title: Proof of concept for recombinant cellular controls in quantitative molecular pathogen detection.
abstract: In this study, we present the concept of internal sample process controls (ISPCs) to monitor the efficiency of an analytical chain using sample preparation and quantitative PCR (qPCR). A recombinant Listeria monocytogenes ΔprfA (targeted deletion) strain containing a competitive artificial single-copy genomic target was applied to naturally contaminated samples to demonstrate its analytical suitability as an ISPC.
authors.affiliation: Christian Doppler Laboratory for Molecular Food Analytics, Veterinärplatz 1, 1210 Vienna, Austria. peter.rossmanith@vetmeduni.ac.at
authors.forename: Peter
authors.initials: P
authors.lastname: Rossmanith
authors.forename: Patrick
authors.initials: P
authors.lastname: Mester
authors.forename: Karin
authors.initials: K
authors.lastname: Frühwirth
authors.forename: Sabine
authors.initials: S
authors.lastname: Fuchs
authors.forename: Martin
authors.initials: M
authors.lastname: Wagner
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards*; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification; D008088:Listeriosis / Q000175:diagnosis; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D011786:Quality Control