title: Inhibition of the adhesion to catheters of uropathogenic Escherichia coli by sub-inhibitory concentrations of cefotaxime.
abstract: OBJECTIVE
  To investigate the effect of sub-inhibitory concentrations of cefotaxime on adherence to siliconized latex urinary catheters of uropathogenic Escherichia coli strains from pregnant and non pregnant patients.
  
  
  METHODS
  Using random sampling, 30 E. coli strains were selected from hospitalized patients with catheter associated urinary tract infection, 12 from pregnant women and 18 from men and non-pregnant women. The strains were categorized on the basis of cefotaxime susceptibility, adhesion and biofilm production capacity, cell surface hydrophobicity and expression of adhesins and fimbriae in vitro.
  
  
  RESULTS
  The overall results indicated that sub-inhibitory concentrations of cefotaxime could reduce the adhesiveness, the biofilm production and hence, potentially, the infection rate associated with indwelling urinary catheters.
  
  
  CONCLUSIONS
  Based on our results, we propose that this reduction is due to decreasing exopolysaccharide production and increasing cell surface hydrophobicity of E.coli strains.
authors.affiliation: Area Bacteriología, Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario 2000, Santa Fe, Argentina. cbalague@fbioyf.unr.edu.ar
authors.forename: Claudia
authors.initials: C
authors.lastname: Balagué
authors.forename: Jorgelina
authors.initials: J
authors.lastname: Pérez
authors.forename: Mariángel
authors.initials: M
authors.lastname: Rinaudo
authors.forename: Luisa
authors.initials: L
authors.lastname: Fernández
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects; D055499:Catheter-Related Infections / Q000382:microbiology* / Q000517:prevention & control / Q000652:urine; D002439:Cefotaxime / Q000494:pharmacology*; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000517:prevention & control / Q000652:urine; D005260:Female; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007840:Latex / Q000737:chemistry; D008297:Male; D009994:Osmolar Concentration; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology / Q000517:prevention & control; D012826:Silicone Elastomers / Q000737:chemistry; D013499:Surface Properties / Q000187:drug effects; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000517:prevention & control / Q000652:urine; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D007840:Latex; D012826:Silicone Elastomers; D002439:Cefotaxime