title: Phosphocreatine preconditioning attenuates apoptosis in ischemia-reperfusion injury of rat brain.
abstract: Phosphocreatine (PCr) is an endogenous compound containing high-energy phosphate bonds. It has been confirmed that PCr is effective in preventing and treating cardiac and renal ischemia-reperfusion injury. In this study, rat cerebral ischemia-reperfusion injury models were constructed. Apoptotic cells in the cortex region were measured by TUNEL method. Malondialdehyde (MDA) content was detected by chromatometry, and calmodulin (CaM) activity was detected by ELISA. Compared with sham-operated group (sham group), TUNEL-positive cells, MDA, and level of CaM activity increased in ischemia-reperfusion group (I/R group) and PCr preconditioning group (PCr group); compared with I/R group, TUNEL-positive cells, MDA content, and level of CaM activity decreased in PCr group. This study indicated that PCr can decrease the morphological damage and the neuron apoptosis of the ischemia-reperfusion injury brain through attenuating abnormalities of calcium balance and production of oxygen free radicals.
authors.affiliation: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Ling-Hua
authors.initials: LH
authors.lastname: Tang
authors.forename: Zhong-Yuan
authors.initials: ZY
authors.lastname: Xia
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Wei
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.forename: Qing-Tao
authors.initials: QT
authors.lastname: Meng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology*; D002147:Calmodulin / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D020287:In Situ Nick-End Labeling; D019194:Ischemic Preconditioning; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010725:Phosphocreatine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*
chemical_list: D002147:Calmodulin; D010725:Phosphocreatine; D008315:Malondialdehyde