A Novel Approach for Oral Delivery of Insulin via Desmodium gangeticum Aqueous Root Extract.
Many challenges are associated with the oral delivery of insulin, relating to the physical and chemical stability of the hormone, and its absorption and metabolism in the human body. The present study aims to demonstrate the oral delivery of insulin in both normal and steptozotocin (STZ)-induced diabetic rats with the help of the aqueous extract of Desmodium gangeticum (DG) root. Human insulin was mixed with the aqueous extract of DG root (0.1 mg/ml) with human insulin (40 IU/ml) in ratio 1:1(v/v), to prepare oral insulin drug. Decreased plasma glucose level and increased plasma insulin in normal and STZ-induced diabetic rat suggested the probable absorption of insulin through GI tract when insulin was administered by mixing with DG extract. Indeed, insulin mixed DG potentially stimulates the release of insulin in STZ-induced diabetic rat rather than in normal animal. In vivo insulin secretaguage action of oral insulin drug was determined by isolated rat heart model and the results showed a significant cardio protection in STZ rat. The finding of this study suggests that insulin mixed with DG extract can be a promising vehicle for oral delivery of insulin. However, further studies are required to explore the exact compound(s) responsible for the protective delivery of insulin orally. Increased plasma insulin level by insulin mixed DG extract administration in STZ-treated diabetic rat indicates not only insulin secretaguage action of the mixture but also a probable altered insulin release mechanism in diabetic condition.
School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu-613 402, India.
Ga
G
Kurian
Av
A
Seetharaman
Nr
N
Subramanian
J
J
Paddikkala
Planta Med. 2007 May;73(5):427-32
17431824
Arch Neurol. 1961 May;4:520-5
13730599
Clin Chem. 1972 Sep;18(9):943-50
5052095
Methods Find Exp Clin Pharmacol. 1988 Oct;10(10):645-56
3070203
J Clin Pathol. 1962 Jan;15:57-61
14449023
Biol Pharm Bull. 2005 Apr;28(4):729-32
15802818
J Ocul Pharmacol Ther. 1996 Winter;12(4):515-26
8951688
Tissue Cell. 1992;24(5):603-11
1440582
Horm Metab Res Suppl. 1985;15:111-5
3865878
Diabetes. 1996 Apr;45(4):454-62
8603767
Eur J Pharm Sci. 2007 Apr;30(5):392-7
17280820
J Thorac Cardiovasc Surg. 2002 Jun;123(6):1120-4
12063458
Cardiovasc Diabetol. 2006 Dec 04;5:26
17144912
Science. 1986 Sep 5;233(4768):1081-4
3526553
Chem Pharm Bull (Tokyo). 1996 Jan;44(1):235-6
8582042
Biochem Soc Trans. 1975;3(3):410-4
124280
Skin Pharmacol Appl Skin Physiol. 1999 Jul-Aug;12(4):221-6
10420142
Bioorg Med Chem Lett. 2005 Oct 15;15(20):4543-6
16099649
Int J Pharm. 2008 Jan 22;347(1-2):149-55
17707601