title: Acerogenin A, a natural compound isolated from Acer nikoense Maxim, stimulates osteoblast differentiation through bone morphogenetic protein action.
abstract: We investigated the effects of acerogenin A, a natural compound isolated from Acer nikoense Maxim, on osteoblast differentiation by using osteoblastic cells. Acerogenin A stimulated the cell proliferation of MC3T3-E1 osteoblastic cells and RD-C6 osteoblastic cells (Runx2-deficient cell line). It also increased alkaline phosphatase activity in MC3T3-E1 and RD-C6 cells and calvarial osteoblastic cells isolated from the calvariae of newborn mice. Acerogenin A also increased the expression of mRNAs related to osteoblast differentiation, including Osteocalcin, Osterix and Runx2 in MC3T3-E1 cells and primary osteoblasts: it also stimulated Osteocalcin and Osterix mRNA expression in RD-C6 cells. The acerogenin A treatment for 3days increased Bmp-2, Bmp-4, and Bmp-7 mRNA expression levels in MC3T3-E1 cells. Adding noggin, a BMP specific-antagonist, inhibited the acerogenin A-induced increase in the Osteocalcin, Osterix and Runx2 mRNA expression levels. These results indicated that acerogenin A stimulates osteoblast differentiation through BMP action, which is mediated by Runx2-dependent and Runx2-independent pathways.
authors.affiliation: Section of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Kihara
authors.forename: Saki
authors.initials: S
authors.lastname: Ichikawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akihisa
authors.forename: Je Tae
authors.initials: JT
authors.lastname: Woo
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Michi
authors.forename: Teruo
authors.initials: T
authors.lastname: Amagasa
authors.forename: Akira
authors.initials: A
authors.lastname: Yamaguchi
mesh_terms: D031002:Acer / Q000737:chemistry*; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis*; D001861:Bone Regeneration / Q000187:drug effects*; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010647:Phenyl Ethers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000096:biosynthesis; D000099176:Noggin Protein; D000097566:Cyclophanes
chemical_list: D019485:Bone Morphogenetic Proteins; D002352:Carrier Proteins; D050659:Core Binding Factor Alpha 1 Subunit; D036381:Diarylheptanoids; D015675:Osteocalcin; D010647:Phenyl Ethers; D012333:RNA, Messenger; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors; D000099176:Noggin Protein; C479466:Sp7 protein, mouse; C557988:acerogenin A; D000097566:Cyclophanes