title: Nanochitosan crosslinked with polyacrylamide as the chiral stationary phase for open-tubular capillary electrochromatography.
abstract: Nanoparticles exhibiting favorable surface-to-volume ratios create efficient stationary phases for electrochromatography. New nanomaterials derived from chitosan (CS) were immobilized onto modified capillaries for use as the chiral stationary phase (CSP) in open-tubular electrochromatography. This immobilization was achieved through the copolymerization of glycidyl methacrylate-modified nano-CS with methacrylamide (MAA) and bis-acrylamide crosslinkers (forming the MAA-CS capillary) rather than the attachment of nano-CS to the copolymer of glycidyl methacrylate, MAA, and bis-acrylamide (forming the MAA+CS capillary). The completed MAA-CS capillary and its precursors were examined by SEM and ATR-IR measurements. Before separating chiral samples, the MAA-CS capillary was characterized by electroosmotic flow measurements at varying pH values, concentrations, and volume percentages of organic modifiers in the running buffers. Tryptophan enantiomers were well separated by the MAA-CS capillary, whereas no enantioselectivity was observed in the MAA+CS capillary. With the addition of 80% MeOH into the phosphate buffer, the chiral separation of (±)-catechin was accomplished in a normal-phase mode. However, the new CSP has its limitations, as only two groups of α-tocopherol stereoisomers were separated.
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan. cjl@mail.cmu.edu.tw
authors.forename: Jian-Lian
authors.initials: JL
authors.lastname: Chen
authors.forename: Kai-Hsin
authors.initials: KH
authors.lastname: Hsieh
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000379:methods*; D002392:Catechin / Q000032:analysis; D048271:Chitosan / Q000737:chemistry*; D053841:Electroosmosis / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D014364:Tryptophan / Q000032:analysis; D024502:alpha-Tocopherol / Q000032:analysis; D000099166:Polyacrylamides
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D002392:Catechin; D048271:Chitosan; D014364:Tryptophan; D024502:alpha-Tocopherol; D000099166:Polyacrylamides; C045985:methacrylamide