title: High-resolution melting curve analysis to establish CYP2C19∗2 single nucleotide polymorphism: Comparison with hydrolysis SNP analysis.
abstract: Clinically significant consequences of CYP2C19 polymorphism in drug metabolism require reliable genotyping methods for assaying large numbers of blood samples. Hydrolysis single nucleotide polymorphism analysis and high-resolution melting curve (HRM) analysis successfully genotyped the 114 liver donors for CYP2C19∗2. However, the HRM analysis was found to be more efficient, rapid, user- and cost-friendly method.
authors.affiliation: Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri 59-67, H-1025 Budapest, Hungary. m.temesvari@chemres.hu
authors.forename: Manna
authors.initials: M
authors.lastname: Temesvári
authors.forename: József
authors.initials: J
authors.lastname: Paulik
authors.forename: László
authors.initials: L
authors.lastname: Kóbori
authors.forename: Katalin
authors.initials: K
authors.lastname: Monostory
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism; D065731:Cytochrome P-450 CYP2C19; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D006801:Humans; D006868:Hydrolysis; D016031:Liver Transplantation; D008862:Microsomes, Liver / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D014019:Tissue Donors
chemical_list: D017931:DNA Primers; D001189:Aryl Hydrocarbon Hydroxylases; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19