title: End-functionalized glycopolymers as mimetics of chondroitin sulfate proteoglycans.
abstract: Glycosaminoglycans are sulfated polysaccharides that play important roles in fundamental biological processes, such as cell division, viral invasion, cancer and neuroregeneration. The multivalent presentation of multiple glycosaminoglycan chains on proteoglycan scaffolds may profoundly influence their interactions with proteins and subsequent biological activity. However, the importance of this multivalent architecture remains largely unexplored, and few synthetic mimics exist for probing and manipulating glycosaminoglycan activity. Here, we describe a new class of end-functionalized ring-opening metathesis polymerization (ROMP) polymers that mimic the native-like, multivalent architecture found on chondroitin sulfate (CS) proteoglycans. We demonstrate that these glycopolymers can be readily integrated with microarray and surface plasmon resonance technology platforms, where they retain the ability to interact selectively with proteins. ROMP-based glycopolymers are part of a growing arsenal of chemical tools for probing the functions of glycosaminoglycans and for studying their interactions with proteins.
authors.affiliation: Division of Chemistry and Chemical Engineering and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, 91125, USA. lhw@caltech.edu ; ; Tel: +1 626 395 6101.
authors.forename: Song-Gil
authors.initials: SG
authors.lastname: Lee
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Brown
authors.forename: Claude J
authors.initials: CJ
authors.lastname: Rogers
authors.forename: John B
authors.initials: JB
authors.lastname: Matson
authors.forename: Chithra
authors.initials: C
authors.lastname: Krishnamurthy
authors.forename: Manish
authors.initials: M
authors.lastname: Rawat
authors.forename: Linda C
authors.initials: LC
authors.lastname: Hsieh-Wilson