title: Salinivibrio sharmensis sp. nov., a novel haloalkaliphilic bacterium from a saline lake in Ras Mohammed Park (Egypt).
abstract: A novel haloalkaliphilic, facultative anaerobic and Gram-negative Salinivibrio-like microorganism (designated strain BAG(T)) was recovered from a saline lake in Ras Mohammed Park (Egypt). Cells were motile, curved rods, not spore-forming and occurred singly. Strain BAG(T) grew optimally at 35°C (temperature growth range 25-40°C) with 10.0% (w/v) NaCl [NaCl growth range 6.0-16.0% (w/v)] and at pH 9.0 (pH growth range 6.0-10.0). Strain BAG(T) had phosphatidylethanolamine (PEA) and phosphatidylglycerol (PG) as the main polar lipids, C16:0 (54.0%) and C16:1 (26.0%) as the predominant cellular fatty acids and Q-8 as the major respiratory quinone. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BAG(T) was a member of Salinivibrio genus, with the highest sequence similarities of 99.1, 98.4 and 98.1% to Salinivibrio siamensis JCM 14472(T), Salinivibrio proteolyticus DSM 19052(T) and Salinivibrio costicola subsp. alcaliphilus DSM 16359(T), respectively. DNA-DNA hybridization values of strain BAG(T) with members of Salinivibrio genus were lower than 55.0%. DNA G + C content was 51.0 mol%. On the basis of the polyphasic taxonomic results revealed in this study, strain BAG(T) should be classified as a novel species of Salinivibrio genus, for which the name Salinivibrio sharmensis sp. nov. is proposed, with the type strain BAG(T) (=ATCC BAA-1319(T) = DSM 18182(T)).
authors.affiliation: Istituto di Chimica Biomolecolare, CNR, via Campi Flegrei n. 34, 80078 Pozzuoli (Naples), Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Romano
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Orlando
authors.forename: Agata
authors.initials: A
authors.lastname: Gambacorta
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicolaus
authors.forename: Laura
authors.initials: L
authors.lastname: Dipasquale
authors.forename: Javier
authors.initials: J
authors.lastname: Pascual
authors.forename: Assunta
authors.initials: A
authors.lastname: Giordano
authors.forename: Licia
authors.initials: L
authors.lastname: Lama
mesh_terms: D002241:Carbohydrates / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature; D014737:Vibrionaceae / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D002241:Carbohydrates; D008055:Lipids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine; D012965:Sodium Chloride; D004247:DNA