Mapping proteoglycan-bound water in cartilage: Improved specificity of matrix assessment using multiexponential transverse relaxation analysis.
Association of MR parameters with cartilage matrix components remains an area of ongoing investigation. Multiexponential analysis of nonlocalized transverse relaxation data has previously been used to quantify water compartments associated with matrix macromolecules in cartilage. We extend this to mapping the proteoglycan (PG)-bound water fraction in cartilage, using mature and young bovine nasal cartilage model systems, toward the goal of matrix component-specific imaging. PG-bound water fraction from mature and young bovine nasal cartilage was 0.31 ± 0.04 and 0.22 ± 0.06, respectively, in agreement with biochemically derived PG content and PG-to-water weight ratios. Fourier transform infrared imaging spectroscopic-derived PG maps normalized by water content (IR-PG(ww) ) showed spatial correspondence with PG-bound water fraction maps. Extensive simulation analysis demonstrated that the accuracy and precision of our determination of PG-bound water fraction was within 2%, which is well-within the observed tissue differences. Our results demonstrate the feasibility of performing imaging-based multiexponential analysis of transverse relaxation data to map PG in cartilage.
Magnetic Resonance Imaging and Spectroscopy Section, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA. reiterda@mail.nih.gov
David A
DA
Reiter
Remigio A
RA
Roque
Ping-Chang
PC
Lin
Onyi
O
Irrechukwu
Stephen
S
Doty
Dan L
DL
Longo
Nancy
N
Pleshko
Richard G
RG
Spencer
D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055171:Nasal Cartilages / Q000378:metabolism*; D010329:Patella; D011509:Proteoglycans / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared
D011509:Proteoglycans
Magn Reson Med. 2000 Jan;43(1):34-44
10642729
Magn Reson Imaging. 2004 Jan;22(1):67-80
14972396
Invest Radiol. 2000 Oct;35(10):622-38
11041156
Magn Reson Med. 1996 Mar;35(3):370-8
8699949
J Biol Chem. 1977 Jun 10;252(11):3617-25
140875
J Magn Reson Imaging. 2003 Apr;17(4):440-4
12655583
Magn Reson Imaging. 2001 Dec;19(10):1279-86
11804755
Magn Reson Med. 2005 Sep;54(3):524-31
16086363
Magn Reson Med. 2009 Apr;61(4):803-9
19189393
Mult Scler. 2006 Dec;12(6):747-53
17263002
Magn Reson Med. 1991 Oct;21(2):264-81
1745125
Ann Rheum Dis. 1979 Aug;38(4):371-7
496451
Cells Tissues Organs. 2003;175(3):121-32
14663155
J Magn Reson. 2009 Feb;196(2):149-56
19028432
Biomaterials. 2007 May;28(15):2465-78
17175021
Magn Reson Med. 2004 Mar;51(3):503-9
15004791
J Orthop Res. 1992 Jan;10(1):1-13
1309384
Biopolymers. 2001;62(1):1-8
11135186
J Orthop Res. 2004 May;22(3):557-64
15099635
Magn Reson Med. 2006 Mar;55(3):532-40
16450338
Biochim Biophys Acta. 1986 Sep 4;883(2):173-7
3091074
Clin Biochem. 1996 Jun;29(3):225-9
8740508
Magn Reson Med. 2002 Feb;47(2):284-91
11810671
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2266-70
18268341
Magn Reson Med. 2009 Nov;62(5):1311-8
19705467
Biomaterials. 2000 Oct;21(20):2089-95
10966019
Magn Reson Med. 1997 Oct;38(4):669-77
9324335
Neurology. 2000 Nov 28;55(10):1506-10
11094105
J Magn Reson. 2009 Nov;201(1):61-71
19762258