title: Two forms of ribosomal protein L2 of Escherichia coli that inhibit DnaA in DNA replication.
abstract: We purified an inhibitor of oriC plasmid replication and determined that it is a truncated form of ribosomal protein L2 evidently lacking 59 amino acid residues from the C-terminal region encoded by rplB. We show that this truncated form of L2 or mature L2 physically interacts with the N-terminal region of DnaA to inhibit initiation from oriC by apparently interfering with DnaA oligomer formation, and the subsequent assembly of the prepriming complex on an oriC plasmid. Both forms of L2 also inhibit the unwinding of oriC by DnaA. These in vitro results raise the possibility that one or both forms of L2 modulate DnaA function in vivo to regulate the frequency of initiation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824-1319, USA.
authors.forename: Sundari
authors.initials: S
authors.lastname: Chodavarapu
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Felczak
authors.forename: Jon M
authors.initials: JM
authors.lastname: Kaguni
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000096:biosynthesis; D018741:Replication Origin; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D012269:Ribosomal Proteins; C029466:ribosomal protein L2; C560088:ribosomal protein L2, E coli; D000255:Adenosine Triphosphate