title: Precolumn derivatization followed by liquid chromatographic separation and determination of tramiprosate in rat plasma by fluorescence detector: application to pharmacokinetics.
abstract: Alzheimer disease (AD) is characterized pathologically by extracellular amyloid deposits composed of amyloid β (Aβ) protein. A simple and rapid method using HPLC with fluorescence detector was developed and validated for determination of tramiprosate in rat plasma. Pre-column derivatization of the deproteinized rat plasma was carried out using o-phthaldialdehyde (OPA) as a fluorescent reagent in presence of 3-mercaptopropionic acid. The liquid chromatographic separation was achieved on a Kromasil C18 column using methanol:acetonitrile: 20 mM phosphate buffer pH 7.5 (8.0:17.5:74.5 v/v/v) as a mobile phase in an isocratic elution mode. The eluents were monitored by a fluorescence detector set at 330 and 450 nm of excitation and emission wavelength respectively. Vigabatrin was used as an internal standard. The method was linear within the range 30.0-1000.0 ng/mL. Design of experiments (DOE) was used to evaluate the robustness of the method. The developed method was applied to study the pharmacokinetics of tramiprosate in rats.
authors.affiliation: HPLC/UV Group, Analytical Chemistry Division, Discovery Laboratory Indian Institute of Chemical Technology (IICT), Tarnaka, Hyderabad 500607, India. rnrao55@yahoo.com
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Maurya
authors.forename: Dhananjay D
authors.initials: DD
authors.lastname: Shinde
authors.forename: Sara
authors.initials: S
authors.lastname: Khalid
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013654:Taurine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D000927:Anticonvulsants; D013654:Taurine; C001355:tramiprosate