title: Human beta-defensin-1, -2, and -3 exhibit opposite effects on oral squamous cell carcinoma cell proliferation.
abstract: The objective of this study was to investigate the impact of human beta-defensins (hBDs) on oral squamous cell carcinoma (OSCC) proliferation and hBD expression in vitro. BHY-OSCC cell lines were stimulated with hBD-1, -2, and -3. Proliferation of BHY cells was ascertained and hBD-mRNA expression was evaluated by real-time PCR. Proliferation of BHY cells decreased by 25% in response to hBD-1 stimulation but increased after stimulation with hBD-2 and -3. HBD-1 stimulation enhanced hBD-3 expression, whereas HBD-2 stimulation decreased early hBD-3 expression. HBD-3 stimulation enhanced hBD-1 expression. HBDs profoundly impact on OSCC proliferation and hBD expression in vitro. Therefore, hBD-1 might function as a tumor suppressor gene in OSCCs, while hBD-2 and -3 might be protooncogenes.
authors.affiliation: Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Winter
authors.forename: Annette
authors.initials: A
authors.lastname: Pantelis
authors.forename: Rudolf
authors.initials: R
authors.lastname: Reich
authors.forename: Markus
authors.initials: M
authors.lastname: Martini
authors.forename: Dominik
authors.initials: D
authors.lastname: Kraus
authors.forename: Soeren
authors.initials: S
authors.lastname: Jepsen
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Allam
authors.forename: Natalija
authors.initials: N
authors.lastname: Novak
authors.forename: Matthias
authors.initials: M
authors.lastname: Wenghoefer
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D012333:RNA, Messenger; D023083:beta-Defensins