title: Rationally designed fluorescence 'turn-on' sensor for Cu2+.
abstract: A rationally designed, coumarin-based fluorescent sensor imino-coumarin (IC) displays high selectivity for Cu(2+) over a variety of competing metal ions in aqueous solution with a significant fluorescence increase. DFT/TDDFT calculations support that the fluorescence 'turn-on' of IC originates from blocking the electron transfer of the nitrogen lone pair upon complexation with Cu(2+). IC was successfully applied to microscopic imaging for detection of Cu(2+) in LLC-MK2 cells (in vitro) and several living organs (in vivo).
authors.affiliation: Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Kyoung Chul
authors.initials: KC
authors.lastname: Ko
authors.forename: Jia-Sheng
authors.initials: JS
authors.lastname: Wu
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Pil Seung
authors.initials: PS
authors.lastname: Kwon
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Richard A
authors.initials: RA
authors.lastname: Bartsch
authors.forename: Jin Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D003300:Copper / Q000032:analysis*; D003374:Coumarins / Q000737:chemistry; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008253:Macaca mulatta; D051379:Mice; D008856:Microscopy, Fluorescence; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D007477:Ions; D003300:Copper; C030123:coumarin