DddY, a periplasmic dimethylsulfoniopropionate lyase found in taxonomically diverse species of Proteobacteria.
The abundant compatible solute dimethylsulfoniopropionate (DMSP) is made by many marine algae. Different marine bacteria catabolise DMSP by various mechanisms, some of which liberate the environmentally important gas dimethyl sulfide (DMS). We describe an enzyme, DddY, which cleaves DMSP into DMS plus acrylate and is located in the bacterial periplasm, unlike other DMSP lyases that catalyse this reaction. There are dddY-like genes in strains of Alcaligenes, Arcobacter and Shewanella, in the β-, ɛ- and γ-proteobacteria, respectively. In Alcaligenes, dddY is in a cluster of ddd and acu genes that resemble, but also have significant differences to, those in other bacteria that catabolise both DMSP and acrylate. Although production of DMS and transcription of Alcaligenes dddY are both apparently inducible by pre-growth of cells with DMSP, this substrate must be catabolised to form acrylate, the bona fide coinducer.
School of Biological Sciences, University of East Anglia, Norwich, UK.
Andrew R J
AR
Curson
Matthew J
MJ
Sullivan
Jonathan D
JD
Todd
Andrew W B
AW
Johnston
D000179:Acrylates / Q000378:metabolism; D000421:Alcaligenes / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D013437:Carbon-Sulfur Lyases / Q000235:genetics*; D019295:Computational Biology; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D020560:Proteobacteria / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D013440:Sulfides / Q000378:metabolism; D013452:Sulfonium Compounds / Q000378:metabolism
D000179:Acrylates; D001426:Bacterial Proteins; D013440:Sulfides; D013452:Sulfonium Compounds; C068078:dimethylpropiothetin; D013437:Carbon-Sulfur Lyases; C400924:dimethylsulfoniopropionate lyase; C036658:acrylic acid; C004784:dimethyl sulfide
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