Identification of a linear epitope on the capsid protein of classical swine fever virus.
The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids (61)TQDGLYHNKN(70) of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication.
Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, 150001 Harbin, Heilongjiang, China.
Xin
X
Zhang
Jia
J
Xu
Yuan
Y
Sun
Su
S
Li
Na
N
Li
Shuo
S
Yang
Fan
F
He
Jun-Hua
JH
Huang
Li-Jun
LJ
Ling
Hua-Ji
HJ
Qiu
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D013552:Swine
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins
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