title: Heterologous expression, biosynthesis, and mutagenesis of type II lantibiotics from Bacillus licheniformis in Escherichia coli.
abstract: Lichenicidin is a class II two-component lantibiotic produced by Bacillus licheniformis. It is composed of the two peptides Bliα and Bliβ, which act synergistically against various Gram-positive bacteria. The lichenicidin gene cluster was successfully expressed in Escherichia coli, thus constituting the first report to our knowledge of a full reconstitution of a lantibiotic biosynthetic pathway in vivo by a Gram-negative host. This system was further exploited to characterize and assign the function of proteins encoded in the biosynthetic gene cluster in the maturation of lichenicidin peptides. Moreover, a trans complementation system was developed for expression of Bliα and Bliβ variants in vivo. This contribution will spur future studies in the heterologous expression and engineering of lantibiotics.
authors.affiliation: Department of Biology and CESAM, University of Aveiro, 3810 Aveiro, Portugal; Medinfar-Pharmaceutical Products SA, Amadora, 2700 Venda Nova, Portugal.
authors.forename: Tânia
authors.initials: T
authors.lastname: Caetano
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Krawczyk
authors.forename: Eva
authors.initials: E
authors.lastname: Mösker
authors.forename: Roderich D
authors.initials: RD
authors.lastname: Süssmuth
authors.forename: Sónia
authors.initials: S
authors.lastname: Mendo
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D021381:Protein Transport
chemical_list: D001430:Bacteriocins; D010446:Peptide Fragments; C546394:lichenicidin, Bacillus licheniformis; D010447:Peptide Hydrolases