title: Sequence analysis of a group of low molecular-weight plasmids carrying multiple IS903 elements flanking a kanamycin resistance aph gene in Salmonella enterica serovars.
abstract: A group of low molecular-weight ColE1-like plasmids carrying the aph sequence type aph(ii) from three different Salmonella serovars were sequenced. These plasmids carry two or more copies of IS903 elements, with up to 21bp sequence differences to one another, two of which flank the aph gene. This group of plasmids did not appear to carry any known mobilization genes and instead carry three open reading frames encoding hypothetical proteins of unknown function possibly organized in an operon. The plasmid replication region (RNA I/II--rom) of this plasmid group showed extensive homology to that of pKPN2 plasmid of Klebsiella pneumoniae and pCol-let plasmid of Escherichia coli. Three of the four plasmids had identical sequences, and the fourth had an extra copy of IS903 with target duplication, suggesting a recent divergence in the different Salmonella serovars from a common ancestor.
authors.affiliation: Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 E. Mermaid Ln., Wyndmoor, PA 19038, USA. chin-yi.chen@ars.usda.gov
authors.forename: Chin-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Terence P
authors.initials: TP
authors.lastname: Strobaugh
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Lindsey
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Frye
authors.forename: Gaylen
authors.initials: G
authors.lastname: Uhlich
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D023061:Gene Order; D005798:Genes, Bacterial; D007613:Kanamycin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D019779:Salmonella enterica / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial