title: TGF-β1 gene polymorphism in renal transplant patients with and without gingival overgrowth.
abstract: BACKGROUND
  The incidence of gingival overgrowth among renal transplant patients treated with cyclosporine A ranges from 13% to 84.6%, and the overgrowth is not only esthetic but also a medical problem. We studied the determination of association between TGF-β1 (TGFB1) gene polymorphism and gingival overgrowth in kidney transplant patients medicated with cyclosporin A.
  
  
  METHODS
  Eighty-four kidney transplant patients with gingival overgrowth and 140 control transplant patients without overgrowth were enrolled into the case control study. TGFB1 polymorphism was determined using the PCR-RFLP assay for +869T > C in codon 10 and +915G > C in codon 25 as well as TaqMan real-time PCR assays for promoter -800G>A and -509C > T SNPs.
  
  
  RESULTS
  In kidney transplant patients suffering from gingival overgrowth, mean score of gingival overgrowth was 1.38 ± 0.60, whereas in control subjects it was 0.0. The patients with gingival overgrowth were characterized by similar distribution of TGFB1 genotypes and allele in comparison to subjects without gingival overgrowth. Among 16 potentially possible haplotypes of TGFB1 gene, only four were observed in the studied sample of kidney transplant patients: G_C_T_G, G_T_C_G, G_C_C_C, and A_C_T_G, with similar frequency in patients with and without gingival overgrowth.
  
  
  CONCLUSIONS
  No association between the TGFB1 gene polymorphism and gingival overgrowth was revealed in kidney transplant patients administered cyclosporine A.
authors.affiliation: Department of Pharmacology, Pomeranian Medical University, Szczecin, Poland. drozdzik@sci.pam.szczecin.pl
authors.forename: M
authors.initials: M
authors.lastname: Kozak
authors.forename: M
authors.initials: M
authors.lastname: Kurzawski
authors.forename: A
authors.initials: A
authors.lastname: Wajda
authors.forename: J
authors.initials: J
authors.lastname: Lapczuk
authors.forename: M
authors.initials: M
authors.lastname: Lipski
authors.forename: K
authors.initials: K
authors.lastname: Dziewanowski
authors.forename: M
authors.initials: M
authors.lastname: Drozdzik
mesh_terms: D000225:Adenine; D000293:Adolescent; D000328:Adult; D000368:Aged; D001120:Arginine / Q000235:genetics; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics; D016572:Cyclosporine / Q000008:administration & dosage / Q000627:therapeutic use; D003596:Cytosine; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D019214:Gingival Overgrowth / Q000209:etiology*; D006147:Guanine; D006239:Haplotypes; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000627:therapeutic use; D016030:Kidney Transplantation; D007930:Leucine / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011392:Proline / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D013941:Thymine; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D055815:Young Adult
chemical_list: D003062:Codon; D007166:Immunosuppressive Agents; D053773:Transforming Growth Factor beta1; D006147:Guanine; D016572:Cyclosporine; D003596:Cytosine; D001120:Arginine; D011392:Proline; D007930:Leucine; D000225:Adenine; D013941:Thymine