title: The conserved factor DE-ETIOLATED 1 cooperates with CUL4-DDB1DDB2 to maintain genome integrity upon UV stress.
abstract: Plants and many other eukaryotes can make use of two major pathways to cope with mutagenic effects of light, photoreactivation and nucleotide excision repair (NER). While photoreactivation allows direct repair by photolyase enzymes using light energy, NER requires a stepwise mechanism with several protein complexes acting at the levels of lesion detection, DNA incision and resynthesis. Here we investigated the involvement in NER of DE-ETIOLATED 1 (DET1), an evolutionarily conserved factor that associates with components of the ubiquitylation machinery in plants and mammals and acts as a negative repressor of light-driven photomorphogenic development in Arabidopsis. Evidence is provided that plant DET1 acts with CULLIN4-based ubiquitin E3 ligase, and that appropriate dosage of DET1 protein is necessary for efficient removal of UV photoproducts through the NER pathway. Moreover, DET1 is required for CULLIN4-dependent targeted degradation of the UV-lesion recognition factor DDB2. Finally, DET1 protein is degraded concomitantly with DDB2 upon UV irradiation in a CUL4-dependent mechanism. Altogether, these data suggest that DET1 and DDB2 cooperate during the excision repair process.
authors.affiliation: Institut de Biologie de l'Ecole Normale Supérieure, Section de Génomique Environnementale et Evolutive, CNRS UMR 8197 INSERM U1021, Paris, France.
authors.forename: Enric
authors.initials: E
authors.lastname: Castells
authors.forename: Jean
authors.initials: J
authors.lastname: Molinier
authors.forename: Giovanna
authors.initials: G
authors.lastname: Benvenuto
authors.forename: Clara
authors.initials: C
authors.lastname: Bourbousse
authors.forename: Gerald
authors.initials: G
authors.lastname: Zabulon
authors.forename: Antoine
authors.initials: A
authors.lastname: Zalc
authors.forename: Stefano
authors.initials: S
authors.lastname: Cazzaniga
authors.forename: Pascal
authors.initials: P
authors.lastname: Genschik
authors.forename: Fredy
authors.initials: F
authors.lastname: Barneche
authors.forename: Chris
authors.initials: C
authors.lastname: Bowler
mesh_terms: D017360:Arabidopsis / Q000502:physiology / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D044842:Cullin Proteins / Q000378:metabolism*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018745:Genome, Plant / Q000528:radiation effects*; D047908:Intracellular Signaling Peptides and Proteins; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; C514806:CULLIN4 protein, Arabidopsis; D044842:Cullin Proteins; C530204:DDB2 protein, Arabidopsis; C088435:DET1 protein, Arabidopsis; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins