title: Light- and sodium azide-induced death of RGC-5 cells in culture occurs via different mechanisms.
abstract: Previous studies have shown that light impinging on the retina in situ has the capacity to kill neuronal and non-neuronal cells in vitro by interacting directly with mitochondrial constituents. A number of fluorophores are associated with mitochondria which can potentially absorb different wave-lengths of light, including cytochrome oxidase. The aim of the present study was to compare the death mechanism of a light insult to RGC-5 cells in culture with that of sodium azide. Sodium azide's main toxic action is in inhibiting the function of cytochrome oxidase in the mitochondrial electron transport chain. Our studies showed that light and sodium azide kill RGC-5 cells via different mechanisms although some similarities do occur. Both inducers of cell death caused the generation of reactive oxygen species (ROS), the expression of phosphatidylserine, the breakdown of DNA and the activation of p38 MAPK, resulting in its translocation from the nucleus to the cytoplasm. However, light-induced cell death occurs via necroptosis, in that it was inhibited by necrostatin-1 and was caspase-independent. This was not the case for sodium azide, where the death process was caspase-dependent, occurred via apoptosis and was unaffected by necrostatin-1. Moreover, light caused an activation of the apoptosis inducing factor (AIF), c-Jun, JNK and HO-1, but it did not affect alpha fodrin or caspase-3. In contrast, sodium azide caused the activation of alpha fodrin and the stimulation of caspase-3 content without influencing AIF, c-Jun, JNK or HO-1. Therefore we conclude that light does not have a specific action on cytochrome oxidase in mitochondria to cause cell death.
authors.affiliation: Nuffield Laboratory of Ophthalmology, John Radcliffe Hospital, University of Oxford, Level 6, West Wing, Headley Way, Oxford, UK.
authors.forename: Dan
authors.initials: D
authors.lastname: Ji
authors.forename: Tengku A
authors.initials: TA
authors.lastname: Kamalden
authors.forename: Susana
authors.initials: S
authors.lastname: del Olmo-Aguado
authors.forename: Neville N
authors.initials: NN
authors.lastname: Osborne
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology; D008027:Light; D010718:Phosphatidylserines / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000187:drug effects* / Q000528:radiation effects; D019810:Sodium Azide / Q000494:pharmacology*; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D007093:Imidazoles; D007211:Indoles; D010718:Phosphatidylserines; D017382:Reactive Oxygen Species; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; C507699:necrostatin-1; C007293:DAPI; D002392:Catechin; D004247:DNA; D019810:Sodium Azide; C045651:epigallocatechin gallate; D053148:Caspase 3; D019311:Staurosporine