title: Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis.
abstract: Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primiparous ewes using two S. aureus strains, isolated from gangrenous (strain O11) or subclinical (strain O46) mastitis. Strains induced drastically distinct clinical symptoms when tested in ewe and mice experimental mastitis. Notably, they reproduced mild (O46) or severe (O11) mastitis in ewes. Ewe sera were used to identify staphylococcal immunoreactive proteins commonly or differentially produced during infections of variable severity and to define core and accessory seroproteomes. Such SERological Proteome Analysis (SERPA) allowed the identification of 89 immunoreactive proteins, of which only 52 (58.4%) were previously identified as immunogenic proteins in other staphylococcal infections. Among the 89 proteins identified, 74 appear to constitute the core seroproteome. Among the 15 remaining proteins defining the accessory seroproteome, 12 were specific for strain O11, 3 were specific for O46. Distribution of one protein specific for each mastitis severity was investigated in ten other strains isolated from subclinical or clinical mastitis. We report here for the first time the identification of staphylococcal immunogenic proteins common or specific to S. aureus strains responsible for mild or severe mastitis. These findings open avenues in S. aureus mastitis studies as some of these proteins, expressed in vivo, are likely to account for the success of S. aureus as a pathogen of the ruminant mammary gland.
authors.affiliation: INRA, UMR1253 Science et Technologie du Lait et de l'Œuf, F-35042 Rennes, France. Yves.LeLoir@rennes.inra.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: Le Maréchal
authors.forename: Julien
authors.initials: J
authors.lastname: Jardin
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Jan
authors.forename: Sergine
authors.initials: S
authors.lastname: Even
authors.forename: Coralie
authors.initials: C
authors.lastname: Pulido
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Guibert
authors.forename: David
authors.initials: D
authors.lastname: Hernandez
authors.forename: Patrice
authors.initials: P
authors.lastname: François
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.forename: Dieter
authors.initials: D
authors.lastname: Demon
authors.forename: Evelyne
authors.initials: E
authors.lastname: Meyer
authors.forename: Nadia
authors.initials: N
authors.lastname: Berkova
authors.forename: Richard
authors.initials: R
authors.lastname: Thiéry
authors.forename: Eric
authors.initials: E
authors.lastname: Vautor
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D002853:Chromatography, Liquid / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D008321:Mammary Glands, Animal / Q000276:immunology / Q000382:microbiology; D008413:Mastitis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D020543:Proteome / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012698:Serologic Tests / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000662:veterinary
chemical_list: D001426:Bacterial Proteins; D020543:Proteome