Direct diagnosis of Leishmania species on serosity materials punctured from cutaneous leishmaniasis patients using PCR-RFLP.
This study was aimed at identifying the Leishmania species using serosity materials punctured from skin lesions of cutaneous leishmaniasis (CL) patients by using internal transcribed spacer1 (ITS1) polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). We used the PCR-RFLP on 60 parasitological confirmed CL patients who referred to leishmaniasis laboratory from the School of Public Health, Tehran University of Medical Sciences. The PCR-RFLP could correctly detect 51 Leishmania species of the 60 confirmed positive specimens, where all the other 10 parasitological (microscopy and culture) negative samples that were prepared from other bacterial- and fungal-infected lesions had negative results. The results also revealed that Leishmania major was the dominant species (53.3%). This study suggests that the PCR-RFLP assay with serosity materials punctured from CL patients using Hae III enzyme is useful for the rapid identification of Leishmania species.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Homa
H
Hajjaran
Farzaneh
F
Vasigheh
Mehdi
M
Mohebali
Sasan
S
Rezaei
Setareh
S
Mamishi
Soroure
S
Charedar
D000293:Adolescent; D000328:Adult; D054458:Amplified Fragment Length Polymorphism Analysis; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification*; D018320:Leishmania major / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D012680:Sensitivity and Specificity; D012867:Skin / Q000469:parasitology*; D055815:Young Adult
Trans R Soc Trop Med Hyg. 2001 May-Jun;95(3):239-43
11490989
Clin Infect Dis. 2003 Dec 1;37(11):e149-53
14614687
J Clin Microbiol. 2006 Apr;44(4):1435-9
16597873
J Infect Dis. 2004 Mar 15;189(6):1035-43
14999607
Trans R Soc Trop Med Hyg. 2007 Apr;101(4):368-71
17011005
Lancet. 1999 Oct 2;354(9185):1191-9
10513726
Mem Inst Oswaldo Cruz. 2009 Feb;104(1):62-6
19274378
Curr Opin Infect Dis. 2003 Apr;16(2):97-101
12734442
Exp Parasitol. 2007 Apr;115(4):339-43
17083934
Adv Parasitol. 2006;61:223-74
16735166
J Clin Microbiol. 2007 Jan;45(1):21-5
17093038
Trans R Soc Trop Med Hyg. 1999 Nov-Dec;93(6):606-7
10717745
Am J Trop Med Hyg. 2007 May;76(5):896-901
17488912
J Infect Dev Ctries. 2009 Sep 15;3(2):115-22
19755741
J Clin Microbiol. 2006 Feb;44(2):459-67
16455899
Int J Parasitol. 2007 Feb;37(2):149-60
17027989
Trans R Soc Trop Med Hyg. 1996 Mar-Apr;90(2):133-5
8761571
J Antimicrob Chemother. 2004 Feb;53(2):158-66
14729756
Diagn Microbiol Infect Dis. 2003 Sep;47(1):349-58
12967749