title: A calcium-cross-linked hydrogel based on alginate-modified atelocollagen functions as a scaffold material.
abstract: We have developed a scaffold material consisting of a covalently-bonded structure of alginate and atelocollagen (AtCol). Addition of calcium ions caused the material to form a hydrogel (alginate-modified AtCol gel). The condition of the alginate-modified AtCol gel could be controlled  by the feed ratio of alginate and the activating reagent. Measurement of temporal stability in culture medium suggested that covalent bonding between alginate and AtCol might contribute to the structural stability of the alginate-modified AtCol gel. Culture with endothelial cells indicated  that cell adhesiveness on the alginate-modified AtCol gel was similar to that on native collagen. Collagenase digestion revealed that the alginate-modified AtCol gel had considerable ability to retain basic fibroblast growth factor. Additionally, active cell migration into alginate-modified  AtCol was detected by in vitro assay using endothelial cells. These findings indicate that this gel material can be expected to function as a scaffold for inducing vascular in-growth.
authors.affiliation: Department of Vascular Regeneration, Division of Tissue Engineering, The University of Tokyo Hospital, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Kamimura
authors.forename: Rieko
authors.initials: R
authors.lastname: Hattori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koyama
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miyata
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002122:Calcium Chloride / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology; D004952:Esters; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D004952:Esters; D006603:Hexuronic Acids; D020100:Hydrogels; D013388:Succinimides; C059821:atelocollagen; D016222:Fibroblast Growth Factor 2; C032620:succinimide; D020723:Glucuronic Acid; D003094:Collagen; D002122:Calcium Chloride; D002118:Calcium