title: Cellular prion proteins in human platelets show a phenotype different to those in brain tissues.
abstract: Prion diseases are characterized by high accumulation of infectious prion proteins (PrP(Sc)) in brains. PrP(Sc) are propagated by the conversion of host-encoded cellular prion proteins (PrP(C)) which are essential for developing the disease but are heterogeneously expressed in brains. The disease can be transmitted to humans and animals through blood and blood products, however, little attention has been given to molecular characterization of PrP(C) in blood cells. In this presented study, we characterized phenotypically PrP(C) of platelets (plt) and characterized the proteins regarding their glycobanding profiles by quantitative immunoblotting using a panel of monoclonal antibodies. The glycosylation patterns of plt and brain PrP(C) were compared using the ratios of di-, mono-, and non-glycosylated prions. The detergent solubility of plt and brain PrP(C) was also analyzed. The distinct banding patterns and detergent solubility of plt PrP(C) differed clearly from the glycosylation profiles and solubility characteristics of brain PrP(C). Plt PrP(C) exhibited single or only few prion protein types, whereas brain PrP(C) showed more extensive banding patterns and lower detergent solubility. Plt PrP(C) are post-translational modified differently from PrP(C) in brain. These findings suggest other or less physiological functions of plt PrP(C) than in brain.
authors.affiliation: Institute for Hygiene, Westfälische Wilhelms-University and University Hospital Münster, Robert Koch-Strasse 41, 48149 Münster, Germany. tkuczius@uni-muenster.de
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kuczius
authors.forename: Julia
authors.initials: J
authors.lastname: Kleinert
authors.forename: Helge
authors.initials: H
authors.lastname: Karch
authors.forename: Walter
authors.initials: W
authors.lastname: Sibrowski
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kelsch
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D010641:Phenotype; D011328:Prions / Q000737:chemistry / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012995:Solubility
chemical_list: D011328:Prions; D020033:Protein Isoforms