title: Poly(β-amino amine) cross-linked PEIs as highly efficient gene vectors.
abstract: To increase the release of DNA into the cytoplasm and further improve transgene expression of nucleic acid novel polymeric gene carriers were prepared which would be biodegradable under the reducing conditions in the cytoplasm. Disulfide-containing poly(β-amino amine)s were first synthesized and then used to cross-link low molecular weight polyethyleneimine (1800 Da) through Michael addition to obtain SS-PBAA-PEIs as the final gene carriers. The physicochemical characteristics of SS-PBAA-PEI/DNA complexes were characterized. In vitro transfection mediated by the SS-PBAA-PEIs under serum conditions was carried out. Cell uptake of the gene delivery systems was observed by confocal laser scanning microscopy. The results of the physicochemical characterisation demonstrated that the SS-PBAA-PEIs could efficiently condense DNA. In vitro transfection under serum conditions showed that SS-PBAA-PEIs had comparable or even higher transfection efficiencies than 25 kDa PEI. And SS-PBAA-PEIs showed much lower cytotoxicity compared with 25 kDa PEI. In summary, the SS-PBAA-PEIs possess great potential as non-viral gene vectors and exhibit high transfection efficiency under serum conditions.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, People's Republic of China.
authors.forename: Ji-Zhe
authors.initials: JZ
authors.lastname: Deng
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Sun
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Wang
authors.forename: Cao
authors.initials: C
authors.lastname: Li
authors.forename: Fu-Wei
authors.initials: FW
authors.lastname: Huang
authors.forename: Si-Xue
authors.initials: SX
authors.lastname: Cheng
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D000588:Amines / Q000737:chemistry*; D002021:Buffers; D016923:Cell Death; D002470:Cell Survival; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D024202:Electrophoretic Mobility Shift Assay; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D055672:Static Electricity; D017186:Titrimetry; D014162:Transfection
chemical_list: D000588:Amines; D002021:Buffers; D003432:Cross-Linking Reagents; D004220:Disulfides; D011073:Polyamines; D011094:Polyethyleneimine; D008156:Luciferases