title: An in vitro protocol for direct isolation of potential probiotic lactobacilli from raw bovine milk and traditional fermented milks.
abstract: A method for isolating potential probiotic lactobacilli directly from traditional milk-based foods was developed. The novel digestion/enrichment protocol was set up taking care to minimize the protective effect of milk proteins and fats and was validated testing three commercial fermented milks containing well-known probiotic Lactobacillus strains. Only probiotic bacteria claimed in the label were isolated from two out of three commercial fermented milks. The application of the new protocol to 15 raw milk samples and 6 traditional fermented milk samples made it feasible to isolate 11 potential probiotic Lactobacillus strains belonging to Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus plantarum, Lactobacillus reuteri, and Lactobacillus vaginalis species. Even though further analyses need to ascertain functional properties of these lactobacilli, the novel protocol set-up makes it feasible to isolate quickly potential probiotic strains from traditional milk-based foods reducing the amount of time required by traditional procedures that, in addition, do not allow to isolate microorganisms occurring as sub-dominant populations.
authors.affiliation: Institute of Sciences of Food Production, National Research Council of Italy, Via G. Amendola 122/o, 70126, Bari, Italy. federico.baruzzi@ispa.cnr.it
authors.forename: Federico
authors.initials: F
authors.lastname: Baruzzi
authors.forename: Palmiro
authors.initials: P
authors.lastname: Poltronieri
authors.forename: Grazia Marina
authors.initials: GM
authors.lastname: Quero
authors.forename: Maria
authors.initials: M
authors.lastname: Morea
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Morelli
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D043302:Cultured Milk Products / Q000378:metabolism / Q000382:microbiology; D005285:Fermentation; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008892:Milk / Q000378:metabolism / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D019936:Probiotics / Q000378:metabolism*