title: From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family.
abstract: Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ~400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.
authors.affiliation: National Oceanography Centre, University of Southampton, Southampton SO143ZH, UK.
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiedenmann
authors.forename: Susan
authors.initials: S
authors.lastname: Gayda
authors.forename: Virgile
authors.initials: V
authors.lastname: Adam
authors.forename: Franz
authors.initials: F
authors.lastname: Oswald
authors.forename: Karin
authors.initials: K
authors.lastname: Nienhaus
authors.forename: Dominique
authors.initials: D
authors.lastname: Bourgeois
authors.forename: G Ulrich
authors.initials: GU
authors.lastname: Nienhaus
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001703:Biophysics / Q000379:methods; D004583:Electrons; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008027:Light; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D008956:Models, Chemical; D017354:Point Mutation; D011487:Protein Conformation; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D014981:Xenopus
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins