title: Specific phage-displayed peptides discriminate different forms of neurocysticercosis by antibody detection in the serum samples.
abstract: Neurocysticercosis (NC), caused by Taenia solium metacestode, infects the central nervous system and is a devastating parasitic infection. Diagnosis is based on symptoms, imaging, serology and epidemiology. Current markers present variable sensitivity and specificity, frequent cross-reactions and are not able to discriminate NC clinical forms. The aim of this study was to select mimotopes of T. solium metacestode antigens that may be used in NC immunodiagnosis, specifically to discriminate between active and inactive forms. A random peptide phage display library was screened against IgY from chickens immunized with total saline extract from T. solium metacestodes and validated against 110 serum samples, classified into active NC (18), inactive NC (22), cross-reactive parasitic diseases (40) and healthy controls (30). We have successfully selected seven peptides with significant immunoreactivity to IgG of NC patients, with sensitivity ranging from 95.5% to 100% to detect the inactive form and specificity varied from 85.7% to 94.3%. One phage-displayed peptide (Cc48) can be directly used as biomarker to distinguish inactive from active forms with an accuracy of 95.7%, and this novel mimotope may also be used as an auxiliary tool to neuroimaging tests and treatment follow-up.
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: M N
authors.initials: MN
authors.lastname: Manhani
authors.forename: V S
authors.initials: VS
authors.lastname: Ribeiro
authors.forename: R
authors.initials: R
authors.lastname: Cardoso
authors.forename: C
authors.initials: C
authors.lastname: Ueira-Vieira
authors.forename: L R
authors.initials: LR
authors.lastname: Goulart
authors.forename: J M
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002645:Chickens; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology*; D010274:Parasitology / Q000379:methods*; D019151:Peptide Library; D010455:Peptides / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D044967:Serum / Q000737:chemistry; D041201:Taenia solium / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D019151:Peptide Library; D010455:Peptides