Enhanced gene transduction into skeletal muscle of mice in vivo with pluronic block copolymers and ultrasound exposure.
The pluronic block copolymers are able to enhance the ultrasound-induced gene delivery in vitro. In the present study, the effects of pluronics on the efficiency of gene transfer into skeletal muscle in vivo under sonoporation were investigated. Plasmid DNA encoding green fluorescent protein (GFP) in combination with three different pluronics, F127, L61, and P85, was injected into the tibialis anterior (TA) muscle of mice with and without adjunct ultrasound (1 MHz, 3 W/cm(2) 1 min, 20% duty cycle). Mice were killed 1 week after injection. The TA muscles were removed and snap frozen immediately in isopentane cooled by liquid nitrogen and sections of 7 μm thick were cut. Transfection efficiency was assessed by counting the number of GFP-positive fibers under fluorescence microscopy, and tissue damage by hematoxylin and eosin staining. The results suggested that all three pluronics significantly enhanced transgene expression in skeletal muscle (P < 0.01), especially the P85 showed significantly higher efficiency than the other two pluronics (P < 0.05). Ultrasound synergistically enhanced the gene delivery efficiency with P85 (P < 0.01), but was unable to do so with F127 and L61 groups. In short, P85 displays significantly synergistic effect with ultrasound for enhancing plasmid DNA transduction in skeletal muscle of mice in vivo.
Ultrasound Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Road, Wuhan 430030, China. ycchen@tjh.tjmu.edu.cn
Yun-Chao
YC
Chen
Li-Ping
LP
Jiang
Na-Xiang
NX
Liu
Lu
L
Ding
Xiao-Ling
XL
Liu
Zhi-Hui
ZH
Wang
Kai
K
Hong
Qing-Ping
QP
Zhang
D000818:Animals; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D020442:Poloxamer / Q000494:pharmacology* / Q000633:toxicity; D011095:Polyethylenes / Q000494:pharmacology; D011126:Polypropylenes / Q000494:pharmacology; D014161:Transduction, Genetic; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics
D011095:Polyethylenes; D011126:Polypropylenes; D020442:Poloxamer; D049452:Green Fluorescent Proteins; C078661:UCON 50-HB-5100