title: Acid sphingomyelinase contributes to evodiamine-induced apoptosis in human gastric cancer SGC-7901 cells.
abstract: Evodiamine-induced apoptosis has been shown to have anticancer activity by eradication of some carcinoma cell lines. This study was designed to evaluate the effects of evodiamine on the viability of human gastric cancer SGC-7901 cells and to define the cell death pathway. Flow cytometry detection showed that 1.5 μM evodiamine significantly induced SGC-7901 cell apoptosis in a time-dependent manner. This apoptosis was partially inhibited by the pancaspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoro-methylketone, which suggests that evodiamine-induced apoptosis in SGC-7901 cells is partially caspase independent. Further, the total content of sphingomyelin was decreased and expression of acid sphingomyelinase (aSMase) and neutral SMase genes in the SGC-7901cells was upregulated. Protein expression of aSMase, which was exposed to evodiamine, was shown to be increased by western blot analysis and could have been responsible for inducing caspase-independent apoptosis. Our results indicate that evodiamine stimulates upregulation of aSMase expression and hydrolysis of sphingomyelin into ceramide, which might be one of the mechanisms by which apoptosis occurs in SGC-7901 cells.
authors.affiliation: Department of Clinical Biochemistry, Guiyang Medical College, No. 9 Beijing Road, Guiyang, China. huanghai828@gmc.edu.cn
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Yunyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Li
authors.forename: Wenfen
authors.initials: W
authors.lastname: Xu
authors.forename: Shunzhi
authors.initials: S
authors.lastname: He
authors.forename: Yunzhu
authors.initials: Y
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D015153:Blotting, Western; D020169:Caspases; D045744:Cell Line, Tumor; D002518:Ceramides / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D011799:Quinazolines / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013108:Sphingomyelin Phosphodiesterase / Q000235:genetics / Q000378:metabolism*; D013109:Sphingomyelins / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; D002518:Ceramides; D010936:Plant Extracts; D011799:Quinazolines; D013109:Sphingomyelins; C049639:evodiamine; D013108:Sphingomyelin Phosphodiesterase; D020169:Caspases