title: Anticonvulsant profile of the alkaloids (+)-erythravine and (+)-11-α-hydroxy-erythravine isolated from the flowers of Erythrina mulungu Mart ex Benth (Leguminosae-Papilionaceae).
abstract: Neural mechanisms underlying the onset and maintenance of epileptic seizures involve alterations in inhibitory and/or excitatory neurotransmitter pathways. Thus, the prospecting of novel molecules from natural products that target both inhibition and excitation systems has deserved interest in the rational design of new anticonvulsants. We isolated the alkaloids (+)-erythravine and (+)-11-α-hydroxy-erythravine from the flowers of Erythrina mulungu and evaluated the action of these compounds against chemically induced seizures in rats. Our results showed that the administration of different doses of (+)-erythravine inhibited seizures evoked by bicuculline, pentylenetetrazole, and kainic acid at maximum of 80, 100, and 100%, respectively, whereas different doses of (+)-11-α-hydroxy-erythravine inhibited seizures at a maximum of 100% when induced by bicuculline, NMDA, and kainic acid, and, to a lesser extent, PTZ (60%). The analysis of mean latency to seizure onset of nonprotected animals, for specific doses of alkaloids, showed that (+)-erythravine increased latencies to seizures induced by bicuculline. Although (+)-erythravine exhibited very weak anticonvulsant action against seizures induced by NMDA, this alkaloid increased the latency in this assay. The increase in latency to onset of seizures promoted by (+)-11-α-hydroxy-erythravine reached a maximum of threefold in the bicuculline test. All animals were protected against death when treated with different doses of (+)-11-α-hydroxy-erythravine in the tests using the four chemical convulsants. Identical results were obtained when using (+)-erythravine in the tests of bicuculline, NMDA, and PTZ, and, to a lesser extent, kainic acid. Therefore, these data validate the anticonvulsant properties of the tested alkaloids, which is of relevance in consideration of the ethnopharmacological/biotechnological potential of E. mulungu.
authors.affiliation: Department of Biotechnology, University of Ribeirão Preto (UNAERP), São Paulo, Brazil.
authors.forename: Silmara Aparecida
authors.initials: SA
authors.lastname: Faggion
authors.forename: Alexandra Olimpio Siqueira
authors.initials: AO
authors.lastname: Cunha
authors.forename: Helene Aparecida
authors.initials: HA
authors.lastname: Fachim
authors.forename: Amanda Salomão
authors.initials: AS
authors.lastname: Gavin
authors.forename: Wagner Ferreira
authors.initials: WF
authors.lastname: dos Santos
authors.forename: Ana Maria Soares
authors.initials: AM
authors.lastname: Pereira
authors.forename: Renê Oliveira
authors.initials: RO
authors.lastname: Beleboni
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000627:therapeutic use*; D001640:Bicuculline / Q000633:toxicity; D016009:Chi-Square Distribution; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000737:chemistry / Q000627:therapeutic use*; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000633:toxicity; D008297:Male; D016202:N-Methylaspartate / Q000633:toxicity; D010433:Pentylenetetrazole / Q000633:toxicity; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: C517703:(+)-11alpha-hydroxyerythravine; C517704:(+)-erythravine; D000927:Anticonvulsants; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; D016202:N-Methylaspartate; D007608:Kainic Acid; D010433:Pentylenetetrazole; D001640:Bicuculline