title: The comparison of methylene blue and vitamin E in prevention of abdominal postoperative adhesion formation in rat uterine horn models: Biochemical and histopathologic evaluation.
abstract: OBJECTIVE
  To compare the effects of vitamin E and 1% methylen blue solutions on prevention of experimentally induced adhesions in rats.
  
  
  METHODS
  Thirty seven female Spraque Dawley rats were randomized into four groups. First group was kept as sham operated group. An adhesion model was constituted on the left uterine horn of the other groups. The lesion areas of rats from the second, the third and the fourth groups were coated with 2 ml 0.9 % saline solution (C group), 10 mg vitamin E (VE group) and 1% methylen blue solutions (MB group), respectively.
  
  
  RESULTS
  Histopathologically, adhesion scores, mononuclear cell infiltration, oedema and fibrosis were more prominent in the MB group compared with C and VE groups. There were no significant differences between the groups in tissue glutathione peroxidase (GPx), catalase (CAT) activities and glutation (GSH) level, these parameters were slightly increased in group with VE supplementation though. The administration of VE and MB significantly decreased NO (P<0.01) levels when compared to the C group. The level of malondialdehyde (MDA) in the VE group was significantly lower (P<0.05) than those of the Sh and C groups.
  
  
  CONCLUSIONS
  Intraperitoneal methylen blue solutions treatments were more effective according to vitamin E in preventing the formation of intra-abdominal adhesion in a rat uterine horn model.
authors.affiliation: Department of Obstetrics and Gynecology, Firat University, Elazig, Turkey. hamityildiz@firat.edu.tr
authors.forename: Hamit
authors.initials: H
authors.lastname: Yildiz
authors.forename: Ali Said
authors.initials: AS
authors.lastname: Durmus
authors.forename: Halil
authors.initials: H
authors.lastname: Simsek
authors.forename: Ihsan
authors.initials: I
authors.lastname: Yaman
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis; D005260:Female; D005978:Glutathione / Q000032:analysis; D005979:Glutathione Peroxidase / Q000032:analysis; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D008751:Methylene Blue / Q000627:therapeutic use*; D009569:Nitric Oxide / Q000032:analysis; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000627:therapeutic use; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D014591:Uterine Diseases / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D014810:Vitamin E / Q000627:therapeutic use*; D014815:Vitamins / Q000627:therapeutic use*
chemical_list: D014815:Vitamins; D014810:Vitamin E; D009569:Nitric Oxide; D012965:Sodium Chloride; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione; D008751:Methylene Blue