title: Effect of bariatric surgery-induced weight loss on SR-BI-, ABCG1-, and ABCA1-mediated cellular cholesterol efflux in obese women.
abstract: OBJECTIVE
  We tested the hypothesis that quantitative changes in high-density lipoprotein (HDL) particles weight loss induced by Roux-en-Y bypass (RYGBP) in morbidly obese subjects might be associated with improved functionality of these particles in the reverse cholesterol transport pathway.
  
  
  RESULTS
  Thirty-four morbidly obese women were recruited and followed up before and 6 months after RYGBP. After surgery, along with a major weight loss (-20%; P < 0.0001), we observed a significant increase in HDL mass concentration (+14%; P < 0.04), reflecting a specific increase in large HDL2 subfraction levels (+42%; P < 0.01), whereas those of HDL3 remained unchanged. Cholesterol ester transfer protein activity decreased significantly (-15%; P < 0.0001). Efflux capacity of total plasma increased significantly via both scavenger receptor class B type I (SR-BI) (+58%; P < 0.0001) and ATP binding cassette G1 (ABCG1) (+26%; P < 0.0001) pathways. Such enhanced capacity resulted from increased capacity of HDL2 particles to mediate cholesterol efflux through the SR-BI pathway (+56%, P < 0.001) and from the increase plasma level of cholesteryl ester-rich HDL2 particles for the ABCG1 pathway.
  
  
  CONCLUSIONS
  RYGBP-induced weight loss results in improvement in atherogenic lipid profile including a shift toward a more cardioprotective HDL subfraction profile. In addition, our in vitro studies demonstrated an increased in plasma efflux capacity via both SR-BI and ABCG1 after surgery.
authors.affiliation: Assistance Publique-Hôpitaux de Paris, Endocrinology and Nutrition Department, Human Research Nutrition Center, Pitié-Salpêtrière Hospital, F-75013 Paris, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Aron-Wisnewsky
authors.forename: Zélie
authors.initials: Z
authors.lastname: Julia
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Bouillot
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: M John
authors.initials: MJ
authors.lastname: Chapman
authors.forename: Karine
authors.initials: K
authors.lastname: Clement
authors.forename: Maryse
authors.initials: M
authors.lastname: Guerin
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000502:physiology*; D000293:Adolescent; D000328:Adult; D000818:Animals; D050110:Bariatric Surgery / Q000534:rehabilitation; D016466:CHO Cells; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D051379:Mice; D008875:Middle Aged; D009767:Obesity, Morbid / Q000097:blood / Q000378:metabolism / Q000601:surgery*; D051381:Rats; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000502:physiology*; D015431:Weight Loss / Q000502:physiology*; D055815:Young Adult
chemical_list: C577836:ABCA1 protein, human; C419353:ABCG1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D002784:Cholesterol