title: A truncated retrotransposon disrupts the GRM1 coding sequence in Coton de Tulear dogs with Bandera's neonatal ataxia.
abstract: BACKGROUND
  Bandera's neonatal ataxia (BNAt) is an autosomal recessive cerebellar ataxia that affects members of the Coton de Tulear dog breed.
  
  
  OBJECTIVE
  To identify the mutation that causes BNAt.
  
  
  METHODS
  The study involved DNA from 112 Cotons de Tulear (including 15 puppies with signs of BNAt) and 87 DNA samples from dogs of 12 other breeds.
  
  
  METHODS
  The BNAt locus was mapped with a genome-wide association study (GWAS). The coding exons of positional candidate gene GRM1, which encodes metabotropic glutamate receptor 1, were polymerase chain reaction (PCR)-amplified and resequenced. A 3-primer PCR assay was used to genotype individual dogs for a truncated retrotransposon inserted into exon 8 of GRM1.
  
  
  RESULTS
  The GWAS indicated that the BNAt locus was in a canine chromosome 1 region that contained candidate gene GRM1. Resequencing this gene from BNAt-affected puppies indicated that exon 8 was interrupted by the insertion of a 5'-truncated retrotransposon. All 15 BNAt-affected puppies were homozygous for the insert, whereas all other Cotons de Tulear were heterozygotes (n = 43) or homozygous (n = 54) for the ancestral allele. None of the 87 dogs from 12 other breeds had the insertion allele.
  
  
  CONCLUSIONS
  BNAt is caused by a retrotransposon inserted into exon 8 of GRM1. A DNA test for the GRM1 retrotransposon insert can be used for genetic counseling and to confirm the diagnosis of BNAt.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA.
authors.forename: R
authors.initials: R
authors.lastname: Zeng
authors.forename: F H G
authors.initials: FH
authors.lastname: Farias
authors.forename: G S
authors.initials: GS
authors.lastname: Johnson
authors.forename: S D
authors.initials: SD
authors.lastname: McKay
authors.forename: R D
authors.initials: RD
authors.lastname: Schnabel
authors.forename: J E
authors.initials: JE
authors.lastname: Decker
authors.forename: J F
authors.initials: JF
authors.lastname: Taylor
authors.forename: C S
authors.initials: CS
authors.lastname: Mann
authors.forename: M L
authors.initials: ML
authors.lastname: Katz
authors.forename: G C
authors.initials: GC
authors.lastname: Johnson
authors.forename: J R
authors.initials: JR
authors.lastname: Coates
authors.forename: D P
authors.initials: DP
authors.lastname: O'Brien
mesh_terms: D017668:Age of Onset; D000818:Animals; D002524:Cerebellar Ataxia / Q000235:genetics / Q000662:veterinary*; D004252:DNA Mutational Analysis / Q000662:veterinary; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005091:Exons; D005260:Female; D055106:Genome-Wide Association Study; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D016254:Mutagenesis, Insertional; D009154:Mutation; D016366:Open Reading Frames; D010375:Pedigree; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics*; D018626:Retroelements
chemical_list: D017931:DNA Primers; D018094:Receptors, Metabotropic Glutamate; D018626:Retroelements; C104077:metabotropic glutamate receptor type 1