title: Potassium channel expression in adult murine neural progenitor cells.
abstract: Neural progenitor cells (NPCs) are a source of new neurons and glia in the adult brain. Most NPCs reside in the forebrain subventricular zone (SVZ) and in the subgranular zone of the dentate gyrus, where they contribute to plasticity in the adult brain. To use their potential for repair, it is essential to identify the molecules that regulate their growth, migration and differentiation. Potassium (K+) channels are promising molecule candidates for NPC regulation as they are important components of signal transduction and their diversity is ideal to cover the complex functions required for cell proliferation and differentiation. There is increasing evidence that K+ channels influence cell growth and neurogenesis, however, very little is known regarding K+ channel distribution in NPCs. We therefore explored the expression of a variety of voltage-gated (Kv), inwardly rectifying (Kir) and two-pore (K2P) K+ channels in the SVZ of adult mice and in neurosphere cultures of NPCs during growth and differentiation. Immunocytochemical analysis revealed a differential expression pattern of K+ channels in nestin+ SVZ precursor cells, early SVZ doublecortin+ neurons and (sub)ependymal cells. These findings were confirmed in neurosphere cultures at the protein and mRNA levels. The expression of some K+ channel proteins, such as Kir4.1, Kir6.1, TREK1 or TASK1, suggests a role of K+ channels in the complex regulation of NPC proliferation, maturation and differentiation.
authors.affiliation: Department of Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité-Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin. harald.pruess@charite.de
authors.forename: H
authors.initials: H
authors.lastname: Prüss
authors.forename: M
authors.initials: M
authors.lastname: Dewes
authors.forename: C
authors.initials: C
authors.lastname: Derst
authors.forename: F
authors.initials: F
authors.lastname: Fernández-Klett
authors.forename: R W
authors.initials: RW
authors.lastname: Veh
authors.forename: J
authors.initials: J
authors.lastname: Priller
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015221:Potassium Channels / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D015221:Potassium Channels