title: Solution NMR structure and dynamics of human apo-S100A1 protein.
abstract: S100A1 belongs to the EF-hand superfamily of calcium binding proteins. It is a representative of the S100 protein family based on amino acid sequence, three-dimensional structure, and biological function as a calcium signal transmitter. It is a homodimer of noncovalently bound subunits. S100A1, like most of other members of the S100 protein family, is a multifunctional, regulatory protein involved in a large variety of biological processes and closely associated with several human diseases. The three-dimensional structure of human apo-(i.e. calcium free)-S100A1 protein was determined by NMR spectroscopy (PDB 2L0P) and its backbone dynamics established by ¹⁵N magnetic relaxation. Comparison of these results with the structure and backbone dynamics previously determined for bovine apo-S100A1 protein modified by disulfide formation with β-mercaptoethanol at Cys 85 revealed that the secondary structure of both these proteins was almost identical, whereas the global structure of the latter was much more mobile than that of human apo-S100 protein. Differences between the structures of human and rat apo-S100A1 are also discussed.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowakowski
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Jaremko
authors.forename: Mariusz
authors.initials: M
authors.lastname: Jaremko
authors.forename: Igor
authors.initials: I
authors.lastname: Zhukov
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Belczyk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bierzyński
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ejchart
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry*; D002417:Cattle; D006801:Humans; D008623:Mercaptoethanol / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D009418:S100 Proteins / Q000737:chemistry*
chemical_list: D001059:Apoproteins; D009418:S100 Proteins; C110294:S100A1 protein; D008623:Mercaptoethanol