Lentiviral vectors targeted to MHC II are effective in immunization.
Abstract vectors (LVs) that are targeted to APC using a chimeric measles virus (MV) hemagglutinin (H). The MV H protein is mutated to prevent binding to MV receptors and incorporates a single-chain antibody that recognizes murine major histocompatibility complex class II (MHC II). This targeted LV is highly efficient in transduction of freshly isolated mouse B cells and dendritic cells. MHC II-positive cells in spleen are transduced after intravenous injection, and a robust immune response to an antigen transgene is generated.
MRC Medical Molecular Virology Centre, Division of Infection and Immunity, University College London, United Kingdom.
Alexander
A
Ageichik
Christian J
CJ
Buchholz
Mary K
MK
Collins
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D058501:Enzyme-Linked Immunospot Assay; D005802:Genes, MHC Class II / Q000276:immunology*; D005822:Genetic Vectors / Q000276:immunology*; D006388:Hemagglutinins / Q000276:immunology; D007114:Immunization / Q000379:methods*; D016086:Lentivirus; D008459:Measles virus / Q000737:chemistry; D051379:Mice; D014161:Transduction, Genetic
D006388:Hemagglutinins