title: Gene silencing of Tead3 abrogates radiation-induced adaptive response in cultured mouse limb bud cells.
abstract: There is a crucial need to better understand the effects of low-doses of ionizing radiation in fetal models. Radiation-induced adaptive response (AR) was described in mouse embryos pre-exposed in utero to low-doses of X-rays, which exhibited lower apoptotic levels in the limb bud. We previously described AR-specific gene modulations in the mouse embryo. In this study, we evaluated the role of three candidate genes in the apoptotic AR in a micromass culture of limb bud cells: Csf1, Cacna1a and Tead3. Gene silencing of these three genes abrogated AR. Knowing that TEAD3 protein levels are significantly higher in adapted cells and that YAP/TAZ/TEAD are involved in the control of cell proliferation and apoptosis, we suggest that modulation of Tead3 could play a role in the induction of AR in our model, seen as a reduction of radiation-induced apoptosis and a stimulation of proliferation and differentiation in limb bud cells.
authors.affiliation: National Institute of Radiological Sciences, Chiba, Japan. vares@nirs.go.jp
authors.forename: Guillaume
authors.initials: G
authors.lastname: Vares
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yi
authors.initials: Y
authors.lastname: Shang
authors.forename: Keiko
authors.initials: K
authors.lastname: Taki
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakajima
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nenoi
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology / Q000528:radiation effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D020868:Gene Silencing / Q000502:physiology; D018878:Limb Buds / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D011836:Radiation Tolerance / Q000502:physiology* / Q000528:radiation effects; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000091262:TEA Domain Transcription Factors; C432156:Tead3 protein, mouse; D014157:Transcription Factors