title: Analysis of saikosaponins in Bupleuri Radix and Caihu-shugan-san using reversed-phase HPLC with pulsed amperometric detection.
abstract: A simple and sensitive reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD) method was developed to determine the saikosaponin content in Bupleuri Radix or Caihu-shugan-san. Four saikosaponins in Bupleuri Radix and Caihu-shugan-san were extracted with a 6:4 solution of 10 mM sodium phosphate buffer (pH 8)/100% ethanol. Pulsed amperometric detection of carbohydrates in four major saikosaponins was highly sensitive when used with a water-acetonitrile gradient on an alkaline RP column with a post-column delivery system. The limits of detection (S/N=3) and of quantification (S/N=10) of saikosaponins were 0.01-0.02 and 0.03-0.05 μg/mL, respectively. The intra- and inter-day precision (RSDs) were each <9.7% and the average recoveries were 95.0-97.6% in Bupleuri Radix. This method can be used to analyze saikosaponins in Bupleuri Radix and Caihu-shugan-san.
authors.affiliation: Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea.
authors.forename: Ha-Jeong
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Sim
authors.forename: Sa-im
authors.initials: SI
authors.lastname: Lee
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Yong-Duk
authors.initials: YD
authors.lastname: Park
authors.forename: Seon-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D012503:Saponins / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D