title: Local vascularized flaps for augmentation of Reinke's space.
abstract: OBJECTIVE
  The purpose of this study is to describe and test a novel surgical strategy for augmentation of Reinke's space using vascularized flaps: a thyroid ala perichondrium flap (TAP) and a composite thyroid ala perichondrium flap (CTAP) from the anterior larynx. We hypothesized that these specially designed vascularized flaps would remain viable once inset into the lamina propria, and that they would not disrupt rheologic, biomechanical, and histologic properties of the native vocal fold.
  
  
  METHODS
  Experimental. In vivo canine model.
  
  
  METHODS
  The length and volume of test flaps harvested in six adult human cadaveric larynges were analyzed to determine suitability for use in augmentation in the lamina propria. Also, 12 beagles randomly underwent unilateral placement of either TAP or CTAP, which were designed in accordance with the human adult cadaveric experiments. Flap perfusion was measured before and after harvest with laser Doppler. After 1 month, the beagles were humanely sacrificed and their larynges subjected to aerodynamic and acoustic evaluation using an excised larynx apparatus. The vocal fold lamina propria of four larynges--two TAP and two CTAP--underwent rheologic evaluation using a simple-shear rheometer. The remaining eight larynges underwent quantitative histologic and immunohistochemical evaluation. The survival and complication (swallowing, airway, local wound) rates of all dogs were noted.
  
  
  RESULTS
  Initial studies with adult human cadaveric larynges established that TAP and CTAP possessed length and volume greater than native lamina propria. In the canine experiments, the perfusion change in the flaps was similar between flap groups. The damping ratio (ζ), dynamic viscosity (η'), elastic shear modulus (G'), and viscous shear modulus (G″) of treated and untreated native vocal folds were not statistically different. The glottic function measures of vocal efficiency, laryngeal resistance, jitter, shimmer, and harmonics-to-noise ratio (HNR) of treated and normal larynges were not statistically different. Similarly, the values for collagen, elastin, and glycosaminoglycans (GAGs) in treated and untreated vocal folds were not statistically different. Also, neither neochrondrogenesis nor neoosteogenesis was detected in any treated vocal fold. The values for vascular and cellular proliferation in treated and untreated vocal folds were not statistically different. All test dogs survived and had no complications related to swallowing, airway distress, or the local wound.
  
  
  CONCLUSIONS
  The test flaps described and tested in this study appear to have conceptually attractive features for augmentation of Reinke's space. When placed in an in vivo setting TAP and CTAP did not reveal unfavorable vascular, rheologic, aerodynamic, acoustic, or histologic characteristics. There was no unanticipated morbidity or mortality to the test animals. Long-term viability of these flaps is unknown. TAP and CTAP may open novel pathways for correction of glottic defects and may offer crossover opportunities with tissue engineering techniques.
authors.affiliation: Department of Surgery/Division of Otolaryngology-Head and Neck Surgery , University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA. dailey@surgery.wisc.edu
authors.forename: Seth H
authors.initials: SH
authors.lastname: Dailey
authors.forename: McLean
authors.initials: M
authors.lastname: Gunderson
authors.forename: Roger
authors.initials: R
authors.lastname: Chan
authors.forename: Jose
authors.initials: J
authors.lastname: Torrealba
authors.forename: Miwako
authors.initials: M
authors.lastname: Kimura
authors.forename: Nathan V
authors.initials: NV
authors.lastname: Welham
mesh_terms: D000328:Adult; D000818:Animals; D002102:Cadaver; D004285:Dogs; D006801:Humans; D009092:Mucous Membrane / Q000601:surgery; D013524:Surgical Flaps / Q000098:blood supply*; D013957:Thyroid Cartilage; D014827:Vocal Cords / Q000601:surgery*