title: SV40 host-substituted variants: a new look at the monkey DNA inserts and recombinant junctions.
abstract: The available monkey genomic data banks were examined in order to determine the chromosomal locations of the host DNA inserts in 8 host-substituted SV40 variant DNAs. Five of the 8 variants contained more than one linked monkey DNA insert per tandem repeat unit and in all cases but one, the 19 monkey DNA inserts in the 8 variants mapped to different locations in the monkey genome. The 50 parental DNAs (32 monkey and 18 SV40 DNA segments) which spanned the crossover and flanking regions that participated in monkey/monkey and monkey/SV40 recombinations were characterized by substantial levels of microhomology of up to 8 nucleotides in length; the parental DNAs also exhibited direct and inverted repeats at or adjacent to the crossover sequences. We discuss how the host-substituted SV40 variants arose and the nature of the recombination mechanisms involved.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA. msinger@ciw.edu
authors.forename: Maxine
authors.initials: M
authors.lastname: Singer
authors.forename: Ernest
authors.initials: E
authors.lastname: Winocour
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics*; D000882:Haplorhini; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
chemical_list: D004279:DNA, Viral