title: Usefulness of published PCR primers in detecting human rhinovirus infection.
abstract: We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
authors.affiliation: The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Cassandra E
authors.initials: CE
authors.lastname: Faux
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Arden
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Lambert
authors.forename: Michael D
authors.initials: MD
authors.lastname: Nissen
authors.forename: Terry M
authors.initials: TM
authors.lastname: Nolan
authors.forename: Anne B
authors.initials: AB
authors.lastname: Chang
authors.forename: Theo P
authors.initials: TP
authors.lastname: Sloots
authors.forename: Ian M
authors.initials: IM
authors.lastname: Mackay
mesh_terms: D017931:DNA Primers / Q000235:genetics; D006801:Humans; D010850:Picornaviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D012229:Rhinovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D012367:RNA, Viral