title: Effects of ozone oxidative preconditioning on different hepatic biomarkers of oxidative stress in endotoxic shock in mice.
abstract: In endotoxic shock, variations are known to occur in different biochemical parameters of oxidative stress. Ozone oxidative preconditioning (OOP) is a good candidate to restore the redox balance on different tissue. This investigation examined the effect of OOP on different biomarkers of oxidative stress in hepatic tissue of mice treated with lipopolysaccharide (LPS). LPS doses of 30 mg/kg were administered intraperitoneally (i.p.) and pretreatment with ozone/oxygen mixture (OOM) was applied i.p. at 0.2, 0.4, and 1.2 mg/kg once daily during 5 days before LPS injection. The mice were euthanized under ether atmosphere at different times, 1 and 24 h after LPS injection. Hepatic tissue from all animals was taken for biochemical determinations of oxidative stress parameters such as thiobarbituric acid reactive substances (TBARS) content and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione S-transferase (GST). The results demonstrated that OOP reduces levels of TBARS content and increases the activity of GPx in hepatic tissue. In conclusion, OOP was able to recover the redox balance and in this way to protect the animals against the oxidative damage induced by endotoxemia.
authors.affiliation: Department of Biomedical Research, Laboratory of Biological Assays, Ozone Research Center, Havana, Cuba. zullyt.zamora@cnic.edu.cu
authors.forename: Zullyt Zamora
authors.initials: ZZ
authors.lastname: Rodríguez
authors.forename: Dailén
authors.initials: D
authors.lastname: Guanche
authors.forename: Ricardo González
authors.initials: RG
authors.lastname: Alvarez
authors.forename: Yaima
authors.initials: Y
authors.lastname: Martinez
authors.forename: Yaima
authors.initials: Y
authors.lastname: Alonso
authors.forename: Siegfried
authors.initials: S
authors.lastname: Schulz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010083:Oxidants, Photochemical / Q000494:pharmacology* / Q000627:therapeutic use; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology* / Q000627:therapeutic use; D012772:Shock, Septic / Q000188:drug therapy / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances
chemical_list: D015415:Biomarkers; D004731:Endotoxins; D010083:Oxidants, Photochemical; D017392:Thiobarbituric Acid Reactive Substances; D010126:Ozone