title: Effect of a mitochondria-targeted vitamin E derivative on mitochondrial alteration and systemic oxidative stress in mice.
abstract: The objective of the present study was to determine whether a mitochondria-targeted vitamin E derivative (MitoVit E) would affect certain mitochondrial parameters, as well as systemic oxidative stress. A total of sixty-four mice were fed a high-fat (HF) diet for 5 weeks. They were then switched to either a low-fat (LF) or a medium-fat (MF) diet, and administered orally with MitoVit E (40 mg MitoVit E/kg body weight) or drug vehicle (10 % (v/v) ethanol in 0·9 % (w/v) NaCl solution), every other day for 5 weeks. Mitochondrial ATP and H(2)O(2) production rates in both the liver and the gastrocnemius were not affected by MitoVit E administration in either LF or MF diet-fed mice. However, the number and average size of the subsarcolemmal mitochondria, but not the intermyofibrillar mitochondria, from the soleus muscle were significantly higher in the MF group receiving MitoVit E (MF-E) than in the MF group receiving vehicle only (MF-C). After the mice were switched from the HF diet to the four dietary treatments (LF-C, LF-E, MF-C and MF-E), the decrease in urinary isoprostane concentration was significantly greater in the LF-E group than in the other three groups during the whole study (weeks 6-10). In addition, MitoVit E significantly increased plasma superoxide dismutase (SOD) activity in the MF diet-fed group without affecting plasma glutathione peroxidase activity or H(2)O(2) levels. Overall, these data suggest that MitoVit E affects subsarcolemmal mitochondrial density and systemic oxidative stress parameters such as plasma SOD activity and urinary isoprostane concentration.
authors.affiliation: Interdepartmental Graduate Program in Nutritional Sciences, Iowa State University, Ames, IA 50011-3150, USA.
authors.forename: Gaowei
authors.initials: G
authors.lastname: Mao
authors.forename: George A
authors.initials: GA
authors.lastname: Kraus
authors.forename: Ikyon
authors.initials: I
authors.lastname: Kim
authors.forename: Michael E
authors.initials: ME
authors.lastname: Spurlock
authors.forename: Theodore B
authors.initials: TB
authors.lastname: Bailey
authors.forename: Donald C
authors.initials: DC
authors.lastname: Beitz
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D019587:Dietary Supplements; D016503:Drug Delivery Systems; D006861:Hydrogen Peroxide / Q000378:metabolism; D028421:Isoprostanes / Q000652:urine; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000187:drug effects* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D014451:Ubiquinone / Q000494:pharmacology
chemical_list: D028421:Isoprostanes; C478642:MitoVit E; D009943:Organophosphorus Compounds; D014451:Ubiquinone; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide