title: Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif.
abstract: Immune phage antibody libraries are an attractive technology for isolating antigen-specific monoclonal antibodies (mAbs). Here we show that the immunization schedule affects the immune phage antibody library properties. We subcutaneously (s.c.) administered HIV-1 Nef and Vif antigens with different schedules (25 μg × 2 s.c. and 10 μg × 3 s.c.). The variety of isolated mAbs in 25 μg × 2 s.c. groups (Nef: 11 clones, Vif: 9 clones) was superior to that in the 10 μg × 3 s.c. groups (Nef: 2 clones, Vif: 1 clone). This finding suggests that it is important to optimize the immunization schedule for isolating a wide variety of mAbs.
authors.affiliation: Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Yohei
authors.initials: Y
authors.lastname: Mukai
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Tsutsumi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Naoki
authors.initials: N
authors.lastname: Okada
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D003001:Cloning, Molecular; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D007115:Immunization Schedule; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000096:biosynthesis / Q000276:immunology; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000276:immunology*; D054320:vif Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D019151:Peptide Library; D057127:Single-Chain Antibodies; D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1; D054320:vif Gene Products, Human Immunodeficiency Virus; C514847:vif protein, Human immunodeficiency virus 1