title: Down-regulation of the Wnt, estrogen receptor, insulin-like growth factor-I, and bone morphogenetic protein pathways in osteoblasts from rats with chronic spinal cord injury.
abstract: OBJECTIVE
  To investigate the anabolic response of osteoblasts to chronic spinal cord injury and to identify potential signaling pathways that are associated with the osteogenic response after spinal cord injury by using in-house microarray analyses in osteoblasts.
  
  
  METHODS
  Ten young male Sprague-Dawley rats were randomized into spinal cord injury (SCI) and SHAM groups. The tibiae were assessed for DXA and bone histomorphometry, and osteoblasts from femora were used for microarray analysis.
  
  
  RESULTS
  SCI rats showed lower BMD and deteriorated microstructure in the proximal tibiae as compared with SHAM rats. The Wnt, BMP/TGF, estrogen receptor (ER), and IGF-I pathways were down-regulated in osteoblasts from spinal cord-injured rats.
  
  
  CONCLUSIONS
  Down-regulation of the Wnt, BMP/TGF, ER, and growth hormone/IGF-I pathways is associated with decreased bone formation after spinal cord injury.
authors.affiliation: Department of Orthopaedic Surgery, Xinhua Hospital, 1665 Kongjiang Road, Shanghai 200092, Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Sheng-Dan
authors.initials: SD
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Lei-Sheng
authors.initials: LS
authors.lastname: Jiang
authors.forename: Li-Yang
authors.initials: LY
authors.lastname: Dai
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019485:Bone Morphogenetic Proteins; D011960:Receptors, Estrogen; D051153:Wnt Proteins; D007334:Insulin-Like Growth Factor I