title: DNA fingerprinting of septicemic and localized Burkholderia pseudomallei isolates from Malaysian patients.
abstract: We have analysed DNA fingerprinting patterns by pulsed-field gel electrophoresis (PFGE) of 52 unrelated Burkholderia pseudomallei strains isolated from septicemic and localized infections from Malaysian subjects. A total of 38 PFGE types were observed among 36 septicemic and 16 localized strains with no predominant pattern. Type 25 was seen in 2 epidemiologically related strains, suggesting human to human transmission. Twelve PFGE types were shared among 26 strains (21 septicemic and 5 localized) showing close genetic relatedness with coefficient of similarity of 0.81 to 1.0. The other 26 strains (15 septicemic and 11 localized) were unrelated as shown by the similarity coefficient of < 0.8. This study showed that our B. pseudomallei strains in Malaysia were mainly heterogenous with no predominant type both in septicemic or localized strains.
authors.affiliation: Bacteriology Unit, Infectious Diseases Research Center, Institute for Medical Research, Kuala Lumpur, Malaysia. azura@imr.gov.my
authors.forename: M N
authors.initials: MN
authors.lastname: Azura
authors.forename: A
authors.initials: A
authors.lastname: Norazah
authors.forename: A G M
authors.initials: AG
authors.lastname: Kamel
authors.forename: S Ahmad
authors.initials: SA
authors.lastname: Zorin
mesh_terms: D000328:Adult; D000368:Aged; D016957:Burkholderia pseudomallei / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008296:Malaysia; D008297:Male; D008554:Melioidosis / Q000382:microbiology*; D008875:Middle Aged; D018805:Sepsis / Q000382:microbiology*; D055815:Young Adult