title: 14-3-3 η inhibits chondrogenic differentiation of ATDC5 cell.
abstract: It was previously shown that 14-3-3 η is overexpressed in the synovial fluid of patients with joint inflammation, which is often associated with growth failure. In this study, we investigated the role of 14-3-3 η in chondrogenesis using ATDC5 cells. Upon treatment with TNF-α, cells overexpressed 14-3-3 η with inhibition of chondrogenesis. Chondrogenesis was also inhibited by overexpression of 14-3-3 η without TNF-α treatment, whereas silencing of 14-3-3 η promoted chondrogenic differentiation. Further, G1 phase arrest was inhibited by overexpression of 14-3-3 η. In summary, we suggest that 14-3-3 η plays a regulatory role in chondrogenic differentiation.
authors.affiliation: Laboratory of Cellular and Molecular Biochemistry, School of Life Sciences and Biotechnology, Korea University, 1, 5-Ka, Anam-Dong, Sungbuk-Ku, Seoul 136-701, Republic of Korea.
authors.forename: Hee Eun
authors.initials: HE
authors.lastname: Yoon
authors.forename: Ki Soon
authors.initials: KS
authors.lastname: Kim
authors.forename: Ick Young
authors.initials: IY
authors.lastname: Kim
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D020219:Chondrogenesis / Q000187:drug effects / Q000235:genetics; D016193:G1 Phase / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology / Q000502:physiology
chemical_list: D048948:14-3-3 Proteins; D007328:Insulin; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha