title: Proteomic identification of radiation response markers in mouse intestine and brain.
abstract: Increasing efforts are being made to develop more sensitive and faster molecular methodologies at the genomic and proteomic levels for the identification of protein markers after exposure to ionizing radiation (IR). However, few specific protein markers, especially organ-specific markers, have been identified. In this study, we analyzed altered protein expressions in various tissues, namely, brain, lung, spleen, and intestine, from 1  Gy-irradiated mice by employing 2-DE analysis. MALDI-TOF MS and peptide mapping identified 25 proteins that showed greater than twofold expressional changes. In order to confirm significant differences between control and IR-treated samples, ten identified proteins with available commercial antibodies were selected for immunoblotting. Of these, only five showed protein expression patterns that were similar to 2-DE data. These were heat shock protein 5 (HSP 5), HSP 90  kDa β, HSP 1, transaldolase 1 (TA1), and phosphoglycerate kinase 1 (PGK1). In particular, PGK1 was specifically upregulated in mouse intestine, and TA1 was specifically downregulated in brain by irradiation. TA1 expression was unaltered in other tissues. Based on these data, we suggest that TA1 and PGK1 can be considered as candidate tissue-specific protein markers of IR exposure.
authors.affiliation: Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
authors.forename: Young-Bin
authors.initials: YB
authors.lastname: Lim
authors.forename: Bo-Jeong
authors.initials: BJ
authors.lastname: Pyun
authors.forename: Hae-June
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sang-Rok
authors.initials: SR
authors.lastname: Jeon
authors.forename: Yeung Bae
authors.initials: YB
authors.lastname: Jin
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D007422:Intestines / Q000737:chemistry / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D010449:Peptide Mapping; D010735:Phosphoglycerate Kinase / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D011839:Radiation, Ionizing; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014153:Transaldolase / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: D015415:Biomarkers; D020543:Proteome; D014153:Transaldolase; C528165:Pgk1 protein, mouse; D010735:Phosphoglycerate Kinase