title: Apoptosis and membrane permeabilisation induced by macranthoside B on HL-60 cells.
abstract: Triterpene saponins are throught to be potential anti-tumour agents in many cell types. This study aims to evaluate the cytotoxic activity and mechanism of a triterpene saponin, macranthoside B (MB), isolated from Lonicera macranthoides Hand.-Mazz. (Caprifoliaceae). A cell viability assay showed that MB inhibited cell growth of a panel of six cancer cell lines, especially in human acute promyelocytic leukaemia HL-60 cells, with an IC50 value of 3.8 µmol. A hypodiploid cells assay and an annexin-V-FITC/PI double staining assay showed a significant increase of apoptosis in a dose-dependent manner on HL-60 cells both 24 and 48 h after MB treatment. MB-induced apoptosis was through the caspase-mediated pathway, by activation of caspase-3. Furthermore, a lactate dehydrogenase (LDH) release test suggested that an MB-cholesterol interaction led to the rearrangement of the lipid bilayer and to subsequent cell membrane impairment. Taken together, these findings demonstrate that MB may exhibit cytotoxic activity against HL-60 cells by inducing apoptosis via caspase-dependent pathways and also membrane permeabilisation.
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Mem. Sun Yat-sen, Nanjing, PR, China.
authors.forename: Fuqin
authors.initials: F
authors.lastname: Guan
authors.forename: Yu
authors.initials: Y
authors.lastname: Shan
authors.forename: Xingzeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Peng
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Xu
authors.initials: X
authors.lastname: Feng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D031239:Lonicera / Q000737:chemistry; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*
chemical_list: D012503:Saponins; C543144:macranthoside B; D009828:Oleanolic Acid; D053148:Caspase 3