Comparative in vivo antiangiogenic effects of calreticulin from Trypanosoma cruzi and Homo sapiens sapiens.
Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the fact that the observed TcCRT effects can be attributed to the parasite-derived molecule and not to the endotoxin. The antiangiogenic TcCRT effects correlate with its anti-tumor in vivo effects, as recently shown in our laboratory.
Laboratory of Immunology of Microbial Aggression, Immunology Disciplinary Program, ICBM, Faculty of Medicine, University of Chile, Independence, Santiago, Chile.
Victor
V
Toledo
Galia
G
Ramírez
Carolina
C
Valck
Nandy
N
López
Carolina H
CH
Ribeiro
Ismael
I
Maldonado
Lorena
L
Aguilar
David
D
Lemus
Arturo
A
Ferreira
D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D037282:Calreticulin / Q000302:isolation & purification / Q000494:pharmacology*; D002642:Chick Embryo; D006801:Humans; D009389:Neovascularization, Pathologic; D014349:Trypanosoma cruzi / Q000737:chemistry*
D020533:Angiogenesis Inhibitors; D037282:Calreticulin