title: BAG3 (BCL2-associated athanogene 3) interacts with MMP-2 to positively regulate invasion by ovarian carcinoma cells.
abstract: BAG3 (BCL2-associated athanogene 3) is a member of the BAG family proteins, which interact with and regulate Hsp70. Our aim in the present study was to correlate BAG3 expression in ovarian cancer with invasion and progression-free survival. We found that BAG3 interacts with MMP2 in cultured ovarian cancer cells, and that knockdown of BAG3 expression downregulated MMP2 expression and diminished cell motility and invasiveness. The incidence of BAG3 positivity was significantly higher at advanced clinical stages of ovarian cancer than at early stages. We suggest that BAG3 binds to MMP2 to positively regulate the process of cell invasion.
authors.affiliation: Department of Obstetrics and Gynecology, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Suzuki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Asuka
authors.initials: A
authors.lastname: Sugio
authors.forename: Akinori
authors.initials: A
authors.lastname: Hishiya
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Tanaka
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Endo
authors.forename: Shinichi
authors.initials: S
authors.lastname: Takayama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Saito
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D000077216:Carcinoma, Ovarian Epithelial; D002352:Carrier Proteins; D045744:Cell Line, Tumor; D002465:Cell Movement; D018572:Disease-Free Survival; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D002352:Carrier Proteins; D020778:Matrix Metalloproteinase 2