title: Antiinflammatory constituents from Eclipta prostrata using RAW264.7 macrophage cells.
abstract: The whole plant extract of Eclipta prostrata and its isolated compounds were tested for their antiinflammatory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E2 (PGE2 ) and tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells, as well as for the antiinflammatory mechanism of the active compound on mRNA expression. Among the isolated compounds, orobol (5) exhibited the highest activity against NO release with an IC50 value of 4.6 μm, followed by compounds 1, 2 and 4 with IC50 values of 12.7, 14.9 and 19.1 μm, respectively. The IC50 value of compound 5 against PGE2 release was found to be 49.6 μm, whereas it was inactive towards TNF-α (IC50  > 100 μm). The mechanism of orobol (5) was found to down-regulate iNOS and COX-2 mRNA expression in a concentration-dependent manner. The present study may support the traditional use of Eclipta prostrata for the treatment of inflammatory-related diseases.
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand. supinyat@yahoo.com.
authors.forename: Supinya
authors.initials: S
authors.lastname: Tewtrakul
authors.forename: Sanan
authors.initials: S
authors.lastname: Subhadhirasakul
authors.forename: Pimpimon
authors.initials: P
authors.lastname: Tansakul
authors.forename: Sarot
authors.initials: S
authors.lastname: Cheenpracha
authors.forename: Chatchanok
authors.initials: C
authors.lastname: Karalai
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D036601:Eclipta / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone; C059811:orobol