title: Monitoring of the h275y mutation in pandemic influenza A(H1N1) 2009 strains isolated in Malaysia.
abstract: Abstract. The 2009 pandemic influenza A(H1N1) infection in Malaysia was first reported in May 2009 and oseltamivir was advocated for confirmed cases in postexposure prophylaxis. However, there are cases of oseltamivir-resistance reported among H1N1-positive patients in other countries. Resistance is due to substitution of histidine by tyrosine at residue 275 (H275Y) of neuraminidase (NA). In this study, we have employed Sanger sequencing method to investigate the occurrence of mutations in NA segments of 67 pandemic 2009 A(H1N1) viral isolates from Malaysian patients that could lead to probable oseltamivir resistance. The sequencing analysis did not yield mutation at residue 275 for all 67 isolates indicating that our viral isolates belong to the wild type and do not confer resistance to oseltamivir.
authors.affiliation: Virology Unit, Institute for Medical Research, Kuala Lumpur, Malaysia. jeyanthi@imr.gov.my
authors.forename: Jeyanthi
authors.initials: J
authors.lastname: Suppiah
authors.forename: Mohd Apandi
authors.initials: MA
authors.lastname: Yusof
authors.forename: Khairul Azuan
authors.initials: KA
authors.lastname: Othman
authors.forename: T S
authors.initials: TS
authors.lastname: Saraswathy
authors.forename: Ravindran
authors.initials: R
authors.lastname: Thayan
authors.forename: Fauziah M
authors.initials: FM
authors.lastname: Kasim
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Murad
authors.forename: Zainah
authors.initials: Z
authors.lastname: Saat
mesh_terms: D019943:Amino Acid Substitution; D000998:Antiviral Agents / Q000627:therapeutic use; D004196:Disease Outbreaks; D024882:Drug Resistance, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000201:enzymology / Q000235:genetics*; D007251:Influenza, Human / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology*; D008296:Malaysia / Q000453:epidemiology; D020125:Mutation, Missense; D009439:Neuraminidase / Q000235:genetics*; D053139:Oseltamivir / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins; D053139:Oseltamivir; D009439:Neuraminidase