title: Effects of oral acrylamide intake on blood viscosity parameters in rats.
abstract: Acrylamide which is formed via reaction of reducing sugars with amino acids during food processing at high temperatures is not only neurotoxic and carcinogenic, but it also damages erythrocyte membrane and generates micronucleated erythrocytes. In the present study, effects of chronic administration of acrylamide at a dose which does not induce neurotoxicity were evaluated on blood viscosity parameters (hematocrit, erythrocyte deformability, erythrocyte aggregation and plasma viscosity). Twenty adult male Sprague-Dawley rats were divided into control and acrylamide groups. The acrylamide group received 10 mg/kg/day acrylamide, whereas the control group received saline (vehicle), both in 10 ml/kg/day volume via gastric gavage. Erythrocyte aggregation and deformability were measured with LORCA and plasma viscosity with cone-plate viscometer. Erythrocyte deformability was measured before, and at the end of the 3rd and the 5th weeks of acrylamide administration. Hematocrit, erythrocyte aggregation and plasma viscosity were measured only at the end of the 5th week. Acrylamide caused a significant decrease in the deformability index of erythrocytes (at the end of the 3rd week, control: 0.606 ± 0.003, acrylamide: 0.595 ± 0.003, p < 0.05) (at the end of the 5th week, control: 0.606 ± 0.002, acrylamide: 0.588 ± 0.002, p < 0.01). Aggregation tendency and plasma viscosity were slightly higher in the acrylamide group, however the difference was not statistically significant. These results imply that acrylamide which does not cause neurotoxicity in rats may alter blood viscosity if chronically taken.
authors.affiliation: Faculty of Medicine, Department of Physiology, Hacettepe University, Ankara, Turkey. okan.arihan@hacettepe.edu.tr
authors.forename: Okan
authors.initials: O
authors.lastname: Arıhan
authors.forename: Nurten B
authors.initials: NB
authors.lastname: Seringeç
authors.forename: Esin İleri
authors.initials: Eİ
authors.lastname: Gürel
authors.forename: Neslihan H
authors.initials: NH
authors.lastname: Dikmenoğlu
mesh_terms: D020106:Acrylamide / Q000494:pharmacology*; D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects*; D002273:Carcinogens / Q000494:pharmacology*; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004907:Erythrocyte Deformability / Q000187:drug effects*; D006400:Hematocrit; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002273:Carcinogens; D020106:Acrylamide