title: Attenuation of hyperoxia-induced lung injury in rats by adrenomedullin.
abstract: Oxidative stress and inflammation are involved in the pathogenesis of acute lung injury (ALI). Adrenomedullin (AM) is an endogenous peptide with anti-inflammatory and antioxidant properties. This study investigated that whether AM treatment may ameliorate hyperoxia-induced ALI in rats via inhibition of oxidative stress and inflammation. Rats were randomized to receive continuous intravenous infusion of AM or saline through a microosmotic pump, and then ALI was induced by exposing the animals in sealed cages >95% oxygen for 72 h. Exposure to hyperoxia caused lung injury as increased infiltration of inflammatory cells and disruption of lung architecture. AM administration markedly improved these changes. Additionally, AM administration significantly increased glutathione peroxidase and superoxide dismutase activities. Meanwhile, hyperoxia-induced increase of lipid hydroperoxide level was markedly reduced by AM treatment. Moreover, nuclear factor-kappa B-DNA-binding activity, and production of the inflammatory mediators interleukin-6, keratinocyte-derived chemokine, and matrix metalloproteinase 9, were significantly inhibited by AM treatment. AM ameliorates hyperoxia-induced ALI in rats by suppression of oxidative stress and inflammation.
authors.affiliation: Department of Thoracic Surgery, Subei People's Hospital of Jiangsu province, Jiangsu, 225001, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tao
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Shu
authors.forename: Qian-Bing
authors.initials: QB
authors.lastname: Miao
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Zhu
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000209:etiology; D053607:Adrenomedullin / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D018925:Chemokines / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D018496:Hyperoxia / Q000150:complications*; D007249:Inflammation / Q000188:drug therapy; D015850:Interleukin-6 / Q000096:biosynthesis; D008054:Lipid Peroxides / Q000096:biosynthesis / Q000378:metabolism; D008168:Lung / Q000187:drug effects; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase
chemical_list: D018925:Chemokines; D015850:Interleukin-6; D008054:Lipid Peroxides; D016328:NF-kappa B; C074302:keratinocyte-derived chemokines; D053607:Adrenomedullin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D020780:Matrix Metalloproteinase 9