title: Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France).
abstract: Genotoxic effects of a polymetallic pollution gradient displayed by the Lot River and one of its tributary have been assessed on zebrafish Danio rerio. Three methods were compared: RAPD-PCR, the comet assay, and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) formation. The fishes were exposed for 14 days to waters collected from three stations: Joanis, a site polluted by cadmium (Cd) and zinc (Zn) (mean concentrations: 15 μg Cd/L and 550 μg Zn/L), Bouillac (mean concentrations: 0.55 μg Cd/L and 80 μg Zn/L), and Boisse-Penchot, a reference station (mean concentrations: <0.05 μg Cd/L and 7 μg Zn/L). The quantitative RAPD-PCR methodology proved to be sensitive enough to unmask metal genotoxicity after 3 and 7 days of exposure to Joanis water and after 14 days to Bouillac water, whereas the comet assay only detected DNA damages at the most contaminated station (Joanis). The 8-oxodG quantification was not sensitive enough to be used in zebrafish under these environmental conditions.
authors.affiliation: Université de Bordeaux-CNRS, UMR EPOC 5805, Arcachon Marine Station, Place du Dr Peyneau, 33120 Arcachon, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Orieux
authors.forename: Sébastien
authors.initials: S
authors.lastname: Cambier
authors.forename: Patrice
authors.initials: P
authors.lastname: Gonzalez
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Morin
authors.forename: Christelle
authors.initials: C
authors.lastname: Adam
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Garnier-Laplace
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bourdineaud
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004784:Environmental Monitoring; D005602:France; D015870:Gene Expression / Q000187:drug effects; D009153:Mutagens / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism*; D015032:Zinc / Q000032:analysis / Q000378:metabolism / Q000633:toxicity
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical; D002104:Cadmium; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D015032:Zinc