title: Identification of genes essential for prey-independent growth of Bdellovibrio bacteriovorus HD100.
abstract: Bdellovibrio bacteriovorus HD100 is an obligate predatory bacterium that attacks and invades Gram-negative bacteria. The predator requires living bacteria to survive as growth and replication take place inside the bacterial prey. It is possible to isolate mutants that grow and replicate outside prey bacteria. Such mutants are designated host or prey independent, and their nutritional requirements vary. Some mutants are saprophytic and require prey extracts for extracellular growth, whereas other mutants grow axenically, which denotes the formation of colonies on complete medium in the absence of any prey components. The initial events leading to prey-independent growth are still under debate, and several genes may be involved. We selected new mutants by three different methods: spontaneous mutation, transposon mutagenesis, and targeted gene knockout. By all approaches we isolated mutants of the hit (host interaction) locus. As the relevance of this locus for the development of prey independence has been questioned, we performed whole-genome sequencing of five prey-independent mutants. Three mutants were saprophytic, and two mutants could grow axenically. Whole-genome analysis revealed that the mutation of a small open reading frame of the hit locus is sufficient for the conversion from predatory to saprophytic growth. Complementation experiments were performed by introduction of a plasmid carrying the wild-type hit gene into saprophytic mutants, and predatory growth could be restored. Whole-genome sequencing of two axenic mutants demonstrated that in addition to the hit mutation the colony formation on complete medium was shown to be influenced by the mutations of two genes involved in RNA processing. Complementation experiments with a wild-type gene encoding an RNA helicase, RhlB, abolished the ability to form colonies on complete medium, indicating that stability of RNA influences axenic growth.
authors.affiliation: Bundesinstitut für Risikobewertung (BfR), Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Roschanski
authors.forename: Sven
authors.initials: S
authors.lastname: Klages
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Michael
authors.initials: M
authors.lastname: Linscheid
authors.forename: Eckhard
authors.initials: E
authors.lastname: Strauch
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001501:Bdellovibrio / Q000502:physiology*; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006090:Gram-Negative Bacteria; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media