Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager.
Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires extremely low light level operation typical of biological analyte concentrations, long data acquisition periods and on-chip processing capability to realize these advantages. We report here fluorescence lifetime data obtained using a CMOS-SPAD imager in conjunction with DNA microarrays and TIRF excitation geometry. This enables acquisition of single photon arrival time histograms for a 320 pixel FLIM map within less than 26 seconds exposure time. From this, we resolve distinct lifetime signatures corresponding to dye-labelled HCV and quantum-dot-labelled HCMV nucleic acid targets at concentrations as low as 10 nM.
Gerard
G
Giraud
Holger
H
Schulze
Day-Uei
DU
Li
Till T
TT
Bachmann
Jason
J
Crain
David
D
Tyndall
Justin
J
Richardson
Richard
R
Walker
David
D
Stoppa
Edoardo
E
Charbon
Robert
R
Henderson
Jochen
J
Arlt
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