title: The effect of 13C enrichment in the glassing matrix on dynamic nuclear polarization of [1-13C]pyruvate.
abstract: Dimethyl sulfoxide (DMSO) can effectively form a glassy matrix necessary for dynamic nuclear polarization (DNP) experiments. We tested the effects of (13)C enrichment in DMSO on DNP of [1-(13)C]pyruvate doped with trityl radical OX063Me. We found that the polarization build-up time τ of pyruvate in (13)C-labeled DMSO glassing solution is twice as fast as the unenriched DMSO while the nuclear magnetic resonance enhancement was unchanged. This indicates that (13)C-(13)C spin diffusion is a limiting factor in the kinetics of DNP in this system, but it has a minimal effect on the absolute value of polarization achievable for the target.
authors.affiliation: Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
authors.forename: Lloyd
authors.initials: L
authors.lastname: Lumata
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kovacs
authors.forename: Craig
authors.initials: C
authors.lastname: Malloy
authors.forename: A Dean
authors.initials: AD
authors.lastname: Sherry
authors.forename: Matthew
authors.initials: M
authors.lastname: Merritt
mesh_terms: D002247:Carbon Isotopes; D004058:Diffusion; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019289:Pyruvic Acid / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D014867:Water; D019289:Pyruvic Acid; D004121:Dimethyl Sulfoxide