title: Genetic variants of Anaplasma phagocytophilum in wild caprine and cervid ungulates from the Alps in Tyrol, Austria.
abstract: The occurrence of genetic variants of Anaplasma phagocytophilum was studied in wild ungulates from the northern and central eastern Alps in Tyrol, Austria. For this purpose, spleen samples collected from 53 game animals during the hunting season 2008/2009 (16 roe deer [Capreolus capreolus], 10 red deer [Cervus elaphus], 16 Alpine chamois [Rupicapra r. rupicapra], 7 Alpine ibex [Capra i. ibex], and 4 European mouflons [Ovis orientalis musimon]) were analyzed. Thirty-five animals originated from the Karwendel mountains, 12 from the Kaunertal area (Ötztal Alps), and the remaining from other mountainous areas in Tyrol. DNA extracts were screened with a real-time polymerase chain reaction targeting the msp2 gene of A. phagocytophilum. A total of 23 (43.4%) samples, from all ungulate species studied, were A. phagocytophilum positive. As of the date of this article, A. phagocytophilum has not been reported in the Alpine ibex. The positive samples were investigated further with polymerase chain reactions for amplification of the partial 16S rRNA, groEL, and msp4 genes. Sequence analysis using forward and reverse primers revealed seven different 16S rRNA gene variants. No variant could be attributed to any particular ungulate species. The groEL gene revealed 11 different variants, which grouped in the phylogenetic analysis into two distinct clusters: one cluster contained the sequences from roe deer, whereas the sequences of the other species formed the second cluster. The msp4 gene showed a high degree of variability in the amplified part with a total of 10 different sequence types. The results show that the wild mountain ungulates were infected to a considerable extent with various variants of A. phagocytophilum. The pathogenicity of the variants and the reservoir competence of the species investigated in this study deserve further attention in future studies.
authors.affiliation: Faculty of Veterinary Medicine and Parasitology, Institute of Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-University, Leopoldstrasse 5, Munich, Germany. cornelia.silaghi@tropa.vetmed.uni-muenchen.de
authors.forename: Cornelia
authors.initials: C
authors.lastname: Silaghi
authors.forename: Dietmar
authors.initials: D
authors.lastname: Hamel
authors.forename: Claudia
authors.initials: C
authors.lastname: Thiel
authors.forename: Kurt
authors.initials: K
authors.lastname: Pfister
authors.forename: Lygia Maria Friche
authors.initials: LM
authors.lastname: Passos
authors.forename: Steffen
authors.initials: S
authors.lastname: Rehbein
mesh_terms: D041081:Anaplasma phagocytophilum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D001317:Austria / Q000453:epidemiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012418:Ruminants / Q000382:microbiology*; D017421:Sequence Analysis; D013154:Spleen / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60; D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; C488283:msp2 protein, Anaplasma phagocytophilum