title: DNA-functionalized nanochannels for SNP detection.
abstract: We have developed ultrahigh density array of functionalized nanochannels by using a block copolymer having end di-COOH group. This approach provides a facile route for direct functionalization of wall surface of the nanochannels and immobilization site for molecular recognition agents (MRAs). By using overhanging single-stranded DNA as MRAs, the DNA-functionalized nanochannels showed high resolution to detect a single-base mismatch as well as to discriminate single-mismatched sequence at various locations by hybridization preference with MRAs.
authors.affiliation: National Creative Research Center for Block Copolymer Self-Assembly, Department of Chemical Engineering, Pohang University of Science and Technology , Kyungbuk 790-784, Korea.
authors.forename: Seung Yun
authors.initials: SY
authors.lastname: Yang
authors.forename: Sejin
authors.initials: S
authors.lastname: Son
authors.forename: Sangsin
authors.initials: S
authors.lastname: Jang
authors.forename: Hyunwoo
authors.initials: H
authors.lastname: Kim
authors.forename: Gumhye
authors.initials: G
authors.lastname: Jeon
authors.forename: Won Jong
authors.initials: WJ
authors.lastname: Kim
authors.forename: Jin Kon
authors.initials: JK
authors.lastname: Kim
mesh_terms: D004247:DNA / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D020641:Polymorphism, Single Nucleotide
chemical_list: D004247:DNA