title: [Anaphylactoid reaction induced by Qingkailing injection via basophils cells degranulation].
abstract: OBJECTIVE
  To observe the effects of Qingkailing injection on RBL-2H3 cell degranulation and histamine release, and discuss the possible mechanism of anaphylactoid reaction induced by Qingkailing injection.
  
  
  METHODS
  RBL-2H3 cells were incubated with Qingkailing injection for 30 min. Then the morphological changes of cells were observed by transmission electron microscopy. Cell degranulation rate was detected by Alcian blue dye assay, Annexin V binding assay and beta-hexosaminidase assay, and cell histamine release rate was detected by ELISA.
  
  
  RESULTS
  Different concentration of Qingkailing injection can induce the typical morphological changes in RBL-2H3 cell with degranulation. The rates of degranulation and histamine release in Qingkailing injection treated cells were significantly increased and dose-dependent.
  
  
  CONCLUSIONS
  RBL-2H3 cell degranulation and histamine release can be induced by single administration of Qingkailing injection, and then induced anaphylactoid reaction, which may be one of the possible mechanisms of serious adverse induced by Qingkailing injection for the first administration in clinic.
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Zheng
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yan
authors.forename: Linglan
authors.initials: L
authors.lastname: Tu
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001491:Basophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D015550:Cell Degranulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006632:Histamine / Q000378:metabolism; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; C504928:Qingkailing; D006632:Histamine