title: Effect of zymosan on antioxidant and immune function of S180 tumor-bearing mice.
abstract: The objective is to study the effect of zymosan on antioxidant and immune function of S(180) tumor-bearing mice. Seventy Kunming mice were randomly divided into seven groups: a normal control group (NC), a tumor control group (TC), three dose groups of zymosan (low, medium, high), a cyclophosphamide (Cy) group, and a combination of zymosan and Cy group. The S(180) tumor-bearing mice model was established by the inoculation of cancer cell suspension subcutaneously in the mouse's right anterior limb. At the 19th day, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activity in liver homogenate were analyzed. The reverse transcriptase-polymerase chain reaction was used to determine the mRNA expression levels of IL-2, TNF-α, and TGF-β(1). The activity of GSH-Px and SOD in the liver increased with the dose of zymosan, whereas the activity of MDA significantly decreased in the higher-dose groups of zymosan, compared to the TC group (P < 0.01). In the zymosan groups, mRNA expression levels in tissues of S(180) tumor-bearing mice were significantly higher for TNF-α and IL-2, but lower for TGFβ(1) than in the Cy or TC group (P < 0.01). The high-dose of zymosan markedly showed a depressant effect on S(180) tumor, enhanced by the action of Cy that increased mRNA expression levels of TNF-α and IL-2. The mechanism of zymosan on the inhibition of tumor growth may be due to its ability to enhance the antioxidant and immune function in a dose-dependent manner.
authors.affiliation: Department of Clinical Nutrition, Changhai Hospital, The First Affiliated Hospital of The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Lin
authors.forename: Deng
authors.initials: D
authors.lastname: Zan
authors.forename: Jing-Jue
authors.initials: JJ
authors.lastname: Yuan
authors.forename: Dong-Lian
authors.initials: DL
authors.lastname: Cai
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D003520:Cyclophosphamide / Q000627:therapeutic use; D005979:Glutathione Peroxidase / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000276:immunology; D013482:Superoxide Dismutase / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015054:Zymosan / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D003520:Cyclophosphamide; D015054:Zymosan; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase