title: The antibacterial effect of gas ozone after 2 months of in vitro evaluation.
abstract: The aim of this study was to evaluate the effect of HealOzone on two microorganisms, 4 and 8 weeks after treatment, using a tooth cavity model. Four groups of caries-free third molars (n = 12) were used (A, B, C and D). Three cavities were prepared into each tooth. After sterilization, groups A and B were inoculated with Streptococcus mutans, and groups C and D, with Lactobacillus casei for 48 h. One cavity of each tooth was used to evaluate the infection. After inoculation, groups B and D were treated with ozone (60 s), and groups A and C were used as controls. Then, the two cavities of each tooth were filled with composite, and the teeth were stored in sucrose medium. The restorations were removed after 4 and 8 weeks, respectively; dentin chips were collected, and the amount of microorganisms was determined. Ozone treatment reduced significantly the amount of S. mutans compared to the control group (p ≤ 0.05). This antibacterial effect was able to be seen after 4 (p = 0.0005) and 8 (p = 0.0002) weeks. No significant difference was found between the control and treated group as far as L. casei is concerned (p > 0.05). HealOzone (60 s) can provide some antibacterial treatment against S. mutans even after 8 weeks. However, an elimination of the microorganisms through HealOzone seems not to be possible. L. casei was more resistant to ozone. Although ozone exerts a significant antibacterial effect against S. mutans, it is probably not enough as the only antibacterial method, during the fillings therapy.
authors.affiliation: Department of Operative Dentistry and Periodontology, Dental School and Hospital, University Medical Center Freiburg, Freiburg, Germany. olga.polydorou@uniklinik-freiburg.de
authors.forename: Olga
authors.initials: O
authors.lastname: Polydorou
authors.forename: Ahmad
authors.initials: A
authors.lastname: Halili
authors.forename: Anette
authors.initials: A
authors.lastname: Wittmer
authors.forename: Klaus
authors.initials: K
authors.lastname: Pelz
authors.forename: Petra
authors.initials: P
authors.lastname: Hahn
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D003188:Composite Resins / Q000737:chemistry; D003731:Dental Caries / Q000382:microbiology; D003764:Dental Materials / Q000737:chemistry; D003793:Dental Restoration, Permanent / Q000379:methods; D003804:Dentin / Q000382:microbiology; D006801:Humans; D007780:Lacticaseibacillus casei / Q000187:drug effects; D008422:Materials Testing; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003188:Composite Resins; D003764:Dental Materials; D010083:Oxidants, Photochemical; D010126:Ozone