title: Identification of peroxiredoxin-1 as a novel biomarker of abdominal aortic aneurysm.
abstract: OBJECTIVE
  In the search of novel biomarkers of abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed by a differential proteomic approach.
  
  
  RESULTS
  Different layers (luminal/abluminal) of the ILT of AAA were incubated, and the proteins released were analyzed by 2-dimensional difference in-gel electrophoresis. Several differentially expressed proteins involved in main AAA pathological mechanisms (proteolysis, oxidative stress, and thrombosis) were identified by mass spectrometry. Among the proteins identified, peroxiredoxin-1 (PRX-1) was more released by the luminal layer compared with the abluminal layer of the ILT, which was further validated by Western blot, ELISA, and immunohistochemistry. We demonstrated increased PRX-1 serum levels in AAA patients compared with healthy subjects and also positive correlation among PRX-1 and AAA diameter, plasmin-antiplasmin, and myeloperoxidase levels. Finally, a prospective study revealed a positive correlation between PRX-1 serum levels and AAA expansion rate. Moreover, the combination of PRX-1 and AAA size had significantly additive value in predicting growth.
  
  
  CONCLUSIONS
  Several proteins associated with AAA pathogenesis have been identified by a proteomic approach in ILT-conditioned medium. Among them, PRX-1 serum levels are increased in AAA patients and correlate with AAA size and growth rate, suggesting the potential use of PRX-1 as a biomarker for AAA evolution.
authors.affiliation: ascular Research Laboratory, Instituto de Investigación Sanitaria-Fundación Jiménez Diaz-Autonoma University, Madrid, Spain.
authors.forename: Roxana
authors.initials: R
authors.lastname: Martinez-Pinna
authors.forename: Priscila
authors.initials: P
authors.lastname: Ramos-Mozo
authors.forename: Julio
authors.initials: J
authors.lastname: Madrigal-Matute
authors.forename: Luis M
authors.initials: LM
authors.lastname: Blanco-Colio
authors.forename: Juan A
authors.initials: JA
authors.lastname: Lopez
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Emilio
authors.initials: E
authors.lastname: Camafeita
authors.forename: Jes S
authors.initials: JS
authors.lastname: Lindholt
authors.forename: Olivier
authors.initials: O
authors.lastname: Meilhac
authors.forename: Sandrine
authors.initials: S
authors.lastname: Delbosc
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Melina
authors.initials: M
authors.lastname: Vega de Ceniga
authors.forename: Jesus
authors.initials: J
authors.lastname: Egido
authors.forename: Jose L
authors.initials: JL
authors.lastname: Martin-Ventura
mesh_terms: D000368:Aged; D001012:Aorta, Abdominal / Q000000981:diagnostic imaging / Q000378:metabolism*; D017544:Aortic Aneurysm, Abdominal / Q000000981:diagnostic imaging / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D003718:Denmark; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000097:blood / Q000378:metabolism*; D011446:Prospective Studies; D040901:Proteomics / Q000379:methods; D012372:ROC Curve; D013030:Spain; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014463:Ultrasonography; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C515940:PRDX1 protein, human; D054464:Peroxiredoxins