title: CD34⁺ collection efficiency as a function of blood volumes processed in pediatric autologous peripheral blood stem cell collection.
abstract: OBJECTIVE
  To characterize the relationship between CD34(+) collection efficiency and blood volumes processed in pediatric patients undergoing autologous peripheral blood stem cell (PBSC) collection.
  
  
  METHODS
  Retrospective 8-year (2001-2009) study of pediatric patients (n = 79) with neuroblastoma and central nervous system (CNS) tumors undergoing first day of autologous PBSC harvest using MNC program on the COBE Spectra (Caridian BCT, Lakewood, CO) was performed. Patients undergoing 0 to 2.9 BV (standard volume), 3 to 6 BV (large volume), and greater than 6 BV (ultra large volume) harvests were evaluated for CD34(+) collection efficiency, diagnosis (neuroblastoma vs. nonneuroblastoma), disease type (primary vs. relapse), mobilization regimen, granulocyte colony stimulating factor (GCSF) dose, and apheresis complications.
  
  
  RESULTS
  CD34(+) collection efficiencies (CE) for neuroblastoma patients were 67%, 50%, and 53% for standard (n = 14), large (n = 9), and ultra large (n = 5) volume harvests, respectively. Similarly, patients with nonneuroblastoma diagnoses had CD34(+) CE of 63%, 55%, and 65% for low (n = 19), large (n = 27), and ultra large (n = 5) volume harvests, respectively. Weight, granulocyte colony stimulating factor (G-CSF) stimulation, type of mobilization, and apheresis complications (normalized by run time) were similar between the standard, large, and ultra large volume groups in patients with either neuroblastoma or nonneuroblastoma diagnoses.
  
  
  CONCLUSIONS
  CD34(+) collection efficiency in pediatric autologous PBSC collection on the first day of harvest does not decrease with higher numbers of blood volumes processed in patients with either neuroblastoma or nonneuroblastoma primary disease. These results indirectly indicate bone marrow CD34(+) cell mobilization occurs with longer apheresis procedures in pediatric patients.
authors.affiliation: Division of Bone Marrow Transplantation, Children's National Medical Center, Center for Cancer and Blood Disorders, Washington, DC 20010, USA. Ldubrovs@cnmc.org
authors.forename: Leonid
authors.initials: L
authors.lastname: Dubrovsky
authors.forename: Edward C C
authors.initials: EC
authors.lastname: Wong
authors.forename: Evelio
authors.initials: E
authors.lastname: Perez-Albuerne
authors.forename: Brett
authors.initials: B
authors.lastname: Loechelt
authors.forename: Naynesh
authors.initials: N
authors.lastname: Kamani
authors.forename: Jane
authors.initials: J
authors.lastname: Sande
authors.forename: Kathy
authors.initials: K
authors.lastname: Mintz
authors.forename: Wendy
authors.initials: W
authors.lastname: Paul
authors.forename: Naomi L C
authors.initials: NL
authors.lastname: Luban
authors.forename: Brian R
authors.initials: BR
authors.lastname: Rood
authors.forename: Terry
authors.initials: T
authors.lastname: Fry
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001769:Blood; D002675:Child, Preschool; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis / Q000379:methods / Q000592:standards*; D012189:Retrospective Studies; D014182:Transplantation, Autologous / Q000379:methods
chemical_list: D018952:Antigens, CD34