Rhizoremediation of lindane by root-colonizing Sphingomonas.
We used a two-step enrichment approach to isolate root-colonizing hexachlorocyclohexane (HCH)-degrading microorganisms. The first step consists of the use of classical liquid enrichment to isolate γ-HCH degraders. The γ-HCH-degrading microbes were attached in mass to corn seeds sown in soil with γ-HCH, and after plant development we rescued bacteria growing on root tips. Bacteria were then subjected to a second enrichment round in which growth on liquid medium with γ-HCH and inoculation of corn seeds were repeated. We then isolated bacteria on M9 minimal medium with γ-HCH from root tips. We were able to isolate four Sphingomonas strains, all of which degraded α-, β-, γ- and δ-HCH. Two of the strains were particularly good colonizers of corn roots, reaching high cell density in vegetated soil and partly removing γ-HCH. In contrast, these bacteria performed poorly in unplanted soils. This study supports the hypothesis that the removal of persistent toxic chemicals can be accelerated by combinations of plants and bacteria, a process generally known as rhizoremediation.
Consejo Superior de Investigaciones Cientificas, Eslación Experimental del Zaidin, Department of Environmental Protection, C/ Prof. Albareda, 1, E-18008 Granada, Spain.
Dietmar
D
Böltner
Patricia
P
Godoy
Jesús
J
Muñoz-Rojas
Estrella
E
Duque
Silvia
S
Moreno-Morillas
Lourdes
L
Sánchez
Juan Luis
JL
Ramos
D001673:Biodegradation, Environmental; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D058441:Rhizosphere; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D020578:Sphingomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D003313:Zea mays / Q000382:microbiology
D007306:Insecticides; D012989:Soil Pollutants; D001556:Hexachlorocyclohexane
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