title: Suppression of zinc finger protein 580 by high oxLDL/LDL-ratios is followed by enhanced expression of endothelial IL-8.
abstract: OBJECTIVE
  The Interleukin 8 (IL-8) response of endothelial cells to lipoproteins has well known implications for the development and progression of atherosclerosis. In this study we sought for the role of zinc finger protein 580 (ZNF580) in the endothelial IL-8 response to lipoproteins.
  
  
  METHODS
  In human umbilical vein endothelial cells (HUVEC) ZNF580 and IL-8 levels were examined by real-time-RT-PCR, immunoblotting and immunostaining or ELISA, respectively.
  
  
  RESULTS
  ZNF580 is located in the nucleus and regulated by LDL and HDL depending on the oxLDL/LDL-ratio but not by TNFα. IL-8 expression profiles are inversely influenced by the oxLDL/LDL-ratio, both in vitro and in vivo. Knock down of ZNF580 enhances the expression and release of IL-8 and increases monocyte arrest under flow conditions in vitro.
  
  
  CONCLUSIONS
  ZNF580 is a novel factor in the lipoprotein-dependent regulation of IL-8 and monocyte arrest. Therefore it may be a new potential target for intervention in atherosclerosis.
authors.affiliation: Department of Neurology, Charité-Universitaetsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany. christian.hoffmann@charite.de
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Hoffmann
authors.forename: Margret
authors.initials: M
authors.lastname: Hohberg
authors.forename: Sven
authors.initials: S
authors.lastname: Chlench
authors.forename: Julian
authors.initials: J
authors.lastname: Maroski
authors.forename: Marek
authors.initials: M
authors.lastname: Drab
authors.forename: Günter
authors.initials: G
authors.lastname: Siegel
authors.forename: Axel R
authors.initials: AR
authors.lastname: Pries
authors.forename: Andreas
authors.initials: A
authors.lastname: Zakrzewicz
mesh_terms: D050197:Atherosclerosis / Q000378:metabolism / Q000517:prevention & control; D015153:Blotting, Western; D002448:Cell Adhesion; D002478:Cells, Cultured; D018920:Coculture Techniques; D015536:Down-Regulation; D042783:Endothelial Cells / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; C550122:ZNF580 protein, human; C099252:oxidized low density lipoprotein