title: [Animal models for bone and joint disease. Bone disease of osteoprotegerin deficient mouse].
abstract: Osteoprotegerin (OPG) acts as a decoy receptor for the receptor activator of the nuclear factor κ B (RANK) ligand (RANKL) , preventing its association with RANK and inhibiting osteoclastogenesis. Therefore, mice homozygous for targeted disruption of the OPG gene reveal stimulated bone resorption and bone formation, resulting in enhanced bone remodeling. The OPG deficient (OPG( - / - )) mouse showed the diturbed distribution of collagen fibers and complex meshwork of cement lines, which implies weakened strength of OPG( - / - ) bone against mechanical stress. In addition, the abnormally promoted remodeling of the OPG( - / - ) bone caused the disorganized distribution of osteocyte lacunar canalicular system (OLCS) . Histochemical assessment revealed the markedly reduced synthesis of sclerostin in the OPG( - / - ) OLCS while the synthesis of dentin matrix protein-1 was not extremely affected by the OPG deficiency. Taken together, OPG deficient mouse appears to be a valid model for extremely-stimulated bone remodeling, and would provided important clues for better understanding for activities of bone cells in a pathological state in bone.
authors.affiliation: Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University.
authors.forename: Tomoka
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Muneteru
authors.initials: M
authors.lastname: Sasaki
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tabata
authors.forename: Hideo
authors.initials: H
authors.lastname: Masuki
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016723:Bone Remodeling; D001862:Bone Resorption; D001842:Bone and Bones / Q000378:metabolism; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000502:physiology; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D010006:Osteoblasts / Q000502:physiology; D010010:Osteoclasts / Q000502:physiology; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D053244:Osteoprotegerin / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D053245:RANK Ligand
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D053244:Osteoprotegerin; D053245:RANK Ligand; C486030:Sost protein, mouse; D053495:Osteopontin; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase