title: A novel sequence-based coa genotyping method to discriminate nosocomial methicillin-resistant Staphylococcus aureus isolates.
abstract: OBJECTIVE
  To evaluate a novel sequence-based coa typing method in classification for nosocomial methicillin-resistant Staphylococcus aureus.
  
  
  METHODS
  D1 and D2 regions of coa gene were used to discriminate methicillin-resistant Staphylococcus aureus and compared with other classical sequence-based methods with discriminate power.
  
  
  RESULTS
  All MRSA isolates were divided into 11 types and about 20 subtypes with coa typing method here. The discriminate power of this coa typing method was better than MLST or spa method.
  
  
  CONCLUSIONS
  It was demonstrated that this coa method is a simple, rapid, and effective technique applicable for outbreak or local nosocomial MRSA investigations in the future.
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Q T
authors.initials: QT
authors.lastname: Li
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Zhu
authors.forename: K
authors.initials: K
authors.lastname: Dong
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhou
authors.forename: Y X
authors.initials: YX
authors.lastname: Ni
authors.forename: X K
authors.initials: XK
authors.lastname: Guo
mesh_terms: D015373:Bacterial Typing Techniques; D003030:Coagulase / Q000235:genetics*; D003428:Cross Infection / Q000382:microbiology*; D052978:Disk Diffusion Antimicrobial Tests; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D013203:Staphylococcal Infections / Q000235:genetics*
chemical_list: D003030:Coagulase