[Clone and construction of plant expression vector of AeSS gene in Aralia elata].
OBJECTIVE
To clone Aralia elata squalene synthase gene (designated as AeSS) and construct plant expression vector for transgenic research.


METHODS
Isolated squalene synthase from Aralia elata with specific primers by RT-PCR and inserted AeSS gene into the plant expression vector pBI121.


RESULTS
The full-length cDNA of AeSS (Genebank accession Number: GU354313) was 1 261 bp and contained a 1 245 bp open reading frame (ORF) encoding a polypeptide of 414 amino acids. The plant expression vector pAeSS was constructed by inserted AeSS gene into the downstream of 35 S promoter of plant expression vector pBI121.


CONCLUSIONS
AeSS gene was cloned and plant expression vector was constructed for future research.
College of Plant Science, Jilin University, Changchun 130062, China. wuying35969@126.com
Ying
Y
Wu
Hui
H
Cheng
Hong-Da
HD
Zou
Dong-Mei
DM
Jia
Chun-Yan
CY
Zhao
Ya-Ping
YP
Yuan
D000595:Amino Acid Sequence; D027983:Aralia / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D010940:Plant Proteins; D013186:Farnesyl-Diphosphate Farnesyltransferase