title: Serious complications in gene-expression studies with stress perturbation: An example of UV-exposed p53-mutant mouse embryonic fibroblasts.
abstract: Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose UV specific- and high-dose stress-related responses, which represent a plea for UV dose range-finding in experimental design.
authors.affiliation: MicroArray Department and Integrative Bioinformatics Unit (MAD-IBU); Swammerdam Institute for Life Sciences; Faculty of Science; University of Amsterdam (UvA); Amsterdam, The Netherlands.
authors.forename: Oskar
authors.initials: O
authors.lastname: Bruning
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Yuan
authors.forename: Wendy
authors.initials: W
authors.lastname: Rodenburg
authors.forename: Wendy
authors.initials: W
authors.lastname: Bruins
authors.forename: Conny T
authors.initials: CT
authors.lastname: van Oostrom
authors.forename: Han
authors.initials: H
authors.lastname: Rauwerda
authors.forename: Floyd Ra
authors.initials: FR
authors.lastname: Wittink
authors.forename: Martijs J
authors.initials: MJ
authors.lastname: Jonker
authors.forename: Annemieke
authors.initials: A
authors.lastname: de Vries
authors.forename: Timo M
authors.initials: TM
authors.lastname: Breit