title: Evaluation of tip-enhanced Raman spectroscopy for characterizing different virus strains.
abstract: Tip-enhanced Raman spectroscopy (TERS) is a highly sensitive spectroscopic technique which combines the advantages of optical spectroscopy with the requirements needed for the characterization of biological nano-structures. In this study, TERS was used to investigate the applicability of this spectroscopic technique for the detection of different virus strains like avipoxvirus and adeno-associated virus. TERS spectra obtained from different particles of the same virus strain show variations in relative peak intensities and positions of most spectral features observed. These spectral variations were higher for the larger avipoxvirus particles (∅≈350 nm) than for the smaller adeno-associated virus particles (∅≈26 nm).
authors.affiliation: P25-Biomedical Spectroscopy, Robert-Koch Institute, Nordufer 20, D-13353 Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Hermann
authors.forename: Antje
authors.initials: A
authors.lastname: Hermelink
authors.forename: Veronika
authors.initials: V
authors.lastname: Lausch
authors.forename: Gudrun
authors.initials: G
authors.lastname: Holland
authors.forename: Lars
authors.initials: L
authors.lastname: Möller
authors.forename: Norbert
authors.initials: N
authors.lastname: Bannert
authors.forename: Dieter
authors.initials: D
authors.lastname: Naumann
mesh_terms: D018150:Avipoxvirus / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D000229:Dependovirus / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014780:Viruses / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure