title: Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning.
abstract: Many stem cells divide asymmetrically to balance self-renewal and differentiation. In Drosophila testes, two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs), cohere and regulate one another. Here, we report that CySCs divide asymmetrically through repositioning the mitotic spindle around anaphase. CySC spindle repositioning requires functional centrosomes, Dynein and the actin-membrane linker Moesin. Anaphase spindle repositioning is required to achieve high-fidelity asymmetric divisions in CySCs, thus maintaining both GSC and CySC numbers. We propose that dynamic spindle repositioning allows CySCs to divide asymmetrically while accommodating the structure of the GSCs they encapsulate.
authors.affiliation: Department of Biomedical Engineering, Center for Ultrafast Optical Science, 1101 Beal Ave., Ann Arbor, MI 48109, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Amita
authors.initials: A
authors.lastname: Tiyaboonchai
authors.forename: Yukiko M
authors.initials: YM
authors.lastname: Yamashita
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Hunt
mesh_terms: D000705:Anaphase; D000818:Animals; D002455:Cell Division; D018385:Centrosome; D004330:Drosophila; D004398:Dyneins; D005854:Germ Cells; D008297:Male; D008840:Microfilament Proteins; D008941:Spindle Apparatus / Q000378:metabolism* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*; D013737:Testis / Q000166:cytology*
chemical_list: D008840:Microfilament Proteins; C071058:moesin; D004398:Dyneins