title: Anti-mutagenic and pro-apoptotic effects of apigenin on human chronic lymphocytic leukemia cells.
abstract: Diet can play a vital role in cancer prevention. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. The purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. In present study human chronic lymphocytic leukemia (Eheb cell line) were cultured in RPMI 1640 (Sigma), supplemented with 10% fetal calf serum, penicillin-streptomycin, L-glutamine and incubated at 37 ºC for 2 days. In addition cancer cell line was treated by and apigenin and cellular vital capacity was determined by MTT assay. Then effect of apigenin in human lymphoma B cells was examined by flow cytometry techniques. The apigenin was subsequently evaluated in terms of anti-mutagenic properties by a standard reverse mutation assay (Ames test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a foreign supply to ensure its growth. The aforementioned strain gives rise to reverted colonies when exposed to sodium azide as a carcinogen substance. During MTT assay, human chronic lymphocytic leukemia revealed to have a meaningful cell death when compared with controls (P<0.01) Apoptosis was induced suitably after 48 hours by flow cytometry assay. In Ames test apigenin prevented the reverted mutations and the hindrance percent of apigenin was 98.17%.These results have revealed apigenin induced apoptosis in human lymphoma B cells in vitro.
authors.affiliation: Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran. mhashemi@iautmu.ac.ir
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nouri Long
authors.forename: Maliheh
authors.initials: M
authors.lastname: Entezari
authors.forename: Shohreh
authors.initials: S
authors.lastname: Nafisi
authors.forename: Hossein
authors.initials: H
authors.lastname: Nowroozii
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D013997:Time Factors
chemical_list: D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D047310:Apigenin