title: Sulfated modification and antioxidant activity of exopolysaccahrides produced by Enterobacter cloacae Z0206.
abstract: Nine modification conditions were designed to sulfate exopolysaccharides (EPS) produced by Enterobacter cloacae Z0206 by chlorosulfonic acid-pyridine (CSA-Pyr) method according to the orthogonal test and focusing on three affecting factors such as the ratio of CSA to Pyr, reaction temperature and reaction time. And nine sulfated derivatives with various degrees of substitution (DS) were obtained. Their antioxidant activities were evaluated in vitro, by scavenging abilities on superoxide radical and hydroxyl radical. The results indicated that sulfated derivatives of EPS showed noticeable effects on scavenging superoxide radical and hydroxyl radical compared with native one, and sulfated derivative with moderate DS of 0.60 showed highest antioxidant activities. The optimum sulfated conditions of EPS were the ratio of CSA to Pyr of 1:2, the reaction time of 2h and reaction temperature of 70 °C.
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, NO. 164 North of Qiutao Road, Hangzhou 310029, PR China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Jin
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Lu
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
authors.forename: Youming
authors.initials: Y
authors.lastname: Wang
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000138:chemical synthesis* / Q000378:metabolism*; D016972:Enterobacter cloacae / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*; D013451:Sulfonic Acids; D013481:Superoxides / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D000975:Antioxidants; D011135:Polysaccharides, Bacterial; D013440:Sulfides; D013451:Sulfonic Acids; D013481:Superoxides; C013880:chlorosulfonic acid; D017665:Hydroxyl Radical