title: Sox17-dependent gene expression and early heart and gut development in Sox17-deficient mouse embryos.
abstract: Sox17 is a transcription factor that is required for maintenance of the definitive endoderm in mouse embryos. By expression profiling of wild-type and mutant embryos and Sox17-overexpressing hepatoma cells, we identified genes with Sox17-dependent expression. Among the genes that were up-regulated in Sox17-null embryos and down-regulated by Sox17 expressing HepG2 cells is a set of genes that are expressed in the developing liver, suggesting that one function of Sox17 is the repression of liver gene expression, which is compatible with a role for Sox17 in maintaining the definitive endoderm in a progenitor state. Consistent with these findings, Sox17(-/-) cells display a diminished capacity to contribute to the definitive endoderm when transplanted into wild-type hosts. Analysis of gene ontology further revealed that many genes related to heart development were downregulated in Sox17-null embryos. This is associated with the defective development of the heart in the mutant embryos, which is accompanied by localised loss of Myocd-expressing cardiogenic progenitors and the malformation of the anterior intestinal portal.
authors.affiliation: Embryology Unit, Children's Medical Research Institute, New South Wales, Australia.
authors.forename: Sabine
authors.initials: S
authors.lastname: Pfister
authors.forename: Vanessa J
authors.initials: VJ
authors.lastname: Jones
authors.forename: Melinda
authors.initials: M
authors.lastname: Power
authors.forename: Germaine L
authors.initials: GL
authors.lastname: Truisi
authors.forename: Poh-Lynn
authors.initials: PL
authors.lastname: Khoo
authors.forename: Kirsten A
authors.initials: KA
authors.lastname: Steiner
authors.forename: Masami
authors.initials: M
authors.lastname: Kanai-Azuma
authors.forename: Yoshiakira
authors.initials: Y
authors.lastname: Kanai
authors.forename: Patrick P L
authors.initials: PP
authors.lastname: Tam
authors.forename: David A F
authors.initials: DA
authors.lastname: Loebel
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D004707:Endoderm / Q000196:embryology / Q000378:metabolism; D005260:Female; D041981:Gastrointestinal Tract / Q000196:embryology / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D024002:HMGB Proteins / Q000172:deficiency / Q000235:genetics*; D006321:Heart / Q000196:embryology; D056945:Hep G2 Cells; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055760:SOXF Transcription Factors / Q000172:deficiency / Q000235:genetics*; D019170:Somites / Q000196:embryology / Q000378:metabolism
chemical_list: D024002:HMGB Proteins; C109188:SOX17 protein, human; D055760:SOXF Transcription Factors; C511606:Sox17 protein, mouse; D049452:Green Fluorescent Proteins