title: Flow distribution during infusion of UW and HTK solution in anaesthetised rats.
abstract: OBJECTIVE
  Organ transplantation necessitates the use of preservation solutions to maintain the integrity of the organs during retrieval. The aim of this study was to investigate the flow distribution in abdominal organs in rats during acute infusion of preservation solution.
  
  
  METHODS
  Microspheres were used to estimate the distribution of flow in the pancreas, duodenum, ileum, colon, liver, kidneys and lungs in untreated Wistar-Furth rats and in animals with an opened abdominal cavity and catheterised aorta. Some animals were infused by free flow of 5 ml of UW, HTK or Ringer solution containing microspheres at a pressure of 100 cm H(2)O through an intra-aortic catheter.
  
  
  RESULTS
  Opening of the abdominal cavity did not affect any of the organ blood flow values. However, the fraction of total pancreatic blood flow diverted through the islets increased. During infusion of microsphere-containing UW, HTK or Ringer solution, splanchnic and renal organ flow values, represented by microsphere contents, were similar. The fraction of microspheres found in the islets was lower in UW-infused rats. The number of microspheres present in the lungs or liver was very low, suggesting that shunting was negligible.
  
  
  CONCLUSIONS
  Infusion of HTK and UW solution into anaesthetised rats results in a flow distribution which is similar to that in normal animals in most abdominal organs, but there is a reduction in islet blood perfusion by UW but not HTK solution.
authors.affiliation: Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Box 571, 751 23, Uppsala, Sweden. Leif.Jansson@mcb.uu.se
authors.forename: Leif
authors.initials: L
authors.lastname: Jansson
authors.forename: Per-Ola
authors.initials: PO
authors.lastname: Carlsson
authors.forename: Birgitta
authors.initials: B
authors.lastname: Bodin
authors.forename: Orjan
authors.initials: O
authors.lastname: Källskog
mesh_terms: D000241:Adenosine; D000311:Adrenal Glands / Q000098:blood supply; D000493:Allopurinol; D000768:Anesthesia, General; D000818:Animals; D001012:Aorta, Abdominal; D001783:Blood Flow Velocity / Q000502:physiology; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D002408:Catheters, Indwelling; D003106:Colon / Q000098:blood supply; D004386:Duodenum / Q000098:blood supply; D005947:Glucose; D005978:Glutathione; D006400:Hematocrit; D007328:Insulin; D007515:Islets of Langerhans / Q000098:blood supply; D007668:Kidney / Q000098:blood supply; D008099:Liver / Q000098:blood supply; D008168:Lung / Q000098:blood supply; D008297:Male; D008353:Mannitol; D008863:Microspheres; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D016377:Organ Transplantation; D010179:Pancreas / Q000098:blood supply; D011189:Potassium Chloride; D011343:Procaine; D011887:Raffinose; D051381:Rats; D011920:Rats, Inbred WF; D012039:Regional Blood Flow / Q000502:physiology; D013152:Splanchnic Circulation / Q000502:physiology*; D014018:Tissue Distribution; D014661:Vasoconstriction / Q000502:physiology
chemical_list: D001786:Blood Glucose; C039984:Bretschneider cardioplegic solution; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose