title: The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins.
abstract: [FeFe]-hydrogenases have been claimed as the most promising catalysts of hydrogen bioproduction and several efforts have been accomplished to express and purify them. However, previous attemps to obtain a functional recombinant [FeFe]-hydrogenase in heterologous systems such as Escherichia coli failed due to the lack of the specific maturation proteins driving the assembly of its complex active site. The unique exception is that of [FeFe]-hydrogenase from Clostridium pasteurianum that has been expressed in active form in the cyanobacterium Synechococcus PCC 7942, which holds a bidirectional [NiFe]-hydrogenase with a well characterized maturation system, suggesting that the latter is flexible enough to drive the synthesis of a [FeFe]-enzyme. However, the capability of cyanobacteria to correctly fold a [FeFe]-hydrogenase in the absence of its auxiliary maturation proteins is a debated question. In this work, we expressed the [FeFe]-hydrogenase from Chlamydomonas reinhardtii as an active enzyme in the cyanobacterium Synechocystis sp. PCC 6803. Our results, using a different experimental system, confirm that cyanobacteria are able to express a functional [FeFe]-hydrogenase even in the absence of additional chaperones.
authors.affiliation: Department of Biology, University of Padova, 35131 Padova, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Berto
authors.forename: Sarah
authors.initials: S
authors.lastname: D'Adamo
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Bergantino
authors.forename: Francesca
authors.initials: F
authors.lastname: Vallese
authors.forename: Giorgio Mario
authors.initials: GM
authors.lastname: Giacometti
authors.forename: Paola
authors.initials: P
authors.lastname: Costantini
mesh_terms: D003013:Clostridium / Q000201:enzymology*; D006859:Hydrogen / Q000378:metabolism; D006864:Hydrogenase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D046939:Synechocystis / Q000201:enzymology*
chemical_list: D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; D006859:Hydrogen; C118830:iron hydrogenase; D006864:Hydrogenase