title: Redistribution of meta-tetra(hydroxyphenyl)chlorin (m-THPC) from conventional and PEGylated liposomes to biological substrates.
abstract: We used the phenomenon of previously described photoinduced fluorescence quenching and fluorescence polarization to evaluate the transfer of meta-tetra(hydroxyphenyl)chlorin (m-THPC) from commercial high-drug load liposomes to plasma proteins and model membranes. Fluorescence quenching of m-THPC in liposomes by iodide indicates that part of m-THPC in PEGylated liposomes is localized in the PEG shell, while the rest is bound to the lipid bilayer. It was shown that the two molecule pools in the commercial PEGylated liposomal formulation Fospeg® condition the characteristics of the m-THPC release kinetics. A substantial percentage of m-THPC from Fospeg® is released much faster than from the conventional liposomal formulation Foslip®. Using the technique of resonance light scattering, it was shown that partial m-THPC aggregation is present in liposomes with very high drug loads, higher in PEGylated liposomes compared to conventional ones.
authors.affiliation: Centre de Recherche en Automatique de Nancy, Nancy-Universite, CNRS, Centre Alexis Vautrin, Vandoevre-les-Nancy, France.
authors.forename: Vadzim
authors.initials: V
authors.lastname: Reshetov
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Kachatkou
authors.forename: Tatiana
authors.initials: T
authors.lastname: Shmigol
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zorin
authors.forename: Marie-Ange
authors.initials: MA
authors.lastname: D'Hallewin
authors.forename: Francois
authors.initials: F
authors.lastname: Guillemin
authors.forename: Lina
authors.initials: L
authors.lastname: Bezdetnaya
mesh_terms: D001798:Blood Proteins / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D006801:Humans; D007700:Kinetics; D008027:Light; D008081:Liposomes; D008652:Mesoporphyrins / Q000737:chemistry*; D017319:Photosensitizing Agents / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012542:Scattering, Radiation; D013696:Temperature; D013816:Thermodynamics
chemical_list: D001798:Blood Proteins; D008081:Liposomes; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D011092:Polyethylene Glycols; C072269:temoporfin