Multifunctional silica nanotubes for dual-modality gene delivery and MR imaging.
This work demonstrated the development of multifunctional silica nanotubes (SNT) by functionalization of their inner void and outer surface with magnetic-fluorescence nanocomposites and cationic polymers, respectively. The successful construction of BPEI-SNT was established by electron energy loss spectroscopy in conjugation with standard analytical tools. The mean fluorescence intensity in a FACS assay, a luciferase gene expression assay and a confocal fluorescence study demonstrated the efficacy of BPEI-SNT as a gene delivery vector. Endocytotic uptake was also demonstrated by the colocalization of LysoTracker Red(®) and green fluorescent quantum dots. Moreover, enhanced magnetic resonance imaging revealed the potential of the BPEI-SNT nanocomposite to act as a dual-modality nano-device.
Department of Chemistry, BK21 program, Polymer Research Institute, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, Republic of Korea.
Ran
R
Namgung
Yuhai
Y
Zhang
Qi Ling
QL
Fang
Kaushik
K
Singha
Hwa Jeong
HJ
Lee
Il Keun
IK
Kwon
Yong Yeon
YY
Jeong
In-Kyu
IK
Park
Sang Jun
SJ
Son
Won Jong
WJ
Kim
D003952:Diagnostic Imaging / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000737:chemistry; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry*
D005290:Ferric Compounds; C000499:ferric oxide; D012822:Silicon Dioxide; D011094:Polyethyleneimine