title: ICP27 protein of Marek's disease virus interacts with SR proteins and inhibits the splicing of cellular telomerase chTERT and viral vIL8 transcripts.
abstract: All herpesviruses have a post-transcriptional regulatory protein that prevents precursor mRNA splicing and leads to the shutting off of host protein synthesis. The ICP27 protein of herpes simplex virus 1 (HSV-1) is the prototype of these proteins. Marek's disease virus (MDV-1), an alphaherpesvirus that induces lymphoma in birds, also has an ICP27 protein that is produced in lytic MDV-1-infected cells. We characterized this protein. We demonstrated ICP27 production in latently infected MSB-1 cells, but only on MDV-1 reactivation. ICP27 was found predominantly in specific structures within the nucleus. The ICP27 of MDV-1 colocalized and interacted with SR proteins. We demonstrated inhibitory effects of MDV-1 ICP27 on the splicing of both the viral vIL8 and cellular chTERT (telomerase reverse transcriptase) genes. Thus, the ICP27 of MDV-1 plays a similar role to the ICP27 of HSV-1 and may be involved in MDV-1 replication and the development of Marek's disease.
authors.affiliation: Equipe TLVI, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc de Grandmont, 37200 Tours, France.
authors.forename: S
authors.initials: S
authors.lastname: Amor
authors.affiliation: Equipe TLVI, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc de Grandmont, 37200 Tours, France.
authors.forename: S
authors.initials: S
authors.lastname: Strassheim
authors.affiliation: INRA, Département de Santé Animale, Centre de recherches de Tours, 37380 Nouzilly, France.|Equipe TLVI, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc de Grandmont, 37200 Tours, France.
authors.forename: G
authors.initials: G
authors.lastname: Dambrine
authors.affiliation: INRA, Département de Santé Animale, Centre de recherches de Tours, 37380 Nouzilly, France.
authors.forename: S
authors.initials: S
authors.lastname: Remy
authors.affiliation: Equipe TLVI, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc de Grandmont, 37200 Tours, France.
authors.forename: D
authors.initials: D
authors.lastname: Rasschaert
authors.affiliation: INRA, Département de Santé Animale, Centre de recherches de Tours, 37380 Nouzilly, France.|Equipe TLVI, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc de Grandmont, 37200 Tours, France.
authors.forename: S
authors.initials: S
authors.lastname: Laurent
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002645:Chickens; D015536:Down-Regulation; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D008380:Marek Disease / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D008969:Molecular Sequence Data; D011485:Protein Binding; D021381:Protein Transport; D012326:RNA Splicing; D017381:Spliceosomes / Q000235:genetics / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C089456:ICP27 protein, Marek's disease virus; D016209:Interleukin-8; D014764:Viral Proteins; D019098:Telomerase