title: [Expression of ghrelin and its receptor GHS-R in the hypothalamus and gastrointestinal tract in rats with chronic renal failure].
abstract: OBJECTIVE
  To investigate the expression of ghrelin and its receptor, growth hormone secretagogue receptor (GHS-R), in the hypothalamus and gastrointestinal tract in rats with chronic renal failure (CRF) and explore their relationship with the disorder of gastrointestinal tract motility.
  
  
  METHODS
  SD rats were randomly divided into sham-operated group (n=8) and CRF group (n=16), and in the latter group, the rats were subjected to 5/6 nephrectomy to induce CRF. Real-time PCR and immunohistochemical staining were used to detect the distribution of mRNA and protein of ghrelin and GHS-R in the gastric fundus, duodenum, and hypothalamus.
  
  
  RESULTS
  The rats in the CRF group showed a significantly higher expression of ghrelin mRNA and protein in the gastric fundus but a lower expression in the hypothalamus than those in the sham-operated group (P<0.01), but the expression in the duodenum was similar between the two groups (P>0.05). The expression of GHS-R mRNA and protein in the gastric fundus was significantly higher in the CRF group than in the sham-operated group (P<0.01), while in the hypothalamus and duodenum, the expression was significantly lower in the CRF group (P<0.01).
  
  
  CONCLUSIONS
  The different distribution patterns of ghrelin and GHS-R in the tissues may be an important pathological basis of gastrointestinal motility disorder in CRF.
authors.affiliation: Department of Nephrology, Xi an Jiaotong University College of Medicine, Xi an 710004, China.
authors.forename: Rong-guo
authors.initials: RG
authors.lastname: Fu
authors.forename: Hao-zheng
authors.initials: HZ
authors.lastname: Yuan
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Heng
authors.initials: H
authors.lastname: Ge
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Qi-lan
authors.initials: QL
authors.lastname: Ning
authors.forename: Fu-jun
authors.initials: FJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000378:metabolism*; D054439:Ghrelin / Q000235:genetics / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D054440:Receptors, Ghrelin / Q000235:genetics / Q000378:metabolism*
chemical_list: D054439:Ghrelin; D012333:RNA, Messenger; D054440:Receptors, Ghrelin