title: The terminal region of the E. coli chromosome localises at the periphery of the nucleoid.
abstract: BACKGROUND
  Bacterial chromosomes are organised into a compact and dynamic structures termed nucleoids. Cytological studies in model rod-shaped bacteria show that the different regions of the chromosome display distinct and specific sub-cellular positioning and choreographies during the course of the cell cycle. The localisation of chromosome loci along the length of the cell has been described. However, positioning of loci across the width of the cell has not been determined.
  
  
  RESULTS
  Here, we show that it is possible to assess the mean positioning of chromosomal loci across the width of the cell using two-dimension images from wide-field fluorescence microscopy. Observed apparent distributions of fluorescent-tagged loci of the E. coli chromosome along the cell diameter were compared with simulated distributions calculated using a range of cell width positioning models. Using this method, we detected the migration of chromosome loci towards the cell periphery induced by production of the bacteriophage T4 Ndd protein. In the absence of Ndd production, loci outside the replication terminus were located either randomly along the nucleoid width or towards the cell centre whereas loci inside the replication terminus were located at the periphery of the nucleoid in contrast to other loci.
  
  
  CONCLUSIONS
  Our approach allows to reliably observing the positioning of chromosome loci along the width of E. coli cells. The terminal region of the chromosome is preferentially located at the periphery of the nucleoid consistent with its specific roles in chromosome organisation and dynamics.
authors.affiliation: Université de Toulouse, Université Paul Sabatier, Laboratoire de Microbiologie et Génétique Moléculaires, F-31000 Toulouse, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Meile
authors.forename: Romain
authors.initials: R
authors.lastname: Mercier
authors.forename: Mathieu
authors.initials: M
authors.lastname: Stouf
authors.forename: Carine
authors.initials: C
authors.lastname: Pages
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Bouet
authors.forename: François
authors.initials: F
authors.lastname: Cornet
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D008856:Microscopy, Fluorescence