Labeling of Luciferase/eGFP-expressing bone marrow-derived stromal cells with fluorescent micron-sized iron oxide particles improves quantitative and qualitative multimodal imaging of cellular grafts in vivo.
OBJECTIVE
Development of multimodal imaging strategies is currently of utmost importance for the validation of preclinical stem cell therapy studies.


METHODS
We performed a combined labeling strategy for bone marrow-derived stromal cells (BMSC) based on genetic modification with the reporter genes Luciferase and eGFP (BMSC-Luc/eGFP) and physical labeling with blue fluorescent micron-sized iron oxide particles (MPIO) in order to unambiguously identify BMSC localization, survival, and differentiation following engraftment in the central nervous system of mice by in vivo bioluminescence (BLI) and magnetic resonance imaging and postmortem histological analysis.


RESULTS
Using this combination, a significant increase of in vivo BLI signal was observed for MPIO-labeled BMSC-Luc/eGFP. Moreover, MPIO labeling of BMSC-Luc/eGFP allows for the improved identification of implanted cells within host tissue during histological observation.


CONCLUSIONS
This study describes an optimized labeling strategy for multimodal stem cell imaging resulting in improved quantitative and qualitative detection of cellular grafts.
BioImaging Laboratory, University of Antwerp, Antwerp, Belgium.
Nathalie
N
De Vocht
Irene
I
Bergwerf
Greetje
G
Vanhoutte
Jasmijn
J
Daans
Geofrey
G
De Visscher
Shyama
S
Chatterjee
Patrick
P
Pauwels
Zwi
Z
Berneman
Peter
P
Ponsaerts
Annemie
A
Van der Linden
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D005290:Ferric Compounds / Q000737:chemistry*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008156:Luciferases / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D010316:Particle Size; D013194:Staining and Labeling; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation
D005290:Ferric Compounds; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D008156:Luciferases
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