title: Ani s 10, a new Anisakis simplex allergen: cloning and heterologous expression.
abstract: Anisakiasis is a human disease caused by accidental ingestion of larval nematodes belonging to the Anisakidae family. Anisakiasis is often associated with a strong allergic response. Diagnosis of A. simplex allergy is currently carried out by test based on the IgE reactivity to a complete extract of L3 Anisakis larvae although the specificity of these diagnostic tests is poor. Improving the specificity of the diagnostic test is possible using purified recombinant allergens. A new Anisakis allergen, named Ani s 10, was detected by immunoscreening an expression cDNA library constructed from L3 Anisakis simplex larvae. The new allergen was overproduced in Escherichia coli; it is a protein of 212 amino acids and it was localized as a 22 kDa protein band in an ethanol fractionated extract from the parasite. Ani s 10 has no homology with any other described protein, and its sequence is composed by seven almost identical repetitions of 29 amino acids each. A total of 30 of 77 Anisakis allergic patients (39%) were positive both to rAni s 10 and natural Ani s 10 by immunoblotting. The new allergen could be useful in a component-resolved diagnosis system for Anisakis allergy.
authors.affiliation: Servicio de Inmunologia, Hospital Carlos III, Madrid, Spain.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Caballero
authors.forename: Ana
authors.initials: A
authors.lastname: Umpierrez
authors.forename: Ignacio
authors.initials: I
authors.lastname: Moneo
authors.forename: Rosa
authors.initials: R
authors.lastname: Rodriguez-Perez
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D017129:Anisakiasis / Q000276:immunology; D017163:Anisakis / Q000235:genetics* / Q000276:immunology; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D018509:DNA, Helminth / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000469:parasitology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D007073:Immunoglobulin E