title: Oxidative stress parameters of L929 cells cultured on plasma-modified PDLLA scaffolds.
abstract: Oxidative stress may produce high level of reactive oxygen species (ROS) following cell exposure to endogenous and exogenous factors. Recent experiments implicate oxidative stress as playing an essential role in cytotoxicity of many materials. The aim of this study was to measure intracellular malondialdehyde (MDA), advanced oxidation protein product (AOPP) levels, and superoxide dismutase (SOD) activities of L929 fibroblasts cultured on PDLLA, polyethylene glycol (PEG), or ethylenediamine (EDA) grafted PDLLA by plasma polymerization method. Cell proliferation on these scaffolds was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The study showed that MDA, AOPP levels, and SOD activities in L929 fibroblast cells cultured on all scaffolds were significantly different compared to the control group and each other. The highest MDA (0.42 ± 0.76 nmol/mg protein), AOPP (14.99 ± 4.67 nmol/mg protein) levels, and SOD activities (7.49 ± 3.74 U/mg protein) were observed in cells cultured on non-modified scaffolds; meanwhile, the most cell proliferation was obtained in EDA-modified scaffolds (MDA 0.15 ± 0.14 nmol/mg protein, AOPP 13.12 ± 3.86 nmol/mg protein, SOD 4.82 ± 2.64 U/mg protein). According to our finding, EDA- or PEG-modified scaffolds are potentially useful as suitable biomaterials in tissue engineering.
authors.affiliation: School of Health, Aksaray University, Aksaray, Turkey. melikerol@yahoo.com
authors.forename: Melike Erol
authors.initials: ME
authors.lastname: Demirbilek
authors.forename: Murat
authors.initials: M
authors.lastname: Demirbilek
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Karahaliloğlu
authors.forename: Ebru
authors.initials: E
authors.lastname: Erdal
authors.forename: Tayfun
authors.initials: T
authors.lastname: Vural
authors.forename: Eda
authors.initials: E
authors.lastname: Yalçın
authors.forename: Necdet
authors.initials: N
authors.lastname: Sağlam
authors.forename: Emir Baki
authors.initials: EB
authors.lastname: Denkbaş
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D010949:Plasma / Q000737:chemistry; D058105:Polymerization; D013482:Superoxide Dismutase / Q000378:metabolism; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D008315:Malondialdehyde; D013482:Superoxide Dismutase