title: The protective effects of nutritional antioxidant therapy on Ehrlich solid tumor-bearing mice depend on the type of antioxidant therapy chosen: histology, genotoxicity and hematology evaluations.
abstract: Strong evidence indicates that reactive oxygen species (ROS) play an important role in the initiation as well as the promotion phase of carcinogenesis. Studies support the role of ROS in cancer, in part, by showing that dietary antioxidants act as cancer-preventive agents. Although results are promising, the research on this topic is still controversial. Thus, the aim of this study was to investigate whether vitamins C, E and pequi oil can, individually, provide prevention and/or be used afterward as an adjuvant in cancer therapy. Ehrlich solid tumor-bearing mice received antioxidant as follows: before tumor inoculation, before and after tumor inoculation (continuous administration), and after tumor inoculation; morphometric analyses of tumor, genotoxicity and hematology were then carried out. Antioxidant administrations before tumor inoculation effectively inhibited its growth in the three experimental protocols, but administrations after the tumor's appearance accelerated tumor growth and favored metastases. Continuous administration of pequi oil inhibited the tumor's growth, while the same protocol with vitamins E and C accelerated it, favoring metastasis and increasing oxidative stress on erythrocytes. Except for continuous administration with vitamin E, the development of ascites tumor metastases was linked with increased inflammation. Results suggest that the efficiency and applicability of antioxidants in the medical clinic can depend not only on the nature of the antioxidant, the type and stage of cancer being treated and the prevailing oxygen partial pressure in the tissues, but also on the type of antioxidant therapy chosen.
authors.affiliation: Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília/DF, Brasil. mirandavilela@unb.br
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Miranda-Vilela
authors.forename: Flávia A
authors.initials: FA
authors.lastname: Portilho
authors.forename: Vitor G B
authors.initials: VG
authors.lastname: de Araujo
authors.forename: Luciana L C
authors.initials: LL
authors.lastname: Estevanato
authors.forename: Bélin P
authors.initials: BP
authors.lastname: Mezzomo
authors.forename: Maria de Fátima M de Almeida
authors.initials: Mde F
authors.lastname: Santos
authors.forename: Zulmira G M
authors.initials: ZG
authors.lastname: Lacava
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000627:therapeutic use*; D001772:Blood Cell Count; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology / Q000556:secondary; D002338:Carotenoids / Q000627:therapeutic use*; D020552:Comet Assay; D020448:Ericales / Q000737:chemistry; D005260:Female; D051379:Mice; D009369:Neoplasms / Q000517:prevention & control; D010938:Plant Oils / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D010938:Plant Oils; D017382:Reactive Oxygen Species; D014810:Vitamin E; D002338:Carotenoids; D001205:Ascorbic Acid