title: In vitro Treg suppression assays.
abstract: Determining the activity of a regulatory T-cell population in vitro is often the first step in analyzing its function. To obtain reliable and reproducible results, it is critical to follow the protocol that is most applicable to your experimental question. We have outlined below a basic in vitro suppression assay as well as a variety of alternative/additional protocols that can be utilized alone or in combination as desired.
authors.affiliation: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Lauren W
authors.initials: LW
authors.lastname: Collison
authors.forename: Dario A A
authors.initials: DA
authors.lastname: Vignali
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D003584:Cytological Techniques; D008213:Lymphocyte Activation; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology*