title: Homogenized tissue phantoms for quantitative evaluation of subsurface fluorescence contrast.
abstract: The use of phantoms comprising diluted tissue homogenates with a buried capillary containing quantum dots is demonstrated as a method to investigate the optical and biophysical factors influencing the imaging of subsurface fluorescence contrast agents. Validation of the method is demonstrated using both liquid phantoms of known optical absorption and reduced scattering and Monte Carlo computer simulations of photon transport. Conclusions regarding the optimal excitation wavelength are given and quantified with respect to the tissue optical properties. The tissue homogenate method should be of value for quantitative optimization studies relevant to, for example, endoscopic imaging.
authors.affiliation: University Health Network, University of Toronto and Ontario Cancer Institute, Department of Medical Biophysics, 610 University Avenue, Toronto, ON, M5G 2M9 Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Roy
authors.forename: Anthony
authors.initials: A
authors.lastname: Kim
authors.forename: Farhan
authors.initials: F
authors.lastname: Dadani
authors.forename: Brian C
authors.initials: BC
authors.lastname: Wilson
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D010363:Pattern Recognition, Automated / Q000379:methods*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity