title: Severe stunting in blackgram caused by the Mungbean yellow mosaic virus (MYMV) KA27 DNA B component is ameliorated by co-infection or post-infection with the KA22 DNA B: MYMV nuclear shuttle protein is the symptom determinant.
abstract: Mungbean yellow mosaic virus-[India:Vigna] (MYMV-[IN:Vig]), a blackgram isolate of MYMV, has five variable and infective DNA B components of which KA22 and KA27 DNA Bs share only 72% nucleotide sequence identity between them. Agroinoculation of blackgram with partial dimers of DNA A and KA27 DNA B caused severe stunting and an inordinate delay in flowering. Interestingly, co-agroinoculation of KA27+KA22 DNA B components along with DNA A ameliorated severe stunting, rescued from the delay in flowering and caused the appearance of yellow mosaic symptom characteristic of KA22 DNA B. Post-agroinoculation of KA27 DNA B-infected blackgram plants with KA22 DNA B also resulted in the amelioration from severe stunting and in the alleviation from the delay in flowering. Alleviation from KA27 DNA B-type of symptom by co-infection or post-infection with KA22 DNA B did not result in a corresponding reduction in KA27 DNA B levels. Swapping of KA27 DNA B with the nuclear shuttle protein gene (NSP) of KA22 DNA B abolished severe stunting and caused the appearance of mild yellow symptom, suggesting that the NSP is the major symptom determinant in MYMV DNA B.
authors.affiliation: Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India.
authors.forename: Nagrani
authors.initials: N
authors.lastname: Mahajan
authors.forename: Chidambaram
authors.initials: C
authors.lastname: Parameswari
authors.forename: Karuppannan
authors.initials: K
authors.lastname: Veluthambi
mesh_terms: D016960:Agrobacterium tumefaciens / Q000254:growth & development / Q000378:metabolism; D053839:Begomovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D007887:Fabaceae / Q000821:virology*; D016679:Genome, Viral; D009687:Nuclear Proteins / Q000235:genetics*; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D009687:Nuclear Proteins; D010940:Plant Proteins; D014764:Viral Proteins