Hyaluronic acid biodegradable material for reconstruction of vascular wall: a preliminary study in rats.
The objective of this preliminary study was to develop a reabsorbable vascular patch that did not require in vitro cell or biochemical preconditioning for vascular wall repair. Patches were composed only of hyaluronic acid (HA). Twenty male Wistar rats weighing 250-350 g were used. The abdominal aorta was exposed and isolated. A rectangular breach (1 mm × 5 mm) was made on vessel wall and arterial defect was repaired with HA made patch. Performance was assessed at 1, 2, 4, 8, and 16 weeks after surgery by histology and immunohistochemistry. Extracellular matrix components were evaluated by molecular biological methods. After 16 weeks, the biomaterial was almost completely degraded and replaced by a neoartery wall composed of endothelial cells, smooth muscle cells, collagen, and elastin fibers organized in layers. In conclusion, HA patches provide a provisional three-dimensional support to interact with cells for the control of their function, guiding the spatially and temporally multicellular processes of artery regeneration.
Clinic of Plastic and Reconstructive Surgery, University of Padova, Padova, Italy.
Laura
L
Pandis
Barbara
B
Zavan
Franco
F
Bassetto
Letizia
L
Ferroni
Laura
L
Iacobellis
Giovanni
G
Abatangelo
Sandro
S
Lepidi
Roberta
R
Cortivo
Vincenzo
V
Vindigni
D020341:Absorbable Implants; D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology / Q000293:injuries / Q000502:physiology / Q000601:surgery; D015415:Biomarkers / Q000032:analysis; D015870:Gene Expression; D048091:Guided Tissue Regeneration / Q000295:instrumentation* / Q000379:methods; D006820:Hyaluronic Acid; D008297:Male; D010865:Pilot Projects; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014945:Wound Healing
D015415:Biomarkers; D006820:Hyaluronic Acid