title: Assessing a novel room temperature DNA storage medium for forensic biological samples.
abstract: The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells and the DNA extracts from them is particularly important since testing is generally not performed immediately following collection. Furthermore, retesting of stored DNA samples may be needed in casework for replicate testing, confirmation of results, and to accommodate future testing with new technologies. A novel room temperature DNA storage medium, SampleMatrix™ (SM; Biomatrica, Inc., San Diego, CA), was evaluated for stabilizing and protecting samples. Human genomic DNA samples at varying amounts (0.0625-200 ng) were stored dry in SM for 1 day to 1 year under varying conditions that included a typical ambient laboratory environment and also through successive freeze-thaw cycles (3 cycles). In addition, spiking of 1-4 × SM into samples prior to analysis was performed to determine any inhibitory effects of SM. Quantification of recovered DNA following storage was determined by quantitative PCR or by agarose gel electrophoresis, and evaluation of quantitative peak height results from multiplex short tandem repeat (STR) analyses were performed to assess the efficacy of SM for preserving DNA. Results indicate no substantial differences between the quality of samples stored frozen in liquid and those samples maintained dry at ambient temperatures protected in SM. For long-term storage and the storage of low concentration samples, SM provided a significant advantage over freezer storage through higher DNA recovery. No detectable inhibition of amplification was observed at the recommended SM concentration and complete profiles were obtained from genomic DNA samples even in the presence of higher than recommended concentrations of the SM storage medium. The ability to stabilize and protect DNA from degradation at ambient temperatures for extended time periods could have tremendous impact in simplifying and improving sample storage conditions and requirements. The current work focuses on forensics analysis; however this technology is applicable to all endeavors requiring storage of DNA.
authors.affiliation: Forensic Science Program, Justice Studies Department, MacQuarrie Hall 521, San Jose State University, San Jose, CA 95192, USA. sblee999@gmail.com
authors.forename: Steven B
authors.initials: SB
authors.lastname: Lee
authors.forename: Kimberly C
authors.initials: KC
authors.lastname: Clabaugh
authors.forename: Brie
authors.initials: B
authors.lastname: Silva
authors.forename: Kingsley O
authors.initials: KO
authors.lastname: Odigie
authors.forename: Michael D
authors.initials: MD
authors.lastname: Coble
authors.forename: Odile
authors.initials: O
authors.lastname: Loreille
authors.forename: Melissa
authors.initials: M
authors.lastname: Scheible
authors.forename: Ron M
authors.initials: RM
authors.lastname: Fourney
authors.forename: Jesse
authors.initials: J
authors.lastname: Stevens
authors.forename: George R
authors.initials: GR
authors.lastname: Carmody
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Parsons
authors.forename: Arijana
authors.initials: A
authors.lastname: Pozder
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Eisenberg
authors.forename: Bruce
authors.initials: B
authors.lastname: Budowle
authors.forename: Taha
authors.initials: T
authors.lastname: Ahmad
authors.forename: Russell W
authors.initials: RW
authors.lastname: Miller
authors.forename: Cecelia A
authors.initials: CA
authors.lastname: Crouse
mesh_terms: D004247:DNA / Q000737:chemistry*; D053803:Forensic Genetics; D006801:Humans; D013048:Specimen Handling; D013696:Temperature
chemical_list: D004247:DNA