title: Cyclin D1 positive diffuse large B-cell lymphoma is a post-germinal center-type lymphoma without alterations in the CCND1 gene locus.
abstract: The aims of this study were to analyze the incidence and morphology of cyclin D1+ DLBCL and cases of Richter transformation (RT), and to elucidate possible molecular mechanisms of cyclin D1 overexpression. Seventy-two cases of de novo DLBCL and 12 cases of RT were included in this study. Cyclin D1 positivity was found in 10/66 (15%) cases of unselected de novo DLBCL and in 2/11 (18%) cases of RT. Seven independently identified cases of cyclin D1+ DLBCL, including one RT, were added to the study. Centroblastic morphology was found in 17/19 (89%) cases of cyclin D1+, most with a post-germinal center phenotype (CD10-, BCL6+, MUM1+). No alterations in the CCND1 gene indicative for a translocation t(11;14) were identified by FISH. Analysis of the MYC locus yielded gene copy alterations in five cases and no disruption of the gene locus in any case, suggesting an alternative mechanism of cyclin D1 deregulation.
authors.affiliation: Institute of Pathology, University of Tübingen, Comprehensive Cancer Center, Tübingen, Germany.
authors.forename: Teresa
authors.initials: T
authors.lastname: Vela-Chávez
authors.forename: Patrick
authors.initials: P
authors.lastname: Adam
authors.forename: Marcus
authors.initials: M
authors.lastname: Kremer
authors.forename: Karin
authors.initials: K
authors.lastname: Bink
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Bacon
authors.forename: Geetha
authors.initials: G
authors.lastname: Menon
authors.forename: Judith A
authors.initials: JA
authors.lastname: Ferry
authors.forename: Falko
authors.initials: F
authors.lastname: Fend
authors.forename: Elaine S
authors.initials: ES
authors.lastname: Jaffe
authors.forename: Leticia
authors.initials: L
authors.lastname: Quintanilla-Martínez
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism*; D020732:Cytogenetic Analysis; D018628:Gene Dosage; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D056426:Genetic Loci / Q000235:genetics; D018858:Germinal Center / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D016393:Lymphoma, B-Cell / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000145:classification* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014178:Translocation, Genetic
chemical_list: D019938:Cyclin D1