title: [Clinical significance of measuring hepatitis B virus large surface protein in serum].
abstract: OBJECTIVE
  To explore the significance of serum hepatitis B virus large protein( HBV-LP), HBV-DNA and markers of hepatitis B virus (HBV-M)in the diagnosis of viral replication.
  
  
  METHODS
  Serum HBV-DNA level was quantitatively detected using PCR Real-time polymerase chain reaction, HBV-LP was detected by enzyme-linked immunosorbent assay (ELISA) and HBV markers expression were measured by chemiluminescence immunoassay method in 1886 cases of seurm.
  
  
  RESULTS
  The results of hepatitis virus large protein (HBV-LP) detection and the detection results of HBV-DNA was no significant difference (chi2 = 1.142, P > 0.05). HBV-DNA logarithm of copies and A vaule of HBV-LP was a positive correlation (r = 0.487, P < 0.01). HBV-DNA copies of different groups was significantly different from HBV-LP A values (F = 7.772, P < 0.01). The results of HBV-LP and HBV-DNA detected in different patterns of HBV-M were not significantly different. In 36 healthy people,the detecting results of HBV-DNA and HBV-LP are negative.
  
  
  CONCLUSIONS
  There is a good correlation between the copies of HBV-DNA and the levels of HBV-LP. HBV-LP expression can reflect the replication of HBV.
authors.affiliation: Department of Clinical Laboratory, the Second Affiliated Hospital of Southeast University, Nanjing 210003, China.
authors.forename: Nian-yue
authors.initials: NY
authors.lastname: Wang
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
authors.forename: Yan-wen
authors.initials: YW
authors.lastname: Ge
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
mesh_terms: D016022:Case-Control Studies; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D015870:Gene Expression; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D014759:Viral Envelope Proteins / Q000097:blood* / Q000235:genetics; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; C096362:L protein, hepatitis B virus; D014759:Viral Envelope Proteins