title: Fabrication and cell affinity of biomimetic structured PLGA/articular cartilage ECM composite scaffold.
abstract: An ideal scaffold for cartilage tissue engineering should be biomimetic in not only mechanical property and biochemical composition, but also the morphological structure. In this research, we fabricated a composite scaffold with oriented structure to mimic cartilage physiological morphology, where natural nanofibrous articular cartilage extracellular matrix (ACECM) was used to mimic the biochemical composition, and synthetic PLGA was used to enhance the mechanical strength of ACECM. The composite scaffold has well oriented structure and more than 89% of porosity as well as about 107 μm of average pore diameter. The composite scaffold was compared with ACECM and PLGA scaffolds. Cell proliferation test showed that the number of MSCs in ACECM and composite scaffolds was noticeably bigger than that in PLGA scaffold, which was coincident with results of SEM observation and cell viability staining. The water absorption of ACECM and composite scaffolds were 22.1 and 10.2 times respectively, which was much higher than that of PLGA scaffolds (3.8 times). The compressive modulus of composite scaffold in hydrous status was 1.03 MPa, which was near 10 times higher than that of hydrous ACECM scaffold. The aforementioned results suggested that the composite scaffold has the potential for application in cartilage tissue engineering.
authors.affiliation: Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
authors.forename: Xifu
authors.initials: X
authors.lastname: Zheng
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Shenguo
authors.initials: S
authors.lastname: Wang
authors.forename: Shibi
authors.initials: S
authors.lastname: Lu
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhang
authors.forename: Shuyun
authors.initials: S
authors.lastname: Liu
authors.forename: Jingxiang
authors.initials: J
authors.lastname: Huang
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Wang
authors.forename: Baosheng
authors.initials: B
authors.lastname: Yin
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xu
authors.forename: Quanyi
authors.initials: Q
authors.lastname: Guo
mesh_terms: D000818:Animals; D032701:Biomimetics; D002358:Cartilage, Articular / Q000378:metabolism*; D049109:Cell Proliferation; D002470:Cell Survival; D005109:Extracellular Matrix / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008855:Microscopy, Electron, Scanning / Q000379:methods; D049329:Nanostructures / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011817:Rabbits; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid