title: Variability in intron sequences of housekeeping and antigen-coding genes among Schistosoma japonicum isolates in mainland China.
abstract: The accurate characterization of Schistosoma japonicum has important implications for analyzing genetic variation and would provide basic data for disease control. Previous studies using proteins, coding sequences, and especially antigen-coding genes showed lower genetic variation among S. japonicum isolates from mainland China. Therefore, the present study focused on variations in intron sequences of housekeeping and antigen-coding genes, which may be more informative for genetic analysis. We compared sequence variation between introns of two housekeeping genes and two antigen-coding genes. All 4 genes were polymorphic among all the S. japonicum isolates in mainland China, with 103, 158, 47, and 19 polymorphic (segregating) sites per kilobase in intron sequences of Actin, FBPA, 22.6kDa antigen and GST-26, respectively. Introns of housekeeping genes were slightly more polymorphic than coding and non-coding regions of antigen-coding genes examined in the present study within or among lake/marshland and mountainous types. Phylogenetic analysis based on sequences of single gene or combined sequences of multiple genes showed no specific clustering comprising parasites from single geographical or endemic regions. These results demonstrated that introns of housekeeping and antigen-coding genes were polymorphic, but the intron sequences examined in the present study were not suitable markers for examining genetic relationship among different isolates from endemic regions in mainland China.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, PR China.
authors.forename: G H
authors.initials: GH
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: F C
authors.initials: FC
authors.lastname: Zou
authors.forename: J F
authors.initials: JF
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: M J
authors.initials: MJ
authors.lastname: Xu
authors.forename: Q
authors.initials: Q
authors.lastname: Lin
authors.forename: R Q
authors.initials: RQ
authors.lastname: Lin
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D017238:Genes, Helminth; D005819:Genetic Markers; D014644:Genetic Variation; D007438:Introns / Q000235:genetics*; D008297:Male; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000453:epidemiology / Q000469:parasitology; D017422:Sequence Analysis, DNA
chemical_list: D000947:Antigens, Helminth; D005819:Genetic Markers