Selective enhancement of nucleases by polyvalent DNA-functionalized gold nanoparticles.
We demonstrate that polyvalent DNA-functionalized gold nanoparticles (DNA-Au NPs) selectively enhance ribonuclease H (RNase H) activity while inhibiting most biologically relevant nucleases. This combination of properties is particularly interesting in the context of gene regulation, since high RNase H activity results in rapid mRNA degradation and general nuclease inhibition results in high biological stability. We have investigated the mechanism of selective RNase H activation and found that the high DNA density of DNA-Au NPs is responsible for this unusual behavior. This work adds to our understanding of polyvalent DNA-Au NPs as gene regulation agents and suggests a new model for selectively controlling protein-nanoparticle interactions.
Department of Chemistry and International Institute of Nanotechnology, Northwestern University, Evanston, Illinois 60208, United States.
Andrew E
AE
Prigodich
Ali H
AH
Alhasan
Chad A
CA
Mirkin
D004247:DNA / Q000737:chemistry*; D004720:Endonucleases / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D016914:Ribonuclease H / Q000737:chemistry
D006046:Gold; D004247:DNA; D004720:Endonucleases; C091139:ribonuclease HII; D016914:Ribonuclease H
Nature. 2009 Nov 26;462(7272):461-4
19940916
Chem Rev. 2005 Apr;105(4):1547-62
15826019
Angew Chem Int Ed Engl. 2010 Jun 21;49(27):4589-92
20486143
Annu Rev Biophys Biomol Struct. 2007;36:79-105
17477836
Nature. 1996 Aug 15;382(6592):607-9
8757129
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10763-7
1279694
Mol Pharm. 2009 Nov-Dec;6(6):1934-40
19810673
Nature. 2008 Jan 31;451(7178):553-6
18235497
Science. 2003 Sep 26;301(5641):1884-6
14512622
Nature. 2008 Jan 31;451(7178):549-52
18235496
Science. 2006 May 19;312(5776):1027-30
16709779
Angew Chem Int Ed Engl. 2001 Nov 19;40(22):4128-4158
29712109
Nano Lett. 2009 Jan;9(1):308-11
19099465
Bioconjug Chem. 2008 May;19(5):1009-16
18393455
Annu Rev Med. 2004;55:61-95
14746510
J Am Chem Soc. 2005 Sep 21;127(37):12754-5
16159241
Nature. 1990 Sep 20;347(6290):306-9
1698262
Trends Biotechnol. 2003 Feb;21(2):74-81
12573856
ACS Nano. 2009 Aug 25;3(8):2147-52
19702321
J Am Chem Soc. 2007 Dec 19;129(50):15477-9
18034495
Nature. 1996 Aug 15;382(6592):609-11
8757130
Bioconjug Chem. 2006 Sep-Oct;17(5):1178-83
16984126
Nano Lett. 2007 Dec;7(12):3818-21
17997588
J Biol Chem. 2003 Dec 12;278(50):49860-7
14506260
Adv Drug Deliv Rev. 2000 Oct 31;44(1):3-21
11035194
Anal Chem. 2005 Sep 1;77(17):5735-41
16131089
J Am Chem Soc. 2008 May 7;130(18):5883-5
18407639
J Am Chem Soc. 2009 Feb 18;131(6):2072-3
19170493
Angew Chem Int Ed Engl. 2010 Apr 26;49(19):3280-94
20401880
Nucleic Acids Res. 2004 Jun 03;32(10):3040-52
15178741
J Am Chem Soc. 2003 Jun 4;125(22):6642-3
12769568
J Am Chem Soc. 2010 Aug 11;132(31):10638-41
20681682
Angew Chem Int Ed Engl. 2010 Mar 8;49(11):1924-42
20183835
Chembiochem. 2007 Apr 16;8(6):603-6
17373017