[Effect of decoction method on pharmacokinetics of puerarin in dogs].
OBJECTIVE
To investigate the effect of decoction method on pharmacokinetics of Puerarin by determining Puerarin concentration of serum after administration of Gegenqinlian in single (D) and mixed decoction (H) to dogs.


METHODS
HPLC method was established to determine serum concentration of Puerarin and pharmacokinetic parameters were calculated.


RESULTS
The parameters in dogs after administration of H and D were as follows: C(max) was (5.164 +/- 0.637) and (5.383 +/- 0.818) microg/mL, t(max) was (1.10 +/- 0.374) and (1.25 +/- 0.274) h, AUC(0-t) was (25.76 +/- 5.48) and (27.97 +/- 7.16) microg/(h x mL), respectively.


CONCLUSIONS
Decoction method has significant effect on pharmacokinetic parameters of puerarin in dogs.
Heilongjiang University of Chinese Medicine, Harbin 150076, China. kurb@sohu.com
Li
L
Wang
Ying
Y
Zhao
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry; D004285:Dogs; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D005260:Female; D006358:Hot Temperature; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D018517:Plant Roots / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*; D015203:Reproducibility of Results; D031363:Scutellaria / Q000737:chemistry; D013997:Time Factors; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D014665:Vasodilator Agents; C542794:gegenqinlian; C033607:puerarin