title: Antibody arrays identify protein-protein interactions in chronic myeloid leukaemia.
abstract: Multiprotein complex formation with p210(BCR-ABL1) is likely to play a major role in determining cellular abnormalities in chronic myeloid leukaemia (CML). Although many p210(BCR-ABL1) binding partners have been identified, it is likely that many have not. We evaluated the use of co-immunoprecipitation and antibody arrays and found that this approach is capable of identifying new p210(BCR-ABL1) binding partners, and may contribute to the search for new therapeutic targets in CML.
authors.affiliation: Department of Haematology, Imperial College, Faculty of Medicine, Hammersmith Campus, Du Cane Road, London, UK.
authors.forename: Hetal
authors.initials: H
authors.lastname: Patel
authors.forename: Georgios
authors.initials: G
authors.lastname: Nteliopoulos
authors.forename: Zacharoula
authors.initials: Z
authors.lastname: Nikolakopoulou
authors.forename: Amanda
authors.initials: A
authors.lastname: Jackson
authors.forename: Myrtle Y
authors.initials: MY
authors.lastname: Gordon
mesh_terms: D000906:Antibodies / Q000276:immunology; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D047468:Immunoprecipitation; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D009363:Neoplasm Proteins; D016044:Fusion Proteins, bcr-abl