title: The differentiation of the newborn nerve cells in oculomotor nuclear after oculomotor nerve injury.
abstract: Oculomotor nerve injury is a common complication of cranial trauma and craniotomy. For a long time, it has been generally considered that the oculomotor nerve is unable to regenerate and recover functionally after injury. With the development of neuroradiology, microsurgery and neurohistology, it has been reported that the injured oculomotor nerve could be repaired by operation. However, the mechanisms of neural regeneration of the injured oculomotor nerve remain obscure. Therefore, by investigating the differentiation of the newborn nerve cells in oculomotor nuclear after oculomotor nerve injury, the mechanisms of the neural regeneration of the injured oculomotor nerve was studied in the present paper. After animal model establishment, we found that the function of the injured oculomotor nerve could recover at some degree without treatment, at fourth week after the nerve injury. This result confirms that the injured oculomotor nerve per se has the potential to regenerate and repair. At the present study, by BredU stain, BrdU labeling cells were observed in oculomotor nuclear at the fourth week post-operatively. It indicated that the oculomotor nuclear per se has the ability of generating the cells, which will regenerate and differentiate after the nerve injury, without stimulation by exogenous agents. Immunofluorescence double staining was used in this study to show the differentiation of the newborn cells in oculomotor nuclear after oculomotor nerve injury. It is found that they could differentiate into neural progenitor cells, neuronal cells and neuroglial cells. It is suggested that the different differentiation of cells may play a role in the nerve regeneration procedure.
authors.affiliation: Department of Neurosurgery, Xinhua Hospital of Shanghai Jiaotong University, No. 1665 Kongjiang Road, Shanghai, 200092, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Ningxi
authors.initials: N
authors.lastname: Zhu
authors.forename: Youqiang
authors.initials: Y
authors.lastname: Meng
authors.forename: Xuhui
authors.initials: X
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.forename: Liang
authors.initials: L
authors.lastname: Wan
authors.forename: Wenchuan
authors.initials: W
authors.lastname: Zhang
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Visocchi
authors.forename: Shugan
authors.initials: S
authors.lastname: Zhu
authors.forename: Shiting
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004285:Dogs; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology*; D018613:Microscopy, Confocal; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology*; D058953:Neural Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D009802:Oculomotor Nerve / Q000166:cytology; D061220:Oculomotor Nerve Injuries