title: Classification of modern and old Río Tinto sedimentary deposits through the biomolecular record using a life marker biochip: implications for detecting life on Mars.
abstract: The particular mineralogy formed in the acidic conditions of the Río Tinto has proven to be a first-order analogue for the acid-sulfate aqueous environments of Mars. Therefore, studies about the formation and preservation of biosignatures in the Río Tinto will provide insights into equivalent processes on Mars. We characterized the biomolecular patterns recorded in samples of modern and old fluvial sediments along a segment of the river by means of an antibody microarray containing more than 200 antibodies (LDCHIP200, for Life Detector Chip) against whole microorganisms, universal biomolecules, or environmental extracts. Samples containing 0.3-0.5 g of solid material were automatically analyzed in situ by the Signs Of LIfe Detector instrument (SOLID2), and the results were corroborated by extensive analysis in the laboratory. Positive antigen-antibody reactions indicated the presence of microbial strains or high-molecular-weight biopolymers that originated from them. The LDCHIP200 results were quantified and subjected to a multivariate analysis for immunoprofiling. We associated similar immunopatterns, and biomolecular markers, to samples with similar sedimentary age. Phyllosilicate-rich samples from modern fluvial sediments gave strong positive reactions with antibodies against bacteria of the genus Acidithiobacillus and against biochemical extracts from Río Tinto sediments and biofilms. These samples contained high amounts of sugars (mostly polysaccharides) with monosaccharides like glucose, rhamnose, fucose, and so on. By contrast, the older deposits, which are a mix of clastic sands and evaporites, showed only a few positives with LDCHIP200, consistent with lower protein and sugar content. We conclude that LDCHIP200 results can establish a correlation between microenvironments, diagenetic stages, and age with the biomarker profile associated with a sample. Our results would help in the search for putative martian biomarkers in acidic deposits with similar diagenetic maturity. Our LDCHIP200 and SOLID-like instruments may be excellent tools for the search for molecular biomarkers on Mars or other planets.
authors.affiliation: Centro de Astrobiología (INTA-CSIC), Madrid, Spain. parrogv@inta.es
authors.forename: Victor
authors.initials: V
authors.lastname: Parro
authors.forename: David
authors.initials: D
authors.lastname: Fernández-Remolar
authors.forename: José A
authors.initials: JA
authors.lastname: Rodríguez-Manfredi
authors.forename: Patricia
authors.initials: P
authors.lastname: Cruz-Gil
authors.forename: Luis A
authors.initials: LA
authors.lastname: Rivas
authors.forename: Marta
authors.initials: M
authors.lastname: Ruiz-Bermejo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Moreno-Paz
authors.forename: Miriam
authors.initials: M
authors.lastname: García-Villadangos
authors.forename: David
authors.initials: D
authors.lastname: Gómez-Ortiz
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Blanco-López
authors.forename: César
authors.initials: C
authors.lastname: Menor-Salván
authors.forename: Olga
authors.initials: O
authors.lastname: Prieto-Ballesteros
authors.forename: Javier
authors.initials: J
authors.lastname: Gómez-Elvira
mesh_terms: D015415:Biomarkers / Q000032:analysis; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D016000:Cluster Analysis; D017753:Ecosystem; D018559:Exobiology / Q000379:methods*; D005118:Extraterrestrial Environment / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000145:classification*; D018535:Mars; D008903:Minerals / Q000032:analysis; D015999:Multivariate Analysis; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D002241:Carbohydrates; D008903:Minerals; D011506:Proteins