title: Cord blood monocyte subsets are similar to adult and show potent peptidoglycan-stimulated cytokine responses.
abstract: Human monocytes can be divided into two major subpopulations, CD14(++) CD16(-) and CD14(+) CD16(+) cells, which are suggested to play different roles in antimicrobial responses. In neonates, characteristics and functional responses of monocyte subsets have not previously been explored, and might contribute to the qualitative difference between neonatal and adult cytokine profiles. We report that at baseline, monocyte subsets in cord blood and adult peripheral blood are present in similar frequencies, and show similar expression of CD11c, CD80/CD86, CD163 and HLA-DR. In response to the bacterial ligand peptidoglycan, cord blood monocytes had high inherent capacity for production of the early-response cytokines with levels of tumour necrosis factor and interleukin-12p70 exceeding adult levels, and also a higher phosphorylation of p38-mitogen-activated protein kinase. The CD14(+) CD16(+) cells expressed more interleukin-12p70 than CD14(++) CD16(-) cells and were present in a higher frequency in peptidoglycan-stimulated cord blood mononuclear cell cultures. Together, the behaviour of cord blood CD14(+) CD16(+) cells following peptidoglycan stimulation might indicate a qualitative difference between the neonatal antimicrobial response and that of the adult. In addition we found that serum factors in cord blood and adult sera affected cytokine production similarly, with the exception of tumour necrosis factor, regardless of the source of serum or cells. Overall, our data provide new insights into monocyte heterogeneity in cord blood and monocyte subset responses to a bacterial ligand at birth.
authors.affiliation: Department of Immunology, Wenner-Gren Institute, Stockholm University, Stockholm, Sweden. ebba.sohlberg@imun.su.se
authors.forename: Ebba
authors.initials: E
authors.lastname: Sohlberg
authors.forename: Shanie
authors.initials: S
authors.lastname: Saghafian-Hedengren
authors.forename: Katarina
authors.initials: K
authors.lastname: Bremme
authors.forename: Eva
authors.initials: E
authors.lastname: Sverremark-Ekström
mesh_terms: D000328:Adult; D002469:Cell Separation; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007231:Infant, Newborn / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000276:immunology; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D010457:Peptidoglycan / Q000276:immunology; D017452:Receptors, IgG / Q000096:biosynthesis / Q000276:immunology
chemical_list: D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D010457:Peptidoglycan; D017452:Receptors, IgG