title: Longitudinal evaluation of immunohistochemical findings of milk aspiration: an experimental study using a murine model.
abstract: To examine the longitudinal change of pathological findings of the lung and other organs in milk aspiration, an experimental study using a murine model was carried out. Either 0.5 or 1.0 ml cow's milk was instilled into the trachea of rats. From immediately after to 14 days after instillation, the animals were sacrificed, and the lungs, liver, kidneys, and spleen were removed. The results of immunostaining with anti-human α lactalbumin antibody indicated that not only the lung but also the kidney and spleen showed a positive reaction against the antibody over time. Experimentally aspirated milk was detectable in alveoli until 2 days after instillation. It was also detectable in renal tubules from 1 to 6h after instillation. Macrophages containing granules of aspirated milk were observed in splenic red pulp from 3h to 14 days after instillation. Detection of aspirated milk in other organs except the lung would be clear evidence of intravital milk aspiration and would suggest previous or recurrent milk aspiration.
authors.affiliation: Department of Forensic Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Nagai
authors.forename: Miwako
authors.initials: M
authors.lastname: Aoyagi
authors.forename: Eriko
authors.initials: E
authors.lastname: Ochiai
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sakai
authors.forename: Kyoko
authors.initials: K
authors.lastname: Maruyama-Maebashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukui
authors.forename: Kimiharu
authors.initials: K
authors.lastname: Iwadate
mesh_terms: D000818:Animals; D000906:Antibodies; D007150:Immunohistochemistry; D007668:Kidney / Q000276:immunology / Q000473:pathology*; D007768:Lactalbumin / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008892:Milk; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D053120:Respiratory Aspiration / Q000473:pathology*; D013154:Spleen / Q000276:immunology / Q000473:pathology*
chemical_list: D000906:Antibodies; D007768:Lactalbumin