title: Differential expression of rubisco in sporophytes and gametophytes of some marine macroalgae.
abstract: Rubisco (ribulose-1, 5-bisphosphate carboxylase/oxygenase), a key enzyme of photosynthetic CO(2) fixation, is one of the most abundant proteins in both higher plants and algae. In this study, the differential expression of Rubisco in sporophytes and gametophytes of four seaweed species--Porphyra yezoensis, P. haitanensis, Bangia fuscopurpurea (Rhodophyte) and Laminaria japonica (Phaeophyceae)--was studied in terms of the levels of transcription, translation and enzyme activity. Results indicated that both the Rubisco content and the initial carboxylase activity were notably higher in algal gametophytes than in the sporophytes, which suggested that the Rubisco content and the initial carboxylase activity were related to the ploidy of the generations of the four algal species.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Fan
authors.forename: Guangce
authors.initials: G
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Niu
authors.forename: Baicheng
authors.initials: B
authors.lastname: Zhou
mesh_terms: D019735:Carbon-Carbon Ligases / Q000378:metabolism; D019755:Carbon-Carbon Lyases / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D015870:Gene Expression; D055993:Germ Cells, Plant / Q000737:chemistry / Q000378:metabolism*; D014176:Protein Biosynthesis; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis*; D012624:Seaweed / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D013170:Spores / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004798:Enzymes; D019755:Carbon-Carbon Lyases; D012273:Ribulose-Bisphosphate Carboxylase; D019735:Carbon-Carbon Ligases