title: Longer storage of dialyzers increases elution of poly(N-vinyl-2-pyrrolidone) from polysulfone-group dialysis membranes.
abstract: The objective of this study was to evaluate the effect of protracted storage of dialyzers on the amount of poly(N-vinyl-2-pyrrolidone) (PVP) eluted from polysulfone-group dialysis membranes. We tested five dialysis membranes: APS-15SA (Asahi Kasei Kuraray, wet), CX-1.6U (Toray, moist), FX140 (Fresenius, dry), PES-15Sα (Nipro, dry), and FDX-150GW (Nikkiso, wet). Each dialyzer was stored for 1, 3, 14, and 18 months after sterilization. The dialysis-fluid side compartment was primed with reverse osmosis (RO) water at 500 mL/min for 5 min at 310 K. The blood side compartment was primed with RO water at 200 mL/min for 5 min at 310 K. Finally, 1 L RO water was circulated through the blood side compartment at 200 mL/min for 4 h at 310 K. Eluted PVP was determined by use of the iodine method, using 0.02 N: iodine solution. PVP was mainly eluted from wet-type dialyzers during priming. Thus, the standard 5 min priming of the wet-type dialyzer according to the maker manual inhibits PVP elution during circulation. PVP was eluted in the dialysis-fluid side of the moist-type dialyzer during priming but no PVP was eluted in the blood side. PVP was mainly eluted from dry-type dialyzers during circulation. We recommend more than the standard 5 min priming, particularly for dry-type dialyzers stored for protracted periods, because 5 min insufficient to inhibit PVP elution during circulation.
authors.affiliation: Sakai Laboratory, Department of Chemical Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan. namex@fuji.waseda.jp
authors.forename: Koki
authors.initials: K
authors.lastname: Namekawa
authors.forename: Ami
authors.initials: A
authors.lastname: Kaneko
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Sakai
authors.forename: Satoru
authors.initials: S
authors.lastname: Kunikata
authors.forename: Masato
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D008567:Membranes, Artificial; D011108:Polymers; D006435:Renal Dialysis / Q000379:methods*; D013242:Sterilization; D013450:Sulfones; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700