title: Isolation and characterization of CcAbf62A, a GH62 α-L-arabinofuranosidase, from the basidiomycete Coprinopsis cinerea.
abstract: A cDNA encoding a putative extracellular α-L-arabinofuranosidase was cloned from the basidiomycete Coprinopsis cinerea (CcAbf62A). CcAbf62A belongs to glycoside hydrolase family 62 (GH62), but is phylogenetically distinct from previously characterized GH62 enzymes. The recombinant CcAbf62A, expressed in Pichia pastoris, released L-arabinose from both wheat arabinoxylan and oat-spelt xylan. The enzyme activity for wheat arabinoxylan was increased by the addition of CcEst1, a carbohydrate esterase from C. cinerea.
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan. khashi@cc.tuat.ac.jp
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshida
authors.forename: Keiji
authors.initials: K
authors.lastname: Hasumi
mesh_terms: D000363:Agaricales / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001089:Arabinose / Q000378:metabolism; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D018076:DNA, Complementary; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase