title: [Initial results of a novel technique for sperm retrieval in male infertility due to refractory retrograde ejaculation].
abstract: OBJECTIVE
  To describe and evaluate a novel technique of spermatozoa retrieval from patients suffering from infertility secondary to refractory retrograde ejaculation.
  
  
  METHODS
  Prospective study to compare mobility and vitality of spermatozoa obtained from urine (U) after oral modification of chemical parameter (PH, Osmolarity) versus from endovesical instillation of sterile spermatozoa culture medium before ejaculation (F). Patients were their own controls. Twelve month prospective follow-up was achieved to document the results of assisted procreation.
  
  
  RESULTS
  Eight patients were included and mobility and vitality were improved in all patients after F technique was compared to U technique. With U technique, eight patients on eight had negative defrost test; after F technique, seven patients on eight had a positive defrost test and could therefore have access to assisted reproduction techniques. Four couples had five ICSI and obtained three pregnancies leading to five births.
  
  
  CONCLUSIONS
  Endovesical instillation of sterile spermatozoa culture medium before ejaculation was a safe and effective technique to improve spermatozoa quality in male infertility related to refractory retrograde ejaculation.
authors.affiliation: CECOS, Laboratoire de Biologie de la Reproduction, Assistance Publique-Hôpitaux de Marseille La Conception, 13005 Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Perrin
authors.forename: J
authors.initials: J
authors.lastname: Saïas-Magnan
authors.forename: A
authors.initials: A
authors.lastname: Lanteaume
authors.forename: I
authors.initials: I
authors.lastname: Thiry-Escudié
authors.forename: G
authors.initials: G
authors.lastname: Serment
authors.forename: F
authors.initials: F
authors.lastname: Bladou
authors.forename: M
authors.initials: M
authors.lastname: Gamerre
authors.forename: J-M
authors.initials: JM
authors.lastname: Grillo
authors.forename: G
authors.initials: G
authors.lastname: Karsenty
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004542:Ejaculation; D006801:Humans; D007248:Infertility, Male / Q000209:etiology*; D008297:Male; D011446:Prospective Studies; D012735:Sexual Dysfunction, Physiological / Q000150:complications*; D053625:Sperm Retrieval