title: Cell line studies of glioblastoma and malignant glioma using a newly discovered prenylated chalcone.
abstract: BACKGROUND
  A newly discovered geranyl prenylated chalcone, semisynthesized from naturally occurring nymphaeol C, has the ability to inhibit the growth of CNS1 (glioblastoma) and 13-06 (malignant glioma) cells. A second-order regression model was established to predict the normalized cell viability of CNS1 and 13-06 cells.
  
  
  OBJECTIVE
  The goal of this study is to evaluate the influence of prenylated chalcone on the glioblastoma and malignant glioma cell lines. For the first time, response surface methodology (RSM) has been introduced to perform a cell line study.
  
  
  METHODS
  A newly discovered prenylated chalcone was used. This compound is a member of the flavonoid family and possesses a common phenylbenzopyrone structure. Two independent factors, including prenylated chalcone concentration and uptake time, were carefully evaluated by a 2² factorial design. RSM was introduced as a new method for CNS1 and 13-06 cell line studies.
  
  
  RESULTS
  For CNS1 cells, the least inhibition uptake time was 20.7 h, and the least inhibition dose was 12.4 μg/ml. For 13-06 cells, the best inhibition uptake time was 26.2 h, and the least inhibition dose was 12.0 μg/ml.
  
  
  CONCLUSIONS
  The RSM model successfully predicted the normalized cell viability of CNS1 and 13-06 cells through the use of prenylated chalcone. The results obtained in this study will be useful for further studies on the use of prenylated chalcone.
authors.affiliation: Institute of Basic Medicine Science, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chih-Chuan
authors.initials: CC
authors.lastname: Teng
authors.forename: Chun-I
authors.initials: CI
authors.lastname: Sze
authors.forename: Ting-Chung
authors.initials: TC
authors.lastname: Wang
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Lien
authors.forename: Wayne C
authors.initials: WC
authors.lastname: Liao
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D002599:Chalcone / Q000302:isolation & purification / Q000378:metabolism*; D044950:Flavanones / Q000627:therapeutic use; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D054876:Prenylation / Q000502:physiology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; C562280:nymphaeol C; D002599:Chalcone