title: Biological surface modification of titanium surfaces using glow discharge plasma.
abstract: To improve the biological activity of titanium, by using of glow discharge plasma (GDP), albumin-grafted titanium disk have been implemented and carefully studied. Titanium disks were pre-treated with GDP in an environment filled with argon and allylamine gas. Glutaraldehyde was used as a cross-linking agent for albumin grafting. Then, the surface of the albumin-grafted titanium was examined using scanning electron microscopy and X-ray photoelectron spectroscopy. In addition, the static water contact angles of the albumin-grafted titanium disks were measured using goniometry. To observe the effects of albumin adsorption on cell behavior, MG-63 osteoblast-like cells were cultured on the surface-modified titanium disks. Blood coagulation resistance of the modified titanium was monitored and compared to the control titanium disks. The results demonstrated that MG-63 osteoblast-like cells cultured on the albumin-grafted titanium disks expressed better-differentiated morphology compare to cells grown on the control disks. Furthermore, albumin-grafting treatment significantly improved the surface wettability of the titanium disks and resulted in a significantly negative effect on thrombus formation. Based on these results, it was believed that the GDP can potentially improve the biofunctionality of titanium surfaces.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Haw-Ming
authors.initials: HM
authors.lastname: Huang
authors.forename: Sung-Chih
authors.initials: SC
authors.lastname: Hsieh
authors.forename: Nai-Chia
authors.initials: NC
authors.lastname: Teng
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Feng
authors.forename: Ken-Liang
authors.initials: KL
authors.lastname: Ou
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Chang
mesh_terms: D002454:Cell Differentiation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing / Q000379:methods; D010006:Osteoblasts / Q000166:cytology; D019736:Prostheses and Implants; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D012709:Serum Albumin; D014025:Titanium