Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters.
We demonstrate an optical Fourier filtering method which can be used to characterize subcellular morphology during dynamic cellular function. In this paper, our Fourier filters were based on two-dimensional Gabor elementary functions, which can be tuned to sense directly object size and orientation. We utilize this method to quantify changes in mitochondrial and nuclear structure during the first three hours of apoptosis. We find that the technique is sensitive to a decrease in particle orientation consistent with apoptosis-induced mitochondrial fragmentation. The scattering signal changes were less pronounced in the nucleus and the remainder of the cytoplasm. Particles in these regions were less oriented than mitochondria and did not change orientation significantly.
Department of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.
Robert M
RM
Pasternack
Bryan
B
Rabin
Jing-Yi
JY
Zheng
Nada N
NN
Boustany
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