title: Characterization of ocular tissues using microindentation and hertzian viscoelastic models.
abstract: OBJECTIVE
  The authors applied a novel microindentation technique to characterize biomechanical properties of small ocular and orbital tissue specimens using the hertzian viscoelastic formulation, which defines material viscoelasticity in terms of the contact pressure required to maintain deformation by a harder body.
  
  
  METHODS
  They used a hard spherical indenter having 100 nm displacement and 100 μg force precision to impose small deformations on fresh bovine sclera, iris, crystalline lens, kidney fat, orbital pulley tissue, and orbital fatty tissue; normal human orbital fat, eyelid fat, and dermal fat; and orbital fat associated with thyroid eye disease. For each tissue, stress relaxation testing was performed using a range of ramp displacements. Results for single displacements were used to build quantitative hertzian models that were, in turn, compared with behavior for other displacements. Findings in orbital tissues were correlated with quantitative histology.
  
  
  RESULTS
  Viscoelastic properties of small specimens of orbital and ocular tissues were reliably characterized over a wide range of rates and displacements by microindentation using the hertzian formulation. Bovine and human orbital fatty tissues exhibited highly similar elastic and viscous behaviors, but all other orbital tissues exhibited a wide range of biomechanical properties. Stiffness of fatty tissues tissue depended strongly on the connective tissue content.
  
  
  CONCLUSIONS
  Relaxation testing by microindentation is a powerful method for characterization of time-dependent behaviors of a wide range of ocular and orbital tissues using small specimens, and provides data suitable to define finite element models of a wide range of tissue interactions.
authors.affiliation: Department of Ophthalmology, University of California, Los Angeles, California, USA.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Yoo
authors.forename: Jason
authors.initials: J
authors.lastname: Reed
authors.forename: Andrew
authors.initials: A
authors.lastname: Shin
authors.forename: Jennifer
authors.initials: J
authors.lastname: Kung
authors.forename: James K
authors.initials: JK
authors.lastname: Gimzewski
authors.forename: Vadims
authors.initials: V
authors.lastname: Poukens
authors.forename: Robert A
authors.initials: RA
authors.lastname: Goldberg
authors.forename: Ronald
authors.initials: R
authors.lastname: Mancini
authors.forename: Mehryar
authors.initials: M
authors.lastname: Taban
authors.forename: Ronald
authors.initials: R
authors.lastname: Moy
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Demer
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D002417:Cattle; D003238:Connective Tissue / Q000378:metabolism; D004547:Elastic Tissue / Q000502:physiology*; D004548:Elasticity / Q000502:physiology*; D005123:Eye / Q000378:metabolism*; D020342:Finite Element Analysis; D049970:Graves Ophthalmopathy / Q000378:metabolism; D006801:Humans; D007498:Iris / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism; D008954:Models, Biological; D009915:Orbit / Q000378:metabolism; D009916:Orbital Diseases / Q000378:metabolism*; D012590:Sclera / Q000378:metabolism