FGF19 protects colonic epithelial cells against hydrogen peroxide.
OBJECTIVE
The apoptosis induced by hydrogen peroxide (H₂O₂) in colonic epithelial cells is very important for the pathogenesis of inflammatory bowel disease (IBD). Fibroblast growth factor (FGF) 15, the human ortholog of FGF19, is reported to be secreted from colonic myofibroblasts and enhances colonic epithelial restitution, but little is known about the function of FGF19 to colonic epithelial cells. In the present study, we investigate the anti-apoptosis effect of FGF19 in colonic epithelial cells treated with H₂O₂.


METHODS
Young adult mouse colonic epithelial (YAMC) cells are used to investigate the protective role of FGF19. Cellular viability was determined by WST-8 assay, and apoptosis was measured by Hoechst staining and Western blotting of cleaved caspase-3. YAMC cells were pretreated by FGF19 and H₂O₂ was used for cellular damage.


RESULTS
We demonstrated that pretreatment of FGF19 (50 ng/ml) significantly protects YAMC cells treated with H₂O₂ assessed by WST-8. We also demonstrated Hoechst staining of YAMC cells and that H₂O₂-induced apoptosis is significantly reduced by FGF19 treatment via inhibition of the caspase-3 pathway.


CONCLUSIONS
These results indicate FGF19 protects YAMC cells against H₂O₂ and might be related to the pathogenesis of IBD. Even further studies are needed - FGF19 may be one of the possible therapeutic strategies of IBD.
Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan.
Kazuhiko
K
Uchiyama
Yuji
Y
Naito
Tomohisa
T
Takagi
Katsura
K
Mizushima
Natsuko
N
Hayashi
Osamu
O
Handa
Takeshi
T
Ishikawa
Nobuaki
N
Yagi
Satoshi
S
Kokura
Toshikazu
T
Yoshikawa
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects; D005346:Fibroblast Growth Factors / Q000494:pharmacology* / Q000502:physiology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000503:physiopathology; D051379:Mice
C108526:fibroblast growth factor 15, mouse; D005346:Fibroblast Growth Factors; D006861:Hydrogen Peroxide