title: In vivo trapping of polyketide intermediates from an assembly line synthase using malonyl carba(dethia)-N-acetyl cysteamines.
abstract: Early-stage intermediates in the biosynthesis of erythromycin A by Saccharopolyspora erythraea were intercepted by malonyl carba(dethia)-N-acetyl cysteamines, generated in vivo from the hydrolysis of the corresponding methyl esters.
authors.affiliation: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
authors.forename: Manuela
authors.initials: M
authors.lastname: Tosin
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Demydchuk
authors.forename: James S
authors.initials: JS
authors.lastname: Parascandolo
authors.forename: Covadonga Blasco
authors.initials: CB
authors.lastname: Per
authors.forename: Finian J
authors.initials: FJ
authors.lastname: Leeper
authors.forename: Peter F
authors.initials: PF
authors.lastname: Leadlay
mesh_terms: D055162:Biocatalysis; D003543:Cysteamine / Q000737:chemistry* / Q000378:metabolism*; D004917:Erythromycin / Q000096:biosynthesis*; D048630:Polyketide Synthases / Q000378:metabolism*; D016930:Saccharopolyspora / Q000201:enzymology
chemical_list: D003543:Cysteamine; D004917:Erythromycin; D048630:Polyketide Synthases