Multimodal CARS microscopy of structured carbohydrate biopolymers.
We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(-1)-3400 cm(-1)), together with the commonly-measured C-H stretch (2750 cm(-1)-2970 cm(-1)) and SHG provide potentially important structural information and contrast in these materials.
Aaron D
AD
Slepkov
Andrew
A
Ridsdale
Adrian F
AF
Pegoraro
Douglas J
DJ
Moffatt
Albert
A
Stolow
Carbohydr Res. 2004 Feb 25;339(3):569-78
15013393
Opt Express. 2009 Feb 16;17(4):2984-96
19219203
Opt Express. 2008 Nov 24;16(24):19396-409
19030027
J Struct Biol. 2010 Jul;171(1):88-94
20206272
Opt Lett. 2001 Sep 1;26(17):1341-3
18049602
Annu Rev Anal Chem (Palo Alto Calif). 2008;1:883-909
20636101
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14658-63
17804796
J Phys Chem B. 2010 Aug 12;114(31):10200-8
20684644
Carbohydr Res. 2001 Aug 3;334(1):27-37
11470248
J Microsc. 2008 Apr;230(Pt 1):70-5
18387041
Virology. 2009 Nov 10;394(1):130-42
19747705
Opt Lett. 2003 Nov 15;28(22):2207-9
14649943
Science. 2007 Feb 9;315(5813):804-7
17289988
Angew Chem Int Ed Engl. 2005 May 30;44(22):3358-93
15861454
Opt Lett. 2006 Aug 15;31(16):2495-7
16880867
Can J Physiol Pharmacol. 1991 Jan;69(1):60-78
2036603
J Lipid Res. 2010 Jul;51(7):1729-37
20208058
Int J Pharm. 2007 Feb 1;329(1-2):1-11
17137734
J Struct Biol. 2008 Oct;164(1):140-5
18664383
Biophys J. 2005 Jul;89(1):581-91
15834003
Carbohydr Res. 2005 Nov 21;340(16):2563-8
16168973
Biophys J. 2006 Jul 15;91(2):728-35
16632501
J Exp Bot. 2010;61(2):587-95
20007198
Science. 2008 Dec 19;322(5909):1857-61
19095943
Opt Express. 2007 Mar 19;15(6):3348-60
19532576
J Biomed Opt. 2005 Mar-Apr;10(2):024013
15910087
J Biomed Opt. 2011 Feb;16(2):021103
21361666
Rev Sci Instrum. 2009 Aug;80(8):081101
19725639
Biomed Opt Express. 2010 Jul 14;1(1):59-73
21258446
Opt Commun. 2008 Apr 1;281(7):1813-1822
19343073
Appl Opt. 2010 Sep 1;49(25):F10-7
20820199
Photosynth Res. 2009 Nov-Dec;102(2-3):111-41
19851883