title: The DYW-E-PPR protein MEF14 is required for RNA editing at site matR-1895 in mitochondria of Arabidopsis thaliana.
abstract: We here identify the PPR protein MEF14 of the DYW subclass as a specific trans-factor required for C to U editing of site matR-1895 by genetic mapping of an EMS induced editing mutant in Arabidopsis thaliana. The wild type Col MEF14 gene complements mutant protoplasts. A T-DNA insertion in the MEF14 gene abolishes detectable editing at the matR-1895 site. Lack of RNA editing at the matR-1895 site does not alter the level of mature and precursor nad1 mRNA molecules. Such RNA editing mutants can be used to analyse the function of genes like this maturase related reading frame in plant mitochondria.
authors.affiliation: Molekulare Botanik, Universität Ulm, Ulm, Germany. daniil.verbitskiy@uni-ulm.de
authors.forename: Daniil
authors.initials: D
authors.lastname: Verbitskiy
authors.forename: Barbara
authors.initials: B
authors.lastname: Härtel
authors.forename: Anja
authors.initials: A
authors.lastname: Zehrmann
authors.forename: Axel
authors.initials: A
authors.lastname: Brennicke
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takenaka
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D030562:Databases, Protein; D023082:Defensins / Q000235:genetics / Q000378:metabolism; D004722:Endoribonucleases; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D056426:Genetic Loci; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D050505:Mutant Proteins / Q000378:metabolism; D009713:Nucleotidyltransferases; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D017393:RNA Editing; D012333:RNA, Messenger; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D023082:Defensins; C557043:MEF14 protein, Arabidopsis; D024101:Mitochondrial Proteins; D009153:Mutagens; D050505:Mutant Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D005020:Ethyl Methanesulfonate; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases