title: Development of a sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay (ic-ELISA) based on the monoclonal antibody for the detection of the imidaclothiz residue.
abstract: An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on monoclonal antibodies (MoAbs) for imidaclothiz was developed. The hapten of imidaclothiz was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the artificial antigens. MoAbs were obtained by immunizing BALB/c mice. Under the optimized conditions (10% methanol, 0.14 M Na(+), and pH 7.4), the half-maximal inhibition concentration (IC(50)) was 0.0875 ± 0.0034 mg/L and the limit of detection (IC(20)) was 0.0178 ± 0.0018 mg/L for imidaclothiz. There were no obvious cross-reactivities with most of the structural analogues of neonicotinoid insecticides, except imidacloprid. The recoveries of imidaclothiz in environmental and agricultural samples, including tap water, paddy water, soil, and cabbage, ranged from 80.43 to 113.83%, well within the requirements of residue detection. These results showed that this immunoassay could be used for the determination of imidaclothiz in environmental and agricultural samples.
authors.affiliation: Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University , Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: Fang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Ke-Wei
authors.initials: KW
authors.lastname: Ren
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Cao
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Shi
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010573:Pesticide Residues / Q000032:analysis*; D012987:Soil / Q000032:analysis; D013844:Thiazoles / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D010573:Pesticide Residues; D012987:Soil; D013844:Thiazoles; D014867:Water; C550087:imidaclothiz