title: On-line microdialysis system with poly(amidoamine)-encapsulated Pt nanoparticles biosensor for glutamate sensing in vivo.
abstract: In this work, an amine-terminated poly (amidoamine) dendrimer containing Pt nanoparticles (PAMAM/Pt) nanocomposite was synthesized and a novel amperometric H(2)O(2) biosensor based on PAMAM/Pt and MWCNTs was developed. The resulting film of MWCNTs/PAMAM/Pt was characterized by transmission electron microscopy (TEM), linear sweep voltammetry (LSV) and amperometric i-t curve. It demonstrates excellent electrocatalytic responses toward the reduction of H(2)O(2) at -200 mV (vs.SCE) without HRP participation. Immobilized with glutamate oxidase (GlutaOx), an effective glutamate biosensor, was fabricated, and the in vivo detection for glutamate was realized combining with the on-line microdialysis system. The glutamate biosensor showed good linear range from 1.0 μM to 50.0 μM with the detection limit of 0.5 μM (S/N=3). The basal level of glutamate in the striatum of rat was detected continuously with this on-line system and was calculated to be 5.80±0.12 μM (n=3). This method was proved to be sensitive and selective and may be feasible in the further application of physiology and pathology.
authors.affiliation: Department of Chemistry and Shanghai Key Laboratory of Green Chemistry and Chemical Process, East China Normal University, 3663 Zhongshan Road(N), Shanghai, 200062, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001923:Brain Chemistry; D050091:Dendrimers / Q000737:chemistry*; D004553:Electric Conductivity; D004563:Electrochemistry; D018698:Glutamic Acid / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles; D010984:Platinum / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D018698:Glutamic Acid; D010984:Platinum; D006861:Hydrogen Peroxide