title: [Effect of mesenteric lymph duct ligation on erythrocyte rheology in acute hemorrhagic rats].
abstract: OBJECTIVE
  To observe the effects of mesenteric lymph duct (MLD) ligation on erythrocyte rheology in acute hemorrhagic rats.
  
  
  METHODS
  Twenty male Wistar rats were randomly divided into hemorrhage group and ligation group (n = 10). Blood (one fourth of body whole blood volume) was withdrawn through right common carotid arteries after rats were anesthetized. In ligation group, the MLD was ligated after hemorrhage, and only threading under the MLD in hemorrhage group. The survival situation at 24 h was recorded. After 24 h, survival rats were anesthetized again, blood sample was withdrawn through left common carotid artery rapidly. And the erythrocyte sedimentation rate (ESR), electrophoresis of erythrocytes, hematocrit (Hct) were determined in blood samples of before and after hemorrhage, the erythrocytes aggregation and deformability indices were calculated.
  
  
  RESULTS
  It showed that the ligation group survival (9 rats alive) was slightly better than that in hemorrhage group (6 rats alive). The results of erythrocyte rheology indices showed that the ESR, K value of equation, K value of emendation and electrophoresis time in hemorrhage group and ligation group were higher or longer than those before hemorrhage, the erythrocyte deformability was reduced significantly, respectively. And the erythrocytes aggregation index in hemorrhage group was increased, the electrophoresis length and migration of erythrocyte in hemorrhage group were lower than those before hemorrhage, respectively. But compared with hemorrhage group, the ESR, K value of equation, K value of emendation, erythrocytes aggregation index and electrophoresis time in ligation group were lower, the electrophoresis lenght, migration and deformability of erythrocyte were increased significantly.
  
  
  CONCLUSIONS
  The results indicate that the higher erythrocyte aggregation ability, lower electrophoresis function and deformability are caused by acute hemorrhage in rats, and the MLD ligation can improve the abnormal erythrocyte rheology.
authors.affiliation: Institute of Microcirculation, Hebei North College, Zhangjiakou 075000, China.
authors.forename: Zi-Gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yong-Quan
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Niu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000473:pathology*; D006470:Hemorrhage / Q000601:surgery*; D008026:Ligation; D042601:Lymphatic Vessels / Q000601:surgery*; D008297:Male; D008643:Mesentery / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology; D012771:Shock, Hemorrhagic / Q000601:surgery*