title: [Preparation of a decellularized rat liver scaffold and its biocompatibility].
abstract: OBJECTIVE
  To develop a novel method for preparing decellularized liver biological scaffold (DLBS) for liver tissue engineering.
  
  
  METHODS
  DLBS was prepared by treatment of rat livers with detergent and enzymatic cell extraction and observed under optical and scanning electron microscopes. To assess the biocompatibility of the product, C3A cells and bone marrow-derived mesenchymal cells (BM-MSCs) were cocultured with DLBS as the scaffold, and the effect of DLBS on the proliferation of C3A cells was evaluated by MTT assay. DLBS was also implanted under the dorsal skin of SD rats to evaluate the tissue biocompatibility of this material.
  
  
  RESULTS
  Application of the detergent and enzymatic extraction allowed full extraction of the cells in the liver, leaving an extracellular matrix scaffold composed mainly of collagen and elastic fibrin. The coculture experiment showed that C3A cells and BM-MSCs could grow on and adhere to DLBS. The result of MTT assay showed that DLBS could promote the proliferation of C3A cells.
  
  
  CONCLUSIONS
  This cell-free DLBS, which retains intact extracellular matrix and promotes cell attachment, proliferation, growth and differentiation, can be an ideal biological matrix scaffold material.
authors.affiliation: Second Department of Hepatobiliary Surgery, Southern Medical University, Guangzhou 510282, China. pmxwxy@sohu.com
authors.forename: Ming-xin
authors.initials: MX
authors.lastname: Pan
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Guo-lin
authors.initials: GL
authors.lastname: He
authors.forename: Peng-yun
authors.initials: PY
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002478:Cells, Cultured; D018920:Coculture Techniques; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D008099:Liver / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials