title: Oligonucleotide recombination: a hidden treasure.
abstract: In Swingle et al. we demonstrate that it is possible to use recombineering to direct a variety of changes in wild-type bacterial cells without the addition of phage-encoded proteins. This discovery is potentially applicable to biological engineering in a wide variety of bacterial species. Here we describe key features of oligo recombination as it is currently understood, and propose strategies for expanding the utility of oligo recombination for bioengineering.
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, Ithaca, NY, USA. Swingle@ars.usda.gov
authors.forename: Bryan
authors.initials: B
authors.lastname: Swingle
authors.forename: Eric
authors.initials: E
authors.lastname: Markel
authors.forename: Samuel
authors.initials: S
authors.lastname: Cartinhour
mesh_terms: D057005:Bioengineering / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D009841:Oligonucleotides / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D009841:Oligonucleotides