title: Elevated couch potato transcripts associated with adult diapause in the mosquito Culex pipiens.
abstract: The couch potato (cpo) cDNA that we cloned from the northern house mosquito, Culex pipiens, encodes the C-terminus containing a highly conserved RNA recognition motif (RRM). Protein structure prediction indicates a canonical RRM structure with a βαββαβ topological structure. Northern blots indicate a single mRNA band over 9.49 kb, and Southern blot analysis suggests that the cpo gene contains large introns. Highest expression was noted in first instar larvae and pupae. A comparison of nondiapausing (long daylength) and diapausing (short daylength) adult females showed no difference immediately at adult eclosion, but by day 7 and thereafter, expression of cpo was much higher in diapausing adults. When 2-month old diapausing females were transferred from short daylength to diapausing-terminating conditions of long daylength and high temperature, expression of cpo declined. Similarly, when a topical application of JH III was used to terminate diapause abundance of the cpo transcript declined. Consistent with observations in Drosophila melanogaster and several other species levels of cpo in C. pipiens are influenced by the diapause program, although the direction of change is not the same in all species.
authors.affiliation: Department of Entomology, Ohio State University, 318 West 12th Avenue, Columbus, OH 43210, USA.
authors.forename: Qirui
authors.initials: Q
authors.lastname: Zhang
authors.forename: David L
authors.initials: DL
authors.lastname: Denlinger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003465:Culex / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004956:Estivation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011679:Pupa / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA
chemical_list: D019476:Insect Proteins; D009687:Nuclear Proteins