title: Evaluation of the naturally acquired antibody immune response to the Pv200L N-terminal fragment of Plasmodium vivax merozoite surface protein-1 in four areas of the Amazon Region of Brazil.
abstract: Frequency and levels of IgG antibodies to an N-terminal fragment of the Plasmodium vivax MSP-1 (Pv200L) protein, in individuals naturally exposed to malaria in four endemic areas of Brazil, were evaluated by enzyme-linked immunosorbent assay. Plasma samples of 261 P. vivax-infected individuals from communities of Macapá, Novo Repartimento, Porto Velho, and Plácido de Castro in the Amazonian region with different malaria transmission intensities. A high mean number of studied individuals (89.3%) presented with antibodies to the Pv200L that correlated with the number of previous malaria infections; there were significant differences in the frequency of the responders (71.9-98.7) and in the antibody levels (1:200-1:51,200) among the four study areas. Results of this study provide evidence that Pv200L is a naturally immunogenic fragment of the PvMSP-1 and is associated with the degree of exposure to parasites. The fine specificity of antibodies to Pv200L is currently being assessed.
authors.affiliation: University of São Paulo State Júlio Mesquita Filho, São José do Rio Preto, São Paulo State, Brazil. stortilu@yahoo.com.br
authors.forename: Luciane M
authors.initials: LM
authors.lastname: Storti-Melo
authors.forename: Wanessa C
authors.initials: WC
authors.lastname: Souza-Neiras
authors.forename: Gustavo C
authors.initials: GC
authors.lastname: Cassiano
authors.forename: Leonardo C
authors.initials: LC
authors.lastname: Taveira
authors.forename: Antônio J
authors.initials: AJ
authors.lastname: Cordeiro
authors.forename: Vanja S C A
authors.initials: VS
authors.lastname: Couto
authors.forename: Marinete M
authors.initials: MM
authors.lastname: Póvoa
authors.forename: Maristela G
authors.initials: MG
authors.lastname: Cunha
authors.forename: Diana M
authors.initials: DM
authors.lastname: Echeverry
authors.forename: Andréa R B
authors.initials: AR
authors.lastname: Rossit
authors.forename: Myriam
authors.initials: M
authors.lastname: Arévalo-Herrera
authors.forename: Sócrates
authors.initials: S
authors.lastname: Herrera
authors.forename: Ricardo L D
authors.initials: RL
authors.lastname: Machado
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D016780:Malaria, Vivax / Q000453:epidemiology* / Q000276:immunology / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D020066:Merozoite Surface Protein 1