title: [Differential expression of hsa-miR-126 and hsa-miR-518b in esophageal squamous carcinoma].
abstract: OBJECTIVE
  To identify the differentially expressed microRNAs (miRNAs) between esophageal squamous carcinoma (ESC) and adjacent non-tumorous tissue (NT).
  
  
  METHODS
  The expression levels of the miRNAs were detected in 3 fresh ESC and NT samples by hybridization with miRNAs microarray chip. Real-time quantitative RT-PCR was performed to confirm the results of the microarray analysis. The expressions of hsa-miR-126 and hsa-miR-518b in ESC were validated by real-time quantitative RT-PCR in another independent 15 matched samples.
  
  
  RESULTS
  A total of 11 miRNAs exhibited differential expressions in ESC samples as compared to their expressions in the NT samples, including a 1 up-regulated miRNA and 10 down-regulated miRNAs. Compared with normal esophageal samples, the ESC tissues showed up-regulated hsa-miR-126 and down-regulated hsa-miR-518b expression.
  
  
  CONCLUSIONS
  hsa-miR-126 and hsa-miR-518b are differentially expressed in ESC, and they might play important roles in the carcinogenesis and progression of ESC.
authors.affiliation: Department of Oncology Surgery, Xi an Jiaotong University College of Medicine, Xi an 710061, China. caihui@stu.xjtu.edu.cn
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Wang
authors.forename: Ming-xin
authors.initials: MX
authors.lastname: Zhang
authors.forename: Xiao-yi
authors.initials: XY
authors.lastname: Duan
authors.forename: Ren-qiang
authors.initials: RQ
authors.lastname: Ma
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; C527111:MIRN126 microRNA, human; D035683:MicroRNAs