title: [Dynamic observation on splenocyte subsets in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus].
abstract: OBJECTIVE
  To dynamically observe changes of subsets of splenocytes in mice immunized with recombinant Bifidobacteria bifidum (Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus (Eg).
  
  
  METHODS
  BALB/c mice were vaccinated by 5 x 10(8) colony forming unit (CFU) orally and 5 x 10(5) CFU intranasally respectively. Mice were killed on week 0, 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 after immunization respectively, and spleens were separated for cell preparation. CD4+ and CD8+ T cells were determined by flow cytometry (FCM), with MRS as control.
  
  
  RESULTS
  In the oral immunization group, CD4+ cells showed a significant increase during the 4th-10th week after vaccination, and reached the highest level on the 6th week, whereas no obvious changes in CD8+ cells numbers were observed. In the intranasal immunization group, CD4+ cells showed an obvious increase during the 4th-8th week after vaccination, and reached the highest level on the 6th week, CD8+ subsets had no obvious changes.
  
  
  CONCLUSIONS
  CD4+ T cell cells may play a key role in immune response in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.
authors.affiliation: Institute of Infectious and Parasitic Diseases, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Bi-ying
authors.initials: BY
authors.lastname: Zhou
authors.forename: Ya-tang
authors.initials: YT
authors.lastname: Chen
authors.forename: Wen-gui
authors.initials: WG
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D001644:Bifidobacterium / Q000235:genetics / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004443:Echinococcosis / Q000276:immunology* / Q000517:prevention & control; D048209:Echinococcus granulosus / Q000276:immunology*; D005260:Female; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; C446034:EG95 protein, Echinococcus granulosus; C401864:EgA31 protein, Echinococcus granulosus; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic