title: Clinical and antimicrobial efficacy of NitrAdine ™-based disinfecting cleaning tablets in complete denture wearers.
abstract: OBJECTIVE
  This study evaluated the efficacy of NitrAdine ™-based disinfecting cleaning tablets for complete denture, in terms of denture biofilm removal and antimicrobial action.
  
  
  METHODS
  Forty complete denture wearers (14 men and 26 women) with a mean age of 62.3 ± 9.0 years were randomly assigned to two groups and were instructed to clean their dentures according to two methods: brushing (control) - 3 times a day with denture brush and tap water following meals; brushing and immersion (Experimental) - brushing the denture 3 times a day with denture brush and tap water following meals and immersion of the denture in NitrAdine ™-based denture tablets (Medical Interporous ™). Each method was used for 21 days. Denture biofilm was disclosed by a 1% neutral red solution and quantified by means of digital photos taken from the internal surface before and after the use of the product. Microbiological assessment was conducted to quantify Candida sp.
  
  
  RESULTS
  An independent t-test revealed a significant lower biofilm percentage for the experimental group (4.7, 95% CI 2.4 to 7.9) in comparison with the control group (mean 37.5, 95% CI 28.2 to 48.1) (t38=7.996, p<0.001). A significant reduction of yeast colony forming units could be found after treatment with Medical Interporous ™ denture tablets as compared to the control group (Mann-Whitney test, Z=1.90; p<0.05).
  
  
  CONCLUSIONS
  The present findings suggest that NitrAdine ™-based disinfecting cleaning tablets are efficient in removal of denture biofilm. In addition, a clear antimicrobial action was demonstrated. Therefore, they should be recommended as a routine denture maintenance method for the prevention of the development of microbial biofilm induced denture stomatitis.
authors.affiliation: Department of Dental Materials and Prosthodontics, Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, SP, Brazil. chl@forp.usp.br
authors.forename: Cláudia Helena
authors.initials: CH
authors.lastname: Silva-Lovato
authors.forename: Bart de
authors.initials: Bd
authors.lastname: Wever
authors.forename: Els
authors.initials: E
authors.lastname: Adriaens
authors.forename: Helena de Freitas Oliveira
authors.initials: Hde F
authors.lastname: Paranhos
authors.forename: Evandro
authors.initials: E
authors.lastname: Watanabe
authors.forename: Maria Xavier
authors.initials: MX
authors.lastname: Pisani
authors.forename: Rafael Freitas de
authors.initials: RF
authors.lastname: Souza
authors.forename: Isabel Yoko
authors.initials: IY
authors.lastname: Ito
mesh_terms: D000368:Aged; D018441:Biofilms / Q000187:drug effects*; D003820:Denture Cleansers / Q000494:pharmacology*; D003824:Denture, Complete / Q000009:adverse effects / Q000382:microbiology*; D004202:Disinfectants / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009910:Oral Hygiene / Q000379:methods; D011897:Random Allocation; D013282:Stomatitis, Denture / Q000517:prevention & control; D013607:Tablets; D013997:Time Factors
chemical_list: D003820:Denture Cleansers; D004202:Disinfectants; D013607:Tablets