title: Atg22p, a vacuolar membrane protein involved in the amino acid compartmentalization of Schizosaccharomyces pombe.
abstract: The fission yeast Schizosaccharomyces pombe has a homolog of the budding yeast Atg22p, which is involved in spore formation (Mukaiyama H. et al., Microbiology, 155, 3816-3826 (2009)). GFP-tagged Atg22p in the fission yeast was localized to the vacuolar membrane. Upon disruption of atg22, the amino acid levels of the cellular fraction as well as the vacuolar fraction decreased. The uptake of several amino acids, such as lysine, histidine, and arginine, was impaired in atg22Δ cells. S. pombe Atg22p plays an important role in the compartmentalization of amino acids.
authors.affiliation: Department of Applied Bioresource Science, Faculty of Agriculture, Ehime University, Matsuyama, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Tomoko
authors.initials: T
authors.lastname: Iwaki
authors.forename: Soracom
authors.initials: S
authors.lastname: Chardwiriyapreecha
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Shimazu
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000071183:Autophagy-Related Proteins; D001692:Biological Transport; D008565:Membrane Proteins / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D000596:Amino Acids; C558594:Atg22 protein, S pombe; D000071183:Autophagy-Related Proteins; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins