title: [Effect of notch signaling on differentiation of rat marrow mesenchymal stem cells into neurons induced by fasudil hydrochloride].
abstract: OBJECTIVE
  To investigate the effect of notch signaling on differentiation of rat bone marrow mesenchymal stem cells (MSCs) into neurons induced by fasudil hydrochloride.
  
  
  METHODS
  The experiments were divided into non-transfected group, transfected group (transfected with Rn-Notch1-siRNA), positive control group (transfected with Rn-MAPK-1 Control siRNA) and negative control group (transfected with negative control siRNA). Fasudil hydrochloride induced MSCs differentiating into neurons. The fluorescence expressed by transfected MSCs were observed under inverted fluorescence microscope. The expression of notch1 mRNA, Hes1 mRNA and MAPK1 mRNA in MSCs was detected by RT-PCR. The expression of Notch1 protein, NSE, neurofilament M (NF-M) and glial fibrillary acidic protein(GFAP)was detected by immunocytochemical method. The viability of MSCs was detected by MTT.
  
  
  RESULTS
  (1) The fluorescence of MSCs was mostly displayed after transfection for 72 h and the efficiency of transfection was up to 91.3% +/- 4.2%. Meanwhile, the notch1 mRNA and Hes1 mRNA expressed by MSCs of transfected group were significantly decreased (P < 0.05) and MTT displayed that the viability of MSCs was also significantly reduced (P < 0.05). (2) Fasudil hydrochloride could induce MSCs differentiate into neurons and the best efficiency of induction was observed in the transfected group. There was higher expression of NSE and neurofilament-M (NF-M) than the other groups (P < 0.05).
  
  
  CONCLUSIONS
  There may be notch1 signaling and Rho/Rho GTPase signaling synergy on differentiation of rat bone marrow stromal cell into neurons induced by fasudil hydrochloride and they jointly promote the differentiation of MSCs into neurons.
authors.affiliation: Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, China.
authors.forename: Er-Yi
authors.initials: EY
authors.lastname: Zhao
authors.forename: Liu-Dong
authors.initials: LD
authors.lastname: Wang
authors.forename: Quan-Qing
authors.initials: QQ
authors.lastname: Wen
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Guan
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Jing
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Wen
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Jia
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D051881:Receptor, Notch1 / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D051881:Receptor, Notch1; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C049347:fasudil