title: Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS.
abstract: Z-scan fluorescence correlation spectroscopy (FCS) is employed to characterize the interaction between arenicin-1 and supported lipid bilayers (SLBs) of different compositions. Lipid analogue C8-BODIPY 500/510C5-HPC and ATTO 465 labelled arenicin-1 are used to detect changes in lipid and peptide diffusion upon addition of unlabelled arenicin-1 to SLBs. Arenicin-1 decreases lipid mobility in negatively charged SLBs. According to diffusion law analysis, microdomains of significantly lower lipid mobility are formed. The analysis of peptide FCS data confirms the presence of microdomains for anionic SLBs. No indications of microdomain formation are detected in SLBs composed purely of zwitterionic lipids. Additionally, our FCS results imply that arenicin-1 exists in the form of oligomers and/or aggregates when interacting with membranes of both compositions.
authors.affiliation: J. Heyrovský Institute of Physical Chemistry v.v.i, Academy of Sciences of the Czech Republic, Dolejškova 2155/3, 182 23 Prague 8, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Macháň
authors.forename: Martin
authors.initials: M
authors.lastname: Hof
authors.forename: Tatsiana
authors.initials: T
authors.lastname: Chernovets
authors.forename: Maxim N
authors.initials: MN
authors.lastname: Zhmak
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Ovchinnikova
authors.forename: Jan
authors.initials: J
authors.lastname: Sýkora
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism*; D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008954:Models, Biological; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D008055:Lipids