title: The Evi1, microRNA-143, K-Ras axis in colon cancer.
abstract: MicroRNA profiling of diseased/non-diseased tissue has identified expression signatures associated with a wide range of pathogenic conditions including malignancy. For example, colon cancer is associated with the under expression of miRNA-143 yet the molecular etiology of under expression is unknown. The K-Ras oncogene is a target of miRNA-143. Here, we show that the ecotropic viral integration site 1 oncoprotein (Evi1) is a transcriptional suppressor of the miRNA-143 gene. We find an indirect relationship between miRNA-143 and Evi1 expression. A complex molecular axis linking Evi1, miRNA-143 is operational in human colon cancer.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Miriam and Rhode Island Hospitals, Warren Alpert Medical School of Brown University, Providence, RI 02903, USA.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Gao
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Tang
authors.forename: Lynne D
authors.initials: LD
authors.lastname: Tucker
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Tarwater
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Quesenberry
authors.forename: Isidore
authors.initials: I
authors.lastname: Rigoutsos
authors.forename: Bharat
authors.initials: B
authors.lastname: Ramratnam
mesh_terms: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D003110:Colonic Neoplasms / Q000378:metabolism*; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D000074008:MDS1 and EVI1 Complex Locus Protein; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics / Q000378:metabolism*; D011519:Proto-Oncogenes / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D014015:Tissue Banks; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D000074008:MDS1 and EVI1 Complex Locus Protein; C063575:MECOM protein, human; C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D014157:Transcription Factors; C487931:mirnlet7 microRNA, human; D016283:Proto-Oncogene Proteins p21(ras)