Saponins from Plumeria acuminata Ait induce cell growth inhibition and apoptosis of oral squamous carcinoma cells.
OBJECTIVE
to investigate the cytotoxic and apoptotic effects of saponins from Plumeria acuminata Ait on oral squamous carcinoma cells (OSCC).


METHODS
OSCC cells seeded at 2 × 104 cells/well in a 96-well plate were treated with saponins from P. acuminata Ait and cisplatin in various concentrations for 24 h. Trypan blue dye exclusion assay and ethidium bromide/acridine orange were used to evaluate their cytotoxic and apoptotic effects on the cells, respectively.


RESULTS
the results showed that both saponins and cisplatin had cytotoxic and apoptotic effects on OSCC cells.


CONCLUSIONS
saponins from P. acuminata Ait may be potential anti-cancer agents for OSCC.
Oral Medical Department, Faculty of Dentistry, University of Gadjah Mada, Yogyakarta, Indonesia. drghendri@ugm.ac.id
Hendri
H
Susanto
Nanang
N
Fakhrudin
Yosi Bayu
YB
Murti
Supriatno
Widowati
W
Siswomiharjo
D000970:Antineoplastic Agents / Q000008:administration & dosage; D029062:Apocynaceae; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000008:administration & dosage*; D006801:Humans; D020128:Inhibitory Concentration 50; D009062:Mouth Neoplasms / Q000473:pathology; D010936:Plant Extracts / Q000008:administration & dosage*; D018547:Plant Stems; D012503:Saponins / Q000008:administration & dosage*
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; D010936:Plant Extracts; D012503:Saponins; D002945:Cisplatin