title: A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing.
abstract: We identify a new mammalian cohesin subunit, RAD21-like protein (RAD21L), with sequence similarity to RAD21 and REC8. RAD21L localizes along axial elements in early meiotic prophase, in a manner that is spatiotemporally different to either REC8 or RAD21. Remarkably, RAD21L and REC8 have symmetrical, mutually exclusive localization on the not-yet-synapsed homologues, implying that the cohesin patterning could provide a code for homologue recognition. RAD21 transiently localizes to axial elements after the dissociation of RAD21L and REC8 in late pachytene, a period of recombination repair. Further, we show that the removal of cohesins and synaptonemal complex during late meiotic prophase is promoted by Polo-like kinase 1, which is similar to the mitotic prophase pathway.
authors.affiliation: Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Tokyo 113-0032, Japan.
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Ishiguro
authors.forename: Jihye
authors.initials: J
authors.lastname: Kim
authors.forename: Sally
authors.initials: S
authors.lastname: Fujiyama-Nakamura
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Kato
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002503:Centromere; D002853:Chromatography, Liquid; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D023902:Chromosome Pairing; D020090:Chromosome Segregation; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D004268:DNA-Binding Proteins; D013058:Mass Spectrometry; D008540:Meiosis; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011418:Prophase; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021122:Protein Subunits; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D013573:Synaptonemal Complex / Q000378:metabolism; D000097722:Cohesins; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D017346:Protein Serine-Threonine Kinases; D021122:Protein Subunits; D011518:Proto-Oncogene Proteins; D000097722:Cohesins; D000097869:Polo-Like Kinase 1; C556316:RAD21L protein, mouse; C465862:Rad21 protein, mouse; C465863:Rec8 protein, mouse