Persistence of free-living protozoan communities across rearing cycles in commercial poultry houses.
The introduction and survival of zoonotic bacterial pathogens in poultry farming have been linked to bacterial association with free-living protozoa. To date, however, no information is available on the persistence of protozoan communities in these environments across consecutive rearing cycles and how it is affected by farm- and habitat-specific characteristics and management strategies. We therefore investigated the spatial and temporal dynamics of free-living protozoa in three habitats (pipeline, water, and miscellaneous samples) in three commercial poultry houses across three rearing cycles by using the molecular fingerprinting technique denaturing gradient gel electrophoresis (DGGE). Our study provides strong evidence for the long-term (ca. 6-month) persistence of protozoa in broiler houses across consecutive rearing cycles. Various free-living protozoa (flagellates, ciliates, and amoebae), including known vectors of bacterial pathogens, were observed during the down periods in between rearing cycles. In addition, multivariate analysis and variation partitioning showed that the protozoan community structure in the broiler houses showed almost no change across rearing cycles and remained highly habitat and farm specific. Unlike in natural environments, protozoan communities inside broiler houses are therefore not seasonal. Our results imply that currently used biosecurity measures (cleaning and disinfection) applied during the down periods are not effective against many protozoans and therefore cannot prevent potential cross-contamination of bacterial pathogens via free-living protozoa between rearing cycles.
Department of Biology, Section Protistology and Aquatic Ecology, Faculty of Sciences, Ghent University, Krijgslaan 281-S8, 9000 Ghent, Belgium.
Julie
J
Baré
Kurt
K
Houf
Tine
T
Verstraete
Mario
M
Vaerewijck
Koen
K
Sabbe
D056893:Alveolata / Q000302:isolation & purification*; D000818:Animals; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004783:Environmental Microbiology; D006799:Housing, Animal; D050296:Microbial Viability; D009691:Nucleic Acid Denaturation; D011200:Poultry / Q000469:parasitology*; D013997:Time Factors
D016054:DNA, Protozoan
Appl Environ Microbiol. 2009 Mar;75(5):1417-26
19124593
Eur J Protistol. 2009 May;45(2):121-38
19285382
Appl Environ Microbiol. 2004 Jun;70(6):3706-14
15184177
Ecology. 2007 Aug;88(8):1924-31
17824422
FEMS Microbiol Rev. 2010 May;34(3):231-59
19744244
Int J Food Microbiol. 2008 Jul 15;125(2):182-7
18508146
Proc Biol Sci. 2005 Oct 22;272(1577):2203-10
16191631
Parasitol Res. 2007 Sep;101(4):1097-102
17579885
FEMS Microbiol Ecol. 2010 Nov;74(2):371-81
20722733
Appl Environ Microbiol. 1988 Dec;54(12):3023-33
3223766
Br J Ophthalmol. 2008 Jan;92(1):112-5
17965106
Appl Environ Microbiol. 2003 Aug;69(8):4343-51
12902214
FEMS Microbiol Lett. 2008 May;282(2):258-65
18399997
Protist. 2000 May;151(1):69-80
10896134
J Mol Biol. 1990 Oct 5;215(3):403-10
2231712
Appl Environ Microbiol. 2009 Jul;75(14):4736-46
19465529
Exp Parasitol. 2010 Sep;126(1):59-64
20056117
Appl Environ Microbiol. 2005 Sep;71(9):5560-71
16151149
Microbes Infect. 2006 Feb;8(2):578-87
16298155
Appl Environ Microbiol. 2005 Feb;71(2):987-92
15691957
FEMS Microbiol Ecol. 2009 Oct;70(1):20-9
19645820
Avian Pathol. 2000 Apr;29(2):123-31
19184798
J Phycol. 2008 Feb;44(1):164-72
27041053
Cell Microbiol. 2004 Dec;6(12):1127-38
15527493
Protist. 2009 May;160(2):159-89
19324594
Microbiology (Reading). 2007 Jun;153(Pt 6):1781-1789
17526835
Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10644-9
15247413
FEBS Lett. 2007 Jun 19;581(15):2829-38
17531986
Vet Microbiol. 2007 Jul 20;123(1-3):104-9
17462837
FEMS Immunol Med Microbiol. 2007 Jun;50(1):1-26
17428307
Microb Ecol. 2003 Oct;46(3):358-69
14502413
J Eukaryot Microbiol. 2005 Sep-Oct;52(5):399-451
16248873
FEMS Microbiol Rev. 1997 Nov;21(3):213-29
9451814
Appl Environ Microbiol. 2005 Mar;71(3):1562-9
15746361
Infect Immun. 1997 Sep;65(9):3759-67
9284149