title: CEST MRI reporter genes.
abstract: In recent years, several reporter genes have been developed that can serve as a beacon for non-invasive magnetic resonance imaging (MRI). Here, we provide a brief summary of recent advances in MRI reporter gene technology, as well as detailed "hands-on" protocols for cloning, expression, and imaging of reporter genes based on chemical exchange saturation transfer (CEST).
authors.affiliation: Kennedy Krieger Institute, F.M. Kirby Research Center for Functional Brain Imaging, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. guanshu@mri.jhu.edu
authors.forename: Guanshu
authors.initials: G
authors.lastname: Liu
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003287:Contrast Media; D005455:Fluorescent Antibody Technique; D017930:Genes, Reporter / Q000235:genetics*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D051381:Rats; D015203:Reproducibility of Results; D014162:Transfection
chemical_list: D003287:Contrast Media