title: Microgenomics of ameloblastoma.
abstract: Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.
authors.affiliation: Department of Pathology, University of Alabama at Birmingham, USA.
authors.forename: P
authors.initials: P
authors.lastname: DeVilliers
authors.forename: C
authors.initials: C
authors.lastname: Suggs
authors.forename: D
authors.initials: D
authors.lastname: Simmons
authors.forename: V
authors.initials: V
authors.lastname: Murrah
authors.forename: J T
authors.initials: JT
authors.lastname: Wright
mesh_terms: D000564:Ameloblastoma / Q000235:genetics*; D053523:Amelogenin / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D064032:Calbindin 2; D003746:Dental Enamel Proteins / Q000235:genetics; D016326:Extracellular Matrix Proteins; D020869:Gene Expression Profiling / Q000379:methods; D023281:Genomics / Q000379:methods*; D053823:Hedgehog Proteins / Q000235:genetics; D006801:Humans; D007610:Kallikreins / Q000235:genetics; D054023:Lasers, Semiconductor; D053519:Matrix Metalloproteinase 20 / Q000235:genetics; D042282:Microdissection / Q000379:methods; D009363:Neoplasm Proteins / Q000235:genetics; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D000072079:Patched Receptors; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D064030:S100 Calcium Binding Protein G / Q000235:genetics; D000072086:Smoothened Receptor; D051153:Wnt Proteins / Q000235:genetics
chemical_list: C490889:AMBN protein, human; C507199:AMELX protein, human; D053523:Amelogenin; D014408:Biomarkers, Tumor; D064032:Calbindin 2; D003746:Dental Enamel Proteins; C550525:ENAM protein, human; D016326:Extracellular Matrix Proteins; D053823:Hedgehog Proteins; D009363:Neoplasm Proteins; D000072079:Patched Receptors; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D064030:S100 Calcium Binding Protein G; C509239:SHH protein, human; C491023:SMO protein, human; D000072086:Smoothened Receptor; C484142:WNT10A protein, human; D051153:Wnt Proteins; D007610:Kallikreins; C120801:kallikrein 4; C507111:MMP20 protein, human; D053519:Matrix Metalloproteinase 20