title: Application of a liquid chromatographic/tandem mass spectrometric method to a kinetic study of derivative glucosamine in healthy human urine.
abstract: A sensitive, selective and efficient liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method was developed and validated for the determination of glucosamine in healthy human urine. Urine samples were extracted by acetonitrile and derivatized with o-phthalaldehyde/3-mercaptopropionic acid. Analysis was then carried out using ESI source and methanol/0.2% ammonium acetate-0.1% formic acid mobile phase gradient elution, with tolterodine tartrate as the internal standard. The linear calibration curve ranged from 0.41μg/ml to 82.7μg/ml. The intra-day and inter-day precisions were less than 3.93% and 10.0%, respectively. The extraction recoveries determined at three concentration levels were higher than 88.6%. The method was successfully applied for determining the urine concentration of glucosamine up to 24h after oral administration of 1g glucosamine sulfate dispersible table (containing 785.08mg glucosamine) from a clinical pharmacokinetic study in healthy volunteers.
authors.affiliation: Department of Pharmacy, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Guan
authors.forename: Yun
authors.initials: Y
authors.lastname: Tian
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Zhifu
authors.initials: Z
authors.lastname: Yang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jia
authors.forename: Taijun
authors.initials: T
authors.lastname: Hang
authors.forename: Aidong
authors.initials: A
authors.lastname: Wen
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D000328:Adult; D053000:Analytic Sample Preparation Methods; D001711:Biotransformation; D050071:Bone Density Conservation Agents / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005260:Female; D005944:Glucosamine / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D010003:Osteoarthritis / Q000188:drug therapy; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013678:Technology, Pharmaceutical; D009764:o-Phthalaldehyde / Q000737:chemistry
chemical_list: D050071:Bone Density Conservation Agents; D007202:Indicators and Reagents; D009764:o-Phthalaldehyde; D015097:3-Mercaptopropionic Acid; D005944:Glucosamine