title: Protective effects of sesame oil on 4-NQO-induced oxidative DNA damage and lipid peroxidation in rats.
abstract: Sesame oil could be considered as a potent antioxidant and dietary supplement. It possesses antimutagenic, anti-inflammatory and anti-cardiac toxicity. In the view of available findings, the current study focused on determining the protective effects of sesame oil on 4-Nitroquinoline-1-oxide (4-NQO) -induced oxidative DNA damage and lipid peroxidation (LPO) in rats. Seven groups of Wistar albino rats each with 6 either sex were used. Groups were given vehicle control and sesame oil alone orally and 4-NQO (30 mg/kg) by intraperitoneal injection. Following the four dose levels (1, 2, 4, and 8 ml/kg orally), sesame oil plus 4-NQO were also tested. After 24 hours of 4-NQO injection, blood samples were drawn by venipuncture. DNA damage (8-hydroxy-2-deoxy guanosine; 8-OHdG) and LPO were estimated. LPO from the 4-NQO-treated group was 2.5-fold higher than that of the control LPO. Pretreatment with sesame oil reduced this by 16-61%. 8-OHdG DNA damage from 4-NQO was found to be 3-fold higher than that of controls. Pretreatment with sesame oil effectively protected against DNA damage in a dose-dependent fashion. This study indicates that the antioxidant, sesame oil, effectively protected DNA damage and LPO induced by 4-NQO.
authors.affiliation: Center for Research & Development, PRIST University, Thanjavur, India. arumugamgenetics@yahoo.co.in
authors.forename: Ponnan
authors.initials: P
authors.lastname: Arumugam
authors.forename: Samiraj
authors.initials: S
authors.lastname: Ramesh
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012715:Sesame Oil / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D015112:4-Nitroquinoline-1-oxide; D012715:Sesame Oil