Mitochondrial proteomics analysis of tumorigenic and metastatic breast cancer markers.
Mitochondria are key organelles in mammary cells responsible for several cellular functions including growth, division, and energy metabolism. In this study, mitochondrial proteins were enriched for proteomics analysis with the state-of-the-art two-dimensional differential gel electrophoresis and matrix-assistant laser desorption ionization-time-of-flight mass spectrometry strategy to compare and identify the mitochondrial protein profiling changes between three breast cell lines with different tumorigenicity and metastasis. The proteomics results demonstrate more than 1,500 protein features were resolved from the equal amount pooled from three purified mitochondrial proteins, and 125 differentially expressed spots were identified by their peptide finger print, in which, 33 identified proteins belonged to mitochondrial proteins. Eighteen out of these 33 identified mitochondrial proteins such as SCaMC-1 have not been reported in breast cancer research to our knowledge. Additionally, mitochondrial protein prohibitin has shown to be differentially distributed in mitochondria and in nucleus for normal breast cells and breast cancer cell lines, respectively. To sum up, our approach to identify the mitochondrial proteins in various stages of breast cancer progression and the identified proteins may be further evaluated as potential breast cancer markers in prognosis and therapy.
Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, No. 101, Kuang-Fu Rd. Sec. 2, Hsinchu, 30013, Taiwan.
Yi-Wen
YW
Chen
Hsiu-Chuan
HC
Chou
Ping-Chiang
PC
Lyu
Hsien-Sheng
HS
Yin
Fang-Liang
FL
Huang
Wun-Shaing Wayne
WS
Chang
Chiao-Yuan
CY
Fan
I-Fan
IF
Tu
Tzu-Chia
TC
Lai
Szu-Ting
ST
Lin
Ying-Chieh
YC
Lu
Chieh-Lin
CL
Wu
Shun-Hong
SH
Huang
Hong-Lin
HL
Chan
D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D000091402:Prohibitins; D040901:Proteomics; D012097:Repressor Proteins / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
D014408:Biomarkers, Tumor; D024101:Mitochondrial Proteins; D000091402:Prohibitins; D012097:Repressor Proteins
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