title: Distinct expression of the soluble and the membrane-bound forms of interleukin-1 receptor accessory protein in the endometrium of women with endometriosis.
abstract: OBJECTIVE
  To investigate interleukin (IL) 1 receptor accessory protein (IL1RAcP) expression in the eutopic endometrium of women with endometriosis.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Human reproduction research laboratory.
  
  
  METHODS
  Sixty-six women with endometriosis and 60 healthy women with no laparoscopic evidence of endometriosis.
  
  
  METHODS
  Endometrial tissue samples were obtained during laparoscopic surgery.
  
  
  METHODS
  IL1RAcP protein expression was analyzed by immunohistochemistry, Western blot, and ELISA, and IL1RAcP mRNA expression was analyzed by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  This study showed a significant downregulation of soluble (s)IL1RAcP expression in the eutopic endometrium of women with endometriosis compared to normal women, occurring at the protein or mRNA level. This finding appeared to vary according to endometriosis stage, being more obvious in the earliest (I and II) than the latest (III and IV) stages of the disease. However, the membrane-bound IL1RAcP did not show any noticeable endometriosis-related change, neither at the protein or mRNA level.
  
  
  CONCLUSIONS
  In view of the wide spectrum of IL-1 inflammatory and growth-promoting effects, downregulation of sIL1RAcP, which is known for inhibiting IL1, indicates a profound defect in the capability of endometrial cells of women with endometriosis to counterregulate IL-1 effects and may represent an important mechanism underlying the ability of these cells to implant and develop into host tissues.
authors.affiliation: Faculty of Medicine, Reproductive Endocrinology, Research Centre, Saint-Francis of Assisi Hospital, CHUQ, Laval University, Quebec, Canada.
authors.forename: Sophie
authors.initials: S
authors.lastname: Guay
authors.forename: Nadège
authors.initials: N
authors.lastname: Michaud
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bourcier
authors.forename: Mathieu
authors.initials: M
authors.lastname: Leboeuf
authors.forename: Madeleine
authors.initials: M
authors.lastname: Lemyre
authors.forename: Jacques
authors.initials: J
authors.lastname: Mailloux
authors.forename: Ali
authors.initials: A
authors.lastname: Akoum
mesh_terms: D000328:Adult; D002462:Cell Membrane / Q000378:metabolism*; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D053577:Interleukin-1 Receptor Accessory Protein / Q000096:biosynthesis* / Q000235:genetics; D040081:Protein Array Analysis; D012189:Retrospective Studies; D012995:Solubility; D055815:Young Adult
chemical_list: D053577:Interleukin-1 Receptor Accessory Protein