title: Immobilization of β-galactosidase on novel polymers having Schiff bases.
abstract: We have developed a strategy to immobilize β-galactosidase as a model enzyme by using polymeric supports having Schiff bases, which were prepared from (aminomethyl)polystyrene and 2-phenlyindole-3-carboxaldehyde by condensation. β-galactosidase was immobilized onto the new polymer supports via covalent bonds. The influence of temperature, pH, reusability, and storage capacity on the free and immobilized β-galactosidase was investigated. Our results indicate that the (aminomethyl)polystyrene with Schiff bases is most suitable for the immobilization of β-galactosidase. These kinds of new supports can be used for the immobilization of β-galactosidase due to their strong storage capacity and reusability.
authors.affiliation: Department of Chemistry, Faculty of Science and Arts, Bozok University, Yozgat, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Aynacı
authors.forename: Nurşen
authors.initials: N
authors.lastname: Sarı
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Tümtürk
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D012545:Schiff Bases / Q000737:chemistry*; D013696:Temperature; D001616:beta-Galactosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000447:Aldehydes; D004800:Enzymes, Immobilized; D011108:Polymers; D011137:Polystyrenes; D012545:Schiff Bases; D001616:beta-Galactosidase