title: Characterisation of clinical and food animal Escherichia coli isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A.
abstract: Seven phylogroup A CTX-M-15-producing Escherichia coli isolates recovered from clinical and meat samples were further characterised. All of them belonged to sequence type ST410. Only 2 of the 22 virulence genes investigated were detected. All isolates carried the fimH gene encoding type 1 fimbriae, and five isolates harboured the iucD gene encoding aerobactin siderophore. A group of five isolates showed 81.2% similarity by pulsed-field gel electrophoresis (PFGE), comprising three clinical isolates belonging to ONT:H9 and two food isolates belonging to O55:H9. Different HpaI digestion patterns were observed for plasmids, but all of them belonged to IncFIB group and harboured bla(CTX-M-15) associated with bla(OXA-1), bla(TEM), tetA, catB3 and aac(6')-Ib surrounded by an identical genetic environment. These findings showed the possibility of lateral gene transfer of bla(CTX-M-15) as well as other antibiotic resistance determinants between low-virulence food and clinical isolates.
authors.affiliation: University Hospital Virgen Macarena, Seville, Spain. llopez@us.es
authors.forename: Lorena
authors.initials: L
authors.lastname: López-Cerero
authors.forename: Pilar
authors.initials: P
authors.lastname: Egea
authors.forename: Lara
authors.initials: L
authors.lastname: Serrano
authors.forename: Dolores
authors.initials: D
authors.lastname: Navarro
authors.forename: Azucena
authors.initials: A
authors.lastname: Mora
authors.forename: Jorge
authors.initials: J
authors.lastname: Blanco
authors.forename: Yohei
authors.initials: Y
authors.lastname: Doi
authors.forename: David L
authors.initials: DL
authors.lastname: Paterson
authors.forename: Jesús
authors.initials: J
authors.lastname: Rodríguez-Baño
authors.forename: Alvaro
authors.initials: A
authors.lastname: Pascual
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D005516:Food Microbiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D017931:DNA Primers; D001618:beta-Lactamases