title: Proteomic analysis of pancreatic ductal adenocarcinoma cells reveals metabolic alterations.
abstract: In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.
authors.affiliation: Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia 20110, United States. wzhou@gmu.edu
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.forename: Michela
authors.initials: M
authors.lastname: Capello
authors.forename: Claudia
authors.initials: C
authors.lastname: Fredolini
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Piemonti
authors.forename: Lance A
authors.initials: LA
authors.lastname: Liotta
authors.forename: Francesco
authors.initials: F
authors.lastname: Novelli
authors.forename: Emanuel F
authors.initials: EF
authors.lastname: Petricoin
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D021441:Carcinoma, Pancreatic Ductal / Q000737:chemistry* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000737:chemistry* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome