Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli.
Phage shock proteins (Psp) and their homologues are found in species from the three domains of life: Bacteria, Archaea and Eukarya (e.g. higher plants). In enterobacteria, the Psp response helps to maintain the proton motive force (PMF) of the cell when the inner membrane integrity is impaired. The presumed ability of ArcB to sense redox changes in the cellular quinone pool and the strong decrease of psp induction in ΔubiG or ΔarcAB backgrounds suggest a link between the Psp response and the quinone pool. The authors now provide evidence indicating that the physiological signal for inducing psp by secretin-induced stress is neither the quinone redox state nor a drop in PMF. Neither the loss of the H(+)-gradient nor the dissipation of the electrical potential alone is sufficient to induce the Psp response. A set of electron transport mutants differing in their redox states due to the lack of a NADH dehydrogenase and a quinol oxidase, but retaining a normal PMF displayed low levels of psp induction inversely related to oxidised ubiquinone levels under microaerobic growth and independent of PMF. In contrast, cells displaying higher secretin induced psp expression showed increased levels of ubiquinone. Taken together, this study suggests that not a single but likely multiple signals are needed to be integrated to induce the Psp response.
Skirball Institute of Biomolecular Medicine, New York University Medical School, New York, USA. Christoph.Engl@med.nyu.edu
Christoph
C
Engl
Alex Ter
AT
Beek
Martijn
M
Bekker
Joost Teixeira
JT
de Mattos
Goran
G
Jovanovic
Martin
M
Buck
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D017101:Bacteriophage P1 / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018892:Proton-Motive Force; D014451:Ubiquinone / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C069816:phage shock protein, Bacteria; D014451:Ubiquinone
J Mol Biol. 2009 Dec 11;394(4):764-75
19804784
J Bacteriol. 1989 Feb;171(2):868-73
2644240
Mol Syst Biol. 2006;2:2006.0008
16738554
Mol Microbiol. 2009 Oct;74(1):16-28
19682256
Arch Microbiol. 1988;150(5):499-503
2849923
J Bacteriol. 2010 Apr;192(8):2193-209
20061485
J Biol Chem. 2006 Jul 28;281(30):21147-21161
16709570
Adv Exp Med Biol. 2007;603:167-77
17966413
J Mol Biol. 2002 Jun 28;320(1):23-37
12079332
J Biol Chem. 2004 Dec 31;279(53):55707-14
15485810
Nature. 2008 Jan 24;451(7177):489-92
18216859
Mol Microbiol. 2005 Aug;57(3):621-8
16045608
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4243-8
11274448
Nat Rev Microbiol. 2006 May;4(5):383-94
16715050
J Bacteriol. 2010 Feb;192(3):746-54
19933363
Mol Microbiol. 1997 Apr;24(2):255-61
9159513
Science. 2001 Jun 22;292(5525):2314-6
11423658
Proc Natl Acad Sci U S A. 1990 Feb;87(3):862-6
2105503
J Bacteriol. 2008 May;190(10):3475-81
18326578
Proc Natl Acad Sci U S A. 1988 Mar;85(6):1888-92
2964639
Microbiology (Reading). 2008 May;154(Pt 5):1436-1443
18451052
Mol Microbiol. 2008 May;68(3):538-46
18331472
DNA Res. 2005;12(5):291-9
16769691
Microbiology (Reading). 2007 Jun;153(Pt 6):1974-1980
17526854
J Mol Biol. 1997 Oct 24;273(2):377-88
9344746
Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13318-23
15326287
J Bacteriol. 2003 Nov;185(22):6707-11
14594846
Mol Microbiol. 2009 Aug;73(3):382-96
19555453
J Bacteriol. 2000 Aug;182(15):4129-36
10894718
Mol Microbiol. 2010 Aug;77(3):743-54
20545840
Microbiology (Reading). 2006 Aug;152(Pt 8):2197-2205
16849787
Mol Microbiol. 2005 May;56(4):1004-16
15853886
EMBO J. 1996 Jan 2;15(1):162-71
8598199
Mol Microbiol. 2004 Feb;51(3):659-74
14731270
J Bacteriol. 1998 May;180(10):2782-7
9573170
Mol Microbiol. 2006 Nov;62(3):823-37
16995896
J Bacteriol. 2000 Jan;182(2):311-9
10629175
J Bacteriol. 1997 Sep;179(17):5429-35
9286997
Mol Microbiol. 2003 Jan;47(1):103-18
12492857
Science. 2005 Mar 25;307(5717):1972-5
15790859
Microbiology (Reading). 1999 Aug;145 ( Pt 8):1817-1830
10463148
Microbiology (Reading). 2010 Oct;156(Pt 10):2920-2932
20595257
Mol Microbiol. 2007 Oct;66(1):100-9
17725563
Biochim Biophys Acta. 1977 Jul 7;461(1):84-100
195602
Arch Microbiol. 1992;158(1):68-73
1444716
FEMS Microbiol Rev. 2010 Sep;34(5):797-827
20636484