title: [Chiral separation of fluvastatin enantiomers with in vitro cellular method].
abstract: To establish a chiral separation method for determination of fluvastatin enantiomer with in vitro cellular model.
  
  
  
  The determination was performed on Chiralpak AD column (4.6 mm × 250 mm); and the phase consisted of hexane-isopropanol-trifluoroacetic acid (90:10:0.1) at a flow rate of 0.5 ml/min with UV detection of 239 nm.
  
  
  
  The standard curve was linear over the concentration range of 20 μmol/L-300 μmol/L (r² = 0.9993, r² = 0.9997). The recovery for this assay was (99.4 ± 0.8)%, precision for inter-assay and intra-assay was <10 %.
  
  
  
  The normal-phase HPLC chiral separation method was accurate and suitable for study on the stereoselectivity of fluvastatin with in vitro cellular model.
authors.affiliation: Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Bo-xuan
authors.initials: BX
authors.lastname: Qu
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Lu-shan
authors.initials: LS
authors.lastname: Yu
authors.forename: Hui-di
authors.initials: HD
authors.lastname: Jiang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhou
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
mesh_terms: D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005229:Fatty Acids, Monounsaturated / Q000302:isolation & purification*; D000077340:Fluvastatin; D007211:Indoles / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D005229:Fatty Acids, Monounsaturated; D007211:Indoles; D000077340:Fluvastatin