title: Rapid determination of rifaximin on dried blood spots by LC-ESI-MS.
abstract: The use of blood spot collection cards is a simple way to obtain specimens for therapeutic drug monitoring, assessing adherence to medications and preventing toxicity in a clinical setting. A high-throughput liquid chromatography-electrospray ionization mass spectrometric (LC-ESI-MS) method for determination of rifaximin on dried blood spots (DBS) was developed and validated. It involves solvent extraction of a punch of DBS followed by reversed-phase LC on a monolithic column consisting of a silica rod with bimodal pore structure and detection by ESI-MS. Rifampicin was used as an internal standard (IS). The run time was within 5.0 min with a very low back-pressure at a flow rate of 0.5 mL/min. The assay was linear from 0.1 to 10 ng/mL. The mean recovery was 98.42%. The developed method is very simple, rapid and useful for clinical applications.
authors.affiliation: Analytical Chemistry Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, 500 607, India. rnrao55@yahoo.com.
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: R Mastan
authors.initials: RM
authors.lastname: Vali
authors.forename: Bondigalla
authors.initials: B
authors.lastname: Ramachandra
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Maurya
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D059788:Dried Blood Spot Testing / Q000379:methods*; D004355:Drug Stability; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012294:Rifamycins / Q000097:blood* / Q000737:chemistry; D000078262:Rifaximin; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D012294:Rifamycins; D000078262:Rifaximin