title: Specific biotinylation of IMP dehydrogenase.
abstract: IMP dehydrogenase (IMPDH) catalyzes a critical step in guanine nucleotide biosynthesis. IMPDH also has biological roles that are distinct from its enzymatic function. We report a biotin-linked reagent that selectively labels IMPDH and is released by dithiothreitol. This reagent will be invaluable in elucidating the moonlighting functions of IMPDH.
authors.affiliation: Graduate Program in Biochemistry, Brandeis University, Waltham, MA 02454, USA.
authors.forename: B Christopher
authors.initials: BC
authors.lastname: Hoefler
authors.forename: Deviprasad R
authors.initials: DR
authors.lastname: Gollapalli
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Hedstrom
mesh_terms: D019921:Biotinylation; D004229:Dithiothreitol / Q000737:chemistry; D006801:Humans; D007168:IMP Dehydrogenase / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure
chemical_list: D007168:IMP Dehydrogenase; D004229:Dithiothreitol