title: Simian immunodeficiency virus SIVmac239Deltanef vaccination elicits different Tat28-35SL8-specific CD8+ T-cell clonotypes compared to a DNA prime/adenovirus type 5 boost regimen in rhesus macaques.
abstract: Different human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV) vaccine vectors expressing the same viral antigens can elicit disparate T-cell responses. Within this spectrum, replicating variable vaccines, like SIVmac239Δnef, appear to generate particularly efficacious CD8(+) T-cell responses. Here, we sequenced T-cell receptor β-chain (TRB) gene rearrangements from immunodominant Mamu-A 01-restricted Tat(28-35)SL8-specific CD8(+) T-cell populations together with the corresponding viral epitope in four rhesus macaques during acute SIVmac239Δnef infection. Ultradeep pyrosequencing showed that viral variants arose with identical kinetics in SIVmac239Δnef and pathogenic SIVmac239 infection. Furthermore, distinct Tat(28-35)SL8-specific T-cell receptor (TCR) repertoires were elicited by SIVmac239Δnef compared to those observed following a DNA/Ad5 prime-boost regimen, likely reflecting differences in antigen sequence stability.
authors.affiliation: Department of Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Burwitz
authors.forename: Zachary
authors.initials: Z
authors.lastname: Ende
authors.forename: Benjamin
authors.initials: B
authors.lastname: Sudolcan
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Reynolds
authors.forename: Justin M
authors.initials: JM
authors.lastname: Greene
authors.forename: Benjamin N
authors.initials: BN
authors.lastname: Bimber
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Almeida
authors.forename: Monica
authors.initials: M
authors.lastname: Kurniawan
authors.forename: Vanessa
authors.initials: V
authors.lastname: Venturi
authors.forename: Emma
authors.initials: E
authors.lastname: Gostick
authors.forename: Roger W
authors.initials: RW
authors.lastname: Wiseman
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Douek
authors.forename: David A
authors.initials: DA
authors.lastname: Price
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000260:Adenoviruses, Human; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004337:Drug Carriers / Q000008:administration & dosage; D015693:Gene Products, nef / Q000276:immunology*; D005822:Genetic Vectors; D059014:High-Throughput Nucleotide Sequencing; D007117:Immunization, Secondary / Q000379:methods*; D008253:Macaca mulatta; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D018449:SAIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D004337:Drug Carriers; D015693:Gene Products, nef; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018449:SAIDS Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic