title: Liquid chromatography and mass spectrometry in food allergen detection.
abstract: Food allergy is an important issue in the field of food safety because of the hazards for affected persons and the hygiene requirements and legal regulations imposed on the food industry. Consumer protection and law enforcement require suitable analytical techniques for the detection of allergens in foods. Immunological methods are currently preferred; however, confirmatory alternatives are needed. The determination of allergenic proteins by liquid chromatography and mass spectrometry has greatly advanced in recent years, and gel-free allergenomics is becoming a routinely used approach for the identification and quantitation of food allergens. The present review provides a brief overview of the principles of proteomic procedures, various chromatographic set ups, and mass spectrometry instrumentation used in allergenomics. A compendium of published liquid chromatography methods, proteomic analyses, typical marker peptides, and quantitative assays for 14 main allergy-causing foods is also included.
authors.affiliation: Section of Chemistry, Department of Feed and Food Safety, National Veterinary Institute, P.O. Box 750 Sentrum, Oslo N-0106, Norway. christiane.faste@vetinst.no
authors.forename: Christiane Kruse
authors.initials: CK
authors.lastname: Fæste
authors.forename: Helene Thorsen
authors.initials: HT
authors.lastname: Rønning
authors.forename: Uwe
authors.initials: U
authors.lastname: Christians
authors.forename: Per Einar
authors.initials: PE
authors.lastname: Granum
mesh_terms: D000485:Allergens / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D003257:Consumer Product Safety; D005504:Food Analysis; D005512:Food Hypersensitivity / Q000517:prevention & control; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics
chemical_list: D000485:Allergens