title: [Spatiotemporal expression patterns of three vernalization genes in wheat].
abstract: To identify spatiotemporal expression patterns of vernalization genes in common wheat, we analyzed expression characteristics of several vernalization genes (VRN1, VRN2 and VRN3) in the wheat cultivars 'Chinese spring' and 'Luohan 2' by RT-PCR. The VRN1 gene was expressed at different levels in the leaves and roots at the 3-leaf stage, stems, flag leaves at the grain-filling stage, anthers, ovules, and developing seeds in 'Chinese spring'. Expression of VRN1 increased before flowering date, then decreased after flowering time. Expression of VRN1 was not detected in dry seeds or seeds germination. Expression patterns of VRN1 in 'Luohan 2' were similar to those in 'Chinese spring', except that it was not expressed in roots or in the leaves at the 3-leaf stage in 'Luohan 2'. Expression of VRN2 was only detected in the leaves at the 3-leaf stage and in the embryo buds during seeds germination. The Spatiotemporal expression of VRN3 was similar to that of VRN1, except that VRN3 was not expressed in roots. These results improved our understanding of the molecular regulation of vernalization genes in common wheat.
authors.affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Yuan
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Li
authors.forename: Fanrong
authors.initials: F
authors.lastname: Meng
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D035264:Flowers / Q000235:genetics* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D014908:Triticum / Q000235:genetics*