title: Roles of Ca and secretory pathway Ca-ATPase pump type 1 (SPCA1) in intra-Golgi transport.
abstract: Mechanisms for intra-Golgi transport remain a hotly debated topic. Recently, we published data illuminating a new aspect involved in intra-Golgi transport, namely a release of free cytosolic Ca(2+) ([Ca(2+)](cyt)) from the lumen of Golgi cisternae that is fundamental for the secretion and the progression of newly synthesized proteins through the Golgi apparatus (GA). This increase in [Ca(2+)](cyt) during the late stage of synchronous intra-Golgi transport stimulates the fusion of membranes containing cargo proteins and Golgi cisternae, allowing the progression of proteins through the GA. Subsequent restoration of the basal [Ca(2+)](cyt) is also important for the delivery of cargo to the proper final destination. Additionally, the secretory pathway Ca(2+)-ATPase Ca(2+) pump (SPCA1) plays an essential role at this stage. The fine regulation of membrane fusion is also important for the formation and the maintenance of the Golgi ribbon and SPCA1, which regulates [Ca(2+)](cyt) levels, can be considered a controller of trafficking. This evidence contradicts a model of intra-Golgi transport in which permanent membrane continuity allows cargo diffusion and progression.
authors.affiliation: Department of Molecular Cell Biology; institute for Molecular Bioscience; The University of Queensland; Brisbane, QLD Australia.
authors.forename: Massimo
authors.initials: M
authors.lastname: Micaroni
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Mironov