title: Comparative production of cellulases by mutants of Penicillium janthinellum NCIM 1171 and its application in hydrolysis of Avicel and cellulose.
abstract: Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparation gave less hydrolysis compared to SmF derived enzymes. This may be due to inactivation of β-glucosidase at 50°C in SSF derived enzyme preparations. SmF derived enzyme preparations contained both thermostable and thermosensitive β-glucosidases where as SSF derived enzyme preparations contained predominantly thermosensitive β-glucosidase. This is the first report on less thermostability of SSF derived β-glucosidase which is the main reason for getting less hydrolysis.
authors.affiliation: NCIM Resource Center, National Chemical Laboratory (CSIR), Pune 411008, Maharashtra, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Singhvi
authors.forename: M G
authors.initials: MG
authors.lastname: Adsul
authors.forename: D V
authors.initials: DV
authors.lastname: Gokhale
mesh_terms: D044602:Cellulases / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism*; D004795:Enzyme Stability; D005285:Fermentation; D009154:Mutation / Q000235:genetics*; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics*; D013379:Substrate Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D002482:Cellulose; D044602:Cellulases; D001617:beta-Glucosidase