title: CIDEA interacts with liver X receptors in white fat cells.
abstract: Cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA) is endogenously expressed in human but not rodent white adipocytes. We performed a bioinformatic analysis of the human CIDEA sequence and found conserved amino-acid motifs involved in binding to nuclear receptors. Protein-protein binding experiments and transactivation assays confirmed that CIDEA binds to liver X receptors and regulates their activity in vitro. Cell fractionation demonstrated that CIDEA localizes to both the cytoplasm and the nucleus in human white adipocytes. The interaction between CIDEA and nuclear receptors could therefore be of importance for the regulation of metabolic processes in human adipose tissue.
authors.affiliation: Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
authors.forename: Agné
authors.initials: A
authors.lastname: Kulyté
authors.forename: Amanda T
authors.initials: AT
authors.lastname: Pettersson
authors.forename: Per
authors.initials: P
authors.lastname: Antonson
authors.forename: Britta M
authors.initials: BM
authors.lastname: Stenson
authors.forename: Dominique
authors.initials: D
authors.lastname: Langin
authors.forename: Jan-Ake
authors.initials: JA
authors.lastname: Gustafsson
authors.forename: Bart
authors.initials: B
authors.lastname: Staels
authors.forename: Mikael
authors.initials: M
authors.lastname: Rydén
authors.forename: Peter
authors.initials: P
authors.lastname: Arner
authors.forename: Jurga
authors.initials: J
authors.lastname: Laurencikiene
mesh_terms: D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D000071518:Liver X Receptors; D051379:Mice; D008969:Molecular Sequence Data; D057093:Orphan Nuclear Receptors / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D051017:Apoptosis Regulatory Proteins; C502539:CIDEA protein, human; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors