title: Determination of dimethyl fumarate in desiccant and antimould sachets by reversed-phase liquid chromatography.
abstract: A method for the determination of dimethyl fumarate (DMF) in desiccant and antimould sachets, employed for protecting consumer products from humidity and mould, has been developed. The method is based on a solid-liquid extraction followed by HPLC-UV analysis. The method was validated with respect to recovery, linearity, limits of detection and quantitation and precision. The recovery was 98%. The correlation coefficient value (r) was equal to 0.94. Both intra- and inter-day precisions were studied at several concentration levels, being satisfactory in all cases (RSD < 5). Limits of detection and quantification values were in the low microgram per gram level, thus allowing the determination of DMF at concentrations below the limit established (0.1 mg/kg) by the recent EU Directive (Decision 2009/251/EC). The proposed procedure was applied for the determination of the target compound in 41 desiccant and antimould samples. DMF was detected in 39.0% of samples and its content in many samples exceeded the legal limits. The results of our analysis highlight the high risk of exposure to this powerful allergic sensitizer for consumers.
authors.affiliation: Dipartimento di Chimica Farmaceutica e Tossicologica, Università degli Studi di Napoli Federico II, Via D. Montesano, 49, I-80131, Naples, Italy.
authors.forename: Oriella
authors.initials: O
authors.lastname: Gennari
authors.forename: Domenico
authors.initials: D
authors.lastname: Montesano
authors.forename: Antonio
authors.initials: A
authors.lastname: Salzano
authors.forename: Stefania
authors.initials: S
authors.lastname: Albrizio
authors.forename: Lucia
authors.initials: L
authors.lastname: Grumetto
mesh_terms: D000935:Antifungal Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D000069462:Dimethyl Fumarate; D005650:Fumarates / Q000032:analysis* / Q000302:isolation & purification; D058427:Hygroscopic Agents / Q000032:analysis* / Q000302:isolation & purification; D019064:Product Packaging / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D000935:Antifungal Agents; D005650:Fumarates; D058427:Hygroscopic Agents; D000069462:Dimethyl Fumarate