title: Sequencing transcriptomes in toto.
abstract: The development of next-generation sequencing technologies has enabled the transcriptome to be measured and characterized at a level which was previously unattainable. Shot gun sequencing of RNAs, or RNA-Seq as it is known, is providing the means to simultaneously survey locus activity, transcript-specific expression, sequence content of transcripts and transcriptome discovery. This article discusses the current state of RNA-Seq, its potential for redefining transcriptomics and some of the challenges associated with this revolutionary technology.
authors.affiliation: Queensland Centre for Medical Genomics, Institute for Molecular Bioscience, Brisbane, Australia.
authors.forename: Karin S
authors.initials: KS
authors.lastname: Kassahn
authors.forename: Nic
authors.initials: N
authors.lastname: Waddell
authors.forename: Sean M
authors.initials: SM
authors.lastname: Grimmond
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D020869:Gene Expression Profiling / Q000379:methods*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics*
chemical_list: D012313:RNA