title: P2 porin and loop L7 from Haemophilus influenzae modulate expression of IL-6 and adhesion molecules in astrocytes.
abstract: During neuropathological conditions such as infections and degenerative diseases, astrocytes can be activated by infiltrating immune cells. Activated astrocytes can produce chemokines, cytokines and adhesion molecules. In this study, the production of IL-6 and adhesion molecules intercellular adhesion molecule-1 (ICAM-1), vascular cellular adhesion molecule-1 (VCAM-1) and E-selectin by human astroglioma cells stimulated with Gram-negative surface components was investigated. Haemophilus influenzae type b porin P2 and its selected active peptide, loop L7, were found to induce MEK1-MEK2/ mitogen-activated protein kinase phosphorylation in U87-MG cells as demonstrated by ELISA, and up-regulate cellular adhesion molecule and interleukin-6 (IL-6) production as shown by RT-PCR and ELISA. Using two potent and selective inhibitors of MEK activation by Raf-1 (PD-098059) and p38 (SB-203580), it was also demonstrated that both ERK1/2 and p38 pathways play key roles in the production of IL-6 as well as in ICAM-1, VCAM-1 and E-selectin expression by Hib porin.
authors.affiliation: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, Second University of Naples, Naples, Italy.
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Vitiello
authors.forename: Emiliana
authors.initials: E
authors.lastname: Finamore
authors.forename: Marco
authors.initials: M
authors.lastname: Cantisani
authors.forename: Paolo
authors.initials: P
authors.lastname: Bevilacqua
authors.forename: Novella
authors.initials: N
authors.lastname: Incoronato
authors.forename: Annarita
authors.initials: A
authors.lastname: Falanga
authors.forename: Emilia
authors.initials: E
authors.lastname: Galdiero
authors.forename: Marilena
authors.initials: M
authors.lastname: Galdiero
mesh_terms: D001253:Astrocytes / Q000276:immunology / Q000378:metabolism*; D001426:Bacterial Proteins / Q000276:immunology* / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism; D006193:Haemophilus influenzae / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D018272:Porins / Q000276:immunology* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D015850:Interleukin-6; D018272:Porins; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C451502:ompP2 protein, Haemophilus influenzae; D018799:Intercellular Adhesion Molecule-1; D020928:Mitogen-Activated Protein Kinases