title: Shrimp waste fermentation with Pseudomonas aeruginosa A2: optimization of chitin extraction conditions through Plackett-Burman and response surface methodology approaches.
abstract: A Box-Bhenken design with four variables (shrimp shell concentration (SSC), glucose concentration, incubation time and inoculum size) and three levels was used for the determination of the deproteinization and demineralization efficiencies in fermented shrimp shells by Pseudomonas aeruginosa A2. The fermentation variables were selected in accordance with Plackett-Burman design. Maximum demineralization of 96%, with about 89% of protein removal occurs under the following conditions: SSC 50 g/l, glucose 50 g/l, 5 days and inoculum of 0.05 OD. This environment friendly method (biological treatment) can be considered as an effective pretreatment to produce a high-quality chitin.
authors.affiliation: Université de Sfax, Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, Route Soukra km 3.5, BP1173 - 3038 Sfax, Tunisia. olfa_ghor@yahoo.fr
authors.forename: Olfa
authors.initials: O
authors.lastname: Ghorbel-Bellaaj
authors.forename: Noomen
authors.initials: N
authors.lastname: Hmidet
authors.forename: Kemel
authors.initials: K
authors.lastname: Jellouli
authors.forename: Islem
authors.initials: I
authors.lastname: Younes
authors.forename: Hana
authors.initials: H
authors.lastname: Maâlej
authors.forename: Ridha
authors.initials: R
authors.lastname: Hachicha
authors.forename: Moncef
authors.initials: M
authors.lastname: Nasri
mesh_terms: D000825:Animal Structures / Q000033:anatomy & histology / Q000737:chemistry*; D000818:Animals; D002686:Chitin / Q000032:analysis / Q000302:isolation & purification*; D005285:Fermentation; D005398:Fisheries; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D033561:Penaeidae / Q000033:anatomy & histology*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D014866:Waste Products
chemical_list: D014866:Waste Products; D002686:Chitin