title: Using time-resolved fluorescence to measure serum venom-specific IgE and IgG.
abstract: We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.
authors.affiliation: Centre for Clinical Research in Emergency Medicine, Western Australian Institute for Medical Research, University of Western Australia, Perth, Australia. Pauline.vaneeden@uwa.edu.au
authors.forename: Pauline E
authors.initials: PE
authors.lastname: van Eeden
authors.forename: Michael D
authors.initials: MD
authors.lastname: Wiese
authors.forename: Susan
authors.initials: S
authors.lastname: Aulfrey
authors.forename: Belinda J
authors.initials: BJ
authors.lastname: Hales
authors.forename: Shelley F
authors.initials: SF
authors.lastname: Stone
authors.forename: Simon G A
authors.initials: SG
authors.lastname: Brown
mesh_terms: D000485:Allergens / Q000276:immunology; D000862:Ant Venoms / Q000276:immunology*; D001315:Australia; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007299:Insect Bites and Stings / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000485:Allergens; D000862:Ant Venoms; D007074:Immunoglobulin G; D007073:Immunoglobulin E