title: Development of a dispersive liquid-liquid microextraction method for spectrophotometric determination of barbituric acid in pharmaceutical formulation and biological samples.
abstract: In this paper, a novel and simple method for the determination of trace amounts of barbituric acid in water and biological samples was developed by using dispersive liquid-liquid microextraction (DLLME) techniques combined with spectrophotometric analysis. The procedure is based on color reaction of barbituric acid with p-dimethylaminobenzaldehyde and extraction of the color product using the DLLME technique. Some important parameters such as reaction conditions and the type and volume of extraction and dispersive solvents as well as the extraction time were investigated and optimized in detail. Under the optimum conditions, the calibration graphs were linear over the range of 5.0 to 200 ng ml(-1) with limit of detection of 2.0 ng ml(-1). Relative standard deviation for five replicate determinations of barbituric acid at 50 ng ml(-1) concentration level was calculated to be 1.64%. Average recoveries for spiked samples were determined to be between 94% and 105%. The proposed method was applied for the determination of barbituric acid in pharmaceutical formulation and biological samples.
authors.affiliation: Department of Chemistry, Faculty of Materials, Malek Ashtar University of Technology, P.O. Box 15875-1774, Tehran, Iran. zarei1349@gmail.com
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Zarei
authors.forename: Forouzan
authors.initials: F
authors.lastname: Gholamian
mesh_terms: D001463:Barbiturates / Q000097:blood* / Q000652:urine*; D001547:Benzaldehydes / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000652:urine*; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
chemical_list: D001463:Barbiturates; D001547:Benzaldehydes; D004396:Coloring Agents; D004364:Pharmaceutical Preparations; D012997:Solvents; D006851:Hydrochloric Acid; C510241:p-dimethylaminobenzaldehyde; C032232:barbituric acid