title: Pseudomonas composti sp. nov., isolated from compost samples.
abstract: Two unusual, Gram-negative, catalase- and oxidase-positive rods, designated C2(T) and C5, were isolated from compost samples. Comparative 16S rRNA gene sequencing studies demonstrated that both isolates were members of the genus Pseudomonas and belonged to the Pseudomonas aeruginosa group. Strain C2(T) was most closely related to Pseudomonas cuatrocienegasensis 1N(T) and Pseudomonas borbori R-20821(T) (97.9 and 97.8% 16S rRNA gene sequence similarity, respectively). However, phylogenetic analysis based on rpoD gene sequences revealed that both isolates could be discriminated from members of the P. aeruginosa group that exhibited >97% 16S rRNA gene sequence similarity. The DNA G+C content of strain C2(T) was 61.5 mol%. The major fatty acids of strain C2(T) were a summed feature (C(16:1)ω7c and/or iso-C(15:0) 2-OH), C(18:1)ω7c/12t/9t, C(16:0) and C(12:0), which supported the isolates' affiliation with the genus Pseudomonas. Moreover, strain C2(T) could be distinguished from its closest phylogenetic neighbours of the genus Pseudomonas by DNA-DNA hybridization studies and biochemical tests. On the basis of both phenotypic and phylogenetic findings, it is proposed that the isolates be classified as a novel species, with the name Pseudomonas composti sp. nov. The type strain is C2(T) (=CECT 7516(T)=CCUG 59231(T)).
authors.affiliation: Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Gibello
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain.|Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Vela
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: Martín
authors.affiliation: Natural Biotec S.L., Parque Científico de Madrid, Ctra. de Colmenar Viejo, km 15. Cantoblanco, Pabellón C, 28049 Madrid, Spain.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Mengs
authors.affiliation: Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Pilar Z
authors.initials: PZ
authors.lastname: Alonso
authors.affiliation: Natural Biotec S.L., Parque Científico de Madrid, Ctra. de Colmenar Viejo, km 15. Cantoblanco, Pabellón C, 28049 Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Garbi
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain.|Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Jose F
authors.initials: JF
authors.lastname: Fernández-Garayzábal
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S