title: Detection of global DNA methylation and paternally imprinted H19 gene methylation in preeclamptic placentas.
abstract: Preeclampsia (PE) is a severe hypertensive disorder associated with pregnancy; despite substantial research effort in the past several years, the etiology of PE is still unclear. The role of epigenetic factors in the etiology of PE, including DNA methylation, has been poorly characterized. In the present study, we investigated global DNA methylation as well as DNA methylation of the paternally imprinted H19 gene in preeclamptic placentas. Using 5-methylcytosine immunohistochemistry and Alu and LINE-1 repeat pyrosequencing, we found that the global DNA methylation level and the DNA (cytosine-5) methyltransferase 1 mRNA level were significantly higher in the early-onset preeclamptic placentas when compared with the normal controls. Data from methylation-sensitive high resolution melting demonstrated hypermethylation of the promoter region of the H19 gene, and results of real-time PCR showed decreased mRNA expression of H19 gene in the early-onset preeclamptic placentas as compared with the normal controls. Our results suggest that abnormal DNA methylation during placentation might be involved in the pathophysiology of PE, especially early-onset preeclampsia.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Wen-long
authors.initials: WL
authors.lastname: Gao
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Zhong-xin
authors.initials: ZX
authors.lastname: Xiao
authors.forename: Qin-ping
authors.initials: QP
authors.lastname: Liao
authors.forename: Hui-xia
authors.initials: HX
authors.lastname: Yang
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Ji
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000328:Adult; D019175:DNA Methylation; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*
chemical_list: C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated