A rapid and sensitive method to identify and differentiate Salmonella enterica serotype Typhimurium and Salmonella enterica serotype 4,[5],12:i:- by combining traditional serotyping and multiplex polymerase chain reaction.
Salmonella enterica subspecies enterica serotype 4,[5],12:i:- is an emerging serovar considered as a monophasic variant of Salmonella enterica serotype Typhimurium. The antigenic and genetic similarity between Salmonella 4,[5],12:i:- and Salmonella Typhimurium suggests that they may behave in a similar way and represent a comparable threat to public health. As serotyping alone does not necessarily provide for identification of Salmonella 4,[5],12:i:- and its differentiation from Salmonella Typhimurium, a method that combines traditional serotyping and a multiplex polymerase chain reaction has been tested on 208 strains serotyped as Salmonella 4,[5],12:i:-, Salmonella Typhimurium, and similar serovars of serogroup B sharing the same phase-1 antigen "i." For 191 strains, the combined method fully confirmed the results provided by traditional serotyping, whereas for 17 strains of Salmonella 4,[5],12:i:- and Salmonella Typhimurium some inconsistencies emerged between the two methods. The combined method resulted in a more accurate and faster identification of these two relevant serovars.
OIE, National Reference Laboratory for Salmonella, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, Padova, Italy.
Lisa
L
Barco
Antonia Anna
AA
Lettini
Elena
E
Ramon
Alessandra
A
Longo
Cristina
C
Saccardin
Maria Cristina Dalla
MC
Pozza
Antonia
A
Ricci
D000942:Antigens, Bacterial / Q000032:analysis; D015373:Bacterial Typing Techniques; D021821:Communicable Diseases, Emerging / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D003937:Diagnosis, Differential; D005060:Europe / Q000453:epidemiology; D006801:Humans; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012480:Salmonella Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D017384:Sequence Deletion; D012703:Serotyping
D000942:Antigens, Bacterial; D004269:DNA, Bacterial; D021901:DNA, Intergenic
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