Detection of allergenic compounds using an IL-4/luciferase/CNS-1 transgenic mice model.
The interleukin-4 (IL-4) signaling cascade has been identified as a potentially important pathway in the development of allergies. The principal objective of this study was to produce novel transgenic (Tg) mice harboring the luciferase gene under the control of the human IL-4 promoter and the enhancer of IL-4 (CNS-1), in an effort to evaluate three types of allergens including a respiratory sensitizer, vaccine additives, and crude extracts of natural allergens in vivo. A new lineage of Tg mice was generated by the microinjection of pIL-4/Luc/CNS-1 constructs into a fertilized mice egg. The luciferase activity was successfully regulated by the IL-4 promoter in splenocytes cultured from IL-4/Luc/CNS-1 Tg mice. From the first five founder lines, one (#57) evidencing a profound response to ovalbumin was selected for use in evaluating the allergens. Additionally, the lungs, thymus, and lymph nodes of IL-4/Luc/CNS-1 Tg mice evidenced high luciferase activity in response to allergens such as phthalic anhydride (PA), trimellitic anhydride, ovalbumin, gelatin, Dermatophagoides pteronyssinus extracts, and Japanese cedar pollen, whereas key allergy-related indicators including ear thickness, Immunoglobulin E concentration, and the infiltration of inflammatory leukocytes in response to PA were unaltered in the Tg mice relative to the non-Tg mice. Furthermore, the expression levels of endogenous type 2 helper T cells cytokines and proinflammatory cytokines were similarly increased in these organs of IL-4/Luc/CNS-1 Tg mice in response to allergens. These results indicate that IL-4/Luc/CNS-1 Tg mice may be used as an animal model for the evaluation and prediction of the human body response to a variety of allergens originating from the environment and from certain industrial products.
Laboratory Animal Resources Division, Korea Food and Drug Administration, Seoul 122-704, Korea.
Chang Joon
CJ
Bae
Jae Won
JW
Lee
Hee Sook
HS
Bae
Sun Bo
SB
Shim
Seung Wan
SW
Jee
Su Hae
SH
Lee
Chang Kyu
CK
Lee
Jin Tae
JT
Hong
Dae Youn
DY
Hwang
D000485:Allergens / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D049409:Luciferases, Firefly / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D019076:Transgenes / Q000235:genetics / Q000276:immunology*
D000485:Allergens; C508598:IL4 protein, human; D015847:Interleukin-4; D049409:Luciferases, Firefly