title: Studies on Escherichia coli HflKC suggest the presence of an unidentified λ factor that influences the lysis-lysogeny switch.
abstract: BACKGROUND
  The lysis-lysogeny decision in the temperate coliphage λ is influenced by a number of phage proteins (CII and CIII) as well as host factors, viz. Escherichia coli HflB, HflKC and HflD. Prominent among these are the transcription factor CII and HflB, an ATP-dependent protease that degrades CII. Stabilization of CII promotes lysogeny, while its destabilization induces the lytic mode of development. All other factors that influence the lytic/lysogenic decision are known to act by their effects on the stability of CII. Deletion of hflKC has no effect on the stability of CII. However, when λ infects ΔhflKC cells, turbid plaques are produced, indicating stabilization of CII under these conditions.
  
  
  RESULTS
  We find that CII is stabilized in ΔhflKC cells even without infection by λ, if CIII is present. Nevertheless, we also obtained turbid plaques when a ΔhflKC host was infected by a cIII-defective phage (λcIII67). This observation raises a fundamental question: does lysogeny necessarily correlate with the stabilization of CII? Our experiments indicate that CII is indeed stabilized under these conditions, implying that stabilization of CII is possible in ΔhflKC cells even in the absence of CIII, leading to lysogeny.
  
  
  CONCLUSIONS
  We propose that a yet unidentified CII-stabilizing factor in λ may influence the lysis-lysogeny decision in ΔhflKC cells.
authors.affiliation: Department of Biochemistry, Bose Institute, P 1/12, C,I,T, Scheme VIIM, Kolkata 700 054, India.
authors.forename: Kaustav
authors.initials: K
authors.lastname: Bandyopadhyay
authors.forename: Pabitra K
authors.initials: PK
authors.lastname: Parua
authors.forename: Ajit B
authors.initials: AB
authors.lastname: Datta
authors.forename: Pradeep
authors.initials: P
authors.lastname: Parrack
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics / Q000821:virology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D017353:Gene Deletion; D008242:Lysogeny; D010455:Peptides / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; C512893:HflC protein, E coli; C512891:HflK protein, E coli; D010455:Peptides; D014157:Transcription Factors; D014764:Viral Proteins; C039000:cII protein, bacteriophage lambda