title: Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells.
abstract: This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A(2) (PLA(2)), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 µM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA(2) assay, we failed to observe the change of cPLA(2) and sPLA(2) activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Ho Sun
authors.initials: HS
authors.lastname: Song
authors.forename: Hee Rae
authors.initials: HR
authors.lastname: Kim
authors.forename: Myoung Soo
authors.initials: MS
authors.lastname: Ko
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Jeong
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Myung Cheul
authors.initials: MC
authors.lastname: Kim
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Hwang
authors.forename: Uy Dong
authors.initials: UD
authors.lastname: Sohn
authors.forename: Yoon-Myoung
authors.initials: YM
authors.lastname: Gimm
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Myung
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Sim