title: The role of DNA binding with the Cry8Ea1 toxin of Bacillus thuringiensis.
abstract: The Cry8Ea1 protoxin is a DNA-protein complex. Both forms of the Cry8Ea1 toxin (with or without DNA binding) were obtained separately, and their stability and ability to insert into a phospholipid monolayer in vitro were compared. The presence of DNA can prevent the toxin from aggregation. Data regarding the penetration of the Cry8Ea1 toxin and Cry8Ea1 toxin-DNA complex into the air/water interface without a phospholipid monolayer show that the Cry8Ea1 toxin-DNA complex is more likely to move towards the air/water interface and is more hydrophobic. Experiments examining the protein interaction with the phospholipid monolayer show that the ability of the Cry8Ea1 toxin-DNA complex to insert into the lipid bilayer is much greater than that of the Cry8Ea1 toxin without DNA.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, China.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Fuping
authors.initials: F
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001413:Bacillus thuringiensis / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D004731:Endotoxins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D004269:DNA, Bacterial; D004731:Endotoxins