title: Fractionation and recovery of whey proteins by hydrophobic interaction chromatography.
abstract: A method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1 M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic strength of the buffer using a salt gradient. The results showed that the most hydrophobic protein from whey is α-lactalbumin and the less hydrophobic is lactoferrin. It was possible to recover 45.2% of β-lactoglobulin using the HiPrep Octyl Sepharose FF column from the whey protein concentrate mixture with 99.6% purity on total protein basis.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Santos
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.forename: Lígia R
authors.initials: LR
authors.lastname: Rodrigues
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D057927:Hydrophobic and Hydrophilic Interactions; D008894:Milk Proteins / Q000737:chemistry / Q000302:isolation & purification*; D009994:Osmolar Concentration; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins; D000645:Ammonium Sulfate