title: Effect of genistein on p90RSK phosphorylation and cell proliferation in T47D breast cancer cells.
abstract: BACKGROUND
  The molecular mechanisms of genistein's proliferative effects on breast cancer cells are largely unknown. This study aimed to examine estrogen-receptor (ER)-related signaling molecules involved in genistein-associated cell proliferation and survival (ERK1/2, p90RSK, JNK, Akt and NFκB) and to correlate these results to cell proliferation.
  
  
  METHODS
  The effect of genistein on cell-signaling molecules was determined in T47D breast cancer cells by a Bioplex phosphoprotein detection kit. These results were confirmed by Western blotting and were correlated to cell proliferation by MTT assay.
  
  
  RESULTS
  Low and high concentrations of genistein induced an ERK1/2-independent decrease in phosphorylated p90RSK. This effect was accompanied by decreased cell proliferation at high concentrations and an increased response at low concentrations of genistein following a 48-hour exposure.
  
  
  CONCLUSIONS
  Concentration-dependent actions of genistein in T47D cells may be due to differential activation of signaling molecules.
authors.affiliation: Department of Biological Sciences, DePaul University, Chicago, IL 60614, USA.
authors.forename: John
authors.initials: J
authors.lastname: Gwin
authors.forename: Neil
authors.initials: N
authors.lastname: Drews
authors.forename: Syed
authors.initials: S
authors.lastname: Ali
authors.forename: Justin
authors.initials: J
authors.lastname: Stamschror
authors.forename: Matthew
authors.initials: M
authors.lastname: Sorenson
authors.forename: Talitha T
authors.initials: TT
authors.lastname: Rajah
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D011960:Receptors, Estrogen / Q000378:metabolism; D038744:Ribosomal Protein S6 Kinases, 90-kDa / Q000378:metabolism*
chemical_list: D016588:Anticarcinogenic Agents; D011960:Receptors, Estrogen; D019833:Genistein; D038744:Ribosomal Protein S6 Kinases, 90-kDa