Pyrococcus yayanosii sp. nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent.
An obligate piezophilic anaerobic hyperthermophilic archaeon, designated strain CH1(T), was isolated from a hydrothermal vent site named 'Ashadze', which is located on the Mid-Atlantic Ridge at a depth of 4100 m. Enrichment and isolation of the strain were carried out at 95 °C under a hydrostatic pressure of 42 MPa. Cells of strain CH1(T) were highly motile irregular cocci with a diameter of ~1-1.5 µm. Growth was recorded at 80-108 °C (optimum 98 °C) and at pressures of 20-120 MPa (optimum 52 MPa). No growth was observed under atmospheric pressures at 60-110 °C. Growth was observed at pH 6.0-9.5 (optimum 7.5-8.0) and in 2.5-5.5% (w/v) NaCl (optimum 3.5%). Strain CH1(T) was strictly anaerobic and grew on complex proteinaceous substrates, such as yeast extract, Peptone, and casein, as well as on sucrose, starch, chitin, pyruvate, acetate and glycerol without electron acceptors. The G+C content of the genomic DNA was 49.0±0.5 mol%. Analysis of 16S rRNA gene sequences revealed that strain CH1(T) belongs to the genus Pyrococcus. Based on its physiological properties and similarity levels between ribosomal proteins, strain CH1(T) represents a novel species, for which the name Pyrococcus yayanosii sp. nov. is proposed. The type strain is CH1(T) (=JCM 16557). This strain is also available by request from the Souchothèque de Bretagne (catalogue LMBE) culture collection (collection no. 3310).
Université Européenne de Bretagne (UEB), 5 Boulevard René Laennec, F-35000 Rennes, France.|CNRS, IUEM - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.|Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la Mer) - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.
Jean-Louis
JL
Birrien
3rd Institute of Oceanography, SOA, Xiamen University, 361005 Xiamen, PR China.|Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la Mer) - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.
Xiang
X
Zeng
Université Européenne de Bretagne (UEB), 5 Boulevard René Laennec, F-35000 Rennes, France.|CNRS, IUEM - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.|Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la Mer) - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.
Mohamed
M
Jebbar
Ifremer, UMR 6197, Technopole Brest-Iroise, BP 70, F-29280 Plouzané, France.
Marie-Anne
MA
Cambon-Bonavita
Ifremer, UMR 6197, Technopole Brest-Iroise, BP 70, F-29280 Plouzané, France.
Joël
J
Quérellou
Laboratoire de Sciences de la Terre, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex, France.|CNRS, UMR 5570, Laboratoire de Sciences de la Terre, 15 parvis René Descartes BP 7000, Lyon, F-69342, France.|Université de Lyon, Lyon, France.
Philippe
P
Oger
Université Européenne de Bretagne (UEB), 5 Boulevard René Laennec, F-35000 Rennes, France.|CNRS, IUEM - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.|Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la Mer) - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.
Nadège
N
Bienvenu
School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
Xiang
X
Xiao
Université Européenne de Bretagne (UEB), 5 Boulevard René Laennec, F-35000 Rennes, France.|CNRS, IUEM - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.|Université Bretagne Occidentale (UBO), IUEM (Institut Universitaire Européen de la Mer) - UMR 6197, Technopole Brest-Iroise, place Nicolas Copernic, F-29280 Plouzané, France.
Daniel
D
Prieur
D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D019711:Pyrococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride