title: Use of evolutionary operation (EVOP) factorial design technique to develop a bioprocess using grease waste as a substrate for lipase production.
abstract: The aim of the present work was to develop a bioprocess using EVOP-factorial design technique employing grease waste as a substrate for the production of lipase. A newly isolated fungal strain of Penicillium chrysogenum was explored for the fermentation process. Solid-state fermentation (SSF) was carried out using grease waste and Czapek-dox medium, supplemented with wheat bran. The yield of lipase was 38 U/ml when SSF was carried out at 32 °C for 8 days and grease:wheat bran:Czapek-dox media in 1:1:2 (w/w/v). Different physicochemical parameters affecting the production of lipase were optimized through evolutionary operation (EVOP) factorial design technique and after optimization yield was enhanced up to 46 U/ml at 30 °C, pH 7.0 with 1:1:2 (w/w/v) grease waste:wheat bran:Czapek-dox media. Industrial grease waste has never been reported before for the production of industrially important lipase enzyme.
authors.affiliation: Biotechnology Section, Applied Mechanics Department, Motilal Nehru National Institute of Technology, Allahabad 211 004, Uttar Pradesh, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Nitin
authors.initials: N
authors.lastname: Katiyar
authors.forename: Priyanka
authors.initials: P
authors.lastname: Ingle
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Negi
mesh_terms: D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D008049:Lipase / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000201:enzymology*; D010578:Petroleum / Q000378:metabolism*; D014866:Waste Products
chemical_list: D010578:Petroleum; D014866:Waste Products; D008049:Lipase