title: Sinapine detection in radish taproot using surface desorption atmospheric pressure chemical ionization mass spectrometry.
abstract: Plant research and natural product detection are of sustainable interests. Benefited by direct detection with no sample preparation, sinapine, a bioactive chemical usually found in various seeds of Brassica plants, has been unambiguously detected in radish taproot (Raphanus sativus) tissue using a liquid-assisted surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI-MS). A methanol aqueous solution (1:1) was nebulized by a nitrogen sheath gas toward the corona discharge, resulting in charged ambient small droplets, which affected the radish tissue for desorption/ionization of analytes on the tissue surface. Thus, sinapine was directly detected and identified by tandem DAPCI-MS experiments without sample pretreatment. The typical relative standard deviation (RSD) of this method for sinapine detection was 5-8% for six measurements (S/N=3). The dynamic response range was 10(-12)-10(-7) g/cm2 for sinapine on the radish skin surface. The discovery of sinapine in radish taproot was validated by using HPLC-UV methods. The data demonstrated that DAPCI assisted by solvent enhanced the overall efficiency of the desorption/ionization process, enabling sensitive detection of bioactive compounds in plant tissue.
authors.affiliation: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, College of Chemistry, Biology and Material Science, East China Institute of Technology, Nanchang, Jiangxi Province 330013, People's Republic of China.
authors.forename: Dejuan
authors.initials: D
authors.lastname: Huang
authors.forename: Liping
authors.initials: L
authors.lastname: Luo
authors.forename: Cuicui
authors.initials: C
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Zhou
authors.forename: Huanwen
authors.initials: H
authors.lastname: Chen
mesh_terms: D002794:Choline / Q000031:analogs & derivatives* / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D031224:Raphanus / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D010936:Plant Extracts; C005685:sinapine; D002794:Choline