title: Oligonucleotide array-in-well platform for detection and genotyping human adenoviruses by utilizing upconverting phosphor label technology.
abstract: We have developed a robust array-in-well test platform based on an oligonucleotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.
authors.affiliation: Department of Biotechnology, University of Turku, Turku, Finland. minna.yliharsila@utu.fi
authors.forename: Minna
authors.initials: M
authors.lastname: Ylihärsilä
authors.forename: Timo
authors.initials: T
authors.lastname: Valta
authors.forename: Maija
authors.initials: M
authors.lastname: Karp
authors.forename: Liisa
authors.initials: L
authors.lastname: Hattara
authors.forename: Emilia
authors.initials: E
authors.lastname: Harju
authors.forename: Jorma
authors.initials: J
authors.lastname: Hölsä
authors.forename: Petri
authors.initials: P
authors.lastname: Saviranta
authors.forename: Matti
authors.initials: M
authors.lastname: Waris
authors.forename: Tero
authors.initials: T
authors.lastname: Soukka
mesh_terms: D000042:Absorption; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D005240:Feasibility Studies; D005838:Genotype; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D028581:Lanthanoid Series Elements / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D009305:Nasopharynx / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D010316:Particle Size; D017785:Photons; D015203:Reproducibility of Results
chemical_list: D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D015345:Oligonucleotide Probes