title: Enhanced secreting expression and improved properties of a recombinant alkaline endoglucanase cloned in Escherichia coli.
abstract: An alkaline endoglucanase from Bacillus akibai III-3A was successfully expressed in Escherichia coli in active form, and secretion was greatly enhanced by addition of 5 g/l ethylenediamine tetraacetic acid (EDTA) to the culture medium at the induction time of 12 h. Under the optimal culture conditions, extracellular and total endoglucanase activities were 18.5 and 31.2 U/ml, respectively. Both the recombinant and native enzymes exhibited similar properties with respect to broad pH stability, good thermostability, and resistibility to various metal ions and reagents examined. However, unlike the native endoglucanase that was partly inhibited by sodium dodecyl sulfate (SDS), the recombinant enzyme had good resistibility to SDS, being very stable in the commercial detergents, and no decrease in residual activity was observed in 0.2% (w/v) laundry detergent, indicating that it was suitable for application in detergents industry.
authors.affiliation: Shenzhen Key Laboratory for Microbial Gene Engineering, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China. liulsl@szu.edu.cn
authors.forename: Sen-Lin
authors.initials: SL
authors.lastname: Liu
authors.forename: Wei-Zhao
authors.initials: WZ
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Miao
authors.initials: M
authors.lastname: Xing
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003902:Detergents / Q000737:chemistry; D004492:Edetic Acid / Q000494:pharmacology; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D011994:Recombinant Proteins; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid; D002480:Cellulase