title: [Molecular mechanism of interleukin-13-induced mucus hypersecretion in rat airway].
abstract: OBJECTIVE
  To investigate the effect of interleukin-13 (IL-13) on mucus secretion in vivo and the possible mechanism.
  
  
  METHODS
  The SD rats were randomly divided into control group, IL-13 group and IL-13 plus SP600125 group. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase 1/2 (JNK1/2) and the level of MUC5AC in the lung tissues were examined using Western blotting. RT-PCR was performed to examine the mRNA level of STAT4 and STAT6, and electrophoretic mobility shift assays (EMSA) was used to detect the DNA-binding activities of Forkhead box a2 (FOXA2) and activator protein-1 (AP-1).
  
  
  RESULTS
  IL-13 caused a significant increase in MUC5AC and p-JNK1/2 expression, but did not affect the phosphorylation of ERK1/2. The expression of MUC5AC was attenuated after treatment with SP600125. A significant increase in STAT6 was observed in IL-13 group compared with that in the control group, whereas the expression of STAT4 mRNA was not significantly affected. The DNA-binding activity of FOXA2 was down-regulated after IL-13 exposure, which did not affect the DNA-binding activity of AP-1.
  
  
  CONCLUSIONS
  IL-13 down-regulates mucus secretion via STAT6-FOXA2 pathway in vitro.
authors.affiliation: Department of Respiratory Medicine, Chongqing Medical University, Chongqing 400010, China. gdp116@163.com
authors.forename: De-peng
authors.initials: DP
authors.lastname: Jiang
authors.forename: Victor P
authors.initials: VP
authors.lastname: Kolosov
authors.forename: Juliy M
authors.initials: JM
authors.lastname: Perelman
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D005260:Female; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics / Q000378:metabolism; D018793:Interleukin-13 / Q000494:pharmacology*; D008297:Male; D055271:Mucin 5AC / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D050800:STAT6 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C496504:Foxa2 protein, rat; D018793:Interleukin-13; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D012333:RNA, Messenger; D050800:STAT6 Transcription Factor; D051542:Hepatocyte Nuclear Factor 3-beta