Identification and characterization of gene expression involved in the coloration of cichlid fish using microarray and qRT-PCR approaches.
It has been suggested that speciation on the basis of sexual selection is an important mechanism for the generation of new species for East African cichlids, where male body coloration is one of the major discriminatory factors used by females in mate choice. To gain insight into the molecular basis of cichlid coloration, we studied the Lake Malawi cichlid Pseudotropheus saulosi, comparing transcription in the bright blue skin of males to the yellow skin of females. Our cDNA microarray experiments identified 46 clones that exhibited expression differences between the two sexes, of which five were confirmed to be differentially expressed by relative quantitative real-time PCR (qRT-PCR). This gene list includes a representative from the endosomal-to-Golgi vesicle trafficking pathway, Coatomer protein complex, subunit zeta-1 (Copz-1), which is known to be a critical determinant of pigmentation in humans and zebrafish. With the support of microscopic images of the skin of these specimens, we interpret the transcriptional differences between the blue males and yellow females. Here, we provide insight into the putative functional diversification of genes involved in the coloration of cichlids and by extension, on the evolution of coloration in teleost fish.
Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Universitätsstr 10, 78457 Constance, Germany.
Helen M
HM
Gunter
Céline
C
Clabaut
Walter
W
Salzburger
Axel
A
Meyer
D000818:Animals; D023681:Cichlids / Q000235:genetics*; D020756:Coatomer Protein / Q000235:genetics; D024042:Collagen Type I / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010860:Pigments, Biological / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012880:Skin Pigmentation / Q000235:genetics*; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics
D020756:Coatomer Protein; D024042:Collagen Type I; D010860:Pigments, Biological; D044763:Ubiquitin-Conjugating Enzymes
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