title: [Sequence analysis of a novel human leukocyte antigen allele B*5827].
abstract: OBJECTIVE
  To investigate the molecular basis for a novel human leukocyte antigen (HLA) allele B*5827.
  
  
  METHODS
  DNA from the proband was analyzed by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) typing. The amplified product was sequenced bidirectionally.
  
  
  RESULTS
  Abnormal HLA-B locus was observed and its nucleotide sequence was different from the known HLA-B allele sequences, with highest homology to HLA-B*5820 allele. It differs from HLA-B*5820 by 8 nucleotide substitutions in exon 3, i.e., nt 290 (G > C), nt 346 (T > A), nt 390 (A > C), nt 404 (G > C), nt 413 (C > G), nt 471 (A > G), nt 486 (A > G) and nt 487 (C > A), resulting in an amino acid change from ser > arg at nt 97, phe >tyr at nt 115, ser > arg at nt 130, thr > ala at nt 157 and thr > glu at nt 162. Nucleotide differences of nt 404 (G > C) and nt 413( C > G) did not change amino acid.
  
  
  CONCLUSIONS
  The sequences of the novel allele have been submitted to GenBank (access No.GU071234). A novel HLA class I allele B*5827 has been officially assigned by the WHO HLA Nomenclature Committee in Jan. 2010.
authors.affiliation: Shandong Umbilical Cord Blood Bank, Jinan, Shandong, 250102 PR China.
authors.forename: Chao-xia
authors.initials: CX
authors.lastname: Lu
authors.forename: Na
authors.initials: N
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.forename: Bing-shen
authors.initials: BS
authors.lastname: Ke
authors.forename: Huai-shui
authors.initials: HS
authors.lastname: Hou
authors.forename: Bai-jun
authors.initials: BJ
authors.lastname: Shen
mesh_terms: D000483:Alleles; D001483:Base Sequence; D003001:Cloning, Molecular; D005838:Genotype; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens