Aluminum exposure alters behavioral parameters and increases acetylcholinesterase activity in zebrafish (Danio rerio) brain.
Aluminum is a metal that is known to impact fish species. The zebrafish has been used as an attractive model for toxicology and behavioral studies, being considered a model to study environmental exposures and human pathologies. In the present study, we have investigated the effect of aluminum exposure on brain acetylcholinesterase activity and behavioral parameters in zebrafish. In vivo exposure of zebrafish to 50 μg/L AlCl(3) for 96 h at pH 5.8 significantly increased (36%) acetylthiocholine hydrolysis in zebrafish brain. There were no changes in acetylcholinesterase (AChE) activity when fish were exposed to the same concentration of AlCl(3) at pH 6.8. In vitro concentrations of AlCl(3) varying from 50 to 250 μM increased AChE activity (28% to 33%, respectively). Moreover, we observed that animals exposed to AlCl(3) at pH 5.8 presented a significant decrease in locomotor activity, as evaluated by the number of line crossings (25%), distance traveled (14.1%), and maximum speed (24%) besides an increase in the absolute turn angle (12.7%). These results indicate that sublethal levels of aluminum might modify behavioral parameters and acetylcholinesterase activity in zebrafish brain.
Laboratory of Proteins and Peptides Biochemistry, Oswaldo Cruz Institute-FIOCRUZ, 21045-900, Rio de Janeiro, RJ, Brazil.
Mario Roberto
MR
Senger
Kelly Juliana
KJ
Seibt
Gabriele Cordenonzi
GC
Ghisleni
Renato Dutra
RD
Dias
Mauricio Reis
MR
Bogo
Carla Denise
CD
Bonan
D000110:Acetylcholinesterase / Q000378:metabolism*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002712:Chlorides / Q000633:toxicity*; D004781:Environmental Exposure / Q000009:adverse effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D057075:Enzyme Assays; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D015027:Zebrafish
D017607:Aluminum Compounds; D002712:Chlorides; D004785:Environmental Pollutants; D000077410:Aluminum Chloride; D000110:Acetylcholinesterase