title: Characterization of porcine multipotent stem/stromal cells derived from skin, adipose, and ovarian tissues and their differentiation in vitro into putative oocyte-like cells.
abstract: The present study evaluated the alkaline phosphatase activity, cell cycle stage, expression of markers and early transcriptional factors, and in vitro differentiation into selected cell lineages of porcine stem/stromal cells (SCs) isolated from skin (SSCs), adipose, and ovarian (OSCs) tissues. Skin and adipose SCs were isolated from a 6-month-old miniature pig, whereas OSCs were isolated from a newly born piglet. Isolated cells exhibited fibroblast-like cell population with significant renewal capacity and formed colonies by cells out-growth. All cells were positive for alkaline phosphatase activity and showed a relatively lower population at G0/G1 phase of the cell cycle. SCs derived from all tissues were strongly positive for cell surface markers, such as CD29, CD44, CD90, and vimentin. Further, relatively lower expression of cytokeratin and immunophenotype markers, such as major histocompatibility complex II (MHCII) and swine leukocyte antigen (SLA), was also observed. SCs derived from all tissues positively expressed the transcription factors, such as Oct-3/4, Nanog, and Sox-2. After induction, all SCs successfully differentiated into osteocytes and adipocytes and expressed the lineage specific marker genes. Further, cells from all tissues exhibited their potential for in vitro oogenesis with morphological changes and expression of markers during the germ-cell formation, namely Oct-4, growth differentiation factor 9b, c-Mos, Vasa, deleted in azoospermia-like gene, zona pellucida C, and follicle stimulating hormone receptor. Apart from basic features and selected lineage potential among all types of cells, OSCs possessed a greater ability to differentiate into the germ cell lineage in vitro.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Song
authors.forename: Basavarajappa Mohana
authors.initials: BM
authors.lastname: Kumar
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kang
authors.forename: Yeon-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Kim
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Ock
authors.forename: Sung-Lim
authors.initials: SL
authors.lastname: Lee
authors.forename: Byeong-Gyun
authors.initials: BG
authors.lastname: Jeon
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D009866:Oogenesis; D010053:Ovary / Q000166:cytology; D012867:Skin / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D013552:Swine
chemical_list: D015415:Biomarkers; D000469:Alkaline Phosphatase