title: Production of anti-amyloid β antibodies in mice fed rice expressing amyloid β.
abstract: The main signs of Alzheimer's disease (AD) are cognitive impairment and senile plaques composed of amyloid beta (Aβ) observed in patients' brains. Therefore, therapy for AD focuses on the removal of Aβ. We developed an "edible vaccine" that employs intestinal immunity with little to no side effects. Rice was utilized as an edible vaccine. It expressed GFP-Aβ42. Aβ rice was administered orally to wild-type (WT) mice causing production of anti-Aβ antibodies. Since Aβ rice was mixed with the cholera toxin B subunit (CTB), antibody against the rice seed protein was also produced. Then, mice were caused to develop immune tolerance against the rice seed protein by oral administration of Aβ rice mixed with CTB. The results indicated that only anti-Aβ antibodies were produced.
authors.affiliation: Department of Life Science, Graduate School of Arts and Science, The University of Tokyo, Tokyo, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Nojima
authors.forename: Rika
authors.initials: R
authors.lastname: Ishii-Katsuno
authors.forename: Eugene
authors.initials: E
authors.lastname: Futai
authors.forename: Noboru
authors.initials: N
authors.lastname: Sasagawa
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Taiji
authors.initials: T
authors.lastname: Yoshida
authors.forename: Shoichi
authors.initials: S
authors.lastname: Ishiura
mesh_terms: D000284:Administration, Oral; D000544:Alzheimer Disease / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D016229:Amyloid beta-Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D018604:Epitope Mapping; D005260:Female; D015870:Gene Expression; D008297:Male; D051379:Mice; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D018418:Th2 Cells / Q000276:immunology; D022462:Vaccines, Edible / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D016229:Amyloid beta-Peptides; D000906:Antibodies; D022462:Vaccines, Edible