title: Histological and immunohistochemical identification of atypical ductal mammary hyperplasia as a preneoplastic marker in dogs.
abstract: This study describes and evaluates the morphological and molecular relationship between canine mammary ductal hyperplasias with atypia and canine mammary neoplasias. Ductal hyperplasia was identified in association with malignant neoplasia in 56 of the 115 cases (48,8%), and although ductal hyperplasia without atypia was the type most frequently noted in the cases, most examples of hyperplasia with atypia were associated with mammary tumors. Estrogen receptor, E-cadherin, and cytokeratins 1, 5, 10 and 14 (CK34bE12) expression was quite lower than in normal mammary tissue, and HER2 overexpression was absent in all proliferative cells of ductal hyperplasia. The Ki-67 expression, epidermal growth factor receptor and progesterone receptor expression appeared higher in those hyperplastic lesions analyzed than in normal mammary glands. These findings suggest that canine mammary atypical hyperplasia may play an important role in the process of malignant neoplastic transformation, with molecular alterations that are similar to precursor lesions reported in humans.
authors.affiliation: Laboratory of Comparative Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, MG, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Ferreira
authors.forename: H
authors.initials: H
authors.lastname: Gobbi
authors.forename: B S
authors.initials: BS
authors.lastname: Saraiva
authors.forename: G D
authors.initials: GD
authors.lastname: Cassali
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006965:Hyperplasia / Q000473:pathology / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D019394:Ki-67 Antigen / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000473:pathology*; D015674:Mammary Neoplasms, Animal / Q000473:pathology*; D011230:Precancerous Conditions / Q000473:pathology / Q000662:veterinary*; D011980:Receptors, Progesterone / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D011980:Receptors, Progesterone; D066246:ErbB Receptors