title: Ordered nano-structure of a stamped self-organized protein layer on a HOPG surface using a HFB carrier.
abstract: A groundbreaking method for ordered molecular layer preparation on a solid surface employing the drop-stamp method has been developed by us taking advantage of the characteristics of the HFB molecule as a self-organizer/adsorption carrier. It is a smart method which can be used to prepare a self-organized protein layer on a solid surface without unspecific adsorption or defects. In our previous report, we clarified the self-organizing nature of HFB-tagged protein molecules on a surface of a solution droplet. In this report, a protein layer was prepared on a HOPG surface by using the drop-stamp method with a maltose binding protein (MBP)-tagged HFBII molecule. The structure of the stamped protein layer was investigated using frequency modulation atomic force microscopy (FM-AFM) in a liquid condition. The FM-AFM images show that the drop-stamp method can prepare an ordered protein layer on a solid surface smartly. The drop-stamp method using a HFB carrier is a practical method which can be used to prepare an ordered protein layer on a solid substrate surface without unspecific adsorption defects.
authors.affiliation: Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka 808-0196, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Iwanaga
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Asakawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukuma
authors.forename: Momoka
authors.initials: M
authors.lastname: Nakamichi
authors.forename: Sakurako
authors.initials: S
authors.lastname: Shigematsu
authors.forename: Markus B
authors.initials: MB
authors.lastname: Linder
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Haruyama
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D058619:Maltose-Binding Proteins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011506:Proteins