title: Characterization of bioparticles using a miniature cylindrical ion trap mass spectrometer operated at rough vacuum.
abstract: A miniature cylindrical ion trap mass spectrometer (CIT-MS) equipped with an inexpensive mechanical pump was constructed, and used to measure the masses of cells and microparticles generated by laser induced acoustic desorption ionization. Compared with a previous lab scale quadrupole ion trap mass spectrometer (QIT-MS), the novel miniature CIT-MS had smaller volume (the radius r(0)=5 mm), simpler ion trap fabrication and overall instrument construction, required a lower trapping voltage, and reduced the weight, power and cost of the instrument. The CIT-MS was calibrated using standard polystyrene beads of 2.982 µm diameter. The CIT-MS was used to measure the total dry weight of human red blood cells (RBCs) from a healthy female adult (2.12×10(13) Da) and a patient with anemia (1.35×10(13) Da). The coefficient of variance (CV) for the healthy individual was 21% and that for the patient was 30.4%. The CIT-MS was also applied to guinea pig RBCs and the total dry weight was determined as 1.34×10(13) Da with a CV of 37.9%. These measurements are consistent with previous QIT-MS measurements. The new miniaturized instrument has potential for applications in field-portable, biological and aerosol analysis.
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Caiqiao
authors.initials: C
authors.lastname: Xiong
authors.forename: Gaoping
authors.initials: G
authors.lastname: Xu
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Peng
authors.forename: Zongxiu
authors.initials: Z
authors.lastname: Nie
mesh_terms: D000328:Adult; D000336:Aerosols / Q000032:analysis; D000740:Anemia / Q000378:metabolism; D000818:Animals; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization; D011137:Polystyrenes / Q000737:chemistry*; D014618:Vacuum
chemical_list: D000336:Aerosols; D011137:Polystyrenes