title: Structures of SAS-6 suggest its organization in centrioles.
abstract: Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers.
authors.affiliation: Medical Research Council-Laboratory of Molecular Biology (MRC-LMB), Hills Road, Cambridge, UK. vanbreug@mrc-lmb.cam.ac.uk
authors.forename: Mark
authors.initials: M
authors.lastname: van Breugel
authors.forename: Masafumi
authors.initials: M
authors.lastname: Hirono
authors.forename: Antonina
authors.initials: A
authors.lastname: Andreeva
authors.forename: Haru-aki
authors.initials: HA
authors.lastname: Yanagisawa
authors.forename: Shoko
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakazawa
authors.forename: Nina
authors.initials: N
authors.lastname: Morgner
authors.forename: Miriana
authors.initials: M
authors.lastname: Petrovich
authors.forename: Ima-Obong
authors.initials: IO
authors.lastname: Ebong
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Johnson
authors.forename: Dmitry
authors.initials: D
authors.lastname: Veprintsev
authors.forename: Benoît
authors.initials: B
authors.lastname: Zuber
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D002502:Centrioles / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D018385:Centrosome / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D005407:Flagella / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry; D017354:Point Mutation; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D050505:Mutant Proteins; D011994:Recombinant Proteins; C497202:SASS6 protein, human; D029961:Zebrafish Proteins; C525192:sass6 protein, zebrafish