title: General method for selective labelling of double-chain cysteine-rich peptides with a lanthanide chelate via solid-phase synthesis.
abstract: The use of lanthanides in preference to radioisotopes as probes for various biological assays has gained enormous popularity. The introduction of lanthanide chelates to peptides/proteins can be carried out either in solution using a commercially available labelling kit or by solid-phase peptide synthesis using an appropriate lanthanide chelate. Herein, a detailed protocol for the latter is provided for the labelling of peptides or small proteins with diethylenetriamine-N, N, N″, N″-tetra-tert-butyl acetate-N'-acetic acid (DTPA) chelate or other similar chelates on a solid support using a chimeric insulin-like peptide composed of human insulin-like peptide 5 (INSL5) A-chain and relaxin-3 B-chain as a model peptide. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.
authors.affiliation: Howard Florey Institute, University of Melbourne, Victoria 3010, Australia.
authors.forename: Fazel
authors.initials: F
authors.lastname: Shabanpoor
authors.forename: Frances
authors.initials: F
authors.lastname: Separovic
authors.forename: John D
authors.initials: JD
authors.lastname: Wade
mesh_terms: D003545:Cysteine / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000138:chemical synthesis / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D013058:Mass Spectrometry; D008956:Models, Chemical; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D007328:Insulin; D028581:Lanthanoid Series Elements; C084797:Leydig insulin-like protein; D010455:Peptides; D011506:Proteins; D003545:Cysteine