title: Ultrasensitive fluorescence polarization DNA detection by target assisted exonuclease III-catalyzed signal amplification.
abstract: Single stranded DNA sequences can be detected by target assisted exonuclease III-catalyzed signal amplification fluorescence polarization (TAECA-FP). The method offers an impressive detection limit of 83 aM within one hour for DNA detection and exhibits high discrimination ability even against a single base mismatch.
authors.affiliation: Lab of Biosystems and Microanalysis, East China University of Science & Technology, Meilong RD 130, Shanghai, 200237, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yi-Meng
authors.initials: YM
authors.lastname: Guan
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005454:Fluorescence Polarization; D057230:Limit of Detection
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III