title: The solute carrier family 15A4 regulates TLR9 and NOD1 functions in the innate immune system and promotes colitis in mice.
abstract: OBJECTIVE
  Solute carrier family 15 (SLC15) A4 is a proton-coupled histidine and oligopeptide cotransporter expressed by the immune and nervous systems and associated with disorders such as inflammatory bowel diseases and systemic lupus erythematosus. High levels of SLC15A4 transcripts were observed in human antigen-presenting cells, including dendritic cells, activated macrophages, and B cells. However, the roles of SLC15A4 in the immune regulation are not known. We investigated the function of SLC15A4 in the innate immune system.
  
  
  METHODS
  We created SLC15A4-deficient (SLC15A4(-/-)) mice and compared Toll-like receptor 9 and NOD1-dependent innate immune responses between SLC15A4(-/-) and control (SLC15A4(+/+)) mice.
  
  
  RESULTS
  SLC15A4 deficiency impaired CpG-induced production of interleukin-12, interleukin-15, and interleukin-18 by dendritic cells. Correspondingly, SLC15A4(-/-) mice developed a less severe form of Th1-dependent colitis than SLC15A4(+/+) mice. Increased lysosomal histidine, in the absence of SLC15A4, appears to negatively regulate Toll-like receptor 9 function by inhibiting the proteolytic activities of cathepsins B and L. SLC15A4(-/-) mice also had a severe defect in NOD1-dependent cytokine production, indicating that SLC15A4 functions as a transporter of the NOD1 ligand.
  
  
  CONCLUSIONS
  SLC15A4 promotes colitis through Toll-like receptor 9 and NOD1-dependent innate immune responses. Histidine homeostasis within intracellular compartments is important for eliciting effective innate immune responses.
authors.affiliation: Department of Gastroenterology, Research Institute, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Sasawatari
authors.forename: Tadashi
authors.initials: T
authors.lastname: Okamura
authors.forename: Eiji
authors.initials: E
authors.lastname: Kasumi
authors.forename: Kaori
authors.initials: K
authors.lastname: Tanaka-Furuyama
authors.forename: Rieko
authors.initials: R
authors.lastname: Yanobu-Takanashi
authors.forename: Senji
authors.initials: S
authors.lastname: Shirasawa
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kato
authors.forename: Noriko
authors.initials: N
authors.lastname: Toyama-Sorimachi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003092:Colitis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D004247:DNA / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007150:Immunohistochemistry; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D053474:Nod1 Signaling Adaptor Protein / Q000276:immunology* / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000276:immunology* / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D053474:Nod1 Signaling Adaptor Protein; C507873:Nod1 protein, mouse; C554281:Slc15a4 protein, mouse; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D004247:DNA