title: Spectroscopic analyses on interaction of o-Vanillin-D-Phenylalanine, o-Vanillin-L-Tyrosine and o-Vanillin-L-Levodopa Schiff Bases with bovine serum albumin (BSA).
abstract: In this work, three o-Vanillin Schiff Bases (o-VSB: o-Vanillin-D-Phenylalanine (o-VDP), o-Vanillin-L-Tyrosine (o-VLT) and o-Vanillin-L-Levodopa (o-VLL)) with alanine constituent were synthesized by direct reflux method in ethanol solution, and then were used to study the interaction to bovine serum albumin (BSA) molecules by fluorescence spectroscopy. Based on the fluorescence quenching calculation, the bimolecular quenching constant (K(q)), apparent quenching constant (K(sv)), effective binding constant (K(A)) and corresponding dissociation constant (K(D)) as well as binding site number (n) were obtained. In addition, the binding distance (r) was also calculated according to Foster's non-radioactive energy transfer theory. The results show that these three o-VSB can efficiently bind to BSA molecules, but the binding array order is o-VDP-BSA>o-VLT-BSA>o-VLL-BSA. Synchronous fluorescence spectroscopy indicates that the o-VDP is more accessibility to tryptophan (Trp) residues of BSA molecules than to tyrosine (Tyr) residues. Nevertheless, the o-VLT and o-VLL are more accessibility to Tyr residues than to Trp residues.
authors.affiliation: Department of Chemistry, Liaoning University, Shenyang, PR China.
authors.forename: Jingqun
authors.initials: J
authors.lastname: Gao
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Zhiqiu
authors.initials: Z
authors.lastname: Wang
authors.forename: Xudong
authors.initials: X
authors.lastname: Jin
authors.forename: Chunping
authors.initials: C
authors.lastname: Cheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Li
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000737:chemistry*; D002417:Cattle; D015259:Dopamine Agents / Q000737:chemistry; D007980:Levodopa / Q000737:chemistry*; D015394:Molecular Structure; D010649:Phenylalanine / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D015259:Dopamine Agents; D012545:Schiff Bases; C060843:2-vanillin; D012710:Serum Albumin, Bovine; D014443:Tyrosine; D007980:Levodopa; D010649:Phenylalanine