title: Crystal structure of the Nogo-receptor-2.
abstract: The inhibition of axon regeneration upon mechanical injury is dependent on interactions between Nogo receptors (NgRs) and their myelin-derived ligands. NgRs are composed of a leucine-rich repeat (LRR) region, thought to be structurally similar among the different isoforms of the receptor, and a divergent "stalk" region. It has been shown by others that the LRR and stalk regions of NgR1 and NgR2 have distinct roles in conferring binding affinity to the myelin associated glycoprotein (MAG) in vivo. Here, we show that purified recombinant full length NgR1 and NgR2 maintain significantly higher binding affinity for purified MAG as compared to the isolated LRR region of either NgR1 or NgR2. We also present the crystal structure of the LRR and part of the stalk regions of NgR2 and compare it to the previously reported NgR1 structure with respect to the distinct signaling properties of the two receptor isoforms.
authors.affiliation: Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York 10065, USA.
authors.forename: Mariya
authors.initials: M
authors.lastname: Semavina
authors.forename: Nayanendu
authors.initials: N
authors.lastname: Saha
authors.forename: Momchil V
authors.initials: MV
authors.lastname: Kolev
authors.forename: Yehuda
authors.initials: Y
authors.lastname: Goldgur
authors.forename: Roman J
authors.initials: RJ
authors.lastname: Giger
authors.forename: Juha P
authors.initials: JP
authors.lastname: Himanen
authors.forename: Dimitar B
authors.initials: DB
authors.lastname: Nikolov
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D058851:GPI-Linked Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000378:metabolism; D000070816:Nogo Receptor 1; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D058851:GPI-Linked Proteins; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D000070816:Nogo Receptor 1; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D011994:Recombinant Proteins; C486581:Rtn4r protein, rat