title: A rapid determination of drug candidate tyrosol galactoside in rat plasma by HPLC and its application to the pharmacokinetics study.
abstract: A simple and sensitive HPLC method has been developed and validated for the determination of tyrosol galactoside (TG) in rat plasma. After one-step protein precipitation with methanol, plasma samples were separated on an Ultimate AQ-C18 column (150 mm×4.6 mm, 5 μm) using acetonitrile-water (7:93, v/v) as mobile phase at a flow rate of 1.2 mL/min. The ultraviolet detection wavelength was set at 275 nm. The lower limit of quantification was 1.140 μg/mL. The calibration curve was linear over a concentration range of 1.140-228.0 μg/mL. The assay accuracy and precision were within the range of 99.6-103.0 and 2.17-6.23%, respectively. The developed method was successfully applied to the pharmacokinetics study of TG in rats after intravenous and oral administration. The bioavailability of TG in rats is 27.9%.
authors.affiliation: Department of Pharmacy, The Second Affiliated Hospital, and Department of Medicinal Chemistry, Fourth Military Medical University, 17 Changlexi Street, Xi'an, 710032, People's Republic of China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yating
authors.initials: Y
authors.lastname: Deng
authors.forename: Tianrao
authors.initials: T
authors.lastname: Shi
authors.forename: Xueying
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005697:Galactosides / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D000975:Antioxidants; D005697:Galactosides; C557337:tyrosol galactoside; D010626:Phenylethyl Alcohol