title: Positive selection vector using the KillerRed gene.
abstract: Plasmid pZK18S is a novel positive selection vector containing the genetically encoded photosensitizer KillerRed as the selection marker. When transformed into host cells (lacI(q) or lacI(+)), the common medical surgical light is sufficient to activate phototoxicity of KillerRed. Thus, only the recombinants with disrupted reading frame of KillerRed genes finally allow forming viable colonies. Because lethality of KillerRed relies on light irradiation, no special host and culture medium are required to amplify and prepare pZK18S vector in larger quantities. The pZK18S was reliable and highly efficient for constructing the serial analysis of gene expression (SAGE) library.
authors.affiliation: Laboratory of Genetics and Molecular Biology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.forename: Dan
authors.initials: D
authors.lastname: Zou
authors.forename: Zaiyu
authors.initials: Z
authors.lastname: Li
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
authors.forename: Yanna
authors.initials: Y
authors.lastname: Zhou
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000737:chemistry / Q000302:isolation & purification; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008027:Light; D017319:Photosensitizing Agents / Q000737:chemistry
chemical_list: D015415:Biomarkers; D017319:Photosensitizing Agents; C508771:killer red protein, Anthomedusae; D049452:Green Fluorescent Proteins