title: A human model of selenium that integrates metabolism from selenite and selenomethionine.
abstract: Selenium (Se) metabolism is affected by its chemical form in foods and by its incorporation (specific vs. nonspecific) into multiple proteins. Modeling Se kinetics may clarify the impact of form on metabolism. Although the kinetics of Se forms have been compared in different participants, or the same participants at different times, direct comparisons of their respective metabolism in the same participants have not been made. The aim of this study was to simultaneously compare kinetics of absorbed Se from inorganic selenite (Sel) and organic selenomethionine (SeMet) in healthy participants (n = 31). After oral administration of stable isotopic tracers of each form, urine and feces were collected for 12 d and blood was sampled over 4 mo. Tracer enrichment was determined by isotope-dilution-GC-MS. Using WinSAAM, a compartmental model was fitted to the data. Within 30 min of ingestion, Se from both forms entered a common pool, and metabolism was similar for several days before diverging. Slowly turning-over pools were required in tissues and plasma for Se derived from SeMet to account for its 3-times-higher incorporation into RBC compared with Se from Sel; these presumably represent nonspecific incorporation of SeMet into proteins. Pool sizes and transport rates were determined and compared by form and gender. The final model consisted of 11 plasma pools, 2 pools and a delay in RBC, and extravascular pools for recycling of Se back into plasma. This model will be used to evaluate changes in Se metabolism following long-term (2 y) Se supplementation.
authors.affiliation: Metabolic Modeling Services Ltd., 7201 Blenheim, New Zealand.
authors.forename: Meryl E
authors.initials: ME
authors.lastname: Wastney
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Combs
authors.forename: Wesley K
authors.initials: WK
authors.lastname: Canfield
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Kristine Y
authors.initials: KY
authors.lastname: Patterson
authors.forename: A David
authors.initials: AD
authors.lastname: Hill
authors.forename: James E
authors.initials: JE
authors.lastname: Moler
authors.forename: Blossom H
authors.initials: BH
authors.lastname: Patterson
mesh_terms: D000328:Adult; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D012645:Selenomethionine / Q000493:pharmacokinetics*; D018038:Sodium Selenite / Q000493:pharmacokinetics*
chemical_list: D012645:Selenomethionine; D018038:Sodium Selenite