title: Colocalisation of plasma derived apo B lipoproteins with cerebral proteoglycans in a transgenic-amyloid model of Alzheimer's disease.
abstract: Alzheimer's disease (AD) is characterized by cerebral proteinaceous deposits comprised of amyloid beta (Aβ). Evidence suggests that enhanced blood-to-brain delivery of Aβ occurs when plasma concentration is increased, exacerbating amyloidosis. In blood, significant Aβ is associated with apolipoprotein (apo) B lipoproteins. In this study, immunofluorescent microscopy was utilised to explore if there is an association between apo B lipoproteins and proteoglycan expression within Aβ-rich plaques in transgenic-amyloid mice. Focal accumulation of apo B was found with Aβ-plaque in APP/PS1 mice. There was enrichment in the proteoglycans, agrin, perlecan, biglycan and decorin within the core of dense Aβ-plaque. Perlecan, biglycan and decorin were positively associated with apo B lipoprotein abundance within amyloid plaque consistent with a cause-for-retention effect. These findings show that proteoglycans are an integral component of Aβ deposits in APP/PS1 mice. This study suggests that some proteoglycans contribute to Aβ retention, whilst other proteoglycans have different functions in the aetiology of AD.
authors.affiliation: Curtin Health Innovation Research Institute, and The Australian Technology Network Centre for Metabolic Fitness, Curtin University, Bentley Campus, Kent Street, Perth 6102, Australia.
authors.forename: Virginie
authors.initials: V
authors.lastname: Lam
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Takechi
authors.forename: Menuka Madhavi Sompala
authors.initials: MM
authors.lastname: Pallebage-Gamarallage
authors.forename: Susan
authors.initials: S
authors.lastname: Galloway
authors.forename: John C L
authors.initials: JC
authors.lastname: Mamo
mesh_terms: D018171:Agrin / Q000378:metabolism; D000544:Alzheimer Disease / Q000097:blood* / Q000235:genetics; D016229:Amyloid beta-Peptides / Q000097:blood* / Q000235:genetics; D000818:Animals; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000097:blood*; D058578:Biglycan / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D058575:Decorin / Q000097:blood; D004195:Disease Models, Animal; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058225:Plaque, Amyloid / Q000097:blood* / Q000473:pathology; D011509:Proteoglycans / Q000097:blood*
chemical_list: D018171:Agrin; D016229:Amyloid beta-Peptides; C415826:Apob protein, mouse; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D058578:Biglycan; C546879:Dcn protein, mouse; D058575:Decorin; D019812:Heparan Sulfate Proteoglycans; D011509:Proteoglycans; C071927:perlecan