title: Research resource: Comparative nuclear receptor atlas: basal and activated peritoneal B-1 and B-2 cells.
abstract: Naïve murine B cells are typically divided into three subsets based on functional and phenotypic characteristics: innate-like B-1 and marginal zone B cells vs. adaptive B-2 cells, also known as follicular or conventional B cells. B-1 cells, the innate-immune-like component of the B cell lineage are the primary source of natural antibodies and have been shown to modulate autoimmune diseases, human B-cell leukemias, and inflammatory disorders such as atherosclerosis. On the other hand, B-2 cells are the principal mediators of the adaptive humoral immune response and represent an important pharmacological target for various conditions including rheumatoid arthritis, lupus erythematosus, and lymphomas. Using the resources of the Nuclear Receptor Signaling Atlas program, we used quantitative real-time PCR to assess the complement of the 49 murine nuclear receptor superfamily expressed in quiescent and toll-like receptor (TLR)-stimulated peritoneal B-1 and B-2 cells. We report the expression of 24 nuclear receptors in basal B-1 cells and 25 nuclear receptors in basal B-2 cells, with, in some cases, dramatic changes in response to TLR 4 or TLR 2/1 stimulation. Comparative nuclear receptor profiling between B-1 and peritoneal B-2 cells reveals a highly concordant expression pattern, albeit at quantitatively dissimilar levels. We also found that splenic B cells express 23 nuclear receptors. This catalog of nuclear receptor expression in B-1 and B-2 cells provides data to be used to better understand the specific roles of nuclear receptors in B cell function, chronic inflammation, and autoimmune disease.
authors.affiliation: Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
authors.forename: Cody J
authors.initials: CJ
authors.lastname: Diehl
authors.forename: Grant D
authors.initials: GD
authors.lastname: Barish
authors.forename: Michael
authors.initials: M
authors.lastname: Downes
authors.forename: Meng-Yun
authors.initials: MY
authors.lastname: Chou
authors.forename: Sven
authors.initials: S
authors.lastname: Heinz
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Glass
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Evans
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Witztum
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D018956:CD5 Antigens / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D051194:Toll-Like Receptor 1 / Q000235:genetics / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism
chemical_list: D018956:CD5 Antigens; D018160:Receptors, Cytoplasmic and Nuclear; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4