title: Mannitol delivery by vibrating mesh nebulisation for enhancing mucociliary clearance.
abstract: Mucociliary clearance is compromised by airway surface liquid dehydration in respiratory disease states such as cystic fibrosis. Rehydration by hyperosmolar agents such as nebulised hypertonic saline and dry powder mannitol has demonstrated in vivo safety and efficacy for restoring mucociliary function. Mannitol, delivered as a nebulised formulation for this purpose, has not been investigated as yet. The current study examines the feasibility of delivering such a formulation using recent vibrating mesh technology. Nebulisation was conducted using an Aeroneb Go(TM) vibrating mesh nebuliser, and aerosol size was assessed by laser diffraction. Cascade impaction coupled with mass assay by high-performance liquid chromatography was used to confirm fluid uniformity and correlation with laser diffraction sizing. The following nebuliser formulations were prepared and aerosolised: deionised water, mannitol (150 mg/mL) aqueous solution, sodium chloride aqueous solution [0.2%, 1%, 3%, 5%, 7% (w/v)] and mannitol (150 mg/mL) in sodium chloride solution [0.2%, 1%, 3%, 7% (w/v)]. Mannitol aqueous solution was poorly nebulised, resulting in lengthy treatment times and large median droplet size. Addition of sodium chloride drastically improved nebuliser performance and aerosol characteristics. In vivo studies are necessary to confirm efficacy of nebulised mannitol. If substantiated, it could provide a pleasant-tasting alternative mucoactive agent with prolonged therapeutic action.
authors.affiliation: Advanced Drug Delivery Group, Faculty of Pharmacy, The University of Sydney, Sydney, New South Wales 2006, Australia.
authors.forename: John Gar Yan
authors.initials: JG
authors.lastname: Chan
authors.forename: Philip Chi Lip
authors.initials: PC
authors.lastname: Kwok
authors.forename: Paul M
authors.initials: PM
authors.lastname: Young
authors.forename: Hak-Kim
authors.initials: HK
authors.lastname: Chan
authors.forename: Daniela
authors.initials: D
authors.lastname: Traini
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000704:Analysis of Variance; D000818:Animals; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004867:Equipment Design; D005240:Feasibility Studies; D006801:Humans; D008353:Mannitol / Q000008:administration & dosage* / Q000737:chemistry; D009079:Mucociliary Clearance / Q000187:drug effects*; D009330:Nebulizers and Vaporizers; D010316:Particle Size; D012031:Refractometry; D012965:Sodium Chloride / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D014732:Vibration
chemical_list: D000336:Aerosols; D008353:Mannitol; D012965:Sodium Chloride