title: Serological response of guinea pigs to oily and aqueous inactivated vaccines containing a Brazilian isolate of the Bovine Viral Diarrhea Virus (BVDV).
abstract: Bovine Viral Diarrhea Virus (BVDV) is widespread in cattle in Brazil and research shows its large antigenic variability. Available vaccines are produced with virus strains isolated in other countries and may not be effective. In this study, inactivated vaccines containing the Brazilian BVDV-Ib IBSP11 isolate were developed and tested on 6 groups of 10 guinea pigs (Cavia porcellus). Animals in groups A and C received an aqueous vaccine (aluminum hydroxide); B and D groups received an oily vaccine (Montanide ISA50); Group E positive-control animals were given an imported commercial vaccine with BVDV-Ia Singer; Group F animals were sham vaccinated (negative control). Groups A, B and E received two doses, and Groups C and D, three, every 21 days. Twelve blood samples were taken, at 21-day intervals over 231 days, and evaluated for antibody titer through virus-neutralization (VN), using a homologous strain (IBSP11), and a heterologous strain (BVDV-Ia NADL). Most animals, 42 days following the first dose, seroconverted to both strains and, after the second dose, there was a significant increase of titers in all groups. The oily formulation induced greater response after the third administration. This increase was not observed with the aqueous vaccines, regardless of the virus used in the VN. Antibody decline was more rapid in animals that received aqueous vaccines. The results showed the importance of studying the influence of endemic strains of commercial vaccines, to improve the efficacy of BVD vaccination. Use of the endemic strain in vaccine formulation presented promising results, as well as the use of guinea pigs as a laboratory model.
authors.affiliation: Laboratório de Produção de Imunobiológicos, Instituto Biológico, Av Cons Rodrigues Alves 1252, 04014-002 São Paulo, Brazil. jordao@biologico.sp.gov.br
authors.forename: Ricardo Spacagna
authors.initials: RS
authors.lastname: Jordão
authors.forename: Cláudia Pestana
authors.initials: CP
authors.lastname: Ribeiro
authors.forename: Edviges Maristela
authors.initials: EM
authors.lastname: Pituco
authors.forename: Líria Hiromi
authors.initials: LH
authors.lastname: Okuda
authors.forename: Cláudia
authors.initials: C
authors.lastname: Del Fava
authors.forename: Eliana de
authors.initials: Ed
authors.lastname: Stefano
authors.forename: Moacir Marchiori
authors.initials: MM
authors.lastname: Filho
authors.forename: Dolores Ursula
authors.initials: DU
authors.lastname: Mehnert
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology* / Q000821:virology; D001938:Brazil; D002417:Cattle; D002626:Chemistry, Pharmaceutical; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005838:Genotype; D006168:Guinea Pigs; D056724:Immunity, Humoral; D007279:Injections, Subcutaneous / Q000662:veterinary; D023421:Models, Animal; D009500:Neutralization Tests / Q000662:veterinary; D011897:Random Allocation; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines