title: Role of the acid-sensing ion channel 3 in blood volume control.
abstract: BACKGROUND
  The mechanically sensitive volume receptors, primarily located in the venoatrial junction area, are essential for blood volume homeostasis. However, the molecular basis of the volume receptors is still unknown.
  
  
  RESULTS
  We hypothesized that the acid-sensing ion channel 3 (ASIC3) might be a candidate for the mechanically sensitive molecules expressed in the volume receptors. We examined the effect of Asic3 null mutation (Asic3(-/-)) on blood volume expansion (BVE)-induced urine flow, neural activation, and atrial natriuretic peptide (ANP) release in mice. BVE-induced urine flow was lower in Asic3(-/-) mice than in wild-type littermates. In addition, the stretch-activated channel blocker GdCl(3) further reduced the BVE-induced urine flow in Asic3(-/-) mice. BVE increased phosphorylated extracellular signal-related kinase (pERK) immunoreactivity in nodose ganglia and many segments of dorsal root ganglia (DRG) in all mice, but pERK-positive neurons were fewer in Asic3(-/-) mice or mice pretreated with GdCl(3) than in wild-type mice. Asic3 knockout selectively decreased BVE-induced pERK-immunoreactive neurons in nodose ganglia, and in C8 and T2 DRG. Moreover, BVE increased the circulating ANP level, which was abolished in Asic3(-/-) mice and wild-type mice treated with GdCl(3). Asic3 knockout reduced the BVE-induced plasma ANP elevation in a GdCl(3)-independent manner.
  
  
  CONCLUSIONS
  ASIC3 is a molecular substrate involved in detecting the vessel stretch caused by BVE.
authors.affiliation: Institute of Neuroscience, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Lee
authors.forename: Synthia H
authors.initials: SH
authors.lastname: Sun
authors.forename: Shing-Hong
authors.initials: SH
authors.lastname: Lin
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chen
mesh_terms: D062565:Acid Sensing Ion Channels; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001810:Blood Volume / Q000187:drug effects / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D005682:Gadolinium / Q000494:pharmacology; D051379:Mice; D008817:Mice, Mutant Strains; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009620:Nodose Ganglion / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects / Q000502:physiology; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: C508378:ASIC3 protein, mouse; D062565:Acid Sensing Ion Channels; D000893:Anti-Inflammatory Agents; D009419:Nerve Tissue Proteins; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D005682:Gadolinium; D048049:Extracellular Signal-Regulated MAP Kinases; C038958:gadolinium chloride