title: Use of adipose tissue-derived stromal cells for prevention of esophageal stricture after circumferential EMR in a canine model.
abstract: BACKGROUND
  EMR is an accepted treatment for early esophageal carcinoma. However, resection of a large mucosal area often causes postoperative esophageal stricture.
  
  
  OBJECTIVE
  To investigate the efficacy of autologous adipose tissue-derived stromal cells (ADSCs) for prevention of stricture formation after EMR in dogs.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  University research center.
  
  
  METHODS
  Ten beagle dogs were randomized into a control group and an ADSCs-injected (ADSC) group. The ADSCs were isolated from autologous adipose tissue. Immediately after circumferential esophageal EMR, about 5 × 10(6) ADSCs suspended in 8 mL of phosphate-buffered saline solution were injected endoscopically into the residual submucosa of the ADSC group, whereas the control group received only 8 mL of phosphate-buffered saline solution.
  
  
  METHODS
  Dysphagia score, weight loss, rate of mucosal constriction, and histologic assessments.
  
  
  RESULTS
  In the control and ADSC groups, the median dysphagia scores were 4 and 1 (P < .043), the mean degrees of mucosal constriction were 75.7% and 45.3% (P < .008), and the numbers of nascent microvessels in the submucosal layer were 7.4 and 16.2 per unit area (P = .007), respectively. Atrophy and fibrosis of the muscularis propria layer were observed in the control group.
  
  
  CONCLUSIONS
  Animal study, small sample size.
  
  
  CONCLUSIONS
  Injection therapy with autologous ADSCs suppresses constriction of the esophageal mucosa and improves clinical symptoms after circumferential EMR in this canine model.
authors.affiliation: Department of Bioartificial Organs, Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan.
authors.forename: Michitaka
authors.initials: M
authors.lastname: Honda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hori
authors.forename: Akira
authors.initials: A
authors.lastname: Nakada
authors.forename: Masato
authors.initials: M
authors.lastname: Uji
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nishizawa
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Shimada
authors.forename: Naoki
authors.initials: N
authors.lastname: Kida
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Sato
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D000818:Animals; D004285:Dogs; D004940:Esophageal Stenosis / Q000209:etiology / Q000517:prevention & control*; D016629:Esophagectomy / Q000009:adverse effects* / Q000379:methods; D004947:Esophagus / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D007267:Injections; D007413:Intestinal Mucosa / Q000601:surgery*; D008297:Male; D011183:Postoperative Complications; D017154:Stromal Cells / Q000637:transplantation*; D014182:Transplantation, Autologous / Q000379:methods; D016896:Treatment Outcome