title: Parameters optimization defined by statistical analysis for cysteine-dextran radiolabeling with technetium tricarbonyl core.
abstract: The objective of this study was the development of a statistical approach for radiolabeling optimization of cysteine-dextran conjugates with Tc-99m tricarbonyl core. This strategy has been applied to the labeling of 2-propylene-S-cysteine-dextran in the attempt to prepare a new class of tracers for sentinel lymph node detection, and can be extended to other radiopharmaceuticals for different targets. The statistical routine was based on three-level factorial design. Best labeling conditions were achieved. The specific activity reached was 5 MBq/μg.
authors.affiliation: Radiopharmacy Center, Institute of Energetic and Nuclear Research, Sao Paulo, SP, Brazil. eutimiocu@yahoo.com
authors.forename: Eutimio Gustavo Fernández
authors.initials: EG
authors.lastname: Núñez
authors.forename: Bluma Linkowski
authors.initials: BL
authors.lastname: Faintuch
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Teodoro
authors.forename: Danielle Pereira
authors.initials: DP
authors.lastname: Wiecek
authors.forename: Natanael Gomes
authors.initials: NG
authors.lastname: da Silva
authors.forename: Minas
authors.initials: M
authors.lastname: Papadopoulos
authors.forename: Maria
authors.initials: M
authors.lastname: Pelecanou
authors.forename: Ioannis
authors.initials: I
authors.lastname: Pirmettis
authors.forename: Renato Santos
authors.initials: RS
authors.lastname: de Oliveira Filho
authors.forename: Adriano
authors.initials: A
authors.lastname: Duatti
authors.forename: Roberto
authors.initials: R
authors.lastname: Pasqualini
mesh_terms: D002850:Chromatography, Gel; D003545:Cysteine / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D015609:Organotechnetium Compounds / Q000737:chemistry*; D011786:Quality Control
chemical_list: D003911:Dextrans; D015609:Organotechnetium Compounds; D003545:Cysteine