title: Isolation and characterisation of KP34--a novel φKMV-like bacteriophage for Klebsiella pneumoniae.
abstract: Bacteriophage KP34 is a novel virus belonging to the subfamily Autographivirinae lytic for extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains. Its biological features, morphology, susceptibility to chemical and physical agents, burst size, host specificity and activity spectrum were determined. As a potential antibacterial agent used in therapy, KP34 molecular features including genome sequence and protein composition were examined. Phylogenetic analyses and clustering of KP34 phage genome sequences revealed its clear relationships with "phiKMV-like viruses". Simultaneously, whole-genome analyses permitted clustering and classification of all phages, with completely sequenced genomes, belonging to the Podoviridae.
authors.affiliation: Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland. zuzanna.drulis-kawa@microb.uni.wroc.pl
authors.forename: Zuzanna
authors.initials: Z
authors.lastname: Drulis-Kawa
authors.forename: Paweł
authors.initials: P
authors.lastname: Mackiewicz
authors.forename: Agata
authors.initials: A
authors.lastname: Kęsik-Szeloch
authors.forename: Ewa
authors.initials: E
authors.lastname: Maciaszczyk-Dziubinska
authors.forename: Beata
authors.initials: B
authors.lastname: Weber-Dąbrowska
authors.forename: Agata
authors.initials: A
authors.lastname: Dorotkiewicz-Jach
authors.forename: Daria
authors.initials: D
authors.lastname: Augustyniak
authors.forename: Grażyna
authors.initials: G
authors.lastname: Majkowska-Skrobek
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bocer
authors.forename: Joanna
authors.initials: J
authors.lastname: Empel
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016679:Genome, Viral; D058507:Host Specificity; D007711:Klebsiella pneumoniae / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012722:Sewage / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012722:Sewage; D014764:Viral Proteins