title: Design and synthesis of Raman reporter molecules for tissue imaging by immuno-SERS microscopy.
abstract: The design and synthesis of Raman reporter molecules comprising olefin or alkyne moieties with strong and characteristic vibrational Raman bands is presented. Chemisorption onto the surface of colloidal Au/Ag shells yields a self-assembled monolayer. Hydrophilic stabilization of such SERS labels can be achieved by short terminal ethylene glycol units attached to the Raman reporter. Encapsulation by silica with subsequent functionalization of the glass surface allows the conjugation to biomolecules such as antibodies. We demonstrate the use of SERS-labeled antibodies for tissue imaging of the tumor suppressor p63 in prostate biopsies.
authors.affiliation: Department of Physics, University of Osnabrück, Barbarastr. 7, 49069 Osnabrück, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Schütz
authors.forename: Christian I
authors.initials: CI
authors.lastname: Müller
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
authors.forename: Christoph
authors.initials: C
authors.lastname: Lambert
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schlücker
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry; D019855:Ethylene Glycol / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D011467:Prostate / Q000473:pathology*; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000480:Alkynes; D000906:Antibodies; C081876:CKAP4 protein, human; D008565:Membrane Proteins; D012834:Silver; D006046:Gold; D012822:Silicon Dioxide; D019855:Ethylene Glycol