title: In situ antibody detection and charge discrimination using aqueous stable pentacene transistor biosensors.
abstract: Pentacene-based organic thin-film transistors were used to create highly sensitive, real-time electronic sensors for selective antibody detection. Bovine serum albumin was covalently attached to a modified pentacene surface to selectively detect the label free monoclonal antiBSA. These sensors displayed a high affinity constant (K(A)) of (1.1 ± 3) × 10(7) M(-1) at pH 7, which is 1 order of magnitude higher than those obtained with a highly sensitive surface plasmon resonance spectroscopy detection system. Furthermore, a high degree of discrimination in the hybrid antiBSA charges was achieved at different pH values. This demonstration of fast, label-free, real-time detection of nanoscale biomolecules in aqueous buffer solutions using the organic transistor sensing platform will have a significant impact on high-performance microarrays in addition to discriminating the presence of ionizable groups.
authors.affiliation: Material Science Group, Max-Planck Institute for Polymer Research, Ackermannweg-10, D-55128 Mainz, Germany. khanh@mpip-mainz.mpg.de
authors.forename: Hadayat Ullah
authors.initials: HU
authors.lastname: Khan
authors.forename: Junhyuk
authors.initials: J
authors.lastname: Jang
authors.forename: Jang-Joo
authors.initials: JJ
authors.lastname: Kim
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knoll
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D004800:Enzymes, Immobilized; D017403:In Situ Hybridization; D015394:Molecular Structure; D009279:Naphthacenes / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D020349:Surface Plasmon Resonance; D014173:Transistors, Electronic; D014867:Water / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D009279:Naphthacenes; D014867:Water; D012710:Serum Albumin, Bovine; C523499:pentacene