A modified electro-transformation method for Bacillus subtilis and its application in the production of antimicrobial lipopeptides.
A modified electroporation method using trehalose is presented for the transformation of Bacillus subtilis. The new method improved the transformation efficiency of B. subtilis nearly 2,000-fold compared with the usual method, giving 4×10(5) transformants/μg DNA. Using this method, B. subtilis was engineered to improve production of antimicrobial lipopeptides and produced 1.8-fold more surfactin and 2.9-fold more fengycin.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. caoguoqiang99@gmail.com
Guoqiang
G
Cao
Xiaohui
X
Zhang
Lei
L
Zhong
Zhaoxin
Z
Lu
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000235:genetics*; D053898:Biosynthetic Pathways / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D018274:Electroporation / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D055666:Lipopeptides / Q000096:biosynthesis*; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D055666:Lipopeptides