title: Preparative isolation of three anthraquinones from Rumex japonicus by high-speed counter-current chromatography.
abstract: Three anthraquinones--emodin, chrysophanol, and physcion--were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane-ethanol-water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by ¹H-NMR and ¹³C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.
authors.affiliation: College of Pharmacy, Jilin Medical College, Jilin 132013, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Ruonan
authors.initials: R
authors.lastname: Zhu
authors.forename: Jiankang
authors.initials: J
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D031804:Rumex / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000880:Anthraquinones; D012997:Solvents; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid