title: Fate of bisphenol A during treatment with the litter-decomposing fungi Stropharia rugosoannulata and Stropharia coronilla.
abstract: Bisphenol A is an endocrine disrupting compound, which is ubiquitous in the environment due to its wide use in plastic and resin production. Seven day old cultures of the litter-decomposing fungus Stropharia coronilla removed the estrogenic activity of bisphenol A (BPA) rapidly and enduringly. Treatment of BPA with purified neutral manganese peroxidase (MnP) from this fungus also resulted in 100% reduction of estrogenic activity, as analyzed using a bioluminescent yeast assay, and in the formation of polymeric compounds. In cultures of Stropharia rugosoannulata, estrogenic activity also quickly disappeared but temporarily re-emerged in the further course of cultivation. LC-MS analysis of the extracted estrogenic culture liquid revealed [M-H](-) ions with m/z values of 219 and 235. We hypothesize that these compounds are ring fission products of BPA, which still exhibit one intact hydroxyphenyl group to interact with estrogen receptors displayed by the yeast.
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland. grit.kabiersch@helsinki.fi
authors.forename: Grit
authors.initials: G
authors.lastname: Kabiersch
authors.forename: Johanna
authors.initials: J
authors.lastname: Rajasärkkä
authors.forename: René
authors.initials: R
authors.lastname: Ullrich
authors.forename: Marja
authors.initials: M
authors.lastname: Tuomela
authors.forename: Martin
authors.initials: M
authors.lastname: Hofrichter
authors.forename: Marko
authors.initials: M
authors.lastname: Virta
authors.forename: Annele
authors.initials: A
authors.lastname: Hatakka
authors.forename: Kari
authors.initials: K
authors.lastname: Steffen
mesh_terms: D000363:Agaricales / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D001559:Benzhydryl Compounds; D001673:Biodegradation, Environmental; D052244:Endocrine Disruptors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010544:Peroxidases; D010636:Phenols; D012989:Soil Pollutants; C051129:manganese peroxidase; C006780:bisphenol A; D000098826:Bisphenol A Compounds