Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum.
Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog (MSPH), enolase (Eno), bilin-binding protein (BBP), imaginal disc growth factor (IDGF), ornithine decarboxylase (ODC), cellular retinoic acid binding protein (CRABP), and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoid-induced host alterations associated with innate immune responses, detoxification, and energy metabolism.
State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China.
Jia-ying
JY
Zhu
Qi
Q
Fang
Gong-yin
GY
Ye
Cui
C
Hu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D017344:Genes, Insect; D006790:Host-Parasite Interactions / Q000235:genetics / Q000502:physiology; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007915:Lepidoptera / Q000469:parasitology*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D020543:Proteome / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014863:Wasps / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*
D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D021382:Protein Sorting Signals; D020543:Proteome; D012333:RNA, Messenger
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