title: Trypanosoma evansi: a comparative study of four diagnostic techniques for trypanosomosis using rabbit as an experimental model.
abstract: The goal of this study was to compare two parasitological diagnostic techniques, such as by Micro-Haematocrit Centrifugation Technique (MHCT) and Direct Microscopic Examination (DME) with a serological method (iELISA), and a molecular procedure PCR, in rabbits experimentally infected with Trypanosoma evansi, in order to determine their sensitivity throughout the course of disease. The parasitological methods were not able of detecting the presence of the parasite during the phases of low parasitemia, the prepatency period and the chronic phase. In contrast, PCR detected T. evansi in the prepatency and chronic phase, when increase the amount of DNA from 100 to 300ng. 100% detection was observed with iELISA only in the chronic stage of the disease. In the acute phase, all samples were positively diagnosed using either MHCT or PCR, whereas only few samples were diagnosed by DME. Samples obtained from day 15 post infection were also detected by iELISA. The highest diagnostic register during the course of infection was achieved by the PCR technique (93.8%), followed by iELISA (71.1%), MHCT (59%) and DME (13.6%). Therefore, we recommend the use of PCR in epidemiological studies in order to implement sanitary control plans for the improvement of livestock productivity in the country.
authors.affiliation: Universidad Nacional Experimental Simón Rodríguez, Instituto de Estudios Científicos y Tecnológicos, Centro de Estudios Biomédicos y Veterinarios, Caracas, Venezuela.
authors.forename: J R
authors.initials: JR
authors.lastname: Ramírez-Iglesias
authors.forename: M C
authors.initials: MC
authors.lastname: Eleizalde
authors.forename: E
authors.initials: E
authors.lastname: Gómez-Piñeres
authors.forename: M
authors.initials: M
authors.lastname: Mendoza
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001769:Blood / Q000469:parasitology; D002498:Centrifugation; D002908:Chronic Disease; D016054:DNA, Protozoan / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016133:Polymerase Chain Reaction; D011817:Rabbits; D051381:Rats; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan