title: Bright field neuronal preparation optimized for automatic computerized reconstruction, a case with cerebellar climbing fibers.
authors.affiliation: Department of Systems Neurophysiology, Tokyo Medical and Dental University Graduate School, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan. isugihara.phy1@tmd.ac.jp
authors.forename: Izumi
authors.initials: I
authors.lastname: Sugihara
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D002531:Cerebellum / Q000166:cytology* / Q000502:physiology; D004396:Coloring Agents / Q000493:pharmacokinetics; D003911:Dextrans / Q000493:pharmacokinetics; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods* / Q000639:trends; D008853:Microscopy / Q000295:instrumentation / Q000379:methods / Q000639:trends; D008867:Microtomy / Q000379:methods / Q000639:trends; D056347:Neuroanatomical Tract-Tracing Techniques / Q000379:methods* / Q000639:trends; D009847:Olivary Nucleus / Q000166:cytology / Q000502:physiology; D010363:Pattern Recognition, Automated / Q000379:methods / Q000639:trends; D013194:Staining and Labeling / Q000379:methods* / Q000639:trends
chemical_list: D004396:Coloring Agents; D003911:Dextrans; C076397:biotinylated dextran amine; D001710:Biotin