title: The promotion of neurological recovery in an intracerebral hemorrhage model using fibrin-binding brain derived neurotrophic factor.
abstract: Brain derived neurotrophic factor (BDNF) has been shown to ameliorate recovery after intracerebral hemorrhage (ICH). The injured brain tissue after ICH is surrounded by hematoma formed from hemorrhage. Fibrin is abundant in hematoma, which could be a binding target for BDNF. In this work, we have fused a fibrin-binding domain (FBD) to BDNF (FBD-BDNF), and results demonstrate that FBD-BDNF has specific binding ability to fibrin and is retained in hematoma. Using the rat ICH model induced by bacterial collagenase, injected FBD-BDNF has been concentrated and retained at the hematoma. FBD has facilitated BDNF to exert targeting neuroprotective effect to the injured brain tissue around the hematoma after ICH. FBD-BDNF has significantly reduced the hemotoma volume, reduced tissue loss, promoted neural regeneration, and improved the rat behavioral performance.
authors.affiliation: Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Qian Qian
authors.initials: QQ
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Zhi Feng
authors.initials: ZF
authors.lastname: Xiao
authors.forename: Jing Chun
authors.initials: JC
authors.lastname: Huang
authors.forename: Hong Bin
authors.initials: HB
authors.lastname: Ni
authors.forename: Jie
authors.initials: J
authors.lastname: Kong
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Wei Bang
authors.initials: WB
authors.lastname: Liang
authors.forename: Jian Wu
authors.initials: JW
authors.lastname: Dai
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002543:Cerebral Hemorrhage / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D005337:Fibrin / Q000378:metabolism*; D006406:Hematoma / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D009043:Motor Activity / Q000187:drug effects; D009416:Nerve Regeneration / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003692:Delayed-Action Preparations; D005337:Fibrin