title: Cloning of a gene encoding β-glucosidase from Chaetomium thermophilum CT2 and its expression in Pichia pastoris.
abstract: A new thermostable β-glucosidase gene (bgl) from Chaetomium thermophilum CT2 was cloned, sequenced and expressed. The full-length DNA of bgl was 3,101 bp and included three introns. The full-length cDNA contained an open reading frame of 2,604-bp nucleotides, encoding 867 amino acids with a potential secretion signal. The C. thermophilum CT2 β-glucosidase gene was functionally expressed in Pichia pastoris. The purified recombinant β-glucosidase was a 119-kDa glycoprotein with an optimum catalytic activity at pH 5.0 and 60°C. The enzyme was stable at 50°C, and retained 67.7% activity after being kept at 60°C for 1 h; the half-time of the enzyme at 65°C was approximately 55 min, and even retained 29.7% activity after incubation at 70°C for 10 min.
authors.affiliation: Department of Environmental Biology, Shandong Agricultural University, Tai'an, China.
authors.forename: Rongyan
authors.initials: R
authors.lastname: Xu
authors.forename: Fangchao
authors.initials: F
authors.lastname: Teng
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Duochuan
authors.initials: D
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D002597:Chaetomium / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D007438:Introns; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D010843:Pichia / Q000235:genetics*; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D001617:beta-Glucosidase