title: Evaluation of a commercial Dengue NS1 enzyme-linked immunosorbent assay for early diagnosis of dengue infection.
abstract: OBJECTIVE
  Dengue is one of the most serious mosquito-borne viral infections affecting tropical and subtropical countries in the world. Since there is no immunoprophylactic or specific antiviral therapy available, timely and rapid diagnosis plays a vital role in patient management and implementation of control measures. This paper evaluates a commercially available NS1 antigen capture ELISA vis-a-vis SD bioline Dengue NS1 antigen test for early detection of dengue virus.
  
  
  METHODS
  To evaluate a commercial NS1 antigen detection kit vis-a-vis SD bioline Dengue NS1 antigen test, a total of 91 clinical samples were tested. Virological investigations with regard to dengue virus, viz. NS1 antigen capture ELISA (Panbio, Australia), SD bioline Dengue NS1 antigen test, RT-PCR and virus isolation were performed.
  
  
  RESULTS
  Out of 91 samples, 24 (26%) were positive by NS1 antigen capture ELISA, 15 (16%) by SD bioline Dengue NS1 antigen test and 11(12%) positive by RT-PCR analysis. The RT-PCR-positive samples were further subjected to virus isolation and resulted in three isolates. The results of the Panbio NS1 antigen capture ELISA, SD bioline Dengue NS1 antigen test, RT-PCR and virus isolation were correlated among themselves.
  
  
  CONCLUSIONS
  The present study comprehensively established the utility of NS1 antigen ELISA in early diagnosis of dengue infection.
authors.affiliation: Defence Research and Development Establishment, Gwalior-474 002, India. ambujshrivastava@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Shrivastava
authors.forename: P K
authors.initials: PK
authors.lastname: Dash
authors.forename: N K
authors.initials: NK
authors.lastname: Tripathi
authors.forename: A K
authors.initials: AK
authors.lastname: Sahni
authors.forename: N
authors.initials: N
authors.lastname: Gopalan
authors.forename: P V
authors.initials: PV
authors.lastname: Lakshmana Rao
mesh_terms: D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000097:blood*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000302:isolation & purification; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins / Q000097:blood; D014773:Virology / Q000379:methods*; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins