title: [A study on effects of cisplatin and its mechanisms on human lung adenocarcinoma SLC-89 cells].
abstract: BACKGROUND
  To investigate the effects of cisplatin on proliferation, telomerase activity, cell cycle, p53, bcl-2 and proliferating cell nuclear antigen (PCNA) expressions of human lung adenocarcinoma SLC-89 cells induced by cisplatin and to find out the possible mechanisms.
  
  
  METHODS
  SLC-89 cells were treated with cisplatin of different concentrations for 72 h. Then, the proliferation of the cells was measured by MTT method, telomerase activity was measured by telomeric repeat amplification protocol with ELISA (TRAP-ELISA), and cell cycle, p53, bcl-2 and PCNA expressions of the cells were detected by flow cytometry (FCM) respectively.
  
  
  RESULTS
  Cisplatin could obviously inhibit the proliferation of the cells, and IC₅₀ value for cisplatin treatment was 18.47 mg/L. Cisplatin could obviously down-regulate telomerase activity, decrease S phase cells, increase G₀/G₁ phase cells, decline the expressions of bcl-2 and PCNA proteins and induce the expression of p53 protein of SLC-89 cells in a concentration-dependent fashion.
  
  
  CONCLUSIONS
  Cisplatin can obviously inhibit the proliferation of SLC-89, change the distribution of cell cycle, decline telomerase activity and expressions of bcl-2 and PCNA proteins, and induce expression of p53 protein, which may be the important mechanisms of cisplatin's anticancer action.
authors.affiliation: Department of Medical Experiment, Kunming General Hospital of Chengdu Military Region, Kunming, Yunnan 650032, P.R.China.
authors.forename: Rongqing
authors.initials: R
authors.lastname: Pang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Xinghua
authors.initials: X
authors.lastname: Pan
authors.forename: Buzhen
authors.initials: B
authors.lastname: Zhang
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Wu
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Chen