title: Proteomic analysis of known and candidate rice allergens between non-transgenic and transgenic plants.
abstract: Salt-soluble proteins extracted from non-transgenic and transgenic rice were evaluated for the presence of known and potential allergens by proteomic techniques. The salt-soluble proteins were extracted, separated by 1D and 2D electrophoresis, and analyzed by Western blotting. 1D immunoblot analysis with patients' sera revealed few qualitative differences between the IgE-binding proteins of the non-transgenic and transgenic rice. 1D immunoblot with antigen-specific-animal sera revealed no qualitative or quantitative differences in two known allergens, RAG2 and glyoxalase I, between non-transgenic and transgenic rice. Multiple spots containing known and novel IgE-binding proteins were detected among the salt-soluble proteins of non-transgenic rice by 2D immunoblotting. Two globulin-like proteins, a 52 kDa protein and a 63 kDa protein, were identified as novel IgE-binding proteins that are candidates for rice allergens. These globulin-like proteins were homologous to Cupin superfamily allergens. Quantitative analysis of 19, 52, and 63 kDa globulins with protein-specific-animal sera showed no significant differences in the expression of these proteins between the transgenic rice and non-transgenic rice. These results indicate that none of the known or novel endogenous IgE-binding proteins detected in this study appear to be altered by genetic modification.
authors.affiliation: Division of Novel Foods and Immunochemistry, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Satoh
authors.forename: Rika
authors.initials: R
authors.lastname: Nakamura
authors.forename: Akira
authors.initials: A
authors.lastname: Komatsu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Oshima
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D001666:Binding Sites, Antibody; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000276:immunology*; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology*; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000235:genetics / Q000276:immunology
chemical_list: D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E