title: Conventional dendritic cells mount a type I IFN response against Candida spp. requiring novel phagosomal TLR7-mediated IFN-β signaling.
abstract: Human fungal pathogens such as the dimorphic Candida albicans or the yeast-like Candida glabrata can cause systemic candidiasis of high mortality in immunocompromised individuals. Innate immune cells such as dendritic cells and macrophages establish the first line of defense against microbial pathogens and largely determine the outcome of infections. Among other cytokines, they produce type I IFNs (IFNs-I), which are important modulators of the host immune response. Whereas an IFN-I response is a hallmark immune response to bacteria and viruses, a function in fungal pathogenesis has remained unknown. In this study, we demonstrate a novel mechanism mediating a strong IFN-β response in mouse conventional dendritic cells challenged by Candida spp., subsequently orchestrating IFN-α/β receptor 1-dependent intracellular STAT1 activation and IFN regulatory factor (IRF) 7 expression. Interestingly, the initial IFN-β release bypasses the TLR 4 and TLR2, the TLR adaptor Toll/IL-1R domain-containing adapter-inducing IFN-β and the β-glucan/phagocytic receptors dectin-1 and CD11b. Notably, Candida-induced IFN-β release is strongly impaired by Src and Syk family kinase inhibitors and strictly requires completion of phagocytosis as well as phagosomal maturation. Strikingly, TLR7, MyD88, and IRF1 are essential for IFN-β signaling. Furthermore, in a mouse model of disseminated candidiasis we show that IFN-I signaling promotes persistence of C. glabrata in the host. Our data uncover for the first time a pivotal role for endosomal TLR7 signaling in fungal pathogen recognition and highlight the importance of IFNs-I in modulating the host immune response to C. glabrata.
authors.affiliation: Christian Doppler Laboratory for Infection Biology, Max F Perutz Laboratories, Medical University Vienna, A-1030 Vienna, Austria.
authors.forename: Christelle
authors.initials: C
authors.lastname: Bourgeois
authors.forename: Olivia
authors.initials: O
authors.lastname: Majer
authors.forename: Ingrid E
authors.initials: IE
authors.lastname: Frohner
authors.forename: Iwona
authors.initials: I
authors.lastname: Lesiak-Markowicz
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Hildering
authors.forename: Walter
authors.initials: W
authors.lastname: Glaser
authors.forename: Silvia
authors.initials: S
authors.lastname: Stockinger
authors.forename: Thomas
authors.initials: T
authors.lastname: Decker
authors.forename: Shizuo
authors.initials: S
authors.lastname: Akira
authors.forename: Mathias
authors.initials: M
authors.lastname: Müller
authors.forename: Karl
authors.initials: K
authors.lastname: Kuchler
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D002176:Candida albicans / Q000276:immunology*; D041221:Candida glabrata / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007370:Interferon Type I / Q000502:physiology*; D016899:Interferon-beta / Q000378:metabolism / Q000502:physiology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010588:Phagosomes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D015398:Signal Transduction / Q000276:immunology*; D051199:Toll-Like Receptor 7 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D007370:Interferon Type I; C495348:TLR7 protein, human; D051199:Toll-Like Receptor 7; D016899:Interferon-beta