[Preparation of Ganoderma lucidum polysaccharides and triterpenes microemulsion and its anticancer effect in mice with transplant Heps tumors].
OBJECTIVE
To research the microemulsion preparation of Ganoderma lucidum polysaccharides and triterpenes and investigate its properities. Evaluate the effects of polysaccharides and triterpenes microemulsions against transplant tumor growth.


METHODS
The microemulsion formula was optimized by constructing the pseudo-ternary phase diagrams of blank microemulsion. The polysaccharides and triterpenes microemulsions were prepared on the blank microemulsions. The appearance, particle distribution and Zeta potential were investigated by transmission electron microscope and grain size analyzer. The Heps mice were randomly administered with polysaccharides and triterpenes microemulsions (114.5, 57.25 mg x kg(-1) x d(-1)) for 7 days. The effectiveness was assessed based on tumor inhibitory ratio of mice with Heps tumors. The toxicity was evaluated by measurements of the mice weight, immune organ weight.


RESULTS
The optimal microemulsion formula was composed of tween 20, dimethyl carbinol, water and 9-octadecenoic acid with the ratio of 14.3: 14.3: 33. 3:2. Polysaccharides and triterpenes microemulsions in transmission electron microscope were consisted of small spherical drop. The average particle size was 32.43 nm and the Zeta potential was -3.41 mV. The polysaccharides and triterpenes microemulsions showed an inhibition rate of 37.66% (57.25 mg x kg(-1) x d(-1)) and 52.34% (114.5 mg x kg(-1) x d(-1)) respectively against Heps tumor growth.


CONCLUSIONS
The acquired microemulsion with small particle size is stable. It significantly inhibits the tumor growth in Heps mice.
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Key Laboratory of Scientific Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. ychen202@hotmail.com
Yan
Y
Chen
Hui
H
Lu
Shihua
S
Song
Xiaobin
X
Jia
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D004655:Emulsions; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D010316:Particle Size; D011134:Polysaccharides / Q000627:therapeutic use*; D020940:Reishi / Q000737:chemistry*; D014315:Triterpenes / Q000627:therapeutic use*
D000972:Antineoplastic Agents, Phytogenic; D004655:Emulsions; D011134:Polysaccharides; D014315:Triterpenes