title: Topical effects of roflumilast on 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice.
abstract: Phosphodiesterase-4 (PDE-4) inhibitors have a wide range of anti-inflammatory and immunomodulatory activities. Here we examined the effects of roflumilast, a well-known PDE-4 inhibitor, on 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis-like lesions. Roflumilast inhibited DNCB-stimulated IL-1alpha secretion in HaCaT cells, and reduced ear thickness and lymph node weights in BALB/c mice sensitized with DNCB. Topical application of roflumilast to DNCB-induced atopic dermatitis-like skin lesions of NC/Nga mice ameliorated intensity scores and dorsal skin thickness, in parallel with reduced tissue IL-1beta levels and epidermal hyperplasia. On the other hand, no effect on IgE and IL-4 was observed upon roflumilast treatment. Taken together, roflumilast showed beneficial effects against DNCB-induced atopic dermatitis.
authors.affiliation: Center for Metabolic Syndrome Therapeutics, Bio-Organic Science Division, Korea Research Institute of Chemical Technology, Daejeon, Korea.
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Heo
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Cho
authors.forename: Hyae Gyeong
authors.initials: HG
authors.lastname: Cheon
mesh_terms: D000631:Aminopyridines / Q000627:therapeutic use*; D000818:Animals; D001549:Benzamides / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D000242:Cyclic AMP / Q000097:blood; D003521:Cyclopropanes / Q000627:therapeutic use; D003876:Dermatitis, Atopic / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D004137:Dinitrochlorobenzene; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D053582:Interleukin-1alpha / Q000096:biosynthesis; D007509:Irritants; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D058988:Phosphodiesterase 4 Inhibitors / Q000627:therapeutic use*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D018417:Th1 Cells / Q000187:drug effects
chemical_list: D000631:Aminopyridines; D001549:Benzamides; D003521:Cyclopropanes; D004137:Dinitrochlorobenzene; D053582:Interleukin-1alpha; D007509:Irritants; D058988:Phosphodiesterase 4 Inhibitors; C424423:Roflumilast; D007073:Immunoglobulin E; D000242:Cyclic AMP