title: CE-MS in biomarker discovery, validation, and clinical application.
abstract: CE coupled MS (CE-MS) has become an increasingly employed technology in proteome analysis with focus on the identification of biomarker peptides in clinical proteomics. In this review, we will cover technical aspects of CE-MS coupling and highlight the improvements made in the last few years. We examine CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. We discuss the principal theoretical and practical obstacles encountered when employing CE-MS (and most other proteomic technologies) for the analysis of body fluids for biomarker discovery. We will present several examples of a successful application of CE-MS for biomarker discovery, implications for disease diagnosis, prognosis, and therapy evaluation, and will discuss current challenges and possible future improvements.
authors.affiliation: Mosaiques Diagnostics & Therapeutics, Hannover, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Mischak
authors.forename: Joost P
authors.initials: JP
authors.lastname: Schanstra
mesh_terms: D000375:Aging / Q000652:urine; D015415:Biomarkers / Q000032:analysis* / Q000652:urine; D003324:Coronary Artery Disease / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007674:Kidney Diseases / Q000652:urine; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods
chemical_list: D015415:Biomarkers