title: Anti-inflammatory activity of Angelica dahurica ethanolic extract on RAW264.7 cells via upregulation of heme oxygenase-1.
abstract: In this study, we analyzed the anti-inflammatory effects of Angelica dahurica Bentham et Hooker ethanolic extract (ADEE) on RAW264.7 cells, to understand the mechanism underlying its observed effects. ADEE inhibited cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to the suppression of COX-2-derived prostaglandin E(2) and iNOS-derived production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. These inhibitory effects of ADEE were accompanied by the reduced production of tumor necrosis factor α and interleukin (IL)-6. ADEE also inhibited nuclear factor κB (NFκB) translocation to the nucleus by interrupting inhibitor kappa Bα (IκBα) degradation. ADEE upregulated heme oxygenase 1 expression, and treatment with tin protoporphyrin IX (SnPP), a selective inhibitor of HO-1, reversed the LPS-induced generation of proinflammatory cytokines. ADEE also induced IL-4 and IL-5 expression in concanavalin-A-stimulated splenocytes. These results suggest that ADEE has anti-inflammatory activity, which acts via the suppression of the NF-κB pathway.
authors.affiliation: Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Exporo 483, Yusung-gu, Daejeon 305-811, Republic of Korea.
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Jin-Ah
authors.initials: JA
authors.lastname: Lee
authors.forename: Chang-Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Heykyung
authors.initials: H
authors.lastname: Ha
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Jong-Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Hyeun-Kyoo
authors.initials: HK
authors.lastname: Shin
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D020672:I-kappa B Proteins / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008665:Metalloporphyrins / Q000378:metabolism; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011524:Protoporphyrins / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism; D015854:Up-Regulation; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D051546:Cyclooxygenase 2; D016207:Cytokines; D051547:Heme Oxygenase-1; D020672:I-kappa B Proteins; D015847:Interleukin-4; D015848:Interleukin-5; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D008665:Metalloporphyrins; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts; D011524:Protoporphyrins; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors; C000606205:Nfkbia protein, mouse; C032628:tin protoporphyrin IX; C496317:Nos2 protein, mouse; C496542:Hmox1 protein, mouse; C077623:Ptgs2 protein, mouse