title: Growth factor-defined culture medium for human mesenchymal stem cells.
abstract: Human bone marrow-derived mesenchymal stem cells (hMSCs) are potential cellular sources of therapeutic stem cells as they have the ability to proliferate and differentiate into a wide array of mesenchymal cell types such as osteoblasts, chondroblasts and adipocytes. hMSCs have been used clinically to treat patients with graft vs. host disease, osteogenesis imperfect, or alveolar cleft, suggesting that transplantation of hMSCs is comparatively safe as a stem cell-based therapy. However, conventional culture medium for hMSCs contains fetal bovine serum (FBS). In the present study, we developed a growth factor-defined, serum-free medium for culturing hMSCs. Under these conditions, TGF-beta1 promoted proliferation of hMSCs. The expanded hMSC population expressed the human pluripotency markers SSEA-3, -4, NANOG, OCT3/4 and SOX2. Furthermore, double positive cells for SSEA-3 and a mesenchymal cell marker, CD105, were detected in the population. The potential to differentiate into osteoblasts and adipocytes was confirmed. This work provides a useful tool to understand the basic biological properties of hMSCs in culture.
authors.affiliation: JCRB Cell Bank, Laboratory of Cell Cultures, Department of Disease Bioresources, National Institute of Biomedical Innovation, Osaka, Japan.
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Mimura
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kimura
authors.forename: Mitsuhi
authors.initials: M
authors.lastname: Hirata
authors.forename: Daiki
authors.initials: D
authors.lastname: Tateyama
authors.forename: Midori
authors.initials: M
authors.lastname: Hayashida
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Arihiro
authors.initials: A
authors.lastname: Kohara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nikawa
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Okamoto
authors.forename: Miho K
authors.initials: MK
authors.lastname: Furue
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D000071063:Endoglin; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism*; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis; D055746:Stage-Specific Embryonic Antigens / Q000096:biosynthesis; D046509:Tissue Culture Techniques; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D015295:Antigens, Tumor-Associated, Carbohydrate; D016895:Culture Media, Serum-Free; C064206:ENG protein, human; D000071063:Endoglin; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D011956:Receptors, Cell Surface; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D055746:Stage-Specific Embryonic Antigens; D016212:Transforming Growth Factor beta; C052924:stage-specific embryonic antigen-3; C066584:stage-specific embryonic antigen-4