title: Consolidated bioprocessing (CBP) performance of Clostridium phytofermentans on AFEX-treated corn stover for ethanol production.
abstract: Consolidated bioprocessing (CBP) is believed to be a potentially cost-efficient and commercially viable way to produce cellulosic biofuels. In this study, we have evaluated the performance of the CBP organism Clostridium phytofermentans (ATCC 700394) on AFEX-treated corn stover (AFEX-CS). Fermentation conditions including temperature, inoculation size, nutrients, and initial pH were investigated. At optimal conditions with 0.5% (w/w) glucan loading of AFEX-CS, C. phytofermentans hydrolyzed 76% of glucan and 88.6% of xylan in 10 days. These values reached 87% and 102% of those obtained by simultaneous saccharification and co-fermentation (SSCF) using commercial enzymes and S. cerevisiae 424A. Ethanol titer for CBP was found to be 2.8 g/L which was 71.8% of that yielded by SSCF (3.9 g/L). Decomposition products from AFEX-CS helped to increase ethanol yield somewhat during CBP. Particle size played a crucial role in the enhancement of sugar conversion by CBP.
authors.affiliation: Biomass Conversion Research Laboratory, Department of Chemical Engineering and Materials Science, Michigan State University, University Corporate Research Complex, 3900 Collins Road, Lansing, Michigan 48910, USA. jinmingj@egr.msu.edu
authors.forename: Mingjie
authors.initials: M
authors.lastname: Jin
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
authors.forename: Christa
authors.initials: C
authors.lastname: Gunawan
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Dale
mesh_terms: D003013:Clostridium / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000378:metabolism; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
chemical_list: D005936:Glucans; D000431:Ethanol