title: Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles.
abstract: Target gene transfection for desired cell differentiation has recently become a major issue in stem cell therapy. For the safe and stable delivery of genes into human mesenchymal stem cells (hMSCs), we employed a non-viral gene carrier system such as polycataionic polymer, poly(ethyleneimine) (PEI), polyplexed with a combination of SOX5, 6, and 9 fused to green fluorescence protein (GFP), yellow fluorescence protein (YFP), or red fluorescence protein (RFP) coated onto PLGA nanoparticles. The transfection efficiency of PEI-modified PLGA nanoparticle gene carriers was then evaluated to examine the potential for chondrogenic differentiation by carrying the exogenous SOX trio (SOX5, 6, and 9) in hMSCs. Additionally, use of PEI-modified PLGA nanoparticle gene carriers was evaluated to investigate the potential for transfection efficiency to increase the potential ability of chondrogenesis when the trio genes (SOX5, 6, and 9) polyplexed with PEI were delivered into hMSCs. SOX trio complexed with PEI-modified PLGA nanoparticles led to a dramatic increase in the chondrogenesis of hMSCs in in vitro culture systems. For the PEI/GFP and PEI/SOX5, 6, and 9 genes complexed with PLGA nanoparticles, the expressions of GFP as reporter genes and SOX9 genes with PLGA nanoparticles showed 80% and 83% of gene transfection ratios into hMSCs two days after transfection, respectively.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 606-16 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Han Na
authors.initials: HN
authors.lastname: Yang
authors.forename: Dae Gyun
authors.initials: DG
authors.lastname: Woo
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Jeon
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Do
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Lim
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
mesh_terms: D020219:Chondrogenesis / Q000187:drug effects; D006801:Humans; D007097:Imines / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011095:Polyethylenes / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D055758:SOX9 Transcription Factor / Q000737:chemistry / Q000494:pharmacology*; D055755:SOXD Transcription Factors / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D007097:Imines; D011095:Polyethylenes; C490301:SOX5 protein, human; D055758:SOX9 Transcription Factor; D055755:SOXD Transcription Factors; C505405:poly(ethylene imine); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid