title: Receptor tyrosine kinases and their activation in melanoma.
abstract: Receptor tyrosine kinases (RTKs) and their downstream signalling pathways have long been hypothesized to play key roles in melanoma development. A decade ago, evidence was derived largely from animal models, RTK expression studies and detection of activated RAS isoforms in a small fraction of melanomas. Predictions that overexpression of specific RTKs implied increased kinase activity and that some RTKs would show activating mutations in melanoma were largely untested. However, technological advances including rapid gene sequencing, siRNA methods and phospho-RTK arrays now give a more complete picture. Mutated forms of RTK genes including KIT, ERBB4, the EPH and FGFR families and others are known in melanoma. Additional over- or underexpressed RTKs and also protein tyrosine phosphatases (PTPs) have been reported, and activities measured. Complex interactions between RTKs and PTPs are implicated in the abnormal signalling driving aberrant growth and survival in malignant melanocytes, and indeed in normal melanocytic signalling including the response to ultraviolet radiation. Kinases are considered druggable targets, so characterization of global RTK activity in melanoma should assist the rational development of tyrosine kinase inhibitors for clinical use.
authors.affiliation: Department of Oncology, St James's Hospital, Dublin, Ireland  Division of Biomedical Sciences, St George's, University of London, London, UK. david.easty@ucd.ie
authors.forename: David J
authors.initials: DJ
authors.lastname: Easty
authors.forename: Steven G
authors.initials: SG
authors.lastname: Gray
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: O'Byrne
authors.forename: Dearbhaile
authors.initials: D
authors.lastname: O'Donnell
authors.forename: Dorothy C
authors.initials: DC
authors.lastname: Bennett
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008544:Melanocytes / Q000201:enzymology; D008545:Melanoma / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D009363:Neoplasm Proteins; D020794:Receptor Protein-Tyrosine Kinases; D017027:Protein Tyrosine Phosphatases