title: Cell differentiation and matrix organization in engineered teeth.
abstract: Embryonic dental cells were used to check a series of criteria to be achieved for tooth engineering. Implantation of cultured cell-cell re-associations led to crown morphogenesis, epithelial histogenesis, organ vascularization, and root and periodontium development. The present work aimed to investigate the organization of predentin/dentin, enamel, and cementum which formed and mineralized after implantation. These implants were processed for histology, transmission electron microscopy, x-ray microanalysis, and electron diffraction. After two weeks of implantation, the re-associations showed gradients of differentiating odontoblasts. There were ciliated, polarized, and extended cell processes in predentin/dentin. Ameloblasts became functional. Enamel crystals showed a typical oriented arrangement in the inner and outer enamel. In the developing root, odontoblasts differentiated, cementogenesis occurred, and periodontal ligament fibroblasts interacted with the root surface and newly formed bone. The implantation of cultured dental cell re-associations allows for reproduction of complete functional differentiation at the cell, matrix, and mineral levels.
authors.affiliation: INSERM UMR 977, Faculté de Médecine, 11, rue Humann, F-67085 Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Nait Lechguer
authors.forename: M L
authors.initials: ML
authors.lastname: Couble
authors.forename: N
authors.initials: N
authors.lastname: Labert
authors.forename: S
authors.initials: S
authors.lastname: Kuchler-Bopp
authors.forename: L
authors.initials: L
authors.lastname: Keller
authors.forename: H
authors.initials: H
authors.lastname: Magloire
authors.forename: F
authors.initials: F
authors.lastname: Bleicher
authors.forename: H
authors.initials: H
authors.lastname: Lesot
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000648:ultrastructure; D000566:Amelogenesis; D000818:Animals; D002454:Cell Differentiation; D016764:Cell Polarity; D002478:Cells, Cultured; D025021:Cementogenesis; D003460:Crystallization; D003810:Dentinogenesis; D004577:Electron Probe Microanalysis; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000637:transplantation; D004658:Enamel Organ / Q000166:cytology; D005109:Extracellular Matrix / Q000648:ultrastructure*; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D009024:Morphogenesis; D018919:Neovascularization, Physiologic; D009804:Odontoblasts / Q000166:cytology / Q000648:ultrastructure; D010513:Periodontal Ligament / Q000254:growth & development; D033581:Stem Cell Transplantation; D023822:Tissue Engineering; D019228:Tooth Crown / Q000254:growth & development*; D014092:Tooth Root / Q000254:growth & development*