title: Fluorescence determination of DNA using the gatifloxacin-europium(III) complex.
abstract: A method for the determination of DNA based on the fluorescence intensity of the gatifloxacin-europium(III) (GFLX-Eu(3+)) complex that could be enhanced by DNA was developed. The GFLX-Eu(3+) complex showed an up to 6-fold enhancement of luminescence intensity after adding DNA. Under the optimized experimental conditions, the system exhibited a linear relationship between the enhanced fluorescence intensity and the concentration of calf thymus DNA (ctDNA) over the range from 1.0 × 10(-8) to 1.5 × 10(-6) g mL(-1), with a correlation coefficient (R) of 0.997, and the detection limit (3σ) of the method was determined as 6.0 × 10(-9) g mL(-1). The mechanism of the fluorescence enhancement effect was also discussed.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University , 250012 Jinan, People's Republic of China. wangl-sdu@sdu.edu.cn
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Changchuan
authors.initials: C
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D005063:Europium; D024841:Fluoroquinolones; D000077734:Gatifloxacin; D006863:Hydrogen-Ion Concentration; D049408:Luminescent Agents; D009994:Osmolar Concentration; D012996:Solutions; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D024841:Fluoroquinolones; D049408:Luminescent Agents; D012996:Solutions; D005063:Europium; D004247:DNA; D000077734:Gatifloxacin