title: [Effect of Itk down regulation on cytokines production in Jurkat cell].
abstract: OBJECTIVE
  To study the effect of Itk down regulation on Jurkat cell proliferation and inflammatory cytokines production, and provide useful data for Itk as an attractive target for potential drugs.
  
  
  METHODS
  Three shRNAs against different region of Itk were constructed and cotransfected with pEGFP-C1-hItk. The shRNA, which can knock down Itk, was selected and packed into lentivirus. After Jurkat cells were transfected with shRNA lentivirus, the change of Itk protein expression, cell proliferation and cytokines production was observed.
  
  
  RESULTS
  Itk mRNA was reduced about 55% in Jurkat cells transfected with Itk-shRNA1, compared with that in control cells shRNAnon (P < 0.05). Knocking down Itk expression had a profound inhibitory effect on Jurkat cell proliferation. In addition, there was a substantial decrease in level of cytokines, such as IL-2, IL-5, IL-10 and IFN-gamma, produced by cell transfected with Itk-shRNA1.
  
  
  CONCLUSIONS
  Knocking down Itk expression can inhibit Jurkat cell proliferation and inflammatory cytokines production.
authors.affiliation: Hepatobiliary and Enteric Surgery Research Center of Ministry of Health, Center South University, Changsha, Hunan 410008, China.
authors.forename: Hai-lan
authors.initials: HL
authors.lastname: Yao
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.forename: Zong-hui
authors.initials: ZH
authors.lastname: Xiao
authors.forename: Ji-sheng
authors.initials: JS
authors.lastname: Han
authors.forename: Yang-de
authors.initials: YD
authors.lastname: Zhang
authors.forename: Bo-yun
authors.initials: BY
authors.lastname: Huang
authors.forename: Zhe-wei
authors.initials: ZW
authors.lastname: Liu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology; D019169:Jurkat Cells / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000276:immunology*
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015848:Interleukin-5; D016753:Interleukin-10; D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase