Effect of diabetes on orthodontic tooth movement in a mouse model.
Orthodontic tooth movement is achieved by the remodeling of alveolar bone in response to mechanical loading. Type 1 diabetes results in bone remodeling, suggesting that this disease might affect orthodontic tooth movement. The present study investigated the effects of the diabetic state on orthodontic tooth movement. An orthodontic appliance was placed in normoglycemic (NG), streptozotocin-induced diabetes (DB), and insulin-treated DB (IT) C57BL6/J mice. Histomorphometric analysis and quantitative PCR of periodontium were performed. The DB mice exhibited greater orthodontic tooth movement and had a higher number of tartrate-resistant acid phosphate (TRAP) -positive osteoclasts than NG mice. This was associated with increased expression of factors involved in osteoclast activity and recruitment (Rankl, Csf1, Ccl2, Ccl5, and Tnfa) in DB mice. The expression of osteoblastic markers (Runx2, Ocn, Col1, and Alp) was decreased in DB mice. Reversal of the diabetic state by insulin treatment resulted in morphological findings similar to those of NG mice. These results suggest that the diabetic state up-regulates osteoclast migration and activity and down-regulates osteoblast differentiation, resulting in greater orthodontic tooth movement.
Department of Orthodontics, Faculty of Dentistry, Pontifícia Universidade Católica de Minas Gerais (PUC-Minas), Belo Horizonte, Minas Gerais, Brazil.
Sarah M G
SM
Braga
Silvana R de Albuquerque
SR
Taddei
Ildeu
I
Andrade
Celso M
CM
Queiroz-Junior
Gustavo P
GP
Garlet
Carlos E
CE
Repeke
Mauro M
MM
Teixeira
Tarcília A
TA
da Silva
D000135:Acid Phosphatase / Q000378:metabolism; D000539:Alveolar Process / Q000378:metabolism; D000818:Animals; D016723:Bone Remodeling; D002454:Cell Differentiation; D002465:Cell Movement; D018925:Chemokines / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D004195:Disease Models, Animal; D007328:Insulin / Q000627:therapeutic use; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000502:physiology; D010010:Osteoclasts / Q000502:physiology; D010513:Periodontal Ligament / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase; D014087:Tooth Movement Techniques
D018925:Chemokines; D016207:Cytokines; D007328:Insulin; D007527:Isoenzymes; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase