title: [The suppression of c-fos in neuron apoptosis induced by heroin].
abstract: OBJECTIVE
  To explore the changes of c-fos in apoptosis of cerebellar granular neuron of neonatal SD rats induced by heroin and the mechanisms of neuronal injury caused by heroin.
  
  
  METHODS
  Primary cerebellar granular neuron were cultured in vitro, the model of apoptosis induced by heroin was established. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were adopted to investigate the changes of c-fos in cell models.
  
  
  RESULTS
  Ten microg/mL of heroin was the optimal dose to induce the apoptosis of cerebellar granular neuron at 48 h. Both Western blotting and RT-PCR showed down regulation of c-fos expression.
  
  
  CONCLUSIONS
  Heroin could induce apoptosis of cerebellar granular neuron and down regulation of c-fos, which may be one of the apoptosis mechanisms.
authors.affiliation: Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. lupeiyan_2001@tom.com
authors.forename: Pei-Yan
authors.initials: PY
authors.lastname: Lu
authors.forename: Ze-Hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Hua-Lan
authors.initials: HL
authors.lastname: Jing
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Lin
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Liu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000187:drug effects; D003932:Heroin / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D003932:Heroin