title: Hepatic biotransformation genes and enzymes and PAH metabolites in bile of common sole (Solea solea, Linnaeus, 1758) from an oil-contaminated site in the Mediterranean Sea: a field study.
abstract: The aim of the present field study was to evaluate the response of hepatic biotransformation genes and enzymes of the common sole collected from an area characterized by the presence of an oil-refinery. Based on ∑PAHs levels detected in sediments, three sites were identified: an high-impact site in front of the refinery, a moderate impact site and a reference site at increasing distances from the refinery. Transcription of cyp1a, udpgt and gst genes and related enzyme activities, such as EROD, BROD, MROD, B(a)PMO, UDPGT and GST, were assessed in sole liver. PAHs bile metabolites were measured. The link between phases I and II is discussed with regard to levels of PAHs measured in sediments and fillets. Results provide sequencing data on biotransformation genes essential for further studies on transcriptional responses in common sole and confirm phase I enzyme activities as useful tools for future biomonitoring studies in marine coastal areas.
authors.affiliation: Department of Environmental Sciences "G. Sarfatti", University of Siena, Siena, Italy. trisciani8@unisi.it
authors.forename: Anna
authors.initials: A
authors.lastname: Trisciani
authors.forename: Ilaria
authors.initials: I
authors.lastname: Corsi
authors.forename: Camilla Della
authors.initials: CD
authors.lastname: Torre
authors.forename: Guido
authors.initials: G
authors.lastname: Perra
authors.forename: Silvano
authors.initials: S
authors.lastname: Focardi
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D001711:Biotransformation; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004784:Environmental Monitoring; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D019015:Geologic Sediments / Q000737:chemistry; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008522:Mediterranean Sea; D050216:Metabolic Detoxication, Phase I; D010578:Petroleum / Q000032:analysis / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D029941:Fish Proteins; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1