title: Type-specific human papillomavirus DNA testing with the genotyping array: a comparison of cervical and vaginal sampling.
abstract: OBJECTIVE
  To compare the detection and typing of human papillomavirus (HPV) between vaginal and cervical specimens by using polymerase chain reaction (PCR)-based reverse-blot genotyping arrays.
  
  
  METHODS
  Two hundred and fifty-two women were referred to colposcopy clinics because of suspicious or positive results in a community-based cervical cancer-screening program. Genital tract cells were sampled from the cervix and self-collected from the vagina and tested with the HPV Blot kit.
  
  
  RESULTS
  The HPV Blot kit identified HPV infection in 24.7% of vaginal specimens and in 30.2% of cervical collections. Cervical sampling detected significantly more infections compared to vaginal sampling only for HPV type 52; cervical sampling also detected significantly more high-risk HPV infection overall. The sensitivities of detecting histology ≥ cervical intraepithelial neoplasia (CIN) grade 3 using the HPV Blot in vaginal and cervical specimens were 75.0% (95% CI, 47.6-92.7%) and 87.5% (95% CI, 61.6-98.4%), respectively (P=0.48). Both sampling methods were thus statistically effective at detecting high-grade lesions and cervical cancer (P<0.0001).
  
  
  CONCLUSIONS
  The HPV Blot yielded similar results for both vaginal sampling and cervical sampling in the detection of CIN grade 3 or worse. These findings indicate that self-sampling for HPV testing is a viable cervical cancer screening option.
authors.affiliation: Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taiwan.
authors.forename: Nae-Fang
authors.initials: NF
authors.lastname: Twu
authors.forename: Ming-Shyen
authors.initials: MS
authors.lastname: Yen
authors.forename: Hei-Yu
authors.initials: HY
authors.lastname: Lau
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Chen
authors.forename: Bill Ken-Jen
authors.initials: BK
authors.lastname: Yu
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000302:isolation & purification*; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D011933:Reagent Kits, Diagnostic; D012307:Risk Factors; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D013624:Taiwan / Q000453:epidemiology; D002577:Uterine Cervical Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000821:virology; D014626:Vaginal Smears / Q000379:methods*; D014773:Virology / Q000379:methods; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000821:virology
chemical_list: D011933:Reagent Kits, Diagnostic