Atrazine biodegradation in the lab and in the field: enzymatic activities and gene regulation.
Atrazine is an herbicide of the s-triazine family that is used primarily as a nitrogen source by degrading microorganisms. While many catabolic pathways for xenobiotics are subjected to catabolic repression by preferential carbon sources, atrazine utilization is repressed in the presence of preferential nitrogen sources. This phenomenon appears to restrict atrazine elimination in nitrogen-fertilized soils by indigenous organisms or in bioaugmentation approaches. The mechanisms of nitrogen control have been investigated in the model strain Pseudomonas sp. ADP. Expression of atzA, atzB ad atzC, involved in the conversion of atrazine in cyanuric acid, is constitutive. The atzDEF operon, encoding the enzymes responsible for cyanuric acid mineralization, is a target for general nitrogen control. Regulation of atzDEF involves a complex interplay between the global regulatory elements of general nitrogen control and the pathway-specific LysR-type regulator AtzR. In addition, indirect evidence suggests that atrazine transport may also be a target for nitrogen regulation in this strain. The knowledge about regulatory mechanisms may allow the design of rational bioremediation strategies such as biostimulation using carbon sources or the use of mutant strains impaired in the assimilation of nitrogen sources for bioaugmentation.
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC, and Departamento de Biología Molecular e Ingeniería Bioquímica, Universidad Pablo de Olavide, Spain. fgovrom@upo.es
Fernando
F
Govantes
Odil
O
Porrúa
Vicente
V
García-González
Eduardo
E
Santero
D001280:Atrazine / Q000032:analysis / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015964:Gene Expression Regulation, Bacterial; D006540:Herbicides / Q000032:analysis / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
D001426:Bacterial Proteins; D006540:Herbicides; D012989:Soil Pollutants; D001280:Atrazine
J Bacteriol. 2005 Jan;187(1):155-67
15601699
J Bacteriol. 2002 Oct;184(19):5376-84
12218024
Appl Environ Microbiol. 1997 Mar;63(3):916-23
9055410
J Bacteriol. 2008 Jan;190(1):416-20
17965157
Mol Microbiol. 2007 Oct;66(2):410-27
17854404
J Bacteriol. 1989 Oct;171(10):5512-22
2571609
J Bacteriol. 2007 Feb;189(3):674-82
17114259
Appl Microbiol Biotechnol. 1999 Mar;51(3):375-81
10222586
Appl Environ Microbiol. 1994 Feb;60(2):613-8
16349190
Nature. 2002 Oct 31;419(6910):895-6
12410298
J Bacteriol. 1991 Feb;173(3):1363-6
1991731
Appl Environ Microbiol. 1998 Sep;64(9):3368-75
9726884
Environ Toxicol Chem. 2001 Apr;20(4):769-75
11345452
Appl Environ Microbiol. 2003 Dec;69(12):6987-93
14660340
Appl Environ Microbiol. 1994 Dec;60(12):4297-302
7811069
Appl Environ Microbiol. 1997 Mar;63(3):862-6
16535552
J Bacteriol. 2003 Feb;185(4):1245-52
12562794
Appl Environ Microbiol. 2002 Dec;68(12):5973-80
12450818
Appl Environ Microbiol. 1993 Jun;59(6):1955-9
8328812
Appl Environ Microbiol. 2003 Jun;69(6):3653-7
12788776
J Bacteriol. 2001 Apr;183(8):2405-10
11274097
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D517-21
16381924
J Mol Biol. 1997 Jul 11;270(2):125-38
9236116
Appl Environ Microbiol. 2005 Aug;71(8):4437-45
16085834
Appl Environ Microbiol. 2005 May;71(5):2214-20
15870302
Curr Opin Microbiol. 2006 Apr;9(2):167-72
16458044
J Bacteriol. 1998 Apr;180(7):1951-4
9537398
Annu Rev Microbiol. 1993;47:597-626
8257110
Appl Environ Microbiol. 2000 Aug;66(8):3134-41
10919761
Appl Environ Microbiol. 1995 May;61(5):2056-60
7646049
Appl Environ Microbiol. 1995 Jan;61(1):297-302
7887609
J Bacteriol. 1995 Oct;177(20):5748-55
7592318
Eur J Biochem. 2004 Aug;271(16):3379-88
15291815
J Bacteriol. 1998 Jan;180(1):152-8
9422605
Appl Environ Microbiol. 1995 Apr;61(4):1451-7
16534995
J Bacteriol. 2001 Oct;183(19):5684-97
11544232
J Bacteriol. 1991 Feb;173(3):1215-22
1846859
Environ Toxicol Chem. 2007 May;26(5):844-50
17521128
Environ Sci Technol. 2004 Jan 15;38(2):632-7
14750741
J Microbiol Methods. 2004 Jan;56(1):3-15
14706746
Appl Environ Microbiol. 2004 Jul;70(7):4402-7
15240330
Environ Health Perspect. 2003 Apr;111(4):568-75
12676617
J Mol Biol. 1995 Nov 10;253(5):691-702
7473744
Biodegradation. 2002;13(1):3-10
12222953
Biochem J. 1985 Oct 1;231(1):25-30
3904735
Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14674-9
11121068
Appl Environ Microbiol. 2002 Sep;68(9):4672-5
12200330
Proc Natl Acad Sci U S A. 1990 Jul;87(14):5504-8
2164685
Appl Environ Microbiol. 1998 Jun;64(6):2323-6
9603862
Appl Microbiol Biotechnol. 2002 Jan;58(1):39-45
11831474
Appl Environ Microbiol. 1999 Aug;65(8):3512-7
10427042
Appl Environ Microbiol. 1995 May;61(5):2061-5
7646050
Appl Environ Microbiol. 2000 Jul;66(7):2773-82
10877767
J Bacteriol. 2005 Jun;187(11):3731-8
15901697
FEMS Microbiol Ecol. 2001 Jul;36(2-3):211-222
11451526
Trends Microbiol. 2000 Apr;8(4):172-9
10754576
J Bacteriol. 1996 Aug;178(16):4894-900
8759853
EMBO J. 2001 Jan 15;20(1-2):1-11
11226149
Appl Environ Microbiol. 2004 Apr;70(4):1907-12
15066779
Nat Rev Microbiol. 2005 Feb;3(2):105-18
15685222