title: [Study on polymorphism of membrane glycoprotein genes related to human platelet alloantigens].
abstract: OBJECTIVE
  To investigate the polymorphisms of platelet membrane glycoprotein genes related to human platelet alloantigen (HPA)-1 to 17w.
  
  
  METHODS
  The DNA segments of platelet membrane glycoprotein genes related to HPA-1 to 17w were amplified using author's designed primers. The amplification products were purified and directly sequenced to identify the HPA genotype and glycoprotein gene polymorphisms.
  
  
  RESULTS
  Thirteen new single nucleotide polymorphisms (SNPs) and a micro-satellite sequence were found in the glycoprotein genes from the 112 random samples, in which two SNPs (1333G/A and 1960G/A) in ITGB3 gene result in two amino acid change (V419M and E628K) on glycoprotein GPIIIa.
  
  
  CONCLUSIONS
  New variants in platelet membrane glycoprotein genes were identified, which may lead to structure change of platelet membrane glycoprotein and affect the accuracy of partial HPA genotyping method.
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province; Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Lan
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Ying
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xian-guo
authors.initials: XG
authors.lastname: Xu
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Hang-jun
authors.initials: HJ
authors.lastname: Lv
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics*; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D016824:Antigens, Human Platelet; D007519:Isoantigens; D010980:Platelet Membrane Glycoproteins