title: Rapid method for the determination of organochlorine pesticides and PCBs in fish muscle samples by microwave-assisted extraction and analysis of extracts by GC-ECD.
abstract: A procedure for the multiresidue determination of organochlorine pesticides and polychlorinated biphenyls in fish muscle samples has been developed. The method is based on the microwave-assisted extraction (MAE) of food samples from an acetonitrile-water (95 + 5, v/v) mixture followed by SPE cleanup of the extracts and analysis by GC with an electron capture detector. MAE operational parameters, such as the extraction solvent, temperature, and time, were optimized with respect to the extraction efficiency of the target compounds from food samples with 10-13% fat content. The chosen extraction technique allows reduction of the solvent consumption and extraction time when compared with methods already used. Acetonitrile is a good extraction solvent for low-fat matrixes (2-20% fat content), such as fish samples, because it does not significantly dissolve the highly polar proteins, salts, and sugars commonly found in food and gives high recoveries of a wide polarity range of analytes. For purification, SPE using LC-Florisil was shown to be sufficient for the removal of coextracted substances. Recoveries > 78% with RSD values < 15% were obtained for all compounds under the selected conditions. Method quantification limits were in the 5-10 microg/kg range. The method was applied to the analysis of samples of herring (Clupea harengus) purchased at the local fish market. The method is rapid and reliable for the determination of organochlorine analytes in fish muscle.
authors.affiliation: Gdansk University of Technology, Department of Analytical Chemistry, Chemical Faculty, ul. Narutowicza 11/12, 80-233 Gdańsk, Poland. angelika.beyer@gmail.com
authors.forename: Angelika M
authors.initials: AM
authors.lastname: Wilkowska
authors.forename: Marek
authors.initials: M
authors.lastname: Biziuk
mesh_terms: D000818:Animals; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008872:Microwaves; D018482:Muscle, Skeletal / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D012997:Solvents; D014020:Tissue Extracts / Q000032:analysis
chemical_list: D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D007202:Indicators and Reagents; D010575:Pesticides; D012997:Solvents; D014020:Tissue Extracts; D011078:Polychlorinated Biphenyls