title: Antinociceptive activity of Melicope ptelefolia ethanolic extract in experimental animals.
abstract: Melicope ptelefolia is a medicinal herb commonly used in Malaysia to treat fever, pain, wounds, and itches. The present study was conducted to evaluate the antinociceptive activity of the Melicope ptelefolia ethanolic extract (MPEE) using animal models of nociception. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin-induced paw licking tests. Oral administration of MPEE produced significant dose-dependent antinociceptive effects when tested in mice and rats using acetic acid-induced abdominal constriction test and on the second phase of the formalin-induced paw licking test, respectively. It was also demonstrated that MPEE had no effect on the response latency time to the heat stimulus in the thermal model of the hot-plate test. In addition, the antinociception produced by MPEE was not blocked by naloxone. Furthermore, oral administration of MPEE did not produce any effect in motor performance of the rota-rod test and in acute toxicity study no abnormal behaviors as well as mortality were observed up to a dose level of the extract of 5 g/kg. These results indicated that MPEE at all doses investigated which did not produce any sedative and toxic effects exerted pronounce antinociceptive activity that acts peripherally in experimental animals.
authors.affiliation: Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia. mrs@medic.upm.edu.my
authors.forename: Mohd Roslan
authors.initials: MR
authors.lastname: Sulaiman
authors.forename: Azyyati
authors.initials: A
authors.lastname: Mohd Padzil
authors.forename: Khozirah
authors.initials: K
authors.lastname: Shaari
authors.forename: Syamimi
authors.initials: S
authors.lastname: Khalid
authors.forename: Wan Mastura
authors.initials: WM
authors.lastname: Shaik Mossadeq
authors.forename: Azam Shah
authors.initials: AS
authors.lastname: Mohamad
authors.forename: Syahida
authors.initials: S
authors.lastname: Ahmad
authors.forename: Ahmad
authors.initials: A
authors.lastname: Akira
authors.forename: Daud
authors.initials: D
authors.lastname: Israf
authors.forename: Nordin
authors.initials: N
authors.lastname: Lajis
mesh_terms: D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009048:Motor Skills / Q000187:drug effects; D009619:Nociceptors / Q000187:drug effects; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027821:Rutaceae / Q000737:chemistry*; D023382:Toxicity Tests, Acute
chemical_list: D010936:Plant Extracts