title: Favourable prognostic value of antibodies anti-HSP20 in patients with cystic echinococcosis: a differential immunoproteomic approach.
abstract: Seeking biomarkers reflecting disease development in cystic echinococcosis (CE), we used a proteomic approach linked to immunological characterisation for the identification of respective antigens. Two-dimensional gel electrophoresis (2-DE) of sheep hydatid fluid, followed by immunoblot analysis (IB) with sera from patients with distinct phases of disease, enabled us to identify by mass spectrometry heat shock protein 20 (HSP20) as a potential marker of active CE. Using IB, antibodies specific to the 34 kDa band of HSP20 were detected in sera from 61/95 (64%) patients with CE, but not in sera from healthy subjects. IB revealed anti-HSP20 antibodies in a higher percentage of sera from patients with active disease than in sera from patients with inactive disease (81 vs. 24%; P = 10(-4)). These primary results were confirmed in a long-term follow-up study after pharmacological and surgical treatment. Herewith anti-HSP20 antibody levels significantly decreased over the course of treatment in sera from patients with cured disease, relative to sera from patients with progressive disease (P = 0·017). Thus, during CE, a comprehensive strategy of proteomic identification combined with immunological validation represents a promising approach for the identification of biomarkers useful for the prognostic assessment of treatment of CE patients.
authors.affiliation: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Vacirca
authors.forename: M
authors.initials: M
authors.lastname: Perdicchio
authors.forename: E
authors.initials: E
authors.lastname: Campisi
authors.forename: F
authors.initials: F
authors.lastname: Delunardo
authors.forename: E
authors.initials: E
authors.lastname: Ortona
authors.forename: P
authors.initials: P
authors.lastname: Margutti
authors.forename: A
authors.initials: A
authors.lastname: Teggi
authors.forename: B
authors.initials: B
authors.lastname: Gottstein
authors.forename: A
authors.initials: A
authors.lastname: Siracusano
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D004443:Echinococcosis / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology* / Q000601:surgery; D004446:Echinococcus / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D050886:HSP20 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D011379:Prognosis; D020543:Proteome / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015415:Biomarkers; D050886:HSP20 Heat-Shock Proteins; D020543:Proteome