title: The myocyte expression of adiponectin receptors and PPARδ is highly coordinated and reflects lipid metabolism of the human donors.
abstract: Muscle lipid oxidation is stimulated by peroxisome proliferator-activated receptor (PPAR) δ or adiponectin receptor signalling. We studied human myocyte expression of the PPARδ and adiponectin receptor genes and their relationship to lipid parameters of the donors. The mRNA levels of the three adiponectin receptors, AdipoR1, AdipoR2, and T-cadherin, were highly interrelated (r ≥ 0.91). However, they were not associated with GPBAR1, an unrelated membrane receptor. In addition, the adiponectin receptors were positively associated with PPARδ expression (r ≥ 0.75). However, they were not associated with PPARα. Using stepwise multiple linear regression analysis, PPARδ was a significant determinant of T-cadherin (P = .0002). However, pharmacological PPARδ activation did not increase T-cadherin expression. The myocyte expression levels of AdipoR1 and T-cadherin were inversely associated with the donors' fasting plasma triglycerides (P < .03). In conclusion, myocyte expression of PPARδ and the adiponectin receptors are highly coordinated, and this might be of relevance for human lipid metabolism in vivo.
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, Eberhard Karls University Tübingen and German Center for Diabetes Research (DZD eV), Tübingen, Germany.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Ordelheide
authors.forename: Martin
authors.initials: M
authors.lastname: Heni
authors.forename: Nadja
authors.initials: N
authors.lastname: Gommer
authors.forename: Lisa
authors.initials: L
authors.lastname: Gasse
authors.forename: Carina
authors.initials: C
authors.lastname: Haas
authors.forename: Alke
authors.initials: A
authors.lastname: Guirguis
authors.forename: Fausto
authors.initials: F
authors.lastname: Machicao
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Häring
authors.forename: Harald
authors.initials: H
authors.lastname: Staiger
mesh_terms: D000328:Adult; D015820:Cadherins / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D006801:Humans; D016014:Linear Models; D050356:Lipid Metabolism; D008297:Male; D032342:Muscle Cells / Q000737:chemistry / Q000378:metabolism*; D047494:PPAR delta / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000032:analysis; D054419:Receptors, Adiponectin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014280:Triglycerides / Q000097:blood
chemical_list: C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D015820:Cadherins; C099972:H-cadherin; D047494:PPAR delta; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D014280:Triglycerides