title: Neutrophil depletion reduces blood-brain barrier breakdown, axon injury, and inflammation after intracerebral hemorrhage.
abstract: Neutrophils are thought to contribute to damage after intracerebral hemorrhage (ICH), but there is little direct evidence for this. We depleted circulating blood neutrophils with an anti-polymorphonuclear leukocyte antibody (anti-PMN) before inducing ICH in the rat striatum. Neutrophil infiltration, which was mainly at the edge of the hematoma, was decreased by more than 60% by anti-PMN mediated depletion. We then analyzed neutrophil contributions to BBB breakdown, white matter damage (axons and myelin), and glial and inflammatory responses, both spatially and temporally. Neutrophil depletion reduced BBB leakiness in the peri-hematoma region. Matrix metalloprotease 9, which is thought to contribute to BBB breakdown, was restricted to neutrophils after ICH and was thus reduced by neutrophil depletion. Early perihematomal axonal injury seen at 1 and 3 days after ICH was decreased by depleting neutrophils, and at later times (7 and 14 days), the astrocytic and microglia/macrophage responses were reduced in the perihematoma region and the surrounding striatum. Detailed spatial analysis showed that neutrophil depletion reduced infiltration of activated microglia/macrophages in the peri-hematoma white matter tracts and decreased myelin fragmentation and axon damage. These results show that, in experimental ICH, neutrophils produce matrix metalloprotease9and contribute to blood vessel disruption, BBB breakdown, axon damage, and astrocytic and microglial/macrophage responses that evolve after ICH.
authors.affiliation: Toronto Western Research Institute, University Health Network, Toronto, Canada.
authors.forename: Iska
authors.initials: I
authors.lastname: Moxon-Emre
authors.forename: Lyanne C
authors.initials: LC
authors.lastname: Schlichter
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology*; D001812:Blood-Brain Barrier / Q000276:immunology / Q000473:pathology*; D002135:Calcium-Binding Proteins / Q000276:immunology; D002543:Cerebral Hemorrhage / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007249:Inflammation / Q000276:immunology / Q000473:pathology*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008840:Microfilament Proteins; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D007074:Immunoglobulin G; D008840:Microfilament Proteins; D020780:Matrix Metalloproteinase 9