title: Genomic sequence of temperate phage TEM126 isolated from wild type S. aureus.
abstract: Bacteriophage TEM126, a newly isolated temperate phage from a mitomycin-C-induced lysate of wild-type Staphylococcus aureus isolated from food, has an isometric head, a noncontractile tail, and a double-stranded DNA genome with a length of 33,540 bp and a G+C content of 33.94%. Bioinformatics analysis of the phage genome revealed 44 putative open reading frames (ORFs). Predicted protein products of the ORFs were determined and described. Temperate phage TEM126 can be classified as a member of the family Siphoviridae by morphology and genome structure. Temperate phage TEM126 showed 84% similarity with Staphylococcus phage phiNM1. To our knowledge, this is the first report of genomic sequencing and characterization of temperate phage TEM126 from a wild-type S. aureus isolated from foods in Korea.
authors.affiliation: School of Life Science and Biotechnology, Korea University, Anam-Dong, Sungbuk-Gu, Seoul, South Korea.
authors.forename: Young-Duck
authors.initials: YD
authors.lastname: Lee
authors.forename: Hyo-Ihl
authors.initials: HI
authors.lastname: Chang
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Park
mesh_terms: D001482:Base Composition; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D005516:Food Microbiology; D016679:Genome, Viral; D008242:Lysogeny; D016685:Mitomycin / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D039002:Prophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013204:Staphylococcus Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000821:virology*; D014771:Virion / Q000648:ultrastructure; D014775:Virus Activation / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D016685:Mitomycin; D004247:DNA