title: [Molecular cloning of genes differentially expressed in human lung squamous cell carcinoma using suppression subtractive hybridization].
abstract: BACKGROUND
  To clone and identify genes differentially expressed in human lung squamous cell carcinoma (LSCC).
  
  
  METHODS
  A subtracted cDNA library of human LSCC was constructed by suppression subtracted hybridization (SSH) method. After screening, the subtracted library clones representing mRNAs that were truly differentially expressed in LSCC but not in its adjacent non cancerous tissues were selected to identify by RT-PCR and DNA sequencing were performed. Nucleic acid homology searches were performed using the BLAST program.
  
  
  RESULTS
  By this technique, 10 differentially expressed gene cDNA fragments of LSSC were obtained. Two were novel and eight were already known genes.
  
  
  CONCLUSIONS
  SSH is a useful technique with high sensitivity for the detection of differential genes expression in LSCC and an effective method to clone novel genes.
authors.affiliation: Department of Thoracic Surgery, The People's Hospital, Peking University, Beijing 100044, P.R.China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Danlei
authors.initials: D
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Guanchao
authors.initials: G
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang