title: [Laser Raman and infrared spectrum analysis of low-density lipoproteins purified from hen egg yolk].
abstract: During the experiment, diversified proteins were separated from hen egg yolk by ammonium sulphate rapid fractionation, and pure LDL was obtained after filtrating through Sephadex G-200 chromatography. After the qualitative detection of SDS-PAGE, the authors discovered that LDL consists of five major apoprotein. The Raman and infrared spectrum showed CH2 asymmetric stretching and symmetric stretching mode. However, the authors found C==O stretching vibrations of protein peptide bonds and N+ (CH3)3 asymmetric stretching vibration from the choline group in phospholipids. Laser Raman and infrared spectrum analysis of LDL provided useful information for studying their structure.
authors.affiliation: Laboratory of Biophysics and Biomedicine, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China. mr.dream.love@163.com
authors.forename: Hui-jun
authors.initials: HJ
authors.lastname: Xue
authors.forename: Run-guang
authors.initials: RG
authors.lastname: Sun
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Yi-guang
authors.initials: YG
authors.lastname: Chang
mesh_terms: D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007834:Lasers; D008077:Lipoproteins, LDL / Q000032:analysis*; D010743:Phospholipids; D013055:Spectrophotometry, Infrared; D013059:Spectrum Analysis, Raman; D014732:Vibration
chemical_list: D008077:Lipoproteins, LDL; D010743:Phospholipids