title: Prevalence of A1555G mitochondrial mutation in Chinese newborns and the correlation with neonatal hearing screening.
abstract: OBJECTIVE
  To investigate the feasibility of genetic screening for deafness causative genes in the process of newborn hearing screening in China.
  
  
  METHODS
  Total 865 newborn babies between November 2009 and March 2010 were enrolled for the simultaneous hearing and deafness causative gene screening in Tongji Hospital, Wuhan, China. Hearing screening followed a two-stage strategy with transient evoked otoacoustic emissions. Infants referred after the second-stage screening were tested by diagnostic auditory brainstem response (ABR). Genomic DNA was extracted from heel blood of newborns, and the mitochondrial 12S rRNA A1555G mutation was detected by polymerase chain reaction (PCR) based restriction fragment length polymorphism and confirmed by DNA sequencing.
  
  
  RESULTS
  In hearing screening, 134 out of the 865 newborns (15.5%) were referred after the first-stage screening and 86.6% (116/134) of them returned for the second stage. After the second-stage screening, 15 who were still referred were tested by diagnostic ABR and 3 of them failed the test. On the other hand, gene screening identified 6 of the 865 newborns (0.7%) harbored homoplasmic 12S rRNA A1555G mutation although they passed the hearing screening.
  
  
  CONCLUSIONS
  It might be practical and effective to complement routine hearing screening in newborns with gene screening for the purpose of early diagnosis and discovery of the late-onset hearing loss.
authors.affiliation: Department of Otolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Guanming
authors.initials: G
authors.lastname: Chen
authors.forename: Xiyin
authors.initials: X
authors.lastname: Wang
authors.forename: Siqing
authors.initials: S
authors.lastname: Fu
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China; D015331:Cohort Studies; D004272:DNA, Mitochondrial / Q000235:genetics*; D016057:Evoked Potentials, Auditory, Brain Stem; D005188:False Negative Reactions; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D005820:Genetic Testing; D006312:Hearing Loss, Bilateral / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D006320:Hearing Tests / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009154:Mutation; D015997:Neonatal Screening / Q000379:methods*; D015995:Prevalence; D012335:RNA, Ribosomal / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018570:Risk Assessment; D012680:Sensitivity and Specificity
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S