title: Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology.
abstract: NAC1, a BTB/POZ family member, has been suggested to participate in maintaining the stemness of embryonic stem cells and has been implicated in the pathogenesis of human cancer. In ovarian cancer, NAC1 upregulation is associated with disease aggressiveness and with the development of chemoresistance. Like other BTB/POZ proteins, NAC1 forms discrete nuclear bodies in non-dividing cells. To investigate the biological role of NAC1 nuclear bodies, we characterized the expression dynamics of NAC1 nuclear bodies during different phases of the cell cycle. Fluorescence recovery after photobleaching assays revealed that NAC1 was rapidly exchanged between the nucleoplasm and NAC1 nuclear bodies in interphase cells. The number of NAC1 bodies significantly increased and their size decreased in the S phase as compared to the G₀/G₁ and G₂ phases. NAC1 nuclear bodies disappeared and NAC1 became diffuse during mitosis. NAC1 nuclear bodies reappeared immediately after completion of mitosis. These results indicate that a cell cycle-dependent regulatory mechanism controls NAC1 body formation in the nucleus and suggest that NAC1 body dynamics are associated with mitosis or cytokinesis.
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Pei-Hsun
authors.initials: PH
authors.lastname: Wu
authors.forename: Shen-Hsiu
authors.initials: SH
authors.lastname: Hung
authors.forename: Tina
authors.initials: T
authors.lastname: Ren
authors.forename: Ie-Ming
authors.initials: IeM
authors.lastname: Shih
authors.forename: Yiider
authors.initials: Y
authors.lastname: Tseng
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000473:pathology / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D012097:Repressor Proteins / Q000032:analysis* / Q000378:metabolism*
chemical_list: C516477:NACC1 protein, human; D009363:Neoplasm Proteins; D012097:Repressor Proteins