Click assembly of magnetic nanovectors for gene delivery.
Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe(3)O(4) nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.
Department of Chemistry, University of Louisville, Louisville, KY 40292, USA.
Souvik
S
Biswas
Laura E
LE
Gordon
Geoffrey J
GJ
Clark
Michael H
MH
Nantz
D045744:Cell Line, Tumor; D002470:Cell Survival; D057930:Click Chemistry / Q000379:methods*; D004247:DNA / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055672:Static Electricity; D014162:Transfection / Q000379:methods*
D008055:Lipids; D004247:DNA
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