title: Epicatechin administration leads to necrotic cell death of rat leukaemia promyelocytes in vivo.
abstract: BACKGROUND
  (-)-Epicatechin (EC) induces oxidative DNA damage in HL-60 cells. The association between genotoxic and apoptotic/necrotic effects of EC was studied in rats with acute myeloid leukaemia.
  
  
  METHODS
  Healthy and leukaemic rats were given EC by oral gavage at a dose of 40 mg/kg body weight for 22 consecutive days. Bone marrow cells were subjected to analysis of DNA damage by the comet assay and apoptosis by flow cytometry.
  
  
  RESULTS
  EC significantly increased DNA strand breaks in bone marrow cells of leukaemic animals but it did not exert such an effect on healthy rats. EC action led to necrosis of leukaemia cells but it did not induce apoptosis of these cells in comparison to the controls.
  
  
  CONCLUSIONS
  EC has genotoxic and necrotic effects which may have utility in anticancer therapy against acute myeloid leukaemia.
authors.affiliation: Department of Cytobiology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688 Krakow, Poland. mfpapiez@cyf-kr.edu.pl
authors.forename: Monika A
authors.initials: MA
authors.lastname: Papiez
authors.forename: Jarosław
authors.initials: J
authors.lastname: Baran
authors.forename: Karolina
authors.initials: K
authors.lastname: Bukowska-Straková
authors.forename: Mirosław
authors.initials: M
authors.lastname: Krośniak
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000187:drug effects; D002392:Catechin / Q000008:administration & dosage*; D020552:Comet Assay; D053960:DNA Breaks; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009336:Necrosis; D051381:Rats; D011914:Rats, Inbred BN
chemical_list: D002392:Catechin