title: Evaluation of a DNA microarray (Check-MDR CT102) for rapid detection of TEM, SHV, and CTX-M extended-spectrum β-lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapenemases.
abstract: The Check-MDR CT102 microarray, aimed at identifying bacteria producing extended-spectrum β-lactamase (ESBL) (SHV, TEM, and CTX-M) and carbapenemase (KPC, OXA-48, VIM, IMP, and NDM-1), was evaluated on a total of 144 Gram-negative strains expressing various β-lactamases. The sensitivity and specificity were 100% for most tested genes, suggesting that this assay allows accurate identification of common ESBL and carbapenemase producers from bacterial cultures.
authors.affiliation: Service de Bactériologie-Virologie, INSERM U914, Hôpital de Bicêtre, 94275 Le Kremlin-Bicêtre, France. thierry.naas@bct.ap-hop-paris.fr
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
authors.forename: Gaelle
authors.initials: G
authors.lastname: Cuzon
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000145:classification / Q000235:genetics*
chemical_list: D001618:beta-Lactamases