title: Porphyromonas gingivalis stimulates monocyte adhesion to human umbilical vein endothelial cells.
abstract: Monocyte recruitment to the endothelium is a crucial step in the inflammatory response that precedes the development of atherosclerosis. We assessed the effect of Porphyromonas gingivalis on monocyte adhesion to endothelial cells, cytokine production during monocyte/endothelial cell co-culture, and the expression of cell adhesion molecules on human umbilical vein endothelial cells (HUVEC) and their ligands on monocytes. Porphyromonas gingivalis challenge significantly increased the adhesion of THP-1 monocytes to HUVEC, the production of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein 1 (MCP-1) in co-cultures of HUVEC and THP-1 cells, and the transcription and translation of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in HUVEC. The transcription of tumor necrosis factor receptor-associated factor 1 was also increased in HUVEC and THP-1 cells by P. gingivalis infection. Moreover, the stimulation of monocyte adhesion to HUVEC by P. gingivalis infection was partially inhibited by pretreatment with a mixture of anti-ICAM-1, -VCAM, and -E-selectin monoclonal antibodies. These data suggest that adherence between HUVEC and THP-1 cells, followed by the production of cytokines and chemokines, was enhanced by increased expression of cell adhesion molecules on P. gingivalis-sensitized HUVEC, which in turn led to inflammatory atherogenesis.
authors.affiliation: Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan. hashizume.tomomi@nihon-u.ac.jp
authors.forename: Tomomi
authors.initials: T
authors.lastname: Hashizume
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kurita-Ochiai
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D018932:Chemokine CCL2 / Q000096:biosynthesis; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis; D019040:E-Selectin / Q000096:biosynthesis; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology* / Q000502:physiology; D016966:Porphyromonas gingivalis / Q000276:immunology* / Q000502:physiology; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D016207:Cytokines; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1