Expression of CD64 (FcγRI) in skin of patients with acute GVHD.
GVHD remains a major problem in allo-SCT. We explored the presence of APC in skin biopsies of GVHD patients, using the IgG receptor CD64 expression as a hallmark for activated APC. By immunohistochemistry we demonstrated CD64 to be upregulated on host APC in skin biopsies of patients with acute GVHD and, less prominently, in chronic GVHD. Double staining for CD32 polymorphism revealed CD64-positive cells to be mainly of host origin. The majority of CD64-positive cells coexpressed CD68, indicating a macrophage phenotype. Given its very restricted cellular distribution, CD64 may represent an excellent target for APC-directed therapies in GVHD.
Immunotherapy Laboratory, Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
A
A
van Royen-Kerkhof
V
V
Walraven
E A M
EA
Sanders
R
R
de Weger
D F
DF
van Wichen
E
E
de Koning
T
T
Thepen
J G J
JG
van de Winkel
J H W
JH
Leusen
D000208:Acute Disease; D000938:Antigen-Presenting Cells / Q000378:metabolism / Q000473:pathology; D002908:Chronic Disease; D005260:Female; D005786:Gene Expression Regulation; D006086:Graft vs Host Disease / Q000378:metabolism* / Q000473:pathology; D019337:Hematologic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D006801:Humans; D008297:Male; D017452:Receptors, IgG / Q000096:biosynthesis*; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D012871:Skin Diseases / Q000378:metabolism* / Q000473:pathology; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous
D017452:Receptors, IgG