Methanogen community structure in the rumens of farmed sheep, cattle and red deer fed different diets.
Development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on knowledge of the methanogens present. We investigated the composition of archaeal communities in the rumens of farmed sheep (Ovis aries), cattle (Bos taurus) and red deer (Cervus elaphus) using denaturing gradient gel electrophoresis (DGGE) to generate fingerprints of archaeal 16S rRNA genes. The total archaeal communities were relatively constant across species and diets, and were less variable and less diverse than bacterial communities. There were diet- and ruminant-species-based differences in archaeal community structure, but the same dominant archaea were present in all rumens. These were members of three coherent clades: species related to Methanobrevibacter ruminantium and Methanobrevibacter olleyae; species related to Methanobrevibacter gottschalkii, Methanobrevibacter thaueri and Methanobrevibacter millerae; and species of the genus Methanosphaera. Members of an archaeal group of unknown physiology, designated rumen cluster C (RCC), were also present. RCC-specific DGGE, clone library analysis and quantitative real-time PCR showed that their 16S rRNA gene sequences were very diverse and made up an average of 26.5% of the total archaea. RCC sequences were not readily detected in the DGGE patterns of total archaeal 16S rRNA genes because no single sequence type was abundant enough to form dominant bands.
AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand.
Jeyamalar
J
Jeyanathan
Marek
M
Kirs
Ron S
RS
Ronimus
Simone O
SO
Hoskin
Peter H
PH
Janssen
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics; D002417:Cattle / Q000382:microbiology; D019641:DNA, Archaeal / Q000235:genetics; D003670:Deer / Q000382:microbiology; D058645:Denaturing Gradient Gel Electrophoresis; D004032:Diet; D015723:Gene Library; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D008697:Methane; D017013:Methanobacteriaceae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D034561:Sheep, Domestic / Q000382:microbiology
D019641:DNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008697:Methane