title: Rice bran fermented with saccharomyces boulardii generates novel metabolite profiles with bioactivity.
abstract: Emerging evidence supporting chronic disease fighting properties of rice bran has advanced the development of stabilized rice bran for human use as a functional food and dietary supplement. A global and targeted metabolomic investigation of stabilized rice bran fermented with Saccharomyces boulardii was performed in three rice varieties. Metabolites from S. boulardii-fermented rice bran were detected by gas chromatography-mass spectrometry (GC-MS) and assessed for bioactivity compared to nonfermented rice bran in normal and malignant lymphocytes. Global metabolite profiling revealed significant differences in the metabolome that led to discovery of candidate compounds modulated by S. boulardii fermentation. Fermented rice bran extracts from three rice varieties reduced growth of human B lymphomas compared to each variety's nonfermented control and revealed that fermentation differentially altered bioactive compounds. These data support that integration of global and targeted metabolite analysis can be utilized for assessing health properties of rice bran phytochemicals that are enhanced by yeast fermentation and that differ across rice varieties.
authors.affiliation: Department of Clinical Sciences, Colorado State University , Fort Collins, Colorado 80523, United States. Ryan@colostate.edu
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Ryan
authors.forename: Adam L
authors.initials: AL
authors.lastname: Heuberger
authors.forename: Tiffany L
authors.initials: TL
authors.lastname: Weir
authors.forename: Brittany
authors.initials: B
authors.lastname: Barnett
authors.forename: Corey D
authors.initials: CD
authors.lastname: Broeckling
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Prenni
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D016393:Lymphoma, B-Cell; D055432:Metabolomics; D012275:Oryza / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology; D019936:Probiotics; D012440:Saccharomyces / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*
chemical_list: D010936:Plant Extracts