title: In situ generation of hydrogen peroxide by carbohydrate oxidase and cellobiose dehydrogenase for bleaching purposes.
abstract: The carbohydrate oxidase from Microdochium nivale (CAOX), heterologously expressed in Aspergillus oryzae, and cellobiose dehydrogenase from Myriococcum thermophilum (MtCDH), were assessed for their ability to generate bleaching species at a pH suitable for liquid detergents. The substrate specificities of CAOX and MtCDH were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. Even insoluble substrates like cellulose were oxidized from both CAOX and MtCDH, but only MtCDH produced H₂O₂ on cotton as the sole substrate. To enhance the amount of cello-oligosaccharides formed from cotton as substrates for CAOX and MtCDH, various cellulases were used in combination with MtCDH or CAOX, leading to a 10-fold increase in H₂O₂. As model substrates for colored stains, the degradation of pure anthocyanins and stain removal of blueberry stains by CAOX and MtCDH was examined in the absence and presence of a horseradish peroxidase. Both enzymes were able to produce an amount of H₂O₂ sufficient to decolorize the pure anthocyanins within 2 h and showed significant cleaning benefits on the stains.
authors.affiliation: Department of Environmental Biotechnology, Graz University of Technology, Graz, Austria.
authors.forename: Sina
authors.initials: S
authors.lastname: Pricelius
authors.forename: Roland
authors.initials: R
authors.lastname: Ludwig
authors.forename: Neil J
authors.initials: NJ
authors.lastname: Lant
authors.forename: Dietmar
authors.initials: D
authors.lastname: Haltrich
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D001236:Aspergillus oryzae / Q000378:metabolism; D001487:Basidiomycota / Q000201:enzymology*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods; D010088:Oxidoreductases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003902:Detergents; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D006735:Horseradish Peroxidase