title: Assessing antibody pharmacokinetics in mice with in vivo imaging.
abstract: Recent advances in small-animal molecular imaging instrumentation combined with well characterized antibody-labeling chemistry have enabled detailed in vivo measurements of antibody distribution in mouse models. This article reviews the strengths and limitations of in vivo antibody imaging methods with a focus on positron emission tomography and single-photon emission computed tomography and a brief discussion of the role of optical imaging in this application. A description of the basic principles behind the imaging techniques is provided along with a discussion of radiolabeling methods relevant to antibodies. Practical considerations of study design and execution are presented through a discussion of sensitivity and resolution tradeoffs for these techniques as defined by modality, signaling probe (isotope or fluorophore) selection, labeling method, and radiation dosimetry. Images and analysis results from a case study are presented with a discussion of output data content and relevant informatics gained with this approach to studying antibody pharmacokinetics.
authors.affiliation: inviCRO, LLC, 2 Oliver St. Suite 611, Boston, MA 02109, USA. hoppin@invicro.com
authors.forename: Jack
authors.initials: J
authors.lastname: Hoppin
authors.forename: Kelly Davis
authors.initials: KD
authors.lastname: Orcutt
authors.forename: Jacob Y
authors.initials: JY
authors.lastname: Hesterman
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Silva
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Cheng
authors.forename: Christian
authors.initials: C
authors.lastname: Lackas
authors.forename: Mary
authors.initials: M
authors.lastname: Rusckowski
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted; D049449:Luminescence; D051379:Mice; D010599:Pharmacokinetics; D010825:Physics; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000737:chemistry; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
chemical_list: D000906:Antibodies; D019275:Radiopharmaceuticals