title: Plasmodium vivax tryptophan-rich antigen PvTRAg33.5 contains alpha helical structure and multidomain architecture.
abstract: Tryptophan-rich proteins from several malarial parasites have been identified where they play an important role in host-parasite interaction. Structural characterization of these proteins is needed to develop them as therapeutic targets. Here, we describe a novel Plasmodium vivax tryptophan-rich protein named PvTRAg33.5. It is expressed by blood stage(s) of the parasite and its gene contains two exons. The exon 1 encodes for a 23 amino acids long putative signal peptide which is likely to be cleaved off whereas the exon 2 encodes for the mature protein of 252 amino acids. The mature protein contains B-cell epitopes which were recognized by the human immune system during P.vivax infection. The PvTRAg33.5 contains 24 (9.5%) tryptophan residues and six motifs whose patterns were similar among tryptophan-rich proteins. The modeled structure of the PvTRAg33.5 consists of a multidomain architecture which is stabilized by the presence of large number of tryptophan residues. The recombinant PvTRAg33.5 showed predominantly α helical structure and alpha helix to beta sheet transition at pH below 4.5. Protein acquires an irreversible non-native state at temperature more than 50°C at neutral pH. Its secondary and tertiary structures remain stable in the presence of 35% alcohol but these structures are destabilized at higher alcohol concentrations due to the disturbance of hydrophobic interactions between tryptophanyl residues. These structural changes in the protein might occur during its translocation to interact with other proteins at its final destination for biological function such as erythrocyte invasion.
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Hema
authors.initials: H
authors.lastname: Bora
authors.forename: Sheena
authors.initials: S
authors.lastname: Garg
authors.forename: Priyankar
authors.initials: P
authors.lastname: Sen
authors.forename: Deepak
authors.initials: D
authors.lastname: Kumar
authors.forename: Punit
authors.initials: P
authors.lastname: Kaur
authors.forename: Rizwan Hasan
authors.initials: RH
authors.lastname: Khan
authors.forename: Yagya D
authors.initials: YD
authors.lastname: Sharma
mesh_terms: D000953:Antigens, Protozoan / Q000737:chemistry*; D001692:Biological Transport; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007109:Immunity; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000737:chemistry* / Q000276:immunology; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000953:Antigens, Protozoan; D018985:Epitopes, B-Lymphocyte; D015800:Protozoan Proteins; D014364:Tryptophan