title: The role of the pyridoxine (vitamin B6) biosynthesis enzyme PDX1 in ultraviolet-B radiation responses in plants.
abstract: Ultraviolet-B radiation regulates plant growth and morphology at low and ambient fluence rates but can severely impact on plants at higher doses. Some plant UV-B responses are related to the formation of reactive oxygen species (ROS) and pyridoxine (vitamin B(6)) has been reported to be a quencher of ROS. UV-B irradiation of Arabidopsis Col-0 plants resulted in increased levels of PDX1 protein, compared with UV-A-exposed plants. This was shown by immunoblot analysis using specific polyclonal antibodies raised against the recombinant PDX1.3 protein and confirmed by mass spectrometry analysis of immunoprecipitated PDX1. The protein was located mainly in the cytosol but also to a small extent in the membrane fraction of plant leaves. Immunohistochemical analysis performed in pea revealed that PDX1 is present in UV-B-exposed leaf mesophyll and palisade parenchyma but not in epidermal cells. Pyridoxine production increased in Col-0 plants exposed to 3 days of UV-B, whereas in an Arabidopsis pdx1.3 mutant UV-B did not induce pyridoxine biosynthesis. In gene expression studies performed after UV-B exposure, the pdx1.3 mutant showed elevated transcript levels for the LHCB1*3 gene (encoding a chlorophyll a/b-binding protein of the photosystem II light-harvesting antenna complex) and the pathogenesis-related protein 5 (PR-5) gene, compared with wild type.
authors.affiliation: School of Science and Technology and Örebro Life Science Centre, Örebro University, Örebro, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Ristilä
authors.forename: Hilja
authors.initials: H
authors.lastname: Strid
authors.forename: Leif A
authors.initials: LA
authors.lastname: Eriksson
authors.forename: Ake
authors.initials: A
authors.lastname: Strid
authors.forename: Helena
authors.initials: H
authors.lastname: Sävenstrand
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019759:Carbon-Nitrogen Lyases; D002462:Cell Membrane / Q000528:radiation effects; D003600:Cytosol / Q000528:radiation effects; D015870:Gene Expression; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D019884:Nitrogenous Group Transferases / Q000235:genetics / Q000378:metabolism*; D018532:Pisum sativum / Q000201:enzymology* / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000502:physiology / Q000528:radiation effects; D018515:Plant Leaves / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011736:Pyridoxine / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011994:Recombinant Proteins; C053376:pathogenesis-related proteins, plant; D019884:Nitrogenous Group Transferases; D019759:Carbon-Nitrogen Lyases; C505161:At2g38230 protein, Arabidopsis; D011736:Pyridoxine