title: Effect of dentin location and long-term water storage on bonding effectiveness of dentin adhesives.
abstract: Dentin is a variable substrate with properties that change considerable in a single surface. The purpose of this study was to evaluate the bonding effectiveness to these different dentin locations and evaluate these differences over time. After bonding procedures with five different adhesives, small micro-tensile bond strength (µTBS) beams were prepared and dichotomously divided in 'center' and 'periphery' dentin specimens. After 1 week, 3, 6 and 12 months of water storage the µTBS of specimens of each group was determined, enabling a paired study design. The bond strengths of both etch&rinse adhesives were insensitive to regional variability. For the two-step self-etch adhesives, a marked increase in bond strengths was observed with increasing amount of intertubular dentin. Regional variability did not affect the long-term bonding effectiveness for any of the adhesives tested. In conclusion, only for the mild self-etch adhesives, µTBS to 'periphery' dentin was higher than for the 'center' specimens.
authors.affiliation: Leuven BIOMAT Research Cluster, Department of Conservative Dentistry, School of Dentistry, Oral Pathology and Maxillo-facial Surgery, Catholic University of Leuven, Belgium. jan.demunck@med.kuleuven.be
authors.forename: Jan
authors.initials: J
authors.lastname: De Munck
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mine
authors.forename: Marcio
authors.initials: M
authors.lastname: Vivan Cardoso
authors.forename: Aline
authors.initials: A
authors.lastname: De Almeida Neves
authors.forename: Kirsten L
authors.initials: KL
authors.lastname: Van Landuyt
authors.forename: André
authors.initials: A
authors.lastname: Poitevin
authors.forename: Bart
authors.initials: B
authors.lastname: Van Meerbeek
mesh_terms: D000293:Adolescent; D000704:Analysis of Variance; D001840:Dental Bonding / Q000379:methods*; D045662:Dental Etching / Q000379:methods; D003799:Dental Stress Analysis; D003804:Dentin / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008137:Longitudinal Studies; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D019279:Resin Cements / Q000737:chemistry / Q000494:pharmacology*; D013718:Tensile Strength; D014070:Tooth / Q000033:anatomy & histology; D014867:Water / Q000737:chemistry; D055815:Young Adult
chemical_list: C503075:Clearfil Protect Bond; C438313:Clearfil SE Bond; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; C469607:OptiBond FL; D019279:Resin Cements; D014867:Water; C041330:Scotchbond