title: Modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251.
abstract: In a recent directed-evolution study, Escherichia coli D-sialic acid aldolase was converted by introducing eight point mutations into a new enzyme with relaxed specificity, denoted RS-aldolase (also known formerly as L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase), which showed a preferred selectivity toward L-KDO. To investigate the underlying molecular basis, we determined the crystal structures of D-sialic acid aldolase and RS-aldolase. All mutations are away from the catalytic center, except for V251I, which is near the opening of the (α/β)(8)-barrel and proximal to the Schiff base-forming Lys-165. The change of specificity from D-sialic acid to RS-aldolase can be attributed mainly to the V251I substitution, which creates a narrower sugar-binding pocket, but without altering the chirality in the reaction center. The crystal structures of D-sialic acid aldolase·l-arabinose and RS-aldolase·hydroxypyruvate complexes and five mutants (V251I, V251L, V251R, V251W, and V251I/V265I) of the D-sialic acid aldolase were also determined, revealing the location of substrate molecules and how the contour of the active site pocket was shaped. Interestingly, by mutating Val251 alone, the enzyme can accept substrates of varying size in the aldolase reactions and still retain stereoselectivity. The engineered D-sialic acid aldolase may find applications in synthesizing unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan.
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Chou
authors.forename: Tzu-Ping
authors.initials: TP
authors.lastname: Ko
authors.forename: Kuan-Jung
authors.initials: KJ
authors.lastname: Wu
authors.forename: Kai-Fa
authors.initials: KF
authors.lastname: Huang
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Lin
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Andrew H-J
authors.initials: AH
authors.lastname: Wang
mesh_terms: D020134:Catalytic Domain; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry; D007700:Kinetics; D008239:Lysine / Q000737:chemistry; D008956:Models, Chemical; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013237:Stereoisomerism; D013379:Substrate Specificity; D014633:Valine / Q000737:chemistry*
chemical_list: D005634:Fructose-Bisphosphate Aldolase; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase; D014633:Valine; D008239:Lysine