title: Genetic polymorphisms of p53 codon 72 and bladder cancer susceptibility: a hospital-based case-control study.
abstract: BACKGROUND
  p53 is one of the most widely investigated molecular markers in bladder cancer and its polymorphisms have been related to individual cancer risks. The objective of this study was to explore the association of p53 codon 72 polymorphism with susceptibility and clinicopathologic characteristics of bladder cancer in a Chinese population.
  
  
  METHODS
  We investigated the impact of p53 codon 72 polymorphism in a hospital-based case-control study of bladder cancer. We tested peripheral blood samples from 120 patients with bladder cancer and 120 healthy individuals of similar age and from the same geographical region. The polymorphisms were analyzed using polymerase chain reaction-restriction fragment length polymorphism assay.
  
  
  RESULTS
  There was an association between smoking status and bladder cancer (odds ratio [OR] = 2.25; 95% confidence interval [95% CI] = 1.31, 3.87; p = 0.003). Patients with bladder cancer had a significantly lower frequency of Arg/Arg (OR = 0.53; 95% CI = 0.31, 0.89; p = 0.02) and Arg allele (OR = 0.66; 95% CI = 0.45, 0.95; p = 0.03) than controls. Patients with invasive bladder cancer had a significantly lower frequency of Arg/Arg (OR = 0.29; 95% CI = 0.10, 0.88; p = 0.03) than those with superficial bladder cancer. When stratifying by the grade and histological type of bladder cancer, we found no statistical association.
  
  
  CONCLUSIONS
  These data suggest that the p53 codon 72 Arg/Arg genotype and Arg allele are associated with a lower risk of bladder cancer in Chinese population.
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Ronggui
authors.initials: R
authors.lastname: Zhang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Chen
authors.forename: Weili
authors.initials: W
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.forename: Chuan
authors.initials: C
authors.lastname: Liu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Lin
authors.forename: Zili
authors.initials: Z
authors.lastname: Hu
authors.forename: Shengjie
authors.initials: S
authors.lastname: Yu
authors.forename: Guangyong
authors.initials: G
authors.lastname: Xu
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006785:Hospitals, University; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D003062:Codon; D016159:Tumor Suppressor Protein p53