title: Modification of an in vitro model simulating the whole digestive process to investigate cellular endpoints of chemoprevention.
abstract: In vitro gut fermentation systems are relevant tools to study health benefits of foodstuffs. Most of them are commonly used to investigate the degradation of nutrients or the development of gut flora. Using these models, strong cytotoxic effects of the resulting samples on cultured cells were observed. Hence, the aim of the present study was to develop a modified in vitro fermentation model that simulates the whole digestive tract and generates fermented samples that are suitable for testing in cell culture experiments. Wholemeal wheat flour (wwf) was digested and fermented in vitro with a fermentation model using different ox gall concentrations (41·6 and 0·6 g/l). The resulting fermentation supernatants (fs) were characterised for metabolites and biological effects in HT29 cells. The fermentation of wwf increased chemopreventive SCFA and decreased carcinogenic deoxycholic acid (DCA). The strong cytotoxic effects of the fs, which were partly due to cholic acid and DCA, were diminished by lowering the ox gall concentration, allowing the use of the samples in cell culture experiments. In conclusion, an in vitro digestion model, which can be used to study the effects of foodstuffs on chemoprevention and gut health in colon cells, is introduced and its physiological relevance is demonstrated.
authors.affiliation: Department of Nutritional Toxicology, Institute for Nutrition, Friedrich-Schiller-University Jena, Dornburger Strasse 24, Jena, Germany. stein.katrin@googlemail.com
authors.forename: Katrin
authors.initials: K
authors.lastname: Stein
authors.forename: Anke
authors.initials: A
authors.lastname: Borowicki
authors.forename: Daniel
authors.initials: D
authors.lastname: Scharlau
authors.forename: Kerstin
authors.initials: K
authors.lastname: Scheu
authors.forename: Gerald
authors.initials: G
authors.lastname: Brenner-Weiss
authors.forename: Ursula
authors.initials: U
authors.lastname: Obst
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hollmann
authors.forename: Meinolf
authors.initials: M
authors.lastname: Lindhauer
authors.forename: Norbert
authors.initials: N
authors.lastname: Wachter
authors.forename: Michael
authors.initials: M
authors.lastname: Glei
mesh_terms: D000230:Adenocarcinoma / Q000517:prevention & control; D000818:Animals; D001646:Bile / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D045744:Cell Line, Tumor; D002793:Cholic Acids / Q000009:adverse effects / Q000378:metabolism* / Q000494:pharmacology; D003106:Colon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000517:prevention & control*; D004063:Digestion; D002523:Edible Grain / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D005285:Fermentation; D005433:Flour; D006801:Humans; D008954:Models, Biological; D014908:Triticum
chemical_list: D002793:Cholic Acids; D005232:Fatty Acids, Volatile