title: Comparison of two sample preparation methods for analysis of organochlorine pesticides residues in fish muscle.
abstract: The aim of this study was to compare recoveries of organochlorine pesticides (heptachlor, α-HCH, β-HCH, γ-HCH, op'-DDD, pp'-DDD, pp'-DDE, op'-DDT, pp'-DDT) from fish muscle dried by two alternative methods: (i) grinding with anhydrous sodium sulphate and (ii) freeze drying. Pesticide residues content was determined by gas chromatography-mass spectrometry (GS-MS) method. For four pesticides (γ-HCH, α-HCH, heptachlor and pp;-DDD) in four of five fish species, higher recoveries were obtained from the freeze-dried samples. For five remaining pesticides, correlations between fish species and drying method were not found. The results of this study do not clearly indicate which drying method caused lower losses of analytes. Recoveries from the freeze-dried samples ranged from 69.9 to 117.6 %, while recoveries from the samples ground with sodium sulphate varied from 64.4 to 126.7 %. Either of the methods gave satisfactory recoveries and they both can be used interchangeably.
authors.affiliation: Department of Toxicology, West Pomeranian University of Technology, Szczecin, Poland. Agata.Witczak@zut.edu.pl
authors.forename: Agata
authors.initials: A
authors.lastname: Witczak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Patrzykąt
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D005612:Freeze Drying / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006843:Hydrocarbons, Chlorinated / Q000032:analysis / Q000302:isolation & purification*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D006843:Hydrocarbons, Chlorinated; D010573:Pesticide Residues