title: Genetic diversity of Imjin virus in the Ussuri white-toothed shrew (Crocidura lasiura) in the Republic of Korea, 2004-2010.
abstract: Recently, Imjin virus (MJNV), a genetically distinct hantavirus, was isolated from lung tissues of the Ussuri white-toothed shrew (Crocidura lasiura) captured near the demilitarized zone in the Republic of Korea. To clarify the genetic diversity of MJNV, partial M- and L-segment sequences were amplified from lung tissues of 12 of 37 (32.4%) anti-MJNV IgG antibody-positive Ussuri white-toothed shrews captured between 2004 and 2010. A 531-nucleotide region of the M segment (coordinates 2,255 to 2,785) revealed that the 12 MJNV strains differed by 0-12.2% and 0-2.3% at the nucleotide and amino acid levels, respectively. A similar degree of nucleotide (0.2-11.9%) and amino acid (0-3.8%) difference was found in a 632-nucleotide length of the L segment (coordinates 962 to 1,593) of nine MJNV strains. Phylogenetic analyses, based on the partial M and L segments of MJNV strains generated by the neighbor-joining and maximum likelihood methods, showed geographic-specific clustering, akin to the phylogeography of rodent-borne hantaviruses.
authors.affiliation: Department of Microbiology, College of Medicine, and Institute for Viral Diseases, Korea University, 5-Ga, Anam-dong, Sungbuk-gu, Seoul 136-705, Korea.
authors.forename: Se Hun
authors.initials: SH
authors.lastname: Gu
authors.forename: Hae Ji
authors.initials: HJ
authors.lastname: Kang
authors.forename: Luck Ju
authors.initials: LJ
authors.lastname: Baek
authors.forename: Ji Yun
authors.initials: JY
authors.lastname: Noh
authors.forename: Heung-Chul
authors.initials: HC
authors.lastname: Kim
authors.forename: Terry A
authors.initials: TA
authors.lastname: Klein
authors.forename: Richard
authors.initials: R
authors.lastname: Yanagihara
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Song
mesh_terms: D000818:Animals; D007312:Eulipotyphla / Q000821:virology*; D014644:Genetic Variation; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins