title: Distribution of HPV genotypes in women with cervical cancer in Auckland, New Zealand; a review of 50 specimens between 2000-2006.
abstract: BACKGROUND
  In New Zealand, around two hundred women are diagnosed with cervical cancer annually, with approximately seventy deaths from cervical cancer per year.
  
  
  OBJECTIVE
  Our aim was to determine the distribution of oncogenic HPV genotypes in biopsy specimens from women with diagnosed cervical cancers in the Auckland region of New Zealand between 2000-2006.
  
  
  METHODS
  Confirmed cases of cervical carcinoma were identified from the local pathology register, and representative tissue samples were taken from these blocks. Sections were deparaffinised, and DNA was extracted according to standard protocols. Samples were subject to PCR amplification using L1 consensus primer sets MY09/11 and GP5/6. Further type-specific amplification was performed on positive samples, using an in-house primer sequence based on target sequences within the E6 gene. Remaining samples were typed by a Linear Array Assay, or by DNA sequencing.
  
  
  RESULTS
  HPV DNA was detected in 100% of cases. In 49/50 samples, the HPV genotype was identified, with a total of 14 different HPV genotypes detectable. Together HPV-16 and 18 were found in 41/49 cases (83.6%) either singly or in combination.
  
  
  CONCLUSIONS
  Our findings suggest that the distribution of HPV genotypes in New Zealand is similar to that of other geographic areas.
  
  
  CONCLUSIONS
  Ongoing surveillance is warranted to ensure appropriate genotype selection for prophylactic HPV vaccinations.
authors.affiliation: Department of Virology and Immunology, Auckland City Hospital, Auckland, New Zealand. dwilliamson@adhb.govt.nz
authors.forename: Deborah
authors.initials: D
authors.lastname: Williamson
authors.forename: Radhika
authors.initials: R
authors.lastname: Nagappan
authors.forename: Rao
authors.initials: R
authors.lastname: Sirikonda
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Rahnama
authors.forename: Stephen
authors.initials: S
authors.lastname: Thomas
authors.forename: Margaret
authors.initials: M
authors.lastname: Lovell-Smith
authors.forename: Margaret
authors.initials: M
authors.lastname: Croxson
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009520:New Zealand; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000821:virology*
chemical_list: D004279:DNA, Viral