title: Genomic approaches for the discovery of CFTR regulatory elements.
abstract: Non-coding regions of the human genome contain vast regulatory potential that contributes to the coordination of gene expression. Indeed, regulatory elements can reside large genomic distances from the promoters of genes they control. Here we describe approaches recently used to identify functional elements within the complex CFTR locus.
authors.affiliation: Human Molecular Genetics Program, Children's Memorial Research Center, Chicago, IL, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Ott
authors.forename: Ann
authors.initials: A
authors.lastname: Harris
mesh_terms: D001665:Binding Sites / Q000235:genetics; D015139:Blotting, Southern; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006801:Humans; D038101:Insulator Elements / Q000235:genetics; D007438:Introns / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002843:Chromatin; D014157:Transcription Factors; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004247:DNA; D003850:Deoxyribonuclease I