title: Evaluation of reproductive/developmental and repeated dose (subchronic) toxicity and cytogenetic effects in rats of a roofing asphalt fume condensate by nose-only inhalation.
abstract: A Type III Built-up Roofing Asphalt (BURA) fume condensate was evaluated for subchronic systemic toxicity and reproductive/developmental toxicity screening in Wistar rats, by OECD protocol 422 and OECD cytogenetic protocol 474. Animals were exposed by nose-only inhalation to target concentrations of 30, 100 and 300 mg/m³ total hydrocarbons (actual concentrations, 30.0, 100.1 and 297.3 mg/m³). The study was performed to assess potential hazards from asphalt fumes to which humans could be exposed during application. No adverse effects were seen for spermology, reproductive or developmental parameters or early postnatal development of offspring from day 1 to 4 postpartum. BURA fume condensate did not induce any significant increases in micronucleus frequency in polychromatic erythrocytes of rat bone marrow nor was neurobehavioral toxicity observed at any dose. Systemic effects were slight and seen at doses above those measured at work sites. The systemic NOAEC of 100 mg/m³ for males was based on decreased body weight gain, food consumption and increased absolute and relative lung wet weight correlated with slight histological changes in the lung, primarily adaptive in nature at 300 mg/m³. The female NOAEC of 30 mg/m³ was based on a statistically significant increase in relative wet lung weight at higher doses, correlated with slight histopathologic effects in the lungs at the highest dose. However, no increase in relative lung weight was seen in breeding females at 100 mg/m³.
authors.affiliation: Marathon Petroleum (Retired), 539 S. Main Street, Room 1092, Findlay, OH 45840, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Parker
authors.forename: C A
authors.initials: CA
authors.lastname: Schreiner
authors.forename: N
authors.initials: N
authors.lastname: Hallmark
authors.forename: A J
authors.initials: AJ
authors.lastname: Kriech
authors.forename: L V
authors.initials: LV
authors.lastname: Osborn
authors.forename: R
authors.initials: R
authors.lastname: Fuhst
authors.forename: J
authors.initials: J
authors.lastname: Buschmann
authors.forename: H
authors.initials: H
authors.lastname: Ernst
authors.forename: T
authors.initials: T
authors.lastname: Hansen
authors.forename: G
authors.initials: G
authors.lastname: Pohlmann
authors.forename: A
authors.initials: A
authors.lastname: Preiss
authors.forename: C
authors.initials: C
authors.lastname: Ziemann
mesh_terms: D000280:Administration, Inhalation; D000281:Administration, Intranasal; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D020732:Cytogenetic Analysis / Q000379:methods; D004334:Drug Administration Schedule; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D006838:Hydrocarbons / Q000008:administration & dosage* / Q000633:toxicity*; D019570:Inhalation Exposure / Q000009:adverse effects; D008168:Lung / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology
chemical_list: D006838:Hydrocarbons; C006647:asphalt