title: Production and activities of chitinases and hydrophobins from Lecanicillium lecanii.
abstract: The production of chitinases and hydrophobins from Lecanicillium lecanii was influenced by the cultivation method and type of carbon source. Crude enzyme obtained from solid-substrate culture presented activities of exochitinases (32 and 51 kDa), endochitinases (26 kDa), β-N-acetylhexosaminidases (61, 80, 96 and 111 kDa). Additionally, submerged cultures produced exochitinases (32 and 45 kDa), endochitinases (10 and 26 kDa) and β-N-acetylhexosaminidases (61, 96 and 111 kDa). β-N-acetylhexosaminidases activity determined in solid-substrate culture with added chitin was ca. threefold (7.58 ± 0.57 U mg(-1)) higher than submerged culture (2.73 + 0.57 U mg(-1)). Similarly, hydrophobins displayed higher activities in solid-substrate culture (627.3 ± 2 μg protein mL(-1)) than the submerged one (57.4 ± 4.7 μg protein mL(-1)). Molecular weight of hydrophobins produced in solid-substrate culture was 7.6 kDa and they displayed surface activity on Teflon.
authors.affiliation: Laboratory of Biopolymers, Biotechnology Department, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco, No. 186, 09340, Mexico City, Mexico.
authors.forename: Zaizy
authors.initials: Z
authors.lastname: Rocha-Pino
authors.forename: Gabriel
authors.initials: G
authors.lastname: Vigueras
authors.forename: Keiko
authors.initials: K
authors.lastname: Shirai
mesh_terms: D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006596:Hexosaminidases / Q000302:isolation & purification / Q000378:metabolism; D006999:Hypocreales / Q000201:enzymology*; D008855:Microscopy, Electron, Scanning; D011138:Polytetrafluoroethylene / Q000737:chemistry; D013172:Spores, Fungal / Q000254:growth & development; D013379:Substrate Specificity; D001619:beta-N-Acetylhexosaminidases / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002686:Chitin; D011138:Polytetrafluoroethylene; C079639:1,4-beta-chitobiosidase; D006596:Hexosaminidases; D002688:Chitinases; D001619:beta-N-Acetylhexosaminidases