title: Effects of ultrasound pretreatment on the enzymatic hydrolysis of soy protein isolates and on the emulsifying properties of hydrolysates.
abstract: Soy protein isolate (SPI) was modified by ultrasound pretreatment (200 W, 400 W, 600 W) and controlled papain hydrolysis, and the emulsifying properties of SPIH (SPI hydrolysates) and USPIH (ultrasound pretreated SPIH) were investigated. Analysis of mean droplet sizes and creaming indices of emulsions formed by SPIH and USPIH showed that some USPIH had markedly improved emulsifying capability and emulsion stabilization against creaming during quiescent storage. Compared with control SPI and SPIH-0.58% degree of hydrolysis (DH), USPIH-400W-1.25% (USPIH pretreated under 400W sonication and hydrolyzed to 1.25% DH) was capable of forming a stable fine emulsion (d43=1.79 μm) at a lower concentration (3.0% w/v). A variety of physicochemical and interfacial properties of USPIH-400W products have been investigated in relation to DH and emulsifying properties. SDS-PAGE showed that ultrasound pretreatment could significantly improve the accessibility of some subunits (α-7S and A-11S) in soy proteins to papain hydrolysis, resulting in changes in DH, protein solubility (PS), surface hydrophobicity (H0), and secondary structure for USPIH-400W. Compared with control SPI and SPIH-0.58%, USPIH-400W-1.25% had a higher protein adsorption fraction (Fads) and a lower saturation surface load (Γsat), which is mainly due to its higher PS and random coil content, and may explain its markedly improved emulsifying capability. This study demonstrated that combined ultrasound pretreatment and controlled enzymatic hydrolysis could be an effective method for the functionality modification of globular proteins.
authors.affiliation: College of Light Industry and Food Science, South China University of Technology, 381 Wushan Road, Guangzhou, China, 510641.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Jianshe
authors.initials: J
authors.lastname: Chen
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000327:Adsorption; D004655:Emulsions / Q000737:chemistry; D005524:Food Technology / Q000379:methods*; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D010206:Papain / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*; D012995:Solubility; D030262:Soybean Proteins / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D004655:Emulsions; D011492:Protein Hydrolysates; D030262:Soybean Proteins; D010206:Papain