title: Microbiological purification of L-arabitol from xylitol mother liquor.
abstract: As a rare sugar alcohol, L-arabitol can be used in food and can prevent extra fat deposits in the intestinal tract. Commercially, L-arabitol is prepared from pure L-arabinose by hydrogenation, which needs a high temperature and high pressure, leading to a high production cost for Larabitol. Therefore, this study describes a novel L-arabitol production method based on biological purification from the xylitol mother liquor, a cheap and readily available raw material that contains a high concentration of Larabitol. First, a novel Bacillus megaterium strain was screened that can utilize xylitol, sorbitol, and mannitol, yet not L-arabitol. The isolated strain was inoculated into a medium containing the xylitol mother liquor under formulated culture conditions, where a high L-arabitol yield (95%) and high purity (80%) were obtained when the medium was supplemented with 50 g/l of xylitol mother liquor. Upon further purification of the fermentation broth by ion exchange and decolorization, L-arabitol was crystallized with a purity of 98.5%.
authors.affiliation: College of Chemical Engineering and Ecology, Guangxi University for Nationality, Nanning, 530007, China.
authors.forename: Mingguo
authors.initials: M
authors.lastname: Jiang
authors.forename: Ben
authors.initials: B
authors.lastname: Wang
authors.forename: Lifang
authors.initials: L
authors.lastname: Yang
authors.forename: Shuangjun
authors.initials: S
authors.lastname: Lin
authors.forename: Hairong
authors.initials: H
authors.lastname: Cheng
mesh_terms: D001410:Bacillus megaterium / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D012988:Soil Microbiology; D013402:Sugar Alcohols / Q000302:isolation & purification* / Q000378:metabolism*; D014993:Xylitol / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013402:Sugar Alcohols; D014993:Xylitol; C014999:arabitol