title: Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM.
abstract: OBJECTIVE
  To optimize the medium components for the production of indole-3-acetic acid (IAA) by isolated bacterium Pantoea agglomerans strain PVM.
  
  
  RESULTS
  Present study deals with the production of an essential plant hormone IAA by a bacterial isolate P. agglomerans strain PVM identified by 16S rRNA gene sequence analysis. The medium containing 8g l(-1) of meat extract and 1g l(-1) of l-tryptophan (precursor) at optimum pH 7, 30°C and 48-h incubation gave the maximum production of IAA (2·191 g l(-1) ). Effect of IAA synthesized on in vitro root induction in Nicotiana tobacum (leaf) explants was compared with that of control. IAA was characterized by high-performance thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectroscopy.
  
  
  CONCLUSIONS
  Pantoea agglomerans strain PVM was a good candidate for the inexpensive and utmost production of IAA in short period, as it requires simple medium (meat extract and l-tryptophan).
  
  
  CONCLUSIONS
  The present report first time showed the rapid, cost-effective and maximum production of IAA. No reports are available on the optimization of particular medium components for the production of IAA. This study demonstrates a novel approach for in vitro root induction in N. tobacum (leaf) explants.
authors.affiliation: Department of Biotechnology Shivaji University, Kolhapur, India.
authors.forename: O A
authors.initials: OA
authors.lastname: Apine
authors.forename: J P
authors.initials: JP
authors.lastname: Jadhav
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000378:metabolism*; D007218:Industrial Microbiology; D020636:Pantoea / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000096:biosynthesis; D018517:Plant Roots / Q000254:growth & development; D014026:Nicotiana / Q000254:growth & development; D014364:Tryptophan / Q000737:chemistry
chemical_list: D003470:Culture Media; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D014364:Tryptophan