title: Significance of mutation in the D-loop region in cervical cancer.
abstract: OBJECTIVE
  To investigate the mutation in the D-loop region of mitochondrial DNA in cervical cancer and its influence on the changes of reactive oxygen species (ROS) and cell cycle.
  
  
  METHODS
  The D-loop region was amplified by PCR and sequenced. Reactive oxygen species and cell cycle were detected by flow cytometry in 40 specimens from cervical cancer and adjacent normal tissues. According to the sequence results, gastric cancer tissue was divided into mutation group and control group. Reactive oxygen species, apoptosis and proliferation in the two groups were compared.
  
  
  RESULTS
  Among the 40 cervical cancer specimens, 21 mutations were identified in 12 patients, the mutation rate being 30%. There were four microsatellite instabilities in the mutations. No mutation was found in the adjacent tissues. Reactive oxygen species, apoptosis and proliferation in the mutation group were all significantly higher than those in the control group.
  
  
  CONCLUSIONS
  Mutation in the D-loop region plays a role in the genesis and development of cervical cancer.
authors.affiliation: Department of Gynaecologic Oncology, Jiangsu Cancer Hospital and Research Institute, Nanjing 210009, Jiangsu Province, China. njzyb72@sina.com
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D017209:Apoptosis / Q000502:physiology; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053842:Microsatellite Instability; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics*; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species