title: Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU).
abstract: Acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13TU are increasingly recognized as clinically important taxa within the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex. To define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the ACB complex. All strains were characterized by AFLP analysis, amplified rDNA restriction analysis and nutritional or physiological testing, while selected strains were studied by 16S rRNA and rpoB gene sequence analysis, multilocus sequence analysis and whole-genome comparison. Results supported the genomic distinctness and monophyly of the individual species of the ACB complex. Despite the high phenotypic similarity among these species, some degree of differentiation between them could be made on the basis of growth at different temperatures and of assimilation of malonate, l-tartrate levulinate or citraconate. Considering the medical relevance of gen. sp. 3 and gen. sp. 13TU, we propose the formal names Acinetobacter pittii sp. nov. and Acinetobacter nosocomialis sp. nov. for these taxa, respectively. The type strain of A. pittii sp. nov. is LMG 1035(T) (=CIP 70.29(T)) and that of A. nosocomialis sp. nov. is LMG 10619(T) (=CCM 7791(T)).
authors.affiliation: Laboratory of Bacterial Genetics, National Institute of Public Health, Šrobárova 48, 100 42 Prague, Czech Republic. anemec@szu.cz
authors.forename: Alexandr
authors.initials: A
authors.lastname: Nemec
authors.forename: Lenka
authors.initials: L
authors.lastname: Krizova
authors.forename: Martina
authors.initials: M
authors.lastname: Maixnerova
authors.forename: Tanny J K
authors.initials: TJ
authors.lastname: van der Reijden
authors.forename: Pieter
authors.initials: P
authors.lastname: Deschaght
authors.forename: Virginie
authors.initials: V
authors.lastname: Passet
authors.forename: Mario
authors.initials: M
authors.lastname: Vaneechoutte
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
authors.forename: Lenie
authors.initials: L
authors.lastname: Dijkshoorn
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D040981:Acinetobacter baumannii / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S