title: Epithelial-to-mesenchymal transdifferentiation of peritoneal mesothelial cells mediated by oxidative stress in peritoneal fibrosis rats.
abstract: OBJECTIVE
  To investigate the role of oxidative stress in the epithelial-to-mesenchymal transdifferentiation (EMT) of peritoneal mesothelial cells in rat model of peritoneal fibrosis and the effect of probucol on peritoneal fibrosis.
  
  
  METHODS
  The rat model of peritoneal fibrosis was induced by 4.25% high glucose peritoneal dialysis fluid (PDF). The rats were randomly divided into 4 groups:the control group, the saline group, the peritoneal fibrosis group, and the probucol group. A 4 hour peritoneal equilibration test (PET) was performed 4 weeks later. The peritoneal function and net ultrafiltration (UF) volume were determined. The level of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in peritoneal tissue were examined. The histology of peritoneal membrane was evaluated by light microscopy. E-cadherin and α-smooth muscle actin (α-SMA) protein expression was evaluated by immunohistochemical method and Western blot.
  
  
  RESULTS
  The mesothelial cells were detached from peritoneal membrane in peritoneal firbosis rats. Comparing with the control rats, the thickness of visceral peritoneum, the level of MDA, and the-SMA protein expression were increased while the net ultrafiltration volume, the level of GSH-Px and E-cadherin protein expression were decreased in peritoneal firbosis rats. All these changes were reversed in the rats treated with probucol.
  
  
  CONCLUSIONS
  Oxidative stress plays an important role in transdifferentiation of peritoneal mesothelial cell in the peritoneal fibrosis rats. Probucol can improve structure and function of peritoneum, and partially reverse the EMT by reducing the oxidative stress.
authors.affiliation: Department of Nephrology, Central South University, Changsha 410011, China.
authors.forename: Shaobin
authors.initials: S
authors.lastname: Duan
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ling
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000009:adverse effects; D015312:Hemodialysis Solutions / Q000009:adverse effects; D008297:Male; D008648:Mesoderm / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology*; D056627:Peritoneal Fibrosis / Q000139:chemically induced / Q000473:pathology*; D010537:Peritoneum / Q000187:drug effects* / Q000503:physiopathology; D011341:Probucol / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015312:Hemodialysis Solutions; D005947:Glucose; D011341:Probucol