Effect of fluocinolone acetonide on human dental pulp cells: cytotoxicity, proliferation, and extracellular matrix formation.
BACKGROUND
The goal of vital pulp therapy is to maintain pulp vitality and function. Fluocinolone acetonide is a potent topical glucocorticoid used in the treatment of skin disorders and oral lesions that could possibly be used to resolve inflammation and stimulate the healing process of inflamed dental pulp. The purpose of this study was to investigate the effects of fluocinolone acetonide (0.1-50 μmol/L) on cytotoxicity, cell proliferation, and fibronectin and type I collagen synthesis in human dental pulp cells (HDPCs).


METHODS
HDPCs were prepared from freshly extracted human third molars. MTT assay was used to determine toxicity and cell proliferation. Western blot analysis was performed to detect fibronectin and type I collagen synthesis.


RESULTS
Low concentrations of fluocinolone acetonide were not only nontoxic but also significantly stimulated cell proliferation (P < .05). Fluocinolone acetonide significantly stimulated fibronectin and type I collagen synthesis (P < .05).


CONCLUSIONS
Low concentrations (0.1-10 μmol/L) of fluocinolone acetonide might have the potential to stimulate healing of inflamed dental pulp.
Department of Endodontics, Chulalongkorn University, Bangkok, Thailand.
Wannakanok
W
Muincharern
Phumisak
P
Louwakul
Prasit
P
Pavasant
Veera
V
Lertchirakarn
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000187:drug effects / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000187:drug effects / Q000378:metabolism; D005446:Fluocinolone Acetonide / Q000008:administration & dosage*; D005938:Glucocorticoids / Q000008:administration & dosage*; D006801:Humans; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000008:administration & dosage
D024042:Collagen Type I; D005353:Fibronectins; D005938:Glucocorticoids; D057872:Pulp Capping and Pulpectomy Agents; D012388:Root Canal Irrigants; D005446:Fluocinolone Acetonide