title: Skeletal muscle electron transport chain dysfunction after sepsis in rats.
abstract: BACKGROUND
  The derangement in oxygen utilization occurring during sepsis is likely to be linked to impaired mitochondrial functioning. Skeletal muscle comprises 50%-60% of body cell mass and represents the largest organ potentially affected by systemic inflammation. Thus, we investigated whether sepsis induced by cecal ligation and puncture (CLP) modifies mitochondrial activity in respiratory and nonrespiratory skeletal muscle.
  
  
  METHODS
  Wistar rats were subjected to CLP and at different times, diaphragm and quadriceps were removed for the determination of electron transfer chain activities and mitochondrial oxidative stress. In addition, we determined diaphragm contractile strength.
  
  
  RESULTS
  In the quadriceps, 12 h after CLP we demonstrated a significant diminution on complex II-III activity. At late times (48 h after CLP), we demonstrated a decrease in the activity of all electron transfer chain complexes, which seemed to be secondary to early oxidative stress and correlates with diaphragm contractile strength. Differently from diaphragm, electron transfer chain was not decreased after sepsis and even oxidative stress was not increased at all times tested.
  
  
  CONCLUSIONS
  Our results suggest that quadriceps mitochondria are more resistant to sepsis-induced dysfunction.
authors.affiliation: Laboratório de Fisiopatologia Experimental, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.
authors.forename: Bruno B
authors.initials: BB
authors.lastname: Peruchi
authors.forename: Fabrícia
authors.initials: F
authors.lastname: Petronilho
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Rojas
authors.forename: Larissa
authors.initials: L
authors.lastname: Constantino
authors.forename: Francielle
authors.initials: F
authors.lastname: Mina
authors.forename: Francieli
authors.initials: F
authors.lastname: Vuolo
authors.forename: Mariane R
authors.initials: MR
authors.lastname: Cardoso
authors.forename: Cinara L
authors.initials: CL
authors.lastname: Gonçalves
authors.forename: Gislaine T
authors.initials: GT
authors.lastname: Rezin
authors.forename: Emílio L
authors.initials: EL
authors.lastname: Streck
authors.forename: Felipe
authors.initials: F
authors.lastname: Dal-Pizzol
mesh_terms: D000818:Animals; D002432:Cecum / Q000601:surgery; D004195:Disease Models, Animal; D042963:Electron Transport Complex II / Q000502:physiology*; D014450:Electron Transport Complex III / Q000502:physiology*; D008026:Ligation / Q000009:adverse effects; D008297:Male; D008931:Mitochondria, Muscle / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000503:physiopathology*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000503:physiopathology*
chemical_list: D042963:Electron Transport Complex II; D014450:Electron Transport Complex III