title: A sensitive and selective resonance light scattering bioassay for homocysteine in biological fluids based on target-involved assembly of polyethyleneimine-capped Ag-nanoclusters.
abstract: A specific resonance light scattering bioassay for homocysteine is developed on the basis of target-involved assembly of polyethyleneimine-capped Ag-nanoclusters. The bioassay permits discriminating homocysteine from cysteine, glutathione and other amino acids, and allows sensitive and selective detection of homocysteine with a detection limit of 42 nM.
authors.affiliation: Research Center for Analytical Sciences, and Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Shao-Kai
authors.initials: SK
authors.lastname: Sun
authors.forename: He-Fang
authors.initials: HF
authors.lastname: Wang
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008027:Light; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000737:chemistry*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
chemical_list: D006710:Homocysteine; D012834:Silver; D011094:Polyethyleneimine