title: [Development of serological detection assays for human coronavirus HKU1 infection and its application].
abstract: OBJECTIVE
  To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection.
  
  
  METHODS
  The N protein of HCoV-HKU1 was expressed in E. Coli, anti-N antibody assay was established using Western Blotting with turn-based membrane. HCoV-HKU1 S protein was constructed in the eukaryotic expression plasmids, and confirmed by Western Blotting, S antibody assay was established using indirect immunofluorescence assay (IFA). We analyzed anti-S and anti-N antibody among 100 normal adult serum.
  
  
  RESULTS
  Expression of S and N protein were confirmed; 100 normal adult serum were analyzed using the established serological detection assay, in which HCoV-HKU1 S antibody positive rate was 47%, N antibody positive rate was 48%, Both S and N antibodies positive were 21%, Both S and N antibodies negative were 22%. Co-detection S and N antibody was achieved 74% positive rate.
  
  
  CONCLUSIONS
  The methods we established here could be used for serological analysis of HCoV-HKU1. Either detection of HCoV-HKU1 S or N antibodies achieved good results. Higher positive detection rate of anti-S or anti-N antibody was found in the normal adults.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Zhou
authors.forename: Wen-ling
authors.initials: WL
authors.lastname: Wang
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Tan
authors.forename: Ling-lin
authors.initials: LL
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Yin
authors.forename: Rou-jian
authors.initials: RJ
authors.lastname: Lu
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Wang
mesh_terms: D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D017934:Coronavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D018352:Coronavirus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D012698:Serologic Tests / Q000379:methods*; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus