title: VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering.
abstract: We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are identical. Mutant VloxP and SloxP, which have the same uses as mutant loxP, were also developed. VCre/VloxP and SCre/SloxP in combination with Cre/loxP and Flp/FRT systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.
authors.affiliation: Department of Human Genome Research, Kazusa DNA Research Institute, Graduate School of Pharmaceutical Sciences, Chiba University, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Suzuki
authors.forename: Manabu
authors.initials: M
authors.lastname: Nakayama
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D016678:Genome; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D009154:Mutation; D045522:Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D045522:Recombinases; D004247:DNA