title: Cis-cinnamic acid-enhanced 1 gene plays a role in regulation of Arabidopsis bolting.
abstract: Cis-cinnamic acid (CA) is one of many cis-phenylpropanoids found in both monocots and dicots. It is produced in planta via sunlight-mediated isomerization of trans-cinnamic acid. This pair of isomers plays a differential role in regulation of plant growth. A functional proteomics approach has been adopted to identify genes of cis/trans-CA mixture-enhanced expression. Out of 1,241 proteins identified by mass spectrometry, 32 were CA-enhanced and 13 repressed. Further analysis with the molecular biology approach revealed 2 cis-CA (Z usammen-CA)-E nhanced genes, named ZCE1 and ZCE2, which encode members of the major latex protein-like (MLPL) gene family. The transcript accumulation of both genes is positively correlated with the amount of cis-CA applied externally, ranging from 1 to 100 μM. ZCE1 transcript accumulation is enhanced largely by cis-CA and slightly by other cis-phenylpropanoids. Treatment of several well-characterized plant growth regulator perception-deficient mutants with cis-CA is able to promote ZCE1 transcript accumulation, suggestive of distinct signaling pathways regulating cis-CA response. The zce1 loss-of-function mutant produced via the RNA-interference technique produces an earlier bolting phenotype in Arabidopsis, suggesting that ZCE1 plays a role in promoting vegetative growth and delay flowering.
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology, Clear water bay, Hong Kong SAR, China.
authors.forename: Di
authors.initials: D
authors.lastname: Guo
authors.forename: Wai Shing
authors.initials: WS
authors.lastname: Wong
authors.forename: Wen Zhao
authors.initials: WZ
authors.lastname: Xu
authors.forename: Fei Fei
authors.initials: FF
authors.lastname: Sun
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Qing
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002934:Cinnamates / Q000378:metabolism / Q000494:pharmacology; D018744:DNA, Plant / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects; D009154:Mutation; D010802:Phylogeny; D030821:Plants, Genetically Modified; D040901:Proteomics; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D002934:Cinnamates; D018744:DNA, Plant; C029010:cinnamic acid