title: Inhibition of Lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells.
abstract: Sargassum hemiphyllum , a kind of brown seaweed generally found along coastlines in East Asia, has long served as a traditional Chinese medicine. S. hemiphyllum has shown an anti-inflammatory effect; however, its mechanism has not been elucidated clearly. This study explored S. hemiphyllum for its biomedical effects. S. hemiphyllum sulfated polysaccharide extract (SHSP) was first prepared; the mouse macrophage cell line (RAW 264.7) activated by lipopolysaccharide (LPS) was used as a model system. The secretion profiles of pro-inflammatory cytokines, including IL-1β, IL-6, TNF-α, and NO, were found significantly to be reduced in 1-5 mg/mL dose ranges of SHSP treatments. RT-PCR analysis suggested SHSP inhibits the LPS-induced mRNA expressions of IL-β, iNOS, and COX-2 in a dose-dependent manner. At protein levels, Western blot analysis demonstrated a similar result for NF-κB (p65) in cytosol/nuclear. Taken together, the anti-inflammatory properties of SHSP may be attributed to the down-regulation of NF-κB in nucleus.
authors.affiliation: Seafood Technology Division, Fisheries Research Institute , Council of Agriculture, Keelung, Taiwan.
authors.forename: Pai-An
authors.initials: PA
authors.lastname: Hwang
authors.forename: Shih-Yung
authors.initials: SY
authors.lastname: Chien
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Chan
authors.forename: Mei-Kuang
authors.initials: MK
authors.lastname: Lu
authors.forename: Chwen-Herng
authors.initials: CH
authors.lastname: Wu
authors.forename: Zwe-Ling
authors.initials: ZL
authors.lastname: Kong
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000235:genetics; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D016328:NF-kappa B / Q000032:analysis; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D044647:Sargassum / Q000737:chemistry*; D013431:Sulfates / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011134:Polysaccharides; D012333:RNA, Messenger; D013431:Sulfates; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2