title: Apoptosis-related mRNA expression profiles of ovarian cancer cell lines following cisplatin treatment.
abstract: OBJECTIVE
  The aim of this study was to identify apoptosis-related genes of ovarian cancer cell lines following cisplatin treatment.
  
  
  METHODS
  We used IC(50) values and fluorescence-activated cell sorting analysis to compare cell death in 2 ovarian cancer cell lines, namely, SKOV-3 and OVCAR-3, upon treatment with cisplatin. Moreover, the change in transcriptional levels of apoptosis-associated genes was measured with a dendron-modified DNA microarray.
  
  
  RESULTS
  The protein levels for the up-regulated genes in each cell line were validated to identify the molecules that may determine the cellular behavior of cisplatin resistance. Eight genes were over-expressed in the 2 cell lines. The cisplatin-induced up-regulation of DAD1 in transcriptional and protein levels contributed to the cisplatin resistance of OVCAR-3, and the up-regulation of FASTK and TNFRSF11A in SKOV-3 resulted in its higher sensitivity to cisplatin than that of OVCAR-3.
  
  
  CONCLUSIONS
  In the present study, we have identified a set of genes responsible for apoptosis following cisplatin treatment in ovarian cancer cell lines. These genes may give information about the understanding of cisplatin-induced apoptosis in ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, St. Vincent's Hospital, The Catholic University of Korea, Suwon, Korea.
authors.forename: Joohee
authors.initials: J
authors.lastname: Yoon
authors.forename: Eung-Sam
authors.initials: ES
authors.lastname: Kim
authors.forename: Sung Jong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Chang-Wook
authors.initials: CW
authors.lastname: Park
authors.forename: Hyung Jin
authors.initials: HJ
authors.lastname: Cha
authors.forename: Bee Hak
authors.initials: BH
authors.lastname: Hong
authors.forename: Kwan Yong
authors.initials: KY
authors.lastname: Choi