title: Condylar cartilaginous changes after mandibular distraction osteogenesis in rabbits.
abstract: OBJECTIVE
  The purpose of this study was to evaluate histologic and immunohistochemical changes in the condylar articular surface in response to distraction osteogenesis (DO) of the mandibular body in rabbits.
  
  
  METHODS
  A unilateral osteotomy of the mandible at the premolar area was performed in 16 rabbits. The animals were divided into 4 groups based on different distraction parameters (rate and rhythm). After a 5-day latency, the mandible was lengthened by 0.5 mm daily for 6 days (group 1); 0.5 mm twice daily for 3 days (group 2); 0.5 mm once daily for 10 days (group 3); or 0.5 mm twice daily for 5 days (group 4). All 4 groups underwent a 14-day consolidation period. Four rabbits were included in the control group. The specimens were stained with hematoxylin-eosin for histologic examination. Immunohistochemical analysis was performed to evaluate the expression of growth factors.
  
  
  RESULTS
  None of the groups demonstrated any degenerative changes in the temporomandibular joint (TMJ). On the distraction side in all groups, the histopathological examination revealed a hypertrophic thickening of the cartilage zone. Prominent endochondral ossification and high active osteoblasts were observed in groups 3 and 4. On the nondistraction side, no major changes were observed excluding the appearance of osteoclasts in groups 3 and 4. The immunohistochemical analysis revealed tenascin immunoreactivity in bone marrow mesenchymal cells on the distraction side in group 4. Connexin immunoreactivity did not display a marked change in any of the groups. Osteocalcin was observed on the distraction side in group 2, which suggested that bone formation is increased. Nitric oxide synthase 2 immunoreactivity was observed on the distraction side in group 2, which is associated with stress and inflammation.
  
  
  CONCLUSIONS
  The results indicated that the hypertrophy of the cartilage zone and endochondral ossification became more pronounced as the extent and rate of distraction increased.
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Chosun University, DongGu, Gwangju, Republic of Korea.
authors.forename: Sin-Young
authors.initials: SY
authors.lastname: Ahn
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology*; D019902:Chondrocytes / Q000473:pathology; D003094:Collagen; D017630:Connexins / Q000032:analysis; D006984:Hypertrophy; D007150:Immunohistochemistry; D008334:Mandible / Q000473:pathology / Q000601:surgery*; D008335:Mandibular Condyle / Q000473:pathology*; D008648:Mesoderm / Q000473:pathology; D052247:Nitric Oxide Synthase Type II / Q000032:analysis; D010006:Osteoblasts / Q000473:pathology; D015675:Osteocalcin / Q000032:analysis; D010010:Osteoclasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D019857:Osteogenesis, Distraction / Q000295:instrumentation / Q000379:methods*; D010027:Osteotomy / Q000379:methods; D011817:Rabbits; D019063:Tenascin / Q000032:analysis; D013997:Time Factors
chemical_list: D017630:Connexins; D019063:Tenascin; D015675:Osteocalcin; D003094:Collagen; D052247:Nitric Oxide Synthase Type II