Oligonucleotide labeling using BODIPY phosphoramidite.
4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives were prepared and their photochemical properties were characterized. One such analogue, 4,4-difluoro-4-bora-1,3,5,7-tetramethyl-8-(5-hydroxypentyl)-3a,4a-diaza-s-indacene was transformed into the corresponding phosphoramidite and incorporated into oligodeoxyribonucleotides as a fluorescent reporter group.
Department of Chemistry, Brock University, St. Catharines, Ontario, Canada.
Kha
K
Tram
Daniel
D
Twohig
Hongbin
H
Yan
D001896:Boron Compounds / Q000032:analysis / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis / Q000737:chemistry*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite