title: Suppression of mast-cell-mediated allergic inflammation by Lindera obtusiloba.
abstract: Allergic disease is a consequence of exposure to normally innocuous substances that elicit the activation of mast cells. Mast-cell-mediated allergic response is involved in many diseases such as anaphylaxis, allergic rhinitis, asthma and atopic dermatitis. The discovery of drugs for the treatment of allergic disease is an important subject in human health. In this study, we investigated the effect of Lindera obtusiloba water extract (LOWE) on the mast-cell-mediated allergic inflammation and possible mechanism of action using in vitro and in vivo models. LOWE reduced histamine release from various types of mast cells activated by immunoglobulin E (IgE) or phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI). The inhibitory effect of LOWE on histamine release was mediated by calcium signal. LOWE decreased the PMACI-stimulated gene expression of proinflammatory cytokines such as tumor necrosis factor-α and interleukin-6 in human mast cells. The inhibitory effect of LOWE on the proinflammatory cytokines was nuclear factor (NF)-κB dependent. In addition, LOWE suppressed compound 48/80-induced systemic allergic reaction and serum histamine release in mice and IgE-mediated local allergic reactions. Our results indicate that LOWE inhibits mast-cell-derived allergic inflammation and involvement of calcium, histamine, proinflammatory cytokines and NF-κB in these effects.
authors.affiliation: College of Pharmacy, Youngnam University, Kyungsan 712-749, South Korea.
authors.forename: Won Mo
authors.initials: WM
authors.lastname: Suh
authors.forename: Seung-Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Soyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Hui-Hun
authors.initials: HH
authors.lastname: Kim
authors.forename: Kyoungho
authors.initials: K
authors.lastname: Suk
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Son
authors.forename: Taeg Kyu
authors.initials: TK
authors.lastname: Kwon
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Choi
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004195:Disease Models, Animal; D015870:Gene Expression; D006632:Histamine / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy*; D007249:Inflammation / Q000517:prevention & control*; D015850:Interleukin-6 / Q000096:biosynthesis; D027443:Lindera / Q000737:chemistry*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification / Q000494:pharmacology; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000093242:Interleukin-6 Inhibitors
chemical_list: D018926:Anti-Allergic Agents; D002118:Calcium; D006632:Histamine; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors