title: Preparation and characterization of cross-linked guar gum microspheres: optimization using factorial design.
abstract: In the present work cross-linked guar gum microspheres were prepared for colon specific delivery of ornidazole. Development and optimization of guar gum microspheres for colonic drug delivery was carried out using a 2(4) factorial design based on four independent variables. Microspheres were prepared by emulsification method using glutaraldehyde as cross-linking agent. Morphology and surface characteristics of the formulations were determined by scanning electron microscopy. Particle size of the guar gum microspheres was determined by particle size analyzer. In vitro drug-release studies were performed in conditions simulating stomach-to-colon transit in the presence and absence of rat cecal contents. Only a small fraction of drug was released at acidic pH; however, the release of drug was found to be higher in the presence of rat cecal contents, indicating the susceptibility of guar gum matrix to colonic enzymes released from rat cecal contents. The significance of differences was evaluated by analysis of variance (ANOVA). Differences were considered statistically significant at p<0.05.
authors.affiliation: School of Pharmaceutical Sciences, Rajiv Gandhi Technological University, The State Technical University of Madhya Pradesh, Bhopal, India. rajrgpv@gmail.com
authors.forename: Shukla Raj
authors.initials: SR
authors.lastname: Kumar
authors.forename: Trivedi
authors.initials: T
authors.lastname: Piyush
authors.forename: Ramteke
authors.initials: R
authors.lastname: Suman
authors.forename: Tiwari
authors.initials: T
authors.lastname: Akanksha
mesh_terms: D000818:Animals; D002432:Cecum / Q000187:drug effects / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000138:chemical synthesis / Q000493:pharmacokinetics; D015195:Drug Design; D005685:Galactans / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D008351:Mannans / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D008863:Microspheres; D053149:Plant Gums / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D051381:Rats
chemical_list: D003432:Cross-Linking Reagents; D004337:Drug Carriers; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C007894:guar gum