title: Synthesis of poly(propylene glycol)-block-polyethylenimine triblock copolymers for the delivery of nucleic acids.
abstract: LPEIs, which are efficient DNA transfection agents, were found to be far less effective for the delivery of siRNAs. Here, two amphiphilic triblock copolymers LPEI(50) -b-PPG(36) -b-LPEI(50) (2) and LPEI(14) -b-PPG(68) -b-LPEI(14) (4) have been synthesized. The transfection assays showed that compound 2 was efficient for DNA transfection whilst it was almost inactive for siRNA delivery. In contrast, polymer 4 was inefficient for DNA transfection while it showed capabilities for siRNA delivery. Taken together, our results indicate that the properties required for DNA and siRNA delivery are different. Moreover, we show that introduction of a hydrophobic segment that allows self-assembly confers siRNA delivery capacities.
authors.affiliation: Institut Chimie et Matériaux de Paris Est, UMR 7182 Equipe Systèmes Polymères Complexes, Thiais, France.
authors.forename: Blandine
authors.initials: B
authors.lastname: Brissault
authors.forename: Christian
authors.initials: C
authors.lastname: Leborgne
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
authors.forename: Christine
authors.initials: C
authors.lastname: Guis
authors.forename: Antoine
authors.initials: A
authors.lastname: Kichler
mesh_terms: D002460:Cell Line; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D010316:Particle Size; D010957:Plasmids; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D011108:Polymers / Q000138:chemical synthesis*; D011409:Propylene Glycols / Q000138:chemical synthesis*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry; D014162:Transfection / Q000379:methods*
chemical_list: D011108:Polymers; D011409:Propylene Glycols; D034741:RNA, Small Interfering; D013501:Surface-Active Agents; C012504:polypropylene glycol; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases