title: [Human papillomavirus genotyping of cervical uterine preneoplastic lesions in a high risk area].
abstract: BACKGROUND
  The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted.
  
  
  OBJECTIVE
  To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC.
  
  
  METHODS
  Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped.
  
  
  RESULTS
  HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk.
  
  
  CONCLUSIONS
  HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.
authors.affiliation: Laboratorio de Patología Molecular, Departamento de Anatomía Patológica, Facultad de Medicina/Núcleo de Desarrollo Científico Tecnológico en Biorecursos, Universidad de la Frontera, Temuco, Chile.
authors.forename: Jaime
authors.initials: J
authors.lastname: López M
authors.forename: Carmen Gloria
authors.initials: CG
authors.lastname: Ili G
authors.forename: Priscilla
authors.initials: P
authors.lastname: Brebi M
authors.forename: Patricia
authors.initials: P
authors.lastname: García M
authors.forename: Italo
authors.initials: I
authors.lastname: Capurro V
authors.forename: Pablo
authors.initials: P
authors.lastname: Guzmán G
authors.forename: Eugenio
authors.initials: E
authors.lastname: Suárez P
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Ojeda F
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Roa S
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002677:Chile / Q000453:epidemiology; D005260:Female; D006801:Humans; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology / Q000821:virology*; D012720:Severity of Illness Index; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology*