title: Mapping of protein- and chromatin-interactions at the nuclear lamina.
abstract: The nuclear envelope and the lamina define the nuclear periphery and are implicated in many nuclear processes including chromatin organization, transcription and DNA replication. Mutations in lamin A proteins, major components of the lamina, interfere with these functions and cause a set of phenotypically diverse diseases referred to as laminopathies. The phenotypic diversity of laminopathies is thought to be the result of alterations in specific protein- and chromatin interactions due to lamin A mutations. Systematic identification of lamin A-protein and -chromatin interactions will be critical to uncover the molecular etiology of laminopathies. Here we summarize and critically discuss recent technology to analyze lamina-protein and-chromatin interactions.
authors.affiliation: Center for Heart Failure Research, Maastricht, The Netherlands.
authors.forename: Nard
authors.initials: N
authors.lastname: Kubben
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: Voncken
authors.forename: Tom
authors.initials: T
authors.lastname: Misteli
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D006801:Humans; D034904:Lamin Type A / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000737:chemistry / Q000378:metabolism; D034881:Nuclear Lamina / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000378:metabolism
chemical_list: D002843:Chromatin; D034904:Lamin Type A; D009687:Nuclear Proteins; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D008836:Micrococcal Nuclease