title: [Detection of methylation and deletion of p16 gene in non-small cell lung cancer].
abstract: BACKGROUND
  To investigate the methylation and deletion of p16 gene and its diagnostic value in non-small cell lung cancer.
  
  
  METHODS
  A total of 50 lung cancer tissues and 54 normal lung tissues were examined for p16 gene methylation in exon 1 and deletion in exon 2 by PCR based methylation analysis and duplex PCR respectively.
  
  
  RESULTS
  Out of 50 lung cancer tissues, 16 were positive for the p16 gene exon 1 methylation (32.0%), and 14 for the p16 gene exon 2 deletion (28.0%). However, in 54 cases of normal lung tissues, only 2 showed the p16 gene exon 1 methylation(3.7%), and none showed the p16 gene exon 2 deletion. There were significant differences in methylation rate (Fisher's exact= 0.000 ) and deletion rate (Fisher's exact= 0.000) between the two groups.
  
  
  CONCLUSIONS
  The methylation and deletion may be important mechanisms for p16 gene inactivation in non-small cell lung cancer. The detection of p16 gene status may contribute to the diagnosis of lung cancer.
authors.affiliation: Department of Radiology, The Affiliated Hospital, Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R.China.
authors.forename: Mengqing
authors.initials: M
authors.lastname: Peng
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Delin
authors.initials: D
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Hao