title: Predicted expansion of the claudin multigene family.
abstract: Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members.
authors.affiliation: Graduate School of Information Science and Technology, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Mineta
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Hiroo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yukiyo
authors.initials: Y
authors.lastname: Tada
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Saito
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tamura
authors.forename: Michihiro
authors.initials: M
authors.lastname: Igarashi
authors.forename: Toshinori
authors.initials: T
authors.lastname: Endo
authors.forename: Kosei
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tsukita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D057167:Claudins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D030562:Databases, Protein; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D010802:Phylogeny; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins; D016415:Sequence Alignment; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D057167:Claudins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011994:Recombinant Proteins