title: Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
abstract: The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.
authors.affiliation: Broad Institute, Cambridge, MA 02142, USA.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bock
authors.forename: Evangelos
authors.initials: E
authors.lastname: Kiskinis
authors.forename: Griet
authors.initials: G
authors.lastname: Verstappen
authors.forename: Hongcang
authors.initials: H
authors.lastname: Gu
authors.forename: Gabriella
authors.initials: G
authors.lastname: Boulting
authors.forename: Zachary D
authors.initials: ZD
authors.lastname: Smith
authors.forename: Michael
authors.initials: M
authors.lastname: Ziller
authors.forename: Gist F
authors.initials: GF
authors.lastname: Croft
authors.forename: Mackenzie W
authors.initials: MW
authors.lastname: Amoroso
authors.forename: Derek H
authors.initials: DH
authors.lastname: Oakley
authors.forename: Andreas
authors.initials: A
authors.lastname: Gnirke
authors.forename: Kevin
authors.initials: K
authors.lastname: Eggan
authors.forename: Alexander
authors.initials: A
authors.lastname: Meissner
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000502:physiology*; D020869:Gene Expression Profiling / Q000592:standards*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology*