title: Imbalanced dietary ascorbic acid alters molecular pathways involved in skeletogenesis of developing European sea bass (Dicentrarchus labrax).
abstract: The influence of dietary ascorbic acid (AA) on growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45days post hatching. Diets incorporated 0, 5, 15, 30, 50 or 400mg AA per kg diet to give AA-0, AA-5, AA-15, AA-30, AA-50 and AA-400 dietary treatments, respectively. Dietary AA levels lower than 15mg/kg reduced larval growth and survival was affected in specimens fed diets devoid of AA. Globally, disruption of the expression of genes involved in AA and calcium absorption in the intestine (SVCT-1, TRPV-6), skeletogenesis (BMP-4, IGF-1, RARγ) and bone mineralization (VDRβ, osteocalcin) were observed in groups fed doses lower and higher than 50mg AA/kg diet. Such disturbances detected at molecular level were associated with disruptions of the ossification process and the appearance of skeletal abnormalities.
authors.affiliation: Ifremer Marine Fish Nutrition Team, Nutrition Aquaculture and Genomics Research Unit, UMR 1067, Ifremer, Technopole Brest-Iroise, BP 70, 29280 Plouzané, France. maria.darias@irta.cat
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Darias
authors.forename: David
authors.initials: D
authors.lastname: Mazurais
authors.forename: Giorgos
authors.initials: G
authors.lastname: Koumoundouros
authors.forename: Marie M
authors.initials: MM
authors.lastname: Le Gall
authors.forename: Christine
authors.initials: C
authors.lastname: Huelvan
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Desbruyeres
authors.forename: Patrick
authors.initials: P
authors.lastname: Quazuguel
authors.forename: Chantal L
authors.initials: CL
authors.lastname: Cahu
authors.forename: Jose L
authors.initials: JL
authors.lastname: Zambonino-Infante
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001492:Bass / Q000235:genetics* / Q000254:growth & development; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics; D004032:Diet; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D007814:Larva / Q000235:genetics / Q000254:growth & development; D029363:Organic Anion Transporters, Sodium-Dependent / Q000235:genetics; D015675:Osteocalcin / Q000235:genetics; D010012:Osteogenesis / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D050916:TRPV Cation Channels / Q000235:genetics; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology; D000097768:Retinoic Acid Receptor gamma
chemical_list: D001205:Ascorbic Acid; D055415:Bone Morphogenetic Protein 4; D029941:Fish Proteins; D007334:Insulin-Like Growth Factor I; D029363:Organic Anion Transporters, Sodium-Dependent; D015675:Osteocalcin; D018167:Receptors, Calcitriol; D018168:Receptors, Retinoic Acid; D050916:TRPV Cation Channels; D014815:Vitamins; D000097768:Retinoic Acid Receptor gamma; C120776:TRPV6 channel