title: Sperm chromatin condensation in infertile men with varicocele before and after surgical repair.
abstract: OBJECTIVE
  To assess sperm chromatin integrity in infertile men with varicocele before and after surgical repair.
  
  
  METHODS
  Prospective.
  
  
  METHODS
  Academic setting.
  
  
  METHODS
  Seventy-two infertile men with varicocele compared with 20 healthy fertile men.
  
  
  METHODS
  History taking, genital examination, semen analysis, sperm chromatin condensation assessment by aniline blue stain before and 3 months after varicocelectomy.
  
  
  METHODS
  Stained sperm heads (abnormal chromatin condensation) before and 3 months after varicocelectomy.
  
  
  RESULTS
  The mean percentage of aniline blue-stained sperm heads was significantly higher in infertile men with varicocele compared with fertile controls. The mean percentage of stained sperm heads was significantly decreased in infertile men with varicocele 3 months after surgery compared with the preoperative data. There was a significant negative correlation between percentage of stained sperm heads and normal morphology where nonsignificant correlation was elicited regarding sperm count and sperm motility.
  
  
  CONCLUSIONS
  There is a significant increase of abnormal sperm chromatin condensation in infertile men with varicocele that is markedly improved after varicocelectomy.
authors.affiliation: Department of Dermatology and Andrology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Sadek
authors.forename: Abdel Shakour A
authors.initials: AS
authors.lastname: Almohamdy
authors.forename: Amr
authors.initials: A
authors.lastname: Zaki
authors.forename: Mohamed
authors.initials: M
authors.lastname: Aref
authors.forename: Shady M
authors.initials: SM
authors.lastname: Ibrahim
authors.forename: Taymour
authors.initials: T
authors.lastname: Mostafa
mesh_terms: D000328:Adult; D002843:Chromatin / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D042003:DNA Packaging / Q000502:physiology; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000235:genetics* / Q000378:metabolism / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D055101:Semen Analysis / Q000379:methods; D013091:Spermatogenesis / Q000235:genetics; D013094:Spermatozoa / Q000378:metabolism*; D013521:Urologic Surgical Procedures, Male; D014646:Varicocele / Q000150:complications / Q000235:genetics* / Q000378:metabolism / Q000601:surgery*; D055815:Young Adult
chemical_list: D002843:Chromatin