title: Immunogenicity of a scalable inactivated rotavirus vaccine in mice.
abstract: Inactivated rotavirus vaccine is a safe and effective potential vaccine for the prevention of rotavirus infection among children, but no approved licensed vaccine is available now. In this study, a scalable inactivated rotavirus vaccine, prepared in Vero cells cultured by microcarrier fermentation, inactivated by formalin and absorbed by Al(OH)(3) adjuvant, was vaccinated into the six weeks-old female Balb/c mice by intramuscular injection. After twice immunization at interval of three weeks, both humoral and cell-mediated immune responses were assessed by ELISA, microneutralization assay and EISPOT assay. The results indicated that the scalable inactivated rotavirus vaccines induced not only high serum IgG antibody and neutralizing antibody responses, but Th1 and Th2 cytokine-secreting cell responses in mice immunized by the inactivated rotavirus vaccines. These results suggest that the scalable inactivated rotavirus vaccine has good immunogenicity, which provided the base for the scaled development of inactivated rotavirus vaccine in the future.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, Kunming, Yunnan Provence, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Yi
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Ma
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhang
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tianhong
authors.initials: T
authors.lastname: Xie
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.forename: Maosheng
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D022243:Rotavirus Vaccines / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D022243:Rotavirus Vaccines; D015164:Vaccines, Inactivated