title: Improvement of blood compatibility on polysulfone-polyvinylpyrrolidone blend films as a model membrane of dialyzer by physical adsorption of recombinant soluble human thrombomodulin (ART-123).
abstract: ART-123 is a recombinant soluble human thrombomodulin (hTM) with potent anticoagulant activity, and is available for developing antithrombogenic surfaces by immobilization. We focused on improving blood compatibility on the dialyzer surface by the physical adsorption of ART-123 as a  safe yet simple method without using chemical reagents. The physical adsorption mechanism and anticoagulant activities of adsorbed hTM on the surface of a polysulfone (PSF) membrane containing polyvinylpyrrolidone (PVP) as a model dialyzer were investigated in detail. The PVP content of the  PSF-PVP films was saturated at 20 wt% after immersion in Tris-HCl buffer, even with the addition of over 20 wt% PVP. The surface morphology of the PSF-PVP films was strongly influenced by the PVP content, because PVP covered the outermost surface of the PSF-PVP films. The  adsorption speed of hTM slowed dramatically with increasing PVP content up to 10 wt%, but the maximum adsorption amount of hTM onto the PSF-PVP film surface was almost the same, regardless of the PVP content. The PSF-PVP film with the physically adsorbed hTM showed higher protein  C activity as compared to the PSF film, it showed excellent blood compatibility due to the protein C activity and the inhibition properties of platelet adhesion. The physical adsorption of hTM can be useful as a safe yet simple method to improve the blood compatibility of a dialyzer surface.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Omichi
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsusaki
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
mesh_terms: D000327:Adsorption; D000925:Anticoagulants / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects; D011108:Polymers / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry*; D011486:Protein C / Q000378:metabolism; D011994:Recombinant Proteins / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones / Q000737:chemistry*; D013499:Surface Properties; D018180:Thrombomodulin / Q000737:chemistry*; D013997:Time Factors
chemical_list: C472045:ART123; D000925:Anticoagulants; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D011486:Protein C; D011994:Recombinant Proteins; D013450:Sulfones; D018180:Thrombomodulin; C017662:polysulfone P 1700; D011205:Povidone