title: Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.
abstract: In Schizosaccharomyces pombe, the RNAi pathway is required for the formation of pericentric heterochromatin, proper chromosome segregation, and repression of pericentric meiotic recombination. Here we demonstrate that, when the activity of the histone H3 Lys 14 (H3K14) acetyltransferase Mst2 is eliminated, the RNAi machinery is no longer required for pericentric heterochromatin functions. We further reveal that reducing RNA polymerase II recruitment to pericentric regions is essential for maintaining heterochromatin in the absence of RNAi.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
authors.forename: Bharat D
authors.initials: BD
authors.lastname: Reddy
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Lifang
authors.initials: L
authors.lastname: Niu
authors.forename: Emily C
authors.initials: EC
authors.lastname: Higuchi
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Marguerat
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
authors.forename: Gerald R
authors.initials: GR
authors.lastname: Smith
authors.forename: Songtao
authors.initials: S
authors.lastname: Jia
mesh_terms: D002503:Centromere / Q000378:metabolism; D006570:Heterochromatin / Q000378:metabolism*; D051548:Histone Acetyltransferases / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology*; D012319:RNA Polymerase II / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D006570:Heterochromatin; D029702:Schizosaccharomyces pombe Proteins; D051548:Histone Acetyltransferases; D012319:RNA Polymerase II