title: Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua.
abstract: The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.
authors.affiliation: Nofima Norconserv AS, Stavanger, Norway. trond.lovdal@nofima.no
authors.forename: Trond
authors.initials: T
authors.lastname: Løvdal
authors.forename: Maria Befring
authors.initials: MB
authors.lastname: Hovda
authors.forename: Benny
authors.initials: B
authors.lastname: Björkblom
authors.forename: Simon G
authors.initials: SG
authors.lastname: Møller
mesh_terms: D001386:Azides / Q000737:chemistry; D015169:Colony Count, Microbial; D006358:Hot Temperature; D008087:Listeria / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D050296:Microbial Viability; D016133:Polymerase Chain Reaction / Q000379:methods*; D011419:Propidium / Q000031:analogs & derivatives / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001386:Azides; C533957:propidium monoazide; D011419:Propidium