title: Proliferative responses of Brugia malayi TPX-1 and its epitopic peptide(29-43) in an endemic population of human lymphatic filariasis.
abstract: Although the antioxidant thioredoxin peroxidase (TPX) is a putative target exploited in vaccine studies of lymphatic filariasis, the high sequence homology with host peroxiredoxins remains a great concern. The emergence of immunomics offers a powerful tool for novel vaccine design. Further, due to the cellular hypo-response in filariasis, analysis of T epitope repertoire becomes imperative in disease control. Here, we report the cellular responses of filarial TPX-1 and the identification of T epitope (29-43) in the host non-homologous region. The strong proliferative responses induced by the peptide mimetic in mice splenocytes and human PBMC's prove the existence of T epitope recognized in endemic population.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India.
authors.forename: Jayaprakasam
authors.initials: J
authors.lastname: Madhumathi
authors.forename: Gandhirajan
authors.initials: G
authors.lastname: Anugraha
authors.forename: Prabhu Rajaiah
authors.initials: PR
authors.lastname: Prince
authors.forename: Dhinakar
authors.initials: D
authors.lastname: Pradiba
authors.forename: Perumal
authors.initials: P
authors.lastname: Kaliraj
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D017178:Brugia malayi / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000276:immunology* / Q000469:parasitology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D054464:Peroxiredoxins / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D018984:Epitopes, T-Lymphocyte; D054464:Peroxiredoxins