title: In vitro cytotoxic and antiviral activities of Ficus carica latex extracts.
abstract: The latex of fig fruit (Ficus carica) is used in traditional medicine for the treatment of skin infections such as warts and also diseases of possible viral origin. Five extracts (methanolic, hexanic, ethyl acetate, hexane-ethyl acetate (v/v) and chloroformic) of this species were investigated in vitro for their antiviral potential activity against herpes simplex type 1 (HSV-1), echovirus type 11 (ECV-11) and adenovirus (ADV). To evaluate the capacity of the extracts to inhibit the replication of viruses, the following assays were performed: adsorption and penetration, intracellular inhibition and virucidal activity. Observation of cytopathic effects was used to determine the antiviral action. The hexanic and hexane-ethyl acetate (v/v) extracts inhibited multiplication of viruses by tested techniques at concentrations of 78 µg mL(-1). These two extracts were possible candidates as herbal medicines for herpes virus, echovirus and adenovirus infectious diseases. All extracts had no cytotoxic effect on Vero cells at all tested concentrations.
authors.affiliation: Laboratoire de Génétique, Biodiversité et Valorisation des Bio Ressources, UR 03ES09, Institut Supérieur de Biotechnologie, 5000 Monastir, Tunisie.
authors.forename: Houda
authors.initials: H
authors.lastname: Lazreg Aref
authors.forename: Badii
authors.initials: B
authors.lastname: Gaaliche
authors.forename: Abdelwaheb
authors.initials: A
authors.lastname: Fekih
authors.forename: Massoud
authors.initials: M
authors.lastname: Mars
authors.forename: Mahjoub
authors.initials: M
authors.lastname: Aouni
authors.forename: Jean
authors.initials: J
authors.lastname: Pierre Chaumon
authors.forename: Khaled
authors.initials: K
authors.lastname: Said
mesh_terms: D000256:Adenoviridae / Q000187:drug effects; D000818:Animals; D000998:Antiviral Agents / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D029822:Enterovirus B, Human / Q000187:drug effects; D030681:Ficus / Q000737:chemistry*; D018259:Herpesvirus 1, Human / Q000187:drug effects; D007840:Latex / Q000737:chemistry*; D010936:Plant Extracts / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D007840:Latex; D010936:Plant Extracts