title: Intact mammalian cell function on semiconductor nanowire arrays: new perspectives for cell-based biosensing.
abstract: Nanowires (NWs) are attracting more and more interest due to their potential cellular applications, such as delivery of compounds or sensing platforms. Arrays of vertical indium-arsenide (InAs) NWs are interfaced with human embryonic kidney cells and rat embryonic dorsal root ganglion neurons. A selection of critical cell functions and pathways are shown not to be impaired, including cell adhesion, membrane integrity, intracellular enzyme activity, DNA uptake, cytosolic and membrane protein expression, and the neuronal maturation pathway. The results demonstrate the low invasiveness of InAs NW arrays, which, combined with the unique physical properties of InAs, open up their potential for cellular investigations.
authors.affiliation: Bionanotechnology and Nanomedicine Laboratory, Department of Neuroscience and Pharmacology & Nano-science Center, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen, Denmark.
authors.forename: Trine
authors.initials: T
authors.lastname: Berthing
authors.forename: Sara
authors.initials: S
authors.lastname: Bonde
authors.forename: Claus B
authors.initials: CB
authors.lastname: Sørensen
authors.forename: Pawel
authors.initials: P
authors.lastname: Utko
authors.forename: Jesper
authors.initials: J
authors.lastname: Nygård
authors.forename: Karen L
authors.initials: KL
authors.lastname: Martinez
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000737:chemistry*; D015374:Biosensing Techniques; D005727:Ganglia, Spinal / Q000378:metabolism; D006801:Humans; D007204:Indium / Q000737:chemistry*; D018613:Microscopy, Confocal; D053770:Nanowires / Q000737:chemistry*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D012666:Semiconductors; D013329:Structure-Activity Relationship
chemical_list: D001152:Arsenicals; D007204:Indium; C076773:indium arsenide