Characterization of Burkholderia rhizoxinica and B. endofungorum isolated from clinical specimens.
Eight isolates submitted to CDC from 1989 to 2006 from clinical specimens were initially identified as members of the genus Burkholderia based on preliminary cellular fatty acid analysis and/or 16S rRNA gene sequencing. With the recent descriptions of the new species B. rhizoxinica and B. endofungorum, which are considered endosymbiotic bacteria in Rhizopus microsporus fungi, we now identify seven of these clinical isolates as B. rhizoxinica and one as B. endofungorum based on biochemical testing, 16s rRNA, and DNA-DNA hybridization results. We also further characterize these isolates by assessing toxin production and/or by multiple locus sequence typing.
Bacterial Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. JGee1@cdc.gov
Jay E
JE
Gee
Mindy B
MB
Glass
Gerald
G
Lackner
Leta O
LO
Helsel
Maryam
M
Daneshvar
Dannie G
DG
Hollis
Jean
J
Jordan
Roger
R
Morey
Arnold
A
Steigerwalt
Christian
C
Hertweck
D001427:Bacterial Toxins / Q000032:analysis; D019117:Burkholderia / Q000145:classification / Q000302:isolation & purification*; D002487:Centers for Disease Control and Prevention, U.S.; D005227:Fatty Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D013559:Symbiosis; D014481:United States
D001427:Bacterial Toxins; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
Microbiol Immunol. 1992;36(12):1251-75
1283774
J Am Chem Soc. 2006 Sep 6;128(35):11529-36
16939276
Appl Environ Microbiol. 2009 May;75(9):2982-6
19286793
J Clin Microbiol. 2003 Oct;41(10):4647-54
14532197
Mycoses. 2008 May;51(3):266-9
18399908
Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2583-2590
17978222
J Clin Microbiol. 2006 Dec;44(12):4601-4
17050819
Int J Syst Evol Microbiol. 2006 Sep;56(Pt 9):2171-2176
16957116
Science. 2001 Dec 14;294(5550):2310-4
11743192
Chembiochem. 2007 Jan 2;8(1):41-5
17154220
J Antibiot (Tokyo). 1984 Apr;37(4):354-62
6547134
Food Chem Toxicol. 1984 Apr;22(4):275-81
6539275
Comput Appl Biosci. 1996 Aug;12(4):357-8
8902363
Clin Microbiol Rev. 2000 Apr;13(2):236-301
10756000
J Infect Dis. 2008 Oct 1;198(7):1083-90
18694335
J Clin Microbiol. 1982 Jun;15(6):1133-40
7107843
Environ Microbiol. 2003 Sep;5(9):719-29
12919407
Mol Biol Evol. 2006 Feb;23(2):254-67
16221896
Curr Biol. 2007 May 1;17(9):773-7
17412585
Appl Environ Microbiol. 2007 Feb;73(3):793-7
17122400
Bioinformatics. 2001 Aug;17(8):754-5
11524383
Brief Bioinform. 2004 Jun;5(2):150-63
15260895
Nature. 2005 Oct 6;437(7060):884-8
16208371
Trends Microbiol. 2009 Dec;17(12):570-6
19800796