title: Identification of CD133+/nestin+ putative cancer stem cells in non-small cell lung cancer.
abstract: OBJECTIVE
  No effective treatment for lung cancer exists currently. One reason for this, is the development of drug resistance, assumed to be associated with cancer stem cell (CSCs) emergence within the tumour. This pilot study aimed to identify CSCs in 121 non-small cell lung cancer (NSCLC) patient samples via detection of the expression of stem cell markers - CD133 and nestin.
  
  
  METHODS
  Archived paraffin blocks of 121 patient samples were prepared as Tissue Microarrays (TMA). Indirect immunohistochemical staining was used to determine the level of expression of CD133 and nestin. Double immunofluorescence staining was used to investigate the co-expression of these two markers. To determine the correlation between expression of nestin and CD133 with the length of asymptomatic period and overall patient survival we used the Kaplan-Meyer analysis.
  
  
  RESULTS
  CD133 expression was detected in 22 (19%), nestin in the epithelium in 74 (66%) and vasculature in 78 (70%) of patients. Co-expression of these two markers was found in 21 (17%) patients in less than 1% of positive cells without impact on disease free or overall survival.
  
  
  CONCLUSIONS
  We identified CD133(+)/nestin(+) cells as novel potential markers of lung cancer CSCs.
authors.affiliation: Department of Pathology and Laboratory of Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc, University Hospital Olomouc, Czech Republic. maja.jan@centrum.cz
authors.forename: Maria
authors.initials: M
authors.lastname: Janikova
authors.forename: Jozef
authors.initials: J
authors.lastname: Skarda
authors.forename: Marta
authors.initials: M
authors.lastname: Dziechciarkova
authors.forename: Lenka
authors.initials: L
authors.lastname: Radova
authors.forename: Jana
authors.initials: J
authors.lastname: Chmelova
authors.forename: Veronika
authors.initials: V
authors.lastname: Krejci
authors.forename: Eva
authors.initials: E
authors.lastname: Sedlakova
authors.forename: Jana
authors.initials: J
authors.lastname: Zapletalova
authors.forename: Katerina
authors.initials: K
authors.lastname: Langova
authors.forename: Jiri
authors.initials: J
authors.lastname: Klein
authors.forename: Ivona
authors.initials: I
authors.lastname: Grygarkova
authors.forename: Vitezslav
authors.initials: V
authors.lastname: Kolek
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D001808:Blood Vessels / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008175:Lung Neoplasms / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D010455:Peptides / Q000378:metabolism*; D010865:Pilot Projects
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; C000606023:PROM1 protein, human; D010455:Peptides