title: Optimized grafting of antimicrobial peptides on stainless steel surface and biofilm resistance tests.
abstract: Antibacterial peptides, magainin I and nisin were covalently bound to stainless steel surfaces. Several procedures of surface functionalisation processes have been investigated and optimized, each step being characterized by polarization modulation reflection absorption infrared spectroscopy (PM-RAIRS) and X-ray photoemission spectroscopy (XPS). Grafting of antibacterial peptides was successfully achieved by a 3 steps functionalisation process on a chitosan polymeric layer. The antibacterial activity of the anchored magainin and nisin was tested against a gram-positive bacteria, Listeria ivanovii, i.e., the possible survival and attachment of this bacteria, was characterized on modified stainless steel surfaces. The results revealed that the adsorbed peptides reduced the adhesion of bacteria on the functionalised stainless steel surface.
authors.affiliation: Laboratoire de Réactivité de Surface, UMR CNRS 7197, Université Pierre et Marie Curie, UPMC Paris 6, 4 place Jussieu, 75252 Paris Cedex 05, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Héquet
authors.forename: Vincent
authors.initials: V
authors.lastname: Humblot
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Berjeaud
authors.forename: Claire-Marie
authors.initials: CM
authors.lastname: Pradier
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D001422:Bacterial Adhesion; D018441:Biofilms / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D015394:Molecular Structure; D009561:Nisin / Q000737:chemistry / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D003432:Cross-Linking Reagents; D010455:Peptides; D013193:Stainless Steel; D009561:Nisin; D048271:Chitosan