title: Combined measurement of PEDF, haptoglobin and tau in cerebrospinal fluid improves the diagnostic discrimination between alzheimer's disease and other dementias.
abstract: Using proteomic approach in cerebrospinal fluid (CSF) we identified pigment epithelium-derived factor (PEDF) and Haptoglobin (Hp) as putative markers that could discriminate between AD and other dementias. ELISA assays were developed to measure the levels of PEDF and Hp in CSF from patients with AD (AD, n=27), non-AD (NAD, n=30) and in non-demented patients (ND, n=27). The combined assessment of PEDF, Hp and Tau levels, using Iterative Marginal Optimization, improved the differential diagnosis of AD, especially in patients with moderate to severe dementia (p<0.002). This pilot study highlights the probable different contribution of oxidative mechanisms in dementia.
authors.affiliation: SysDiag CNRS / Bio-Rad UMR3145, 1682 rue de la Valsiere, Montpellier, France. jean-daniel.abraham@sysdiag.cnrs.fr
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Abraham
authors.forename: Sophie
authors.initials: S
authors.lastname: Calvayrac-Pawlowski
authors.forename: Sandra
authors.initials: S
authors.lastname: Cobo
authors.forename: Nicolas
authors.initials: N
authors.lastname: Salvetat
authors.forename: Guillaume
authors.initials: G
authors.lastname: Vicat
authors.forename: Laurence
authors.initials: L
authors.lastname: Molina
authors.forename: Jacques
authors.initials: J
authors.lastname: Touchon
authors.forename: Bernard-François
authors.initials: BF
authors.lastname: Michel
authors.forename: Franck
authors.initials: F
authors.lastname: Molina
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Verdier
authors.forename: Jeannette
authors.initials: J
authors.lastname: Fareh
authors.forename: Chantal
authors.initials: C
authors.lastname: Mourton-Gilles
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D000595:Amino Acid Sequence; D000906:Antibodies / Q000378:metabolism; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D015140:Dementia, Vascular / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000134:cerebrospinal fluid*; D005260:Female; D057180:Frontotemporal Dementia / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006242:Haptoglobins / Q000134:cerebrospinal fluid*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000134:cerebrospinal fluid*; D010084:Oxidation-Reduction; D010865:Pilot Projects; D040901:Proteomics; D015843:Serpins / Q000134:cerebrospinal fluid*; D012720:Severity of Illness Index; D016875:tau Proteins / Q000134:cerebrospinal fluid*; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D000906:Antibodies; D015415:Biomarkers; D005136:Eye Proteins; D006242:Haptoglobins; D009414:Nerve Growth Factors; D015843:Serpins; D016875:tau Proteins; D000099174:Pigment Epithelium-Derived Factor