title: Carrefour Mme. Gras: a wild-isolated Neurospora crassa strain that suppresses meiotic silencing by unpaired DNA and uncovers a novel ascospore stability defect.
abstract: Ordinarily, RIP-induced erg-3 mutant Neurospora crassa ascospores and their erg(+) siblings do not differ in stability during long-term storage. Consequently, the frequency of RIP-induced erg-3 mutants remains about constant regardless of the time that has elapsed between ascospore harvest and germination. We found, however, that RIP-induced erg-3 mutants were apparently selectively lost with time from among the ascospores stored from a cross with the wild-isolated Carrefour Mme. Gras strain from Haiti. The Haitian strain was also found to exert a dominant suppression of meiotic silencing by unpaired DNA. Similar loss of RIP-induced erg-3 mutant ascospores was seen among the stored ascospores from a subset of crosses heterozygous for the semi-dominant Sad-1 or Sad-2 suppressors of meiotic silencing. Our results suggest that crosses suppressed in meiotic silencing can compromise the stability during storage of ascospores that inherit RIP-induced mutations.
authors.affiliation: Centre for Cellular and Molecular Biology (CSIR), Hyderabad 500 007, India. kas@ccmb.res.in
authors.forename: Durgadas P
authors.initials: DP
authors.lastname: Kasbekar
authors.forename: Parmit K
authors.initials: PK
authors.lastname: Singh
authors.forename: Mukund
authors.initials: M
authors.lastname: Ramakrishnan
authors.forename: Kranthi B
authors.initials: KB
authors.lastname: Raj
mesh_terms: D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D008540:Meiosis; D009154:Mutation; D009492:Neurospora crassa / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D013172:Spores, Fungal / Q000166:cytology* / Q000235:genetics / Q000254:growth & development
chemical_list: D004271:DNA, Fungal