title: [Variation of the V3 loop tip motifs and primary drug resistance of HIV-1 strains].
abstract: OBJECTIVE
  To study the characteristics of the variation of the V3 loop tip motifs and the drug resistance in the primary treatment patients.
  
  
  METHODS
  The partial region of the HIV-1 env and pol gene in 51 samples was amplified by nested polymerase chain reaction (PCR) ,purified products were cloned into the vectors, the obtained were analyzed by MEGA soft wares.
  
  
  RESULTS
  The V3 loop tip motifs had four types in our study (GPGR, GPGQ, GPGK, GQGR); the study on the drug resistance in primary treatment patients, showed that there were not major resistance associated with PI, and the resistance were minor mutations in protease gene. In the RT region, there were nine resistance mutants were single NRTIs or NNRTIs.
  
  
  CONCLUSIONS
  The GPGR which was the typical western V3 loop tip motifs attained to 44.44%. This results showed that the percentage of primary drug resistance was still low in our study region, suggesting no need for genotyping detection in blood donor patients before primary therapy.
authors.affiliation: State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Guo-min
authors.initials: GM
authors.lastname: Chen
authors.forename: Ze-lin
authors.initials: ZL
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D020816:Amino Acid Motifs; D019380:Anti-HIV Agents / Q000494:pharmacology; D024882:Drug Resistance, Viral; D014644:Genetic Variation; D015497:HIV-1 / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D019380:Anti-HIV Agents; D054299:env Gene Products, Human Immunodeficiency Virus