title: Laser-guided cell micropatterning system.
abstract: Employing optical force, our laser-guided cell micropatterning system, is capable of patterning different cell types onto and within standard cell research devices, including commercially available multielectrode arrays (MEAs) with glass culture rings, 35 mm Petri dishes, and microdevices microfabricated with polydimethylsiloxane on 22 mm × 22 mm cover glasses. We discuss the theory of optical forces for generating laser guidance and the calculation of optimal beam characteristics for cell guidance. We describe the hardware design and software program for the cell patterning system. Finally, we demonstrate the capabilities of the system by (1) patterning neurons to form an arbitrary pattern, (2) patterning neurons onto the electrodes of a standard MEA, and (3) patterning and aligning adult cardiomyocytes in a polystyrene Petri dish.
authors.affiliation: Department of Bioengineering, COMSET, Clemson University, South Carolina 29634, USA.
authors.forename: Russell K
authors.initials: RK
authors.lastname: Pirlo
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ma
authors.forename: Andrew
authors.initials: A
authors.lastname: Sweeney
authors.forename: Honghai
authors.initials: H
authors.lastname: Liu
authors.forename: Julie X
authors.initials: JX
authors.lastname: Yun
authors.forename: Xiang
authors.initials: X
authors.lastname: Peng
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Yuan
authors.forename: George X
authors.initials: GX
authors.lastname: Guo
authors.forename: Bruce Z
authors.initials: BZ
authors.lastname: Gao
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D048430:Cell Shape; D048429:Cell Size; D004566:Electrodes; D007834:Lasers; D032383:Myocytes, Cardiac / Q000166:cytology; D009474:Neurons / Q000166:cytology; D051381:Rats