title: Nocardia artemisiae sp. nov., an endophytic actinobacterium isolated from a surface-sterilized stem of Artemisia annua L.
abstract: A novel actinobacterium, designated YIM 65623(T), was isolated from a surface-sterilized stem of Artemisia annua L. Strain YIM 65623(T) had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus Nocardia. Growth occurred with 0-7% (w/v) NaCl (optimum 0-3%), at pH 5.0-9.0 (optimum pH 6.0) and at 10-37 °C (optimum 20-28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623(T) constituted a distinct sublineage within the genus Nocardia and displayed 94.1-98.2% sequence similarity to members of established species in the genus Nocardia. However, DNA-DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623(T) could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623(T) be classified as a representative of a novel species, Nocardia artemisiae sp. nov. The type strain is YIM 65623(T) (=DSM 45379(T) =CCTCC AA 209038(T)).
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Guo-Zhen
authors.initials: GZ
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization CAS, RNAH Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Yong
authors.initials: WY
authors.lastname: Zhu
authors.affiliation: DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Xu
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization CAS, RNAH Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D031172:Artemisia annua / Q000382:microbiology*; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009615:Nocardia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D018547:Plant Stems / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride