title: Impact of amino acid mutations in PB2, PB1-F2, and NS1 on the replication and pathogenicity of pandemic (H1N1) 2009 influenza viruses.
abstract: Here, we assessed the effects of PB1-F2 and NS1 mutations known to increase the pathogenicity of influenza viruses on the replication and pathogenicity in mice of pandemic (H1N1) 2009 influenza viruses. We also characterized viruses possessing a PB1-F2 mutation that was recently identified in pandemic (H1N1) 2009 influenza virus isolates, with and without simultaneous mutations in PB2 and NS1. Our results suggest that some NS1 mutations and the newly identified PB1-F2 mutation have the potential to increase the replication and/or pathogenicity of pandemic (H1N1) 2009 influenza viruses.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI 53711, USA.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ozawa
authors.forename: Sarmila
authors.initials: S
authors.lastname: Basnet
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Burley
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.forename: Masato
authors.initials: M
authors.lastname: Hatta
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000473:pathology / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication
chemical_list: C085820:INS1 protein, influenza virus; C442816:PB1-F2 protein, Influenza A virus; C502893:PB2 protein, influenza virus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins