title: Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin.
abstract: In this investigation we report the synthesis of optically coupled hybrid architectures based on a new biomimetic fluorescent protein rec1-resilin and nanometer-scale gold nanoparticles (AuNPs) in a one-step method using a non-covalent mode of binding protocol. The presence of uniformly distributed fluorophore sequences, -Ser(Thr)-Tyr-Gly- along the molecular structure of rec1-resilin provides significant opportunity to synthesize fluorophore-modified AuNPs bioconjugates with unique photophysical properties. The detailed analyses of the AuNP-bioconjugates, synthesized under different experimental conditions using spectroscopic, microscopic and scattering techniques demonstrate the organizational pathways and the electronic and photophysical properties of the developed AuNP-rec1-resilin bioconjugates. The calculation of the bimolecular quenching constant using the Stern-Volmer equation confirms that the dominant mechanism involved in quenching of fluorescence of rec1-resilin in the presence of AuNP is static. Photoacoustic infrared spectroscopy was employed to understand the nature of the interfacial interaction between the AuNP and rec1-resilin and its evolution with pH. In such bioconjugates the quenched emission of fluorescence by AuNP on the fluorophore moiety of rec1-resilin in the immediate vicinity of the AuNP has significant potential for fluorescence-based detection schemes, sensors and also can be incorporated into nanoparticle-based devices.
authors.affiliation: Ian Wark Research Institute, ARC Special Research Centre, Mawson Lakes Campus, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Sundar
authors.initials: S
authors.lastname: Mayavan
authors.forename: Naba K
authors.initials: NK
authors.lastname: Dutta
authors.forename: Namita R
authors.initials: NR
authors.lastname: Choudhury
authors.forename: Misook
authors.initials: M
authors.lastname: Kim
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Elvin
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Hill
mesh_terms: D032701:Biomimetics; D006046:Gold / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D020349:Surface Plasmon Resonance
chemical_list: D019476:Insect Proteins; C030951:resilin; D006046:Gold