title: Synthesis, photophysical characterization, and enzymatic incorporation of a microenvironment-sensitive fluorescent uridine analog.
abstract: The synthesis of a microenvironment-sensitive base-modified fluorescent ribonucleoside analog based on a 5-(benzo[b]thiophen-2-yl)pyrimidine core, enzymatic incorporation of its corresponding triphosphate into RNA oligonucleotides, and photophysical characterization of fluorescently modified oligoribonucleotides are described.
authors.affiliation: Indian Institute of Science Education and Research, Pashan, Pune, India.
authors.forename: Maroti G
authors.initials: MG
authors.lastname: Pawar
authors.forename: Seergazhi G
authors.initials: SG
authors.lastname: Srivatsan
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D013876:Thiophenes / Q000138:chemical synthesis / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D014544:Uridine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D016376:Oligonucleotides, Antisense; D013876:Thiophenes; C088015:benzothiophene; D010165:Palladium; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate; D014529:Uridine