Activity and viability of polycyclic aromatic hydrocarbon-degrading Sphingomonas sp. LB126 in a DC-electrical field typical for electrobioremediation measures.
There has been growing interest in employing electro-bioremediation, a hybrid technology of bioremediation and electrokinetics for the treatment of contaminated soil. Knowledge however on the effect of weak electrokinetic conditions on the activity and viability of pollutant-degrading microorganisms is scarce. Here we present data about the influence of direct current (DC) on the membrane integrity, adenosine triphosphate (ATP) pools, physico-chemical cell surface properties, degradation kinetics and culturability of fluorene-degrading Sphingomonas sp. LB126. Flow cytometry was applied to quantify the uptake of propidium iodide (PI) and the membrane potential-related fluorescence intensities (MPRFI) of individual cells within a population. Adenosine tri-phosphate contents and fluorene biodegradation rates of bulk cultures were determined and expressed on a per cell basis. The cells' surface hydrophobicity and electric charge were assessed by contact angle and zeta potential measurements respectively. Relative to the control, DC-exposed cells exhibited up to 60% elevated intracellular ATP levels and yet remained unaffected on all other levels of cellular integrity and functionality tested. Our data suggest that direct current (X=1 V cm(-1); J=10.2 mA cm(-2)) as typically used for electrobioremediation measures has no negative effect on the activity of the polycyclic aromatic hydrocarbon (PAH)-degrading soil microorganism, thereby filling a serious gap of the current knowledge of the electrobioremediation methodology.
UFZ Helmholtz-Center for Environmental Research, Department of Environmental Microbiology, 04318 Leipzig, Germany.
Lei
L
Shi
Susann
S
Müller
Norbert
N
Loffhagen
Hauke
H
Harms
Lukas Y
LY
Wick
D000255:Adenosine Triphosphate / Q000378:metabolism; D001673:Biodegradation, Environmental; D004560:Electricity; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism*; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism; D020578:Sphingomonas / Q000737:chemistry* / Q000378:metabolism*; D013499:Surface Properties
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D000255:Adenosine Triphosphate
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