title: Genetic polymorphism of Plasmodium vivax msp1p, a paralog of merozoite surface protein 1, from worldwide isolates.
abstract: Plasmodium vivax msp1p, a paralog of the candidate vaccine antigen P. vivax merozoite surface protein 1, possesses a signal peptide at its N-terminus and two epidermal growth factor-like domains at its C-terminus with a glycosylphosphatidylinositol attachment site. The msp1p gene locus may have originated by a duplication of the msp1 gene locus in a common ancestor of the analyzed Plasmodium species and lost from P. yoelii, P. berghei, and P. falciparum during their evolutionary history. Full-length sequences of the msp1p gene were generally highly conserved; they had a few amino acid substitutions, one highly polymorphic E/Q-rich region, and a single-to-triple hepta-peptide repeat motif. Twenty-one distinguishable allelic types (A1-A21) of the E/Q-rich region were identified from worldwide isolates. Among them, four types were detected in isolates from South Korea. The length polymorphism of the E/Q-rich region might be useful as a genetic marker for population structure studies in malaria-endemic areas.
authors.affiliation: Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Gangwon-do, Republic of Korea. ethan@kangwon.ac.kr
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaneko
authors.forename: Jetsumon
authors.initials: J
authors.lastname: Sattabongkot
authors.forename: Jun-Hu
authors.initials: JH
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeo
authors.forename: Takafumi
authors.initials: T
authors.lastname: Tsuboi
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Ayala
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Eun-Taek
authors.initials: ET
authors.lastname: Han
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D017780:Malaria Vaccines / Q000235:genetics; D016780:Malaria, Vivax / Q000469:parasitology; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010966:Plasmodium vivax / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D056910:Republic of Korea; D016415:Sequence Alignment
chemical_list: D005819:Genetic Markers; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1