title: Fast cleanup system combined with a dioxin-responsive element-driven luciferase bioassay for analysis of polychlorinated dibenzo-p-dioxins/furans in sediments and soils.
abstract: A green technique was designed for assessing toxic equivalence (TEQ) levels of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in sediments and soil from dioxin-contaminated areas. This technique combines a fast cleanup system with a dioxin-responsive-element (DRE)-driven luciferase bioassay. Samples from sediment (n = 10) and soil (n = 11) were analyzed with the technique; levels of PCDD/Fs ranged from 75.1 to 2,670 ng DRE-driven luciferase activity (DL)-TEQ/kg dry weight (d.w.). Significant correlations were found between DL-TEQs (by the bioassay) and PCDD/F WHO-TEQs (by HRGC/HRMS). DL-TEQs is significantly correlated with WHO-TEQs of 2,3,7,8-TCDD and 1,2,3,7,8-PeCDD using a multiple stepwise linear regression model (adjusted R(2) = 0.962, p < 0.001).
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, Neipu 912, Pingtung County, Taiwan.
authors.forename: How-Ran
authors.initials: HR
authors.lastname: Chao
authors.forename: Ya-Fen
authors.initials: YF
authors.lastname: Wang
authors.forename: Ding-Yan
authors.initials: DY
authors.lastname: Lin
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Cheng
authors.forename: Tsui-Chun
authors.initials: TC
authors.lastname: Tsou
mesh_terms: D001572:Benzofurans / Q000032:analysis* / Q000737:chemistry; D001681:Biological Assay / Q000379:methods; D000072338:Dibenzofurans, Polychlorinated; D052244:Endocrine Disruptors / Q000032:analysis / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry; D055772:Green Chemistry Technology / Q000379:methods; D016014:Linear Models; D008156:Luciferases / Q000737:chemistry*; D015999:Multivariate Analysis; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D052244:Endocrine Disruptors; D000072317:Polychlorinated Dibenzodioxins; D012987:Soil; D012989:Soil Pollutants; D008156:Luciferases