title: Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f1).
abstract: Immunoassay methods are generally used for measuring of allergenic substances. However, they need special facilities, skilled handling, and time-consuming procedure. In this work, a fiber-optic immunoassay system which could measure allergen by fluorescent intensities of immune complexes formed by allergens and fluorescently labeled antibodies was established. Immune complexes absorbed on the optical fiber probe surface, and excitation light was injected into the probe, then evanescent field is created in the proximity of the probe. The fluorophores were excited by the evanescent light, and fluorescence was detected by a photo diode. The target allergen detected by our system was Der f1 derived from Dermatophagoides farinae that is one of the house dust mite and major source of inhaled allergens. The fluorophore used labeling on detecting antibody was cyanine 5. The system enabled to detect and quantitatively determine of Der f1. The measurement range was from 0.24 to 250 ng/ml, and the result competes with ELISA. The measurement time was 16 min/sample. The immunoassay system was applied to measurement of Der f1 from actual dust samples. Calculated values of Der f1 showed good correlations between the fiber-optic fluoroimmunoassay and ELISA.
authors.affiliation: Department of Advanced Sciences and Technology for Biomedical Sensors, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Miyajima
authors.forename: Gen
authors.initials: G
authors.lastname: Itabashi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Koshida
authors.forename: Keiko
authors.initials: K
authors.lastname: Tamari
authors.forename: Daishi
authors.initials: D
authors.lastname: Takahashi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kudo
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Saito
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yano
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Shiba
authors.forename: Kohji
authors.initials: K
authors.lastname: Mitsubayashi
mesh_terms: D016902:Air Pollution, Indoor / Q000032:analysis; D000485:Allergens / Q000032:analysis*; D000818:Animals; D040001:Dermatophagoides farinae; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005470:Fluorometry; D007118:Immunoassay; D055100:Optical Fibers
chemical_list: D000485:Allergens