title: Reversed-phase liquid chromatographic separation of antiretroviral drugs on a monolithic column using ionic liquids as mobile phase additives.
abstract: The use of 3-methylimidazolium cation-based ionic liquids (ILs) was evaluated as mobile phase additives for separation of antiretroviral drugs on a monolithic column by RP-HPLC. Separation of eight commonly used antiretroviral drugs was achieved on a Chromolith Flash, RP-18e column (25 × 4.6 mm, porous material) using water (pH 4.0 adjusted with acetic acid)/methanol v/v as a mobile phase containing ILs in a gradient elution mode. The effects of concentrations of ILs on retention, resolution and peak shape were studied and a regression equation correlating the interactions between stationary phase and the ILs was established. The retention of all the drugs was decreased notably by using 1-butyl-3-methylimidazolium tetrafluoroborate, while 1-ethyl-3-methylimidazolium methylsulfate reduced gradient drift drastically when compared to triethylamine.
authors.affiliation: HPLC/UV Group, Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, India. rnrao@iict.res.in
authors.forename: Ramisetti
authors.initials: R
authors.lastname: Nageswara Rao
authors.forename: Bondigalla
authors.initials: B
authors.lastname: Ramachandra
authors.forename: Rajavarapu
authors.initials: R
authors.lastname: Mastan Vali
mesh_terms: D000327:Adsorption; D000998:Antiviral Agents / Q000032:analysis / Q000302:isolation & purification*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D052578:Ionic Liquids