Comparative genome-wide analysis of small RNAs of major Gram-positive pathogens: from identification to application.
In the recent years, the number of drug- and multi-drug-resistant microbial strains has increased rapidly. Therefore, the need to identify innovative approaches for development of novel anti-infectives and new therapeutic targets is of high priority in global health care. The detection of small RNAs (sRNAs) in bacteria has attracted considerable attention as an emerging class of new gene expression regulators. Several experimental technologies to predict sRNA have been established for the Gram-negative model organism Escherichia coli. In many respects, sRNA screens in this model system have set a blueprint for the global and functional identification of sRNAs for Gram-positive microbes, but the functional role of sRNAs in colonization and pathogenicity for Listeria monocytogenes, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis and Clostridium difficile is almost completely unknown. Here, we report the current knowledge about the sRNAs of these socioeconomically relevant Gram-positive pathogens, overview the state-of-the-art high-throughput sRNA screening methods and summarize bioinformatics approaches for genome-wide sRNA identification and target prediction. Finally, we discuss the use of modified peptide nucleic acids (PNAs) as a novel tool to inactivate potential sRNA and their applications in rapid and specific detection of pathogenic bacteria.
Institute of Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, 35392 Giessen, Germany.
Mobarak A
MA
Mraheil
André
A
Billion
Carsten
C
Kuenne
Jordan
J
Pischimarov
Bernd
B
Kreikemeyer
Susanne
S
Engelmann
Axel
A
Hartke
Jean-Christophe
JC
Giard
Maja
M
Rupnik
Sonja
S
Vorwerk
Markus
M
Beier
Julia
J
Retey
Thomas
T
Hartsch
Anette
A
Jacob
Franz
F
Cemič
Jürgen
J
Hemberger
Trinad
T
Chakraborty
Torsten
T
Hain
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods; D001688:Biological Products / Q000235:genetics / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D016372:RNA, Antisense / Q000235:genetics / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001688:Biological Products; D016372:RNA, Antisense; D034741:RNA, Small Interfering
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