title: Noggin and noggin-like genes control dorsoventral axis regeneration in planarians.
abstract: Planarians regenerate a whole animal from a small body piece within a few days. Recent studies have shown that the bone morphogenetic protein (BMP) pathway is required to reestablish the dorsoventral (DV) axis. In vertebrates, the specification of the DV axis depends on the coordinated action of a dual organizer defined by BMP and antidorsalizing morphogenetic protein (ADMP) under the control of several factors, including the inhibitors chordin and noggin. Planarians have an expanded noggin family (up to ten members), which have been classified as canonical noggin (nog) and noggin-like (nlg) genes, the latter carrying an insertion within the noggin domain. Here we show that a BMP/ADMP organizer governs DV axis reestablishment during planarian regeneration, highlighting a greater-than-thought conservation of the mechanisms that establish this axis in protostomes and deuterostomes. Also, we report that whereas noggin genes function as canonical BMP inhibitors, the silencing of planarian nlg8 induces ectopic neurogenesis and enhances ventralizing bmp(RNAi) phenotypes. Finally, we show that noggin-like genes are conserved from cnidarian to vertebrates and that both planarian nlg8 and Xenopus nlg ventralize Xenopus embryos when overexpressed. Remarkably, this ventralization is not associated with an increase in SMAD1/5/8 phosphorylation.
authors.affiliation: Departament de Genètica, Facultat de Biologia, Universitat de Barcelona and Institut de Biomedicina de Universitat de Barcelona, Avinguda Diagonal 645, Edifici Annex, Planta 1, 08028 Barcelona, Catalunya, Spain.
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Molina
authors.forename: Ana
authors.initials: A
authors.lastname: Neto
authors.forename: Ignacio
authors.initials: I
authors.lastname: Maeso
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gómez-Skarmeta
authors.forename: Emili
authors.initials: E
authors.lastname: Saló
authors.forename: Francesc
authors.initials: F
authors.lastname: Cebrià
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D005786:Gene Expression Regulation / Q000502:physiology*; D010802:Phylogeny; D010932:Planarians / Q000502:physiology* / Q000648:ultrastructure; D012038:Regeneration / Q000502:physiology*; D015398:Signal Transduction; D014981:Xenopus / Q000196:embryology; D000099176:Noggin Protein
chemical_list: D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D000099176:Noggin Protein