title: [Effect of Bushenhuoxue Yin on cerebral levels of nitric oxide, tumor necrosis factor-α and interferon-γ in a mouse model of Parkinson disease].
abstract: OBJECTIVE
  To observe the effects of Bushenhuoxue Yin (BSHXY), a compound traditional Chinese herbal medicine, on nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) levels in the brain of a mouse model of Parkinson disease (PD).
  
  
  METHODS
  Forty-five C57BL/6 mice were randomized into MPTP-induced PD model group, BSHXY treatment group and normal control group. The contents of NO and the two cytokines in the brain tissue of the mice were determined by spectroscopy and enzyme-linked immunosorbent assay, respectively.
  
  
  RESULTS
  The concentration of NO in the brain tissue of the PD model group was 5.93 ∓ 0.79 µmol/g.protein, significantly higher than that in BSHXY group (P<0.01) and normal control group (P<0.01). The levels of TNF-α and IFN-γ in the PD model group were 0.36 ∓ 0.11 ng/L and 0.83 ∓ 0.25 ng/L, respectively, also higher than those in the other two groups (P<0.01 or 0.001). But BSHXY group and the normal control group showed no significant differences in the levels of NO, TNF-α and IFN-γ (P>0.05).
  
  
  CONCLUSIONS
  The therapeutic effect of BSHXY on PD is partially attributed to decreased content of NO, TNF-α and IFN-γ in the brain.
authors.affiliation: Institute of Traditional Chinese Medicine, General Hospital of PLA, Beijing 100853, China. lsd301@126.com
authors.forename: Shao-dan
authors.initials: SD
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Ming-hui
authors.initials: MH
authors.lastname: Yang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism*; D010300:Parkinson Disease / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D011897:Random Allocation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; C527851:bushen huoxue; D009569:Nitric Oxide; D007371:Interferon-gamma