Catalytic characteristics of NADP-isocitrate dehydrogenase from the liver of rats in health and after injection of TNF-α and melatonin treatment.
Activity of NADP-dependent isocitrate dehydrogenase (EC 1.1.1.42) increases and the catalytic characteristics of the enzyme isolated from the liver of rats are changed under conditions of apoptosis induction in comparison with these characteristics in health. Injection of melatonin induced a trend to normalization of kinetic parameters of catalytic activity and of some regulatory characteristics of the enzyme.
Voronezh State University, Russia. tsvn@front.ru
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Rakhmanova
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002384:Catalysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003609:Dactinomycin / Q000008:administration & dosage / Q000494:pharmacology; D004586:Electrophoresis; D007274:Injections, Intraperitoneal; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008550:Melatonin / Q000494:pharmacology*; D051381:Rats; D013053:Spectrophotometry; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D008550:Melatonin