title: Immunogenicity and protective efficacy in dogs of an MF59™-adjuvanted vaccine against recombinant canine/porcine coronavirus.
abstract: Recently, canine coronavirus (CCoV) strains with putative recombinant origin with porcine transmissible gastroenteritis virus (TGEV) were shown to be widespread in Europe. In this study, a killed vaccine against TGEV-like CCoV strains, included in the new subtype CCoV-IIb, was developed through inactivation with betapropiolactone and emulsification with MF59™ adjuvant. Safety, immunogenicity and efficacy of the developed vaccine were evaluated in vivo. Five 10-week-old beagle pups were administered (three weeks apart) two vaccine doses, whereas two animals served as unvaccinated controls. The vaccine was shown to be safe as no local neither systemic reactions were observed after first and second dose administration. Serum antibodies against CCoV were detected in vaccinates starting from study day 14 (by enzyme-linked immunosorbent assay) or 28 (by virus neutralisation test). Subsequent challenge with virulent CCoV-IIb resulted in the development of mild gastroenteric disease in control pups, whereas vaccinates did not display clinical signs. Faecal shedding of the challenge virus occurred in both treatment groups, but vaccinated dogs were found to shed very low viral titres in comparison to controls. The developed vaccine may help control the CCoV-IIb-induced disease (and active virus circulation) in environments, such as kennels and shelters, where the pathogenic potential of this virus is greater as a consequence of predisposing factors and concurrent infections.
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Rossana
authors.initials: R
authors.lastname: Sciarretta
authors.forename: Valeriana
authors.initials: V
authors.lastname: Colao
authors.forename: Michele
authors.initials: M
authors.lastname: Losurdo
authors.forename: Cristiana
authors.initials: C
authors.lastname: Catella
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Del Giudice
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017939:Coronavirus, Canine / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D009500:Neutralization Tests / Q000662:veterinary; D011136:Polysorbates; D013185:Squalene / Q000276:immunology; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D000276:Adjuvants, Immunologic; C089950:MF59 oil emulsion; D011136:Polysorbates; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D013185:Squalene