title: [Current approaches to laboratory diagnosis of syphilis].
abstract: The efficiency of chemiluminescence immunoassay (CIA) used as a treponemal test in the serological diagnosis of syphilis was evaluated, by applying the serum samples of 219 patients screened for syphilitic infection. The efficiency of CIA was evaluated in two steps: (1) comparison of the results of tests using the IMMULITE 2000 Syphilis Screen and LIAISON Syphilis screen reagent kits; (2) that of the results of tests applying the IMMULITE 2000 Syphilis Screen and Recombi Best antipallidum-summary antibodies kits. A 100% correlation was found when comparing the results of tests employing the IMMULITE 2000 Syphilis Screen and DiaSorin LIAISON Syphilis Screen kits (n=66). Comparison of CIA using the IMMULITE 2000 Syphilis Screen kits versus that applying the Recombi Best antipallidum-summary antibodies kits (n=153) revealed 3 discordant samples, which amounted to 1.96% of the number of results. Evaluation of the impact of related microorganisms on the detection of T. pallidum antibodies (n= 140) with the IMMULITE 2000 Syphilis Screen kit indicated no interference or cross-reactivity.
authors.forename: V P
authors.initials: VP
authors.lastname: Bondareva
authors.forename: V P
authors.initials: VP
authors.lastname: Deriabina
authors.forename: E N
authors.initials: EN
authors.lastname: Zakharova
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Kotel'nikova
authors.forename: T A
authors.initials: TA
authors.lastname: Osipova
authors.forename: N M
authors.initials: NM
authors.lastname: Pugacheva
authors.forename: A V
authors.initials: AV
authors.lastname: Ruzhanskaia
authors.forename: O A
authors.initials: OA
authors.lastname: Poletaeva
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D001322:Autoanalysis; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D013587:Syphilis / Q000175:diagnosis*; D013588:Syphilis Serodiagnosis / Q000379:methods; D014210:Treponema pallidum / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial