title: [Effect of combined use of rehmannia and rhodiola on hemopoietic function in mice with bone marrow depression].
abstract: OBJECTIVE To explore the effect of combined use of rehmannia (RM) and rhodiola (RD) on peripheral leukopenia and bone marrow hematopoietic function suppression induced by cyclophosphamide (CTX) in mice.
  
  
  METHODS
  ICR mice were established into bone marrow inhibition models by intraperitoneal injection of CTX, and were administered with RM, RD or its extract (RDE), singly or in mixture, via gastrogavage for 10 days. The changes of peripheral hemogram, bone marrow nucleated cell proliferation, CFU-GM colony formation, GM-CSF and erythropoietin (EPO) secretion were observed.
  
  
  RESULTS
  Compared with the un-treated model mice, the peripheral white blood cell count was significantly higher in model mice treated with RDE and RM mixture; the bone marrow nucleated cells count, CFU-GM formation, and GM-CSF production were significant higher in model mice treated with RD and RM mixture, showing statistical significance (P < 0.01); while EPO production in the RD and RM mixture treated group was slightly elevated, but the difference showed no statistical significance.
  
  
  CONCLUSIONS
  RD and RM mixture could regulate hematopoietic system by promoting the production of bone marrow cells and colonies, as well as enhancing the synthesis of related cytokines, such as GM-CSF, so as to increase the amount of peripheral white blood cells and restore the hematopoietic function of organism.
authors.affiliation: Changchun University of Traditional Chinese Medicine, Changchun. jingluo304@yahoo.com.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lin
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D003520:Cyclophosphamide; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004921:Erythropoietin / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects*; D007970:Leukopenia / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D032267:Rehmannia / Q000737:chemistry*; D029771:Rhodiola / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003520:Cyclophosphamide