Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E.
Bacterial membranes constitute the first physical barrier against different environmental stresses. Pseudomonas putida DOT-T1E accumulates cyclopropane fatty acids (CFAs) in the stationary phase of growth. In this strain the cfaB gene encodes the main cyclopropane synthase responsible of the synthesis of CFAs, and its expression is mediated by RNA polymerase with sigma factor σ(38). We generated a cfaB mutant of P. putida DOT-T1E and studied its response to solvents, acid pH and other stress conditions such as temperature changes, high osmolarity and the presence of antibiotics or heavy metals in the culture medium. A CfaB knockout mutant was more sensitive to solvent stress than the wild-type strain, but in contrast to Escherichia coli and Salmonella enterica, the P. putida cfaB mutant was as tolerant to acid shock as the wild-type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents.
Environmental Protection Department, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/Profesor Albareda 1, 18008-Granada, Spain.
Cecilia-Vanesa
CV
Pini
Patricia
P
Bernal
Patricia
P
Godoy
Juan-Luis
JL
Ramos
Ana
A
Segura
D000143:Acids / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003521:Cyclopropanes / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000378:metabolism / Q000494:pharmacology*; D016958:Pseudomonas putida / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological
D000143:Acids; D001426:Bacterial Proteins; D003521:Cyclopropanes; D005227:Fatty Acids; D009930:Organic Chemicals; C028775:cyclopropane fatty acids; D008780:Methyltransferases; C019964:cyclopropane synthetase
J Bacteriol. 1995 Jul;177(14):3911-6
7608060
J Bacteriol. 2003 Aug;185(16):4755-63
12896994
Annu Rev Microbiol. 2002;56:743-68
12142492
J Bacteriol. 1998 Jul;180(13):3323-9
9642183
Appl Environ Microbiol. 1996 Mar;62(3):1129-32
16535264
Microbiol Mol Biol Rev. 1997 Dec;61(4):429-41
9409147
Proc Natl Acad Sci U S A. 1974 Feb;71(2):522-5
4360948
Microbiology (Reading). 2005 Jan;151(Pt 1):209-218
15632439
Environ Microbiol. 2007 Jun;9(6):1550-61
17504492
J Biol Chem. 1997 Feb 14;272(7):3887-90
9020089
Gene. 1984 Sep;29(3):303-13
6237955
Appl Environ Microbiol. 1992 Jun;58(6):1847-52
1622260
J Bacteriol. 1996 Oct;178(20):6056-8
8830706
Microbiol Rev. 1995 Jun;59(2):201-22
7603409
Biosci Biotechnol Biochem. 2004 Feb;68(2):317-23
14981294
Gene. 1995 Dec 1;166(1):175-6
8529885
Appl Environ Microbiol. 2003 May;69(5):2831-41
12732555
Environ Microbiol. 2002 Dec;4(12):799-808
12534463
Appl Environ Microbiol. 2001 Sep;67(9):4338-41
11526042
J Bacteriol. 1999 Sep;181(18):5693-700
10482510
Appl Environ Microbiol. 2005 Apr;71(4):1915-22
15812020
Can J Biochem Physiol. 1959 Aug;37(8):911-7
13671378
Biotechniques. 1998 Dec;25(6):954-6, 958
9863047
Biochim Biophys Acta. 1993 Nov 16;1216(2):227-36
8241263
J Bacteriol. 2001 Apr;183(7):2178-86
11244055
Int J Food Microbiol. 1997 Jul 22;37(2-3):163-73
9310851
J Mol Biol. 2000 Oct 27;303(3):371-82
11031114
Environ Microbiol. 1999 Jun;1(3):191-8
11207738
Gene. 1999 May 17;232(1):69-76
10333523
Mol Microbiol. 1999 Jul;33(2):249-59
10411742
Environ Microbiol. 2007 May;9(5):1135-45
17472630
J Biol Chem. 2006 Apr 28;281(17):11981-91
16495222
Appl Environ Microbiol. 1984 Jan;47(1):193-4
6696415
J Bacteriol. 1998 Jul;180(14):3692-6
9658016
J Bacteriol. 2000 Aug;182(15):4288-94
10894739
Plant Mol Biol. 1987 Jan;9(1):27-39
24276795
Biochim Biophys Acta. 1996 Oct 29;1286(3):225-45
8982284
J Biol Chem. 1998 Jan 2;273(1):85-91
9417051
Appl Environ Microbiol. 2005 Aug;71(8):4191-8
16085802
Appl Environ Microbiol. 2006 Jan;72(1):472-7
16391080
J Bacteriol. 2001 Jul;183(13):3967-73
11395460
J Bacteriol. 1989 Dec;171(12):6782-90
2687253
Environ Microbiol. 2007 Jul;9(7):1658-64
17564601