title: Evaluation of the immunogenicity of a transgenic tobacco plant expressing the recombinant fusion protein of GP5 of porcine reproductive and respiratory syndrome virus and B subunit of Escherichia coli heat-labile enterotoxin in pigs.
abstract: Escherichia coli heat-labile enterotoxin B subunit (LTB) can be used as an adjuvant for co-administered antigens. Our previous study showed that the expression of neutralizing epitope GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) in transgenic tobacco plant (GP5-T) could induce PRRSV-specific immune responses in pigs. A transgenic tobacco plant co-expressing LTB and PRRSV GP5 as a fusion protein (LTB-GP5-T) was further constructed and its immunogenicity was evaluated. Pigs were given orally three consecutive doses of equal concentration of recombinant GP5 protein expressed in leaves of LTB-GP5-T or GP5-T at a 2-week interval and challenged with PRRSV at 7 weeks post-initial immunization. Pigs receiving LTB-GP5-T or GP5-T developed PRRSV-specific antibody- and cell-mediated immunity and showed significantly lower viremia and tissue viral load and milder lung lesions than wild type tobacco plant (W-T). The LTB-GP5-T-treated group had relatively higher immune responses than the GP5-T-treated group, although the differences were not statistically significant.
authors.affiliation: Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, Taipei 106, Taiwan, ROC.
authors.forename: Min-Yuan
authors.initials: MY
authors.lastname: Chia
authors.forename: Shih-Hsuan
authors.initials: SH
authors.lastname: Hsiao
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Chan
authors.forename: Yi-Yin
authors.initials: YY
authors.lastname: Do
authors.forename: Pung-Ling
authors.initials: PL
authors.lastname: Huang
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Chang
authors.forename: Yi-Chieh
authors.initials: YC
authors.lastname: Tsai
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Lin
authors.forename: Victor Fei
authors.initials: VF
authors.lastname: Pang
authors.forename: Chian-Ren
authors.initials: CR
authors.lastname: Jeng
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000378:metabolism; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004768:Enterotoxins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D029968:Escherichia coli Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D018515:Plant Leaves / Q000276:immunology; D030821:Plants, Genetically Modified; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012463:Saliva / Q000276:immunology; D034421:Sus scrofa; D013552:Swine; D014026:Nicotiana / Q000235:genetics* / Q000276:immunology*; D022462:Vaccines, Edible / Q000008:administration & dosage / Q000235:genetics; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D019562:Viral Load; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D001427:Bacterial Toxins; D017931:DNA Primers; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D022462:Vaccines, Edible; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV; C027538:heat-labile enterotoxin, E coli