title: Aldose reductase (AKR1B3) regulates the accumulation of advanced glycosylation end products (AGEs) and the expression of AGE receptor (RAGE).
abstract: Diabetes results in enhanced chemical modification of proteins by advanced lipoxidation end products (ALEs) and advanced glycation end products (AGEs) precursors. These modifications have been linked to the development of several secondary diabetic complications. Our previous studies showed that aldose reductase (AR; AKR1B3) catalyzes the reduction of ALEs and AGEs precursors; however, the in vivo significance of this metabolic pathway during diabetes and obesity has not been fully assessed. Therefore we examined the role of AR in regulating ALEs and AGEs formation in murine models of diet-induced obesity and streptozotocin-induced diabetes. In comparison with wild-type (WT) and AR-null mice fed normal chow, mice fed a high-fat (HF) diet (42% kcal fat) showed increased accumulation of AGEs and protein-acrolein adducts in the plasma. AGEs and acrolein adducts were also increased in the epididymal fat of WT and AR-null mice fed a HF diet. Deletion of AR increased the accumulation of 4-hydroxy-trans-2-nonenal (HNE) protein adduct in the plasma and increased the expression of the AGE receptor (RAGE) in HF fed mice. No change in AGEs formation was observed in the kidneys of HF-fed mice. In comparison, renal tissue from AR-null mice treated with streptozotocin showed greater AGE accumulation than streptozotocin-treated WT mice. These data indicated that AR regulated the accumulation of lipid peroxidation derived aldehydes and AGEs under conditions of severe, but not mild, hyperglycemia and that deletion of AR increased RAGE-induction via mechanisms that were independent of AGEs accumulation.
authors.affiliation: Diabetes and Obesity Center, University of Louisville, Louisville, KY 40202, USA. spbaba01@louisville.edu
authors.forename: Shahid P
authors.initials: SP
authors.lastname: Baba
authors.forename: Jason
authors.initials: J
authors.lastname: Hellmann
authors.forename: Sanjay
authors.initials: S
authors.lastname: Srivastava
authors.forename: Aruni
authors.initials: A
authors.lastname: Bhatnagar
mesh_terms: D000171:Acrolein / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism; D000449:Aldehyde Reductase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000201:enzymology / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects; D004822:Epididymis / Q000473:pathology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D017127:Glycation End Products, Advanced / Q000097:blood / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D009765:Obesity / Q000097:blood / Q000201:enzymology / Q000209:etiology / Q000378:metabolism; D010084:Oxidation-Reduction; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D004041:Dietary Fats; D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D000171:Acrolein; D000449:Aldehyde Reductase; C027576:4-hydroxy-2-nonenal