Facile one-step catalytic grafting of N-hydroxysuccinimidyl-ester-functionalized methallylsilane onto silica for enzyme immobilization.
A one-step grafting method of NHS-ester group is described for the immobilization of enzymes onto a silica surface at room temperature. Pure NHS-ester functionalized methallylsilane was prepared as a grafting reagent and showed remarkable grafting efficiency with acid catalysts at room temperature. As a result, mesoporous silica balls bearing a covalently immobilized enzyme could be obtained with a high loading rate compared to conventional multistep grafting methods. This one-step procedure offers the advantages of precise measurement of the high loading efficiency of the NHS-ester group, and the exclusive formation of only the NHS-ester group, which reduces the presence of unreacted functionalities.
Department of Chemistry and Center for Bioactive Molecular Hybrid (CBMH), Yonsei University, Seongsanno 262, Seodaemun-gu, Seoul 120-749, Korea.
Un-Young
UY
Jung
Jung-Woo
JW
Park
Eun-Hee
EH
Han
Sin-Gun
SG
Kang
Sera
S
Lee
Chul-Ho
CH
Jun
D001234:Aspergillus niger / Q000201:enzymology*; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry*
D004800:Enzymes, Immobilized; D004952:Esters; D013388:Succinimides; D012822:Silicon Dioxide; D005949:Glucose Oxidase