title: Magnetosome-like ferrimagnetic iron oxide nanocubes for highly sensitive MRI of single cells and transplanted pancreatic islets.
abstract: For ultrasensitive magnetic resonance imaging (MRI), magnetic nanoparticles with extremely high r2 relaxivity are strongly desired. Magnetosome-like nanoparticles were prepared by coating polyethylene glycol-phospholipid (PEG-phospholipid) onto ferrimagnetic iron oxide nanocubes (FIONs). FIONs exhibited a very high relaxivity (r2) of 324 mM(-1) s(-1), allowing efficient labeling of various kinds of cells. The magnetic resonance (MR) imaging of single cells labeled with FIONs is demonstrated not only in vitro but also in vivo. Pancreatic islet grafts and their rejection could be imaged using FIONs on a 1.5 T clinical MRI scanner. The strong contrast effect of FIONs enabled MR imaging of transplanted islets in small rodents as well as in large animals. Therefore, we expect that MR imaging of pancreatic islet grafts using FIONs has the potentials for clinical applications. Furthermore, FIONs will enable highly sensitive noninvasive assessment after cell transplantation.
authors.affiliation: National Creative Research Initiative Center for Oxide Nanocrystalline Materials, World Class University Program of Chemical Convergence for Energy and Environment, Seoul National University, Seoul 151-744, Korea.
authors.forename: Nohyun
authors.initials: N
authors.lastname: Lee
authors.forename: Hyoungsu
authors.initials: H
authors.lastname: Kim
authors.forename: Seung Hong
authors.initials: SH
authors.lastname: Choi
authors.forename: Mihyun
authors.initials: M
authors.lastname: Park
authors.forename: Dokyoon
authors.initials: D
authors.lastname: Kim
authors.forename: Hyo-Cheol
authors.initials: HC
authors.lastname: Kim
authors.forename: Yoonseok
authors.initials: Y
authors.lastname: Choi
authors.forename: Shunmei
authors.initials: S
authors.lastname: Lin
authors.forename: Byung Hyo
authors.initials: BH
authors.lastname: Kim
authors.forename: Hye Seung
authors.initials: HS
authors.lastname: Jung
authors.forename: Hyeonjin
authors.initials: H
authors.lastname: Kim
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Moon
authors.forename: Taeghwan
authors.initials: T
authors.lastname: Hyeon
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry; D007515:Islets of Langerhans / Q000648:ultrastructure*; D016381:Islets of Langerhans Transplantation / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008991:Monitoring, Physiologic / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010743:Phospholipids; C000499:ferric oxide; D011092:Polyethylene Glycols