title: Free calvarial periosteum graft vascularized by an omental flap in a rat model.
abstract: Because omental flaps are useful for flap prefabrication and the cambium layer of the periosteum can be osteogenic, we examined whether calvarial periosteum grafted onto greater omentum of rats was osteogenic and suitable for a flap. Distal omentum was wrapped with calvarial periosteum and so the cambium faced the omentum. Grafted omentum was harvested at 1 to 9 days. In other rats, grafted omentum was elevated as a pedicled flap and moved to the abdominal subcutis, to be harvested later at 1 to 5 months after the initial surgery. Bone formation was evaluated histologically, histochemically, and radiographically. On day 3, osteoid had formed. From day 4, calvarial periosteum was revascularized by omentum and bone was forming. New bone was maintained after grafting to subcutis for 5 months. Thus, bone formed by periosteum on the omentum could be used to reconstruct defects of the bone.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan. kosuke7714@ybb.ne.jp
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nakazato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Toriyama
authors.forename: Masashi
authors.initials: M
authors.lastname: Hishida
authors.forename: Keisuke
authors.initials: K
authors.lastname: Takanari
authors.forename: Shunjiro
authors.initials: S
authors.lastname: Yagi
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Shinya
authors.initials: S
authors.lastname: Akatsuka
authors.forename: Akemi
authors.initials: A
authors.lastname: Hayakawa
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kamei
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D007124:Immunoenzyme Techniques; D008297:Male; D023421:Models, Animal; D009852:Omentum / Q000098:blood supply* / Q000637:transplantation*; D010012:Osteogenesis; D010521:Periosteum / Q000098:blood supply* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013524:Surgical Flaps / Q000098:blood supply*; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D014841:von Willebrand Factor; D000469:Alkaline Phosphatase