title: Dysfunction of the fusion of pre-synaptic plasma membranes and synaptic vesicles caused by oxidative stress, and its prevention by vitamin E.
abstract: To define whether hyperoxia induces the dysfunction of membrane fusion between synaptic vesicles with pre-synaptic plasma membranes in the nerve terminals, and whether vitamin E prevents this abnormal event, we investigated the influence of hyperoxia on the fusion ability of isolated synaptic vesicles and the inside-out type pre-synaptic plasma membrane vesicles from rat brain using the fluorescence tracing method. The membrane fusion ability of both membranes from rats subjected to hyperoxia was markedly decreased compared with the membranes from a normal rat. Rats subjected to hyperoxia in the form of oxidative stress showed significant increases in the levels of thiobarbituric acid reactive substances (TBARS), conjugated dienes, and protein carbonyl moieties in both synaptic vesicles and pre-synaptic plasma membranes. When rats were supplemented with vitamin E, these abnormalities were inhibited even when rats were subjected to hyperoxia.
authors.affiliation: Division of Biochemistry, Department of Bioscience & Engineering, Shibaura Institute of Technology, Saitama, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Arai
authors.forename: Mitsunari
authors.initials: M
authors.lastname: Saito
authors.forename: Hirokatsu
authors.initials: H
authors.lastname: Takatsu
authors.forename: Koji
authors.initials: K
authors.lastname: Fukui
authors.forename: Shiro
authors.initials: S
authors.lastname: Urano
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D008297:Male; D008561:Membrane Fusion / Q000187:drug effects / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013572:Synaptic Vesicles / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014810:Vitamin E