title: Study on perfume stimulating olfaction with volatile oil of Acorus gramineus for treatment of the Alzheimer's disease rat.
abstract: OBJECTIVE
  To probe into the therapeutic effect of perfume stimulating olfaction with volatile oil of Acorus Gramineus on the Alzheimer's disease (AD) rat.
  
  
  METHODS
  Totally 50 adult SD rats, male,weighing 300 +/- 10 g, were randomly divided into 5 groups, normal group (group A), olfactory nerve severing model group (group B), AD model group (group C), AD model plus perfume stimulation group (group D), AD model olfactory nerve severing plus perfume stimulation group (group E), 10 rats in each group. After perfume stimulation, Morris maze test was conducted for valuating the learning and memory ability; Malondaldehyde (MDA) content, and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the brain, and the brain weight were detected.
  
  
  RESULTS
  Compared with the AD model group, the average escape latency and swimming distance in 6 days were significantly shorter than those in the group A, B, D (P < 0.01), with no significant differences between the group C and the group E (P > 0.05); Compared with the group A, B and D, MDA content in the group C significantly increased (P < 0.01), and SOD and GSH-Px activities significantly decreased (P < 0.01), and brain weight/body weight decreased significantly in the group C (P < 0.01), with no significant differences between the group C and the group E (P > 0.05).
  
  
  CONCLUSIONS
  Perfume stimultating olfaction with volatile oil of Acorus Gramineus can significantly increase the learning-memory ability, decrease MDA content and increase SOD and GSH-Px activities and weight of brain in AD rats.
authors.affiliation: Experimental Research Center, Department of Acupuncture and Tuina, Shaanxi College of Traditional Chinese Medicine, Xi'an 712046, China. lzb210396@163.com
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Liu
authors.forename: Wen-Min
authors.initials: WM
authors.lastname: Niu
authors.forename: Xiao-Hang
authors.initials: XH
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei-Gang
authors.initials: WG
authors.lastname: Wang
mesh_terms: D029023:Acorus / Q000737:chemistry*; D000544:Alzheimer Disease / Q000503:physiopathology / Q000523:psychology / Q000628:therapy*; D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007858:Learning; D008297:Male; D008568:Memory; D009822:Oils, Volatile / Q000627:therapeutic use*; D010476:Perfume / Q000627:therapeutic use*; D010938:Plant Oils / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012903:Smell / Q000187:drug effects*
chemical_list: D009822:Oils, Volatile; D010476:Perfume; D010938:Plant Oils