title: Up-regulation of prohibitin 1 is involved in the proliferation and migration of liver cancer cells.
abstract: Although prohibitin 1 (PHB) is known to be associated with tumors, there are few studies regarding the role of PHB in hepatocellular carcinoma. An earlier glycoproteomics study of ours showed that PHB is over-expressed in MHCC97-H cells, a highly metastatic liver cancer cell line, and can be captured by wheat germ agglutinin. In the present study, western blotting and reverse transcription-PCR experiments showed an approximately 2-fold up-regulation in the expression of PHB in MHCC97-H cells. However, PHB was not significantly up-regulated in MHCC97-L cells, a low-metastatic liver cancer cell line. When PHB was over-expressed in MHCC97-L cells, cell proliferation was inhibited by 35% and migration increased about 2-fold. The results of this study show that PHB is up-regulated in MHCC97-H cells and is associated with both proliferation and migration of liver cancer cells.
authors.affiliation: Institute of Biomedical Sciences, Fudan University, Shanghai 200032, China.
authors.forename: ZhiBin
authors.initials: Z
authors.lastname: Xu
authors.forename: JinHong
authors.initials: J
authors.lastname: Wu
authors.forename: XiLiang
authors.initials: X
authors.lastname: Zha
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D010449:Peptide Mapping; D000091402:Prohibitins; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
chemical_list: C000717328:PHB protein, human; D000091402:Prohibitins; D012097:Repressor Proteins