title: [The sedative and hypnotic effects and safety of oral emulsified isoflurane in rats].
abstract: OBJECTIVE
  To investigate the sedative and hypnotic effects and safety of oral emulsified isoflurane in rats.
  
  
  METHODS
  Thirty healthy Sprague-Dawley (SD) rats were randomly divided into three groups. The rats in the emulsified isoflurane group were orally administered with emulsified isoflurane with the sequential method, while the rats in the normal sodium group and the intralipid group were orally administered with sodium and intralipid respectively. The rats in the emulsified isoflurane group received 2.15 mL/100 g of 8% emulsified isoflurane, a dosage calculated with an increase of a common ratio (r = 0.8) on the basis of median effective dose (ED50). Similarly, 2.15 mL/100 g of normal sodium and 2.15 mL/100 g of 30% intralipid were given to the rats in the normal sodium group and the intralipid group, respectively. The neurobehaviors of the rats were assessed, and the interval of disappearance and recovery of the righting reflex were recorded.
  
  
  RESULTS
  Oral emulsified isoflurane reduced the autonomic activity time and induced the loss of righting reflex. The ED50 of the loss of righting reflex was 1.72 mL/100 g. The rats orally administered with 2.15 mL/100 g of 8% emulsified isoflurane had 90% of loss of righting reflex, with a sleeping latency of 15-20 min and up to 60 min of sleeping duration.
  
  
  CONCLUSIONS
  Oral emulsified isoflurane has obvious sedative and hypnotic effects.
authors.affiliation: Department of Pharamcology & Biopharmaceutic, West China School of Pharamcy, Sichuan University, Chengdu 610041, China.
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Lin
authors.forename: Nan-fu
authors.initials: NF
authors.lastname: Luo
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D045703:Emulsifying Agents / Q000008:administration & dosage / Q000494:pharmacology; D006993:Hypnotics and Sedatives / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D007530:Isoflurane / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D045703:Emulsifying Agents; D006993:Hypnotics and Sedatives; D007530:Isoflurane