title: Caprine (goat) collagen: a potential biomaterial for skin tissue engineering.
abstract: Collagens presently used in tissue engineering are primarily of bovine or porcine origin. However, a risk of a spongiform encephalopathy epidemic has limited the use of collagen from these sources. Keeping the aforementioned perspective in mind, we explored the possibility of using  domestic goat available in the subcontinent as a potential source of collagen for tissue-engineering application. This article delineates the isolation, physico-chemical characterization, biocompatibility study and wound healing application of acid soluble caprine (goat) tendon collagen (GTC).  Physico-chemical characterization of 1% acetic acid extracted GTC was done by SDS-PAGE, amino-acid composition analysis, FT-IR and CD spectroscopy. Results revealed that GTC was comprised of type-I collagen. Biocompatibility study showed that GTC augmented cell adhesion, cell cycle progression  and proliferation. Immuno-cytochemical analysis in conjugation with traction force microscopy further confirmed a superior focal adhesion complex mediated cell-substrate interaction in GTC. Finally, in vivo study in mice model revealed that GTC has low immunogenicity and it augments  healing process significantly. Throughout the study, calf skin collagen (CSC) was used as standard for comparative evaluation. In conclusion, it can be said that GTC may find its application as biomaterial in skin tissue engineering.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, India.
authors.forename: Indranil
authors.initials: I
authors.lastname: Banerjee
authors.forename: Debasish
authors.initials: D
authors.lastname: Mishra
authors.forename: Tamal
authors.initials: T
authors.lastname: Das
authors.forename: Swatilekha
authors.initials: S
authors.lastname: Maiti
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006041:Goats; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism; D012867:Skin / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D012995:Solubility; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D010750:Phosphoproteins; D003094:Collagen; D051416:Focal Adhesion Protein-Tyrosine Kinases