title: Short communication: decrease in mitochondrial transmembrane potential in peripheral blood mononuclear cells of HIV-uninfected subjects undergoing HIV postexposure prophylaxis.
abstract: To monitor the mitochondrial transmembrane potential (ΔΨm), peripheral blood mononuclear cells (PBMCs) from 18 HIV-uninfected individuals completing a 4-week course of HIV postexposure prophylaxis (PEP) with emtricitabine/tenofovir and lopinavir/ritonavir were analyzed. ΔΨm was assessed by FACS analysis after staining with JC-1 using a mixed effects regression analysis. Apoptosis was determined by Annexin-V-FITC and propidium iodide staining. ΔΨm decreased significantly during HIV-PEP (p=0.0015 for the linear term, p=0.0039 for the quadratic term of days on PEP) and normalized several weeks after stopping antiretrovirals. The apoptosis rates did not change significantly (p=0.42). Even newer drugs such as tenofovir and emtricitabine may cause side effects due to mitochondrial dysfunction.
authors.affiliation: Division of Infectious Diseases, Medizinische Poliklinik-Innenstadt, University of Munich, Germany.
authors.forename: Jan B
authors.initials: JB
authors.lastname: Groener
authors.forename: Ulrich
authors.initials: U
authors.lastname: Seybold
authors.forename: Thomas
authors.initials: T
authors.lastname: Vollbrecht
authors.forename: Johannes R
authors.initials: JR
authors.lastname: Bogner
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000009:adverse effects*; D017209:Apoptosis; D018890:Chemoprevention / Q000009:adverse effects / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D015658:HIV Infections / Q000517:prevention & control*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008564:Membrane Potentials; D008875:Middle Aged; D051336:Mitochondrial Membranes / Q000502:physiology*; D056990:Post-Exposure Prophylaxis / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D019380:Anti-HIV Agents