title: Acidic buffer or plus cyclosporine a postconditioning protects isolated rat hearts against ischemia-reperfusion injury.
abstract: BACKGROUND: It is well documented that transient acidosis during reperfusion is protective. The aim of this study was to evaluate the cardioprotection of acidic buffer or plus cyclosporine A in isolated rat hearts after cardioplegic arrest. METHODS: Langendorff-perfused Sprague-Dawley rat hearts were perfused for 20 min with Krebs-Henseleit (K-H) buffer followed by 30 min of crystalloid cardioplegia and 60 min of reperfusion. Control hearts were perfused with Krebs-Henseleit buffer. Acidic buffer post-conditioning hearts were perfused with acidic K-H buffer (pH 6.8) for the first 3 min of reperfusion. Acidic buffer plus cyclosporine A hearts were perfused with K-H acidic buffer (pH 6.8) containing cyclosporine A (0.2 μmol/L) for the first 3 min of reperfusion. RESULTS: Compared with the control group, acidic buffer or plus cyclosporine A post-conditioning significantly improved myocardial performance, decreased cytochrome C release into the cytosol, increased Bcl-2 expression and decreased Bax expression, decreased sensitivity of mPTP-opening to [Ca2+] and the rate of apoptosis after reperfusion. CONCLUSION: These findings suggested that acidic buffer or plus cyclosporine A post-conditioning prevented apoptosis-related mitochondrial permeabilization and provided the myocardial protection after cardioplegic arrest.
authors.affiliation: Fuwai Hospital and Cardiovascular Institute, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, Peoples Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Duan
authors.forename: Bingyang
authors.initials: B
authors.lastname: Ji
authors.forename: Kun
authors.initials: K
authors.lastname: Yu
authors.forename: Jinping
authors.initials: J
authors.lastname: Liu
authors.forename: Feilong
authors.initials: F
authors.lastname: Hei
authors.forename: Cun
authors.initials: C
authors.lastname: Long