title: Polymorphonuclear neutrophils from JAK2(V617F) positive MPD patients do not support hypercoagulability: A study with calibrated automated thrombography (CAT).
abstract: Essential thrombocythemia and polycythemia vera are myeloproliferative disorders (MPD) with an elevated thrombotic risk. Leukocytosis has recently emerged as a new risk factor and there is increasing evidence that polymorphonuclear neutrophils (PMN) are involved. Procoagulant activity (PCA) of PMN in MPD has not yet been investigated. PCA of PMN from 22 patients with JAK2(V617F) positive MPD and 26 healthy subjects was studied using calibrated automated thrombography: in vitro thrombin generation induced with 1 pM tissue factor in the presence of added procoagulant phospholipids. There were no differences between patients and controls regarding the ability of PMN to increase thrombin generation. More surprisingly, basal thrombin generation in acellular MPD-plasma was found decreased for as yet unknown reasons. The presence of an active protein C pathway or platelets might provide a better insight into the coagulation phenotype in MPD.
authors.affiliation: CHU Nancy, Service d'hématologie biologique, pôle laboratoires, Nancy, France. julien.perrin@chu-nancy.fr
authors.forename: Julien
authors.initials: J
authors.lastname: Perrin
authors.forename: Dana
authors.initials: D
authors.lastname: Ranta
authors.forename: Fabienne
authors.initials: F
authors.lastname: Empereur
authors.forename: Claude
authors.initials: C
authors.lastname: Vigneron
authors.forename: Pierre
authors.initials: P
authors.lastname: Feugier
authors.forename: Thomas
authors.initials: T
authors.lastname: Lecompte
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001780:Blood Coagulation Tests; D005260:Female; D006801:Humans; D053614:Janus Kinase 2 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000503:physiopathology*; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology*; D011087:Polycythemia Vera / Q000378:metabolism / Q000503:physiopathology; D013917:Thrombin / Q000378:metabolism; D013920:Thrombocythemia, Essential / Q000378:metabolism / Q000503:physiopathology; D019851:Thrombophilia / Q000378:metabolism*
chemical_list: D053614:Janus Kinase 2; D013917:Thrombin