title: Effect of temperature on growth of the pathogenic oomycete Pythium insidiosum.
abstract: Pythium insidiosum causes a potentially life-threatening infectious disease called pythiosis. An early, accurate diagnosis is important, since prompt treatment leads to a better prognosis. Unsuccessful attempts to isolate the organism have been associated with specimens subjected to lower temperatures. We analyzed growth of P. insidiosum at various temperatures. Culture at low (8 degrees C) and high (42 degrees C) temperatures resulted in death or inhibited growth of the organism. Culture under optimal temperatures (28 and 32 degrees C) was important for successful isolation of P. insidiosum.
authors.affiliation: Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. mr_en@hotmail.com
authors.forename: Theerapong
authors.initials: T
authors.lastname: Krajaejun
authors.forename: Piriyaporn
authors.initials: P
authors.lastname: Chongtrakool
authors.forename: Kanong
authors.initials: K
authors.lastname: Angkananukul
authors.forename: Tristan T
authors.initials: TT
authors.lastname: Brandhorst
mesh_terms: D003080:Cold Temperature; D006358:Hot Temperature; D006801:Humans; D011775:Pythium / Q000254:growth & development* / Q000302:isolation & purification