title: Essential role for the interaction between hnRNP H/F and a G quadruplex in maintaining p53 pre-mRNA 3'-end processing and function during DNA damage.
abstract: Following DNA damage, mRNA 3'-end formation is inhibited, contributing to repression of mRNA synthesis. Here we investigated how DNA-damaged cells accomplish p53 mRNA 3'-end formation when normal mechanisms of pre-mRNA 3'-end processing regulation are inhibited. The underlying mechanism involves the interaction between a G-quadruplex structure located downstream from the p53 cleavage site and hnRNP H/F. Importantly, this interaction is critical for p53 expression and contributes to p53-mediated apoptosis. Our results uncover the existence of a specific rescue mechanism of 3'-end processing regulation allowing stress-induced p53 accumulation and function in apoptosis.
authors.affiliation: INSERM U563, Institut Claudius Regaud, Toulouse, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Decorsière
authors.forename: Anne
authors.initials: A
authors.lastname: Cayrel
authors.forename: Stéphan
authors.initials: S
authors.lastname: Vagner
authors.forename: Stefania
authors.initials: S
authors.lastname: Millevoi
mesh_terms: D017209:Apoptosis / Q000502:physiology; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000235:genetics* / Q000528:radiation effects; D054856:G-Quadruplexes; D005786:Gene Expression Regulation; D045325:HCT116 Cells; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D026721:RNA 3' End Processing / Q000235:genetics*; D012322:RNA Precursors / Q000378:metabolism*; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H; D012322:RNA Precursors; D016159:Tumor Suppressor Protein p53