title: A cysteine endopeptidase ("dionain") is involved in the digestive fluid of Dionaea muscipula (Venus's fly-trap).
abstract: The carnivorous plant Dionaea muscipula (Venus's flytrap) secretes proteinases into the digestive fluid to digest prey proteins. In this study, we obtained evidence that the digestive fluid contains a cysteine endopeptidase, presumably belonging to the papain family, through inhibitor studies and partial amino acid sequencing of the major SDS-PAGE band protein. The name "dionain" is proposed for the enzyme.
authors.affiliation: School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan. kenjitak@toyaku.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Takahashi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Wataru
authors.initials: W
authors.lastname: Nishii
authors.forename: Keiko
authors.initials: K
authors.lastname: Kubota
authors.forename: Chiaki
authors.initials: C
authors.lastname: Shibata
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Isobe
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
mesh_terms: D000595:Amino Acid Sequence; D003374:Coumarins / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004063:Digestion; D004151:Dipeptides / Q000494:pharmacology; D031257:Droseraceae / Q000201:enzymology* / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data
chemical_list: D003374:Coumarins; D015853:Cysteine Proteinase Inhibitors; D004151:Dipeptides; C021159:benzyloxycarbonyl-phenylalanylarginine-4-methylcoumaryl-7-amide; D003546:Cysteine Endopeptidases