title: Adenosine 5'-triphosphate-sensitive potassium channel activator induces the up-regulation of caveolin-1 expression in a rat brain tumor model.
abstract: This study was performed to determine whether minoxidil sulfate (MS), a selective Adenosine 5'-triphosphate-sensitive potassium channel (K (ATP) channel) activator, has an effect on the expression of caveolin-1 in the rat's brain tumor tissue. Using a rat brain glioma (C6) model, we found that the expression of caveolin-1 protein at tumor sites was greatly increased after intracarotid infusion of MS at a dose of 30 μg/kg/min for 15, 30, and 60 min via Western blot analysis. And the peak value of the caveolin-1 expression was observed in rats with glioma after 15 min of MS perfusion, which was significantly attenuated by reactive oxygen species (ROS) scavenger (N-2-mercaptopropionyl glycine, MPG). In addition, MPG also significantly inhibited the increase of blood-brain tumor barrier (BTB) permeability which was induced by MS. This led to the conclusion that the MS-induced BTB permeability increase may be related to the accelerated formation of caveolin-1 protein, and could be mediated by ROS.
authors.affiliation: Department of Physiology, Life Science and Biology Pharmacopedia Institution, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning Province, People's Republic of China. yantinggu008@yahoo.com.cn
authors.forename: Yan-ting
authors.initials: YT
authors.lastname: Gu
authors.forename: Yi-xue
authors.initials: YX
authors.lastname: Xue
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xin-yuan
authors.initials: XY
authors.lastname: Liang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D001932:Brain Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism*; D004195:Disease Models, Animal; D005070:Evans Blue / Q000378:metabolism; D005910:Glioma / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D054086:KATP Channels / Q000378:metabolism*; D008914:Minoxidil / Q000031:analogs & derivatives* / Q000494:pharmacology; D010539:Permeability / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D008625:Tiopronin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D051242:Caveolin 1; D054086:KATP Channels; C039516:minoxidil sulfate ester; D005070:Evans Blue; D008914:Minoxidil; D008625:Tiopronin