title: Sequence variation and mRNA expression of the TWIST1 gene in cats with mammary hyperplasia and neoplasia.
abstract: In humans, a germline mutation (c.309C>G) in the TWIST1 oncogene may predispose to breast cancer and its expression has been associated with tumour progression and metastasis. In this study, the feline TWIST1 gene was screened for sequence variations in 37 neoplastic and eight hyperplastic mammary gland lesions from cats. In addition, mRNA levels were examined in 15 mammary tumours and three cases of mammary hyperplasia by quantitative real-time reverse-transcriptase PCR. Feline mammary carcinomas had significantly lower levels of expression of TWIST1 mRNA than benign mammary tumours. No variations were identified in the TWIST1 coding region in feline mammary tumours and the mutation described in humans was not detected. However, two germline variants in the TWIST1 gene intron were identified in four and three carcinomas, respectively: GQ167299:g.535delG and GQ167299:g.460C>T. There was no association between these sequence alterations and TWIST1 mRNA levels.
authors.affiliation: Department of Veterinary Clinics, Institute of Biomedical Sciences Abel Salazar, University of Porto (ICBAS - UP), Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal.
authors.forename: Cláudia S
authors.initials: CS
authors.lastname: Baptista
authors.forename: Sara
authors.initials: S
authors.lastname: Santos
authors.forename: António
authors.initials: A
authors.lastname: Laso
authors.forename: Estela
authors.initials: E
authors.lastname: Bastos
authors.forename: Sílvia
authors.initials: S
authors.lastname: Avila
authors.forename: Henrique
authors.initials: H
authors.lastname: Guedes-Pinto
authors.forename: Fátima
authors.initials: F
authors.lastname: Gärtner
authors.forename: Ivo G
authors.initials: IG
authors.lastname: Gut
authors.forename: José L
authors.initials: JL
authors.lastname: Castrillo
authors.forename: Raquel
authors.initials: R
authors.lastname: Chaves
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000235:genetics*; D002415:Cats; D017931:DNA Primers; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051879:Twist-Related Protein 1 / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D051879:Twist-Related Protein 1