title: Are chlamydial lipopolysaccharide-directed antibodies in seminal plasma or serum clinically significant during investigation of male infertility?
abstract: OBJECTIVE
  To analyze the clinical relevance of chlamydial serology in seminal plasma (SP)/serum for male fertility.
  
  
  METHODS
  We determined the IgG, IgA, and IgM class antibodies to Chlamydia trachomatis with a lipopolysaccharide-directed standard enzyme-linked immunosorbent assay in the serum of 173 asymptomatic subfertile couples and in the same-day SP in a prospective study. The semen quality evaluation was done using aliquots of the same ejaculate (eg, sperm analysis, leukocytes, bacterial cultures, sperm functional capacity). The medical history, clinical examination, postcoital testing, and determination of subsequent fertility were also done.
  
  
  RESULTS
  The antichlamydial antibodies of the 3 classes were significantly interrelated, and SP Chlamydia IgA antibodies were significantly associated with the findings in the serum. The evidence for previous or persistent chlamydial infection was significantly more frequent in the female partners of men with Chlamydia antibodies in the serum and/or Chlamydia IgA antibodies in the SP. The chlamydial serology results in the SP/serum were not associated with the sperm quality, leukocytes, semen culture findings, or sperm functional capacity.
  
  
  CONCLUSIONS
  The chlamydial serology results (ie, IgG, IgA, and IgM antibodies in SP/serum) determined using a lipopolysaccharide-directed enzyme-linked immunosorbent assay were not indicative of reduced sperm function or subsequent fertilizing capacity.
authors.affiliation: Department of Gynecological Endocrinology and Reproductive Medicine, Women's Hospital, University of Heidelberg, Heidelberg, Germany. Waltraud_Eggert-Kruse@med.uni-heidelberg.de
authors.forename: Waltraud
authors.initials: W
authors.lastname: Eggert-Kruse
authors.forename: Miriam
authors.initials: M
authors.lastname: Weltin
authors.forename: Thomas
authors.initials: T
authors.lastname: Strowitzki
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D002689:Chlamydia / Q000276:immunology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007136:Immunoglobulins / Q000032:analysis* / Q000097:blood; D007248:Infertility, Male / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012661:Semen / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; C042866:antilipopolysaccharide antibodies