title: Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency.
abstract: We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.
authors.affiliation: Department of Biology (Area 5), University of York, Wentworth Way, York YO10 5DD, United Kingdom.
authors.forename: Alison D
authors.initials: AD
authors.lastname: Walters
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Smith
authors.forename: James P J
authors.initials: JP
authors.lastname: Chong
mesh_terms: D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D017017:Methanococcus / Q000235:genetics*; D014169:Transformation, Bacterial