title: Does domestication process affect stress response in juvenile Eurasian perch Perca fluviatilis?
abstract: The objective was to evaluate the impact of domestication process on the physiological stress response of cultured Eurasian perch confronted to a chronic stress situation. Briefly, F1 and F4 juveniles were submitted to chronic confinement and investigated on days 5, 15 and 30. Capture and 15min-anesthesia were imposed on fish to assess the effect of preceding confinement on acute stress response. On day 30, the fish were finally challenged with Aeromonas hydrophila and sampled after 5 and 10 days for immune parameter measurements. Cortisol and glucose levels were not affected by confinement but increased significantly after acute stressor exposure. Moreover, cortisol rise following capture and anesthesia was higher in F1 confined-fish, suggesting that they have previously been affected by chronic confinement. A higher HSP70 level was also observed on day 30 in F1 confined-juveniles. During bacterial challenge, regardless of confinement level, F4 juveniles displayed higher lysozyme activity and agglutination response than F1 which may indicate a higher immune capacity in domesticated fish. In conclusion, chronic confinement stressor induced few physiological responses but may increase the responsiveness to other aquacultural stressors. Domestication process also seems to improve chronic stress resistance, growth as well as the immune status of the fish.
authors.affiliation: University of Namur (FUNDP), Unité de Recherches en Biologie des Organismes (URBO), Rue de Bruxelles, 61, B-5000, Namur, Belgium. jessica.douxfils@fundp.ac.be
authors.forename: J
authors.initials: J
authors.lastname: Douxfils
authors.forename: S N M
authors.initials: SN
authors.lastname: Mandiki
authors.forename: G
authors.initials: G
authors.lastname: Marotte
authors.forename: N
authors.initials: N
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Silvestre
authors.forename: S
authors.initials: S
authors.lastname: Milla
authors.forename: E
authors.initials: E
authors.lastname: Henrotte
authors.forename: M
authors.initials: M
authors.lastname: Vandecan
authors.forename: C
authors.initials: C
authors.lastname: Rougeot
authors.forename: C
authors.initials: C
authors.lastname: Mélard
authors.forename: P
authors.initials: P
authors.lastname: Kestemont
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D001786:Blood Glucose / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006854:Hydrocortisone / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009113:Muramidase / Q000097:blood; D010471:Perches / Q000378:metabolism / Q000382:microbiology / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D007334:Insulin-Like Growth Factor I; D009113:Muramidase; D006854:Hydrocortisone