title: Genetic diversity of picoeukaryotes in eight lakes differing in trophic status.
abstract: The genetic diversity of picoeukaryotes (0.2-5.0 µm) was investigated in 8 lakes differing in trophic status in Nanjing, China. Denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 18S rRNA genes were applied to analyze the picoeukaryotic communities. DGGE analysis showed that among the 8 lakes, the diversity of picoeukaryotes was greatest in the mesotrophic Lake Nan (24 bands) and least in the oligotrophic Lake Qian (12 bands). Cluster analysis of DGGE profiles revealed that the 8 lakes were grouped into 2 distinct clusters. Cluster 1 contained lakes Mochou, Zixia, Huashen, Nan, Pipa, and Qian, while cluster 2 contained lakes Xuanwu and Baijia. Clone libraries were constructed from the mesotrophic Lake Xuanwu and the oligotrophic Lake Zixia, and the 2 libraries were compared using the program LIBSHUFF. This analysis indicated that the picoeukaryotic community composition differed significantly between the 2 lakes (p = 0.001). A total of 25 operational taxonomic units were detected; 18 (62 clones) were related to known eukaryotic groups, while 7 (30 clones) were not affiliated with any known eukaryotic group. Alveolates and stramenopiles were the dominant groups in Lake Xuanwu, while alveolates and chlorophyta predominated in Lake Zixia. Multivariate statistical analysis indicated that the differences in the picoeukaryotic community composition of the 8 lakes might be related to trophic status and top-down regulation by metazooplankton.
authors.affiliation: State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Science, Nanjing 210008, China.
authors.forename: Biying
authors.initials: B
authors.lastname: Zhao
authors.forename: Meijun
authors.initials: M
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Yang
authors.forename: Feizhou
authors.initials: F
authors.lastname: Chen
mesh_terms: D056893:Alveolata / Q000145:classification / Q000235:genetics*; D002681:China; D000460:Chlorophyta / Q000145:classification / Q000235:genetics*; D058645:Denaturing Gradient Gel Electrophoresis; D005618:Fresh Water / Q000382:microbiology; D015723:Gene Library; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D058009:Stramenopiles / Q000145:classification / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D012337:RNA, Ribosomal, 18S