title: Ternary copper(II) complexes of levofloxacin and phenanthroline derivatives: in-vitro antibacterial, DNA interactions, and SOD-like activity.
abstract: A series of ternary copper(II) complexes have been derived using levofloxacin and five phenanthroline derivatives. Complexes were characterized using infrared spectroscopy, Thermogravimetric (TG)-analysis, fast atom bombardment mass spectroscopy and reflectance spectra. Synthesized complexes exhibit the only d-d band at ∼ 666 nm points toward a distorted square pyramidal geometry at metal centre with one unpaired electron responsible for paramagnetic behaviour of whole moiety. Binding behaviour of the complexes toward Herring Sperm DNA were determined using ultraviolet-Vis (UV-Vis) absorption titration and viscometric titration experiment, where as the cleavage efficacy of the complexes toward pUC19 DNA was determined by electrophoresis in presence of ethidium bromide. Complexes exhibit superoxide dismutase-like activity with their IC(50) values ranging from 0.7917 to 1.7432 µM.
authors.affiliation: Department of Chemistry, Sardar Patel University, Anand, Gujarat, India. jeenenpatel@yahoo.co.in
authors.forename: M N
authors.initials: MN
authors.lastname: Patel
authors.forename: Pradhuman Amrutsinh
authors.initials: PA
authors.lastname: Parmar
authors.forename: Deepen Sureshbhai
authors.initials: DS
authors.lastname: Gandhi
authors.forename: Vasudev R
authors.initials: VR
authors.lastname: Thakkar
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D053837:DNA Cleavage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D064704:Levofloxacin; D015242:Ofloxacin / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010618:Phenanthrolines / Q000737:chemistry; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D009942:Organometallic Compounds; D010618:Phenanthrolines; D064704:Levofloxacin; D003300:Copper; D004247:DNA; D015242:Ofloxacin; D013482:Superoxide Dismutase