The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting.
Protein ubiquitylation regulates a broad variety of biological processes in all eukaryotes. Recent work identified a novel class of cullin-containing ubiquitin ligases (E3s) composed of CUL4, DDB1, and one WD40 protein, believed to act as a substrate receptor. Strikingly, CUL4-based E3 ligases (CRL4s) have important functions at the chromatin level, including responses to DNA damage in metazoans and plants and, in fission yeast, in heterochromatin silencing. Among putative CRL4 receptors we identified MULTICOPY SUPPRESSOR OF IRA1 (MSI1), which belongs to an evolutionary conserved protein family. MSI1-like proteins contribute to different protein complexes, including the epigenetic regulatory Polycomb repressive complex 2 (PRC2). Here, we provide evidence that Arabidopsis MSI1 physically interacts with DDB1A and is part of a multimeric protein complex including CUL4. CUL4 and DDB1 loss-of-function lead to embryo lethality. Interestingly, as in fis class mutants, cul4 mutants exhibit autonomous endosperm initiation and loss of parental imprinting of MEDEA, a target gene of the Arabidopsis PRC2 complex. In addition, after pollination both MEDEA transcript and protein accumulate in a cul4 mutant background. Overall, our work provides the first evidence of a physical and functional link between a CRL4 E3 ligase and a PRC2 complex, thus indicating a novel role of ubiquitylation in the repression of gene expression.
Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, Conventionné avec l'Université de Strasbourg, Strasbourg, France.
Eva
E
Dumbliauskas
Esther
E
Lechner
Miłosława
M
Jaciubek
Alexandre
A
Berr
Maghsoud
M
Pazhouhandeh
Malek
M
Alioua
Valerie
V
Cognat
Vladimir
V
Brukhin
Csaba
C
Koncz
Ueli
U
Grossniklaus
Jean
J
Molinier
Pascal
P
Genschik
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D018392:Genomic Imprinting / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D054875:Ubiquitination / Q000502:physiology
D029681:Arabidopsis Proteins; C514806:CULLIN4 protein, Arabidopsis; D044842:Cullin Proteins; C517426:DDB1a protein, Arabidopsis; D004268:DNA-Binding Proteins; C111712:MEA protein, Arabidopsis; C475821:MSI1 protein, Arabidopsis; D046912:Multiprotein Complexes; D012097:Repressor Proteins
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