title: Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation.
abstract: Tuberization in cassava (Manihot esculenta Crantz) occurs simultaneously with plant development, suggesting competition of photoassimilate partitioning between the shoot and the root organs. In potato, which is the most widely studied tuber crop, there is ample evidence suggesting that metabolism and regulatory processes in leaf may have an impact on tuber formation. To search for leaf proteins putatively involved in regulating tuber generation and/or development in cassava, comparative proteomic approaches have been applied to monitor differentially expressed leaf proteins during root transition from fibrous to tuberous. Stringent cross comparison and statistical analysis between two groups with different plant ages using Student's t test with 95% significance level revealed a number of protein spots whose abundance were significantly altered (P < 0.05) during week 4 to week 8 of growth. Of these, 39 spots were successfully identified by ion trap LC-MS/MS. The proteins span various functional categories from antioxidant and defense, carbohydrate metabolism, cyanogenesis, energy metabolism, miscellaneous and unknown proteins. Results suggested possible metabolic switches in the leaf that may trigger/regulate storage root initiation and growth. This study provides a basis for further functional characterization of differentially expressed leaf proteins, which can help understand how biochemical processes in cassava leaves may be involved in storage root development.
authors.affiliation: Department of Biochemistry, Faculty of Science, Center for Cassava Molecular Biotechnology, Center for Excellence in Protein Structure and Function, Mahidol University, 272 Rama 6 Rd., Bangkok 10400, Thailand.
authors.forename: Mashamon
authors.initials: M
authors.lastname: Mitprasat
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Surasak
authors.initials: S
authors.lastname: Jiemsup
authors.forename: Opas
authors.initials: O
authors.lastname: Boonseng
authors.forename: Kittisak
authors.initials: K
authors.lastname: Yokthongwattana
mesh_terms: D002365:Manihot / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis* / Q000096:biosynthesis; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D035281:Plant Tubers / Q000502:physiology; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D013785:Thailand
chemical_list: D010940:Plant Proteins; D020543:Proteome