title: Dynamic oxidoreductive potential of astringent retraction agents.
abstract: The aim of this study was to evaluate the dynamics of the cytotoxicity of gingival margin retraction astringents based on aluminium chloride, aluminium sulphate, and ferric sulphate (solutions and gels) in human fibroblasts isolated from gingiva. The cytocompatibility of ten astringent-based chemical retraction agents: Gingiva Liquid, Alustin, Racestypine, Orbat sensitive, Astringedent®, Alustat, Hemostat, Racécord, Gel cord and ViscoStat®, in dilutions of 1 : 10 and 1 : 20, with human gingival fibroblasts was investigated. The MTT assay was performed to determine oxidoreductive mitochondrial function after 3, 5, 10 min and 24 h of incubation. Cell viability was determined according to the chemical group, concentration, exposure time, and the clinical form of the gingival retraction agents. Ferric sulphate- based agents were the most cytotoxic, followed by aluminium chloride and aluminium sulphate. The form of the astrigents influenced cell viability. The evaluated astringents may have cytotoxic potential for gingival margin tissues under clinical conditions.
authors.affiliation: Department of Dental Materials, Wroclaw Medical University, Wroclaw, Poland. michal@bioch.am.wroc.pl
authors.forename: D
authors.initials: D
authors.lastname: Nowakowska
authors.forename: J
authors.initials: J
authors.lastname: Saczko
authors.forename: J
authors.initials: J
authors.lastname: Kulbacka
authors.forename: A
authors.initials: A
authors.lastname: Choromanska
mesh_terms: D000534:Alum Compounds / Q000633:toxicity; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity; D001252:Astringents / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000633:toxicity; D004396:Coloring Agents; D005290:Ferric Compounds / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000534:Alum Compounds; D017607:Aluminum Compounds; D001252:Astringents; D002712:Chlorides; D004396:Coloring Agents; D005290:Ferric Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; C041524:aluminum sulfate; D000077410:Aluminum Chloride; C024823:ferric sulfate; C022616:thiazolyl blue