title: Coxiella burnetii antigen-stimulated dendritic cells mediated protection against Coxiella burnetii in BALB/c mice.
abstract: Coxiella burnetii is the etiological agent of human Q fever. In this study, adaptive transfer of mouse bone marrow-derived dendritic cells (BMDCs) stimulated with C. burnetii antigen, phase I whole-cell antigen (PIAg), lipopolysaccharide (LPS)-removed PIAg (PIIAg), protein antigen Com1, or SecB significantly reduced coxiella burden in recipient mice compared with control mice. Mice that received PIIAg-pulsed BMDCs displayed substantially lower coxiella burden than recipient mice of PIAg-pulsed BMDCs after C burnetii challenge. The protection offered by the antigen-activated BMDCs was correlated with the increased proliferation of helper T (T(H)) T(H)1 CD4(+) cells, preferential development of T(H)17 cells, and impaired expansion of regulatory T lymphocytes. Our results suggest that PIIAg is far superior to PIAg in activating BMDCs to confer protection against C. burnetii in vivo, whereas Com1 and SecB are protective antigens because Com1- or SecB-pulsed BMDCs confer partial protection.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Xile
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Xiong
authors.forename: Bohai
authors.initials: B
authors.lastname: Wen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D058491:Bacterial Load; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016997:Coxiella burnetii / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011778:Q Fever / Q000276:immunology* / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial