title: Evaluation of multiplex immunoassays, used for determination of adiponectin, resistin, leptin, and ghrelin from human blood samples, in comparison to ELISA assays.
abstract: BACKGROUND
  Many different multiplex biomarker immunoassays based on Luminex®-technology have been developed during recent years. We have evaluated the performance of two multiplex immunoassays for determination of adiponectin, resistin, ghrelin and leptin in comparison to corresponding, conventional ELISA assays.
  
  
  METHODS
  Human serum or plasma samples were analysed by commercially available multiplex and ELISA immunoassays manufactured by Millipore Corp.
  
  
  RESULTS
  The correlation between tested multiplex and ELISA immunoassays was good, r > 0.9 for all analytes. The agreement between the methods was acceptable but there were differences in analytical levels. Intra- and inter-assay variation was comparable between both assays. The coefficient of variation for all analytes, independent of method, was ≤15% and for most of them <10%.
  
  
  CONCLUSIONS
  The performance of the tested multiplex assays was reasonable and they can be considered as valid options to the conventional ELISA assays. However, results obtained with the two different techniques are not necessarily interchangeable due to differences in the concentration levels.
authors.affiliation: National Institute for Health and Welfare, Department of Chronic Disease Prevention, Turku, Finland. britt-marie.loo@thl.fi
authors.forename: Britt-Marie
authors.initials: BM
authors.lastname: Loo
authors.forename: Jukka
authors.initials: J
authors.lastname: Marniemi
authors.forename: Antti
authors.initials: A
authors.lastname: Jula
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054439:Ghrelin / Q000097:blood*; D006801:Humans; D007118:Immunoassay; D020738:Leptin / Q000097:blood*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D010949:Plasma; D052243:Resistin / Q000097:blood*; D044967:Serum
chemical_list: D052242:Adiponectin; D054439:Ghrelin; D020738:Leptin; D052243:Resistin