Electrocardiography as a tool for validating myocardial ischemia-reperfusion procedures in mice.
This paper evaluates the modifications induced by ischemia and ischemia-reperfusion in mice after permanent or transient, respectively, ligation of the left coronary artery and establishes a correlation among the extent of ischemia, electrocardiograph features, and infarct size. The left coronary artery was ligated 1 mm distal from the tip of the left auricle. Histologic analysis revealed that 30-min ischemia (n = 9) led to infarction involving 9.7% ± 0.5% of the left ventricle, whereas 1-h ischemia (n = 9) resulted in transmural infarction of 16.1% ± 4.6% of the left ventricle. In contrast, 24-h ischemia (n = 8) and permanent ischemia (n = 8) induced similarly sized infarcts (33% ± 2% and 31.8% ± 0.7%, respectively), suggesting ineffective reperfusion after 24-h ischemia. Electrocardiography revealed that ligation of the left coronary artery led to ST height elevation (204 compared with 14 μV) and QTc prolongation (136 compared with 76 ms). Both parameters rapidly normalized on reperfusion, demonstrating that electrocardiography was important for validating correct ligation and reperfusion. In addition, electrocardiography predicted the severity of the myocardial damage induced by ischemia. Our results show that electrocardiographic changes present after 30-min ischemia were reversed on reperfusion; however, prolonged ischemia induced pathologic electrocardiographic patterns that remained even after reperfusion. The mouse model of myocardial ischemia-reperfusion can be improved by using electrocardiography to validate ligation and reperfusion during surgery and to predict the severity of infarction.
Institute of Cellular Biology and Pathology 'Nicolae Simionescu', Bucharest, Romania.
Mihai B
MB
Preda
Alexandrina
A
Burlacu
D000818:Animals; D003331:Coronary Vessels / Q000601:surgery; D004195:Disease Models, Animal; D004562:Electrocardiography; D008026:Ligation; D051379:Mice; D009203:Myocardial Infarction / Q000209:etiology / Q000473:pathology*; D015428:Myocardial Reperfusion Injury / Q000150:complications*; D013997:Time Factors
Am J Physiol Heart Circ Physiol. 2004 Mar;286(3):H1201-7
14766681
Cardiovasc Res. 1999 Mar;41(3):586-93
10435030
Am J Physiol Heart Circ Physiol. 2000 Apr;278(4):H1049-55
10749697
Am J Physiol Heart Circ Physiol. 2006 Nov;291(5):H2533-40
16766632
Physiol Rev. 2005 Oct;85(4):1373-416
16183916
Am J Physiol. 1995 Dec;269(6 Pt 2):H2147-54
8594926
J Pharmacol Toxicol Methods. 2002 Nov-Dec;48(3):161-9
14986865
Cardiovasc Res. 2007 Apr 1;74(1):29-38
17188668
Am J Physiol. 1999 Aug;277(2 Pt 2):H660-8
10444492
J Appl Physiol (1985). 2007 Jun;102(6):2104-11
17347379
Cardiovasc Pathol. 2004 Mar-Apr;13(2):91-7
15033158
Physiol Genomics. 2004 Feb 13;16(3):349-60
14679301
Cardiovasc Pathol. 2006 Mar-Apr;15(2):83-90
16533696
Cardiovasc Res. 2000 Jan 1;45(1):231-7
10728340
Circ Res. 2000 Nov 10;87(10):837-9
11073877
Am J Physiol. 1997 Nov;273(5):H2508-19
9374791