title: Genetic and neutralization sensitivity of diverse HIV-1 env clones from chronically infected patients in China.
abstract: As HIV-1 continues to spread in China from traditional high risk populations to the general public, its genetic makeup has become increasingly complex. However, the impact of these genetic changes on the biological and neutralization sensitivity of the virus is unknown. The current study aims to characterize the genetic, biological, and neutralization sensitivity of HIV-1 identified in China between 2004 and 2007. Based on a total of 107 full-length envelope genes obtained directly from the infected patients, we found that those viruses fell into three major genetic groups: CRF01_AE, subtype B', and subtype C/CRF07_BC/CRF08_BC/B'C. Pseudotyped viruses built upon the viable env genes have demonstrated their substantial variability in mediating viral entry and in sensitivity to neutralization by subtype-specific plasma pools and broadly neutralizing monoclonal antibodies (bnmAb). Many viruses are resistant to one or more bnmAb, including those known to have high potency against diverse viruses from outside China. Sequence and structural analysis has revealed several mechanisms by which these resistant viruses escape recognition from bnmAb. We believe that these results will help us to better understand the impact of genetic diversity on the neutralizing sensitivity of the viruses and to facilitate the design of immunogens capable of eliciting antibodies with potency and breadth similar to those of bnmAb.
authors.affiliation: Key Laboratory of AIDS Immunology of Ministry of Health, Department of Laboratory Medicine, No. 1 Hospital of China Medical University, Shenyang 110001, China. hongshang100@hotmail.com
authors.forename: Hong
authors.initials: H
authors.lastname: Shang
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Han
authors.forename: Xuanling
authors.initials: X
authors.lastname: Shi
authors.forename: Teng
authors.initials: T
authors.lastname: Zuo
authors.forename: Mark
authors.initials: M
authors.lastname: Goldin
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Xinquan
authors.initials: X
authors.lastname: Wang
authors.forename: Linqi
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D057134:Antibodies, Neutralizing / Q000737:chemistry; D002681:China; D002908:Chronic Disease; D018604:Epitope Mapping; D005260:Female; D015752:Genes, env / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017386:Sequence Homology, Amino Acid
chemical_list: D057134:Antibodies, Neutralizing