title: A novel artificial superoxide dismutase: non-covalent conjugation of albumin with a Mn(III) salophen type complex.
abstract: The approach of non-covalent conjugation has recently been used and a number of metal complexes have been encaged in a protein scaffold to form functional metallo proteins. Here we investigate a novel artificial superoxide dismutase enzyme based on the non-covalent conjugation of bovine serum albumin and the manganese(III) complex of N,N'-bis(salicylidene)-3,4-diaminobenzoic acid. We report the intriguing ability of the albumin environment to increase the SOD activity of the inorganic complex. We also determined the binding constant with BSA by CD spectroscopy.
authors.affiliation: Dipartimento di Scienze Chimiche, University of Catania, Vle ADoria 6, 95125 Catania, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Oliveri
authors.forename: Graziella
authors.initials: G
authors.lastname: Vecchio
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D008345:Manganese / Q000737:chemistry*; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013057:Spectrum Analysis; D013482:Superoxide Dismutase / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009942:Organometallic Compounds; D012459:Salicylates; C090416:salophen; D012710:Serum Albumin, Bovine; D008345:Manganese; D013482:Superoxide Dismutase