title: Development of a new molecular detection method for Taylorella equigenitalis.
abstract: On PCR amplification of the intervening sequences (IVSs) in the central (helix 45) region within 23S rRNA gene sequences with T. equigenitalis (n = 34), as well as T. asinigenitalis (n = 35) and Bordetella (n = 11) isolates by using the primer pair of f-/r-23STis2, approximately 0.8 kb of the amplicons were generated, sequenced and analyzed. One IVS of approximately 70 bp in length was identified in all the Taylorella organisms but not Bordetella. PCR amplification was further developed for the convenient and rapid molecular detection of T. equigenitalis organisms with the IVS in the helix 45 region within the 23S rRNA genes as target by using the primer pairs (f-IVSde/r-23de). Thus, these results clearly demonstrated that PCR amplification with the primer pair (f-IVSde/r-23de) can be reliable in order to differentiate the T. equigenitalis isolates from both the T. asinigenitalis and Bordetella organisms.
authors.affiliation: Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Fuchinobe, Chuo-ku Sagamihara, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tazumi
authors.forename: Junichi
authors.initials: J
authors.lastname: Hirayama
authors.forename: Kyohei
authors.initials: K
authors.lastname: Hayashi
authors.forename: Sandrine
authors.initials: S
authors.lastname: Petry
authors.forename: John E
authors.initials: JE
authors.lastname: Moore
authors.forename: B Cherie
authors.initials: BC
authors.lastname: Millar
authors.forename: Motoo
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006736:Horses; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020625:Taylorella equigenitalis / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D012338:RNA, Ribosomal, 23S