title: Diversity of aromatic ring-hydroxylating dioxygenase gene in Citrobacter.
abstract: Genetic and functional diversity of Citrobacter spp. for their abilities to degrade aromatic compounds was evaluated to develop mixed cultures or a consortium for bioremediation technology. Thirty Citrobacter strains isolated from various effluent treatment plants were found to degrade a range of aromatic compounds: phenol, benzoate, hydroxy benzoic acid and biotransform mono-chlorophenols and di-chlorophenol within 24 to 48 h of incubation at 30 °C. Sequence similarity and phylogeny of the ARHD gene transcripts (730 nucleotides) depicted their diversity within 9 Citrobacter strains: HPC255, HPC369, HPC560, HPC570, HPC784, HPC1196, HPC1216, HPC1276 and HPC1299. Here, the degree of associations varied up to 84% with (i) ARHD α-sub unit (SU), (ii) LSU of Phenylpropionate dioxygenase (PDO), (iii) Phenol hydroxylase α-SU, (iv) Benzoate 1,2-dioxygenase, α-SU, (v) Naphthalene dioxygenase LSU, etc. This study has provided basic information, which can be used to develop a consortium of bacteria with mutually beneficial characteristics.
authors.affiliation: Environmental Genomics Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nehru Marg, Nagpur 440 020, MH, India.
authors.forename: S
authors.initials: S
authors.lastname: Selvakumaran
authors.forename: Atya
authors.initials: A
authors.lastname: Kapley
authors.forename: Sanjay M
authors.initials: SM
authors.lastname: Kashyap
authors.forename: Hatim F
authors.initials: HF
authors.lastname: Daginawala
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Kalia
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
mesh_terms: D001483:Base Sequence; D002954:Citrobacter / Q000201:enzymology* / Q000235:genetics; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics* / Q000378:metabolism; D052918:Environmental Restoration and Remediation / Q000379:methods*; D014644:Genetic Variation; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D006900:Hydroxylation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017931:DNA Primers; D006841:Hydrocarbons, Aromatic; D049308:Dioxygenases