title: [Evaluation of the SD BIOLINE TB Ag MPT64 Rapid® for the diagnosis of tuberculosis].
abstract: The purpose of this study was to evaluate the SD Bioline Ag MPT64 Rapid(®) for identification of the Mycobacterium tuberculosis complex. The method uses an immunochromatographic assay and needs 100 μl of sample taken from liquid culture or colonies suspended. The sensitivity was determined using 99 strains of M. tuberculosis complex and the specificity using 10 nontuberculous mycobacteria and 85 strains other than mycobacteria genus. The test showed excellent sensitivity (99%) and specificity (100%). This technique displays several advantages and is destined to spread in all laboratories and particularly in endemic areas.
authors.affiliation: Service de biologie médicale, HIA Percy, 101, avenue H.-Barbusse, 92141 Clamart, France. biologie.percy@orange-business.fr
authors.forename: M
authors.initials: M
authors.lastname: Fabre
authors.forename: R
authors.initials: R
authors.lastname: Vong
authors.forename: T
authors.initials: T
authors.lastname: Gaillard
authors.forename: A
authors.initials: A
authors.lastname: Merens
authors.forename: P
authors.initials: P
authors.lastname: Gérome
authors.forename: P
authors.initials: P
authors.lastname: Saint-Blancard
authors.forename: F
authors.initials: F
authors.lastname: Mechaï
authors.forename: F
authors.initials: F
authors.lastname: Janvier
authors.forename: F
authors.initials: F
authors.lastname: Nouridjan
authors.forename: C
authors.initials: C
authors.lastname: Soler
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D055910:Antibodies, Immobilized; D000942:Antigens, Bacterial / Q000032:analysis* / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D002845:Chromatography / Q000379:methods*; D004755:Enterobacteriaceae / Q000276:immunology; D005189:False Positive Reactions; D005260:Female; D006094:Gram-Positive Bacteria / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D066298:In Vitro Techniques; D008297:Male; D009154:Mutation; D009161:Mycobacterium / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013535:Suspensions; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080865:MPB64 protein, Mycobacterium; C510537:MPT64 protein, Mycobacterium tuberculosis; D013535:Suspensions