title: Nested graft in chronic wounds: a new solution for an old problem.
abstract: It is well shown that chronic wounds are populated by cells unable to respond to re-epithelising stimulus. Large ulcers that remain unhealed for several months are more difficult to treat probably because of the depletion of active factors. Yet in 1869 Reverdin realised that the partial coverage of an ulcer with small fragments of healthy skin was able to lead to wound healing; unfortunately, its employment was limited to granulating wounds. Recently, the importance of factors such as cytokines, chemokines and adhesion molecules in wound healing, and the involvement of all cellular types resident or transiting in the skin has been partially elucidated. In this study, we proposed to simultaneously provide a new cellular and molecular reservoir with the efficient stimulus to trigger it. We created receiving site inside the ulcer, able to contain a full-thickness graft taken from a donor site. Our aim was not to cover the entire defect, but to use the minigraft as 'fount' of functional cells and to give an acute stress through the chambers created inside the ulcer. A complete wound healing was obtained in all patients treated in a short period of time. This technique does not require special equipment and assistance in maintaining costs at very low levels.
authors.affiliation: Department of Dermatology, A.O. Spedali Civili, Brescia, Italy. giuliogualdi@libero.it
authors.forename: Giulio
authors.initials: G
authors.lastname: Gualdi
authors.forename: Paola
authors.initials: P
authors.lastname: Monari
authors.forename: Camillo
authors.initials: C
authors.lastname: Farisoglio
authors.forename: Piergiacomo
authors.initials: P
authors.lastname: Calzavara-Pinton
mesh_terms: D000368:Aged; D002908:Chronic Disease; D003646:Debridement / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D016038:Skin Transplantation / Q000379:methods*; D016896:Treatment Outcome; D014647:Varicose Ulcer / Q000473:pathology / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*