title: Sensitive electrochemical detection of superoxide anion using gold nanoparticles distributed poly(methyl methacrylate)-polyaniline core-shell electrospun composite electrode.
abstract: In the present communication, a novel composite nanofibrous electrode is developed for the detection of superoxide anion (O(2)˙(-)) in phosphate buffered saline (PBS). The composite fiber electrode is fabricated by dispersing gold nanoparticles onto poly(methyl methacrylate) (PMMA)-polyaniline (PANI) core-shell electrospun nanofibers. The constructed architecture is proven to be a favorable environment for the immobilization of the enzyme, superoxide dismutase (SOD). Direct electron transfer is achieved between SOD and the electrode with an electron transfer rate constant of 8.93 s(-1). At an applied potential of +300 mV, PMMA/PANI-Au(nano)/SOD-ESCFM shows highly sensitive detection of O(2)˙(-). In addition to this, quantification of different activities of SOD is realized at PMMA/PANI-Au(nano)/SOD-ESCFM. These analytical features offer great potential for construction of the third-generation O(2)˙(-) biosensor.
authors.affiliation: Department of Chemistry Graduate School, Kyungpook National University, Daegu, 702-701, South Korea.
authors.forename: Padmanabhan
authors.initials: P
authors.lastname: Santhosh
authors.forename: Kalayil Manian
authors.initials: KM
authors.lastname: Manesh
authors.forename: Se-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Sivaperumal
authors.initials: S
authors.lastname: Uthayakumar
authors.forename: Anantha Iyengar
authors.initials: AI
authors.lastname: Gopalan
authors.forename: Kwang-Pill
authors.initials: KP
authors.lastname: Lee
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism; D013481:Superoxides / Q000032:analysis*
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; D013481:Superoxides; D006046:Gold; D019904:Polymethyl Methacrylate; D013482:Superoxide Dismutase