title: Effects of acute oral administration of vitamin C on the mouse liver transcriptome.
abstract: Vitamin C is a strong antioxidant that alters gene expression in cells, and its effects can be modified by cellular oxidative stress. We investigated the genome-wide effects of vitamin C on the in vivo transcriptome in the liver, which synthesizes various enzymes and proteins to defend against cellular oxidative stress. We fed mice vitamin C (0.056 mg/g of body weight) for 1 week and performed DNA microarray analysis with hepatic mRNA in fasting and refeeding states to mimic physiological conditions of oxidative stress. Significance analysis of microarray data identified approximately 6,000 genes differentially expressed in both fasting and refeeding states. In the fasting state, vitamin C induced overall energy metabolism as well as radical scavenging pathways. These were ameliorated in the refeeding state. These findings suggest that vitamin C has profound and immediate global effects on hepatic gene expression, which may help prevent oxidative stress, and that long-term treatment with vitamin C might reduce the risk of chronic disease.
authors.affiliation: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Jun
authors.forename: Sukyung
authors.initials: S
authors.lastname: Kim
authors.forename: Kevin
authors.initials: K
authors.lastname: Dawson
authors.forename: Dal-Woong
authors.initials: DW
authors.lastname: Choi
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Raymond L
authors.initials: RL
authors.lastname: Rodriguez
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005215:Fasting; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling / Q000379:methods; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D001205:Ascorbic Acid