title: Crystallization and preliminary X-ray analysis of the human long myosin light-chain kinase 1-specific domain IgCAM3.
abstract: Myosin light-chain kinase-dependent tight junction regulation is a critical event in inflammatory cytokine-induced increases in epithelial paracellular permeability. MLCK is expressed in human intestinal epithelium as two isoforms, long MLCK1 and long MLCK2, and MLCK1 is specifically localized to the tight junction, where it regulates paracellular permeability. The sole difference between these long MLCK splice variants is the presence of an immunoglobulin-like cell-adhesion molecule domain, IgCAM3, in MLCK1. To gain insight into the structure of the IgCAM3 domain, the IgCAM3 domain of MLCK1 has been expressed, purified and crystallized. Preliminary X-ray diffraction data were collected to 2.0 Å resolution and were consistent with the primitive trigonal space group P2(1)2(1)2(1).
authors.affiliation: Department of Pathology, University of Chicago, Chicago, IL 60637, USA.
authors.forename: W Vallen
authors.initials: WV
authors.lastname: Graham
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Magis
authors.forename: Kate M
authors.initials: KM
authors.lastname: Bailey
authors.forename: Jerrold R
authors.initials: JR
authors.lastname: Turner
authors.forename: David A
authors.initials: DA
authors.lastname: Ostrov
mesh_terms: D000595:Amino Acid Sequence; D005591:Chemical Fractionation; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism / Q000502:physiology; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049449:Luminescence; D008969:Molecular Sequence Data; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D019108:Tight Junctions / Q000378:metabolism / Q000502:physiology; D014961:X-Ray Diffraction; D014965:X-Rays
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D009219:Myosin-Light-Chain Kinase