Toxic responses in primary rat hepatocytes exposed with occupational dust collected from work environment of bone-based industrial unit.
In this in vitro study we investigated the toxic responses in hepatocytes treated with occupational dust to which workers are exposed in bone-based industrial units. The present study investigated the toxicity mechanism of bone-based occupational dust, from a particular industrial unit, on isolated rat hepatocytes. The hepatocytes were isolated by collagenase perfusion method and cell viability was determined by trypan blue exclusion and MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay treated with occupational dust at 0.1-1.0 mgmL(-1), for 120 min. The cell viability decreased significantly in a concentration-dependent manner. Dust induced significant membrane damage measured by lactate dehydrogenase (LDH) and glutathione (GSH) release in culture media for 30-, 60- and 120 min treatment duration. The toxicity was found to be correlated with the induction of lipid peroxidation (LPO). In addition, nitric oxide (NO), and hydrogen peroxide (H(2)O(2)) generation by occupational dusts were also found to be time- and concentration-dependent. Over all the present study provides initial evidences for the toxic potential of occupational dust generated in bone-based industries and, therefore, the dust exposure to workers in unorganized industrial units should be controlled.
Fibre Toxicology Division, Indian Institute of Toxicology Research (CSIR), Lucknow 226 001, UP, India. iqbal@iitr.res.in
Iqbal
I
Ahmad
Huma
H
Siddiqui
Mohd Javed
MJ
Akhtar
Mohd Imran
MI
Khan
Govil
G
Patil
Mohd
M
Ashquin
Devendra Kumar
DK
Patel
Jamal Mohd
JM
Arif
D000395:Air Pollutants, Occupational / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D001842:Bone and Bones; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004391:Dust / Q000032:analysis*; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007221:Industry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity; D009569:Nitric Oxide / Q000378:metabolism; D016273:Occupational Exposure / Q000032:analysis / Q000706:statistics & numerical data; D051381:Rats; D017208:Rats, Wistar; D012613:Sculpture; D017132:Workplace
D000395:Air Pollutants, Occupational; D004391:Dust; D019216:Metals, Heavy; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D005978:Glutathione