title: A new azafluorenone from the roots of Polyalthia cerasoides and its biological activity.
abstract: Chromatographic separation of the ethyl acetate extract of roots of Polyalthia cerasoides has led to the isolation of the new compound, 6,8-dihydroxy-7-methoxy-1-methyl-azafluorenone. This compound exhibited potent cytotoxic activities with IC50 values in the range of 2.64-3.58 microg x mL(-1) for A549, GLC4 and GLC4/Adr cells, but was not recognized by ABCC1/MRP1 protein. The compound also showed very strong inhibition of M. tuberculosis using a broth microdilution method, with an MIC value of 0.78 microg x mL(-1), which was equal to that of ofloxacin, one of the four antibiotic drugs used as a positive control.
authors.affiliation: Laboratory of Natural Products, Faculty of Science and Center for Innovation in Chemistry, Lampang Rajabhat University, 52100 Lampang, Thailand.
authors.forename: Kanchana
authors.initials: K
authors.lastname: Pumsalid
authors.forename: Haruthai
authors.initials: H
authors.lastname: Thaisuchat
authors.forename: Chatchanok
authors.initials: C
authors.lastname: Loetchutinat
authors.forename: Narong
authors.initials: N
authors.lastname: Nuntasaen
authors.forename: Puttinan
authors.initials: P
authors.lastname: Meepowpan
authors.forename: Wilart
authors.initials: W
authors.lastname: Pompimon
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D005449:Fluorenes / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D031044:Polyalthia / Q000737:chemistry*
chemical_list: C556325:6,8-dihydroxy-7-methoxy-1-methyl-azafluorenone; D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D005449:Fluorenes