title: Involvement of cyclophilin B in the replication of Japanese encephalitis virus.
abstract: Japanese encephalitis virus (JEV) is a mosquito-borne RNA virus that belongs to the Flaviviridae family. In this study, we have examined the effect of cyclosporin A (CsA) on the propagation of JEV. CsA exhibited potent anti-JEV activity in various mammalian cell lines through the inhibition of CypB. The propagation of JEV was impaired in the CypB-knockdown cells and this reduction was cancelled by the expression of wild-type but not of peptidylprolyl cis-trans isomerase (PPIase)-deficient CypB, indicating that PPIase activity of CypB is critical for JEV propagation. Infection of pseudotype viruses bearing JEV envelope proteins was not impaired by the knockdown of CypB, suggesting that CypB participates in the replication but not in the entry of JEV. CypB was colocalized and immunoprecipitated with JEV NS4A in infected cells. These results suggest that CypB plays a crucial role in the replication of JEV through an interaction with NS4A.
authors.affiliation: Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka, Japan
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kambara
authors.forename: Hideki
authors.initials: H
authors.lastname: Tani
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Mori
authors.forename: Takayuki
authors.initials: T
authors.lastname: Abe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Katoh
authors.forename: Takasuke
authors.initials: T
authors.lastname: Fukuhara
authors.forename: Shuhei
authors.initials: S
authors.lastname: Taguwa
authors.forename: Kohji
authors.initials: K
authors.lastname: Moriishi
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Matsuura
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D021983:Cyclophilins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology; D004664:Encephalitis Virus, Japanese / Q000187:drug effects / Q000502:physiology*; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D000998:Antiviral Agents; C080106:NS4A protein, flavivirus; D017361:Viral Nonstructural Proteins; C081786:cyclophilin B; D016572:Cyclosporine; D021983:Cyclophilins