title: Generation of two modified mouse alleles of the Hic1 tumor suppressor gene.
abstract: HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine "reporter" mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence.
authors.affiliation: Department of Cell and Developmental Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic.
authors.forename: Vendula
authors.initials: V
authors.lastname: Pospichalova
authors.forename: Jolana
authors.initials: J
authors.lastname: Tureckova
authors.forename: Bohumil
authors.initials: B
authors.lastname: Fafilek
authors.forename: Martina
authors.initials: M
authors.lastname: Vojtechova
authors.forename: Michaela
authors.initials: M
authors.lastname: Krausova
authors.forename: Jan
authors.initials: J
authors.lastname: Lukas
authors.forename: Eva
authors.initials: E
authors.lastname: Sloncova
authors.forename: Sylvia
authors.initials: S
authors.lastname: Takacova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Divoky
authors.forename: Dominique
authors.initials: D
authors.lastname: Leprince
authors.forename: Jiri
authors.initials: J
authors.lastname: Plachy
authors.forename: Vladimir
authors.initials: V
authors.lastname: Korinek
mesh_terms: D000483:Alleles; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D017930:Genes, Reporter; D016147:Genes, Tumor Suppressor; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C096870:Hic1 protein, mouse; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C045073:Cre recombinase; D019426:Integrases