title: Sumoylation of vimentin354 is associated with PIAS3 inhibition of glioma cell migration.
abstract: The invasive phenotype of glioblastoma multiforme (GBM) is a hallmark of malignant process, yet the molecular mechanisms that dictate this locally invasive behavior remain poorly understood. Over-expression of PIAS3 effectively changes cell shape and inhibits GBM cell migration. We focused on the molecular target(s) of PIAS3 stimulated sumoylation, which play an important role in the inhibition of GBM cell motility. Here we report, through the immunoprecipitation with SUMO1 antibody, followed by proteomic analysis, the identification of vimentin (vimentin354), a nuclear component in GBM cells, as the main target of sumoylation promoted by PIAS3.
authors.affiliation: School of Life Science, Lanzhou University, Lanzhou, P. R. China.|Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, USA.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: Tartis, Inc., Buffalo, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, USA.
authors.forename: Sipra
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Molecular Genetics, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Barnes
authors.affiliation: Molecular Genetics, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, USA.
authors.forename: Venkateswara
authors.initials: V
authors.lastname: Sajja
authors.affiliation: Department of Chemistry, Cleveland State University, Cleveland, USA.
authors.forename: Yiding
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Cleveland State University, Cleveland, USA.
authors.forename: Baochuan
authors.initials: B
authors.lastname: Guo
authors.affiliation: School of Life Science, Lanzhou University, Lanzhou, P. R. China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Du
authors.affiliation: Invenio Therapeutics, Inc., Cleveland, USA.
authors.forename: Mukesh K
authors.initials: MK
authors.lastname: Agarwal
authors.affiliation: Department of Pathology, Case Western Reserve University, Cleveland, USA.
authors.forename: David N
authors.initials: DN
authors.lastname: Wald
authors.affiliation: School of Life Science, Lanzhou University, Lanzhou, P. R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Life Science, Lanzhou University, Lanzhou, P. R. China.|Molecular Genetics, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, USA.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D047468:Immunoprecipitation; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D040901:Proteomics; D025842:SUMO-1 Protein / Q000378:metabolism; D058207:Sumoylation / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; C484745:PIAS3 protein, human; D050828:Protein Inhibitors of Activated STAT; D025842:SUMO-1 Protein; D014746:Vimentin