title: Human immune responses to Burkholderia pseudomallei characterized by protein microarray analysis.
abstract: BACKGROUND
  We aimed to determine the antibody and T cell responses to Burkholderia pseudomallei of humans to select candidate vaccine antigens.
  
  
  METHODS
  For antibody profiling, a protein microarray of 154 B. pseudomallei proteins was probed with plasma from 108 healthy individuals and 72 recovered patients. Blood from 20 of the healthy and 30 of the recovered individuals was also obtained for T cell assays.
  
  
  RESULTS
  Twenty-seven proteins distinctively reacted with human plasma following environmental exposure or clinical melioidosis. We compared the responses according to the patient's history of subsequent relapse, and antibody response to BPSL2765 was higher in plasma from individuals who had only 1 episode of disease than in those with recurrent melioidosis. A comparison of antibody and T cell responses to 5 B. pseudomallei proteins revealed that BimA and flagellin-induced responses were similar but that BPSS0530 could induce T cell responses in healthy controls more than in recovered patients.
  
  
  CONCLUSIONS
  By combining large-scale antibody microarrays and assays of T cell-mediated immunity, we identified a panel of novel B. pseudomallei proteins that show distinct patterns of reactivity in different stages of human melioidosis. These proteins may be useful candidates for development of subunit-based vaccines and in monitoring the risks of treatment failure and relapse.
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen,Thailand.
authors.forename: Duangchan
authors.initials: D
authors.lastname: Suwannasaen
authors.forename: Jirawan
authors.initials: J
authors.lastname: Mahawantung
authors.forename: Wipada
authors.initials: W
authors.lastname: Chaowagul
authors.forename: Direk
authors.initials: D
authors.lastname: Limmathurotsakul
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.forename: Huw
authors.initials: H
authors.lastname: Davies
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Bancroft
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
authors.forename: Ganjana
authors.initials: G
authors.lastname: Lertmemongkolchai
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D008554:Melioidosis / Q000276:immunology*; D040081:Protein Array Analysis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial