title: HCMV gB genotype and its association with cytokine levels in hematopoietic stem cell transplantation.
abstract: BACKGROUND
  Glycoprotein B (gB) has been implicated in determining the pathogenicity and clinical outcomes of human cytomegalovirus (HCMV) disease.
  
  
  OBJECTIVE
  The purpose of this study was to assess the prevalence of gB genotypes in allogeneic hematopoietic stem cell transplantation (allo-HSCT) and the relationship between it and cytokine levels in saliva and blood samples. The impact of these parameters on patients' survival was also investigated.
  
  
  METHODS
  Samples were obtained from 63 patients receiving an allo-HSCT. HCMV gB genotyping was carried out by multiplex nested PCR. The cytokine levels were assessed using ELISA assay.
  
  
  RESULTS
  A single or mixed genotype infection was detected in the saliva and blood of 36/63 and 52/63 subjects, respectively. Patients with gB2 in their saliva showed lower IL-10 levels in comparison with patients without gB2. Reduced blood levels of IFN-γ and IL-1β were also found in recipients with the HCMV gB4 genotype compared with patients without it. Decreased IL-1β and increased IL-10 blood levels were associated with lower survival. However, HCMV gB genotypes have no impact on patient outcome.
  
  
  CONCLUSIONS
  Decreased IL-1β and increased IL-10 levels in the blood are associated with lower survival. HCMV genotypes are associated with different cytokine levels in saliva and blood.
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Correia-Silva
authors.forename: R G
authors.initials: RG
authors.lastname: Resende
authors.forename: T C
authors.initials: TC
authors.lastname: Arão
authors.forename: M H N G
authors.initials: MH
authors.lastname: Abreu
authors.forename: M M
authors.initials: MM
authors.lastname: Teixeira
authors.forename: H
authors.initials: H
authors.lastname: Bittencourt
authors.forename: T A
authors.initials: TA
authors.lastname: Silva
authors.forename: R S
authors.initials: RS
authors.lastname: Gomez
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis* / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016867:Immunocompromised Host; D007371:Interferon-gamma / Q000032:analysis / Q000097:blood; D016753:Interleukin-10 / Q000032:analysis / Q000097:blood; D053583:Interleukin-1beta / Q000032:analysis / Q000097:blood; D015850:Interleukin-6 / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D009894:Opportunistic Infections / Q000821:virology; D012463:Saliva / Q000737:chemistry / Q000276:immunology; D015996:Survival Rate; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D016753:Interleukin-10; D007371:Interferon-gamma