title: Effect of oral supplementation of Lactobacillus reuteri in reduction of intestinal absorption of aflatoxin B(1) in rats.
abstract: The goals of this work were to assess the ability of Lactobacillus reuteri to bind aflatoxin B(1) in the intestinal tract and determine its effect on intestinal absorption of the toxin dispensed in either single or multiple doses in a murine model. Male Wistar rats were used, and two experiments were conducted after bacteria were implanted. Experiment one involved a single-oral dose of toxin, and the subsequent flow cytometric analysis of bacteria isolated from the small intestine and treated with specific FITC-labeled AFB(1) antibodies. The second experiment was carried out supplying the toxin in 7 oral sub-doses, and the later quantification of AFB(1)-Lys adducts in blood samples by ELISA assay. The results demonstrated that L. reuteri was able to bind AFB(1) in the intestinal tract, mostly in the duodenum. Furthermore, the AFB(1)-Lys adducts were present at significantly lower levels in those animals receiving AFB(1) plus bacteria than in those receiving only AFB(1). Our findings confirm that probiotic bacteria could act as biological barriers in normal intestinal conditions thereby reducing the bioavailability of AFB(1) ingested orally in a single or multiple doses, thus avoiding its toxic effects.
authors.affiliation: UNIDA-Instituto Tecnológico de Veracruz, M.A. de Quevedo, Col. Formando Hogar, Veracruz, Ver., México.
authors.forename: Adrián
authors.initials: A
authors.lastname: Hernandez-Mendoza
authors.forename: Aarón Fernando
authors.initials: AF
authors.lastname: González-Córdova
authors.forename: Belinda
authors.initials: B
authors.lastname: Vallejo-Cordoba
authors.forename: Hugo Sergio
authors.initials: HS
authors.lastname: Garcia
mesh_terms: D000284:Administration, Oral; D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D001774:Blood Chemical Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000737:chemistry; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D011041:Poisoning / Q000517:prevention & control*; D019936:Probiotics / Q000008:administration & dosage*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016604:Aflatoxin B1