title: Impact of salmonella infection on host hormone metabolism revealed by metabolomics.
abstract: The interplay between pathogens and their hosts has been studied for decades using targeted approaches, such as the analysis of mutants and host immunological responses. Although much has been learned from such studies, they focus on individual pathways and fail to reveal the global effects of infection on the host. To alleviate this issue, high-throughput methods, such as transcriptomics and proteomics, have been used to study host-pathogen interactions. Recently, metabolomics was established as a new method to study changes in the biochemical composition of host tissues. We report a metabolomic study of Salmonella enterica serovar Typhimurium infection. Our results revealed that dozens of host metabolic pathways are affected by Salmonella in a murine infection model. In particular, multiple host hormone pathways are disrupted. Our results identify unappreciated effects of infection on host metabolism and shed light on mechanisms used by Salmonella to cause disease and by the host to counter infection.
authors.affiliation: Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: L Caetano M
authors.initials: LC
authors.lastname: Antunes
authors.forename: Ellen T
authors.initials: ET
authors.lastname: Arena
authors.forename: Alfredo
authors.initials: A
authors.lastname: Menendez
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Rosana B R
authors.initials: RB
authors.lastname: Ferreira
authors.forename: Michelle M C
authors.initials: MM
authors.lastname: Buckner
authors.forename: Petra
authors.initials: P
authors.lastname: Lolic
authors.forename: Lufiani L
authors.initials: LL
authors.lastname: Madilao
authors.forename: Jörg
authors.initials: J
authors.lastname: Bohlmann
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
authors.forename: B Brett
authors.initials: BB
authors.lastname: Finlay
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005583:Fourier Analysis; D006790:Host-Parasite Interactions / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000378:metabolism*