title: The low resolution structure of ApoA1 in spherical high density lipoprotein revealed by small angle neutron scattering.
abstract: Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering with contrast variation was used to determine the solution structure of protein and lipid components of reconstituted sHDL. Apolipoprotein A1, the major protein of sHDL, forms a hollow structure that cradles a central compact lipid core. Three apoA1 chains are arranged within the low resolution structure of the protein component as one of three possible global architectures: (i) a helical dimer with a hairpin (HdHp), (ii) three hairpins (3Hp), or (iii) an integrated trimer (iT) in which the three apoA1 monomers mutually associate over a portion of the sHDL surface. Cross-linking and mass spectrometry analyses help to discriminate among the three molecular models and are most consistent with the HdHp overall architecture of apoA1 within sHDL.
authors.affiliation: Department of Cell Biology, Center for Cardiovascular Diagnostics and Prevention, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wu
authors.forename: Valentin
authors.initials: V
authors.lastname: Gogonea
authors.forename: Xavier
authors.initials: X
authors.lastname: Lee
authors.forename: Roland P
authors.initials: RP
authors.lastname: May
authors.forename: Vitaliy
authors.initials: V
authors.lastname: Pipich
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Wagner
authors.forename: Arundhati
authors.initials: A
authors.lastname: Undurti
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Tallant
authors.forename: Camelia
authors.initials: C
authors.lastname: Baleanu-Gogonea
authors.forename: Francesca
authors.initials: F
authors.lastname: Charlton
authors.forename: Alexander
authors.initials: A
authors.lastname: Ioffe
authors.forename: Joseph A
authors.initials: JA
authors.lastname: DiDonato
authors.forename: Kerry-Anne
authors.initials: KA
authors.lastname: Rye
authors.forename: Stanley L
authors.initials: SL
authors.lastname: Hazen
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry*; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry*; D013058:Mass Spectrometry; D009502:Neutrons; D055503:Protein Multimerization; D053838:Scattering, Small Angle
chemical_list: D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL