title: In vivo toxicity of nano-alumina on mice neurobehavioral profiles and the potential mechanisms.
abstract: The rapid development and expanding applications of nanotechnology have led to enhanced exposure of human body to nanoparticles. It is, therefore, necessary to address the safety issue via rigorous toxicological evaluation and to understand the underlying interaction mechanism. However, only a few studies to date have evaluated the safety of nano-sized materials and their potential adverse effects on biological systems. In this study, we sought to investigate the potential toxicity of aluminum oxide (alumina) nanoparticles in ICR strained mice, focusing on potential neurobehavioral defects and the possible mechanisms. The results demonstrated that nano-alumina impaired neurobehavioral functions, including lengthened escape latency, shorter time spent in the target quadrant and reductions in the number of platform crossing. In addition, it induced cell necrosis and apoptosis, which were likely mediated by the reduction of MMP and ROS, and the induction of the caspase-3 gene. Our results implicated that mitochondrial impairment plays a key role in neurotoxicity of nano-alumina, sequent oxidative damage and neural cell loss, especially necrosis, may be direct causes for the neurobehavioral defects. Collectively, nano-alumina presents a strong pro-cell death effect on ICR mice in vivo, suggesting that nano-alumina may serve as an inducer for neural toxicology. Findings in the present study indicating that surface chemical characteristics and nanoscale sizes of nano-alumina could co-contribute significantly to neurotoxicity. The impaired neurobehavioral patterns indicate that nano-alumina particles are more toxic to the cerebrum than those of nano-carbon with the same nanoparticle size and micro-alumina with the same surface chemical characteristics.
authors.affiliation: Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan, PRC. zhangql930611@126.com
authors.forename: Q L
authors.initials: QL
authors.lastname: Zhang
authors.forename: M Q
authors.initials: MQ
authors.lastname: Li
authors.forename: J W
authors.initials: JW
authors.lastname: Ji
authors.forename: F P
authors.initials: FP
authors.lastname: Gao
authors.forename: R
authors.initials: R
authors.lastname: Bai
authors.forename: C Y
authors.initials: CY
authors.lastname: Chen
authors.forename: Z W
authors.initials: ZW
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Niu
mesh_terms: D000537:Aluminum Oxide / Q000633:toxicity*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects*; D005434:Flow Cytometry; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D008813:Mice, Inbred ICR; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D000537:Aluminum Oxide