title: Histochemical and immunohistochemical study in melasma: evidence of damage in the basal membrane.
abstract: The pathogenesis of melasma has not been clearly elucidated. Using Fontana Masson; diastase-resistant periodic acid-Schiff stains; and immunohistochemistry to stem cell factor (SCF), its receptor c-kit, anti-mast cell tryptase, and anti-collagen type IV antibody, we evaluated melasma lesions and compared them with perilesional skin and photoprotected skin. Samples were taken from lesional and photoprotected nonlesional skin in 24 patients. In other 24 patients, we took biopsies of lesional and perilesional skin. With Fontana Masson, we observed many pigmented basal cells protruding into the dermis of the melasma skin. Periodic acid-Schiff stain and anti-collagen type IV showed damage on the basal membrane in 95.5% and 83%, respectively, in melasma lesion. The immunoreactivity of SCF and the prevalence of mast cells were increased in the dermis of melasma compared with perilesional dermis. The expression of c-kit was significantly increased at lesional epidermis; a frequent protrusion of c-kit-positive basal cells into the dermis was evident in 70% versus that in 29% of perilesional skin. The expression of c-kit was increased at lesional dermis of melasma compared with perilesional skin. We found a low correlation between c-kit expression and prevalence of mast cells; these were increased in melasma skin. The results may suggest a role of SCF, c-kit, and mast cells in the pathogenesis of melasma. We were surprised by the unexpected evidence of damage to basal membrane (BM), which could facilitate the fall or the migration of active melanocytes and melanin into the dermis allowing the constant hyperpigmentation in melasma.
authors.affiliation: Department of Dermatology, Hospital Central Dr. Ignacio Morones Prieto, Universidad Autónoma de San Luis Potosí, S.L.P., (UASLP), México. torresmab@yahoo.com.mx
authors.forename: Bertha
authors.initials: B
authors.lastname: Torres-Álvarez
authors.forename: Iraida G
authors.initials: IG
authors.lastname: Mesa-Garza
authors.forename: Juan P
authors.initials: JP
authors.lastname: Castanedo-Cázares
authors.forename: Cornelia
authors.initials: C
authors.lastname: Fuentes-Ahumada
authors.forename: Cuauhtémoc
authors.initials: C
authors.lastname: Oros-Ovalle
authors.forename: Josefina
authors.initials: J
authors.lastname: Navarrete-Solis
authors.forename: Benjamin
authors.initials: B
authors.lastname: Moncada
mesh_terms: D000328:Adult; D001485:Basement Membrane / Q000473:pathology*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000473:pathology*; D008548:Melanosis / Q000378:metabolism* / Q000473:pathology; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D019089:Stem Cell Factor / Q000378:metabolism*; D053802:Tryptases / Q000378:metabolism
chemical_list: D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit; D053802:Tryptases