title: Study of the interaction between N-confused porphyrin and bovine serum albumin by fluorescence spectroscopy.
abstract: The fluorescence and ultraviolet spectroscopy were explored to study the interaction between N-confused porphyrins (NCP) and bovine serum albumin (BSA) under imitated physiological condition. The experimental results indicated that the fluorescence quenching mechanism between BSA and NCP was static quenching procedure at low NCP concentration at 293 and 305 K or a combined quenching (static and dynamic) procedure at higher NCP concentration at 305 K. The binding constants, binding sites and the corresponding thermodynamic parameters ΔH, ΔS, and ΔG were calculated at different temperatures. The comparison of binding potency of the three NCP to BSA showed that the substituting groups in benzene ring could enhance the binding affinity. From the thermodynamic parameters, we concluded that the action force was mainly hydrophobic interaction. The binding distances between NCP and BSA were calculated using Förster non-radiation energy transfer theory. In addition, the effect of NCP on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy.
authors.affiliation: Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Yu
authors.forename: Ronghua
authors.initials: R
authors.lastname: Liu
authors.forename: Rongqiong
authors.initials: R
authors.lastname: Yi
authors.forename: Fengxian
authors.initials: F
authors.lastname: Yang
authors.forename: Haowen
authors.initials: H
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Danhong
authors.initials: D
authors.lastname: Ji
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Li
authors.forename: Pinggui
authors.initials: P
authors.lastname: Yi
mesh_terms: D000818:Animals; D002417:Cattle; D015394:Molecular Structure; D056951:Photoelectron Spectroscopy; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D011166:Porphyrins; D012710:Serum Albumin, Bovine