title: Comparison of cytotoxicity induced by 17α-ethynylestradiol and diethylstilbestrol in fish CCO and mammalian CHO-K1 cell lines.
abstract: The effects of synthetic estrogens 17α-ethynylestradiol (EE2) and diethylstilbestrol (DES) were compared on cell proliferation and morphology in Channel Catfish Ovary (CCO) and Chinese Hamster Ovary (CHO-K1) cells. EE2 exposure (0.1 and 0.5 μg/mL) induced stimulatory effect on CCO and CHO-K1 cell proliferation, while higher concentrations (1-10 μg/mL) showed cytotoxic effects. Increase in DES concentrations mainly resulted in dose-depended increase in cytotoxicity in both cell lines. Morphological changes induced by EE2 and DES exposure (5 μg/mL) showed disrupted cell monolayer and increased number and size of lysosomes. Comparison of IC(50) values showed almost equal sensitivity towards cytotoxicity of tested compounds in CCO and CHO-K1 cells.
authors.affiliation: Laboratory of Cell Culture Technology and Biotransformation, Faculty of Food Technology and Biotechnology, University of Zagreb, 6 Pierotti St, 10000 Zagreb, Croatia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Radošević
authors.forename: Tea
authors.initials: T
authors.lastname: Tonković
authors.forename: Igor
authors.initials: I
authors.lastname: Slivac
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Kniewald
authors.forename: Višnja
authors.initials: V
authors.lastname: Gaurina Srček
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003603:Cytotoxins / Q000633:toxicity*; D004054:Diethylstilbestrol / Q000633:toxicity*; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D010053:Ovary / Q000187:drug effects
chemical_list: D003603:Cytotoxins; D004967:Estrogens; D004997:Ethinyl Estradiol; D004054:Diethylstilbestrol