title: Electrospinning and biocompatibility evaluation of biodegradable polyurethanes based on L-lysine diisocyanate and L-lysine chain extender.
abstract: A series of biodegradable polyurethanes (PUs) were synthesized using poly(ε-caprolactone) diol (PCL) to react with L-lysine ethyl ester diisocyanate (LDI) chain extend with L-lysine ethyl ester (LEE) in solution of DMF. The structure was characterized by gel permeation chromatography, ¹H-NMR, Fourier transform infrared, and DSC analyses. Mechanical property testing showed that their tensile strength rose with increasing the hard segment content with a maximum tensile strength of 34.43 ± 1.73 MPa. The average mass loss for the hydrolytic degradation was only about 13 % in 56 days while this value for the enzymatic degradation was around 95 % in 30 days. The morphological and biomechanical characters of the tubular scaffolds electrospun from the as-prepared PUs were also examined. As the solution concentration was varied from 10 to 18% (w/v), the fiber diameter was progressively increased, and the scaffold tensile strength was enhanced from 2.82 ± 0.16 MPa to 7.07 ± 0.44 MPa, the suture retention strength from 2.48 ± 0.33 to 8.38 ± 0.35 N, and the burst pressure strength from 72 ± 2 to 172 ± 2 kPa, all higher than those of native blood vessels. At the same time, the L-929 mouse fibroblasts (L-929) and human umbilical vein endothelial cells were used in cytotoxicity and cell-adhesion evaluations toward the electrospun scaffolds. The level of toxicity is less than level 1, and cells were found to attach well to and remain viable on the scaffolds.
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, No.5 Zhongguancun South Street, Haidian District, Beijing 100081, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Run-Wu
authors.initials: RW
authors.lastname: Cao
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Ye
authors.forename: Ai-Ying
authors.initials: AY
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Zeng-Guo
authors.initials: ZG
authors.lastname: Feng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D055664:Electrochemical Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D017953:Isocyanates / Q000737:chemistry*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D011140:Polyurethanes / Q000737:chemistry / Q000378:metabolism; D013718:Tensile Strength
chemical_list: D001672:Biocompatible Materials; D017953:Isocyanates; D011140:Polyurethanes; C511489:lysine diisocyanate; D008239:Lysine