title: Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids.
abstract: Phytoene synthase (PSY) catalyses the first step in the production of carotenoids, which has been described as a key regulatory step in the carotenoids biosynthetic pathway. PSY gene from Dunaliella salina was constitutively expressed in Chlamydomonas reinhardtii under the control of the RBCS2 and HSP70A promoters and targeted to the chloroplast by the RBCS2 transit peptide. DsPSY overexpression resulted in a stable increase in the corresponding PSY transcript level and in the content of carotenoids such as violaxanthin, lutein, and β-carotene, reaching between 125 and 260% the levels in control untransformed cells.
authors.affiliation: Departamento de Química y Ciencia de Materiales, Lab Bioquímica y Biología Molecular, Facultad de Ciencias Experimentales, University of Huelva, Avda. Fuerzas Armadas s/n, Huelva 21007, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Couso
authors.forename: M
authors.initials: M
authors.lastname: Vila
authors.forename: H
authors.initials: H
authors.lastname: Rodriguez
authors.forename: M A
authors.initials: MA
authors.lastname: Vargas
authors.forename: R
authors.initials: R
authors.lastname: León
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D002338:Carotenoids / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000201:enzymology*; D005816:Genetic Complementation Test; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002338:Carotenoids; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase