title: Alteration of plasma membrane-bound redox systems of iron deficient pea roots by chitosan.
abstract: Iron is essential for all living organisms and plays a crucial role in pathogenicity. This study presents the first proteome analysis of plasma membranes isolated from pea roots. Protein profiles of four different samples (+Fe, +Fe/Chitosan, -Fe, and -Fe/Chitosan) were compared by native IEF-PAGE combined with in-gel activity stains and DIGE. Using DIGE, 89 proteins of interest were detected in plasma membrane fractions. Data revealed a differential abundance of several spots in all samples investigated. In comparison to the control and -FeCh the abundance of six protein spots increased whereas 56 spots decreased in +FeCh. Altered protein spots were analyzed by MALDI-TOF-TOF mass spectrometry. Besides stress-related proteins, transport proteins and redox enzymes were identified. Activity stains after native PAGE and spectrophotometric measurements demonstrated induction of a ferric-chelate reductase (-Fe) and a putative respiratory burst oxidase homolog (-FeCh). However, the activity of the ferric-chelate reductase decreased in -Fe plants after elicitor treatment. The activity of plasma membrane-bound class III peroxidases increased after elicitor treatment and decreased under iron-deficiency, whereas activity of quinone reductases decreased mostly after elicitor treatment. Possible functions of proteins identified and reasons for a weakened pathogen response of iron-deficient plants were discussed.
authors.affiliation: University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Plant Physiology, Ohnhorststraße 18, D-22609 Hamburg, Germany.
authors.forename: Claudia-Nicole
authors.initials: CN
authors.lastname: Meisrimler
authors.forename: Sebastien
authors.initials: S
authors.lastname: Planchon
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Sabine
authors.initials: S
authors.lastname: Lüthje
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D048271:Chitosan / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D038181:FMN Reductase / Q000096:biosynthesis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007501:Iron / Q000494:pharmacology; D000090463:Iron Deficiencies; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis; D010084:Oxidation-Reduction; D018532:Pisum sativum / Q000378:metabolism; D010544:Peroxidases / Q000096:biosynthesis; D018517:Plant Roots / Q000201:enzymology*; D025262:Vacuolar Proton-Translocating ATPases / Q000096:biosynthesis
chemical_list: D048271:Chitosan; D007501:Iron; D010544:Peroxidases; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; C023903:ferric citrate iron reductase; D025262:Vacuolar Proton-Translocating ATPases