title: Single particle tracking as a method to resolve differences in highly colocalized proteins.
abstract: Single particle tracking fluorescence microscopy was used to study two late endosomal proteins, Rab7 and LAMP1, that appear to be highly colocalized in static fluorescence microscopy images. Imaging these proteins simultaneously reveals that Rab7 and LAMP1 undergo periods of separation within the cell. Single particle tracking carried out during these periods of separation shows that Rab7-vesicles have greater velocities, but undergo less efficient transport than LAMP1-vesicles. This research demonstrates the use of single particle tracking as a tool to resolve functional differences in highly colocalized proteins in intact live cells.
authors.affiliation: School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA.
authors.forename: Craig J
authors.initials: CJ
authors.lastname: Szymanski
authors.forename: William H
authors.initials: WH
authors.lastname: Humphries
authors.forename: Christine K
authors.initials: CK
authors.lastname: Payne
mesh_terms: D011992:Endosomes / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D052118:Lysosomal-Associated Membrane Protein 1 / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods*; D020691:rab GTP-Binding Proteins / Q000032:analysis*; D000090802:rab7 GTP-Binding Proteins
chemical_list: D052118:Lysosomal-Associated Membrane Protein 1; D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D020691:rab GTP-Binding Proteins