title: Emw1p/YNL313cp is essential for maintenance of the cell wall in Saccharomyces cerevisiae.
abstract: There are six essential genes in the Saccharomyces cerevisiae genome which encode proteins bearing the tetratricopeptide repeat (TPR) domain that mediates protein-protein interaction. Thus far, the function of one of them, YNL313c, remains unknown. Our conditional mutants of YNL313c display osmoremedial temperature sensitivity, hypersensitivity to both Calcofluor White and low concentrations of SDS, and osmoremedial caffeine sensitivity. These are hallmarks of mutants that display cell wall defects. Accordingly we rename the gene as EMW1 (essential for maintenance of the cell wall). Loss of Emw1p function is not associated with abrogation of the cell wall integrity (CWI) MAP kinase cascade. Instead, emw1(ts) mutants activate this cascade even at permissive temperature, indicating that loss of Emw1p function does not cause a defect in sensors and effectors of cell wall signalling, but leads to a cell wall defect directly. Constitutive activation of the CWI cascade is reflected by the overproduction of chitin by emw1(ts) mutants, a compensatory response frequently displayed by cell wall mutants. Growth is restored to emw1(ts) mutants incubated at otherwise non-permissive temperature when GFA1 is overexpressed. GFA1 encodes the hexosephosphate aminotransferase that catalyses the rate-limiting step in the pathway that synthesizes the chitin precursor UDP-GlcNAc. The possibility that Emw1p is required for function of Gfa1p was ruled out, because the emw1(ts) phenotype persists when the requirement for Gfa1p is bypassed. Furthermore, if loss of Emw1p function leads to loss of function of Gfa1p, then chitin synthesis would be diminished. Instead, a stimulation of the synthesis of this polymer is detected. Consequently, the defect associated with emw1(ts) mutants may be associated with compromise in one of the remaining processes that depend on UDP-GlcNAc, namely N-glycosylation or glycosylphosphatidylinositol (GPI)-anchor synthesis.
authors.affiliation: Pharmaceutical Science Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Sipling
authors.affiliation: Pharmaceutical Science Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhai
authors.affiliation: Pharmaceutical Science Division, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
authors.forename: Barry
authors.initials: B
authors.lastname: Panaretou
mesh_terms: D000935:Antifungal Agents / Q000633:toxicity; D001557:Benzenesulfonates / Q000633:toxicity; D002110:Caffeine / Q000633:toxicity; D002473:Cell Wall / Q000378:metabolism / Q000502:physiology*; D002686:Chitin / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D001557:Benzenesulfonates; C568826:Emw1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D002686:Chitin; D012967:Sodium Dodecyl Sulfate; D002110:Caffeine; C007061:C.I. Fluorescent Brightening Agent 28; C098848:GFA1 protein, S cerevisiae; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)