title: In situ biodiesel production from fast-growing and high oil content Chlorella pyrenoidosa in rice straw hydrolysate.
abstract: Rice straw hydrolysate was used as lignocellulose-based carbon source for Chlorella pyrenoidosa cultivation and the feasibility of in situ biodiesel production was investigated. 13.7 g/L sugar was obtained by enzymatic hydrolyzation of rice straw. Chlorella pyrenoidosa showed a rapid growth in the rice straw hydrolysate medium, the maximum biomass concentration of 2.83 g/L was obtained in only 48 hours. The lipid content of the cells reached as high as 56.3%. In situ transesterification was performed for biodiesel production. The optimized condition was 1 g algal powder, 6 mL n-hexane, and 4 mL methanol with 0.5 M sulfuric acid at the temperature of 90°C in 2-hour reaction time, under which over 99% methyl ester content and about 95% biodiesel yield were obtained. The results suggested that the method has great potential in the production of biofuels with lignocellulose as an alternative carbon source for microalgae cultivation.
authors.affiliation: Key Laboratory of MOE for Microbial Metabolism and School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Penglin
authors.initials: P
authors.lastname: Li
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Miao
authors.forename: Rongxiu
authors.initials: R
authors.lastname: Li
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Zhong
mesh_terms: D056804:Biofuels / Q000032:analysis*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism; D002708:Chlorella / Q000166:cytology / Q000254:growth & development*; D002725:Chloroform / Q000494:pharmacology; D004951:Esterification / Q000187:drug effects; D006586:Hexanes / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D000432:Methanol / Q000494:pharmacology; D012275:Oryza / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D012997:Solvents / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014866:Waste Products / Q000032:analysis*
chemical_list: D056804:Biofuels; D006586:Hexanes; D010938:Plant Oils; D012997:Solvents; D014866:Waste Products; C026385:n-hexane; D002244:Carbon; D002725:Chloroform; D002480:Cellulase; D000432:Methanol