title: A highly efficient ADH-coupled NADH-recycling system for the asymmetric bioreduction of carbon-carbon double bonds using enoate reductases.
abstract: The asymmetric bioreduction of activated alkenes catalyzed by flavin-dependent enoate reductases from the OYE-family represents a powerful method for the production of optically active compounds. For its preparative-scale application, efficient and economic NADH-recycling is crucial. A novel enzyme-coupled NADH-recycling system is proposed based on the concurrent oxidation of a sacrificial sec-alcohol catalyzed by an alcohol dehydrogenase (ADH-A). Due to the highly favorable position of the equilibrium of ene-reduction versus alcohol-oxidation, the cosubstrate is only required in slight excess.
authors.affiliation: Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Tauber
authors.forename: Melanie
authors.initials: M
authors.lastname: Hall
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
authors.forename: Walter M F
authors.initials: WM
authors.lastname: Fabian
authors.forename: Kurt
authors.initials: K
authors.lastname: Faber
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Glueck
mesh_terms: D019840:2-Propanol / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000475:Alkenes / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology*; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology*; D017355:Zymomonas / Q000201:enzymology*
chemical_list: D000475:Alkenes; D009243:NAD; D010088:Oxidoreductases; D000426:Alcohol Dehydrogenase; D019840:2-Propanol