Beta-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana.
Most plant glycoproteins contain substantial amounts of paucimannosidic N-glycans instead of their direct biosynthetic precursors, complex N-glycans with terminal N-acetylglucosamine residues. We now demonstrate that two β-N-acetylhexosaminidases (HEXO1 and HEXO3) residing in different subcellular compartments jointly account for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. Total N-glycan analysis of hexo knock-out plants revealed that HEXO1 and HEXO3 contribute equally to the production of paucimannosidic N-glycans in roots, whereas N-glycan processing in leaves depends more heavily on HEXO3 than on HEXO1. Because hexo1 hexo3 double mutants do not display any obvious phenotype even upon exposure to different forms of abiotic or biotic stress, it should be feasible to improve the quality of glycoprotein therapeutics produced in plants by down-regulation of endogenous β-N-acetylhexosaminidase activities.
Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, A-1190 Vienna, Austria.
Eva
E
Liebminger
Christiane
C
Veit
Martin
M
Pabst
Martine
M
Batoux
Cyril
C
Zipfel
Friedrich
F
Altmann
Lukas
L
Mach
Richard
R
Strasser
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D011134:Polysaccharides; D001619:beta-N-Acetylhexosaminidases
Proteomics. 2005 Jan;5(1):212-21
15593128
Plant J. 2004 Aug;39(3):440-9
15255872
Plant Cell. 2006 Feb;18(2):465-76
16377758
Anal Chem. 2007 Jul 1;79(13):5051-7
17539604
Plant Biotechnol J. 2004 Nov;2(6):517-23
17147624
Proteomics. 2008 Jul;8(14):2858-71
18655055
Plant Physiol. 2007 Sep;145(1):5-16
17644627
FEBS Lett. 2004 Mar 12;561(1-3):132-6
15013764
Trends Plant Sci. 1999 Nov;4(11):424-428
10529823
Traffic. 2009 Jan;10(1):101-15
18939950
Biosci Biotechnol Biochem. 2006 Apr;70(4):980-7
16636467
J Immunol. 2010 Dec 1;185(11):6876-82
21041724
Planta. 2001 Jan;212(2):294-304
11216851
Anal Biochem. 1984 Apr;138(1):141-3
6731838
J Biol Chem. 2009 Jul 31;284(31):20479-85
19478090
Glycoconj J. 1999 Dec;16(12):787-91
11229321
Plant Biotechnol J. 2008 May;6(4):392-402
18346095
J Biol Chem. 2010 Feb 12;285(7):4629-36
20007973
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22522-7
20007779
Plant Physiol. 1996 Jan;110(1):195-202
8587982
Eur J Biochem. 2001 Aug;268(16):4570-9
11502219
Glycobiology. 2006 Apr;16(4):349-57
16373330
J Biotechnol. 2010 Mar;146(1-2):54-65
20083147
Nat Biotechnol. 2006 Dec;24(12):1591-7
17128273
Plant Biotechnol J. 2009 Jan;7(1):33-48
18778316
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2899-904
11226338
Glycobiology. 2007 Mar;17(3):334-44
17179169
Plant J. 1997 Dec;12(6):1411-7
9450345
Glycobiology. 2001 Apr;11(4):261-74
11358875
J Biol Chem. 2010 May 21;285(21):15923-30
20305285
Plant Mol Biol. 1998 Sep;38(1-2):31-48
9738959
Cell. 2006 May 19;125(4):749-60
16713565
Plant Cell. 2009 Dec;21(12):3850-67
20023195
Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4692-7
10200324
Planta. 2006 Jun;224(1):222-7
16395581
J Biol Chem. 1995 Jul 21;270(29):17344-9
7615537
Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3718-23
10737809
EMBO J. 2009 Nov 4;28(21):3428-38
19763086
Genetics. 2002 Jul;161(3):1279-91
12136030
Proc Natl Acad Sci U S A. 2008 Apr 15;105(15):5933-8
18408158
Eur J Biochem. 1991 Jul 1;199(1):169-79
2065672