title: The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti.
abstract: Pyridoxal 4-dehydrogenase catalyzes the irreversible oxidation of pyridoxal to 4-pyridoxolactone and is involved in degradation pathway I of pyridoxine, a vitamin B(6) compound. Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD(+)-bound enzyme.
authors.affiliation: Faculty of Agriculture and Agricultural Science Program, Graduate School of Integral Arts and Science, Kochi University, Nankoku, Kochi, Japan.
authors.forename: Huy Nhat
authors.initials: HN
authors.lastname: Chu
authors.forename: Jun
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yagi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D020560:Proteobacteria / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C042642:pyridoxal dehydrogenase; C121603:1-phenylethanol dehydrogenase