title: Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase gene.
abstract: Lilium cv Acapulco was transformed with a defective cucumber mosaic virus (CMV) replicase gene (CMV2-GDD) construct using Agrobacterium tumefaciens. Four lines were analyzed for gene expression and resistance to CMV-O strain. Expression of the CMV2-GDD gene in the transgenic plants was confirmed by reverse transcription PCR (RT-PCR). When these four lines were mechanically inoculated with CMV-O, no signal of coat protein (CP) messages using RT-PCR was detected in newly produced leaves of two transgenic lines. Dot-immunobinding assay (DIBA) of CP was performed to examine the presence of the CMV in the newly produced leaves of challenged plants. Results, similar to those obtained with RT-PCR of the CP messages, were observed in DIBA. Therefore, our results imply that the two lines show increased levels of resistance to CMV, and CMV-GDD replicase gene is an effective construct that has protection against CMV in Lilium.
authors.affiliation: Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba, 271-8510, Japan.
authors.forename: Pejman
authors.initials: P
authors.lastname: Azadi
authors.forename: Ntui Valentaine
authors.initials: NV
authors.lastname: Otang
authors.forename: Hasthanasombut
authors.initials: H
authors.lastname: Supaporn
authors.forename: Raham Sher
authors.initials: RS
authors.lastname: Khan
authors.forename: Dong Poh
authors.initials: DP
authors.lastname: Chin
authors.forename: Ikuo
authors.initials: I
authors.lastname: Nakamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mii
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D001483:Base Sequence; D017799:Cucumovirus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity*; D004279:DNA, Viral / Q000235:genetics; D003673:Defective Viruses / Q000201:enzymology / Q000235:genetics; D005814:Genes, Viral; D027762:Lilium / Q000235:genetics* / Q000821:virology*; D010935:Plant Diseases / Q000235:genetics / Q000517:prevention & control / Q000821:virology; D030821:Plants, Genetically Modified; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004279:DNA, Viral; D012324:RNA-Dependent RNA Polymerase