title: AP-site cleavage activity of tyrosyl-DNA phosphodiesterase 1.
abstract: APE-independent base excision repair (BER) pathway plays an important role in the regulation of DNA repair mechanisms. In this study it has been found that recently discovered tyrosyl-DNA phosphodiesterase 1 (Tdp1) catalyzes the AP site cleavage reaction to generate breaks with the 3'- and 5'-phosphate termini. The removal of the 3'-phosphate is performed by polynucleotide kinase phosphatase (PNKP). Tdp1 is known to interact stably with BER proteins: DNA polymerase beta (Pol β), XRCC1, PARP1 and DNA ligase III. The data suggest a role of Tdp1 in the new APE-independent BER pathway in mammals.
authors.affiliation: SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Lebedeva
authors.forename: Nadejda I
authors.initials: NI
authors.lastname: Rechkunova
authors.forename: Olga I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D001483:Base Sequence; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000378:metabolism; D006801:Humans; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D011119:Polynucleotides / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins; D013379:Substrate Specificity
chemical_list: D011119:Polynucleotides; D011994:Recombinant Proteins; C530380:PNKP protein, human; D017853:Phosphotransferases (Alcohol Group Acceptor); D010727:Phosphoric Diester Hydrolases; C498508:TDP1 protein, human; D045647:DNA Glycosylases; C473634:NEIL1 protein, human; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D045643:DNA Repair Enzymes