title: Lipocalin-type prostaglandin D synthase is associated with coronary vasospasm and vasomotor reactivity in response to acetylcholine.
abstract: BACKGROUND
  Lipocalin-type prostaglandin D synthase (L-PGDS) catalyzes the biosynthesis of PGD(2), which acts as an anticoagulant, vasodilator, and inflammatory mediator. We examined the serum L-PGDS level, coronary macro- and microvasomotor functions, and their relationship in patients with chest pain and angiographically normal coronary arteries.
  
  
  RESULTS
  The study included 96 patients who underwent diagnostic coronary angiography and had angiographically normal coronary arteries. Blood flow of the left anterior descending coronary artery (LAD) was analyzed by Doppler guidewire examination. Serum L-PGDS level was determined by ELISA. Infusion of acetylcholine (ACh) induced vasospasm of the LAD in all patients with vasospastic angina (VSA) (n=45), but in none of the patients without VSA (n=51). There were no significant differences in the baseline clinical characteristics of the nonVSA and VSA groups, except for the frequency of smoking. Serum L-PGDS level in the VSA group was significantly higher than that in the nonVSA group (77.1±4.4 vs. 63.9±2.5 µg/dl, P<0.01). Significant negative correlations were observed between the degree of LAD vasomotion in response to ACh and serum L-PGDS level (3 µg/min: r=-0.33; 10 µg/min: r=-0.35; 30 µg/min: r=-0.33, P<0.01).
  
  
  CONCLUSIONS
  The L-PGDS level was elevated in patients with VSA and was associated with epicardial coronary vasomotion in response to ACh.
authors.affiliation: Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Science, Seta Tsukinowa, Otsu 520-2192, Japan. tetsuyam@belle.shiga-med.ac.jp
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Eguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oda
authors.forename: Tetsunobu
authors.initials: T
authors.lastname: Yamane
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tarutani
authors.forename: Tomoya
authors.initials: T
authors.lastname: Ozawa
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakae
authors.forename: Minoru
authors.initials: M
authors.lastname: Horie
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
mesh_terms: D000109:Acetylcholine / Q000008:administration & dosage*; D000787:Angina Pectoris / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000628:therapy; D017023:Coronary Angiography / Q000379:methods; D003329:Coronary Vasospasm / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000628:therapy; D003331:Coronary Vessels / Q000201:enzymology; D005260:Female; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000097:blood*; D054834:Lipocalins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015230:Prostaglandin D2 / Q000096:biosynthesis; D014661:Vasoconstriction / Q000187:drug effects*; D014665:Vasodilator Agents / Q000008:administration & dosage*
chemical_list: D054834:Lipocalins; D014665:Vasodilator Agents; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase; D000109:Acetylcholine; D015230:Prostaglandin D2