title: Formulation and pharmacokinetic evaluation of tetracycline-loaded solid lipid nanoparticles for subcutaneous injection in mice.
abstract: The aim of this work was to prepare tetracycline-loaded solid lipid nanoparticles (Tet-SLN), and to evaluate the potential of these colloidal carriers for subcutaneous injection. Tet-SLN was prepared by microemulsion method and the preparation conditions were optimized by ternary phase diagram. At optimized process conditions, lyophilized Tet-SLN showed spherical particles with a mean diameter of 87.2±46.9 nm and a negative zeta potential of -6.69 mV, up to 1.7% tetracycline drug content was achieved after loading. In vitro release test showed a biphasic release profile for Tet-SLN and more than 80% of the drug was liberated from Tet-SLN in 48 h. After subcutaneous injection of Tet-SLN to mice, a considerable sustained release was observed; tetracycline in blood could be detected lasting 36 h, and lower concentrations of tetracycline in all tissues tested compared to the free tetracycline solution were observed. In conclusion, Tet-SLN can be prepared well by microemulsion method and subcutaneous injection of SLN provide a new perspective for drug sustained release.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Zhenjiang, PR China. xmxu@ujs.edu.cn
authors.forename: Xi-ming
authors.initials: XM
authors.lastname: Xu
authors.forename: Yan-song
authors.initials: YS
authors.lastname: Wang
authors.forename: Rong-ying
authors.initials: RY
authors.lastname: Chen
authors.forename: Chun-lai
authors.initials: CL
authors.lastname: Feng
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Tong
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Yamashita
authors.forename: Jiang-nan
authors.initials: JN
authors.lastname: Yu
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D007279:Injections, Subcutaneous; D008055:Lipids; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage*; D011897:Random Allocation; D013752:Tetracycline / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D014018:Tissue Distribution / Q000187:drug effects / Q000502:physiology
chemical_list: D008055:Lipids; D013752:Tetracycline