Further interest of miniexon multiplex PCR for a rapid typing of Trypanosoma cruzi DTU groups.
In order to validate a rapid typing of Trypanosoma cruzi DTUs, the miniexon multiplex PCR was tested for the first time, on a large and diversified sample of 70 strains belonging to all current DTUs (TcI to TcVI). Three DTU groups have been distinguished by specific PCR molecular weight, TcI (200bp), TcII, V, VI (250bp) and TcIII and IV (150bp) with no incorrect grouping. These groups are epidemiologically and genetically relevant; moreover the method is easy and cheap and allows direct identification of parasites from triatomine faeces.
Institut de Recherche pour le Développement, Society and Health Department, MIVEGEC, University of Montpellier - CNRS 5290 - IRD 224, Representation in Bolivia, Av Hernando Siles N°5290, CP 9214, La Paz, Bolivia. aliagaclau@yahoo.es
Claudia
C
Aliaga
Simone Frédérique
SF
Brenière
Christian
C
Barnabé
D002489:Central America; D003710:Demography; D009656:North America; D016133:Polymerase Chain Reaction / Q000379:methods*; D013020:South America; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics*