title: [Pilot study on MRI of human colon adenocarcinoma cells labeled with superparamagnetic iron oxide in vitro].
abstract: OBJECTIVE
  To study the feasibility of MRI of human colon adenocarcinoma cell line (Lovo) labeled with superparamagnetic iron oxide(SPIO) nanoparticles in vitro.
  
  
  METHODS
  Lovo cells (5 × 10(5) and 1 × 10(6)) were cultured in medium containing different SPIO nanoparticles (50 microl and 500 microl). Transmission electron microscopy was used to observe cellular ultrastructure and to determine the uptake and distribution of particles in Lovo cells at 1-, 3-, 6-hours. MRI of Lovo cells was performed with T1WI, T2WI sequences. Unlabeled cells were used as controls.
  
  
  RESULTS
  Uptake of SPIO nanoparticles occurred within 6 hours. On T1 weighted imaging, there was no significant difference in signal intensity between the experimental groups and the control group. On T2 weighted imaging, there was no significant difference in signal intensity between the experimental groups and the control group after culture of 1 h. Signal intensity began to decrease in 1 × 10(6) Lovo cells labeled with 500 microl SPIO nanoparticle after 3 hours culture. Signal intensity decreased in all the experimental groups after 6 hours culture.
  
  
  CONCLUSIONS
  Human colon adenocarcinoma cell line (Lovo) can be labeled with SPIO nanoparticles, and the labeled cells can be imaged with MRI equipment.
authors.affiliation: Department of Radiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Shi-Ting
authors.initials: ST
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Can-Hui
authors.initials: CH
authors.lastname: Sun
authors.forename: Peng-Xin
authors.initials: PX
authors.lastname: Qiu
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Xin-Tao
authors.initials: XT
authors.lastname: Shuai
authors.forename: Zi-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Quan-Fei
authors.initials: QF
authors.lastname: Meng
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology; D006801:Humans; D007501:Iron; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D053758:Nanoparticles; D010087:Oxides; D010865:Pilot Projects; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D010087:Oxides; D007501:Iron