title: Five challenges to bringing single-molecule force spectroscopy into living cells.
abstract: In recent years, single-molecule force spectroscopy techniques have been used to study how inter- and intramolecular interactions control the assembly and functional state of biomolecular machinery in vitro. Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo.
authors.affiliation: Universite catholique de Louvain, Institute of Condensed Matter and Nanosciences, Louvain-la-Neuve, Belgium.
authors.forename: Yves F
authors.initials: YF
authors.lastname: Dufrêne
authors.forename: Evan
authors.initials: E
authors.lastname: Evans
authors.forename: Andreas
authors.initials: A
authors.lastname: Engel
authors.forename: Jonne
authors.initials: J
authors.lastname: Helenius
authors.forename: Hermann E
authors.initials: HE
authors.lastname: Gaub
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Müller
mesh_terms: D000818:Animals; D002470:Cell Survival; D006801:Humans; D018625:Microscopy, Atomic Force; D013057:Spectrum Analysis / Q000379:methods*