title: A new immune-modulating diet enriched with whey-hydrolyzed peptide, fermented milk, and isomaltulose attenuates gut ischemia-reperfusion injury in mice.
abstract: OBJECTIVE
  Gut ischemia-reperfusion (I/R) is considered an important mechanism underlying multiple organ failure after severe surgical insults. We previously demonstrated an enteral diet enriched with whey-hydrolyzed peptide, fermented milk, and isomaltulose to have anti-inflammatory effects in a concanavalin A-induced hepatitis model. Here, we investigated whether the immune-modulating diet (IMD), could prevent systemic inflammation, thereby improving survival in a gut I/R model.
  
  
  METHODS
  Mice were randomized into control enteral diet (n = 58) or IMD (n = 56) for 2 weeks' feeding. In experiment 1, 39 mice underwent 45 min of gut ischemia, and were sacrificed at 3 h after reperfusion to collect blood samples. Plasma IL-6 and glucose levels were measured. In experiment 2, 75 mice underwent 60 min of ischemia, and their survival was observed until 48 h.
  
  
  RESULTS
  Plasma IL-6 and glucose levels of the IMD group were significantly lower than those of control mice. In association with these changes, the IMD improved survival rate at early time points (12 and 30 h) after gut I/R (p < 0.05, χ(2) test).
  
  
  CONCLUSIONS
  Nutritional management with the IMD may be useful for preventing systemic inflammatory response after gut I/R.
authors.affiliation: Department of Nutrition Research, Food Science Institute, Meiji Dairies Corporation, 540 Naruda, Odawara, Kanagawa 250-0862, Japan. KENTAROU_NAKAMURA@MEIJI-MILK.COM
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Saori
authors.initials: S
authors.lastname: Ogawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Dairiki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Fukatsu
authors.forename: Hajime
authors.initials: H
authors.lastname: Sasaki
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kaneko
authors.forename: Taketo
authors.initials: T
authors.lastname: Yamaji
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003611:Dairy Products / Q000032:analysis*; D004032:Diet; D004195:Disease Models, Animal; D004750:Enteral Nutrition / Q000379:methods; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000503:physiopathology; D006868:Hydrolysis; D007249:Inflammation / Q000517:prevention & control; D015850:Interleukin-6 / Q000097:blood; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008894:Milk Proteins / Q000008:administration & dosage*; D009102:Multiple Organ Failure / Q000517:prevention & control; D015427:Reperfusion Injury / Q000178:diet therapy*; D000067816:Whey Proteins
chemical_list: D001786:Blood Glucose; D015850:Interleukin-6; D008894:Milk Proteins; D000067816:Whey Proteins; D007534:Isomaltose; C008189:isomaltulose