title: [TCRζ gene expression in TCR gene-modified T cells specific for DLBCL-associated antigen].
abstract: OBJECTIVE
  To analyze the expression level of TCRζ chain gene in the DLBCL-associated antigen-specific T cells before and after being activated by coculture with Toledo cells (DLBCL cell line).
  
  
  METHODS
  Real-time PCR with SYBR GreenI technique was used for detecting TCRζ chain expression in activated and unactivated DLBCL-associated antigen-specific T cells. β2 microglobulin gene (β2M) was used as an endogenous reference. Relative mRNA expression level of TCRζ gene was analyzed by the formula of both 2(-δCt); ×100% and 2(-δδCt);.
  
  
  RESULTS
  Compared with (1.74±0.28)% of the relative mRNA expression level of TCRζ gene in TCR gene-untransduced T cells, the expression level of TCRζ gene was (1.78±0.22)% in unactivated TCR gene-transduced T cells and showed no obvious increase. While the expression of TCRζ gene arrived at (11.54±1.98)% in the activated TCR gene-modified T cells, which was significantly higher than that in unactivated TCR gene-modified and TCR gene-untransduced T cells (P<0.05), and was increased (6.59±0.80) and (6.48±0.36) times, respectively.
  
  
  CONCLUSIONS
  The expression of TCRζ chain was up-regulated, when TCR gene-modified T cells were activated by the stimulation of specific antigens.
authors.affiliation: Henan Cancer Hospital, Zhengzhou 450008, China. jnyinqingsong@163.com
authors.forename: Qing-Song
authors.initials: QS
authors.lastname: Yin
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Li-Jian
authors.initials: LJ
authors.lastname: Yang
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Zha
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Yang-Qiu
authors.initials: YQ
authors.lastname: Li
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D019676:Genes, T-Cell Receptor delta; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D044366:Transition Temperature
chemical_list: D000951:Antigens, Neoplasm; D011948:Receptors, Antigen, T-Cell