title: Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae.
abstract: Vibrio hollisae, a halophilic species recently reclassified as Grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. One important pathogenic Vibrio factor, thermostable direct haemolysin (TDH), has been purified and crystallized in two crystal forms using the vapour-diffusion method. The crystals belonged to an orthorhombic space group, with unit-cell parameters a = 104.8, b = 112.4, c = 61.3 Å and a = 122.9, b = 123.3, c = 89.8 Å. The crystals contained either four or eight molecules per asymmetric unit, with predicted solvent contents of 49.4 and 46.3% and Matthews coefficients (V(M)) of 2.4 and 2.3 Å(3) Da(-1), respectively. These crystals were suitable for structure determination, which would yield structural details related to the cytotoxicity and oligomeric structure of this pore-forming toxin.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan.
authors.forename: Yu-Kuo
authors.initials: YK
authors.lastname: Wang
authors.forename: Sheng-Cih
authors.initials: SC
authors.lastname: Huang
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Wu
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Chen
authors.forename: Wen-Hung
authors.initials: WH
authors.lastname: Chen
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Lin
authors.forename: Manoswini
authors.initials: M
authors.lastname: Nayak
authors.forename: Yan Ren
authors.initials: YR
authors.lastname: Lin
authors.forename: Thomas Tien-Hsiung
authors.initials: TT
authors.lastname: Li
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004058:Diffusion; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012015:Reference Standards; D017386:Sequence Homology, Amino Acid; D017356:Synchrotrons; D014733:Vibrio / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C068489:thermostable direct hemolysin