Functional interaction between Foxd3 and Pax3 in cardiac neural crest development.
The transcription factors Foxd3 and Pax3 are important early regulators of neural crest (NC) progenitor cell properties. Homozygous mutations of Pax3 or a homozygous NC-specific deletion of Foxd3 cause marked defects in most NC derivatives, but neither loss of both Foxd3 alleles nor loss of one Pax3 allele alone greatly affects overall development of cardiac NC derivatives. In contrast, compound mutant embryos homozygous for a NC-specific Foxd3 mutation and heterozygous for Pax3 have fully penetrant persistent truncus arteriosus, severe thymus hypoplasia, and midgestation lethality. Foxd3; Pax3 compound mutant embryos have increased cell death in the neural folds and a drastic early reduction of NC cells, with an almost complete absence of NC caudal to the first pharyngeal arch. The genetic interaction between these genes implicates gene dosage-sensitive roles for Foxd3 and Pax3 in cardiac NC progenitors. Foxd3 and Pax3 act together to affect survival and maintenance of cardiac NC progenitors, and loss of these progenitors catastrophically affects key aspects of later cardiovascular development.
Vanderbilt University Medical Center, Department of Cell and Developmental Biology, Center for Stem Cell Biology, Program in Developmental Biology, Nashville, Tennessee, USA.
Brian L
BL
Nelms
Elise R
ER
Pfaltzgraff
Patricia A
PA
Labosky
D000818:Animals; D019465:Craniofacial Abnormalities / Q000235:genetics; D020964:Embryo Loss / Q000235:genetics; D051858:Forkhead Transcription Factors / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D009432:Neural Crest / Q000166:cytology / Q000254:growth & development*; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D017384:Sequence Deletion; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology; D013950:Thymus Gland / Q000002:abnormalities; D014339:Truncus Arteriosus, Persistent / Q000235:genetics*
D051858:Forkhead Transcription Factors; C101757:Foxd3 protein, mouse; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; C068686:Pax3 protein, mouse
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