title: Collagen-based films containing liposome-loaded usnic acid as dressing for dermal burn healing.
abstract: The aim of this study was assess the effect of collagen-based films containing usnic acid as a wound dressing for dermal burn healing. Second-degree burn wounds were performed in forty-five Wistar rats, assigned into nine groups: COL-animals treated with collagen-based films; PHO-animals treated with collagen films containing empty liposomes; UAL-animals treated with collagen-based films containing usnic acid incorporated into liposomes. After 7, 14, and 21 days the animals were euthanized. On 7th day there was a moderate infiltration of neutrophils, in UAL, distributed throughout the burn wounds, whereas in COL and PHO, the severity of the reaction was slighter and still limited to the margins of the burn wounds. On the 14th day, the inflammatory reaction was less intense in UAL, with remarkable plasma cells infiltration. On the 21st day, there was reduction of the inflammation, which was predominantly composed of plasma cells in all groups, particularly in UAL. The use of the usnic acid provided more rapid substitution of type-III for type-I collagen on the 14th day, and improved the collagenization density on the 21st day. It was concluded that the use of reconstituted bovine type-I collagen-based films containing usnic acid improved burn healing process in rats.
authors.affiliation: Departamento de Fisiologia, Universidade Federal de Sergipe, Avenida Marechal Rondon s/n, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Paula S
authors.initials: PS
authors.lastname: Nunes
authors.forename: Ricardo L C
authors.initials: RL
authors.lastname: Albuquerque
authors.forename: Danielle R R
authors.initials: DR
authors.lastname: Cavalcante
authors.forename: Marx D M
authors.initials: MD
authors.lastname: Dantas
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Cardoso
authors.forename: Marília S
authors.initials: MS
authors.lastname: Bezerra
authors.forename: Jamille C C
authors.initials: JC
authors.lastname: Souza
authors.forename: Mairim Russo
authors.initials: MR
authors.lastname: Serafini
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quitans
authors.forename: Leonardo R
authors.initials: LR
authors.lastname: Bonjardim
authors.forename: Adriano A S
authors.initials: AA
authors.lastname: Araújo
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage*; D001684:Biological Dressings; D002056:Burns / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D003094:Collagen / Q000008:administration & dosage*; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control; D008081:Liposomes / Q000008:administration & dosage*; D008297:Male; D058628:Myofibroblasts / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000199:Actins; D001572:Benzofurans; D003094:Collagen; D024042:Collagen Type I; D024061:Collagen Type III; D008081:Liposomes; C498862:Acta2 protein, rat; C073339:usnic acid