title: Quercetin reduces neutrophil recruitment induced by CXCL8, LTB4, and fMLP: inhibition of actin polymerization.
abstract: Recent in vitro data have suggested that the flavonoid quercetin (1) does not affect the functioning of neutrophils. Therefore, we evaluated in vivo and in vitro whether or not 1 affects neutrophil function, focusing on recruitment. The in vivo treatment with 1 inhibited in a dose-dependent manner the recruitment of neutrophils to the peritoneal cavity of mice induced by known chemotatic factors such as CXCL1, CXCL5, LTB(4), and fMLP. Furthermore, 1 also inhibited in a concentration-dependent manner the chemoattraction of human neutrophils induced by CXCL8, LTB(4), and fMLP in a Boyden chamber. In vitro treatment with 1 did not affect human neutrophil surface expression of CXCR1, CXCR2, BLT1, or FLPR1, but rather reduced actin polymerization. These results suggest that 1 inhibits actin polymerization, hence, explaining the inhibition of neutrophil recruitment in vivo and in vitro and highlighting its possible usefulness to diminish excessive neutrophil migration during inflammation.
authors.affiliation: Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
authors.forename: Fabricio O
authors.initials: FO
authors.lastname: Souto
authors.forename: Ana C
authors.initials: AC
authors.lastname: Zarpelon
authors.forename: Larissa
authors.initials: L
authors.lastname: Staurengo-Ferrari
authors.forename: Victor
authors.initials: V
authors.lastname: Fattori
authors.forename: Rubia
authors.initials: R
authors.lastname: Casagrande
authors.forename: Maria J V
authors.initials: MJ
authors.lastname: Fonseca
authors.forename: Thiago M
authors.initials: TM
authors.lastname: Cunha
authors.forename: Sergio H
authors.initials: SH
authors.lastname: Ferreira
authors.forename: Fernando Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: Waldiceu A
authors.initials: WA
authors.lastname: Verri
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D054365:Chemokine CXCL5 / Q000276:immunology*; D002630:Chemotactic Factors / Q000276:immunology; D002633:Chemotaxis / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007249:Inflammation / Q000276:immunology; D016209:Interleukin-8 / Q000187:drug effects* / Q000276:immunology; D007975:Leukotriene B4 / Q000276:immunology*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology*; D020556:Neutrophil Infiltration / Q000187:drug effects* / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D011794:Quercetin / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000199:Actins; C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D002630:Chemotactic Factors; D016209:Interleukin-8; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011794:Quercetin