title: Evaluation of three swab transport systems for the maintenance of clinically important bacteria in simulated mono- and polymicrobial samples.
abstract: In this study, three swab transport systems were evaluated: M40 Transystem, Amies broth with a relatively new type of swab (both Copan Diagnostics, Corona, CA, USA), and SSI transportmedium (Statens Serum Institut, Copenhagen Denmark). The CLSI M40-A standard procedures and 11 culture collection strains were used. The transport systems were tested at room temperature for holding times of 0, 24, and 48 h, and both mono- and polymicrobial samples were included. After 24 h of simulated transportation, all systems were able to maintain the viability of all organisms tested. SSI transportmedium exhibited the lowest maintaining ability, whereas the two Copan systems were the most growth-promoting system. In polymicrobial samples, this latter feature was a problem. At 48 h, no transport system could maintain the viability of all strains, and the recovery rates differed depending on organism and device. The species most difficult to recover in all the three systems was Neisseria gonorrhoeae. When selecting a swab transport system, consideration must be given to the sample type, the conditions that prevail locally, and the performance in the clinical setting.
authors.affiliation: Department of Medical Sciences/Section of Clinical Bacteriology, Uppsala University, Sweden. eva.tano@akademiska.se
authors.forename: Eva
authors.initials: E
authors.lastname: Tano
authors.forename: Asa
authors.initials: A
authors.lastname: Melhus
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D058491:Bacterial Load; D001431:Bacteriological Techniques / Q000295:instrumentation* / Q000379:methods; D003470:Culture Media; D013293:Enterococcus faecalis / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D006193:Haemophilus influenzae / Q000302:isolation & purification; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D010462:Peptostreptococcus / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013297:Streptococcus pyogenes / Q000302:isolation & purification; D013997:Time Factors; D014186:Transportation
chemical_list: D003470:Culture Media