title: An in vitro effect of coffee on the antigen-specific immune responses of naïve splenocytes.
abstract: Coffee is a globally consumed beverage with potential health benefits. However, there are few reports about the effects of coffee on immunological functions. We previously reported that in an allergic mouse model, coffee intake prevented allergy development through augmentation of interleukin (IL)-12p40. In order to investigate the anti-allergic activity of coffee, we examined the effect of coffee on antigen (Ag)-specific responses of immune cells in vitro. Coffee treatment suppressed proliferation and IL-2 secretion of mouse splenocytes in the same way as splenocytes from mice administered coffee orally. However, IL-12p40 secretion decreased significantly as a result of in vitro coffee treatment, which was contrary to the results obtained from experiments of mice administered coffee orally. Therefore, modification associated with oral administration might influence the anti-allergic activity of coffee.
authors.affiliation: National Agriculture and Food Research Organization National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Goto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takano-Ishikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shinmoto
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000276:immunology*; D049109:Cell Proliferation; D003069:Coffee / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D007155:Immunologic Factors / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D003069:Coffee; D016207:Cytokines; D007155:Immunologic Factors; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin