title: Simultaneous determination of ciclesonide and its active metabolite desisobutyryl-ciclesonide in human plasma by LC-APCI-MS/MS: application to pharmacokinetic study in healthy Chinese volunteers.
abstract: A sensitive and highly selective liquid chromatography tandem mass spectrometric (LC/MS/MS) method was developed and validated for the determination of ciclesonide (CIC) and its active metabolite, desisobutyryl-ciclesonide (des-CIC), in human plasma. Plasma samples were extracted using methyl tert-butyl ether with mifepristone as an internal standard (IS). Separation was carried out on a C(18) column using a mixture of 0.1% formic acid solution and methanol as the mobile phase with linear gradient elution. The detection was operated with positive atmospheric pressure chemical ionization (APCI) by selective multiple reaction monitoring (SRM). The chief benefit of the present method was the high sensitivity, with the lower limit of quantification (LLOQ) as low as 10pg/mL and the linearity ranging from 10 to 10,000pg/mL for both CIC and des-CIC. The method was fully validated and successfully applied to determine CIC and des-CIC simultaneously in human plasma and proved to be suitable for phase I clinical pharmacokinetic study of inhaled ciclesonide in healthy Chinese volunteers.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, People's Republic of China.
authors.forename: Meng-xiang
authors.initials: MX
authors.lastname: Su
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Yong-qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Tai-Jun
authors.initials: TJ
authors.lastname: Hang
mesh_terms: D000328:Adult; D018926:Anti-Allergic Agents / Q000097:blood / Q000493:pharmacokinetics*; D001711:Biotransformation; D002138:Calibration; D002681:China; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008832:Microchemistry / Q000379:methods; D011282:Pregnenediones / Q000097:blood* / Q000493:pharmacokinetics*; D011355:Prodrugs / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D018926:Anti-Allergic Agents; D011282:Pregnenediones; D011355:Prodrugs; C494355:desisobutyrylciclesonide; C120481:ciclesonide