title: Nucleoporin MOS7/Nup88 contributes to plant immunity and nuclear accumulation of defense regulators.
abstract: Controlled nucleocytoplasmic trafficking is an important feature for fine-tuning signaling pathways in eukaryotic organisms. Nuclear pore complexes (NPCs) composed of nucleoporin proteins (Nups) are essential for the exchange of macromolecules across the nuclear envelope. A recent genetic screen in our laboratory identified a partial loss-of-function mutation in Arabidopsis MOS7/Nup88 that causes defects in basal immunity, Resistance (R) protein-mediated defense and systemic acquired resistance. In Drosophila and mammalian cells, exportin-mediated nuclear export of activated Rel/NFκB transcription factors is enhanced in nup88 mutants resulting in immune response failure. Consistent with Nup88 promoting nuclear retention of NFκB, our functional analyses revealed that MOS7/Nup88 is required for appropriate nuclear accumulation of the autoactivated R protein snc1, as well as the key immune regulators EDS1 and NPR1. These results suggest that controlling the nuclear concentrations of specific immune regulators is fundamental for defining defense outputs.
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Marcel
authors.initials: M
authors.lastname: Wiermer
authors.forename: Hugo
authors.initials: H
authors.lastname: Germain
authors.forename: Yu Ti
authors.initials: YT
authors.lastname: Cheng
authors.forename: Ana V
authors.initials: AV
authors.lastname: García
authors.forename: Jane E
authors.initials: JE
authors.lastname: Parker
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D017360:Arabidopsis / Q000276:immunology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000276:immunology / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D057865:Plant Immunity
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C118837:EDS1 protein, Arabidopsis; D016328:NF-kappa B; C105496:NPR1 protein, Arabidopsis; D028861:Nuclear Pore Complex Proteins; C482739:SNC1 protein, Arabidopsis; C491301:mbo protein, Drosophila