title: Determination of spinetoram in leafy vegetable crops using liquid chromatography and confirmation via tandem mass spectrometry.
abstract: Spinetoram is a second-generation member of the spinosyn class, all members of which have been shown to be effective in insect control via a novel mode of action. Spinetoram is a mixture of 3'-O-ethyl-5, 6-dihydro spinosyn J (XDE-175-J) and 3'-O-ethyl spinosyn L (XDE-175-L). In order to establish a determination method for the analysis of spinetoram residues in crops, commercial product (5% suspension concentrate spinetoram) was applied to two leafy vegetables (Garland chrysanthemum and Aster scaber) on different spraying schedules. The analytical method used herein was based on a reversed-phase separation on a C(18) column, isocratic elution and UV detection. The analytes were confirmed via tandem mass spectrometry. The method was linear over a concentration range of 0.05-10 ppm with a correlation coefficient in excess of 0.9998. The recoveries of XDE-175-J and XDE-175-L from the two vegetables ranged between 86.04 and 98.87% at spiking levels of 1 and 5 ppm. The relative standard deviations were no more than 7% for all recovery tests conducted herein. The calculated limits of detection and quantification were 0.01 and 0.03 ppm for both XDE-175-J and XDE-175-L. The levels of residues in two vegetables treated under a fixed schedule in the greenhouse were 6.21-0.55 ppm (maximum residue limit (MRL) = 7 ppm). In sum, this method constitutes an easy and reliable technique for the determination of spinetoram in leafy vegetables.
authors.affiliation: Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, Buk-Ku, Gwangju, Republic of Korea.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Jong-Hyouk
authors.initials: JH
authors.lastname: Park
authors.forename: Soon-Kil
authors.initials: SK
authors.lastname: Cho
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
mesh_terms: D031173:Aster Plant / Q000737:chemistry; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D031202:Chrysanthemum / Q000737:chemistry; D016018:Least-Squares Analysis; D018942:Macrolides / Q000032:analysis* / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D018942:Macrolides; D010573:Pesticide Residues; C548262:spinetoram