title: Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction.
abstract: Regardless of their primary causes, progressive renal fibrosis and tubular atrophy are the main predictors of progression to end-stage renal disease. Basigin/CD147 is a multifunctional molecule-it induces matrix metalloproteinases and hyaluronan, for example-and has been implicated in organ fibrosis. However, the relationship between basigin and organ fibrosis has been poorly studied. We investigated basigin's role in renal fibrosis using a unilateral ureteral obstruction model. Basigin-deficient mice (Bsg(-/-)) demonstrated significantly less fibrosis after surgery than Bsg(+/+) mice. Fewer macrophages had infiltrated in Bsg(-/-) kidneys. Consistent with these in vivo data, primary cultured tubular epithelial cells from Bsg(-/-) mice produced less matrix metalloproteinase and exhibited less motility on stimulation with transforming growth factor β. Furthermore, Bsg(-/-) embryonic fibro blasts produced less hyaluronan and α-smooth muscle actin after transforming growth factor β stimulation. Together, these results demonstrate for the first time that basigin is a key regulator of renal fibrosis. Basigin could be a candidate target molecule for the prevention of organ fibrosis.
authors.affiliation: Department of Biochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Kato
authors.forename: Tomoki
authors.initials: T
authors.lastname: Kosugi
authors.forename: Waichi
authors.initials: W
authors.lastname: Sato
authors.forename: Takuji
authors.initials: T
authors.lastname: Ishimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kojima
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sato
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Shoichi
authors.initials: S
authors.lastname: Maruyama
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yuzawa
authors.forename: Seiichi
authors.initials: S
authors.lastname: Matsuo
authors.forename: Kenji
authors.initials: K
authors.lastname: Kadomatsu
mesh_terms: D000818:Animals; D051926:Basigin / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005355:Fibrosis; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D007674:Kidney Diseases / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D016212:Transforming Growth Factor beta / Q000378:metabolism / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects; D014517:Ureteral Obstruction / Q000150:complications* / Q000378:metabolism / Q000473:pathology
chemical_list: D016212:Transforming Growth Factor beta; D051926:Basigin; D006820:Hyaluronic Acid; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9