title: Retrofit implementation of Zernike phase plate imaging for cryo-TEM.
abstract: In-focus phase-plate imaging is particularly beneficial for cryo-TEM because it offers a substantial overall increase in image contrast, without an electron dose penalty, and it simplifies image interpretation. We show how phase-plate cryo-TEM can be implemented with an appropriate existing TEM, and provide a basic practical introduction to use of thin-film (carbon) phase plates. We point out potential pitfalls of phase-plate operation, and discuss solutions. We provide information on evaluating a particular TEM for its suitability.
authors.affiliation: Resource for Visualization of Biological Complexity, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509, USA. marko@wadsworth.org
authors.forename: Michael
authors.initials: M
authors.lastname: Marko
authors.forename: Ardean
authors.initials: A
authors.lastname: Leith
authors.forename: Chyongere
authors.initials: C
authors.lastname: Hsieh
authors.forename: Radostin
authors.initials: R
authors.lastname: Danev
mesh_terms: D000818:Animals; D020285:Cryoelectron Microscopy / Q000295:instrumentation* / Q000379:methods; D055032:Electron Microscope Tomography / Q000295:instrumentation* / Q000379:methods; D008099:Liver / Q000648:ultrastructure; D051381:Rats; D014584:User-Computer Interface