title: [Effect of HBc-antigen expression on replication of recombinant Salmonella strains in vivo and in vitro].
abstract: The development of therapeutic vaccines against chronic hepatitis B is a major challenge facing modern medicine. HBc antigen-expressing DNA-vaccines designed to be transported into the organism by attenuated Salmonella appear to be good candidates for the purpose. Expression of HBc-antigen was earlier shown to lead to the loss of ability of attenuated Salmonella bearing the DNA-vaccine to multiply in the host lymphoid tissue of mice after per rectum immunization and in human monocyte culture. The aim of the study was to assess the influence of HBc-antigen expression on replication of a virulent strain of wild-type Salmonella serotype Enteritidis in the murine lymphoid tissue after per rectum infection and in human blood monocytes. HBc expression was shown to inhibit propagation of Salmonella in both model systems despite its high virulence.
authors.forename: M N
authors.initials: MN
authors.lastname: Boĭchenko
authors.forename: M V
authors.initials: MV
authors.lastname: Donin
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
mesh_terms: D000818:Animals; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis*; D051379:Mice; D012475:Salmonella / Q000254:growth & development*; D012480:Salmonella Infections / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology*; D019444:Vaccines, DNA / Q000494:pharmacology; D014774:Virulence
chemical_list: D006512:Hepatitis B Core Antigens; D019444:Vaccines, DNA