title: [Effect of low glucose and/or hypoxia on the proliferation and metabolism of neural stem cells].
abstract: OBJECTIVE
  Recent study demonstrated that hypoxia could regulate the proliferation and differentiation of neural stem cells in vitro. In the present study, effects of low glucose and/or hypoxia on the proliferation and metabolism of neural stem cells were investigated in vitro.
  
  
  METHODS
  The neural stem cells were isolated from the rat embryonic mesencephalon (E13.5), and exposed to different oxygen concentrations (low oxygen: 3% O2 or normoxia: 20% O2) and different glucose concentrations (high glucose concentration: 4.5 g/L and low glucose concentration: 1.4 g/L) for 3 days. The proliferation of neural stem cells were examined by CCK-8 assay. Furthermore, the content of glucose, lactate, and pyruvic acid in the medium were measured after cultured in different condition for 1, 3, 5 days.
  
  
  RESULTS
  Low oxygen and low glucose could increase the proliferation of neural stem cells respectively; in addition, the number of neurospheres under both low oxygen and glucose was the most among the four groups. The content of glucose and pyruvic acid in the medium from low oxygen or low glucose condition decreased, while the lactate concentration increased compared with the control group.
  
  
  CONCLUSIONS
  The results indicate the neural stem cells prefer grow under the low glucose and low oxygen condition, and that is mainly under going glycolysis to maintain its self-renew ability. This study may provide us a useful clue for application of neural stem cells transplantation.
authors.affiliation: Department of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Xiong
authors.forename: Kuan
authors.initials: K
authors.lastname: Zhang
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Kui-Wu
authors.initials: KW
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005947:Glucose