Conjugation behaviours of CdTe quantum dots and antibody by a novel immunochromatographic method.
Three water-soluble CdTe quantum dots (QDs) (green-emitting, yellow-emitting and red-emitting) were synthesised for different refluxing time with 3-mercaptopropionic acid (MPA) as stabiliser. Then the red-emitting CdTe QDs and mouse immunoglobulin G (IgG) were taken as the representative to study the conjugation behaviour of QDs and antibody by a novel immunochromatographic method. After comparing with several methods, that is, direct conjugation, 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC)-mediated conjugation, N-hydroxysuccinimide (NHS)-mediated conjugation, EDC/NHS-mediated conjugation by immunochromatographic strips, EDC and NHS were selected together as coupling agents to conjugate QDs with antibody efficiently. Finally, the K562 leukaemia cells were incubated with the EDC/NHS-mediated conjugates to evaluate the performance in practical application, and the result from fluorescence images showed that it was successfully applied to label cells. The immunochromatographic strip was a superior method to study the conjugation of the fluorophore and antibody.
Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, Shanghai, People's Republic of China.
Y
Y
Wang
Y
Y
Bai
X
X
Wei
D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000378:metabolism; D019187:Cadmium Compounds / Q000737:chemistry* / Q000378:metabolism; D002234:Carbodiimides / Q000737:chemistry; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006651:Histocytochemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013388:Succinimides / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry* / Q000378:metabolism
C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D055910:Antibodies, Immobilized; D019187:Cadmium Compounds; D002234:Carbodiimides; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D013388:Succinimides; C001426:N-hydroxysuccinimide; D013691:Tellurium; C028337:cadmium telluride