title: The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus.
abstract: The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed.
authors.affiliation: Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de Rio Preto, São José do Rio Preto 15090-000, Brazil.
authors.forename: Roberta V M
authors.initials: RV
authors.lastname: Bronzoni
authors.forename: Maria C F S
authors.initials: MC
authors.lastname: Madrid
authors.forename: Danilo V B
authors.initials: DV
authors.lastname: Duarte
authors.forename: Vanessa O A
authors.initials: VO
authors.lastname: Pellegrini
authors.forename: Carolina C
authors.initials: CC
authors.lastname: Pacca
authors.forename: Ana C V
authors.initials: AC
authors.lastname: Carmo
authors.forename: Cleslei F
authors.initials: CF
authors.lastname: Zanelli
authors.forename: Sandro R
authors.initials: SR
authors.lastname: Valentini
authors.forename: Carolina
authors.initials: C
authors.lastname: Santacruz-Pérez
authors.forename: João A R G
authors.initials: JA
authors.lastname: Barbosa
authors.forename: Carol S
authors.initials: CS
authors.lastname: Lutz
authors.forename: Paula
authors.initials: P
authors.lastname: Rahal
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Nogueira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012367:RNA, Viral; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000378:metabolism*; D015005:Yellow fever virus / Q000235:genetics / Q000378:metabolism
chemical_list: C096041:NS5 protein, flavivirus; D012367:RNA, Viral; D017412:Ribonucleoprotein, U1 Small Nuclear; D017361:Viral Nonstructural Proteins