title: Galactoglucomannan Oligosaccharides (GGMO) from a molasses byproduct of pine ( Pinus taeda ) fiberboard production.
abstract: "Temulose" is the trade name for a water-soluble molasses produced on a large scale (300-400 tonnes per year) as a byproduct of the fiberboard industry. The feedstock for Temulose is predominantly a single species of pine ( Pinus taeda ) grown and harvested in stands in southeastern Texas. Because of the method of production, the molasses was predicted to consist of water-soluble hemicelluloses, mainly arabinoxylan-type and galactoglucomannan-type oligosaccharides, plus minor components of lignin, but no detailed structural study had been reported. The structure and composition of the molasses has now been deduced by a combination of MALDI-TOF mass spectrometry, size exclusion chromatography, proton and (13)C NMR techniques, and classic carbohydrate analysis. Limited acid hydrolysis released a series of galactoglucomannan oligosaccharides (GGMO) that were selectively recovered from the acid-labile arabinogalactan by precipitation with ethanol. The precipitate was named "Temulose brown sugar" because of its appearance, and is shown to consist of GGMO with a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a β-1,4-linked backbone of Man and Glc residues, with occasional α-1,6 branching by single galactosyl units.
authors.affiliation: National Center for Agricultural Utilization Research, U.S. Department of Agriculture , Peoria, Illinois 61604, United States. neil.price@ars.usda.gov
authors.forename: Neil P J
authors.initials: NP
authors.lastname: Price
authors.forename: Trina M
authors.initials: TM
authors.lastname: Hartman
authors.forename: Trevor A
authors.initials: TA
authors.lastname: Faber
authors.forename: Karl E
authors.initials: KE
authors.lastname: Vermillion
authors.forename: George C
authors.initials: GC
authors.lastname: Fahey
mesh_terms: D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis* / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D041603:Pinus taeda / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008351:Mannans; D009005:Monosaccharides; D009844:Oligosaccharides; C443804:galactoglucomannan