title: Long-term effects of intermittent equine parathyroid hormone fragment (ePTH-1-37) administration on bone metabolism in healthy horses.
abstract: Intermittent administration of parathyroid hormone (PTH) is an anabolic therapy for osteoporotic conditions in humans. This study evaluated the effects of equine PTH fragment (ePTH-1-37) administration on bone metabolism in 12 healthy horses. Six horses each were treated once daily for 120days with subcutaneous injections of 0.5μg/kg ePTH-1-37 or placebo. Blood was collected to determine ionized calcium (Ca(++)), total Ca (Ca(T)), inorganic phosphorus, serum equine osteocalcin (eOC), carboxy-terminal telopeptide of type I collagen (ICTP), bone-specific alkaline phosphatase, and carboxy-terminal cross-linked telopeptide of type I collagen. Bone mineral density (BMD) was determined with dual X-ray absorptiometry of the metacarpus and calcaneus. Significantly higher blood Ca(++) and plasma Ca(T) concentrations were measured 5h after ePTH-1-37 administration compared to placebo. Higher serum eOC concentrations were found for ePTH-1-37 treatment at days 90 (P<0.05) and 120 (P=0.05). Significantly higher serum ICTP levels were observed with ePTH-1-37 treatment at days 60 and 90. For both study groups, BMD increased significantly in the calcaneus. Long-term use of ePTH-1-37 seemed to have no negative effects on bone metabolism in healthy horses. The absence of undesirable side effects is the premise to ensure safety for further clinical investigations in horses with increased bone resorption processes.
authors.affiliation: Institute of Animal Nutrition, Nutrition Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Gustav-Kuehn-Str. 8, 04159 Leipzig, Germany; Functional Genomics and Bioregulation, Institute of Animal Genetics, FLI Mariensee, Hoeltystr. 10, 31535 Neustadt a. R., Germany.
authors.forename: Katharina U
authors.initials: KU
authors.lastname: Weisrock
authors.affiliation: Institute of Animal Nutrition, Nutrition Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Gustav-Kuehn-Str. 8, 04159 Leipzig, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Winkelsett
authors.affiliation: Department of Animal Sciences, INRA Center Clermont-Ferrand, 63122 Saint-Genès-Champanelle, France.
authors.forename: William
authors.initials: W
authors.lastname: Martin-Rosset
authors.affiliation: IPF Pharmaceuticals GmbH, Feodor-Lynen-Str. 31, 30625 Hannover, Germany.
authors.forename: Wolf-Georg
authors.initials: WG
authors.lastname: Forssmann
authors.affiliation: Functional Genomics and Bioregulation, Institute of Animal Genetics, FLI Mariensee, Hoeltystr. 10, 31535 Neustadt a. R., Germany.
authors.forename: Nahid
authors.initials: N
authors.lastname: Parvizi
authors.affiliation: Institute of Animal Nutrition, Nutrition Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Gustav-Kuehn-Str. 8, 04159 Leipzig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Coenen
authors.affiliation: Institute of Animal Nutrition, Nutrition Diseases and Dietetics, Faculty of Veterinary Medicine, University of Leipzig, Gustav-Kuehn-Str. 8, 04159 Leipzig, Germany. Electronic address: Ingrid.Vervuert@vetmed.uni-leipzig.de.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Vervuert
mesh_terms: D015502:Absorptiometry, Photon / Q000662:veterinary; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D004334:Drug Administration Schedule / Q000662:veterinary; D006736:Horses / Q000378:metabolism*; D007279:Injections, Subcutaneous / Q000662:veterinary; D008297:Male; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D010281:Parathyroid Hormone; D010446:Peptide Fragments