title: Immunohistochemical analysis of beta-defensin-2 expression in human lung tumors.
abstract: OBJECTIVE
  The present research was directed on analysis of the expression patterns of human beta-defensin-2 (hBD-2) in human lung tumors.
  
  
  METHODS
  Specimens of surgically resected human lung tumors (n = 31) of different histological type (1 case of small cell lung cancer, and 30 cases of non-small cell lung cancer (1 case of clear cell carcinoma, 9 cases of squamous cell carcinoma (SCC), and 20 cases of adenocarcinoma (AC)) were analyzed for expression of hBD-2 with the use of immunohistochemical analysis.
  
  
  RESULTS
  Immunohistochemical analysis has revealed that all lung tumor samples independently on their histological type express hBD-2 peptide, however at different levels (from < 5% to 100% cells). According to our observations, low-differentiated AC differs from moderately differentiated AC by significantly lower hBD-2 expression levels (p < 0.05). No correlation between hBD-2 expression patterns and PCNA or Bcl-2 expression has been found.
  
  
  CONCLUSIONS
  Human beta-defensin-2 expression levels may depend on differentiation grade of lung adenocarcinoma.
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Vasylkivska str. 45, Kyiv 03022, Ukraine.
authors.forename: T
authors.initials: T
authors.lastname: Shestakova
authors.forename: E
authors.initials: E
authors.lastname: Zhuravel
authors.forename: L
authors.initials: L
authors.lastname: Bolgova
authors.forename: S
authors.initials: S
authors.lastname: Zaitsev
authors.forename: O
authors.initials: O
authors.lastname: Efanova
authors.forename: M
authors.initials: M
authors.lastname: Soldatkina
authors.forename: P
authors.initials: P
authors.lastname: Pogrebnoy
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D055752:Small Cell Lung Carcinoma / Q000378:metabolism* / Q000473:pathology; D023083:beta-Defensins / Q000096:biosynthesis*
chemical_list: C106641:DEFB4A protein, human; D023083:beta-Defensins