title: Predictive value of TP53 fluorescence in situ hybridization in cytogenetic subgroups of acute myeloid leukemia.
abstract: Acute myeloid leukemia (AML) with a complex karyotype (CK) has frequent alterations in TP53 and a very poor prognosis. We examined whether a prompt and simple fluorescence in situ hybridization (FISH) analysis for 17p13 deletion at diagnosis has a predictive value for response to therapy and overall survival in subgroups of AML. In 15 patients with a normal karyotype the TP53 FISH analysis was normal, whereas in 16 patients with CK 75% had only one copy of the TP53 allele. The deletion was also detected in 33% of six patients with monosomy or partial monosomy of chromosome 5, 7, 9, or 12. This loss of TP53 correlated significantly with a poor response to chemotherapy, and the median survival time of these patients was shorter. TP53 FISH analysis carried out at diagnosis has a predictive value with respect to chemotherapy response and can therefore facilitate a rapid decision on treatment strategies.
authors.affiliation: Department of Hematology and Bone Marrow Transplantation, Tel-Aviv Sourasky Medical Center, Israel. tavorsigal@yahoo.com
authors.forename: Sigal
authors.initials: S
authors.lastname: Tavor
authors.forename: Rachel
authors.initials: R
authors.lastname: Rothman
authors.forename: Tamar
authors.initials: T
authors.lastname: Golan
authors.forename: Nadia
authors.initials: N
authors.lastname: Voskoboinik
authors.forename: Ben-Zion
authors.initials: BZ
authors.lastname: Katz
authors.forename: Nadav
authors.initials: N
authors.lastname: Sarid
authors.forename: Ruth
authors.initials: R
authors.lastname: Shomrat
authors.forename: Avi
authors.initials: A
authors.lastname: Orr-Urtreger
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Naparstek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use; D005260:Female; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D016019:Survival Analysis; D016896:Treatment Outcome; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53