title: In situ hybridization on brain tissue.
abstract: ABSTRUCT: In situ hybridization (ISH) is an important method for determining the distribution of mRNA within cells or tissue preparations by hybridization of a nucleic acid probe (either DNA or RNA) with a specific target nucleic acid (usually mRNA) (1,2). Thus, ISH enables the localization of transcripts within cells, tissues, and whole body and allows a neuroanatomic comparison of specific mRNA expression with the respective protein expression. Furthermore, ISH can serve as a tool to detect quantitative changes in gene expression in distinct neuroanatomic areas under various experimental conditions.
authors.forename: C H
authors.initials: CH
authors.lastname: Lammers
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Maral Mouradian