title: [Relationship between the reaching of equilibrium and the accuracy of the affinity determination for antibody-antigen reaction].
abstract: Theoretical investigation of the relationship between the reaching of equilibrium and the accuracy of the affinity determination for antibody-antigen reaction were carried out. It was shown that evaluation of the reagent concentrations before reaching the dynamic equilibrium leads to a decrease of estimation accuracy for the affinity constant of this reaction. We have established that for most of typical antibodies the dynamic equilibrium of their reaction with corresponding antigens comes 2-4 hours after the beginning.
authors.forename: S A
authors.initials: SA
authors.lastname: Bobrovnik
authors.forename: M A
authors.initials: MA
authors.lastname: Demchenko
authors.forename: S V
authors.initials: SV
authors.lastname: Komisarenko
mesh_terms: D000915:Antibody Affinity; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D007700:Kinetics; D018448:Models, Immunological
chemical_list: D000936:Antigen-Antibody Complex