title: Procedures to evaluate the importance of dietary polyamines.
abstract: Polyamines not only play vital physiological functions including modulating transcription and translation of genetic material, cell proliferation and growth, ion channel regulation and cell signaling, but have also been cited in the pathogenesis of diseases. Many plant and animal sources used as food contain high amounts of polyamines. Knowledge of the content of polyamines in food as a source of these growth factors is therefore critical. A 2-step perchloric acid precipitation method to obtain acid soluble extracts from food that are subsequently taken through a dansylation process to produce dansyl polyamine derivatives for HPLC measurement is described. Examples are provided to illustrate mathematical correction factors.
authors.affiliation: Division of Applied Medicine, University of Aberdeen, Aberdeen, Scotland, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Acheampong
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Macleod
authors.forename: Heather M
authors.initials: HM
authors.lastname: Wallace
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D045424:Complex Mixtures; D003619:Dansyl Compounds / Q000378:metabolism; D004032:Diet; D005504:Food Analysis / Q000379:methods*; D010472:Perchlorates / Q000737:chemistry; D011073:Polyamines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011700:Putrescine / Q000032:analysis; D012015:Reference Standards; D012995:Solubility; D012996:Solutions; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014050:Toluene / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D045424:Complex Mixtures; D003619:Dansyl Compounds; D010472:Perchlorates; D011073:Polyamines; D012996:Solutions; D013096:Spermine; D014050:Toluene; D013095:Spermidine; D011700:Putrescine; C032159:acetonitrile