title: MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia.
abstract: Elevated levels of microRNA miR-155 represent a candidate pathogenic factor in chronic B-lymphocytic leukemia (B-CLL). In this study, we present evidence that MYB (v-myb myeloblastosis viral oncogene homolog) is overexpressed in a subset of B-CLL patients. MYB physically associates with the promoter of miR-155 host gene (MIR155HG, also known as BIC, B-cell integration cluster) and stimulates its transcription. This coincides with the hypermethylated histone H3K4 residue and spread hyperacetylation of H3K9 at MIR155HG promoter. Our data provide evidence of oncogenic activities of MYB in B-CLL that include its stimulatory role in MIR155HG transcription.
authors.affiliation: First Faculty of Medicine and Center of Experimental Hematology, Charles University in Prague, U Nemocnice 5, Prague, Czech Republic.
authors.forename: Karin
authors.initials: K
authors.lastname: Vargova
authors.forename: Nikola
authors.initials: N
authors.lastname: Curik
authors.forename: Pavel
authors.initials: P
authors.lastname: Burda
authors.forename: Petra
authors.initials: P
authors.lastname: Basova
authors.forename: Vojtech
authors.initials: V
authors.lastname: Kulvait
authors.forename: Vit
authors.initials: V
authors.lastname: Pospisil
authors.forename: Filipp
authors.initials: F
authors.lastname: Savvulidi
authors.forename: Juraj
authors.initials: J
authors.lastname: Kokavec
authors.forename: Emanuel
authors.initials: E
authors.lastname: Necas
authors.forename: Adela
authors.initials: A
authors.lastname: Berkova
authors.forename: Petra
authors.initials: P
authors.lastname: Obrtlikova
authors.forename: Josef
authors.initials: J
authors.lastname: Karban
authors.forename: Marek
authors.initials: M
authors.lastname: Mraz
authors.forename: Sarka
authors.initials: S
authors.lastname: Pospisilova
authors.forename: Jiri
authors.initials: J
authors.lastname: Mayer
authors.forename: Marek
authors.initials: M
authors.lastname: Trneny
authors.forename: Jiri
authors.initials: J
authors.lastname: Zavadil
authors.forename: Tomas
authors.initials: T
authors.lastname: Stopka
mesh_terms: D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006367:HeLa Cells; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D020626:Oncogene Proteins v-myb / Q000378:metabolism / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic / Q000502:physiology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D002843:Chromatin; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D020626:Oncogene Proteins v-myb