IGF-II inhibitory DNAzymes inhibit the invasion and migration of hepatocarcinoma cells.
Metastasis and recurrence are the biggest obstacles to enhance the efficacy of surgical resection as a cure for hepatocellular carcinoma which is the second most deadly cancer in China. Here, we had showed that two DNAzymes (DRz1 and DRz2) targeted to IGF-II could inhibit invasion, motility and migration of SMMC-7721 cells in vitro. DRz1 was transfected into SMMC-7721 cells and the results were shown that IGF-II expression level dramatically reduced. Meanwhile, DRz1 effectively inhibited adhesion between SMMC-7721 cells with the extracellular matrix and Fb cells comparing to those untreated or transfected with inactive DRz (P<0.05, ANOVA). Furthermore, vascular endothelial growth factor and matrix metalloproteinases were down-regulated in DRz1 treated cells. These results may help to identify novel therapeutic molecules targeting hepatocellular carcinomas.
Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University, Room 226, Life Science Building, Qianjin Street 2699, Changchun 130012, China.
Min
M
Zhang
Zhang
Z
Min
Hang
H
Zhao
Fangyue
F
Luo
Su
S
Luo
Luo
L
Su
Wei
W
Shi
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D021881:DNA, Catalytic / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000037:antagonists & inhibitors*
D021881:DNA, Catalytic; D007335:Insulin-Like Growth Factor II