title: Field detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR.
abstract: A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.
authors.affiliation: Department of Environmental Health, Rollins School of Public Health, Emory University, 1518 Clifton Rd. NE, Atlanta, GA 30322, USA.
authors.forename: Caitlin
authors.initials: C
authors.lastname: Worrell
authors.forename: Ning
authors.initials: N
authors.lastname: Xiao
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Vidal
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Zhong
authors.forename: Justin
authors.initials: J
authors.lastname: Remais
mesh_terms: D000818:Animals; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012549:Schistosoma japonicum / Q000145:classification / Q000235:genetics*; D014867:Water / Q000469:parasitology
chemical_list: D014867:Water