title: Carcinoembryonic antigen admittance biosensor based on Au and ZnO nanoparticles using FFT admittance voltammetry.
abstract: In this work, a highly sensitive carcinoembryonic antigen fast Fourier transform admittance biosensor is introduced. The proposed biosensor is based on bilayer films of ZnO/Au nanoparticles as an immobilization matrix. These layers are prepared by self-assembly and deposition method on a gold electrode surface, respectively. Carcinoembryonic antibody (anti-CEA) was immobilized on gold nanoparticles and positively charged horseradish peroxidase (HRP) was used to block sites against nonspecific binding. The admittance biosensor was developed based on fast Fourier transform continuous square wave voltammetry, which produces a sensitive, fast (less than 20 s) and reliable response for determination of carcinoembryonic antigen. The technique was applied as a detector in a flow injection system. The admittances reduction current of the biosensor decreases linearly in two concentrations ranges of CEA from 0.1 to 70 ng/mL and from 70 to 200 ng/mL with a detection limit of 0.01 ng/mL in presence of 0.5 mM H(2)O(2) as an eluent solution.
authors.affiliation: Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran. norouzi@khayam.ut.ac.ir
authors.forename: Parviz
authors.initials: P
authors.lastname: Norouzi
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Gupta
authors.forename: Farnoush
authors.initials: F
authors.lastname: Faridbod
authors.forename: Morteza
authors.initials: M
authors.lastname: Pirali-Hamedani
authors.forename: Bagher
authors.initials: B
authors.lastname: Larijani
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
mesh_terms: D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000737:chemistry*; D055664:Electrochemical Techniques; D005583:Fourier Analysis; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D002272:Carcinoembryonic Antigen; D006046:Gold; D006735:Horseradish Peroxidase; D015034:Zinc Oxide