title: In vitro assessment of praziquantel and a novel nanomaterial against protoscoleces of Echinococcus granulosus.
abstract: The present study describes the activity of a nanomaterial on protoscoleces of Echinococcus granulosus, which exhibited morphological changes and apoptosis. Apoptotic changes were deduced on the basis of effector caspase activation and nucleosomal laddering. Invaginated protoscoleces maintained in vitro became evaginated and had hooks, presumptive suckers and stalks. Degenerative changes of protoscoleces were evidenced after treatment with praziquantel and nano-combination. Protoscoleces treated with praziquantel had distinct attestation of necrosis and nano-combination-treated protoscoleces had signatures of apoptosis.
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, West Bengal, India.
authors.forename: S
authors.initials: S
authors.lastname: De
authors.forename: D
authors.initials: D
authors.lastname: Pan
authors.forename: A K
authors.initials: AK
authors.lastname: Bera
authors.forename: V
authors.initials: V
authors.lastname: Sreevatsava
authors.forename: S
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: D
authors.initials: D
authors.lastname: Chaudhuri
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: T
authors.initials: T
authors.lastname: Rana
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: S K
authors.initials: SK
authors.lastname: Das
authors.forename: V V
authors.initials: VV
authors.lastname: Suryanaryana
authors.forename: M Norjit
authors.initials: MN
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Bhattacharya
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D048209:Echinococcus granulosus / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D015801:Helminth Proteins / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D011223:Praziquantel / Q000494:pharmacology*
chemical_list: D000871:Anthelmintics; D015801:Helminth Proteins; D011223:Praziquantel; D053148:Caspase 3