title: Preparation and properties of nano-hydroxyapatite/PCL-PEG-PCL composite membranes for tissue engineering applications.
abstract: Nano-hydroxyapatite (n-HA)/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) composite membranes were prepared by solvent casting and evaporation method. The structure and properties of the membranes were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), water contact angle measurements, in vitro hydrolytic degradation, mechanical test, and cell culture. The effect of n-HA content on physical-chemical properties of the n-HA/PCEC composite membranes was studied. The results showed that the shape and size of micropores of the composite membranes changed with n-HA content increased; the tensile strength decreased with the increase of n-HA content. The osteoblast cell was cultured on the membranes, good cell attachment and growth manner were observed after postseeding for 1 day. MTT assays showed that the n-HA/PCEC membranes had no negative effect on the cell viability and proliferation. These results suggested that the obtained n-HA/PCEC composite membranes in this study might have prospective applications in tissue engineering field.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Shao Zhi
authors.initials: SZ
authors.lastname: Fu
authors.forename: Xiu Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Fan
authors.forename: Hang
authors.initials: H
authors.lastname: Liang
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Qian
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D008567:Membranes, Artificial; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D011092:Polyethylene Glycols; D017886:Durapatite