title: Cardiotoxicity testing using pluripotent stem cell-derived human cardiomyocytes and state-of-the-art bioanalytics: a review.
abstract: In this article, recent progress in cardiotoxicity testing based on the use of immortalized cell lines or human embryonic stem cell (hESC) derived cardiomyocytes in combination with state-of-the-art bioanalytical methods and sensors is reviewed. The focus is on hESC-derived cells and their refinement into competent testing cells, but the access and utility of other relevant cell types are also discussed. Recent developments in sensor techniques and bioanalytical approaches for measuring critical cardiotoxicity parameters are highlighted, together with aspects of data evaluation and validation. Finally, recommendations for further research are given.
authors.affiliation: Biotechnology/IFM, Linköping University, Linköping, Sweden. cfm@ifm.liu.se
authors.forename: Carl-Fredrik
authors.initials: CF
authors.lastname: Mandenius
authors.forename: Daniella
authors.initials: D
authors.lastname: Steel
authors.forename: Fozia
authors.initials: F
authors.lastname: Noor
authors.forename: Thomas
authors.initials: T
authors.lastname: Meyer
authors.forename: Elmar
authors.initials: E
authors.lastname: Heinzle
authors.forename: Julia
authors.initials: J
authors.lastname: Asp
authors.forename: Sarina
authors.initials: S
authors.lastname: Arain
authors.forename: Udo
authors.initials: U
authors.lastname: Kraushaar
authors.forename: Susanne
authors.initials: S
authors.lastname: Bremer
authors.forename: Reiner
authors.initials: R
authors.lastname: Class
authors.forename: Peter
authors.initials: P
authors.lastname: Sartipy
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D004353:Drug Evaluation, Preclinical; D064420:Drug-Related Side Effects and Adverse Reactions / Q000145:classification; D006331:Heart Diseases / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010101:Oxygen Consumption; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects*; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D015262:Xenobiotics