title: Full length antigen priming enhances the CTL epitope-based DNA vaccine efficacy.
abstract: Although CD8+ cytotoxic T lymphocyte (CTL) epitope-based DNA vaccination is valuable experience on vaccine research but many attempts are still continued to achieve acceptable protective response. To study the role of full length antigen in CTL epitope immunization, we evaluated cellular immunity of diverse patterns of complete Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) and the immunodominant CTL epitope (498-505) DNA injection in C57BL/6 mice. Optimal immune response was observed in the group immunized with the full length of gB in the first injection and CTL epitope in the second and third vaccination as assessed by lymphocyte proliferation assay (MTT), cytokine assay (ELISA) and CTL assay. B cell and spatially CD4+ T cell epitopes in full length protein might be important for appropriate priming of CTL immune response. These findings may have important implication for the improvement of CTL epitope based DNA vaccine against HSV and other pathogens.
authors.affiliation: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
authors.forename: Mohammad Hassan
authors.initials: MH
authors.lastname: Pouriayevali
authors.forename: Taravat
authors.initials: T
authors.lastname: Bamdad
authors.forename: Masoud
authors.initials: M
authors.lastname: Parsania
authors.forename: Rohollah Dorostkar
authors.initials: RD
authors.lastname: Sari
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D016207:Cytokines / Q000097:blood; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000235:genetics; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C040495:glycoprotein B, Simplexvirus