title: A novel chemiluminescence quenching method for determination of sulfonamides in pharmaceutical and biological fluid based on luminol-Ag(III) complex reaction in alkaline solution.
abstract: A novel chemiluminescence (CL) quenching method for the determination of sulfonamides is proposed. The CL reaction between Ag(III) complex [Ag(HIO₆)₂]⁵⁻ and luminol in alkaline solution was investigated. The quenching effect of sulfonamides on CL emission of [Ag(HIO₆)₂]⁵⁻-luminol system was found. Quenching degree of CL emission was proportional to sulfonamide concentration. The effects of the reaction conditions on CL emission and quenching were examined. Under optimal conditions, the detection limits (s/n = 3) were 7.2, 17 and 8.3 ng/mL for sulfadiazine, sulfameter, and sulfadimethoxine, respectively. The recoveries of the three drugs were in the range of 91.3-110% with RSDs of 1.9-2.7% for urine samples, and 106-112% with RSDs of 1.6-2.8% for serum samples. The proposed method was used for the determination of sulfadiazine at clinically relevant concentrations in real urine and serum samples with satisfactory results.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China. hanwen@hbu.edu.cn
authors.forename: Hanwen
authors.initials: H
authors.lastname: Sun
authors.forename: Peiyun
authors.initials: P
authors.lastname: Chen
authors.forename: Liqing
authors.initials: L
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013411:Sulfadiazine / Q000032:analysis* / Q000097:blood / Q000652:urine; D013412:Sulfadimethoxine / Q000032:analysis* / Q000097:blood / Q000652:urine; D013417:Sulfameter / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D000890:Anti-Infective Agents; D013411:Sulfadiazine; D013412:Sulfadimethoxine; D013417:Sulfameter; D012834:Silver; D008165:Luminol