title: Increased matrix metalloproteinases-1,-9 in the uterosacral ligaments and vaginal tissue from women with pelvic organ prolapse.
abstract: OBJECTIVE
  To investigate the possible association of increased matrix metalloproteinases (MMPs)-1,-9 with pelvic organ prolapse (POP) and to evaluate whether inflammatory processes contribute to its development.
  
  
  METHODS
  Forty women who underwent hysterectomy, 20 with POP grade 2 and above, and 20 without POP, participated in the study. Biopsies from the uterosacral ligaments and vaginal mucosa were obtained from each woman. Each biopsy was sectioned and stained for MMP-1 and MMP-9 by immunohistochemical methods and with hematoxylin and eosin (H&E). MMP-1,-9 expressions were evaluated on the immunostained slides. H&E stained sections were examined for possible inflammatory changes.
  
  
  RESULTS
  A higher stromal (extra-cellular) expression of MMPs-1,-9 was found in POP cases compared with controls in vaginal biopsies (MMP-1: p=0.004; MMP-9: p=0.042) as well as in uterosacral ligament biopsies (MMP-1: p=0.011; MMP-9: p=0.015). Increased intracellular expression of both MMPs was also demonstrated in fibroblasts in biopsies of women with POP (p<0.001 for all). Most of these differences persisted after controlling for age. The degree of inflammatory changes reflected by the number of lymphocytes, plasma cells and capillary-sized blood vessels per 10 high power fields, was similar in specimens obtained from women with and without POP.
  
  
  CONCLUSIONS
  The expression of MMPs-1,-9 appears to be increased in tissues from women with POP. This supports an association, although not a causal relation, between increased MMPs-1,-9 and POP. Inflammation does not seem to play an important role in the pathogenesis of POP.
authors.affiliation: Medical School, Faculty of Health Sciences, Ben-Gurion University, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Dviri
authors.forename: Elad
authors.initials: E
authors.lastname: Leron
authors.forename: Jacob
authors.initials: J
authors.lastname: Dreiher
authors.forename: Moshe
authors.initials: M
authors.lastname: Mazor
authors.forename: Ruthy
authors.initials: R
authors.lastname: Shaco-Levy
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008022:Ligaments / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008875:Middle Aged; D009092:Mucous Membrane / Q000201:enzymology / Q000473:pathology; D056887:Pelvic Organ Prolapse / Q000201:enzymology* / Q000276:immunology; D012447:Sacrum; D012720:Severity of Illness Index; D017154:Stromal Cells / Q000201:enzymology / Q000473:pathology; D014599:Uterus; D014621:Vagina / Q000201:enzymology* / Q000276:immunology / Q000473:pathology
chemical_list: D015415:Biomarkers; D020780:Matrix Metalloproteinase 9; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1