title: Mutation detection and accurate diagnosis of extensively drug-resistant tuberculosis: report from a tertiary care center in India.
abstract: We screened and spoligotyped 150 consecutive phenotypically confirmed extensively drug-resistant Mycobacterium tuberculosis (XDR-TB) isolates (January 2008 to March 2009) for rifampin, isoniazid, fluoroquinolone, and aminoglycoside resistance targeting rpoB, inhA, katG, gyrA, gyrB, and rrs. Mutations predominant among XDR-TB were S315T (katG) (100% of isolates), S531L (rpoB) (97% of isolates), D94G (gyrA) (53% of isolates), and A1401G (rrs) (71% of isolates). Spoligotyping revealed 62% of the isolates to be Beijing.
authors.affiliation: Department of Research, P. D. Hinduja National Hospital and Medical Research Centre, Veer Savarkar Marg, Mahim, Mumbai 400016, India.
authors.forename: Kanchan
authors.initials: K
authors.lastname: Ajbani
authors.forename: Camilla
authors.initials: C
authors.lastname: Rodrigues
authors.forename: Shubhada
authors.initials: S
authors.lastname: Shenai
authors.forename: Ajita
authors.initials: A
authors.lastname: Mehta
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D024901:Drug Resistance, Multiple, Bacterial; D054908:Extensively Drug-Resistant Tuberculosis / Q000175:diagnosis* / Q000382:microbiology; D005838:Genotype; D006801:Humans; D007194:India; D058889:Molecular Typing; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins