title: Identification of alternatively activated macrophages in new-onset paediatric and adult immunoglobulin A nephropathy: potential role in mesangial matrix expansion.
abstract: OBJECTIVE
  New onset of the clinical symptoms of immunoglobulin A (IgA) nephropathy (IgAN) manifests with proliferative glomerular lesions in children, whereas adults exhibit mesangial matrix expansion and interstitial fibrosis. Alternatively, activated (M2) macrophages have been implicated in promoting tissue fibrosis in some settings. Therefore, the aim of this study was to investigate whether M2 macrophages are present in new-onset IgAN and if they are related to pathological differences between paediatric and adult disease.
  
  
  RESULTS
  Biopsy specimens from paediatric (<10 years, n=14; >12 years, n=15) and adult (n=27) IgAN showed a significant infiltrate of CD68(+) macrophages. M2 macrophages, identified by CD163 or CD204 expression, were detected in glomeruli and the interstitium, being more prominent in adults versus young children. CD163(+) and CD204(+) macrophages were present in areas of fibrosis containing myofibroblasts, and double staining showed that CD163(+) cells produced the profibrotic molecule, connective tissue growth factor. In young children, total CD68(+) macrophages, but not M2 macrophages, correlated with glomerular hypercellularity. In contrast, in adults and older children, mesangial matrix expansion correlated with M2 macrophages but not with the total CD68(+) macrophage infiltrate.
  
  
  CONCLUSIONS
  Alternatively activated M2 macrophages are present in new-onset paediatric and adult IgAN, and this population may promote the development of fibrotic lesions.
authors.affiliation: Department of Pediatrics, Niigata University Medical and Dental Hospital, Niigata 951-8510 Japan. ikezumi@med.niigata-u.ac.jp
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ikezumi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tamaki
authors.initials: T
authors.lastname: Karasawa
authors.forename: Hiroya
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Naofumi
authors.initials: N
authors.lastname: Imai
authors.forename: Ichiei
authors.initials: I
authors.lastname: Narita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawachi
authors.forename: Kevan R
authors.initials: KR
authors.lastname: Polkinghorne
authors.forename: David J
authors.initials: DJ
authors.lastname: Nikolic-Paterson
authors.forename: Makoto
authors.initials: M
authors.lastname: Uchiyama
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005355:Fibrosis / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005920:Glomerular Mesangium / Q000473:pathology*; D005922:Glomerulonephritis, IGA / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008262:Macrophage Activation; D008264:Macrophages / Q000473:pathology*; D008297:Male; D055815:Young Adult