title: Osteogenic activity of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on irradiated allogenic bone.
abstract: Allogenic bone grafting, a technique used in orthopaedic surgery, has several problems, including low osteogenic activity. To overcome the problem, this study aimed to determine whether in vivo osteogenesis could be enhanced using allogenic irradiated bone grafts after seeding with autologous bone marrow-derived mesenchymal stem cells (BMSCs). The allogenic bone cylinders were extracted from ACI rats and sterilized by irradiation. Donor BMSCs were obtained from fresh Fischer 344 (F344) rat bone marrow by cell culture. The allogenic bone with or without BMSCs were transplanted subcutaneously into syngeneic F344 rats. At 4 weeks after transplantation, high alkaline phosphatase (ALP) activity, bone-specific osteocalcin mRNA expression and newly formed bone were detected in the allogenic bone with BMSCs. The origin of the newly formed bone was derived from cultured donor BMSCs. However, none of these identifiers of osteogenesis were detected in either the fresh or the irradiated allogenic bone without BMSCs. These results indicate the availability of autologous BMSCs to heighten the osteogenic response of allogenic bone. Our present tissue-engineering method might contribute to a wide variety of allogenic bone grafting techniques in clinical settings.
authors.affiliation: Department of Orthopaedic Surgery, Nara Medical University, Nara, Japan. SNC45147@nifty.com
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tohma
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Dohi
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
authors.forename: Mika
authors.initials: M
authors.lastname: Tadokoro
authors.forename: Manabu
authors.initials: M
authors.lastname: Akahane
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D016025:Bone Transplantation; D001842:Bone and Bones / Q000201:enzymology / Q000528:radiation effects*; D005260:Female; D005786:Gene Expression Regulation / Q000528:radiation effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology* / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014184:Transplantation, Homologous; D014965:X-Rays; D014998:Y Chromosome / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D015675:Osteocalcin; D000469:Alkaline Phosphatase