title: The feasibility of non-viral gene transfer to the diaphragm in vivo.
abstract: Gene transfer using electroporation is an essential method for the study of developmental biology, especially to understand the internal control of degeneration and apoptosis of the muscle cells that occurs earlier and quicker than the usual degeneration process occurring by aging. Such experimental studies may have a role in developing new strategies for treating patients suffering from inherited primary myopathies such as Duchenne muscular dystrophy (DMD). The present study was designed to evaluate the feasibility of electroporation mediated transfer of reporter genes to the diaphragm in vivo. This is the first report of gene transfer of naked plasmid DNA into the diaphragm muscle in vivo using electroporation. Our results showed that in vivo gene transfer of naked plasmid DNA into the diaphragm muscle using electroporation is feasible.
authors.affiliation: Division of General Thoracic Surgery University Hospital Berne, Switzerland. morris.beshay@evkb.de
authors.forename: Morris
authors.initials: M
authors.lastname: Beshay
authors.forename: Amiq
authors.initials: A
authors.lastname: Gazdhar
authors.forename: Mathias
authors.initials: M
authors.lastname: Gugger
authors.forename: Marc
authors.initials: M
authors.lastname: Reymond
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Schmid
mesh_terms: D000818:Animals; D004247:DNA; D003964:Diaphragm / Q000166:cytology*; D018274:Electroporation / Q000379:methods*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D008297:Male; D020388:Muscular Dystrophy, Duchenne / Q000628:therapy; D010957:Plasmids; D051381:Rats; D011916:Rats, Inbred F344; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D004247:DNA; D001616:beta-Galactosidase