title: [Ecto-nucleotidases of ectonucleoside triphosphate diphosphohydrolase family: structure, localization and functional significance].
abstract: Ecto-nucleotidases are enzymes of hydrolase class. They split extracellular nucleoside tri- and diphosphate. In this review a short history of these enzymes investigation, classification, structure, and functional significance of ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDase) has been presented. These enzymes are glycoproteins anchored in membranes. They do not form phosphorylated enzyme's form during catalytic circle, and (by analogy with membrane-bound ATPases) form homooligomeric ensembles. Activity of these enzymes depends on bivalent ions, in particular Ca2+ and Mg2+. E-NTPDases function in the composition of ecto-nucleotidase cascade that contains other nucleotide-hydrolyzing enzymes. They regulate P2-receptors by hydrolyzing its ligand specifically ATP. Both modern information and results of our investigation about influence of different endo- and exogenous factors on activity of these enzymes has been presented.
authors.forename: S V
authors.initials: SV
authors.lastname: Iablons'ka
authors.forename: V K
authors.initials: VK
authors.lastname: Rybal'chenko
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D001081:Apyrase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology; D006801:Humans; D009708:Nucleotidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D015398:Signal Transduction; D013379:Substrate Specificity
chemical_list: D015703:Antigens, CD; D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D009708:Nucleotidases; D001081:Apyrase; C067869:CD39 antigen