title: Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica.
abstract: Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral genome sequences. In this study, viral DNA fragments were obtained using RDV, and approximately 15-kbp circular full genome sequences were determined using a next-generation sequencing system, overlapping PCR, and Southern blot analysis. One open reading frame (ORF) was homologous to the large T-antigen of polyomavirus; other ORFs have no homology with any nucleic or amino acid sequences of polyomavirus. Therefore, as this DNA virus might comprise a novel virus family, we provisionally named it Japanese eel endothelial cells-infecting virus (JEECV). JEECV was detected in both naturally and experimentally infected eels, suggesting that JEECV potentially causes VECNE.
authors.affiliation: Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo, Japan. tmizutan@nih.go.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sayama
authors.forename: Akira
authors.initials: A
authors.lastname: Nakanishi
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ochiai
authors.forename: Kouji
authors.initials: K
authors.lastname: Sakai
authors.forename: Kouji
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Nozomi
authors.initials: N
authors.lastname: Tanaka
authors.forename: Emi
authors.initials: E
authors.lastname: Miura
authors.forename: Mami
authors.initials: M
authors.lastname: Oba
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ono
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla / Q000821:virology*; D000818:Animals; D015139:Blotting, Southern; D016000:Cluster Analysis; D004267:DNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D042783:Endothelial Cells / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins