title: Performance of the Abbott RealTime HIV-1 viral load assay is not impacted by integrase inhibitor resistance-associated mutations.
abstract: The Abbott RealTime HIV-1 viral load assay uses primers and probes targeted to integrase, which is also the target of integrase inhibitors such as raltegravir. Viral loads of 42 raltegravir-susceptible and 40 raltegravir-resistant specimens were determined using RealTime HIV-1 and Roche Monitor (v1.5). The differences in viral load measurements between assays were comparable in the two groups, demonstrating that the RealTime HIV-1 assay can tolerate raltegravir-selected mutations.
authors.affiliation: Abbott Molecular, 1300 East Touhy Avenue, Des Plaines, IL 60018-3315, USA. tom.young@abbott.com
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Young
authors.forename: Gavin
authors.initials: G
authors.lastname: Cloherty
authors.forename: Signe
authors.initials: S
authors.lastname: Fransen
authors.forename: Laura
authors.initials: L
authors.lastname: Napolitano
authors.forename: Priscilla
authors.initials: P
authors.lastname: Swanson
authors.forename: Christine
authors.initials: C
authors.lastname: Herman
authors.forename: Neil T
authors.initials: NT
authors.lastname: Parkin
authors.forename: John
authors.initials: J
authors.lastname: Hackett
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology; D024882:Drug Resistance, Viral; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology*; D019427:HIV Integrase / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020125:Mutation, Missense; D011760:Pyrrolidinones / Q000494:pharmacology; D000068898:Raltegravir Potassium; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D019562:Viral Load / Q000379:methods*
chemical_list: D019380:Anti-HIV Agents; D011760:Pyrrolidinones; D011933:Reagent Kits, Diagnostic; D000068898:Raltegravir Potassium; D019427:HIV Integrase; C514801:p31 integrase protein, Human immunodeficiency virus 1