title: Combined analysis by GC (RI), GC-MS and 13C NMR of the supercritical fluid extract of Abies alba twigs.
abstract: Two samples (leaves and twigs) of Abies alba Miller from Corsica were extracted using supercritical CO2 and their chemical compositions were compared with those of the essential oils obtained from the same batch of plant material. In total 45 components were identified using combined analysis by GC (RI), GC-MS and 13C NMR. It was observed that the contents of monoterpenes (mainly represented by limonene, alpha-pinene and camphene) were significantly lower in the supercritical fluid extract (SFE) than in the essential oil (EO). Conversely, the proportions of sesquiterpenes were much higher in CO2 extracts than in essential oils (around 30% vs 4%). Cis-abienol, a diterpene alcohol, was identified only in SFE, and the proportions of this constituent (7.5% and 17.3%) were determined using quantitative 13C NMR since it was under estimated using the standard conditions of GC.
authors.affiliation: Université de Corse-CNRS, UMR 6134 SPE, Laboratoire "Chimie et Biomasse ", Route des Sanguinaires, 20 000 Ajaccio, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Duquesnoy
authors.forename: Bruno
authors.initials: B
authors.lastname: Marongiu
authors.forename: Vincent
authors.initials: V
authors.lastname: Castola
authors.forename: Alessandra
authors.initials: A
authors.lastname: Piras
authors.forename: Silvia
authors.initials: S
authors.lastname: Porcedda
authors.forename: Joseph
authors.initials: J
authors.lastname: Casanova
mesh_terms: D028202:Abies / Q000737:chemistry*; D002849:Chromatography, Gas; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D009822:Oils, Volatile / Q000032:analysis*
chemical_list: D009822:Oils, Volatile