title: Improved temporal resolution and linked hidden Markov modeling for switchable single-molecule FRET.
abstract: Switchable FRET is the combination of single-molecule Förster resonance energy transfer (smFRET) with photoswitching, the reversible activation and deactivation of fluorophores by light. By photoswitching, multiple donor-acceptor fluorophore pairs can be probed sequentially, thus allowing observation of multiple distances within a single immobilized molecule. Control of the photoinduced switching rates permits adjustment of the temporal resolution of switchable FRET over a wide range of timescales, thereby facilitating application to various dynamical biological systems. We show that fast total internal reflection (TIRF) microscopy can achieve measurements of two FRET pairs with 10 ms temporal resolution within less than 2 s. The concept of switchable FRET is also compatible with confocal microscopy on immobilized molecules, providing better data quality at high temporal resolution. To identify states and extract their transitions from switchable FRET time traces, we also develop linked hidden Markov modeling (HMM) of both FRET and donor-acceptor stoichiometry. Linked HMM successfully identifies transient states in the two-dimensional FRET-stoichiometry space and reconstructs their connectivity network. Improved temporal resolution and novel data analysis make switchable FRET a valuable tool in molecular and structural biology.
authors.affiliation: Department of Physics and Biological Physics Research Group, University of Oxford, Parks Road, Oxford OX1 3PU, UK.
authors.forename: Stephan
authors.initials: S
authors.lastname: Uphoff
authors.forename: Kristofer
authors.initials: K
authors.lastname: Gryte
authors.forename: Geraint
authors.initials: G
authors.lastname: Evans
authors.forename: Achillefs N
authors.initials: AN
authors.lastname: Kapanidis
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D008390:Markov Chains; D008856:Microscopy, Fluorescence; D009010:Monte Carlo Method
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C085321:cyanine dye 5