title: New isoflavonoid glycosides and related constituents from astragali radix ( Astragalus membranaceus ) and their inhibitory activity on nitric oxide production.
abstract: Twenty-four secondary metabolites, including 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (-)-methylinissolin 3-O-β-d-(6'-acetyl)-glucoside (1), (-)-methylinissolin 3-O-β-d-{6'-[(E)-but-2-enoyl]}-glucoside (2), and calycosin 7-O-β-d-(6''-acetyl)-glucoside (3) have been identified as new compounds on the basis of spectroscopic analysis; (-)-methylinissolin 3-O-β-d-glucoside (4) was isolated from the natural products for the first time. The nitric oxide (NO) production inhibitory activity of the major compounds has been assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. To identify A. membranaceus, a fingerprint method was developed by using a high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method. Furthermore, characteristic peaks for the 11 major compounds in the chromatogram were unambiguously confirmed.
authors.affiliation: National Research Institute of Chinese Medicine , Taipei 112, Taiwan, Republic of China.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Hui-Kang
authors.initials: HK
authors.lastname: Liu
authors.forename: Ping-Chun
authors.initials: PC
authors.lastname: Hsiao
authors.forename: Li-Ming Yang
authors.initials: LM
authors.lastname: Kuo
authors.forename: I-Jung
authors.initials: IJ
authors.lastname: Lee
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
authors.forename: Wen-Fei
authors.initials: WF
authors.lastname: Chiou
authors.forename: Yao-Haur
authors.initials: YH
authors.lastname: Kuo
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry*; D002460:Cell Line; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D005419:Flavonoids; D006027:Glycosides; D008070:Lipopolysaccharides; D009569:Nitric Oxide