The effect of layer-by-layer chitosan-hyaluronic acid coating on graft-to-bone healing of a poly(ethylene terephthalate) artificial ligament.
Surface coating with an organic layer-by-layer self-assembled template of chitosan and hyaluronic acid on a poly(ethylene terephthalate) (PET) artificial ligament was designed for the promotion and enhancement of graft-to-bone healing after artificial ligament implantation in a bone  tunnel. The results of in vitro culturing of MC3T3-E1 mouse osteoblastic cells supported the hypothesis that the layer-by-layer coating of chitosan and hyaluronic acid could promote the cell compatibility of grafts and could promote osteoblast proliferation. A rabbit extra-articular  tendon-to-bone healing model was used to evaluate the effect of this kind of surface-modified stainless artificial ligament in vivo. The final results proved that this organic compound coating could significantly promote and enhance new bone formation at the graft-bone interface  histologically and, correspondingly, the experimental group with coating had significantly higher biomechanical properties compared with controls at 8 weeks (P < 0.05).
Department of Sports Medicine, Huashan Hospital, 12 Wulumuqi Zhong Road, Shanghai 200040, P. R. China.
Hong
H
Li
Yunsheng
Y
Ge
Pengyun
P
Zhang
Lingxiang
L
Wu
Shiyi
S
Chen
D016475:3T3 Cells; D000818:Animals; D016025:Bone Transplantation / Q000009:adverse effects*; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology / Q000601:surgery*; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008022:Ligaments / Q000187:drug effects* / Q000473:pathology; D008297:Male; D055595:Mechanical Phenomena; D051379:Mice; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties; D014945:Wound Healing / Q000187:drug effects
D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D006820:Hyaluronic Acid; D048271:Chitosan