title: A unified platform for optoelectrowetting and optoelectronic tweezers.
abstract: A platform capable of seamlessly unifying both optoelectrowetting and optoelectronic tweezers is presented. This enables the user to manipulate aqueous droplets (with electrowetting) as well as individual particles within those droplets (with dielectrophoresis). The device requires no photolithography and droplet/particle manipulation can occur continuously over the entire surface of the device. Droplet and 10 µm polystyrene particle speeds of up to 8 mm s(-1) and 60 µm s(-1), respectively, are demonstrated. Particle concentration within, and subsequent splitting of, a droplet is performed resulting in average concentration efficiencies of 93%. Serial concentration is also demonstrated resulting in exponentially increasing particle concentrations and a 10× concentration increase. Finally, the platform is used to select a single cell out of a cohort and subsequently encapsulate it in its own aqueous droplet.
authors.affiliation: Berkeley Sensor and Actuator Center, Department of Electrical Engineering and Computer Science, University of California Berkeley, 497 Cory Hall, Berkeley, CA 94720, USA. valleyj@eecs.berkeley.edu.
authors.forename: Justin K
authors.initials: JK
authors.lastname: Valley
authors.forename: Shao Ning
authors.initials: SN
authors.lastname: Pei
authors.forename: Shao
authors.initials: S
authors.lastname: Ningpei
authors.forename: Arash
authors.initials: A
authors.lastname: Jamshidi
authors.forename: Hsan-Yin
authors.initials: HY
authors.lastname: Hsu
authors.forename: Ming C
authors.initials: MC
authors.lastname: Wu
mesh_terms: D002469:Cell Separation; D058874:Electrowetting / Q000295:instrumentation / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D008839:Microelectrodes; D055620:Optical Phenomena; D011327:Printing; D013499:Surface Properties; D013997:Time Factors