title: High-throughput single-molecule optofluidic analysis.
abstract: We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.
authors.forename: Soohong
authors.initials: S
authors.lastname: Kim
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Streets
authors.forename: Ron R
authors.initials: RR
authors.lastname: Lin
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
authors.forename: Shimon
authors.initials: S
authors.lastname: Weiss
authors.forename: Devdoot S
authors.initials: DS
authors.lastname: Majumdar
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D057166:High-Throughput Screening Assays; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D012321:DNA-Directed RNA Polymerases