title: Anticancer and antimetastatic activities of Renieramycin M, a marine tetrahydroisoquinoline alkaloid, in human non-small cell lung cancer cells.
abstract: BACKGROUND
  Renieramycin M, has been shown to exhibit promising anticancer activity against some cancer cell lines; however, the underlying mechanism remains unknown.
  
  
  METHODS
  Renieramycin M was isolated from the blue sponge Xestospongia sp. Anticancer and antimetastatic activities of renieramycin M were investigated in human non-small cell lung cancer cells.
  
  
  RESULTS
  Renieramycin M treatment caused p53 activation, which subsequently down-regulated anti-apoptotic MCL-1 and BCL-2 proteins, while the level of pro-apoptotic BAX protein was not altered. The subtoxic concentrations of renieramycin M significantly decreased invasion and migration abilities of cancer cells. In addition, this compound showed a strong inhibitory effect on anchorage-independent growth of the cells.
  
  
  CONCLUSIONS
  These results reveal that renieramycin M induced lung cancer cells apoptosis through p53-dependent pathway and the compound may inhibit progression and metastasis of lung cancer cells.
authors.affiliation: Pharmaceutical Technology (International) Program, Organisms and Endophytic Fungi (BNPME), Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Pathumwan, Bangkok, Thailand.
authors.forename: Hasseri
authors.initials: H
authors.lastname: Halim
authors.forename: Preedakorn
authors.initials: P
authors.lastname: Chunhacha
authors.forename: Khanit
authors.initials: K
authors.lastname: Suwanborirux
authors.forename: Pithi
authors.initials: P
authors.lastname: Chanvorachote
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D023102:Anoikis / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D011161:Porifera / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D044005:Tetrahydroisoquinolines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000470:Alkaloids; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D044005:Tetrahydroisoquinolines; D016159:Tumor Suppressor Protein p53; C482478:renieramycin M