title: Genetic diversity and population structure in French populations of Mycosphaerella graminicola.
abstract: Mycosphaerella graminicola populations were examined in France with microsatellite markers and PCR-SSCP analysis of partial actin and β-tubulin encoding sequences. A total of 363 isolates was sampled in 2005 from 17 provinces, and genotypes from corresponding strains were characterized. Unique haplotypes comprised 84% of the population, and gene diversity was high nationwide (0.70) and locally. A moderate genetic differentiation (G(ST) = 0.18) was found and indicated that in France the M. graminicola population was more structured than in other previously studied European countries. Bayesian structure analysis identified three genetic clusters distributed among the 17 provinces. Our results highlighted the potential for the adaptation of the fungus to local conditions, leading to genetic clusters among the French population of the fungus as well as genotype flow between regional clusters.
authors.affiliation: Université Lille-Nord de France, Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, B.P. 699, F-62228 Calais cedex, France.
authors.forename: Lea
authors.initials: L
authors.lastname: El Chartouni
authors.forename: Benoît
authors.initials: B
authors.lastname: Tisserant
authors.forename: Ali
authors.initials: A
authors.lastname: Siah
authors.forename: Florent
authors.initials: F
authors.lastname: Duyme
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Leducq
authors.forename: Caroline
authors.initials: C
authors.lastname: Deweer
authors.forename: Céline
authors.initials: C
authors.lastname: Fichter-Roisin
authors.forename: Jean
authors.initials: J
authors.lastname: Sanssené
authors.forename: Roger
authors.initials: R
authors.lastname: Durand
authors.forename: Patrice
authors.initials: P
authors.lastname: Halama
authors.forename: Philippe
authors.initials: P
authors.lastname: Reignault
mesh_terms: D000199:Actins / Q000235:genetics; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005602:France; D015966:Gene Expression Regulation, Fungal; D051456:Gene Flow; D005800:Genes, Fungal; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D058974:Phylogeography; D018807:Polymorphism, Single-Stranded Conformational; D004718:Saccharomycetales / Q000235:genetics* / Q000302:isolation & purification; D014404:Tubulin / Q000235:genetics
chemical_list: D000199:Actins; D004271:DNA, Fungal; D014404:Tubulin