title: Sensitive voltammetric determination of baicalein at DNA Langmuir-Blodgett film modified glassy carbon electrode.
abstract: The present paper describes to modify a double stranded DNA-octadecylamine (ODA) Langmuir-Blodgett film on a glassy carbon electrode (GCE) surface to develop a voltammetric sensor for the detection of trace amounts of baicalein. The electrode was characterized by atomic force microscopy (AFM) and cyclic voltammetry (CV). Electrochemical behaviour of baicalein at the modified electrode had been investigated in pH 2.87 Britton-Robinson buffer solutions by CV and square wave voltammetry (SWV). Compared with bare GCE, the electrode presented an electrocatalytic redox for baicalein. Under the optimum conditions, the modified electrode showed a linear voltammetric response for the baicalein within a concentration range of 1.0 × 10(-8)-2.0 × 10(-6) mol L(-1), and a value of 6.0 × 10(-9) mol L(-1) was calculated for the detection limit. And the modified electrode exhibited an excellent immunity from epinephrine, dopamine, glucose and ascorbic acid interference. The method was also applied successfully to detect baicalein in the medicinal tablets and spiked human blood serum samples with satisfactory results.
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhao
authors.forename: Yanzheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huige
authors.initials: H
authors.lastname: Yang
authors.forename: Baoxian
authors.initials: B
authors.lastname: Ye
mesh_terms: D000588:Amines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry; D044950:Flavanones / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000737:chemistry; D015203:Reproducibility of Results; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000588:Amines; D004573:Electrolytes; D044950:Flavanones; D004364:Pharmaceutical Preparations; C006680:baicalein; D002244:Carbon; D004247:DNA; C009317:stearylamine