Molecular determinant-based typing of KIR alleles and KIR ligands.
Killer cell immunoglobulin-like receptors (KIRs) regulate NK cell function. KIRs and their HLA ligands are highly polymorphic in nature with substantial allelic polymorphism. At present, there is a lack of an expedient method for KIR and HLA allele typing with relevant functional information. Here, we developed a single-nucleotide polymorphism (SNP) assay to type various allele groups of KIR2DL1 with distinct functional properties based on polymorphism at position 245. We also established a SNP assay to type different KIR ligands based on polymorphism at position 77 in HLA-C and position 83 in HLA-B and -A. Our SNP assays for KIR and KIR ligand typing are much cheaper and faster than existing high-resolution typing. Importantly, our high-throughput methods provide readouts that are informative in predicting NK cell activity in health, disease, and transplantation.
Department of Oncology, St Jude Children's Research Hospital, Memphis, TN, USA.
Rafijul
R
Bari
Matthias
M
Leung
Victoria E
VE
Turner
Christy
C
Embrey
Barbara
B
Rooney
Martha
M
Holladay
Wing
W
Leung
D000483:Alleles; D002460:Cell Line; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology; D015235:HLA-B Antigens / Q000235:genetics / Q000276:immunology; D015236:HLA-C Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D008024:Ligands; D020641:Polymorphism, Single Nucleotide; D054342:Receptors, KIR2DL1 / Q000235:genetics* / Q000276:immunology*; D017422:Sequence Analysis, DNA / Q000379:methods*
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; C093100:KIR2DL1 protein, human; D008024:Ligands; D054342:Receptors, KIR2DL1
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