title: Effect of asymmetric dimethylarginine on MIF expression and TNF-α and IL-8 secretion in THP-1 monocytes-derived macrophages.
abstract: OBJECTIVE
  To investigate the effect of ADMA on macrophage migration inhibitory factor (MIF) expression and tumor necrosis factor-α (TNF-α) and IL-8 secretion in THP-1 monocyte-derived macrophages. METHIDS: THP-1 monocytes were induced to differentiate into macrophages by a 24-h incubation with 160 nmol/L PMA. The THP-1 monocyte-derived macrophages were exposed to different concentrations of ADMA for 24 h, and the changes in MIF mRNA and protein expressions were analyzed with RT-PCR and Western blotting, respectively. Enzyme-linked immunosorbent assay was used to detect the levels of TNF-α and IL-8 in the supernatant of THP-1-derived macrophages following ADMA treatments.
  
  
  RESULTS
  ADMA obviously up-regulated MIF mRNA and protein expressions in THP-1-derived macrophages in a concentration- dependent manner. Exposure of the cells to 15 µmol/L ADMA for 24 h showed the most potent effect in up-regulating MIF mRNA and protein expressions. ADMA treatment also resulted in a dose-dependent increase of the levels of TNF-α and IL-8 in the culture supernatant of the macrophages, and the peak levels occurred following the treatment with 15 µmol/L ADMA.
  
  
  CONCLUSIONS
  ADMA can up-regulate MIF expression and induce TNF-α and IL-8 secretion in THP-1 monocyte-derived macrophages.
authors.affiliation: Kunming Medical University, Kunming 650031, China. zhuzhendong77@sina.com.cn
authors.forename: Zhen-dong
authors.initials: ZD
authors.lastname: Zhu
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Yu
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yong-jin
authors.initials: YJ
authors.lastname: Wang
authors.forename: Dian-hua
authors.initials: DH
authors.lastname: Wang
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology; D010616:Phenanthrenes / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C410511:1-keto-1,2,3,4-tetrahydrophenanthrene; D016209:Interleukin-8; D008263:Macrophage Migration-Inhibitory Factors; D010616:Phenanthrenes; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C018524:N,N-dimethylarginine; D001120:Arginine; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human