title: Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen.
abstract: Marker removal strategies were developed for Thermoanaerobacterium saccharolyticum to select against the pyrF gene and the pta and ack genes. The pta- and ack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless Δldh Δpta Δack strain that produces ethanol at a high yield.
authors.affiliation: Mascoma Corporation, 67 Etna Rd., Lebanon, NH 03766, USA.
authors.forename: A Joe
authors.initials: AJ
authors.lastname: Shaw
authors.forename: Sean F
authors.initials: SF
authors.lastname: Covalla
authors.forename: David A
authors.initials: DA
authors.lastname: Hogsett
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Herring
mesh_terms: D000084:Acetate Kinase / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D017353:Gene Deletion; D005827:Genetics, Microbial / Q000379:methods*; D008969:Molecular Sequence Data; D010709:Phosphate Acetyltransferase / Q000235:genetics; D017422:Sequence Analysis, DNA; D046950:Thermoanaerobacterium / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000431:Ethanol; D010709:Phosphate Acetyltransferase; D000084:Acetate Kinase