title: The analysis of CD45 isoforms expression on HBV-specific T cells after liver transplantation.
abstract: This study aimed to analyze the expression of CD45 on HBV-specific T cells in patients who underwent liver transplantation (LT). We detected the distribution of two CD45 isoforms (CD45RA, CD45RO) and CD27 on peripheral blood lymphocytes including two HBV-specific pentamer cells by flow cytometry. Meanwhile, the levels of tacrolimus, plasma IL-7, and IL-15 were tested by ELISA. CD45RA decreased sharply after LT, whereas the expression of CD45RO(+) changed a very little. The proportion of CD4(+) CD27(+) subsets increased in the span from first 6-36 months after LT. With the up-regulation of IL-7, CD45RA(+) CD27(+) was reduced and CD45RO(+) was increased on HBV-specific T cells in LT group compared with CHB. Therefore, the switch from CD45RA to CD45RO probably depended on IL-7, without being stimulated by hepatitis B virus (HBV) and proliferated by IL-15. IL-7 could aid in maintaining HBV-specific T cells effectively during 3 years.
authors.affiliation: Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79# Qingchun Road, Hangzhou, Zhejiang Province 310003, China.
authors.forename: Lelin
authors.initials: L
authors.lastname: Pan
authors.forename: Wenjin
authors.initials: W
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000628:therapy / Q000821:virology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006509:Hepatitis B / Q000276:immunology / Q000628:therapy / Q000821:virology*; D006515:Hepatitis B virus; D006801:Humans; D015851:Interleukin-7 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008113:Liver Neoplasms / Q000276:immunology / Q000628:therapy / Q000821:virology*; D016031:Liver Transplantation; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020033:Protein Isoforms; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000821:virology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D055815:Young Adult
chemical_list: D015851:Interleukin-7; D020033:Protein Isoforms; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D017493:Leukocyte Common Antigens