title: Comparative study of long- and short-pulsed electric fields for treating melanoma in an in vivo mouse model.
abstract: A mouse melanoma model was set up with green fluorescent protein (GFP) expression in vivo. With the same energy, long- (1 ms) and short- (300 ns) pulsed electric fields were delivered to two melanomas injected into the same mouse. The tumor growth and green fluorescence were followed up to compare the different treatment efficacy of long and short pulses. After two days post treatment, short pulse-treated tumors showed a significantly lower tumor volume compared with long pulse-treated tumors (n=8, p<0.05). On 8 experimental animals, a short nanosecond pulsed electric field (nsPEF) had lesser or delayed effects on GFP quenching and greater effects in reducing tumor size. Short pulses produced by nsPEFs can cause melanoma regression with less effect on the plasma membrane.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, PR China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
authors.forename: Xinmei
authors.initials: X
authors.lastname: Chen
authors.forename: Karl H
authors.initials: KH
authors.lastname: Schoenbach
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
authors.forename: R James
authors.initials: RJ
authors.lastname: Swanson
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D004599:Electric Stimulation Therapy / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008546:Melanoma, Experimental / Q000473:pathology / Q000628:therapy*; D051379:Mice; D026741:Physical Therapy Modalities; D012878:Skin Neoplasms / Q000473:pathology / Q000628:therapy*; D016896:Treatment Outcome
chemical_list: D049452:Green Fluorescent Proteins