title: Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli.
abstract: The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 ± 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-α (TNF-α) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3 °C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.
authors.affiliation: Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group, Ghent University, B-9000 Ghent, Belgium. Christian.Burvenich@UGent.be.
authors.forename: Adel
authors.initials: A
authors.lastname: Pezeshki
authors.forename: Philippe
authors.initials: P
authors.lastname: Stordeur
authors.forename: Hugues
authors.initials: H
authors.lastname: Wallemacq
authors.forename: Frédéric
authors.initials: F
authors.lastname: Schynts
authors.forename: Mieke
authors.initials: M
authors.lastname: Stevens
authors.forename: Philippe
authors.initials: P
authors.lastname: Boutet
authors.forename: Luc J
authors.initials: LJ
authors.lastname: Peelman
authors.forename: Bart
authors.initials: B
authors.lastname: De Spiegeleer
authors.forename: Luc
authors.initials: L
authors.lastname: Duchateau
authors.forename: Fabrice
authors.initials: F
authors.lastname: Bureau
authors.forename: Christian
authors.initials: C
authors.lastname: Burvenich
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D015777:Eicosanoids / Q000097:blood / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000276:immunology* / Q000382:microbiology; D008892:Milk / Q000737:chemistry / Q000382:microbiology; D010298:Parity; D013696:Temperature; D013817:Thermography / Q000379:methods*
chemical_list: D016207:Cytokines; D015777:Eicosanoids