title: Bfr1p is responsible for tributyltin resistance in Schizosaccharomyces pombe.
abstract: ATP-binding cassette (ABC) transporter plays an important role for resistance against xenobiotics. There are eleven ABC transporter genes in the genome of fission yeast Schizosaccharomyces pombe. We examined the role of ABC transporter against the toxicity of tributyltin chloride (TBT), a widespread environmental pollutant, in cell growth. Among individual ABC transporter mutants, the growth of a mutant deficient in Bfr1p, a plasma membrane-embedded transporter, was extremely sensitive to TBT. The lethal TBT concentration inducing 50% of cell death (LC(50)) was 25 µM for the parent strain and 10.2 µM for the bfr1∆ mutant. Thus, Bfr1p was responsible for TBT resistance in S. pombe.
authors.forename: Koichi
authors.initials: K
authors.lastname: Akiyama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Iwaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Soracom
authors.initials: S
authors.lastname: Chardwiriyapreecha
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano
authors.forename: Sogo
authors.initials: S
authors.lastname: Nishimoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Sugahara
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D004785:Environmental Pollutants / Q000633:toxicity*; D012568:Schizosaccharomyces / Q000187:drug effects*; D029702:Schizosaccharomyces pombe Proteins / Q000502:physiology*; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D018528:ATP-Binding Cassette Transporters; C092496:BFR1 protein, S pombe; D004785:Environmental Pollutants; D029702:Schizosaccharomyces pombe Proteins; D014220:Trialkyltin Compounds; C011559:tributyltin