title: Differential proteome analysis of mature and germinated embryos of Araucaria angustifolia.
abstract: Araucaria angustifolia is an endangered Brazilian native conifer tree. The aim of the present work was to identify differentially expressed proteins between mature and germinated embryos of A. angustifolia, using one and two dimensional gel electrophoresis approaches followed by protein identification by tandem mass spectrometry. The identities of 32 differentially expressed protein spots from two dimensional gel maps were successfully determined, including proteins and enzymes involved in storage mobilization such as the vicilin-like storage protein and proteases. A label free approach, based on spectral counts, resulted in detection of 10 and 14 mature and germinated enriched proteins, respectively. Identified proteins were mainly related to energetic metabolism pathways, translational processes, oxidative stress regulation and cellular signaling. The integrated use of both strategies permitted a comprehensive protein expression overview of changes in germinated embryos in relation to matures, providing insights into the this process in a recalcitrant seed species. Applications of the data generated on the monitoring and control of in vitro somatic embryos were discussed.
authors.affiliation: Department of Botany, Institute of Biosciences, University of Sao Paulo, 05422-970 Sao Paulo, SP, Brazil. balbuenat@missouri.edu
authors.forename: Tiago S
authors.initials: TS
authors.lastname: Balbuena
authors.forename: Leonardo
authors.initials: L
authors.lastname: Jo
authors.forename: Fernanda P
authors.initials: FP
authors.lastname: Pieruzzi
authors.forename: Leonardo L C
authors.initials: LL
authors.lastname: Dias
authors.forename: Vanildo
authors.initials: V
authors.lastname: Silveira
authors.forename: Claudete
authors.initials: C
authors.lastname: Santa-Catarina
authors.forename: Magno
authors.initials: M
authors.lastname: Junqueira
authors.forename: Jay J
authors.initials: JJ
authors.lastname: Thelen
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Eny I S
authors.initials: EI
authors.lastname: Floh
mesh_terms: D001938:Brazil; D030541:Databases, Genetic; D015180:Electrophoresis, Gel, Two-Dimensional; D018525:Germination / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D064028:Tracheophyta / Q000196:embryology* / Q000235:genetics / Q000254:growth & development
chemical_list: D010940:Plant Proteins