title: ATP photosynthetic vesicles for light-driven bioprocesses.
abstract: We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Nada, Kobe, Japan.
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Rie
authors.initials: R
authors.lastname: Suzuki
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masasuke
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kuniki
authors.initials: K
authors.lastname: Kino
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D025243:Bacterial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006216:Halobacteriaceae / Q000235:genetics / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D025604:Rhodopsins, Microbial / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D025604:Rhodopsins, Microbial; D000255:Adenosine Triphosphate; D006593:Hexokinase; D025243:Bacterial Proton-Translocating ATPases