title: Unique gene expression patterns indicate microglial contribution to neural stem cell recovery following irradiation.
abstract: Ionizing radiation results in damage to neural stem cells and reduced neurogenesis. The aim of the present study was to determine intrinsic and extrinsic factors that influence neural stem cell survival following irradiation, using qPCR. Gene expression of hippocampal and SVZ neurospheres were analyzed following irradiation, and results demonstrated that irradiated hippocampal and SVZ stem cells displayed similar gene expression profiles for intrinsic genes. Irradiated microglia (extrinsic factor) isolated from the SVZ exhibited increased gene expression of growth factors involved in stem cell maintenance, proliferation, and survival. However, microglial genes in the irradiated hippocampus responded less favorably with respect to stem cell recovery. This might explain the superior recovery of SVZ compared to hippocampal stem cells following in vivo irradiation. In addition, our results show that a combination of growth factors, which were upregulated in SVZ microglia, increased the proliferation and decreased cell death of irradiated neurospheres in vitro.
authors.affiliation: Center for Brain Repair and Rehabilitation, Institute for Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Nina A K
authors.initials: NA
authors.lastname: Hellström
authors.forename: Olle R
authors.initials: OR
authors.lastname: Lindberg
authors.forename: Anders
authors.initials: A
authors.lastname: Ståhlberg
authors.forename: John
authors.initials: J
authors.lastname: Swanpalmer
authors.forename: Milos
authors.initials: M
authors.lastname: Pekny
authors.forename: Klas
authors.initials: K
authors.lastname: Blomgren
authors.forename: H Georg
authors.initials: HG
authors.lastname: Kuhn
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D016133:Polymerase Chain Reaction / Q000379:methods; D025341:Principal Component Analysis; D011839:Radiation, Ionizing; D051381:Rats; D017208:Rats, Wistar
chemical_list: D036341:Intercellular Signaling Peptides and Proteins