title: [Comparison of mtDNA extraction from different parts of sarcosaphagous insects].
abstract: OBJECTIVE
  To explore mitochondrial DNA (mtDNA) extraction effects of different parts from sarcosaphagous insects using improved cetyltriethylammnonium bromide (CTAB) method.
  
  
  METHODS
  Thirteen Lucilia sericata (Meigen) and 13 Nicrophorus fossor (Erichson) were collected from the corpses of rabbits placed on the outdoor lawn in Huhehot district. Four parts (head, chest muscle, legs and wings) of insect were collected, and the mtDNA of all samples were extracted using CTAB method. The purity and concentration were tested using protein and nucleic acid spectrophotometry. The integrity of the extracted mtDNA and PCR products were checked by agarose gel electrophoresis. The PCR products were sequenced and the obtained sequences were imputed into GenBank for comparison.
  
  
  RESULTS
  mtDNA were successfully extracted from 10 head samples, 6 legs samples, 4 wing samples and 13 chest muscle samples of the Lucilia sericata (Meigen). Also, mtDNA were successfully extracted from 5 head samples, 8 legs samples, 3 wing samples and 13 chest muscle samples of the Nicrophorus fossor (Erichson).
  
  
  CONCLUSIONS
  mtDNA can be obtained from chest muscle and other parts of sarcosaphagous insects using the improved CTAB method.
authors.affiliation: Department of Forensic Medicine, School of Basic Medical Science, Central South University, Changsha 410013, China. gdy82@126.com
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Guo
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Cai
authors.forename: Ri-Na
authors.initials: RN
authors.lastname: Su
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Chang
authors.forename: Ling-Mei
authors.initials: LM
authors.lastname: Lan
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Ji-Fang
authors.initials: JF
authors.lastname: Wen
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004772:Entomology; D005554:Forensic Medicine / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV