title: Application of ultra-performance columns in high-performance liquid chromatography for determination of albendazole and its metabolites in turkeys.
abstract: Methods for determination of albendazole (ALB), albendazole sulfoxide (SOX) and albendazole sulfone (SON) in turkey blood plasma, using high-performance liquid chromatography (HPLC) with fluorescence detection, were developed. Moreover, comparison of HPLC columns with ultra-performance liquid chromatography (UPLC) columns was performed. Albendazol was administered orally in 5-week-old birds (n = 18) at a dose of 25 mg/kg b.w. Accuracy and precision of the developed method were satisfactory and stability studies showed acceptable variation (below 15%) in ALB, SOX and SON concentrations when the samples were stored at -75°C for 15 days. UPLC(®) columns gave higher peaks from typical HPLC columns retaining high quality of analysis. Pharmacokinetic analysis indicated quick elimination of ALB from turkey blood plasma. The mean residence time of SON was at least two times longer than that of SOX and four times longer than that of ALB. The elimination half-lives for ALB, SOX and SON were 0.7 ± 0.27, 5.37 ± 6.03, 9.17 ± 5.12 h, respectively. The obtained results indicate that the described method allows for precise determination of albendazole and its metabolites in turkey plasma. Moreover, using UPLC columns in HPLC apparatus results in higher sensitivity as compared with the classical HPLC columns.
authors.affiliation: Centre of Pharmacokinetics Research FILAB, Łajski, Poland. t.grabowski@filab.com.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Grabowski
authors.forename: Jerzy Jan
authors.initials: JJ
authors.lastname: Jaroszewski
authors.forename: Anna
authors.initials: A
authors.lastname: Swierczewska
authors.forename: Renata
authors.initials: R
authors.lastname: Sawicka
authors.forename: Tomasz
authors.initials: T
authors.lastname: Maślanka
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Markiewicz
authors.forename: Hubert
authors.initials: H
authors.lastname: Ziółkowski
mesh_terms: D015766:Albendazole / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D000871:Anthelmintics / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D016018:Least-Squares Analysis; D059625:Liquid-Liquid Extraction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014422:Turkeys / Q000097:blood / Q000378:metabolism*
chemical_list: D000871:Anthelmintics; C027187:albendazole sulfone; D015766:Albendazole; C027186:albendazole sulfoxide