Entianin, a novel subtilin-like lantibiotic from Bacillus subtilis subsp. spizizenii DSM 15029T with high antimicrobial activity.
Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously reported subtilin autoinduction bioassay in combination with mass spectrometric analyses to identify the novel subtilin-like lantibiotic entianin from Bacillus subtilis subsp. spizizenii DSM 15029(T). Linearization of entianin using Raney nickel-catalyzed reductive cleavage enabled, for the first time, the use of tandem mass spectrometry for the fast and efficient determination of an entire lantibiotic primary structure, including posttranslational modifications. The amino acid sequence determined was verified by DNA sequencing of the etnS structural gene, which confirmed that entianin differs from subtilin at 3 amino acid positions. In contrast to B. subtilis ATCC 6633, which produces only small amounts of unsuccinylated subtilin, B. subtilis DSM 15029(T) secretes considerable amounts of unsuccinylated entianin. Entianin was very active against several Gram-positive pathogens, such as Staphylococcus aureus and Enterococcus faecalis. The growth-inhibiting activity of succinylated entianin (S-entianin) was much lower than that of unsuccinylated entianin: a 40-fold higher concentration was required for inhibition. For succinylated subtilin (S-subtilin), a concentration 100-fold higher than that of unsuccinylated entianin was required to inhibit the growth of a B. subtilis test strain. This finding was in accordance with a strongly reduced sensing of cellular envelope stress provided by S-entianin relative to that of entianin. Remarkably, S-entianin and S-subtilin showed considerable autoinduction activity, clearly demonstrating that autoinduction and antibiotic activity underlie different molecular mechanisms.
Institute for Molecular Biosciences, Max-von-Laue-Str. 9, Goethe University Frankfurt, D-60438 Frankfurt, Germany.
Sebastian W
SW
Fuchs
Thorsten W
TW
Jaskolla
Sophie
S
Bochmann
Peter
P
Kötter
Thomas
T
Wichelhaus
Michael
M
Karas
Torsten
T
Stein
Karl-Dieter
KD
Entian
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry*; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D053898:Biosynthetic Pathways / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects*; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000187:drug effects*; D053719:Tandem Mass Spectrometry
D001430:Bacteriocins; D004269:DNA, Bacterial
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