Detection of Cryptosporidium molnari oocysts from fish by fluorescent-antibody staining assays for cryptosporidium spp. affecting humans.
Three direct fluorescent-antibody staining assay kits for the detection of zoonotic Cryptosporidium species were used to detect Cryptosporidium molnari from Murray cod, and the cryptosporidia were characterized by using small-subunit (SSU) ribosomal DNA (rDNA). To facilitate rapid diagnosis of infection, this study demonstrated that all three kits detected fresh C. molnari and two kits detected formalin-fixed oocysts.
McMaster Building B14, Faculty of Veterinary Science, University of Sydney, Sydney, New South Wales 2006, Australia.
Rona
R
Barugahare
Michelle M
MM
Dennis
Joy A
JA
Becker
Jan
J
Slapeta
D000818:Animals; D000913:Antibodies, Protozoan; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D019085:Fluorescent Antibody Technique, Direct; D048388:Gadiformes / Q000469:parasitology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D033661:Oocysts / Q000145:classification; D010274:Parasitology / Q000379:methods*; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013194:Staining and Labeling / Q000379:methods
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S
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