title: Identification of miRNomes in human liver and hepatocellular carcinoma reveals miR-199a/b-3p as therapeutic target for hepatocellular carcinoma.
abstract: The full scale of human miRNome in specific cell or tissue, especially in cancers, remains to be determined. An in-depth analysis of miRNomes in human normal liver, hepatitis liver, and hepatocellular carcinoma (HCC) was carried out in this study. We found nine miRNAs accounted for ∼88.2% of the miRNome in human liver. The third most highly expressed miR-199a/b-3p is consistently decreased in HCC, and its decrement significantly correlates with poor survival of HCC patients. Moreover, miR-199a/b-3p can target tumor-promoting PAK4 to suppress HCC growth through inhibiting PAK4/Raf/MEK/ERK pathway both in vitro and in vivo. Our study provides miRNomes of human liver and HCC and contributes to better understanding of the important deregulated miRNAs in HCC and liver diseases.
authors.affiliation: National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhou
authors.forename: Zhengxin
authors.initials: Z
authors.lastname: Wang
authors.forename: Guoshan
authors.initials: G
authors.lastname: Ding
authors.forename: Qiongzhu
authors.initials: Q
authors.lastname: Dong
authors.forename: Lunxiu
authors.initials: L
authors.lastname: Qin
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shi-Mei
authors.initials: SM
authors.lastname: Zhuang
authors.forename: Limin
authors.initials: L
authors.lastname: Zheng
authors.forename: Anmin
authors.initials: A
authors.lastname: Liang
authors.forename: Wenzhao
authors.initials: W
authors.lastname: Tao
authors.forename: Xuetao
authors.initials: X
authors.lastname: Cao
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D019175:DNA Methylation; D006509:Hepatitis B / Q000235:genetics; D006526:Hepatitis C / Q000235:genetics; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D035683:MicroRNAs