title: The tungsten metallome of Pyrococcus furiosus.
abstract: The tungsten metallome of the hyperthermophilic archaeon Pyrococcus furiosus has been investigated using electroanalytical metal analysis and native-native 2D-PAGE with the radioactive tungsten isotope (187)W (t(1/2) = 23.9 h). P. furiosus cells have an intracellular tungsten concentration of 29 μM, of which ca. 30% appears to be free tungsten, probably in the form of tungstate or polytungstates. The remaining 70% is bound by five different tungsten enzymes: formaldehyde ferredoxin oxidoreductase, aldehyde ferredoxin oxidoreductase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase and the tungsten-containing oxidoreductases WOR4 and WOR5. The membrane proteome of P. furiosus is devoid of tungsten. The differential expression, as measured by the tungsten level, of the five soluble tungsten enzymes when the cells are subjected to a cold-shock shows a strong correlation with previously published DNA microarray analyses.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Sevcenco
authors.forename: Martijn W H
authors.initials: MW
authors.lastname: Pinkse
authors.forename: Emile
authors.initials: E
authors.lastname: Bol
authors.forename: Gerard C
authors.initials: GC
authors.lastname: Krijger
authors.forename: Hubert Th
authors.initials: HT
authors.lastname: Wolterbeek
authors.forename: Peter D E M
authors.initials: PD
authors.lastname: Verhaert
authors.forename: Peter-Leon
authors.initials: PL
authors.lastname: Hagedoorn
authors.forename: Wilfred R
authors.initials: WR
authors.lastname: Hagen
mesh_terms: D019843:Archaeal Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D007525:Isoelectric Focusing; D008667:Metalloproteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics; D020027:Pyrococcus furiosus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D012995:Solubility; D014414:Tungsten / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D008667:Metalloproteins; D014414:Tungsten