Dark-phase light contamination disrupts circadian rhythms in plasma measures of endocrine physiology and metabolism in rats.
Dark-phase light contamination can significantly disrupt chronobiologic rhythms, thereby potentially altering the endocrine physiology and metabolism of experimental animals and influencing the outcome of scientific investigations. We sought to determine whether exposure to low-level light contamination during the dark phase influenced the normally entrained circadian rhythms of various substances in plasma. Male Sprague-Dawley rats (n = 6 per group) were housed in photobiologic light-exposure chambers configured to create 1) a 12:12-h light:dark cycle without dark-phase light contamination (control condition; 123 μW/cm(2), lights on at 0600), 2) experimental exposure to a low level of light during the 12-h dark phase (with 0.02, 0.05, 0.06, or 0.08 μW/cm(2) light at night), or 3) constant bright light (123 μW/cm(2)). Dietary and water intakes were recorded daily. After 2 wk, rats underwent 6 low-volume blood draws at 4-h intervals (beginning at 0400) during both the light and dark phases. Circadian rhythms in dietary and water intake and levels of plasma total fatty acids and lipid fractions remained entrained during exposure to either control conditions or low-intensity light during the dark phase. However, these patterns were disrupted in rats exposed to constant bright light. Circadian patterns of plasma melatonin, glucose, lactic acid, and corticosterone were maintained in all rats except those exposed to constant bright light or the highest level of light during the dark phase. Therefore even minimal light contamination during the dark phase can disrupt normal circadian rhythms of endocrine metabolism and physiology and may alter the outcome of scientific investigations.
Department of Structural & Cellular Biology, Tulane University School of Medicine, Tulane, Louisiana, USA. rdauchy@tulane.edu
Robert T
RT
Dauchy
Erin M
EM
Dauchy
Robert P
RP
Tirrell
Cody R
CR
Hill
Leslie K
LK
Davidson
Michael W
MW
Greene
Paul C
PC
Tirrell
Jinghai
J
Wu
Leonard A
LA
Sauer
David E
DE
Blask
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002940:Circadian Rhythm / Q000528:radiation effects*; D003345:Corticosterone / Q000097:blood; D004703:Endocrine System / Q000528:radiation effects; D005227:Fatty Acids / Q000097:blood; D006799:Housing, Animal; D007756:Laboratory Animal Science; D019344:Lactic Acid / Q000097:blood; D008027:Light; D008297:Male; D008550:Melatonin / Q000097:blood; D017440:Photoperiod; D051381:Rats / Q000378:metabolism / Q000502:physiology*; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D005227:Fatty Acids; D019344:Lactic Acid; D008550:Melatonin; D003345:Corticosterone
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