title: Effect of human beta-defensin-3 on the proliferation of fibroblasts on periodontally involved root surfaces.
abstract: Human beta-defensin-3 (HBD-3) has versatile antibacterial activity against oral bacteria and can promote the proliferation of fibroblasts. The goal of the present study was to investigate the effect of HBD-3 on attachment and proliferation of periodontal ligament cells (PDL) onto the periodontitis affected root surfaces. PDL cells were seeded onto healthy and diseased root specimens with scaling and root planing (SRP), SRP & HBD-3 (100 ng/ml), or SRP & HBD-3 (200 ng/ml) treatment for 1, 3, and 7 days incubation. The results showed that HBD-3, especially in the 200 ng/ml group, significantly promoted fibroblast attachment and proliferation onto the diseased root surfaces. The cell number in the HBD-3 group was much greater than in the group treated with SRP alone. On day 7, the cells in the HBD-3 were well-spread and formed a network similar to those on the surfaces of the healthy root specimens. These results suggest that HBD-3 could play an important role in antibacterial activity and fibroblast proliferation, thus promoting periodontal regeneration. Meanwhile, HBD-3 might act as a potent regeneration-promoter in infectious diseases.
authors.affiliation: Department of Oral Mucous Diseases, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Hisashi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ogita
authors.forename: Shizuko
authors.initials: S
authors.lastname: Ichinose
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Izumi
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D014092:Tooth Root / Q000166:cytology*; D023083:beta-Defensins / Q000494:pharmacology*
chemical_list: C422950:DEFB103A protein, human; D023083:beta-Defensins