title: Therapeutic potential of propagated hepatocyte transplantation in liver failure.
abstract: BACKGROUND
  This study aimed to evaluate the therapeutic potential of intrasplenic transplantation of culture-propagated homologous hepatocytes in rats suffering from acute liver failure (ALF).
  
  
  METHODS
  ALF was induced in dipeptidyl peptidase IV-negative (DPPIV(-)) Fischer 344 rats by totally removing the two anterior liver lobes (68% of the liver) and ligating the pedicle of the right lobe (24% of the liver). Hepatocytes isolated from DPPIV(+) Fischer 344 rats were cultured for 11 d to propagate 3-fold, and the resulting hepatocytes were dubbed "culture-propagated hepatocytes (CPHEPs)". A total of 1.5 × 10(7) cells of CPHEPs were transplanted intrasplenically before ALF induction (CPHEP group). Similarly, freshly isolated hepatocytes (FIHEPs) were transplanted as a positive control (FIHEP group), and culture medium (CM) was injected into rats as a negative control (CM group).
  
  
  RESULTS
  The survival of the CPHEP group was comparable to that of the FIHEP group and longer than that of the CM group (P < 0.01). Both CPHEP and FIHEP transplantation improved blood parameters such as ammonia, total bilirubin, glutamic pyruvic transaminase, and glutamic oxaloacetic transaminase; transplantation also affected liver tissue parameters such as apoptosis rate and bromodeoxyuridine-labeling index.
  
  
  CONCLUSIONS
  Transplantation of culture-propagated homologous hepatocytes has a remarkable therapeutic potential for ALF in rats.
authors.affiliation: Department of Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Amano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hino
authors.forename: Chise
authors.initials: C
authors.lastname: Tateno
authors.forename: Kentaro
authors.initials: K
authors.lastname: Emoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Imaoka
authors.forename: Chihiro
authors.initials: C
authors.lastname: Yamasaki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Itamoto
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Tashiro
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Asahara
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohdan
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yoshizato
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D018819:Dipeptidyl Peptidase 4 / Q000009:adverse effects; D022781:Hepatocytes / Q000166:cytology / Q000637:transplantation*; D008099:Liver / Q000503:physiopathology; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000401:mortality / Q000628:therapy*; D023421:Models, Animal; D051381:Rats; D011916:Rats, Inbred F344; D016896:Treatment Outcome
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D018819:Dipeptidyl Peptidase 4