title: Synthesis of O-oleoyl-chitosan and its sorption properties for lipoproteins.
abstract: In this study a lipotropic chitosan derivative, O-oleoyl-chitosan (OOCs), was obtained through the attachment of oleoyl groups to hydroxyl groups at C-6 of chitosan. The degree of substitution of OOCs was approx. 14-15%. OOCs had good solubility in organic solvents. Lipoprotein  sorption tests in vitro and circular dichroism spectroscopy were applied to investigate the interaction between lipoproteins and OOCs. Observations indicated that the lipotropic OOCs, especially with lower viscosity-average molecular weight, could improve both LDL binding capacity and  selectivity in human plasma. Circular dichroism analysis showed that the secondary structure of LDL was markedly changed by OOCs, for the α-helical content of the LDL was increased when interacted with OOCs. OOCs were proven to be effective for selective removal of LDL in plasma.
authors.affiliation: Department of Polymer Materials Science and Engineering, College of Materials Science and Engineering, Tianjin University, Tianjin 300072, P. R. China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Liu
authors.forename: Anrong
authors.initials: A
authors.lastname: Zeng
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D000924:Anticholesteremic Agents / Q000138:chemical synthesis* / Q000737:chemistry*; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D019301:Oleic Acid / Q000737:chemistry*; D012995:Solubility; D014783:Viscosity
chemical_list: D000924:Anticholesteremic Agents; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D019301:Oleic Acid; D048271:Chitosan