title: Inhibitory effects of a tryptamine derivative on ultraviolet radiation-induced apoptosis in MC3T3-E1 mouse osteoblasts.
abstract: MS-IPA1 is a new synthetic compound that is synthesized from tryptamine. Recently, our group demonstrated that SST-VED-I-1, which has a similar chemical structure to MS-IPA1, inhibits starvation-induced apoptosis in osteoblasts. However, the effects of MS-IPA1 on apoptosis in osteoblasts have not yet been examined. Therefore, this study examined the effects of this compound on apoptosis in osteoblasts. In this study, MC3T3-E1 mouse osteoblasts were used and apoptosis was induced by ultraviolet radiation (UV). We investigated the effect of MS-IPA1 on apoptosis by analyzing caspase3/7 activity, translocation of phosphatidylserine (PS), and mRNA expression levels of Bcl-2 and Bax. In addition, it was investigated whether MS-IPA1 affects cell proliferation and cell cycle progression. We found that MS-IPA1 had no effect on cell proliferation or cell cycle progression. However, MS-IPA1 suppressed UV-induced cell death in a dose-dependent manner, which was accompanied with the inhibition of caspase activation and translocation of PS. Furthermore, after UV exposure, Bcl-2 expression was increased in the MS-IPA1-treated cells as compared to that in the vehicle-treated cells. In contrast, Bax expression was decreased in the MS-IPA1-treated cell as compared to that in the vehicle-treated cells. These results suggest that MS-IPA1 has an inhibitory effect on apoptosis in osteoblasts through a Bcl-2 family-dependent signaling pathway.
authors.affiliation: Department of Anatomy, Nihon University School of Dentistry, Tokyo 101-8310, Japan. mikami-t@dent.nihon-u.ac.jp
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Mikami
authors.forename: Motoki
authors.initials: M
authors.lastname: Senoo
authors.forename: Mio
authors.initials: M
authors.lastname: Lee
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yamada
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Ochiai
authors.forename: Masaki J
authors.initials: MJ
authors.lastname: Honda
authors.forename: Eri
authors.initials: E
authors.lastname: Watanabe
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Watanabe
authors.forename: Masanori
authors.initials: M
authors.lastname: Somei
authors.forename: Minoru
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000818:Animals; D017302:Annexins / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D015398:Signal Transduction / Q000187:drug effects; D014363:Tryptamines / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: C560842:1-hydroxy-N-methoxycarbonyltryptamine; D017302:Annexins; D014363:Tryptamines; D053148:Caspase 3