Interactions between laminin receptor and the cytoskeleton during translation and cell motility.
Human laminin receptor acts as both a component of the 40S ribosomal subunit to mediate cellular translation and as a cell surface receptor that interacts with components of the extracellular matrix. Due to its role as the cell surface receptor for several viruses and its overexpression in several types of cancer, laminin receptor is a pathologically significant protein. Previous studies have determined that ribosomes are associated with components of the cytoskeleton, however the specific ribosomal component(s) responsible has not been determined. Our studies show that laminin receptor binds directly to tubulin. Through the use of siRNA and cytoskeletal inhibitors we demonstrate that laminin receptor acts as a tethering protein, holding the ribosome to tubulin, which is integral to cellular translation. Our studies also show that laminin receptor is capable of binding directly to actin. Through the use of siRNA and cytoskeletal inhibitors we have shown that this laminin receptor-actin interaction is critical for cell migration. These data indicate that interactions between laminin receptor and the cytoskeleton are vital in mediating two processes that are intimately linked to cancer, cellular translation and migration.
Gene Therapy Center, Cancer Institute and Department of Pathology, New York University School of Medicine, New York, New York, United States of America.
Lisa
L
Venticinque
Kelly V
KV
Jamieson
Daniel
D
Meruelo
D000199:Actins / Q000378:metabolism; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D046912:Multiprotein Complexes; D011485:Protein Binding; D014176:Protein Biosynthesis; D017474:Receptors, Laminin / Q000378:metabolism* / Q000502:physiology; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D046912:Multiprotein Complexes; D017474:Receptors, Laminin; D014404:Tubulin
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