title: New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry.
abstract: This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98°C for 15min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI-TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC-MS/MS using an Agilent 6520 Accurate-Mass Q-TOF.
authors.affiliation: M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation. rustam.ziganshin@gmail.com
authors.forename: Rustam
authors.initials: R
authors.lastname: Ziganshin
authors.forename: Georgii
authors.initials: G
authors.lastname: Arapidi
authors.forename: Igor
authors.initials: I
authors.lastname: Azarkin
authors.forename: Elena
authors.initials: E
authors.lastname: Zaryadieva
authors.forename: Dmitry
authors.initials: D
authors.lastname: Alexeev
authors.forename: Vadim
authors.initials: V
authors.lastname: Govorun
authors.forename: Vadim
authors.initials: V
authors.lastname: Ivanov
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001798:Blood Proteins; D010455:Peptides