Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers.
Hybrid identification of 16 sunflower hybrids was confirmed using simple-sequence repeat methodology. Of 20 specific simple-sequence repeat primers, 18 authenticated the purity of these hybrids; the remaining two specific primer pairs gave ambiguous DNA fragments. The results indicate that simple-sequence repeat analysis for the identification of hybrids derived from the crossing of different inbred sunflower lines can improve the accuracy of selection, save time and reduce cost.
Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan. ahsanuaf2003@yahoo.com
A
A
Iqbal
H A
HA
Sadaqat
A S
AS
Khan
M
M
Amjad
D002678:Chimera / Q000235:genetics; D018744:DNA, Plant; D005819:Genetic Markers; D006368:Helianthus / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D018744:DNA, Plant; D005819:Genetic Markers