title: Effect of curcumin and irradiation in PE/CA-PJ15 oral squamous cell carcinoma.
abstract: OBJECTIVE
  An in vitro study was made to evaluate the effect of curcumin and irradiation upon oral squamous cell carcinoma.
  
  
  METHODS
  Curcumin was administered at doses of 3, 3.75, 4.50 and 5.25 μM in PE/CA-PJ15 oral squamous cell carcinoma cultures irradiated with different doses (1, 2.5 and 5 Gy), followed by evaluation of the effects upon cell viability after 24, 48 and 72 h, based on the MTT colorimetric test.
  
  
  RESULTS
  The application of curcumin to the PECA/PJ15 tumor cells during 24, 48 and 72 h of incubation without irradiation exerted an inhibitor effect upon cell viability. The curcumin concentration at which the inhibition of cell viability proved maximum was 5.25 μM, with statistically significant differences for 24 h (p = 0.002), 48 h (p < 0.001) and 72 h of incubation (p < 0.001). In contrast, the combination of curcumin and irradiation exerted a synergic effect-the greatest effects in relation to cell viability being recorded with a curcumin concentration of 3.75 μM and 5 Gy of irradiation, in the studied cell line.
  
  
  CONCLUSIONS
  Curcumin increases cytotoxic activity in the PE/CA PJ15 cell line, while the combination of curcumin and irradiation exerts a synergic effect.
authors.affiliation: Clínica Odontológica Universitaria, Medicina Bucal, Hospital Morales Meseguer, Murcia, Spain. majornet@um.es
authors.forename: Pia
authors.initials: P
authors.lastname: López-Jornet
authors.forename: Fabio
authors.initials: F
authors.lastname: Camacho-Alonso
authors.forename: Francisco
authors.initials: F
authors.lastname: Gómez-Garcia
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003124:Colorimetry; D004396:Coloring Agents; D003474:Curcumin / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000532:radiotherapy*; D020360:Neoadjuvant Therapy; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000627:therapeutic use; D011879:Radiotherapy Dosage; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D004396:Coloring Agents; D011838:Radiation-Sensitizing Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D003474:Curcumin