title: High failure rate of the ViroSeq HIV-1 genotyping system for drug resistance testing in Cameroon, a country with broad HIV-1 genetic diversity.
abstract: The ViroSeq HIV-1 genotyping system is used in many African countries for drug resistance testing. In this study, we used a panel of diverse HIV-1 group M isolates circulating in Cameroon to show that the performance of this assay can be altered by the sequence variation of non-B HIV-1 strains that predominate in African settings.
authors.affiliation: Virology Laboratory CREMER/IMPM/IRD, Rue Elig-Essono, P.O. Box 1857, Yaoundé, Cameroon. avelin.aghokeng@ird.fr
authors.forename: Avelin F
authors.initials: AF
authors.lastname: Aghokeng
authors.forename: Eitel
authors.initials: E
authors.lastname: Mpoudi-Ngole
authors.forename: Julius E
authors.initials: JE
authors.lastname: Chia
authors.forename: Elvine M
authors.initials: EM
authors.lastname: Edoul
authors.forename: Eric
authors.initials: E
authors.lastname: Delaporte
authors.forename: Martine
authors.initials: M
authors.lastname: Peeters
mesh_terms: D002163:Cameroon; D024882:Drug Resistance, Viral; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA