title: [Oxidation status and γ-glutamylcysteine synthetase expression in the kidney of MRL/lpr lupus mice].
abstract: OBJECTIVE
  [corrected] To investigate the role of glutathione (GSH) and γ-glutamylcysteine synthetase (γ-GCS) in lupus nephritis.
  
  
  METHODS
  Spectrophotometry was used to measure the oxidative/anti-oxidative indices including malonyldialdehyed (MDA) and GSH in the kidney of MRL/lpr lupus mice. Quantitative PCR and Western blotting were used to detected the expression of γ-GCS.
  
  
  RESULTS
  The level of GSH was lowered whereas the level of MDA increased significantly in the kidney tissue of MRL/lpr lupus mice as compared with that in normal control mice. The expression of γ-GCS mRNA and protein was significantly decreased in MRL/lpr lupus mice (P<0.05).
  
  
  CONCLUSIONS
  MRL/lpr lupus mice have abnormal oxidative stress in the kidney tissue, where the expression of γ-GCS decreased to lead to reduced GSH production, damaged antioxidative capacity, and eventually exacerbation of oxidative damage in the kidney.
authors.affiliation: Department of Rheumatology and Immunology, Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China. ruilinchen@126.com
authors.forename: Rui-lin
authors.initials: RL
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Tao
authors.forename: Bei-ping
authors.initials: BP
authors.lastname: Zhang
authors.forename: Zhi-fen
authors.initials: ZF
authors.lastname: Lv
mesh_terms: D000818:Animals; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D008181:Lupus Nephritis / Q000201:enzymology* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D019463:Mice, Inbred MRL lpr; D018384:Oxidative Stress / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D008315:Malondialdehyde; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione