title: Co-expression of serotonin 5-HT(1B) and 5-HT(4) receptors in p11 containing cells in cerebral cortex, hippocampus, caudate-putamen and cerebellum.
abstract: p11 is an adaptor protein which binds to serotonin 5-HT(1B) receptors and 5-HT(4) receptors and regulates their localization at the cell surface. In the present study, we examined to what extent p11 containing neurons co-expressed 5-HT(1B)R and/or 5-HT(4)R in cerebral cortex, hippocampus, cerebellum and caudate-putamen. A triple-labeling immunohistochemical approach was taken using antibodies to detect native p11 and 5-HT(1B)R combined with visualization of EGFP driven under the 5-HT(4)R promoter in BAC-transgenic mice. In the caudate-putamen, the hippocampal pyramidal cell layer of CA1 and the hippocampal granule cell layer of dentate gyrus, most p11 containing cells co-expressed both 5-HT(1B)R and 5-HT(4)R. In the cingulate cortex, stratum radiatum/oriens of CA1, hilus of the dentate gyrus and cerebellar cortex, many cells co-expressed p11 and 5-HT(1B)R, but not 5-HT(4)R. In the studied brain regions, few cells solely expressed p11 without any significant expression of 5-HT(1B)R or 5-HT(4)R. It can be concluded that p11 is anatomically positioned to modulate serotonin neurotransmission, via 5-HT(1B)R and 5-HT(4)R, in brain regions important for emotionality, cognition and locomotion.
authors.affiliation: Center for Molecular Medicine, Karolinska Institute, 17176, Stockholm, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Egeland
authors.forename: Jennifer
authors.initials: J
authors.lastname: Warner-Schmidt
authors.forename: Paul
authors.initials: P
authors.lastname: Greengard
authors.forename: Per
authors.initials: P
authors.lastname: Svenningsson
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D017072:Neostriatum / Q000378:metabolism* / Q000648:ultrastructure; D009474:Neurons / Q000000981:diagnostic imaging / Q000378:metabolism*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D044302:Receptor, Serotonin, 5-HT1B / Q000096:biosynthesis*; D044422:Receptors, Serotonin, 5-HT4 / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D009418:S100 Proteins / Q000378:metabolism*; D014463:Ultrasonography; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D049452:Green Fluorescent Proteins; D044302:Receptor, Serotonin, 5-HT1B; D044422:Receptors, Serotonin, 5-HT4; D011993:Recombinant Fusion Proteins; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C485184:enhanced green fluorescent protein