title: An approach to speed up the isolation of hydrophilic metabolites from natural sources at semipreparative level by using a hydrophilic-lipophilic balance/mixed-mode strong cation exchange-high-performance liquid chromatography/mass spectrometry system.
abstract: An approach to speed up the isolation of hydrophilic metabolites in complex natural matrixes by using a HLB/MCX-HPLC/MS system based on the retention properties of hydrophilic-lipophilic and cation exchange polymeric cartridges was developed. This methodology was successfully applied to the re-isolation of small water soluble compounds with completely different structures from two different natural extracts such as a dipeptide (vanchrobactin) from a bacterium culture broth and a pyrrolidine bearing a carboxylic acid moiety (clionapyrrolidine A) from a sponge. This method improved not only the efficiency of the isolation methodology but also the isolation time in relation to the existing methods.
authors.affiliation: Analytical Technologies DCR&T Alcobendas, Lilly S.A., Avenida de la Industria 30, Alcobendas/Madrid, Spain. espada_alfonso@lilly.com
authors.forename: Alfonso
authors.initials: A
authors.lastname: Espada
authors.forename: Cristina
authors.initials: C
authors.lastname: Anta
authors.forename: Aroa
authors.initials: A
authors.lastname: Bragado
authors.forename: Jaime
authors.initials: J
authors.lastname: Rodríguez
authors.forename: Carlos
authors.initials: C
authors.lastname: Jiménez
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification; D011161:Porifera; D011759:Pyrrolidines / Q000302:isolation & purification; D017262:Siderophores / Q000302:isolation & purification*; D014733:Vibrio; D014867:Water
chemical_list: D002412:Cations; D045424:Complex Mixtures; D010455:Peptides; D011759:Pyrrolidines; D017262:Siderophores; C538947:clionapyrrolidine A; C529766:vanchrobactin; D014867:Water