Micrococcus lactis sp. nov., isolated from dairy industry waste.
A Gram-positive, yellow-pigmented, actinobacterial strain, DW152(T), was isolated from a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis indicated that strain DW152(T) exhibited low similarity with many species with validly published names belonging to the genera Micrococcus and Arthrobacter. However, phenotypic properties including chemotaxonomic markers affiliated strain DW152(T) to the genus Micrococcus. Strain DW152(T) had ai-C(15:0) and i-C(15:0) as major cellular fatty acids, and MK-8(H(2)) as the major menaquinone. The cell-wall peptidoglycan of strain DW152(T) had l-lysine as the diagnostic amino acid and the type was A4α. The DNA G+C content of strain DW152(T) was 68.0 mol%. In 16S rRNA gene sequence analysis, strain DW152(T) exhibited significant similarity with Micrococcus terreus NBRC 104258(T), but the mean value of DNA-DNA relatedness between these strains was only 42.3%. Moreover, strain DW152(T) differed in biochemical and chemotaxonomic characteristics from M. terreus and other species of the genus Micrococcus. Based on the above differences, we conclude that strain DW152(T) should be treated as a novel species of the genus Micrococcus, for which the name Micrococcus lactis sp. nov. is proposed. The type strain of Micrococcus lactis sp. nov. is DW152(T) (=MTCC10523(T) =DSM 23694(T)).
Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Chittpurna
Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Pradip K
PK
Singh
Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Dipti
D
Verma
Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Anil Kumar
AK
Pinnaka
Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Shanmugam
S
Mayilraj
Microbial Type Culture Collection and Gene bank, Institute of Microbial Technology, Sector 39A, Chandigarh - 160 036, India.
Suresh
S
Korpole
D000818:Animals; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology; D005227:Fatty Acids / Q000378:metabolism; D019649:Food Industry; D008837:Micrococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage