title: A Phellinus baumii extract reduces obesity in high-fat diet-fed mice and absorption of triglyceride in lipid-loaded mice.
abstract: This study evaluated the anti-obesity effects of Phellinus baumii extract (PBE) in high-fat diet (HFD)-fed mice. Male 8-week-old C57BL/6 mice were randomly divided into four groups: control, normal chow diet plus vehicle; HFD-control, high-fat plus vehicle; HFD plus orlistat (Xenical(®), Roche, Basel, Switzerland) (50 mg/kg); and HFD plus PBE (500 mg/kg). PBE was administered daily by oral gavage for 12 weeks. Oral administration of PBE (500 mg/kg) significantly reduced body weight gain, hepatic lipid concentrations, and fat accumulation in epididymal adipocytes compared with mice fed HFD alone (P < .05). mRNA expression of genes related to triglyceride (TG) synthesis was suppressed in the PBE groups, and fatty acid synthase activity was also significantly inhibited (P < .05). Furthermore, we evaluated the effect of PBE on TG absorption and detected marked reduction in TG absorption in Xenical- and PBE-treated mice compared with the control group (P < .05). To determine the active compound of PBE, fractionation was conducted, and interfungin A, davallialactone, and hypholomine B were identified as the main compounds. Among the three identified compounds, as a representative compound, davallialactone was also shown to suppress fat accumulation in an in vitro model system. These anti-obesity and hypolipidemic effects appear to be partly mediated by suppressing plasma and hepatic fat accumulation through the inhibition of enzymes associated with hepatic and intestinal lipid absorption and synthesis.
authors.affiliation: Animal Model Center, Korea Research Institute of Bioscience and Biotechnology, Iksan, Jeonbuk, Republic of Korea.
authors.forename: Jung-Ran
authors.initials: JR
authors.lastname: Noh
authors.forename: In-Kyoung
authors.initials: IK
authors.lastname: Lee
authors.forename: Sun-Yung
authors.initials: SY
authors.lastname: Ly
authors.forename: Keum-Jin
authors.initials: KJ
authors.lastname: Yang
authors.forename: Gil-Tae
authors.initials: GT
authors.lastname: Gang
authors.forename: Yong-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Hwang
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D004041:Dietary Fats / Q000009:adverse effects; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007783:Lactones / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000077403:Orlistat; D008517:Phytotherapy; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D014280:Triglycerides / Q000235:genetics / Q000378:metabolism*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D019440:Anti-Obesity Agents; D001688:Biological Products; D004041:Dietary Fats; D007783:Lactones; D012333:RNA, Messenger; D014280:Triglycerides; D000077403:Orlistat; D064429:Fatty Acid Synthases