title: Metabolic analysis of rhubarb extract by rat intestinal bacteria using liquid chromatography-tandem mass spectrometry.
abstract: Through investigation of the metabolism of rhubarb extract by rat intestinal bacteria, a total of 14 components in rhubarb extract were found to be biotransformed. These components included aloe-emodin-O-glucosides, emodin-O-glucosides, chrysophanol-O-glucosides, physcion-O-glucosides and the corresponding aglycones. Rhein also could be biotransformed by rat intestinal bacteria. Twelve major metabolites were detected in the incubation sample. Under ESI tandem mass conditions, the sequential fragmentation patterns of [M H](-) ions were similar to those of free anthraquinones, thus allowing the rapid identification of the metabolites formed in incubation samples. The results suggested that the proposed hydrolysis of glycoside group followed by hydrogenation in quinoid moiety and/or further acetylation was the major biotransformation pathway for these anthraquinone glycosides by rat intestinal bacteria.
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, No. 24 Tongjia Lane, Nanjing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Song
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Fengguo
authors.initials: F
authors.lastname: Xu
authors.forename: Haijuan
authors.initials: H
authors.lastname: Dong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Zunjian
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D002853:Chromatography, Liquid / Q000379:methods*; D005243:Feces / Q000382:microbiology; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D007422:Intestines / Q000382:microbiology*; D008297:Male; D010936:Plant Extracts / Q000032:analysis* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000880:Anthraquinones; D006027:Glycosides; D010936:Plant Extracts