title: Integrated SPPS on continuous-flow radial microfluidic chip.
abstract: A novel integrated continuous-flow microfluidic system was designed and fabricated for solid phase peptide synthesis (SPPS) using conventional reactants. The microfluidic system was composed of a glass-based radial reaction chip, a diffluent chip, amino acid feeding reservoirs and continuous-flow reagent pathways. A tri-row cofferdam-fence structure was designed for solid phase supports trapping. Highly cross-linked, porous and high-loading 4-(hydroxymethyl)phenoxymethyl polystyrene (HMP) beads were prepared for microfluidic SPPS. The transfer losses, hazardous handling and time-consuming processes in traditional peptide cleavage steps were avoided by being replaced with the on-chip cleavage treatment. Six peptides from an antibody affinity peptide library against β-endorphin with different lengths and sequences were obtained simultaneously on the constructed continuous-flow microfluidic system within a short time. This microfluidic system is automatic, integrated, effective, low-cost, recyclable and environment-friendly for not only SPPS but also other solid phase chemical syntheses.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun North First Street 2, 100190 Beijing, PR China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Wang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Jizhong
authors.initials: J
authors.lastname: Liu
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Xie
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Xiong
authors.forename: Guoquan
authors.initials: G
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
mesh_terms: D000595:Amino Acid Sequence; D004867:Equipment Design; D005898:Glass / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D055616:Microtechnology; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D018511:Systems Integration
chemical_list: D010455:Peptides