title: High-risk human papillomavirus reduces the expression of microRNA-218 in women with cervical intraepithelial neoplasia.
abstract: This study was designed to investigate whether there is a correlation between the down-regulation of microRNA-218 (miR-218) and the presence of human papillomavirus (HPV) infection in the pathogenesis of cervical cancer. The participants comprised 78 women with cervical intraepithelial neoplasia (CIN); 22 (28.2%) had CIN 1, 27 (34.6%) had CIN 2 and 29 (37.2%) had CIN 3. MiR-218 expression was determined by reverse transcriptase polymerase chain reaction and HPV genotypes in tissue specimens were identified with a microarray test kit. The findings showed that miR-218 levels in patients with high-risk HPV infection were lower than in those infected with low-risk or intermediate-risk HPV, or in those who were HPV-free. MiR-218 levels in patients with high-risk CIN were lower than in those with low-risk CIN. We concluded that infection with high-risk HPV lowered the expression of miR-218 and that down-regulation of miR-218 was involved in the pathogenesis of cervical cancer.
authors.affiliation: Department of Clinical Laboratory Medicine, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Yuan
authors.forename: B
authors.initials: B
authors.lastname: Cui
authors.forename: X
authors.initials: X
authors.lastname: Zou
authors.forename: Y
authors.initials: Y
authors.lastname: Qiao
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; C547471:MIRN218 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm