title: [The enhancement effect of CD59 on CD55-mediated T cell signal transduction].
abstract: OBJECTIVE
  To study the enhancement effect of glycosylphosphatidyl inositol (GPI)-anchored protein CD59 on CD55-mediated T cell signal transduction.
  
  
  METHODS
  Human Jurkat cells were divided into 3 groups: Jurkat cells(Igroup), Jurkat cells transfected with blank plasmid(II group)and Jurkat cells transfected with siRNA plasmid (III group). CD59 mRNA level was detected by RT-PCR. The cell proliferation activity of the three groups was measured by MTTcolorimetry after crosslinking anti-CD55 mAb and anti-CD59 mAb. The phosphorylation levels of Src family of protein tyrosine kinase (PTK) were investigated by immunoblot analysis, and the kinetic changes of [Ca(2+);] in T cell endochylema were determined by Laser scanning confocal microscope imaging.
  
  
  RESULTS
  CD59 expression was successfullyinhibited in IIIgroup cells after transfecting. The cell proliferation, phosphorylation levels of Src family of PTK and degree of [Ca(2+);] inIand IIgroups were increased compared with III group(P<0.05), after crosslinking of CD55 and CD59 antibodies, but there was no difference between I group and II group.
  
  
  CONCLUSIONS
  CD59 can enhance the effect of CD55-mediated T cell signal transduction.
authors.affiliation: Department of Immunology, Medical College of Qingdao University, Qingdao 266071, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Gao
authors.forename: Bei
authors.initials: B
authors.lastname: Zhan
mesh_terms: D018958:CD55 Antigens / Q000378:metabolism*; D018957:CD59 Antigens / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D020868:Gene Silencing / Q000276:immunology; D006801:Humans; D019169:Jurkat Cells; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens; D034741:RNA, Small Interfering; D019061:src-Family Kinases; D002118:Calcium