title: Development and validation of stable-isotope dilution liquid chromatography-tandem mass spectrometric method for determination of salivary progesterone.
abstract: A method for the quantification of progesterone (PROG) in human saliva using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) has been developed and validated. The saliva was deproteinized with acetonitrile, purified using a Strata™-X cartridge, and subjected to LC-ESI-MS/MS. Quantification was based on selected reaction monitoring, and deuterated PROG was used as the internal standard. This method allowed the reproducible (intra- and inter-assay relative standard deviations, <2.2%) and accurate (analytical recovery, 96.6-99.7%) quantification of the salivary PROG using a 400 μL sample, and the limit of quantification was 12.5 pg/mL. The developed method enabled detection of the variation in the salivary PROG concentrations of healthy volunteers during the menstrual cycle and measurement of the salivary concentrations of pregnant women. The method is expected to be an alternative to the blood PROG monitoring in clinical examinations, because saliva collection is easy, non-invasive and repeatable.
authors.affiliation: School of Pharmaceutical Sciences and Global COE Program, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan. higashi@u-shizuoka-ken.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Higashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.forename: Misato
authors.initials: M
authors.lastname: Narushima
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sugiura
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Inagaki
authors.forename: Jun Zhe
authors.initials: JZ
authors.lastname: Min
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Toyo'oka
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D011374:Progesterone