title: Composition of the bacterial biota in slime developed in two machines at a Canadian paper mill.
abstract: During the process of papermaking by pulp and paper plants, a thick and viscous deposits, termed slime, is quickly formed around the paper machines, which can affect the papermaking process. In this study, we explored the composition of the bacterial biota in slime that developed on shower pipes from 2 machines at a Canadian paper mill. Firstly, the composition was assessed for 12 months by DNA profiling with polymerase chain reaction coupled with denaturing gradient gel electrophoresis. Except for short periods (2-3 months), clustered analyses showed that the bacterial composition of the slime varied substantially over the year, with less than 50% similarity between the denaturing gradient gel electrophoresis profiles. Secondly, the screening of 16S rRNA gene libraries derived from 2 slime samples showed that the most abundant bacteria were related to 6 lineages, including Chloroflexi, candidate division OP10, Clostridiales, Bacillales, Burkholderiales, and the genus Deinococcus. Finally, the proportion of 8 bacterial lineages, such as Deinococcus sp., Meiothermus sp., and Chloroflexi, was determined by the Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization in 2 slime samples. The results showed a high proportion of Chloroflexi, Tepidimonas spp., and Schlegelella spp. in the slime samples.
authors.affiliation: INRS-Institut Armand-Frappier, 531 boulevard des Prairies, Laval, QC H7V 1B7, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Disnard
authors.forename: Carole
authors.initials: C
authors.lastname: Beaulieu
authors.forename: Richard
authors.initials: R
authors.lastname: Villemur
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D018441:Biofilms; D057219:Biofouling; D058448:Biota; D002170:Canada; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D007220:Industrial Waste; D010209:Paper; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S