title: Caryophyllene oxide-rich essential oils of Lithuanian Artemisia campestris ssp. campestris and their toxicity.
abstract: The chemical composition of the essential oils of aerial parts of Artemisia campestris ssp. campestris, collected from ten different locations in Lithuania is detailed in this paper. The major component in all the oils was caryophyllene oxide (8.5-38.8%), whereas compounds with the caryophyllane skeleton ranged from 10.2 to 44.5%. Other representative constituents were germacrene D (< or = 15.0%), humulene epoxide II (< or = 8.1%), beta-ylangene (< or = 7.7%), spathulenol (< or = 6.8%), beta-elemene (< or = 6.8%), beta-caryophyllene (< or = 6.2%), junenol (< or = 6.1%) and alpha- or beta-pinene (< or = 5.5%). Eighty-seven compounds were identified, comprising 73.6-92.3% of the oils. The chemical composition was highly variable depending on the sample location. Toxicity of A. campestris oils was determined using the brine shrimp (Artemia sp.) assay. LC50 values ranging to 20 microg/mL were obtained for three of the oils after 24 hours of exposure. Data of this test revealed that A. campestris ssp. campestris essential oils with dominant caryophyllene oxide are notably toxic.
authors.affiliation: Center for Physical Sciences and Technology, Institute of Chemistry, A. Gostauto 9, LT-01108 Vilnius, Lithuania. judzent@ktl.mii.lt
authors.forename: Asta
authors.initials: A
authors.lastname: Judzentiene
authors.forename: Jurga
authors.initials: J
authors.lastname: Budiene
authors.forename: Rita
authors.initials: R
authors.lastname: Butkiene
authors.forename: Eugenija
authors.initials: E
authors.lastname: Kupcinskiene
authors.forename: Isabelle
authors.initials: I
authors.lastname: Laffont-Schwob
authors.forename: Véronique
authors.initials: V
authors.lastname: Masotti
mesh_terms: D000818:Animals; D001156:Artemia; D019306:Artemisia / Q000737:chemistry*; D009822:Oils, Volatile / Q000032:analysis* / Q000633:toxicity; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000032:analysis*
chemical_list: D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C515179:caryophyllene oxide