title: Generation of T cell hybridomas from naturally occurring FoxP3+ regulatory T cells.
abstract: Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs is an obstacle in generation of highly dividing Treg population for their fusion. In this chapter, we describe a simple and efficient method to generate large number of blasting Treg and their successful fusion with thymoma BW5147. The resultant hybridomas lose Treg-specific transcription factor FoxP3, respond to antigenic stimulation by producing IL-2, and thus allow the evaluation of antigen specific, Tregs-derived TCRs.
authors.affiliation: Medical College of Georgia, Augusta, GA, USA.
authors.forename: Nagendra
authors.initials: N
authors.lastname: Singh
authors.forename: Rafal
authors.initials: R
authors.lastname: Pacholczyk
authors.forename: Makio
authors.initials: M
authors.lastname: Iwashima
authors.forename: Leszek
authors.initials: L
authors.lastname: Ignatowicz
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006825:Hybridomas / Q000166:cytology*; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013945:Thymoma / Q000378:metabolism
chemical_list: D000941:Antigens; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse