title: Human parthenogenetic stem cells produce enriched populations of definitive endoderm cells after trichostatin A pretreatment.
abstract: Human parthenogenetic stem cells (hpSC) hold great promise as a source of pluripotent stem cells for cell-based transplantation therapy due to their ethical method of derivation as well as the enhanced capacity for immunomatching with significant segments of the human population. We report here the directed differentiation of hpSC to produce enriched populations of definitive endoderm. Moreover, we find that treatment of undifferentiated hpSC by trichostatin A (TSA) before applying the directed differentiation protocol significantly increases the proportion of definitive endoderm cells in the final population. TSA-pretreated as well as non-TSA-treated hpSC undergoing differentiation toward definitive endoderm demonstrate a similar temporal sequence of gene expression to that which occurs in the course of definitive endoderm differentiation during vertebrate gastrulation and for differentiation of hESCs to definitive endoderm. Creation of the definitive endoderm lineages from hpSC represents the critical first step toward the development of hpSC-based cellular therapies for diseases of the liver or pancreas.
authors.affiliation: International Stem Cell Corporation, Oceanside, CA 92056, USA. nturovets@intlstemcell.com
authors.forename: Nikolay
authors.initials: N
authors.lastname: Turovets
authors.forename: Kevin A
authors.initials: KA
authors.lastname: D'Amour
authors.forename: Vladimir
authors.initials: V
authors.lastname: Agapov
authors.forename: Irina
authors.initials: I
authors.lastname: Turovets
authors.forename: Olga
authors.initials: O
authors.lastname: Kochetkova
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Janus
authors.forename: Andrey
authors.initials: A
authors.lastname: Semechkin
authors.forename: Mark A
authors.initials: MA
authors.lastname: Moorman
authors.forename: Larissa
authors.initials: L
authors.lastname: Agapova
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D019070:Cell Lineage; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D010312:Parthenogenesis / Q000235:genetics*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D006877:Hydroxamic Acids; C012589:trichostatin A