title: Inhibitory effects of flavonoids from Abelmoschus manihot flowers on triglyceride accumulation in 3T3-L1 adipocytes.
abstract: The 95% EtOH extract from the flowers of Abelmoschus manihot (L.) Medic showed inhibitory activity on TG accumulation in 3T3-L1 preadipocyte. Chemical studies on the active fraction led to the isolation of 14 flavonoids (1-14). To clarify the multi-mechanism of the isolates on preadipocyte differentiation, the levels of TG and FFA and the related role transcription factors (PPARγ, CEBP/α, and ap2) expression were evaluated. At the concentration of 30 μM, compounds 1-6 and 10-14 showed inhibitory activity on TG accumulation significantly in mature 3T3-L1 cells. 1, 2, 4-7, 9, 10, 13, and 14 reduced the level of FFA. At the molecular level, the mRNA expressions of PPARγ, CEBP/α, and ap2 were down-regulated by compounds 1, 5, 9, 12, 13; 1-8, 10-14; and 1-4, 6, 8-12, 14, respectively. The structure-activity relationships of the 14 flavonoids were also discussed.
authors.affiliation: Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: An
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D028483:Abelmoschus / Q000737:chemistry*; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D013329:Structure-Activity Relationship; D050656:Transcription Factor AP-2 / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D005419:Flavonoids; D047495:PPAR gamma; D010936:Plant Extracts; D050656:Transcription Factor AP-2; D014280:Triglycerides