title: Identification of an mRNP complex regulating tumorigenesis at the translational elongation step.
abstract: Transcript-selective translational regulation of epithelial-mesenchymal transition (EMT) by transforming growth factor-β (TGF-β) is directed by the hnRNP E1-containing TGF-β-activated-translational (BAT) mRNP complex. Herein, eukaryotic elongation factor-1 A1 (eEF1A1) is identified as an integral component of the BAT complex. Translational silencing of Dab2 and ILEI, two EMT transcripts, is mediated by the binding of hnRNP E1 and eEF1A1 to their 3'UTR BAT element, whereby hnRNP E1 stalls translational elongation by inhibiting the release of eEF1A1 from the ribosomal A site. TGF-β-mediated hnRNP E1 phosphorylation, through Akt2, disrupts the BAT complex, thereby restoring translation of target EMT transcripts. Attenuation of hnRNP E1 expression in two noninvasive breast epithelial cells (NMuMG and MCF-7) not only induced EMT but also enabled cells to form metastatic lesions in vivo. Thus, translational regulation by TGF-β at the elongation stage represents a critical checkpoint coordinating the expression of EMT transcripts required during development and in tumorigenesis and metastatic progression.
authors.affiliation: Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
authors.forename: George S
authors.initials: GS
authors.lastname: Hussey
authors.forename: Arindam
authors.initials: A
authors.lastname: Chaudhury
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Dawson
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Lindner
authors.forename: Charlotte R
authors.initials: CR
authors.lastname: Knudsen
authors.forename: Matthew C J
authors.initials: MC
authors.lastname: Wilce
authors.forename: William C
authors.initials: WC
authors.lastname: Merrick
authors.forename: Philip H
authors.initials: PH
authors.lastname: Howe
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D015977:Eukaryotic Initiation Factor-1 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D046908:Peptide Biosynthesis, Nucleic Acid-Independent / Q000502:physiology; D010441:Peptide Chain Elongation, Translational / Q000502:physiology*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D015977:Eukaryotic Initiation Factor-1; D012261:Ribonucleoproteins; D016212:Transforming Growth Factor beta; C026452:messenger ribonucleoprotein