title: Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring.
abstract: A thermophilic bacterium, designated strain RHA1(T), was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China, and was characterized by using a polyphasic approach. Based on its phenotypic and phylogenetic characteristics, strain RHA1(T) was affiliated to the genus Laceyella. The strain formed white aerial and yellow-white substrate mycelia, bearing single endospores on short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major fatty acids were iso-C(15:0) (62.39%) and anteiso-C(15:0) (17.55%)(.) The predominant menaquinone was MK-9. The G+C content of the genomic DNA of strain RHA1(T) was 47.9 mol%. Based on DNA-DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain RHA1(T) is considered to represent a novel species of the genus Laceyella, for which the name Laceyella sediminis sp. nov. is proposed; the type strain is RHA1(T) (=DSM 45263(T)=CCTCC AA 208058(T)).
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.|Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming 650093, PR China.
authors.forename: Jiu-Jiu
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming 650093, PR China.
authors.forename: Lian-Bing
authors.initials: LB
authors.lastname: Lin
authors.affiliation: Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming 650093, PR China.
authors.forename: Lan-Lan
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming 650093, PR China.
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.affiliation: Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming 650093, PR China.
authors.forename: Yun-Lin
authors.initials: YL
authors.lastname: Wei
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012266:Ribose / Q000032:analysis; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D012266:Ribose; D005947:Glucose