The melanoma-associated antigen-A3, -A4 genes: relation to the risk and clinicopathological parameters in breast cancer patients.
This study aimed to evaluate the clinical reliability and accuracy of two MAGE transcripts (MAGE-A3, MAGE-A4 mRNA) in the peripheral blood (PB) of patients with breast cancer (BC), and to evaluate their potential limits and utility to detect BC. We aimed also to analyze their relation to clinicopathological characteristics of the tumor. This study is a prospective, controlled, double-blinded study conducted on 100 BC women and 100 age-matched control women. There were 52 patients with localized breast mass with no evidence of nodal affection or distant metastases and 48 patients suffering from metastatic BC. MAGE-A3 and MAGE-A4 mRNA in the PB were assayed using reverse transcriptase-polymerase chain reaction (RT-PCR). None of the control women was positive for either MAGE-A3, MAGE-A4. In BC women, positivity for MAGE-A3 in PB was observed in 37 patients (37%), and MAGE-A4 positivity was observed in 11 patients (11%); with 100% specificity for both transcripts. The presence of MAGE-A3 was significantly associated with nodal status (P = 0.009), tumor size (P = 0.009), and American Joint Committee on Cancer stage (P = 0.009), whereas MAGE-A4 positivity was significantly associated with histological grade (P = 0.020). RT-PCR assays of MAGE-A3 and MAGE-A4 in the PB of BC patients may have prognostic and predictive implications, and they are promising specific tumor markers of BC.
Department of Medical Biochemistry, Zagazig University, Zagazig 44519, Egypt.
Yousri M
YM
Hussein
Amal F
AF
Gharib
Rasha L
RL
Etewa
Amal S
AS
El-Shal
Mohamed Esmat
ME
Abdel-Ghany
Wael H
WH
Elsawy
D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D017931:DNA Primers; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017931:DNA Primers; C072013:MAGEA3 protein, human; C095204:MAGEA4 protein, human; D009363:Neoplasm Proteins; D012333:RNA, Messenger
Mol Cell Biol. 2004 Jun;24(11):4781-90
15143172
J Cell Physiol. 2003 Mar;194(3):272-88
12548548
Br J Cancer. 2005 May 23;92(10):1948-52
15841077
Cancer. 2002 Jun 25;96(3):187-93
12115308
Ann Oncol. 2001 Dec;12(12):1693-8
11843246
ORL J Otorhinolaryngol Relat Spec. 2004;66(6):332-40
15668533
J Clin Oncol. 1998 Aug;16(8):2632-40
9704713
Trends Cell Biol. 2006 May;16(5):229-33
16581249
Nat Med. 2000 Jan;6(1):96-9
10613832
Nat Rev Cancer. 2005 Aug;5(8):615-25
16034368
Cancer Res. 2001 Dec 15;61(24):8845-50
11751407
Nat Rev Clin Oncol. 2009 Jun;6(6):339-51
19399023
J Natl Cancer Inst. 1999 Jul 7;91(13):1113-24
10393719
Leuk Res. 2007 Jan;31(1):33-7
16806467
J Clin Oncol. 2000 May;18(9):1914-20
10784632
Cell Cycle. 2005 Oct;4(10):1335-7
16177571
CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49
19474385
Acta Biochim Pol. 2004;51(3):747-55
15448736
Lancet. 2005 May 14-20;365(9472):1687-717
15894097
Ann Oncol. 1997 Apr;8(4):369-72
9209667
J Biol Chem. 2006 Apr 14;281(15):10118-26
16497664
Breast Cancer Res Treat. 2001 Mar;66(1):59-66
11368411
Cancer Res. 2005 Jun 1;65(11):4747-53
15930293
Croat Med J. 2006 Feb;47(1):32-41
16489695
J Clin Oncol. 2003 Jul 1;21(13):2609-15
12829682
Br J Cancer. 2001 Nov 2;85(9):1340-6
11720472
J Biol Chem. 2003 Mar 21;278(12):10668-74
12525503
J Clin Oncol. 1995 Nov;13(11):2769-75
7595737
Med Sci Monit. 2009 Feb;15(2):MT34-40
19179977
Liver. 1999 Apr;19(2):110-4
10220740
J Korean Med Sci. 2000 Apr;15(2):194-8
10803697
Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7149-53
8692960
Cancer Res. 2002 Jul 15;62(14):3971-9
12124329
J Immunother. 2004 Sep-Oct;27(5):329-38
15314541
Br J Cancer. 1957 Sep;11(3):359-77
13499785
Br J Cancer. 2004 Feb 23;90(4):838-43
14970862
Oncol Rep. 2004 Jul;12(1):145-51
15201976
Cancer Res. 2001 Sep 15;61(18):6682-7
11559535
Med Sci Monit. 2007 Nov;13(11):BR231-239
17968289
Cancer Immun. 2004 Jan 23;4:1
14738373
Clin Cancer Res. 2008 Jun 1;14(11):3291-8
18519755
Med Sci Monit. 2008 Mar;14(3):CR136-43
18301357