Novel approach to improve the detection of colchicine via online coupling of ionic liquid-based single-drop microextraction with capillary electrophoresis.
A novel approach based on ionic liquid-single-drop microextraction (IL-SDME) online coupling with capillary electrophoresis (CE) was used to determine a toxic alkaloid--colchicine. The IL-SDME procedure was optimized by extraction solvent, drop volume controlling, sample volume and pH, extraction time, and ionic strength. Under optimum conditions, enrichment factor was as much as 41-fold with a relative standard deviation of 2.8% (n = 3). Linear range of response was observed from 1 to 100 μg/mL, with detection limit of 0.25 μg/mL and correlation coefficient (R(2) ) of 0.9994. The extraction of colchicine from spiked Lanzhou lily sample was performed and obtaining good result with an average recovery rate of 102.4 and 98.8% at 5 and 50 μg/mL, respectively. Comparing with the previous methods, IL-SDME-CE is really a convenient, economical, and environmentally benign way for determining colchicine.
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, PR China.
Qing
Q
Wang
Hongdeng
H
Qiu
Jing
J
Li
Haifeng
H
Han
Xia
X
Liu
Shengxiang
S
Jiang
D003078:Colchicine / Q000032:analysis* / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D052578:Ionic Liquids; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
D052578:Ionic Liquids; D003078:Colchicine