title: Bostrycin inhibits proliferation of human lung carcinoma A549 cells via downregulation of the PI3K/Akt pathway.
abstract: BACKGROUND
  Bostrycin is a novel compound isolated from marine fungi that inhibits proliferation of many cancer cells. However, the inhibitory effect of bostrycin on lung cancers has not been reported. This study is to investigate the inhibitory effects and mechanism of bostrycin on human lung cancer cells in vitro.
  
  
  METHODS
  We used MTT assay, flow cytometry, microarray, real time PCR, and Western blotting to detect the effect of bostrycin on A549 human pulmonary adenocarcinoma cells.
  
  
  RESULTS
  We showed a significant inhibition of cell proliferation and induction of apoptosis in bostrycin-treated lung adenocarcinoma cells. Bostrycin treatment caused cell cycle arrest in the G0/G1 phase. We also found the upregulation of microRNA-638 and microRNA-923 in bostrycin-treated cells. further, we found the downregulation of p110α and p-Akt/PKB proteins and increased activity of p27 protein after bostrycin treatment in A549 cells.
  
  
  CONCLUSIONS
  Our study indicated that bostrycin had a significant inhibitory effect on proliferation of A549 cells. It is possible that upregulation of microRNA-638 and microRNA-923 and downregulation of the PI3K/AKT pathway proteins played a role in induction of cell cycle arrest and apoptosis in bostrycin-treated cells.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Wei-Sheng
authors.initials: WS
authors.lastname: Chen
authors.forename: Jun-Na
authors.initials: JN
authors.lastname: Hou
authors.forename: Yu-Biao
authors.initials: YB
authors.lastname: Guo
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Yang
authors.forename: Can-Mao
authors.initials: CM
authors.lastname: Xie
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Lin
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: She
mesh_terms: D000230:Adenocarcinoma; D000880:Anthraquinones / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000378:metabolism; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms; D035683:MicroRNAs / Q000096:biosynthesis; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000880:Anthraquinones; D000970:Antineoplastic Agents; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D035683:MicroRNAs; C034287:bostrycin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt