Antibody-based multiplex analysis of structurally closely related chiral molecules.
Four class-specific and stereoselective antibodies were labeled with different fluorophores to enable simultaneous quantification of the enantiomers of phenylalanine and phenyllactic acid. Using fluorescence immunoassays and microarrays, sensitive detection of each analyte was possible even in the presence of a large excess of the other structurally similar molecules.
Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115, USA.
Tigabu
T
Kassa
L Pauli
LP
Undesser
Heike
H
Hofstetter
Oliver
O
Hofstetter
D005455:Fluorescent Antibody Technique / Q000379:methods*; D019344:Lactic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D019344:Lactic Acid; D010649:Phenylalanine