title: Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats.
abstract: OBJECTIVE
  The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats.
  
  
  METHODS
  In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (p<0.05).
  
  
  RESULTS
  In the histometric analysis, at 7, 15 and 30 days, Group T (0.72 ± 0.05 mm², 0.57 ± 0.14 mm², 0.62 ± 0.07 mm²), showed less bone loss (p<0.05) than Group C (1.35 ± 0.25 mm²; 1.40 ± 0.31 mm²; 1.29 ± 0.27 mm²), respectively.
  
  
  CONCLUSIONS
  Subgingival irrigation with TTC-HCL was an effective adjunctive treatment for periodontal disease induced in rats.
authors.affiliation: Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State Universit, Araçatuba, SP, Brazil. leandroataunesp@ig.com.br
authors.forename: Leandro Araújo
authors.initials: LA
authors.lastname: Fernandes
authors.forename: Thiago Marchi
authors.initials: TM
authors.lastname: Martins
authors.forename: Juliano Milanezi de
authors.initials: JM
authors.lastname: Almeida
authors.forename: Maria José Hitomi
authors.initials: MJ
authors.lastname: Nagata
authors.forename: Leticia Helena
authors.initials: LH
authors.lastname: Theodoro
authors.forename: Valdir Gouveia
authors.initials: VG
authors.lastname: Garcia
authors.forename: Alvaro Francisco
authors.initials: AF
authors.lastname: Bosco
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D012534:Dental Scaling; D008297:Male; D010518:Periodontitis / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D017208:Rats, Wistar; D016745:Root Planing; D013752:Tetracycline / Q000627:therapeutic use*; D007507:Therapeutic Irrigation; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline