title: Isolation and characterization of new facultative alkaliphilic Bacillus flexus strains from maize processing waste water (nejayote).
abstract: OBJECTIVE
  This work describes the isolation and characterization of two new alkaliphilic micro-organisms present in nejayote.
  
  
  RESULTS
  Samples of fresh industrial nejayote were plated on nejayote medium and incubated for 4 days at 37 °C. Isolates were identified based on morphological and physiological characteristics, as well as 16S rDNA sequence analysis. Two gram-positive strains, NJY2 and NJY4, able to hydrolyse starch, xylan, and gelatin were isolated from nejayote. Comparative sequence analysis of 16S rDNA and phylogenetic studies indicate that the micro-organisms studied were closely related to members of the Bacillus flexus species. The strains were identified as facultative alkaliphilic salt tolerant bacteria. Isolate NJY2 produced cell associated phenolic acid esterases, able to release ferulic acid from nixtamalised corn bran and ethyl and methyl esters.
  
  
  CONCLUSIONS
  The isolated strains of B. flexus NJY2 and NJY4 showed important physiological properties to produce high-value molecules from agroindustrial by-products.
  
  
  CONCLUSIONS
  This is the first report about the isolation of alkaliphilic micro-organisms from nejayote and the first report of phenolic acid esterases synthesised by alkaliphiles. The new alkaliphilic micro-organisms have potential application in the treatment and transformation of tortilla industry residues.
authors.affiliation: Area Academica Quimico Farmaceutico Biologo, Facultad de Ciencias Quimicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico. monica.sanchezgn@uanl.edu.mx
authors.forename: M
authors.initials: M
authors.lastname: Sanchez-Gonzalez
authors.forename: A
authors.initials: A
authors.lastname: Blanco-Gamez
authors.forename: A
authors.initials: A
authors.lastname: Escalante
authors.forename: A G
authors.initials: AG
authors.lastname: Valladares
authors.forename: C
authors.initials: C
authors.lastname: Olvera
authors.forename: R
authors.initials: R
authors.lastname: Parra
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D004950:Esterases / Q000378:metabolism; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology; D014876:Water Pollution; D003313:Zea mays
chemical_list: D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D004950:Esterases