title: Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast.
abstract: Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96.3% and 84.5% accurate species level identifications (spectral scores, ≥ 1.8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5.1 min of hands-on time/identification), and cost-effective ($0.50/sample) for yeast identification in the clinical laboratory.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Neelam
authors.initials: N
authors.lastname: Dhiman
authors.forename: Leslie
authors.initials: L
authors.lastname: Hall
authors.forename: Sherri L
authors.initials: SL
authors.lastname: Wohlfiel
authors.forename: Seanne P
authors.initials: SP
authors.lastname: Buckwalter
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Wengenack
mesh_terms: D019411:Clinical Laboratory Techniques / Q000191:economics / Q000379:methods*; D003365:Costs and Cost Analysis; D006801:Humans; D009172:Mycology / Q000191:economics / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*