title: Interleukin-10 gene promoter polymorphisms and their protein production in pleural fluid in patients with tuberculosis.
abstract: Associations of interleukin-10 (IL-10) gene promoter polymorphisms and pleural tuberculosis risk remain unclear. The objective of this study was to determine IL-10 gene promoter polymorphisms at -1082, -819 and -592 sites and their protein production in pleural fluid (PF) in patients with and without pleural tuberculosis. IL-10 gene promoter polymorphisms at the -1082, -819 and -592 sites were genotyped using a SNaPshot assay. Protein levels of IL-10 in PF were measured using an enzyme-linked immunosorbent assay. There were no significant differences in the genotype and allele frequencies of IL-10 gene promoter polymorphisms at position -1082 between the pleural tuberculosis and the control groups. However, the frequency of -819 T or -592 A alleles was significantly more common in patients with pleural tuberculosis than controls. The protein levels of IL-10 in PF were statistically higher in the pleural tuberculosis group than in the control group. Moreover, the polymorphisms at the -1082, -819 and -592 sites were associated with protein levels of IL-10 in PF in the pleural tuberculosis group, while in the control group, only the polymorphism at position -1082 correlated with the protein levels. These findings support the association between IL-10 promoter polymorphisms at -819 and -592 sites and their protein production with pleural tuberculosis risk.
authors.affiliation: Shanghai Key Laboratory of Mycobacterium Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Yue
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Wang
authors.forename: Heping
authors.initials: H
authors.lastname: Xiao
mesh_terms: D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D010996:Pleural Effusion / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014396:Tuberculosis, Pleural / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D016753:Interleukin-10