Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects.
We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2 μm depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z.
Department of Electrical Engineering, Stanford University, 350 Serra Mall, Stanford, California 94305, USA.
Matthew D
MD
Lew
Steven F
SF
Lee
Majid
M
Badieirostami
W E
WE
Moerner
D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008853:Microscopy; D036103:Nanotechnology / Q000379:methods*; D055620:Optical Phenomena
D004129:Dimethylpolysiloxanes; C013830:baysilon
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