Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation.
We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles.
Maciej
M
Antkowiak
Maria Leilani
ML
Torres-Mapa
Kishan
K
Dholakia
Frank J
FJ
Gunn-Moore
Methods Cell Biol. 2007;82:113-51
17586256
J R Soc Interface. 2010 Jun 6;7(47):863-71
20064901
J Biomed Opt. 2008 Sep-Oct;13(5):050508
19021378
J Biomed Opt. 2007 Sep-Oct;12(5):054010
17994898
Trends Biochem Sci. 2002 Jan;27(1):27-33
11796221
Opt Lett. 2009 Mar 15;34(6):767-9
19282926
Opt Express. 2005 Nov 14;13(23):9361-73
19503137
Biotechnol Bioeng. 2007 Dec 15;98(6):1230-41
17615558
J Biomed Opt. 2007 Nov-Dec;12(6):064002
18163818
Opt Express. 2008 Jun 23;16(13):9357-64
18575499
Appl Opt. 2008 Jul 1;47(19):D176-82
18594573
Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6731-6
20351261
Opt Express. 2009 Jul 6;17(14):12031-8
19582118
Opt Express. 2006 Jun 26;14(13):5895-908
19516759
J Biomed Opt. 2010 Jul-Aug;15(4):041506
20799784
Opt Lett. 1999 Mar 1;24(5):291-3
18071483
Blood Cells Mol Dis. 2008 Jul-Aug;41(1):10-6
18387320
Biomed Eng Online. 2006 Mar 23;5:21
16556319
J Biomed Opt. 2009 Sep-Oct;14(5):054040
19895141
J Biophotonics. 2010 Oct;3(10-11):696-705
20583035
Opt Express. 2008 Mar 3;16(5):3021-31
18542388
Appl Opt. 2003 Apr 10;42(11):1938-46
12699340
Phys Rev Lett. 2008 Jan 25;100(3):038102
18233040
Blood Cells Mol Dis. 2009 May-Jun;42(3):228-32
19324576
Nat Methods. 2006 Jun;3(6):455-60
16721379
Nature. 2002 Jul 18;418(6895):290-1
12124612
Proc Natl Acad Sci U S A. 2009 May 5;106(18):7624-9
19380745
J Biophotonics. 2008 Aug;1(3):183-99
19412968
J Biophotonics. 2009 Dec;2(12):736-43
19603388
Am J Physiol Cell Physiol. 2008 Aug;295(2):C538-44
18562484
J Biomed Opt. 2010 Jan-Feb;15(1):010505
20210420
J Biomed Opt. 2010 Jul-Aug;15(4):041507
20799785
Science. 2003 Apr 4;300(5616):82-6
12677057
Opt Lett. 2008 Jul 15;33(14):1626-8
18628819