Oligonucleotide analogs with peptide internucleotide linkages.
Oligonucleotide analogs containing one or a few glycine, L-, and D-alanine or L-and D-phenylalanine residues instead of phosphodiesterinternucleotide linkages were synthesized. The stability of the duplexes formed by modified oligonucleotides and their wildtype complements was studied. Oligonucleotides with D-alanine residues in internucleotide linkages form duplexes more stable than native ones (ΔT(m) +0.2 °C per modification), whereas other modifications destabilize the duplexes.
Engelhardt Institute of Molecular Biology, Moscow, Russian Federation.
Anna
A
Varizhuk
Svetlana
S
Kochetkova
Natalia
N
Kolganova
Edward
E
Timofeev
Vladimir
V
Florentiev
D000409:Alanine / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*; D010649:Phenylalanine / Q000737:chemistry
D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D010649:Phenylalanine; D000409:Alanine; D005998:Glycine