title: [Identification of a novel allele HLA-DRB1*1219].
abstract: OBJECTIVE
  To identify a novel HLA DRB1 allele in a Chinese leukemia family.
  
  
  METHODS
  A new HLA-DRB1 allele was initially detected by polymerase chain reaction-sequence specific primer and unusual reaction pattern by Luminex RSSO, then DNA sequencing was performed to identify the sequence of the novel allele.
  
  
  RESULTS
  The DNA sequencing revealed the presence of the new allele which differs from the closest matching HLA-DRB1*120201 by a single nucleotide substitution at position (341 C > T in exon 2), resulting in an amino acid change from Ala to Val at coden 85.
  
  
  CONCLUSIONS
  A novel allele was confirmed by DNA sequencing and has been designated HLA-DRB1*1219 by the WHO Nomenclature Committee.
authors.affiliation: China Marrow Donor Program HLA Typing Laboratory, Shandong Blood Center, Jinan, Shandong, 250014 PR China.
authors.forename: Xiang-min
authors.initials: XM
authors.lastname: Nie
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yun-hai
authors.initials: YH
authors.lastname: Fang
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Song
authors.forename: Yun-long
authors.initials: YL
authors.lastname: Zhuang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Chuan-fu
authors.initials: CF
authors.lastname: Zhu
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains