title: Combining culture techniques for Bartonella: the best of both worlds.
abstract: In this study we compared some common Bartonella culturing methodologies using four diverse species causing human illnesses. Based on a review of the literature, we focused on three major inconsistencies between protocols: base medium, cell coculture, and temperature. Our data showed that Bartonella tamiae demonstrated temperature-dependent growth limitations between common culturing conditions only 2°C apart. Additionally, growth of B. quintana was significantly enhanced by the presence of mammalian cell coculture under mammalian cell culture conditions; however, when the medium was modified to incorporate insect cell culture-based medium, coculturing with mammalian cells was no longer needed. In this study, we were able to overcome these temperature- and cell-dependent limitations and accommodate all of the strains tested by combining mammalian cell culture-based medium with insect cell culture-based medium.
authors.affiliation: Centers for Disease Control and Prevention, Division of Vector Borne Diseases, 3150 Rampart Road, Fort Collins, CO 80521, USA.
authors.forename: Tarah
authors.initials: T
authors.lastname: Lynch
authors.forename: Jennifer
authors.initials: J
authors.lastname: Iverson
authors.forename: Michael
authors.initials: M
authors.lastname: Kosoy
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D001473:Bartonella / Q000254:growth & development / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000175:diagnosis* / Q000382:microbiology; D018929:Cell Culture Techniques; D002460:Cell Line; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D007313:Insecta; D013696:Temperature
chemical_list: D003470:Culture Media