title: Biological signalling activity measurements using mass spectrometry.
abstract: MS (mass spectrometry) techniques are rapidly evolving to high levels of performance and robustness. This is allowing the application of these methods to the interrogation of signalling networks with unprecedented depth and accuracy. In the present review we discuss how MS-based multiplex quantification of kinase activities and phosphoproteomics provide complementary means to assess biological signalling activity. In addition, we discuss how a wider application of these analytical concepts to quantify kinase signalling will result in a more comprehensive understanding of normal and disease biology at the system level.
authors.affiliation: Analytical Signalling Laboratory, Centre for Cell Signalling, Institute of Cancer, Bart's and the London School of Medicine, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. p.cutillas@qmul.ac.uk
authors.forename: Pedro R
authors.initials: PR
authors.lastname: Cutillas
authors.forename: Claus
authors.initials: C
authors.lastname: Jørgensen
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D008954:Models, Biological; D010750:Phosphoproteins / Q000032:analysis*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D010770:Phosphotransferases / Q000032:analysis; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction
chemical_list: D010750:Phosphoproteins; D010770:Phosphotransferases; D010744:Phosphoric Monoester Hydrolases