title: Complete genome sequence of Rhizobium leguminosarum bv. trifolii strain WSM1325, an effective microsymbiont of annual Mediterranean clovers.
abstract: Rhizobium leguminosarum bv trifolii is a soil-inhabiting bacterium that has the capacity to be an effective nitrogen fixing microsymbiont of a diverse range of annual Trifolium (clover) species. Strain WSM1325 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from root nodules collected in 1993 from the Greek Island of Serifos. WSM1325 is produced commercially in Australia as an inoculant for a broad range of annual clovers of Mediterranean origin due to its superior attributes of saprophytic competence, nitrogen fixation and acid-tolerance. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a microsymbiont of annual clovers. We reveal that its genome size is 7,418,122 bp encoding 7,232 protein-coding genes and 61 RNA-only encoding genes. This multipartite genome contains 6 distinct replicons; a chromosome of size 4,767,043 bp and 5 plasmids of size 828,924 bp, 660,973 bp, 516,088 bp, 350,312 bp and 294,782 bp.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
authors.forename: Graham
authors.initials: G
authors.lastname: O'Hara
authors.forename: Patrick
authors.initials: P
authors.lastname: Chain
authors.forename: Julie
authors.initials: J
authors.lastname: Ardley
authors.forename: Lambert
authors.initials: L
authors.lastname: Bräu
authors.forename: Kemanthi
authors.initials: K
authors.lastname: Nandesena
authors.forename: Ravi
authors.initials: R
authors.lastname: Tiwari
authors.forename: Alex
authors.initials: A
authors.lastname: Copeland
authors.forename: Matt
authors.initials: M
authors.lastname: Nolan
authors.forename: Cliff
authors.initials: C
authors.lastname: Han
authors.forename: Thomas
authors.initials: T
authors.lastname: Brettin
authors.forename: Miriam
authors.initials: M
authors.lastname: Land
authors.forename: Galina
authors.initials: G
authors.lastname: Ovchinikova
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mavromatis
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.forename: Vanessa
authors.initials: V
authors.lastname: Melino
authors.forename: Matthew
authors.initials: M
authors.lastname: Denton
authors.forename: Ron
authors.initials: R
authors.lastname: Yates
authors.forename: John
authors.initials: J
authors.lastname: Howieson