title: Radiographic findings of Wistar rats fed with T-2 toxin and Kashin-Beck disease-affected diet.
abstract: OBJECTIVE
  To characterize the features of radiographic abnormalities of the tibial bone in Wistar rats which have been fed T-2 toxin and Kashin-Beck disease (KBD) epidemic district food.
  
  
  METHODS
  Ninety Wistar rats were divided into five groups. Group A was fed with a normal diet as control; group B was fed with a normal diet and T-2 toxin; group C was fed with a low-nutrition diet and T-2 toxin; group D was fed with a low-nutrition diet; and group E was fed with a KBD-affected diet. At 4, 8 and 12 weeks, six rats from each group were radiographed. After radiographic examination, samples of left knees were harvested and stained with hematoxylin and eosin.
  
  
  RESULTS
  At 8 and 12 weeks, there were radiological changes in the epiphyseal plate. Abnormal radiological signs of blurring, thinning and irregularity were seen in groups C and E rats, and the length of tibial bones showed significant difference in the KBD-fed rats and low-nutrition diet combined T-2 toxin rats, compared to the control group rats (P < 0.05). The epiphyseal plates showed more obvious necrosis of chondrocytes in groups C and E.
  
  
  CONCLUSIONS
  A rat model of KBD can be established by a KBD-affected diet; proximal epiphyseal plate and metaphyseal bone of the tibia abnormalities on radiographs and histopathology were present in KBD model rats. A low nutrition diet may be involved the aetiology of KBD, and determination of this should be studied in the future.
authors.affiliation: Orthopedics Department, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Denglu
authors.initials: D
authors.lastname: Yan
authors.forename: Pengde
authors.initials: P
authors.lastname: Kang
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
authors.forename: Zongke
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jianyun
authors.initials: J
authors.lastname: Deng
authors.forename: Fuxing
authors.initials: F
authors.lastname: Pei
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D000818:Animals; D002681:China; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D004196:Disease Outbreaks; D005260:Female; D005506:Food Contamination; D006132:Growth Plate / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D057767:Kashin-Beck Disease / Q000000981:diagnostic imaging / Q000209:etiology / Q000473:pathology*; D008297:Male; D009336:Necrosis; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013264:Stifle / Q000000981:diagnostic imaging / Q000473:pathology; D013605:T-2 Toxin / Q000633:toxicity*; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology
chemical_list: D013605:T-2 Toxin