title: Isolation and identification of compounds responsible for antioxidant capacity of Euryale ferox seeds.
abstract: Euryale ferox seed is consumed medicinally or for food in China. The present study revealed it to contain significant antioxidant activity, which may be associated with its medical applications as a proteinuria inhibitor of diabetic nephropathy. This study resulted in the identification of 3 new sesquineolignans, named euryalins A-C (1-3), and 16 known compounds, which were all first isolated from this plant apart from 5,7,4-trihydroxy-flavanone. The antioxidant potential of the partial isolates was evaluated using the DPPH radical scavenging assay and mesangial cellular assay. Compounds 2, rel-(2α,3β)-7-O-methylcedrusin (4), syringylglycerol-8-O-4-(sinapyl alcohol) ether (5), and (+)-syringaresinol (7) were found to be most active on DPPH assay, whereas compounds 2, 4, 7, (1R,2R,5R,6S)-2-(3,4-dimethoxyphenyl)-6-(3,4-dihydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane, and buddlenol E could significantly inhibit high glucose-stimulated reactive oxygen species production in mesangial cells. The results suggested that E. ferox seed could be considered as an excellent source of natural antioxidants and is useful in the prevention of diabetic nephropathy.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650204, People's Republic of China.
authors.forename: Chang-Wei
authors.initials: CW
authors.lastname: Song
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhou
authors.forename: Fan-Fan
authors.initials: FF
authors.lastname: Hou
authors.forename: Kai-Jin
authors.initials: KJ
authors.lastname: Wang
authors.forename: Quan-Bin
authors.initials: QB
authors.lastname: Han
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Yong-Xian
authors.initials: YX
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification*; D001713:Biphenyl Compounds; D002460:Cell Line; D003928:Diabetic Nephropathies / Q000517:prevention & control; D005920:Glomerular Mesangium / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D017705:Lignans / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification*; D030702:Nymphaeaceae / Q000737:chemistry*; D008517:Phytotherapy; D010851:Picrates; D010936:Plant Extracts / Q000008:administration & dosage; D011507:Proteinuria / Q000517:prevention & control; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D017705:Lignans; D010851:Picrates; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C004931:1,1-diphenyl-2-picrylhydrazyl; D005947:Glucose