DNA-based methods in the immunohematology reference laboratory.
Although hemagglutination serves the immunohematology reference laboratory well, when used alone, it has limited capability to resolve complex problems. This overview discusses how molecular approaches can be used in the immunohematology reference laboratory. In order to apply molecular approaches to immunohematology, knowledge of genes, DNA-based methods, and the molecular bases of blood groups are required. When applied correctly, DNA-based methods can predict blood groups to resolve ABO/Rh discrepancies, identify variant alleles, and screen donors for antigen-negative units. DNA-based testing in immunohematology is a valuable tool used to resolve blood group incompatibilities and to support patients in their transfusion needs.
Laboratory of Immunochemistry, New York Blood Center, 310 East 67th Street, New York, NY 10065, United States. mreid@nybloodcenter.org
Marion E
ME
Reid
Gregory A
GA
Denomme
D000328:Adult; D000486:Allergy and Immunology; D001771:Blood Banks; D001789:Blood Group Antigens / Q000032:analysis / Q000235:genetics*; D001787:Blood Group Incompatibility / Q000175:diagnosis / Q000235:genetics; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001803:Blood Transfusion; D004247:DNA / Q000235:genetics*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006402:Hematologic Diseases / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D006405:Hematology / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D007258:Informed Consent; D007753:Laboratories; D025202:Molecular Diagnostic Techniques; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood; D012015:Reference Standards
D001789:Blood Group Antigens; D004247:DNA
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