title: Palladium(II)-mediated assembly of biotinylated ion channels.
abstract: Simple synthetic methodology has been used to create biotinylated pyridyl cholate lipids that can undergo multiple self-assembly events when inserted into phospholipid vesicles; Pd(II) links cholates into transmembrane lipids, while avidin laterally clusters these complexes together and concomitantly assembles the vesicles into aggregates. The transmembrane assembly of cholates by Pd(II) "opened" the ion channels, whereas avidin addition produced vesicle aggregates, giving a system that mimicked both transmembrane transport and cellular adhesion. Complexation of these Pd(II)-linked cholates by avidin gave a measurable decrease in ion flow, suggesting some channels became blocked or were prevented from adopting the optimum geometry for ion conduction. This reflects the importance of spatially appropriate preorganisation when generating active supramolecular assemblies.
authors.affiliation: Manchester Interdisciplinary Biocentre (MIB) and School of Chemistry, University of Manchester, 131 Princess St, Manchester, M1 7DN, UK.
authors.forename: Craig P
authors.initials: CP
authors.lastname: Wilson
authors.forename: Cécile
authors.initials: C
authors.lastname: Boglio
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.forename: Scott L
authors.initials: SL
authors.lastname: Cockroft
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Webb
mesh_terms: D019921:Biotinylation; D020355:Cholates / Q000737:chemistry*; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D015394:Molecular Structure; D010165:Palladium / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D020355:Cholates; D007473:Ion Channels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010743:Phospholipids; D010165:Palladium