title: Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells.
abstract: Yeast is a model eukaryote with a variety of biological resources. Here we developed a method to track a quantum dot (QD)-conjugated protein in the budding yeast Saccharomyces cerevisiae. We chemically conjugated QDs with the yeast prion Sup35, incorporated them into yeast spheroplasts, and tracked the motions by conventional two-dimensional or three-dimensional tracking microscopy. The method paves the way toward the individual tracking of proteins of interest inside living yeast cells.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Biosciences and Biotechnology, Tokyo Institute of Technology, B56, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Tsuji
authors.forename: Shigeko
authors.initials: S
authors.lastname: Kawai-Noma
authors.forename: Chan-Gi
authors.initials: CG
authors.lastname: Pack
authors.forename: Hideki
authors.initials: H
authors.lastname: Terajima
authors.forename: Junichiro
authors.initials: J
authors.lastname: Yajima
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nishizaka
authors.forename: Masataka
authors.initials: M
authors.lastname: Kinjo
authors.forename: Hideki
authors.initials: H
authors.lastname: Taguchi
mesh_terms: D008856:Microscopy, Fluorescence; D010454:Peptide Termination Factors / Q000032:analysis / Q000378:metabolism*; D021381:Protein Transport; D045663:Quantum Dots; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D010454:Peptide Termination Factors; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins