title: [Beta-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium].
abstract: Beta-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low beta-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that beta-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.
authors.affiliation: Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá DC, Colombia. lilian.chuaire@urosario.edu.co
authors.forename: Lilian
authors.initials: L
authors.lastname: Chuaire-Noack
authors.forename: Sandra
authors.initials: S
authors.lastname: Rondón-Lagos
authors.forename: Magda
authors.initials: M
authors.lastname: Sánchez-Corredor
authors.forename: Milcíades
authors.initials: M
authors.lastname: Ibáñez-Pinilla
authors.forename: Sandra
authors.initials: S
authors.lastname: Ramírez-Clavijo
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D016922:Cellular Senescence; D004847:Epithelial Cells / Q000201:enzymology* / Q000502:physiology; D005260:Female; D006801:Humans; D008875:Middle Aged; D010053:Ovary / Q000166:cytology*; D055815:Young Adult; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase