title: Psychrobacter sanguinis sp. nov., recovered from four clinical specimens over a 4-year period.
abstract: An analysis of 16S rRNA gene sequences from archived clinical reference specimens identified a novel species of the genus Psychrobacter, of which four strains have been independently isolated from human blood. On the basis of 16S rRNA gene sequence similarity, the closest relatives with validly published names were Psychrobacter arenosus R7(T) (98.7%), P. pulmonis CECT 5989(T) (97.7%), P. faecalis Iso-46(T) (97.6%) and P. lutiphocae IMMIB L-1110(T) (97.2%). Maximum-likelihood phylogenetic analysis of 16S rRNA gene sequences showed that the isolates belonged to the genus Psychrobacter and were members of a cluster associated with Psychrobacter sp. PRwf-1, isolated from a silk snapper fish. DNA-DNA relatedness and partial 23S rRNA gene sequences also supported the finding that the isolates belonged to a species distinct from its closest phylogenetic neighbours. The predominant cellular fatty acids were C(18:1)ω9c, C(16:0), summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH), summed feature 5 (C(18:2)ω6,9c and/or anteiso-C(18:0)) and C(18:0). Biochemical and morphological analysis further supported the assignment of the four isolates to a novel species. The name Psychrobacter sanguinis sp. nov. is proposed. The type strain is 13983(T) (=DSM 23635(T)=CCUG 59771(T)).
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, P.O. Box 22002, Albany, NY 12201, USA.
authors.forename: Samantha E
authors.initials: SE
authors.lastname: Wirth
authors.affiliation: University of Puerto Rico at Humacao, Department of Biology, Humacao 00791, Puerto Rico.
authors.forename: Héctor L
authors.initials: HL
authors.lastname: Ayala-Del-Río
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, P.O. Box 22002, Albany, NY 12201, USA.
authors.forename: Jocelyn A
authors.initials: JA
authors.lastname: Cole
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, P.O. Box 22002, Albany, NY 12201, USA.
authors.forename: Donna J
authors.initials: DJ
authors.lastname: Kohlerschmidt
authors.affiliation: Department of Biomedical Sciences, State University of New York, Albany, NY 12201, USA.|Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, P.O. Box 22002, Albany, NY 12201, USA.
authors.forename: Kimberlee A
authors.initials: KA
authors.lastname: Musser
authors.affiliation: Universidad Metropolitana, School of Science and Technology, San Juan 00928, Puerto Rico.
authors.forename: Lycely Del C
authors.initials: LDC
authors.lastname: Sepúlveda-Torres
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, P.O. Box 22002, Albany, NY 12201, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Thompson
authors.affiliation: Department of Biomedical Sciences, State University of New York, Albany, NY 12201, USA.|Wadsworth Center, Bacteriology Laboratory, New York State Dept. of Health, P.O. Box 22002, Albany, NY 12201, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Wolfgang
mesh_terms: D016470:Bacteremia / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D045828:Moraxellaceae Infections / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D044096:Psychrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S