title: CD154-CD40 interactions in the control of murine B cell hematopoiesis.
abstract: Interactions between CD40 and CD154 play a very important role in control of immune responses, including the delivery of T cell help to B cells and other APCs. Thus far, there has been no role postulated for CD40-CD154 interactions in hematopoiesis. We show here that CD40 is expressed on murine pro-B cells and that its ligation enhances pro-B cell proliferation in vitro and in vivo. In addition, CD154 mRNA is present in the BM. Moreover, we show that a deficiency in CD154 expression has effects on B cell hematopoiesis. Aged, CD154-deficient mice have significantly lower levels of B hematopoietic subsets downstream of pro-B cells in the BM. In addition, B lineage cells reconstitute more slowly following BMT into CD154-deficient recipients. We hypothesize that CD154 is expressed by radio-resistant cells in the BM and plays a role in fine-tuning B cell hematopoiesis.
authors.affiliation: Department of Infection and Immunity, University of Sheffield Medical School, Sheffield, United Kingdom.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Carlring
authors.forename: Hala M
authors.initials: HM
authors.lastname: Altaher
authors.forename: Susan
authors.initials: S
authors.lastname: Clark
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Latimer
authors.forename: Tracey
authors.initials: T
authors.lastname: Jenner
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Buckle
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Heath
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D019013:CD40 Antigens / Q000378:metabolism*; D023201:CD40 Ligand / Q000378:metabolism*; D049109:Cell Proliferation; D018920:Coculture Techniques; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006410:Hematopoiesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D054448:Precursor Cells, B-Lymphoid / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019013:CD40 Antigens; D012333:RNA, Messenger; D023201:CD40 Ligand