title: Evaluation of the prognostic significance of BCL6 gene expression in canine high-grade B-cell lymphoma.
abstract: The clinical usefulness of BCL6 gene expression was evaluated as a prognostic indicator in dogs with high-grade B-cell lymphoma. Forty-four dogs were diagnosed with centroblastic or B-cell immunoblastic type lymphoma according to the updated Kiel classification. BCL6 mRNA expression was measured by real-time PCR and its relationship with prognosis was analyzed. Progression-free and overall survival was not significantly different between the high BCL6 expression group (higher than the median) and the low BCL6 expression group (lower than the median) (P=0.99 and P=0.61, respectively). No correlation between BCL6 and prognosis was observed in this study, which is inconsistent with findings reported for human diffuse large B-cell lymphoma. BCL6 protein expression was not detected in the 11 dogs evaluated by immunohistochemistry. Furthermore, BCL6 protein expression was assessed in 13 archived paraffin-embedded high-grade canine lymphoma tissues and all were also negative. The results suggest that most canine high-grade B-cell lymphomas correspond to human diffuse large B-cell lymphoma with no immunohistochemical expression of BCL6.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sato
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanemoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kagawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D018572:Disease-Free Survival; D004283:Dog Diseases / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000401:mortality / Q000662:veterinary*; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000401:mortality / Q000662:veterinary*; D009367:Neoplasm Staging; D011379:Prognosis; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D051560:Proto-Oncogene Proteins c-bcl-6; D012333:RNA, Messenger