title: Drosophila lacking a homologue of mammalian ALDH2 have multiple fitness defects.
abstract: Little is known about the roles of aldehyde dehydrogenases in non-vertebrate animals. We recently showed that in Drosophila melanogaster, an enzyme with ∼70% amino acid identity to mammalian ALDH2 is necessary for detoxification of dietary ethanol. To investigate other functions of this enzyme, DmALDH, encoded by the gene Aldh, we compared two strains homozygous for Aldh-null mutations to two closely related wild type strains in measures of fitness and stress resistance in the absence of ethanol. Aldh-null strains have lower total reproductive rate, pre-adult viability, resistance to starvation, and possibly longevity than wild-type strains. When maintained under hyperoxia, Aldh nulls die more quickly and accumulate higher levels of protein carbonyls than wild-types, thereby providing evidence that DmALDH is important for detoxifying reactive aldehydes generated by lipid peroxidation. However no effect of Aldh was seen on protein carbonyl levels in flies maintained under normoxia. It is possible that Aldh nulls experience elevated rates of protein carbonylation under normoxia, but this is compensated (at a fitness cost) by increased rates of degradation of the defective proteins. Alternatively, the fitness defects of Aldh nulls under normoxia may result from the absence of one or more other functions of DmALDH, unrelated to protection against protein carbonylation.
authors.affiliation: Department of Biology, University of Rochester, Rochester, NY 14627-0211, USA. mahulchakraborty@rochester.edu
authors.forename: Mahul
authors.initials: M
authors.lastname: Chakraborty
authors.forename: James D
authors.initials: JD
authors.lastname: Fry
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology*; D005260:Female; D018496:Hyperoxia / Q000201:enzymology / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000235:genetics; D008136:Longevity / Q000235:genetics / Q000502:physiology; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism; D050050:Protein Carbonylation / Q000235:genetics; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013217:Starvation / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology
chemical_list: D024101:Mitochondrial Proteins; D000444:Aldehyde Dehydrogenase