title: Modulation of cytokine and chemokine secretions in rhesus monkey trophoblast co-culture with decidual but not peripheral blood monocyte-derived macrophages.
abstract: OBJECTIVE
  Decidual macrophages are thought to promote pregnancy success, in part through interactions with invading trophoblast cells in hemochorial placentation. However, the factors that constitute this regulatory cross talk are not well understood.
  
  
  METHODS
  Rhesus monkey decidual and peripheral blood-derived macrophages were co-cultured with primary Rhesus trophoblasts. Macrophage functions including cell-surface marker expression, antigen uptake and processing, in vitro migration, and cytokine and chemokine secretions were evaluated.
  
  
  RESULTS
  While most macrophage functions were unchanged by trophoblast co-culture, changes in the secretion of selected cytokines and the migration of trophoblasts were noted when decidual (but generally, not peripheral blood monocyte-derived) macrophages were cultured with trophoblasts. In addition, basal secretion differed significantly between peripheral blood-derived and decidual macrophages for a broad spectrum of cytokines. When trophoblasts were pre-treated with an anti-Mamu-AG antibody, 25D3, there was no change in cytokine or chemokine secretion.
  
  
  CONCLUSIONS
  Macrophage cytokine expression can be modulated by trophoblast co-culture, but it remains unclear how Mamu-AG is involved.
authors.affiliation: Department of Comparative Biosciences, University of Wisconsin-Madison, 1223 Capitol Court, Madison,WI 53715-1299, USA. annbaker@wisc.edu
authors.forename: Ann E
authors.initials: AE
authors.lastname: Rozner
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Dambaeva
authors.forename: Jessica G
authors.initials: JG
authors.lastname: Drenzek
authors.forename: Maureen
authors.initials: M
authors.lastname: Durning
authors.forename: Thaddeus G
authors.initials: TG
authors.lastname: Golos
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018925:Chemokines / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D018920:Coculture Techniques; D016207:Cytokines / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D003656:Decidua / Q000166:cytology* / Q000276:immunology; D005260:Female; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008253:Macaca mulatta; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D011247:Pregnancy; D011270:Pregnancy, Animal; D014327:Trophoblasts / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
chemical_list: D018925:Chemokines; D016207:Cytokines