title: Mapping heat exchange in an allosteric protein.
abstract: Nicotinic acetylcholine receptors (AChRs) are synaptic ion channels that spontaneously isomerize (i.e., gate) between resting and active conformations. We used single-molecule electrophysiology to measure the temperature dependencies of mouse neuromuscular AChR gating rate and equilibrium constants. From these we estimated free energy, enthalpy, and entropy changes caused by mutations of amino acids located between the transmitter binding sites and the middle of the membrane domain. The range of equilibrium enthalpy change (13.4 kcal/mol) was larger than for free energy change (5.5 kcal/mol at 25°C). For two residues, the slope of the rate-equilibrium free energy relationship (Φ) was approximately constant with temperature. Mutant cycle analysis showed that both free energies and enthalpies are additive for energetically independent mutations. We hypothesize that changes in energy associated with changes in structure mainly occur close to the site of the mutation, and, hence, that it is possible to make a residue-by-residue map of heat exchange in the AChR gating isomerization. The structural correlates of enthalpy changes are discussed for 12 different mutations in the protein.
authors.affiliation: Department of Physiology and Biophysics, State University of New York, Buffalo, New York, USA.
authors.forename: Shaweta
authors.initials: S
authors.lastname: Gupta
authors.forename: Anthony
authors.initials: A
authors.lastname: Auerbach
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D001665:Binding Sites; D057809:HEK293 Cells; D006358:Hot Temperature; D006801:Humans; D015640:Ion Channel Gating; D007700:Kinetics; D051379:Mice; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013696:Temperature
chemical_list: D050505:Mutant Proteins; D011978:Receptors, Nicotinic