title: A selenium-dependent glutathione peroxidase in the Japanese scallop, Mizuhopecten yessoensis: cDNA cloning, promoter sequence analysis and mRNA expression.
abstract: Glutathione peroxidase (GPx) is an antioxidant enzyme that protects cells from oxidative damage in the innate immune responses against bacterial infections. GPx is also involved in immune defenses. In this study, we report cloning and characterization of a GPx (designated as MyGPx) coding sequences and promoter from Japanese scallop, Mizuhopecten yessoensis. The full-length 1081 nt MyGPx mRNA contained a 28 nt 5' untranslated region (UTR), a 603 nt open reading frame and a 450 nt 3' UTR containing a polyadenylation signal (AATAAA). Multiple sequence alignment revealed that amino acids essential to enzymatic function of MyGPx proteins were highly conserved. A 1628 nt 5'-flanking sequence of MyGPx was identified by genome walking. Here, several potential transcription factor binding sites were detected in the putative promoter region, and nine single nucleotide polymorphisms (SNPs) were found in the 5' sequence flanking the promoter region. Quantitative Real time PCR (qRT-PCR) was employed to measure GPx mRNA expression in adult tissues and monitor mRNA expression patterns during embryonic development and following stimulation by the bacteria Vibrillo anguillarum. Collectively, the results suggest that MyGPx fulfills an important function during M. yessoensis development and may be an important immune effector in adult molluscs.
authors.affiliation: College of Life Science, Liaoning Normal University, No. 850 Huanghe Road, Shahekou District, Dalian, China.
authors.forename: Zhongguo
authors.initials: Z
authors.lastname: Shan
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.forename: Xiangbo
authors.initials: X
authors.lastname: Bao
authors.forename: Chongbo
authors.initials: C
authors.lastname: He
authors.forename: Henan
authors.initials: H
authors.lastname: Yu
authors.forename: Weidong
authors.initials: W
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Lijun
authors.initials: L
authors.lastname: Sui
authors.forename: Bao
authors.initials: B
authors.lastname: Zhu
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012643:Selenium; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D012643:Selenium