title: Evaluation of an intraperitoneal ovarian cancer syngeneic mouse model using 18F-FDG MicroPET imaging.
abstract: OBJECTIVE
  The objective of this study was to evaluate the syngeneic immunocompetent mouse model by using the micro-positron emission tomography with 2-[fluorine-18]-fluoro-2-deoxy-d-glucose (F-FDG microPET) imaging of ovarian tumor growth.
  
  
  METHODS
  ID8 ovarian carcinoma cells derived from C57BL/6 mice were intraperitoneally injected into female C57BL/6 mice. Mice were injected with F-FDG (7.4 MBq, intravenous injection), and microPET images were obtained 40 minutes later. Micro-computed tomographic images were also obtained immediately after microPET images for anatomical reference. F-FDG microPET images were acquired at baseline and at 4, 8, 10, and 11 weeks after tumor cell injection. The maximum standardized uptake value (SUVmax) in each time point was obtained from the images and compared to follow the tumor growth.
  
  
  RESULTS
  Physiological uptake of F-FDG was intensely found in the bladder and heart and frequently in the gastrointestinal tract. Diffused uptake of F-FDG was observed in the peritoneal cavity of all tumor-bearing mice at 4 weeks, and high focal uptakes were developed in the peritoneal cavity at 8 to 11 weeks. High focal uptakes increased over time, correlating with a progressive increase in the SUVmax of F-FDG. At 11 weeks, the SUVmax value was significantly increased (1.49 ± 0.10 at 11 weeks vs 0.29 ± 0.03 at baseline, P < 0.01). Tumors in the gut and peritoneum were confirmed by anatomical and histopathological examination.
  
  
  CONCLUSIONS
  Our results demonstrate that the peritoneal tumor growth in the syngeneic ovarian cancer model can be detected by the F-FDG microPET imaging.
authors.affiliation: Department of Radiology and Radiological Sciences, The Institute of Imaging Science, Vanderbilt University Medical Center, Nashville, TN 37232, USA. hye-jeong.lee@vanderbilt.edu
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mohammed N
authors.initials: MN
authors.lastname: Tantawy
authors.forename: Ki Taek
authors.initials: KT
authors.lastname: Nam
authors.forename: Inyeong
authors.initials: I
authors.lastname: Choi
authors.forename: Todd E
authors.initials: TE
authors.lastname: Peterson
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Price
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18