Modulation of inflammatory markers by the ethanolic extract of Leucas aspera in adjuvant arthritis.
OBJECTIVE
To investigate the anti-inflammatory and antioxidant potential of ethanolic extract of Leucas aspera (EELA) in adjuvant arthritis.


METHODS
Complete Freund's adjuvant served to induce arthritis. EELA was administered in two doses along with vehicle control (0.1% carboxymethyl cellulose) and positive control (Diclofenac). Levels of tumour necrosis factor (TNF-)-α, C-reactive protein (CRP), Interleukin-2 (IL-2), Cathepsin D, activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were estimated in plasma/hemolysate and tissue. HPLC analysis of EELA was also performed.


RESULTS
EELA exhibited significant anti-inflammatory (p<0.001) and antioxidant activity (p<0.001). It did not show mortality up to 2000 mg/kg body weight. Histopathological studies confirmed complete cartilage regeneration and near normal joint in EELA2 treated arthritic rats.


CONCLUSIONS
This study highlighted the antioxidant and anti-inflammatory potential of Leucas aspera. Three major families of compounds present in EELA may explain these activities: catechins (epicatechin, beta epicatechin), flavonoids (procyanidin), phytosterols (beta-sitosterol) apart from glycosides, phenolic compounds and tannins.
Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai 600117, India.
K G
KG
Kripa
D
D
Chamundeeswari
J
J
Thanka
C
C
Uma Maheswara Reddy
D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D015415:Biomarkers / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism*; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D018836:Inflammation Mediators; D010936:Plant Extracts; D000431:Ethanol