title: 1H, 13C and 15N backbone and side-chain chemical shift assignment of the Fyn SH2 domain and its complex with a phosphotyrosine peptide.
abstract: SH2 domains are interaction modules uniquely dedicated to recognize phosphotyrosine sites, playing a central role in for instance the activation of tyrosine kinases or phosphatases. Here we report the (1)H, (15)N and (13)C backbone and side-chain chemical shift assignments of the SH2 domain of the human protein tyrosine kinase Fyn, both in its free state and bound to a high-affinity phosphotyrosine peptide corresponding to a specific sequence in the hamster middle-T antigen. The BMRB accession numbers are 17,368 and 17,369, respectively.
authors.affiliation: Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussel, Belgium.
authors.forename: Radu
authors.initials: R
authors.lastname: Huculeci
authors.forename: Lieven
authors.initials: L
authors.lastname: Buts
authors.forename: Tom
authors.initials: T
authors.lastname: Lenaerts
authors.forename: Nico A J
authors.initials: NA
authors.lastname: van Nuland
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000737:chemistry; D006224:Cricetinae; D006801:Humans; D007554:Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D019000:Phosphotyrosine / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D051076:Proto-Oncogene Proteins c-fyn / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D018909:src Homology Domains
chemical_list: D000957:Antigens, Viral, Tumor; D007554:Isotopes; D010455:Peptides; D011994:Recombinant Proteins; D019000:Phosphotyrosine; C494957:FYN protein, human; D051076:Proto-Oncogene Proteins c-fyn