title: A model of the C14-EPIC complex indicates hotspots for a protease-inhibitor arms race in the oomycete-potato interaction.
abstract: The oomycete pathogen Phytophthora infestans secretes cystatin-like effector proteins (EPICs) that inhibit secreted host proteases during infection. We recently found that the C14 protease is a relevant target of EPICs and that this protease is under diversifying selection in wild potato species with which P. infestans has coevolved. Here we generated a model of the EPIC-C14 complex based on cystatin-papain crystal structures and discovered three regions where variant residues in C14 might be the result of an arms race between enzyme and inhibitor at the plant-pathogen interface.
authors.affiliation: Plant Chemetics Laboratory, Chemical Genomics Centre of the Max Planck Society, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
authors.forename: Farnusch
authors.initials: F
authors.lastname: Kaschani
authors.forename: Renier A L
authors.initials: RA
authors.lastname: Van der Hoorn
mesh_terms: D000595:Amino Acid Sequence; D054884:Host-Pathogen Interactions; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D055750:Phytophthora infestans; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011480:Protease Inhibitors / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011198:Solanum tuberosum / Q000201:enzymology* / Q000382:microbiology*; D013379:Substrate Specificity
chemical_list: D010940:Plant Proteins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases