title: [Determination of residual solvents in 7-amino-3-chloro cephalosporanic acid by gas chromatography].
abstract: To develop a gas chromatography method for determination of residual solvents in 7-amino-3-chloro cephalosporanic acid (7-ACCA).
  
  
  
  The residual levels of acetone, methanol, dichloromethane, ethyl acetate, isobutanol, pyridine and toluene in 7-ACCA were measured by gas chromatography using Agilent INNOWAX capillary column (30 m × 0.32 mm,0.5 μm). The initial column temperature was 70° maintained for 6 min and then raised (10°C/min) to 160°C for 1 min. Nitrogen gas was used as carrier and FID as detector. The flow of carrier was 1.0 ml/min, the temperature of injection port and detector was 200°C and 250°C, respectively.
  
  
  
  The limits of detection for acetone, methanol, dichloromethane, ethyl acetate, isobutanol, pyridine, toluene in 7-ACCA were 2.5 μg/ml, 1.5 μg/ml, 15 μg/ml, 2.5 μg/ml, 2.5 μg/ml, 2.5 μg/ml and 11 μg/ml, respectively. Only acetone was detected in the sample, and was less than the limits of Ch.P.
  
  
  
  The method can effectively detect the residual solvents in 7-ACCA.
authors.affiliation: Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Tong-wei
authors.initials: TW
authors.lastname: Yao
mesh_terms: D000096:Acetone / Q000032:analysis; D002511:Cephalosporins; D002849:Chromatography, Gas / Q000379:methods*; D004340:Drug Contamination; D000432:Methanol / Q000032:analysis; D008752:Methylene Chloride / Q000032:analysis; D012997:Solvents / Q000032:analysis*; D014050:Toluene / Q000032:analysis
chemical_list: D002511:Cephalosporins; D012997:Solvents; D000096:Acetone; D014050:Toluene; D008752:Methylene Chloride; D000432:Methanol