title: Downregulation of protein kinase Cα was involved in selenite-induced apoptosis of NB4 cells.
abstract: We revealed in our previous research that sodium selenite induced obvious apoptosis of human leukemia NB4 cells, with reactive oxygen species (ROS), mitochondrial apoptosis pathway, and endoplasmic reticulum stress (ER stress) involved. In the present study, we revealed protein kinase Ca (PKCalpha) was dramatically downregulated in selenite-induced apoptosis, which was mediated by ROS. Besides, we confirmed PKCalpha played an antiapoptotic role through its effects on ERK1/2 and Akt, while its downregulation was attributed to caspase-3 and PP2Ac under the regulation of ROS. In summary, we speculated that in apoptosis of NB4 cells induced by selenite, PKCalpha functioned to counteract apoptosis, thus its downregulation seemed a mechanism aggravating apoptosis.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Beijing, China.
authors.forename: Zhu-shi
authors.initials: ZS
authors.lastname: Li
authors.forename: Ke-jian
authors.initials: KJ
authors.lastname: Shi
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Guan
authors.forename: Qian
authors.initials: Q
authors.lastname: Jiang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Cai-min
authors.initials: CM
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000502:physiology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy / Q000473:pathology; D051571:Protein Kinase C-alpha / Q000037:antagonists & inhibitors*; D054648:Protein Phosphatase 2 / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D017382:Reactive Oxygen Species; D018038:Sodium Selenite / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D051057:Proto-Oncogene Proteins c-akt; D051571:Protein Kinase C-alpha; D048049:Extracellular Signal-Regulated MAP Kinases; D054648:Protein Phosphatase 2; D053148:Caspase 3; D018038:Sodium Selenite