title: [Genetic characterization of measles virus caused the measles outbreak in Xinjiang].
abstract: OBJECTIVE
  To analyze the genetic characterizations of wild type measles viruses caused the measles outbreak in Xinjiang.
  
  
  METHODS
  Vero/Slam cell were used for measles viruses isolation from the specimens collected from measles outbreaks patients. Fragment of 676 nucleotide acids of the carboxylend of nucleoprotein gene were amplified using reverse transcription-polymerase chain reaction (RT-PCR) method and then the PCR products were directly sequenced and analyzed. Phylogenetic tree was constructed based on 450 nucleotide acids of the N-terminus of nucleoprotein gene, and homological analysis was performed at nucleotide acid level.
  
  
  RESULTS
  11 measles viruses were sequenced and all belonged to H1a subgenotype. The nucleotide difference was 0-0.2% between 11 Xinjiang isolates. And the nucleotide difference was 2.2%-2.4% between Xinjiang isolates and H1 genotype reference strain.
  
  
  CONCLUSIONS
  The Measles viruses causing the measles outbreak in Xinjiang were H1a subgenotype.
authors.affiliation: People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China.
authors.forename: Mai-mai-ti
authors.initials: MM
authors.lastname: Mijiti
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Wang
authors.forename: Song-tao
authors.initials: ST
authors.lastname: Xu
authors.forename: Nai-ying
authors.initials: NY
authors.lastname: Mao
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D006801:Humans; D008457:Measles / Q000453:epidemiology* / Q000821:virology*; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus