title: Time-course changes in potential biomarkers detected using a metabonomic approach in Walker 256 tumor-bearing rats.
abstract: A metabonomic approach based on complementary hydrophilic interaction chromatography and reversed-phase liquid chromatography combined with tandem mass spectrometry and time-course analysis of metabolites was implemented to find more reliable potential biomarkers in urine of Walker 256 tumor-bearing rats. A major challenge in metabonomics is distinguishing reliable biomarkers that are closely associated with the genesis and progression of diseases from those that are unrelated but altered significantly. In this study, these biomarkers were selected according to the change trends of discriminating metabolites during the genesis and progression of cancer. Seven consecutive batches of urine samples from preinoculation to 16 days after were collected and analyzed. Multivariate analysis revealed 87 discriminating metabolites. Time-course analysis of discriminating metabolites was used to select more reliable biomarkers with regular and reasonable change trends. Finally, 47 were found and 15 were identified including 12 carnitine derivatives, 2 amino acid derivatives, 1 nucleoside. On the basis of time-course behaviors of these potential biomarkers, we hypothesize such disruption might result from elevated cell proliferation, reduced β-oxidation of fatty acids, and poor renal tubular reabsorption. These studies demonstrate that this method can help to find more reliable potential biomarkers and provide valuable biochemical insights into metabolic alterations in tumor-bearing biosystems.
authors.affiliation: Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Shen
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Chen
authors.forename: Jianghao
authors.initials: J
authors.lastname: Sun
authors.forename: Ruiping
authors.initials: R
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiuming
authors.initials: J
authors.lastname: He
authors.forename: Yaping
authors.initials: Y
authors.lastname: Tian
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Song
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.forename: Zeper
authors.initials: Z
authors.lastname: Abliz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry* / Q000652:urine; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D008297:Male; D055432:Metabolomics / Q000379:methods*; D015394:Molecular Structure; D009369:Neoplasms / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors
chemical_list: D015415:Biomarkers