title: Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability.
abstract: To improve the aero- and solvent tolerance of the solvent-producing Clostridium acetobutylicum, glutathione biosynthetic capability was introduced into C. acetobutylicum DSM1731 by cloning and over-expressing the gshAB genes from Escherichia coli. Strain DSM1731(pITAB) produces glutathione, and shows a significantly improved survival upon aeration and butanol challenge, as compared with the control. In addition, strain DSM1731(pITAB) exhibited an improved butanol tolerance and an increased butanol production capability, as compared with the recombinant strains with only gshA or gshB gene. These results illustrated that introducing glutathione biosynthetic pathway, which is redundant for the metabolism of C. acetobutylicum, can increase the robustness of the host to achieve a better solvent production.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
authors.forename: Linjiang
authors.initials: L
authors.lastname: Zhu
authors.forename: Hongjun
authors.initials: H
authors.lastname: Dong
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics; D005818:Genetic Engineering; D005978:Glutathione / Q000096:biosynthesis / Q000235:genetics; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D000440:Butanols; D029968:Escherichia coli Proteins; D005978:Glutathione