title: Novel nested direct PCR technique for malaria diagnosis using filter paper samples.
abstract: The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.
authors.affiliation: Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Fuehrer
authors.forename: Markus A
authors.initials: MA
authors.lastname: Fally
authors.forename: Verena E
authors.initials: VE
authors.lastname: Habler
authors.forename: Peter
authors.initials: P
authors.lastname: Starzengruber
authors.forename: Paul
authors.initials: P
authors.lastname: Swoboda
authors.forename: Harald
authors.initials: H
authors.lastname: Noedl
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001769:Blood / Q000469:parasitology*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003362:Cost-Benefit Analysis; D003890:Desiccation; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008288:Malaria / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D010209:Paper; D010274:Parasitology / Q000379:methods*; D010961:Plasmodium / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D055815:Young Adult