title: High-throughput assay of oleopentanedialdheydes in extra virgin olive oil by the UHPLC-ESI-MS/MS and isotope dilution methods.
abstract: The quality of extra virgin olive oil is associated with the presence of microcomponents whose healing effects have been proved in some special cases. The enzymatic hydrolysis of oleuropein and ligstroside, and of their demethylated analogues, affords four different pentanedialdehydes, and for one of which, 2-(4-hydroxyphenyl)ethyl (3S,4E)-4-formyl-3-(2-oxoethyl)hex-4-enoate, also known as oleocanthal, an anti-inflammatory effect was quite recently carefully assessed. Extra virgin olive oil is now worldwide considered as a functional food whose daily intake, as for the Mediterranean diet, helps consumers in keeping a constant level of nonsteroidal anti-inflammatory drug (NSAID) in the blood. The presence of these active principles provides, therefore, olive oil with an important added value. In the framework of the actions of the recently funded Agrifood Regional Center, which should coordinate the scientific research and production worlds, an absolute analytical method was developed for the mass spectrometric detection of the two most abundant NSAIDs, Tyr-OLPD and HTyr-OLPD (oleopentanedialdehydes (OLPDs) conjugated to p-hydroxyphenylethanol and 3,4-dihydroxyphenylethanol, respectively), by UHPLC-ESI-MS/MS.
authors.affiliation: Dipartimento di Chimica, Università della Calabria, via P. Bucci, cubo 12/C, I-87030 Arcavacata di Rende (CS), Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Anna
authors.initials: A
authors.lastname: Napoli
authors.forename: Monica
authors.initials: M
authors.lastname: Nardi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D057230:Limit of Detection; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D000447:Aldehydes; D000069463:Olive Oil; D010938:Plant Oils