Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells.
Although the functions of adiponectin, a differentiated adipocyte-derived hormone, in regulating glucose and fatty acid metabolism are regulated by two subtypes of adiponectin receptors (AdipoRs; AdipoR1 and AdipoR2), those in ruminants remain unclear. Therefore we examined the messenger RNA (mRNA) expression levels of adiponectin and its receptors in various bovine tissues and mammary glands among different lactation stages, and the effects of lactogenic hormones (insulin, dexamethasone and prolactin) and growth hormone (GH) on mRNA expression of the AdipoRs in cultured bovine mammary epithelial cells (BMEC). AdipoRs mRNAs were widely expressed in various bovine tissues, but adiponectin mRNA expression was significantly higher in adipose tissue than in other tissues. In the mammary gland, although adiponectin mRNA expression was significantly decreased at lactation, AdipoR1 mRNA expression was significantly higher at peak lactation than at the dry-off stage. In BMEC, lactogenic hormones and GH upregulated AdipoR2 mRNA expression but did not change that of AdipoR1. In conclusion, adiponectin and its receptor mRNA were expressed in various bovine tissues and the adiponectin mRNA level was decreased during lactation. These results suggest that adiponectin and its receptors ware changed in mammary glands by lactation and that AdipoRs mRNA expression was regulated by different pathways in BMEC.
Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai, Japan.
Yoshihisa
Y
Ohtani
Tomo
T
Yonezawa
Sang-Houn
SH
Song
Tatsuyuki
T
Takahashi
Astrid
A
Ardiyanti
Katsuyoshi
K
Sato
Akihiko
A
Hagino
Sang-gun
SG
Roh
Kazuo
K
Katoh
D052242:Adiponectin / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology / Q000502:physiology; D007774:Lactation / Q000235:genetics / Q000502:physiology; D042361:Mammary Glands, Human / Q000166:cytology* / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D013806:Theophylline / Q000031:analogs & derivatives; D015854:Up-Regulation
D052242:Adiponectin; D007328:Insulin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; C073294:growth hormone, bovine; D003907:Dexamethasone; D011388:Prolactin; D013006:Growth Hormone; D013806:Theophylline; C006683:bamifylline