title: Total immunoglobulin G and IgG1 subclass levels specific for the MSP-1(19) of Plasmodium falciparum are different in individuals with either processing-inhibitory, blocking or neutral antibodies.
abstract: BACKGROUND
  Some MSP-1(19) specific antibodies that inhibit merozoite invasion also inhibit the secondary processing of MSP-1. However the binding of these inhibitory antibodies can be blocked by another group of antibodies, called blocking antibodies, which recognize adjacent or overlapping epitopes, but themselves have no effect on either MSP-1 processing or merozoite invasion. These antibodies have been reported to be present in individuals living in a malaria endemic area.
  
  
  METHODS
  Blood samples were obtained from children shown to have processing inhibitory, blocking, and neutral antibodies in a previous study. Enzyme linked immunosorbent assay (ELISA), was used to determine the total IgG, IgM and IgG subtypes.
  
  
  RESULTS
  There was a significant difference in anti-MSP-1(19) IgG, while there was no significant difference in the anti-MSP-1(19) IgM. Only anti MSP-1(19) IgG1, amongst the IgG subtypes was significantly different between the groups.
  
  
  CONCLUSIONS
  This study shows that antibodies against MSP-1 are different not only in specificity and function but also in the amount of total IgG and IgG subtype produced.
authors.affiliation: Department of Biotechnology, College of Food Sciences, Bells University of Technology, Ota, Ogun State, Nigeria. yusufomosun@yahoo.com
authors.forename: Y O
authors.initials: YO
authors.lastname: Omosun
authors.forename: S
authors.initials: S
authors.lastname: Adoro
authors.forename: C I
authors.initials: CI
authors.lastname: Anumudu
authors.forename: A
authors.initials: A
authors.lastname: Odaibo
authors.forename: A A
authors.initials: AA
authors.lastname: Holder
authors.forename: M
authors.initials: M
authors.lastname: Nwagwu
authors.forename: R I
authors.initials: RI
authors.lastname: Nwuba
mesh_terms: D000293:Adolescent; D000818:Animals; D019138:Antibodies, Blocking / Q000276:immunology* / Q000378:metabolism; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology* / Q000378:metabolism; D007223:Infant; D007231:Infant, Newborn; D017780:Malaria Vaccines; D016778:Malaria, Falciparum / Q000097:blood / Q000276:immunology / Q000469:parasitology; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000276:immunology* / Q000378:metabolism; D010963:Plasmodium falciparum / Q000276:immunology* / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D000913:Antibodies, Protozoan; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1