title: Isolation of high-quality RNA from various tissues of Jatropha curcas for downstream applications.
abstract: A method for isolating transcriptionally active RNA for downstream applications from diverse tissues of Jatropha curcas, a plant rich in latex, lipids, waxes, polysaccharide, polyphenols, and secondary metabolites, is described. The described method uses alkaline borate buffer during tissue homogenization to negate the formation of viscous gel observed in guanidium-salt-containing methods. By this method, quality RNA was extracted from leaf, immature inflorescence, endosperm, and root tissues with yields ranging from 1.80 to 7.80mg/100mg fresh weight (FW). The total RNA obtained was found to be suitable for poly(A)(+)RNA purification, complementary DNA (cDNA) synthesis, cloning of full-length cDNA, and cDNA library construction.
authors.affiliation: Plant Metabolic Engineering Group, Reliance Life Sciences, Rabale, Navi Mumbai 400 701, India.
authors.forename: G Raja Krishna
authors.initials: GR
authors.lastname: Kumar
authors.forename: Nalini
authors.initials: N
authors.lastname: Eswaran
authors.forename: T Sudhakar
authors.initials: TS
authors.lastname: Johnson
mesh_terms: D001881:Borates / Q000737:chemistry; D031286:Jatropha / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D018749:RNA, Plant / Q000302:isolation & purification*
chemical_list: D001881:Borates; D018749:RNA, Plant