title: Two methods of temperature control for single-molecule measurements.
abstract: Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.
authors.affiliation: Clarendon Laboratory, Oxford University, UK.
authors.forename: Matthew A B
authors.initials: MA
authors.lastname: Baker
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Takeda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ishijima
authors.forename: Richard M
authors.initials: RM
authors.lastname: Berry
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002138:Calibration; D004926:Escherichia coli / Q000166:cytology; D005407:Flagella / Q000378:metabolism; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D024462:Myosin Type II / Q000378:metabolism; D011817:Rabbits; D013696:Temperature; D019415:Torque
chemical_list: D001426:Bacterial Proteins; D024462:Myosin Type II