title: Mitochondrial dysfunction and genetic heterogeneity in chronic periodontitis.
abstract: We performed an extensive study on mitochondrial dysfunction in chronic periodontitis (CP). Electron microscopic analysis of gingival cells revealed abnormal mitochondria in 60% of the patients. Mitochondrial membrane potential and oxygen consumption of gingival cells were reduced by 4 fold and 5.8 fold, respectively; whereas ROS production was increased by 18%. The genetic analysis by complete mitochondrial DNA sequencing revealed the identification of 14 novel mutations only in periodontal tissues but not in the blood, suggesting a role of oxidative stress on periodontal tissues. Thus, our functional and genetic analysis provided an evidence for the mitochondrial dysfunction in CP.
authors.affiliation: Centre for Cellular and Molecular Biology (CSIR), Hyderabad, India.
authors.forename: Periyasamy
authors.initials: P
authors.lastname: Govindaraj
authors.forename: Nahid Akhtar
authors.initials: NA
authors.lastname: Khan
authors.forename: Praturi
authors.initials: P
authors.lastname: Gopalakrishna
authors.forename: Rampalli Viswa
authors.initials: RV
authors.lastname: Chandra
authors.forename: Ayyasamy
authors.initials: A
authors.lastname: Vanniarajan
authors.forename: Aileni Amarendra
authors.initials: AA
authors.lastname: Reddy
authors.forename: Shashi
authors.initials: S
authors.lastname: Singh
authors.forename: Rathinam
authors.initials: R
authors.lastname: Kumaresan
authors.forename: Gunda
authors.initials: G
authors.lastname: Srinivas
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Thangaraj
mesh_terms: D000328:Adult; D055113:Chronic Periodontitis / Q000235:genetics* / Q000473:pathology*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004847:Epithelial Cells / Q000648:ultrastructure*; D005260:Female; D018740:Genetic Heterogeneity; D054629:Genome, Mitochondrial; D005881:Gingiva / Q000473:pathology*; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D010100:Oxygen