title: The selective action of D2 dopamine receptor antisense oligodeoxynucleotide on the expression of the dopamine receptor subtype mRNA in rat striatum.
abstract: We have evaluated the effect of the D₂ dopamine receptor antisense oligodeoxynucleotide (D₂ AS ODN) on the gene expression of all five dopamine receptor subtypes including D₁, D₂, D₃, D₄ and D₅ dopamine receptor in the rat striatum. The levels of D₂ dopamine receptor mRNA are significantly decreased at 6, 12, 24 h after the last injection of three time injections of D₂ AS ODN, although D₁, D₃, D₄ and D₅ subtype mRNA levels did not significantly reduced at any time. The present study is the first to demonstrate the selective effect of D₂ AS ODN on D₂ dopamine receptor mRNA among all five dopamine receptor subtypes and the effectiveness of D₂ AS ODN without 6-hydroxydopamine.
authors.affiliation: Department of Psychiatry and Behavioral Science, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Andoh
authors.forename: Masanobu
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Kosuke
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Oka
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017447:Receptors, Dopamine D1 / Q000096:biosynthesis* / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2