Local CD4+, CD8+ and CD56+ reactions to lung cancer in regard to pathohistological type and clinical stage.
OBJECTIVE
To determinate the difference of abundance of CD4+, CD8+ and CD56+ bronchoalveolar fluid's lymphocytes and their subpopulations between non- and small cell lung cancer. Also, the differences of abundance of examined lymphocytes were compared between main clinical stages of lung cancer.


METHODS
Mini-bronchoalveolar lavate was taken from lungs of 55 patients with cancer. After laboratory processing and adding CD3, CD4, CD8, CD27, CD28 and CD56 antibody, the material was analysed by flow cytometer. Results of Mini-BAL for non- and small cell lung cancer were compared, as well as the different clinical stages of the disease.


RESULTS
Immature and regulatory forms of lymphocytes are more activated, while mature and activated forms are less activated in small cell lung cancer compared to non small type. With an increase of the clinical stage of disease, immunological reaction of T lymphocytes is better expressed because of increasing of abundance of immature and regulatory forms of different subpopulations of lymphocytes.


CONCLUSIONS
All components of local CD4+ and CD8+ T lymphocyte, as well as NK and NKT cells response were more activated in lungs with small cell lung cancer, and these reactions were more expressed with an increase in the clinical stage.
Policlinic for Pulmonary Diseases, Medical Centre of Tuzla, Bosnia and Herzegovina. edinjusufovic@yahoo.de
Edin
E
Jusufovic
Ermina
E
Iljazovic
Mitja
M
Kosnik
Dragan
D
Keser
Peter
P
Korosec
Edin
E
Zukic
Besim
B
Prnjavorac
Rifat
R
Sejdinović
Ekrem
E
Ajanović
D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D019002:CD56 Antigen / Q000032:analysis*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D055611:Natural Killer T-Cells / Q000276:immunology; D055752:Small Cell Lung Carcinoma / Q000276:immunology / Q000473:pathology
D019002:CD56 Antigen