title: A colorimetric assay for determination of residual detergent levels in reconstituted membrane protein preparations.
abstract: Simple and robust methods for the quantification of residual detergent in purified membrane proteins are not readily available. In this article, solubilization of precipitated dye by detergent is shown to be a facile method for the quantification of residual levels of octaethylene-glycol-mono(n-dodecyl)ether in virosomal influenza vaccine. Dye solubilization starts in the critical micellar concentration range. The method is more sensitive than an existing assay and is highly accurate and precise. The method is applicable to other detergents as well. This method of residual detergent quantification is simple and straightforward and is a useful tool for quality control of subunit vaccines.
authors.affiliation: Abbott Biologicals, 1380 DA Weesp, The Netherlands.
authors.forename: Rens
authors.initials: R
authors.lastname: Roosloot
authors.forename: Pieter
authors.initials: P
authors.lastname: Schoen
mesh_terms: D003124:Colorimetry / Q000379:methods*; D005026:Ethylene Glycols / Q000032:analysis*; D007252:Influenza Vaccines / Q000737:chemistry / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry*; D022223:Vaccines, Subunit / Q000737:chemistry / Q000276:immunology
chemical_list: D005026:Ethylene Glycols; D007252:Influenza Vaccines; D008565:Membrane Proteins; D022223:Vaccines, Subunit; C519924:octaethylene glycol monotetradecyl ether