Acetylthiocholine (ATC)--cleaving cholinesterase (ChE) activity as a potential biomarker of pesticide exposure in the Manila clam, Ruditapes philippinarum, of Korea.
The acetylthiocholine (ATC) - cleaving cholinesterase (ChE) activity in Manila clam, which is widely distributed throughout the coastal environment of Korea, was assayed as a potentially useful biomarker of organophosphorous pesticides (OPs). A clear dose-response relationship was determined between inhibited ChE in adductor muscle of clams and four OPs (methidathion, chlorpyrifos, diazinon, IBP) which are heavily used OPs in Korea. The measured EC(50)-24 h values of methidathion, chlorpyrifos, diazinon, and IBP for Ruditapes philippinarum were 7.16 μg l(-1), 0.34 mg l(-1), 3.01 mg l(-1), and 3.41 mg l(-1), respectively. In field studies, ChE activity in Manila clams collected from 23 stations in the mid-western coastal region demonstrated spatial variation with statistical differences. These results suggest that ChE activity in R. philippinarum is a potential biomarker for assessing organophosphorous pesticide contamination in coastal environments.
Marine Environment and Pollution Prevention Research Department, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea.
Jin Young
JY
Choi
Jun
J
Yu
Dong Beom
DB
Yang
Kongtae
K
Ra
Kyung Tae
KT
Kim
Gi Hoon
GH
Hong
Kyung Hoon
KH
Shin
D000122:Acetylthiocholine / Q000378:metabolism*; D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000378:metabolism; D004390:Chlorpyrifos / Q000633:toxicity; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002802:Cholinesterases / Q000378:metabolism*; D003976:Diazinon / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D009946:Organothiophosphorus Compounds / Q000633:toxicity; D010575:Pesticides / Q000633:toxicity*; D056910:Republic of Korea; D014874:Water Pollutants, Chemical / Q000633:toxicity
D002800:Cholinesterase Inhibitors; D009946:Organothiophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical; C009897:O,O-diisopropyl-S-benzylthiophosphate; D000122:Acetylthiocholine; D002802:Cholinesterases; D004390:Chlorpyrifos; C005828:methidathion; D003976:Diazinon