title: Quality of frozen-thawed semen in brown bear is not affected by timing of glycerol addition.
abstract: We have tested several freezing protocols for brown bear semen, modifying the time when glycerol was added (before and after cooling to 5 °C). No differences were found among protocols, indicating a good tolerance of brown bear semen to glycerol. This finding indicates that freezing protocols for brown bear semen could be modified to fit practical solutions which would facilitate preparation of the seminal samples in the field with the addition of glycerol at ambient temperature.
authors.affiliation: University of León, 24071 León, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Alvarez-Rodríguez
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: S
authors.initials: S
authors.lastname: Gomes-Alves
authors.forename: S
authors.initials: S
authors.lastname: Borragan
authors.forename: F
authors.initials: F
authors.lastname: Martinez-Pastor
authors.forename: P
authors.initials: P
authors.lastname: de Paz
authors.forename: L
authors.initials: L
authors.lastname: Anel
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013997:Time Factors; D001503:Ursidae / Q000502:physiology*
chemical_list: D005990:Glycerol