title: Integrated systems for rapid point of care (PoC) blood cell analysis.
abstract: Counting the different subpopulations of cells in a fingerprick of human blood is important for a number of clinical point-of-care (PoC) applications. It is a challenge to demonstrate the integration of sample preparation and detection techniques in a single platform. In this paper we demonstrate a generic microfluidic platform that combines sample processing and characterisation and enumeration in a single, integrated system. Results of microfluidic 3-part differential leukocyte (granulocyte, lymphocyte and monocyte) counts, together with erythrocyte and thrombocyte (platelet) counts, in human blood are shown and corroborated with results from hospital clinical laboratory analysis.
authors.affiliation: Philips Research Laboratories, 101 Cambridge Science Park, Milton Road, Cambridge CB4 0FY, UK.
authors.forename: Cees
authors.initials: C
authors.lastname: van Berkel
authors.forename: James D
authors.initials: JD
authors.lastname: Gwyer
authors.forename: Steve
authors.initials: S
authors.lastname: Deane
authors.forename: Nicolas G
authors.initials: NG
authors.lastname: Green
authors.forename: Nicolas
authors.initials: N
authors.lastname: Green
authors.forename: Judith
authors.initials: J
authors.lastname: Holloway
authors.forename: Veronica
authors.initials: V
authors.lastname: Hollis
authors.forename: Hywel
authors.initials: H
authors.lastname: Morgan
mesh_terms: D053000:Analytic Sample Preparation Methods; D001772:Blood Cell Count / Q000379:methods*; D001773:Blood Cells / Q000166:cytology*; D017097:Electric Impedance; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D019095:Point-of-Care Systems; D012815:Signal Processing, Computer-Assisted; D018511:Systems Integration; D013997:Time Factors