title: An evaluation of ultraviolet light (UV254) as a means to inactivate porcine reproductive and respiratory syndrome virus on common farm surfaces and materials.
abstract: A study was conducted to assess the effect of UV(254) on the concentration and viability of PRRSV on surfaces and materials commonly encountered on swine farms. A standard quantity (5 × 10(6)TCID(50), total dose) of a PRRSV modified live vaccine virus was inoculated onto 2 matched sets of surfaces/materials including wood, plastic, latex, rubber, styrofoam, metal, leather, cloth, concrete, cardboard, glass and paper. One set was exposed to UV(254) radiation (treatments) and the other to incandescent light (controls) for a 24h period. During this time, treatments and controls were swabbed at 10 min intervals from 0 to 60 min post-inoculation (PI) and again at 24h PI. The quantity of PRRSV RNA on each item at each sampling time was calculated by RT-PCR and the presence of viable PRRSV in each sample was determined by swine bioassay. A significant reduction (p<0.0001) in the quantity of PRRSV RNA was demonstrated at 24h PI independent of treatment. In addition, a significant reduction (p=0.012) in the number of UV(254)-treated surfaces which harbored viable virus was observed at 60 min (0/12 positive) when compared to control surfaces (5/12 positive). In addition, all UV(254) treated samples collected between 10 and 50 min PI were bioassay negative. These results suggest that UV(254) is an effective means to inactivate PRRSV on commonly encountered farm surfaces and materials and inactivation can be accomplished following 10 min of exposure.
authors.affiliation: Swine Disease Eradication Center, University of Minnesota College of Veterinary Medicine, 385c 1988 Fitch Avenue, St. Paul, MN 55108, USA. deexx004@umn.edu
authors.forename: Scott
authors.initials: S
authors.lastname: Dee
authors.forename: Satoshi
authors.initials: S
authors.lastname: Otake
authors.forename: John
authors.initials: J
authors.lastname: Deen
mesh_terms: D000383:Agriculture; D000818:Animals; D004203:Disinfection / Q000379:methods*; D006799:Housing, Animal; D019316:Porcine respiratory and reproductive syndrome virus / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D014466:Ultraviolet Rays; D038301:Virus Inactivation / Q000528:radiation effects*