title: Decreased renal mRNA expression of TRPM6 is associated with hypomagnesemia in C57BL/6 asthmatic mice.
abstract: OBJECTIVE
  Hypomagnesemia has been reported up to 40% in asthma patients, and a relationship between hypomagnesemia and asthma severity has been previously characterized. However, the mechanism for hypomagnesemia in asthma patients is not clear. Transient receptor potential melastatin 6 (TRPM6) is a newly identified channel that is involved in active epithelial magnesium transport, and downregulation of TRPM6 in the kidney was related to reduced Mg2+ reabsorption in mouse model. The aim of the study was to investigate whether plasma and erythrocyte magnesium levels were correlated with renal expression of TRPM6 mRNA in C57BL/6 asthmatic mice.
  
  
  METHODS
  48 healthy C57BL/6 mice were randomly divided into asthmatic group and control group with 24 mice in each group. Each group were randomly taken out 8 mice at 1d, 21d, 34d to detect plasma Mg2+, intracellular Mg2+ and renal TRPM6 mRNA expressions.
  
  
  RESULTS
  There were no significant difference in plasma Mg2+, intracellular Mg2+ and TRPM6 mRNA expression of renal tissues between asthmatic group and control group at 1d. However, plasma Mg2+ and intracellular Mg2+ as well as TRPM6 mRNA of renal tissues in asthmatic group were significantly lower than that of control group at 21d and at 34d. Both plasma Mg2+ and intracellular Mg2+ were positively correlated with TRPM6 mRNA expression in the renal tissues.
  
  
  CONCLUSIONS
  This study indicates that the consistently reduced expression of TRPM6 mRNA may play a role in the pathogenesis of hypomagnesemia in C57BL/6 asthmatic mice.
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, PR China. shanpingjiang@126.com
authors.forename: S P
authors.initials: SP
authors.lastname: Jiang
authors.forename: Y M
authors.initials: YM
authors.lastname: Wu
authors.forename: S E
authors.initials: SE
authors.lastname: Guo
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Lv
mesh_terms: D000818:Animals; D001249:Asthma / Q000235:genetics / Q000378:metabolism*; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism*; D008274:Magnesium / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050053:TRPM Cation Channels / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D050053:TRPM Cation Channels; C530168:Trpm6 protein, mouse; D008274:Magnesium