title: Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China.
abstract: Four gram-negative, aerobic, motile, non-spore, forming rods with a wide pH and temperature range for growth (pH 7.0-11.0, optimum pH 8.0; 20-45°C, optimum 28°C) strains were isolated from root nodules of Sphaerophysa salsula and characterized by means of a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the four strains formed a new lineage related to the genus Rhizobium and the sequence similarities between the isolate and the most related type strain Rhizobium giardinii was 96.5%. These strains also formed a distinctive group from the reference strains for defined Rhizobium species based on housekeeping gene sequences (atpD and recA), BOX-PCR fingerprinting, phenotypic features and symbiotic properties. The representative strain CCNWGS0238(T) has DNA-DNA relatedness of less than 33.4% with the most closely related species R. giardinii. It is therefore proposed as a new species, Rhizobium sphaerophysae sp. nov., with isolate CCNWGS0238(T) (=ACCC17498(T) = HAMBI3074(T)) as the type strain.
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi, 712100, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Jian Feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Wei Min
authors.initials: WM
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Ming
authors.initials: M
authors.lastname: Tang
authors.forename: Ge Hong
authors.initials: GH
authors.lastname: Wei
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012231:Rhizobium / Q000235:genetics / Q000302:isolation & purification*; D053204:Root Nodules, Plant / Q000382:microbiology*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S