Establishment of A431 cell membrane chromatography-RPLC method for screening target components from Radix Caulophylli.
We describe here an analytical method of A431 cell membrane chromatography (A431/CMC) (CMC, cell membrane chromatography) combined with RPLC for recognition, separation, and identification of target components from traditional Chinese medicines (TCMs) Radix Caulophylli. The A431 cells with high expressed epidermal growth factor receptor (EGFR) were used to prepare the stationary phase in the CMC model. Retention fractions on the A431-CMC model were collected using an automated fraction collection and injection module (FC/I). Each fraction was analyzed by RPLC under the optimized conditions. Gefitinib and erlotinib were used as standard compounds to investigate the suitability and reliability of the A431 cell membrane chromatography-RPLC method prior to screening target component from Radix Caulophylli total alkaloids. The results indicated that caulophine and taspine were the target component acting on the epidermal growth factor receptor. This method could be an efficient way in drug discovery using natural medicinal herbs as a source of novel compounds.
School of Medicine, Xi'an Jiaotong University, Shaanxi, PR China.
Xiaofang
X
Hou
Sicen
S
Wang
Jingjing
J
Hou
Langchong
L
He
D000470:Alkaloids / Q000737:chemistry; D028145:Caulophyllum / Q000737:chemistry*; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D066246:ErbB Receptors / Q000737:chemistry; D006801:Humans; D011485:Protein Binding
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D066246:ErbB Receptors