title: Biphasic tracheal relaxation induced by higenamine and nantenine from Nandina domestica Thunberg.
abstract: We compared the effects of the extract from fruits of Nandina domestica Thunberg (NDE) and its constituents, higenamine and nantenine, on contractile responses in isolated guinea-pig trachea. NDE (1 mg/ml) caused biphasic relaxation of the trachea precontracted with high-K(+) stimulation: the fast component was blocked by propranolol and mimicked by higenamine; and the slow was resistant to propranolol and mimicked by nantenine. Ca(2+)-induced contraction under high-K(+) stimulation was antagonized by nantenine or NDE + propranolol. These results suggest that NDE relaxes the trachea quickly through β-adrenoceptor stimulation by higenamine and slowly through Ca(2+) antagonism by nantenine.
authors.affiliation: Laboratory of Pharmacology, Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Ueki
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Akaishi
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Okumura
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Morioka
authors.forename: Kazuho
authors.initials: K
authors.lastname: Abe
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001060:Aporphines / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D005638:Fruit; D006168:Guinea Pigs; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D053827:Nandiniidae; D011188:Potassium / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D044005:Tetrahydroisoquinolines / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
chemical_list: D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D000470:Alkaloids; D001060:Aporphines; D044005:Tetrahydroisoquinolines; D011433:Propranolol; D011188:Potassium; D002118:Calcium; C012348:higenamine; C041615:nantenine