title: Ibuprofen metabolism in the liver and gill of rainbow trout, Oncorhynchus mykiss.
abstract: The presence of pharmaceuticals in the environment has become an important topic of discussion with respect to pharmaceutical absorption, metabolism and elimination in fish. This study investigates the metabolism of ibuprofen by rainbow trout (Oncorhynchus mykiss). In vitro metabolic loss of parent compound was measured in gill and liver S9 and microsomal fractions. Metabolite analysis found 2-hydroxy-ibuprofen as the major metabolite in uninduced S9 fractions. Supplementing S9 fractions with UDPGA did not significantly enhance metabolism. Additionally, assays involving the induction and inhibition of specific CYP isozymes support CYP1A2 as a possible metabolic pathway in fish.
authors.affiliation: Department of Biological Sciences, University of North Texas, 1704 West Mulberry, Room 215, Denton, TX 76208, USA.
authors.forename: C F
authors.initials: CF
authors.lastname: Gomez
authors.forename: L
authors.initials: L
authors.lastname: Constantine
authors.forename: M
authors.initials: M
authors.lastname: Moen
authors.forename: A
authors.initials: A
authors.lastname: Vaz
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Duane B
authors.initials: DB
authors.lastname: Huggett
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005880:Gills / Q000378:metabolism*; D007052:Ibuprofen / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D007052:Ibuprofen