title: CDK11(p58) is required for centriole duplication and Plk4 recruitment to mitotic centrosomes.
abstract: BACKGROUND
  CDK11(p58) is a mitotic protein kinase, which has been shown to be required for different mitotic events such as centrosome maturation, chromatid cohesion and cytokinesis.
  
  
  RESULTS
  In addition to these previously described roles, our study shows that CDK11(p58) inhibition induces a failure in the centriole duplication process in different human cell lines. We propose that this effect is mediated by the defective centrosomal recruitment of proteins at the onset of mitosis. Indeed, Plk4 protein kinase and the centrosomal protein Cep192, which are key components of the centriole duplication machinery, showed reduced levels at centrosomes of mitotic CDK11-depleted cells. CDK11(p58), which accumulates only in the vicinity of mitotic centrosomes, directly interacts with the centriole-associated protein kinase Plk4 that regulates centriole number in cells. In addition, we show that centriole from CDK11 defective cells are not able to be over duplicated following Plk4 overexpression.
  
  
  CONCLUSIONS
  We thus propose that CDK11 is required for centriole duplication by two non-mutually-exclusive mechanisms. On one hand, the observed duplication defect could be caused indirectly by a failure of the centrosome to fully maturate during mitosis. On the other hand, CDK11(p58) could also directly regulate key centriole components such as Plk4 during mitosis to trigger essential mitotic centriole modifications, required for centriole duplication during subsequent interphase.
authors.affiliation: UMR6061-CNRS, Institut de Génétique et Développement de Rennes, Université de Rennes I, Rennes, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Franck
authors.forename: Emilie
authors.initials: E
authors.lastname: Montembault
authors.forename: Pierre
authors.initials: P
authors.lastname: Romé
authors.forename: Aude
authors.initials: A
authors.lastname: Pascal
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Cremet
authors.forename: Régis
authors.initials: R
authors.lastname: Giet
mesh_terms: D002502:Centrioles / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D056743:Cyclin D3 / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport
chemical_list: C534696:CCND3 protein, human; D056743:Cyclin D3; C490219:PLK4 protein, human; D017346:Protein Serine-Threonine Kinases