laser induced fluorescence detection has been developed for the simultaneous determination of seven quinolones of veterinary use in milk. Moreover, a comparison between two different sample treatments (QuEChERS and molecularly imprinted polymer, MIP) has been carried out in terms of efficiency of the extraction (number of analytes to be analysed and absence of interferences), throughput, linear dynamic range in matrix-matches calibrations, detection and quantification limits and accuracy (trueness and precision, by means of recovery assays). The results showed that the QuEChERS procedure was more efficient and faster, showing good recoveries, sensitivity and precision for all the studied compounds. Employing this proposed method, very low detection limits, between 0.4 μg/kg for danofloxacin, and 6 μg/kg for sarafloxacin, have been obtained.
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
Manuel
M
Lombardo-Agüí
Laura
L
Gámiz-Gracia
Carmen
C
Cruces-Blanco
Ana M
AM
García-Campaña
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D024841:Fluoroquinolones / Q000032:analysis; D007834:Lasers; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D024841:Fluoroquinolones; D015363:Quinolones; C068581:danofloxacin; D002939:Ciprofloxacin; C047224:sarafloxacin