Surface hydrophilic modification with well-defined glycopolymer for protein imprinting matrix.
Well-defined lactose-containing glycopolymer has been synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization with (4-cyanopentanoic acid)-4- dithiobenozoate (CAD) as chain transfer agent. The glycopolymer was introduced onto the exterior surfaces of the bovine serum albumin (BSA) imprinted polymer beads by grafting copolymerization with methyl methacrylate and ethylene glycol dimethacrylate. After alcoholysis, the hydrophilic lactose residues of glycopolymer will stretched on the surface of the MIP beads and then the hydrophilicity of the surface will be enhanced. Rebinding test shows that the glycopolymer hydrophilic modified BSA imprinted polymer presents higher performance selectivity than that of unmodified one, which means that the hydrophobic-hydrophilic balance of the imprinted polymer surface is in favor of the improvement of specific recognition property of the material.
Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Weijin Road, No. 94, Tianjin 300071, China.
Huan
H
Yang
Tian-Ying
TY
Guo
Dezhong
D
Zhou
D057927:Hydrophobic and Hydrophilic Interactions; D008863:Microspheres; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D013499:Surface Properties
D011108:Polymers