title: [Establishment of microarray for detecting mutation in HBV pre-core/core and basic core promoter regions].
abstract: OBJECTIVE
  To develop a sensitive and specific microarray for detecting mutations of HBV pre-core/core and basic core promoter regions in the clinic.
  
  
  METHODS
  Site-specific oligonucleotide probes were designed and immobilized to microarray slides and hybridized to HBV gene fragments amplified with specific biotin-labeled primer using asymmetrical PCR. The specificity and sensitivity of the method were estimated. And the microarray was applied to detect 138 clinical serum samples with HBV-DNA.
  
  
  RESULTS
  The mutations of HBV pre-core/core and basic core promoter regions can be specifically detected using the microarray, and the sensitivity was 1 x 10(1) copies/microl. Among 138 samples, 40 samples had T1762/ A1764 mutation, 11 samples had C1814 mutation, and 16 samples had A1896 mutation. The A1896 mutation rate in high HBV-DNA load group was significantly higher than that in low HBV-DNA load group (P < 0.01).
  
  
  CONCLUSIONS
  An DNA microarray assay was successfully established to detect the mutations in HBV pre-core/core and basic core promoter regions. The A1896 mutation in pre-core/core region maybe involve in duplication of HBV.
authors.affiliation: Xiaoshan Fourth People's Hospitail of Hangzhou, Hangzhou 311225, China. agw138@163.com
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Fang
authors.forename: Le-xiang
authors.initials: LX
authors.lastname: Lai
authors.forename: Yi-quan
authors.initials: YQ
authors.lastname: Le
authors.forename: Ling-jun
authors.initials: LJ
authors.lastname: Ren
mesh_terms: D000328:Adult; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011401:Promoter Regions, Genetic; D014758:Viral Core Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D014758:Viral Core Proteins