title: Kinetic resolution of 2-hydroxybutanoate racemic mixtures by NAD-independent L-lactate dehydrogenase.
abstract: Optically active D-2-hydroxybutanoate is an important building block intermediate for medicines and biodegradable poly(2-hydroxybutanoate). Kinetic resolution of racemic 2-hydroxybutanoate may be a green and desirable alternative for D-2-hydroxybutanoate production. In this work, D-2-hydroxybutanoate at a high concentration (0.197 M) and a high enantiomeric excess (99.1%) was produced by an NAD-independent L-lactate dehydrogenase (L-iLDH) containing biocatalyst. 2-Oxobutanoate, another important intermediate, was co-produced at a high concentration (0.193 M). Using a simple ion exchange process with the macroporous anion exchange resin D301, D-2-hydroxybutanoate was separated from the biotransformation system with a high recovery of 84.7%.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000327:Adsorption; D002087:Butyrates / Q000378:metabolism; D002384:Catalysis; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007475:Ion Exchange Resins; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D011549:Pseudomonas; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D002087:Butyrates; D006885:Hydroxybutyrates; D007475:Ion Exchange Resins; D013778:Tetrazolium Salts; D013844:Thiazoles; C005087:alpha-ketobutyric acid; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; C031570:2-hydroxybutyric acid