title: Zebrafish HSC70 promoter to express carp muscle-specific creatine kinase for acclimation under cold condition.
abstract: Zebrafish (Danio rerio) is used as a model system for in vivo studies. To expand the research scope of physical, biochemical and physiological studies, a cold-tolerant model of zebrafish was developed. The common carp (Cyprinus carpio) muscle form of creatine kinase (CK, EC 2.7.3.2) can maintain enzymatic activity at a temperature of around 15°C. However, a cold-inducible promoter of zebrafish, hsc 70 (heat shock protein 70 cognate), is able to increase the expression of gene product by 9.8 fold at a temperature of 16°C. Therefore, the carp CK gene was promoted by hsc 70 and transfected into zebrafish embryos. Resulting transgenic zebrafish survived and could maintain its swimming behavior at 13°C, which was not possible with the wild-type zebrafish. The swimming distance of the transgenic fish was 42% greater than that of the wild type at 13°C. This new transgenic fish model is ideal for studies of ectothermal vertebrates in low-temperature environments.
authors.affiliation: Institute of Cellular and Organismic Biology, 128 Academia Road, Sect. 2 Academia Sinica, Taipei 115, Taiwan.
authors.forename: Chih-Lu
authors.initials: CL
authors.lastname: Wu
authors.forename: Ta-Hui
authors.initials: TH
authors.lastname: Lin
authors.forename: Tien-Lin
authors.initials: TL
authors.lastname: Chang
authors.forename: Hsi-Wen
authors.initials: HW
authors.lastname: Sun
authors.forename: Cho-Fat
authors.initials: CF
authors.lastname: Hui
authors.forename: Jen-Leih
authors.initials: JL
authors.lastname: Wu
mesh_terms: D000064:Acclimatization; D000818:Animals; D030801:Animals, Genetically Modified; D002347:Carps / Q000235:genetics*; D003080:Cold Temperature; D003402:Creatine Kinase / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D004789:Enzyme Activation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008845:Microinjections; D023421:Models, Animal; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013550:Swimming / Q000502:physiology; D014162:Transfection; D019076:Transgenes; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D050883:HSC70 Heat-Shock Proteins; D029961:Zebrafish Proteins; C494374:hspa8 protein, zebrafish; D003402:Creatine Kinase