title: Receptor specificity of subtype H1 influenza A viruses isolated from swine and humans in the United States.
abstract: The evolution of classical swine influenza viruses receptor specificity preceding the emergence of the 2009 H1N1 pandemic virus was analyzed in glycan microarrays. Classical swine influenza viruses from the α, β, and γ antigenic clusters isolated between 1945 and 2009 revealed a binding profile very similar to that of 2009 pandemic H1N1 viruses, with selectivity for α2-6-linked sialosides and very limited binding to α2-3 sialosides. Despite considerable genetic divergence, the 'human-like' H1N1 viruses circulating in swine retained strong binding preference for α2-6 sialylated glycans. Interspecies transmission of H1N1 influenza viruses from swine to humans or from humans to swine has not driven selection of viruses with distinct novel receptor binding specificities. Classical swine and human seasonal H1N1 influenza viruses have conserved specificity for similar α2-6-sialoside receptors in spite of long term circulation in separate hosts, suggesting that humans and swine impose analogous selection pressures on the evolution of receptor binding function.
authors.affiliation: Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Chen
authors.forename: Pierre
authors.initials: P
authors.lastname: Rivailler
authors.forename: Jaber
authors.initials: J
authors.lastname: Hossain
authors.forename: Paul
authors.initials: P
authors.lastname: Carney
authors.forename: Amanda
authors.initials: A
authors.lastname: Balish
authors.forename: Ijeoma
authors.initials: I
authors.lastname: Perry
authors.forename: C Todd
authors.initials: CT
authors.lastname: Davis
authors.forename: Rebecca
authors.initials: R
authors.lastname: Garten
authors.forename: Bo
authors.initials: B
authors.lastname: Shu
authors.forename: Xiyan
authors.initials: X
authors.lastname: Xu
authors.forename: Alexander
authors.initials: A
authors.lastname: Klimov
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.forename: Nancy J
authors.initials: NJ
authors.lastname: Cox
authors.forename: Sabrina
authors.initials: S
authors.lastname: Swenson
authors.forename: James
authors.initials: J
authors.lastname: Stevens
authors.forename: Amy
authors.initials: A
authors.lastname: Vincent
authors.forename: Marie
authors.initials: M
authors.lastname: Gramer
authors.forename: Ruben O
authors.initials: RO
authors.lastname: Donis
mesh_terms: D000818:Animals; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification / Q000502:physiology*; D007251:Influenza, Human / Q000821:virology*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D009976:Orthomyxoviridae Infections / Q000821:virology*; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014481:United States; D053585:Virus Attachment
chemical_list: D011991:Receptors, Virus; D019158:N-Acetylneuraminic Acid