title: Application of an acid proteinase from Monascus purpureus to reduce antigenicity of bovine milk whey protein.
abstract: An acid proteinase from Monascus purpureus No. 3403, MpuAP, was previously purified and some characterized in our laboratory (Agric Biol Chem 48:1637-1639, 1984). However, further information about this enzyme is lacking. In this study, we investigated MpuAP's comprehensive substrate specificity, storage stability, and prospects for reducing antigenicity of whey proteins for application in the food industry. MpuAP hydrolyzed primarily five peptide bonds, Gln(4)-His(5), His(10)-Leu(11), Ala(14)-Leu(15), Gly(23)-Phe(24) and Phe(24)-Phe(25) in the oxidized insulin B-chain. The lyophilized form of the enzyme was well preserved at 30-40°C for 7 days without stabilizers. To investigate the possibility of reducing the antigenicity of the milk whey protein, enzymatic hydrolysates of the whey protein were evaluated by inhibition ELISA. Out of the three main components of whey protein, casein and α-lactalbumin were efficiently degraded by MpuAP. The sequential reaction of MpuAP and trypsin against the whey protein successfully degraded casein, α-lactalbumin and β-lactoglobulin with the highest degree of hydrolysis. As a result, the hydrolysates obtained by using the MpuAP-trypsin combination showed the lowest antigenicity compared with the single application of pepsin, trypsin or pepsin-trypsin combination. Therefore, the overall result suggested that the storage-stable MpuAP and trypsin combination will be a productive approach for making hypoallergic bovine milk whey protein hydrolysates.
authors.affiliation: United Graduate School of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan. monascus@yahoo.com
authors.forename: P L Nilantha
authors.initials: PL
authors.lastname: Lakshman
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Tachibana
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Toki
authors.initials: T
authors.lastname: Taira
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Suganuma
authors.forename: Worapot
authors.initials: W
authors.lastname: Suntornsuk
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D006868:Hydrolysis; D007768:Lactalbumin / Q000378:metabolism; D007782:Lactoglobulins / Q000378:metabolism; D008894:Milk Proteins / Q000276:immunology* / Q000378:metabolism*; D032741:Monascus / Q000201:enzymology*; D010206:Papain / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000276:immunology; D014357:Trypsin / Q000378:metabolism; D000067816:Whey Proteins
chemical_list: D000941:Antigens; D002364:Caseins; D007782:Lactoglobulins; D008894:Milk Proteins; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D007768:Lactalbumin; D010447:Peptide Hydrolases; D014357:Trypsin; D010206:Papain; D010434:Pepsin A