title: Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method.
abstract: Encapsulation of probiotic bacteria in cross-linked alginate beads is of major interest for improving the survivability in harsh acid and bile environment and also in food matrices. Alginate micro beads (10-40 μm) containing the probiotics Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM were produced by a novel technique based on dual aerosols of alginate solution and CaCl(2) cross linking solution. Extruded macro beads (approximately 2mm diameter) produced by the conventional method and micro beads produced by novel aerosols technique offered comparable protection to L. rhamnosus in high acid and bile environment. Chitosan coating of micro beads resulted in a significant increase in survival time of L. rhamnosus from 40 to 120 min in acid condition and the reduction in cell numbers was confined to 0.94 log over this time. Alginate macro beads are more effective than micro beads in protecting L. acidophilus against high acid and bile. Chitosan coating of micro beads resulted in similar protection to L. acidophilus in macro beads in acid and extended the survival time from 90 to at least 120 min. Viability of this organism in micro beads was 3.5 log after 120 min. The continuous processing capability and scale-up potential of the dual aerosol technique offers potential for an efficient encapsulation of probiotics in very small alginate micro beads below sensorial detection limits while still being able to confer effective protection in acid and bile environment.
authors.affiliation: School of Agriculture and Food Sciences, University of Queensland, St Lucia 4072, Australia.
authors.forename: Asma
authors.initials: A
authors.lastname: Sohail
authors.forename: Mark S
authors.initials: MS
authors.lastname: Turner
authors.forename: Allan
authors.initials: A
authors.lastname: Coombes
authors.forename: Thor
authors.initials: T
authors.lastname: Bostrom
authors.forename: Bhesh
authors.initials: B
authors.lastname: Bhandari
mesh_terms: D000143:Acids / Q000494:pharmacology; D000336:Aerosols; D000464:Alginates / Q000737:chemistry*; D001646:Bile / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D003432:Cross-Linking Reagents; D005782:Gels / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000254:growth & development*; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects / Q000254:growth & development*; D050296:Microbial Viability; D008863:Microspheres; D019936:Probiotics
chemical_list: D000143:Acids; D000336:Aerosols; D000464:Alginates; D003432:Cross-Linking Reagents; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan