Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration.
Pseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered robust biofilm formation is not clearly defined yet. Here, we identified a morphological change naturally accompanied by anaerobic respiration in P. aeruginosa and investigated its effect on the biofilm formation in vitro. A standard laboratory strain, PAO1 was highly elongated during anaerobic respiration compared with bacteria grown aerobically. Microscopic analysis demonstrated that cell elongation likely occurred as a consequence of defective cell division. Cell elongation was dependent on the presence of nitrite reductase (NIR) that reduces nitrite (NO(2) (-)) to nitric oxide (NO) and was repressed in PAO1 in the presence of carboxy-PTIO, a NO antagonist, demonstrating that cell elongation involves a process to respond to NO, a spontaneous byproduct of the anaerobic respiration. Importantly, the non-elongated NIR-deficient mutant failed to form biofilm, while a mutant of nitrate reductase (NAR) and wild type PAO1, both of which were highly elongated, formed robust biofilm. Taken together, our data reveal a role of previously undescribed cell biological event in P. aeruginosa biofilm formation and suggest NIR as a key player involved in such process.
Department of Microbiology, Yonsei University College of Medicine, Seoul, Republic of Korea.
Mi Young
MY
Yoon
Kang-Mu
KM
Lee
Yongjin
Y
Park
Sang Sun
SS
Yoon
D000693:Anaerobiosis; D018441:Biofilms; D050505:Mutant Proteins; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology; D009572:Nitrite Reductases / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000502:physiology*
D050505:Mutant Proteins; D009569:Nitric Oxide; D009572:Nitrite Reductases
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