title: Phylogenetic analysis of the 56 kDa protein genes of Orientia tsutsugamushi in Southwest Area of Korea.
abstract: This study was conducted to determine which genotypes were present in southwestern Korea. Nested polymerase chain reaction (PCR) and DNA sequence analysis targeting the Orientia tsutsugamushi-specific 56-kDa protein gene was performed with samples of blood and eschar. Of the 69 PCR-positive samples, 61 clustered with the Boryong previously isolated in Korea. CUH 4-6 had sequence homology of 100% with Kato, and CUH 4-3 had homology of 99.8% with Kato and formed the Kato cluster. CUH 4-57, CUH 4-31, CUH 4-142, and CUH 4-324 formed a Kawasaki cluster. CUH 4-271 had sequence homology of 100% with Jecheon and formed a Karp cluster. CUH 4-117 had homology of 99.8% with Neimeng-65, and Gilliam cluster. The most common genotype of O. tsutsugamushi in the southwestern part of Korea is the Boryong genotype. We also identified O. tsutsugamushi of the Kato, Neimeng-65 and Kawasaki genotypes, which had not been identified before in Korea.
authors.affiliation: Division of Infectious Diseases, Departments of Internal Medicine, School of Medicine, Chosun University, Gwangju, Korea. moksha1001@hanmail.net
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Jang
authors.forename: Ganesh Prasad
authors.initials: GP
authors.lastname: Neupane
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D012285:Orientia tsutsugamushi / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D012612:Scrub Typhus / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; C064828:Sta56 antigen, Rickettsia tsutsugamushi