title: Histopathologic evaluation of patent ductus arteriosus stents after hybrid stage I palliation.
abstract: The aim of this study was to determine the histopathology of patent ductus arteriosus (PDA) in-stent stenosis after hybrid stage I palliation. The hybrid approach to palliation of hypoplastic left heart syndrome can be complicated by the development of in-stent stenosis of the PDA. This may obstruct retrograde aortic arch flow, decrease systemic circulation, and lead to interstage interventional procedures. Stented PDA samples removed from eight patients undergoing comprehensive stage II repair were examined by way of radiography and histochemistry (hematoxylin and eosin, Movat pentachrome, α-smooth muscle actin, and proliferating cell nuclear antigen). A retrospective chart review of the patients was also performed. PDA stents were in place in the PDA for a mean period of 169 ± 28 days in patients who had a mean age of 176 ± 30 days at the time of stent removal. Stent deployment caused chronic inflammation, caused fibrin deposition, and induced vascular smooth muscle-cell (VSMC) proliferation in the area immediately surrounding the stent struts. The neointimal region was composed largely of smooth muscle cells that appeared to be fully differentiated by the lack of PCNA staining. Neointimal thickening occurs in the PDA after stent placement for hybrid palliation of HLHS and is the result of inflammation, extracellular matrix deposition, and smooth muscle-cell proliferation in the peristrut region. This finding suggests that proliferating VSMCs in the peristrut region may provide the impetus for inward neointimal formation and therefore the manifestation of in-stent stenosis.
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Egan
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Trask
authors.affiliation: Department of Pathology, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Baker
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lawrence
authors.affiliation: CVPath Institute, Gaithersburg, MD, USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Ladich
authors.affiliation: CVPath Institute, Gaithersburg, MD, USA.
authors.forename: Renu
authors.initials: R
authors.lastname: Virmani
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Hill
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Cheatham
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Galantowicz
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Lucchesi
authors.affiliation: The Heart Center, Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, OH 43205, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Kovalchin
mesh_terms: D006348:Cardiac Surgical Procedures / Q000009:adverse effects* / Q000295:instrumentation; D049109:Cell Proliferation; D003251:Constriction, Pathologic; D004373:Ductus Arteriosus / Q000473:pathology* / Q000601:surgery; D004374:Ductus Arteriosus, Patent / Q000473:pathology / Q000601:surgery*; D006801:Humans; D007223:Infant; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D010166:Palliative Care / Q000379:methods*; D011475:Prosthesis Failure; D015607:Stents