title: Further characterization of Bacillus subtilis antibiotic biosensors and their use for antibacterial mode-of-action studies.
abstract: We further examined the usefulness of previously reported Bacillus subtilis biosensors for antibacterial mode-of-action studies. The biosensors could not detect the tRNA synthetase inhibitors mupirocin, indolmycin, and borrelidin, some inhibitors of peptidoglycan synthesis, and most membrane-damaging agents. However, the biosensors confirmed the modes of action of several RNA polymerase inhibitors and DNA intercalators and provided new insights into the possible modes of action of ciprofloxacin, anhydrotetracycline, corralopyronin, 8-hydroxyquinoline, and juglone.
authors.affiliation: Antimicrobial Research Centre and Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Mariner
authors.forename: Nicola
authors.initials: N
authors.lastname: Ooi
authors.forename: Deborah
authors.initials: D
authors.lastname: Roebuck
authors.forename: Alex J
authors.initials: AJ
authors.lastname: O'Neill
authors.forename: Ian
authors.initials: I
authors.lastname: Chopra
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000037:antagonists & inhibitors; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects*; D015374:Biosensing Techniques; D002939:Ciprofloxacin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005233:Fatty Alcohols / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D016712:Mupirocin / Q000494:pharmacology; D009285:Naphthoquinones / Q000494:pharmacology; D015125:Oxyquinoline / Q000494:pharmacology; D013754:Tetracyclines / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D005233:Fatty Alcohols; D007211:Indoles; D009285:Naphthoquinones; D013754:Tetracyclines; D002939:Ciprofloxacin; D015125:Oxyquinoline; C016229:4-epianhydrotetracycline; C005066:borrelidin; D016712:Mupirocin; D000604:Amino Acyl-tRNA Synthetases; C005134:juglone; C001447:indolmycin