Systemic distribution, nuclear entry and cytotoxicity of amorphous nanosilica following topical application.
Currently, nanomaterials (NMs) with particle sizes below 100 nm have been successfully employed in various industrial applications in medicine, cosmetics and foods. On the other hand, NMs can also be problematic in terms of eliciting a toxicological effect by their small size. However, biological and/or cellular responses to NMs are often inconsistent and even contradictory. In addition, relationships among NMs physicochemical properties, absorbency, localization and biological responses are not yet well understood. In order to open new frontiers in medical, cosmetics and foods fields by the safer NMs, it is necessary to collect the information of the detailed properties of NMs and then, build the prediction system of NMs safety. The present study was designed to examine the skin penetration, cellular localization, and cytotoxic effects of the well-dispersed amorphous silica particles of diameters ranging from 70 nm to 1000 nm. Our results suggested that the well-dispersed amorphous nanosilica of particle size 70 nm (nSP70) penetrated the skin barrier and caused systemic exposure in mouse, and induced mutagenic activity in vitro. Our information indicated that further studies of relation between physicochemical properties and biological responses are needed for the development and the safer form of NMs.
Department of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Hiromi
H
Nabeshi
Tomoaki
T
Yoshikawa
Keigo
K
Matsuyama
Yasutaro
Y
Nakazato
Kazuhiko
K
Matsuo
Akihiro
A
Arimori
Masaaki
M
Isobe
Saeko
S
Tochigi
Sayuri
S
Kondoh
Toshiro
T
Hirai
Takanori
T
Akase
Takuya
T
Yamashita
Kohei
K
Yamashita
Tokuyuki
T
Yoshida
Kazuya
K
Nagano
Yasuhiro
Y
Abe
Yasuo
Y
Yoshioka
Haruhiko
H
Kamada
Takayoshi
T
Imazawa
Norio
N
Itoh
Shinsaku
S
Nakagawa
Tadanori
T
Mayumi
Shin-Ichi
S
Tsunoda
Yasuo
Y
Tsutsumi
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests; D049329:Nanostructures / Q000009:adverse effects* / Q000737:chemistry*; D012822:Silicon Dioxide / Q000009:adverse effects* / Q000737:chemistry / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
D012822:Silicon Dioxide