SMARCA5 methylation and expression in gastric cancer.
Here, we first evaluated SMARCA5 expression and promoter DNA methylation in gastric carcinogenesis. Immunohistochemistry and methylation-specific PCR were analyzed in 19 and 48 normal mucosa and in 52 and 92 gastric cancer samples, respectively. We observed higher immunoreactivity of SMARCA5 in gastric cancer samples than in normal mucosa. Moreover, SMARCA5 promoter methylation was associated with the absence of protein expression. Our findings suggest that SMARCA5 has a potential role in proliferation and malignancy in gastric carcinogenesis.
Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, Brazil.
Carolina Oliveira
CO
Gigek
Luara Carolina Frias
LC
Lisboa
Mariana Ferreira
MF
Leal
Patricia Natalia Oliveira
PN
Silva
Eleonidas Moura
EM
Lima
André Salim
AS
Khayat
Paulo Pimentel
PP
Assumpção
Rommel Rodriguez
RR
Burbano
Marilia de Arruda Cardoso
Mde A
Smith
D000251:Adenosine Triphosphatases / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D000328:Adult; D000368:Aged; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology
D002868:Chromosomal Proteins, Non-Histone; D000251:Adenosine Triphosphatases; C409462:SMARCA5 protein, human