title: Immunolocalization and activity of the MMP-9 and MMP-2 in odontogenic region of the rat incisor tooth after post shortening procedure.
abstract: MMP-9 and MMP-2 are metalloproteinases which degrade the denatured collagen fibers. However, there is no report about roles of these MMPs in the odontogenic region of the adult rat incisor tooth under different eruption conditions. Male Wistar rats were divided in a normofunctional group (NF) in which their lower teeth remained in a normal eruption. In a hypofunctional group (HP) rats underwent shortening of their lower left incisor tooth every 2 days during 12 days. The eruption rate as well as the expression and activities of MMP-9 and MMP-2 were evaluated using imunohistochemistry and zymography. Although the shortening increased the eruption rate, no changes in the MMP-9 and MMP-2 were observed. We conclude that in adult rats, in opposite to development of tooth, the MMP-9 and MMP-2 present in the odontogenic region does not seem to play a direct role in the remodeling matrix, even after post-shortening procedures which to lead an acceleration of the eruption process in the incisor.
authors.affiliation: Departamento de Biologia Estrutural Molecular e Genética, UEPG, Avenue Carlos Cavalcanti, 4748, Ponta Grossa, PR, 84030-900, Brazil. osteoblasto@ig.com.br
authors.forename: Jose Rosa
authors.initials: JR
authors.lastname: Gomes
authors.forename: Nádia Fayez
authors.initials: NF
authors.lastname: Omar
authors.forename: Juliana
authors.initials: J
authors.lastname: dos Santos Neves
authors.forename: Eliene Aparecida Orsini
authors.initials: EA
authors.lastname: Narvaes
authors.forename: Pedro Duarte
authors.initials: PD
authors.lastname: Novaes
mesh_terms: D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D003771:Dental Papilla / Q000166:cytology / Q000378:metabolism; D003795:Dental Sac / Q000166:cytology / Q000378:metabolism; D057075:Enzyme Assays; D007180:Incisor / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009804:Odontoblasts / Q000378:metabolism; D009805:Odontogenesis; D051381:Rats; D017208:Rats, Wistar; D014078:Tooth Eruption
chemical_list: D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat; D020780:Matrix Metalloproteinase 9