title: Intragastrically administered lysophosphatidic acids protect against gastric ulcer in rats under water-immersion restraint stress.
abstract: OBJECTIVE
  Lysophosphatidic acid exerts important physiological effects on many types of animal cells through its specific binding to several G protein-coupled receptors. In particular, its potent wound-healing effect has attracted much attention. To determine whether lysophosphatidic acids in a foodstuff and Chinese medicine are effective in protecting against gastric ulcer, we subjected rats to water-immersion restraint stress.
  
  
  RESULTS
  Three direct administrations of a solution of lysophosphatidic acid with a C18 fatty acyl group to the rat stomach in a concentration range of 0.001-0.1 mM resulted in a significant reduction in the number of gastric ulcers induced during water-immersion restraint stress, and the potencies were as follows: linoleoyl species=α-linolenoyl species>oleoyl species. Intragastric administrations of a solution of highly purified lysophosphatidic acid from soybean lecithin significantly protected against the stress-induced gastric ulcers at lower concentrations than partially purified lysophosphatidic acid from soybean lecithin did. In addition, administration of a decocted solution of antyu-san, and lysophosphatidic acid-rich Chinese medicine, to the stomach was more effective in protecting against stress-induced ulcer than decoctations of antyu-san lacking the corydalis tuber component that is rich in lysophosphatidic acid.
  
  
  CONCLUSIONS
  These results clearly show that lysophosphatidic acid is the effective component of soybean lecithin and antyu-san in protection against stress-induced gastric ulcer in the rat model, and suggest that daily intake of lysophosphatidic acid-rich foods or Chinese medicines may be beneficial for prevention of stress-induced gastric ulcer in human subjects.
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, Tokushima, 770-8505, Japan. c400842002@stud.tokushima-u.ac.jp
authors.forename: Mika
authors.initials: M
authors.lastname: Adachi
authors.forename: Gou
authors.initials: G
authors.lastname: Horiuchi
authors.forename: Natsuki
authors.initials: N
authors.lastname: Ikematsu
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Junji
authors.initials: J
authors.lastname: Terao
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Satouchi
authors.forename: Akira
authors.initials: A
authors.lastname: Tokumura
mesh_terms: D000818:Animals; D031311:Corydalis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005753:Gastric Mucosa / Q000187:drug effects; D007101:Immersion / Q000009:adverse effects; D054709:Lecithins / Q000008:administration & dosage; D008246:Lysophospholipids / Q000008:administration & dosage* / Q000302:isolation & purification; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical / Q000009:adverse effects; D013025:Glycine max / Q000737:chemistry; D013276:Stomach Ulcer / Q000209:etiology / Q000517:prevention & control*; D013315:Stress, Psychological / Q000150:complications*
chemical_list: D004365:Drugs, Chinese Herbal; D054709:Lecithins; D008246:Lysophospholipids; C032881:lysophosphatidic acid