title: Synthetically functionalized retroviruses produced from the bioorthogonally engineered cell surface.
abstract: Conjugation of desired molecules onto retroviral surfaces through the ease of the bioorthogonal functionalization method was demonstrated. Oxidation of surface sialic acids using periodate and further p-anisidine-catalyzed conjugation with aminooxy-bearing molecules were used to directly label retroviral envelope with a fluorescent dye. The retroviral particles that were produced from a bioorthogonally functionalized virus producing cell surface and further tethered with magnetic nanoparticles were efficiently purified by simple magnetic column separation and capable of magnet-directed transduction.
authors.affiliation: Medicinal Chemistry and Pharmacology Program, University of California, Irvine, California 92697, United States.
authors.forename: Shirley
authors.initials: S
authors.lastname: Wong
authors.forename: Young Jik
authors.initials: YJ
authors.lastname: Kwon
mesh_terms: D000588:Amines / Q000737:chemistry; D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D002384:Catalysis; D005456:Fluorescent Dyes / Q000737:chemistry; D008280:Magnetics; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D012190:Retroviridae / Q000737:chemistry / Q000378:metabolism*; D012794:Sialic Acids / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D000588:Amines; D000814:Aniline Compounds; D005456:Fluorescent Dyes; D012794:Sialic Acids; D014764:Viral Proteins; C013813:4-anisidine