title: IgE and IgG(4) autoantibodies against DFS70/LEDGF in atopic dermatitis.
abstract: BACKGROUND
  DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.
  
  
  OBJECTIVE
  Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG(4)-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.
  
  
  RESULTS
  Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG(4)-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.
  
  
  CONCLUSIONS
  We conclude that serum IgE-anti-DFS70 aAbs and IgG(4)-anti-DFS70 aAbs may be related to the severity of AD.
authors.affiliation: Division of Connective Tissue and Autoimmunity, Department of Dermatology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.
authors.forename: Kanako
authors.initials: K
authors.lastname: Watanabe
authors.forename: Yoshinao
authors.initials: Y
authors.lastname: Muro
authors.forename: Kazumitsu
authors.initials: K
authors.lastname: Sugiura
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tomita
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D018925:Chemokines / Q000097:blood; D003876:Dermatitis, Atopic / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D036341:Intercellular Signaling Peptides and Proteins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014157:Transcription Factors / Q000276:immunology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001323:Autoantibodies; D018925:Chemokines; D007074:Immunoglobulin G; D036341:Intercellular Signaling Peptides and Proteins; C504649:PSIP1 protein, human; D014157:Transcription Factors; D007073:Immunoglobulin E