title: Impact of lipid substitution on assembly and delivery of siRNA by cationic polymers.
abstract: Characterization of a polymer library engineered to enhance their ability to protect and deliver their nucleotide cargo to the cells is reported. The ζ-potential continuously increased with higher polymer:siRNA weight ratio, and the ζ-potential of lipid-modified polymers:siRNA complexes were higher than PEI2 at all ratios. At polymer:siRNA ratio of 1:1, all lipid-substituted polymers showed complete protection against degradation. Lipid-modified polymers significantly increased the cellular uptake of siRNA complexes and down-regulation of GAPDH and P-gp (max. 66% and 67%, respectively). The results indicate that hydrophobic modification of low molecular PEI could render this otherwise ineffective polymer to a safe effective delivery system for intracellular siRNA delivery and protein silencing.
authors.affiliation: Department of Chemical & Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB, Canada.
authors.forename: Hamidreza Montazeri
authors.initials: HM
authors.lastname: Aliabadi
authors.forename: Breanne
authors.initials: B
authors.lastname: Landry
authors.forename: Remant K
authors.initials: RK
authors.lastname: Bahadur
authors.forename: Artphop
authors.initials: A
authors.lastname: Neamnark
authors.forename: Orawan
authors.initials: O
authors.lastname: Suwantong
authors.forename: Hasan
authors.initials: H
authors.lastname: Uludağ
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004337:Drug Carriers; D004355:Drug Stability; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry; D054852:Small Molecule Libraries; D014162:Transfection / Q000379:methods*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004337:Drug Carriers; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D008055:Lipids; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D034741:RNA, Small Interfering; D054852:Small Molecule Libraries