title: Effects of resveratrol on ileal smooth muscle reactivity in polymicrobial sepsis model.
abstract: OBJECTIVE
  To determine the effects of resveratrol on the ileal smooth muscle reactivity in polymicrobial sepsis.
  
  
  METHODS
  Polimicrobial sepsis was induced by the cecal ligation and perforation (CLP) procedure. Sprague Dawley rats were divided into three groups. Rats in resveratrol group received resveratrol after CLP (100 mg/kg, i.p.). Rats received saline immediately after CLP in the sepsis group. Control group rats underwent sham operation. The rats were sacrificed and the ileum was excised 24 h after the operation. Contractile and relaxant responses in isolated smooth muscle strips (SMS) were determined using an in vitro muscle technique. TNFα and IL-6 levels were measured in blood samples.
  
  
  RESULTS
  Contractile responses to carbachol and KCl and relaxant responses to transmural electrical field stimulation (EFS) were significantly decreased in the sepsis group compared with control and resveratrol groups. No significant changes were observed for smooth muscle reactivity in the resveratrol and control groups. Sodium nitroprusside (SNP) or papaverine-induced relaxations were similar in the all groups. Resveratrol treatment supressed increased TNFα and IL-6 levels in blood seen in sepsis group.
  
  
  CONCLUSIONS
  Ileal smooth muscle reactivity was improved after resveratrol treatment in rats with sepsis. The results of the present study indicate that the beneficial effects of resveratrol might be, at least in part, attributed to its effects on non-adrenergic non-cholinergic pathway and/or anti-inflammatory and antioxidant activity.
authors.affiliation: Department of Pharmacology, Kocaeli University Medical Faculty, Kocaeli, Turkey.
authors.forename: Nejat
authors.initials: N
authors.lastname: Gacar
authors.forename: Selcen
authors.initials: S
authors.lastname: Gocmez
authors.forename: Tijen
authors.initials: T
authors.lastname: Utkan
authors.forename: Gulcin
authors.initials: G
authors.lastname: Gacar
authors.forename: Ipek
authors.initials: I
authors.lastname: Komsuoglu
authors.forename: Melih
authors.initials: M
authors.lastname: Tugay
authors.forename: N Zafer
authors.initials: NZ
authors.lastname: Utkan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002432:Cecum / Q000601:surgery; D007082:Ileum / Q000187:drug effects*; D007375:Interleukin-1 / Q000097:blood; D008026:Ligation; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D018805:Sepsis / Q000188:drug therapy*; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D007375:Interleukin-1; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D000077185:Resveratrol