title: Differentially expressed protein profile of renal tubule cell stimulated by elevated uric acid using SILAC coupled to LC-MS.
abstract: OBJECTIVE
  Hyperuricemia could lead to serious renal disease. It will advance our understanding of the mechanism of this disease to study the differentially expressed protein in renal tubular epithelial cells stimulated by elevated uric acid.
  
  
  METHODS
  We used SILAC coupled to LC-MS to study differentially expressed protein profile and to analysis the functional status of renal tubular epithelial cells stimulated by 600μM uric acid, and to investigate the potential biology function. The MS results were analyzed by online platform and further confirmed by western blotting.
  
  
  RESULTS
  789 differentially expressed proteins were identified, of which 42 proteins and 49 proteins were related with cell proliferation and cell apoptosis, respectively. Pathway analysis showed that MAPK signaling pathway was a key pathway related to the function of these proteins. In addition, prohibitin-2 was identified to be related to renal cell transdifferentiation stimulated by elevated uric acid.
  
  
  CONCLUSIONS
  This work provides an overview of protein expression changes in HK-2 cells treated with elevated uric acid and will contribute to further study of cell apoptosis and fibrosis in renal disease patients.
authors.affiliation: Key Kidney Department, Institute of Nephrology of Chinese PLA, General Hospital of Chinese PLA, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Quan
authors.forename: Xue
authors.initials: X
authors.lastname: Peng
authors.forename: Shuwen
authors.initials: S
authors.lastname: Liu
authors.forename: Fu
authors.initials: F
authors.lastname: Bo
authors.forename: Lv
authors.initials: L
authors.lastname: Yang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Huang
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
authors.forename: Wu
authors.initials: W
authors.lastname: Di
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation; D002853:Chromatography, Liquid; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D007684:Kidney Tubules / Q000166:cytology*; D013058:Mass Spectrometry; D000091402:Prohibitins; D020543:Proteome / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction; D014527:Uric Acid / Q000494:pharmacology*
chemical_list: D000091402:Prohibitins; D020543:Proteome; D012097:Repressor Proteins; D014527:Uric Acid