title: Acid-sensing ion channels contribute to the effect of acidosis on the function of dendritic cells.
abstract: As an H(+)-gated subgroup of the degenerin/epithelial Na(+) channel family, acid-sensing ion channels (ASICs) were reported to be involved in various physiological and pathological processes in neurons. However, little is known about the role of ASICs in the function of dendritic cells (DCs). In this study, we investigated the expression of ASICs in mouse bone marrow-derived DCs and their possible role in the function of DCs. We found that ASIC1, ASIC2, and ASIC3 are expressed in DCs at the mRNA and protein levels, and extracellular acid can evoke ASIC-like currents in DCs. We also demonstrated that acidosis upregulated the expression of CD11c, MHC class II, CD80, and CD86 and enhanced the Ag-presenting ability of DCs via ASICs. Moreover, the effect of acidosis on DCs can be abolished by the nonsteroidal anti-inflammatory drugs ibuprofen and diclofenac. These results suggest that ASICs are involved in the acidosis-mediated effect on DC function.
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tong
authors.forename: Wen-Ning
authors.initials: WN
authors.lastname: Wu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Kong
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Du
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Fang
authors.initials: F
authors.lastname: Zheng
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tan
authors.forename: Feili
authors.initials: F
authors.lastname: Gong
mesh_terms: D062565:Acid Sensing Ion Channels; D000138:Acidosis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D005110:Extracellular Space / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000096:biosynthesis / Q000502:physiology*
chemical_list: C568267:ASIC1 protein, mouse; C568265:ASIC2 protein, mouse; C568269:ASIC3 protein, rat; D062565:Acid Sensing Ion Channels; C568268:Asic1 protein, rat; D009419:Nerve Tissue Proteins; D015222:Sodium Channels