The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice.
Submergence and drought are major constraints to rice (Oryza sativa) production in rain-fed farmlands, both of which can occur sequentially during a single crop cycle. SUB1A, an ERF transcription factor found in limited rice accessions, dampens ethylene production and gibberellic acid responsiveness during submergence, economizing carbohydrate reserves and significantly prolonging endurance. Here, we evaluated the functional role of SUB1A in acclimation to dehydration. Comparative analysis of genotypes with and without SUB1A revealed that SUB1A enhanced recovery from drought at the vegetative stage through reduction of leaf water loss and lipid peroxidation and increased expression of genes associated with acclimation to dehydration. Overexpression of SUB1A augmented ABA responsiveness, thereby activating stress-inducible gene expression. Paradoxically, vegetative tissue undergoes dehydration upon desubmergence even though the soil contains sufficient water, indicating that leaf desiccation occurs in the natural progression of a flooding event. Desubmergence caused the upregulation of gene transcripts associated with acclimation to dehydration, with higher induction in SUB1A genotypes. SUB1A also restrained accumulation of reactive oxygen species (ROS) in aerial tissue during drought and desubmergence. Consistently, SUB1A increased the abundance of transcripts encoding ROS scavenging enzymes, resulting in enhanced tolerance to oxidative stress. Therefore, in addition to providing robust submergence tolerance, SUB1A improves survival of rapid dehydration following desubmergence and water deficit during drought.
Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, California 92521, USA.
Takeshi
T
Fukao
Elaine
E
Yeung
Julia
J
Bailey-Serres
D000040:Abscisic Acid / Q000494:pharmacology; D000064:Acclimatization; D003681:Dehydration; D055864:Droughts; D055868:Floods; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D015227:Lipid Peroxidation; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D018384:Oxidative Stress; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D017382:Reactive Oxygen Species / Q000032:analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000502:physiology
D010940:Plant Proteins; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D014867:Water; D000040:Abscisic Acid
Plant J. 2006 Jun;46(5):805-22
16709196
Plant Physiol. 2010 Mar;152(3):1484-500
20097791
Planta. 2017 Jun;245(6):1067
28456836
Science. 2006 Jan 6;311(5757):91-4
16400150
Plant Cell. 2005 Jan;17(1):268-81
15608336
Am Nat. 2009 Jan;173(1):113-8
19061420
Plant Mol Biol. 2010 Mar;72(4-5):557-66
20039193
Methods. 2001 Dec;25(4):402-8
11846609
Plant Physiol. 1999 Jan;119(1):57-64
9880346
Stat Appl Genet Mol Biol. 2004;3:Article3
16646809
Plant Physiol. 2010 Mar;152(3):1674-92
20107022
Plant J. 2007 Aug;51(4):617-30
17587305
Nature. 2006 Aug 10;442(7103):705-8
16900200
J Plant Physiol. 2005 Apr;162(4):465-72
15900889
Biotechnol Lett. 2008 Dec;30(12):2191-8
18779926
Annu Rev Plant Biol. 2008;59:313-39
18444902
J Exp Bot. 2007;58(12):3449-62
17898421
Genetics. 2006 Feb;172(2):1213-28
16272419
J Biol Chem. 2008 Dec 5;283(49):34197-203
18852264
Planta. 2000 Jan;210(2):279-85
10664134
Science. 1998 Oct 9;282(5387):287-90
9765153
Ann Bot. 2009 Jan;103(2):151-60
18974101
Plant Physiol. 2009 Jul;150(3):1368-79
19429605
Planta. 2006 Jul;224(2):300-14
16397796
FEBS Lett. 2006 Dec 11;580(28-29):6537-42
17112521
Plant Cell. 2006 Aug;18(8):2021-34
16816135
Annu Rev Plant Biol. 2007;58:183-98
17472566
Mol Genet Genomics. 2010 Feb;283(2):185-96
20049613
Plant Physiol. 2005 Oct;139(2):847-56
16183833
Curr Opin Plant Biol. 2007 Jun;10(3):296-302
17468040
Plant Physiol. 2008 Dec;148(4):1953-63
18945933
Ann Bot. 2009 Jan;103(2):181-96
18660495
Nat Protoc. 2008;3(6):1101-8
18546601
Plant J. 2001 Aug;27(4):325-33
11532178
Curr Biol. 2008 May 6;18(9):656-60
18450450
Plant Cell Rep. 2007 Oct;26(10):1909-17
17571267
Plant Mol Biol. 2009 Mar;69(4):451-62
19031047
Plant Physiol. 1992 Jul;99(3):1156-61
16668983
Plant Cell Physiol. 2006 Jan;47(1):141-53
16284406
Physiol Plant. 2004 Jun;121(2):231-238
15153190
Plant Physiol. 2009 Jan;149(1):88-95
19126699
Plant Physiol. 2010 Oct;154(2):969-77
20699400
Plant J. 2003 Feb;33(4):751-63
12609047
Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16814-9
18936491
Annu Rev Plant Biol. 2002;53:247-73
12221975
Plant Cell. 1990 Jan;2(1):19-27
2152105
Plant Physiol. 1991 Oct;97(2):593-7
16668440
Theor Appl Genet. 2007 Jun;115(1):35-46
17426956
Curr Opin Plant Biol. 2006 Apr;9(2):189-95
16483835
Biosci Biotechnol Biochem. 1999 Feb;63(2):302-8
10192910
Plant J. 2010 Mar;61(6):1041-52
20409277
Plant Physiol Biochem. 2010 May;48(5):359-73
20303775
Plant Physiol. 1987 Dec;85(4):1016-20
16665795
Plant Physiol. 1996 Jun;111(2):381-91
8787023
Ann Bot. 2010 Jan;105(1):129-39
19880423
Plant Physiol. 2004 May;135(1):384-99
15122029
Nature. 2009 Aug 20;460(7258):1026-30
19693083
Theor Appl Genet. 2010 Nov;121(8):1441-53
20652530
Trends Plant Sci. 2004 Oct;9(10):490-8
15465684
Plant Physiol. 2002 Feb;128(2):544-51
11842158
Plant Cell Environ. 2010 Apr;33(4):453-67
19712065
Theor Appl Genet. 2009 Dec;120(1):177-90
19841886
Plant Physiol. 2007 Apr;143(4):1467-83
17293439
Planta. 2006 Feb;223(3):604-12
16160845