title: Evaluation of methods of determining humic acids in nucleic acid samples for molecular biological analysis.
abstract: It is important in molecular biological analyses to evaluate contamination of co-extracted humic acids in DNA/RNA extracted from soil. We compared the sensitivity of various methods for measurement of humic acids, and influences of DNA/RNA and proteins on the measurement. Considering the results, we give suggestions as to choice of methods for measurement of humic acids in molecular biological analyses.
authors.affiliation: National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujii
mesh_terms: D016477:Artifacts; D006812:Humic Substances / Q000032:analysis*; D008967:Molecular Biology / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012987:Soil / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006812:Humic Substances; D009696:Nucleic Acids; D012987:Soil