title: Lysate microarrays enable high-throughput, quantitative investigations of cellular signaling.
abstract: Lysate microarrays (reverse-phase protein arrays) hold great promise as a tool for systems-level investigations of signaling and multiplexed analyses of disease biomarkers. To date, however, widespread use of this technology has been limited by questions concerning data quality and the specificity of detection reagents. To address these concerns, we developed a strategy to identify high-quality reagents for use with lysate microarrays. In total, we tested 383 antibodies for their ability to quantify changes in protein abundance or modification in 20 biological contexts across 17 cell lines. Antibodies yielding significant differences in signal were further evaluated by immunoblotting and 82 passed our rigorous criteria. The large-scale data set from our screen revealed that cell fate decisions are encoded not just by the identities of proteins that are activated, but by differences in their signaling dynamics as well. Overall, our list of validated antibodies and associated protocols establish lysate microarrays as a robust tool for systems biology.
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, Massachusetts 02138, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Sevecka
authors.forename: Alejandro
authors.initials: A
authors.lastname: Wolf-Yadlin
authors.forename: Gavin
authors.initials: G
authors.lastname: MacBeath
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D003429:Cross Reactions; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000276:immunology / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction
chemical_list: D000906:Antibodies; D000941:Antigens; D015415:Biomarkers; D047908:Intracellular Signaling Peptides and Proteins