title: A novel delivery system of probiotic drop and its effect on dental caries risk factors in cleft lip/palate children.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effect of the probiotic bacterium Lactobacillus reuteri on the levels of salivary mutans streptococci and lactobacilli in children with cleft lip/palate who used the novel drop containing L. reuteri.
  
  
  METHODS
  The study group consisted of 19 operated cleft lip/palate children aged 4 to 12 years. The study had a double-blind, randomized crossover design, and the experimental period consisted of four consecutive time periods. During periods 2 and 4, consisting of 25 days each, parents were instructed that their children should consume 5 drops per day (0.15 to 0.20 g) of probiotic or placebo drops produced by the same manufacturer. The probiotic drop, BioGaia Reuteri drops, contained L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289 (≥1 × 10(8) CFU/5 drops). The counts of salivary mutans streptococci and lactobacilli were evaluated using the CRT tests. The data were processed with NCSS 2007 software using chi-square and McNemar tests.
  
  
  RESULTS
  There was no statistically significant (p > .05) reduction of salivary mutans streptococci and lactobacilli after 25 days of consumption of both drops.
  
  
  CONCLUSIONS
  The novel drop containing L. reuteri may not reduce the levels of salivary mutans streptococci and lactobacilli in cleft lip/palate children.
authors.affiliation: Department of Pedodontics, Faculty of Dentistry, Yeditepe University, Istanbul, Turkey. sulecildir@yahoo.com.tr
authors.forename: Sule Kavaloglu
authors.initials: SK
authors.lastname: Cildir
authors.forename: Nuket
authors.initials: N
authors.lastname: Sandalli
authors.forename: Serpil
authors.initials: S
authors.lastname: Nazli
authors.forename: Fatos
authors.initials: F
authors.lastname: Alp
authors.forename: Esber
authors.initials: E
authors.lastname: Caglar
mesh_terms: D002648:Child; D002675:Child, Preschool; D002971:Cleft Lip / Q000382:microbiology* / Q000601:surgery; D002972:Cleft Palate / Q000382:microbiology* / Q000601:surgery; D018592:Cross-Over Studies; D003731:Dental Caries / Q000382:microbiology* / Q000517:prevention & control*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification; D052200:Limosilactobacillus reuteri; D008297:Male; D019936:Probiotics / Q000008:administration & dosage*; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects / Q000302:isolation & purification