Changes in corneal basal epithelial phenotypes in an altered basement membrane.
BACKGROUND
To examine the corneal epithelial phenotype in an altered basement membrane.


RESULTS
Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (ΔNp63), cytokeratin 3 (Krt3), ATP-binding cassette sub-family G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and ΔNp63 were detected in the full-thickness epithelial cells of CUCC patients.


CONCLUSIONS
Our results indicate that alteration of the corneal basement membrane induces a de-differentiation-like phenotype in corneal basal epithelial cells.
Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
I-Jong
IJ
Wang
Ray Jui-Fang
RJ
Tsai
Lung-Kun
LK
Yeh
Ryan Yao-Nien
RY
Tsai
Fung-Rong
FR
Hu
Winston W Y
WW
Kao
D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology*; D054337:Cell Dedifferentiation; D003315:Cornea; D004847:Epithelial Cells / Q000737:chemistry / Q000473:pathology; D019573:Epithelium, Corneal / Q000737:chemistry / Q000473:pathology*; D005136:Eye Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D015948:Keratoplasty, Penetrating; D010641:Phenotype
D005136:Eye Proteins
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