title: Solid-phase peptide synthesis using microwave irradiation.
abstract: Since the advent of solid-phase peptide synthesis (SPPS) in the late 1950s, numerous advancements in the underlying chemistry (i.e., orthogonal protection strategy, coupling reagents, and solid support matrices) have greatly improved the efficiency of the technique. More recently, application of microwave radiation to SPPS has been found to reduce reaction time and/or increase the initial purity of synthetic peptide products. In this protocol, conditions are described to accomplish rapid peptide coupling and 9-fluorenylmethoxycarbonyl (Fmoc) removal reactions under temperature-controlled conditions in either a manual or automated synthesis format using a microwave reactor. These microwave-assisted peptide synthesis procedures have been used to rapidly prepare a "difficult" peptide sequence from the acyl carrier protein, ACP(65-74), in less than 3 h and the reduced, linear precursor to human hepcidin, in high initial purity.
authors.affiliation: Peptide Research & Discovery, Amgen, Thousand Oaks, CA, USA.
authors.forename: Justin K
authors.initials: JK
authors.lastname: Murray
authors.forename: Jennifer
authors.initials: J
authors.lastname: Aral
authors.forename: Les P
authors.initials: LP
authors.lastname: Miranda
mesh_terms: D000213:Acyl Carrier Protein / Q000138:chemical synthesis / Q000737:chemistry; D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D005449:Fluorenes; D064451:Hepcidins; D006801:Humans; D008872:Microwaves; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C496789:9-fluorenylmethoxycarbonyl; D000213:Acyl Carrier Protein; D023181:Antimicrobial Cationic Peptides; D005449:Fluorenes; C578702:HAMP protein, human; D064451:Hepcidins; D010455:Peptides