Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity.
BACKGROUND
carbon nanotubes (CNT) can have adverse effects on health. Therefore, minimizing the risk associated with CNT exposure is of crucial importance. The aim of this work was to evaluate if coating multi-walled CNT (MWCNT) with polymers could modify their toxicity, thus representing a useful strategy to decrease adverse health effects of CNT. We used industrially-produced MWCNT uncoated (NT1) or coated (50/50 wt%) with acid-based (NT2) or polystyrene-based (NT3) polymer, and exposed murine macrophages (RAW 264.7 cell line) or Balb/c mice by intratracheal administration. Biological experiments were performed both in vitro and in vivo, examining time- and dose-dependent effects of CNT, in terms of cytotoxicity, expression of genes and proteins related to oxidative stress, inflammation and tissue remodeling, cell and lung tissue morphology (optical and transmission electron microscopy), and bronchoalveolar lavage fluid content analysis.


RESULTS
extensive physico-chemical characterization of MWCNT was performed, and showed, although similar dimensions for the 3 MWCNT, a much smaller specific surface area for NT2 and NT3 as compared to NT1 (54.1, 34 and 227.54 m(2)/g respectively), along with different surface characteristics. MWCNT-induced cytotoxicity, oxidative stress, and inflammation were increased by acid-based and decreased by polystyrene-based polymer coating both in vitro in murine macrophages and in vivo in lung of mice monitored for 6 months.


CONCLUSIONS
these results demonstrate that coating CNT with polymers, without affecting their intrinsic structure, may constitute a useful strategy for decreasing CNT toxicity, and may hold promise for improving occupational safety and that of general the user.
INSERM U, Créteil, France.
Lyes
L
Tabet
Cyrill
C
Bussy
Ari
A
Setyan
Angélique
A
Simon-Deckers
Michel J
MJ
Rossi
Jorge
J
Boczkowski
Sophie
S
Lanone
D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D001672:Biocompatible Materials; D002470:Cell Survival / Q000187:drug effects; D019570:Inhalation Exposure; D007442:Intubation, Intratracheal; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D009784:Occupational Diseases; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D011137:Polystyrenes / Q000737:chemistry*
D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D011137:Polystyrenes
Exp Lung Res. 1999 Jan-Feb;25(1):1-6
10027075
Environ Health Perspect. 2007 Aug;115(8):1125-31
17687437
Environ Health Perspect. 2007 Mar;115(3):377-82
17431486
Chem Res Toxicol. 2008 Sep;21(9):1690-7
18636755
Science. 2006 Feb 3;311(5761):622-7
16456071
Nanomedicine. 2009 Dec;5(4):424-31
19341817
Nano Lett. 2006 Jun;6(6):1261-8
16771591
Toxicol Appl Pharmacol. 2005 Sep 15;207(3):221-31
16129115
Nanotoxicology. 2010 Jun;4(2):207-46
20795897
Chem Res Toxicol. 2007 Oct;20(10):1426-33
17883261
Toxicol In Vitro. 2007 Apr;21(3):438-48
17125965
Am J Respir Crit Care Med. 2001 Nov 1;164(9):1665-8
11719307
Part Fibre Toxicol. 2009 Dec 31;6:35
20043844
Nat Nanotechnol. 2008 Jul;3(7):423-8
18654567
Nanomedicine (Lond). 2009 Jun;4(4):373-5
19505238
Am J Respir Crit Care Med. 2002 Nov 1;166(9):1240-7
12403694
Toxicol Sci. 2008 Jan;101(1):4-21
17602205
Am J Physiol Lung Cell Mol Physiol. 2007 Nov;293(5):L1230-9
17766584
Toxicology. 2010 Jun 4;272(1-3):39-45
20381574
Toxicol Lett. 2007 Jan 30;168(2):121-31
17169512
Am J Respir Crit Care Med. 2009 Jul 1;180(1):29-35
19324973
Nanomedicine. 2011 Feb;7(1):40-9
20620235
Toxicol Appl Pharmacol. 2009 May 1;236(3):276-81
19233222
Biomacromolecules. 2006 Mar;7(3):755-60
16529411
Nat Nanotechnol. 2009 Nov;4(11):708-10
19893519
Am J Physiol Lung Cell Mol Physiol. 2008 Oct;295(4):L552-65
18658273
Biomaterials. 1997 Jan;18(1):69-74
9003900
J Toxicol Environ Health A. 2009;72(2):60-73
19034795
Annu Rev Pharmacol Toxicol. 2010;50:63-88
20055698
Pharmacol Ther. 2008 Oct;120(1):35-42
18703086
Small. 2009 Apr;5(4):511-20
19197964
Toxicol Appl Pharmacol. 2009 Sep 15;239(3):224-32
19481103
Phys Chem Chem Phys. 2009 Aug 7;11(29):6205-17
19606331
Am J Respir Crit Care Med. 2002 Oct 1;166(7):998-1004
12359661
Biomaterials. 2007 Jul;28(20):3074-82
17428532
Nano Lett. 2008 Sep;8(9):2659-63
18672943
Toxicology. 2009 Jun 16;260(1-3):142-9
19464580
Free Radic Biol Med. 2006 Apr 1;40(7):1227-33
16545691
Crit Rev Toxicol. 2006 Mar;36(3):189-217
16686422
J Intern Med. 2010 Jan;267(1):89-105
20059646