title: Multimodal approaches of the hydroalcoholic extract of Diospyros peregrina fruits in diabetic therapy.
abstract: The matured fruits of Diospyros peregrina possess significant antidiabetic activity. The present investigation was undertaken to evaluate the target of action of the hydroalcoholic extract of D. peregrina fruit in diabetic pathophysiology. The extract was tested in vitro for inhibition of glucose absorption by α-amylase inhibition assay, glucose uptake study by isolated rat hemidiaphragm method and free radical scavenging activity by DPPH method. The extract exhibited significant α-amylase inhibition (IC₅₀ 136.5 µg mL⁻¹), peripheral glucose utilisation and radical scavenging (IC₅₀ 167.5 µg mL⁻¹) activity. From the results, it may be concluded that the hydroalcoholic extract of D. peregrina fruit can counteract diabetes by multiple mechanisms, namely inhibition of carbohydrate absorption, improving peripheral glucose utilisation and antioxidant defence.
authors.affiliation: Advance Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India. s.dewanjee@yahoo.com
authors.forename: Saikat
authors.initials: S
authors.lastname: Dewanjee
authors.forename: Moumita
authors.initials: M
authors.lastname: Gangopadhyay
authors.forename: Anup K
authors.initials: AK
authors.lastname: Das
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds; D001786:Blood Glucose / Q000187:drug effects; D003920:Diabetes Mellitus / Q000188:drug therapy*; D029788:Diospyros / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000627:therapeutic use*; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D001713:Biphenyl Compounds; D001786:Blood Glucose; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; D000516:alpha-Amylases; D005947:Glucose