title: Mutation of GOGAT prevents pea bacteroid formation and N2 fixation by globally downregulating transport of organic nitrogen sources.
abstract: Mutation of gltB (encoding glutamate oxoglutarate amidotransferase or GOGAT) in RU2307 increased the intracellular Gln:Glu ratio and inhibited amino acid transport via Aap and Bra. The mechanism probably involves global post-translational inhibition independent of Ntr. Transport was separately restored by increased gene expression of Aap or heterologous transporters. Likewise, second site suppressor mutations in the RNA chaperone Hfq elevated transport by Aap and Bra by increasing mRNA levels. Microarrays showed Hfq regulates 34 ABC transporter genes, including aap, bra and opp. The genes coding for integral membrane proteins and ABC subunits aapQMP braDEFGC were more strongly elevated in the hfq mutants than solute-binding proteins (aapJ braC). aapQMP and braDEFG are immediately downstream of stem-loops, indicating Hfq attenuates downstream translation and stability of mRNA, explaining differential expression of ABC genes. RU2307 nodulated peas and bacteria grew down infection threads, but bacteroid development was arrested and N(2) was not fixed. This probably results from an inability to synthesize or transport amino acids. However, GOGAT and GOGAT/AldA double mutants carrying suppressor mutations that increased amino acid uptake fixed N(2) on pea plants. Thus de novo ammonium assimilation into amino acids is unnecessary in bacteroids demonstrating sufficient amino acids are supplied by plants.
authors.affiliation: Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
authors.forename: G
authors.initials: G
authors.lastname: Mulley
authors.forename: J P
authors.initials: JP
authors.lastname: White
authors.forename: R
authors.initials: R
authors.lastname: Karunakaran
authors.forename: J
authors.initials: J
authors.lastname: Prell
authors.forename: A
authors.initials: A
authors.lastname: Bourdes
authors.forename: S
authors.initials: S
authors.lastname: Bunnewell
authors.forename: L
authors.initials: L
authors.lastname: Hill
authors.forename: P S
authors.initials: PS
authors.lastname: Poole
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation / Q000235:genetics / Q000502:physiology*; D018532:Pisum sativum / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016961:Rhizobium leguminosarum / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D009584:Nitrogen