title: Properties of partially purified endopolyphosphatase of the yeast Saccharomyces cerevisiae.
abstract: Partially purified endopolyphosphatase from cytosol of the yeast Saccharomyces cerevisiae with inactivated genes PPX1 and PPN1 encoding exopolyphosphatases was obtained with ion-exchange and affinity chromatography. The enzyme activity was estimated by decrease of polyphosphate chain length determined by PAGE. The enzyme cleaved inorganic polyphosphate without the release of orthophosphate (P(i)) and was inhibited by heparin and insensitive to fluoride. Mg2+, Mn2+, and Co2+ (1.5 mM) stimulated the activity, and Ca2+ was ineffective. The molecular mass of the endopolyphosphatase determined by gel filtration was of ~20 kDa.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia. alla@ibpm.pushchino.ru
authors.forename: L P
authors.initials: LP
authors.lastname: Lichko
authors.forename: T V
authors.initials: TV
authors.lastname: Kulakovskaya
authors.forename: I S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D002118:Calcium / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D057075:Enzyme Assays; D006493:Heparin / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008970:Molecular Weight; D011122:Polyphosphates / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D011122:Polyphosphates; D003035:Cobalt; D008345:Manganese; D006493:Heparin; D017766:Acid Anhydride Hydrolases; C022563:endopolyphosphatase; D008274:Magnesium; D002118:Calcium