title: Conjugation reactions in the preparations of quantum dot-based immunoluminescent probes for analysis of proteins by capillary electrophoresis.
abstract: A number of biologically important molecules, such as DNA, proteins, and antibodies, are routinely conjugated with fluorescent tags for high-sensitivity analyses. Here, the application of quantum dots in the place of bright and size-tunable luminophores is studied. Several selected bioconjugation reactions via zero-length cross-linkers, long-chain linkers, and oriented methods for linking of quantum dots with proteins were tested. Anti-ovalbumin, anti-proliferating cell nuclear antigen, anti-hemagglutinin, and anti-CD3 membrane protein as model antibodies and annexin V were used as high-specificity selectors. The reaction yield and efficiency of the prepared immunoluminescent probes were tested by capillary zone electrophoresis with laser-induced fluorescence detection.
authors.affiliation: Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 602 00 Brno, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Lišková
authors.forename: I
authors.initials: I
authors.lastname: Voráčová
authors.forename: K
authors.initials: K
authors.lastname: Klepárník
authors.forename: V
authors.initials: V
authors.lastname: Hezinová
authors.forename: J
authors.initials: J
authors.lastname: Přikryl
authors.forename: F
authors.initials: F
authors.lastname: Foret
mesh_terms: D000906:Antibodies / Q000737:chemistry; D019075:Electrophoresis, Capillary; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry*; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D045663:Quantum Dots
chemical_list: D000906:Antibodies; D015335:Molecular Probes; D011506:Proteins