Lipopolysaccharide-stimulated activation of murine DC2.4 cells is attenuated by n-butylidenephthalide through suppression of the NF-κB pathway.
Modulation of dendritic cell (DC) fate and function may be one approach for the treatment of inflammatory and autoimmune diseases. n-Butylidenephthalide (BP), derived from Angelica sinensis, at 40 μg/ml significantly decreased the secretion of interleukin-6 and tumor necrosis factor-α by lipopolysaccharide (LPS)-stimulated activation of cultured murine DC2.4 cells (P<0.01). LPS-induced major histocompatibility complex class II (P<0.05), CD86 (P<0.01) and CD40 (P<0.01) expression on DC2.4 cells was also inhibited by BP. The endocytic capacity of LPS-stimulated DC2.4 cells was increased by BP (P<0.01). The antigen-presenting capacity of LPS-stimulated DC2.4 cells was decreased by BP (P<0.05). Moreover, we confirmed BP attenuates the responses of LPS-stimulated activation of DCs via suppression of NF-κB-dependent pathways.
Graduate Institute of Immunology, China Medical University, Taichung 40402, Taiwan. rhfu@mail.cmu.edu.tw
Ru-Huei
RH
Fu
Horng-Jyh
HJ
Hran
Ching-Liang
CL
Chu
Chin-Mao
CM
Huang
Shih-Ping
SP
Liu
Yu-Chi
YC
Wang
Ya-Hsien
YH
Lin
Woei-Cherng
WC
Shyu
Shinn-Zong
SZ
Lin
D029971:Angelica sinensis / Q000737:chemistry; D000818:Animals; D051940:B7-2 Antigen / Q000096:biosynthesis; D019013:CD40 Antigens / Q000096:biosynthesis; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D010796:Phthalic Anhydrides / Q000302:isolation & purification / Q000378:metabolism*
D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010796:Phthalic Anhydrides; C026105:butylidenephthalide