title: Immunotypes of a quaternary site of HIV-1 vulnerability and their recognition by antibodies.
abstract: HIV-1 is neutralized by a class of antibodies that preferentially recognize a site formed on the assembled viral spike. Such quaternary structure-specific antibodies have diverse neutralization breadths, with antibodies PG16 and PG9 able to neutralize 70 to 80% of circulating HIV-1 isolates while antibody 2909 is specific for strain SF162. We show that alteration between a rare lysine and a common N-linked glycan at position 160 of HIV-1 gp120 is primarily responsible for toggling between 2909 and PG16/PG9 neutralization sensitivity. Quaternary structure-specific antibodies appear to target antigenic variants of the same epitope, with neutralization breadth determined by the prevalence of recognized variants among circulating isolates.
authors.affiliation: Vaccine Research Center, NIAID, NIH, 40 Convent Drive, Bethesda, MD 20892, USA.
authors.forename: Xueling
authors.initials: X
authors.lastname: Wu
authors.forename: Anita
authors.initials: A
authors.lastname: Changela
authors.forename: Sijy
authors.initials: S
authors.lastname: O'Dell
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Schmidt
authors.forename: Marie
authors.initials: M
authors.lastname: Pancera
authors.forename: Yongping
authors.initials: Y
authors.lastname: Yang
authors.forename: Baoshan
authors.initials: B
authors.lastname: Zhang
authors.forename: Miroslaw K
authors.initials: MK
authors.lastname: Gorny
authors.forename: Sanjay
authors.initials: S
authors.lastname: Phogat
authors.forename: James E
authors.initials: JE
authors.lastname: Robinson
authors.forename: Leonidas
authors.initials: L
authors.lastname: Stamatatos
authors.forename: Susan
authors.initials: S
authors.lastname: Zolla-Pazner
authors.forename: Peter D
authors.initials: PD
authors.lastname: Kwong
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006031:Glycosylation; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D008239:Lysine / Q000378:metabolism
chemical_list: D057134:Antibodies, Neutralizing; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C514793:gp120 protein, Human immunodeficiency virus 1; D008239:Lysine