title: [Iodoacetamide-induced aquaporin 1 expression in fibroblasts is energy-dependent].
abstract: OBJECTIVE
  To investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1).
  
  
  METHODS
  Balb/c mouse fibroblasts were incubated with iodoacetamide (IA) in vitro, and the changes in AQP1 expression were detected by immunoblotting and immunohistochemistry at 0, 4, and 6 h.
  
  
  RESULTS
  IA induced the expression of AQP1 at 4 and 6 h accompanied with cell death. Reverse transcription PCR showed an increased expression of AQP1 mRNA in the cells. AQP1 expression was also upregulated by the inhibitor of microtubule and cytochrome C oxidase.
  
  
  CONCLUSIONS
  A pretranslational regulation occurs in IA-induced AQP1 expression in mouse fibroblasts, and the up-regulated AQP1 accumulation is characterized by mitochondria-related energy dependence.
authors.affiliation: Institute of Respiratory Diseases, First Affiliated Hospital, China Medical University, Shenyang 110001, China. lierran@hotmail.com
authors.forename: Er-ran
authors.initials: ER
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Hong
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Shang
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Qiu-yue
authors.initials: QY
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004734:Energy Metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D007460:Iodoacetamide / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C496021:Aqp1 protein, mouse; D012333:RNA, Messenger; D051398:Aquaporin 1; D007460:Iodoacetamide