title: Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.
abstract: A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.
authors.affiliation: Center of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Dawei
authors.initials: D
authors.lastname: Cui
authors.forename: Shufa
authors.initials: S
authors.lastname: Zheng
authors.forename: Shigui
authors.initials: S
authors.lastname: Yang
authors.forename: Jia
authors.initials: J
authors.lastname: Tong
authors.forename: Dagan
authors.initials: D
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Fan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Lou
authors.forename: Xuefen
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhuge
authors.forename: Bo
authors.initials: B
authors.lastname: Ye
authors.forename: Baode
authors.initials: B
authors.lastname: Chen
authors.forename: Weilin
authors.initials: W
authors.lastname: Mao
authors.forename: Yajun
authors.initials: Y
authors.lastname: Tan
authors.forename: Genyun
authors.initials: G
authors.lastname: Xu
authors.forename: Zhenjin
authors.initials: Z
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000302:isolation & purification; D009981:Influenza B virus / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*