title: Vitrified human ovaries have fewer primordial follicles and produce less antimüllerian hormone than slow-frozen ovaries.
abstract: Slow-freezing and vitrification methods of human ovarian tissue cryopreservation were compared in terms of primordial follicle count and in vitro antimüllerian hormone (AMH) and estradiol production. Compared with fresh and slow-frozen ovaries, vitrified ovaries contained statistically significantly fewer primordial follicles and produced statistically significantly less AMH in vitro. Estradiol production from slow-frozen and vitrified ovaries was similar but statistically significantly lower than from fresh cultured strips.
authors.affiliation: Women's Health Center, Assisted Reproduction Unit, American Hospital, Istanbul, Turkey. drozguroktem@gmail.com
authors.forename: Ozgur
authors.initials: O
authors.lastname: Oktem
authors.forename: Ebru
authors.initials: E
authors.lastname: Alper
authors.forename: Basak
authors.initials: B
authors.lastname: Balaban
authors.forename: Erhan
authors.initials: E
authors.lastname: Palaoglu
authors.forename: Kamil
authors.initials: K
authors.lastname: Peker
authors.forename: Cengiz
authors.initials: C
authors.lastname: Karakaya
authors.forename: Bulent
authors.initials: B
authors.lastname: Urman
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D054304:Anti-Mullerian Hormone / Q000378:metabolism*; D015925:Cryopreservation / Q000379:methods*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005615:Freezing; D006801:Humans; D006080:Ovarian Follicle / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D013997:Time Factors; D046509:Tissue Culture Techniques; D058989:Vitrification
chemical_list: D004958:Estradiol; D054304:Anti-Mullerian Hormone