title: Methylation pattern of IFN-γ and IL-10 genes in periodontal tissues.
abstract: DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands. Unmethylated CpGs are related to transcriptionally active structure, whereas methylated CpG recruits methyl-binding proteins that promote chromatin compaction. DNA methylation can influence the expression of cytokines and affect the development of periodontal disease.
  
  
  OBJECTIVE
  The purpose of the present study was to evaluate the methylation status of the interferon gamma (IFN-γ) and interleukin-10 (IL-10) genes in periodontal tissues.
  
  
  METHODS
  Methylation-specific polymerase chain reaction (MSP) and DNA sequencing analysis were used to verify the DNA methylation status of the IFN-γ and IL-10 genes, respectively, in samples from subjects without periodontitis and individuals with chronic periodontitis. Histological sections stained by hematoxylin-eosin were used for histopathological evaluation of samples.
  
  
  RESULTS
  The methylation status of the IFN-γ and IL-10 genes was similar among the groups. Most of the samples were positive for IFN-γ methylation. Only 11% of the periodontitis group showed unmethylated DNA. Considering the IL-10 gene, no unmethylated sample was observed. The profile of total or partial methylation was detected in CpGs evaluated.
  
  
  CONCLUSIONS
  The results showed evidence that methylation of IFN-γ and IL-10 genes is a usual feature on periodontal tissues. Further studies are needed to determine the functional relevance of these alterations.
authors.affiliation: Laboratory of Molecular Biology, Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Michelle Beatriz
authors.initials: MB
authors.lastname: Viana
authors.forename: Fabiano Pereira
authors.initials: FP
authors.lastname: Cardoso
authors.forename: Marina Gonçalves
authors.initials: MG
authors.lastname: Diniz
authors.forename: Fernando Oliveira
authors.initials: FO
authors.lastname: Costa
authors.forename: José Eustáquio
authors.initials: JE
authors.lastname: da Costa
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
authors.forename: Paula Rocha
authors.initials: PR
authors.lastname: Moreira
mesh_terms: D001483:Base Sequence; D016022:Case-Control Studies; D002843:Chromatin / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D055113:Chronic Periodontitis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018899:CpG Islands / Q000276:immunology; D003596:Cytosine / Q000378:metabolism; D019175:DNA Methylation / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000276:immunology; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics* / Q000276:immunology
chemical_list: D002843:Chromatin; D016753:Interleukin-10; D007371:Interferon-gamma; D003596:Cytosine