Sustained torpidity following multi-dose administration of 3-iodothyronamine in mice.
Despite significant medical benefits as in space exploration or emergency care, prolonged torpidity of non-hibernator mammals remains unexplored to date. Here, we report that male Institute of Cancer Research mice could sustain two separate 2-day torpor bouts and maintain body temperature of 28-33°C following repeated treatments of 3-iodothyronamine (T(1) AM), a natural derivative of thyroid hormone. A 1-day interbout arousal period, adopted to mimic the behavior of true hibernators, seemed critical for the subjects to restore physiological homeostasis. Molecular studies of neuron-specific enolase, S100 calcium binding protein B and heat shock protein 72 suggested that the brain maintains functional and cytoprotective activities during sustained torpidity. Together, the results of this study propose a practical protocol using a torpor-arousal cycle that can be applied to the extreme medical situations.
Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Wonju, Gangwon-Do, Republic of Korea.
Hyunwoo
H
Ju
Hyekyoung
H
So
Kyungbong
K
Ha
Kyoungsook
K
Park
Ju-Woon
JW
Lee
Chan-Moon
CM
Chung
Inho
I
Choi
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006605:Hibernation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009420:Nervous System / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D013970:Thyronines / Q000008:administration & dosage* / Q000494:pharmacology*; D013997:Time Factors
C487948:3-iodothyronamine; D013970:Thyronines