title: Comparative study of human hematopoietic cell engraftment into BALB/c and C57BL/6 strain of rag-2/jak3 double-deficient mice.
abstract: Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2⁻/⁻Jak3⁻/⁻) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34+ hematopoietic stem cells were successfully engrafted into Balb/c Rag-2⁻/⁻Jak3⁻/⁻ mice; however, the engraftment rate was far lower in C57/BL6 Rag-2⁻/⁻Jak3⁻/⁻ mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment.
authors.affiliation: Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Honjo 2-2-1, Kumamoto 860-0811, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Ono
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Hattori
authors.forename: Ryusho
authors.initials: R
authors.lastname: Kariya
authors.forename: Sumako
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Manabu
authors.initials: M
authors.lastname: Taura
authors.forename: Hideki
authors.initials: H
authors.lastname: Harada
authors.forename: Shinya
authors.initials: S
authors.lastname: Suzu
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis / Q000276:immunology; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics* / Q000276:immunology; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D053616:Janus Kinase 3 / Q000172:deficiency* / Q000235:genetics* / Q000276:immunology; D020014:K562 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D018952:Antigens, CD34; D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; C507935:Jak3 protein, mouse; D053616:Janus Kinase 3