Ear keloids as a primary candidate for the application of mitomycin C after shave excision: in vivo and in vitro study.
BACKGROUND
Although many methods have been developed to treat ear keloids, new therapeutic options are still needed.


OBJECTIVE
To determine the effects of topical mitomycin C (MC) on shave-removed wounds and fibroblasts of ear keloids.


METHODS
Fourteen ear keloids in 12 patients were shaved, and MC (1 mg/mL) was applied to the resected bed for 5 minutes. The application was repeated 3 weeks later. All patients were assessed 2, 4, and 6 months after the procedure to evaluate the cosmetic results, recurrence, and postsurgical complications. An in vitro study to determine the effects of MC on fibroblasts of the excised keloids was conducted using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the measurement of total cell counts, and immunoassay of DNA synthesis.


RESULTS
Only one recurrence occurred (on the ear helix), and the patients were satisfied with the cosmetic outcomes. The results of the MTT assay, total cell counts, and DNA synthesis immunoassay confirmed the suppressive effects of MC on the keloid fibroblasts.


CONCLUSIONS
The application of topical MC to the resected bed of shave-removed ear keloids was successful in preventing recurrences and providing an acceptable cosmetic outcome.
Department of Dermatology, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
Seong Geun
SG
Chi
Jun Young
JY
Kim
Weon Ju
WJ
Lee
Seok-Jong
SJ
Lee
Do Won
DW
Kim
Mi Yeung
MY
Sohn
Gun Wook
GW
Kim
Moon Bum
MB
Kim
Byung Soo
BS
Kim
D000293:Adolescent; D000328:Adult; D046288:Body Piercing / Q000009:adverse effects; D018929:Cell Culture Techniques; D004431:Ear, External; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007627:Keloid / Q000188:drug therapy* / Q000209:etiology / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology / Q000627:therapeutic use*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D055815:Young Adult
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin