title: Development of the novel biotube inserting technique for acceleration of thick-walled autologous tissue-engineered vascular grafts fabrication.
abstract: To accelerate the fabrication of thick-walled autologous tissue-engineered vascular grafts (TEVGs), a novel biotube inserting technique was developed. After 2 weeks of subcutaneous embedding in rabbits, silicone rods (diameter, 3 mm; length, 35 mm) became encapsulated in connective tissues. Single-layered biotubes were obtained after removing the silicone rods. One silicone rod encapsulated in tissues was inserted into a single-layered biotube to form two layers of autologous tubular tissues. Three layers of autologous tubular tissues were also obtained by applying the same technique. Following a 2-week re-embedding procedure, two layers or three layers of autologous tubular tissues were integrated to form two-layered or three-layered TEVGs. Both wall thickness and burst pressure of three-layered TEVGs were significantly higher than those of two-layered and single-layered TEVGs (P < 0.05). The two-layered TEVGs could be applied as small-caliber vascular grafts, and three-layered TEVGs could be applied as medium- or large-caliber vascular grafts.
authors.affiliation: Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Sun
authors.forename: Haifa
authors.initials: H
authors.lastname: Hong
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.forename: Meng
authors.initials: M
authors.lastname: Yin
authors.forename: Jinfen
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D003238:Connective Tissue / Q000378:metabolism; D008422:Materials Testing; D011817:Rabbits; D012828:Silicones / Q000737:chemistry; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D058017:Vascular Grafting / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D012828:Silicones