title: A high throughput amenable Arabidopsis-P. aeruginosa system reveals a rewired regulatory module and the utility to identify potent anti-infectives.
abstract: We previously demonstrated that in a metasystem consisting of Arabidopsis seedlings growing in liquid medium (in 96 well plates) even microbes considered to be innocuous such as laboratory strains of E. coli and B. subtilis can cause potent damage to the host. We further posited that such environment-induced adaptations are brought about by 'system status changes' (rewiring of pre-existing cellular signaling networks and components) of the host and the microbe, and that prolongation of such a situation could lead to the emergence of pathogenic states in real-life. Here, using this infection model, we show that the master regulator GacA of the human opportunistic pathogen P. aeruginosa (strain PA14) is dispensable for pathogenesis, as evidenced by three independent read-outs. The gene expression profile of the host after infection with wild type PA14 or the gacA mutant are also identical. GacA normally acts upstream of the quorum sensing regulatory circuit (that includes the regulator LasR) that controls a subset of virulence factors. Double mutants in gacA and lasR behave similar to the lasR mutant, as seen by abrogation of a characteristic cell type specific host cell damage caused by PA14 or the gacA mutant. This indicates that a previously unrecognized regulatory mechanism is operative under these conditions upstream of LasR. In addition, the detrimental effect of PA14 on Arabidopsis seedlings is resistant to high concentrations of the aminoglycoside antibiotic gentamicin. These data suggest that the Arabidopsis seedling infection system could be used to identify anti-infectives with potentially novel modes of action.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America. gopalans@resurfx.com
authors.forename: Suresh
authors.initials: S
authors.lastname: Gopalan
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Ausubel
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000890:Anti-Infective Agents / Q000032:analysis / Q000302:isolation & purification*; D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology* / Q000502:physiology; D055808:Drug Discovery / Q000379:methods; D020869:Gene Expression Profiling; D057166:High-Throughput Screening Assays; D054884:Host-Pathogen Interactions / Q000502:physiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D036226:Seedlings / Q000235:genetics / Q000382:microbiology / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents