title: Effects of prolonged warm and cold ischemia in a solitary kidney animal model after partial nephrectomy: an ultrastructural investigation.
abstract: Ischemia-reperfusion injury can be detrimental to the solitary kidney, especially when it is accompanied by ablation. To the authors' knowledge, the effects of partial nephrectomy with prolonged application of ischemia have never been described at the ultrastructural level. Therefore, the authors used an animal model and focused on putative structural effects in the glomerular basement membrane and the podocytes. They demonstrate the advantageous role of cold ischemia, even in up to 120 min. In contrast, more than 60 min of warm ischemia leads to catastrophic lesions in all the cellular structures, as is reflected by mortality due to acute renal failure.
authors.affiliation: Department of Urology, Athens University Medical School-LAIKO Hospital, Athens, Greece. statyr@freemail.gr
authors.forename: Stavros I
authors.initials: SI
authors.lastname: Tyritzis
authors.forename: Michael
authors.initials: M
authors.lastname: Zachariades
authors.forename: Kostas
authors.initials: K
authors.lastname: Evangelou
authors.forename: Vassilis G
authors.initials: VG
authors.lastname: Gorgoulis
authors.forename: Aspasia
authors.initials: A
authors.lastname: Kyroudi-Voulgari
authors.forename: Kitty
authors.initials: K
authors.lastname: Pavlakis
authors.forename: Theodoros G
authors.initials: TG
authors.lastname: Troupis
authors.forename: Constantinos A
authors.initials: CA
authors.lastname: Constantinides
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D000818:Animals; D050377:Cold Ischemia; D050533:Glomerular Basement Membrane / Q000648:ultrastructure; D007668:Kidney / Q000601:surgery / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D009392:Nephrectomy / Q000009:adverse effects; D050199:Podocytes / Q000648:ultrastructure; D011817:Rabbits; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D013997:Time Factors; D052096:Warm Ischemia / Q000009:adverse effects