title: Two-photon excited hemoglobin fluorescence.
abstract: We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
authors.forename: Jianan Y
authors.initials: JY
authors.lastname: Qu