Evaluation of ELISA and IHAT in serological diagnosis of proven cases of human hydatidosis.
Cystic hydatid disease (Hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. Hydatidosis in man (as an accidental host) is caused by infection with the ova containing larval stage of Echinococcus granulosus. In the last decade, different techniques have been employed for sero-diagnosis of hydatidosis; as IHA, IFA, ELISA, CCLE (Counter Current Immuno-electrophoresis). This paper evaluated the validity of ELISA and IHA. Since whole hydatid cyst fluid was used as a source of antigen for serodiagnosis. Thirty surgical and pathological hydatidosis proven patients were examined. The sensitivity and specificity of ELISA were 96.7% & 97.5% respectively, and that of IHA were 86.7%, &95% respectively.
Department of Medical Parasitology, Faculty of Medicine, University of Mansoura, Saudi Arabia.
A M
AM
El-Shazly
Ranya M
RM
Saad
Usama S
US
Belal
Tarek
T
Sakr
Hytham A
HA
Zakae
D000328:Adult; D000913:Antibodies, Protozoan; D004443:Echinococcosis / Q000097:blood* / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D008297:Male; D012698:Serologic Tests / Q000379:methods*
D000913:Antibodies, Protozoan