title: [Detection of expanded-espectrum-betalactamases by DIRAMIC system: comparison with the double-disk synergy test and E-test].
abstract: The capacity of the DIRAMIC system to detect strains producing extended-spectrum-betalactamase (ESBL) was evaluated through the comparison with two phenotypic confirmatory tests: double-disk synergy test and E-test. Ninety seven clinical isolates of Escherichia coli y Klebsiella spp. previously characterized; not repeated and suspected of being ESBL producers were studied by the three methods. In comparison with E-test and double-disk synergy test, DIRAMIC system showed a sensitivity of 85.7% and 92.7% as well as specificity of 100% and 92.9%; respectively. The values found have a very high degree of concordance (kappa index > 0.80). The results obtained vouch for the utility of the DIRAMIC as a rapid method to alert about the presence of strains producing ESBL enzymes.
authors.affiliation: Escuela Latinoamericana de Medicina, La Habana, Cuba.
authors.forename: María
authors.initials: M
authors.lastname: Espino H
authors.forename: Estrella
authors.initials: E
authors.lastname: Alvarez V
authors.forename: Angela
authors.initials: A
authors.lastname: Zayas T
authors.forename: Rolando
authors.initials: R
authors.lastname: Contreras A
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D052978:Disk Diffusion Antimicrobial Tests; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests / Q000379:methods; D010641:Phenotype; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases