title: Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin.
abstract: The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV-visible absorption spectroscopy. A value of 1.3×10(7) M(-1) was obtained for the antibody-antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.
authors.affiliation: School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Paul
authors.forename: Deepen
authors.initials: D
authors.lastname: Paul
authors.forename: George R
authors.initials: GR
authors.lastname: Fern
authors.forename: Asim K
authors.initials: AK
authors.lastname: Ray
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D002417:Cattle; D006041:Goats; D007074:Immunoglobulin G / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011817:Rabbits; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D007074:Immunoglobulin G; D012834:Silver