title: DNA intercalating studies of [Ru(bpy)2dmt]2+ with two vacant nitrogen atoms by introducing copper(II) ions.
abstract: A novel, yet effective method for identifying DNA-binding modes of [Ru(bpy)(2)dmt](2+) (where bpy = 2,2'-bipyridine and dmt = 2,3-dimethyl-1,4,8,9-tetra-aza-triphenylene) on an indium tin oxide electrode has been successfully developed by introducing Cu(2+) ion and ethylenediaminetetraacetic acid. The results from emission spectra and fluorescence microscopic images suggested that [Ru(bpy)(2)dmt](2+) not only associates with Cu(2+) ion in both the absence and presence of DNA but also shows strong affinity with DNA in the presence of Cu(2+). Evidence for the strong binding of [Ru(bpy)(2)dmt](2+) to DNA was determined from the interface studies using electrochemical methods. The present study suggests that a combination of photoluminescence measurement with electrochemical methods identifies the DNA-binding behavior of luminescent molecules with redox activities. [Ru(bpy)(2)dmt](2+) binds to DNA via an intercalative mode.
authors.affiliation: Key Laboratory of Technology on Electrochemical Energy Storage and Power Generation in Guangdong University, School of Chemistry and Environment, South China Normal University, Guangzhou, People's Republic of China.
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Guo
authors.forename: Miao-Jing
authors.initials: MJ
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Zheng-He
authors.initials: ZH
authors.lastname: Xu
mesh_terms: D000818:Animals; D001665:Binding Sites; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D055664:Electrochemical Techniques; D066298:In Vitro Techniques; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008856:Microscopy, Fluorescence; D009584:Nitrogen / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D012428:Ruthenium / Q000737:chemistry / Q000494:pharmacology*; D013053:Spectrophotometry
chemical_list: D007364:Intercalating Agents; D009942:Organometallic Compounds; D003300:Copper; D012428:Ruthenium; D004247:DNA; D009584:Nitrogen