title: An indirect competitive enzyme-linked immunosorbent assay for determination of norfloxacin in waters using a specific polyclonal antibody.
abstract: A specific polyclonal anti-norfloxacin antibody was obtained, and a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for determining trace amounts of norfloxacin in various waters. Good linearity was achieved in the range from 0.1 to 10 μg L(-1). The average IC(50) value was determined to be 2.2 μg L(-1) and the limit of detection was 0.016 μg L(-1) at a signal-to-noise ratio of 3 in phosphate-buffered saline buffer. Recoveries of norfloxacin at various spiking levels ranged from 74 to 105% in groundwater, surface water, treated and untreated wastewater samples, with relative standard deviations of 3-5%. The assay was applied for determining norfloxacin in municipal wastewater, surface water, and groundwater collected in a metropolis of China. Raw wastewater samples were only submitted to filtration and pH adjustment while the other water samples were pre-concentrated by solid phase extraction prior to the icELISA assay. Good agreement of the results obtained by the icELISA and liquid chromatography tandem mass spectrometry further confirmed the reliability and accuracy of the icELISA for rapid detection of norfloxacin in waters.
authors.affiliation: State key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 511 Kehua St, 510640 Guangzhou, Guangdong, China.
authors.forename: Jianlan
authors.initials: J
authors.lastname: Cui
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Qiuxin
authors.initials: Q
authors.lastname: Huang
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Yu
authors.forename: Xianzhi
authors.initials: X
authors.lastname: Peng
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D009643:Norfloxacin / Q000032:analysis*; D011817:Rabbits; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000906:Antibodies; D014874:Water Pollutants, Chemical; D014867:Water; D009643:Norfloxacin