title: A LC-MS analysis of acamprosate from human plasma: pharmacokinetic application.
abstract: A rapid and highly sensitive method for the determination of acamprosate (ACM), in human plasma using ESI-LC-MS/MS (electrospray ionization liquid chromatography tandem mass spectrometry) in negative ionization polarity in multiple reactions monitoring (MRM) mode was developed and validated. The procedure involves a simple protein precipitation step. Chromatographic separation was carried out on a Hypersil BDS C(18) column (150 mm × 4.6 mm, 5 µm) with an isocratic mobile phase and a total run time of 2.5 min. The standard calibration curves were linear within the range of 7.04-702.20 ng/mL for ACM (r ≥ 0.990). This study briefly describes the role of ion source design on matrix effects. ACM shows matrix effects in z-spray ionization source design, whereas it has no matrix effects in orthogonal spray ion source design. This method was successfully applied to a pharmacokinetic study after oral administration of acamprosate 333 mg tablet in Indian healthy male volunteers.
authors.affiliation: Bioanalytical Department, Cadila Pharmaceuticals Limited, 1389-Trasad road, Dholka, Gujarat, India. chinmoy_ghosh@yahoo.com
authors.forename: Chinmoy
authors.initials: C
authors.lastname: Ghosh
authors.forename: Vijay
authors.initials: V
authors.lastname: Jha
authors.forename: Chandrakant P
authors.initials: CP
authors.lastname: Shinde
authors.forename: Bhaswat S
authors.initials: BS
authors.lastname: Chakraborty
mesh_terms: D000077443:Acamprosate; D000328:Adult; D000427:Alcohol Deterrents / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000191:economics / Q000379:methods; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000191:economics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods; D013654:Taurine / Q000031:analogs & derivatives* / Q000097:blood; D013997:Time Factors
chemical_list: D000427:Alcohol Deterrents; D013654:Taurine; D000077443:Acamprosate