title: [Effects of cyclosporine A on pneumocyte apoptosis with lung ischemia/reperfusion injury in rats].
abstract: OBJECTIVE
  To investigate the effects of cyclosporine A (CsA), a powerful inhibitor of mitochondrial permeability transition pore (MPTP), on pneumocyte apoptosis, the release of cytochrome C and the activity of caspase-3 after lung ischemia/reperfusion, and explore the mechanisms.
  
  
  METHODS
  Single lung in situ ischemia/reperfusion animal model was used. 30 SD rats were randomly divided into three groups (n = 10): sham (S) group, ischemia/reperfusion (I/R) group and cyclosporine A (CsA) group. Apoptosis of pneumocyte was assessed by TUNEL method, cytochrome C (CytC) in cytoplasm was detected by immunohistochemistry techniques, and the activity of caspase-3 was measured with spectrophotometer.
  
  
  RESULTS
  The content of CytC in cytoplasm, the activity of caspase-3, and the value of apoptosis index (AI) in ischemia/reperfusion group were evidently higher than that in S group (P < 0.01). CsA suppressed apoptosis as well as CytC release and caspase-3 activity (P < 0.01).
  
  
  CONCLUSIONS
  CsA can prevent the release of cytochrome C, block the apoptosis of pneumocyte accordingly maybe by closing the MPTP.
authors.affiliation: Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Yong-Yue
authors.initials: YY
authors.lastname: Dai
authors.forename: Ren-Wu
authors.initials: RW
authors.lastname: Zhu
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Ni
authors.forename: Mao-Lin
authors.initials: ML
authors.lastname: Hao
mesh_terms: D056809:Alveolar Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D045304:Cytochromes c / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology*
chemical_list: D016572:Cyclosporine; D045304:Cytochromes c; C505410:Casp3 protein, rat; D053148:Caspase 3