title: Quality control of pharmaceutical formulations containing cisplatin, carboplatin, and oxaliplatin by micellar and microemulsion electrokinetic chromatography (MEKC, MEEKC).
abstract: A micellar electrokinetic chromatography (MEKC) method was developed for the determination of cisplatin, carboplatin, and oxaliplatin in pharmaceutical formulations. The background electrolyte consisted of a phosphate buffer (pH 7.0; 25 mM) with sodium dodecyl sulfate (80 mM). The applied voltage was 30 kV and the sample injection was performed in the hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50 μm and a total length of 64.5 cm. The detection of target compounds was performed at 200 nm. Under these conditions, a complete separation of cisplatin, carboplatin and oxaliplatin was achieved in less than 10 min. The MEKC-UV method was validated and trueness values between 99.7% and 100.8% were obtained with repeatability and intermediate precision values of 0.7-1.4% and 1.1-1.7%, respectively for the three drugs. This method was found appropriate for controlling pharmaceutical formulations containing platinum complexes and successfully applied in quality control at the Geneva University Hospitals.
authors.affiliation: Geneva University Hospital (HUG), Pharmacy, and School of Pharmaceutical Sciences-EPGL, University of Geneva, 20 Bd d'Yvoy, 1211 Geneva 4, Switzerland.
authors.forename: Susanne
authors.initials: S
authors.lastname: Nussbaumer
authors.forename: Sandrine
authors.initials: S
authors.lastname: Fleury-Souverain
authors.forename: Julie
authors.initials: J
authors.lastname: Schappler
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Veuthey
authors.forename: Pascal
authors.initials: P
authors.lastname: Bonnabry
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis*; D016190:Carboplatin / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D002945:Cisplatin / Q000032:analysis*; D004655:Emulsions; D008823:Micelles; D009944:Organoplatinum Compounds / Q000032:analysis*; D000077150:Oxaliplatin; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D004655:Emulsions; D008823:Micelles; D009944:Organoplatinum Compounds; D004364:Pharmaceutical Preparations; D000077150:Oxaliplatin; D016190:Carboplatin; D002945:Cisplatin