title: Footrot on a sheep breeding farm in the Himalayan state of Jammu and Kashmir.
abstract: In the present study ovine footrot was detected clinically on a sheep farm in the Himalayan state of Jammu and Kashmir. Dichelobacter nodosus was confirmed by culture and polymerase chain reaction (PCR) using species-specific 16S ribosomal RNA primers. When cultured, the organism appeared as flat colourless colonies having a fine granulated structure with irregular margins, and showing characteristic Gram-negative rods with swollen ends. Detection by PCR from cultured bacteria resulted in amplification of a 783 base pairs (bp) product. Serogrouping by multiplex PCR using group (A-I)-specific primers revealed the presence of serogroup B-specific bands of 283 bp.
authors.affiliation: Division of Veterinary Microbiology & Immunology, Faculty of Veterinary Sciences & Animal Husbandry, S.K. University of Agricultural Sciences & Technology of Jammu, RS Pura-181 102, Jammu, Jammu and Kashmir, India. aniltaku@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Taku
authors.forename: R
authors.initials: R
authors.lastname: Chhabra
authors.forename: B A
authors.initials: BA
authors.lastname: War
mesh_terms: D000818:Animals; D020376:Dichelobacter nodosus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D004196:Disease Outbreaks / Q000662:veterinary*; D005535:Foot Rot / Q000453:epidemiology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006724:Hoof and Claw / Q000382:microbiology / Q000473:pathology; D007194:India / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012313:RNA; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology; D013045:Species Specificity
chemical_list: C034477:RNA primers; D012336:RNA, Ribosomal, 16S; D012313:RNA