Low red blood cell levels of deglycating enzymes in colorectal cancer patients.
OBJECTIVE
To investigate Glyoxalase I and fructosamine-3-kinase (FN3K) activity in red blood cells from patients with colorectal adenomas and cancer.


METHODS
Thirty three consecutive subjects with one or more histologically confirmed colorectal adenomatous polyps, 16 colorectal cancer patients and a group of 11 control subjects with normal colonoscopy were included in the study. Glyoxalase I and FN3K activities were measured in red blood cells using a spectrophotometric and radiometric assay, respectively.


RESULTS
A significant reduction in both Glyoxalase I and FN3K activity was detected in patients with tumors compared to patients with adenomas and the controls. Erythrocyte Glyoxalase I activity in colorectal cancer was approximately 6 times lower than that detected in patients with adenoma (0.022 ± 0.01 mmol/min per milliliter vs 0.128 ± 0.19 mmol/min per milliliter of red blood cells, P = 0.003, Tukey's test). FN3K activity in red blood cells from patients with colon cancer was approximately 2 times lower than that detected in adenoma patients (19.55 ± 6.4 pmol/min per milliliter vs 38.6 ± 31.7 pmol/min per milliliter of red blood cells, P = 0.04, Tukey's test).


CONCLUSIONS
These findings suggest that deglycating enzymes may be involved in the malignant transformation of colon mucosa.
Maria Notarnicola, Maria Gabriella Caruso, Valeria Tutino, Laboratory of Biochemistry, National Institute for Digestive Diseases, 70013 Castellana G, Bari, Italy.
Maria
M
Notarnicola
Maria Gabriella
MG
Caruso
Valeria
V
Tutino
Vito
V
Guerra
Giovanni
G
Misciagna
D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000097:blood* / Q000201:enzymology* / Q000473:pathology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000097:blood*
D017853:Phosphotransferases (Alcohol Group Acceptor); C414347:fructosamine-3-kinase; D007791:Lactoylglutathione Lyase
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