title: [Cisplatin-induced apoptosis in human lung adenocarcinoma cell line A549].
abstract: BACKGROUND
  To elucidate the pattern and mechanism of cisplatin-induced apoptosis and its role in tumor chemotherapy.
  
  
  METHODS
  Apoptosis induced by cisplatin in human lung adenocarcinoma cell line A549 was detected by cell morphology, agarose gel electrophoresis, DNA-end-labeling and flow cytometry analysis techniques.
  
  
  RESULTS
  Cisplatin-induced apoptosis of A549 cells persisted and augmented gradually from 12 to 72 hours after treated with 3 mg/L cisplatin. All of A549 cells treated respectively with 1, 3, 5 and 7 mg/L cisplatin showed apoptosis. Apoptotic effects increased in a time-dependent pattern and a concentration-dependent pattern. A549 cells were blocked in G1 phase after treated with cisplatin.
  
  
  CONCLUSIONS
  Induction of cell apoptosis may be an important mechanism of anti-tumor efficacy of cisplatin.
authors.affiliation: Department of Oncology, Guangdong 177 Hospital, Guangzhou, Guangdong 510317, P.R.China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Liu
authors.forename: Jinhan
authors.initials: J
authors.lastname: Li
authors.forename: Yide
authors.initials: Y
authors.lastname: Hu
authors.forename: Guisheng
authors.initials: G
authors.lastname: Qian