title: Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent manner.
abstract: BACKGROUND
  Cruciferous vegetable intake is inversely associated with the risk of several cancers. Isothiocyanates (ITC) are hypothesized to be the major bioactive constituents contributing to these cancer-preventive effects. The polymorphic glutathione-S-transferase (GST) gene family encodes several enzymes which catalyze ITC degradation in vivo.
  
  
  METHODS
  We utilized high throughput proteomics methods to examine how human serum peptides (the "peptidome") change in response to cruciferous vegetable feeding in individuals of different GSTM1 genotypes. In two randomized, crossover, controlled feeding studies (EAT and 2EAT) participants consumed a fruit- and vegetable-free basal diet and the basal diet supplemented with cruciferous vegetables. Serum samples collected at the end of the feeding period were fractionated and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry spectra were obtained. Peak identification/alignment computer algorithms and mixed effects models were used to analyze the data.
  
  
  RESULTS
  After analysis of spectra from EAT participants, 24 distinct peaks showed statistically significant differences associated with cruciferous vegetable intake. Twenty of these peaks were driven by their GSTM1 genotype (i.e., GSTM1+ or GSTM1- null). When data from EAT and 2EAT participants were compared by joint processing of spectra to align a common set, 6 peaks showed consistent changes in both studies in a genotype-dependent manner. The peaks at 6700 m/z and 9565 m/z were identified as an isoform of transthyretin (TTR) and a fragment of zinc α2-glycoprotein (ZAG), respectively.
  
  
  CONCLUSIONS
  Cruciferous vegetable intake in GSTM1+ individuals led to changes in circulating levels of several peptides/proteins, including TTR and a fragment of ZAG. TTR is a known marker of nutritional status and ZAG is an adipokine that plays a role in lipid mobilization. The results of this study present evidence that the GSTM1-genotype modulates the physiological response to cruciferous vegetable intake.
authors.affiliation: Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA.
authors.forename: Heather Ann
authors.initials: HA
authors.lastname: Brauer
authors.forename: Tanya E
authors.initials: TE
authors.lastname: Libby
authors.forename: Breeana L
authors.initials: BL
authors.lastname: Mitchell
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Chu
authors.initials: C
authors.lastname: Chen
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Randolph
authors.forename: Yutaka Y
authors.initials: YY
authors.lastname: Yasui
authors.forename: Johanna W
authors.initials: JW
authors.lastname: Lampe
authors.forename: Paul D
authors.initials: PD
authors.lastname: Lampe
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D019607:Brassicaceae / Q000737:chemistry*; D018592:Cross-Over Studies; D004032:Diet; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D017879:Isothiocyanates / Q000008:administration & dosage; D016014:Linear Models; D008297:Male; D010455:Peptides / Q000097:blood*; D011228:Prealbumin / Q000032:analysis; D012307:Risk Factors; D014675:Vegetables / Q000737:chemistry*; D055815:Young Adult; D060749:alpha-2-HS-Glycoprotein
chemical_list: D001798:Blood Proteins; D005982:Glutathione Transferase; D017879:Isothiocyanates; D010455:Peptides; D011228:Prealbumin; D060749:alpha-2-HS-Glycoprotein; C556354:AHSG protein, human; C037152:isothiocyanic acid