title: Synthesis and photophysical properties of new SNARF derivatives as dual emission pH sensors.
abstract: We report the synthesis and properties of two new seminaphthorhodafluor (SNARF) derivatives, SNARF-F and SNARF-Cl. Both these derivatives exhibit typical red shifts of absorption and fluorescence, and higher cell permeability as compared to traditional SNARF, while the pH-dependent dual-emission characteristics are well retained. In particular, the lower pK(a) (7.38) of SNARF-F makes it more suitable than traditional SNARF derivatives for intracellular applications.
authors.affiliation: Department of Life System, Graduate School, The University of Tokushima, Tokushima, Japan. nakata@iae.kyoto-u.ac.jp
authors.forename: Eiji
authors.initials: E
authors.lastname: Nakata
authors.forename: Yoshijiro
authors.initials: Y
authors.lastname: Nazumi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yukimachi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Uto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maezawa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hori
mesh_terms: D001578:Benzopyrans / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010777:Photochemistry; D013050:Spectrometry, Fluorescence
chemical_list: D001578:Benzopyrans; D005456:Fluorescent Dyes; C063917:SNARF dye