Identification of Plesiomonas spp.: serological and MALDI-TOF MS methods.
Biochemical and serological profiles of isolates of Plesiomonas shigelloides were assayed using standard procedures in isolates from various clinical samples. Seventy-four isolates, including P. shigelloides type strain, were further characterized by MALDI-TOF MS using 3-methoxy-4-hydroxycinnamic acid as matrix. Multiple ions in the 3- to 12-kDa mass range were found in the spectra of each strain, from which the "species-identifying" unique biomarker ions were identified. After creating the species-specific patterns, a spectral database was generated for reliable, rapid, reproducible and accurate identification of Plesiomonas strains. The classical strain description (biochemical and serological) was thus complemented with the metabolic (proteomic) characterization.
Czech National Collection of Type Cultures (CNCTC), National Institute of Public Health, 10042 Prague, Czech Republic. rkolinska@szu.cz
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R
Kolínská
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Dřevínek
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E
Aldová
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H
Zemličková
D001431:Bacteriological Techniques / Q000379:methods*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D016981:Plesiomonas / Q000737:chemistry / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012703:Serotyping / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
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