title: Global gene expression profiling in larval zebrafish exposed to microcystin-LR and microcystis reveals endocrine disrupting effects of Cyanobacteria.
abstract: Microcystis blooms occur worldwide and threaten aquatic ecosystems and human health. Sublethal effects on early developmental stages of fish are largely unknown, and research has mainly focused on microcystin toxins (such as MC-LR) rather than Microcystis cells. We exposed (96 h) zebrafish larvae to purified MC-LR (0-1000 μg/L) or lyophilized Microcystis aeruginosa containing 4.5 μg/L MC-LR and evaluated changes in global gene expression (Affymetrix GeneChip zebrafish genome arrays). Significant changes in gene expression (≥ 1.7-fold change, p < 0.0001) were determined with Rosetta Resolver 7.0, and ontology analysis was conducted with the DAVID bioinformatics tool. The number of differentially expressed genes relative to control increased with MC-LR concentration and included genes related to known mechanisms of action for MC-LR in mammals and older life stages of fish, as well as genes unique to larval zebrafish. Up-regulation of vitellogenin genes (vtg) (19.2-fold to >100-fold on arrays; 619.3-fold confirmed by quantitative PCR) was observed in Microcystis-exposed larvae but not in larvae exposed to MC-LR. Up-regulation of vtg indicates exposure to estrogenic substance(s) and suggests that Microcystis may be a natural source of environmental estrogens. Concerns about effects of Microcystis blooms may extend beyond those associated with the microcystin toxin.
authors.affiliation: Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37996, United States.
authors.forename: Emily D
authors.initials: ED
authors.lastname: Rogers
authors.forename: Theodore B
authors.initials: TB
authors.lastname: Henry
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Twiner
authors.forename: Julia S
authors.initials: JS
authors.lastname: Gouffon
authors.forename: Jackson T
authors.initials: JT
authors.lastname: McPherson
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Boyer
authors.forename: Gary S
authors.initials: GS
authors.lastname: Sayler
authors.forename: Steven W
authors.initials: SW
authors.lastname: Wilhelm
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D008387:Marine Toxins; D052998:Microcystins / Q000378:metabolism / Q000633:toxicity*; D046931:Microcystis / Q000254:growth & development / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; D029961:Zebrafish Proteins; C057862:cyanoginosin LR