title: [Comparison of viremia formation between guinea-pigs infected with wild and attenuated (SA14-14-2) Japanese encephalitis viruses].
abstract: OBJECTIVE
  To study the viremia formation in guinea-pigs infected with wild type and attenuated Japanese encephalitis virus (JEV).
  
  
  METHODS
  Guniea pigs were inoculated intraperitoneally with different wild JEV strains and the attenuated vaccine strain and its parent virulent strain. Viremia was detected on different days following virus inoculation.
  
  
  RESULTS
  All the guinea-pigs inoculated with the wild JEV strains induced different levels of viremia (1.00-3.40 Lg pfu) on the 1st and 3rd day post inoculation. Using a virus titer of 10(4) pfu for inoculation, the animals inoculated with the SA14 parent strain induced relatively high viremia (10(2.4)-10(3.4) pfu), however no viremia coulds be detected on any tested days.
  
  
  CONCLUSIONS
  The degree of viremia in guinea pigs can be used as a new method to evaluate the attenuation of JEV.
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, China.
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Liu
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Yu
authors.forename: Hong-shan
authors.initials: HS
authors.lastname: Xu
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liang
authors.forename: Huan-yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Li-li
authors.initials: LL
authors.lastname: Jia
authors.forename: Guan-mu
authors.initials: GM
authors.lastname: Dong
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004664:Encephalitis Virus, Japanese / Q000472:pathogenicity* / Q000502:physiology; D004672:Encephalitis, Japanese / Q000821:virology*; D006168:Guinea Pigs; D006801:Humans; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000009:adverse effects; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects; D014766:Viremia / Q000821:virology*; D014774:Virulence; D014779:Virus Replication
chemical_list: D022321:Japanese Encephalitis Vaccines; D014613:Vaccines, Attenuated