title: Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma.
abstract: We established a novel feline B-cell line, MS4, from the neoplastic pleural effusion of a cat with cutaneous B-cell lymphoma. Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nishigaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ide
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sato
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sato
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kotera
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D001483:Base Sequence; D002371:Cat Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology*; D002415:Cats; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D016130:Immunophenotyping; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000302:isolation & purification*; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D008297:Male
chemical_list: D004279:DNA, Viral