title: No evidence of association between 118A>G OPRM1 polymorphism and heroin dependence in a large Bulgarian case-control sample.
abstract: The μ-opioid receptor is the primary site of action of most opioids. The 118A>G (rs1799971) polymorphism in exon 1 of the μ-opioid receptor gene (OPRM1) leads to an Asn40Asp amino acid change that affects a putative N-glycosylation site. It has been widely investigated for association with alcohol and drug dependence and pain sensitivity, with mixed results. The aim of the current study was to examine whether this polymorphism was associated with heroin dependence in a large Bulgarian cohort of 1842 active users and 1451 population controls. SNP genotyping was done using Real-Time PCR TaqMan technology. Association analyses were conducted, separately for Roma and non-Roma participants. Our results suggest that there is no direct effect of 118A>G genotype on the risk for heroin dependence among active heroin users.
authors.affiliation: Molecular Medicine Center and Department of Medical Chemistry and Biochemistry, Medical University - Sofia, Sofia 1431, Bulgaria.
authors.forename: Momchil A
authors.initials: MA
authors.lastname: Nikolov
authors.forename: Olga
authors.initials: O
authors.lastname: Beltcheva
authors.forename: Antoaneta
authors.initials: A
authors.lastname: Galabova
authors.forename: Anna
authors.initials: A
authors.lastname: Ljubenova
authors.forename: Elena
authors.initials: E
authors.lastname: Jankova
authors.forename: Galin
authors.initials: G
authors.lastname: Gergov
authors.forename: Atanas A
authors.initials: AA
authors.lastname: Russev
authors.forename: Michael T
authors.initials: MT
authors.lastname: Lynskey
authors.forename: Elliot C
authors.initials: EC
authors.lastname: Nelson
authors.forename: Eleonora
authors.initials: E
authors.lastname: Nesheva
authors.forename: Dorita
authors.initials: D
authors.lastname: Krasteva
authors.forename: Philip
authors.initials: P
authors.lastname: Lazarov
authors.forename: Vanio I
authors.initials: VI
authors.lastname: Mitev
authors.forename: Ivo M
authors.initials: IM
authors.lastname: Kremensky
authors.forename: Radka P
authors.initials: RP
authors.lastname: Kaneva
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Todorov
mesh_terms: D000328:Adult; D000483:Alleles; D000701:Analgesics, Opioid / Q000378:metabolism; D002031:Bulgaria / Q000453:epidemiology / Q000208:ethnology; D016022:Case-Control Studies; D005091:Exons; D005260:Female; D005838:Genotype; D006556:Heroin Dependence / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D017450:Receptors, Opioid, mu / Q000235:genetics* / Q000502:physiology; D012307:Risk Factors; D012383:Romania / Q000208:ethnology
chemical_list: D000701:Analgesics, Opioid; C485764:OPRM1 protein, human; D017450:Receptors, Opioid, mu