title: Effects of supplemental UV-A on the development, anatomy and metabolite production of Phyllanthus tenellus cultured in vitro.
abstract: Phyllanthus tenellus is widely used for its antiviral, analgesic and hepatoprotective properties. Although the production of several chemical classes of secondary metabolites is influenced by UV radiation, particularly phenolic compounds, we also know that UV radiation can result in anatomical and developmental damage. However, the morphological, anatomical and phytochemical changes in response to UV-A exposure are generally understudied in the Phyllanthaceae. Therefore, we evaluated the effects of UV-A radiation on plant development and leaf anatomy, as well as the production of secondary metabolites and the contents of carotenoids and chlorophylls a and b, in P. tenellus. To accomplish this, in vitro cultures of P. tenellus were maintained for 60 days under white light (WL) and WL plus UV-A radiation. Results showed different phenotypic responses under additional UV-A, such as high phenolic metabolite production, increasing dimensions of abaxial epidermis and thickness of palisade parenchyma. Compared to plants cultured under WL, UV-A radiation caused damage to plant morphogenesis, including a reduced number of branches and shoots, consequently reducing the rate of proliferation. On the other hand, geraniin, ellagic acid and carotenoid contents increased after UV-A exposure, indicating that this light source is an important resource for inducing phenolic compounds.
authors.affiliation: Centro Universitário Estadual da Zona Oeste-UEZO, Colegiado de Ciências Biológicas e da Saúde, Campo Grande, RJ, Brazil. cris.pvictor@gmail.com
authors.forename: Cristiane Pimentel
authors.initials: CP
authors.lastname: Victório
authors.forename: Marcos Vinicius
authors.initials: MV
authors.lastname: Leal-Costa
authors.forename: Eliana Schwartz
authors.initials: ES
authors.lastname: Tavares
authors.forename: Ricardo Machado
authors.initials: RM
authors.lastname: Kuster
authors.forename: Celso Luiz Salgueiro
authors.initials: CL
authors.lastname: Lage
mesh_terms: D002338:Carotenoids / Q000032:analysis / Q000096:biosynthesis*; D002734:Chlorophyll / Q000032:analysis / Q000096:biosynthesis; D046508:Culture Techniques; D004610:Ellagic Acid / Q000032:analysis / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000096:biosynthesis*; D005960:Glucosides / Q000032:analysis / Q000096:biosynthesis; D047348:Hydrolyzable Tannins / Q000032:analysis; D008027:Light; D058503:Mesophyll Cells / Q000528:radiation effects; D029803:Phyllanthus / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D019441:Plant Epidermis / Q000528:radiation effects; D018515:Plant Leaves / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D018520:Plant Shoots / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D005419:Flavonoids; D005960:Glucosides; D047348:Hydrolyzable Tannins; D002734:Chlorophyll; D004610:Ellagic Acid; D002338:Carotenoids; C024603:Geraniin