title: A new trick (hydroxyl radical generation) for an old vitamin (B12).
abstract: Photolysis of hydroxocobalamin in the presence of plasmid DNA (pBR322) results in DNA cleavage. Temporal control of hydroxyl radical production and DNA strand scission by hydroxocobalamin was demonstrated using a 2-deoxyribose assay and a plasmid relaxation assay, respectively. The light-driven hydroxocobalamin-mediated catalytic formation of hydroxyl radicals was demonstrated using radical scavenging studies of DNA cleavage and via recycling of a hydroxocobalamin-resin conjugate several times without loss of efficacy.
authors.affiliation: Department of Chemistry, Division of Medicinal Chemistry and Natural Products, University of North Carolina, Chapel Hill, North Carolina 27599, United States. tshell@email.unc.edu
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Shell
authors.forename: David S
authors.initials: DS
authors.lastname: Lawrence
mesh_terms: D002384:Catalysis; D017665:Hydroxyl Radical / Q000737:chemistry; D008027:Light; D015394:Molecular Structure; D014805:Vitamin B 12 / Q000737:chemistry*
chemical_list: D017665:Hydroxyl Radical; D014805:Vitamin B 12