title: Phylogenetic analysis of the E2 gene of classical swine fever virus from the Guangxi Province of southern China.
abstract: In this study, suspected classical swine fever (CSF) samples from the Guangxi Province of China were obtained from pigs with acute CSF, aborted fetuses, newborn pigs that died at 1-2 days of age, tonsils of healthy pigs, and leukocytes of immunized sows during 2001-2009. About 92 of 775 samples were found to be positive by RT-PCR, and 41 isolates were obtained. Phylogenetic analysis was performed on the 31 isolates by sequencing the E2 gene, and the isolates were found to cluster into two groups: (1) isolates from aborted fetuses (except GXGZ02), deceased newborn baby pigs, tonsils of healthy pigs, and leukocytes of immunized sows belonged to group 1.1, along with vaccine strain, HCLV, and standard virulent strain, Shimen, of China, and (2) 20 isolates from pigs with acute CSF belonged to group 2.1, 13 of which were clustered into subgroup 2.1b with isolates from other provinces of China, and 7 of which were clustered into subgroup 2.1a with isolates from Italy and Germany.
authors.affiliation: College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning, China. trluo@tom.com
authors.forename: Ting Rong
authors.initials: TR
authors.lastname: Luo
authors.forename: Su-Huan
authors.initials: SH
authors.lastname: Liao
authors.forename: Xian-Shi
authors.initials: XS
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Yuan
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liang
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Meng
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D006691:Classical Swine Fever / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus