title: A new method research for determination of natural pigment crocin yellow in foods by solid-phase extraction ultrahigh pressure liquid chromatography.
abstract: Crocin yellow was determined in soft drinks, sausages and sauces by ultra performance liquid chromatography coupled with ultraviolet detector and analyzed within 5 min using a short analytical column ACQUITY UPLC HSS T3 2.1 x 100 mm 1.8 μm) with gradient elution. An innovative pretreatment method based on homemade macroporous resin solid-phase extraction (SPE) column was established. The SPE column packed with macroporous resins could simplify the sample preparation of multi-matrices and be reused by regeneration steps. The recoveries of crocin yellow added to soft drinks, sausages and sauces at three levels ranged from 81.3% to 106.2%, and relative standard deviations (RSDs) were within 8.8%. The limits of quantitation of soft drinks, sausages and sauces were 0.5 mg/kg, 5 mg/kg and 5 mg/kg, respectively.
authors.affiliation: Analysis and Testing Center, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Wenhan
authors.initials: W
authors.lastname: Yang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.forename: Zhenxia
authors.initials: Z
authors.lastname: Du
mesh_terms: D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D057230:Limit of Detection; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D002338:Carotenoids; C029036:crocin