title: Neural crest-derived multipotent cells in the adult mouse iris stroma.
abstract: The purpose of this study was to characterize neural crest-derived cells within the adult murine iris. The iris was isolated from P0-Cre/Floxed-EGFP transgenic (TG) mice. The isolated iris cells formed EGFP-positive spheres on non-adhesive culture plates. Immunostaining showed that these EGFP-positive spheres expressed neural crest markers including Sox10 and p75NTR, and these cells showing in vitro sphere-forming ability were originally resided in the iris stroma (IS), in vivo. Real-time RT-PCR showed that the EGFP-positive spheres expressed significantly higher levels of the neural crest markers than EGFP-negative spheres and bone marrow-derived mesenchymal stem cells. Furthermore, the iris stromal sphere had capability to differentiate into various cell lineages including smooth muscle and cartilage. These data indicate that neural crest-derived multipotent cells can be isolated from the murine IS and expanded in sphere culture.
authors.affiliation: Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Hayashi
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kanakubo
authors.forename: Ayumi
authors.initials: A
authors.lastname: Ogasawara
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Noriko
authors.initials: N
authors.lastname: Osumi
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishida
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques; D019070:Cell Lineage; D049452:Green Fluorescent Proteins / Q000235:genetics; D007498:Iris / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology*; D009432:Neural Crest / Q000166:cytology*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins