title: Ex vivo culture of patient tissue & examination of gene delivery.
abstract: This video describes the use of patient tissue as an ex vivo model for the study of gene delivery. Fresh patient tissue obtained at the time of surgery is sliced and maintained in culture. The ex vivo model system allows for the physical delivery of genes into intact patient tissue and gene expression is analysed by bioluminescence imaging using the IVIS detection system. The bioluminescent detection system demonstrates rapid and accurate quantification of gene expression within individual slices without the need for tissue sacrifice. This slice tissue culture system may be used in a variety of tissue types including normal and malignant tissue and allows us to study the effects of the heterogeneous nature of intact tissue and the high degree of variability between individual patients. This model system could be used in certain situations as an alternative to animal models and as a complementary preclinical mode prior to entering clinical trial.
authors.affiliation: Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork.
authors.forename: Simon
authors.initials: S
authors.lastname: Rajendran
authors.forename: Slawomir
authors.initials: S
authors.lastname: Salwa
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Gao
authors.forename: Sabin
authors.initials: S
authors.lastname: Tabirca
authors.forename: Deirdre
authors.initials: D
authors.lastname: O'Hanlon
authors.forename: Gerald C
authors.initials: GC
authors.lastname: O'Sullivan
authors.forename: Mark
authors.initials: M
authors.lastname: Tangney
mesh_terms: D018014:Gene Transfer Techniques; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D046509:Tissue Culture Techniques / Q000379:methods*