title: Progesterone induces a switch in oligosaccharyltransferase isoform expression: consequences on IgG N-glycosylation.
abstract: The presence of additional N-glycans in the Fab region of IgG has shown to dramatically modify the properties and functionality of these molecules including changes in antibody affinity and stability. However, the underlying molecular mechanism responsible for the presence or absence of these glycans remains unknown. Polypeptide N-linked glycosylation is catalyzed in the lumen of the endoplasmic reticulum by the oligosaccharyltransferase complex. Mammalian cells can express two isoforms of the oligosaccharyltransferase catalytic subunit (STT3-A and STT3-B), which are endowed with distinct enzymatic properties. In this work we employed a murine hybridoma cell culture to study whether the expression of STT3 isoforms could be modulated by progesterone, thus altering the pattern of IgG N-glycosylation. We found that progesterone induces a switch of STT3 isoform expression, increasing IgG N-glycosylation. These effects were dependent on the progesterone-induced blocking factor (PIBF), whose concentration was modulated by progesterone. PIBF was previously found to be an immunomodulatory molecule relevant for the maintenance of pregnancy. We concluded that the STT3-B/STT3-A ratio modulates the N-glycosylation level of IgG, in agreement with previous data showing that full N-glycosylation of polypeptides requires cooperation between both catalytic isoforms. This work provides the first evidence that STT3 isoforms can be hormonally modulated, with marked consequences on IgG N-glycosylation.
authors.affiliation: GlycoInmuno Biology Lab, Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini" (ININCA), CONICET-Universidad de Buenos Aires, Argentina.
authors.forename: M Belén
authors.initials: MB
authors.lastname: Prados
authors.forename: Julia
authors.initials: J
authors.lastname: La Blunda
authors.forename: Julia
authors.initials: J
authors.lastname: Szekeres-Bartho
authors.forename: Julio
authors.initials: J
authors.lastname: Caramelo
authors.forename: Silvia
authors.initials: S
authors.lastname: Miranda
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D004140:Dinitrophenols / Q000276:immunology; D005786:Gene Expression Regulation / Q000276:immunology; D006031:Glycosylation / Q000187:drug effects; D006602:Hexosyltransferases / Q000378:metabolism*; D006825:Hybridomas; D007074:Immunoglobulin G / Q000378:metabolism*; D056747:Immunomodulation; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D011257:Pregnancy Proteins / Q000276:immunology / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D020033:Protein Isoforms / Q000378:metabolism*
chemical_list: D019138:Antibodies, Blocking; D004140:Dinitrophenols; D007074:Immunoglobulin G; D008565:Membrane Proteins; C491677:Pibf1 protein, mouse; D011257:Pregnancy Proteins; D020033:Protein Isoforms; D011374:Progesterone; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase