title: Fabrication of DNA polymer brush arrays by destructive micropatterning and rolling-circle amplification.
abstract: A method for fabricating DNA polymer brush arrays using photolithography and plasma etching followed by solid-phase enzymatic DNA amplification is reported. After attaching oligonucleotide primers to the surface of a glass coverslip, a thin layer of photoresist is spin-coated on the glass and patterned via photolithography to generate an array of posts in the resist. An oxygen-based plasma is then used to destroy the exposed oligonucleotide primers. The glass coverslip with the primer array is assembled into a microfluidic chip and DNA polymer brushes are synthesized on the oligonucleotide array by rolling-circle DNA amplification. We have demonstrated that the linear polymers can be rapidly synthesized in situ with a high degree of control over their density and length.
authors.affiliation: Department of Bioengineering, University of California San Diego, La Jolla, CA 92093-0412, USA.
authors.forename: Kristopher D
authors.initials: KD
authors.lastname: Barbee
authors.forename: Matt
authors.initials: M
authors.lastname: Chandrangsu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Huang
mesh_terms: D001483:Base Sequence; D004270:DNA, Circular / Q000737:chemistry*; D005898:Glass; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055616:Microtechnology; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D011089:Polydeoxyribonucleotides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D004270:DNA, Circular; D011089:Polydeoxyribonucleotides; D011092:Polyethylene Glycols