title: Decreased expression of matrix metalloproteinase-9 and increased expression of tissue inhibitors of matrix metalloproteinase-1 in paratuberculosis-infected cattle in the ELISA-negative subclinical stage.
abstract: We investigated the gene expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitors of matrix metalloproteinases-1 (TIMP-1) in peripheral blood cells from infected cattle with Mycobacterium avium subsp. paratuberculosis (Map) in the ELISA-negative subclinical stage compared with uninfected control cattle. Significant decreased MMP-9 expression and increased TIMP-1 expression were found in peripheral blood cells from Map-infected cattle after stimulation with Map lysate and Map purified protein derivative (PPD) than in control cattle by real-time RT-PCR analysis. In contrast to the uninfected controls, the activity of MMP-9 was also decreased in peripheral blood cell culture supernatants from Map-infected cattle at 24 hr after Map lysate and MapPPD stimulation by gelatin zymography analysis. As a result, the MMP-9 may play an important role in the development of Mycobacterium avium subsp. paratuberculosis disease.
authors.affiliation: Research Team for Paratuberculosis, National Institute of Animal Health, Tsukuba, Japan.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
authors.lastname: Aodon-geril
authors.forename: Yujing
authors.initials: Y
authors.lastname: Shu
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Momotani
authors.forename: Reiko
authors.initials: R
authors.lastname: Nagata
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Eiichi
authors.initials: E
authors.lastname: Momotani
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D015870:Gene Expression; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D016927:Mycobacterium avium subsp. paratuberculosis; D010283:Paratuberculosis / Q000235:genetics*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D020780:Matrix Metalloproteinase 9