title: Evaluation of recombinant Lig antigen-based ELISA for detection of leptospiral antibodies in canine sera.
abstract: Abstract. The objectives of this study were to clone the conserved region of leptospiral immunoglobulin-like protein (lig) gene and evaluate the utility of the recombinant Lig as an ELISA antigen for detection of leptospiral antibodies in canine sera. Leptospira kirschneri serovar Grippotyposa strain Moskva V was chosen to be a target for cloning the conserved region of Lig gene. This assay was evaluated with canine sera (n = 91) that were MAT-negative (< 1:100 dilution) and sera (n = 103) that were MAT-positive (> or = 1:100 dilution) using 24 serovars. The ELISA showed a relative sensitivity as compared to MAT of 84.5% whereas the specificity was 76.9%. This assay is simple and can be routinely prepared in large amounts. It was concluded that the GST.Lig recombinant protein-based ELISA could be used as a screening test for serodiagnosis of canine leptospirosis with also for confirmation of MAT-positive test results.
authors.affiliation: Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom.
authors.forename: Anchalee
authors.initials: A
authors.lastname: La-Ard
authors.forename: Patamaporn
authors.initials: P
authors.lastname: Amavisit
authors.forename: Thavajchai
authors.initials: T
authors.lastname: Sukpuaram
authors.forename: Worawidh
authors.initials: W
authors.lastname: Wajjwalku
mesh_terms: D000372:Agglutination Tests / Q000379:methods; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood / Q000302:isolation & purification; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D007136:Immunoglobulins / Q000032:analysis / Q000097:blood / Q000276:immunology; D007919:Leptospira / Q000276:immunology* / Q000302:isolation & purification*; D007922:Leptospirosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013785:Thailand
chemical_list: D000907:Antibodies, Bacterial; D007136:Immunoglobulins