title: [Inhibitory effect and molecular mechanism of silencing S100A4 gene on the growth of transplanted tumor of human esophageal carcinoma EC-1 cells in nude mice].
abstract: OBJECTIVE
  To study the effect of S100A4siRNA on tumor growth of xenografted human esophageal squamous cell carcinoma (ESCC) cell line EC1 in nude mice and explore its related molecular mechanism.
  
  
  METHODS
  The xenografted tumor model was established in nude mice, and S100A4siRNA chemically synthesized was used to transfect the xenografted nude mice. The tumor growth was observed. The mRNA and protein expressions of S100A4, MMP-2 and E-cadherin in tumor after transfection with S100A4siRNA were detected by RT-PCR and immunohistochemistry.
  
  
  RESULTS
  The tumor volume of S100A4siRNA transfection group was lower than that of nonsense siRNA transfection group and blank control group (P < 0.05), and tumor inhibitive ratio of S100A4siRNA group was higher than that of nonsense siRNA transfection group (P < 0.05). Furthermore, the mRNA and protein expressions of S100A4, MMP-2 in S100A4siRNA group were lower than those in nonsense siRNA group and control group (P < 0.05), the mRNA and protein expressions of E-cadherin in S100A4siRNA group were higher than those in nonsense siRNA group and control group (P < 0.05).
  
  
  CONCLUSIONS
  S100A4siRNA could effectively lead to growth inhibition of xenografted human esophageal tumor in nude mice, with down regulation of MMP-2 and up regulation of E-cadherin, which provides theoretical basis for molecular therapy of ESCC.
authors.affiliation: Henan Key Laboratory of Tumor Pathology, Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Xian-zhao
authors.initials: XZ
authors.lastname: Zheng
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Xuan
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Li
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D020868:Gene Silencing; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000235:genetics*; D014162:Transfection
chemical_list: D015820:Cadherins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C470944:S100a4 protein, mouse; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse