title: A multipathogen selective enrichment broth for simultaneous growth of Salmonella spp., Vibrio parahaemolyticus, and Vibrio cholerae.
abstract: A selective enrichment broth (SVV) was formulated to allow concurrent growth of Salmonella spp., V. parahaemolyticus, and V. cholerae. Potassium tellurite and sodium citrate were added as the inhibitors, while glucose, mannitol, anhydrous sodium sulfite and sodium pyruvate were employed as the growth-promoters. When mixed in equal or varied proportions, the target pathogens in SVV had a great accumulation (10(5)-10(8) CFU/ml) and effectively inhibited the growth of competitive microflora. In the artificially contaminated samples, a high recovery of these 3 target pathogens was obtained in SVV. Finally, Salmonella spp., V. parahaemolyticus, and V. cholerae were detected from 608 suspicious food samples by SVV with real-time PCR, and no false-positive or -negative results were reported. In summary, SVV has been shown to be a suitable broth for the simultaneous detection of the 3 pathogens by multipathogen detection on a single-assay platform.
authors.affiliation: Institution of Food Safety, College of Light Industry & Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Xiao
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Li
authors.forename: Yi-Ying
authors.initials: YY
authors.lastname: Qin
authors.forename: Yi-Gang
authors.initials: YG
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D002241:Carbohydrates; D002951:Citrates; D003470:Culture Media; D005516:Food Microbiology; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000254:growth & development* / Q000302:isolation & purification; D000077559:Sodium Citrate; D013691:Tellurium; D014734:Vibrio cholerae / Q000254:growth & development* / Q000302:isolation & purification; D014736:Vibrio parahaemolyticus / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D002951:Citrates; D003470:Culture Media; D000077559:Sodium Citrate; C026899:potassium tellurate(IV); D013691:Tellurium