title: TRPV1, NK1 receptor and substance P immunoreactivity and gene expression in the rat lumbosacral spinal cord and urinary bladder after systemic, low dose vanilloid administration.
abstract: Transient receptor potential vanilloid 1 (TRPV1), neurokinin 1 (NK1) receptor and substance P (SP) immunoreactivity (-ir) and mRNA in the rat lumbosacral spinal cord and urinary bladder were measured 24h after s.c. injection of the vanilloids, capsaicin (50mg/kg) and resiniferatoxin (RTX, 100μg/kg), or vehicle (10% ethanol/10% Tween 80/saline). In the spinal cord, capsaicin significantly reduced TRPV1 and SP-ir (40-45%) in laminae I/II compared to controls, while RTX produced decreases of ~35%. NK1-ir in the spinal cord was unaffected by both vanilloid treatments. In the bladder, SP-ir was reduced in urothelial cells of some capsaicin- and RTX-treated rats, while SP-ir in the suburothelium and muscularis was significantly reduced by RTX. A significant increase in NK1-ir was observed in the urothelium and muscularis after capsaicin administration. Capsaicin significantly increased SP mRNA in the spinal cord, and TRPV1 and SP mRNA in the bladder, whereas RTX increased TRPV1, SP and NK1 mRNA in the spinal cord, and TRPV1 and SP mRNA in the bladder. These data suggest that stimulation of TRPV1 by low dose vanilloid administration can rapidly (within 24h) alter both transcription and translation of TRPV1 channels, SP and NK1 receptors in the rat urinary bladder and spinal cord.
authors.affiliation: School of Human Life Sciences, University of Tasmania, Launceston 7250, Tasmania, Australia.
authors.forename: Yujing J
authors.initials: YJ
authors.lastname: Heng
authors.forename: Cassandra I M
authors.initials: CI
authors.lastname: Saunders
authors.forename: Dale A
authors.initials: DA
authors.lastname: Kunde
authors.forename: Dominic P
authors.initials: DP
authors.lastname: Geraghty
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000008:administration & dosage* / Q000494:pharmacology; D004224:Diterpenes / Q000008:administration & dosage* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008159:Lumbar Vertebrae / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012447:Sacrum / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism*; D013373:Substance P / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*
chemical_list: D004224:Diterpenes; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; D013373:Substance P; C024353:resiniferatoxin; D002211:Capsaicin