title: Determination of Ganciclovir and its prodrug Valganciclovir by hydrophilic interaction liquid chromatography-tandem mass spectrometry.
abstract: This manuscript describes the determination of Ganciclovir (GCV), active component of the antiviral drug Valcyte®, and its ester prodrug Valganciclovir (VGC) in human and rat plasma, using liquid chromatography coupled to tandem mass spectrometry. Protein precipitation with acetonitrile was followed by hydrophilic interaction liquid chromatography on a silica column with 4 min run time. After electrospray ionization, the compounds were detected in positive ion selected reaction monitoring (SRM) mode. The lower limits of quantification (LLOQ) were 16 ng/mL for GCV and 4 ng/mL for VGC in human and rat plasma. Inter-day and intra-day precisions and inaccuracies were below 15% and between 85 and 115%, respectively. Five-fold deuterated GCV and VGC were used as internal standards and compensated for any matrix effect. The method was successfully applied to samples from a rat pharmacokinetic study. The feasibility of blood analysis as dried blood spots (DBS) was investigated.
authors.affiliation: F. Hoffmann-La Roche Ltd., Pharma Research, Non-Clinical Safety, Basel, Switzerland. katja.heinig@roche.com
authors.forename: Katja
authors.initials: K
authors.lastname: Heinig
authors.forename: Thomas
authors.initials: T
authors.lastname: Wirz
authors.forename: Almudena
authors.initials: A
authors.lastname: Gajate-Perez
authors.forename: Sara
authors.initials: S
authors.lastname: Belli
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D015774:Ganciclovir / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077562:Valganciclovir
chemical_list: D000077562:Valganciclovir; D015774:Ganciclovir