title: Identification of a sequence in the matricellular protein SPARC that interacts with the scavenger receptor stabilin-1.
abstract: SPARC (osteonectin/BM-40), a secreted matricellular protein that promotes cellular deadhesion and motility in wound healing, carcinogenesis, and inflammation, binds to the scavenger receptor stabilin-1 in alternatively activated macrophages and undergoes endocytosis and clearance from the extracellular space. Both SPARC and stabilin-1 are expressed by endothelial cells during inflammation, but their interaction in this context is unknown. We have identified a binding site on SPARC for stabilin-1 by a solid-state peptide array coupled with a modified enzyme-linked immunosorbent assay. A monoclonal antibody that recognizes the identified binding site was also characterized that could be an inhibitor for the SPARC-stabilin-1 interaction in macrophages or endothelial cells.
authors.affiliation: Department of Vascular Biology, Benaroya Research Institute, Seattle, Washington 98101, USA.
authors.forename: Gail
authors.initials: G
authors.lastname: Workman
authors.forename: E Helene
authors.initials: EH
authors.lastname: Sage
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D015676:Osteonectin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040081:Protein Array Analysis; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016201:Receptors, Lymphocyte Homing / Q000235:genetics / Q000378:metabolism*; D051116:Receptors, Scavenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D018411:Spodoptera
chemical_list: D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D000939:Epitopes; D015676:Osteonectin; D016201:Receptors, Lymphocyte Homing; D051116:Receptors, Scavenger; D011994:Recombinant Proteins; C449926:STAB1 protein, human