Structural and mechanical properties of Klebsiella pneumoniae type 3 Fimbriae.
This study investigated the structural and mechanical properties of Klebsiella pneumoniae type 3 fimbriae, which constitute a known virulence factor for the bacterium. Transmission electron microscopy and optical tweezers were used to understand the ability of the bacterium to survive flushes. An individual K. pneumoniae type 3 fimbria exhibited a helix-like structure with a pitch of 4.1 nm and a three-phase force-extension curve. The fimbria was first nonlinearly stretched with increasing force. Then, it started to uncoil and extended several micrometers at a fixed force of 66 ± 4 pN (n = 22). Finally, the extension of the fimbria shifted to the third phase, with a characteristic force of 102 ± 9 pN (n = 14) at the inflection point. Compared with the P fimbriae and type 1 fimbriae of uropathogenic Escherichia coli, K. pneumoniae type 3 fimbriae have a larger pitch in the helix-like structure and stronger uncoiling and characteristic forces.
Institute and Department of Electrophysics, National Chiao Tung University, 1001 University Road, Hsinchu 300, Taiwan, Republic of China.
Feng-Jung
FJ
Chen
Chia-Han
CH
Chan
Ying-Jung
YJ
Huang
Kuo-Liang
KL
Liu
Hwei-Ling
HL
Peng
Hwan-You
HY
Chang
Gunn-Guang
GG
Liou
Tri-Rung
TR
Yew
Cheng-Hsien
CH
Liu
Ken Y
KY
Hsu
Long
L
Hsu
D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism*; D019563:Mechanics; D008855:Microscopy, Electron, Scanning; D011487:Protein Conformation; D056689:Uropathogenic Escherichia coli / Q000378:metabolism; D037521:Virulence Factors
D037521:Virulence Factors; D036781:Fimbriae Proteins
Infect Immun. 1992 Apr;60(4):1577-88
1312518
Nature. 1995 Jan 12;373(6510):164-7
7816100
EMBO Rep. 2005 Jan;6(1):52-6
15592451
Curr Opin Struct Biol. 2000 Oct;10(5):548-56
11042452
Biophys J. 2006 Mar 1;90(5):1521-34
16361334
PLoS Biol. 2006 Sep;4(9):e298
16933977
J Bacteriol. 1984 Jan;157(1):330-3
6140260
Science. 1999 Aug 13;285(5430):1061-6
10446051
Biophys J. 1998 Jan;74(1):623-32
9449363
Biochem Biophys Res Commun. 2006 Nov 10;350(1):33-8
16997275
Infect Immun. 2009 Nov;77(11):5016-24
19703972
J Bacteriol. 2008 Feb;190(3):1054-63
18055599
Biophys J. 2001 Oct;81(4):1897-906
11566764
Kidney Int. 2007 Jul;72(1):19-25
17396114
Mol Microbiol. 1990 Aug;4(8):1353-61
1980713
Biophys J. 1997 Mar;72(3):1335-46
9138579
Biophys J. 2006 Nov 15;91(10):3848-56
16950852
Cell Host Microbe. 2009 Jun 18;5(6):580-92
19527885
J Mol Biol. 1996 Dec 20;264(5):919-32
9000621
Biochem Biophys Res Commun. 2006 Nov 24;350(3):537-42
17022945
Rev Sci Instrum. 2010 Jul;81(7):075103
20687755
Structure. 1994 Jul 15;2(7):641-9
7922041
J Bacteriol. 1988 Aug;170(8):3547-53
2900237
Science. 1994 Sep 9;265(5178):1599-600
8079175
Eur J Biochem. 1984 Sep 3;143(2):395-9
6147250
Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9861-6
16782819
Biophys J. 2004 Dec;87(6):4271-83
15377509
Biophys J. 2007 Nov 1;93(9):3008-14
17675342
Cell. 2005 May 20;121(4):515-527
15907466
J Bacteriol. 1999 Feb;181(4):1059-71
9973330
Emerg Infect Dis. 2008 Oct;14(10):1592-600
18826824
Biophys J. 2007 Mar 15;92(6):2230-6
17172309
Mol Microbiol. 2004 Sep;53(5):1545-57
15387828
Infect Immun. 1997 Apr;65(4):1546-9
9119502
Infect Immun. 2005 Nov;73(11):7588-96
16239562
Eur Biophys J. 2010 Jul;39(8):1105-15
19885656
J Infect Dis. 2001 Mar 1;183 Suppl 1:S36-40
11171011
Nature. 1982 Jul 8;298(5870):131-5
7201078
Chemphyschem. 2009 Jul 13;10(9-10):1533-40
19565578
Biochim Biophys Acta. 2004 Nov 11;1694(1-3):259-67
15546670
Cell Microbiol. 2001 Apr;3(4):255-64
11298649
J Mol Biol. 2002 Nov 8;323(5):845-57
12417198
J Gen Microbiol. 1959 Aug;21:271-86
13818511