title: Method for the direct determination of available carbohydrates in low-carbohydrate products using high-performance anion exchange chromatography.
abstract: An improved method for direct determination of available carbohydrates in low-level products has been developed and validated for a low-carbohydrate soy infant formula. The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0% across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4%, respectively. Overall average recovery was 99.4% (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106% (n = 10). It is expected that the method could be applied to a variety of low-carbohydrate foods and beverages.
authors.affiliation: Covance Laboratories, 3301 Kinsman Blvd, Madison, WI 53704-2523, USA. david.ellingson@covance.com
authors.forename: David
authors.initials: D
authors.lastname: Ellingson
authors.forename: Brian
authors.initials: B
authors.lastname: Potts
authors.forename: Phillip
authors.initials: P
authors.lastname: Anderson
authors.forename: Greg
authors.initials: G
authors.lastname: Burkhardt
authors.forename: Wayne
authors.initials: W
authors.lastname: Ellefson
authors.forename: Darryl
authors.initials: D
authors.lastname: Sullivan
authors.forename: Wesley
authors.initials: W
authors.lastname: Jacobs
authors.forename: Robert
authors.initials: R
authors.lastname: Ragan
mesh_terms: D000837:Anion Exchange Resins; D002138:Calibration; D002852:Chromatography, Ion Exchange; D004040:Dietary Carbohydrates / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013213:Starch / Q000032:analysis
chemical_list: D000837:Anion Exchange Resins; D004040:Dietary Carbohydrates; D007202:Indicators and Reagents; D012996:Solutions; D013213:Starch