title: First multicenter study for risk factors for hepatocellular carcinoma development in North Africa.
abstract: OBJECTIVE
  To assess the role of the major risk factors for hepatocellular carcinoma (HCC) development in the western part of North Africa.
  
  
  METHODS
  A multicenter case control study was conducted in Tunisia, Morocco and Algeria in collaboration with Pasteur Institutes in these countries. A total of 164 patients with HCC and 250 control subjects without hepatic diseases were included. Prevalences of HBsAg, anti-hepatitis C virus (HCV) and diabetes were assessed. HCV and HBV genotyping were performed for anti-HCV and HBsAg positive patients.
  
  
  RESULTS
  The mean age of patients was 62 ± 10 years old for a 1.5 M:F sex ratio. Sixty percent of HCC patients were positive for anti-HCV and 17.9% for HBsAg. Diabetes was detected in 18% of cases. Odd ratio (OR) and 95% confidence intervals (CI) were 32.0 (15.8 - 65.0), 7.2 (3.2 - 16.1) and 8.0 (3.1 - 20.0) for anti-HCV, HBsAg and diabetes respectively. Multivariate analysis indicated that the three studied factors were independent. 1b HCV genotype and D HBV genotype were predominant in HCC patients. HCV was the only risk factor significantly associated with an excess of cirrhosis (90% vs 68% for all other risk factors collectively, P = 0.00168). Excessive alcohol consumption was reliably established for 19 (17.6%) cases among the 108 HCC patients for whom data is available.
  
  
  CONCLUSIONS
  HCV and HBV infections and diabetes are the main determinants of HCC development in North Africa. An active surveillance and secondary prevention programs for patients with chronic hepatitis and nutrition-associated metabolic liver diseases are the most important steps to reduce the risk of HCC in the region.
authors.affiliation: Olfa Bahri, Hinda Triki, Laboratory of Clinical Virology, Institut Pasteur of Tunis, Tunis, BP 1002, Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: Bahri
authors.forename: Sayeh
authors.initials: S
authors.lastname: Ezzikouri
authors.forename: Nissaf Ben
authors.initials: NB
authors.lastname: Alaya-Bouafif
authors.forename: Fella
authors.initials: F
authors.lastname: Iguer
authors.forename: Abdallah Essaid El
authors.initials: AE
authors.lastname: Feydi
authors.forename: Hafedh
authors.initials: H
authors.lastname: Mestiri
authors.forename: Moustapha
authors.initials: M
authors.lastname: Benazzouz
authors.forename: Tahar
authors.initials: T
authors.lastname: Khalfallah
authors.forename: Rajaa
authors.initials: R
authors.lastname: Afifi
authors.forename: Latifa
authors.initials: L
authors.lastname: Elkihal
authors.forename: Salah
authors.initials: S
authors.lastname: Berkane
authors.forename: Agnes
authors.initials: A
authors.lastname: Marchio
authors.forename: Nabil
authors.initials: N
authors.lastname: Debzi
authors.forename: Anne
authors.initials: A
authors.lastname: Dejean
authors.forename: Pascal
authors.initials: P
authors.lastname: Pineau
authors.forename: Hinda
authors.initials: H
authors.lastname: Triki
authors.forename: Soumaya
authors.initials: S
authors.lastname: Benjelloun