title: Neem leaf glycoprotein partially rectifies suppressed dendritic cell functions and associated T cell efficacy in patients with stage IIIB cervical cancer.
abstract: Myeloid-derived dendritic cells (DCs) generated from monocytes obtained from stage IIIB cervical cancer (CaCx IIIB) patients show dysfunctional maturation; thus, antitumor T cell functions are dysregulated. In an objective to optimize these dysregulated immune functions, the present study is focused on the ability of neem leaf glycoprotein (NLGP), a nontoxic preparation of the neem leaf, to induce optimum maturation of dendritic cells from CaCx IIIB patients. In vitro NLGP treatment of immature DCs (iDCs) obtained from CaCx IIIB patients results in upregulated expression of various cell surface markers (CD40, CD83, CD80, CD86, and HLA-ABC), which indicates DC maturation. Consequently, NLGP-matured DCs displayed balanced cytokine secretions, with type 1 bias and noteworthy functional properties. These DCs displayed substantial T cell allostimulatory capacity and promoted the generation of cytotoxic T lymphocytes (CTLs). Although NLGP-matured DCs derived from CaCx monocytes are generally subdued compared to those with a healthy monocyte origin, considerable revival of the suppressed DC-based immune functions is noted in vitro at a fairly advanced stage of CaCx, and thus, further exploration of ex vivo and in vivo DC-based vaccines is proposed. Moreover, the DC maturating efficacy of NLGP might be much more effective in the earlier stages of CaCx, where the extent of immune dysregulation is less and, thus, the scope of further investigation may be explored.
authors.affiliation: Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute (CNCI), 37 S. P. Mookherjee Road, Kolkata 700026, India.
authors.forename: Soumyabrata
authors.initials: S
authors.lastname: Roy
authors.forename: Shyamal
authors.initials: S
authors.lastname: Goswami
authors.forename: Anamika
authors.initials: A
authors.lastname: Bose
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Chakraborty
authors.forename: Smarajit
authors.initials: S
authors.lastname: Pal
authors.forename: Atanu
authors.initials: A
authors.lastname: Haldar
authors.forename: Parthasarathi
authors.initials: P
authors.lastname: Basu
authors.forename: Jaydip
authors.initials: J
authors.lastname: Biswas
authors.forename: Rathindranath
authors.initials: R
authors.lastname: Baral
mesh_terms: D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000096:biosynthesis; D031601:Azadirachta / Q000737:chemistry*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology* / Q000302:isolation & purification; D008875:Middle Aged; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000276:immunology* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D006023:Glycoproteins; D007155:Immunologic Factors; D010940:Plant Proteins