Laboratory-evolved mutants of an exogenous global regulator, IrrE from Deinococcus radiodurans, enhance stress tolerances of Escherichia coli.
BACKGROUND
The tolerance of cells toward different stresses is very important for industrial strains of microbes, but difficult to improve by the manipulation of single genes. Traditional methods for enhancing cellular tolerances are inefficient and time-consuming. Recently, approaches employing global transcriptional or translational engineering methods have been increasingly explored. We found that an exogenous global regulator, irrE from an extremely radiation-resistant bacterium, Deinococcus radiodurans, has the potential to act as a global regulator in Escherichia coli, and that laboratory-evolution might be applied to alter this regulator to elicit different phenotypes for E. coli.


RESULTS
To extend the methodology for strain improvement and to obtain higher tolerances toward different stresses, we here describe an approach of engineering irrE gene in E. coli. An irrE library was constructed by randomly mutating the gene, and this library was then selected for tolerance to ethanol, butanol and acetate stresses. Several mutants showing significant tolerances were obtained and characterized. The tolerances of E. coli cells containing these mutants were enhanced 2 to 50-fold, based on cell growth tests using different concentrations of alcohols or acetate, and enhanced 10 to 100-fold based on ethanol or butanol shock experiments. Intracellular reactive oxygen species (ROS) assays showed that intracellular ROS levels were sharply reduced for cells containing the irrE mutants. Sequence analysis of the mutants revealed that the mutations distribute cross all three domains of the protein.


CONCLUSIONS
To our knowledge, this is the first time that an exogenous global regulator has been artificially evolved to suit its new host. The successes suggest the possibility of improving tolerances of industrial strains by introducing and engineering exogenous global regulators, such as those from extremophiles. This new approach can be applied alone or in combination with other global methods, such as global transcriptional machinery engineering (gTME) for strain improvements.
Department of Chemical Engineering, Tsinghua University, Beijing, China.
Tingjian
T
Chen
Jianqing
J
Wang
Rong
R
Yang
Jicong
J
Li
Min
M
Lin
Zhanglin
Z
Lin
D034301:Deinococcus / Q000235:genetics* / Q000502:physiology*; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D015723:Gene Library; D007218:Industrial Microbiology / Q000379:methods*; D009154:Mutation; D017382:Reactive Oxygen Species / Q000032:analysis; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*
D017382:Reactive Oxygen Species
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