title: Demethylation of oligogalacturonides by FaPE1 in the fruits of the wild strawberry Fragaria vesca triggers metabolic and transcriptional changes associated with defence and development of the fruit.
abstract: Ectopic expression of the strawberry (Fragaria×ananassa) gene FaPE1 encoding pectin methyl esterase produced in the wild species Fragaria vesca partially demethylated oligogalacturonides (OGAs), which conferred partial resistance of ripe fruits to the fungus Botrytis cinerea. Analyses of metabolic and transcriptional changes in the receptacle of the transgenic fruits revealed channelling of metabolites to aspartate and aromatic amino acids as well as phenolics, flavanones, and sesquiterpenoids, which was in parallel with the increased expression of some genes related to plant defence. The results illustrate the changes associated with resistance to B. cinerea in the transgenic F. vesca. These changes were accompanied by a significant decrease in the auxin content of the receptacle of the ripe fruits of transgenic F. vesca, and enhanced expression of some auxin-repressed genes. The role of these OGAs in fruit development was revealed by the larger size of the ripe fruits in transgenic F. vesca. When taken together these results show that in cultivated F. ananassa FaPE1 participates in the de-esterification of pectins and the generation of partially demethylated OGAs, which might reinforce the plant defence system and play an active role in fruit development.
authors.affiliation: Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, 29071 Málaga, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Osorio
authors.forename: Aureliano
authors.initials: A
authors.lastname: Bombarely
authors.forename: Patrick
authors.initials: P
authors.lastname: Giavalisco
authors.forename: Björn
authors.initials: B
authors.lastname: Usadel
authors.forename: Camilla
authors.initials: C
authors.lastname: Stephens
authors.forename: Irene
authors.initials: I
authors.lastname: Aragüez
authors.forename: Nieves
authors.initials: N
authors.lastname: Medina-Escobar
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Botella
authors.forename: Alisdair R
authors.initials: AR
authors.lastname: Fernie
authors.forename: Victoriano
authors.initials: V
authors.lastname: Valpuesta
mesh_terms: D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002473:Cell Wall / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D004951:Esterification; D031985:Fragaria / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism; D008745:Methylation; D008954:Models, Biological; D009844:Oligosaccharides / Q000378:metabolism*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000235:genetics
chemical_list: D007210:Indoleacetic Acids; D009844:Oligosaccharides; D012333:RNA, Messenger; C412763:oligogalacturonic acid; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase