title: Fluorescent substrates for polyamine catabolic enzymes and transport.
abstract: The most widely used methods for measuring polyamine enzyme activities are radioisotope methods that measure the radioactivity of compounds produced from radiolabeled substrate by the enzyme reaction. Several fluorescent polyamines have been developed for the measurement of the polyamine transport system (PTS) or transglutaminase. Although fluorophores in the fluorescent polyamines may affect the affinity of the polyamine moiety to the enzyme protein, the assays that use fluorescent substrate are sensitive and simple for common laboratory usage.In this chapter, the uses of dansyl polyamines with a simple high-performance liquid chromatography system for the measurement of the PTS and polyamine catabolic enzymes including spermidine/spermine N¹-acetyltransferase and N¹-acetylpolyamine oxidase are described.
authors.affiliation: Laboratory of Cellular Physiology, Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Takao
authors.forename: Akira
authors.initials: A
authors.lastname: Shirahata
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000138:chemical synthesis; D057075:Enzyme Assays; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000201:enzymology; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D051381:Rats; D013095:Spermidine / Q000138:chemical synthesis / Q000378:metabolism; D013096:Spermine / Q000138:chemical synthesis / Q000378:metabolism; D013379:Substrate Specificity; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D013095:Spermidine; D013096:Spermine; D000099228:Diamine N-Acetyltransferase