title: An electrochemical biosensor for fructosyl valine for glycosylated hemoglobin detection based on core-shell magnetic bionanoparticles modified gold electrode.
abstract: A high-performance amperometric fructosyl valine (FV) biosensor was developed, based on immobilization of fructosyl amino-acid oxidase (FAO) on core-shell magnetic bionanoparticles modified gold electrode. Chitosan was used to introduce amino groups onto the surface of core-shell magnetic bionanoparticles (MNPs). With FAO as an enzyme model, a new fructosyl valine biosensor was fabricated. The biosensor showed optimum response, when operated at 50 mVs(-1) in 0.1M potassium phosphate buffer, pH 7.5 and 35°C. The biosensor exhibited excellent sensitivity [the detection limit is down to 0.1mM for FV], fast response time (less than 4s), wide linear range (from 0 to 2mM). Analytical recovery of added FV was 95.00-98.50%. Within batch and between batch coefficients of variation were <2.58% and <5.63%, respectively. The enzyme electrode was used 250 times over 3 months, when stored at 4°C.
authors.affiliation: Department of Biochemistry, M.D. University, Rohtak, Haryana 124 001, India.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Chawla
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006442:Glycated Hemoglobin / Q000032:analysis*; D006046:Gold; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D014633:Valine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D006442:Glycated Hemoglobin; D006046:Gold; D058185:Magnetite Nanoparticles; D014633:Valine; C045420:fructosylvaline