title: Detection of bacillus Galmette-Guérin (Mycobacterium bovis BCG) DNA in urine and blood specimens after intravesical immunotherapy for bladder carcinoma.
abstract: A real-time PCR targeting IS6110 was employed for the detection of Mycobacterium tuberculosis DNA in specimens collected from 10 patients treated with intravesical M. bovis bacillus Galmette-Guérin (BCG) immunotherapy for bladder malignancy. BCG DNA was detected in all urine specimens taken 24 h after the instillations, as well as in 24% of the specimens collected 7 days after the instillations; it was also detected in a single specimen taken 6 weeks after the last instillation. BCG DNA was detected in 8.3% of the blood specimens taken 1 day after instillation, and its amplification was associated with cases of self-limiting fever. These findings give indications that this real-time PCR is helpful to recognize BCG bacteremic cases, which may lead to mycobacterial infection.
authors.affiliation: Department of Urology, Asclepeion General Hospital, 16673 Voula, Greece.
authors.forename: Argyrios
authors.initials: A
authors.lastname: Siatelis
authors.forename: Dimitra P
authors.initials: DP
authors.lastname: Houhoula
authors.forename: Joseph
authors.initials: J
authors.lastname: Papaparaskevas
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Delakas
authors.forename: Athanassios
authors.initials: A
authors.lastname: Tsakris
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001769:Blood / Q000382:microbiology; D002277:Carcinoma / Q000628:therapy*; D053487:DEAD-box RNA Helicases; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification / Q000652:urine*; D005260:Female; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000628:therapy*; D014556:Urine / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C448980:DDX50 protein, human; D053487:DEAD-box RNA Helicases