title: Human thymic stromal lymphopoietin enhances expression of CD80 in human CD14+ monocytes/macrophages.
abstract: OBJECTIVE
  Human thymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine, promotes inflammatory T helper type 2 cell (Th2) differentiation of naive CD4(+) T cells. TSLP is highly produced in keratinocytes of patients with atopic dermatitis and bronchial epithelia of patients with asthma and was thought to be a master switch for allergic inflammation. We sought to examine the effect of TSLP in human monocytes/macrophages.
  
  
  METHODS
  The effect of TSLP on the expression of cell surface antigens (CD11c, CD16, CD54, CD80, CD86, and HLA-DR) in peripheral blood CD14(+) monocytes/macrophages was examined.
  
  
  RESULTS
  TSLP enhanced the expression of CD80 in peripheral blood CD14(+) monocytes/macrophages but not that of other cell surface antigens. It was associated with an increased percentage of CD14(dim/-), CD80(+), CD11c(+), and HLA-DR(+) cells, which was consistent with the increased differentiation of myeloid dendritic cells.
  
  
  CONCLUSIONS
  TSLP induces CD80 expression in human peripheral blood CD14(+) monocytes/macrophages; this indicates monocyte/macrophage activation. This may be associated with their differentiation into myeloid dendritic cells.
authors.affiliation: Department of Pediatrics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-kogushi, Ube, Yamaguchi, 755-8505, Japan. rh@yamaguchi-u.ac.jp
authors.forename: Reiji
authors.initials: R
authors.lastname: Hirano
authors.forename: Shunji
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Kunio
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Haneda
authors.forename: Ayami
authors.initials: A
authors.lastname: Ohsaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichiyama
mesh_terms: D000328:Adult; D018122:B7-1 Antigen / Q000276:immunology*; D002454:Cell Differentiation; D016207:Cytokines / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D018122:B7-1 Antigen; D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D000094632:Thymic Stromal Lymphopoietin