title: Differential RNA-binding activity of the hnRNP G protein correlated with the sex genotype in the amphibian oocyte.
abstract: A proteomic approach has enabled the identification of an orthologue of the splicing factor hnRNP G in the amphibians Xenopus tropicalis, Ambystoma mexicanum, Notophthalmus viridescens and Pleurodeles walt, which shows a specific RNA-binding affinity similar to that of the human hnRN G protein. Three isoforms of this protein with a differential binding affinity for a specific RNA probe were identified in the P. walt oocyte. In situ hybridization to lampbrush chromosomes of P. waltl revealed the presence of a family of hnRNP G genes, which were mapped on the Z and W chromosomes and one autosome. This indicates that the isoforms identified in this study are possibly encoded by a gene family linked to the evolution of sex chromosomes similarly to the hnRNP G/RBMX gene family in mammals.
authors.affiliation: Institut Jacques Monod, UMR 7592, CNRS and Université Paris-Diderot, Museum National d'Histoire Naturelle, U 565, USM 503, UMR 5153, INSERM and CNRS, Paris, France.
authors.forename: Rasha
authors.initials: R
authors.lastname: Kanhoush
authors.forename: Danièle
authors.initials: D
authors.lastname: Praseuth
authors.forename: Caroline
authors.initials: C
authors.lastname: Perrin
authors.forename: Dominique
authors.initials: D
authors.lastname: Chardard
authors.forename: Joëlle
authors.initials: J
authors.lastname: Vinh
authors.forename: May
authors.initials: M
authors.lastname: Penrad-Mobayed
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D005838:Genotype; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000737:chemistry; D006801:Humans; D005810:Multigene Family; D009865:Oocytes / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010999:Pleurodeles / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015347:RNA Probes; D012730:Sex Chromosomes
chemical_list: D029845:Amphibian Proteins; D034541:Heterogeneous-Nuclear Ribonucleoprotein Group F-H; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D010455:Peptides; D020033:Protein Isoforms; C510272:RBMX protein, human; D015347:RNA Probes