title: [Expression of survivin gene and its significance in the carcinogenesis and development of non-small cell lung cancer].
abstract: BACKGROUND
  To study the expression of survivin mRNA in non-small cell lung cancer (NSCLC), and to explore its relationship with carcinogenesis, development invasion and metastasis of NSCLC.
  
  
  METHODS
  In situ hybridization was applied to detect survivin mRNA expression in 12 normal bronchial epithelium, 9 dysplasia, 34 NSCLC and 12 metastatic lymph nodes. The relationship between survivin expression and clinicopathological characteristics was analyzed.
  
  
  RESULTS
  In normal bronchial epithelium, dysplasia, NSCLC and metastatic lymph nodes, the positive rate of survivin mRNA expression were 16.67% (2/12), 33.33% (3/9), 61.76% (21/34), and 91.67% (11/12), respectively. There were significant differences in survivin mRNA expression between lung cancer and normal bronchial epithelium ( P < 0.01), as well as between metastatic lymph nodes and normal bronchial epithelium ( P < 0.001). There were remarkably higher survivin mRNA expressions in poor- and moderate-differentiated groups than that in well-differentiated group ( P =0.003, P =0.004). The expression of survivin mRNA was not related to histologic classification and lymph node status ( P > 0.05, P > 0.05).
  
  
  CONCLUSIONS
  Survivin mRNA expression may play an important role in the carcinogenesis and development of NSCLC. It may be a new target in gene therapy of lung cancer through blocking or down-regulating survivin mRNA expression to recover the normal regulation mechanism of apoptosis.
authors.affiliation: Department of Geriatric, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R.China.
authors.forename: Lin
authors.initials: L
authors.lastname: Tao
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Mingqiu
authors.initials: M
authors.lastname: Liu