title: [Integration sites in HCC biopsy].
abstract: OBJECTIVE
  To compare the performance of Inverse-PCR, Alu-PCR and Cassette-ligation-mediated PCR (CLM-PCR) in HBV DNA integration sites identification.
  
  
  METHODS
  One HCC biopsy was obtained from surgically resected sample. The patient was positive for serum hepatitis B surface antigen (HBsAg). The genomic DNA was purified by the standard phenol/chloroform extraction and ethanol precipitation method. Seperated set of primers were designed to amplify the HBV DNA integration region by means of 3 different PCR methods respectively. The PCR products were analyzed by electrophoresis, then cloned to PMD18-T vector for DNA sequencing. The sequence alignment was performed under Blast software.
  
  
  RESULTS
  7 bands and 22 sequencing results was obtained from IPCR and 3 integration sites was identified. Alu-PCR provided 12 bands and 32 sequencing results, and CLM-PCR showed 12 bands and 4 sequencing results. No integration site was identified from the latter two.
  
  
  CONCLUSIONS
  IPCR compared with another two methods showed a reliable capacity in HBV DNA integration site identification.
authors.affiliation: The Surgical Department of The First Clinical College of GuangDong Medical College, ZhanJiang 524023, China.
authors.forename: Lan-tian
authors.initials: LT
authors.lastname: Wang
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Zhu
authors.forename: Bo-ping
authors.initials: BP
authors.lastname: Zhou
authors.forename: Wei-long
authors.initials: WL
authors.lastname: Liu
authors.forename: Ming-feng
authors.initials: MF
authors.lastname: Lano
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Li
authors.forename: Xin-chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Li
mesh_terms: D000328:Adult; D001706:Biopsy; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000821:virology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D016662:Virus Integration