[Effect of assemble flavone of Drynariae Rhizome on activity and calcium deposition of bone marrow stem cells of rabbit].
OBJECTIVE
To observe the effect of assemble flavone of Drynariae Rhizome on the activity and osteogenic differentiation of bone marrow stem cells of rabbit in vitro.


METHODS
Density gradient centrifugation was used to isolate bone marrow stem cells from SD rats. The MSCs were also undergone osteogenic induction. Cytochemical and immunocytochemical tainting were performed to verify their multipotential.


RESULTS
High purity of bone marrow stem cells was successfully obtained. Both ALP activity and mineralization nodes tainting were positive in MSCs after osteogenic differentiation.


CONCLUSIONS
The MSCs sustained their osteogenic differentiation potential in vitro by assemble flavone of rhizome drynaria.
Fifth Affiliated Hospital of Sun Yetsen University, Zhuhai 519000, China. zhshuxc@163.com
Xiaochun
X
Shu
Xiangyang
X
Hu
Lijuan
L
Zhang
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D047309:Flavones / Q000494:pharmacology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029621:Polypodiaceae / Q000737:chemistry*; D011817:Rabbits
D047309:Flavones; D000469:Alkaline Phosphatase; D002118:Calcium