title: [Effect of curcumine on the nuclear pathway of JNK during hippocampal ischemia/reperfusion injury in SHR].
abstract: OBJECTIVE
  To investigate the diversify of the nuclear pathway of c-Jun NH2-terminal kinases (JNK) during transient brain ischemia/reperfusion injury in hippocampal neuron apoptosis in spontaneously hypertensive rats (SHR) and to test whether the neuroprotection of curcumine on transient brain ischemia/reperfusion injury in SHR is related to the nuclear pathway of JNK.
  
  
  METHODS
  Male Wistar-Kyoto (WKY) rats and SHR were randomly divided into five groups (n = 6): WKY sham group (W-Sham), WKY ischemia/reperfusion group (W-I/ R), SHR sham group (S-Sham), SHR ischemia/reperfusion group (S-I/R) and SHR curcumine (a chinese traditional medicine)100 mg/kg treatment group (S-Cur), which were sacrificed at 2 h, 6 h, 24 h, 3 d and 7 d after reperfusion. Global brain ischemic model was established by 4-VO method. The TdT-mediated dUTP nick end labeling (TUNEL) method was used to detect the neuron apoptosis in hippocampal CA1 region. The immunohistochemical method was applied to investigate the expressions of c-jun and c-fos in hippocampal CA1 region.
  
  
  RESULTS
  The expressions of apoptosis and c-jun and c-fos in CA1 region in S-Sham group, W-I/R group and S-I/R group were more than those in W-Sham group (P < 0.05), were significantly increased in S-I/R group than those in W-I/R group (P < 0.05), and were significantly decreased in S-Cur group than those in S-I/R group (P < 0.05).
  
  
  CONCLUSIONS
  Neuronal apoptosis and the expressions of c-jun and c-fos are more in SHR hippocampal. Global brain ischemia/reperfusion injury induces more expressions of apoptosis in hippocampal neuron in SHR, and the more expressions of c-jun and c-fos may participate in that process. The neuroprotection of curcumine in SHR is related to c-jun and c-fos.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Ke-Ping
authors.initials: KP
authors.lastname: Ye
authors.forename: Chun-Ru
authors.initials: CR
authors.lastname: Chen
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Meng
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Qing-Quan
authors.initials: QQ
authors.lastname: Lian
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D048031:JNK Mitogen-Activated Protein Kinases; D003474:Curcumin