title: Bisubstrate specificity in histidine/tryptophan biosynthesis isomerase from Mycobacterium tuberculosis by active site metamorphosis.
abstract: In histidine and tryptophan biosynthesis, two related isomerization reactions are generally catalyzed by two specific single-substrate enzymes (HisA and TrpF), sharing a similar (β/α)(8)-barrel scaffold. However, in some actinobacteria, one of the two encoding genes (trpF) is missing and the two reactions are instead catalyzed by one bisubstrate enzyme (PriA). To unravel the unknown mechanism of bisubstrate specificity, we used the Mycobacterium tuberculosis PriA enzyme as a model. Comparative structural analysis of the active site of the enzyme showed that PriA undergoes a reaction-specific and substrate-induced metamorphosis of the active site architecture, demonstrating its unique ability to essentially form two different substrate-specific actives sites. Furthermore, we found that one of the two catalytic residues in PriA, which are identical in both isomerization reactions, is recruited by a substrate-dependent mechanism into the active site to allow its involvement in catalysis. Comparison of the structural data from PriA with one of the two single-substrate enzymes (TrpF) revealed substantial differences in the active site architecture, suggesting independent evolution. To support these observations, we identified six small molecule compounds that inhibited both PriA-catalyzed isomerization reactions but had no effect on TrpF activity. Our data demonstrate an opportunity for organism-specific inhibition of enzymatic catalysis by taking advantage of the distinct ability for bisubstrate catalysis in the M. tuberculosis enzyme.
authors.affiliation: European Molecular Biology Laboratory, Hamburg Unit, Notkestrasse 85, D-22603 Hamburg, Germany.
authors.forename: Anne V
authors.initials: AV
authors.lastname: Due
authors.forename: Jochen
authors.initials: J
authors.lastname: Kuper
authors.forename: Arie
authors.initials: A
authors.lastname: Geerlof
authors.forename: Jens Peter
authors.initials: JP
authors.lastname: von Kries
authors.forename: Matthias
authors.initials: M
authors.lastname: Wilmanns
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis / Q000187:drug effects; D020134:Catalytic Domain; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006639:Histidine / Q000096:biosynthesis*; D007535:Isomerases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D007536:Isomerism; D008024:Ligands; D008958:Models, Molecular; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology*; D017433:Protein Structure, Secondary; D013379:Substrate Specificity / Q000187:drug effects; D014364:Tryptophan / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D008024:Ligands; D006639:Histidine; D014364:Tryptophan; D007535:Isomerases