title: GADD45α and annexin A1 are involved in the apoptosis of HL-60 induced by resveratrol.
abstract: Resveratrol (3,4',5-trihydroxy-trans-stilbene), one of secondary metabolites of low molecular weight present in plant, has various important biological effects. It can induce apoptosis in human leukemia cell types in vitro, although the mechanism is not fully understood. In the present study, we demonstrated reduced viability and DNA synthesis, as well as increased proportion of the subdiploid cell population, in HL-60 cells as determined by cell cycle analysis with resveratrol. Resveratrol treatment resulted in a gradual time-dependent decrease in the expression of anti-apoptotic Bcl-2 and increase in that of Bax, annexin A1, growth arrest- and DNA damage-induced gene 45α (GADD45α), and cleaved caspase-3. In addition, resveratrol markedly increased caspase-3 activity in cells. Our results suggest that resveratrol could inhibit the proliferation and induce apoptosis of HL-60 cells through a GADD45α and annexin A1/caspase-3 pathway.
authors.affiliation: Open Laboratory for Tumor Molecular Biology/Department of Biochemistry, Shantou University Medical College, Shantou, China.
authors.forename: Guanwu
authors.initials: G
authors.lastname: Li
authors.forename: Shunhua
authors.initials: S
authors.lastname: He
authors.forename: Lijun
authors.initials: L
authors.lastname: Chang
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Jenfu
authors.initials: J
authors.lastname: Chiu
mesh_terms: D017305:Annexin A1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D048708:Cell Growth Processes / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D016193:G1 Phase / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D000097642:GADD45 Proteins
chemical_list: D017305:Annexin A1; D000972:Antineoplastic Agents, Phytogenic; D053148:Caspase 3; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D000077185:Resveratrol; D013267:Stilbenes; C439070:GADD45A protein, human; D000097642:GADD45 Proteins