title: The regenerative medicine laboratory: facilitating stem cell therapy for equine disease.
abstract: This article focuses on the emerging field of equine regenerative medicine with an emphasis on the use of mesenchymal stem cells (MSCs) for orthopedic diseases. We detail laboratory procedures and protocols for tissue handling and MSC isolation, characterization, expansion, and cryopreservation from bone marrow, fat, and placental tissues. We provide an overview of current clinical uses for equine MSCs and how MSCs function to heal tissues. Current laboratory practices in equine regenerative medicine mirror those in the human field. However, the translational use of autologous and allogeneic MSCs for patient therapy far exceeds what is currently permitted in human medicine.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, 95616, USA. dlborjesson@ucdavis.edu
authors.forename: Dori L
authors.initials: DL
authors.lastname: Borjesson
authors.forename: John F
authors.initials: JF
authors.lastname: Peroni
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000637:transplantation; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006734:Horse Diseases / Q000628:therapy*; D006736:Horses; D007886:Legislation, Veterinary; D045164:Mesenchymal Stem Cell Transplantation / Q000941:ethics / Q000331:legislation & jurisprudence / Q000662:veterinary*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology; D044968:Regenerative Medicine / Q000379:methods / Q000639:trends; D016378:Tissue Transplantation / Q000941:ethics / Q000331:legislation & jurisprudence / Q000662:veterinary; D020858:Tissue and Organ Harvesting / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods / Q000639:trends