title: Expression of the human atypical kinase ADCK3 rescues coenzyme Q biosynthesis and phosphorylation of Coq polypeptides in yeast coq8 mutants.
abstract: Coenzyme Q (ubiquinone or Q) is a lipid electron and proton carrier in the electron transport chain. In yeast Saccharomyces cerevisiae eleven genes, designated COQ1 through COQ9, YAH1 and ARH1, have been identified as being required for Q biosynthesis. One of these genes, COQ8 (ABC1), encodes an atypical protein kinase, containing six (I, II, III, VIB, VII, and VIII) of the twelve motifs characteristically present in canonical protein kinases. Here we characterize seven distinct Q-less coq8 yeast mutants and show that unlike the coq8 null mutant, each maintained normal steady-state levels of the Coq8 polypeptide. The phosphorylation states of Coq polypeptides were determined with two-dimensional gel analyses. Coq3p, Coq5p, and Coq7p were phosphorylated in a Coq8p-dependent manner. Expression of a human homolog of Coq8p, ADCK3(CABC1) bearing an amino-terminal yeast mitochondrial leader sequence, rescued growth of yeast coq8 mutants on medium containing a nonfermentable carbon source and partially restored biosynthesis of Q(6). The phosphorylation state of several of the yeast Coq polypeptides was also rescued, indicating a profound conservation of yeast Coq8p and human ADCK3 protein kinase function in Q biosynthesis.
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angles, Los Angeles, CA 90095-1569, USA.
authors.forename: Letian X
authors.initials: LX
authors.lastname: Xie
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Hsieh
authors.forename: Shota
authors.initials: S
authors.lastname: Watanabe
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Allan
authors.forename: Jia Y
authors.initials: JY
authors.lastname: Chen
authors.forename: UyenPhuong C
authors.initials: UC
authors.lastname: Tran
authors.forename: Catherine F
authors.initials: CF
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014451:Ubiquinone / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: C099891:COQ8 protein, S cerevisiae; C553453:COQ8A protein, human; D024101:Mitochondrial Proteins; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D014451:Ubiquinone; C030778:ubiquinone 8