title: Total abdominal 18F-FDG uptake reflects intestinal adenoma burden in Apc mutant mice.
abstract: Apc mutant (Apc(Min)) mice develop multiple adenomas in their intestines and are widely used to study colorectal carcinogenesis and chemopreventive approaches. Molecular imaging of intestinal adenomas could potentially provide noninvasive longitudinal evaluation of these lesions in living mice. Therefore, the aim of this study was to investigate the role of (18)F-FDG PET in the Apc(Min) mouse model.
  
  
  METHODS
  Apc(Min) mice (n = 8) fed a purified diet were imaged serially after injection of (18)F-FDG at age 9 and 12 wk using a small-animal PET scanner. Abdominal uptake of the tracer was quantified. After dissection, intestines were imaged separately, and intestinal tracer uptake was quantified. Tracer distribution was compared with results from microscopic examination regarding adenoma number and size. Thereafter, findings were validated serially in 20 Apc(Min) mice aged 6, 8, 10, and 12 wk that received standard chow to increase adenoma numbers. In vivo abdominal (18)F-FDG uptake was correlated with microscopy results.
  
  
  RESULTS
  Microscopic examination showed that the mice developed 25-35 intestinal adenomas at age 12 wk. Ex vivo (18)F-FDG PET of the dissected intestines visualized all large adenomas and most small adenomas. Ex vivo total intestinal (18)F-FDG uptake correlated with in vivo total abdominal uptake and with the number of large adenomas at age 9 and 12 wk. At 12 wk, there was a clear correlation between in vivo abdominal tracer uptake and the number of large adenomas but not the total number of lesions.
  
  
  CONCLUSIONS
  Intestinal adenomas in Apc(Min) mice are metabolically active lesions that take up (18)F-FDG. Abdominal (18)F-FDG uptake at age 12 wk serves as a readout modality for large intestinal adenomas.
authors.affiliation: Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands.
authors.forename: Dianne M
authors.initials: DM
authors.lastname: Heijink
authors.forename: Jan H
authors.initials: JH
authors.lastname: Kleibeuker
authors.forename: Wouter B
authors.initials: WB
authors.lastname: Nagengast
authors.forename: Dorenda
authors.initials: D
authors.lastname: Oosterhuis
authors.forename: Adrienne H
authors.initials: AH
authors.lastname: Brouwers
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Koornstra
authors.forename: Steven
authors.initials: S
authors.lastname: de Jong
authors.forename: Elisabeth G E
authors.initials: EG
authors.lastname: de Vries
mesh_terms: D000005:Abdomen / Q000000981:diagnostic imaging; D000236:Adenoma / Q000000981:diagnostic imaging* / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015179:Colorectal Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics / Q000378:metabolism*; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D017491:Genes, APC; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18