title: Development of oleic acid-functionalized magnetite nanoparticles as hydrophobic probes for concentrating peptides with MALDI-TOF-MS analysis.
abstract: The oleic acid-functionalized magnetite nanoparticles (OA-Fe(3) O(4) ) with mean diameter of about 15 nm were synthesized through a low-cost, one-pot method and were designed as hydrophobic probes to realize the convenient, efficient and fast concentration of low-concentration peptides followed by MALDI-TOF-MS analysis. The capability of OA-Fe(3) O(4) nanoparticles in concentration of low-abundance peptides from simple and complex solutions were evaluated by comparing them with a sort of C8-modified magnetic microspheres. Samples of standard peptide solution, protein digest solution and human serum were introduced in the evaluating process, and the OA-Fe(3) O(4) nanoparticles exhibited good surface affinity toward low-concentration peptides.
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai, P. R. China.
authors.forename: Hemei
authors.initials: H
authors.lastname: Chen
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D019301:Oleic Acid / Q000737:chemistry; D010316:Particle Size; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012015:Reference Standards; D044967:Serum / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010455:Peptides; D011506:Proteins; C000499:ferric oxide; D019301:Oleic Acid