[Optimization of extraction process of total amino acid from Bubali Cornu by orthogonal test].
OBJECTIVE
To optimize the extraction conditions of the total amino acid from Bubali Cornu.


METHODS
An orthogonal test of L9 (3(4)) was designed to select optimum exaction conditions of the total amino acid. The influence of solvent concentration, the dosage of solvent and time of extraction were investigated with the content of total amino acid and thrombin-induced fibrin clotting time as index.


RESULTS
The optimum extraction condition procedure was described as follows: the concentration of the solvent was 4 mol x L(-1), volume of solvent was 6 times amount of the materials.


CONCLUSIONS
The optimum exaction conditions procedure is reasonable and stable.
Kanion Pharmaceutical Co., Ltd, Lianyungang 222001, China.
Qiang
Q
Shang
Yan
Y
Liu
Jianxiong
J
Wu
Zhenzhong
Z
Wang
Lianming
L
Xu
D000596:Amino Acids / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002020:Buffaloes
D000596:Amino Acids