title: Cell therapy for salivary gland regeneration.
abstract: There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like cells in vitro. Therefore, we hypothesized that transplantation with bone marrow stem cells or acinar-like cells may help functional regeneration of salivary glands. Bone marrow stem cells were labeled with nanoparticles and directly co-cultured with acinar cells to obtain labeled acinar-like cells. In total, 140 severely combined immune-deficiency mice were divided into 4 groups for cell therapy experiments: (1) normal mice, (2) mice receiving irradiation around their head-and-neck areas; (3) mice receiving irradiation and intra-gland transplantation with labeled stem cells; and (4) mice receiving irradiation and intra-gland transplantation with labeled acinar-like cells. Our results showed that salivary glands damaged due to irradiation can be rescued by cell therapy with either bone marrow stem cells or acinar-like cells for recovery of saliva production, body weight, and gland weight. Transdifferentiation of bone marrow stem cells into acinar-like cells in vivo was also noted. This study demonstrated that cell therapy with bone marrow stem cells or acinar-like cells can help functional regeneration of salivary glands, and that acinar-like cells showed better therapeutic potentials than those of bone marrow stem cells.
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Lin
authors.forename: F-H
authors.initials: FH
authors.lastname: Chang
authors.forename: C-Y
authors.initials: CY
authors.lastname: Chen
authors.forename: C-Y
authors.initials: CY
authors.lastname: Huang
authors.forename: F-C
authors.initials: FC
authors.lastname: Hu
authors.forename: W-K
authors.initials: WK
authors.lastname: Huang
authors.forename: S-S
authors.initials: SS
authors.lastname: Ju
authors.forename: M-H
authors.initials: MH
authors.lastname: Chen
mesh_terms: D000681:Amylases / Q000096:biosynthesis; D000818:Animals; D016026:Bone Marrow Transplantation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D018920:Coculture Techniques; D016371:Cranial Irradiation / Q000009:adverse effects; D004847:Epithelial Cells / Q000637:transplantation; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011833:Radiation Injuries, Experimental / Q000628:therapy; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000166:cytology / Q000528:radiation effects* / Q000637:transplantation; D014987:Xerostomia / Q000209:etiology / Q000628:therapy*
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D000681:Amylases