title: In vitro study of the effects of plaunotol on oral cell proliferation and wound healing.
abstract: Plaunotol is an acyclic diterpene alcohol extracted from a medicinal plant called plau-noi, Croton stellatopilosus Ohba, and has been widely used for the treatment of gastric ulcers in Japan. The aim of this study was to examine the effects of plaunotol on human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs). To assess the cytotoxic effect, HGFs and HOKs were treated with plaunotol. Subsequently, the morphology of cells was recorded and cells were subjected to MTT assay. To investigate cell proliferation effect, cells were treated with plaunotol and counted with a haemocytometer. To determine wound healing effect, the number of cells repopulated into the wounded areas in monolayer culture and in fibroblast-populated collagen lattice (FPCL) was measured. The results showed that 10 and 1 μg/ml (33 and 3.3 μmol/l) plaunotol induced toxicity in HGFs and HOKs, respectively. However, 0.1 μg/ml (0.33 μmol/l) plaunotol promoted HGF proliferation and wound healing in monolayer and FPCL models. In contrast, 0.1 μg/ml plaunotol could not induce HOK proliferation nor in vitro wound healing using monolayer culture, but it induced wound healing in a modified FPCL model. Our data suggested that plaunotol could promote oral cell proliferation and wound healing in vitro and may have an implication on oral wound healing.
authors.affiliation: Department of Hospital Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
authors.forename: Siribang-on Piboonniyom
authors.initials: SO
authors.lastname: Khovidhunkit
authors.forename: Nadiya
authors.initials: N
authors.lastname: Yingsaman
authors.forename: Kongtawat
authors.initials: K
authors.lastname: Chairachvit
authors.forename: Rudee
authors.initials: R
authors.lastname: Surarit
authors.forename: Pornpoj
authors.initials: P
authors.lastname: Fuangtharnthip
authors.forename: Amorn
authors.initials: A
authors.lastname: Petsom
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D029802:Croton / Q000737:chemistry; D004224:Diterpenes; D005233:Fatty Alcohols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D010946:Plants, Medicinal / Q000737:chemistry; D013276:Stomach Ulcer / Q000188:drug therapy; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004224:Diterpenes; D005233:Fatty Alcohols; C047686:plaunotol