title: Diversity and dynamics of bacterial populations during spontaneous sorghum fermentations used to produce ting, a South African food.
abstract: Ting is a spontaneously fermented sorghum food that is popular for its sour taste and unique flavour. Insight of the microbial diversity and population dynamics during sorghum fermentations is an essential component of the development of starter cultures for commercial production of ting. In this study, bacterial populations associated with spontaneous sorghum fermentations were examined using a culture-independent strategy based on denaturing gradient gel electrophoresis and sequence analysis of V3-16S rRNA gene amplicons, and a culture-dependent strategy using conventional isolation based on culturing followed by 16S rRNA and/or pheS gene sequence analysis. The entire fermentation process was monitored over a 54 h period and two phases were observed with respect to pH evolution and microbial succession. The first phase of the process (0-6h) was characterized by relatively high pH conditions and the presence of Enterococcus mundtii, albeit that this species was only detected with the culture-dependent approach. The second phase of the fermentation process (12-54 h) was characterized by increased acidity and the predominance of a broader range of lactic acid bacteria, including Lactococcus lactis, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus rhamnosus, Weissella cibaria, Enterococcus faecalis, and a close relative of Lactobacillus curvatus, as well as some members of the Enterobacteriaceae family. The Lb. curvatus-like species was only detected with PCR-DGGE, while the majority of the other species was only detected using the culture-dependent approach. These findings highlighted the fact that a combination of both approaches was essential in revealing the microbial diversity and dynamics during spontaneous sorghum fermentations.
authors.affiliation: Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria 0002, South Africa.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Madoroba
authors.forename: Emma T
authors.initials: ET
authors.lastname: Steenkamp
authors.forename: Jacques
authors.initials: J
authors.lastname: Theron
authors.forename: Ilse
authors.initials: I
authors.lastname: Scheirlinck
authors.forename: T Eugene
authors.initials: TE
authors.lastname: Cloete
authors.forename: Geert
authors.initials: G
authors.lastname: Huys
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D044822:Biodiversity; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005285:Fermentation; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D056546:Lactobacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013294:Lactococcus lactis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D045868:Sorghum / Q000378:metabolism / Q000382:microbiology*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010652:Phenylalanine-tRNA Ligase