title: Neutron reflectometry of supported hybrid bilayers with inserted peptide.
abstract: The insertion of a synthetic amphiphilic, α-helical peptide into a supported hybrid bilayer membrane (HBM) was studied by neutron reflectometry to elucidate the resulting nanostructure. The HBM consisted of a self-assembled monolayer of perdeuterated octadecanethiol on gold and an overlying leaflet of acyl-deuterated phosphatidylcholine (d-DMPC). Using contrast variation, several reflectivity spectra were recorded for each step of film fabrication, and simultaneously modeled. This analysis indicated that peptide insertion into the DMPC lipid leaflet is the likeliest mode of incorporation.
authors.affiliation: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Smith
authors.forename: Duncan J
authors.initials: DJ
authors.lastname: McGillivray
authors.forename: Jan
authors.initials: J
authors.lastname: Genzer
authors.forename: Mathias
authors.initials: M
authors.lastname: Lösche
authors.forename: Peter K
authors.initials: PK
authors.lastname: Kilpatrick