title: Expression and characterization of two new alkane-inducible cytochrome P450s from Trichoderma harzianum.
abstract: n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the ω-hydroxylase of lauric acid, which is a marker for ω-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids.
authors.affiliation: Institute of Clinical Physiology, National Research Council (CNR), via Moruzzi 1, Pisa, Italy. rdc@ifc.cnr.it
authors.forename: R
authors.initials: R
authors.lastname: Del Carratore
authors.forename: R Del
authors.initials: RD
authors.lastname: Carratore
authors.forename: P G
authors.initials: PG
authors.lastname: Gervasi
authors.forename: M P
authors.initials: MP
authors.lastname: Contini
authors.forename: P
authors.initials: P
authors.lastname: Beffy
authors.forename: B E
authors.initials: BE
authors.lastname: Maserti
authors.forename: G
authors.initials: G
authors.lastname: Giovannetti
authors.forename: A
authors.initials: A
authors.lastname: Brondolo
authors.forename: V
authors.initials: V
authors.lastname: Longo
mesh_terms: D000473:Alkanes / Q000378:metabolism / Q000494:pharmacology; D000595:Amino Acid Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D005800:Genes, Fungal; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics*
chemical_list: D000473:Alkanes; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System