title: Identification of oxidized serum albumin in the cerebrospinal fluid of ischaemic stroke patients.
abstract: OBJECTIVE
  Extensive evidence has shown that oxidative stress mediates neuronal death in animal models of hypoxic-ischaemia. Brain biomarkers of oxidative stress need to be identified in order to better understand and treat brain damage in human stroke patients. The present study was conducted to identify potential target proteins of oxidative stress in the cerebrospinal fluid (CSF) of stroke patients with acute ischaemic brain injury.
  
  
  METHODS
  We performed two-dimensional polyacrylamide gel electrophoresis to separate protein samples obtained from the CSF of control and stroke patients. To determine protein oxidation levels, oxyblot was then used to detect protein carbonyls that were determined by formation of a stable 2,4-dinitrophenylhydrazine (DNP) product using an anti-DNP antibody.
  
  
  RESULTS
  We found that oxidation of serum albumin was increased in the CSF from stroke patients as well as rats who underwent permanent middle cerebral artery occlusion (6.5%, 23%, respectively). In stroke patients, oxidized albumin levels correlated to neurologic indications.
  
  
  CONCLUSIONS
  The present study suggests that oxidized albumin in CSF can be utilized as an oxidative stress marker in human stroke patients.
authors.affiliation: Research Institute for Neural Science and Technology, Ajou University School of Medicine, Suwon, South Korea.
authors.forename: G J
authors.initials: GJ
authors.lastname: Moon
authors.forename: D H
authors.initials: DH
authors.lastname: Shin
authors.forename: D S
authors.initials: DS
authors.lastname: Im
authors.forename: O Y
authors.initials: OY
authors.lastname: Bang
authors.forename: H S
authors.initials: HS
authors.lastname: Nam
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: I S
authors.initials: IS
authors.lastname: Joo
authors.forename: K
authors.initials: K
authors.lastname: Huh
authors.forename: B J
authors.initials: BJ
authors.lastname: Gwag
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000134:cerebrospinal fluid*; D020521:Stroke / Q000134:cerebrospinal fluid*
chemical_list: D015415:Biomarkers; D012709:Serum Albumin