title: Improving yield of industrial biomass propagation by increasing the Trx2p dosage.
abstract: The beneficial effect of improving yeast redox response by increasing thioredoxin levels has been shown. Decreased lipid and protein oxidation is reflected in an increased biomass yield. In addition, increased redox defenses like glutathione and ROS scavenging enzymes are observed. Furthermore, the wine produced with the modified strain presented more aromatic compounds than the control strain, and its organoleptic properties increased. Here, we hypothesize that reduced glycolytic enzyme carbonylation can increase not only the glycolytic flux but also, and consequently, the biomass yield in the industrial biomass propagation process. The commercial use of the thioredoxin bioengineered yeast as an antioxidant dietetic supplement is also discussed.
authors.affiliation: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Valencia, Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Gómez-Pastor
authors.forename: Roberto
authors.initials: R
authors.lastname: Pérez-Torrado
authors.forename: Emilia
authors.initials: E
authors.lastname: Matallana
mesh_terms: D057005:Bioengineering; D018533:Biomass; D005285:Fermentation; D018628:Gene Dosage; D015966:Gene Expression Regulation, Fungal; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D013879:Thioredoxins / Q000235:genetics* / Q000378:metabolism*; D014920:Wine / Q000382:microbiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C516110:TRX2 protein, S cerevisiae; D013879:Thioredoxins