title: FMP30 is required for the maintenance of a normal cardiolipin level and mitochondrial morphology in the absence of mitochondrial phosphatidylethanolamine synthesis.
abstract: Mitochondria of the yeast Saccharomyces cerevisiae contain enzymes Crd1p and Psd1p, which synthesize cardiolipin (CL) and phosphatidylethanolamine respectively. A previous study indicated that crd1Δ is synthetically lethal with psd1Δ. In this study, to identify novel genes involved in CL metabolism, we searched for genes that genetically interact with Psd1p, and found that deletion of FMP30 encoding a mitochondrial inner membrane protein results in a synthetic growth defect with psd1Δ. Although fmp30Δ cells grew normally and exhibited a slightly decreased CL level, fmp30Δpsd1Δ cells exhibited a severe growth defect and an about 20-fold reduction in the CL level, as compared with the wild-type control. We found also that deletion of FMP30 caused a defect in mitochondrial morphology. Furthermore, FMP30 genetically interacted with seven mitochondrial morphology genes. These results indicated that Fmp30p is involved in the maintenance of mitochondrial morphology and required for the accumulation of a normal level of CL in the absence of mitochondrial phosphatidylethanolamine synthesis.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Kuroda
authors.forename: Motohiro
authors.initials: M
authors.lastname: Tani
authors.forename: Akira
authors.initials: A
authors.lastname: Moriguchi
authors.forename: Sho
authors.initials: S
authors.lastname: Tokunaga
authors.forename: Takahito
authors.initials: T
authors.lastname: Higuchi
authors.forename: Sakae
authors.initials: S
authors.lastname: Kitada
authors.forename: Osamu
authors.initials: O
authors.lastname: Kuge
mesh_terms: D015153:Blotting, Western; D002308:Cardiolipins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D053078:Membrane Potential, Mitochondrial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002308:Cardiolipins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D010714:Phosphatidylethanolamines; D029701:Saccharomyces cerevisiae Proteins; C483858:phosphatidylethanolamine