title: Characterization of the active bacterial community involved in natural attenuation processes in arsenic-rich creek sediments.
abstract: Acid mine drainage of the Carnoulès mine (France) is characterized by acid waters containing high concentrations of arsenic and iron. In the first 30 m along the Reigous, a small creek draining the site, more than 38% of the dissolved arsenic was removed by co-precipitation with Fe(III), in agreement with previous studies, which suggest a role of microbial activities in the co-precipitation of As(III) and As(V) with Fe(III) and sulfate. To investigate how this particular ecosystem functions, the bacterial community was characterized in water and sediments by 16S rRNA encoding gene library analysis. Based on the results obtained using a metaproteomic approach on sediments combined with high-sensitivity HPLC-chip spectrometry, several GroEL orthologs expressed by the community were characterized, and the active members of the prokaryotic community inhabiting the creek sediments were identified. Many of these bacteria are β-proteobacteria such as Gallionella and Thiomonas, but γ-proteobacteria such as Acidithiobacillus ferrooxidans and α-proteobacteria such as Acidiphilium, Actinobacteria, and Firmicutes were also detected.
authors.affiliation: Laboratoire HydroSciences Montpellier, UMR5569 (CNRS-IRD-Universités Montpellier I et II), Université Montpellier II, CC MSE, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France. bruneel@msem.univ-montp2.fr
authors.forename: Odile
authors.initials: O
authors.lastname: Bruneel
authors.forename: Aurélie
authors.initials: A
authors.lastname: Volant
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gallien
authors.forename: Bertrand
authors.initials: B
authors.lastname: Chaumande
authors.forename: Corinne
authors.initials: C
authors.lastname: Casiot
authors.forename: Christine
authors.initials: C
authors.lastname: Carapito
authors.forename: Amélie
authors.initials: A
authors.lastname: Bardil
authors.forename: Guillaume
authors.initials: G
authors.lastname: Morin
authors.forename: Gordon E
authors.initials: GE
authors.lastname: Brown
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Personné
authors.forename: Denis
authors.initials: D
authors.lastname: Le Paslier
authors.forename: Christine
authors.initials: C
authors.lastname: Schaeffer
authors.forename: Alain
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: Philippe N
authors.initials: PN
authors.lastname: Bertin
authors.forename: Françoise
authors.initials: F
authors.lastname: Elbaz-Poulichet
authors.forename: Florence
authors.initials: F
authors.lastname: Arsène-Ploetze
mesh_terms: D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D001151:Arsenic