Heparinized polyvinyl alcohol to specifically adsorb low-density lipoprotein from plasma.
BACKGROUND
A medical adsorbent for blood purification was developed to specifically adsorb low-density lipoprotein (LDL) from hypercholesterolemia patient's plasma by covalently immobilizing heparin onto the surface of polyvinyl alcohol (PVA) with the couplant toluence-2,4-diisocyanate (TDI).


METHODS
We used IR to demonstrate the success of covalently immobilizing heparin onto the surface, and investigated its adsorption of LDL, and primarily evaluated its hemo-compatibility using tests for platelet adhesion, the degree of platelet activation and a hemolysis test.


RESULTS
(1) Heparin was successfully covalently immobilized onto the surface, the maximum amount of heparin immobilized on the surface of 1g PVA-1799 granules was about 5 μg; (2) one optimal condition for adsorption of LDL from hyperlipidemia plasma was a pH within the range of 7.2∼9.5, accordingly the adsorptive ratio (adsorbent/g: plasma/L=1:2) for LDL was about 70%; (3) it exhibited good hemo-compatibility.


CONCLUSIONS
The adsorbent results in satisfactory adsorption of LDL with good hemo-compatibility; it could potentially be used as a blood purification material, and immobilization of heparin onto medical materials may be a way to develop an LDL-specific adsorbent for blood purification.
College of Medical Technology & Engineering, Henan University of Science & Technology, Luoyang 471003, PR China. mkw007@126.com
Kai-wang
KW
Ma
Xiao-zhen
XZ
Dai
Shu-ying
SY
Feng
Ai-hua
AH
Jing
Jian-ying
JY
Yang
D000327:Adsorption; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects / Q000648:ultrastructure; D004355:Drug Stability; D006461:Hemolysis; D006493:Heparin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000628:therapy; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019007:P-Selectin / Q000032:analysis; D010956:Plasmapheresis / Q000295:instrumentation / Q000379:methods*; D015539:Platelet Activation / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D013055:Spectrophotometry, Infrared
D008077:Lipoproteins, LDL; D019007:P-Selectin; D011142:Polyvinyl Alcohol; D006493:Heparin