title: Application of artificial gel antibodies for investigating molecular polymorphisms of human pituitary growth hormone.
abstract: Artificial gel antibodies were used to investigate human growth hormone (GH) activity in preparations purified from human pituitary glands. A partially purified fraction containing differently sized structural variants of GH was processed to yield monomeric and dimeric forms suitable for synthesizing artificial polyacrylamide gel antibodies. These two types of GH antibodies were used for investigating GH activity in experiments using HPLC gel-permeation and ion-exchange chromatography. In the size-exclusion experiments, both hormone fractions eluted as homogeneous peaks, whereas the ion exchanger resolved the hormones into several active components. The GH monomer antibodies exhibited a much higher affinity for monomeric GH than for dimeric GH, and the GH dimer antibodies exhibited a much higher affinity for dimeric GH than for monomeric GH. It was concluded that these two sets of antibodies might be useful for discriminating between dimeric and monomeric GH in clinical samples.
authors.affiliation: Department of Pharmaceutical Biosciences, Uppsala University, Uppsala, Sweden.
authors.forename: Nasim
authors.initials: N
authors.lastname: Ghasemzadeh
authors.forename: Uwe L
authors.initials: UL
authors.lastname: Rossbach
authors.forename: Britt-Marie
authors.initials: BM
authors.lastname: Johansson
authors.forename: Fred
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D019281:Dimerization; D004586:Electrophoresis; D005782:Gels / Q000138:chemical synthesis / Q000378:metabolism*; D013006:Growth Hormone / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000378:metabolism*; D018716:Molecular Mimicry; D010902:Pituitary Gland / Q000737:chemistry; D011110:Polymorphism, Genetic; D011485:Protein Binding; D020033:Protein Isoforms / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D000099166:Polyacrylamides
chemical_list: D020106:Acrylamide; D000180:Acrylic Resins; D000906:Antibodies; D005782:Gels; D013006:Growth Hormone; D007202:Indicators and Reagents; D020033:Protein Isoforms; D014357:Trypsin; D000099166:Polyacrylamides