title: Crystallization and preliminary X-ray crystallographic studies of β-transaminase from Mesorhizobium sp. strain LUK.
abstract: β-Transaminase (β-TA) catalyzes the transamination reaction between β-aminocarboxylic acids and keto acids. This enzyme is a particularly suitable candidate for use as a biocatalyst for the asymmetric synthesis of enantiochemically pure β-amino acids for pharmaceutical purposes. The β-TA from Mesorhizobium sp. strain LUK (β-TAMs) belongs to a novel class in that it shows β-transaminase activity with a broad and unique substrate specificity. In this study, β-TAMs was overexpressed in Escherichia coli with an engineered C-terminal His tag. β-TAMs was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 2.5 Å from a crystal that belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 90.91, b = 192.17, c = 52.75 Å.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.forename: Ok Kyeung
authors.initials: OK
authors.lastname: Park
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Bae
authors.forename: Tae-ho
authors.initials: TH
authors.lastname: Jang
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Yoon
authors.forename: Kyoung Hun
authors.initials: KH
authors.lastname: Do
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Park
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006639:Histidine / Q000737:chemistry; D019751:Intramolecular Transferases / Q000737:chemistry; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015202:Protein Engineering; D017386:Sequence Homology, Amino Acid; D013237:Stereoisomerism; D013379:Substrate Specificity; D000637:Transaminases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014169:Transformation, Bacterial; D014961:X-Ray Diffraction
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D007544:Isopropyl Thiogalactoside; D006639:Histidine; D000637:Transaminases; D019751:Intramolecular Transferases