title: Control of postharvest Botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia.
abstract: A study was conducted to identify volatile organic compounds or volatiles produced by Candida intermedia strain C410 using gas chromatography-mass spectrometry, and to determine efficacy of the volatiles of C. intermedia in suppression of conidial germination and mycelial growth of Botrytis cinerea and control of Botrytis fruit rot of strawberry. Results showed that, among 49 volatiles (esters, alcohols, alkenes, alkanes, alkynes, organic acids, ketones, and aldehydes) identified from C. intermedia cultures on yeast extract peptone dextrose agar, two compounds, 1,3,5,7-cyclooctatetraene and 3-methyl-1-butanol, were the most abundant. Synthetic chemicals of 1,3,5,7-cyclooctatetraene; 3-methyl-1-butanol; 2-nonanone; pentanoic acid, 4-methyl-, ethyl ester; 3-methyl-1-butanol, acetate; acetic acid, pentyl ester; and hexanoic acid, ethyl ester were highly inhibitory to conidial germination and mycelial growth of B. cinerea. Inhibition of conidial germination and mycelial growth of B. cinerea by volatiles of C. intermedia was also observed. Meanwhile, results showed that incidence and severity of Botrytis fruit rot of strawberry was significantly (P < 0.01) reduced by exposure of the strawberry fruit to the volatiles from C. intermedia cultures or C. intermedia-infested strawberry fruit. These results suggest that the volatiles of C. intermedia C410 are promising biofumigants for control of Botrytis fruit rot of strawberry.
authors.affiliation: State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: R
authors.initials: R
authors.lastname: Huang
authors.forename: G Q
authors.initials: GQ
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: H J
authors.initials: HJ
authors.lastname: Che
authors.forename: D H
authors.initials: DH
authors.lastname: Jiang
authors.forename: H C
authors.initials: HC
authors.lastname: Huang
mesh_terms: D000935:Antifungal Agents / Q000145:classification / Q000494:pharmacology*; D001681:Biological Assay; D020171:Botrytis / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D002175:Candida / Q000737:chemistry* / Q000254:growth & development; D031985:Fragaria / Q000382:microbiology*; D005638:Fruit / Q000382:microbiology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010935:Plant Diseases / Q000382:microbiology*; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D055549:Volatile Organic Compounds / Q000145:classification / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D055549:Volatile Organic Compounds