title: Antioxidative ability of chicken myofibrillar protein developed by glycosylation and changes in the solubility and thermal stability.
abstract: Myofibrillar protein prepared from chicken breast muscle was incubated with several concentrations of glucose or maltose for 6 h at 60 °C and 35% relative humidity in order to obtain glycosylated chicken protein. When the ratio of the weights of the myofibrillar protein and glucose or maltose had respectively reached 1:6 or 1:3-5, the solubility of each type of glycosylated chicken protein in a 0.1 M NaCl solution was exceeded by about 60%, although the myofibrillar protein was insoluble in a low ionic strength solution. Moreover, when the myofibril and maltose reaction (myofibril:maltose = 1:4) was extended to 36 h, the glycosylated protein did not undergo denaturation when held at 50 °C for 2 h, while it also exhibited an antioxidative function against superoxide anion radicals.
authors.affiliation: Department of Food Science and Nutrition, Faculty of Human Life and Science, Doshisha Women's College of Liberal Arts, Kyoto, Japan. knishimu@dwc.doshisha.ac.jp
authors.forename: Kimio
authors.initials: K
authors.lastname: Nishimura
authors.forename: Miki
authors.initials: M
authors.lastname: Murakoshi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Katayama
authors.forename: Hiroki
authors.initials: H
authors.lastname: Saeki
mesh_terms: D000818:Animals; D002645:Chickens; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism*; D019270:Fructosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D009124:Muscle Proteins / Q000737:chemistry* / Q000378:metabolism*; D009210:Myofibrils; D009994:Osmolar Concentration; D055550:Protein Stability; D012995:Solubility; D013481:Superoxides / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D016166:Free Radical Scavengers; D009124:Muscle Proteins; D013481:Superoxides; D019270:Fructosamine; D008320:Maltose; D005947:Glucose; D008239:Lysine