title: Synthesis and characterization of Au102(p-MBA)44 nanoparticles.
abstract: The synthesis of Au(102)(p-MBA)(44) nanoparticles on a preparative scale in high yield is described. Various analytical methods are shown to give results consistent with the composition and known structure of the particles, showing the preparation is essentially homogeneous, and attesting to the validity of the methods as well. Derivatization of the particles with proteins and DNA is demonstrated, and conditions are described for imaging individual particles by cryo-EM at low electron dose, close to focus, conditions optimal for recording high-resolution details.
authors.affiliation: Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
authors.forename: Yael
authors.initials: Y
authors.lastname: Levi-Kalisman
authors.forename: Pablo D
authors.initials: PD
authors.lastname: Jadzinsky
authors.forename: Nir
authors.initials: N
authors.lastname: Kalisman
authors.forename: Hironori
authors.initials: H
authors.lastname: Tsunoyama
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tsukuda
authors.forename: David A
authors.initials: DA
authors.lastname: Bushnell
authors.forename: Roger D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D020285:Cryoelectron Microscopy; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D013058:Mass Spectrometry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D011506:Proteins / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013818:Thermogravimetry
chemical_list: D001672:Biocompatible Materials; D011506:Proteins; D012459:Salicylates; D013438:Sulfhydryl Compounds; D006046:Gold; D004247:DNA; C441201:thiosalicylic acid