title: Comprehensive study of alkaloids from Crinum asiaticum var. sinicum assisted by HPLC-DAD-SPE-NMR.
abstract: A comprehensive study of the alkaloids presentin the leaves of Crinum asiaticum var. sinicum, assisted by HPLC-SPE-NMR, led to the characterization of 21 compounds of similar polarity on an analytical scale. Thirteen of these were isolated for further structural confirmation. Seven are proved to be new, namely, (+)-siculine (4), 1-epijosephinine (11), 7-methoxycrinamabine (10), 2-O-acetylcrinamabine (16), 3-O-acetyl-8-O-demethylmaritidine (17), 2-O-acetylbulbisine (18), and 1-O-acetylbulbisine (19). In addition, dihydrovittatine (6) and 8-O-demethyloxomaritidine (21) were isolated for the first time from Nature, although they have been prepared previously as synthetic products. Their structures were established by spectroscopic analysis.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, 1, Sec. 1, Jen-Ai Rd, Taipei 10051, Taiwan, Republic of China.
authors.forename: Chien-Kuang
authors.initials: CK
authors.lastname: Chen
authors.forename: Feng-Hsu
authors.initials: FH
authors.lastname: Lin
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Tseng
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Jiang
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D031407:Crinum / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000470:Alkaloids