title: A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue.
abstract: To evaluate the potential of three stem cells for cell therapy and tissue engineering applications, the biological behavior and osteogenic capacity of the newly introduced cord-blood-derived, unrestricted somatic stem cells (USSC) were compared with those of mesenchymal stem cells isolated from bone marrow (BM-MSC) and adipose tissue (AT-MSC). There was no significant difference between the rates of proliferation of the three stem cells. During osteogenic differentiation, alkaline phosphatase (ALP) activity peaked on day 7 in USSC compared to BM-MSC which showed the maximum value of ALP activity on day 14. However, BM-MSC had the highest ALP activity and mineralization during osteogenic induction. In addition, AT-MSC showed the lowest capacity for mineralization during differentiation and had the lowest ALP activity on days 7 and 14. Although AT-MSC expressed higher levels of collagen type I, osteonectin and BMP-2 in undifferentiated state, but these genes were expressed higher in BM-MSC during differentiation. BM-MSC also expressed higher levels of ALP, osteocalcin and Runx2 during induction. Taking together, BM-MSC showed the highest capacity for osteogenic differentiation and hold promising potential for bone tissue engineering and cell therapy applications.
authors.affiliation: Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Naser
authors.initials: N
authors.lastname: Ahmadbeigi
authors.forename: Peyman
authors.initials: P
authors.lastname: Dinarvand
authors.forename: Nasser
authors.initials: N
authors.lastname: Ghaemi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D024042:Collagen Type I / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics; D010012:Osteogenesis / Q000235:genetics / Q000502:physiology; D015676:Osteonectin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D023822:Tissue Engineering
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D015676:Osteonectin; D012333:RNA, Messenger; C493709:RUNX2 protein, human; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D002118:Calcium