title: Paired box gene 5 may modulate Proviral Integration of Moloney virus 2 gene and protein expression in mature B-cells.
abstract: Deregulated transcription factor Paired box gene 5 (Pax-5) expression has been implicated in B-cell lymphoma in human disease. Proviral Integration of Moloney virus 2 (Pim-2) kinase may play a role in apoptosis and chemotherapy resistance. We characterized the expression of Pax-5 and Pim-2 in mature B-cell lines and 293T cells transfected with human isoforms of Pax-5. By quantitative real-time polymerase chain reaction (qRT-PCR), we showed that Pax-5 isoforms significantly increased Pim-2 mRNA expression in 293T cells and that mRNAs for both Pax-5 and Pim-2 were two-fold significantly expressed in mature malignant B-cells. Western blot analysis showed that Pim-2 protein expression was increased in 293T cells transiently transfected with human isoforms of Pax-5 and that Pax-5 isoforms and Pim-2 proteins were expressed in mature B-cell lines. By electrophoretic mobility shift analysis, we showed that Pax-5 isoforms were able to bind to the Pim-2 promoter region. Taken together, these results suggest that Pax-5 may act as a transcription factor to modulate the expression of Pim-2 and that both proteins may play a role in the oncogenesis of mature B-cell lymphoma.
authors.affiliation: Atlantic Cancer Research Institute, Hôtel-Dieu Pavilion, Moncton, NB, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Narayansingh
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Ouellette
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics; D051757:PAX5 Transcription Factor / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020033:Protein Isoforms; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D051757:PAX5 Transcription Factor; C497281:PAX5 protein, human; C490587:PIM2 protein, human; D020033:Protein Isoforms; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D017346:Protein Serine-Threonine Kinases