title: Transcription factor Sp1 regulates basal transcription of the human DRG2 gene.
abstract: Developmentally regulated GTP-binding protein 2 (DRG2) is an evolutionarily conserved GTP-binding protein. DRG2 mRNA expression has been confirmed in many animal and human tissues. DRG2 is thought to play an essential role in the control of cell growth and differentiation. However, transcriptional regulation of DRG2 is largely unknown. To investigate the mechanisms controlling DRG2 expression, we cloned 1509bp of the 5'-flanking sequence of this gene. Deletion analysis showed that the region between -113 and -70 is essential for the basal level expression of the DRG2 gene in K562 human erythroleukemic cells. Mutation of a putative stimulating protein 1 (Sp1) regulatory site located at position -108 resulted in a significant decline in DRG2 promoter activity. Electrophoretic mobility shift assay and chromatin immunoprecipitation analysis revealed that Sp1 binds to this site. Knockdown of Sp1 expression using siRNA inhibited the promoter activation as well as the endogenous DRG2 transcriptional level. Taken together, these results demonstrate that basal expression level of DRG2 is regulated by the Sp1 transcription factor.
authors.affiliation: Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea.
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Myoung Seok
authors.initials: MS
authors.lastname: Ko
authors.forename: Hong Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Wha Ja
authors.initials: WJ
authors.lastname: Cho
authors.forename: Seon Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Byung Ju
authors.initials: BJ
authors.lastname: Lee
authors.forename: Jeong Woo
authors.initials: JW
authors.lastname: Park
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002470:Cell Survival; D004252:DNA Mutational Analysis; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006801:Humans; D020014:K562 Cells; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C409877:DRG2 protein, human; D016329:Sp1 Transcription Factor; D019204:GTP-Binding Proteins