title: Immobilizing Pt nanoparticles and chitosan hybrid film on polyaniline naofibers membrane for an amperometric hydrogen peroxide biosensor.
abstract: A convenient and effective way for fabricating amperometric hydrogen peroxide (H2O2) biosensor was designed in this paper. First, the polyaniline (PANI) nanofibers membrane with good conductance and high surface area was electropolymerized on a gold electrode surface. Then, Pt nanoparticle (PtNP) was electrochemically deposited on the PANI nanofibers membrane. Finally, the hybrid film of gold nanoparticle, chitosan, and horseradish peroxidase (HRP) was cast onto the modified electrode to form a stable biofunctional film, which was also employed as a protective layer to PtNP. The proposed biosensor exhibited a rapid response to H2O2 with the linear range from 7.0 × 10(-6) to 1.4 × 10(-2) M and a detection limit of 2.8 × 10(-6) M (S/N = 3). The sensitivity of 558 μA mM(-1) cm(-2) was obtained. The Michaelis-Menten constant, K(app)M value was 1.90 mM suggesting a high affinity. Moreover, it displayed a good reproducibility and long-term stability.
authors.affiliation: Key Laboratory of Chongqing Modern Analytical Chemistry, College of Chemistry and Engineering, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Shihong
authors.initials: S
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
authors.forename: Bing
authors.initials: B
authors.lastname: Yin
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes; D004800:Enzymes, Immobilized; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; C416807:polyaniline; D010984:Platinum; D006046:Gold; D048271:Chitosan; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase