title: IL-10-producing regulatory B10 cells inhibit intestinal injury in a mouse model.
abstract: B cells mediate multiple functions that influence immune and inflammatory responses. In mice, the addition of dextran sulfate sodium (DSS) to drinking water leads to immediate intestinal injury. Dextran sulfate sodium-induced intestinal injury serves as an experimental animal model for human ulcerative colitis. The contribution of B cells to DSS-induced intestinal injury is unclear. In this study, we show that DSS-induced intestinal injury was more severe in CD19-deficient (CD19(-/-)) mice than in wild-type mice. These inflammatory responses were negatively regulated by a unique IL-10-producing CD1d(hi)CD5(+) regulatory B cell subset (B10 cells) that was absent in CD19(-/-) mice and represented only 1% to 2% of splenic B220(+) cells in wild-type mice. Remarkably, adoptive transfer of these B10 cells from wild-type mice reduced inflammation in CD19(-/-) mice in an IL-10-dependent manner. These results demonstrate that IL-10 production from regulatory B10 cells regulates DSS-induced intestinal injury. These findings may provide new insights and therapeutic approaches for treating ulcerative colitis.
authors.affiliation: Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Yanaba
authors.forename: Ayumi
authors.initials: A
authors.lastname: Yoshizaki
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Asano
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kadono
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Tedder
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sato
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism; D055610:Antigens, CD1d / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018956:CD5 Antigens / Q000378:metabolism; D003092:Colitis / Q000276:immunology / Q000473:pathology; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D007422:Intestines / Q000276:immunology* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology
chemical_list: D018941:Antigens, CD19; D055610:Antigens, CD1d; D018956:CD5 Antigens; D016753:Interleukin-10; D016264:Dextran Sulfate