A facile method to increase titers of miRNA-encoding retroviruses by inhibition of the RNaseIII enzyme Drosha.
Division of Molecular Immunology, Department of Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.
Andreas
A
Brandl
Jürgen
J
Wittmann
Hans-Martin
HM
Jäck
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000821:virology; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D041681:NIH 3T3 Cells; D034741:RNA, Small Interfering / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D043244:Ribonuclease III / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic / Q000379:methods*; D014779:Virus Replication / Q000235:genetics*
D035683:MicroRNAs; C543896:Mirn106 microRNA, mouse; C529101:Mirn150 microRNA, mouse; D034741:RNA, Small Interfering; C513400:Drosha protein, mouse; D043244:Ribonuclease III