title: Chemometric analysis of lipase-catalyzed synthesis of xylitol esters in a solvent-free system.
abstract: Immobilized Candida antarctica lipase B-catalyzed esterification of xylitol and two fatty acids (capric and caproic acid) were studied in a solvent-free system. The Taguchi orthogonal array method based on three-level-four-variables with nine experiments was applied for the analysis and optimization of the reaction parameters including time, substrate molar ratio, amount of enzyme, and amount of molecular sieve. The obtained conversion was higher in the esterification of xylitol and capric acid with longer chain length. The optimum conditions derived via the Taguchi approach for the synthesis of xylitol caprate and xylitol caproate were reaction time, 29 and 18h; substrate molar ratio, 0.3 and 1.0; enzyme amount, 0.20 and 0.05g, and molecular sieve amount of 0.03g, respectively. The good correlation between the predicted conversions (74.18% and 61.23%) and the actual values (74.05% and 60.5%) shows that the model derived from the Taguchi orthogonal array can be used for optimization and better understanding of the effect of reaction parameters on the enzymatic synthesis of xylitol esters in a solvent-free system.
authors.affiliation: Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Atena
authors.initials: A
authors.lastname: Adnani
authors.forename: Mahiran
authors.initials: M
authors.lastname: Basri
authors.forename: Naz
authors.initials: N
authors.lastname: Chaibakhsh
authors.forename: Hossein Abbastabar
authors.initials: HA
authors.lastname: Ahangar
authors.forename: Abu Bakar
authors.initials: AB
authors.lastname: Salleh
authors.forename: Raja Noor Zaliha Raja Abdul
authors.initials: RN
authors.lastname: Rahman
authors.forename: Mohd Basyaruddin
authors.initials: MB
authors.lastname: Abdul Rahman
mesh_terms: D002208:Caproates / Q000138:chemical synthesis* / Q000737:chemistry; D002384:Catalysis; D003652:Decanoic Acids / Q000737:chemistry*; D045703:Emulsifying Agents / Q000138:chemical synthesis*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D008956:Models, Chemical; D013501:Surface-Active Agents / Q000138:chemical synthesis*; D013696:Temperature; D014993:Xylitol / Q000737:chemistry*
chemical_list: D002208:Caproates; D003652:Decanoic Acids; D045703:Emulsifying Agents; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D013501:Surface-Active Agents; C037652:hexanoic acid; C031071:decanoic acid; D008049:Lipase; C431349:lipase B, Candida antarctica; D014993:Xylitol