title: Islet transplantation reverses the effects of maternal diabetes on mouse oocytes.
abstract: Maternal diabetes adversely affects preimplantation embryo development and oocyte maturation. Thus, it is important to identify ways to eliminate the effects of maternal diabetes on preimplantation embryos and oocytes. The objectives of this study were to investigate whether islet transplantation could reverse the effects of diabetes on oocytes. Our results revealed that maternal diabetes induced decreased ovulation; increased the frequency of meiotic spindle defects, chromosome misalignment, and aneuploidy; increased the relative expression levels of Mad2 and Bub1; and enhanced the sensitivity of oocytes to parthenogenetic activation. Islet transplantation prevented these detrimental effects. Therefore, we concluded that islet transplantation could reverse the effects of diabetes on oocytes, and that this technique may be useful to treat the fundamental reproductive problems of women with diabetes mellitus.
authors.affiliation: Organ Transplantation Institute of Xiamen University, Xiamen University, Xiamen City, Fujian Province 361005, People's Republic of China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Cheng
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Xia
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wang
authors.forename: Ji-Bing
authors.initials: JB
authors.lastname: Chen
authors.forename: Fei-Yu
authors.initials: FY
authors.lastname: Wang
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Quan-Jun
authors.initials: QJ
authors.lastname: Zhang
authors.forename: Zhong-Quan
authors.initials: ZQ
authors.lastname: Qi
mesh_terms: D000782:Aneuploidy; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000502:physiology; D001835:Body Weight; D002869:Chromosome Aberrations / Q000196:embryology / Q000706:statistics & numerical data; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy; D005260:Female; D016381:Islets of Langerhans Transplantation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D010060:Ovulation / Q000502:physiology; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics / Q000517:prevention & control*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D013311:Streptozocin