title: Illuminating transcription pathways using fluorescent reporter genes and yeast functional genomics.
abstract: Technological advances have enabled researchers to probe gene regulatory pathways on an unprecedented scale. Here, we summarize our recent work that exploits a systematic screening approach in the budding yeast to discover regulators of a promoter of interest. We discuss future applications of our approach based on emerging themes in the literature.
authors.affiliation: University of Toronto, ON Canada.
authors.forename: Pinay
authors.initials: P
authors.lastname: Kainth
authors.forename: Brenda
authors.initials: B
authors.lastname: Andrews
mesh_terms: D015966:Gene Expression Regulation, Fungal; D053263:Gene Regulatory Networks; D017930:Genes, Reporter / Q000235:genetics; D023281:Genomics / Q000379:methods*; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D008164:Luminescent Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors