title: Chemosynthesis of poly(ε-lysine)-analogous polymers by microwave-assisted click polymerization.
abstract: Poly(ε-lysine) (ε-PL)-analogous click polypeptides with not only similar α-amino side groups but also similar main chain to ε-PL were chemically synthesized for the first time through click polymerization from aspartic (or glutamic)-acid-based dialkyne and diazide monomers. With microwave-assisting, the reaction time of click polymerization was compressed into 30 min. The polymers were fully characterized by NMR, ATR-FTIR, and SEC-MALLS analysis. The deprotected click polypeptides had similar pK(a) value (7.5) and relatively low cytotoxicity as ε-PL and could be used as substitutes of ε-PL in biomedical applications, especially in endotoxin selective removal. Poly(ethylene glycol) (PEG)-containing alternating copolymers with α-amino groups were also synthesized and characterized. After deprotection, the polymers could be used as functional gene vector with PEG shadowing system and NCA initiator to get amphiphilic graft polymers.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Renmin Str. 5625, Changchun 130022, P. R. China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
authors.forename: Dongfang
authors.initials: D
authors.lastname: Zhou
authors.forename: Pingqiang
authors.initials: P
authors.lastname: Cai
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
authors.forename: Xue-Si
authors.initials: XS
authors.lastname: Chen
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000596:Amino Acids; D004731:Endotoxins / Q000302:isolation & purification; D006801:Humans; D008872:Microwaves; D011092:Polyethylene Glycols; D011107:Polylysine / Q000138:chemical synthesis* / Q000627:therapeutic use; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000627:therapeutic use
chemical_list: D000596:Amino Acids; D004731:Endotoxins; D011108:Polymers; D011107:Polylysine; D011092:Polyethylene Glycols