title: Protective effect of aqueous extract of Allium sativum against zearalenone toxicity mediated by oxidative stress.
abstract: Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. It has been implicated in several mycotoxicosis in farm animals and in humans. Several reports suggest that oxidative damage seems to be a key determinant of ZEN induced toxicity in vitro and in vivo. The aim of the current study was to evaluate the protective effects of aqueous extract of Allium sativum (AEA), against ZEN-induced cytotoxicity, reactive oxygen species (ROS) generation and DNA fragmentation in cultured Vero cells. Indeed, cytotoxicity effects were studied using MTT viability assay, ROS generation measurement and catalase activity induction. To check whether the oxidative stress induction was associated to DNA lesions, we looked for DNA fragmentation using Comet test. Our results indicated that ZEN induced several toxic effects and significant alterations mediated by oxidative stress mechanism. Treatment by ZEN combined to the lowest dose of AEA (250 μg/ml) showed a significant reduction of ZEN induced damages for all tested markers and a noticeable reduction of DNA fragmentation. It could be concluded that A. sativum aqueous extracts is effective in the protection against ZEN hazards. This could be relevant, particularly with the emergent demand for natural products which may counteract the detrimental toxic effects mediated by oxidative stress process and therefore prevents multiple human diseases.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Monastir University, Rue Avicenne, 5019 Monastir, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Abid-Essefi
authors.forename: C
authors.initials: C
authors.lastname: Zaied
authors.forename: C
authors.initials: C
authors.lastname: Bouaziz
authors.forename: I Ben
authors.initials: IB
authors.lastname: Salem
authors.forename: R
authors.initials: R
authors.lastname: Kaderi
authors.forename: H
authors.initials: H
authors.lastname: Bacha
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D020552:Comet Assay; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D005737:Garlic / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D014709:Vero Cells; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D004968:Estrogens, Non-Steroidal; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013481:Superoxides; D015025:Zearalenone; D002374:Catalase; D014969:Xanthine Oxidase