Rhodamine hydrazone derivatives as Hg2+ selective fluorescent and colorimetric chemosensors and their applications to bioimaging and microfluidic system.
In this paper, we report new rhodamine hydrazone derivatives bearing thiol and carboxylic acid groups as selective fluorescent and colorimetric chemosensors for Hg(2+). The ring-opening process of spirolactam enables the large fluorescent enhancement and colorimetric change upon the addition of Hg(2+). The sample containing Hg(2+) was mixed with one of the chemosensors in a microchannel where the sensor was examined using confocal laser scanning microscopy. A plot of the fluorescent intensities of both chemosensors versus the log concentration of Hg(2+) exhibited a linear response (r(2)=0.95) in the range of 1 nM-1 μM, and the detection limits were 1 nM and 4.2 nM, respectively. Both chemosensors also enable the visualization of Hg(2+) accumulated in the nematode Caenorhabditis elegans previously exposed to nanomolar concentrations of Hg(2+).
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Ha Na
HN
Kim
Seong-Won
SW
Nam
K M K
KM
Swamy
Yan
Y
Jin
Xiaoqiang
X
Chen
Yonugmee
Y
Kim
Sung-Jin
SJ
Kim
Sungsu
S
Park
Juyoung
J
Yoon
D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008968:Molecular Conformation; D057054:Molecular Imaging / Q000379:methods*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
D005456:Fluorescent Dyes; D010456:Peptides, Cyclic; D012235:Rhodamines; C110394:chemosensor 1; C110395:chemosensor 2; D008628:Mercury