1H-MRS in spinal cord injury: acute and chronic metabolite alterations in rat brain and lumbar spinal cord.
A variety of tests of sensorimotor function are used to characterize outcome after experimental spinal cord injury (SCI). These tests typically do not provide information about chemical and metabolic processes in the injured CNS. Here, we used (1) H-magnetic resonance spectroscopy (MRS) to monitor long-term and short-term chemical changes in the CNS in vivo following SCI. The investigated areas were cortex, thalamus/striatum and the spinal cord distal to injury. In cortex, glutamate (Glu) decreased 1 day after SCI and slowly returned towards normal levels. The combined glutamine (Gln) and Glu signal was similarly decreased in cortex, but increased in the distal spinal cord, suggesting opposite changes of the Glu/Gln metabolites in cortex and distal spinal cord. In lumbar spinal cord, a marked increase of myo-inositol was found 3 days, 14 days and 4 months after SCI. Changes in metabolite concentrations in the spinal cord were also found for choline and N-acetylaspartate. No significant changes in metabolite concentrations were found in thalamus/striatum. Multivariate data analysis allowed separation between rats with SCI and controls for spectra acquired in cortex and spinal cord, but not in thalamus/striatum. Our findings suggest MRS could become a helpful tool to monitor spatial and temporal alterations of metabolic conditions in vivo in the brain and spinal cord after SCI. We provide evidence for dynamic temporal changes at both ends of the neuraxis, cortex cerebri and distal spinal cord, while deep brain areas appear less affected.
Department of Neuroscience, Karolinska Institutet, Retzius väg 8, SE-171 77 Stockholm, Sweden.
Matthias
M
Erschbamer
Johanna
J
Oberg
Eric
E
Westman
Rouslan
R
Sitnikov
Lars
L
Olson
Christian
C
Spenger
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology*
D005973:Glutamine; D018698:Glutamic Acid
J Neurotrauma. 2010 Mar;27(3):599-610
20001674
Invest Radiol. 1997 Jul;32(7):382-8
9228603
Nat Rev Neurosci. 2008 Jun;9(6):481-93
18490917
Prog Neurobiol. 1995 Aug;46(5):531-40
8532851
J Neurosci. 2004 Mar 3;24(9):2143-55
14999065
Eur J Neurosci. 2003 Dec;18(11):3061-8
14656301
MAGMA. 2007 Oct;20(4):183-91
17876622
Nat Rev Neurosci. 2006 Aug;7(8):644-53
16858392
J Neurosci Res. 2004 Dec 1;78(5):749-59
15499591
J Neurosci. 1997 Feb 1;17(3):1055-63
8994060
Magn Reson Med. 2004 Jun;51(6):1122-8
15170831
Magn Reson Med. 2001 Dec;46(6):1216-22
11746589
Nat Med. 1997 Dec;3(12):1329-35
9396602
Neuroscience. 1999;93(4):1383-9
10501463
NeuroRx. 2005 Apr;2(2):197-214
15897945
Brain Res. 1989 Jun 19;490(1):144-7
2758321
NMR Biomed. 2006 Oct;19(6):655-68
16986115
Magn Reson Med. 1996 Apr;35(4):443-8
8992192
J Neurochem. 1996 Feb;66(2):717-22
8592144
AJNR Am J Neuroradiol. 2002 Jun-Jul;23(6):901-5
12063213
Ann Neurol. 1988 Jun;23(6):623-6
2841902
Brain Res Bull. 1999 Aug;49(6):377-91
10483914
Dev Neurosci. 1993;15(3-5):289-98
7805581
NMR Biomed. 2001 Jun;14(4):260-4
11410943
Magn Reson Med. 2008 Jun;59(6):1250-8
18421679
J Neurotrauma. 2003 Aug;20(8):725-43
12965052
Curr Biol. 2001 Nov 13;11(22):1766-70
11719218
J Histochem Cytochem. 1993 Apr;41(4):559-70
8450195
Prog Neurobiol. 2007 Feb;81(2):89-131
17275978
Radiology. 1990 Aug;176(2):509-15
2164237
J Neurosci. 1994 Nov;14(11 Pt 1):6598-607
7965063
Nat Rev Neurosci. 2004 Feb;5(2):146-56
14735117
J Neurol Neurosurg Psychiatry. 1998 Nov;65(5):748-54
9810950
J Neurotrauma. 1995 Feb;12(1):1-21
7783230
Magn Reson Med. 1999 Apr;41(4):649-56
10332839
J Comp Neurol. 1997 Jan 20;377(3):443-64
8989657
J Neurosurg. 1997 Mar;86(3):483-92
9046306
Childs Nerv Syst. 1992 Dec;8(8):468-70
1288858
Magn Reson Med. 1993 Dec;30(6):672-9
8139448
Neuroradiology. 2000 Jul;42(7):515-7
10952184
Nat Med. 2005 May;11(5):572-7
15821747
J Neurochem. 2006 Feb;96(3):861-9
16371008
Acta Physiol Scand Suppl. 1980;492:91-119
6939309
Magn Reson Med. 2003 Jul;50(1):24-32
12815675
J Neurosurg. 1991 Jul;75(1):15-26
2045903
Prog Brain Res. 2006;157:231-260
17167915
J Neurotrauma. 2006 Oct;23(10):1427-35
17020480
Exp Neurol. 2007 Mar;204(1):58-65
17112518
Brain. 2000 Oct;123 ( Pt 10):2046-54
11004122
Xenobiotica. 1999 Nov;29(11):1181-9
10598751
J Neurotrauma. 2004 Jun;21(6):754-74
15253803
Magn Reson Med. 2008 Mar;59(3):499-506
18219631
Brain. 2007 Nov;130(Pt 11):2951-61
17913768
Philos Trans R Soc Lond B Biol Sci. 2006 Sep 29;361(1473):1611-34
16939978
J Neurotrauma. 2006 Mar-Apr;23(3-4):264-80
16629615
Ann Neurol. 1989 Jan;25(1):26-31
2913925
Exp Neurol. 1998 Oct;153(2):299-312
9784289