title: Endoplasmic reticulum dynamics in hippocampal dendritic spines induced by agonists of type I metabotropic glutamate but not by muscarinic acetylcholine receptors.
abstract: Neurons in the hippocampus exhibit subpopulations of dendritic spines that contain endoplasmic reticulum (ER). ER in spines is important for synaptic activity and its associated Ca(2+) signaling. The dynamic distribution of ER to spines is regulated by diacylglycerol and partly mediated by protein kinase C, metalloproteinases and γ-secretase. In this study, we explored whether pharmacological activation of type I metabotropic glutamate receptors (mGluRs) and muscarinic acetylcholine receptors (mAChRs) known to activate phospholipase C would have any effect on spine ER content. We found that DHPG (100 μM) but not carbachol (10 μM) caused a reduction in the number of spines with ER. We further found that ER Ca(2+) depletion triggered by thapsigargin (200 nM) had no effect on ER localization in spines.
authors.affiliation: Department of Clinical Sciences Lund, Laboratory for Experimental Brain Research, Wallenberg Neuroscience Centre, Lund University, BMC A13, 221 84 Lund, Sweden. Ai_Na.Ng@med.lu.se
authors.forename: Ai Na
authors.initials: AN
authors.lastname: Ng
authors.forename: Håkan
authors.initials: H
authors.lastname: Toresson
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000187:drug effects / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D056004:Molecular Dynamics Simulation; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists* / Q000502:physiology*; D011976:Receptors, Muscarinic / Q000502:physiology*
chemical_list: D018094:Receptors, Metabotropic Glutamate; D011976:Receptors, Muscarinic; C104077:metabotropic glutamate receptor type 1; D008734:Methoxyhydroxyphenylglycol; D002217:Carbachol; C010117:3,4-dihydroxyphenylglycol