title: The influence of hyperbaric oxygen treatment on the healing of experimental defects filled with different bone graft substitutes.
abstract: To assess potential effects of hyperbaric oxygen (HBOT) on artificial bone grafts, β - Tricalcium phosphate (β-TCP) and calcium phosphate coated bovine bone (CPCBB) substitutes were applied to standard bone defects in rat tibiae. The control defects were left empty. Half of the animals received 60 minutes of 2.4 atmosphere absolute (ATA) of HBOT. Rats were sacrificed at one, two and four weeks. Bone healing was assessed histologically and histomorphometrically using light microscopy. The periosteum over the bone defects was examined ultrastructurally. Cardiac blood was collected to determine the serum osteocalcin levels. The HBOT increased new bone formation in the unfilled controls and β-TCP groups and significantly decreased cartilage matrix and fibrous tissue formations in all groups. Active osteoblasts and highly organized collagen fibrils were prominent in the periosteum of β-TCP and control groups. Serum osteocalcin levels also increased with HBOT. The healing of defects filled with CPCBB was similar to the controls and it did not respond to HBOT. These findings suggested that the HBOT had beneficial effects on the healing of unfilled bone defects and those filled with β-TCP bone substitute but not with CPCBB, indicating a material-specific influence pattern of HBOT.
authors.affiliation: Istanbul University, Faculty of Dentistry, Department of Oral Surgery, Istanbul, Turkey. ysirin@istanbul.edu.tr
authors.forename: Yigit
authors.initials: Y
authors.lastname: Sirin
authors.forename: Vakur
authors.initials: V
authors.lastname: Olgac
authors.forename: Semra
authors.initials: S
authors.lastname: Dogru-Abbasoglu
authors.forename: Leyla
authors.initials: L
authors.lastname: Tapul
authors.forename: Samil
authors.initials: S
authors.lastname: Aktas
authors.forename: Sinan
authors.initials: S
authors.lastname: Soley
mesh_terms: D000818:Animals; D001846:Bone Development; D018786:Bone Substitutes; D016025:Bone Transplantation; D006931:Hyperbaric Oxygenation; D008297:Male; D015675:Osteocalcin / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018786:Bone Substitutes; D015675:Osteocalcin