title: Resistance of Fasciola hepatica against triclabendazole in cattle in Patagonia (Argentina).
abstract: In the winter of 2008, cattle on a farm in the province of Neuquen, Argentina died from subacute and chronic liver fluke disease despite four previous treatments with Triclabendazole (TCBZ). In the spring of 2009, a preliminary efficacy test revealed good performance using nitroxynil, whereas TCBZ efficacy was only 18% by egg counts of Fasciola eggs in the faeces. Resistance to Fasciola hepatica to TCBZ has never been reported in South America, so in January of 2010 a controlled trial was conducted to confirm and to define the degree of resistance in this herd. In a clinical trial, the fluke egg output was monitored on Days 14 and 21 and serum enzymes gamma-glutamyl transpeptidase (GGT) and glutamic-oxaloacetic transaminase (GOT) on Days 0 and 21 in 36 calves treated with TCBZ or with closantel. The results showed a reduction of 100% in fluke egg output at Days 14 and 21 for closantel. The mean epg in the TCBZ-treated groups did not decrease. Because of the fact that in this study TCBZ treatment in cattle had no effect, even at double the recommended dose, it is highly indicative that resistance of F. hepatica against TCBZ is present on this farm. The GGT and GOT levels decreased in the closantel-treated group as a result of the treatment at 21 days after dosing. To evaluate the importance of TCBZ resistance in F. hepatica in Argentina, a study on more farms from endemic areas is needed.
authors.affiliation: Animal Health Group, INTA Bariloche, Río Negro, CC. 277 (8400) Bariloche, Argentina. folaechea@bariloche.inta.gov.ar
authors.forename: F
authors.initials: F
authors.lastname: Olaechea
authors.forename: V
authors.initials: V
authors.lastname: Lovera
authors.forename: M
authors.initials: M
authors.lastname: Larroza
authors.forename: F
authors.initials: F
authors.lastname: Raffo
authors.forename: R
authors.initials: R
authors.lastname: Cabrera
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology* / Q000627:therapeutic use; D001118:Argentina; D001219:Aspartate Aminotransferases / Q000097:blood; D001562:Benzimidazoles / Q000494:pharmacology* / Q000627:therapeutic use; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000201:enzymology / Q000469:parasitology*; D004351:Drug Resistance; D005210:Fasciola hepatica / Q000254:growth & development*; D005211:Fascioliasis / Q000188:drug therapy / Q000201:enzymology / Q000469:parasitology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D008107:Liver Diseases / Q000188:drug therapy / Q000201:enzymology / Q000469:parasitology / Q000662:veterinary*; D010270:Parasite Egg Count / Q000662:veterinary; D012458:Salicylanilides / Q000494:pharmacology* / Q000627:therapeutic use; D000077682:Triclabendazole; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D012458:Salicylanilides; D000077682:Triclabendazole; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; C023342:closantel