Growth and poliovirus production of Vero cells on a novel microcarrier with artificial cell adhesive protein under serum-free conditions.
A microcarrier is used for the three-dimensional (3D) culture of adhesion-dependent mammalian cells. We developed a novel microcarrier by binding ProNectin F, an artificial cell adhesive protein synthesized by genetically engineered Escherichia coli to a polyacrylic superabsorbent polymer. The microcarrier is characterized by containing no animal-derived components. The serum-free culture of Vero cells for vaccine production using the microcarrier increased the number of Vero cells by approximately 30% compared with the existing dextran beads coated with porcine Type I collagen, which resulted in approximately a 30% to 40% increase in the infectivity titer of the Sabin 2 strain of poliovirus. These results suggested that the developed microcarrier should be unprecedented in permitting high-yield vaccine production by means of a serum-free culture.
Department of Microbiology, School of Medicine, Iwate Medical University, Morioka, Iwate, Japan. m.kurokawa@sanyo-chemical.com
Masato
M
Kurokawa
Shigehiro
S
Sato
D000818:Animals; D019149:Bioreactors; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D002522:Chlorocebus aethiops; D016895:Culture Media, Serum-Free; D003911:Dextrans; D008863:Microspheres; D017955:Poliovirus / Q000254:growth & development*; D011108:Polymers / Q000737:chemistry; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*
D015815:Cell Adhesion Molecules; D016895:Culture Media, Serum-Free; D003911:Dextrans; D011108:Polymers