title: Glucose tolerance and left ventricular pressure-volume relationships in frequently used mouse strains.
abstract: We investigated glucose tolerance and left ventricular contractile performance in 4 frequently used mouse strains (Swiss, C57BL/6J, DBA2, and BalbC) at 24 weeks. Glucose tolerance was tested by measuring blood glucose levels in time after intraperitoneal glucose injection (2 mg/g body weight). Left ventricular contractility was assessed by pressure-conductance analysis. Peak glucose levels and glucose area under the curve were higher (all P < .05) in C57BL/6J (418 ± 65 mg/dL and 813 ± 100 mg·h/dL) versus Swiss (237 ± 66 mg/dL and 470 ± 126 mg·h/dL), DBA2 (113 ± 20 mg/dL and 304 ± 49 mg·h/dL, P < .01), and BalbC mice (174 ± 55 mg/dL and 416 ± 70 mg·h/dL). Cardiac output was higher (all P < .05) in Swiss (14038 ± 4530 μL/min) versus C57BL/6J (10405 ± 2683 μL/min), DBA2 (10438 ± 3251 μL/min), and BalbC mice (8466 ± 3013 μL/min). Load-independent left ventricular contractility assessed as recruitable stroke work (PRSW) was comparable in all strains. In conclusion, glucose tolerance and load-dependent left ventricular performance parameters were different between 4 mice background strains, but PRSW was comparable.
authors.affiliation: Division of Experimental Cardiac Surgery, Department of Cardiovascular Diseases, K.U.Leuven, 3000 Leuven, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: Oosterlinck
authors.forename: Annelies
authors.initials: A
authors.lastname: Vanderper
authors.forename: Willem
authors.initials: W
authors.lastname: Flameng
authors.forename: Paul
authors.initials: P
authors.lastname: Herijgers
mesh_terms: D000818:Animals; D005951:Glucose Tolerance Test; D006339:Heart Rate / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D013318:Stroke Volume / Q000187:drug effects / Q000502:physiology*; D017725:Ventricular Pressure / Q000187:drug effects / Q000502:physiology*
chemical_list: D007545:Isoproterenol