title: Cardamonin stimulates glucose uptake through translocation of glucose transporter-4 in L6 myotubes.
abstract: Glucose transporter-4 (GLUT4) is a transmembrane protein that plays a major role in insulin-mediated glucose transport in muscle and adipocytes. For glucose transport to occur, the GLUT4 protein needs to be translocated from the intracellular pool to the plasma membrane, and certain compounds may enhance this process. The present study investigated the promotion of glucose uptake in differentiated L6 myotubes by cardamonin, isolated from Alpinia katsumadai. Cardamonin increased translocation of GLUT4 to the plasma membrane in L6 cells, but did not activate protein kinase C ζ/λ, Akt, or AMP-activated protein-kinase, all of which are known to regulate GLUT4 translocation. The glucose-uptake-promoting activity of cardamonin was not lowered by treatment with a phosphatidylinositol 3'-kinase inhibitor. These results suggest that cardamonin is a promising active compound for maintaining glucose homeostasis, and that it acts via an unknown mechanism that does not involve activation of the downstream insulin signal and AMP-activated protein kinase.
authors.affiliation: Food Science Research Center, House Wellness Foods Corporation, Itami, Japan. Yamamoto_Norio@house-wf.co.jp
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Kyuichi
authors.initials: K
authors.lastname: Kawabata
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sawada
authors.forename: Manabu
authors.initials: M
authors.lastname: Ueda
authors.forename: Itsuko
authors.initials: I
authors.lastname: Fukuda
authors.forename: Kengo
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D032429:Alpinia / Q000737:chemistry; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D047188:Chalcones / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D006706:Homeostasis / Q000187:drug effects; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D047188:Chalcones; D051275:Glucose Transporter Type 4; C436747:cardamonin; D005947:Glucose