title: A single amino acid substitution in the 126-kDa protein of pepper mild mottle virus controls replication and systemic movement into upper non-inoculated leaves of bell pepper plants.
abstract: Previously, we generated attenuated variants of pepper mild mottle virus by replacing residue 649 in the 126-kDa replicase protein with various amino acids. Here, we examined the biological properties of the 16 variants that caused either mild mosaic or no mosaic. All but one (A649N) of the mild-mosaic-inducing strains replicated at higher levels in pepper plants and systemically moved at higher rates into the upper non-inoculated leaves than the no-mosaic strains. C1421, previously selected for practical use, not only caused mild symptoms but also had an especially high replication rate in pepper plants and spread more efficiently into the upper non-inoculated leaves.
authors.affiliation: National Agricultural Research Center, Kan-nondai, Tsukuba, Ibaraki, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Nakazono-Nagaoka
authors.forename: T
authors.initials: T
authors.lastname: Omura
authors.forename: T
authors.initials: T
authors.lastname: Uehara-Ichiki
mesh_terms: D019943:Amino Acid Substitution; D002212:Capsicum / Q000821:virology*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000821:virology; D017898:Tobamovirus / Q000472:pathogenicity*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication
chemical_list: D050505:Mutant Proteins; D014764:Viral Proteins