title: Construction, screening and evaluation of a derivative recombinant adenovirus for the optimal siRNA targeting of a novel gene (HA117).
abstract: At this study, we screened for an optimal siRNA to target a novel gene named HA117 and constructed and evaluated a recombinant adenovirus carrying the DNA template for the transcription of the optimal HA117 siRNA to examine the function of HA117 and its possible mechanism of action in CW-2 cell lines. An HAi5-carrying recombinant adenovirus (Ad-HAi5) was successfully constructed and evaluated. The results show that, among five pairs of DNA templates, siRNA transcribed from HAi5 gave the strongest interference with the novel gene HA117. The expression of the exogenous HA117 gene increased drug resistance in CW-2 cells. We hypothesized that this action may be a result of HA117 through inhibiting apoptosis in CW-2 cells.
authors.affiliation: The Infection and Digestology Department of Affiliated Children's Hospital of Chongqing Medical University, Key Laboratory of Developmental Diseases in Childhood of Chongqing Medical University and Education Ministry, PR China. gaihuan168@163.com
authors.forename: Gaihuan
authors.initials: G
authors.lastname: Zheng
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Guo
authors.forename: Xianqing
authors.initials: X
authors.lastname: Jin
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Su
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple / Q000235:genetics*; D018390:Gene Targeting; D018922:HL-60 Cells; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D011995:Recombination, Genetic; D013698:Templates, Genetic
chemical_list: D034741:RNA, Small Interfering