title: Agroinoculation of Nicotiana benthamiana with cloned honeysuckle yellow vein virus isolated from Lonicera japonica.
abstract: A new honeysuckle yellow vein geminivirus (HYVV) isolate (2,763 nucleotides) and the HYVV-β satellite (1,227 nucleotides) from Korea were cloned and characterized from symptomatic leaves of Lonicera japonica with a yellow net mosaic appearance. Phylogenetic analysis of HYVV and 13 other begomoviruses revealed that HYVV has the highest nucleotide sequence homology to HYVV-UK2. Grafting challenge of a virus-free L. japonica (scion) on an HYVV-infected L. japonica (stock) resulted in the appearance of typical HYVV disease symptoms on the newly developed leaves of the scion. Two pMon521 plasmids containing 1.3 copies and 2.0 copies of the cloned HYVV isolate, respectively, were infectious and produced disease symptoms in Nicotiana benthamiana after agroinoculation. We conclude that the causal agent of yellow net mosaic in L. japonica is a new Korean isolate of HYVV, based on sequence comparisons, agroinoculation-induced disease symptoms, electron microscopy, and phylogenetic analysis.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: Gunsup
authors.initials: G
authors.lastname: Lee
authors.forename: Sunghan
authors.initials: S
authors.lastname: Kim
authors.forename: Jihye
authors.initials: J
authors.lastname: Jung
authors.forename: Chung-Kyoon
authors.initials: CK
authors.lastname: Auh
authors.forename: Eunseok
authors.initials: E
authors.lastname: Choi
authors.forename: Mooung
authors.initials: M
authors.lastname: Chang
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
mesh_terms: D053839:Begomovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D007723:Korea; D031239:Lonicera / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology
chemical_list: D004279:DNA, Viral