title: Development and application of a method to investigate drug-metabolizing enzyme inhibitors using sparteine for probe of cytochrome P450 2D6 and tris(2,2'-bipyridine)ruthenium(II)-electrogenerated chemiluminescence detection.
abstract: We studied the detection of drug-metabolizing enzyme inhibitiors using column-switching high performance liquid chromatography with tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)(3)(2+))-electrogenerated chemiluminescence detection. This can be applied to evaluate the genetic diversity concerning the ability of cytochrome P450 (CYP) 2D6 to metabolize drug in vitro. We demonstrated the ability of CYP2D6 to enable us to examine drugs metabolizing enzyme inhibition with high performance and sensitivity. This method can be applied to investigate metabolite inhibitors of CYP2D6 in vitro and in vivo. Thus, Metixene was found to be a potential CYP2D6 inhibitor.
authors.affiliation: College of Pharmacy, Nihon University, Funabashi, Chiba, Japan.
authors.forename: Yuu
authors.initials: Y
authors.lastname: Shibano
authors.forename: Shiwori
authors.initials: S
authors.lastname: Taki
authors.forename: Aoi
authors.initials: A
authors.lastname: Miyamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Uchikura
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D056831:Coordination Complexes; D065690:Cytochrome P-450 CYP2D6 Inhibitors; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D011817:Rabbits; D013034:Sparteine / Q000737:chemistry* / Q000494:pharmacology; D017975:Ruthenium Compounds
chemical_list: D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D065690:Cytochrome P-450 CYP2D6 Inhibitors; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D013034:Sparteine; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds