title: Identification of Bcl-2/IgH fusion sequences using real-time PCR and chip-based microcapillary electrophoresis.
abstract: BACKGROUND
  The determination of polymerase chain reaction (PCR) amplification product sizes of the Bcl-2/IgH fusion gene from follicular lymphoma (FL) provides evidence of clonal identity.
  
  
  METHODS
  The present study describes detection of Bcl-2/IgH fusion gene clonality utilizing a small, simple microcapillary electrophoretic chip combined with a real-time PCR method.
  
  
  RESULTS
  The microcapillary electrophoretic chip system effectively detects size differences among the Bcl-2/IgH fusion gene amplification products of FL from patient samples; something that is not possible using traditional gel electrophoresis. We also describe the potential of this system to utilize formalin-fixed, paraffin-embedded tissue samples sectioned on charged slides.
  
  
  CONCLUSIONS
  The simple detection of Bcl-2/IgH fusion gene clonality using a microcapillary electrophoretic chip provides reliable information for monitoring minimal residual disease of FL, and can be an effective tool for use in clinical laboratories.
authors.affiliation: Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Tokyo, Japan. tabe@juntendo.ac.jp
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tabe
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kawase
authors.forename: Kazunori
authors.initials: K
authors.lastname: Miyake
authors.forename: Naotake
authors.initials: N
authors.lastname: Satoh
authors.forename: Nanae
authors.initials: N
authors.lastname: Aritaka
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Isobe
authors.forename: Kazuo
authors.initials: K
authors.lastname: Oshimi
authors.forename: Norio
authors.initials: N
authors.lastname: Komatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
mesh_terms: D002460:Cell Line; D046209:Electrophoresis, Microchip / Q000379:methods*; D005260:Female; D005557:Formaldehyde / Q000378:metabolism; D050939:Gene Fusion / Q000235:genetics*; D050438:Genes, Immunoglobulin Heavy Chain / Q000235:genetics*; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D005557:Formaldehyde