Microwave-assisted extraction and in situ clean-up for the determination of fluoroquinolone antibiotics in chicken breast muscle by LC-MS/MS.
A new method was developed for the determination of six fluoroquinolone antibiotics including fleroxacin, levofloxacin, ciprofloxacin, lomfloxacin, enrofloxacin, and sparfloxacin in chicken breast muscle, in which the extraction and clean-up were performed in one step by microwave irradiation. The mixture of ACN containing 0.3% v/v phosphoric acid/water pH 3 (70:30, v/v) was used as the extraction solution and hexane was used as the clean-up solution. The extract was analyzed by liquid chromatography-tandem mass spectrometry system. The RSDs of intra- and inter-day obtained are in the range of 1.0-10.4 and 3.8-13.6%, respectively. In the three fortified levels of chicken breast muscle (20, 100, and 500 ng/g), the recoveries of fluoroquinolone antibiotics ranging from 66.0 to 97.2% are obtained. The LODs are in the range of 2.7-6.7 ng/g. This method simplifies the process of the sample preparation and reduces the operation errors.
College of Chemistry, Jilin University, Changchun, P. R. China.
Haoyan
H
Xu
Ligang
L
Chen
Lei
L
Sun
Xin
X
Sun
Xiaobo
X
Du
Jian
J
Wang
Tianyi
T
Wang
Qinglei
Q
Zeng
Hui
H
Wang
Yang
Y
Xu
Xiaopan
X
Zhang
Lan
L
Ding
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D024841:Fluoroquinolones / Q000032:analysis* / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D008872:Microwaves; D018482:Muscle, Skeletal / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones