title: Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery.
abstract: Local delivery of DNA through a hydrogel scaffold would increase the applicability of gene therapy in tissue regeneration and cancer therapy. However, the delivery of DNA/cationic polymer nanoparticles (polyplexes) using hydrogels is challenging due to the aggregation and inactivation of polyplexes during their incorporation into hydrogel scaffolds. We developed a novel process (termed caged nanoparticle encapsulation or CnE) to load concentrated and unaggregated non-viral gene delivery nanoparticles into various hydrogels. Previously, we showed that PEG hydrogels loaded with DNA/PEI polyplexes through this process were able to deliver genes both in vitro and in vivo. In this study, we found that hyaluronic acid and fibrin hydrogels with concentrated and unaggregated polyplexes loaded through CnE were able to deliver genes in vivo as well, demonstrating the universality of the process.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, BH5531, Los Angeles, CA 90095, USA.
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Lei
authors.forename: Maha
authors.initials: M
authors.lastname: Rahim
authors.forename: Quinn
authors.initials: Q
authors.lastname: Ng
authors.forename: Tatiana
authors.initials: T
authors.lastname: Segura
mesh_terms: D000818:Animals; D001681:Biological Assay; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000378:metabolism; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D005337:Fibrin / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007097:Imines / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D018919:Neovascularization, Physiologic; D010316:Particle Size; D011095:Polyethylenes / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D020100:Hydrogels; D007097:Imines; D011095:Polyethylenes; D042461:Vascular Endothelial Growth Factor A; C505405:poly(ethylene imine); D005337:Fibrin; D006820:Hyaluronic Acid; D004247:DNA; D001616:beta-Galactosidase