title: Modification of mouse A2M B (620-792) and A2M N (168-230) by malondialdehyde and acetaldehyde attenuates the proteinase and TGF-β1 binding ability of A2MB.
abstract: Acetaldehyde and malondialdehyde react covalently with cellular proteins forming protein-malondialdehyde-acetaldehyde adducts thus modulating their biochemical functions. Alpha-2 macroglobulin, an acute phase protein produced by liver binds to cytokines, growth factors and neutralizes proteinases. In this study we examined the formation of MAA adducts of N-terminal and bait region of mouse A2M and their effect on modulating its proteinase and TGF-β1 binding activities. Adduct formation abrogated the binding of bait region with TGF-β1, trypsin, and elastase. TGF-β1 induced NO production was also suppressed. Acetaldehyde and MDA adduction of A2M may have physiological consequences in alcoholic patients.
authors.affiliation: Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110 007, India. ikbachhal@acbr.du.ac.in
authors.forename: Inderjeet
authors.initials: I
authors.lastname: Kaur
authors.forename: Anju
authors.initials: A
authors.lastname: Katyal
mesh_terms: D000079:Acetaldehyde / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001586:Benzoylarginine Nitroanilide; D001665:Binding Sites; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D008315:Malondialdehyde / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011485:Protein Binding; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000939:Epitopes; D053773:Transforming Growth Factor beta1; D000511:alpha-Macroglobulins; D009569:Nitric Oxide; D008315:Malondialdehyde; D001586:Benzoylarginine Nitroanilide; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase; D014357:Trypsin; D000079:Acetaldehyde