title: Moderate prenatal alcohol exposure and serotonin genotype interact to alter CNS serotonin function in rhesus monkey offspring.
abstract: BACKGROUND
  Moderate prenatal alcohol exposure can contribute to neurodevelopmental impairments and disrupt several neurotransmitter systems. We examined the timing of moderate level alcohol exposure, serotonin transporter gene polymorphic region variation (rh5-HTTLPR), and levels of primary serotonin and dopamine (DA) metabolites in cerebrospinal fluid (CSF) in rhesus monkeys.
  
  
  METHODS
  Thirty-two 30-month old rhesus monkeys (Macaca mulatta) from 4 groups of females were assessed: (i) early alcohol-exposed group (n = 9), in which mothers voluntarily consumed 0.6 g/kg/d alcohol solution on gestational days 0 to 50; (ii) middle-to-late gestation alcohol-exposed group (n = 6), mothers consumed 0.6 g/kg/d alcohol solution on gestational days 50 to 135; (iii) a continuous-exposure group (n = 8), mothers consumed 0.6 g/kg/d alcohol solution on gestational days 0 to 135; and (iv) controls (n = 9), mothers consumed an isocaloric control solution on gestational days 0 to 50, 50 to 135, or 0 to 135. Serotonin transporter promoter region allelic variants (homozygous s/s or heterozygous s/l vs. homozygous l/l) were determined. We examined CSF concentrations of the 5-HT and DA metabolites, 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA), respectively, at baseline and 50 hours after separation from cage-mates, when the monkeys were 30 months old.
  
  
  RESULTS
  Early- and middle-to-late gestation-alcohol exposed monkeys carrying the short allele had lower concentrations of 5-HIAA in CSF relative to other groups. Concentrations of 5-HIAA in CSF were lower for s allele carriers and increased from baseline relative to pre-separation values, whereas 5-HIAA levels in l/l allele carriers were not affected by separation. Monkeys carrying the short allele had lower basal concentrations of HVA in CSF compared with monkeys homozygous for the long allele.
  
  
  CONCLUSIONS
  Carrying the s allele of the 5-HT transporter increased the probability of reduced 5-HIAA in early- and middle-to-late gestation alcohol-exposed monkeys and reduced HVA at baseline. These findings that prenatal alcohol exposure altered central 5-HT activity in genetically sensitive monkeys raise questions about whether abnormal serotonin biological pathways could underlie some of the psychiatric disorders reported in fetal alcohol spectrum disorder.
authors.affiliation: Department of Kinesiology, University of Wisconsin-Madison, 53706, USA. schneider@education.wisc.edu
authors.forename: Mary L
authors.initials: ML
authors.lastname: Schneider
authors.forename: Colleen F
authors.initials: CF
authors.lastname: Moore
authors.forename: Christina S
authors.initials: CS
authors.lastname: Barr
authors.forename: Julie A
authors.initials: JA
authors.lastname: Larson
authors.forename: Gary W
authors.initials: GW
authors.lastname: Kraemer
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects / Q000502:physiology*; D000431:Ethanol / Q000008:administration & dosage*; D005260:Female; D005838:Genotype; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid; D008253:Macaca mulatta; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000134:cerebrospinal fluid / Q000235:genetics*; D011897:Random Allocation; D012701:Serotonin / Q000134:cerebrospinal fluid / Q000235:genetics*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000134:cerebrospinal fluid / Q000235:genetics*
chemical_list: D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin; D000431:Ethanol; D006897:Hydroxyindoleacetic Acid