title: Endophytic fungi from Dragon's blood specimens: isolation, identification, phylogenetic diversity and bioactivity.
abstract: Endophytic fungi from Dragon's blood specimens of different locations of China were characterized taxonomically and investigated concerning their antimicrobial and antitumor activity against six pathogenic microbes and five tumor cells. A total of 49 endophytic fungi were obtained from Dragon's blood materials of Dracaena spp., 18 taxa were represented by 43 (87.8%) isolates and only six (12.2%) isolates were unknown. Twenty (40.8%) of the isolates displayed antimicrobial activity against at least one pathogenic microorganism. Three isolates YNDC07, BJDC06 and BJDC09 displayed significant antimicrobial activities against Staphylococcus aureus, Cryptococcus neoformans and Aspergillus fumigates, respectively. The results of antitumor activity by the MTT assay revealed that 26.5%, 69.4%, 48.9%, 6.1% and 42.9% of isolate fermentation broths displayed growth inhibition on HepG2 cells, SKVO3 cells, MCF7 cells, HL-60 cells and 293-T cells, respectively. HNDC09 and HNDC10 showed very strong antitumor activity against MCF7 and 293-T, respectively. The results showed that endophytic fungi in Dragon's blood samples were valuable in screening antitumor and antimicrobial bioactivity agents.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Jin-long
authors.initials: JL
authors.lastname: Cui
authors.forename: Shun-xing
authors.initials: SX
authors.lastname: Guo
authors.forename: Hailing
authors.initials: H
authors.lastname: Dong
authors.forename: Peigen
authors.initials: P
authors.lastname: Xiao
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects; D002681:China; D003455:Cryptococcus neoformans / Q000187:drug effects; D004271:DNA, Fungal / Q000235:genetics; D031409:Dracaena / Q000737:chemistry* / Q000382:microbiology*; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018922:HL-60 Cells; D056945:Hep G2 Cells; D006801:Humans; D008516:Medicine, Chinese Traditional; D010802:Phylogeny; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D004271:DNA, Fungal; D010936:Plant Extracts