title: Rapid method for the determination of capsaicin and dihydrocapsaicin in Gochujang using ultra-high-performance liquid chromatography.
abstract: A sensitive and specific heating block method coupled with ultra-HPLC (u-HPLC) was developed for the analysis of capsaicin in Gochujang and validated by comparing with a conventional HPLC (AOAC Method 995.03). The method validation parameters yielded good results, including linearity, precision, accuracy, and recovery. The u-HPLC separation was performed on a reversed C18 column (50 x 2 mm id, particle size 2 microm), followed by fluorescence detection (excitation 280 nm, emission 325 nm). Methanol was used as the extracting solvent, and the amount of sample taken was approximately 0.2 g; the optimum amount of extraction solvent and extraction time were 15 mL and 1 h, respectively. The recovery of capsaicin in Gochujang was more than 93%, and the LOD and LOQ of the u-HPLC analysis were 0.05 and 0.16 microg/g for capsaicin and 0.05 and 0.16 microg/g for dihydrocapsaicin. The calibration graphs for capsaicin and dihydrocapsaicin were linear from 0.2 to 10.0 microg/mL for u-HPLC. The interday and intraday precisions (RSD values) were < 6.27%.
authors.affiliation: Korea Food Research Institute, Food Analysis Center, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea. jhkfri@kfri.re.kr
authors.forename: Jaeho
authors.initials: J
authors.lastname: Ha
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Seo
authors.forename: You-Shin
authors.initials: YS
authors.lastname: Shim
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Nam
authors.forename: Homoon
authors.initials: H
authors.lastname: Seog
authors.forename: Masahito
authors.initials: M
authors.lastname: Ito
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D002138:Calibration; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents; D056910:Republic of Korea; D012997:Solvents
chemical_list: D007202:Indicators and Reagents; D012997:Solvents; D002211:Capsaicin; C012906:dihydrocapsaicin