title: N-terminal region of chitinase I of Bacillus circulans KA-304 contained new chitin-biding domain.
abstract: Chitinase I (CHI1) of Bacillus circulans KA-304 forms protoplasts from Schizophyllum commune mycelia when the enzyme is combined with α-1,3-glucanase of B. circulans KA-304. CHI1 consists of an N-terminal unknown region and a C-terminal catalytic region classified into the glycoside hydrolase family-19 type. An N-terminal region-truncated mutant of CHI 1 (CatCHI1), which was expressed in Escherichia coli Rosetta-gami B (DE3), lost colloidal chitin- and powder chitin-binding activities. The colloidal chitin- and the powder chitin-hydrolyzing activities of CatCHI1 were lower than those of CHI1, and CatCHI1 was not effective in forming the protoplast. A fusion protein of the N-terminal region of CHI1 and green fluorescent protein (Nterm-GFP) was expressed in E. coli, and the fusion protein was adsorbed to colloidal chitin, powder chitin, and chitosan. Fluorescence microscopy analysis showed that Nterm-GFP bound to the S. commune cell-wall.
authors.affiliation: Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University, Shiga, Japan.
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Yano
authors.forename: Wasana
authors.initials: W
authors.lastname: Suyotha
authors.forename: Arata
authors.initials: A
authors.lastname: Honda
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Takagi
authors.forename: Nopakarn
authors.initials: N
authors.lastname: Rattanakit-Chandet
authors.forename: Mamoru
authors.initials: M
authors.lastname: Wakayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Tachiki
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D017434:Protein Structure, Tertiary; D011523:Protoplasts / Q000378:metabolism; D012567:Schizophyllum / Q000166:cytology; D017384:Sequence Deletion
chemical_list: D002686:Chitin; D002688:Chitinases