title: Formosanin C-inhibited pulmonary metastasis through repression of matrix metalloproteinases on mouse lung adenocarcinoma.
abstract: Formosanin C (FC) isolated from Rhizoma Paridis, showed pro-apoptosis and immunoregulation with antitumor activity in cultured cells and animal systems. However, there is no report about its anti-metastatic effect on cancer cells. This research used the wound healing and the migration assay to detect the anti-invasive effect of FC on LA795 cells. Through the gelatin zymography assay and immunofluorescence analysis, FC showed suppression of enzyme activity of MMP-2 and MMP-9, and protein expression of MMP-1, -2, -3, -9 and -14 excreted from LA795 cells. Finally, FC exhibited much more effective inhibition of tumor growth and pulmonary metastasis in T739 mice than cisplatin did. Overall, the strong inhibition of MMP expression by FC might provide a potential therapeutic modality for lung tumors.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Man
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Lulu
authors.initials: L
authors.lastname: Yan
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
authors.forename: Changxiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000517:prevention & control* / Q000556:secondary*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002945:Cisplatin / Q000627:therapeutic use; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D051379:Mice; D008815:Mice, Inbred Strains; D009362:Neoplasm Metastasis; D012503:Saponins / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D061965:Matrix Metalloproteinase Inhibitors; D012503:Saponins; C067584:formosanin C; D004144:Diosgenin; D002945:Cisplatin