title: Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet.
abstract: The goal of the present study was to investigate whether subchronic treatment with grape juice concentrate is able to protect liver and peripheral blood cells against cholesterol-induced injury in rats. The effects of the grape juice concentrate treatment on histopathological changes, immunohistochemistry for cyclo-oxygenase-2 (COX-2), and basal and oxidative DNA damage induced by H2O2 using a single-cell gel (comet) assay were evaluated. Male Wistar rats (n 18) were divided into three groups: group 1--negative control; group 2--cholesterol at 1 % (w/w) in their diet, treated for 5 weeks; group 3--cholesterol at 1 % in their chow, treated for 5 weeks, and grape juice concentrate at 222 mg/d in their drinking-water in the final week only. The results indicated that the treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in group 3 compared with group 2. However, grape juice concentrate was able to decrease oxidative DNA damage induced by H2O2 in peripheral blood cells, as depicted by the tail moment results. COX-2 expression in the liver did not show statistically significant differences (P>0·05) between groups. Taken together, the present results suggest that the administration of subchronic grape juice concentrate prevents oxidative DNA damage in peripheral blood cells.
authors.affiliation: Departamento de Biociências, Universidade Federal de São Paulo (UNIFESP), Avenida Ana Costa, 95 Vila Mathias, Santos, SP, Brazil.
authors.forename: Odair
authors.initials: O
authors.lastname: Aguiar
authors.forename: Andréa Pittelli Boiago
authors.initials: AP
authors.lastname: Gollücke
authors.forename: Bárbara Bueno
authors.initials: BB
authors.lastname: de Moraes
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pasquini
authors.forename: Rodrigo Ramos
authors.initials: RR
authors.lastname: Catharino
authors.forename: Maria Francesca
authors.initials: MF
authors.lastname: Riccio
authors.forename: Silvia Saiuli Miki
authors.initials: SS
authors.lastname: Ihara
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001773:Blood Cells / Q000187:drug effects*; D002791:Cholesterol, Dietary / Q000009:adverse effects; D020552:Comet Assay; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004249:DNA Damage; D004036:Diet, Atherogenic; D005638:Fruit; D006861:Hydrogen Peroxide; D006937:Hypercholesterolemia / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis
chemical_list: D000975:Antioxidants; D002791:Cholesterol, Dietary; D028321:Plant Preparations; D006861:Hydrogen Peroxide; D051546:Cyclooxygenase 2