title: Identification and characterization of the Streptomyces globisporus 1912 regulatory gene lndYR that affects sporulation and antibiotic production.
abstract: Here, we report the identification and functional characterization of the Streptomyces globisporus 1912 gene lndYR, which encodes a GntR-like regulator of the YtrA subfamily. Disruption of lndYR arrested sporulation and antibiotic production in S. globisporus. The results of in vivo and in vitro studies revealed that the ABC transporter genes lndW-lndW2 are targets of LndYR repressive action. In Streptomyces coelicolor M145, lndYR overexpression caused a significant increase in the amount of extracellular actinorhodin. We suggest that lndYR controls the transcription of transport system genes in response to an as-yet-unidentified signal. Features that distinguish lndYR-based regulation from other known regulators are discussed.
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Bohdan
authors.initials: B
authors.lastname: Ostash
authors.affiliation: Department of Microbiology, Niigata University of Pharmacy and Applied Life Sciences, Higashijima 265-1, 956-8603 Niigata, Japan.|Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA.|Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Rebets
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Maksym
authors.initials: M
authors.lastname: Myronovskyy
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Olga
authors.initials: O
authors.lastname: Tsypik
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Iryna
authors.initials: I
authors.lastname: Ostash
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Kulachkovskyy
authors.affiliation: Department of Physics of the Earth, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Datsyuk
authors.affiliation: Department of Microbiology, Niigata University of Pharmacy and Applied Life Sciences, Higashijima 265-1, 956-8603 Niigata, Japan.
authors.forename: Tatsunosuke
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Walker
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo St 4, L'viv 79005, Ukraine.
authors.forename: Victor
authors.initials: V
authors.lastname: Fedorenko
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000235:genetics / Q000254:growth & development*; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012097:Repressor Proteins