title: Effect of Scrophularia buergeriana extract on the degranulation of mast cells and ear swelling induced by dinitrofluorobenzene in mice.
abstract: Scrophularia buergeriana Miquel (Scrophulariaceae, SB) is a biennial plant native to Korea, northern China, and Japan that plays an important role in traditional medicine. The dried root of SB has long been used in oriental medicine for treatment of fever, swelling, constipation, pharyngitis, neuritis, and laryngitis. In the present study, we evaluated the ethanol extract of SB (SBE) to determine if it exerted any anti-allergic effects that had not previously been demonstrated. SBE markedly inhibited β-hexosaminidase and histamine release and suppressed the expression of tumor necrosis factor-α and interleukin-4 cytokines by RBL-2H3 mast cells. In addition, topical treatment with SBE effectively reduced allergic inflammation in a dinitrofluorobenzene-induced contact hypersensitivity mouse model. These results strongly suggest that SBE is a promising source of anti-allergic agents.
authors.affiliation: Department of Biomedical Science, Catholic University of Daegu, Gyeongsan-Si, 712-702, South Korea. toto0818@cu.ac.kr
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Kim
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Hee Hwan
authors.initials: HH
authors.lastname: Lee
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Kyung Woo
authors.initials: KW
authors.lastname: Park
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D045744:Cell Line, Tumor; D003877:Dermatitis, Contact / Q000188:drug therapy*; D004139:Dinitrofluorobenzene; D004423:Ear; D004487:Edema / Q000188:drug therapy*; D006636:Histamine Release; D007249:Inflammation / Q000188:drug therapy*; D015847:Interleukin-4 / Q000096:biosynthesis; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008518:Medicine, East Asian Traditional; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D032266:Scrophularia; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D004139:Dinitrofluorobenzene; D001619:beta-N-Acetylhexosaminidases