title: Characterization of Plasmodium vivax transmission-blocking activity in low to moderate malaria transmission settings of the Colombian Pacific coast.
abstract: Malaria infection induces antibodies capable of suppressing the infectivity of gametocytes and gametes, however, little is known about the duration of the antibody response, the parasite specificity, and the role of complement. We report the analyses of the transmission-blocking (TB) activity of sera collected from 105 Plasmodium vivax-infected and 44 non-infected individuals from a malaria endemic region of Colombia, using a membrane feeding assay in Anopheles albimanus mosquitoes. In infected donors we found that TB activity was antibody dose dependent (35%), lasted for 2-4 months after infection, and in 70% of the cases different P. vivax wild isolates displayed differential susceptibility to blocking antibodies. Additionally, in a number of assays TB was complement-dependent. Twenty-seven percent of non-infected individuals presented TB activity that correlated with antibody titers. Studies here provide preliminary data on factors of great importance for further work on the development of TB vaccines.
authors.affiliation: Instituto de Inmunología del Valle, Universidad del Valle, Cali, Colombia. marevalo@inmuno.org
authors.forename: Myriam
authors.initials: M
authors.lastname: Arévalo-Herrera
authors.forename: Yezid
authors.initials: Y
authors.lastname: Solarte
authors.forename: Leonardo
authors.initials: L
authors.lastname: Rocha
authors.forename: Diego
authors.initials: D
authors.lastname: Alvarez
authors.forename: John C
authors.initials: JC
authors.lastname: Beier
authors.forename: Sócrates
authors.initials: S
authors.lastname: Herrera
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology; D019138:Antibodies, Blocking / Q000097:blood / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology*; D003105:Colombia / Q000453:epidemiology; D003165:Complement System Proteins / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004375:Duffy Blood-Group System; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D016780:Malaria, Vivax / Q000453:epidemiology / Q000276:immunology* / Q000635:transmission*; D010966:Plasmodium vivax / Q000276:immunology*
chemical_list: D019138:Antibodies, Blocking; D000913:Antibodies, Protozoan; D004375:Duffy Blood-Group System; D007106:Immune Sera; D003165:Complement System Proteins