title: Synthetic glucocorticoids in the environment: first results on their potential impacts on fish.
abstract: Human pharmaceuticals have been shown to be entering the aquatic environment in quantities that may produce adverse effects to aquatic organisms. This paper investigates the impacts of synthetic glucocorticoids (GCs), which are used in large amounts as anti-inflammatory drugs, on fish. Mammalian cell lines were transiently transfected with trout corticosteroid receptors (GR1, GR2, and MR) and the transactivation abilities of ten of the most prescribed GCs in the UK were measured in vitro. They showed significantly higher activity with GR2 than with GR1. In order to assess any impacts in vivo, adult fathead minnows were exposed to either 1 μg prednisolone/L or 1 μg beclomethasone dipropionate/L for 21 days. Plasma glucose concentrations were increased and leucocytes were reduced significantly in GC-exposed groups compared to the control group. In another experiment, fish were exposed to three different concentrations of Beclomethasone dipropionate and a dose-dependent increase of plasma glucose was found. The results suggest that low concentrations of synthetic GCs present in water could cause adverse effects on fish. Therefore, quantification of GCs in the aquatic environment and the effects of GCs at environmentally relevant concentrations are required in order to determine if GCs pose a threat to wild fish populations.
authors.affiliation: Institute for the Environment, Brunel University, Uxbridge, Middlesex UB8 3PH UK. Kugathas.Subramaniam@brunel.ac.uk
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Kugathas
authors.forename: John P
authors.initials: JP
authors.lastname: Sumpter
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000633:toxicity*; D001507:Beclomethasone / Q000633:toxicity; D019556:COS Cells; D002522:Chlorocebus aethiops; D003530:Cyprinidae / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005938:Glucocorticoids / Q000633:toxicity*; D011239:Prednisolone / Q000633:toxicity; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014337:Trout / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D014874:Water Pollutants, Chemical; D011239:Prednisolone; D001507:Beclomethasone