Autoregulation of nodulation interferes with impacts of nitrogen fertilization levels on the leaf-associated bacterial community in soybeans.
The diversities leaf-associated bacteria on nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S rRNA gene. To analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitrogen (SN) (15 kg N ha(-1)) or heavy nitrogen (HN) (615 kg N ha(-1)) fertilization. Under SN fertilization, the relative abundance of Alphaproteobacteria was significantly higher in Nod(-) and Nod(++) soybeans (82% to 96%) than in Nod(+) soybeans (54%). The community structure of leaf-associated bacteria in Nod(+) soybeans was almost unaffected by the levels of nitrogen fertilization. However, differences were visible in Nod(-) and Nod(++) soybeans. HN fertilization drastically decreased the relative abundance of Alphaproteobacteria in Nod(-) and Nod(++) soybeans (46% to 76%) and, conversely, increased those of Gammaproteobacteria and Firmicutes in these mutant soybeans. In the Alphaproteobacteria, cluster analyses identified two operational taxonomic units (OTUs) (Aurantimonas sp. and Methylobacterium sp.) that were especially sensitive to nodulation phenotypes under SN fertilization and to nitrogen fertilization levels. Arbuscular mycorrhizal infection was not observed on the root tissues examined, presumably due to the rotation of paddy and upland fields. These results suggest that a subpopulation of leaf-associated bacteria in wild-type Nod(+) soybeans is controlled in similar ways through the systemic regulation of autoregulation of nodulation, which interferes with the impacts of N levels on the bacterial community of soybean leaves.
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
Seishi
S
Ikeda
Mizue
M
Anda
Shoko
S
Inaba
Shima
S
Eda
Shusei
S
Sato
Kazuhiro
K
Sasaki
Satoshi
S
Tabata
Hisayuki
H
Mitsui
Tadashi
T
Sato
Takuro
T
Shinano
Kiwamu
K
Minamisawa
D020561:Alphaproteobacteria / Q000254:growth & development / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism; D020563:Gammaproteobacteria / Q000254:growth & development / Q000378:metabolism; D020580:Methylobacterium / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D055170:Plant Root Nodulation / Q000235:genetics / Q000502:physiology*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D013559:Symbiosis / Q000235:genetics / Q000502:physiology
D009584:Nitrogen
Appl Environ Microbiol. 2006 Oct;72(10):6773-9
17021230
Appl Environ Microbiol. 2005 Mar;71(3):1501-6
15746353
Nature. 2002 Nov 28;420(6914):426-9
12442172
Appl Environ Microbiol. 2008 Sep;74(18):5704-9
18658280
Planta. 2000 Jun;211(1):98-104
10923709
BMC Microbiol. 2007 Nov 28;7:106
18045498
Genome Res. 1998 Mar;8(3):186-94
9521922
Science. 2003 Jan 3;299(5603):109-12
12411574
Microb Ecol. 1997 Sep;34(2):118-24
9230099
Appl Environ Microbiol. 2002 Jan;68(1):326-34
11772642
ISME J. 2010 Jun;4(6):752-63
20147983
Plant Cell Physiol. 2010 Sep;51(9):1381-97
20660226
J Appl Microbiol. 2005;99(5):1181-90
16238749
Microb Ecol. 2009 Nov;58(4):703-14
19662454
Mol Plant Microbe Interact. 2007 Aug;20(8):994-1003
17722702
New Phytol. 2010 Mar;185(4):1074-86
20100211
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
17586664
ISME J. 2010 Jan;4(1):17-27
19710709
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4162-6
16593577
Microbes Environ. 2009;24(3):253-8
21566381
Trends Microbiol. 2008 Oct;16(10):463-71
18789693
ISME J. 2010 Mar;4(3):315-26
19924157
FEMS Microbiol Ecol. 2004 Feb 1;47(2):161-77
19712332
Appl Environ Microbiol. 2005 Dec;71(12):8228-35
16332807
Evolution. 1985 Jul;39(4):783-791
28561359
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1115-1122
12892136
Genome Res. 1998 Mar;8(3):175-85
9521921
Appl Environ Microbiol. 2007 Feb;73(3):913-21
17142371
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417
Appl Environ Microbiol. 2006 Sep;72(9):5734-41
16957188
Curr Opin Plant Biol. 2006 Oct;9(5):496-502
16877028
Plant Cell Physiol. 2009 Jan;50(1):67-77
19074184
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2158-63
18768622
Comput Appl Biosci. 1996 Aug;12(4):357-8
8902363
Plant Cell Physiol. 2010 Sep;51(9):1398-410
20685969
Nature. 2003 Oct 9;425(6958):585-92
14534578