title: The impact of early viral infections and graft-versus-host disease on immune reconstitution following paediatric stem cell transplantation.
abstract: Viral infections and graft-versus-host disease (GVHD) render an impact on both the clinical and immunological recovery following allogeneic hematopoietic stem cell transplantation (HSCT). We studied the recuperation of the immune defence after transplant in the paediatric setting and assessed the impact of early (<100 days post-HSCT) viral [cytomegalovirus (CMV), Ebstein-Barr virus (EBV) and adenovirus] reactivations/infections and GVHD. Fifty-one paediatric recipients of HSCT were enrolled. T cell recovery was evaluated on lymphocyte subpopulations using flow cytometry and functionally by measuring T cell excision circles (TRECs) and through the analysis of T lymphocyte responses to mitogens. B cell recovery was studied by flow cytometry and functionally by ELISPOT. Acute and mild chronic GVHD allowed for a brisk recovery of both cellular and humoral immunity while moderate to severe chronic graft-versus-host disease (cGVHD) associated with a significant, tampering effect on the immunological recovery after transplant. In the former group, the early viral reactivations/infections seemingly linked with a delayed recovery of T lymphocytes and low TRECs values. Moderate to severe cGVHD appears to associate with an impaired immunological recovery after HSCT. Early viral infections linked with prolonged T cell immunodeficiency and thymic dysfunction may be indicative of the presence of subclinical GVHD.
authors.affiliation: Children's Hospital, Division of Hematology-Oncology and Stem Cell Transplantation, Helsinki University Central Hospital, Helsinki, Finland. helena.olkinuora@hus.fi
authors.forename: H
authors.initials: H
authors.lastname: Olkinuora
authors.forename: E
authors.initials: E
authors.lastname: von Willebrand
authors.forename: J M
authors.initials: JM
authors.lastname: Kantele
authors.forename: O
authors.initials: O
authors.lastname: Vainio
authors.forename: K
authors.initials: K
authors.lastname: Talvensaari
authors.forename: U
authors.initials: U
authors.lastname: Saarinen-Pihkala
authors.forename: S
authors.initials: S
authors.lastname: Siitonen
authors.forename: K
authors.initials: K
authors.lastname: Vettenranta
mesh_terms: D000256:Adenoviridae / Q000276:immunology; D000293:Adolescent; D001402:B-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000276:immunology; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007223:Infant; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*; D014777:Virus Diseases / Q000276:immunology*; D055815:Young Adult