title: Nucleic acid binding behaviors and cytotoxic properties of a Ru(II) complex.
abstract: The interactions of complex [Ru(bpy)(2)(hnip)](2+) (1) {bpy = 2,2'-bipyridine, hnip = 2-(2-hydroxy-1-naphthyl)imidazo[4,5-f][1,10]phenanthroline} with calf thymus DNA and yeast tRNA were investigated by UV-vis spectroscopy, fluorescence spectroscopy, viscosity, equilibrium dialysis, and circular dichroism. In addition, the antitumor activities of complex 1 were evaluated with MTT method. These results indicate that the structures of DNA and RNA have significant effects on the binding behaviors of complex 1. Further, complex 1 demonstrates different antitumor activities against selected cancer cell lines in vitro.
authors.affiliation: Key Laboratory of Environment-Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan, PR China. lfwyxh@yahoo.com.cn
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Tan
authors.forename: Fu-Cang
authors.initials: FC
authors.lastname: Song
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Zou
authors.forename: Xi-Ling
authors.initials: XL
authors.lastname: Ling
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009696:Nucleic Acids / Q000378:metabolism*; D012331:RNA, Fungal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D013053:Spectrophotometry
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009696:Nucleic Acids; D012331:RNA, Fungal; D012428:Ruthenium; D004247:DNA; D012343:RNA, Transfer; C427203:calf thymus DNA