title: CD4+ T cell effects on CD8+ T cell location defined using bioluminescence.
abstract: T lymphocytes of the CD8+ class are critical in delivering cytotoxic function and in controlling viral and intracellular infections. These cells are "helped" by T lymphocytes of the CD4+ class, which facilitate their activation, clonal expansion, full differentiation and the persistence of memory. In this study we investigated the impact of CD4+ T cells on the location of CD8+ T cells, using antibody-mediated CD4+ T cell depletion and imaging the antigen-driven redistribution of bioluminescent CD8+ T cells in living mice. We documented that CD4+ T cells influence the biodistribution of CD8+ T cells, favoring their localization to abdominal lymph nodes. Flow cytometric analysis revealed that this was associated with an increase in the expression of specific integrins. The presence of CD4+ T cells at the time of initial CD8+ T cell activation also influences their biodistribution in the memory phase. Based on these results, we propose the model that one of the functions of CD4+ T cell "help" is to program the homing potential of CD8+ T cells.
authors.affiliation: David H Smith Center for Microbiology and Immunology, Aab Institute for Biomedical Research, University of Rochester Medical Center, Rochester, New York, United States of America.
authors.forename: Mitra
authors.initials: M
authors.lastname: Azadniv
authors.forename: William J
authors.initials: WJ
authors.lastname: Bowers
authors.forename: David J
authors.initials: DJ
authors.lastname: Topham
authors.forename: Ian N
authors.initials: IN
authors.lastname: Crispe
mesh_terms: D000818:Animals; D000941:Antigens / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D007156:Immunologic Memory; D016023:Integrins / Q000032:analysis; D008163:Luminescent Measurements; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D014018:Tissue Distribution
chemical_list: D000941:Antigens; D016023:Integrins