title: Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay.
abstract: High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples.
authors.affiliation: BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, 12489, Berlin, Germany.
authors.forename: Arnold
authors.initials: A
authors.lastname: Bahlmann
authors.forename: Jana
authors.initials: J
authors.lastname: Falkenhagen
authors.forename: Michael G
authors.initials: MG
authors.lastname: Weller
authors.forename: Ulrich
authors.initials: U
authors.lastname: Panne
authors.forename: Rudolf J
authors.initials: RJ
authors.lastname: Schneider
mesh_terms: D002220:Carbamazepine / Q000737:chemistry*; D017332:Cetirizine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D003432:Cross-Linking Reagents; D014874:Water Pollutants, Chemical; D002220:Carbamazepine; D017332:Cetirizine