title: Retrograde study of projections from the tuberomammillary nucleus to the mesopontine cholinergic complex in the rat.
abstract: The mesopontine cholinergic complex comprising the pedunculopontine (PPTg) and laterodorsal (LDTg) tegmental nuclei is a key component of ascending reticular activating system. The brainstem cholinergic nuclei are yet under the control of descending projections from hypothalamic arousal centers such as the hypocretinergic, lateral hypothalamus (LH), and the histaminergic, tuberomammillary nucleus (TMN). The present study was designed to determine the differential projection pattern as well as the laterality of descending TMN projections to the PPTg and LDTg. Our results showed that each TMN subdivision provided differential projections to the mesopontine complex. The majority of PPTg-projecting neurons were located in ventrolateral TMN mainly at its subpial border, whereas LDTg-projecting cells were in dorsomedial TMN with a few in the medial border of the ventrolateral subdivision. For both PPTg and LDTg cases, retrogradely labeled neurons were more pronounced in each TMN subdivision ipsilateral to the injection site. The light microscopic observation also indicated that hypocretinergic, terminal-like boutons formed close appositions to somata as well as proximal dendrites of PPTg- or LDTg-projecting TMN neurons. Taken together, the present observations suggested that each TMN subdivision provide differential projections to the mesopontine cholinergic complex and that the LH, via the TMN, provides indirect, descending projections to the complex as well.
authors.affiliation: Department of Anatomy, College of Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, 143-701 Seoul, South Korea.
authors.forename: Eun Y
authors.initials: EY
authors.lastname: Hong
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D005260:Female; D007026:Hypothalamic Area, Lateral / Q000166:cytology*; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D009434:Neural Pathways / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013681:Tegmentum Mesencephali / Q000166:cytology*
chemical_list: D000109:Acetylcholine