title: Glycomimicry: display of fucosylation on the lipo-oligosaccharide of recombinant Escherichia coli K12.
abstract: We recently described the design of Escherichia coli K12 and Salmonella enterica sv Typhimurium to display the gangliomannoside 3 (GM3) antigen on the cell surface. We report here the fucosylation of modified lipooligosaccharide in a recombinant E.coli strain with a truncated lipid A core due to deletion of the core glycosyltransferases genes waaO and waaB. This truncated structure was used as a scaffold to assemble the Lewis Y motif by consequent action of the heterologously expressed β-1,4 galactosyltransferase LgtE (Neisseria gonorrheae), the β-1,3 N-acetylglucosaminyltransferase LgtA and the β-1,3 galactosyltransferase LgtB from Neisseria meningitidis, as well as the α-1,2 and α-1,3 fucosyltransferases FutC and FutA from Helicobacter pylori. We show the display of the Lewis Y structure by immunological and chemical analysis.
authors.affiliation: CERMAV-CNRS, Grenoble, France.
authors.forename: Elif
authors.initials: E
authors.lastname: Yavuz
authors.forename: Carola
authors.initials: C
authors.lastname: Maffioli
authors.forename: Karin
authors.initials: K
authors.lastname: Ilg
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
authors.forename: Bernard
authors.initials: B
authors.lastname: Priem
mesh_terms: D001483:Base Sequence; D002240:Carbohydrate Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D048168:Escherichia coli K12 / Q000235:genetics / Q000378:metabolism*; D005643:Fucose / Q000378:metabolism*; D050356:Lipid Metabolism; D008745:Methylation; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D011995:Recombination, Genetic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017931:DNA Primers; D009844:Oligosaccharides; D005643:Fucose