title: Fast and sensitive LC-DAD-ESI/MS method for analysis of saikosaponins c, a, and d from the roots of Bupleurum Falcatum (Sandaochaihu).
abstract: In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 x 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
authors.affiliation: The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College, Nanning 530011, China.
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: He
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Li
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.forename: Wen-Cong
authors.initials: WC
authors.lastname: Liu
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Liang
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D