Considerations for potency equivalent calculations in the Ah receptor-based CALUX bioassay: normalization of superinduction results for improved sample potency estimation.
The chemically activated luciferase expression (CALUX) system is a mechanistically based recombinant luciferase reporter gene cell bioassay used in combination with chemical extraction and clean-up methods for the detection and relative quantitation of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like halogenated aromatic hydrocarbons in a wide variety of sample matrices. While sample extracts containing complex mixtures of chemicals can produce a variety of distinct concentration-dependent luciferase induction responses in CALUX cells, these effects are produced through a common mechanism of action (i.e. the Ah receptor (AhR)) allowing normalization of results and sample potency determination. Here we describe the diversity in CALUX response to PCDD/Fs from sediment and soil extracts and not only report the occurrence of superinduction of the CALUX bioassay, but we describe a mechanistically based approach for normalization of superinduction data that results in a more accurate estimation of the relative potency of such sample extracts.
Department of Environmental Toxicology, University of California, Davis, CA 95616-8588, USA.
David S
DS
Baston
Michael S
MS
Denison
D000818:Animals; D001681:Biological Assay / Q000379:methods; D045744:Cell Line, Tumor; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics; D051379:Mice; D018336:Receptors, Aryl Hydrocarbon / Q000737:chemistry*; D012987:Soil / Q000737:chemistry
D018336:Receptors, Aryl Hydrocarbon; D012987:Soil; D008156:Luciferases
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