title: Cisplatin-loaded gelatin-poly(acrylic acid) nanoparticles: synthesis, antitumor efficiency in vivo and penetration in tumors.
abstract: Cisplatin (CDDP)-loaded gelatin-poly(acrylic acid) (GEL-PAA) nanoparticles were successfully prepared by polymerizing acrylic acid in the presence of gelatin in aqueous solution followed by incorporating CDDP into the formed GEL-PAA nanoparticles through polymer-metal complex formation of CDDP with carboxylic groups in the nanoparticles. The obtained nanoparticles had a spherical shape, with a mean size of about 100 nm, and high drug payload as well as stability. It is found that CDDP can be released from the nanoparticles in a sustained manner with a small initial burst release. In vitro cytotoxicity revealed that CDDP-loaded nanoparticles had similar cytotoxicity to free CDDP after 48 h co-incubation with human colorectal cancer cell line LoVo. In vivo antitumor activity indicated that the nanoparticle formulation was superior in anticancer effect to free CDDP on murine hepatic H22 tumor-bearing mice model through intraperitoneal (i.p.) administration and displayed a dose-dependent antitumor efficacy. Further, the penetration examination of the nanoparticles through tumor tissue revealed that the CDDP-loaded GEL-PAA nanoparticles could only affect the cells near the tumor vasculature after they entered into the tumor tissue.
authors.affiliation: Department of Polymer Science & Engineering, Nanjing University, Nanjing, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.forename: Zhenshu
authors.initials: Z
authors.lastname: Zhu
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Hu
authors.forename: Xiqun
authors.initials: X
authors.lastname: Jiang
authors.forename: Baorui
authors.initials: B
authors.lastname: Liu
mesh_terms: D000179:Acrylates / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D002945:Cisplatin / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004353:Drug Evaluation, Preclinical; D004355:Drug Stability; D005562:Formazans / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D000179:Acrylates; D000970:Antineoplastic Agents; D004337:Drug Carriers; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; D005780:Gelatin; C036658:acrylic acid; D002945:Cisplatin