title: Proinflammatory fecal mRNA and childhood bacterial enteric infections.
abstract: INTRODUCTION: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. RESULTS: Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. DISCUSSION: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. METHODS: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL-1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.
authors.affiliation: Washington University School of Medicine; Department of Pediatrics; Division of Pediatric Gastroenterology and Nutrition; St. Louis, MO USA.
authors.forename: William E
authors.initials: WE
authors.lastname: Bennett
authors.forename: Rosbel
authors.initials: R
authors.lastname: González-Rivera
authors.forename: Bao N
authors.initials: BN
authors.lastname: Puente
authors.forename: Nurmohammad
authors.initials: N
authors.lastname: Shaikh
authors.forename: Harold J
authors.initials: HJ
authors.lastname: Stevens
authors.forename: Jody C
authors.initials: JC
authors.lastname: Mooney
authors.forename: Eileen J
authors.initials: EJ
authors.lastname: Klein
authors.forename: Donna M
authors.initials: DM
authors.lastname: Denno
authors.forename: Andrew
authors.initials: A
authors.lastname: Draghi
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Sylvester
authors.forename: Phillip I
authors.initials: PI
authors.lastname: Tarr