The function and mechanism of COX-2 in angiogenesis of gastric cancer cells.
BACKGROUND
Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells.


METHODS
The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2.


RESULTS
Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN.


CONCLUSIONS
COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.
State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 15 West Changle Road, Xi'an, 710032, PR China.
Liping
L
Yao
Fei
F
Liu
Liu
L
Hong
Li
L
Sun
Shuhui
S
Liang
Kaichun
K
Wu
Daiming
D
Fan
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000235:genetics; D009389:Neovascularization, Pathologic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms; D014471:Umbilical Veins / Q000166:cytology
D034741:RNA, Small Interfering; D051546:Cyclooxygenase 2
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