title: Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum.
abstract: We demonstrate glutamate production from β-glucan using endoglucanase (EG)-expressing Corynebacterium glutamicum. The signal sequence torA derived from Escherichia coli K12, which belongs to the Tat pathway, was suitable for secreting EG of Clostridium thermocellum using C. glutamicum as a host. Using the torA signal sequence, endoglucanase from Clostridium cellulovorans 743B was successfully expressed, and the secreted EG produced 123 mg of reducing sugar from 5 g of β-glucan at 30 °C for 72 h, which is the optimal condition for C. glutamicum growth. Subsequently, glutamate fermentation from β-glucan was carried out with the addition of Aspergillus aculeatus β-glucosidase produced by recombinant Aspergillus oryzae. Using EG-secreting C. glutamicum, 178 mg/l of glutamate was produced from 15 g of β-glucan. This is the first report of glutamate fermentation from β-glucan using endoglucanase-secreting C. glutamicum.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Tsuchidate
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Tateno
authors.forename: Naoko
authors.initials: N
authors.lastname: Okai
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D048013:Acetivibrio thermocellus / Q000201:enzymology / Q000235:genetics; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D018698:Glutamic Acid / Q000378:metabolism*; D015202:Protein Engineering; D021381:Protein Transport; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D047071:beta-Glucans; D018698:Glutamic Acid; D002480:Cellulase