title: Identification and assay of allosteric regulators of S-adenosylmethionine decarboxylase.
abstract: Polyamine biosynthesis is extensively regulated in cells by multiple mechanisms, including regulation of enzyme activity posttranslationally. The identified regulatory factors include both small molecules and regulatory proteins, and the mechanisms vary in different species across the evolutionary tree. Based on this diversity of mechanism, it is likely that regulatory factors of the pathway remain unidentified in many species. This article focuses on methods for identifying novel regulatory factors of polyamine biosynthesis as illustrated by the discovery of a novel protein activator of the key biosynthetic enzyme S-adenosylmethionine decarboxylase in the protozoan trypanosomatid parasites.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA.
authors.forename: Erin K
authors.initials: EK
authors.lastname: Willert
authors.forename: Lisa N
authors.initials: LN
authors.lastname: Kinch
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Phillips
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000494:Allosteric Regulation / Q000187:drug effects; D055162:Biocatalysis / Q000187:drug effects; D053898:Biosynthetic Pathways / Q000187:drug effects; D015153:Blotting, Western; D020134:Catalytic Domain; D002457:Cell Extracts; D002850:Chromatography, Gel; D020536:Enzyme Activators / Q000494:pharmacology; D057075:Enzyme Assays / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry / Q000302:isolation & purification; D011485:Protein Binding / Q000187:drug effects; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000378:metabolism; D014345:Trypanosoma / Q000201:enzymology*; D014461:Ultracentrifugation
chemical_list: D002457:Cell Extracts; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D011073:Polyamines; D011994:Recombinant Proteins; D012437:Adenosylmethionine Decarboxylase