title: MicroRNA-29a regulates pro-inflammatory cytokine secretion and scavenger receptor expression by targeting LPL in oxLDL-stimulated dendritic cells.
abstract: There is increasing evidence that microRNAs (miRNAs) play important roles in cell proliferation, apoptosis and differentiation that accompany inflammatory responses. However, whether microRNAs are associated with DC immuno-inflammatory responses with oxidized low density lipoprotein (oxLDL) stimulation is not yet known. Our study aims to explore the link of miRNAs with lipid-overload and immuno-inflammatory mechanism for atherosclerosis. In DCs transfected with microRNA-29a mimics or inhibitors, we showed that microRNA-29a plays an important role in proinflammatory cytokine secretion and scavenger receptor expression upon oxLDL-treatment. Furthermore, we suggest an additional explanation for the mechanism of microRNA-29a regulation of its functional target, lipoprotein lipase. We conclude that microRNA-29a could regulate pro-inflammatory cytokine secretion and scavenger receptor expression by targeting lipoprotein lipase in oxLDL-stimulated dendritic cells.
authors.affiliation: Department of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Zhoubin
authors.initials: Z
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Tu
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhu
authors.forename: Junhua
authors.initials: J
authors.lastname: Ge
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Zheng
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Pan
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
mesh_terms: D020413:3' Untranslated Regions; D050197:Atherosclerosis / Q000188:drug therapy / Q000276:immunology; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D058990:Molecular Targeted Therapy; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000378:metabolism; D051116:Receptors, Scavenger / Q000378:metabolism*; D051117:Scavenger Receptors, Class A / Q000235:genetics / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D018955:CD36 Antigens; D016207:Cytokines; D008077:Lipoproteins, LDL; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; C495137:OLR1 protein, human; D012333:RNA, Messenger; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein; C548261:LPL protein, human; D008071:Lipoprotein Lipase