title: [Visualization and identification of hepatitis C viral particles by atomic force microscopy combined with MS/MS analysis].
abstract: Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: (1) AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; (2) mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcore(im) complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.
authors.forename: A L
authors.initials: AL
authors.lastname: Kaĭsheva
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Ivanov
authors.forename: V G
authors.initials: VG
authors.lastname: Zgoda
authors.forename: P A
authors.initials: PA
authors.lastname: Frantsuzov
authors.forename: T O
authors.initials: TO
authors.lastname: Pleshakova
authors.forename: N V
authors.initials: NV
authors.lastname: Krokhin
authors.forename: V S
authors.initials: VS
authors.lastname: Ziborov
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D016174:Hepacivirus / Q000276:immunology; D006526:Hepatitis C / Q000097:blood; D018936:Hepatitis C Antigens / Q000032:analysis / Q000276:immunology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014771:Virion / Q000276:immunology; D014773:Virology / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D018936:Hepatitis C Antigens; D011994:Recombinant Proteins