title: Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor.
abstract: Here we show the molecular basis for the inhibition of peptidoglycan (PGN)-induced TLR2 signaling by a major green tea polyphenol epigallocatechin-3-gallate (EGCG). Recently, we identified the 67-kDa laminin receptor (67LR) as the cell-surface EGCG receptor. Anti-67LR antibody treatment or silencing of 67LR resulted in abrogation of the inhibitory action of EGCG on PGN-induced production of pro-inflammatory mediators and activation of mitogen-activated protein kinases. Silencing of Toll-interacting protein (Tollip), a negative regulator of TLR signaling impaired the TLR2 signaling inhibitory activity of EGCG, suggesting that TLR2 response could be inhibited by EGCG via 67LR and Tollip.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Eui-Hong
authors.initials: EH
authors.lastname: Byun
authors.forename: Toshinori
authors.initials: T
authors.lastname: Omura
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D018836:Inflammation Mediators / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D010457:Peptidoglycan / Q000494:pharmacology*; D010636:Phenols / Q000494:pharmacology; D059808:Polyphenols; D017474:Receptors, Laminin / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013662:Tea / Q000737:chemistry*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D018836:Inflammation Mediators; D047908:Intracellular Signaling Peptides and Proteins; D010457:Peptidoglycan; D010636:Phenols; D059808:Polyphenols; D017474:Receptors, Laminin; D013662:Tea; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; C485837:Tollip protein, mouse; D002392:Catechin; C045651:epigallocatechin gallate