title: Meat-type chickens have a higher efficiency of mitochondrial oxidative phosphorylation than laying-type chickens.
abstract: Meat-type chickens show high feed efficiency and have a very rapid growth rate compared with laying-type chickens. To clarify whether the type-specific difference in feed conversion efficiency is involved in mitochondrial bioenergetics, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of both type chickens. Mitochondria from skeletal muscle of meat-type chickens showed greater substrate oxidation and phosphorylating activities, and less proton leak than those of the laying-type, resulting in a higher efficiency of oxidative phosphorylation. Gene expression and protein content of uncoupling protein (avUCP) but not adenine nucleotide translocase (avANT) gene expression were lower in skeletal muscle mitochondria of meat-type chickens than the laying-type. The current results regarding a higher efficiency of oxidative phosphorylation and UCP content may partially support the high feed efficiency of meat-type chickens.
authors.affiliation: Science of Biological Function, Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. toyomizu@bios.tohoku.ac.jp
authors.forename: Masaaki
authors.initials: M
authors.lastname: Toyomizu
authors.forename: Motoi
authors.initials: M
authors.lastname: Kikusato
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawabata
authors.forename: Md Abul Kalam
authors.initials: MA
authors.lastname: Azad
authors.forename: Eriko
authors.initials: E
authors.lastname: Inui
authors.forename: Taku
authors.initials: T
authors.lastname: Amo
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D001835:Body Weight; D002645:Chickens / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005247:Feeding Behavior; D015870:Gene Expression; D015151:Immunoblotting; D007700:Kinetics; D008297:Male; D008460:Meat; D053078:Membrane Potential, Mitochondrial; D008931:Mitochondria, Muscle / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D000071245:Mitochondrial Uncoupling Proteins; D018482:Muscle, Skeletal / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D030161:Avian Proteins; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; C422907:mitochondrial uncoupling protein, chicken; D000226:Mitochondrial ADP, ATP Translocases