title: In vitro antiproliferative and antifungal activity of essential oils from Erigeron acris L. and Erigeron annuus (L.) Pers.
abstract: Antiproliferative and antifungal activities of essential oils from Erigeron acris root and herb and from Erigeron annuus herb were investigated. The cell viability assay was performed in cultured fibroblasts, cancer cell lines (MCF-7 and MDA-MBA-231), and endometrial adenocarcinoma (Ishikawa) cells as well as colon adenocarcinoma (DLD-1) cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The essential oil from E. acris root showed the highest antiproliferative activity in the MCF-7 cell line with an IC50 value of 14.5 microg/mL. No effect of the essential oil on normal cells at that concentration was found. Antifungal activity against various strains of five Candida species, i.e. C. albicans, C. glabrata, C. tropicalis, C. krusei, and C. parapsilosis, was tested by the microdilution method. It was found that all examined oils can be useful as antifungal agents against the above-mentioned species, but the essential oil of E. acris herb was the most active. Their minimum inhibitory concentrations (MIC) ranged from 30 to 0.4 microL/mL. The data presented suggest that essential oils from E. acris and E. annuus possess antifungal activity against Candida spp. and antiproliferative activity against breast cancer MCF-7 cells.
authors.affiliation: Department of Pharmacognosy, Medical University of Białystok, Mickiewicza 2a Str., 15-089 Białystok, Poland. jolanta.nazaruk@umwb.edu.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Nazaruk
authors.forename: Ewa
authors.initials: E
authors.lastname: Karna
authors.forename: Piotr
authors.initials: P
authors.lastname: Wieczorek
authors.forename: Paweł
authors.initials: P
authors.lastname: Sacha
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Tryniszewska
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D049919:Erigeron / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D009822:Oils, Volatile