title: Lactoferrin inhibits the growth of nasal polyp fibroblasts.
abstract: The aim of this study was to evaluate the effects of lactoferrin (LF) on the growth of fibroblasts derived from nasal polyps. We showed that the proliferation of fibroblasts was inhibited in a dose-dependent manner by both native and recombinant LF. The greatest inhibition of proliferation was caused by human milk-derived, iron-saturated LF. The inhibition of fibroblast proliferation was not species specific because bovine LF also was active. The interaction between LFs and a putative cell receptor did not depend on the sugar composition of the glycan moiety of the LF molecule because lactoferrins of different origins were active and the addition of monosaccharides to the cultures did not block proliferation. However, the treatment of fibroblasts with sodium chlorate (an inhibitor of glycosaminoglycan sulfation) or the addition of heparin abolished the inhibitory effect of LF, suggesting that LF binds heparan sulfate-containing proteoglycans. The significance of LF in nasal excretions in controlling polyp formation is discussed.
authors.affiliation: Department of Otolaryngology, Head & Neck Surgery, Wroclaw Medical University, Borowska 213, PL 50-556 Wrocław, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Nadolska
authors.forename: Marcin
authors.initials: M
authors.lastname: Frączek
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kręcicki
authors.forename: Maja
authors.initials: M
authors.lastname: Kocięba
authors.forename: Michał
authors.initials: M
authors.lastname: Zimecki
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002704:Chlorates / Q000494:pharmacology; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology*; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism* / Q000494:pharmacology*; D008297:Male; D008892:Milk; D009298:Nasal Polyps; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D002704:Chlorates; D005343:Fibrinolytic Agents; D019812:Heparan Sulfate Proteoglycans; D011994:Recombinant Proteins; D006493:Heparin; D007501:Iron; D007781:Lactoferrin; C032706:sodium chlorate