title: Genetic analysis of Anisakis typica (Nematoda: Anisakidae) from cetaceans of the northeast coast of Brazil: new data on its definitive hosts.
abstract: Anisakids from 5 different species of cetacean, Kogia breviceps, Peponocephala electra, Stenella clymene, Stenella longirostris and Steno bredanensis, were submitted to genetic analysis. Adults and larvae fixed in ethanol-formalin-acetic acid or in 70% ethanol for periods ranging from 10 months to 10 years were isolated from 9 cetaceans stranded on Ceará coast, Northeast Brazil. The 18S rDNA gene, ITS1, and specific Anisakis typica ITS regions were amplified by PCR. 18S rDNA and ITS1 region confirmed Anisakis sp. morphological identification but also detected the presence of Aspergillus sp. in longer preserved samples. All samples were identified as A. typica by ITS species-specific PCR. The study report three new definitive hosts of A. typica from the Brazilian Atlantic coast by genetic analysis: P. electra, K. breviceps, and S. clymene.
authors.affiliation: Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, Brazil. alena@ioc.ficoruz.br
authors.forename: Alena M
authors.initials: AM
authors.lastname: Iñiguez
authors.forename: Vitor L
authors.initials: VL
authors.lastname: Carvalho
authors.forename: Monica R Alves
authors.initials: MR
authors.lastname: Motta
authors.forename: Diana C Sousa Nunes
authors.initials: DC
authors.lastname: Pinheiro
authors.forename: Ana C Paulo
authors.initials: AC
authors.lastname: Vicente
mesh_terms: D000818:Animals; D017129:Anisakiasis / Q000469:parasitology / Q000662:veterinary*; D017163:Anisakis / Q000033:anatomy & histology / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D001938:Brazil; D002591:Cetacea / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S