title: Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing.
abstract: Our previous study has revealed that silver nanoparticles (AgNPs) have potential to promote wound healing by accelerated re-epithelization and enhanced differentiation of fibroblasts. However, the effect of AgNPs on the functionality of repaired skin is unknown. The aim of this study was to explore the tensile properties of healed skin after treatment with AgNPs. Immunohistochemical staining, quantitative assay and scanning electron microscopy (SEM) were used to detect and compare collagen deposition, and the morphology and distribution of collagen fibers. Our results showed that AgNPs improved tensile properties and led to better fibril alignments in repaired skin, with a close resemblance to normal skin. Based on our findings, we concluded that AgNPs were predominantly responsible for regulating deposition of collagen and their use resulted in excellent alignment in the wound healing process. The exact signaling pathway by which AgNPs affect collagen regeneration is yet to be investigated.
  
  
  BACKGROUND
  The aim of this study was to explore the tensile properties of healed skin after treatment with AgNPs. These nanoparticles improved tensile properties and led to better fibril alignments in repaired skin, with a close resemblance to normal skin. The exact signaling pathway by which AgNPs affect collagen regeneration is yet to be investigated.
authors.affiliation: Department of Orthopaedics and Traumatology, LKS Faculty of Medicine, University of Hong Kong, Hong Kong, China.
authors.forename: Karen H L
authors.initials: KH
authors.lastname: Kwan
authors.forename: Xuelai
authors.initials: X
authors.lastname: Liu
authors.forename: Michael K T
authors.initials: MK
authors.lastname: To
authors.forename: Kelvin W K
authors.initials: KW
authors.lastname: Yeung
authors.forename: Chi-ming
authors.initials: CM
authors.lastname: Ho
authors.forename: Kenneth K Y
authors.initials: KK
authors.lastname: Wong
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008855:Microscopy, Electron, Scanning; D012834:Silver / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D012834:Silver; D003094:Collagen