title: [Mechanisms of ultraviolet B irradiation-induced injuries in 16HBE cells].
abstract: OBJECTIVE
  To investigate the biological effects of ultraviolet B (UVB) irradiation on human bronchial epithelial cells (16HBE cells) and explore the possible mechanism.
  
  
  METHODS
  The survival rates of 16HBE cells were detected by MTT assay at 12 h after UVB irradiation at different doses (0, 10, 30, 50, 70, and 100 J/m(2)) or at 50 J/m(2) for different durations (2, 4, 8, 12, and 24 h). The DNA ladder was detected by agarose gel electrophoresis, the cell cycle changes were analyzed by flow cytometry, and the expression of nuclear factor-κB (NF-κB)/p65 protein was assayed by Western blotting following the exposures.
  
  
  RESULTS
  UVB irradiation of the cells resulted in lowered cell survival rates, DNA fragmentation, S phase arrest and up-regulation of NF-κB/p65 protein expression.
  
  
  CONCLUSIONS
  UVB irradiation can induce growth inhibition and apoptosis of 16HBE cells, in which process NF-κB protein may play a key role.
authors.affiliation: Department of Radiation Medicine, Southern Medical University, Guangzhou 510515, China. luchao1115@163.com
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Ding
authors.forename: Mei-juan
authors.initials: MJ
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D042783:Endothelial Cells / Q000166:cytology / Q000528:radiation effects*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D016328:NF-kappa B