title: White tea (Camellia sinensis Kuntze) exerts neuroprotection against hydrogen peroxide-induced toxicity in PC12 cells.
abstract: Tea is a popular beverage whose consumption is associated with prevention of certain disorders. The objective of the study was to investigate the potential neuroprotective effect of white tea extract (WTE) on hydrogen peroxide induced toxicity in PC12 cells. Cells were treated with various doses of WTE (10-250 μg/ml) before exposition to 250 μM hydrogen peroxide and cell survival was determined through the MTT and LDH assays. Oxidative stress was quantified in the cells after treatments as intracellular reactive oxygen species (ROS) production and the antioxidant activity of the extract was assessed in a cell free system in terms of free radical scavenging capacity. Results showed that WTE has a significant protective effect in the PC12 cell line against hydrogen peroxide as cell survival was significantly superior in WTE-treated cells compared to hydrogen peroxide-treated cells. A reduction on intracellular oxidative stress as well as radical scavenging properties were produced by WTE. Results suggest that WTE protects PC12 cells against H(2)O(2)-induced toxicity, and that an antioxidant mechanism through ROS scavenging may be in part responsible for cells neuroprotection.
authors.affiliation: Faculty of Health Sciences, San Jorge University, Villanueva de Gállego, Zaragoza, Spain. ilopez@usj.es
authors.forename: Víctor
authors.initials: V
authors.lastname: López
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Calvo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D028241:Camellia sinensis / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010851:Picrates; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D018696:Neuroprotective Agents; D010851:Picrates; D017382:Reactive Oxygen Species; D013662:Tea; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl