title: Effect of carbon nanotube coating of aligned nanofibrous polymer scaffolds on the neurite outgrowth of PC-12 cells.
abstract: Neurite outgrowth from endogenous or transplanted cells is important for neural regeneration following nerve tissue injury. Modified substrates often provide better environments for cell adhesion and neurite outgrowth. This study was conducted to determine if MWCNT (multiwalled carbon nanotube)-coated electrospun PLCL [poly (l-lactic acid-co-3-caprolactone)] nanofibres improved the neurite outgrowth of PC-12 cells. To accomplish this, two groups, PC-12 cells in either uncoated PLCL scaffolds or MWCNT-coated PLCL scaffolds were cultured for 9 days. MWCNT-coated PLCL scaffolds showed improved adhesion, proliferation and neurite outgrowth of PC-12 cells. These findings suggest that MWCNT-coated nanofibrous scaffolds may be an attractive platform for cell transplantation application in neural tissue engineering.
authors.affiliation: Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, South Korea.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Jin
authors.forename: Meeju
authors.initials: M
authors.lastname: Kim
authors.forename: Ueon Sang
authors.initials: US
authors.lastname: Shin
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D002208:Caproates / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry; D007783:Lactones / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009416:Nerve Regeneration; D016501:Neurites / Q000187:drug effects*; D016716:PC12 Cells; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D002208:Caproates; D020099:Coated Materials, Biocompatible; D007783:Lactones; D037742:Nanotubes, Carbon; D011108:Polymers; D019344:Lactic Acid; C121056:caprolactone