Potential molecular targets in chemopreventative action of celecoxib: a proteomics analysis of J774.A1 macrophage-like cell line.
The overexpression of cyclooxygenase-2 (COX-2) enzyme has been strongly contributed to tumorigenesis. The efficacy of celecoxib as a selective COX-2 inhibitor has been shown in many studies, but the underlying mechanism as a chemopreventative agent has not yet been well known. For better understanding the chemopreventative molecular mechanisms, we used a comparative proteomics analysis of lipopolysaccharide (LPS) treated and untreated J774.A1 macrophage-like cell lines before and after treatment with celecoxib. Our findings define the contribution of several interesting proteins, including ferritin heavy chain, glyoxalase-1, cofilin, vimentin, and galectin-1, which could extend our understanding of the chemopreventative effects of celecoxib and provide new valuable tools for further anticancer research.
Toxicology and Pharmacology Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Iran.
Faegheh
F
Rezaie
Mona
M
Salimi
Mohammad Hossein
MH
Ghahremani
Behrouz
B
Vaziri
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D000068579:Celecoxib; D045744:Cell Line, Tumor; D018890:Chemoprevention; D052246:Cyclooxygenase 2 Inhibitors / Q000378:metabolism / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D009369:Neoplasms / Q000503:physiopathology; D040901:Proteomics; D011720:Pyrazoles / Q000378:metabolism / Q000494:pharmacology; D013449:Sulfonamides / Q000378:metabolism / Q000494:pharmacology
D000970:Antineoplastic Agents; D052246:Cyclooxygenase 2 Inhibitors; D008070:Lipopolysaccharides; D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib