title: Linoleic acid isomerase in Lactobacillus plantarum AKU1009a proved to be a multi-component enzyme system requiring oxidoreduction cofactors.
abstract: Linoleic acid isomerase in Lactobacillus plantarum was found to be a novel multi-component enzyme system widespread in membrane and soluble fractions. The isomerization reaction involved a hydration step, 10-hydroxy-12-octadecenoic acid production from linoleic acid, as part of the reaction, and the hydration reaction was catalyzed by the membrane fraction. Both membrane and soluble fractions were required for the whole isomerization reaction, i.e., conjugated linoleic acid (CLA) production from linoleic acid, and for CLA production from 10-hydroxy-12-octadecenoic acid, a reaction intermediate. The multi-component enzyme system was inhibited by o-phenanthroline, and divalent metal ions such as Ni(2+) and Co(2+) restored activity. Metal oxides such as VO(4)(3+), MoO(4)(2+), and MnO(4)(2+) enhanced activity. The multi-component enzyme systems required oxidoreduction cofactors such as NADH together with FAD or NADPH for total activity.
authors.affiliation: Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kishino
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yokozeki
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
mesh_terms: D002474:Cell-Free System / Q000187:drug effects / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D003067:Coenzymes / Q000378:metabolism*; D007535:Isomerases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology*; D019787:Linoleic Acid / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated / Q000096:biosynthesis; D008670:Metals / Q000494:pharmacology; D010084:Oxidation-Reduction
chemical_list: D002614:Chelating Agents; D003067:Coenzymes; D044243:Linoleic Acids, Conjugated; D008670:Metals; D019787:Linoleic Acid; D007535:Isomerases