title: Bifidobacterium lactis attenuates onset of inflammation in a murine model of colitis.
abstract: OBJECTIVE
  To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis (B. lactis) in an adoptive transfer model of colitis.
  
  
  METHODS
  Donor and recipient mice received either B. lactis or bacterial culture medium as control (deMan Rogosa Sharpe) in drinking water for one week prior to transfer of a mix of naive and regulatory T cells until sacrifice.
  
  
  RESULTS
  All recipient mice developed signs of colonic inflammation, but a significant reduction of weight loss was observed in B. lactis-fed recipient mice compared to control mice. Moreover, a trend toward a diminution of mucosal thickness and attenuated epithelial damage was revealed. Colonic expression of pro-inflammatory and T cell markers was significantly reduced in B. lactis-fed recipient mice compared to controls. Concomitantly, forkhead box protein 3, a marker of regulatory T cells, was significantly up-regulated by B. lactis.
  
  
  CONCLUSIONS
  Daily oral administration of B. lactis was able to reduce inflammatory and T cells mediators and to promote regulatory T cells specific markers in a mouse model of colitis.
authors.affiliation: Department of Nutrition and Health, Nestlé Research Centre, Vers-chez-les-Blanc, Lausanne 26, Switzerland. david.philippe@rdls.nestle.com
authors.forename: David
authors.initials: D
authors.lastname: Philippe
authors.forename: Laurent
authors.initials: L
authors.lastname: Favre
authors.forename: Francis
authors.initials: F
authors.lastname: Foata
authors.forename: Oskar
authors.initials: O
authors.lastname: Adolfsson
authors.forename: Genevieve
authors.initials: G
authors.lastname: Perruisseau-Carrier
authors.forename: Karine
authors.initials: K
authors.lastname: Vidal
authors.forename: Gloria
authors.initials: G
authors.lastname: Reuteler
authors.forename: Johanna
authors.initials: J
authors.lastname: Dayer-Schneider
authors.forename: Christoph
authors.initials: C
authors.lastname: Mueller
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Blum
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001644:Bifidobacterium / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D001835:Body Weight; D003092:Colitis / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology*; D004195:Disease Models, Animal; D005243:Feces / Q000382:microbiology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000008:administration & dosage / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015415:Biomarkers