title: The influence of surface energy of titanium-zirconium alloy on osteoblast cell functions in vitro.
abstract: The success of an implant used for bone regeneration and repair is determined by the events that take place at the cell-material interface. An understanding of these interactions in vitro gives insights into the formulation of ideal conditions for their effective functioning in vivo. Thus, it is not only important to understand the physico-chemical properties of the materials but, also necessary to assess the cellular responses to them to determine their long-term stability and efficacy as implants. In the present study, we have compared the physico-chemical and biological properties of titanium (Ti) and two Ti-based alloys, namely: Ti- Zirconium (TiZr) and Ti-Niobium (TiNb). The morphology, chemical analysis, surface roughness, and contact angle measurements of the alloys were assessed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), profilometer, and contact angle goniometer, respectively whereas the biological properties of the materials were evaluated by measuring the adhesion, proliferation, and differentiation of MC3T3-E1 osteoblast cells on the surfaces of these alloys. Our results indicate that the biological properties of osteoblasts were better on TiZr surface than on TiNb surface. Furthermore, the surface energy and substrate composition influenced the superior biological activity of the TiZr alloy.
authors.affiliation: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India; Institute for Technology Research and Innovation, Deakin University, Pigdons Road, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Subhash
authors.initials: S
authors.lastname: Sista
authors.forename: Cui'e
authors.initials: C
authors.lastname: Wen
authors.forename: Peter D
authors.initials: PD
authors.lastname: Hodgson
authors.forename: Gopal
authors.initials: G
authors.lastname: Pande
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000497:Alloys / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D013499:Surface Properties; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D000497:Alloys; C000599177:Ti-Zr alloy; D014867:Water; D015675:Osteocalcin; D000469:Alkaline Phosphatase