title: Assessment of impact of peptide nucleic acid fluorescence in situ hybridization for rapid identification of coagulase-negative staphylococci in the absence of antimicrobial stewardship intervention.
abstract: Peptide nucleic acid fluorescence in situ hybridization (PNA FISH) was instituted at Boston Medical Center for the rapid identification of coagulase-negative staphylococci (CoNS). Without active notification or antimicrobial stewardship intervention, a pre- and postimpact analysis showed no benefit of this assay with respect to the length of hospital stay or vancomycin use.
authors.affiliation: Department of Pharmacy, Boston Medical Center, Boston, Massachusetts, USA. carol.holtzman@temple.edu
authors.forename: Carol
authors.initials: C
authors.lastname: Holtzman
authors.forename: Dana
authors.initials: D
authors.lastname: Whitney
authors.forename: Tamar
authors.initials: T
authors.lastname: Barlam
authors.forename: Nancy S
authors.initials: NS
authors.lastname: Miller
mesh_terms: D000046:Academic Medical Centers; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001431:Bacteriological Techniques / Q000379:methods*; D001900:Boston; D003030:Coagulase / Q000378:metabolism; D004363:Drug Utilization / Q000706:statistics & numerical data; D005260:Female; D005453:Fluorescence; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007902:Length of Stay; D008297:Male; D020135:Peptide Nucleic Acids; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D016896:Treatment Outcome; D014640:Vancomycin / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D003030:Coagulase; D020135:Peptide Nucleic Acids; D014640:Vancomycin