title: CpG oligodeoxynucleotide synergizes innate defense regulator peptide for enhancing the systemic and mucosal immune responses to pseudorabies attenuated virus vaccine in piglets in vivo.
abstract: Oligonucleotides containing CpG motifs (CpG ODN) are strong adjuvants for humoral and cellular immune responses in mice, and innate defense-regulator peptides (IDRs) are known to facilitate the uptake of antigens into antigen presenting cells (APCs), but data on synergistic effects of CpG and IDRs in piglets are scarce. In this report, the combination of porcine-specific CpG ODN and HH2 (a kind of IDR which was selected for its better synergy with CpG ODN) was used as immunoadjuvant to enhance the immune responses of the newborn piglets to Pseudorabies attenuated virus (PRV) vaccine. The titers of specific antibodies and serum IgG1/IgG2 subtypes to PRV vaccine, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), IL-12 and IL-4 were examined to identify the immune responses of the newborn piglets. The results showed that piglets immunized intranasally (IN) and subcutaneously (SC) with PRV vaccine and CpG-HH2 complex both presented high titers of PRV-specific antibodies and IgG2 isotype, a Th1-dominated (IFN-γ and IL-12) cytokine profiles, high levels of IgA in saliva, broncheoalveolar lavage (BAL) and intestinal washings. The results suggested that, CpG-HH2 complex augmented systemic (IgG in serum) and mucosal (IgA in saliva, BAL and intestinal washings) immune responses against antigen. CpG-HH2 complex stimulated both T-helper type1 (Th1) (IgG2) and Th2 (IgA) responses when delivered IN, and IN route could induce stronger mucosal immune responses than SC route. All these data indicate that CpG-HH2 complex is a potential effective adjuvant for the PRV vaccine in newborn piglets.
authors.affiliation: College of Life Sciences, South China Agricultural University, Wushan Road, Guangzhou, GuangDong, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Cao
authors.forename: Huazhou
authors.initials: H
authors.lastname: Li
authors.forename: Zhenggu
authors.initials: Z
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Cheng
authors.forename: Zhaihan
authors.initials: Z
authors.lastname: Yang
authors.forename: Chenchao
authors.initials: C
authors.lastname: Xu
authors.forename: Guangjun
authors.initials: G
authors.lastname: Cao
authors.forename: Linghua
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000831:Animals, Newborn; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage*; D016207:Cytokines / Q000097:blood; D004333:Drug Administration Routes; D004357:Drug Synergism; D011558:Herpesvirus 1, Suid / Q000276:immunology* / Q000472:pathogenicity; D056724:Immunity, Humoral / Q000187:drug effects; D018928:Immunity, Mucosal / Q000187:drug effects; D007074:Immunoglobulin G / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000008:administration & dosage; D013552:Swine; D058408:Th1-Th2 Balance; D014613:Vaccines, Attenuated / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D023181:Antimicrobial Cationic Peptides; C408982:CPG-oligonucleotide; D016207:Cytokines; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D022421:Pseudorabies Vaccines; D014613:Vaccines, Attenuated