title: Trinucleotide repeat sequence-based PCR as a potential approach for genotyping Mycobacterium gordonae strains.
abstract: Diseases that are caused by non-tuberculous mycobacteria (NTM) continue to pose difficult clinical problems, and the epidemiological aspect of NTM-caused diseases is of great importance. In the case of Mycobacterium gordonae there is no adequate genotyping scheme. Here we present a potential rapid and reproducible genetic assay that uses trinucleotide repeat sequence-based PCR (TRS-PCR) for genotyping M. gordonae. The proposed method constitutes a useful single-primer PCR screen for genotyping this species. Among 10 TRS-containing primers, after applying (CAC)₄-based PCR to 36 strains of M. gordonae, we found a discriminatory index of 0.975. The accuracy of this analysis was supported by a reasonable reproducibility of 92%. These results were compared with the Enterobacterial Repetitive Intergenic Consensus Sequences (ERIC)-PCR typing scheme which had lower discriminatory index of 0.93 and its reproducibility was only 86.3%.
authors.affiliation: Institute of Medical Biology PAS, 106 Lodowa Str., 93-232 Lodz, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Wojtasik
authors.forename: Marta
authors.initials: M
authors.lastname: Majchrzak
authors.forename: Wioletta
authors.initials: W
authors.lastname: Adamus-Bialek
authors.forename: Ewa
authors.initials: E
authors.lastname: Augustynowicz-Kopec
authors.forename: Zofia
authors.initials: Z
authors.lastname: Zwolska
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Dziadek
authors.forename: Pawel
authors.initials: P
authors.lastname: Parniewski
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018911:Trinucleotide Repeats
chemical_list: D004269:DNA, Bacterial