title: Applicability of RNA standards for evaluating RT-qPCR assays and platforms.
abstract: The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.
authors.affiliation: LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK.
authors.forename: Alison S
authors.initials: AS
authors.lastname: Devonshire
authors.forename: Ramnath
authors.initials: R
authors.lastname: Elaswarapu
authors.forename: Carole A
authors.initials: CA
authors.lastname: Foy
mesh_terms: D057230:Limit of Detection; D012313:RNA / Q000032:analysis*; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*
chemical_list: D012313:RNA