title: Bacillus Calmette-Guérin vaccination using a microneedle patch.
abstract: Tuberculosis (TB) caused by Mycobacterium tuberculosis continues to be a leading cause of mortality among bacterial diseases, and the bacillus Calmette-Guérin (BCG) is the only licensed vaccine for human use against this disease. TB prevention and control would benefit from an improved method of BCG vaccination that simplifies logistics and eliminates dangers posed by hypodermic needles without compromising immunogenicity. Here, we report the design and engineering of a BCG-coated microneedle vaccine patch for a simple and improved intradermal delivery of the vaccine. The microneedle vaccine patch induced a robust cell-mediated immune response in both the lungs and the spleen of guinea pigs. The response was comparable to the traditional hypodermic needle based intradermal BCG vaccination and was characterized by a strong antigen specific lymphocyte proliferation and IFN-γ levels with high frequencies of CD4(+)IFN-γ(+), CD4(+)TNF-α(+) and CD4(+)IFN-γ(+)TNF-α(+) T cells. The BCG-coated microneedle vaccine patch was highly immunogenic in guinea pigs and supports further exploration of this new technology as a simpler, safer, and compliant vaccination that could facilitate increased coverage, especially in developing countries that lack adequate healthcare infrastructure.
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, N.W., Atlanta, GA 30332, USA.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hiraishi
authors.forename: Subhadra
authors.initials: S
authors.lastname: Nandakumar
authors.forename: Seong-O
authors.initials: SO
authors.lastname: Choi
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Yeu-Chun
authors.initials: YC
authors.lastname: Kim
authors.forename: James E
authors.initials: JE
authors.lastname: Posey
authors.forename: Suraj B
authors.initials: SB
authors.lastname: Sable
authors.forename: Mark R
authors.initials: MR
authors.lastname: Prausnitz
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001500:BCG Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D005260:Female; D006168:Guinea Pigs; D007371:Interferon-gamma / Q000096:biosynthesis; D008168:Lung / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014611:Vaccination / Q000295:instrumentation* / Q000379:methods*
chemical_list: D001500:BCG Vaccine; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma