title: PGD for a complex chromosomal rearrangement by array comparative genomic hybridization.
abstract: Patients carrying a chromosomal rearrangement (CR) have an increased risk for chromosomally unbalanced conceptions. Preimplantation genetic diagnosis (PGD) may avoid the transfer of embryos carrying unbalanced rearrangements, therefore increasing the chance of pregnancy. Only 7-12 loci can be screened by fluorescence in situ hybridization whereas microarray technology can detect genome-wide imbalances at the single cell level. We performed PGD for a CR carrier with karyotype 46,XY,ins(3;2)(p23;q23q14.2),t(6;14)(p12.2;q13) using array comparative genomic hybridization. Selection of embryos for transfer was only based on copy number status of the chromosomes involved in both rearrangements. In two ICSI-PGD cycles, nine and seven embryos were analysed by array, leaving three and one embryo(s) suitable for transfer, respectively. The sensitivity and specificity of single cell arrays was 100 and 88.8%, respectively. In both cycles a single embryo was transferred, resulting in pregnancy following the second cycle. The embryo giving rise to the pregnancy was normal/balanced for the insertion and translocation but carried a trisomy 8 and nullisomy 9 in one of the two biopsied blastomeres. After 7 weeks of pregnancy the couple miscarried. Genetic analysis following hystero-embryoscopy showed a diploid (90%)/tetraploid (10%) mosaic chorion, while the gestational sac was empty. No chromosome 8 aneuploidy was detected in the chorion, while 8% of the cells carried a monosomy for chromosome 9. In summary, we demonstrate the feasibility and determine the accuracy of single cell array technology to test against transmission of the unbalanced meiotic products that can derive from CRs. Our findings also demonstrate that the genomic constitution of extra-embryonic tissue cannot necessarily be predicted from the copy number status of a single blastomere.
authors.affiliation: Center for Human Genetics, UZ Gasthuisberg, 3000 Leuven, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Vanneste
authors.forename: C
authors.initials: C
authors.lastname: Melotte
authors.forename: T
authors.initials: T
authors.lastname: Voet
authors.forename: C
authors.initials: C
authors.lastname: Robberecht
authors.forename: S
authors.initials: S
authors.lastname: Debrock
authors.forename: A
authors.initials: A
authors.lastname: Pexsters
authors.forename: C
authors.initials: C
authors.lastname: Staessen
authors.forename: C
authors.initials: C
authors.lastname: Tomassetti
authors.forename: E
authors.initials: E
authors.lastname: Legius
authors.forename: T
authors.initials: T
authors.lastname: D'Hooghe
authors.forename: J R
authors.initials: JR
authors.lastname: Vermeesch
mesh_terms: D000022:Abortion, Spontaneous / Q000235:genetics; D000328:Adult; D000782:Aneuploidy; D002869:Chromosome Aberrations; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D055028:Comparative Genomic Hybridization / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*