title: Abeta peptide toxicity is reduced after treatments decreasing phosphatidylethanolamine content in differentiated neuroblastoma cells.
abstract: We investigated whether the toxicity of oligomeric amyloid-beta peptide (Abeta1-42) upon differentiated human neuroblastoma SH-SY5Y cells, can be affected by changes of membrane lipid composition. An immunostaining technique, using lipids extracted from the cells and separated by thin layer chromatography, suggested that Abeta preferentially binds to phosphatidylethanolamine (PE), one of the major lipids in the cell extract. For this reason, we utilized treatments with putative inhibitors of phosphatidylethanolamine biosynthesis (choline, phosphocholine, R59949) to decrease its proportion in the cell membrane; choline treatment (2.5 mM, 24 h) showed the best performance, reducing phosphatidylethanolamine content from 5.7 to 3.3 μg phosphorous/mg protein. Either the extent of Abeta binding or its toxicity decreased onto choline-treated cells. These data may open the possibility to develop future strategies aiming to reduce Abeta toxicity in Alzheimer disease.
authors.affiliation: Department of Experimental Medicine, University of Milano Bicocca, Via Cadore, 48, 20052 Monza, Milan, Italy. emanuela.cazzaniga@unimib.it
authors.forename: Emanuela
authors.initials: E
authors.lastname: Cazzaniga
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bulbarelli
authors.forename: Elena
authors.initials: E
authors.lastname: Lonati
authors.forename: Antonina
authors.initials: A
authors.lastname: Orlando
authors.forename: Francesca
authors.initials: F
authors.lastname: Re
authors.forename: Maria
authors.initials: M
authors.lastname: Gregori
authors.forename: Massimo
authors.initials: M
authors.lastname: Masserini
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000502:physiology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D010714:Phosphatidylethanolamines; C075222:amyloid beta-protein (1-42); C483858:phosphatidylethanolamine