title: Enhancement of 2,3-butanediol production by Klebsiella oxytoca PTCC 1402.
abstract: Optimal operating parameters of 2,3-Butanediol production using Klebsiella oxytoca under submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respectively. Furthermore, optimum values for temperature, inoculum size, pH, and the shaking speed were determined as 37°C, 8 (g/L), 6.1, and 150 rpm, respectively. The optimal combinations of factors obtained from the proposed DOE methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-Butanediol yield of 44%.
authors.affiliation: Department of Microbiology, Faculty of Sciences, Islamic Azad University, Rasht Branch, P.O. Box 41335-3516, Rasht, Iran.
authors.forename: Maesomeh
authors.initials: M
authors.lastname: Anvari
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Safari Motlagh
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000138:chemical synthesis*; D005285:Fermentation / Q000502:physiology; D041121:Klebsiella oxytoca / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol