title: Viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b.
abstract: The viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b (PCV-2b) were investigated. Seventy special pathogen free mice were divided into 2 groups with 35 mice in each group. The test group (TG) was infected with PCV-2b, the control group (CG) was inoculated with sterile cell cultures. Five mice in each group were sacrificed at 3, 7, 14, 21, 28, 35 and 42 dpi (day post infection), respectively. Necropsies were performed on all mice and tissues were collected for testing by histopathology, immunohistochemistry, transmission electron microscope (TEM) and polymerase chain reaction (PCR). Apoptosis and necrosis in lymphoid organs were observed in virus-infected mice, and became severe from 14 to 28 dpi. The proportion of PCV-2b antigen-positive cells was moderate in lung, heart, thymus, liver or kidney, and low in brain from TG. In spleen and cervical lymph node, the proportions of PCV-2b antigen-positive cells were low to high from 7 to 28 dpi, and moderate from 35 to 42 dpi. PCV-2b DNA was detected in all tissues examined in TG from 7 to 42 dpi. Viral inclusion bodies presented in the cytoplasm of lymphocytes, macrophages, hepatocytes, podocytes, neurocytes, spermatids and uterine epithelial cells in TG. In CG, no viruses and viral lesions were detected. PCV-2b could replicate in mice, and PCV-2b associated lesions in mice were similar to those observed in pigs. The present results indicate that it is possible to use Kunming mouse as an animal model for PMWS research.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, People's Republic of China.
authors.forename: Zhi-Bang
authors.initials: ZB
authors.lastname: Deng
authors.forename: Nai-Dong
authors.initials: ND
authors.lastname: Wang
authors.forename: Dao-Jun
authors.initials: DJ
authors.lastname: Xu
authors.forename: An-Wen
authors.initials: AW
authors.lastname: Yuan
authors.forename: Meng
authors.initials: M
authors.lastname: Ge
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Xue
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Yu
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D017209:Apoptosis; D018173:Circoviridae Infections / Q000276:immunology / Q000473:pathology* / Q000821:virology*; D017925:Circovirus / Q000276:immunology / Q000302:isolation & purification; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D051379:Mice; D009336:Necrosis / Q000821:virology; D016133:Polymerase Chain Reaction; D019282:Wasting Syndrome
chemical_list: D000956:Antigens, Viral