title: Identification, characterization and expression profiles of Chironomus riparius glutathione S-transferase (GST) genes in response to cadmium and silver nanoparticles exposure.
abstract: In this study, we report the identification and characterization of 13 cytosolic GST genes in Chironomus riparius from Expressed Sequence Tags (ESTs) database generated using pyrosequencing. Comparative and phylogenetic analyses were undertaken with Drosophila melanogaster and Anopheles gambiae GSTs and 3 Delta, 4 Sigma, 1 each in Omega, Epsilon, Theta, Zeta and 2 unclassified classes of GSTs were identified and characterized. The relative mRNA expression levels of all of the C. riparius GSTs (CrGSTs) genes under different developmental stages were varied with low expression in the larval stage. The antioxidant role of CrGSTs was studied by exposing fourth instar larvae to a known oxidative stress inducer Paraquat and the relative mRNA expression to different concentrations of cadmium (Cd) and silver nanoparticles (AgNPs) for various time intervals were also studied. All the CrGSTs showed up- or down regulation to varying levels based upon the concentration, and duration of exposure. The highest mRNA expression was noticed in Delta3, Sigma4 and Epsilon1 GST class in all treatments. These results show the role of CrGST genes in defense against oxidative stress and its potential as a biomarker to Cd and AgNPs exposure.
authors.affiliation: School of Environmental Engineering and Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul, Republic of Korea.
authors.forename: Prakash M Gopalakrishnan
authors.initials: PM
authors.lastname: Nair
authors.forename: Jinhee
authors.initials: J
authors.lastname: Choi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000852:Anopheles / Q000201:enzymology; D002104:Cadmium / Q000633:toxicity*; D002683:Chironomidae / Q000187:drug effects / Q000201:enzymology*; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism*; D007814:Larva / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000633:toxicity; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012834:Silver / Q000633:toxicity*
chemical_list: D018076:DNA, Complementary; D002104:Cadmium; D012834:Silver; D005982:Glutathione Transferase; D010269:Paraquat