title: Metabotyping of Caenorhabditis elegans and their culture media revealed unique metabolic phenotypes associated to amino acid deficiency and insulin-like signaling.
abstract: Insulin/IGF-like signaling (IIS) and nutrient sensing are among the most potent regulators of health status and aging. Here, a global view of the metabolic changes in C. elegans with impaired function of IIS represented by daf-2 and daf-16 and the intestinal di- and tripeptide transport pept-1 was generated using (1)H nuclear magnetic resonance spectroscopic analysis of worm extracts and spent culture media. We showed that specific metabolic profiles were significantly associated with each type of mutant. On the basis of the metabonomics data, selected underlying processes were further investigated using proteomic and transcriptomic approaches. The observed changes suggest a decreased activity of the one carbon metabolism in pept-1(lg601) mutants. Higher concentration of branched-chain amino acids (BCAA) and altered transcript levels of genes involved in BCAA metabolism were observed in long-living strains daf-2(e1370) and daf-2(e1370);pept-1(lg601) when compared to wild types and daf-16(m26);daf-2(e1370);pept-1(lg601) C. elegans, suggesting a DAF-16-dependent mechanism.
authors.affiliation: Nestlé Research Center, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland.
authors.forename: Francois-Pierre J
authors.initials: FP
authors.lastname: Martin
authors.forename: Britta
authors.initials: B
authors.lastname: Spanier
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Collino
authors.forename: Ivan
authors.initials: I
authors.lastname: Montoliu
authors.forename: Carolin
authors.initials: C
authors.lastname: Kolmeder
authors.forename: Pieter
authors.initials: P
authors.lastname: Giesbertz
authors.forename: Michael
authors.initials: M
authors.lastname: Affolter
authors.forename: Martin
authors.initials: M
authors.lastname: Kussmann
authors.forename: Hannelore
authors.initials: H
authors.lastname: Daniel
authors.forename: Sunil
authors.initials: S
authors.lastname: Kochhar
authors.forename: Serge
authors.initials: S
authors.lastname: Rezzi
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D004734:Energy Metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D008136:Longevity / Q000502:physiology; D055432:Metabolomics / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D010641:Phenotype; D025341:Principal Component Analysis; D015398:Signal Transduction
chemical_list: D000596:Amino Acids; D029742:Caenorhabditis elegans Proteins; D003470:Culture Media; D007328:Insulin