title: Enhanced gene delivery using biodegradable poly(ester amine)s (PEAs) based on low-molecular-weight polyethylenimine and poly(epsilon-caprolactone)-pluronic-poly(epsilon-caprolactone).
abstract: In this paper, the poly(ester amine)s (PEAs) were successfully prepared from low-molecular-weight PEI (Mn = 2000) and Poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCFC) copolymers using isophorone diisocyanate (IPDI) as cross-linker. The obtained PEAs copolymers are biodegradable and water-soluble. The PEAs/DNA complexes showed effective and stable DNA condensation with the particle size < or = 200 nm and zeta potential > or =10 mV, indicating its potential for intracellular delivery. Compared to the unmodified low-molecular-weight PEI, PEAs displayed similarly low cytotoxicity in all two cell lines (293T: Human kidney carcinoma, HUVEC: Human umbilical vein Endothelial cell) and revealed much higher transfection efficiency in 293T cell lines. Therefore these PEAs might be a novel safe and efficient polymeric gene delivery vectors.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liu
authors.forename: Xiujun
authors.initials: X
authors.lastname: Yu
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Guo
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Wang
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D020341:Absorbable Implants; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry*; D018014:Gene Transfer Techniques; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D010316:Particle Size; D011060:Poloxalene / Q000031:analogs & derivatives / Q000737:chemistry; D020442:Poloxamer / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D011094:Polyethyleneimine / Q000737:chemistry* / Q000494:pharmacology; D014471:Umbilical Veins / Q000187:drug effects
chemical_list: D004337:Drug Carriers; D011073:Polyamines; D011091:Polyesters; C516446:poly(caprolactone-block-ethyleneimine); C547086:poly(epsilon-caprolactone)-pluronic-poly(epsilon-caprolactone)-dimethacrylate; D020442:Poloxamer; C016240:polycaprolactone; D011094:Polyethyleneimine; D011060:Poloxalene