title: The ribonuclease/angiogenin inhibitor is also present in mitochondria and nuclei.
abstract: The data presented here show for the first time that the protein known as "ribonuclease (RNase) inhibitor" (RI or RNH1) is present not only in the cell cytosol, but also in mitochondria, the central organelles in cell redox homeostasis. This finding directly correlates with the reported ability of RI to protect the cell from oxidative stress, with its sensitivity to oxidation and reactivity as a reactive oxygen species scavenger. While this study was carried out we also surprisingly discovered the presence of RI in the cell nucleus. We deem that these data open new views in the investigation on the cellular role(s) of the RI.
authors.affiliation: Department of Structural and Functional Biology, University of Naples Federico II, Naples, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Furia
authors.forename: Marco
authors.initials: M
authors.lastname: Moscato
authors.forename: Gaetano
authors.initials: G
authors.lastname: Calì
authors.forename: Elio
authors.initials: E
authors.lastname: Pizzo
authors.forename: Elena
authors.initials: E
authors.lastname: Confalone
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Amoroso
authors.forename: Franca
authors.initials: F
authors.lastname: Esposito
authors.forename: Lucio
authors.initials: L
authors.lastname: Nitsch
authors.forename: Giuseppe
authors.initials: G
authors.lastname: D'Alessio
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; C501467:RNH1 protein, human; D011993:Recombinant Fusion Proteins