title: Th1/Th2 cytometric bead array can discriminate cytokine secretion from endogenously activated cells in pulmonary disease, recent and remote infection in tuberculosis.
abstract: Differential T cell trafficking through the blood compartment towards infected foci may be occurring in different stages of tuberculosis disease and infection. The aim of the present study was to identify cytokine signatures in the blood compartment in tuberculosis patients with pulmonary disease (PTB=19), recently exposed household contacts (HC=27) and nonexposed community controls (EC=37). Diluted (1:10) whole blood was cultured for 2 days and cytokine secretion was assessed using Cytometric Bead Array (Th1/Th2 kit II; BD Biosciences) which included IL-2, TNF-α, IFN-γ (Type1/T1), IL-4, IL-6 and IL-10 (Type2/T2). All T1/T2 cytokines were elevated in PTB (AUROC>0.9) while HC showed selective elevation of IL-6 (AUROC>0.7) compared to EC. Principal component analysis (PCA) extracted two groupings with Eigen values >1; IL-6 separated into the second component for PTB, HC and EC. After rotation, IFN-γ was correlated with the first component for PTB and EC and the second component for HC indicating an absence of T1/T2 dichotomy. Therefore endogenous cytokine signatures may indicate differential T cell trafficking in different stages of tuberculosis infection and disease.
authors.affiliation: Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan.
authors.forename: Najeeha
authors.initials: N
authors.lastname: Talat
authors.forename: Firdaus
authors.initials: F
authors.lastname: Shahid
authors.forename: Sharon
authors.initials: S
authors.lastname: Perry
authors.forename: Ghaffar
authors.initials: G
authors.lastname: Dawood
authors.forename: Rabia
authors.initials: R
authors.lastname: Hussain
mesh_terms: D015331:Cohort Studies; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000276:immunology*; D008297:Male; D025341:Principal Component Analysis; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D016207:Cytokines