title: Oncogramme, a new promising method for individualized breast tumour response testing for cancer treatment.
abstract: BACKGROUND
  Breast cancer is the most widely spread cancer in the world, attracting much research and individualized tumour response testing (ITRT) methods are now used to individualize patient chemotherapeutic administrations. A new ITRT method was developed with optimized processing.
  
  
  METHODS
  Breast tumour fragments were separated and the cells seeded in a foetal calf serum-free defined medium. After various chemotherapeutic treatments, cytotoxicity was determined by cell death detection with calcein acetoxymethyl and ethidium homodimer labelling.
  
  
  RESULTS
  The culture medium allowed breast tumour cell proliferation in culture, while preventing fibroblastic cell survival. Moreover, the cell death analysis gave rise to a chemoresistance profile called an Oncogramme, with statistically significant values.
  
  
  CONCLUSIONS
  The Oncogramme is a new ITRT method which can predict patient cell sensitivities to chemotherapeutics and should be validated by a new phase I clinical trial.
authors.affiliation: Oncomedics, ESTER Technopole, Limoges, France. s.giraud@oncomedics.com
authors.forename: S
authors.initials: S
authors.lastname: Giraud
authors.forename: E
authors.initials: E
authors.lastname: Loum
authors.forename: B
authors.initials: B
authors.lastname: Bessette
authors.forename: V
authors.initials: V
authors.lastname: Fermeaux
authors.forename: C
authors.initials: C
authors.lastname: Lautrette
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D003520:Cyclophosphamide / Q000008:administration & dosage; D000077143:Docetaxel; D019008:Drug Resistance, Neoplasm; D015251:Epirubicin / Q000008:administration & dosage; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000378:metabolism; D057285:Precision Medicine; D011379:Prognosis; D043823:Taxoids / Q000008:administration & dosage; D014407:Tumor Cells, Cultured
chemical_list: D043823:Taxoids; D000077143:Docetaxel; D015251:Epirubicin; D007633:Keratins; D003520:Cyclophosphamide; D005472:Fluorouracil