title: Isolation of bacterial ribosomes with monolith chromatography.
abstract: We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology.
authors.affiliation: Department of Life Sciences, Imperial College London, London, United Kingdom.
authors.forename: Andrej
authors.initials: A
authors.lastname: Trauner
authors.forename: Mark H
authors.initials: MH
authors.lastname: Bennett
authors.forename: Huw D
authors.initials: HD
authors.lastname: Williams
mesh_terms: D000837:Anion Exchange Resins / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D020102:Mycobacterium smegmatis / Q000737:chemistry; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism; D013395:Sucrose / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000837:Anion Exchange Resins; D020543:Proteome; D013395:Sucrose