Prognostic value of serum LP-PLA2 and hs-CRP in unstable atherosclerotic plaques.
To evaluate the prognostic value of LP-PLA2 and hs-CRP to the stability of atherosclerotic plaques. Forty-eight New Zealand White rabbits were randomly divided into control group, stable plaque group, P53 group, and P53 + drug group. Rabbits in the control group were fed a regular diet. Rabbits in stable plaque group, P53 group, and P53 + drug group underwent balloon-induced arterial wall injury and then were fed a diet of 1% cholesterol. Then the rabbits in the P53 group and P53 + drug group underwent Ad5-CMV P53 transfection in the 10th week; The P53 + drug group underwent pharmacologic triggering with Russell's viper venom (RVV) and histamine in 24 h and 48 h before euthanized. Intravascular ultrasound (IVUS) was used before sacrificing of the animal. In the 0 week and 12th week, rabbits underwent fast blood collection from the medium-sized artery of the ears, and the serum LP-PLA2 and hs-CRP level was determined. The animal altherosclerotic (AS) model was successfully gained and the rules of serum LP-PLA2 and hs-CRP level in instable plaque were discovered: serum LP-PLA2 in P53 group and P53 + drug group were significantly different from the control group and the stable group, while hs-CRP failed to differ between the control group and the stable group and succeeded in different degrees of unstable plague. The relationship analysis of serum and IVUS results revealed LP-PLA2 might predict an instability of plaque. Combining serum Lp-PLA2 and hs-CRP has higher specificity in predicting the vulnerability of the plaque.
Department of Cardiology, The Second Hospital of Shandong University, Jinan, China.
Yimeng
Y
Du
Fengtao
F
Wei
Zhaoqiang
Z
Dong
Junni
J
Liu
Weidong
W
Jiang
Qinghua
Q
Lu
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D000818:Animals; D001483:Base Sequence; D002097:C-Reactive Protein / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D017931:DNA Primers / Q000235:genetics; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D016158:Genes, p53; D058226:Plaque, Atherosclerotic / Q000097:blood* / Q000000981:diagnostic imaging / Q000209:etiology / Q000473:pathology; D011379:Prognosis; D011817:Rabbits; D014162:Transfection; D014463:Ultrasonography
D002791:Cholesterol, Dietary; D017931:DNA Primers; D002097:C-Reactive Protein; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase