title: Isolation of Histophilus somni from the nasal exudates of a clinically healthy adult goat.
abstract: METHODS
  The nasal exudate from 42 goats of the Mixteca Region in the state of Puebla, Mexico, was evaluated. A strain was isolated after 4 days of incubation. This strain was identified according to its phenotypic characteristics and by means of a species-specific polymerase chain reaction (PCR), as well as by sequencing of the amplified product.
  
  
  RESULTS
  The species-specific PCR amplified a 407-bp fragment of 16S RNAr subunit, and the product sequencing revealed 100% homology with Histophilus somni 129PT. The nucleotide sequence was deposited in the GenBank under accession number HM032735.
  
  
  CONCLUSIONS
  This is the first worldwide isolation of H. somni from nasal exudates of a clinically healthy goat.
authors.affiliation: CENID Microbiología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal México-Toluca Km. 15.5, Cuajimalpa, Mexico.
authors.forename: Nelson
authors.initials: N
authors.lastname: Pérez-Romero
authors.forename: Francisco
authors.initials: F
authors.lastname: Aguilar-Romero
authors.forename: Beatriz
authors.initials: B
authors.lastname: Arellano-Reynoso
authors.forename: Efrén
authors.initials: E
authors.lastname: Díaz-Aparicio
authors.forename: Rigoberto
authors.initials: R
authors.lastname: Hernández-Castro
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D006041:Goats / Q000382:microbiology*; D044138:Haemophilus somnus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008800:Mexico; D008969:Molecular Sequence Data; D009296:Nasal Cavity / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S