title: NTPDase activity in human lymphocytes is not affected by therapeutic doses of anti-HIV drugs.
abstract: NTPDase (EC 3.6.1.5) is an enzyme that hydrolyzes extracellular nucleoside tri- and/or diphoshates forming AMP that can serve as a substrate for an ecto-5'-nucleotidase (EC 3.1.3.5) with liberation of adenosine, a modulator of vascular tone and inhibitor of platelet aggregation. These enzymes also occur in lymphocytes playing an important role in immune function. In this study, it was investigated if anti-HIV therapy could affect NTPDase activity in human lymphocytes. Samples of lymphocytes were incubated with different concentrations of anti-HIV drugs and NTPDase activity was determined by colorimetric assay with quantification of inorganic phosphate released. There is not significant difference of NTPDase activity among samples with therapeutic doses of anti-HIV drugs tested when compared with controls. NTPDase activity in peripheral human lymphocytes is not altered by anti-HIV therapy.
authors.affiliation: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Avenida Roraima, 1000, prédio 20, 97105-900, Santa Maria, RS, Brazil. danibrl@smail.ufsm.br
authors.forename: Daniela B R
authors.initials: DB
authors.lastname: Leal
authors.forename: Maria R C
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Claudio A M
authors.initials: CA
authors.lastname: Leal
authors.forename: Claudia de M
authors.initials: Cde M
authors.lastname: Bertoncheli
authors.forename: Vera M
authors.initials: VM
authors.lastname: Morsch
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D008297:Male
chemical_list: D019380:Anti-HIV Agents; D015703:Antigens, CD; D001081:Apyrase; C067869:CD39 antigen