title: Thermophoretic melting curves quantify the conformation and stability of RNA and DNA.
abstract: Measuring parameters such as stability and conformation of biomolecules, especially of nucleic acids, is important in the field of biology, medical diagnostics and biotechnology. We present a thermophoretic method to analyse the conformation and thermal stability of nucleic acids. It relies on the directed movement of molecules in a temperature gradient that depends on surface characteristics of the molecule, such as size, charge and hydrophobicity. By measuring thermophoresis of nucleic acids over temperature, we find clear melting transitions and resolve intermediate conformational states. These intermediate states are indicated by an additional peak in the thermophoretic signal preceding most melting transitions. We analysed single nucleotide polymorphisms, DNA modifications, conformational states of DNA hairpins and microRNA duplexes. The method is validated successfully against calculated melting temperatures and UV absorbance measurements. Interestingly, the methylation of DNA is detected by the thermophoretic amplitude even if it does not affect the melting temperature. In the described setup, thermophoresis is measured all-optical in a simple setup using a reproducible capillary format with only 250 nl probe consumption. The thermophoretic analysis of nucleic acids shows the technique's versatility for the investigation of nucleic acids relevant in cellular processes like RNA interference or gene silencing.
authors.affiliation: Physics Department and Center for NanoScience, Ludwig-Maximilians-Universität München, Amalienstrasse 54, 80799 München, Germany.
authors.forename: Christoph J
authors.initials: CJ
authors.lastname: Wienken
authors.forename: Philipp
authors.initials: P
authors.lastname: Baaske
authors.forename: Stefan
authors.initials: S
authors.lastname: Duhr
authors.forename: Dieter
authors.initials: D
authors.lastname: Braun
mesh_terms: D004247:DNA / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D009038:Motion; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D013696:Temperature
chemical_list: D012313:RNA; D004247:DNA