title: Cytotoxic constituents from the skin of the toad Bufo bufo gargarizans.
abstract: To study the chemical composition of the skin of Bufo bufo gargarizans, various chromatographic methods were used in the isolation procedures and the structures of isolated compounds were determined based on NMR and MS analysis. As a result, two new compounds were isolated from its ethanolic extract and characterized as N-[2-hydroxy-2-(4-hydroxyphenyl)ethyl]-N-methylurea (1) and 19-oxocinobufotalin 3-adipoylarginine ester (2), together with 11 known compounds. Isolated bufadienolides showed significant inhibition effect against human hepatocellular carcinoma cell line SMMC-7721 in vitro.
authors.affiliation: Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Fu-Kai
authors.initials: FK
authors.lastname: Wu
authors.forename: Ying-Kun
authors.initials: YK
authors.lastname: Qiu
authors.forename: Hu-Yi
authors.initials: HY
authors.lastname: Zhao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.forename: Fa-Mei
authors.initials: FM
authors.lastname: Li
authors.forename: Yong-Tao
authors.initials: YT
authors.lastname: Jiang
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002025:Bufonidae; D002681:China; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008782:Methylurea Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012867:Skin / Q000737:chemistry*
chemical_list: C556207:9-oxocinobufotalin 3-adipoylarginine ester; D000970:Antineoplastic Agents; D002018:Bufanolides; D008782:Methylurea Compounds; C556206:N-(2-hydroxy-2-(4-hydroxyphenyl)ethyl)-N-methylurea; D001120:Arginine