title: Investigation of the significance of Oil Red O-positive macrophage excess in bronchoalveolar lavage fluid during HIV infection.
abstract: OBJECTIVE
  To assess the significance of increased levels of Oil Red O-positive macrophages (ORO-PM) in bronchoalveolar lavage fluids (BALFs) from HIV-positive patients.
  
  
  METHODS
  Cytological data for seventy BALF samples from 66 consecutive HIV-infected patients were analysed according to antiretroviral therapy regimen, presence of Pneumocystis jiroveci infection, blood CD4(+) T cell count, HIV-1 viral load and plasma lipid levels. Non-parametric tests were used to compare the values between groups.
  
  
  RESULTS
  The percentages of ORO-PM were high in this group: 40% [6-80] (median [interquartile range]). They were positively correlated with the BALF total cell count, 21% [5-48.5] for <300 cells/mm(3) and 60% [26.5-80] for >300 cells/mm(3) (P<0.01) but inversely correlated with the percentage of BALF lymphocytes, 50% [20-80] for <15% lymphocytes and 11.5% [2-47] for ≥15% lymphocytes (P<0.01). Antiretroviral therapy with or without protease inhibitors, plasma lipid levels, HIV-1 viral load, blood CD4(+) T cell count or presence of a Pneumocystis jiroveci infection were not correlated with the ORO-PM status.
  
  
  CONCLUSIONS
  Significantly increased numbers of ORO-PM were correlated with high total cell counts and low lymphocyte counts in BALF, irrespective of disease activity or treatment. Extended work on a larger series of patients needs to be conducted.
authors.affiliation: Department of Pathology and Histology-Cytology, Toulouse Rangueil Hospital, Toulouse Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Lacoste-Collin
authors.forename: G
authors.initials: G
authors.lastname: Martin-Blondel
authors.forename: C
authors.initials: C
authors.lastname: Basset-Léobon
authors.forename: V
authors.initials: V
authors.lastname: Lauwers-Cancès
authors.forename: D
authors.initials: D
authors.lastname: d'Aure
authors.forename: J
authors.initials: J
authors.lastname: Aziza
authors.forename: A
authors.initials: A
authors.lastname: Berry
authors.forename: B
authors.initials: B
authors.lastname: Marchou
authors.forename: M B
authors.initials: MB
authors.lastname: Delisle
authors.forename: M
authors.initials: M
authors.lastname: Courtade-Saïdi
mesh_terms: D000328:Adult; D000368:Aged; D001391:Azo Compounds / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002452:Cell Count; D005260:Female; D015658:HIV Infections / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D008264:Macrophages / Q000382:microbiology / Q000473:pathology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D045363:Pneumocystis carinii; D013194:Staining and Labeling; D055815:Young Adult
chemical_list: D001391:Azo Compounds; C011049:oil red O