title: In vitro antiperoxidative, free radical scavenging and xanthine oxidase inhibitory potentials of ethyl acetate fraction of Saraca ashoka flowers.
abstract: Saraca ashoka is a widely used medicinal herb claimed to cure many diseases. This study investigated the antiperoxidative, free radical scavenging and xanthine oxidase (XO) inhibitory potential of the ethyl acetate fraction of S. ashoka flowers (SAF) and compared it with standard compounds like gallic acid, ascorbic acid, butylated hydroxyl toluene and allopurinol. The ethyl acetate fraction of SAF exhibited free radical scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and superoxide radical, along with hydroxyl radical scavenging activity. Lipid peroxidation inhibitory potential of SAF was studied using a linoleic acid emulsion system, which shows significant antioxidant potential. SAF also demonstrated significant XO (key enzyme linked to inflammation) inhibitory activity, which revealed its therapeutic potential as an antioxidant and XO inhibitor. HPLC profiling of the ethyl acetate fraction of SAF revealed that it contains ellagic acid as a major compound and thus the beneficial effects of this fraction may be due to the presence of this compound.
authors.affiliation: Biochemistry and Cell Culture Laboratory, Agro Processing and Natural Products Division, National Institute for Interdisciplinary Science and Technology, Council of Scientific and Industrial Research, Thiruvananthapuram 695019, Kerala, India.
authors.forename: A
authors.initials: A
authors.lastname: Prathapan
authors.forename: O
authors.initials: O
authors.lastname: Lijo Cherian
authors.forename: Suresh V
authors.initials: SV
authors.lastname: Nampoothiri
authors.forename: S
authors.initials: S
authors.lastname: Mini
authors.forename: K G
authors.initials: KG
authors.lastname: Raghu
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D000085:Acetates; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; C007650:ethyl acetate; D014969:Xanthine Oxidase