title: Comparison of two diode lasers on bactericidity in root canals--an in vitro study.
abstract: This in vitro study compares two 810-nm and 940-nm diode lasers on bacterial kill in root canals of extracted human teeth and shows the clinical relevance of different treatment modalities. Ninety root canals of single-rooted human teeth were prepared up to ISO 70, steam sterilized, and assigned to two test groups (810 nm, 940 nm) and one control group. Following an initiatory experiment in which access opening of root canals and surrounding cavity were excluded from irradiation in the main experiment, 60 teeth were inoculated with 2 μl of either Escherichia coli or Enterococcus faecalis suspension. Laser irradiation was performed, additionally including access opening of root canals and surrounding cavity in the laser treatment. Excluding access opening of root canals and surrounding cavity from the laser treatment, the diode laser achieved an average bacterial reduction of Escherichia coli of 76.06% (810 nm) and 68.15% (940 nm), while including access cavities showed an average bacterial reduction of Escherichia coli of 97.84% (810 nm) and 98.83% (940 nm) and an average bacterial reduction of Enterococcus faecalis of 98.8% (810 nm) and 98.66% (940 nm). Diode laser wavelengths are effective in endodontic therapy. It seems to be clinically relevant that additional irradiation of the access cavity produces significantly better bactericidal results.
authors.affiliation: Bernhard Gottlieb University Dental School, Medical University Vienna, Währingerstrasse 25 a, 1090, Vienna, Austria. franziska.beer@meduniwien.ac.at
authors.forename: Franziska
authors.initials: F
authors.lastname: Beer
authors.forename: Alfred
authors.initials: A
authors.lastname: Buchmair
authors.forename: Johann
authors.initials: J
authors.lastname: Wernisch
authors.forename: Apostolos
authors.initials: A
authors.lastname: Georgopoulos
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001424:Bacterial Infections / Q000188:drug therapy; D058491:Bacterial Load / Q000528:radiation effects; D003786:Dental Pulp Cavity / Q000382:microbiology / Q000528:radiation effects*; D004708:Endodontics; D013293:Enterococcus faecalis / Q000528:radiation effects*; D004926:Escherichia coli / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D014081:Tooth Extraction
chemical_list: D000900:Anti-Bacterial Agents