title: Association of stem cell-related markers and survival in astrocytic gliomas.
abstract: To study the clinical relevance of undifferentiated tumour cells in astrocytic gliomas we employed a large tumour tissue microarray (n=283) with corresponding clinical data and analyzed the expression of Nestin and Sox-2, which mark undifferentiated stem- and progenitor cells in the normal brain. Both markers were expressed abundantly and staining of nestin significantly increased with WHO grade. Further, nestin and Sox-2 immunoreactivity was significantly associated with tumour cell proliferation and nestin expression was independently associated with poor patient survival. Our findings suggest that immature glioma cells are involved in tumour growth and tumour progression and significantly impact on patient prognosis.
authors.affiliation: Division of Neurosurgical Research, Department of Neurosurgery, University of Heidelberg, INF 400, 69120 Heidelberg, Germany.
authors.forename: Feng
authors.initials: F
authors.lastname: Wan
authors.forename: Christel
authors.initials: C
authors.lastname: Herold-Mende
authors.forename: Benito
authors.initials: B
authors.lastname: Campos
authors.forename: Franz-Simon
authors.initials: FS
authors.lastname: Centner
authors.forename: Christine
authors.initials: C
authors.lastname: Dictus
authors.forename: Natalia
authors.initials: N
authors.lastname: Becker
authors.forename: Frauke
authors.initials: F
authors.lastname: Devens
authors.forename: Carolin
authors.initials: C
authors.lastname: Mogler
authors.forename: Jörg
authors.initials: J
authors.lastname: Felsberg
authors.forename: Niels
authors.initials: N
authors.lastname: Grabe
authors.forename: Guido
authors.initials: G
authors.lastname: Reifenberger
authors.forename: Peter
authors.initials: P
authors.lastname: Lichter
authors.forename: Andreas
authors.initials: A
authors.lastname: Unterberg
authors.forename: Justo Lorenzo
authors.initials: JL
authors.lastname: Bermejo
authors.forename: Rezvan
authors.initials: R
authors.lastname: Ahmadi
mesh_terms: D000328:Adult; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D008137:Longitudinal Studies; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D064231:Nestin; D011379:Prognosis; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D055748:SOXB1 Transcription Factors