title: Administration of adenovirus encoding anti-CD20 antibody gene induces B-cell deletion and alleviates lupus in the BWF1 mouse model.
abstract: Increasing evidence demonstrates that pathological B cells play an essential role in the triggering and development of human systemic lupus erythematosus (SLE). A rational strategy for treating SLE might be to delete B cells thereby suppressing autoimmunity. Commercial monoclonal anti-CD20 antibody is widely used for treatment of B cell-related autoimmune disorders. However its long term use is limited by several factors including short half-life, high cost, and possible side effects of antibody protein therapy. Therefore, we constructed a recombinant adenovirus encoding the murine anti-CD20 antibody gene, and used it to immunize lupus-prone (BWF1) mice. Our data demonstrated that administration of adenovirus encoding the murine anti-CD20 antibody gene generated murine anti-CD20 antibody, which resulted in elimination of B cells in BWF1 mice. In addition, the anti-CD20 reduced serum anti-dsDNA antibody levels, impeded the development of proteinuria and improved the survival of BWF1 mice. These findings suggested that the adenovirus encoding murine anti-CD20 antibody gene might provide an alternative strategy for B cell-mediated diseases.
authors.affiliation: Center Laboratory Department, The General Hospital of Chengdu Army, Chengdu, China. wcs300k@163.com
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000257:Adenoviridae Infections / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000378:metabolism; D018951:Antigens, CD20 / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005822:Genetic Vectors; D006801:Humans; D007167:Immunotherapy; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000628:therapy; D008212:Lymphocyte Depletion; D051379:Mice; D008817:Mice, Mutant Strains; D011507:Proteinuria / Q000517:prevention & control; D019076:Transgenes / Q000235:genetics
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D018951:Antigens, CD20