title: Proteolysis and bioconversion of cereal proteins to glutamate and γ-Aminobutyrate (GABA) in Rye malt sourdoughs.
abstract: This study aimed to achieve the conversion of cereal proteins to the alternative end products glutamate or γ-aminobutyrate (GABA). Rye malt, fungal proteases, and lactobacilli were employed to convert wheat gluten or barley proteins. Glutamate and GABA formations were strain-dependent. Lactobacillus reuteri TMW1.106 and Lactobacillus rossiae 34J accumulated glutamate; L. reuteri LTH5448 and LTH5795 accumulated GABA. Glutamate and GABA accumulation by L. reuteri TMW1.106 and LTH5448 increased throughout fermentation time over 96 h, respectively. Peptides rather than amino acids were the main products of proteolysis in all doughs, and barley proteins were more resistant to degradation by rye malt proteases than wheat gluten. However, addition of fungal protease resulted in comparable degradation of both substrates. Glutamate and GABA accumulated to concentrations up to 63 and 90 mmol kg(-1) DM, respectively. Glutamate levels obtained through bioconversion of cereal proteins enable the use of hydrolyzed cereal protein as condiment.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta , 410 Ag/For Centre, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Achim
authors.initials: A
authors.lastname: Stromeck
authors.forename: Ying
authors.initials: Y
authors.lastname: Hu
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.forename: Michael G
authors.initials: MG
authors.lastname: Gänzle
mesh_terms: D001939:Bread / Q000382:microbiology; D002523:Edible Grain / Q000737:chemistry* / Q000201:enzymology; D005285:Fermentation; D005658:Fungi / Q000201:enzymology; D018698:Glutamic Acid / Q000378:metabolism*; D005983:Glutens / Q000378:metabolism; D001467:Hordeum / Q000737:chemistry; D007778:Lactobacillus / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D012434:Secale / Q000201:enzymology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005983:Glutens; D010447:Peptide Hydrolases