Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7.
The colonization process of Olea europaea by the defoliating pathotype of Verticillium dahliae, and the in planta interaction with the endophytic, biocontrol strain Pseudomonas fluorescens PICF7 were determined. Differential fluorescent protein tagging was used for the simultaneous visualization of P. fluorescens PICF7 and V. dahliae in olive tissues. Olive plants were bacterized with PICF7 and then transferred to V. dahliae-infested soil. Monitoring olive colonization events by V. dahliae and its interaction with PICF7 was conducted using a non-gnotobiotic system, confocal laser scanner microscopy and tissue vibratoming sections. A yellow fluorescently tagged V. dahliae derivative (VDAT-36I) was obtained by Agrobacterium tumefaciens-mediated transformation. Isolate VDAT-36I quickly colonized olive root surface, successfully invaded root cortex and vascular tissues via macro- and micro-breakages, and progressed to the aerial parts of the plant through xylem vessel cells. Strain PICF7 used root hairs as preferred penetration site, and once established on/in root tissues, hindered pathogen colonization. For the first time using this approach, the entire colonization process of a woody plant by V. dahliae is reported. Early and localized root surface and root endophytic colonization by P. fluorescens PICF7 is needed to impair full progress of verticillium wilt epidemics in olive.
Departamento de Mejora Genética, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Alameda del Obispo s/n, Apartado 4084, 14080 Córdoba, Spain.
Pilar
P
Prieto
Carmen
C
Navarro-Raya
Antonio
A
Valverde-Corredor
Stefan G
SG
Amyotte
Katherine F
KF
Dobinson
Jesús
J
Mercado-Blanco
D000898:Antibiosis; D018613:Microscopy, Confocal; D031658:Olea / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000502:physiology*; D020172:Verticillium / Q000254:growth & development* / Q000472:pathogenicity*
Mol Plant Microbe Interact. 2000 Nov;13(11):1170-6
11059483
Fungal Genet Biol. 2003 Feb;38(1):54-62
12553936
Phytopathology. 2001 Feb;91(2):173-80
18944391
Mol Plant Microbe Interact. 2006 Aug;19(8):827-37
16903349
Phytopathology. 2008 Aug;98(8):871-85
18943205
Mol Plant Microbe Interact. 2001 Feb;14(2):255-60
11204790
Appl Environ Microbiol. 2001 Nov;67(11):5285-93
11679357
Mol Plant Microbe Interact. 2003 Nov;16(11):983-93
14601666
Fungal Genet Biol. 2008 Dec;45(12):1525-32
18951989
FEMS Microbiol Ecol. 2006 Aug;57(2):302-10
16867147
J Agric Food Chem. 2007 May 2;55(9):3373-7
17394331
Appl Environ Microbiol. 2005 Apr;71(4):1685-93
15811990
Curr Genet. 2004 Feb;45(2):104-10
14618375
Antonie Van Leeuwenhoek. 2007 Nov;92(4):367-89
17588129
Phytopathology. 2006 May;96(5):485-95
18944308
Nat Protoc. 2007;2(7):1831-8
17641652
Plant Dis. 2003 Dec;87(12):1487-1494
30812391
FEMS Microbiol Ecol. 2008 May;64(2):297-306
18336554