title: [Protective effect of limb ischemic preconditioning against liver ischemia/reperfusion injury in the rat].
abstract: OBJECTIVE
  To study the protective effect of limb ischemic preconditioning against liver ischemia/reperfusion injury in the rat.
  
  
  METHODS
  Rats were randomly divided into four groups (n = 8): (1) Sham group (S group), rats without ischemic preconditioning (IPC), (2) Ischemia/reperfusion (I/R) without IP (I/R group); (3) Rats with 5 min IPC (IPC group); (4) Rats with lower limbs IPC and repeated three times (remote ischemic preconditioning, RPC group); The rats were subjected to 60-min sustained liver ischemia followed by 180-min reperfusion except S group. All ischemia rats were only subjected to 70% liver ischemia. Finally, blood and liver samples were obtained to determine the activity of ALT and AST, liver wet/dry weight (W/D), PMN counts and pathology.
  
  
  RESULTS
  All IPC group and RPC group had obviously lower levels of ALT, AST, W/D, PMN counts than that of the I/R group (P < 0.01).
  
  
  CONCLUSIONS
  The limb ischemic preconditioning has a protective effects against liver ischemia/reperfusion injury in the rat, possibly are due to suppression of liver inflammatory reaction, improvement of liver microcirculation.
authors.affiliation: Department of General Surgery, Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, China.
authors.forename: Hou-Wen
authors.initials: HW
authors.lastname: Jiang
authors.forename: Chuang
authors.initials: C
authors.lastname: Chen
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Hao
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase