title: LacdiNAc (GalNAcβ1-4GlcNAc) contributes to self-renewal of mouse embryonic stem cells by regulating leukemia inhibitory factor/STAT3 signaling.
abstract: Self-renewal of mouse embryonic stem cells (mESCs) is maintained by leukemia inhibitory factor (LIF)/signal transducer and activator of transcription (STAT3) signaling. However, this signaling control does not function in neither mouse epiblast stem cells (mEpiSCs) nor human ESCs (hESCs) or human induced pluripotent stem cells (hiPSCs). To date, the underlying molecular mechanisms that determine this differential LIF-responsiveness have not been clarified. Here, we show that the cell surface glycan LacdiNAc (GalNAcβ1-4GlcNAc) is required for LIF/STAT3 signaling. Undifferentiated state mESCs expressed LacdiNAc at a higher level than differentiated state cells. Knockdown of β4GalNAc-T3 reduced LacdiNAc expression and caused a decrease in LIF/STAT3 signaling that lessened the rate of self-renewal of mESCs. A biochemical analysis showed that LacdiNAc expression on LIF receptor (LIFR) and gp130 was required for the stable localization of the receptors with lipid raft/caveolar components, such as caveolin-1. This localization is required for transduction of a sufficiently strong LIF/STAT3 signal. In primed state pluripotent stem cells, such as hiPSCs and mEpiSC-like cells produced from mESCs, LacdiNAc expression on LIFR and gp130 was extremely weak and the level of localization of these receptors on rafts/caveolae was also low. Furthermore, knockdown of β4GalNAc-T3 decreased LacdiNAc expression and reduced the efficiency of reversion of primed state mEpiSC-like cells into naïve state mESCs. These findings show that the different LIF-responsiveness of naïve state (mESCs) and primed state (mEpiSCs, hESCs, and hiPSCs) cells is dependent on the expression of LacdiNAc on LIFR and gp130 and that this expression is required for the induction and maintenance of the naïve state.
authors.affiliation: Laboratory of Cell Biology, Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Sasaki
authors.forename: Masahito
authors.initials: M
authors.lastname: Shinomi
authors.forename: Kazumi
authors.initials: K
authors.lastname: Hirano
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ui-Tei
authors.forename: Shoko
authors.initials: S
authors.lastname: Nishihara
mesh_terms: D000818:Animals; D051242:Caveolin 1; D002454:Cell Differentiation; D002478:Cells, Cultured; D050822:Cytokine Receptor gp130 / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D015151:Immunoblotting; D007785:Lactose / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051242:Caveolin 1; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507841:Lifr protein, mouse; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D050822:Cytokine Receptor gp130; C093701:N-acetylgalactosaminyl-1-4-N-acetylglucosamine; D017350:N-Acetylgalactosaminyltransferases; D007785:Lactose