title: In vitro silencing effect of chitosan nanoplexes containing siRNA expressing vector targeting VEGF in breast cancer cell lines.
abstract: Small interferring RNA (siRNA) is a powerful tool for controlling cellular processes of gene silencing at post-transcriptional level due to its high sequence-specific inhibition efficiency. The aim of this study is to compare silencing effect of chitosan/shVEGF (shRNA-expressing plasmid DNA targeting vascular endothelial growth factor-A (VEGF-A)) nanoplexes between four different cell lines, two of which are MCF-7 and MDA-MB435 breast cancer cell lines. Nanoplexes were prepared using different concentrations. The morphological and physicochemical characterization were made and silencing activity of appropriate formulations were investigated in vitro. As a result of in vitro transfection studies made in different cell lines with nanoplexes in the different ratios, the highest gene inhibition (60%) was measured in MCF-7 after transfection while the lowest gene inhibition (29%) was observed in MDA-MB435. This work suggests that RNA interference has potential to be applied to delivery system studies and to the angiogenesis treatment.
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Vocational Health School, Marmara University, Istanbul, Turkey. emine_salva@yahoo.com
authors.forename: E
authors.initials: E
authors.lastname: Salva
authors.forename: J
authors.initials: J
authors.lastname: Akbuğa
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004798:Enzymes / Q000097:blood / Q000737:chemistry; D005260:Female; D020868:Gene Silencing / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D044967:Serum / Q000737:chemistry; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D004798:Enzymes; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; D049452:Green Fluorescent Proteins; D048271:Chitosan