title: Biomimetic high density lipoprotein nanoparticles for nucleic acid delivery.
abstract: We report a gold nanoparticle-templated high density lipoprotein (HDL AuNP) platform for gene therapy that combines lipid-based nucleic acid transfection strategies with HDL biomimicry. For proof-of-concept, HDL AuNPs are shown to adsorb antisense cholesterylated DNA. The conjugates are internalized by human cells, can be tracked within cells using transmission electron microscopy, and regulate target gene expression. Overall, the ability to directly image the AuNP core within cells, the chemical tailorability of the HDL AuNP platform, and the potential for cell-specific targeting afforded by HDL biomimicry make this platform appealing for nucleic acid delivery.
authors.affiliation: Department of Urology, Feinberg School of Medicine, Northwestern University , 303 East Chicago Avenue, Tarry 16-703, Chicago, Illinois 60611, United States.
authors.forename: Kaylin M
authors.initials: KM
authors.lastname: McMahon
authors.forename: R Kannan
authors.initials: RK
authors.lastname: Mutharasan
authors.forename: Sushant
authors.initials: S
authors.lastname: Tripathy
authors.forename: Dorina
authors.initials: D
authors.lastname: Veliceasa
authors.forename: Mariana
authors.initials: M
authors.lastname: Bobeica
authors.forename: Dale K
authors.initials: DK
authors.lastname: Shumaker
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: Luthi
authors.forename: Brian T
authors.initials: BT
authors.lastname: Helfand
authors.forename: Hossein
authors.initials: H
authors.lastname: Ardehali
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
authors.forename: Olga
authors.initials: O
authors.lastname: Volpert
authors.forename: C Shad
authors.initials: CS
authors.lastname: Thaxton
mesh_terms: D002460:Cell Line; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D018716:Molecular Mimicry; D053758:Nanoparticles; D009696:Nucleic Acids / Q000008:administration & dosage*
chemical_list: D008075:Lipoproteins, HDL; D009696:Nucleic Acids