title: Directed in vivo angiogenesis assay and the study of systemic neoangiogenesis in cancer.
abstract: Targeting neoangiogenesis is a well-established anticancer strategy, however, one of the major problems in angiogenesis research, both at the basic and applied levels, remains the development of suitable in vivo methods for assessing and quantifying the systemic angiogenic response. Therefore, there is an urgent need to adopt technically simple and reproducible methodologies which allow to easily quantify neoangiogenesis independently of morphological parameters. Recently, a reproducible and quantitative method was developed, the directed in vivo angiogenesis assay (DIVAA) consisting of the subcutaneous implantation of surgical grade silicone cylinders closed at one end, called angioreactors, into the dorsal flanks of nude mice. In the past few years, DIVAA has been successfully used in evaluating the inhibition and or enhancement of systemic perturbation of angiogenesis by several molecules. Thus, DIVAA studies systemic angiogenesis and its therapeutic modulation associated to cancer progression and metastasis.
authors.affiliation: Department of General Pathology, 1st School of Medicine, II University of Naples, Naples, Italy. claudio.napoli@unina2.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Napoli
authors.forename: Antonio
authors.initials: A
authors.lastname: Giordano
authors.forename: Amelia
authors.initials: A
authors.lastname: Casamassimi
authors.forename: Francesca
authors.initials: F
authors.lastname: Pentimalli
authors.forename: Louis J
authors.initials: LJ
authors.lastname: Ignarro
authors.forename: Filomena
authors.initials: F
authors.lastname: De Nigris
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D035843:Biomedical Research / Q000639:trends; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D008495:Medical Oncology / Q000379:methods; D051379:Mice; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000473:pathology*; D009389:Neovascularization, Pathologic; D015398:Signal Transduction
chemical_list: D000970:Antineoplastic Agents