title: The effect of solvent on the catalytic properties of microperoxidase-11.
abstract: The effect of a range of solvents on the catalytic oxidation of methyl phenyl sulfide to methyl phenyl sulfoxide by MP-11 and by a cyclodextrin derivative of MP-11 was examined. The addition of low concentrations of alcohols enhanced the initial rate of sulfoxidation, most likely due to dispersion of MP-11 aggregates. Higher alcohol concentrations resulted in a decrease in activity arising from solvation of the hydrophobic sulfide, disrupting binding to the catalyst. In alcohols, the yield of product was decreased due to increased rates of MP-11 deactivation via the formation of aldehydes (for primary alcohols) or by peroxide-based deactivation. The catalytic activity of the cyclodextrin modified MP-11 was similar to that of MP-11 itself, demonstrating that it is the N-terminal side of MP-11 which is the determinant of catalytic activity.
authors.affiliation: Materials and Surface Science Institute, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland. noreilly@wit.ie
authors.forename: Niall J
authors.initials: NJ
authors.lastname: O'Reilly
authors.forename: Edmond
authors.initials: E
authors.lastname: Magner
mesh_terms: D000818:Animals; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006736:Horses; D015394:Molecular Structure; D009206:Myocardium / Q000201:enzymology; D010544:Peroxidases / Q000737:chemistry*; D012997:Solvents
chemical_list: D012997:Solvents; D010544:Peroxidases; C021799:microperoxidase