title: Sodium sulfite aggravated allergic sensitization and airway inflammation in mite allergen sensitized BALB/c mice.
abstract: Sulfur dioxide is a typical air pollutant. Sulfite, which is formed at the bronchial mucosa from inhaled sulfur dioxide, might play a role in the exacerbation of asthma. In this study, we investigated the effects of sodium sulfite and its interaction with a house dust mite (Dermatophagoides pteronyssinus, Der p) on allergic sensitization and airway inflammation. BALB/c mice were divided into four groups: control (n = 10), mite intranasal (mIN, n = 12), sodium sulfite intranasal (sIN, n = 12) and mIN + sIN (n = 12). In non-control groups, the mice were sensitized on day 8 and day 15 with mite allergen subcutaneously. Mite allergen was then administrated intranasally from day 15 to day 22 in mIN and mIN+sIN groups. Sodium sulfite was administrated in sIN and mIN + sIN groups intranasally from day 1 to day 22. Plasma Der p-specific IgE, IgG2a, lung histopathology and cytokine levels (IL-5 and IFN-γ) were analyzed. In comparison between mIN (or sIN) and mIN + sIN group, Der p-specific IgE levels were significantly higher in mIN + sIN group (p < 0.01). Besides, Der p-specific IgG2a level was significantly lower in mIN + sIN group than mIN (or sIN) group (p < 0.01). The peribronchiolar, alveolar and total inflammatory scores were increased in the mIN + sIN group comparing with the control group (p < 0.05, p < 0.01, p < 0.01, respectively). Lung supernatant in mIN + sIN group has higher IL-5/IFN-γ ratio than control, mIN or sIN group (all p < 0.05). Our study concluded sodium sulfite may enhance allergic sensitization as well as airway inflammation in mite allergen sensitized BALB/c mice.
authors.affiliation: 1Division of Immunology and Nephrology, Department of Pediatrics, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Heng-Kuei
authors.initials: HK
authors.lastname: Lin
authors.forename: Jaw-Ji
authors.initials: JJ
authors.lastname: Tsai
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Wen
authors.forename: Ming-Chin
authors.initials: MC
authors.lastname: Tsai
authors.forename: Ciao-Jin
authors.initials: CJ
authors.lastname: Chen
authors.forename: Lin-Shien
authors.initials: LS
authors.lastname: Fu
mesh_terms: D000281:Administration, Intranasal; D000393:Air Pollutants / Q000633:toxicity; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D006967:Hypersensitivity / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011014:Pneumonia / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D013447:Sulfites / Q000633:toxicity*; D013458:Sulfur Dioxide / Q000633:toxicity
chemical_list: D000393:Air Pollutants; D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D015848:Interleukin-5; D013447:Sulfites; D013458:Sulfur Dioxide; D007073:Immunoglobulin E; D007371:Interferon-gamma; C025026:sodium sulfite