Single-step kinase inhibitor screening using a peptide-modified gold nanoparticle platform.
Two complementary formats for kinase inhibitor screening are presented in which peptide-modified gold nanoparticles are enzymatically phosphorylated and rapidly aggregate on a surface or in solution by action of phosphospecific antibodies. The simple and rapid colourimetric response of the assays makes them an attractive approach for drug-screening applications.
Imperial College London, Department of Materials and Institute for Biomedical Engineering, Prince Consort Road, London, SW7 2AZ, UK.
Shalini
S
Gupta
Heiko
H
Andresen
Molly M
MM
Stevens
D000906:Antibodies / Q000276:immunology; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000737:chemistry*
D000906:Antibodies; D010748:Phosphopeptides; D047428:Protein Kinase Inhibitors; D006046:Gold