title: Efficient production of D-glucosaminic acid from D-glucosamine by Pseudomonas putida GNA5.
abstract: D-glucosaminic acid was produced efficiently from glucosamine by oxidative fermentation using a newly isolated strain, Pseudomonas putida GNA5. After optimization of the fermentation process, 51.5 g L(-1) D-glucosaminic acid was produced from an initial concentration of 60 g L(-1) D-glucosamine-HCl after 72 h of oxidative fermentation, which corresponded to a molar yield of 95.4%. This production process is potentially of considerable economic significance because very few by-products were detected. Furthermore, D-glucosaminic acid was accumulated stably during the oxidative fermentation process without the addition of an inhibitor of D-glucosaminic acid breakdown, even though D-glucosamine was exhausted. These results suggest that the mechanisms of D-glucosaminic acid-related metabolism differ between Pseudomonas putida GNA5 and the strain Pseudomonas genera, which was previously reported to produce D-glucosaminic acid.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, Jiangsu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Zhongzhong
authors.initials: Z
authors.lastname: Bai
authors.forename: Xiao
authors.initials: X
authors.lastname: Meng
authors.forename: Bingfang
authors.initials: B
authors.lastname: He
mesh_terms: D005285:Fermentation; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000378:metabolism*
chemical_list: C035547:glucosaminic acid; D005944:Glucosamine