title: Proteomic characterization of Jurkat T leukemic cells after dopamine stimulation: A model of circulating dopamine-sensitive cells.
abstract: T-cells are circulating dopamine-sensitive cells and may mirror, at the peripheral level, biochemical modifications occurring in dopaminergic neurons. The human CD4+ T leukemic Jurkat cell line has been thoroughly used and characterized as a suitable cell model to investigate T-cell signaling and apoptosis. Here, we describe their characterization as a model of circulating dopamine-sensitive cells and their response to a dopamine challenge by analyzing changes in the cell proteome. Jurkat cells express both D1- and D2-like dopamine receptors and both membrane and vesicular transporters, while they lack D4 receptor and tyrosine hydroxylase expression. A saturating, non-toxic, non-oxidant 50 μM dopamine challenge induces the upregulation of an interacting chaperone network known to protect specifically dopaminergic neurons, thus validating T-cells as a biomarker discovery source in Parkinson's disease. Remodeling of the distribution pattern of β-actin and 14-3-3 isoforms is consistently observed upon dopamine treatment. As a whole, dopamine-specific alterations here observed might represent a biosensor for dopamine response at the peripheral level.
authors.affiliation: Department of Structural and Functional Biology, Centre of Neuroscience, University of Insubria, via Alberto da Giussano 12, 21052 Busto Arsizio, Italy. tiziana.alberio@uninsubria.it
authors.forename: Tiziana
authors.initials: T
authors.lastname: Alberio
authors.forename: Claudia
authors.initials: C
authors.lastname: Anchieri
authors.forename: Luca
authors.initials: L
authors.lastname: Piacentini
authors.forename: Giovanna
authors.initials: G
authors.lastname: Gentile
authors.forename: Maurizio
authors.initials: M
authors.lastname: Simmaco
authors.forename: Mara
authors.initials: M
authors.lastname: Biasin
authors.forename: Mauro
authors.initials: M
authors.lastname: Fasano
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D019169:Jurkat Cells; D014176:Protein Biosynthesis; D020033:Protein Isoforms / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; D000199:Actins; D020033:Protein Isoforms; D020543:Proteome; D004298:Dopamine