An influenza hemagglutinin A peptide assay based on the enzyme-multiplied immunoassay technique.
A practical approach for constructing enzyme-multiplied immunoassay technique (EMIT)-based protein/peptide assays is described. Normally used in small-molecule drug testing, EMIT is a homogeneous assay method that is attractive for its simplicity, sensitivity, and rapidity. The EMIT-based peptide/protein assay was developed by conjugating a cysteine-modified HA peptide (from influenza hemagglutinin A) to the reporter enzyme, glucose-6-phosphate dehydrogenase. The 13-min assay gave a free HA limit of detection of 10 nM and proved effective for detection of a high-molecular-weight model protein tagged with HA. Similar EMIT-based assay approaches may be developed for applications in biotoxin and infectious disease detection.
Chemical and Biomolecular Engineering Department, University of California-Los Angeles, CA 90095, USA.
May L
ML
Chiu
Denton
D
Lai
Harold G
HG
Monbouquette
D002138:Calibration; D017248:Enzyme Multiplied Immunoassay Technique; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis* / Q000737:chemistry
D019267:Hemagglutinin Glycoproteins, Influenza Virus; C483468:hemagglutinin, human influenza A virus; D005954:Glucosephosphate Dehydrogenase