title: Role of Bcr1-activated genes Hwp1 and Hyr1 in Candida albicans oral mucosal biofilms and neutrophil evasion.
abstract: Candida albicans triggers recurrent infections of the oropharyngeal mucosa that result from biofilm growth. Prior studies have indicated that the transcription factor Bcr1 regulates biofilm formation in a catheter model, both in vitro and in vivo. We thus hypothesized that Bcr1 plays similar roles in the formation of oral mucosal biofilms and tested this hypothesis in a mouse model of oral infection. We found that a bcr1/bcr1 mutant did not form significant biofilm on the tongues of immunocompromised mice, in contrast to reference and reconstituted strains that formed pseudomembranes covering most of the tongue dorsal surface. Overexpression of HWP1, which specifies an epithelial adhesin that is under the transcriptional control of Bcr1, partly but significantly rescued the bcr1/bcr1 biofilm phenotype in vivo. Since HWP1 overexpression only partly reversed the biofilm phenotype, we investigated whether additional mechanisms, besides adhesin down-regulation, were responsible for the reduced virulence of this mutant. We discovered that the bcr1/bcr1 mutant was more susceptible to damage by human leukocytes when grown on plastic or on the surface of a human oral mucosa tissue analogue. Overexpression of HYR1, but not HWP1, significantly rescued this phenotype. Furthermore a hyr1/hyr1 mutant had significantly attenuated virulence in the mouse oral biofilm model of infection. These discoveries show that Bcr1 is critical for mucosal biofilm infection via regulation of epithelial cell adhesin and neutrophil function.
authors.affiliation: Department of Microbiology, University of Texas, Houston, Texas, United States of America.
authors.forename: Prabhat
authors.initials: P
authors.lastname: Dwivedi
authors.forename: Angela
authors.initials: A
authors.lastname: Thompson
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Xie
authors.forename: Helena
authors.initials: H
authors.lastname: Kashleva
authors.forename: Shantanu
authors.initials: S
authors.lastname: Ganguly
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Mitchell
authors.forename: Anna
authors.initials: A
authors.lastname: Dongari-Bagtzoglou
mesh_terms: D018829:Adhesins, Bacterial / Q000502:physiology; D000818:Animals; D018441:Biofilms; D002176:Candida albicans / Q000276:immunology / Q000472:pathogenicity*; D005656:Fungal Proteins / Q000502:physiology*; D057131:Immune Evasion; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D009061:Mouth Mucosa / Q000382:microbiology*; D009504:Neutrophils / Q000276:immunology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D005656:Fungal Proteins; C118102:HWP1 protein, Candida albicans; C101636:HYR1 protein, Candida albicans; D008562:Membrane Glycoproteins; D014157:Transcription Factors