title: Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin β.
abstract: We characterize the previously unrecognized phenomenon of axotomy-induced axonogenesis in rat embryonic hippocampal neurons in vitro and elucidate the underlying mechanism. New neurites arose from cell bodies after axotomy and grew. These neurites were Tau-1-positive, and the injured axons showed negative immunoreactivity for Tau-1. Axonogenesis was delayed in these neurons by inhibiting the dynein-dynactin complex through the overexpression of p50. Importin β, which was locally translated after axotomy, was associated with the dynein-importin α complex and was required for axonogenesis. Taken together, these results suggest that retrograde transport of injury-induced signals in injured axons play key roles in the axotomy-induced axonogenesis of hippocampal neurons.
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ohara
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hata
authors.forename: Noriko
authors.initials: N
authors.lastname: Yasuhara
authors.forename: Rashid
authors.initials: R
authors.lastname: Mehmood
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoneda
authors.forename: Masanori
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Toshihide
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019771:Axotomy; D002478:Cells, Cultured; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism; D006624:Hippocampus / Q000293:injuries* / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016501:Neurites / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D028961:beta Karyopherins / Q000378:metabolism*
chemical_list: D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D028961:beta Karyopherins; D004398:Dyneins