Direct genotyping of Toxoplasma gondii in ocular fluid samples from 20 patients with ocular toxoplasmosis: predominance of type II in France.
We report the direct genotyping analysis of Toxoplasma gondii in ocular samples collected from 20 patients, as well as associated clinical and epidemiological data. This work was aimed at better understanding the impact of genotypes of Toxoplasma gondii strains on toxoplasmic retinochoroiditis. For this purpose, we studied the aqueous humor (AH) or vitreous humor (VH) of 20 patients presenting with ocular toxoplasmosis (OT) in 2 hospitals in France. Genetic characterization was obtained with microsatellite markers in a multiplex PCR assay. In contrast to the results of previous studies, we found no association between atypical Toxoplasma gondii genotypes and the occurrence of OT. Considering the local epidemiological data, our OT patients seemed to be infected more frequently by ordinary type II strains found in the environment. In conclusion, direct genotyping of Toxoplasma gondii strains from aqueous or vitreous humor showed a predominance of the type II genotype in ocular toxoplasmosis; this may be due to a high exposure rate of this genotype in humans.
AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Parasitologie-Mycologie, Paris F-75013 France. arnaud.fekkar@psl.aphp.fr
A
A
Fekkar
D
D
Ajzenberg
B
B
Bodaghi
F
F
Touafek
P
P
Le Hoang
J
J
Delmas
P Y
PY
Robert
M L
ML
Dardé
D
D
Mazier
L
L
Paris
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D005260:Female; D005602:France / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014126:Toxoplasmosis, Ocular / Q000469:parasitology*; D014822:Vitreous Body / Q000469:parasitology*; D055815:Young Adult
D016054:DNA, Protozoan
J Clin Microbiol. 2008 Jun;46(6):1965-7
18400917
Am J Ophthalmol. 2003 Dec;136(6):973-88
14644206
J Infect Dis. 2001 Sep 1;184(5):633-9
11474426
Am J Ophthalmol. 2001 Mar;131(3):351-4
11239868
J Clin Microbiol. 2005 Apr;43(4):1940-3
15815026
J Infect Dis. 2009 Apr 15;199(8):1155-67
19265484
Clin Infect Dis. 2007 Oct 1;45(7):e88-95
17806043
J Infect Dis. 2002 Sep 1;186(5):684-9
12195356
Int J Parasitol. 2004 Sep;34(10):1185-96
15380690
Int J Parasitol. 2000 Jan;30(1):69-75
10675747
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14872-7
17804804
Vet Parasitol. 2006 Dec 20;142(3-4):376-9
16919879
Emerg Infect Dis. 2006 Jun;12(6):942-9
16707050
Epidemiol Infect. 1999 Oct;123(2):283-9
10579449
Am J Ophthalmol. 2007 Feb;143(2):354-6
17258534
Am J Ophthalmol. 1992 Aug 15;114(2):136-44
1642287
Am J Ophthalmol. 2005 Feb;139(2):350-1
15734002
Br J Ophthalmol. 2009 Nov;93(11):1546-50
19666926
J Clin Microbiol. 2006 Apr;44(4):1382-9
16597866
Microbes Infect. 2008 Jun;10(7):742-7
18539501
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11423-8
16849431
Vet Parasitol. 2006 Jan 30;135(2):133-6
16188390
J Parasitol. 2010 Aug;96(4):709-12
20486738
Microbes Infect. 2006 Aug;8(9-10):2333-40
16938480
J Clin Microbiol. 2002 Nov;40(11):4037-44
12409371
J Infect Dis. 1995 Dec;172(6):1561-6
7594717
J Clin Microbiol. 2006 Nov;44(11):4262-4
17005742
J Parasitol. 2006 Feb;92(1):36-40
16629312
J Infect. 2006 Aug;53(2):e47-50
16352339
Int J Parasitol. 2010 Feb;40(2):193-200
19631651
J Clin Microbiol. 2000 Nov;38(11):4121-5
11060078