title: Polyamine analysis by LC-MS.
abstract: This chapter describes a protocol to analyze polyamines without any derivatization steps utilizing LC-MS/MS. Polyamines are separated by reversed phase LC prior MS analysis using heptafluorobutyric acid as MS compatible volatile ion-pairing agent, and selective and sensitive MS detection is performed using MS/MS in selected reaction monitoring mode.
authors.affiliation: Department of Biosciences, Laboratory of Chemistry, Biocenter Kuopio, University of Kuopio, Kuopio, Finland. merja.hakkinen@uef.fi
authors.forename: Merja R
authors.initials: MR
authors.lastname: Häkkinen
mesh_terms: D002845:Chromatography; D002853:Chromatography, Liquid; D017022:Flow Injection Analysis; D013058:Mass Spectrometry / Q000379:methods*; D011073:Polyamines / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
chemical_list: D011073:Polyamines; D012996:Solutions