Factor V Leiden and prothrombin gene mutations in Egyptian cases with unexplained recurrent pregnancy loss.
BACKGROUND
Thrombophilias have been suggested as a possible cause of recurrent pregnancy loss (RPL).


OBJECTIVE
Testing for the association of factor V Leiden (FVL) and prothrombin (FII) mutations with RPL among cases from the Nile Delta region of Egypt.


METHODS
Participants included 72 cases having a history of two or more events of unexplained RPL and 70 controls with a good obstetric history. Detection of FVL (G1691A) and FII (G20210A) mutations was carried out using PCR with sequence specific primers.


RESULTS
Cases showed a significantly higher frequency of FVL GA (OR = 21·38, P<0·0001) and FII GA (OR = 36·7, P<0·0001) genotypes. Cases with two or more risk factors had significant higher frequency of both mutant genotypes, while no significant difference could be elicited related to primary or secondary infertility, number of fetal losses, or phase of pregnancy loss.


CONCLUSIONS
Screening for thrombophilic mutations may help in the prevention of unexplained RPL.
Genetics Unit, Mansoura University Children Hospital, Egypt. settin60@gmail.com
Ahmad
A
Settin
RababAbo
R
Alkasem
Ehab
E
Ali
Rizk
R
ElBaz
Abdel Megid
AM
Mashaley
D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D004534:Egypt; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D009154:Mutation; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000235:genetics*; D011516:Prothrombin / Q000235:genetics*; D012307:Risk Factors; D019851:Thrombophilia / Q000235:genetics*; D055815:Young Adult
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin