title: A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
abstract: OBJECTIVE
  To establish a cornea transplant model in a pigmented rat strain and to define the immunologic reaction toward corneal allografts, by studying the cellular and humoral immune response after keratoplasty.
  
  
  METHODS
  Full thickness penetrating keratoplasty was performed on Brown Norway (RT1n) recipients using fully major histocompatibility complex (MHC)-mismatched Piebald-Viral-Glaxo (PVG; RT1c) donors. Using multicolor flow cytometry (FACS) we quantified and compared the cellular composition of draining versus non-draining lymph nodes (LN). Furthermore, we developed an isolation method to release viable graft infiltrating lymphocytes (GIL) and subjected them to phenotypic analysis and screened serum from transplanted animals for allo-antibodies.
  
  
  RESULTS
  Assessing ipsi-lateral submandibular LN we find ample evidence for post surgical inflammation such as elevated absolute numbers of cluster of differentiation (CD)4+, CD8+, B-cells, and differential expression of CD134. However, we could not unequivocally identify an allo-antigen-specific immune response. FACS analysis of lymphocytes isolated from collagenase digested rejected corneas revealed the following six distinct subpopulations: MHC-2+ cells, CD4+ T-cells, CD8+ T-cells, CD161(dull) large granular lymphocytes, CD3+ CD8+ CD161(dull) natural killer (NK)-T-cells and CD161(high) CD3⁻ NK cells. At post-operation day (POD)-07 only CD161(dull) MHC-2(neg) large granular lymphocytes (LGLs) were detected in syngeneic and allo-grafts. In concordance with an increase in B-cell numbers we often detected copious amounts of allo-antibodies in serum of rejecting animals, in particular immunoglobulin (Ig) M (IgM), immunoglobulin (Ig) G1 (IgG1), and IgG2a.
  
  
  CONCLUSIONS
  Our results demonstrate that despite its immune privileged status and low-responder characteristics of the strain combination, allogeneic corneal grafts mount a full fledged T helper1 (Th1) and Th2 response. The presence of NK-T-cells and NK-cells in rejecting corneas shows the synergy between innate and adaptive immunity during allograft destruction.
authors.affiliation: College of Medicine, Nursing and Health Sciences, Regenerative Medicine Institute, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Galway, Ireland.
authors.forename: Martin
authors.initials: M
authors.lastname: Maenz
authors.forename: Mourice
authors.initials: M
authors.lastname: Morcos
authors.forename: Thomas
authors.initials: T
authors.lastname: Ritter
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000096:biosynthesis; D015704:CD4 Antigens / Q000096:biosynthesis; D016039:Corneal Transplantation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006084:Graft Rejection; D007107:Immune System; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007518:Isoantibodies / Q000737:chemistry; D008198:Lymph Nodes / Q000166:cytology* / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism; D008285:Major Histocompatibility Complex; D010641:Phenotype; D051381:Rats
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies