title: Characterization of rabies virus from a human case in Nepal.
abstract: Rabies is endemic throughout most of Asia, with the majority of human cases transmitted by domestic dogs (Canis familiaris). Here, we report a case of rabies in a 12-year-old girl in the Lalitpur district of Nepal that might have been prevented by better public awareness and timely post-exposure prophylaxis. Molecular characterization of the virus showed 100% identity over a partial nucleoprotein gene sequence to previous isolates from Nepal belonging to the 'arctic-like' lineage of rabies virus. Sequence analysis of both partial nucleoprotein and glycoprotein genes showed differences in consensus sequence after passage in vitro but not after passage in vivo.
authors.affiliation: Rabies Vaccine Production Laboratory, Tripureshwor, Kathmandu, Nepal.
authors.forename: G R
authors.initials: GR
authors.lastname: Pant
authors.forename: D L
authors.initials: DL
authors.lastname: Horton
authors.forename: M
authors.initials: M
authors.lastname: Dahal
authors.forename: J N
authors.initials: JN
authors.lastname: Rai
authors.forename: S
authors.initials: S
authors.lastname: Ide
authors.forename: S
authors.initials: S
authors.lastname: Leech
authors.forename: D A
authors.initials: DA
authors.lastname: Marston
authors.forename: L M
authors.initials: LM
authors.lastname: McElhinney
authors.forename: A R
authors.initials: AR
authors.lastname: Fooks
mesh_terms: D002648:Child; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009390:Nepal; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D011818:Rabies / Q000821:virology*; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins