title: Basophil activation test for the diagnosis of hymenoptera venom allergy in childhood: a pilot study.
abstract: BACKGROUND
  Cellular in vitro tests such as the CD63-based basophil activation test (BAT) have been successfully used to diagnose hymenoptera venom sensitization in adult patients while this has not been investigated in children so far.
  
  
  METHODS
  15 children (9 male, 6 female; 12.7±3.5 years) with suspected allergy to vespula (VE) or honey bee (HB) venom entered this study. Besides serum tryptase (ST) levels, sensitisation against VE and HB was assessed by titrated skin testing and determination of venom-specific serum IgE (sIgE) in all patients. After stimulation with 50 ng of insect venom, CD63-expression of activated basophils was measured by flow cytometry.
  
  
  RESULTS
  Skin testing permitted identification of the culprit insect in 7 patients, 3 cases were diagnosed by additional sIgE measurements. In addition, BAT identified mono-sensitization in 3 further patients with double sensitization upon skin and sIgE testing. Test sensitivity was lower for the BAT (67-75%) than for skin testing (89-100%) and sIgE determination (100%). Neither basophil activation nor sIgE serum levels were identified as reliable predictors of sting reaction severity. In all patients, ST measurements yielded values below the upper reference value.
  
  
  CONCLUSIONS
  The current pilot study suggests a possible clinical benefit of BAT analysis in the diagnostic workup of pediatric insect venom allergy. However, further large-scale trials are required to investigate whether the BAT reliably contributes to the correct identification of the culprit insect venom. Due to its comparatively low sensitivity, the BAT should currently not be used in isolation from, but only in combination with established diagnostic instruments.
authors.affiliation: RWTH Aachen University, Department of Dermatology and Allergology, Aachen, Germany. hagen.ott@post.rwth-aachen.de
authors.forename: H
authors.initials: H
authors.lastname: Ott
authors.forename: K
authors.initials: K
authors.lastname: Tenbrock
authors.forename: J
authors.initials: J
authors.lastname: Baron
authors.forename: H
authors.initials: H
authors.lastname: Merk
authors.forename: S
authors.initials: S
authors.lastname: Lehmann
mesh_terms: D000293:Adolescent; D000707:Anaphylaxis / Q000175:diagnosis* / Q000276:immunology*; D015549:Basophil Degranulation Test; D001514:Bee Venoms / Q000276:immunology*; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood; D007299:Insect Bites and Stings / Q000150:complications* / Q000276:immunology*; D007428:Intradermal Tests; D008297:Male; D010865:Pilot Projects; D011237:Predictive Value of Tests; D014862:Wasp Venoms / Q000276:immunology*
chemical_list: D001514:Bee Venoms; D014862:Wasp Venoms; D007073:Immunoglobulin E