title: Long-term cultured human skin mast cells are suitable for pharmacological studies of anti-allergic drugs due to high responsiveness to FcεRI cross-linking.
abstract: Human skin mast cells proliferated in the presence of interleukin (IL)-4+SCF (expanding 18-fold in 8 weeks) and acquired profound responsiveness towards high affinity IgE receptor (FcεRI) cross-linking, liberating about 75% of their histamine. In a proof-of-concept, we found that these cells are useful for pharmacological testing. Even a subtle inhibition of degranulation can be visualized. This model might prove valuable in tests of novel anti-allergic drugs.
authors.affiliation: Department of Dermatology and Allergy, Charité Campus Mitte, Universitätsmedizin Berlin, Berlin, Germany. sven.guhl@charite.de
authors.forename: Sven
authors.initials: S
authors.lastname: Guhl
authors.forename: Metin
authors.initials: M
authors.lastname: Artuc
authors.forename: Angelos
authors.initials: A
authors.lastname: Neou
authors.forename: Magda
authors.initials: M
authors.lastname: Babina
authors.forename: Torsten
authors.initials: T
authors.lastname: Zuberbier
mesh_terms: D018926:Anti-Allergic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D017455:Receptors, IgE / Q000737:chemistry* / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D019089:Stem Cell Factor / Q000494:pharmacology; D013997:Time Factors
chemical_list: D018926:Anti-Allergic Agents; D017455:Receptors, IgE; D019089:Stem Cell Factor; D015847:Interleukin-4