title: Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR.
abstract: The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field.
authors.affiliation: Centro de Citricultura Sylvio Moreira/IAC, Rod. Anhanguera Km 158, Cordeirópolis, SP, Brazil.
authors.forename: K S
authors.initials: KS
authors.lastname: Kubo
authors.forename: V M
authors.initials: VM
authors.lastname: Novelli
authors.forename: M
authors.initials: M
authors.lastname: Bastianel
authors.forename: E C
authors.initials: EC
authors.lastname: Locali-Fabris
authors.forename: R
authors.initials: R
authors.lastname: Antonioli-Luizon
authors.forename: M A
authors.initials: MA
authors.lastname: Machado
authors.forename: J
authors.initials: J
authors.lastname: Freitas-Astúa
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000821:virology*; D017931:DNA Primers; D008925:Mites / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012209:Rhabdoviridae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers