title: Comparison of metabolic profiling of cyanidin-3-O-galactoside and extracts from blueberry in aged mice.
abstract: The metabonomics changes of plasma and brain tissue after dietary supplementation with blueberry extracts (BBE) and cyanidin-3-O-galactoside from blueberry (BBM) in aged mice were investigated by (1)H NMR technique. The mice received intragastric administration of BBE (200 mg/kg/day), BBM (50 mg/kg/day), and saline water (0.9%) for 6 weeks, respectively, in the BBE, BBM, and control groups. At the end of the experiment, plasma and brain samples were collected for NMR analysis. The results demonstrated that the level of choline in plasma from BBE and BBM groups were obviously elevated relative to the control group, whereas the levels of lactate and phosphocholine in plasma were remarkably reduced. Compared with those in the control group, the levels of choline and GABA in the brain from the BBE group were obviously increased, whereas glutamate and phosphocholine in the BBE group were significantly decreased. The level of taurine in the brain from the BBM group was particularly higher than that in the control group. These results indicated supplementation with BBE or BBM might induce similar changes of endogenous plasma and brain metabolic profiles in aged mice.
authors.affiliation: Department of Nutrition and Food Science, Institute of Health and Environmental Medicine of Academy of Military Medical Sciences , 1 Dali Road, Tianjin 300050, China.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.forename: Daomei
authors.initials: D
authors.lastname: Cheng
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Yan
authors.forename: Yiyong
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yugang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000872:Anthocyanins / Q000378:metabolism / Q000493:pharmacokinetics*; D029798:Blueberry Plants / Q000737:chemistry*; D001923:Brain Chemistry; D002794:Choline / Q000032:analysis / Q000097:blood; D005638:Fruit / Q000737:chemistry*; D005697:Galactosides / Q000378:metabolism / Q000493:pharmacokinetics*; D018698:Glutamic Acid / Q000032:analysis; D019344:Lactic Acid / Q000097:blood; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics / Q000379:methods; D051379:Mice; D010767:Phosphorylcholine / Q000032:analysis / Q000097:blood; D010936:Plant Extracts / Q000378:metabolism / Q000493:pharmacokinetics*; D013654:Taurine / Q000031:analogs & derivatives / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis
chemical_list: D000872:Anthocyanins; D005697:Galactosides; D010936:Plant Extracts; D010767:Phosphorylcholine; D013654:Taurine; D019344:Lactic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; C546035:cyanidin 3-galactoside; D002794:Choline; C111425:taurineamide