title: Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.
abstract: The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.
authors.affiliation: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. atarashi@nagasaki-u.ac.jp
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Atarashi
authors.forename: Katsuya
authors.initials: K
authors.lastname: Satoh
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sano
authors.forename: Takayuki
authors.initials: T
authors.lastname: Fuse
authors.forename: Naohiro
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ishibashi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Matsubara
authors.forename: Takehiro
authors.initials: T
authors.lastname: Nakagaki
authors.forename: Hitoki
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Susumu
authors.initials: S
authors.lastname: Shirabe
authors.forename: Masahito
authors.initials: M
authors.lastname: Yamada
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Mizusawa
authors.forename: Tetsuyuki
authors.initials: T
authors.lastname: Kitamoto
authors.forename: Genevieve
authors.initials: G
authors.lastname: Klug
authors.forename: Amelia
authors.initials: A
authors.lastname: McGlade
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Collins
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nishida
mesh_terms: D052160:Benzothiazoles; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001921:Brain / Q000378:metabolism; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D005456:Fluorescent Dyes; D006801:Humans; D009038:Motion; D018620:PrPSc Proteins / Q000134:cerebrospinal fluid; D011328:Prions / Q000134:cerebrospinal fluid; D012680:Sensitivity and Specificity; D013844:Thiazoles
chemical_list: D052160:Benzothiazoles; D015415:Biomarkers; D005456:Fluorescent Dyes; D018620:PrPSc Proteins; D011328:Prions; D013844:Thiazoles; C009462:thioflavin T