title: Immunogenicity and protection against Haemophilus parasuis infection after vaccination with recombinant virulence associated trimeric autotransporters (VtaA).
abstract: Haemophilus parasuis is the etiological agent of Glässer's disease in swine, characterized by fibrinous polyserositis, polyarthritis and meningitis. The lack of a vaccine against a broad spectrum of strains has limited the control of the disease. Recently, virulence associated trimeric autotransporters (VtaA) were described as antigenic proteins of H. parasuis. In this study 6 VtaA were produced as recombinant proteins and used to immunize snatch-farrowed, colostrum-deprived piglets. Immunized animals developed specific systemic and mucosal antibodies. The protective capacity of the anti-VtaA antibodies was evaluated by the inoculation of 3 × 10(8) or 6 × 10(6) colony forming units (CFU) of the highly virulent strain Nagasaki. Vaccinated animals had a delayed course of disease and 33 or 57%, respectively, of the animals survived the lethal challenge. The partial protection achieved with the recombinant VtaA supports their potential as candidates to be included in future vaccine formulations against H. parasuis.
authors.affiliation: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain. alex.olvera@cresa.uab.cat
authors.forename: Alex
authors.initials: A
authors.lastname: Olvera
authors.forename: Sonia
authors.initials: S
authors.lastname: Pina
authors.forename: Marta
authors.initials: M
authors.lastname: Pérez-Simó
authors.forename: Virginia
authors.initials: V
authors.lastname: Aragón
authors.forename: Joaquim
authors.initials: J
authors.lastname: Segalés
authors.forename: Albert
authors.initials: A
authors.lastname: Bensaid
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D006192:Haemophilus Infections / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000276:immunology*; D018928:Immunity, Mucosal; D026901:Membrane Transport Proteins / Q000276:immunology*; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D026901:Membrane Transport Proteins; D014614:Vaccines, Synthetic; D037521:Virulence Factors