[Effects of applying different nitrogen form on cherry tomato nitrogen metabolism during fruit development].
A hydroponic experiment was conducted to study the effects of applying 100% NO3- -N, 100% NH4+ -N, and 75% NO3- -N+25% NH4+ -N on the nitrogen metabolism and the nitrate reductase (NR) and glutamine synthetase (GS) gene expression of cherry tomato during its fruit development. Applying 75% NO3- -N+25% NH4+ -N slightly increased the single fruit mass, and increased the fruit NH4+ -N, total amino acid, and total N contents and N accumulation significantly, compared with applying 100% NO3- -N. In treatments 100% NO3- -N and 75% NO3- -N + 25% NH4+ -N, the fruit NR activity and its gene expression had no significant difference, but were higher than those in treatment 100% NH4+ -N. The fruit GS activity was significantly higher in treatment 75% NO3--N+25% NH4+ -N than in treatment 100% NO3- -N. In the three treatments, isozyme GS1 (Cytosolic type GS) and GS2 (Chloroplast type GS) expression was inconsistent with GS activity, suggesting that the effects of applied N on GS activity could be mainly reflected at posttranscriptional level.
College of Resources and Environmental Science, Nanjing Agricultural University, China. 2007103110@njau.edu.cn
Qing-yu
QY
Li
Xin-juan
XJ
Xu
Hai-long
HL
Gu
Hong-yan
HY
Gao
Yi-yong
YY
Zhu
Cai-xia
CX
Dong
Qi-rong
QR
Shen
D046508:Culture Techniques / Q000379:methods; D005308:Fertilizers; D005638:Fruit / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry* / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D005308:Fertilizers; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen