title: Improved method for rapid and efficient determination of genome replication and protein expression of clinical hepatitis B virus isolates.
abstract: Different hepatitis B virus (HBV) genotypes and variants are associated with different clinical outcomes and/or response to antiviral therapy, yet the comparison of the in vitro replication capacity of a large number of clinical isolates remains technically challenging and time-consuming. Although the full-length HBV genome can be amplified from high-titer blood samples by PCR using High Fidelity(plus) DNA polymerase and primers targeting the conserved precore region, the HBV clones thus generated are replication deficient due to the inability to generate the terminally redundant pregenomic RNA essential for genome replication. The transfection experiment is further complicated by PCR errors and the presence of viral quasispecies. A previous study found that the precise removal of non-HBV sequence by SapI digestion led to HBV replication in transfected cells, possibly due to low-level genome circularization by a cellular enzyme. We released HBV genome from the cloning vector using BspQI, an inexpensive isoschizomer of SapI, and increased the efficiency of genome replication by an extra step of in vitro DNA ligation. The uncut plasmid DNA can be used for transfection if the sole purpose is to study envelope protein expression. We found significant PCR errors associated with the High Fidelity(plus) DNA polymerase, which could be greatly diminished using Phusion DNA polymerase or masked by the use of a clone pool. The reduced PCR error and modified enzymatic steps prior to transfection should facilitate a more widespread functional characterization of clinical HBV isolates, while the clone pool approach is useful for samples with significant sequence heterogeneity.
authors.affiliation: Liver Research Center, Rhode Island Hospital, Brown University, Providence, Rhode Island 02903, USA.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Qin
authors.forename: Jiming
authors.initials: J
authors.lastname: Zhang
authors.forename: Tamako
authors.initials: T
authors.lastname: Garcia
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Ito
authors.forename: Danielle
authors.initials: D
authors.lastname: Gutelius
authors.forename: Jisu
authors.initials: J
authors.lastname: Li
authors.forename: Jack
authors.initials: J
authors.lastname: Wands
authors.forename: Shuping
authors.initials: S
authors.lastname: Tong
mesh_terms: D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D016679:Genome, Viral; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D014764:Viral Proteins