title: Evaluation of oxidative stress biomarkers in Zosterisessor ophiocephalus from the Venice Lagoon, Italy.
abstract: Several studies carried out in the last years have demonstrated the presence of a wide range of contaminants in some areas of the Venice Lagoon. Many of these contaminants are able to drive free radical reactions, which lead to oxidative stress and can potentially affect fish health. In the present study, oxidative stress biomarkers were examined in three different sites (Porto Marghera, Val di Brenta and Caroman) of the Venice Lagoon and their levels monitored in Zosterisessor ophiocephalus, one of the most common fish species present in the lagoon. Schmorl's staining revealed the presence of melanomacrophage centres in spleen and head kidney, and the highest number of melanomacrophage centres was observed in the animals sampled at the Porto Marghera (Porto Marghera vs Val di brenta and Caroman: p<0.01). The cellular localization of HNE and NT, investigated through an immunohistochemical approach, showed that immunopositivity was mainly localized in melanomacrophage centres of spleen and kidney. It is relevant that the animals of the detoxified control group did not exhibit any immunoreactivity. By Western blot, the antibodies against HNE and NT recognized in the liver polypeptides damaged by oxidative stress with molecular weights under 66kDa. Comparing the relative densities, animals from the Val di Brenta site exhibited the lowest levels of HNE adducts (p<0.05), whereas animals from the Porto Marghera site exhibited the highest levels of NT adducts (p<0.05). MDA levels, measured spectrophotometrically by TBARS assay did not exhibit any statistical difference among sites.
authors.affiliation: Department of Experimental Veterinary Sciences, Università degli Studi di Padova, Agripolis Viale dell'Università 16, Legnaro (PD), Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pascoli
authors.forename: Elena
authors.initials: E
authors.lastname: Negrato
authors.forename: Alessia
authors.initials: A
authors.lastname: Di Giancamillo
authors.forename: Daniela
authors.initials: D
authors.lastname: Bertotto
authors.forename: Cinzia
authors.initials: C
authors.lastname: Domeneghini
authors.forename: Claudia
authors.initials: C
authors.lastname: Simontacchi
authors.forename: Franco
authors.initials: F
authors.lastname: Mutinelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Radaelli
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D018736:DNA Adducts / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods / Q000706:statistics & numerical data*; D004785:Environmental Pollutants / Q000633:toxicity*; D005609:Free Radicals / Q000633:toxicity*; D007150:Immunohistochemistry; D007558:Italy; D007668:Kidney / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010473:Perciformes / Q000378:metabolism / Q000502:physiology*; D012623:Seawater; D013154:Spleen / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D004785:Environmental Pollutants; D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances