title: Detection of the glmM gene in Helicobacter pylori isolates with a novel primer by PCR.
abstract: A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori.
authors.affiliation: Escuela Nacional de Ciencias Biológicas-IPN, Departamento de Microbiología, Mexico, D.F., Mexico.
authors.forename: Maria Guadalupe Córdova
authors.initials: MG
authors.lastname: Espinoza
authors.forename: Rosa González
authors.initials: RG
authors.lastname: Vazquez
authors.forename: Iyari Morales
authors.initials: IM
authors.lastname: Mendez
authors.forename: Consuelo Ruelas
authors.initials: CR
authors.lastname: Vargas
authors.forename: Silvia Giono
authors.initials: SG
authors.lastname: Cerezo
mesh_terms: D000293:Adolescent; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007223:Infant; D010733:Phosphoglucomutase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014510:Urease / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D014510:Urease; C097631:phosphoglucosamine mutase; D010733:Phosphoglucomutase