title: Effects of buyang huanwu tang combined with bone marrow mesenchymal stem cell transplantation on the expression of VEGF and Ki-67 in the brain tissue of the cerebral ischemia-reperfusion model rat.
abstract: OBJECTIVE
  To explore the mechanism of Buyang Huanwu Tang (Decoction Invigorating Yang for Recuperation) combined with bone marrow mesenchymal stem cells (MSCs) transplantation in protecting nerves of cerebral ischemic injury.
  
  
  METHODS
  Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang was administrated. Vascular endothelial growth factor (VEGF) and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method.
  
  
  RESULTS
  VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group (P < 0.05), and with the most strongest effect in the combination group.
  
  
  CONCLUSIONS
  Buyang Huanwu Tang combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou 450000, China. Zhang@yahoo.com.cn
authors.forename: Yun-Ke
authors.initials: YK
authors.lastname: Zhang
authors.forename: Xue-Yong
authors.initials: XY
authors.lastname: Han
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Che
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics* / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D019394:Ki-67 Antigen; D042461:Vascular Endothelial Growth Factor A