title: Inhibitory effects of Terminalia chebula extract on glycation and endothelial cell adhesion.
abstract: Terminalia chebula Retz. has been used in India for a long time to treat many diseases, and its extract was reported to have antidiabetic activity in vivo. In this study, T. chebula methanolic extract (TCE) containing 2.7 % chebulic acid was evaluated for its preventive effects against the formation of advanced glycation end products (AGEs) and endothelial cell dysfunction. When the effects of TCE on AGE formation and on protein crossing-linking by glycation with D-threose and lens crystallines were examined, TCE showed inhibitory activity in a dose-dependent manner, and the concentration of 1000 µg/mL presented an activity similar to that of 5 mM aminoguanidine as a positive control. Upon investigating the protective activity of TCE against AGE-induced vascular endothelium dysfunction, human umbilical vein endothelial cells (HUVEC) incubated with 100 µg/mL of AGEs had significantly enhanced reactive oxygen species (ROS) formation, whereas the treatment of T. chebula reduced AGE-induced ROS generation. The incubation of HUVEC with 100 µg/mL of AGEs caused a considerable increase in THP-1 monocytic cell adhesion, but this adhesion was reduced by the treatment of TCE. These results suggest that TCE is a potential agent for alleviating diabetic complications.
authors.affiliation: Department of Food and Nutrition, Institute of Life Resources, Korea University, Seoul, Republic of Korea.
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Hong Yon
authors.initials: HY
authors.lastname: Cho
authors.forename: Kuen-Woo
authors.initials: KW
authors.lastname: Park
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Kim
authors.forename: Jong-Tak
authors.initials: JT
authors.lastname: Kim
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Nam
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D006801:Humans; D007908:Lens, Crystalline / Q000187:drug effects; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D029766:Terminalia; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced; D010936:Plant Extracts