title: Effects of the hippocampal deep brain stimulation on cortical epileptic discharges in penicillin - induced epilepsy model in rats.
abstract: OBJECTIVE
  Experimental and clinical studies have revealed that hippocampal DBS can control epileptic activity, but the mechanism of action is obscure and optimal stimulation parameters are not clearly defined. The aim was to evaluate the effects of high frequency hippocampal stimulation on cortical epileptic activity in penicillin-induced epilepsy model.
  
  
  METHODS
  Twenty-five Sprague-Dawley rats were implanted DBS electrodes. In group-1 (n=10) hippocampal DBS was off and in the group-2 (n=10) hippocampal DBS was on (185 Hz, 0.5V, 1V, 2V, and 5V for 60 sec) following penicillin G injection intracortically. In the control group hippocampal DBS was on following 8 μl saline injection intracortically. EEG recordings were obtained before and 15 minutes following penicillin-G injection, and at 10th minutes following each stimulus for analysis in terms of frequency, amplitude, and power spectrum.
  
  
  RESULTS
  High frequency hippocampal DBS suppressed the acute penicillin-induced cortical epileptic activity independent from stimulus intensity. In the control group, hippocampal stimulation alone lead only to diffuse slowing of cerebral bioelectrical activity at 5V stimulation.
  
  
  CONCLUSIONS
  Our results revealed that continuous high frequency stimulation of the hippocampus suppressed acute cortical epileptic activity effectively without causing secondary epileptic discharges. These results are important in terms of defining the optimal parameters of hippocampal DBS in patients with epilepsy.
authors.affiliation: Pamukkale University, Faculty of Medicine, Department of Neurology, Denizli, Turkey.
authors.forename: Tarik
authors.initials: T
authors.lastname: Akman
authors.forename: Haydar
authors.initials: H
authors.lastname: Erken
authors.forename: Goksemin
authors.initials: G
authors.lastname: Acar
authors.forename: Elif
authors.initials: E
authors.lastname: Bolat
authors.forename: Zahir
authors.initials: Z
authors.lastname: Kizilay
authors.forename: Feridun
authors.initials: F
authors.lastname: Acar
authors.forename: Osman
authors.initials: O
authors.lastname: Genc
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000503:physiopathology*; D046690:Deep Brain Stimulation / Q000379:methods*; D004195:Disease Models, Animal; D004567:Electrodes, Implanted; D004569:Electroencephalography; D004827:Epilepsy / Q000139:chemically induced* / Q000175:diagnosis / Q000628:therapy*; D005260:Female; D006624:Hippocampus / Q000502:physiology*; D010400:Penicillin G / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010400:Penicillin G