title: A novel human polyomavirus closely related to the african green monkey-derived lymphotropic polyomavirus.
abstract: We identified a novel human polyomavirus from a kidney transplant patient under immunosuppressive treatment, by use of a generic PCR. The genome of the virus was completely amplified and sequenced. In phylogenetic analyses, it appeared as the closest relative to the African green monkey-derived lymphotropic polyomavirus (LPV). Further investigation of clinical samples from immunocompromised patients with specific nested PCR revealed additional positive samples, indicating that the virus naturally infects humans. The virus was tentatively named human polyomavirus 9 (HPyV9). The previously observed seroreactivity to LPV in human populations might find a partial explanation in the circulation of HPyV9.
authors.affiliation: Division of Viral Infections, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany.
authors.forename: Nelly
authors.initials: N
authors.lastname: Scuda
authors.forename: Jörg
authors.initials: J
authors.lastname: Hofmann
authors.forename: Sébastien
authors.initials: S
authors.lastname: Calvignac-Spencer
authors.forename: Klemens
authors.initials: K
authors.lastname: Ruprecht
authors.forename: Peter
authors.initials: P
authors.lastname: Liman
authors.forename: Joachim
authors.initials: J
authors.lastname: Kühn
authors.forename: Hartmut
authors.initials: H
authors.lastname: Hengel
authors.forename: Bernhard
authors.initials: B
authors.lastname: Ehlers
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops / Q000821:virology; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D016867:Immunocompromised Host; D007668:Kidney / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014180:Transplantation; D014412:Tumor Virus Infections / Q000821:virology*
chemical_list: D004279:DNA, Viral