title: Mesenchymal stem cells and platelet lysate in fibrin or collagen scaffold promote non-cemented hip prosthesis integration.
abstract: The objective of this study was to evaluate whether mesenchymal stem cells (MSC) and platelet lysate (PL) seeded in a fibrin or collagen scaffold could improve the new bone (NB) formation around an uncemented hip prosthesis stem in a sheep model. In vitro expanded MSC were suspended in PL and either mixed with collagen or fibrin gel as delivery vehicle. The cell-gel composites were inserted inside the femoral canal, then the prosthesis was press-fit inserted inside the femur. Identical procedures were performed in a control group, but only the prosthesis was implanted. Histomorphometrical analysis performed 4 months after surgery indicated that the newly formed bone inside the medullary canal, between the inner cortex and the prosthetic stem, was significantly higher in the MSC-PL-collagen group (mean 18.7 ± 4.5%) and in the MSC-PL-fibrin group (mean 18.8 ± 15.2%) when compared to the control group (mean 4.6 ± 2.0%). There was a significantly higher bone-prosthesis contact in the MSC-PL-collagen group (mean 2.7 ± 2.6%) and in the MSC-PL-fibrin group (mean 2.3 ± 3.1%) compared to the control group (mean 0.2 ± 0.1%). The results indicate that MSC and PL in a fibrin or collagen scaffold can promote NB formation around an uncemented hip prosthesis stem.
authors.affiliation: Bone Regeneration Laboratory, Rizzoli Orthopaedic Institute, 40136 Bologna, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dozza
authors.forename: Claudia
authors.initials: C
authors.lastname: Di Bella
authors.forename: Enrico
authors.initials: E
authors.lastname: Lucarelli
authors.forename: Gianluca
authors.initials: G
authors.lastname: Giavaresi
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Tazzari
authors.forename: Sandro
authors.initials: S
authors.lastname: Giannini
authors.forename: Davide
authors.initials: D
authors.lastname: Donati
mesh_terms: D000818:Animals; D019644:Arthroplasty, Replacement, Hip; D001792:Blood Platelets; D003094:Collagen; D005260:Female; D005269:Femur / Q000033:anatomy & histology; D005337:Fibrin; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis; D010983:Plateletpheresis; D012756:Sheep; D054457:Tissue Scaffolds; D014025:Titanium
chemical_list: D005337:Fibrin; D003094:Collagen; D014025:Titanium