title: CYP1A mRNA expression in redeye mullets (Liza haematocheila) from Bohai Bay, China.
abstract: Induction of cytochrome P4501A (CYP1A) has been used as a biomarker in fish for monitoring aromatic and organic contaminants. In this study, a partial of CYP1A gene in redeye mullet (Liza haematocheila) was isolated and sequenced, and then a real-time quantitative reverse-transcription polymerase chain reaction assay was developed for quantification of CYP1A mRNA normalized to β-actin. The developed method was applied to detect CYP1A mRNA expression in redeye mullets collected from Nandaihe (reference site) and Dashentang (impacted site) in Bohai Bay, China. CYP1A mRNA expression values were significantly elevated in redeye mullets from Dashentang compared to a reference site--Nandaihe, which was correlated with the contents of different environmentally relevant pollutants in tissues, particularly with PCBs and PBDEs.
authors.affiliation: MOE Laboratory of Earth Surface Processes, College of Urban and Environmental Science, Peking University, Beijing 100871, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: An
authors.forename: Jianying
authors.initials: J
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Binghui
authors.initials: B
authors.lastname: Zheng
authors.forename: An
authors.initials: A
authors.lastname: Wei
authors.forename: Jingjing
authors.initials: J
authors.lastname: Shang
authors.forename: Xingru
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002681:China; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D004784:Environmental Monitoring; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism / Q000633:toxicity; D008969:Molecular Sequence Data; D011078:Polychlorinated Biphenyls / Q000378:metabolism / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism; D023701:Smegmamorpha / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D055768:Halogenated Diphenyl Ethers; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1