title: Human skin penetration of gold nanoparticles through intact and damaged skin.
abstract: Gold nanoparticles (AuNPs) are produced for many applications but there is a lack of available data on their skin absorption. Experiments were performed using the Franz diffusion cell method with intact and damaged human skin. A physiological solution was used as receiving phase and 0.5 mL (1st exp) and 1.5 mL (2nd exp) of a solution containing 100 mg L⁻¹ of AuNPs (15 and 45 μg cm⁻², respectively) was applied as donor phase to the outer surface of the skin for 24 h. Skin absorption was dose dependent. Mean gold content of 214.0 ± 43.7 ng cm⁻² and 187.7 ± 50.2 ng cm⁻² were found in the receiving solutions of cells where the AuNPs solution was applied in higher concentration on intact skin (8 Franz cells) and on damaged skin (8 Franz cells), respectively. Twenty-four hours gold flux permeation was 7.8 ± 2.0 ng cm⁻² h⁻¹ and 7.1 ± 2.5 ng cm⁻² h⁻¹ in intact and damaged skin, respectively, with a lag time less than 1 hour. Transmission Electron Microscope analysis on skin samples and chemical analysis using Inductively Coupled Plasma-Mass Spectrometry demonstrated the presence of AuNPs into epidermis and dermis. This study showed that AuNPs are able to penetrate the human skin in an in vitro diffusion cell system.
authors.affiliation: UCO Medicina del Lavoro, Dipartimento di Scienze di Medicina Pubblica, Università di Trieste, Trieste, Italy. larese@units.it
authors.forename: Francesca Larese
authors.initials: FL
authors.lastname: Filon
authors.forename: Matteo
authors.initials: M
authors.lastname: Crosera
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Adami
authors.forename: Massimo
authors.initials: M
authors.lastname: Bovenzi
authors.forename: Federica
authors.initials: F
authors.lastname: Rossi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maina
mesh_terms: D000005:Abdomen; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods; D020405:Dermis; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D004817:Epidermis; D006046:Gold / Q000032:analysis / Q000493:pharmacokinetics*; D006651:Histocytochemistry; D006801:Humans; D066298:In Vitro Techniques; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012867:Skin / Q000737:chemistry / Q000293:injuries / Q000378:metabolism*; D012869:Skin Absorption / Q000502:physiology*
chemical_list: D006046:Gold