title: Survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in manure and manure-amended soil under tropical climatic conditions in Sub-Saharan Africa.
abstract: OBJECTIVE
  To establish the fate of Escherichia coli O157:H7 and Salmonella Typhimurium in manure and manure-amended agricultural soils under tropical conditions in Sub-Saharan Africa.
  
  
  RESULTS
  Survival of nonvirulent E. coli O157:H7 and Salm. Typhimurium at 4 and 7 log CFU g(-1) in manure and manure-amended soil maintained at ≥80% r.h. or exposed to exclusive field or screen house conditions was determined in the Central Agro-Ecological Zone of Uganda. Maintaining the matrices at high moisture level promoted the persistence of high-density inocula and enhanced the decline of low-density inocula in the screen house, but moisture condition did not affect survival in the field. The large majority of the survival kinetics displayed complex patterns corresponding to the Double Weibull model. The two enteric bacteria survived longer in manure-amended soil than in manure. The 7 log CFU g(-1) E. coli O157:H7 and Salm. Typhimurium survived for 49-84 and 63-98 days, while at 4 log CFU g(-1) , persistence was 21-28 and 35-42 days, respectively.
  
  
  CONCLUSIONS
  Under tropical conditions, E. coli O157:H7 and Salm. Typhimurium persisted for 4 and 6 weeks at low inoculum density and for 12 and 14 weeks at high inoculum density, respectively.
  
  
  CONCLUSIONS
  Persistence in the tropics was (i) mostly shorter than previously observed in temperate regions thus suggesting that biophysical conditions in the tropics might be more detrimental to enteric bacteria than in temperate environments; (ii) inconsistent with published data isothermally determined previously hence indicating the irrelevance of single point isothermal data to estimate survival under dynamic temperature conditions.
authors.affiliation: Department of Food Science and Post Harvest Technology, Faculty of Agriculture and Environment, Gulu University, Gulu, Uganda Division of Soil and Water Management, Department of Earth and Environmental Sciences, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium Department of Food Science and Technology, Makerere University, Kampala, Uganda Division of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Ongeng
authors.forename: C
authors.initials: C
authors.lastname: Muyanja
authors.forename: A H
authors.initials: AH
authors.lastname: Geeraerd
authors.forename: D
authors.initials: D
authors.lastname: Springael
authors.forename: J
authors.initials: J
authors.lastname: Ryckeboer
mesh_terms: D017954:Africa South of the Sahara; D000383:Agriculture; D019453:Escherichia coli O157 / Q000254:growth & development*; D008372:Manure / Q000382:microbiology; D050296:Microbial Viability; D012486:Salmonella typhimurium / Q000254:growth & development*; D012988:Soil Microbiology; D014329:Tropical Climate
chemical_list: D008372:Manure