Development and validation of I-DNA1: a 15-Loci multiplex system for identity testing.
This paper presents a system for the multiplex amplification of 15 loci, known as I-DNA1, which combines mini and midiSTR technology, with amplicon sizes ranging from 49 to 297 bp. I-DNA1 analyses all the STR loci included in the CODIS and the Interpol Standard Set of loci, nine of the ten European core loci and seven of the eight German core loci, making it suitable for use in identifying humans. Moreover, its high sensitivity and the small size of its amplicons mean that I-DNA1 is potentially highly useful for analysing highly degraded and/or very small DNA samples.
Grupo BIOMICs, Banco de ADN UPV/EHU, Universidad del País Vasco UPV/EHU, CIEA Centro de Investigación y Estudios Avanzados Lucio Lascaray, Avda. Miguel de Unamuno 3, Vitoria-Gasteiz, Álava, Spain.
A
A
Odriozola
J M
JM
Aznar
D
D
Celorrio
M L
ML
Bravo
J J
JJ
Builes
J F
JF
Val-Bernal
Marian M
MM
de Pancorbo
D016172:DNA Fingerprinting / Q000331:legislation & jurisprudence* / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D018732:Forensic Anthropology / Q000331:legislation & jurisprudence* / Q000379:methods*; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000473:pathology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data*; D009206:Myocardium / Q000473:pathology; D011180:Postmortem Changes; D011933:Reagent Kits, Diagnostic / Q000706:statistics & numerical data*; D015203:Reproducibility of Results; D013030:Spain
D011933:Reagent Kits, Diagnostic
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