title: Proteomic analysis of articular cartilage vesicles from normal and osteoarthritic cartilage.
abstract: OBJECTIVE
  Articular cartilage vesicles (ACVs) are extracellular organelles found in normal articular cartilage. While they were initially defined by their ability to generate pathologic calcium crystals in cartilage of osteoarthritis (OA) patients, they can also alter the phenotype of normal chondrocytes through the transfer of RNA and protein. The purpose of this study was to analyze the proteome of ACVs from normal and OA human cartilage.
  
  
  METHODS
  ACVs were isolated from cartilage samples from 10 normal controls and 10 OA patients. We identified the ACV proteomes using in-gel trypsin digestion, nanospray liquid chromatography tandem mass spectrometry analysis of tryptic peptides, followed by searching an appropriate subset of the Uniprot database. We further differentiated between normal and OA ACVs by Holm-Sidak analysis for multiple comparison testing.
  
  
  RESULTS
  More than 1,700 proteins were identified in ACVs. Approximately 170 proteins satisfied our stringent criteria of having >1 representative peptide per protein present, and a false discovery rate of ≤5%. These proteins included extracellular matrix components, phospholipid binding proteins, enzymes, and cytoskeletal components, including actin. While few proteins were seen exclusively in normal or OA ACVs, immunoglobulins and complement components were present only in OA ACVs. Compared to normal ACVs, OA ACVs displayed decreases in matrix proteoglycans and increases in transforming growth factor β-induced protein βig-H3, DEL-1, vitronectin, and serine protease HtrA1 (P < 0.01).
  
  
  CONCLUSIONS
  These findings lend support to the concept of ACVs as physiologic structures in articular cartilage. Changes in OA ACVs are largely quantitative and reflect an altered matrix and the presence of inflammation, rather than revealing fundamental changes in composition.
authors.affiliation: Medical College of Wisconsin, Milwaukee, USA. ann.rosenthal@va.gov
authors.forename: Ann K
authors.initials: AK
authors.lastname: Rosenthal
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Gohr
authors.forename: James
authors.initials: J
authors.lastname: Ninomiya
authors.forename: Bassam T
authors.initials: BT
authors.lastname: Wakim
mesh_terms: D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008832:Microchemistry; D036103:Nanotechnology; D020370:Osteoarthritis, Knee / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology