title: Peripheral blood galectin-1-expressing T and natural killer cells in normal pregnancy and preeclampsia.
abstract: The purpose of this study was to determine whether the proportion of galectin-1-expressing peripheral blood T and NK cells is altered in normal pregnancy and preeclampsia (PE). We also examined whether circulating levels of galectin-1 and anti-galectin-1 autoantibodies are affected in PE. Seventy preeclamptic patients, 75 healthy pregnant and 21 healthy non-pregnant women were involved in this study. Serum galectin-1 and anti-galectin-1 autoantibody levels were measured by ELISA. Intracellular galectin-1 expression of lymphocytes was determined with flow cytometry. Serum galectin-1 and anti-galectin-1 IgG levels did not differ significantly between the healthy pregnant and the PE group. In healthy pregnant women, significantly higher percentage of T and NK cells expressed gal-1 in their cytoplasma than in healthy non-pregnant women. However, the proportion of galectin-1-expressing peripheral blood T and NK cells was markedly decreased in PE compared to normal pregnancy, which might contribute to the activation of innate and acquired immune cells.
authors.affiliation: First Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary. molvarec@freemail.hu
authors.forename: Attila
authors.initials: A
authors.lastname: Molvarec
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Blois
authors.forename: Balázs
authors.initials: B
authors.lastname: Stenczer
authors.forename: Gergely
authors.initials: G
authors.lastname: Toldi
authors.forename: Irene
authors.initials: I
authors.lastname: Tirado-Gonzalez
authors.forename: Mika
authors.initials: M
authors.lastname: Ito
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoneda
authors.forename: Barna
authors.initials: B
authors.lastname: Vásárhelyi
authors.forename: János
authors.initials: J
authors.lastname: Rigó
authors.forename: Shigeru
authors.initials: S
authors.lastname: Saito
mesh_terms: D000328:Adult; D001323:Autoantibodies; D005260:Female; D037483:Galectin 1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D001323:Autoantibodies; D037483:Galectin 1