title: The promotion of siRNA delivery to breast cancer overexpressing epidermal growth factor receptor through anti-EGFR antibody conjugation by immunoliposomes.
abstract: The LPD (liposome-polycation-DNA complex) is an effective nanovector for systemically small interfering RNA (siRNA) delivery which was well characterized previously. However, little effort was spend on the development of targeted LPD conjugated with tumor specific antibody (TLPD) which would be potent in promoting siRNA delivery in tumor. Here, we prepared TLPD through a self-assembling process followed by anti-EGFR antibody conjugation. The effect of antibody type, conjugation strategy and amount on the physicochemical and biological properties of TLPD was investigated. We obtained optimized TLPD conjugated with anti-EGFR Fab' by conventional conjugation (TLPD-FCC), which possessed a small size around 150nm and superior in vitro stability. Compared with nontargeted LPD (NTLPD), TLPD-FCC showed significantly enhanced binding affinity and luciferase gene silencing activity in EGFR overexpressing MDA-MB-231 breast cancer cells in vitro. Moreover, the in vivo accumulation of TLPD-FCC was obviously higher than that of NTLPD in MDA-MB-231 tumor 24h post intravenous injection. The promoted uptake of TLPD-FCC in MDA-MB-231 tumor was further confirmed by confocal microscopy. Notably, three intravenous injections of siRNA in TLPD-FCC significantly silenced luciferase expression by ∼20%, whereas NTLPD showed little effect. All these results suggested that our TLPD-FCC have a great potential in delivering siRNA to EGFR overexpressing breast cancers.
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, Shanghai, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Huaiwen
authors.initials: H
authors.lastname: Chen
authors.forename: Bohua
authors.initials: B
authors.lastname: Li
authors.forename: Dapeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Weizhu
authors.initials: W
authors.lastname: Qian
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Meng
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Jianming
authors.initials: J
authors.lastname: Chen
authors.forename: Yajun
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000502:physiology; D001943:Breast Neoplasms / Q000628:therapy*; D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D000906:Antibodies; D008081:Liposomes; D034741:RNA, Small Interfering; D066246:ErbB Receptors