title: Methadone determination in puparia and its effect on the development of Lucilia sericata (Diptera, Calliphoridae).
abstract: This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in puparia reared on high concentrated substrates (0.8-4 μg/g). The major metabolite of methadone (EDDP) was not detected, confirming rapid elimination of metabolites by the larvae before pupation. The effects of methadone on the development of L. sericata were also investigated. No effect on sex ratio was detected. A significant difference was calculated for emerged adults but no trends could be observed. Concerning the developmental curve, a significant difference was observed between control and high methadone concentrations using the Kolmogorov-Smirnov test.
authors.affiliation: Laboratory of Toxicology, National Institute of Criminalistics and Criminology, Brussels, Belgium. Matthias.gosselin@just.fgov.be
authors.forename: Matthias
authors.initials: M
authors.lastname: Gosselin
authors.forename: Vincent
authors.initials: V
authors.lastname: Di Fazio
authors.forename: Sarah M R
authors.initials: SM
authors.lastname: Wille
authors.forename: Maria del Mar Ramírez
authors.initials: Mdel M
authors.lastname: Fernandez
authors.forename: Nele
authors.initials: N
authors.lastname: Samyn
authors.forename: Benoit
authors.initials: B
authors.lastname: Bourel
authors.forename: Pierre
authors.initials: P
authors.lastname: Rasmont
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004175:Diptera / Q000187:drug effects / Q000254:growth & development*; D005260:Female; D053593:Forensic Toxicology; D007814:Larva; D008297:Male; D013058:Mass Spectrometry; D008691:Methadone / Q000032:analysis* / Q000633:toxicity*; D009294:Narcotics / Q000032:analysis* / Q000633:toxicity*; D011679:Pupa / Q000187:drug effects / Q000254:growth & development; D011759:Pyrrolidines / Q000032:analysis
chemical_list: D009294:Narcotics; D011759:Pyrrolidines; D008691:Methadone; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine