title: Kaposi's sarcoma-associated herpesvirus bacterial artificial chromosome contains a duplication of a long unique-region fragment within the terminal repeat region.
abstract: Use of the Kaposi's sarcoma-associated herpesvirus (KSHV) bacterial artificial chromosome 36 (KSHV-BAC36) genome permits reverse genetics approaches to study KSHV biology. While sequencing the complete KSHV-BAC36 genome, we noted a duplication of a 9-kb fragment of the long unique region in the terminal repeat region. This duplication covers a part of open reading frame (ORF) 19, the complete ORFs 18, 17, 16, K7, K6, and K5, and the putative ORF in the left origin of lytic replication, and it contains the BAC cassette. This observation needs to be kept in mind if viral genes located within the duplicated region are to be mutated in KSHV-BAC36.
authors.affiliation: Institute of Virology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Yakushko
authors.forename: Christian
authors.initials: C
authors.lastname: Hackmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Günther
authors.forename: Jessica
authors.initials: J
authors.lastname: Rückert
authors.forename: Marc
authors.initials: M
authors.lastname: Henke
authors.forename: Lars
authors.initials: L
authors.lastname: Koste
authors.forename: Khaled
authors.initials: K
authors.lastname: Alkharsah
authors.forename: Jens
authors.initials: J
authors.lastname: Bohne
authors.forename: Adam
authors.initials: A
authors.lastname: Grundhoff
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Schulz
authors.forename: Cornelia
authors.initials: C
authors.lastname: Henke-Gendo
mesh_terms: D022202:Chromosomes, Artificial, Bacterial; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D056916:Segmental Duplications, Genomic; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences
chemical_list: D004279:DNA, Viral