title: [Establishment of B lymphoblastoid cell lines of Miao pedigree with Bardet-Biedl syndrome].
abstract: OBJECTIVE
  To establish immortalized lymphoblastoid cell lines of a Miao core pedigree with Bardet-Biedl syndrome (BBS), in order to provide a long-term source of material for research.
  
  
  METHODS
  With Epstein-Barr virus transformation of B cells and addition of cyclosporine A to inhibit the activity of T cells, fresh anticoagulated blood samples with heparin were collected from 12 members of the core pedigree, and were used to establish the immortalized lymphoblastoid cell lines of B lymphocytes.
  
  
  RESULTS
  Twelve immortalized lymphoblastoid cell lines of the core BBS pedigree were obtained successfully.
  
  
  CONCLUSIONS
  The immortalized B lymphoblastoid cell lines of the Miao pedigree with BBS can preserve the whole genome information and provide long-term research materials for BBS study.
authors.affiliation: Institute of Basic Medicine, the First People's Hospital of Yunnan Province, Kunhua Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, 650032 PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
authors.forename: Tao
authors.initials: T
authors.lastname: Shou
authors.forename: Ke-qin
authors.initials: KQ
authors.lastname: Lin
authors.forename: Wen
authors.initials: W
authors.lastname: Yi
authors.forename: Ying-kun
authors.initials: YK
authors.lastname: Hua
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.forename: Jian-mei
authors.initials: JM
authors.lastname: Gao
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Lin-ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Jin-li
authors.initials: JL
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xin-min
authors.initials: XM
authors.lastname: Yan
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology*; D020788:Bardet-Biedl Syndrome / Q000097:blood* / Q000235:genetics*; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002681:China / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics*; D004854:Herpesvirus 4, Human; D006801:Humans; D010375:Pedigree