title: Expression pattern of clock under acute phase-delay of the light/dark cycle in the chicken pineal model.
abstract: Shift workers have a higher risk of metabolic syndrome, a condition that also develops in mice carrying mutation in their circadian clock gene clock. To collect more data on the transcriptional changes of clock under phase-shifted light/dark LD conditions, we examined the 24h patterns of clock mRNA expression in vivo and in vitro in chickens exposed acutely to a reversed LD (DL) cycle. Under controlled LD conditions (lights on at 6:00, lights off at 20:00), clock mRNA expression peaked in vivo at 2:00 (Zeitgeber Time 20, ZT20) and in vitro at 22:00 (ZT16). Even higher mRNA contents were measured in the first cycle of in vivo DL conditions between 22:00 and 6:00 (lights at night), but in the second cycle by 2:00, lower mRNA contents were detected than the control peak values seen at this time point. Furthermore, no alterations were found in vitro in clock mRNA content during the first 12h of DL conditions (lights at night). The differences seen between the first and the second DL cycles in vivo and between the in vivo and in vitro data for the first DL cycle support the idea that neurohumoral signals perturbed by a phase-delayed light-dark cycle may also play a role in the in vivo rapid transcriptional resetting of the circadian clock in the chicken pineal model.
authors.affiliation: Department of Anatomy, Medical School, University of Pécs, Hungary.
authors.forename: Siri
authors.initials: S
authors.lastname: Kommedal
authors.forename: Gergely
authors.initials: G
authors.lastname: Bódis
authors.forename: Attila
authors.initials: A
authors.lastname: Matkovits
authors.forename: Valér
authors.initials: V
authors.lastname: Csernus
authors.forename: András D
authors.initials: AD
authors.lastname: Nagy
mesh_terms: D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008027:Light; D023421:Models, Animal; D017440:Photoperiod; D010870:Pineal Gland / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors
chemical_list: D056926:CLOCK Proteins