title: RepA-WH1 prionoid: a synthetic amyloid proteinopathy in a minimalist host.
abstract: The intricate complexity, at the molecular and cellular levels, of the processes leading to the development of amyloid proteinopathies is somehow counterbalanced by their common, universal structural basis. The later has fueled the quest for suitable model systems to study protein amyloidosis under quasi-physiological conditions in vitro and in simpler organisms in vivo. Yeast prions have provided several of such model systems, yielding invaluable insights on amyloid structure, dynamics and transmission. However, yeast prions, unlike mammalian PrP, do not elicit any proteinopathy. We have recently reported that engineering RepA-WH1, a bacterial DNA-toggled protein conformational switch (dWH1 → mWH1) sharing some analogies with nucleic acid-promoted PrPC → PrPSc replication, enables control on protein amyloidogenesis in vitro. Furthermore, RepA-WH1 gives way to a non-infectious, vertically-transmissible (from mother to daughter cells) amyloid proteinopathy in Escherichia coli. RepA-WH1 amyloid aggregates efficiently promote aging in bacteria, which exhibit a drastic lengthening in generation time, a limited number of division cycles and reduced fitness. The RepA-WH1 prionoid opens a direct means to untangle the general pathway(s) for protein amyloidosis in a host with reduced genome and proteome.
authors.affiliation: Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas - CSIC, C/ Ramiro de Maeztu, Madrid, Spain. rgiraldo@cib.csic.es
authors.forename: Rafael
authors.initials: R
authors.lastname: Giraldo
authors.forename: Susana
authors.initials: S
authors.lastname: Moreno-Díaz de la Espina
authors.forename: M Elena
authors.initials: ME
authors.lastname: Fernández-Tresguerres
authors.forename: Fátima
authors.initials: F
authors.lastname: Gasset-Rosa
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D000686:Amyloidosis; D004265:DNA Helicases / Q000737:chemistry; D008854:Microscopy, Electron; D011328:Prions / Q000737:chemistry; D017433:Protein Structure, Secondary; D015534:Trans-Activators / Q000737:chemistry
chemical_list: D000682:Amyloid; D011328:Prions; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases