title: Insufficient peroxiredoxin-2 expression in uterine NK cells obtained from a murine model of abortion.
abstract: The CBA/J × DBA/2 mouse mating combination is prone to spontaneous embryo loss, in contrast to the MHC-identical CBA/J × BALB/c mating combination, which yields successful pregnancies. The underlying mechanisms for these observations are unclear. In this study, multi-vision immunohistochemical staining (IHC), flow cytometry and Western blot analysis were used to detect peroxiredoxin-2 (PRX-2) expression in the uterine natural killer (uNK) cells from CBA/J × DBA/2 and CBA/J × BALB/c mice. In IHC analysis, co-localization of PRX-2 and lectin from Dolichos biflorus agglutinin (DBA-lectin) was confirmed and the frequency of PRX-2(+) DBA-lectin(+) cells was significantly lower in CBA/J × DBA/2 than CBA/J × BALB/c. In flow cytometry and Western blotting, PRX-2 was found expressed at a significantly lower level in CBA/J × DBA/2 mice. PRX-2 inhibition with a neutralizing antibody significantly decreased PRX-2 expression, increased the cytotoxicity of uNK cells, and increased the percentage of embryo loss in CBA/J × DBA/2J mice. Our data suggest that PRX-2 may be involved in the modulation of maternal-fetal tolerance and that insufficient expression of this protein may correlate with increased embryo loss in CBA/J × DBA/2J mice.
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Yin
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Shan
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhong
authors.forename: Jingfang
authors.initials: J
authors.lastname: Di
authors.forename: Qide
authors.initials: Q
authors.lastname: Lin
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D020964:Embryo Loss; D005260:Female; D006824:Hybridization, Genetic; D007694:Killer Cells, Natural / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D054464:Peroxiredoxins / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000378:metabolism; D010920:Placenta / Q000473:pathology; D037121:Plant Lectins; D011247:Pregnancy; D014599:Uterus / Q000378:metabolism* / Q000473:pathology
chemical_list: D037121:Plant Lectins; C036554:dolichos biflorus agglutinin; D054464:Peroxiredoxins