Leishmania (Viannia) braziliensis identification by PCR in the state of Para, Brazil.
The incidence of cutaneous leishmaniasis (CL) is increasing and there is limited surveillance of Leishmania species throughout the world. We identified the species associated with CL in a region of Amazonia, an area recognized for its Leishmania species variability. Clinical findings were analyzed and correlated with the species identified in 93 patients. PCR assays were based on small subunit ribosomal DNA (SSU-rDNA) and G6PD, and were performed in a laboratory located 3,500km away. Leishmania (V.) braziliensis was identified in 53 patients (57%). The other 40 patients (43%) carried a different species (including six cases of L. (L.) amazonensis). Molecular methods can be employed, using special media, to allow transport to distant laboratories. L. (V.) braziliensis is the most common species in the area of Para. The location of ulcers can suggest CL species.
Department of Infectious Diseases, University of São Paulo, Medical School, São Paulo, Brazil. hbacha@terra.com.br
H A
HA
Bacha
F F
FF
Tuon
R A
RA
Zampieri
L M
LM
Floeter-Winter
J
J
Oliveira
A C
AC
Nicodemo
M M
MM
Quiroga
M
M
Mascheretti
M
M
Boulos
V S
VS
Amato
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D004197:Disease Reservoirs; D005260:Female; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D007892:Leishmania braziliensis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000145:classification / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity; D055815:Young Adult