title: In vivo graft performance of an improved bone substitute composed of poor crystalline hydroxyapatite based biphasic calcium phosphate.
abstract: The ability to promote new bone formation of poor crystalline hydroxyapatite (PC-HA) based biphasic calcium phosphate (BCP) bone substitutes were investigated. Various ratios of porous PC-HA/β-TCP (70/30, 60/40, and 0/100) grafts were fabricated. SEM and XRD measurements were performed to study the morphology and crystalline structure. Cylindrical artificial bone defects (3 × 6 mm(2)) were produced in alveolar bone at premolars extraction site and then filled with sterilized bone grafts. Commercial MBCP and unfilled empty defect served as control groups. At 8 weeks postoperation, samples were harvested from each artificial defect site for histological analysis. New bone formation of all the PC-HA/β-TCP groups was significantly greater than that of the empty control group (p<0.05), but without statistical difference from that of MBCP group. The degree of uniformity of new bone formation within defect region for PC-HA/β-TCP (60/40) was higher than that for MBCP. The PC-HA/β-TCP grafts showed enhanced bone regenerations with a more even dispersion of new bone formation than the other materials without causing inflammation, suggesting that these materials may be an alternative choice for bone void fillers in dental applications.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan, ROC.
authors.forename: Ching-Lien
authors.initials: CL
authors.lastname: Hung
authors.forename: Jen-Chang
authors.initials: JC
authors.lastname: Yang
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Chang
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Hu
authors.forename: Yong-Ho
authors.initials: YH
authors.lastname: Lin
authors.forename: Chun-Hsien
authors.initials: CH
authors.lastname: Huang
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Chen
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Lee
authors.forename: Nai-Chia
authors.initials: NC
authors.lastname: Teng
mesh_terms: D000539:Alveolar Process / Q000187:drug effects* / Q000601:surgery / Q000648:ultrastructure; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000737:chemistry; D002130:Calcium Phosphates / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D003460:Crystallization; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006882:Hydroxyapatites / Q000008:administration & dosage / Q000737:chemistry; D016348:Osseointegration / Q000187:drug effects*; D020390:Tooth Socket / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004338:Drug Combinations; D006882:Hydroxyapatites