title: Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media.
abstract: Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of α-ketoglutarate, which is known to be released by several cell types, decreased the levels of H(2)O(2), and the α-ketoglutarate was depleted and converted to succinate. These observations could account for previous reports of the protective effects of α-ketoglutarate in promoting the growth of cells in culture, and may contribute to explaining some of the variability in the literature in reported rates of H(2)O(2) production from autoxidisable compounds in cell culture systems.
authors.affiliation: Department of Biochemistry, National University of Singapore, Singapore.
authors.forename: Lee Hua
authors.initials: LH
authors.lastname: Long
authors.forename: Barry
authors.initials: B
authors.lastname: Halliwell
mesh_terms: D000818:Animals; D016477:Artifacts; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D019802:Succinic Acid / Q000737:chemistry / Q000378:metabolism
chemical_list: D003470:Culture Media; D007656:Ketoglutaric Acids; D002392:Catechin; D019802:Succinic Acid; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D001205:Ascorbic Acid