title: Analyzing autophagy in Magnaporthe oryzae.
abstract: Magnaporthe oryzae is an important plant pathogenic fungus that greatly threatens the world's food security. Both genome-wide and individual gene studies have shown that the pathogenicity of the fungus is severely dependent on the intracellular autophagy process during appressoria development. This protocol discusses a systematic methodology to discover and monitor autophagy-related (ATG) genes in M. oryzae.
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Fei-Long
authors.initials: FL
authors.lastname: Zhuang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Lin
mesh_terms: D001343:Autophagy / Q000235:genetics / Q000502:physiology*; D058948:Cell Tracking / Q000379:methods; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics; D020082:Magnaporthe / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D016296:Mutagenesis / Q000502:physiology; D030781:Organisms, Genetically Modified; D012275:Oryza / Q000382:microbiology; D013045:Species Specificity; D033921:Vesicular Transport Proteins / Q000235:genetics
chemical_list: D033921:Vesicular Transport Proteins; D049452:Green Fluorescent Proteins