title: Protein adsorption at charged surfaces: the role of electrostatic interactions and interfacial charge regulation.
abstract: The understanding of protein adsorption at charged surfaces is important for a wide range of scientific disciplines including surface engineering, separation sciences and pharmaceutical sciences. Compared to chemical entities having a permanent charge, the adsorption of small ampholytes and proteins is more complicated as the pH near a charged surface can be significantly different from the value in bulk solution. In this work, we have developed a phenomenological adsorption model which takes into account the combined role of interfacial ion distribution, interfacial charge regulation of amino acids in the proximity of the surface, electroneutrality, and mass balance. The model is straightforward to apply to a given set of experimental conditions as most model parameters are obtained from bulk properties and therefore easy to estimate or are directly measurable. The model provides a detailed understanding of the importance of surface charge on adsorption and in particular of how changes in surface charge, concentration, and surface area may affect adsorption behavior. The model is successfully used to explain the experimental adsorption behavior of the two model proteins lysozyme and α-lactalbumin. It is demonstrated that it is possible to predict the pH and surface charge dependent adsorption behavior from experimental or theoretical estimates of a preferred orientation of a protein at a solid charged interface.
authors.affiliation: Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen , Universitetsparken 2, DK-2100 Copenhagen, Denmark.
authors.forename: Rune A
authors.initials: RA
authors.lastname: Hartvig
authors.forename: Marco
authors.initials: M
authors.lastname: van de Weert
authors.forename: Jesper
authors.initials: J
authors.lastname: Østergaard
authors.forename: Lene
authors.initials: L
authors.lastname: Jorgensen
authors.forename: Henrik
authors.initials: H
authors.lastname: Jensen
mesh_terms: D000327:Adsorption; D006863:Hydrogen-Ion Concentration; D007768:Lactalbumin / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D007768:Lactalbumin; D009113:Muramidase