title: Determination of calcium, magnesium, sodium, and potassium in foodstuffs by using a microsampling flame atomic absorption spectrometric method after closed-vessel microwave digestion: method validation.
abstract: This paper describes a validation process in compliance with the NFIEN ISO/IEC 17025 standard for the determination of the macrominerals calcium, magnesium, sodium, and potassium in foodstuffs by microsampling with flame atomic absorption spectrometry after closed-vessel microwave digestion. The French Standards Commission (Agence Francaise de Normalisation) standards NF V03-110, NF EN V03-115, and XP T-90-210 were used to evaluate this method. The method was validated in the context of an analysis of the 1322 food samples of the second French Total Diet Study (TDS). Several performance criteria (linearity, LOQ, specificity, trueness, precision under repeatability conditions, and intermediate precision reproducibility) were evaluated. Furthermore, the method was monitored by several internal quality controls. The LOQ values obtained (25, 5, 8.3, and 8.3 mg/kg for Ca, Mg, Na, and K, respectively) were in compliance with the needs of the TDS. The method provided accurate results as demonstrated by a repeatability CV (CVr) of < 7% and a reproducibility CV (CVR) of < 12% for all the elements. Therefore, the results indicated that this method could be used in the laboratory for the routine determination of these four elements in foodstuffs with acceptable analytical performance.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments, Unité Contaminants Inorganiques et Minéraux de l'Environnement, 23, Avenue du Général de Gaulle, F-94706 Maisons-Alfort Cedex, France.
authors.forename: Rachida
authors.initials: R
authors.lastname: Chekri
authors.forename: Laurent
authors.initials: L
authors.lastname: Noël
authors.forename: Christelle
authors.initials: C
authors.lastname: Vastel
authors.forename: Sandrine
authors.initials: S
authors.lastname: Millour
authors.forename: Ali
authors.initials: A
authors.lastname: Kadar
authors.forename: Thierry
authors.initials: T
authors.lastname: Guérin
mesh_terms: D002118:Calcium / Q000032:analysis; D002138:Calibration; D005504:Food Analysis / Q000379:methods*; D005602:France; D007202:Indicators and Reagents; D008274:Magnesium / Q000032:analysis; D008670:Metals / Q000032:analysis*; D008872:Microwaves; D011188:Potassium / Q000032:analysis; D017419:Potassium, Dietary / Q000032:analysis; D015203:Reproducibility of Results; D012964:Sodium / Q000032:analysis; D013054:Spectrophotometry, Atomic
chemical_list: D007202:Indicators and Reagents; D008670:Metals; D017419:Potassium, Dietary; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium