title: The effects of growth and differentiation factor 5 on bone marrow stromal cell transplants in an in vitro tendon healing model.
abstract: The effects of growth differentiation factor-5 (GDF-5) and bone marrow stromal cells (BMSCs) on tendon healing were investigated under in vitro tissue culture conditions. BMSCs and GDF-5 placed in a collagen gel were interpositioned between the cut ends of dog flexor digitorum profundus tendons. The tendons were randomly assigned into four groups: 1) repaired tendon without gel; 2) repaired tendon with BMSC-seeded gel; 3) repaired tendon with GDF-5 gel without cells; and 4) repaired tendon with GDF-5 treated BMSC-seeded gel. At 2 and 4 weeks, the maximal strength of repaired tendons with GDF-5 treated BMSCs-seeded gel was significantly higher than in tendons without gel interposition. However, neither BMSCs nor GDF-5 alone significantly increased the maximal strength of healing tendons at 2 or 4 weeks. These results suggest that the combination of BMSCs and GDF-5 accelerates tendon healing, but either BMSCs or GDF-5 alone are not effective in this model.
authors.affiliation: Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, Rochester, MN, USA.
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.forename: K-N
authors.initials: KN
authors.lastname: An
authors.forename: P C
authors.initials: PC
authors.lastname: Amadio
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D004285:Dogs; D055428:Growth Differentiation Factor 5 / Q000627:therapeutic use*; D023421:Models, Animal; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000637:transplantation*; D013708:Tendon Injuries / Q000601:surgery*; D046509:Tissue Culture Techniques; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D055428:Growth Differentiation Factor 5