title: DNA microarray analysis of human coronary artery endothelial cells exposed to cadmium.
abstract: Cadmium (Cd) is a toxic heavy metal that has been shown to induce vascular diseases, such as atherosclerosis. We used DNA microarray to monitor the transcriptional response of human coronary artery endothelial cells (HCAEC) to a non-lethal dose of Cd (10 µM). Out of 35,035 human genes, Cd enhanced the expression of 3 metallothionein (MT)-I subisoform genes, including MT1E, MT1H and MT1B, and reduced the expression of 12 genes, including ISG20 and TK1, 2-fold or greater.
authors.affiliation: Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University, Chikusa-ku, Nagoya, Aichi, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Akiko
authors.initials: A
authors.lastname: Honda
authors.forename: Chika
authors.initials: C
authors.lastname: Yamamoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kaji
authors.forename: Masahiko
authors.initials: M
authors.lastname: Satoh
mesh_terms: D050197:Atherosclerosis / Q000209:etiology; D019256:Cadmium Chloride / Q000633:toxicity*; D002478:Cells, Cultured; D015536:Down-Regulation; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005092:Exonucleases / Q000235:genetics*; D005095:Exoribonucleases; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008668:Metallothionein / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000032:analysis*; D013937:Thymidine Kinase / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D012313:RNA; D008668:Metallothionein; D013937:Thymidine Kinase; C092717:thymidine kinase 1; D005092:Exonucleases; D005095:Exoribonucleases; C112346:ISG20 protein, human; D019256:Cadmium Chloride