Simple surface modification of poly(dimethylsiloxane) for DNA hybridization.
Here, we present a simple chemical modification of poly(dimethylsiloxane) (PDMS) by curing a mixture of 2 wt% undecylenic acid (UDA) in PDMS prepolymer on a gold-coated glass slide. This gold slide had been previously pretreated with a self-assembled hydrophilic monolayer of 3-mercaptopropionic acid (MPA). During curing of the UDA∕PDMS prepolymer, the hydrophilic UDA carboxyl moieties diffuses toward the hydrophilic MPA carboxyl moieties on the gold surface. This diffusion of the UDA within the PDMS prepolymer to the surface is a direct result of surface energy minimization. Once completely cured, the PDMS is peeled off the gold substrate, thereby exposing the interfacial carboxyl groups. These groups are then available for subsequent attachment of 5(')-amino terminated DNA oligonucleotides via amide linkages. Our results show that the covalently tethered oligonucleotides can successfully capture fluorescein-labeled complementary oligonucleotides via hybridization, which are visualized using fluorescence microscopy.
Centre for NanoScale Science and Nanotechnology, School of Chemical and Physical Sciences, Flinders University, Adelaide SA 5001, Australia.
Jinwen
J
Zhou
Nicolas H
NH
Voelcker
Amanda V
AV
Ellis
J Chromatogr B Biomed Sci Appl. 2001 Oct 25;762(2):117-25
11678371
Anal Chem. 2006 Nov 1;78(21):7446-52
17073411
Anal Chem. 2005 Feb 1;77(3):785-96
15679345
Lab Chip. 2006 Jul;6(7):942-7
16804600
Electrophoresis. 2010 Jan;31(1):2-16
20039289
Anal Chem. 2006 Aug 1;78(15):5543-51
16878894
Anal Chem. 2006 Jul 1;78(13):4588-92
16808469
Anal Chem. 2008 Jun 1;80(11):4119-24
18457409
Anal Chem. 2005 Dec 1;77(23):7539-46
16316160
J Mater Sci Mater Med. 2001 Feb;12(2):111-9
15348316
Anal Chem. 2005 Mar 1;77(5):1414-22
15732926
Lab Chip. 2008 Apr;8(4):516-8
18369504
Anal Chem. 2004 Apr 1;76(7):2055-61
15053671
Anal Chem. 2005 Jul 1;77(13):3971-8
15987099
Biomed Microdevices. 2005 Dec;7(4):281-93
16404506
J Colloid Interface Sci. 2002 Oct 15;254(2):306-15
12702402
J Chromatogr A. 2008 Oct 17;1207(1-2):175-80
18783780
Anal Chem. 2005 Nov 1;77(21):6909-14
16255589
Talanta. 2007 Mar 30;71(5):2048-55
19071562
Langmuir. 2004 Oct 26;20(22):9607-11
15491193
Anal Chem. 2005 Jan 15;77(2):573-8
15649055
Colloids Surf B Biointerfaces. 2008 Jun 1;63(2):301-5
18242961
J Biomed Mater Res A. 2005 Jan 1;72(1):10-8
15534867
Electrophoresis. 2007 Dec;28(24):4597-605
18072225
Electrophoresis. 2005 Jan;26(1):211-8
15624173
Electrophoresis. 2007 Sep;28(18):3302-7
17854125
Electrophoresis. 2001 Oct;22(17):3736-43
11699912
J Sep Sci. 2008 Aug;31(15):2860-7
18655017
Electrophoresis. 2006 Jul;27(14):2933-9
16721904
J Am Chem Soc. 2006 Jan 11;128(1):374-80
16390168
Anal Sci. 2007 Dec;23(12):1409-14
18071227
Langmuir. 2006 Feb 28;22(5):2090-5
16489793
Langmuir. 2007 Apr 10;23(8):4465-71
17341100
Angew Chem Int Ed Engl. 2007;46(35):6634-7
17654758