title: Hydrocortisone enhances the function of the blood-nerve barrier through the up-regulation of claudin-5.
abstract: In autoimmune disorders of the peripheral nervous system (PNS), including Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered to be a key step in the disease process. Although glucocorticoids (GCs) have been shown to effectively restore the blood-brain barrier (BBB) in some inflammatory central nervous system diseases such as multiple sclerosis, their action against the BNB has not yet been examined. To elucidate the role of GCs on the BNB, we established a novel human immortalized endothelial cell lines derived from the BNB. The established cell line termed "DH-BNBs" expresses two important tight junction proteins, claudin-5 and occludin. Using DH-BNBs, we analyzed how GCs affect BNB function. We herein report that GCs up-regulate the expression of claudin-5 and increase the barrier properties of the BNB. This is the first report which indicates how GCs affect the blood-nerve barrier.
authors.affiliation: Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 7558505, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kashiwamura
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Sano
authors.forename: Masaaki
authors.initials: M
authors.lastname: Abe
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Shimizu
authors.forename: Hiroyo
authors.initials: H
authors.lastname: Haruki
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Maeda
authors.forename: Motoharu
authors.initials: M
authors.lastname: Kawai
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanda
mesh_terms: D001483:Base Sequence; D049428:Blood-Nerve Barrier / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D002461:Cell Line, Transformed; D062507:Claudin-5; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D008024:Ligands; D008565:Membrane Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D019108:Tight Junctions / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: C107466:CLDN5 protein, human; D062507:Claudin-5; D017931:DNA Primers; D008024:Ligands; D008565:Membrane Proteins; D012333:RNA, Messenger; D006854:Hydrocortisone