title: Population genetic analysis and phylogeny reconstruction in Eucalyptus (Myrtaceae) using high-throughput, genome-wide genotyping.
abstract: A set of over 8000 Diversity Arrays Technology (DArT) markers was tested for its utility in high-resolution population and phylogenetic studies across a range of Eucalyptus taxa. Small-scale population studies of Eucalyptus camaldulensis, Eucalyptus cladocalyx, Eucalyptus globulus, Eucalyptus grandis, Eucalyptus nitens, Eucalyptus pilularis and Eucalyptus urophylla demonstrated the potential of genome-wide genotyping with DArT markers to differentiate species, to identify interspecific hybrids and to resolve biogeographic disjunctions within species. The population genetic studies resolved geographically partitioned clusters in E. camaldulensis, E. cladocalyx, E. globulus and E. urophylla that were congruent with previous molecular studies. A phylogenetic study of 94 eucalypt species provided results that were largely congruent with traditional taxonomy and ITS-based phylogenies, but provided more resolution within major clades than had been obtained previously. Ascertainment bias (the bias introduced in a phylogeny from using markers developed in a small sample of the taxa that are being studied) was not detected. DArT offers an unprecedented level of resolution for population genetic, phylogenetic and evolutionary studies across the full range of Eucalyptus species.
authors.affiliation: School of Plant Science, University of Tasmania, Private Bag 55, Hobart, Tasmania 7001, Australia. Dorothy.Steane@utas.edu.au
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Steane
authors.forename: Dean
authors.initials: D
authors.lastname: Nicolle
authors.forename: Carolina P
authors.initials: CP
authors.lastname: Sansaloni
authors.forename: César D
authors.initials: CD
authors.lastname: Petroli
authors.forename: Jason
authors.initials: J
authors.lastname: Carling
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kilian
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Myburg
authors.forename: Dario
authors.initials: D
authors.lastname: Grattapaglia
authors.forename: René E
authors.initials: RE
authors.lastname: Vaillancourt
mesh_terms: D001499:Bayes Theorem; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005052:Eucalyptus / Q000145:classification / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D055106:Genome-Wide Association Study / Q000379:methods*; D005838:Genotype; D057166:High-Throughput Screening Assays / Q000379:methods; D006824:Hybridization, Genetic; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010802:Phylogeny; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results
chemical_list: D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers