title: Procathepsin D and cytokines influence the proliferation of lung cancer cells.
abstract: Enzymatically inactive procathepsin D (pCD) has been established as an important factor in the development of lung cancer. In addition to serving as a growth factor, pCD is also involved in communication between cancer cells and surrounding cells. In the present investigation, a possible combination of autocrine and paracrine actions of pCD was investigated. pCD initiated secretion of interleukin (IL)-4, IL-8, IL-10 and IL-13 from lung cancer cells. These cytokines participated in the proliferation of the cancer cells, as demonstrated both by adding the cytokines and by inhibition using specific anti-cytokine antibodies. Using several clones of lung cancer cells differing in production of activation peptide, the pCD/cytokine cooperation and action was shown to be dependent on pCD secretion. Further proof that pCD is one of the key molecules involved in cancer development is presented.
authors.affiliation: Department of Pathology, University of Louisville, Louisville, KY, USA. vaclav.vetvicka@louisville.edu
authors.forename: Vaclav
authors.initials: V
authors.lastname: Vetvicka
authors.forename: Jana
authors.initials: J
authors.lastname: Vetvickova
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002402:Cathepsin D / Q000378:metabolism*; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D016207:Cytokines; D004792:Enzyme Precursors; D018793:Interleukin-13; D016209:Interleukin-8; D016753:Interleukin-10; D015847:Interleukin-4; C058345:procathepsin D; D002402:Cathepsin D