title: Challenging the golden standard in defining donor-specific antibodies: does the solid phase assay meet the expectations?
abstract: The purpose of the study was to compare three different methods defining donor-specific antibodies (DSA): complement-dependent cytotoxicity (CDC), the flow cytometry method (FCM), and a special for that purpose commercially available Luminex-based solid phase assay (SPA). A panel of human monoclonal antibodies (HuMabs) with well-defined human leukocyte antigen (HLA) specificities was used as antibody source and single HLA antigen expressing cell lines (SAL) were used as targets. Two methods yielded identical results (CDC and FCM). However, the SPA, the method by which solubilized HLA molecules from the SAL are captured by microspheres, showed two additional reactions which could not be explained, neither by the epitope recognized by the HuMab nor by the widely accepted sensitivity of the SPA methodology. These unexplained results suggest that by capturing solubilized HLA molecules on microspheres, conformational changes might occur. Positive results obtained by similar Luminex-based microsphere methods should be therefore taken with caution and the 'recognized' HLA antigens should not automatically be considered as unacceptable for transplantation.
authors.affiliation: Department of Immunohaematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Y M
authors.initials: YM
authors.lastname: Zoet
authors.forename: S H
authors.initials: SH
authors.lastname: Brand-Schaaf
authors.forename: D L
authors.initials: DL
authors.lastname: Roelen
authors.forename: A
authors.initials: A
authors.lastname: Mulder
authors.forename: F H J
authors.initials: FH
authors.lastname: Claas
authors.forename: I I N
authors.initials: II
authors.lastname: Doxiadis
mesh_terms: D000906:Antibodies / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001782:Blood Donors; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods / Q000592:standards; D005434:Flow Cytometry / Q000379:methods; D006650:Histocompatibility Testing / Q000379:methods / Q000592:standards; D006801:Humans; D020014:K562 Cells; D012015:Reference Standards; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000592:standards* / Q000706:statistics & numerical data*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal