Genetic and molecular analysis of a purple sheath somaclonal mutant in japonica rice.
Natural and artificially induced mutants have provided valuable resources for plant genetic studies and crop improvement. In this study, we investigated the genetic and molecular basis of the purple sheath trait in a somaclonal mutant Z418, which was regenerated from a green sheath rice variety C418 through tissue culture. The purple sheath trait in Z418 was heritable and stable based on our 10 years of evaluation. Genetic analysis revealed that the purple sheath trait of the mutant was controlled by a single dominant gene. To map the gene, we scored 89 polymorphic SSRs markers in a F(2) population of 232 plants derived from a cross between Z418 and HX-3, an indica variety with green sheath trait. The gene was initially mapped to the short arm of chromosome 6 between two SSR markers, RPM5 and RM402, with a genetic distance of 1.1 and 10.3 cM, respectively. Thirty-one SSR and indel markers located within the target region were further used to fine-map the gene to a 153-kb interval between two SSR markers (RPM8 and RPM11). The OsC1 gene, which locates within the region and encodes a MYB family transcription factor, was chosen as the candidate gene controlling the purple sheath trait in Z418. Sequencing analysis revealed that OsC1 gene and its transcript in Z418 was 34 bp longer than that in C418. The possible mechanisms for the gene mutation, the developmental and tissue-specific expression of purple anthocyanin pigmentation in Z418, were finally discussed.
Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China. gao_dongying@yahoo.com.cn
Dongying
D
Gao
Bing
B
He
Yihong
Y
Zhou
Lihua
L
Sun
D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000378:metabolism; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D005799:Genes, Dominant; D017343:Genes, Plant / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D010802:Phylogeny; D010858:Pigmentation / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D046509:Tissue Culture Techniques
D000872:Anthocyanins
Plant Mol Biol. 2003 May;52(1):69-79
12825690
Nature. 2003 Jan 9;421(6919):163-7
12520302
Mol Genet Genomics. 2001 Mar;265(1):32-42
11370870
Plant J. 2006 Jan;45(1):46-57
16367953
Theor Appl Genet. 2004 Dec;110(1):157-66
15678329
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7783-8
8755553
Genetics. 2004 Oct;168(2):997-1007
15514070
Theor Appl Genet. 1985 Nov;71(1):133-5
24247351
Nature. 2005 Aug 11;436(7052):793-800
16100779
Theor Appl Genet. 1987 May;74(1):104-12
24241465
Genetics. 1991 May;128(1):163-73
1648001
Plant J. 2001 Sep;27(5):467-78
11576430
Nature. 2004 Sep 30;431(7008):569-73
15457261
Theor Appl Genet. 1981 Oct;60(4):197-214
24276737
Bioinformatics. 2007 Nov 1;23(21):2947-8
17846036
Ann Bot. 2007 Nov;100(5):959-66
17704538
Plant Cell. 2005 Mar;17(3):903-14
15722466
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8244-8
8367489
DNA Res. 2002 Dec 31;9(6):199-207
12597276
Theor Appl Genet. 1991 Feb;81(2):157-61
24221197
Nucleic Acids Res. 2007 Jan;35(Database issue):D883-7
17145706
Plant Cell Rep. 2004 Dec;23(7):492-6
15372197
Genome Res. 2001 Aug;11(8):1441-52
11483586
Genome. 2009 Mar;52(3):268-74
19234555
Theor Appl Genet. 2004 Jun;109(1):200-9
15071728
Mol Genet Genomics. 2005 Apr;273(2):150-7
15803319
Plant Mol Biol. 2003 Sep;53(1-2):75-86
14756308
Transgenic Res. 1996 Mar;5(2):97-103
8866891
Plant J. 2006 Sep;47(6):969-76
16961734
Plant J. 2007 Jan;49(1):91-102
17163879
Nature. 2005 Mar 24;434(7032):505-9
15785770
Plant Cell. 1997 Sep;9(9):1547-1557
12237395
Plant Cell. 2006 Feb;18(2):283-94
16399804
Theor Appl Genet. 1990 Nov;80(5):673-9
24221075
Genetics. 2010 Nov;186(3):843-55
20823340
Genes Dev. 1992 Nov;6(11):2152-64
1427078
Mol Biol Evol. 2007 Aug;24(8):1596-9
17488738
Theor Appl Genet. 1998 Mar;96(3-4):421-5
24710881
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15007-12
10611328
Yi Chuan Xue Bao. 2002 Feb;29(2):138-43
11901997
Plant Cell. 2001 Sep;13(9):2099-114
11549766
Plant Cell Physiol. 2006 Apr;47(4):457-70
16446312
Genomics. 1987 Oct;1(2):174-81
3692487