title: Expression, crystallization and preliminary X-ray analysis of the phosphoribosylglycinamide formyltransferase from Streptococcus mutans.
abstract: Phosphoribosylglycinamide formyltransferase (PurN) from Streptococcus mutans was recombinantly expressed in Escherichia coli. An effective purification protocol was established. The purified protein, which had a purity of >95%, was identified by SDS-PAGE and MALDI-TOF MS. The protein was crystallized using the vapour-diffusion method in hanging-drop mode with PEG 3350 as the primary precipitant. X-ray diffraction data were collected to 2.1 Å resolution. Preliminary X-ray analysis indicated that the crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.25, b = 63.29, c = 131.81 Å.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Fangli
authors.initials: F
authors.lastname: Zhai
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Ruan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Liu
authors.forename: Yulin
authors.initials: Y
authors.lastname: Hu
authors.forename: Shentao
authors.initials: S
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002021:Buffers; D003460:Crystallization; D004058:Diffusion; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051026:Phosphoribosylglycinamide Formyltransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013295:Streptococcus mutans / Q000201:enzymology*; D014961:X-Ray Diffraction; D014965:X-Rays
chemical_list: D001426:Bacterial Proteins; D002021:Buffers; D011994:Recombinant Proteins; D051026:Phosphoribosylglycinamide Formyltransferase