title: Expression of the human cancer/testis antigen NY-SAR-35 is activated by CpG island hypomethylation.
abstract: The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various cancer cell lines showed that its expression was related to methylation of the promoter region. Treatment of human cancer cell lines with the demethylating agent 5-aza-2'-deoxycytidine activated the expression of the NY-SAR-35 gene. In addition, transfection experiments on various fragments of the CpG-rich gene promoter indicate that in vitro methylation of the NY-SAR-35 gene promoter results in the loss of promoter activity. The expression of NY-SAR-35 is therefore activated by hypomethylation of the CpG island in the gene promoter.
authors.affiliation: Department of Biochemistry, School of Medicine, Pusan National University, Beomeori, Mulgeumeup, Yangsan, 626-770, Republic of Korea.
authors.forename: Jin-Hyung
authors.initials: JH
authors.lastname: Park
authors.forename: Myung-Ha
authors.initials: MH
authors.lastname: Song
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Min-Ki
authors.initials: MK
authors.lastname: Lee
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Park
authors.forename: Lloyd
authors.initials: L
authors.lastname: Old
authors.forename: Sang-Yull
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D001483:Base Sequence; D001709:Biotechnology; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018899:CpG Islands; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D000077209:Decitabine; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D011401:Promoter Regions, Genetic; D012334:RNA, Neoplasm / Q000235:genetics; D013737:Testis / Q000276:immunology*; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D017931:DNA Primers; C473333:NY-SAR-35 antigen, human; D012334:RNA, Neoplasm; D000077209:Decitabine; D001374:Azacitidine