title: HPTLC densitometric analysis of arbutin in bulk drug and methanolic extracts of Arctostaphylos uva-ursi.
abstract: A high-performance thin layer chromatographic densitometric method for the analysis of arbutin was developed and validated in the present investigation. Arbutin was separated on aluminium-backed silica gel 60 F(254) plates with methanol : chloroform (3:7)% (v/v) as the mobile phase. This system was found to give a compact spot of arbutin at a retention factor (R(f)) value of 0.32 ± 0.02. The limit of detection and limit of quantification were found to be 35.42 and 106.26 ng/spot, respectively. The proposed method with a high degree of precision and accuracy was employed for the analysis of arbutin in the bulk drug and methanolic extract of Arctostaphylos uva-ursi.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Al-Kharj University, Al-Kharj, Kingdom of Saudi Arabia. prawez_pharma@yahoo.com
authors.forename: P
authors.initials: P
authors.lastname: Alam
authors.forename: S I
authors.initials: SI
authors.lastname: Alqasoumi
authors.forename: F
authors.initials: F
authors.lastname: Shakeel
authors.forename: M S
authors.initials: MS
authors.lastname: Abdel-Kader
mesh_terms: D001104:Arbutin / Q000032:analysis*; D029792:Arctostaphylos / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D016014:Linear Models; D000432:Methanol; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D001104:Arbutin; D000432:Methanol