title: [Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro].
abstract: OBJECTIVE
  Through analysis of variation and function of 5 main endogenous hormones in the formation of microtuber of Dioscorea opposite in vitro to explore the physiological and biochemical mechanism of microtuber development.
  
  
  METHODS
  When microtubers were induced on MS + 6-BA 1.5 mg x L(-1) + NAA 1.5 mg x L(-1) + sucrose 5% medium, the endogenous hormones were isolated during different formation stages of microtubers, then purified and detected with enzyme-linked immunosorbent assays (ELISA).
  
  
  RESULTS
  The results showed that GA3 slightly decreased in initial period, rose suddenly 20 days later, and than decreased. IAA showed a dropping tendency in the total course, ABA and ZR increased in a long period, dropped at last. JA continuously rose and never dropped, GA3 and ABA and the ratio of GA3 and JA varied obviously.
  
  
  CONCLUSIONS
  IAA, ABA, JA , ZR and GA3 play an important role in controlling formation of microtubers in D. opposite in vitro.
authors.affiliation: School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chang
authors.forename: Jianping
authors.initials: J
authors.lastname: Xue
authors.forename: Yunxian
authors.initials: Y
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Conghui
authors.initials: C
authors.lastname: Xiong
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D027742:Dioscorea / Q000254:growth & development / Q000378:metabolism*; D005875:Gibberellins / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D035281:Plant Tubers / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D005875:Gibberellins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; C030737:indoleacetic acid; D000040:Abscisic Acid; D007541:Isopentenyladenosine; C009699:zeatin riboside; C007842:gibberellic acid