title: [Preparation and identification of the monoclonal antibodies against programmed death-1 ligant 1].
abstract: OBJECTIVE
  To prepare monoclonal antibodies (McAbs) against human programmed death-1 ligant 1(PD-L1) and identify its bioactivity.
  
  
  METHODS
  We immunized the BALB/c mice with Thioredoxin-(PD-L1) recombination protein which expressed by prokaryotic system. Prepare hybridoma cell by hybridoma technology and used enzyme-linked immunosorbent assay(ELISA) and Western-blotting assays to select positive hybridoma identify cell. Competition inhibition ELISA was carried out to identify the special bioactivity of antibody.
  
  
  RESULTS
  4 hybridoma cell strains which could secrete anti-(PD-L1) antibodies stably were selected. The McAbs has good affinity with its receptor. Purify anti-(PD-L1) with title 1:32 000 was obtained after large quantity preparation. At the same time we obtained 1 cell stain which could secret special anti-Trx McAbs.
  
  
  CONCLUSIONS
  We obtained anti-(PD-L1) McAbs with good bioactivity successfully, which lay the foundation for further study.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Shen
authors.forename: Si-yong
authors.initials: SY
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Bian
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology*; D060890:B7-H1 Antigen; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human