title: [Effect of bone-marrow mesenchymal stem cells on the immune function of aging rats].
abstract: OBJECTIVE
  To investigate the effect of transplantation of bone-marrow mesenchymal stem cells (MSCs) on the immune functions of aging rats.
  
  
  METHODS
  Healthy SD rats were randomized into normal control, aging model group and MSCs group. The aging model was established by daily subcutaneous injection of D-galactose for 4 consecutive months. MSCs were isolated from the bone marrow of adult SD rats and injected (3×10(6) MSCs) in rats in the MSCs group via the tail vein once a week for 4 weeks. The spleen index, activity of T lymphocytes and the levels of IL-2 and IL-10 in spleen were measured, and the pathological changes of the spleen were observed after the treatments.
  
  
  RESULTS
  MSCs transplantation enhanced the cellular immune function of aging rats manifested by obviously increased spleen index, activity of T lymphocyte and the level of IL-2, and lowered level of IL-10 in the spleen. The rats in the aging model group showed serious spleen injury, which was obviously lessened by MSCs injection.
  
  
  CONCLUSIONS
  MSCs transplantation can improve the cellular immune function of aging rats and ameliorate spleen injury induced by D-galactose.
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zhihongwang1980@126.com
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: Kun-yuan
authors.initials: KY
authors.lastname: Guo
authors.forename: Yu-lian
authors.initials: YL
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Xue-yun
authors.initials: XY
authors.lastname: Zhou
authors.forename: Qing-mei
authors.initials: QM
authors.lastname: He
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D005260:Female; D005690:Galactose / Q000009:adverse effects; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007376:Interleukin-2; D016753:Interleukin-10; D005690:Galactose