title: Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?
abstract: There is a growing interest for the application of biomakers to field-collected earthworms. Therefore we have evaluated the usability of native populations of endogeic, widely distributed earthworm Aporrectodea caliginosa in the assessment of soil genotoxicity using the Comet assay. Validation of the Comet assay on earthworm coelomocytes has been established using commercially available Eisenia fetida exposed to copper, cadmium, and pentachlorophenol, along with A. caliginosa exposed to copper in a filter paper contact test. Neutral red retention time (NRRT) assay was conducted on copper exposed and field-collected earthworms. Significant DNA and lysosomal damage was measured using Comet and NRRT assays in native populations of A. caliginosa sampled from the polluted soils in the urban area in comparison to the earthworms from the reference site. The results of this study confirm the employment of A. caliginosa as a suitable species for the in situ soil toxicity and genotoxicity field surveys.
authors.affiliation: Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia. gklobuca@zg.biol.pmf.hr
authors.forename: Göran I V
authors.initials: GI
authors.lastname: Klobučar
authors.forename: Anamaria
authors.initials: A
authors.lastname: Stambuk
authors.forename: Maja
authors.initials: M
authors.lastname: Srut
authors.forename: Ivana
authors.initials: I
authors.lastname: Husnjak
authors.forename: Martina
authors.initials: M
authors.lastname: Merkaš
authors.forename: Luka
authors.initials: L
authors.lastname: Traven
authors.forename: Zelimira
authors.initials: Z
authors.lastname: Cvetković
mesh_terms: D000818:Animals; D001681:Biological Assay; D002104:Cadmium / Q000633:toxicity; D020552:Comet Assay / Q000379:methods*; D003300:Copper / Q000633:toxicity; D017523:Croatia; D004784:Environmental Monitoring / Q000379:methods*; D009499:Neutral Red; D009835:Oligochaeta / Q000187:drug effects*; D010416:Pentachlorophenol / Q000633:toxicity; D012989:Soil Pollutants / Q000633:toxicity*; D001335:Vehicle Emissions / Q000633:toxicity
chemical_list: D012989:Soil Pollutants; D001335:Vehicle Emissions; D002104:Cadmium; D009499:Neutral Red; D003300:Copper; D010416:Pentachlorophenol