title: In vitro manipulation of endothelial progenitor cell adhesion to vascular endothelium and extracellular matrix by the phorbol ester PMA.
abstract: Injection of endothelial progenitor cells (EPCs) into arteries for cell therapy is a promising field in regenerative medicine. However, adhesion of EPCs during capillary passage is restricted, and non-adhering cells are lost into circulation. Here we demonstrate that it is possible to achieve a three- to sevenfold higher rate of EPC adhesion to endothelium and extracellular matrix molecules after short-term activation with phorbol myristate acetate (PMA). In addition, differentiation and toxicity analyses of PMA activated EPCs showed no impact on cell differentiation and negligible impact on cell survival.
authors.affiliation: Department of Trauma Surgery, Johann Wolfgang Goethe University, Frankfurt/Main, Germany. maciej-janusz.powerski@charite.de
authors.forename: Maciej
authors.initials: M
authors.lastname: Powerski
authors.forename: Dirk
authors.initials: D
authors.lastname: Henrich
authors.forename: Anna
authors.initials: A
authors.lastname: Sander
authors.forename: Anna
authors.initials: A
authors.lastname: Teiler
authors.forename: Ingo
authors.initials: I
authors.lastname: Marzi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology*; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007797:Laminin / Q000378:metabolism; D009336:Necrosis / Q000139:chemically induced; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D015415:Biomarkers; D005353:Fibronectins; D007797:Laminin; D003094:Collagen; D013755:Tetradecanoylphorbol Acetate