title: Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species.
abstract: Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, 7 Southern East Lake Road, Wuchang, Wuhan, Hubei 430072, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Rao
authors.forename: Liqiao
authors.initials: L
authors.lastname: Zhong
authors.forename: Tao
authors.initials: T
authors.lastname: Liao
authors.forename: Shiwei
authors.initials: S
authors.lastname: Jin
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.forename: Sean M
authors.initials: SM
authors.lastname: Hemmingsen
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Heping
authors.initials: H
authors.lastname: Dai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003530:Cyprinidae / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014819:Vitellogenins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D014819:Vitellogenins