title: Species identification of intestinal microsporidia using immunofluorescence antibody assays.
abstract: Abstract. The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), mo lecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spores using the Weber modified trichrome (WMT) staining technique were examined using IFA-MAbs. Of the 100 specimens examined, the microsporidial spores identified by IFA-MAbs were Enterocytozoon Bieneusi 42 (75%) Encephalitozoon intestinalis 7 (12.5%) and mixed infections 7 (12.5%). The sensitivity and specificity of IFA-MAbs in detecting microsporidial spores were 98% and 86%, respectively. The agreement between the WMT staining technique and IFA-MAbs was statistically significant by Kappa statistics (K = 0.840; p < 0.001). E. bieneusi was the commonest Microsporidia species isolated from the studied population; the presence of microsporidial spores detected by IFA-MAbs should be confirmed by other methods.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: M Abdulsalam
authors.initials: MA
authors.lastname: Al-Mekhlafi
authors.forename: M S
authors.initials: MS
authors.lastname: Fatmah
authors.forename: N
authors.initials: N
authors.lastname: Anisah
authors.forename: M
authors.initials: M
authors.lastname: Azlin
authors.forename: Hesham M
authors.initials: HM
authors.lastname: Al-Mekhlafi
authors.forename: M
authors.initials: M
authors.lastname: Norhayati
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D016819:Encephalitozoon / Q000145:classification* / Q000302:isolation & purification; D021862:Enterocytozoon / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D008296:Malaysia; D046529:Microscopy, Electron, Transmission; D016881:Microsporidiosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013172:Spores, Fungal; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal