title: [Simultaneously determination of atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin in pain-relieving plaster for arthritis by RP-HPLC].
abstract: OBJECTIVE
  To establish RP-HPLC method for determination of atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin in pain-relieving plaster for arthritis.
  
  
  METHODS
  The sample were separated on an Alltima C18 Column (4.6 mm x 250 mm, 5 microm) with the moblie phase of CH3 CN-0.1% H3 PO4. Flow rate was 1 mL x min(-1). The detective wavelength was set at 210 and 280 nm. Column temperature was 30 degrees C.
  
  
  RESULTS
  The calibration curve for atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin revealed linearity in the range of 2.01-50.25, 15.08-377.00, 5.02-125.50, 5.03-125.75 mg x L(-1), respectively. The recoveries of atropine sulphate, diphenhydramine hydrochloride, capsaicin and dihydrocapsaicin were 99.00% with RSD of 0.95%, 99.89% with RSD of 1.2%, 100.1% with RSD of 1.5% and 99.51%, with RSD of 1.4%, respectively.
  
  
  CONCLUSIONS
  The method is simple, rapid and accurate, which is suitable for the quality control of pain-relieving plaster for arthritis.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. jxiaobin2005@hotmail.com
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Wei
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Yiping
authors.initials: Y
authors.lastname: Chu
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001168:Arthritis / Q000188:drug therapy*; D001285:Atropine / Q000032:analysis* / Q000627:therapeutic use; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004155:Diphenhydramine / Q000032:analysis* / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000627:therapeutic use; D006801:Humans; D010146:Pain / Q000188:drug therapy*
chemical_list: D004365:Drugs, Chinese Herbal; D001285:Atropine; D004155:Diphenhydramine; D002211:Capsaicin; C012906:dihydrocapsaicin