title: Stabilizing effect of polyols is sensitive to inherent stability of protein.
abstract: In studies on polyol-mediated protein stabilization, the polyols are the preferred variable and less importance is given to the intrinsic properties of the protein used. We investigated the stabilizing effects of glycerol on three in vitro evolved lipase mutants with varying stabilities and also in a broad pH range of 3.3-12.1. Significant linear negative correlation between increment in stability due to glycerol and prior stability suggests that stabilizing effects of glycerol depend on the prior stability of the protein. Polar/nonpolar surface area and charge do not have a bearing on the stabilizing effects of glycerol.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India. zahid@ccmb.res.in
authors.forename: Md Zahid
authors.initials: MZ
authors.lastname: Kamal
authors.forename: Shoeb
authors.initials: S
authors.lastname: Ahmad
authors.forename: Nalam Madhusudhana
authors.initials: NM
authors.lastname: Rao
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D005990:Glycerol / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D011489:Protein Denaturation; D055550:Protein Stability
chemical_list: D008049:Lipase; D005990:Glycerol