title: Altered expression of middle and inner ear cytokines in mouse otitis media.
abstract: OBJECTIVE
  The inner ear is at risk for sensorineural hearing loss in both acute and chronic otitis media (OM), but the mechanisms underlying sensorineural hearing loss are unknown. Previous gene expression array studies have shown that cytokine genes might be upregulated in the cochleas of mice with acute and chronic OM. This finding implies that the inner ear could manifest a direct inflammatory response to OM that may cause sensorineural damage. Therefore, to better understand inner ear cytokine gene expression during OM, quantitative real-time polymerase chain reaction and immunohistochemistry were used in mouse models to evaluate middle and inner ear inflammatory and remodeling cytokines.
  
  
  METHODS
  Basic science experiment.
  
  
  METHODS
  An acute OM model was created in Balb/c mice by a transtympanic injection of Streptococcus pneumoniae in one ear; the other ear was used as a control. C3H/HeJ mice were screened for unilateral chronic OM, with the noninfected ear serving as a control.
  
  
  RESULTS
  Both acute and chronic OM caused both the middle ear and inner tissues in these two mouse models to overexpress numerous cytokine genes related to tissue remodeling (tumor necrosis factor-α, bone morphogenetic proteins, fibroblast growth factors) and angiogenesis (vascular endothelial growth factor), as well as inflammatory cell proliferation (interleukin [IL]-1α,β, IL-2, IL-6). Immunohistochemistry confirmed that both the middle ear and inner ear tissues expressed these cytokines.
  
  
  CONCLUSIONS
  Cochlear tissues are capable of expressing cytokine mRNA that contributes to the inflammation and remodeling that occur in association with middle ear disease. This provides a potential molecular basis for the transient and permanent sensorineural hearing loss often reported with acute and chronic OM.
authors.affiliation: Department of Otolaryngology, Oregon Hearing Research Center, Oregon Health and Science University, Portland, Oregon 97239-3098, USA. macarthc@ohsu.edu
authors.forename: Carol J
authors.initials: CJ
authors.lastname: MacArthur
authors.forename: De-Ann M
authors.initials: DA
authors.lastname: Pillers
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Pang
authors.forename: J Beth
authors.initials: JB
authors.lastname: Kempton
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Trune
mesh_terms: D000208:Acute Disease; D000818:Animals; D002908:Chronic Disease; D016207:Cytokines / Q000032:analysis*; D007758:Ear, Inner / Q000737:chemistry*; D004432:Ear, Middle / Q000737:chemistry*; D015870:Gene Expression; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010033:Otitis Media / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D016207:Cytokines; D012333:RNA, Messenger