title: Vasodilatory effect of hydroxyethyl methacrylate and triethylene glycol dimethacrylate in rat aorta through calcium antagonistic action.
abstract: BACKGROUND
  Resin-based dental materials contain various diluent monomers that can interfere with vascular function by causing vasodilation. In this study, we evaluated the vasoactive potential of hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) and the possible mechanism of their vascular action on isolated rat aorta.
  
  
  METHODS
  Responses of thoracic aorta rings were recorded isometrically by using force displacement transducers. After precontracting aorta rings with phenylephrine, relaxations to HEMA and TEGDMA were recorded in the absence and presence of nitric oxide synthase inhibitor N(ω)-nitro-L-arginine methyl ester, cyclooxygenase inhibitor indomethacin, and K(+) channel inhibitors tetraethylammonium, glibenclamide, and 4-aminopyridine. To investigate the Ca(2+)-channel antagonistic effect of HEMA and TEGDMA in different aorta rings, concentration-response curves to CaCl(2) were obtained in the absence and presence of the test monomers.
  
  
  RESULTS
  Both HEMA and TEGDMA elicited concentration-dependent relaxations. The vasorelaxant effect of HEMA and TEGDMA was not mediated via endothelium-dependent nitric oxide and prostanoid-dependent mechanisms or by K(+) efflux through K(+) channels. Both monomers significantly inhibited the contractions induced by CaCl(2).
  
  
  CONCLUSIONS
  Our results showed that HEMA and TEGDMA induce vasodilation via Ca(2+)-antagonistic action, whereas nitric oxide and cyclooxgenase pathway and K(+) channels were not responsible for this vasoactive effect.
authors.affiliation: Department of Pediatric Dentistry, Gulhane School of Medicine, Ankara, Turkey.
authors.forename: Gunseli
authors.initials: G
authors.lastname: Guven
authors.forename: Melik
authors.initials: M
authors.lastname: Seyrek
authors.forename: Ismail Mert
authors.initials: IM
authors.lastname: Vural
authors.forename: Zafer C
authors.initials: ZC
authors.lastname: Cehreli
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Yildiz
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D002118:Calcium / Q000037:antagonists & inhibitors*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D003188:Composite Resins / Q000494:pharmacology*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D003764:Dental Materials / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008689:Methacrylates / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000494:pharmacology*; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019789:Tetraethylammonium / Q000494:pharmacology; D014159:Transducers; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D003188:Composite Resins; D016861:Cyclooxygenase Inhibitors; D003764:Dental Materials; D004791:Enzyme Inhibitors; D008689:Methacrylates; D011109:Polymethacrylic Acids; D026902:Potassium Channel Blockers; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D019789:Tetraethylammonium; C005044:hydroxyethyl methacrylate; D015761:4-Aminopyridine; D019001:Nitric Oxide Synthase; D009543:Nifedipine; D002122:Calcium Chloride; D005905:Glyburide; D002118:Calcium; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin