[Comparison of reactive oxygen species production in neat semen and washed spermatozoa].
OBJECTIVE
To determine Reactive Oxygen Species (ROS) production in neat semen and spermatozoa suspension using chemiluminescence and to examine correlation between both methods.


METHODS
Prospective laboratory study.


METHODS
Department of Obstetric and Gynecology, University Hospital, Olomouc.


METHODS
The study included fertile volunteers (FV, n = 17), men from infertile couples (NM, n = 19) and men with idiopathic infertility (NMI, n = 15). ROS levels were determined by the same method in neat and washed semen samples.


RESULTS
The ROS production in neat semen was lower than that in spermatozoa suspension. There was no significant diference in ROS production between volunteers and males from infertile couples. There was a significant correlation between log ROS in neat semen and in spermatozoa suspension in studied groups (FV r = 0.85, p = 1.5 x 10(-5); NM r = 0.76, p < 2 x 10(-4); NMI r = 0.75, p < 1.5 x 10(-3)).


CONCLUSIONS
Measurement of ROS in neat semen is simpler, faster and better reflecting the actual level of oxidative stress than the same measurement in spermatozoa suspension. The implementation of this method can complement the algorithm of diagnostics and treatment of male infertility and be helpful in selection of patients for antioxidant or antibiotic treatment.
Gynekologicko-porodnická klinika FN a LF UP Olomouc. svobodom@fnol.cz
M
M
Svobodová
I
I
Oborná
H
H
Fingerová
J
J
Novotný
J
J
Brezinová
L
L
Radová
J
J
Vyslouzilová
J
J
Horáková
J
J
Grohmannová
D000328:Adult; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism; D007248:Infertility, Male / Q000378:metabolism*; D008163:Luminescent Measurements; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D013094:Spermatozoa / Q000378:metabolism*
D017382:Reactive Oxygen Species