title: Immunosuppressive activity of florfenicol on the immune responses in mice.
abstract: Florfenicol is a new type of broad-spectrum antibacterial that has been used in veterinary clinics. It shows immunosuppressive activity on the immune responses to ovalbumin (OVA) in mice. In the present study, florfenicol suppressed lipopolysaccharide (LPS)-stimulated splenocyte proliferation in a concentration-dependent manner in vitro and in vivo. BALB/c mice were immunized subcutaneously with OVA on days 1 and 4. Following the second immunization, mice were treated with a single daily oral dose of florfenicol (50, 100, and 200 mg/kg) for 10 consecutive days. On day 14, blood samples were collected to analyze OVA-specific IgG, IgG1, and IgG2b antibodies, and splenocytes were harvested to assess lymphocyte proliferation, CD3(+) T and CD19(+) B lymphocyte subsets. The results presented here demonstrate that florfenicol not only significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation but also decreased the percentage of CD19(+) B cells in a dose-dependent manner and suppressed CD3(+) T cell at high doses. Moreover, OVA-specific IgG, IgG1 and IgG2b titers in OVA-immunized mice were reduced by florfenicol. These results suggest that florfenicol could suppress humoral and cellular immune responses in mice.
authors.affiliation: Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 30062, People's Republic of China.
authors.forename: Guan
authors.initials: G
authors.lastname: Shuang
authors.forename: Song
authors.initials: S
authors.lastname: Yu
authors.forename: Guo
authors.initials: G
authors.lastname: Weixiao
authors.forename: Wang
authors.initials: W
authors.lastname: Dacheng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhichao
authors.forename: Lu
authors.initials: L
authors.lastname: Jing
authors.forename: Deng
authors.initials: D
authors.lastname: Xuming
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000906:Antibodies / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D007165:Immunosuppression Therapy; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology; D013839:Thiamphenicol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D010047:Ovalbumin; C035534:florfenicol; D013839:Thiamphenicol