Induction of a Th17 cell response by Helicobacter pylori Urease subunit B.
Th17 cells represent a novel subset of CD4(+) T cells, which is associated with Helicobacter pylori infection. In the present study, we investigated the potential role of Urease subunit B (UreB) in the induction of Th17 cell response. Co-cultured splenic lymphocytes from H. pylori-infected mice with the recombinant UreB (rUreB) elevated IL-17 secretion and caused an increase in the number of Th17 cells. The expression of IL-6 and IL-23 p19 was significantly increased in rUreB-stimulated macrophages. Whole cell protein (WCP) of UreB-deficient strain (UreB(-) strain) induced less Th17 cell responses than that of wild-type strain. In addition, subcutaneous and intranasal immunization of rUreB elicited antigen-specific Th17 cell responses. Intranasal immunization of rUreB reduced H. pylori colonization in the stomach, which was closely related with the increased rUreB-specific Th17 cell responses. These results suggest that UreB is an important protein which is able to elicit Th17 cell responses against H. pylori both in vivo and in vitro.
Department of Clinical Microbiology and Immunology, College of Medical Laboratory, The Third Military Medical University, Chongqing, PR China.
Jin-Yu
JY
Zhang
Tao
T
Liu
Hong
H
Guo
Xiao-Fei
XF
Liu
Yuan
Y
Zhuang
Shu
S
Yu
Li
L
Chen
Chao
C
Wu
Zhuo
Z
Zhao
Bin
B
Tang
Ping
P
Luo
Xu-Hu
XH
Mao
Gang
G
Guo
Yun
Y
Shi
Quan-Ming
QM
Zou
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D008213:Lymphocyte Activation / Q000235:genetics; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation / Q000235:genetics; D021122:Protein Subunits / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013270:Stomach / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D014510:Urease / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D001426:Bacterial Proteins; D016207:Cytokines; D021122:Protein Subunits; D011994:Recombinant Proteins; D014510:Urease