title: Channel catfish CD8α and CD8β co-receptors: characterization, expression and polymorphism.
abstract: In this study we report the identification and characterization of channel catfish, Ictalurus punctatus CD8α and CD8β genes. Both genes encode predicted proteins containing a leader, a immunoglobulin superfamily V domain, a stalk/hinge region, a transmembrane region and a positively charged cytoplasmic tail (CYT) containing the conserved teleost C-X-H motif. Catfish CD8α and CD8β are encoded as single copy genes and as in other vertebrates exhibit a conserved head to tail synteny; the CD8β gene is found 14.1kb upstream of the CD8α gene. Both CD8α and CD8β transcripts showed a low degree of polymorphism. Finally, as determined by q-PCR both CD8α and CD8β are expressed in various catfish lymphoid tissues with the highest expression observed in thymus from 2 month old catfish-fry. In the future these results will provide the basis for evaluating the role of CD8(+) CTL and other CD8-bearing cells in response to immunization or infection in the catfish.
authors.affiliation: Sylvie.Quiniou@ARS.USDA.GOV
authors.forename: Sylvie M A
authors.initials: SM
authors.lastname: Quiniou
authors.forename: Manoranjan
authors.initials: M
authors.lastname: Sahoo
authors.forename: Eva-Stina
authors.initials: ES
authors.lastname: Edholm
authors.forename: Eva
authors.initials: E
authors.lastname: Bengten
authors.forename: Melanie
authors.initials: M
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016827:CD8 Antigens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007059:Ictaluridae / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain