title: Activated T cells induce rapid CD83 expression on B cells by engagement of CD40.
abstract: The conserved transmembrane glycoprotein CD83 was originally described as highly specific marker for mature dendritic cells in the peripheral circulation. Besides its regulatory role in thymic T cell maturation and peripheral T cell activation, recent studies suggest, that CD83 is also involved in the regulation of B cell maturation, homeostasis and function. Here we show, that antigen-specific T cell stimulation leads to CD83 induction predominantly on B cells. In vivo activation of T cells by injection of cognate antigenic peptide into T cell receptor transgenic mice induced strong expression of the early activation marker CD69 but only low levels of surface CD83 on T cells. In contrast CD83 was induced on 80% of B cells in the draining lymph node. This T cell mediated induction of CD83 expression on B cells was not mediated by soluble factors but was contact dependent because separation of B cells from an ongoing T cell stimulation in a transwell system abrogated CD83 expression. Since CD83 expression was induced on both MHC-matched and MHC-mismatched B cells present in cultures of activated T cells, cell contact via TCR/MHC binding was not essential. The application of an antibody to the CD40 ligand of T cells, however, strongly interfered with the induction of CD83 expression on bystander B cells. Taken together we provide evidence that activated T cells induce CD83 on B cells via CD40 engagement but independent of TCR/MHC binding and thus independent of antigen-specificity of B cells.
authors.affiliation: Department of Immunology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany. birte.kretschmer@bni-hamburg.de
authors.forename: Birte
authors.initials: B
authors.lastname: Kretschmer
authors.forename: Svenja
authors.initials: S
authors.lastname: Kühl
authors.forename: Bernhard
authors.initials: B
authors.lastname: Fleischer
authors.forename: Minka
authors.initials: M
authors.lastname: Breloer
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D019013:CD40 Antigens / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D006649:Histocompatibility Antigens / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011485:Protein Binding; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology; D000097802:CD83 Antigen
chemical_list: D000941:Antigens; D015703:Antigens, CD; D019013:CD40 Antigens; D000939:Epitopes; D006649:Histocompatibility Antigens; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D011948:Receptors, Antigen, T-Cell; D000097802:CD83 Antigen