Evidence for OTUD-6B participation in B lymphocytes cell cycle after cytokine stimulation.
Deubiquitinating enzymes (DUBs) are important regulators of cell proliferation. Here we identified a functional deubiquitinating enzyme, ovarian tumor domain-containing 6B (OTUD-6B). Mutation of the conserved Cys residue abolished its deubiquitinating activity in vitro. Otud-6b expression was induced with cytokine stimulation in both mouse Ba/F3 cells and primary B lymphocytes followed a rapid decrease. This rapid decrease was partially facilitated by tristetraprolin (TTP) destabilization of Otud-6b mRNA through AU-rich motifs. Enforced expression of OTUD-6B in Ba/F3 cells could block cell proliferation by arresting cells in G1 phase. In addition, cyclin D2 level was down-regulated when OTUD-6B WT was overexpressed. Therefore, down-regulation of Otud-6b expression after prolonged cytokine stimulation may be required for cell proliferation in B lymphocytes.
The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Zhongping
Z
Xu
Yufang
Y
Zheng
Yufei
Y
Zhu
Xiangyin
X
Kong
Landian
L
Hu
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D015536:Down-Regulation; D010450:Endopeptidases / Q000235:genetics / Q000502:physiology*; D006801:Humans; D051379:Mice; D012333:RNA, Messenger
D016207:Cytokines; D012333:RNA, Messenger; D010450:Endopeptidases; C559912:OTUD6B protein, human
Mol Microbiol. 1993 Jul;9(1):1-7
7692215
Cell. 2001 Nov 16;107(4):451-64
11719186
J Cell Biochem. 2007 Apr 15;100(6):1477-92
17133347
Biochim Biophys Acta. 2004 Nov 29;1695(1-3):189-207
15571815
RNA. 2008 May;14(5):888-902
18367721
Science. 2003 Mar 7;299(5612):1540
12624257
Science. 1996 Dec 6;274(5293):1672-7
8939849
Methods Mol Biol. 2004;249:177-92
14573900
J Exp Med. 2006 Oct 30;203(11):2551-62
17060474
Genes Dev. 2001 Aug 15;15(16):2042-7
11511535
PLoS One. 2007 Jul 25;2(7):e679
17653289
J Biol Chem. 1997 Jan 3;272(1):51-7
8995226
Curr Opin Cell Biol. 2008 Apr;20(2):156-63
18346885
Mol Cell Biol. 1996 Sep;16(9):4808-17
8756639
J Biol Chem. 2004 Apr 2;279(14):13993-4000
14699124
FEBS Lett. 2001 Feb 16;490(3):179-89
11223033
J Biol Chem. 2004 Jan 23;279(4):2368-76
14583620
Cell. 2009 Jul 23;138(2):389-403
19615732
Nature. 1995 Oct 19;377(6550):591-4
7566171
Science. 1998 Aug 14;281(5379):1001-5
9703499
Biochem Biophys Res Commun. 2001 Apr 13;282(4):853-60
11352628
Nat Rev Mol Cell Biol. 2009 Aug;10(8):550-63
19626045
EMBO J. 2002 Sep 2;21(17):4709-18
12198173
Genome Res. 2006 Jan;16(1):55-65
16344560
Immunol Today. 1996 May;17(5):206-8
8991379
Science. 2007 Dec 7;318(5856):1628-32
17991829
J Invest Dermatol. 2008 Mar;128(3):587-93
17851586
J Allergy Clin Immunol. 2007 Dec;120(6):1292-300
18073124
J Biol Chem. 1996 May 24;271(21):12179-84
8647811
Adv Immunol. 2006;92:225-305
17145306
Exp Hematol. 2007 Sep;35(9):1456-65
17761290
J Biol Chem. 1998 Mar 13;273(11):6417-23
9497373
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3275-9
8622927
Genomics. 2005 Apr;85(4):524-9
15780755
Nat Rev Mol Cell Biol. 2003 Mar;4(3):192-201
12612638
Cytokine Growth Factor Rev. 2007 Feb-Apr;18(1-2):73-83
17336579
Mol Biol Cell. 2000 Nov;11(11):3835-48
11071910
Leukemia. 2006 Dec;20(12):2208-10
17051239
Semin Immunol. 2006 Feb;18(1):20-30
16303314
Nat Protoc. 2006;1(2):577-80
17406284
Nat Rev Cancer. 2006 Feb;6(2):99-106
16491069
Cell. 2006 Oct 6;127(1):99-111
17018280
Dev Cell. 2007 Sep;13(3):446-54
17765686
Oncogene. 2007 Apr 12;26(17):2471-82
17043650
Cell. 1986 Aug 29;46(5):659-67
3488815