title: Inhibitory effects of chelidonic acid on IL-6 production by blocking NF-κB and caspase-1 in HMC-1 cells.
abstract: Chelidonic acid (CA) is a γ-pyrone which is contained in the rhizome of Chelidonium majus L. It has multiple pharmacological effects including those of a mild analgesic, an antimicrobial, an oncostatic and a central nervous system sedative, but the anti-inflammatory effect of CA and its molecular mechanisms are poorly understood. In this study, we investigated the regulatory mechanism of CA in mast cell-mediated inflammatory response by phorbol 12-myristate 13-acetate and calcium ionophore A23187. The results indicate that CA inhibits the production of interleukin-6 (IL-6) and the expression of IL-6 mRNA through the regulation of nuclear factor-κB. In addition, CA suppresses the activation and expression of caspase-1. These results provide new insights into the pharmacological actions of CA as a potential molecule for use in therapy in mast cell-mediated inflammatory diseases.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyun-Ji
authors.initials: HJ
authors.lastname: Shin
authors.forename: Hye-Lin
authors.initials: HL
authors.lastname: Kim
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Chung
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D020170:Caspase 1 / Q000096:biosynthesis / Q000276:immunology*; D061945:Caspase Inhibitors; D002460:Cell Line; D028281:Chelidonium / Q000737:chemistry; D004789:Enzyme Activation / Q000187:drug effects / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D011714:Pyrans / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D061207:Calcium Ionophores; D002273:Carcinogens; D061945:Caspase Inhibitors; C508600:IL6 protein, human; D015850:Interleukin-6; D016328:NF-kappa B; D011714:Pyrans; D000001:Calcimycin; D020170:Caspase 1; D013755:Tetradecanoylphorbol Acetate; C046884:chelidonic acid