title: Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells.
abstract: To investigate the effects of chronic exposure to arsenite on the gene expression profiles of mast cells, microarray analysis was performed on rat basophilic leukemia RBL-2H3 cells exposed to arsenite for 28 days. Upregulated genes include calcium-binding S100 proteins such as S100A9, S100A10, S100A6, and S100A13, and granzymes B and C. Among S100 proteins, S100A9 showed the highest expression (8.62-fold of untreated cells) after 4-weeks of exposure to arsenite. As S100A8 and S100A9 comprise a heterodimer called calprotectin, and are implicated in the development of atherosclerosis and cancer, mRNA levels of both S100A8 and S100A9 were analyzed. The results demonstrated that exposure of RBL-2H3 cells to arsenite for a few weeks induces marked increases in mRNA levels of S100A8 and S100A9.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Hitomi
authors.initials: H
authors.lastname: Fujishiro
authors.forename: Kanako
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Daigo
authors.initials: D
authors.lastname: Sumi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Satoh
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Himeno
mesh_terms: D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D050197:Atherosclerosis / Q000209:etiology; D040501:Calgranulin A / Q000235:genetics*; D040502:Calgranulin B / Q000235:genetics*; D020869:Gene Expression Profiling; D015471:Leukemia, Basophilic, Acute; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D046228:Microarray Analysis; D009369:Neoplasms / Q000209:etiology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017670:Sodium Compounds / Q000633:toxicity*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D018053:Arsenites; D040501:Calgranulin A; D040502:Calgranulin B; D012333:RNA, Messenger; D017670:Sodium Compounds; D014874:Water Pollutants, Chemical; C017947:sodium arsenite