title: [The role of angelica injection on P-selectin and anti-cardiolipin antibodies in acute pulmonary embolism rats].
abstract: OBJECTIVE
  To study the effect of Chinese medicine, Angelica, injection on the expression of P-, E-selectin and anti-cardiolipin antibody in acute pulmonary embolism rats.
  
  
  METHODS
  SD rats were randomly divided into 3 groups: normal control group(Group N), thromboembolism group (Group T), and treatment group of thromboembolism with angelica injection (Group TA). There were three time points in every group: 1 h, 4 h and 8 h. Plasma was detected by P-, with 4% paraformaldehyde, and paraffin embedded sections were detected by immunohistochemistry for P-, E-selectin and anti-cardiolipin antibodies.
  
  
  RESULTS
  With HE stain, the inflammatory cells in the lung of rats were relatively rare in every time point in normal control group. In group T and group TA, the inflammatory cells were increasing in every time point in comparison to group N (P < 0.05) and the inflammatory cells were increasing with time in group T. The data revealed that the plasmic level of P-, E-selectin was significantly higher than that in group T1, group T4, group T8 in comparison to the corresponding sub groups of group N (P < 0.05), while it was significantly lower than that in group TA1, group TA4, group TA8 in comparison to the corresponding sub groups of group T (P < 0.05); For the OD value of plasmic anti-cardiolipin antibodies (ACA), no significant difference was observed during was lower expressed by immunohistochemistry.
  
  
  CONCLUSIONS
  Acute pulmonary embolism can lead to infiltration of inflammatory cell in rat lungs. The lung inflammation of acute pulmonary embolism rats can be enhanced probably by the increased release of P-, E-selectin and anti-cardiolipin antibodies, and the enhanced inflammation promotes the release of a series of inflammatory mediators, which exacerbate the injury of lung. Angelica injection relieves the lung inflammation of acute pulmonary embolism rats possibly by inhibiting the expression of P-, E-selectin and anti-cardiolipin antibody, thus playing a role in reducing thrombogenesis.
authors.affiliation: Department of Respiration, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Li
authors.forename: Liang-Xing
authors.initials: LX
authors.lastname: Wang
authors.forename: Li-Fei
authors.initials: LF
authors.lastname: Shen
mesh_terms: D029969:Angelica; D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007267:Injections; D008297:Male; D019007:P-Selectin / Q000378:metabolism*; D011014:Pneumonia / Q000378:metabolism; D011655:Pulmonary Embolism / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017153:Antibodies, Anticardiolipin; D004365:Drugs, Chinese Herbal; D019007:P-Selectin