title: Old World hantaviruses do not produce detectable amounts of dsRNA in infected cells and the 5' termini of their genomic RNAs are monophosphorylated.
abstract: dsRNA and 5'-triphosphate RNA are considered critical activators of the innate immune response because of their interaction with pattern recognition receptors. It has been reported that no dsRNA is detected in negative-sense RNA virus-infected cells and that Hantaan virus (HTNV) genomic RNA bears a 5' monophosphate group. In this paper we examine the 5' termini of genomic RNAs of and dsRNA production by two major groups of Old World hantaviruses. No detectable amounts of dsRNA were found in infected cells. Also, the genomic RNAs of these hantaviruses bear a 5' monophosphate group and therefore are unable to trigger interferon induction. Taken together with the earlier data on HTNV, these results suggest that in addition to the dsRNA and genomic RNA, which may be only minimally involved in the induction of innate immunity, other cellular signalling pathways may also be involved and that these await further investigation.
authors.affiliation: Department of Virology, Infection Biology Research Program, Haartman Institute, University of Helsinki, Finland.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Virology, Infection Biology Research Program, Haartman Institute, University of Helsinki, Finland.
authors.forename: Antti
authors.initials: A
authors.lastname: Vaheri
authors.affiliation: Institute of Virology, Philipps University Marburg, Marburg, Germany.
authors.forename: Friedemann
authors.initials: F
authors.lastname: Weber
authors.affiliation: Department of Virology, Infection Biology Research Program, Haartman Institute, University of Helsinki, Finland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Plyusnin
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D018063:Hantaan virus / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D010758:Phosphorus / Q000032:analysis; D010766:Phosphorylation; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D010758:Phosphorus