title: [Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody].
abstract: OBJECTIVE
  Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus.
  
  
  METHODS
  Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0.2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions.
  
  
  RESULTS
  The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0.1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus.
  
  
  CONCLUSIONS
  This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing, which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.
authors.affiliation: Chinese Academy of Inspection and Quarantee, Beijing 100025, China.
authors.forename: Kong-xin
authors.initials: KX
authors.lastname: Hu
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Rui-jin
authors.initials: RJ
authors.lastname: Ping
authors.forename: Xue-zheng
authors.initials: XZ
authors.lastname: Ma
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: Yang
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry*; D002642:Chick Embryo; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011485:Protein Binding; D014759:Viral Envelope Proteins / Q000737:chemistry*; D014771:Virion / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40