Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse.
Multiplex PCR has been developed for parallel identification of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis, important pathogens of swine, responsible for considerable economic losses in swine industry. Multiplex PCR and bacteriological cultivation was used to analyze lung samples from slaughterhouse pigs. From a total of 219 lung samples, 164 (74.9 %) were positive for P. multocida, 45 (20.5 %) for A. pleuropneumoniae and 4 (1.83 %) for H. parasuis. Bacteriological examination revealed that 145 samples (66.2 %) were positive for P. multocida, 31 (14.2 %) for A. pleuropneumoniae and 2 (0.91 %) for H. parasuis.
Centre for Analysis of DNA, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, 04181 Košice, Slovakia. Hricinova@uvmf.sk
M
M
Hričínová
E
E
Holoda
D
D
Mudroňová
S
S
Ondrašovičová
D000003:Abattoirs; D016977:Actinobacillus pleuropneumoniae / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers; D044137:Haemophilus parasuis / Q000235:genetics / Q000302:isolation & purification*; D008168:Lung / Q000382:microbiology; D016979:Pasteurella multocida / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*
D017931:DNA Primers
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