title: Distribution of glycoproteins on feline testicular sperm, epididymal sperm and ejaculated sperm.
abstract: Glycoproteins (GPs) are known to be involved in the phenomenon of sperm maturation and capacitation. In the present study, we investigated the attachment of GPs on sperm cell membrane during the process of feline sperm maturation from testicular sperm to ejaculated sperm by using 8 FITC-labeled lectins. The results showed that 3 types of GPs were presented on testicular sperm and 7 on caput epididymal sperm. Corpus and cauda epididymal sperm and ejaculated sperm had GPs detected by 8 FITC-labeled lectins used in the present study. This study demonstrates the part of the characteristic of GPs that are present on the feline sperm cell membrane during the process of sperm maturation.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan. marimaru@sd5.so-net.ne.jp
authors.forename: Mari
authors.initials: M
authors.lastname: Toyonaga
authors.forename: Miki
authors.initials: M
authors.lastname: Morita
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D002415:Cats / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006023:Glycoproteins / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry*; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D037102:Lectins; D016650:Fluorescein-5-isothiocyanate