title: Flower: extracting information from pyrosequencing data.
abstract: The SFF file format produced by Roche's 454 sequencing technology is a compact, binary format that contains the flow values that are used for base and quality calling of the reads. Applications, e.g. in metagenomics, often depend on accurate sequence information, and access to flow values is important to estimate the probability of errors. Unfortunately, the programs supplied by Roche for accessing this information are not publicly available. Flower is a program that can extract the information contained in SFF files, and convert it to various textual output formats.
  
  
  BACKGROUND
  Flower is freely available under the General Public License.
authors.affiliation: The Norwegian Marine Data Centre, Institute of Marine Research, Bergen, Norway. ketil.malde@imr.no
authors.forename: Ketil
authors.initials: K
authors.lastname: Malde
mesh_terms: D001483:Base Sequence; D017422:Sequence Analysis, DNA; D012984:Software