A new HPTLC densitometric method for analysis of swertiamarin in Enicostemma littorale and commercial formulations.
A high-performance thin layer chromatographic densitometric method for the analysis of swertiamarin in 60% methanolic extract of Enicostemma littorale and commercial formulations has been developed and validated in this study. Swertiamarin was separated on aluminium-backed silica gel 60 F₂₅₄ plates using ethyl acetate : methanol : water (77 : 15 : 8)% v/v as the mobile phase. This system was found to give a compact spot of swertiamarin at R(f) value 0.36 ± 0.01. The limit of detection and limit of quantification were found to be 31.25 and 103.12 ng spot⁻¹, respectively. The proposed method was employed with a high degree of precision and accuracy for the estimation of swertiamarin in methanolic extract of Enicostemma littorale and in commercial formulations.
Department of Pharmacognosy, College of Pharmacy, Al-Kharj University, Al-Kharj, Kingdom of Saudi Arabia. prawez_pharma@yahoo.com
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Alam
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Ali
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Singh
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Shakeel
D002138:Calibration; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry / Q000379:methods*; D029965:Gentianaceae / Q000737:chemistry*; D057888:Iridoid Glucosides / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011753:Pyrones / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
D057888:Iridoid Glucosides; D010936:Plant Extracts; D011753:Pyrones; C013270:swertiamarin