title: Angiogenic activity of Calendula officinalis flowers L. in rats.
abstract: OBJECTIVE
  In this work, angiogenic activity of Calendula officinalis L. (Asteraceae) ethanolic extract and dichloromethane and hexanic fractions were evaluated, considering medicinal properties, especially healing activity, are attributed to this plant.
  
  
  METHODS
  Models using 36 rats and 90 embryonated eggs were used to evaluate healing and angiogenic activities of extracts and fractions of the plant, through the induction of skin wounds and the chorioallantoic membrane, respectively. The effect of vascular proliferation was also tested from the study to verify the intensity of expression of vascular endothelial growth factor (VEGF) in cutaneous wounds in rats.
  
  
  RESULTS
  The angiogenic activity of the extract and the fractions was evidenced in both experimental models. It was verified that this effect is not directly related to the expression of VEGF and it could be associated to other pro-angiogenic factors.
  
  
  CONCLUSIONS
  The healing activity referred to C. officinalis is related, among other factors, to its positive effect on angiogenesis, characterized by the induction of neovascularization.
authors.affiliation: Department of Pathology, UFG, Goias, Brazil. lathosvet@hotmail.com
authors.forename: Leila Maria Leal
authors.initials: LM
authors.lastname: Parente
authors.forename: Maria Auxiliadora
authors.initials: MA
authors.lastname: Andrade
authors.forename: Luiz Augusto Batista
authors.initials: LA
authors.lastname: Brito
authors.forename: Veridiana Maria Brianezi Dignani de
authors.initials: VM
authors.lastname: Moura
authors.forename: Marina Pacheco
authors.initials: MP
authors.lastname: Miguel
authors.forename: Ruy de Souza
authors.initials: Rde S
authors.lastname: Lino-Júnior
authors.forename: Leonice Faustino Manrique
authors.initials: LF
authors.lastname: Tresvenzol
authors.forename: José Realino de
authors.initials: JR
authors.lastname: Paula
authors.forename: Neusa Margarida
authors.initials: NM
authors.lastname: Paulo
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D020838:Calendula / Q000737:chemistry*; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D005260:Female; D035264:Flowers / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000098:blood supply / Q000293:injuries / Q000378:metabolism; D018709:Statistics, Nonparametric; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D043925:Angiogenesis Inducing Agents; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A