title: Production and characterization of Monoclonal antibodies to bluetongue virus.
abstract: In the present study, a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein, titres, isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones, a majority of them (n = 18) belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA, the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However, this clone showed a variable percent of inhibition ranging from16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones, only 4A10 was found to have a possible diagnostic application.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt.) Uttarakhand, India. bhanu6467@gmail.com
authors.forename: Veerakyathappa
authors.initials: V
authors.lastname: Bhanuprakash
authors.forename: Madhusudhan
authors.initials: M
authors.lastname: Hosamani
authors.forename: Vinayagamurthy
authors.initials: V
authors.lastname: Balamurugan
authors.forename: Pradeep Narayan
authors.initials: PN
authors.lastname: Gandhale
authors.forename: Gnanavel
authors.initials: G
authors.lastname: Venkatesan
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology*; D001820:Bluetongue virus / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral