title: Rapid multiplex PCR assay for the simultaneous detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis.
abstract: The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratoryacquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.
authors.affiliation: Division of Biotechnology, Defence Research and Development Establishment, Jhansi Road, Gwalior, Madhya Pradesh, India. drksanjay78@gmail.com
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Urmil
authors.initials: U
authors.lastname: Tuteja
authors.forename: Kumari
authors.initials: K
authors.lastname: Sarika
authors.forename: Dhirendra
authors.initials: D
authors.lastname: Singh
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.forename: Om
authors.initials: O
authors.lastname: Kumar
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002002:Brucella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial