Effect of interleukin-1β on the expression of tight junction proteins in the culture of HaCaT keratinocytes.
We studied the effect of IL-1β on the expression of tight junction proteins (occludin and claudins) in cultured HaCaT keratinocytes and changes of transepithelial resistance. Addition of IL-1β had little effect on transepithelial resistance, increased the expression of claudin-1, and did not modify the expression of occludin. In other tissues, IL-1β also increases claudin-1 expression, but significantly decreases occludin expression. These changes are accompanied by the reduction of transepithelial resistance. The IL-1β-induced increase in the expression of claudin-1 in cultured HaCaT keratinocytes simulates the appearance of claudin-1 at the early stage of skin wound healing. It is accompanied by an increase in IL-1β concentration in the wound fluid.
Department of General Physiology, Biological and Soil Faculty, St. Petersburg State University, Russia. doktorat@mail.ru
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Rozlomiy
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Markov
D015153:Blotting, Western; D057167:Claudins / Q000378:metabolism*; D003720:Densitometry; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D015603:Keratinocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D062793:Occludin; D018709:Statistics, Nonparametric; D019108:Tight Junctions / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology*
D057167:Claudins; D053583:Interleukin-1beta; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin