title: Fat extraction from acid- and base-hydrolyzed food samples using accelerated solvent extraction.
abstract: This paper describes a new in-cell method for pursuing accelerated solvent extraction (ASE) prior to lipid analysis from food samples. It is difficult to pursue direct ASE with acid- or base-hydrolyzed samples due to the corrosive nature of the reagents and material limitations. In this study ion exchange based materials were used to remove acid or base reagents in-cell without compromising the recovery of lipids. The performance data are presented here for the new methods for lipid extraction for a variety of food samples and compared to the Mojonnier method. NIST Standard Reference Materials (SRM-1546 and SRM-1849) were used to validate the ASE methods. Excellent fat recoveries were obtained for the ASE methods. The new methods presented here enhance the utility of ASE and eliminate labor intensive protocols.
authors.affiliation: Dionex Corporation, 445 Lakeside Drive, Sunnyvale, California 94085, United States. smrahmat.ullah@dionex.com
authors.forename: S M Rahmat
authors.initials: SM
authors.lastname: Ullah
authors.forename: Brett
authors.initials: B
authors.lastname: Murphy
authors.forename: Brian
authors.initials: B
authors.lastname: Dorich
authors.forename: Bruce
authors.initials: B
authors.lastname: Richter
authors.forename: Kannan
authors.initials: K
authors.lastname: Srinivasan
mesh_terms: D000143:Acids / Q000737:chemistry; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D005223:Fats / Q000032:analysis / Q000302:isolation & purification*; D005504:Food Analysis; D006868:Hydrolysis; D012997:Solvents / Q000737:chemistry
chemical_list: D000143:Acids; D005223:Fats; D012997:Solvents