title: Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling.
abstract: We present evidence of a link between interferonβ-1b (IFN-β) and G-protein signaling by demonstrating that IFN-β can induce the expression of the negative regulator of G-protein signaling 1 (RGS1). RGS1 reduces G-protein activation and immune cell migration by interacting with heterotrimeric G-proteins and enhancing their intrinsic GTPase activity. In this study, IFN-β treatment resulted in the induction of RGS1 in peripheral blood mononuclear cells (PBMCs), monocytes, T cells, and B cells. Induction of RGS1 by IFN-β was concentration dependent and observed at both the RNA and protein level. Other members of the RGS family were not induced by IFN-β, and induction of RGS1 required the activation of the IFN receptor. In addition, RGS1 induction was observed in PBMCs obtained from IFN-β-treated multiple sclerosis patients suggesting a possible, as yet unexplored, involvement of G-protein regulation in disease treatment. The upregulation of RGS1 by IFN-β has not been previously reported.
authors.affiliation: Bayer HealthCare, Applied Research, Richmond, CA 94804, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Tran
authors.forename: Pedro
authors.initials: P
authors.lastname: Paz
authors.forename: Sharlene
authors.initials: S
authors.lastname: Velichko
authors.forename: Jill
authors.initials: J
authors.lastname: Cifrese
authors.forename: Praveen
authors.initials: P
authors.lastname: Belur
authors.forename: Ken D
authors.initials: KD
authors.lastname: Yamaguchi
authors.forename: Karin
authors.initials: K
authors.lastname: Ku
authors.forename: Parham
authors.initials: P
authors.lastname: Mirshahpanah
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Reder
authors.forename: Ed
authors.initials: E
authors.lastname: Croze