title: Postoperative inflammation in the abdominal cavity increases adhesion formation in a laparoscopic mouse model.
abstract: OBJECTIVE
  To investigate acute inflammation in the peritoneal cavity in adhesion formation.
  
  
  METHODS
  Prospective randomized, controlled trial.
  
  
  METHODS
  University laboratory research center.
  
  
  METHODS
  9- to 10-week-old BALB/c female mice.
  
  
  METHODS
  In a laparoscopic mouse model, acute inflammation in the peritoneal cavity evaluated in CO(2) pneumoperitoneum enhanced adhesions, by CO(2) pneumoperitoneum plus manipulation, and in the latter group plus dexamethasone.
  
  
  METHODS
  Qualitative and quantitative adhesion scores and an acute inflammation score (neoangiogenesis, diapedesis, and leukocyte accumulation).
  
  
  RESULTS
  Adhesions at the lesion site were enhanced by the CO(2) pneumoperitoneum, further enhanced by manipulation, and decreased by the administration of dexamethasone. The acute inflammation scores (total, neoangiogenesis, diapedesis, and leukocyte accumulation) strongly correlated with the total adhesion score. Inflammation scores were similar at both the surgical lesion and the parietal peritoneum.
  
  
  CONCLUSIONS
  Acute inflammation of the entire peritoneum cavity is an important mechanism involved in adhesion formation and enhances adhesion formation at the lesion site.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium. coronaroberta@gmail.com
authors.forename: Roberta
authors.initials: R
authors.lastname: Corona
authors.forename: Jasper
authors.initials: J
authors.lastname: Verguts
authors.forename: Ron
authors.initials: R
authors.lastname: Schonman
authors.forename: Maria Mercedes
authors.initials: MM
authors.lastname: Binda
authors.forename: Karina
authors.initials: K
authors.lastname: Mailova
authors.forename: Philippe Robert
authors.initials: PR
authors.lastname: Koninckx
mesh_terms: D034841:Abdominal Cavity / Q000473:pathology*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D010535:Laparoscopy / Q000009:adverse effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010538:Peritonitis / Q000209:etiology / Q000473:pathology*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects; D011183:Postoperative Complications / Q000209:etiology / Q000473:pathology*; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology*