title: Ag@poly(m-phenylenediamine) core-shell nanoparticles for highly selective, multiplex nucleic acid detection.
abstract: In this letter, we report on the one-step synthesis of Ag@poly(m-phenylenediamine) core-shell nanoparticles (APCSNPs), carried out by direct mixing of aqueous silver nitrate and m-phenylenediamine solutions at room temperature. We further demonstrate the use of APCSNP as a novel fluorescent sensing platform for nucleic acid detection. In this regard, the detection of DNA is accomplished in two steps. First, APCSNP absorbs and quenches the fluorescence of dye-labeled single-stranded DNA (ssDNA) as a probe. Second, hybridizing of the probe with its target produces a double-stranded DNA (dsDNA) that detaches from APCSNP, resulting in the recovery of dye fluorescence. It suggests that this sensing system has a high selectivity down to single-base mismatch, and the results exhibit good reproducibility. Furthermore, we also demonstrate its application for the multiplex detection of nucleic acid sequences.
authors.affiliation: State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jingqi
authors.initials: J
authors.lastname: Tian
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Luo
authors.forename: Xuping
authors.initials: X
authors.lastname: Sun
mesh_terms: D004247:DNA / Q000032:analysis*; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010655:Phenylenediamines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D010655:Phenylenediamines; C435915:poly(1,3-phenylenediamine); D012834:Silver; D004247:DNA