title: Diversity and metabolic impact of intestinal Lactobacillus species in healthy adults and the elderly.
abstract: The present study aimed at assessing the counts and species distribution of intestinal lactobacilli and exploring if the data are associated with BMI and blood glucose level in healthy adults and elderly persons. The BMI (P < 0·01), the level of fasting blood glucose (P < 0·001) and the total counts of lactobacilli (P < 0·01 by bacteriology; P < 0·001 by real-time PCR) were higher in the elderly. The number of species in adults was lower (P < 0·05), who were more often colonised with Lactobacillus acidophilus (P = 0·031) and L. helveticus (P < 0·001). In contrast, L. plantarum (P = 0·035), L. paracasei (P < 0·001) and L. reuteri (P = 0·031) were more prevalent in the elderly. L. rhamnosus was detected in adults (P < 0·001), but not in any elderly person. BMI was associated with counts of lactobacilli, adjusted for age and sex (P = 0·008). The higher BMI in both groups of persons was associated with the presence of obligate homofermentative lactobacilli and L. sakei, both adjusted for age and sex. Plasma glucose values were positively correlated with BMI and negatively correlated with colonisation with L. paracasei (P = 0·0238) in adults and on the borderline with L. fermentum (P = 0·052) in the elderly. Thus, the species-specific PCR analysis of Lactobacillus sp. combined with viable plating data indicates substantial age-related structural differences in the intestinal lactobacilli communities. The higher counts of intestinal Lactobacillus sp. are associated with higher BMI and blood glucose content, while their specific fermentative groups and species of lactobacilli appear at different glucose levels both in adults and in the elderly.
authors.affiliation: Department of Microbiology, Medical Faculty, University of Tartu, Tartu 50411, Estonia. jelena.stsepetova@ut.ee
authors.forename: Jelena
authors.initials: J
authors.lastname: Štšepetova
authors.forename: Epp
authors.initials: E
authors.lastname: Sepp
authors.forename: Helgi
authors.initials: H
authors.lastname: Kolk
authors.forename: Krista
authors.initials: K
authors.lastname: Lõivukene
authors.forename: Epp
authors.initials: E
authors.lastname: Songisepp
authors.forename: Marika
authors.initials: M
authors.lastname: Mikelsaar
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D015169:Colony Count, Microbial; D004957:Estonia; D005243:Feces / Q000382:microbiology; D005260:Female; D005285:Fermentation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D057230:Limit of Detection; D008297:Male; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D019936:Probiotics; D055815:Young Adult
chemical_list: D001786:Blood Glucose