title: Purification and structure elucidation of three naturally bioactive molecules from the new terrestrial Streptomyces sp. TN17 strain.
abstract: Thirty litres of fermentation broth was extracted from the newly isolated Streptomyces sp. strain TN17 and various separation and purification steps led to the isolation of three pure bioactive compounds (1-3). Compound 1: cyclo (L-Leu-L-Arg), a diketopiperazine 'DKP' derivative; 2: di-(2-ethylhexyl) phthalate, a phthalate derivative; and 3: cyclo 1-[2-(cyclopentanecarbonyl-3-phenyl-propionyl]-pyrrolidine-2-carboxylic acid (1-carbamoyl-propyl)-amide, a cyclic tetrapeptide derivative. The chemical structure of these three active compounds was established on the basis of spectroscopic studies (MS and NMR) and by comparison with data from the literature. According to our biological studies, the pure compounds (1-3) possess antibacterial and antifungal activities.
authors.affiliation: Laboratoire d'Enzymes et de Metabolites des Procaryotes (LEMP), Centre de Biotechnologie de Sfax (CBS), 3018 Sfax, Tunisie.
authors.forename: Slim
authors.initials: S
authors.lastname: Smaoui
authors.forename: Lotfi
authors.initials: L
authors.lastname: Mellouli
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lebrihi
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coppel
authors.forename: Lilia Fourati Ben
authors.initials: LF
authors.lastname: Fguira
authors.forename: Florence
authors.initials: F
authors.lastname: Mathieu
mesh_terms: D000935:Antifungal Agents; D054659:Diketopiperazines / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D013058:Mass Spectrometry; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000378:metabolism; D010795:Phthalic Acids / Q000737:chemistry* / Q000378:metabolism; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D054659:Diketopiperazines; D009842:Oligopeptides; D010456:Peptides, Cyclic; D010795:Phthalic Acids; C560131:cyclo 1-(2-(cyclopentanecarbonyl-3-phenyl-propionyl)pyrrolidine-2-carboxylic acid (1-carbamoyl-propyl)amide); C560129:cyclo(Leu-Arg)