title: DNA microarray profiling identified a new role of growth hormone in vascular remodeling of rat ductus arteriosus.
abstract: The ductus arteriosus (DA), a fetal arterial connection between the pulmonary artery and the aorta, has a character distinct from the adjacent arteries. We compared the transcriptional profiles of the DA and the aorta of Wistar rat fetuses on embryonic day 19 (preterm) and day 21 (near-term) using DNA microarray analyses. We found that 39 genes were expressed 2.5-fold greater in the DA than in the aorta. Growth hormone (GH) receptor (GHR) exhibited the most significant difference in expression. Then, we found that GH significantly promoted migration of DA smooth muscle cells (SMCs), thus enhancing the intimal cushion formation of the DA explants. GH also regulated the expression of cytoskeletal genes in DA SMCs, which may retain a synthetic phenotype in the smooth muscle-specific cytoskeletal genes. Thus, the present study revealed that GH-GHR signal played a role in the vascular remodeling of the DA.
authors.affiliation: Cardiovascular Research Institute, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Jin
authors.forename: Utako
authors.initials: U
authors.lastname: Yokoyama
authors.forename: Yoji
authors.initials: Y
authors.lastname: Sato
authors.forename: Aki
authors.initials: A
authors.lastname: Shioda
authors.forename: Qibin
authors.initials: Q
authors.lastname: Jiao
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Minamisawa
mesh_terms: D000818:Animals; D001011:Aorta / Q000196:embryology / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D048708:Cell Growth Processes / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000235:genetics; D004373:Ductus Arteriosus / Q000196:embryology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000196:embryology / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D013006:Growth Hormone / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000196:embryology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; D013006:Growth Hormone; C051815:somatotropin-binding protein