title: MYB46 modulates disease susceptibility to Botrytis cinerea in Arabidopsis.
abstract: In this study, we show that the Arabidopsis (Arabidopsis thaliana) transcription factor MYB46, previously described to regulate secondary cell wall biosynthesis in the vascular tissue of the stem, is pivotal for mediating disease susceptibility to the fungal pathogen Botrytis cinerea. We identified MYB46 by its ability to bind to a new cis-element located in the 5' promoter region of the pathogen-induced Ep5C gene, which encodes a type III cell wall-bound peroxidase. We present genetic and molecular evidence indicating that MYB46 modulates the magnitude of Ep5C gene induction following pathogenic insults. Moreover, we demonstrate that different myb46 knockdown mutant plants exhibit increased disease resistance to B. cinerea, a phenotype that is accompanied by selective transcriptional reprogramming of a set of genes encoding cell wall proteins and enzymes, of which extracellular type III peroxidases are conspicuous. In essence, our results substantiate that defense-related signaling pathways and cell wall integrity are interconnected and that MYB46 likely functions as a disease susceptibility modulator to B. cinerea through the integration of cell wall remodeling and downstream activation of secondary lines of defense.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, 46022 Valencia, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Ramírez
authors.forename: Astrid
authors.initials: A
authors.lastname: Agorio
authors.forename: Alberto
authors.initials: A
authors.lastname: Coego
authors.forename: Javier
authors.initials: J
authors.lastname: García-Andrade
authors.forename: M José
authors.initials: MJ
authors.lastname: Hernández
authors.forename: Begoña
authors.initials: B
authors.lastname: Balaguer
authors.forename: Pieter B F
authors.initials: PB
authors.lastname: Ouwerkerk
authors.forename: Ignacio
authors.initials: I
authors.lastname: Zarra
authors.forename: Pablo
authors.initials: P
authors.lastname: Vera
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D020171:Botrytis / Q000472:pathogenicity*; D002473:Cell Wall / Q000737:chemistry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D008031:Lignin / Q000378:metabolism; D016254:Mutagenesis, Insertional; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D011401:Promoter Regions, Genetic; D018749:RNA, Plant; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C525564:Myb46 protein, Arabidopsis; D018749:RNA, Plant; D014157:Transcription Factors; D008031:Lignin