title: Simultaneous determination of 2-naphthol and 1-hydroxy pyrene in urine by gas chromatography-mass spectrometry.
abstract: A gas chromatography-mass spectrometric (GC-MS) method was developed for the determination of 2-naphthol (2-NAP) and 1-hydroxypyrene (1-HOP) in human urine. Extraction from urine after the enzyme hydrolysis with β-glucuronidase/arylsulfatase was achieved with a liquid extraction using 5 mL of pentane. After addition of 50 μL of N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MTBDMSTFA) to prevent the loss of 2-NAP during drying, the extract was completely dried and derivatized with MTBDMSTFA for 30 min at 60 °C. The accuracies were in the range of 96-109% at a concentration of 0.5, 10 and 25 μg/L and their precisions were less than 15%. Method detection limits of 2-NAP and 1-HOP were 0.07 and 0.01 μg/L, respectively. This method was used to analyze twenty urine samples, and they were found in the concentration range <0.07-13.7 μg/L (2-NAP) and <0.01-0.88 μg/L (1-HOP). The concentrations of 2-NAP and 1-HOP were well correlated to those of naphthalene and pyrene in blood, respectively.
authors.affiliation: Department of Environmental Education, Kongju National University, Kongju 314-701, Republic of Korea. hshin@kongju.ac.kr
authors.forename: Ho-Sang
authors.initials: HS
authors.lastname: Shin
authors.forename: Hyun-Hee
authors.initials: HH
authors.lastname: Lim
mesh_terms: D000081:Acetamides; D000328:Adult; D000368:Aged; D005260:Female; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009284:Naphthols / Q000097:blood / Q000737:chemistry / Q000652:urine*; D017646:Organosilicon Compounds / Q000737:chemistry; D011721:Pyrenes / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D014269:Trifluoroacetic Acid / Q000737:chemistry; D014556:Urine / Q000737:chemistry
chemical_list: D000081:Acetamides; D005463:Fluoroacetates; D009284:Naphthols; D017646:Organosilicon Compounds; D011721:Pyrenes; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide; D014269:Trifluoroacetic Acid; C033146:1-hydroxypyrene; C028405:2-naphthol