title: Quantitative proteomics of the integrin adhesome show a myosin II-dependent recruitment of LIM domain proteins.
abstract: A characteristic of integrins is their ability to transfer chemical and mechanical signals across the plasma membrane. Force generated by myosin II makes cells able to sense substrate stiffness and induce maturation of nascent adhesions into focal adhesions. In this paper, we present a comprehensive proteomic analysis of nascent and mature adhesions. The purification of integrin adhesion complexes combined with quantitative mass spectrometry enabled the identification and quantification of known and new adhesion-associated proteins. Furthermore, blocking adhesion maturation with the myosin II inhibitor blebbistatin markedly impaired the recruitment of LIM domain proteins to integrin adhesion sites. This suggests a common recruitment mechanism for a whole class of adhesion-associated proteins, involving myosin II and the zinc-finger-type LIM domain.
authors.affiliation: Department of Molecular Medicine, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, Germany.
authors.forename: Herbert B
authors.initials: HB
authors.lastname: Schiller
authors.forename: Caroline C
authors.initials: CC
authors.lastname: Friedel
authors.forename: Cyril
authors.initials: C
authors.lastname: Boulegue
authors.forename: Reinhard
authors.initials: R
authors.lastname: Fässler
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D022001:Focal Adhesions / Q000235:genetics / Q000378:metabolism*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D016023:Integrins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D051379:Mice; D024462:Myosin Type II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D016335:Zinc Fingers
chemical_list: D016326:Extracellular Matrix Proteins; D006576:Heterocyclic Compounds, 4 or More Rings; D016023:Integrins; D047908:Intracellular Signaling Peptides and Proteins; C472645:blebbistatin; D024462:Myosin Type II