title: Detecting protein-protein interactions in vesicular stomatitis virus using a cytoplasmic yeast two hybrid system.
abstract: Protein-protein interactions play an important role in many virus-encoded functions and in virus-host interactions. While a "classical" yeast two-hybrid system (Y2H) is one of the most common techniques to detect such interactions, it has a number of limitations, including a requirement for the proteins of interest to be relocated to the nucleus. Modified Y2H, such as the Sos recruitment system (SRS), which detect interactions occurring in the cytoplasm rather than the nucleus, allow proteins from viruses replicating in the cytoplasm to be tested in a more natural context. In this study, a SRS was used to detect interactions involving proteins from vesicular stomatitis virus (VSV), a prototypic non-segmented negative strand RNA (NNS) virus. All five full-length VSV proteins, as well as several truncated proteins, were screened against each other. Using the SRS, most interactions demonstrated previously involving VSV phosphoprotein, nucleocapsid (N) and large polymerase proteins were confirmed independently, while difficulties were encountered using the membrane associated matrix and glycoproteins. A human cDNA library was also screened against VSV N protein and one cellular protein, SFRS18, was identified which interacted with N in this context. The system presented can be redesigned easily for studies in other less tractable NNS viruses.
authors.affiliation: Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
authors.forename: Megan
authors.initials: M
authors.lastname: Moerdyk-Schauwecker
authors.forename: Darla
authors.initials: D
authors.lastname: Destephanis
authors.forename: Eric
authors.initials: E
authors.lastname: Hastie
authors.forename: Valery Z
authors.initials: VZ
authors.lastname: Grdzelishvili
mesh_terms: D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques; D018116:Vesiculovirus / Q000502:physiology*; D015678:Viral Structural Proteins / Q000378:metabolism*
chemical_list: D015678:Viral Structural Proteins