title: Proteomic analysis of cell lines to identify the irinotecan resistance proteins.
abstract: Chemotherapeutic drug resistance is a frequent cause of treatment failure in colon cancer patients. Several mechanisms have been implicated in drug resistance. However, they are not sufficient to exhaustively account for this resistance emergence. In this study, two-dimensional gel electrophoresis (2-DE) and the PDQuest software analysis were applied to compare the differential expression of irinotecan-resistance-associated protein in human colon adenocarcinoma LoVo cells and irinotecan-resistant LoVo cells (LoVo/irinotecan). The differential protein dots were excised and analysed by ESI-Q-TOF mass spectrometry (MS). Fifteen proteins were identified, including eight proteins with decreased expression and seven proteins with increased expression. The identified known proteins included those that function in diverse biological processes such as cellular transcription, cell apoptosis, electron transport/redox regulation, cell proliferation/differentiation and retinol metabolism pathways. Identification of such proteins could allow improved understanding of the mechanisms leading to the acquisition of chemoresistance.
authors.affiliation: Department of Medical Oncology, Cancer Center, and The State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, No. 37, Guo Xue Xiang, Chengdu 610041, Sichuan Province, China.
authors.forename: Xing-Chen
authors.initials: XC
authors.lastname: Peng
authors.forename: Feng-Ming
authors.initials: FM
authors.lastname: Gong
authors.forename: Meng
authors.initials: M
authors.lastname: Wei
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Cheng
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Liu
mesh_terms: D000449:Aldehyde Reductase / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D006801:Humans; D000077146:Irinotecan; D008969:Molecular Sequence Data; D011355:Prodrugs / Q000494:pharmacology*; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D000970:Antineoplastic Agents; D011355:Prodrugs; D020543:Proteome; D000077146:Irinotecan; D000449:Aldehyde Reductase; D002166:Camptothecin