title: Quality control of a herbal medicinal preparation using high-performance liquid chromatographic and capillary electrophoretic methods.
abstract: Two methods based on high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) were developed for the quality control of "samgiumgagambang" (SGMX), a new herbal medicinal preparation containing 14 herbs. Nine components from SGMX were selected as markers: 5-hydroxymethylfuraldehyde, geniposidic acid, chlorogenic acid, paeoniflorin, 20-hydroxyecdysone, coptisine, berberine, luteolin, and glycyrrhizic acid. The markers were identified and analyzed using HPLC coupled with a UV-diode-array detector and monitored at 250nm with a gradient elution of acetonitrile and water containing formic acid on a C(18) analytical column or using CE with a 70mM borate buffer (pH 9.5) containing 10% methanol on a 60-cm fused silica capillary monitored at 230nm. The marker components in SGMX were well separated using both methods and were readily determined within 60min using HPLC or 13min using CE with good precision and accuracy.
authors.affiliation: College of Pharmacy, Chungnam National University, Yooseong-Ku, Daejeon 305-764, Republic of Korea.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhu
authors.forename: Van Men
authors.initials: VM
authors.lastname: Chu
authors.forename: Yu Seon
authors.initials: YS
authors.lastname: Jang
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Son
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Chang Gyu
authors.initials: CG
authors.lastname: Son
authors.forename: In Chan
authors.initials: IC
authors.lastname: Seol
authors.forename: Jong Seong
authors.initials: JS
authors.lastname: Kang
mesh_terms: D002138:Calibration; D002318:Cardiovascular Diseases / Q000188:drug therapy / Q000517:prevention & control; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D057230:Limit of Detection; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D056910:Republic of Korea; D013678:Technology, Pharmaceutical; D013997:Time Factors
chemical_list: D010936:Plant Extracts; C558757:samgiumgagambang