title: AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree.
abstract: Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760 mg/kg), alternariol monomethyl ether (AME, up to 1860 mg/kg), and alternariol (up to 70 mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease.
authors.affiliation: Institute of Sciences of Food Production, Research National Council (ISPA-CNR), Via G. Amendola 122/O, 70126 Bari, Italy. stefania.somma@ispa.cnr.it
authors.forename: Stefania
authors.initials: S
authors.lastname: Somma
authors.forename: Graciela
authors.initials: G
authors.lastname: Pose
authors.forename: Alejandro
authors.initials: A
authors.lastname: Pardo
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Mulè
authors.forename: Virginia Fernandez
authors.initials: VF
authors.lastname: Pinto
authors.forename: Antonio
authors.initials: A
authors.lastname: Moretti
authors.forename: Antonio Francesco
authors.initials: AF
authors.lastname: Logrieco
mesh_terms: D000528:Alternaria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity; D054458:Amplified Fragment Length Polymorphism Analysis; D001118:Argentina; D004271:DNA, Fungal / Q000235:genetics; D005638:Fruit / Q000382:microbiology; D014644:Genetic Variation; D007783:Lactones / Q000378:metabolism; D018551:Solanum lycopersicum / Q000382:microbiology*; D009183:Mycotoxins / Q000096:biosynthesis*; D010935:Plant Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013720:Tenuazonic Acid / Q000096:biosynthesis
chemical_list: D004271:DNA, Fungal; D007783:Lactones; D009183:Mycotoxins; D013720:Tenuazonic Acid; C005197:alternariol; C018206:alternariol monomethyl ether