Development of novel intracytoplasmic sperm injection and somatic cell nuclear transfer techniques for animal reproduction.
Animal biotechnology has made new biological experiments possible and new discoveries are being made on an almost daily basis. Among these discoveries is a method for directly injecting a spermatozoa or somatic nucleus into an oocyte that has brought a revolution in the world of micromanipulators. Experiments that were unfeasible until now have become possible, and normal offspring can be generated from infertile cells, such as using dead sperm or a dead frozen body. In this review, I will introduce the progress of animal reproductive biotechnology, including our current research.
RIKEN Center for Developmental Biology, Chuo, Kobe, Japan. teru@cdb.riken.jp
Teruhiko
T
Wakayama
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D006224:Cricetinae; D051379:Mice; D008846:Micromanipulation / Q000379:methods*; D053652:Nuclear Transfer Techniques; D012099:Reproductive Techniques; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*