title: Parvovirus 4 in French in-patients: a study of hemodialysis and lung transplant cohorts.
abstract: The epidemiology and the clinical implication of human parvovirus 4 (PARV4) in human populations is still under evaluation. The distribution of PARV4 DNA was determined in cohorts of French hemodialysis and lung transplant patients. Plasma samples (n=289) were tested for PARV4 by real-time PCR assay (ORF2), and amplification products selected at random were sequenced. Analysis of available serological and biological markers was also undertaken. Fifty-seven samples out of 185 (30.8%) were positive for PARV4 DNA in the cohort of hemodialysis patients. A higher prevalence of the virus was identified in patients with markers of HBV infection. PARV4 was also identified in 14 out of 104 samples (13.5%) from lung transplant recipients, with no clear-cut association with available clinical markers. Point mutations located on the zone of real-time detection were identified for some amplification products. This study describes the detection of PARV4 in the blood of hemodialysis and lung transplanted patients with significant difference in prevalence in these two cohorts. Further studies will be needed in order to understand better both the potential implication in host health and the natural history of this virus.
authors.affiliation: Viral Emergence and Co-evolution Unit, French Blood Agency Alpes-Mediterranee and University of Mediterranee, Marseille, France.
authors.forename: Mhammed
authors.initials: M
authors.lastname: Touinssi
authors.forename: Martine
authors.initials: M
authors.lastname: Reynaud-Gaubert
authors.forename: Carine
authors.initials: C
authors.lastname: Gomez
authors.forename: Pascal
authors.initials: P
authors.lastname: Thomas
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dussol
authors.forename: Yvon
authors.initials: Y
authors.lastname: Berland
authors.forename: Agnes
authors.initials: A
authors.lastname: Basire
authors.forename: Christophe
authors.initials: C
authors.lastname: Picard
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cantaloube
authors.forename: Philippe
authors.initials: P
authors.lastname: de Micco
authors.forename: Philippe
authors.initials: P
authors.lastname: Biagini
mesh_terms: D015331:Cohort Studies; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology; D017992:Parvovirus / Q000145:classification / Q000302:isolation & purification*; D010949:Plasma / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D006435:Renal Dialysis / Q000009:adverse effects*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral