title: eIF2α kinases control chalone production in Dictyostelium discoideum.
abstract: Growing Dictyostelium cells secrete CfaD and AprA, two proteins that have been characterized as chalones. They exist within a high-molecular-weight complex that reversibly inhibits cell proliferation, but not growth, via cell surface receptors and a signaling pathway that includes G proteins. How the production of these two proteins is regulated is unknown. Dictyostelium cells possess three GCN2-type eukaryotic initiation factor 2 α subunit (eIF2α) kinases, proteins that phosphorylate the translational initiation factor eIF2α and possess a tRNA binding domain involved in their regulation. The Dictyostelium kinases have been shown to function during development in regulating several processes. We show here that expression of an unregulated, activated kinase domain greatly inhibits cell proliferation. The inhibitory effect on proliferation is not due to a general inhibition of translation. Instead, it is due to enhanced production of a secreted factor(s). Indeed, extracellular CfaD and AprA proteins, but not their mRNAs, are overproduced in cells expressing the activated kinase domain. The inhibition of proliferation is not seen when the activated kinase domain is expressed in cells lacking CfaD or AprA or in cells that contain a nonphosphorylatable eIF2α. We conclude that production of the chalones CfaD and AprA is translationally regulated by eIF2α phosphorylation. Both proteins are upregulated at the culmination of development, and this enhanced production is lacking in a strain that possesses a nonphosphorylatable eIF2α.
authors.affiliation: Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235-1634, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Bowman
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Xiong
authors.forename: Janet H
authors.initials: JH
authors.lastname: Kirsten
authors.forename: Charles K
authors.initials: CK
authors.lastname: Singleton
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D050150:Chalones / Q000096:biosynthesis*; D017077:Culture Media, Conditioned / Q000378:metabolism; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D050150:Chalones; D017077:Culture Media, Conditioned; D015852:Eukaryotic Initiation Factor-2; D015800:Protozoan Proteins