title: Identification of a novel fibronectin-aggrecan complex in the synovial fluid of knees with painful meniscal injury.
abstract: BACKGROUND
  Molecular biomarkers associated with knee pain may be useful as diagnostic modalities, prognostic indicators, and surrogate end points for therapeutic trials. The present study describes a novel complex of fibronectin and aggrecan that is present in the affected knee of patients with pain and meniscal abnormality.
  
  
  METHODS
  The present prospective study included thirty patients with knee pain, mechanical symptoms, and magnetic resonance imaging findings that were positive for a meniscal tear who chose arthroscopic partial meniscectomy after unsuccessful nonoperative management. Synovial fluid was aspirated at the time of surgery and was assayed for the fibronectin-aggrecan complex with use of a heterogeneous enzyme-linked immunosorbent assay (ELISA). The results were compared with knee aspirates from ten asymptomatic volunteers with no pain who underwent magnetic resonance imaging of the knee.
  
  
  RESULTS
  The mean optical density (and standard deviation) of the fibronectin-aggrecan complex was significantly greater in synovial fluid from knees undergoing arthroscopic surgery as compared with fluid from asymptomatic controls (13.29 ± 8.48 compared with 0.03 ± 0.09; p < 0.001). The mean age in the study group was significantly greater than in control group (46.0 ± 12.6 compared with 38.5 ± 6.0 years; p = 0.02), but controlling for age did not affect the results. Post hoc, an optical density cutoff value of 0.3 distinguished the study group from the control group with 100% accuracy.
  
  
  CONCLUSIONS
  A novel fibronectin-aggrecan complex is present in the synovial fluid of painful knees with meniscal abnormality. The fibronectin-aggrecan complex may prove to be useful as a clinical biomarker or therapeutic target. Further research is warranted to correlate functional outcome after surgery with the fibronectin-aggrecan complex and other cartilage biomarkers.
authors.affiliation: Jupiter Outpatient Medical Center, Jupiter, Florida, USA. gscuderi@stanford.edu
authors.forename: Gaetano J
authors.initials: GJ
authors.lastname: Scuderi
authors.forename: S Raymond
authors.initials: SR
authors.lastname: Golish
authors.forename: Frank F
authors.initials: FF
authors.lastname: Cook
authors.forename: Jason M
authors.initials: JM
authors.lastname: Cuellar
authors.forename: Robert P
authors.initials: RP
authors.lastname: Bowser
authors.forename: Lewis S
authors.initials: LS
authors.lastname: Hanna
mesh_terms: D000328:Adult; D053674:Aggrecans / Q000032:analysis*; D001182:Arthroscopy; D015415:Biomarkers / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007718:Knee Injuries / Q000175:diagnosis* / Q000601:surgery*; D008297:Male; D008592:Menisci, Tibial / Q000601:surgery*; D008875:Middle Aged; D010003:Osteoarthritis / Q000175:diagnosis* / Q000601:surgery*; D010147:Pain Measurement; D011183:Postoperative Complications / Q000209:etiology; D011379:Prognosis; D013582:Synovial Fluid / Q000737:chemistry*; D000070600:Tibial Meniscus Injuries
chemical_list: D053674:Aggrecans; D015415:Biomarkers; D005353:Fibronectins