title: DNA-directed assembly microarray for protein and small molecule inhibitor screening.
abstract: A robust high-throughput and high-fidelity screening platform for identifying and validating potential target molecules is the key for drug development. During the past decade, microarray platforms have demonstrated enormous potential for developing robust tools for small molecules as well as protein-based drug discovery and analysis. Recently, we developed a DNA-directed assembly microarray platform with improved screening and immobilization strategies. In contrast to conventional microarray platforms, our technique allows the solution phase interaction of the probes and analytes in a biological environment and further the detection through the directed assembly of specific DNA probes on a dendrimer-modified glass surface. Herein, we describe the detailed experimental protocols in performing the DNA-directed assembly platform for antibody microarray, a RNA polymerase-DNA binding microarray, and a drug-screening microarray.
authors.affiliation: Singapore-Massachusetts Institute of Technology Alliance (SMA), National University of Singapore, Singapore, Singapore.
authors.forename: Ng Jin
authors.initials: NJ
authors.lastname: Kiat
authors.forename: Fritz
authors.initials: F
authors.lastname: Simeon
authors.forename: Too Heng
authors.initials: TH
authors.lastname: Phon
authors.forename: Parayil Kumaran
authors.initials: PK
authors.lastname: Ajikumar
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000378:metabolism; D050091:Dendrimers / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D008969:Molecular Sequence Data; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000906:Antibodies; D004277:DNA, Single-Stranded; D050091:Dendrimers; C104700:PAMAM Starburst; D011506:Proteins; D012321:DNA-Directed RNA Polymerases