Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts.
CXC chemokine ligand 10 (CXCL10) plays an important role in the infiltration of Th1 cells and thus in the exacerbation of periodontal disease. Theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, has some beneficial effects but the effect of TFDG on CXCL10 production from human gingival fibroblasts (HGFs) is uncertain. In this study, we investigated the mechanisms by which TFDG may inhibit oncostatin M (OSM)-induced CXCL10 production in human gingival fibroblasts. TFDG prevented OSM-mediated CXCL10 production by HGFs in a dose dependent manner. TFDG significantly inhibited OSM-induced phosphorylation of c-Jun N terminal kinase (JNK), protein kinase B (Akt) (Ser473) that are related to CXCL10 production from OSM-stimulated HGFs. In addition, TFDG suppressed OSM receptor (OSMR) β expression on HGFs. These data provide a novel mechanism where the black tea flavonoid, theaflavin, could provide direct benefits in periodontal disease.
Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Japan. hosokawa@dent.tokushima-u.ac.jp
Yoshitaka
Y
Hosokawa
Ikuko
I
Hosokawa
Satoru
S
Shindo
Kazumi
K
Ozaki
Tadashi
T
Nakanishi
Hideaki
H
Nakae
Takashi
T
Matsuo
D044946:Biflavonoids / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005419:Flavonoids / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005881:Gingiva / Q000473:pathology*; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D053683:Oncostatin M / Q000276:immunology / Q000378:metabolism; D053681:Oncostatin M Receptor beta Subunit / Q000235:genetics / Q000378:metabolism; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000276:immunology; D010636:Phenols / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D059808:Polyphenols; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D013662:Tea
D044946:Biflavonoids; D054357:Chemokine CXCL10; D005419:Flavonoids; D053681:Oncostatin M Receptor beta Subunit; D010636:Phenols; D059808:Polyphenols; D050796:STAT3 Transcription Factor; D013662:Tea; D053683:Oncostatin M; C077197:theaflavin digallate; D005707:Gallic Acid; D002392:Catechin; D051057:Proto-Oncogene Proteins c-akt; D048670:MAP Kinase Kinase 4