title: Minocycline-released hydroxyapatite-gelatin nanocomposite and its cytocompatibility in vitro.
abstract: The incorporation of antibacterial agents into biomaterials is extremely desirable for repairing bone defects. Minocycline, a semi-synthetic tetracycline antibiotic, is active against aerobic, anaerobic, Gram-positive and Gram-negative bacteria, and can enhance bone formation, decrease connective tissue breakdown and diminish bone resorption. In this study, a novel minocycline-releasing biomaterial was synthesized using a biomimetic method. A measured amount of an acidic hydroxyapatite and minocycline solution was respectively added to a gelatin solution and kept at 40 °C and pH 7-8 for 2 h. The mixture was aged overnight, lyophilized and a hydroxyapatite-gelatin-minocycline composite was obtained. The composite was co-cultured with rat bone marrow stromal cells (BMSCs) in vitro. Our results show that nanohydroxyapatite was distributed evenly in the fibrils of the gelatin. Minocycline was incorporated into the composite and could be released from the composite particles slowly over 2 weeks in vitro. The composite promoted BMSC adhesion, proliferation and differentiation in vitro. The approach described here may provide a basis for the preparation of an antibacterial biomaterial for bone regeneration.
authors.affiliation: Key Laboratory of Oral Disease Research of Anhui Province, Stomatologic Hospital & Collage, Anhui Medical University, Hefei, People's Republic of China.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Dou
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Hua-Qiong
authors.initials: HQ
authors.lastname: Cai
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.forename: Quan-Li
authors.initials: QL
authors.lastname: Li
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry*; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D008911:Minocycline / Q000494:pharmacology*; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D005780:Gelatin; D017886:Durapatite; D008911:Minocycline