Effects of nanofiber/stem cell composite on wound healing in acute full-thickness skin wounds.
Acute full-thickness skin wounds (FTSW) caused by extensive burns or high-energy trauma are not adequately addressed by current clinical treatments. This study hypothesized that biomimetic nanofiber scaffolds (NFSs) functionalized with rich attachment of bone-marrow-derived mesenchymal stem cells (BM-MSCs) can promote wound healing in acute FTSW. Results in a rat model showed that both NFS and BM-MSCs contributed to the wound healing. Wounds in NFS group with a higher density of BM-MSCs achieved complete closure 8 days earlier than the control group. Implanted BM-MSCs were found to promote epithelial edge ingrowth and collagen synthesis. The colocation of BM-MSCs (tagged with quantum-dots) with the expression of keratin 10 and filaggrin indicated the participation of BM-MSCs in epidermal differentiation at early and intermediate stages under the local wounding environment. Overall, this study suggests a great potential of using NFS/BM-MSC composites for the treatment of acute FTSW.
NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, Singapore, Singapore. makun041210@gmail.com
Kun
K
Ma
Susan
S
Liao
Liumin
L
He
Jia
J
Lu
Seeram
S
Ramakrishna
Casey K
CK
Chan
D000818:Animals; D040761:Biomimetic Materials; D001854:Bone Marrow Cells / Q000378:metabolism*; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D002454:Cell Differentiation; D000091344:Filaggrin Proteins; D006801:Humans; D007381:Intermediate Filament Proteins; D053550:Keratin-10; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D057139:Nanofibers; D045663:Quantum Dots; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D014183:Transplantation, Heterologous; D014945:Wound Healing
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D053550:Keratin-10