title: Participation of BKCa2+ and KATP potassium ion channels in the contractility of human term pregnant myometrium in in vitro conditions.
abstract: OBJECTIVE
  The aim of this study was to assess the participation of ligand-sensitive potassium large conductance calcium-activated ion channels (BK(Ca2+) ) and adenosine triphosphate (ATP)-sensitive potassium ion channels (K(ATP) ) using its openers (NS1619 and pinacidil) in the contractility of human term pregnant myometrium in in vitro conditions.
  
  
  METHODS
  Human myometrium tissue samples were collected from term pregnant laboring women who had to undergo cesarean section. The contractility of myometrium was induced by the application of oxytocin into the organ bath. Myometrial strips were incubated with the opener of BK(Ca2+) potassium ion channels NS1619 and its antagonist tetraethylammonium or with the opener of K(ATP) potassium ion channels pinacidil and its antagonist glibenclamide.
  
  
  RESULTS
  K(ATP) potassium ion channel's opener pinacidil significantly decreased amplitude of myometrial contractions (P < 0.05) as well as frequency of myometrial contractions (P < 0.05) provoked by oxytocin in human term pregnant myometrium in in vitro conditions. The inhibition of the human myometrial contractions of pinacidil was significantly antagonized by its specific antagonist glibenclamide (P < 0.05). BK(Ca2+) potassium ion channel's opener NS1619 did not significantly affect the contractile activity of human term pregnant myometrium induced by the application of oxytocin in in vitro conditions.
  
  
  CONCLUSIONS
  In our experimental study we found that the participation of BK(Ca2+) and K(ATP) potassium ion channels in the contractility of human term pregnant myometrium in labor is probably different.
authors.affiliation: Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia. vsadlonova@jfmed.uniba.sk
authors.forename: Vladimira
authors.initials: V
authors.lastname: Sadlonova
authors.forename: Sona
authors.initials: S
authors.lastname: Franova
authors.forename: Karol
authors.initials: K
authors.lastname: Dokus
authors.forename: Frantisek
authors.initials: F
authors.lastname: Janicek
authors.forename: Jozef
authors.initials: J
authors.lastname: Visnovsky
authors.forename: Jurina
authors.initials: J
authors.lastname: Sadlonova
mesh_terms: D001562:Benzimidazoles / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D054086:KATP Channels / Q000187:drug effects / Q000502:physiology*; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000187:drug effects / Q000502:physiology*; D049990:Membrane Transport Modulators / Q000494:pharmacology; D009215:Myometrium / Q000502:physiology*; D010121:Oxytocin; D020110:Pinacidil / Q000494:pharmacology; D011247:Pregnancy; D014590:Uterine Contraction / Q000187:drug effects / Q000502:physiology*
chemical_list: D001562:Benzimidazoles; D054086:KATP Channels; D051036:Large-Conductance Calcium-Activated Potassium Channels; D049990:Membrane Transport Modulators; C086491:NS 1619; D010121:Oxytocin; D020110:Pinacidil