title: Quantitative determination of chemical constituents from seeds of Nigella sativa L. using HPLC-UV and identification by LC-ESI-TOF.
abstract: An HPLC method was developed for the simultaneous determination of nine compounds of Nigella sativa L. The separation was achieved within 23 min by using C18 column material, a water-acetonitrile mobile phase, both containing 0.1% acetic acid gradient system and a temperature of 35 degrees C. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of nine compounds were in the range of 0.09-10 and 0.3-25 microg/mL, respectively. The wavelength used for quantification with the diode array detector was 205 and 260 nm. LC/MS coupled with electrospray ionization interface method is described for the identification of compounds in N. sativa L. samples. This method involved the use of [M+H]+ and [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.
authors.affiliation: University of Mississippi, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University, MS 38677, USA.
authors.forename: Bharathi
authors.initials: B
authors.lastname: Avula
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Zulfiqar
authors.initials: Z
authors.lastname: Ali
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D016227:Benzoquinones / Q000737:chemistry; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D008872:Microwaves; D008969:Molecular Sequence Data; D031881:Nigella sativa / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D014465:Ultrasonics
chemical_list: D016227:Benzoquinones; D012996:Solutions; C003466:thymoquinone