title: Genomic analysis of a pneumovirus isolated from dogs with acute respiratory disease.
abstract: A previously unrecognized virus belonging to the subfamily Pneumovirinae and most closely related to murine pneumovirus (MPV) was identified in domestic dogs in 2 related animal shelters. Additional diagnostic testing yielded 3 new viral isolates and identified 6 additional PCR positive dogs from other USA locations indicating that its distribution is not geographically limited. Nucleotide sequences encompassing 9 of the 10 genes were compared to the only 2 available MPV strains, 15 and J3666. Several features distinguished the canine pneumovirus (CnPnV) from the murine strains. Two regions of diversity were identified in the amino-proximal region of P and the overlapping P2 ORF was only 54 amino acids (aa) compared to 137aa in MPV. The G protein had an amino-terminal cytoplasmic tail 18aa longer than in the MPV strains. The CnPnV SH protein showed the highest divergence with only 90.2% aa identity when compared to MPV strain 15. Like strain 15, the CnPnV SH ORF coded for a protein of 92aa while J3666 has a 114aa variant. Comparison of CnPnV isolates at culture passages 4 and 17 revealed 7nt differences within the 8598nt sequenced. Of note was a substitution at nt 364 in G resulting in a termination codon that would produce a truncated G protein of 122aa. Analysis of early passage and ex vivo samples showed the termination codon in G to be predominant after 6 days in culture indicating rapid selection of the mutation in A72 cells.
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, Animal Health Diagnostic Center, Cornell University, Ithaca, NY 14853, USA. rwr3@cornell.edu
authors.forename: Randall
authors.initials: R
authors.lastname: Renshaw
authors.forename: Melissa
authors.initials: M
authors.lastname: Laverack
authors.forename: Nancy
authors.initials: N
authors.lastname: Zylich
authors.forename: Amy
authors.initials: A
authors.lastname: Glaser
authors.forename: Edward
authors.initials: E
authors.lastname: Dubovi
mesh_terms: D000818:Animals; D002460:Cell Line; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics; D016366:Open Reading Frames; D010802:Phylogeny; D018111:Pneumovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018186:Pneumovirus Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D014481:United States; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins