title: Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy.
abstract: BACKGROUND
  The Na+,K+-ATPase plays an important role for ion homeostasis in virtually all mammalian cells, including neurons. Despite this, there is as yet little known about the isoform specific distribution in neurons.
  
  
  RESULTS
  With help of superresolving stimulated emission depletion microscopy the spatial distribution of Na+,K+-ATPase in dendritic spines of cultured striatum neurons have been dissected. The found compartmentalized distribution provides a strong evidence for the confinement of neuronal Na+,K+-ATPase (α3 isoform) in the postsynaptic region of the spine.
  
  
  CONCLUSIONS
  A compartmentalized distribution may have implications for the generation of local sodium gradients within the spine and for the structural and functional interaction between the sodium pump and other synaptic proteins. Superresolution microscopy has thus opened up a new perspective to elucidate the nature of the physiological function, regulation and signaling role of Na+,K+-ATPase from its topological distribution in dendritic spines.
authors.affiliation: Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Blom
authors.forename: Daniel
authors.initials: D
authors.lastname: Rönnlund
authors.forename: Lena
authors.initials: L
authors.lastname: Scott
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Spicarova
authors.forename: Jerker
authors.initials: J
authors.lastname: Widengren
authors.forename: Alexander
authors.initials: A
authors.lastname: Bondar
authors.forename: Anita
authors.initials: A
authors.lastname: Aperia
authors.forename: Hjalmar
authors.initials: H
authors.lastname: Brismar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000201:enzymology* / Q000648:ultrastructure; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D007527:Isoenzymes / Q000378:metabolism / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D007527:Isoenzymes; C541459:Atp1a3 protein, rat; D000254:Sodium-Potassium-Exchanging ATPase