title: [Comparative study on metabonomics and on liver and kidney toxicity of Aristolochia fangchi and Stephania tetrandra].
abstract: OBJECTIVE
  Combined the blood biochemical markers, the renal histopathological changes and the metabonomics profile were investigated to study the toxicity differences between Aristolochia fangchi and Stephania tetrandra.
  
  
  METHODS
  Ten rats were randomly selected from 70 male Wistar rats as blank control group. The remaining 60 rats were divided into three groups. The two treated groups were orally administrated by 8.1 g x kg(-1) of A. fangchi and S. tetrandra respectively and the control group by equal volume of distilled water for 4weeks. Before the administrated and every 2 weeks, urine and plasma were collected and their 1H-NMR spectra were acquired, and then subjected to data process and PCA. Blood biochemical analysis and histopathological examination were carried out.
  
  
  RESULTS
  On the 2nd weekend, the BUN of the two treated groups, the AST of A. fangchi group were all markedly higher than that of the control group (P < 0.05). Compared with the A. fangchi group, the SCr higher in the S. tetrandra group (P < 0.05). The kidney pathological changes were apparently in the two treated groups and the pathological changes in the liver apparently in the S. tetrandra group. Along with the lasting of administration to the 4th week, the BUN, ALT and AST of the two treated groups, the SCr of A. fangchi group were all significantly higher than that of the control group (P < 0.01). The renal and liver injuries in the two treated groups were all become more seriously. Comparing the A. fangchi group, the BUN, SCr and AST were all higher in the S. tetrandra group (P < 0.05). Compared with control group, the urinary concentrations of citrate, 2-oxo-glutarate, taurine, hippurate, TMAO, creatine and the plasma concentrations of 3-D-hydroxybutyrate, acetone, NAC, OAC, creatinine were all changed.
  
  
  CONCLUSIONS
  The A. fangchi and S. tetrandra all can induce the renal and liver lesion and its seriousness is correspondent to the lasting of administration. The liver and kidney toxicity of S. tetrandra are all more serious than the A. fangchi.
authors.affiliation: Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China. liangqi5277@yahoo.com.cn
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
authors.forename: Cheng
authors.initials: C
authors.lastname: Ni
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Yan
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Meijuan
authors.initials: M
authors.lastname: Yang
authors.forename: Shuangqing
authors.initials: S
authors.lastname: Peng
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D030022:Aristolochia / Q000737:chemistry*; D001774:Blood Chemical Analysis; D064420:Drug-Related Side Effects and Adverse Reactions; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000009:adverse effects* / Q000378:metabolism; D007668:Kidney / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D055432:Metabolomics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D031609:Stephania tetrandra / Q000737:chemistry*; D014556:Urine / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal