title: C-type lectin from red swamp crayfish Procambarus clarkii participates in cellular immune response.
abstract: Lectins are potential immune recognition proteins. In this study, a novel C-type lectin (Pc-Lec1) is reported in freshwater crayfish Procambarus clarkii. Pc-Lec1 encodes a protein of 163 amino acids with a putative signal peptide and a single carbohydrate recognition domain. It was constitutively expressed in various tissues of a normal crayfish, especially in the hepatopancreas and gills. Expressions of Pc-Lec1 were up-regulated in the hepatopancreas and gills of crayfish challenged with Vibrio anguillarum, Staphylococcus aureus, or the white spot syndrome virus. Recombinant mature Pc-Lec1 bound bacteria and polysaccharides (peptidoglycan, lipoteichoic acid, and lipopolysaccharide) but did not agglutinate bacteria. Pc-Lec1 enhanced hemocyte encapsulation of the sepharose beads in vitro, and the blocking of beads by a polyclonal antibody inhibited encapsulation. Pc-Lec1 promoted clearance of V. anguillarum in vivo. These results suggest that Pc-Lec1 is a pattern recognition receptor and participates in cellular immune response. Pc-Lec1 performs its function as an opsonin by enhancing the encapsulation or clearance of pathogenic bacteria.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhang
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Sun
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Zhao
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000371:Agglutination / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000276:immunology*; D003001:Cloning, Molecular; D005880:Gills / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D054884:Host-Pathogen Interactions; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000276:immunology; D014733:Vibrio / Q000276:immunology; D046848:White spot syndrome virus 1 / Q000276:immunology
chemical_list: D037181:Lectins, C-Type