title: [Study on the effects of p53 deletion and mutation on malignant phenotype of human lung cancer cell line].
abstract: BACKGROUND
  To study the inhibition effects of both extraneous right sense and antisense p53 on malignant phenotype of human lung cancer cell line.
  
  
  METHODS
  The named 801D cell line with p53 deletion and mutation at 248 code was selected as a model in vitro. The recombined plasmid pEGFP-p53(RS) and pEGFP-p53(AS) were constructed. The extraneous gene was detected by PCR. The p53 mutation protein was examined by immunohistochemical stain of p53 antibody. The inhibition effect of extraneous p53 on tumor growth in vitro were determined by clonogenic survival assay. FCM analysis was carried out in cells. The inhibition effect on malignant growth of extraneous p53 in vivo was observed by heteroplastic transplant on nude mouse.
  
  
  RESULTS
  The transfected cell lines, pEGFP-p53(AS)-801D, pEGFP-p53(RS)-801D and pEGFP-801D were established. Presence of extraneous p53 and neo genes in pEGFP-p53(AS)-801D and pEGFP-p53(RS)-801D was proved by PCR and green fluorescence was found out in those cells under the microscope. Mutant protein in pEGFP-p53(AS)-801D was negative by immunohistochemical stain. The malignant growth of these transfected cell lines was inhibited comparing with parents in vivo and in vitro. Inhibition rate of colony formation was 62.0% for pEGFP-p53(AS)-801D and 80.8% for pEGFP-p53(RS)-801D. The tumorigenicity in nude mice was suppressed. Inhibition effects of extraneous right sense p53 on malignant growth of 801D was more distinct. FCM analysis showed that pEGFP-p53(AS)-801D cells were arrested at G1 phase.
  
  
  CONCLUSIONS
  The transfected cell lines with extraneous right sense and antisense p53 appear that malignant growth can be inhibited in vivo and in vitro.
authors.affiliation: The Laboratory of Cellular and Molecular Biology, Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing 101149, P.R.China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Baitang
authors.initials: B
authors.lastname: Lai
authors.forename: Jinzhao
authors.initials: J
authors.lastname: Li
authors.forename: Xuehui
authors.initials: X
authors.lastname: Yang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.forename: Wentao
authors.initials: W
authors.lastname: Yue
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhan
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang