title: Rapid and efficient isolation of high quality nucleic acids from plant tissues rich in polyphenols and polysaccharides.
abstract: Isolation of high quality nucleic acids from plant tissues rich in polysaccharides and polyphenols is often difficult. The presence of these substances can affect the quality and/or quantity of the nucleic acids isolated. Here, we describe a rapid and efficient nucleic acids extraction protocol that in contrast to other methods tested, effectively purify high quality nucleic acids from plant tissues rich in polysaccharides and polyphenolic compounds such as different grape tissues and fruit tissue of fruit trees. The nucleic acids isolated with this protocol were successfully used for many functional genomic based experiments including polymerase chain reaction, reverse transcription polymerase chain reaction (RT-PCR), cloning, and semiquantitative RT-PCR.
authors.affiliation: Department of Agricultural Biotechnology, Faculty of Engineering and Technology, Imam Khomeini International University, P.O. Box 34149-288, Qazvin, Islamic Republic of Iran.
authors.forename: Reza Heidari
authors.initials: RH
authors.lastname: Japelaghi
authors.forename: Raheem
authors.initials: R
authors.lastname: Haddad
authors.forename: Ghasem-Ali
authors.initials: GA
authors.lastname: Garoosi
mesh_terms: D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005638:Fruit / Q000737:chemistry*; D028521:Musa / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D059808:Polyphenols; D011134:Polysaccharides; D018749:RNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027824:Rosaceae / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry
chemical_list: D018744:DNA, Plant; D059808:Polyphenols; D011134:Polysaccharides; D018749:RNA, Plant