title: Light scattering of human red blood cells during metabolic remodeling of the membrane.
abstract: We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP.
authors.affiliation: Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. yk.park@kaist.ac.kr
authors.forename: YongKeun
authors.initials: Y
authors.lastname: Park
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Best-Popescu
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Gabriel
authors.initials: G
authors.lastname: Popescu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D008027:Light; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012542:Scattering, Radiation
chemical_list: D000255:Adenosine Triphosphate