title: [Effect of Danshensu on fibronectin and collagen-1 secretion induced by high glucose in human peritoneal mesothelial cells].
abstract: OBJECTIVE
  To investigate the possible role of Danshensu on fibronectin (FN) and collagen-I (Col-I) secretion induced by high glucose in human peritoneal mesothelial cells (HPMCs).
  
  
  METHODS
  HPMCs were treated with high glucose and Danshensu at different concentrations. The mRNA expression of FN, Col-I, endothelin-1 (ET-1), and heme oxygenase-1 (HO-1) were examined by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of FN, Col-I HO-1 and ET-1 were analyzed by enzyme-linked immunosorbent assay or immunofluorescence method.
  
  
  RESULTS
  The expression of protein and mRNA of FN and Col-I were attenuated by Danshensu in both dose-dependent and time-dependent manner. The mRNA and protein levels of ET-1 were decreased, and the mRNA and protein levels of HO-1 increased in the Danshensu groups in a dose-dependent manner compared with the high glucose group. The expression of ET-1 and HO-1 showed little difference in a time gradient of danshensu(P>0.05).
  
  
  CONCLUSIONS
  Danshensu can protect HPMCs through inhibiting the expression of FN and Col-I induced by high glucose, which is related to the suppression of oxidative stress.
authors.affiliation: Department of Nephrology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.forename: Jianwen
authors.initials: J
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Yi
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Cai
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D010537:Peritoneum / Q000166:cytology*
chemical_list: D024042:Collagen Type I; D004365:Drugs, Chinese Herbal; D005353:Fibronectins; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; D005947:Glucose