title: Strain classification of Mycobacterium tuberculosis: congruence between large sequence polymorphisms and spoligotypes.
abstract: Spoligotyping is used in molecular epidemiological studies, and signature patterns have identified strain families. However, homoplasy occurs in the markers used for spoligotyping, which could lead to identical spoligotypes in phylogenetically unrelated strains. We determined the accuracy of strain classification based on spoligotyping using the six large sequence and single nucleotide polymorphisms-defined lineages as a gold standard. Of 919 Mycobacterium tuberculosis isolates, 870 (95%) were classified into a spoligotype family. Strains from a particular spoligotype family belonged to the same lineage. We did not find convergence to the same spoligotype. Spoligotype families appear to be sub-lineages within the main lineages.
authors.affiliation: Francis J Curry National Tuberculosis Center, Division of Pulmonary and Critical Care Medicine, San Francisco General Hospital, University of California San Francisco, California 94110, USA. midori.kato-maeda@ucsf.edu
authors.forename: M
authors.initials: M
authors.lastname: Kato-Maeda
authors.forename: S
authors.initials: S
authors.lastname: Gagneux
authors.forename: L L
authors.initials: LL
authors.lastname: Flores
authors.forename: E Y
authors.initials: EY
authors.lastname: Kim
authors.forename: P M
authors.initials: PM
authors.lastname: Small
authors.forename: E P
authors.initials: EP
authors.lastname: Desmond
authors.forename: P C
authors.initials: PC
authors.lastname: Hopewell
mesh_terms: D015373:Bacterial Typing Techniques; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D012495:San Francisco; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology