title: Serial line scan encoding imaging cytometer for both adherent and suspended cells.
abstract: We present a new design of an imaging cytometer for high content bioanalysis, which is equipped with a low-cost linear complementary metal oxide semiconductor (CMOS) imager (running at ≥40kHz). The fluorescent signals are encoded in a series of line scans across the cellular body, while it streams through a precisely defined line-shaped focus spot. This bioanalysis platform enables the concurrent collection of multiple fluorescence channels, while maintaining both high resolution and excellent throughput (1000 cells/s). We develop several image processing routines for the on-the-fly quantitative analysis of subcellular structures. Finally, we characterize our prototype system by imaging both adherent cells (plate format) and suspended cells (microfluidics format).
authors.affiliation: Gene Expression Division, Bio-Rad Laboratories, Hercules, California 94547, United States. xin_heng@bio-rad.com
authors.forename: Xin
authors.initials: X
authors.lastname: Heng
authors.forename: Frank
authors.initials: F
authors.lastname: Hsiung
authors.forename: Amir
authors.initials: A
authors.lastname: Sadri
authors.forename: Paul
authors.initials: P
authors.lastname: Patt
mesh_terms: D005434:Flow Cytometry / Q000295:instrumentation*; D005453:Fluorescence; D044085:Microfluidics