title: A rapid and simple method to obtain canine peripheral blood-derived macrophages.
abstract: Macrophages play an important role in a variety of situations, including pathogen elimination, inflammation, and tissue repair. However, these cells are not fully studied in dogs, in part, due to the difficulty of efficiently isolating and culturing them in vitro. In this study, we cultured canine peripheral blood mononuclear cells (PBMCs) with 10 ng/ml of phorbol 12-myristate-13-acetate (PMA) for 5 days to obtain macrophages. A high number of round-adherent cells were obtained without the addition of any cytokine. These cells showed active phagocytic activity and a cell surface antigen profile different from dendritic cells. Our method facilitates a high yield of macrophages in a short cultivation period compared with previous studies. This method might be a powerful tool to study macrophage functions in dogs.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. ayukogk@mail.ecc.u-tokyo.ac.jp
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Mayumi
authors.initials: M
authors.lastname: Nakajima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mochizuki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanemoto
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood* / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D006651:Histocytochemistry; D016130:Immunophenotyping / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology