Autotaxin is required for the cranial neural tube closure and establishment of the midbrain-hindbrain boundary during mouse development.
Autotaxin (ATX) is a lysophospholipid-generating exoenzyme expressed in embryonic and adult neural tissues. We previously showed that ATX is expressed in the neural organizing centers, anterior head process, and midbrain-hindbrain boundary (MHB). To elucidate the role of ATX during neural development, here we examined the neural phenotypes of ATX-deficient mice. Expression analysis of neural marker genes revealed that lateral expansion of the rostral forebrain is reduced and establishment of the MHB is compromised as early as the late headfold stage in ATX mutant embryos. Moreover, ATX mutant embryos fail to complete cranial neural tube closure. These results indicate that ATX is essential for cranial neurulation and MHB establishment.
Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Seiichi
S
Koike
Yoshifumi
Y
Yutoh
Kazuko
K
Keino-Masu
Sumihare
S
Noji
Masayuki
M
Masu
Hideyo
H
Ohuchi
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004622:Embryo, Mammalian / Q000033:anatomy & histology / Q000502:physiology; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000033:anatomy & histology / Q000196:embryology*; D051379:Mice; D018345:Mice, Knockout; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D054261:Neurulation / Q000502:physiology*; D051857:Otx Transcription Factors / Q000235:genetics / Q000378:metabolism; D043264:Phosphodiesterase I / Q000235:genetics / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism*; D012249:Rhombencephalon / Q000033:anatomy & histology / Q000196:embryology*; D000097607:Homeobox Protein SIX3; D000099189:Lysophospholipase D
D015415:Biomarkers; D005136:Eye Proteins; D051524:Fibroblast Growth Factor 8; D018398:Homeodomain Proteins; D009097:Multienzyme Complexes; D009419:Nerve Tissue Proteins; D051857:Otx Transcription Factors; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000097607:Homeobox Protein SIX3; D000099189:Lysophospholipase D; C496457:Fgf8 protein, mouse; C497660:Otx2 protein, mouse