title: Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle.
abstract: Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
authors.affiliation: Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Bush Farm Road, Roslin, Midlothian EH25 9RG, United Kingdom.
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Eckert
authors.forename: Francis
authors.initials: F
authors.lastname: Dziva
authors.forename: Roy R
authors.initials: RR
authors.lastname: Chaudhuri
authors.forename: Gemma C
authors.initials: GC
authors.lastname: Langridge
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Turner
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Pickard
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: Maskell
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Thomson
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial; D019453:Escherichia coli O157 / Q000235:genetics* / Q000378:metabolism*; D056084:Genetic Fitness; D005838:Genotype; D016254:Mutagenesis, Insertional; D010957:Plasmids
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial