title: [Expression and crystallographic studies of a fungal immunomodulatory protein LZ-8 from a medicinal fungus Ganoderma lucidum].
abstract: LZ-8 protein, isolated from a well known Chinese traditional medicinal fungus Ganoderma lucidum, is the first member of fungal immunomodulatory protein, members of which have been isolated from a variety of medicinal and edible mushrooms in the last two decades. The protein plays a multifunctional and important role in modulating immune system. In this report, in order to get LZ-8 protein crystals, the LZ-8 gene was expressed and purified by affinity chromatography, gel filtration chromatography and anion exchange chromatography subsequently. The protein was then crystallized using the hanging-drop vapour-diffusion method. The LZ-8 crystals were obtained and the phase information was calculated by X-ray diffraction. The resolution of LZ-8 crystals is 3.2A. This study will provide an insight into the structure of fungal immunomodulatory proteins.
authors.affiliation: Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Min
authors.initials: M
authors.lastname: An
authors.forename: George Fu
authors.initials: GF
authors.lastname: Gao
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D003461:Crystallography; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D032902:Ganoderma / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D007155:Immunologic Factors / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
chemical_list: D005656:Fungal Proteins; D007155:Immunologic Factors; D011994:Recombinant Proteins; C061148:LZ-8 protein, Ganoderma lucidum