[Anti-HIV activities of extracts from Chinese medicinal fromula Liangcha].
OBJECTIVE
To screen and evaluate the anti-HIV activities of extracts from Chinese medicinal formula Liangcha.


METHODS
The anti-HIV-1 activities of extracts from Chinese medicinal formula Liangcha were evaluated by cytotoxicity assay,syncytium reduction assay, protection for HIV-1 induced lytic assay, and ELISA assay for HIV-1 p24 antigen expression. The primary mechanisms were investigated by fusion inhibition assay, inhibition of viral replication in HIV-1 chronically infected H9 cell and inhibition assay of HIV-1 RT activity.


RESULTS
The extracts from Chinese medicinal formula Liangcha exhibited potent and broad-spectrum anti-HIV-1 activity on different HIV-1 strains with EC50 range 12.74 -116.87 microg/mL, but weakly inhibited HIV-2 replication. Meantime, it was not significantly cytotoxic in several T lymphocytes cell lines with CC50 range 564.79 - 1699.22 microg/mL. The activity of recombinant HIV-1 reverse transcriptase was inhibited by extracts from Chinese medicinal formula Liangcha, inhibition rate more than 50% at the concentration of 5.3 microg/mL. It also weakly inhibited the cell-to-cell fusion in co-culture with EC50 of 101.94 microg/mL.


CONCLUSIONS
The extracts from Chinese medicinal formula Liangcha exhibited potent and broad-spectrum anti-HIV-1 activity on different HIV-1 strains in vitro. Its anti-HIV-1 mechanism might be inhibiting HIV-1 reverse transcriptase and cell entry.
Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, Kunming 650223, China.
Wu-Qing
WQ
Liu
Lei-Ke
LK
Li
Rui-Rui
RR
Wang
Liu-Meng
LM
Yang
Tao
T
Peng
Yong-Tang
YT
Zheng
D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry; D035161:Bombacaceae / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D024882:Drug Resistance, Viral; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry; D014779:Virus Replication / Q000187:drug effects*
D019380:Anti-HIV Agents; D010936:Plant Extracts; D054303:HIV Reverse Transcriptase