title: Synthesis, characterization and osteoconductivity properties of bone fillers based on alendronate-loaded poly(ε-caprolactone)/hydroxyapatite microspheres.
abstract: A superior drug controlled release system capable of achieving efficient osteogenesis is in imperative demand because of limited bone substitute tissue for the treatment of bone defect. In the present study, we investigated the potential of using poly(ε-caprolactone)-hydroxyapatite (PCL-HA) composite microspheres as an injectable bone repair vehicle by controlled release of alendronate (AL), a medicine that belongs to the bisphosphonates family. The PCL/HA-AL microspheres were prepared with solid/oil/water emulsion technique, which included two processes: (1) AL was loaded on the hydroxyapatite nanoparticles; (2) the HA-AL complex was built in the PCL matrix. The spherical PCL/HA-AL microspheres were characterized with its significantly improved encapsulation efficiency of hydrophilic AL and better sustained release. Human bone mesenchymal stem cells (hMSCs) were cultured on the surface of these microspheres and exhibited high proliferative profile. Specifically, in osteogenic medium, hMSCs on the surface of PCL/HA-AL microspheres displayed superior osteogenic differentiation which was verified by alkaline phosphatase activity assay. In conclusion, by presenting strong osteogenic commitment of hMSCs in vitro, the PCL/HA-AL microspheres have the potential to be used as an injectable vehicle for local therapy of bone defect.
authors.affiliation: Department of Stomatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Liangqing
authors.initials: L
authors.lastname: Hong
authors.forename: You
authors.initials: Y
authors.lastname: Ling
authors.forename: Jun
authors.initials: J
authors.lastname: Pang
authors.forename: Youqiang
authors.initials: Y
authors.lastname: Fang
authors.forename: Kun
authors.initials: K
authors.lastname: Wei
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
mesh_terms: D019386:Alendronate / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D049109:Cell Proliferation; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008863:Microspheres; D010012:Osteogenesis; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite; D000469:Alkaline Phosphatase; D019386:Alendronate