Thy-1 (CD90) regulates the extravasation of leukocytes during inflammation.
Human Thy-1 (CD90) has been shown to mediate adhesion of inflammatory cells to activated microvascular endothelial cells via interaction with Mac-1 (CD11b/CD18) in vitro. Since there are no data showing the physiological relevance of Thy-1 for the recruitment of inflammatory cells in vivo, different inflammation models were investigated in Thy-1-deficient mice and littermate controls. In thioglycollate-induced peritonitis, the number of neutrophils and monocytes was significantly diminished in Thy-1-deficient mice. During acute lung inflammation, the extravasation of eosinophils and monocytes into the lung was significantly reduced in Thy-1-deficient mice. Moreover, during chronic lung inflammation, the influx of eosinophils and monocytes was also strongly decreased. These effects were independent of Thy-1 expression on T cells, as shown by the transplantation of WT BM into the Thy-1-deficient mice. In spite of the strong Thy-1 expression on T cells in the chimeric mice, the extravasation of the inflammatory cells in these mice was significantly diminished, compared to control mice. Finally, the altered number and composition of infiltrating leukocytes in Thy-1-deficient mice modified the chemokine/cytokine and protease expression at the site of inflammation. In conclusion, Thy-1 is involved in the control of inflammatory cell recruitment and, thus, also in conditioning the inflammatory microenvironment.
Department of Dermatology, Venerology and Allergology, Medical Faculty of Leipzig University, Leipzig, Germany.
Kathleen
K
Schubert
Tobias
T
Polte
Ulrike
U
Bönisch
Sina
S
Schader
Rafaela
R
Holtappels
Guido
G
Hildebrandt
Jörg
J
Lehmann
Jan C
JC
Simon
Ulf
U
Anderegg
Anja
A
Saalbach
D000818:Animals; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D018925:Chemokines / Q000378:metabolism; D042783:Endothelial Cells / Q000276:immunology / Q000473:pathology; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D007378:Interleukins / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology* / Q000473:pathology / Q000502:physiology; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000276:immunology / Q000473:pathology; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D010447:Peptide Hydrolases / Q000378:metabolism; D010538:Peritonitis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D011014:Pneumonia / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D018800:Thy-1 Antigens / Q000235:genetics / Q000378:metabolism*; D018183:Transplantation Chimera / Q000276:immunology
D018925:Chemokines; D007378:Interleukins; D018800:Thy-1 Antigens; D010447:Peptide Hydrolases