title: [Effects of pretreatment regimen on mice liver injury in hematopoietic stem cell transplantation].
abstract: OBJECTIVE
  To investigate the effects of conditioning regimen on mice liver injury during hematopoietic stem cell transplantation.
  
  
  METHODS
  Forty healthy 8-10 weeks old female BALB/c mice were randomly divided into control group, non-myeloablative total body irradiation group, myeloablative total body irradiation group, busulfan group and cyclophosphamide group with 8 mice each. The general condition of each mouse was continuously observed. Peripheral white blood cells were counted and pathological changes of liver were examined by hematoxylin and eosin stain under microscope. The ultrastructure changes of hepatocyte and liver vascular endothelium were detected under transmission electron microscope.
  
  
  RESULTS
  Compared to control group, each experimental group showed that the count of white blood cells was decreased significantly after conditioning pretreatments (P < 0.05). The conditioning regimen had injury effects on the mice liver in each experimental group. There were various degrees of damage to the hepatocyte and liver vascular endothelium.
  
  
  CONCLUSIONS
  Total body irradiation, Busulfan and Cyclophosphamide all led to the damage of liver vascular endothelium in hematopoietic stem cell transplantation, and may be the important triggers of hepatic veno-occlusive disease.
authors.affiliation: Laboratory of Transplant Immunology, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Jia
authors.forename: Zhi-ling
authors.initials: ZL
authors.lastname: Yan
authors.forename: Shi-juan
authors.initials: SJ
authors.lastname: Xu
authors.forename: Kai-lin
authors.initials: KL
authors.lastname: Xu
authors.forename: Ling-yu
authors.initials: LY
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002066:Busulfan / Q000009:adverse effects; D003520:Cyclophosphamide / Q000009:adverse effects; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006504:Hepatic Veno-Occlusive Disease / Q000209:etiology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D019172:Transplantation Conditioning / Q000009:adverse effects*; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D003520:Cyclophosphamide; D002066:Busulfan