Is the photoactive yellow protein a UV-B/blue light photoreceptor?
UV light below 300 nm is shown to generate the first photocycle intermediate in the blue light photoreceptor Photoactive Yellow Protein. Fluorescence and ultrafast transient absorption measurements indicate two excitation pathways: UV-B absorption by the chromophore and Fluorescence Resonant Energy Transfer (FRET) from tryptophan and tyrosine residues.
Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
Elizabeth C
EC
Carroll
Marijke
M
Hospes
Carmen
C
Valladares
Klaas J
KJ
Hellingwerf
Delmar S
DS
Larsen
D001426:Bacterial Proteins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D020607:Halorhodospira halophila / Q000378:metabolism / Q000528:radiation effects; D007700:Kinetics; D020283:Photoreceptors, Microbial / Q000737:chemistry*; D011789:Quantum Theory; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; C050162:photoactive yellow protein, Bacteria; D014443:Tyrosine; D014364:Tryptophan