title: Evaluation of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a screening method for the detection of influenza viruses in the fecal materials of water birds.
abstract: Migratory water birds are a natural reservoir for influenza A viruses. Viruses replicate in the intestines of ducks and are shed with the fecal materials. Virus isolation from collected fecal materials, therefore, is an integral part of the surveillance of avian influenza in water birds. In the present study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was assessed for its usefulness in detecting the RNA of influenza A viruses in fecal materials. It was found that, RT-LAMP specifically and sensitively detects the matrix gene of influenza A viruses. Influenza A viruses were isolated from the fecal materials in which viral RNA were detected by RT-LAMP in 35 min. The present findings indicate that RT-LAMP is useful as a high throughput screening method for field samples prior to virus isolation, allowing the processing of hundreds of samples per day.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Sapporo 060–0818, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Mayumi
authors.initials: M
authors.lastname: Endo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Motoshima
authors.forename: Fumi
authors.initials: F
authors.lastname: Yoshino
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okamatsu
authors.forename: Takahiro
authors.initials: T
authors.lastname: Soejima
authors.forename: Syouhei
authors.initials: S
authors.lastname: Senba
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kanda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000818:Animals; D004197:Disease Reservoirs / Q000662:veterinary; D004372:Ducks; D005243:Feces / Q000821:virology*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014763:Viral Matrix Proteins