title: [Protective effect of astragalosides on anoxia/reoxygenation injury of hippocampal neuron].
abstract: OBJECTIVE
  To study the effect of astragalosides (AST) on the anoxia/reoxygenation (A/R) injured neuron in rat.
  
  
  METHODS
  Primary cultured rat's hippocampal neurons were made into A/R model cells. The cell viability was detected by MTT assay and lactate dehydrogenase releasing methods; the activity of superoxide dismutase (SOD), and contents of malondialdehyde (MDA) and nitride oxide (NO) in culture supernate were detected; the apoptosis rate of hippocampal neurons after A/R was measured by flow cytometry with double-staining of Hoechst33258 and AnnexinV-PI; and intracellular calcium ion [Ca2+]i was observed with a cofocal laser-scanning microscope and determined by fluorescent probe Fluo-3/AM.
  
  
  RESULTS
  AST enhanced the cell viability of neurons after A/R injury, increased SOD activity and decreased the MDA and NO contents in supernate, reduced the A/R-induced apoptosis and decreased the calcium overload in neurons.
  
  
  CONCLUSIONS
  AST has the protective effects on A/R injured neurons, the mechanism is possibly related with its anti-oxidation and calcium overload reducing actions.
authors.affiliation: Department of Pharmacology, Anhui Medical University, Hefei.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Yin
authors.forename: Fen-Fang
authors.initials: FF
authors.lastname: Zhu
authors.forename: Guo-Cui
authors.initials: GC
authors.lastname: Wu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia / Q000187:drug effects; D005260:Female; D005333:Fetus; D006624:Hippocampus / Q000166:cytology*; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D011247:Pregnancy; D061251:Primary Cell Culture / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D012503:Saponins / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D018696:Neuroprotective Agents; D012503:Saponins; D014315:Triterpenes; D009569:Nitric Oxide; C052064:astragaloside A; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D002118:Calcium