title: Development and characterization of an environmentally friendly process sequence (autohydrolysis and organosolv) for wheat straw delignification.
abstract: The present work describes the delignification of wheat straw through an environmentally friendly process resulting from sequential application of autohydrolysis and organosolv processes. Wheat straw autohydrolysis was performed at 180°C during 30 min with a liquid-solid ratio of 10 (v/w); under these conditions, a solubilization of 44% of the original xylan, with 78% of sugars as xylooligosaccharides of the sum of sugars solubilized in the autohydrolysis liquors generated by the hemicellulose fraction hydrolysis. The corresponding solid fraction enrichment with 63.7% of glucan and 7.55% of residual xylan was treated with a 40% ethanol and 0.1% NaOH aqueous solution at a liquid-solid ratio of 10 (v/w), with the best results obtained at 180°C during 20 min. The highest lignin recovery, measured by acid precipitation of the extracted lignin, was 3.25 g/100 ml. The lignin obtained by precipitation was characterized by FTIR, and the crystallinity indexes from the native cellulose, the cellulose recovered after autohydrolysis, and the cellulose obtained after applying the organosolv process were obtained by X-ray diffraction, returning values of 21.32%, 55.17%, and 53.59%, respectively. Visualization of the fibers was done for all the processing steps using scanning electron microscopy.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: Héctor A
authors.initials: HA
authors.lastname: Ruiz
authors.forename: Denise S
authors.initials: DS
authors.lastname: Ruzene
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Silva
authors.forename: Fernando F Macieira
authors.initials: FF
authors.lastname: da Silva
authors.forename: António A
authors.initials: AA
authors.lastname: Vicente
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
mesh_terms: D011232:Chemical Precipitation; D055772:Green Chemistry Technology / Q000379:methods*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D009930:Organic Chemicals / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014908:Triticum / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D009930:Organic Chemicals; D012997:Solvents; D008031:Lignin