title: The typing of Trypanosoma evansi isolates using mobile genetic element (MGE) PCR.
abstract: The mobile genetic element PCR (MGE-PCR) is a simple and sensitive technique that can be used to detect genetic variability in Trypanosoma brucei ssp. To investigate the reliability of MGE-PCR in genotyping Trypanosoma evansi, stocks that were isolated directly from camels and after their respective passage in mice were analyzed. Construction of a dendrogram using the MGE-PCR banding profiles revealed a clear distinction between T. evansi and T. brucei, as well as discriminating the T. evansi strains (T. evansi with minicircle types B and A). A minor host-dependent clustering shows a genetic difference of <15%. Changes in the banding profiles were observed after serial passage of T. evansi type B in mice, while those of T. evansi type A were identical. It is apparent that significant random insertion mobile element positional variation occurs when T. evansi isolates are introduced into a new host, a factor that needs to be considered when MGE-PCR is used to determine genetic variation in T. evansi isolates that have different host origins.
authors.affiliation: School of Veterinary Sciences, University of Queensland, Inner Ring Road, Gatton, 4343, Australia. z.njiru@uq.edu.au
authors.forename: Z K
authors.initials: ZK
authors.lastname: Njiru
authors.forename: P K
authors.initials: PK
authors.lastname: Gitonga
authors.forename: K
authors.initials: K
authors.lastname: Ndungu
mesh_terms: D000818:Animals; D002162:Camelus / Q000469:parasitology; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D051379:Mice; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D014345:Trypanosoma / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan