title: [Effects of HSYA on expression of bFGF protein and MMP-9 in BGC-823 transplantation tumor of nude mice].
abstract: OBJECTIVE
  To investigate the effects of hydroxy safflor yellow A (HSYA) on the expression of bFGF protein and MMP-9 mRNA or protein of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice.
  
  
  METHODS
  The BGC-823 cells were subcutaneously injected into the right anterior armpit of BALB/C nu/nu nude mice, and the animal model of transplantation tumor was established. The experimental groups were treated with HSYA at concentration of 0.056 and 0.028 g x L(-1) and cyclophosphamide at 2 g x L(-1), or with physiologic saline. The tumor inhibitory effect was observed, and the mRNA expression of MMP-9 of transplantation tumor was detected by real time-fluorescent quantitation PCR and the protein expression of MMP-9 and bFGF were detected by enzyme linked immunosorbent assay.
  
  
  RESULTS
  The IR in the group with HSYA at the concentration of 0.028 g x L(-1) is higher than in the group with normal sodium. After treatment with HSYA, the mRNA expression of MMP-9 has significant difference at the concentration of 0.028 g x L(-1) as compared with physiologic saline-treated group (P < 0.05), but the protein expression of MMP-9 and bFGF is obviously less than that in the physiologic saline-treated group (P < 0.05).
  
  
  CONCLUSIONS
  The possible mechanism of HSYA in given concentration to antagonize tumor angiogenesis may be related with inhibiting the protein expression of MMP-9 and bFGF or the mRNA expression of MMP-9 in tumor tissue to reduce the degradation of blood vessel basilar membrane, and to restrain the migration of blood vessel and decrease the tumor vascularization.
authors.affiliation: Department of Traditional Chinese Medicine of Medical College of Xiamen University, Xiamen 361005, China. xishengyan13204@163.com
authors.forename: Shengyan
authors.initials: S
authors.lastname: Xi
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Xie
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yue
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Baoxia
authors.initials: B
authors.lastname: Zang
authors.forename: Xuemin
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; D016222:Fibroblast Growth Factor 2; D020780:Matrix Metalloproteinase 9