title: Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides.
abstract: Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos. Using MTT assay 12 h IC(50) values calculated were 31.09 ± 16.27 μM and 5.52 ± 1.16 μM for cadmium chloride and mercuric chloride and 59.94 ± 52.30 mg l(-1) and 186.76 ± 77.00 mg l(-1) for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.
authors.affiliation: National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi 682016, India.
authors.forename: Seena
authors.initials: S
authors.lastname: Jose
authors.forename: P
authors.initials: P
authors.lastname: Jayesh
authors.forename: A
authors.initials: A
authors.lastname: Mohandas
authors.forename: Rosamma
authors.initials: R
authors.lastname: Philip
authors.forename: I S
authors.initials: IS
authors.lastname: Bright Singh
mesh_terms: D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity; D002478:Cells, Cultured; D003603:Cytotoxins / Q000633:toxicity; D006434:Hemocytes / Q000187:drug effects*; D008294:Malathion / Q000633:toxicity; D008627:Mercuric Chloride / Q000633:toxicity; D019216:Metals, Heavy / Q000633:toxicity*; D008999:Monocrotophos / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D033561:Penaeidae / Q000187:drug effects*; D010575:Pesticides / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D003603:Cytotoxins; D019216:Metals, Heavy; D009153:Mutagens; D010575:Pesticides; D008627:Mercuric Chloride; D008999:Monocrotophos; D019256:Cadmium Chloride; D008294:Malathion