title: [Forensic analysis of LCN DNA using sample concentration methods followed by miniSTR genotyping].
abstract: OBJECTIVE
  To optimize low copy number (LCN) DNA analysis methods for forensic STR genotyping.
  
  
  METHODS
  Two groups of DNA sample, extracted using either Magnetic bead method or Chelex-100 methods, were previously amplified with a Identifiler PCR Amplification kit, but no genotype was detected. The DNA samples were concentrated using either a drying method or the Microcon-100 method, then amplified using an miniFiler PCR Amplification kit and genotyped.
  
  
  RESULTS
  Among the 127 DNA samples, 47 samples, previously extracted using the Magnetic bead method, were genotyped with 36% success rate. Eighty samples, previously extracted using the Chelex-100 method, were genotyped with 30% success rate.
  
  
  CONCLUSIONS
  The application of sample concentration methods and miniFiler kit can improve the success rate of LCN STR analysis.
authors.affiliation: Shanghai Key Laboratory of Crime Science Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China. crislh@eastday.com
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Gu
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Lian-Kang
authors.initials: LK
authors.lastname: Chen
authors.forename: Huai-Gu
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D001801:Blood Stains; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D017931:DNA Primers; D053803:Forensic Genetics / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004247:DNA