title: Effect of methyl parathion on nitrous oxide production: a laboratory study.
abstract: We investigated the diversity of a denitrifying gene (nirK) and the emission of CO(2) and N(2)O, in a "chinampa" soil contaminated with methyl parathion. Soil at 40% of water holding capacity was spiked with methyl parathion at four concentrations (i.e. 0, 0.7, 1.47 and 4.27 g kg(-1) dry soil), while emission of N(2)O and CO(2) and nirK diversity was determined after 0, 1, 14, 30, 60 and 90 days. The emission of N(2)O on a daily base and the cumulative emission of CO(2) was not affected by the different concentrations of methyl parathion applied to soil. The diversity of the nirK gene, determined by using temperature gradient gel electrophoresis (TGGE), decreased with increased methyl parathion application. It was found that methyl parathion had effect on the emissions of N(2)O and CO(2), and reduced the diversity of the nirK gene. Consequently, the reduced diversity of the nirK gene could affect the emission of N(2)O.
authors.affiliation: Facultad de Ciencias, Departamento de Ecología y Recursos Naturales, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México D.F., Mexico.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Rojas-Oropeza
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Fernández
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cabirol
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D058440:Denitrification; D004586:Electrophoresis / Q000379:methods; D005798:Genes, Bacterial; D007306:Insecticides / Q000378:metabolism*; D008743:Methyl Parathion / Q000378:metabolism*; D008800:Mexico; D008969:Molecular Sequence Data; D009609:Nitrous Oxide / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D007306:Insecticides; D012989:Soil Pollutants; D002245:Carbon Dioxide; D008743:Methyl Parathion; D009609:Nitrous Oxide