title: [STR profiling of genomic DNA from HE stained tissue sections].
abstract: OBJECTIVE
  To develop a STR analysis method for analyzing DNA from stained tissue sections and to evaluate the capability of this protocol in forensic application.
  
  
  METHODS
  Eight kinds of HE stained human tissue, for example heart, liver, lung and intestine, were collected from two autopsy cases. The genomic DNA from those tissues was extracted using a QIAgen kit. DNA quantitation was performed using the TaqMan PCR method. The concentration of DNA isolated was determined based on Ct values. Internal positive controls (IPC) were used to monitor inhibitors. DNA amplifications were performed using Identifiler PCR Amplification kit. PCR products were analyzed on 3100-Avant Genetic Analyzer.
  
  
  RESULTS
  The concentrations of DNA obtained from all samples were greater than 1 ng/microL. PCR inhibition was not observed. However, DNA degradation, potentially due to the effect of residual formalin fixative, was observed among tissue samples stored for long periods of time.
  
  
  CONCLUSIONS
  Sufficient amounts of DNA were extracted from HE stained tissue sections. STR profiles were successfully generated. The number of genotype alleles detected decreased as sample storage time increased.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. qliuyan@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhen-Min
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Kai-Fei
authors.initials: KF
authors.lastname: Deng
mesh_terms: D000483:Alleles; D002102:Cadaver; D004247:DNA / Q000032:analysis* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005838:Genotype; D006801:Humans; D008099:Liver; D008168:Lung; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000379:methods; D013194:Staining and Labeling; D020080:Tandem Repeat Sequences
chemical_list: D004247:DNA