Genotyping mdx, mdx3cv, and mdx4cv mice by primer competition polymerase chain reaction.
mdx, mdx3cv, and mdx4cv mice are among the most commonly used models for the study of Duchenne muscular dystrophy. Their disease is caused by point mutations in the dystrophin gene. Despite widespread use of these models, genotyping has not always been straightforward. Current methods require multiple polymerase chain reactions (PCRs), post-PCR manipulations, and/or special equipment/reagents. Herein we report a simple, robust PCR genotyping method based on primer competition. This approach could also be applied in genotyping other point-mutation models.
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA.
Jin-Hong
JH
Shin
Chady H
CH
Hakim
Keqing
K
Zhang
Dongsheng
D
Duan
D000818:Animals; D017931:DNA Primers / Q000235:genetics*; D004195:Disease Models, Animal; D016189:Dystrophin / Q000235:genetics*; D005838:Genotype; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics* / Q000503:physiopathology*; D017354:Point Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D016189:Dystrophin; C085339:apo-dystrophin 1
J Cell Biochem. 1991 Jul;46(3):255-9
1774226
Biotechniques. 2000 Jul;29(1):52, 54
10907076
Muscle Nerve. 1996 Dec;19(12):1549-53
8941268
Proc Natl Acad Sci U S A. 1989 Feb;86(4):1292-6
2919177
Muscle Nerve. 1992 Oct;15(10):1133-7
1357549
Neuromuscul Disord. 2002 May;12(4):366-70
12062254
Nat Genet. 1993 May;4(1):87-93
8099842
Muscle Nerve. 2010 Aug;42(2):268-70
20544945
Biotechniques. 1997 May;22(5):964-8
9149883
Science. 1989 Jun 30;244(4912):1578-80
2662404
Muscle Nerve. 2008 Jun;37(6):731-5
18506711
Hum Mol Genet. 1996 Aug;5(8):1149-53
8842734
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1189-92
6583703
Development. 1999 Oct;126(20):4441-53
10498680
Am J Vet Res. 1996 May;57(5):650-4
8723876
Hum Mutat. 1998;11(3):252-8
9521428
Muscle Nerve. 2009 May;39(5):603-8
19347923