title: Visualization of superparamagnetic nanoparticles in vascular tissue using XμCT and histology.
abstract: In order to increase the dose of antineoplastic agents in the tumor area, the concept of magnetic drug targeting (MDT) has been developed. Magnetic nanoparticles consisting of iron oxide and a biocompatible cover layer suspended in an aqueous solution (ferrofluid) serve as carriers for chemotherapeutics being enriched by an external magnetic field after intra-arterial application in desired body compartments (i.e., tumor). We established an ex vivo model to simulate in vivo conditions in a circulating system consisting of magnetic iron oxide nanoparticles passing an intact bovine artery and being focused by an external magnetic field to study their distribution in the vessel. Micro-computed X-ray tomography (XμCT) and histology can elucidate the arrangement of these particles after application. XμCT-analysis has been performed on arterial sections after MDT in order to determine the distribution of the nanoparticles. These measurements have been carried out with a cone X-ray source and corresponding histological sections were stained with Prussian blue. It could be shown that combining XμCT and histology offers the opportunity for a better understanding of the mechanisms of nanoparticle deposition in the vascular system after MDT.
authors.affiliation: Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine, Else Kröner-Fresenius-Stiftung, Waldstr. 1, 91054 Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Tietze
authors.forename: Helene
authors.initials: H
authors.lastname: Rahn
authors.forename: Stefan
authors.initials: S
authors.lastname: Lyer
authors.forename: Eveline
authors.initials: E
authors.lastname: Schreiber
authors.forename: Jenny
authors.initials: J
authors.lastname: Mann
authors.forename: Stefan
authors.initials: S
authors.lastname: Odenbach
authors.forename: Christoph
authors.initials: C
authors.lastname: Alexiou
mesh_terms: D001158:Arteries / Q000033:anatomy & histology / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D005290:Ferric Compounds / Q000008:administration & dosage*; D005295:Ferrocyanides; D008280:Magnetics; D053758:Nanoparticles / Q000008:administration & dosage*; D055114:X-Ray Microtomography
chemical_list: D005290:Ferric Compounds; D005295:Ferrocyanides; C000499:ferric oxide; C000170:ferric ferrocyanide