title: A resveratrol and polyphenol preparation suppresses oxidative and inflammatory stress response to a high-fat, high-carbohydrate meal.
abstract: BACKGROUND
  High-fat, high-carbohydrate (HFHC) meals are known to induce oxidative and inflammatory stress, an increase in plasma endotoxin concentrations, and an increase in the expression of suppressor of cytokine signaling-3 (SOCS-3).
  
  
  OBJECTIVE
  The intake of a nutritional supplement containing resveratrol and muscadine grape polyphenols reduces HFHC meal-induced oxidative and inflammatory stress and stimulates the activity of the antioxidant transcription factor, NF-E2-related factor-2 (Nrf-2), and its downstream targets.
  
  
  METHODS
  Ten normal, healthy subjects were given a 930-kcal HFHC meal either with placebo or with the supplement. Indices of oxidative stress, inflammation, Nrf-2 binding activity, the concentrations of endotoxin (lipopolysaccharide) and lipoprotein binding protein (LBP), and the expression of toll-like receptor 4 (TLR-4), CD14, IL-1β, TNFα, SOCS-3, Keap-1, NAD(P)H:quinone oxidoreductase-1 (NQO-1), and GST-P1 were measured.
  
  
  RESULTS
  The intake of the supplement suppressed the meal-induced elevations of plasma endotoxin and LBP concentrations, the expression of p47(phox), TLR-4, CD14, SOCS-3, IL-1β, and Keap-1, while enhancing Nrf-2 binding activity and the expression of NQO-1 and GST-P1 genes.
  
  
  CONCLUSIONS
  A supplement containing resveratrol and muscadine polyphenols suppresses the increase in oxidative stress, lipopolysaccharide and LBP concentrations, and expression of TLR-4, CD14, IL-1β and SOCS-3 in mononuclear cells after an HFHC meal. It also stimulates specific Nrf-2 activity and induces the expression of the related antioxidant genes, NQO-1 and GST-P1. These results demonstrate the acute antioxidant and antiinflammatory effects of resveratrol and polyphenolic compounds in humans in the postprandial state.
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, State University of New York at Buffalo, and Kaleida Health, Buffalo, New York 14209, USA.
authors.forename: Husam
authors.initials: H
authors.lastname: Ghanim
authors.forename: Chang Ling
authors.initials: CL
authors.lastname: Sia
authors.forename: Kelly
authors.initials: K
authors.lastname: Korzeniewski
authors.forename: Teekam
authors.initials: T
authors.lastname: Lohano
authors.forename: Sanaa
authors.initials: S
authors.lastname: Abuaysheh
authors.forename: Anuritha
authors.initials: A
authors.lastname: Marumganti
authors.forename: Ajay
authors.initials: A
authors.lastname: Chaudhuri
authors.forename: Paresh
authors.initials: P
authors.lastname: Dandona
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D016207:Cytokines / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D004041:Dietary Fats / Q000009:adverse effects*; D045468:Fallopia japonica / Q000737:chemistry; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis; D027843:Vitis / Q000737:chemistry
chemical_list: D000975:Antioxidants; D016207:Cytokines; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005419:Flavonoids; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; C106644:SOCS3 protein, human; D013267:Stilbenes; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D051197:Toll-Like Receptor 4; D004247:DNA; D000077185:Resveratrol