title: Hypotonicity-induced reduction of aquaporin-2 transcription in mpkCCD cells is independent of the tonicity responsive element, vasopressin, and cAMP.
abstract: The syndrome of inappropriate antidiuretic hormone secretion is characterized by excessive water uptake and hyponatremia. The extent of hyponatremia, however, is less than anticipated, which is ascribed to a defense mechanism, the vasopressin-escape, and is suggested to involve a tonicity-determined down-regulation of the water channel aquaporin-2 (AQP2). The underlying mechanism, however, is poorly understood. To study this, we used the mouse cortical collecting duct (mpkCCD) cell line. MpkCCD cells, transfected with an AQP2-promoter luciferase construct showed a reduced and increased AQP2 abundance and transcription following culture in hypotonic and hypertonic medium, respectively. This depended on tonicity rather than osmolality and occurred independently of the vasopressin analog dDAVP, cAMP levels, or protein kinase A activity. Although prostaglandins and nitric oxide reduced AQP2 abundance, inhibition of their synthesis did not influence tonicity-induced AQP2 transcription. Also, cells in which the cAMP or tonicity-responsive element (CRE/TonE) in the AQP2-promoter were mutated showed a similar response to hypotonicity. Instead, the tonicity-responsive elements were pin-pointed to nucleotides -283 to -252 and -157 to -126 bp. In conclusion, our data indicate that hypotonicity reduces AQP2 abundance and transcription, which occurs independently of vasopressin, cAMP, and the known TonE and CRE in the AQP2-promoter. Increased prostaglandin and nitric oxide, as found in vivo, may contribute to reduced AQP2 in vasopressin-escape, but do not mediate the effect of hypotonicity on AQP2 transcription. Our data suggest that two novel segments (-283 to -252 and -157 to -126 bp) in the AQP2-promoter mediate the hypotonicity-induced AQP2 down-regulation during vasopressin-escape.
authors.affiliation: Department of Physiology, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands.
authors.forename: Marleen L A
authors.initials: ML
authors.lastname: Kortenoeven
authors.forename: Michiel
authors.initials: M
authors.lastname: van den Brand
authors.forename: Jack F M
authors.initials: JF
authors.lastname: Wetzels
authors.forename: Peter M T
authors.initials: PM
authors.lastname: Deen
mesh_terms: D000818:Animals; D050034:Antidiuretic Agents / Q000494:pharmacology*; D051399:Aquaporin 2 / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism*; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D009154:Mutation; D009569:Nitric Oxide / Q000096:biosynthesis; D009997:Osmotic Pressure / Q000187:drug effects; D011453:Prostaglandins / Q000096:biosynthesis / Q000235:genetics; D020218:Response Elements / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics
chemical_list: D050034:Antidiuretic Agents; C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D011453:Prostaglandins; D009569:Nitric Oxide; D000242:Cyclic AMP; D003894:Deamino Arginine Vasopressin