title: Different B cell populations mediate early and late memory during an endogenous immune response.
abstract: Memory B cells formed in response to microbial antigens provide immunity to later infections; however, the inability to detect rare endogenous antigen-specific cells limits current understanding of this process. Using an antigen-based technique to enrich these cells, we found that immunization with a model protein generated B memory cells that expressed isotype-switched immunoglobulins (swIg) or retained IgM. The more numerous IgM(+) cells were longer lived than the swIg(+) cells. However, swIg(+) memory cells dominated the secondary response because of the capacity to become activated in the presence of neutralizing serum immunoglobulin. Thus, we propose that memory relies on swIg(+) cells until they disappear and serum immunoglobulin falls to a low level, in which case memory resides with durable IgM(+) reserves.
authors.affiliation: Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Pape
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Taylor
authors.forename: Robert W
authors.initials: RW
authors.lastname: Maul
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Gearhart
authors.forename: Marc K
authors.initials: MK
authors.lastname: Jenkins
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002470:Cell Survival; D005260:Female; D018858:Germinal Center / Q000166:cytology / Q000276:immunology; D007114:Immunization; D017578:Immunoglobulin Class Switching; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology*; D007156:Immunologic Memory; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D010798:Phycocyanin / Q000276:immunology; D010799:Phycoerythrin / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000941:Antigens; D007075:Immunoglobulin M; C002497:allophycocyanin; D010798:Phycocyanin; D010799:Phycoerythrin; D051997:ADP-ribosyl Cyclase 1