title: Infection with human coronavirus NL63 enhances streptococcal adherence to epithelial cells.
abstract: Understanding the mechanisms of augmented bacterial pathogenicity in post-viral infections is the first step in the development of an effective therapy. This study assessed the effect of human coronavirus NL63 (HCoV-NL63) on the adherence of bacterial pathogens associated with respiratory tract illnesses. It was shown that HCoV-NL63 infection resulted in an increased adherence of Streptococcus pneumoniae to virus-infected cell lines and fully differentiated primary human airway epithelium cultures. The enhanced binding of bacteria correlated with an increased expression level of the platelet-activating factor receptor (PAF-R), but detailed evaluation of the bacterium-PAF-R interaction revealed a limited relevance of this process.
authors.affiliation: Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Golda
authors.affiliation: Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Malek
authors.affiliation: Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Dudek
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41-800 Zabrze, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Zeglen
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41-800 Zabrze, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Wojarski
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41-800 Zabrze, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Ochman
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41-800 Zabrze, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kucewicz
authors.affiliation: Department of Cardiac Surgery and Transplantology, Silesian Center for Heart Diseases, Szpitalna 2, 41-800 Zabrze, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Zembala
authors.affiliation: University of Louisville School of Dentistry, Department of Oral Health and Rehabilitation, 501 South Preston St, Louisville, KY 40202, USA.|Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Potempa
authors.affiliation: Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pyrc
mesh_terms: D001422:Bacterial Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D058957:Coronavirus NL63, Human / Q000502:physiology*; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology* / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D012141:Respiratory Tract Infections / Q000235:genetics / Q000378:metabolism / Q000382:microbiology* / Q000821:virology*; D013291:Streptococcus / Q000502:physiology*
chemical_list: D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor