title: Singlet Oxygen Sensor Green®: photochemical behavior in solution and in a mammalian cell.
abstract: The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green(®) (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated.
authors.affiliation: Center for Oxygen Microscopy and Imaging (COMI), Department of Chemistry, Aarhus University, Århus C, Denmark.
authors.forename: Anita
authors.initials: A
authors.lastname: Gollmer
authors.forename: Jacob
authors.initials: J
authors.lastname: Arnbjerg
authors.forename: Frances H
authors.initials: FH
authors.lastname: Blaikie
authors.forename: Brian Wett
authors.initials: BW
authors.lastname: Pedersen
authors.forename: Thomas
authors.initials: T
authors.lastname: Breitenbach
authors.forename: Kim
authors.initials: K
authors.lastname: Daasbjerg
authors.forename: Marianne
authors.initials: M
authors.lastname: Glasius
authors.forename: Peter R
authors.initials: PR
authors.lastname: Ogilby
mesh_terms: D016477:Artifacts; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008856:Microscopy, Fluorescence; D055668:Photochemical Processes / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D059010:Single-Cell Analysis / Q000379:methods; D026082:Singlet Oxygen / Q000032:analysis / Q000378:metabolism; D012996:Solutions / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis
chemical_list: D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D012996:Solutions; D026082:Singlet Oxygen