title: Circulating cell-derived microparticles in women with pregnancy loss.
abstract: OBJECTIVE
  To analyze cell-derived microparticles (cMP) in pregnancy loss (PL), both recurrent miscarriages (RM) and unexplained fetal loss (UFL).
  
  
  METHODS
  Non-matched case-control study was performed at Vall d'Hebron Hospital. Cell-derived microparticles of 53 PL cases, 30 with RM, 16 with UFL, and 7 (RM + UFL), were compared to 38 healthy pregnant women. Twenty healthy non-pregnant women act as controls. Cell-derived microparticles were analyzed through flow cytometry. Results are given as total annexin (A5+), endothelial-(CD144+/CD31+ CD41-), platelet-(CD41+), leukocyte-(CD45+) and CD41- c-MP/μL of plasma. Antiphospholipid antibodies (aPLA) were analyzed according to established methods.
  
  
  RESULTS
  Comparing PL versus healthy pregnant, we observed a significant endothelial cMP decrease in PL. When comparing RM subgroup with controls, we observed significant decreases in endothelial cMP. When comparing the PL positive for aPLA versus PL-aPLA-negative, no cMP numbering differences were seen.
  
  
  CONCLUSIONS
  Pregnancy loss seems to be related to endothelial cell activation and/or consumption. A relationship between aPLA and cMP could not be demonstrated.
authors.affiliation: Ageing and Systemic Autoimmune Disease Unit, Department of Internal Medicine I, Vall d'Hebron University Hospital, Faculty of Medicine, Universitat Autònoma, Barcelona, Spain. 16297jar@comb.es
authors.forename: Jaume
authors.initials: J
authors.lastname: Alijotas-Reig
authors.forename: Carles
authors.initials: C
authors.lastname: Palacio-Garcia
authors.forename: Immaculada
authors.initials: I
authors.lastname: Farran-Codina
authors.forename: Cristina
authors.initials: C
authors.lastname: Zarzoso
authors.forename: Luis
authors.initials: L
authors.lastname: Cabero-Roura
authors.forename: Miquel
authors.initials: M
authors.lastname: Vilardell-Tarres
mesh_terms: D000026:Abortion, Habitual / Q000097:blood*; D000328:Adult; D017302:Annexins / Q000097:blood; D017152:Antibodies, Antiphospholipid / Q000097:blood; D015703:Antigens, CD / Q000097:blood; D001792:Blood Platelets / Q000166:cytology; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D011247:Pregnancy
chemical_list: D017302:Annexins; D017152:Antibodies, Antiphospholipid; D015703:Antigens, CD; D003176:Complement C3; D003181:Complement C4