title: Increased numbers of tumor-lysing monocytes in cancer patients.
abstract: Lymphatic infiltration is a well known phenomenon in different tumors including endocrine malignancies. However, little is known about the role of antigen-presenting cells and T cell activation in this context. The aim of our study was to investigate the quantity and function of CD14+/CD56+ monocytes in tumor patients including endocrine malignancies. First, these cells were characterized in peripheral blood of endocrine and non-endocrine cancer patients as well as in tumor tissue samples. Cancer patients had in mean 3.7 times more CD14+/CD56+ monocytes in the peripheral blood compared to healthy controls (p≤0.0001), while the highest frequencies were seen in patients with heavy tumor load. Importantly, these cells additionally expressed several NK cell markers. A proof of CD14+/CD56+ infiltrations into papillary thyroid carcinoma was shown by immunohistochemical analyses. Functional analyses revealed an apoptosis inducing capacity in vitro after IFN-α re-stimulation. Our data indicate the importance of tumor-lysing monocytes in antitumor immunity.
authors.affiliation: Endocrine Tumor Center, Department of Endocrinology, Diabetes and Rheumatology, University Hospital Duesseldorf, Germany. claudia.papewalis@uni-duesseldorf.de
authors.forename: Claudia
authors.initials: C
authors.lastname: Papewalis
authors.forename: Benedikt
authors.initials: B
authors.lastname: Jacobs
authors.forename: Anna M
authors.initials: AM
authors.lastname: Baran
authors.forename: Margret
authors.initials: M
authors.lastname: Ehlers
authors.forename: Nikolas H
authors.initials: NH
authors.lastname: Stoecklein
authors.forename: Holger S
authors.initials: HS
authors.lastname: Willenberg
authors.forename: Sven
authors.initials: S
authors.lastname: Schinner
authors.forename: Martin
authors.initials: M
authors.lastname: Anlauf
authors.forename: Andreas
authors.initials: A
authors.lastname: Raffel
authors.forename: Kenko
authors.initials: K
authors.lastname: Cupisti
authors.forename: Roland
authors.initials: R
authors.lastname: Fenk
authors.forename: Werner A
authors.initials: WA
authors.lastname: Scherbaum
authors.forename: Matthias
authors.initials: M
authors.lastname: Schott
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D019002:CD56 Antigen / Q000378:metabolism; D002277:Carcinoma; D002291:Carcinoma, Papillary; D016022:Case-Control Studies; D002452:Cell Count; D005260:Female; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008213:Lymphocyte Activation; D016246:Lymphocytes, Tumor-Infiltrating / Q000166:cytology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D017252:CD3 Complex; D019002:CD56 Antigen; D018950:Lipopolysaccharide Receptors; C522918:NCAM1 protein, human