title: Macrophages from chickens selected for high antibody response produced more nitric oxide and have greater phagocytic capacity.
abstract: Macrophages are fundamental cells of the innate immune system, which, through phagocytosis and nitric oxide production, eliminate pathogens. The aim of the present study was to determine if macrophages from chicken families divergently selected to high and low antibodies response differ in nitric oxide production and phagocytic capacity. Blood monocytes derived macrophages were activated with lipopolysaccharide and supernatant from chicken spleen lymphocytes cultured with Concanavalin A (containing chicken interferon). Nitric oxide production was evaluated in culture supernatants. Phagocytic capacity of activated and non-activated macrophages was assayed using yeasts and IgY opsonized sheep red blood cells. Activated and non-activated macrophages from the high antibodies response family produced higher nitric oxide levels, internalized more yeast and significantly more opsonized sheep red blood cells than macrophages from the low antibodies response family. Moreover, activated macrophages became more elongated and widely spread. These findings indicate that macrophages from the high antibodies response family were more active suggesting that the differences in antibody response also depend on macrophage function.
authors.affiliation: Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil.
authors.forename: Marco Cesar Cunegundes
authors.initials: MC
authors.lastname: Guimarães
authors.forename: Landi Veivi Costilla
authors.initials: LV
authors.lastname: Guillermo
authors.forename: Marcos Fernando de Rezende
authors.initials: MF
authors.lastname: Matta
authors.forename: Sandro Gomes
authors.initials: SG
authors.lastname: Soares
authors.forename: Renato Augusto
authors.initials: RA
authors.lastname: DaMatta
mesh_terms: D000818:Animals; D000917:Antibody Formation; D048430:Cell Shape; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology* / Q000378:metabolism*; D007113:Immunity, Innate; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis
chemical_list: D009569:Nitric Oxide