title: Analysis of expressed sequence tags derived from a compatible Mycosphaerella fijiensis-banana interaction.
abstract: Mycosphaerella fijiensis, a hemibiotrophic fungus, is the causal agent of black leaf streak disease, the most serious foliar disease of bananas and plantains. To analyze the compatible interaction of M. fijiensis with Musa spp., a suppression subtractive hybridization (SSH) cDNA library was constructed to identify transcripts induced at late stages of infection in the host and the pathogen. In addition, a full-length cDNA library was created from the same mRNA starting material as the SSH library. The SSH procedure was effective in identifying specific genes predicted to be involved in plant-fungal interactions and new information was obtained mainly about genes and pathways activated in the plant. Several plant genes predicted to be involved in the synthesis of phenylpropanoids and detoxification compounds were identified, as well as pathogenesis-related proteins that could be involved in the plant response against M. fijiensis infection. At late stages of infection, jasmonic acid and ethylene signaling transduction pathways appear to be active, which corresponds with the necrotrophic life style of M. fijiensis. Quantitative PCR experiments revealed that antifungal genes encoding PR proteins and GDSL-like lipase are only transiently induced 30 days post inoculation (dpi), indicating that the fungus is probably actively repressing plant defense. The only fungal gene found was induced 37 dpi and encodes UDP-glucose pyrophosphorylase, an enzyme involved in the biosynthesis of trehalose. Trehalose biosynthesis was probably induced in response to prior activation of plant antifungal genes and may act as an osmoprotectant against membrane damage.
authors.affiliation: Instituto de Biotecnología de las Plantas, Universidad Central Marta Abreu de Las Villas, Carretera a Camajuaní km 5.5, 54 830, Santa Clara, Cuba.
authors.forename: Orelvis
authors.initials: O
authors.lastname: Portal
authors.forename: Yovanny
authors.initials: Y
authors.lastname: Izquierdo
authors.forename: David
authors.initials: D
authors.lastname: De Vleesschauwer
authors.forename: Aminael
authors.initials: A
authors.lastname: Sánchez-Rodríguez
authors.forename: Milady
authors.initials: M
authors.lastname: Mendoza-Rodríguez
authors.forename: Mayra
authors.initials: M
authors.lastname: Acosta-Suárez
authors.forename: Bárbara
authors.initials: B
authors.lastname: Ocaña
authors.forename: Elio
authors.initials: E
authors.lastname: Jiménez
authors.forename: Monica
authors.initials: M
authors.lastname: Höfte
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity; D003517:Cyclopentanes / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D054884:Host-Pathogen Interactions / Q000235:genetics*; D028521:Musa / Q000235:genetics* / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013997:Time Factors; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000235:genetics*
chemical_list: D003517:Cyclopentanes; D005030:Ethylenes; D005656:Fungal Proteins; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; C053376:pathogenesis-related proteins, plant; C011006:jasmonic acid; C036216:ethylene; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase