title: Landscape of the SOX2 protein-protein interactome.
abstract: SOX2 is a key gene implicated in maintaining the stemness of embryonic and adult stem cells that appears to re-activate in several human cancers including glioblastoma multiforme. Using immunoprecipitation (IP)/MS/MS, we identified 144 proteins that are putative SOX2 interacting proteins. Of note, SOX2 was found to interact with several heterogeneous nuclear ribonucleoprotein family proteins, including HNRNPA2B1, HNRNPA3, HNRNPC, HNRNPK, HNRNPL, HNRNPM, HNRNPR, HNRNPU, as well as other ribonucleoproteins, DNA repair proteins and helicases. Gene ontology (GO) analysis revealed that the SOX2 interactome was enriched for GO terms GO:0030529 ribonucleoprotein complex and GO:0004386 helicase activity. These findings indicate that SOX2 associates with the heterogeneous nuclear ribonucleoprotein complex, suggesting a possible role for SOX2 in post-transcriptional regulation in addition to its function as a transcription factor.
authors.affiliation: Swedish Neuroscience Institute, Swedish Medical Center, Seattle, WA, USA.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Fang
authors.forename: Jae-Geun
authors.initials: JG
authors.lastname: Yoon
authors.forename: Lisha
authors.initials: L
authors.lastname: Li
authors.forename: Yihsuan S
authors.initials: YS
authors.lastname: Tsai
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: Gregory
authors.initials: G
authors.lastname: Foltz
authors.forename: Biaoyang
authors.initials: B
authors.lastname: Lin
mesh_terms: D000328:Adult; D000818:Animals; D001665:Binding Sites; D045744:Cell Line, Tumor; D004265:DNA Helicases / Q000378:metabolism; D004260:DNA Repair / Q000502:physiology; D053595:Embryonic Stem Cells / Q000502:physiology; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D051379:Mice; D009380:Neoplasms, Nerve Tissue / Q000235:genetics / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000502:physiology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012323:RNA Processing, Post-Transcriptional; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012261:Ribonucleoproteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D004265:DNA Helicases