title: Protective effects of methoxyflavone derivatives from black galingale against glutamate induced neurotoxicity in primary cultured rat cortical cells.
abstract: To examine the neuroprotective effects of black galingale, its protection was tested against glutamate-induced neurotoxicity in primary cortical cultured neurons. It was found that an aqueous extract of this medicinal plant exhibited significant protection against glutamate-induced toxicity in primary cultured rat cortical cells. In order to clarify the neuroprotective mechanism(s) of this observed effect, isolation was performed to seek and identify active fractions and components. By such fractionation, bioactive methoxyflavone derivatives were isolated from the methanol extracts from the air-dried rhizomes of black galingale. 5-Hydroxy-3,7,3',4'-tetramethoxyflavone exhibited significant neuroprotective activities against glutamate-induced toxicity, exhibiting cell viability of about 60-70%, at concentrations ranging from 0.1 μm to 10 μm. Therefore, the neuroprotective effect of black galingale might be due to the inhibition of glutamate-induced toxicity by the methoxyflavone derivatives it contains.
authors.affiliation: College of Natural Resources and Life Science, Dong-A University, Busan, South Korea.
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Moon
authors.forename: Sang-Buem
authors.initials: SB
authors.lastname: Cho
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Jun-Hyeong
authors.initials: JH
authors.lastname: Lee
authors.forename: Chi-Ho
authors.initials: CH
authors.lastname: Lee
authors.forename: Soo-Ki
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000473:pathology; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000209:etiology / Q000517:prevention & control*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027343:Rhizome / Q000737:chemistry; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D047309:Flavones; D018696:Neuroprotective Agents; D010936:Plant Extracts; D018698:Glutamic Acid