title: Full genomic analyses of two human G2P[4] rotavirus strains detected in 2005: identification of a caprine-like VP3 gene.
abstract: Although G2P[4] rotaviruses are common causes of infantile diarrhoea, to date only the full genomes of the prototype (strain DS-1) and another old strain, TB-Chen, have been analysed. We report here the full genomic analyses of two Bangladeshi G2P[4] strains, MMC6 and MMC88, detected in 2005. Both the strains exhibited a DS-1-like genotype constellation. Excluding the VP4 and VP7 genes, and except for VP3 of MMC88, the MMC strains were genetically more closely related to the contemporary G2P[4] and several non-G2P[4] human strains than the prototype G2P[4] strain. However, by phylogenetic analyses, the VP2, VP3 (except MMC88), NSP1 and NSP3-5 genes of these strains appeared to share a common origin with those of the prototype strain, whilst their VP1, VP6 and NSP2 genes clustered near a caprine strain. The VP3 gene of MMC88 exhibited maximum relatedness to a local caprine strain, representing the first reported human G2P[4] strain with a gene of animal origin.
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Souvik
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh.
authors.forename: Shyamal Kumar
authors.initials: SK
authors.lastname: Paul
authors.affiliation: Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh.
authors.forename: Mohammad Akram
authors.initials: MA
authors.lastname: Hossain
authors.affiliation: Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
authors.forename: Mohammed Mahbub
authors.initials: MM
authors.lastname: Alam
authors.affiliation: Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
authors.forename: Muzahed Uddin
authors.initials: MU
authors.lastname: Ahmed
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Kobayashi
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D016000:Cluster Analysis; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C442546:VP3 protein, Rotavirus