title: Effect of cinnamoyl and flavonol glucosides derived from cherry blossom flowers on the production of advanced glycation end products (AGEs) and AGE-induced fibroblast apoptosis.
abstract: Cherry blossom flowers are familiar to the Japanese, and some species of the flowers soaked in salty vinegar are used as processed foods. The constituents of aqueous ethanol extract from cherry blossom (Prunus lannesiana) flowers (CBE) were examined and cinnamoyl and flavonol glucosides were isolated. To elucidate the pharmacological functions of CBE and its constituents, their effects on the production of advanced glycation end products (AGEs) and on AGE-induced fibroblast damage were examined. CBE and 1-O-(E)-caffeoyl-β-D-glucopyranoside (CaG), a principal compound in CBE, significantly suppressed the production of AGEs derived from glucose and albumin at 100 μg/mL. Among the flavonol glucosides, quercetin 3-O-β-D-glucopyranoside (QG) exhibited potent suppressive activity (IC50 : 30 μg/mL). CBE and CaG suppressed glyoxal-induced AGE production in fibroblasts at 10 μg/mL, but QG did not. In addition, CBE and CaG recovered collagen lattice formation consisting of collagen and glycated fibroblasts at 10 μg/mL. Moreover, CBE and its constituents, except kaempferol 3-O-(6″-malony)-β-D-glucopyranoside, significantly suppressed fibroblast apoptosis induced by carboxymethyl lysine-collagen at 10 μg/mL. These results show that cinnamoyl and flavonol glucosides of cherry blossom flowers suppress AGE production and AGE-induced fibroblast apoptosis. Cherry blossom flowers may be effective against skin AGE production and fibroblast damage by AGEs.
authors.affiliation: Research and Development Division, Oryza Oil and Fat Chemical Co. Ltd, 1 Numata, Kitagata-cho, Ichinomiya, Aichi, 493-8001, Japan. kaihatsu@mri.biglobe.ne.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Seiko
authors.initials: S
authors.lastname: Nakamura
authors.forename: Makoto
authors.initials: M
authors.lastname: Morioka
authors.forename: Junji
authors.initials: J
authors.lastname: Tanaka
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002109:Caffeic Acids / Q000494:pharmacology; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects*; D044948:Flavonols / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C587128:1,4-di-O-(E)-caffeoyl-beta-D-glucopyranoside; D002109:Caffeic Acids; D044948:Flavonols; D005960:Glucosides; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D003094:Collagen; D011794:Quercetin; C110309:quercetin 3-O-glucopyranoside