title: Integrated refocused virtual ESEEM: detection of nuclear transition spectra without dead time and blind spots.
abstract: General expressions describing the refocused stimulated (RS) and refocused virtual (RV) electron spin echo envelope modulations (ESEEM) generated with the same basic four-pulse sequence are derived. It is shown that integration of the 3D time domain trace over the two "low-resolution" time intervals (those between the first and second and between the third and fourth microwave pulses) results in a dead time-free 1D ESEEM trace in the "high-resolution" dimension (i.e., the time interval between the second and third microwave pulses) that only contains harmonics with the fundamental frequencies of nuclear transitions. The practical implementation of the integrated RS ESEEM requires pulse swapping, which leads to unrecoverable distortions in the ESEEM traces and the resulting spectra. The integrated RV ESEEM is free from such distortions and represents a robust practical technique for obtaining dead time- and blind spots-free spectra of nuclear transitions, without homonuclear combination lines. As an application example, the integrated RV ESEEM was used to obtain the spectrum of a strongly-coupled proton of the OH ligand of the Mo(V) active center of the low-pH form of the molybdoenzyme sulfite oxidase.
authors.affiliation: University of Arizona, Department of Chemistry and Biochemistry, Tucson, AZ 85721, USA. andrei@u.arizona.edu
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Astashkin
mesh_terms: D000465:Algorithms; D016477:Artifacts; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D018511:Systems Integration