title: Determination of organic acids in tissues and exudates of maize, lupin, and chickpea by high-performance liquid chromatography-tandem mass spectrometry.
abstract: This article describes a fast and simple methodology for the extraction and determination of organic acids in tissues and root exudates of maize, lupin, and chickpea by LC/MS/MS. Its main advantage is that it does not require sample prepurification before HPLC analysis or sample derivatization to improve sensibility. The results obtained showed good precision and accuracy, a recovery close to 100%, and no significant matrix effect. Moreover, the sensibility of the method is in general better than that of previously described methodologies, with detection limits between 15 and 900 pg injected.
authors.affiliation: Department of Chemistry and Soil Chemistry, University of Navarra, 31080 Pamplona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Erro
authors.forename: Angel M
authors.initials: AM
authors.lastname: Zamarreño
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Yvin
authors.forename: Jose M
authors.initials: JM
authors.lastname: Garcia-Mina
mesh_terms: D002264:Carboxylic Acids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029863:Cicer / Q000737:chemistry*; D031297:Lupinus / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D018520:Plant Shoots / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002264:Carboxylic Acids