title: STRs, mini STRs and SNPs--a comparative study for typing degraded DNA.
abstract: Short tandem repeat (STR) systems are the most powerful and widely used genetic marker systems in forensic DNA typing. Optimized amplification conditions and PCR reagents in combination with laser fluorescence based detection methods have increased the sensitivity and decreased the detection threshold down to approximately 100 pg. The quality of human DNA from forensic samples can be influenced by environmental factors. These may cause different degrees of degradation which have a negative impact on the amplification process especially of STR systems with large amplicons. Therefore, methods which need only small amplicon sizes to detect DNA markers are a better choice for typing degraded DNA. Here we report investigations on different types of DNA markers and typing methods which should all be applicable for analysing degraded DNA. These are two commercially available mini STR kits and five SNP markers which were analysed with two self established assays, a 5' nuclease assay and a minisequencing (SNaPshot) assay. The investigations comprised sensitivity studies, different types of stain material, as well as intact and degraded DNA. Results indicate that mini STRs are superior to standard STR typing methods, especially for typing old stain material with small amounts of degraded DNA. SNP typing based on the minisequencing (SNaPshot) assay achieved a better success rate in typing aged blood and saliva stains compared to standard STRs and SNP typing using the 5' nuclease assay.
authors.affiliation: Institute of Legal Medicine, University of Bonn, Bonn, Germany. timsenge@googlemail.com
authors.forename: Tim
authors.initials: T
authors.lastname: Senge
authors.forename: Burkhard
authors.initials: B
authors.lastname: Madea
authors.forename: Anke
authors.initials: A
authors.lastname: Junge
authors.forename: Markus A
authors.initials: MA
authors.lastname: Rothschild
authors.forename: Peter M
authors.initials: PM
authors.lastname: Schneider
mesh_terms: D001774:Blood Chemical Analysis; D004247:DNA / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004586:Electrophoresis / Q000379:methods; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D012463:Saliva / Q000737:chemistry
chemical_list: D004247:DNA