title: Bub1-mediated adaptation of the spindle checkpoint.
abstract: During cell division, the spindle checkpoint ensures accurate chromosome segregation by monitoring the kinetochore-microtubule interaction and delaying the onset of anaphase until each pair of sister chromosomes is properly attached to microtubules. The spindle checkpoint is deactivated as chromosomes start moving toward the spindles in anaphase, but the mechanisms by which this deactivation and adaptation to prolonged mitotic arrest occur remain obscure. Our results strongly suggest that Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest.
authors.affiliation: Center for Childhood Cancer, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, United States of America.
authors.forename: Greicy H
authors.initials: GH
authors.lastname: Goto
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Mishra
authors.forename: Rashid
authors.initials: R
authors.lastname: Abdulle
authors.forename: Clive A
authors.initials: CA
authors.lastname: Slaughter
authors.forename: Katsumi
authors.initials: K
authors.lastname: Kitagawa
mesh_terms: D000705:Anaphase / Q000235:genetics / Q000502:physiology; D017489:CDC28 Protein Kinase, S cerevisiae / Q000235:genetics / Q000502:physiology*; D020090:Chromosome Segregation; D016193:G1 Phase; D018816:Genes, cdc; D018386:Kinetochores / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D010766:Phosphorylation / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D008941:Spindle Apparatus / Q000235:genetics*; D013912:Threonine / Q000235:genetics / Q000378:metabolism
chemical_list: D013912:Threonine; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases; D017489:CDC28 Protein Kinase, S cerevisiae