title: [Effect of benzo(a)pyrene on platelet aggregation and expression of P-selectin].
abstract: To investigate the effect of benzo(a)pyrene (BaP) on platelet aggregation and expression of P-selectin.
  
  
  
  Blood samples were collected from healthy volunteers and the platelets was washed. Platelet aggregation was monitored by aggregometer and the expression of P-seletin was detected by whole blood flow cytometry.
  
  
  
  BaP (10 μmol/L, 1 μmol/L and 0.1 μmol/L) did not induce platelet aggregation; however, preincubation with BaP (10 μmol/L) significantly enhanced ADP-induced platelet aggregation (P < 0.01) and platelet aggregation was (80 ± 10)%, while BaP-preincubation failed to enhance platelet aggregation under collagen and thrombin stimulation. Flow cytometry showed that preincubation with BaP increased ADP-induced, but not thrombin-induced P-selectin expression (P < 0.01).
  
  
  
  BaP can stimulate ADP-induced platelet aggregation and P-selectin expression, probably through the interaction with ADP-mediated signal pathway.
authors.affiliation: Zhejiang California International Nanosystems Institute, Hangzhou 310029, China. mumu_taq@163.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Tang
authors.forename: Yi-hua
authors.initials: YH
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology; D006801:Humans; D019007:P-Selectin / Q000097:blood / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology
chemical_list: D019007:P-Selectin; D001564:Benzo(a)pyrene; D000244:Adenosine Diphosphate; D003094:Collagen; D013917:Thrombin