title: Responses of the bacterial community to chronic gamma radiation in a rice paddy ecosystem.
abstract: OBJECTIVE
  Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated.
  
  
  METHODS
  Paddy field microcosm samples were continuously exposed at a dose rate of 1 Gy day(-1) for five days after which the bacterial community structures in them were compared with those of unirradiated control samples by denaturing gradient gel electrophoresis (DGGE) of the 16S ribosomal ribonucleic acid (rRNA) gene. Dissolved ions in ponding waters of the microcosm samples were determined by ion chromatography.
  
  
  RESULTS
  The DGGE band profiles were clearly different between the control and exposure samples. Sequencing of selected DGGE bands revealed that a specific species of the genus Clostridium and of the genus Massilia were grown under radiation exposure. Additionally, the concentrations of sulphate ion in the ponding water of the microcosm samples increased under radiation exposure. Concentrations of Na(+), Ca(2+), and F(-) inversely decreased.
  
  
  CONCLUSIONS
  The data presented suggest that chronic gamma radiation affects the bacterial community structure and material cycles in the rice paddy ecosystem.
authors.affiliation: Environmental Radiation Effects Research Group, National Institute of Radiological Sciences, Chiba, Japan. nobu@nirs.go.jp
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Ishii
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fuma
authors.forename: Keiko
authors.initials: K
authors.lastname: Tagami
authors.forename: Shino
authors.initials: S
authors.lastname: Honma-Takeda
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shikano
mesh_terms: D000383:Agriculture; D018407:Bacterial Physiological Phenomena / Q000528:radiation effects*; D044822:Biodiversity; D017753:Ecosystem; D005720:Gamma Rays; D012275:Oryza / Q000382:microbiology* / Q000528:radiation effects*; D012329:RNA, Bacterial / Q000235:genetics* / Q000528:radiation effects*; D011829:Radiation Dosage
chemical_list: D012329:RNA, Bacterial