title: High-density microcarrier cell cultures for influenza virus production.
abstract: Influenza virus A/PR/8/34 virus propagation in adherent Madin-Darby canine kidney cells in high-density microcarrier cultures is described. To improve virus yields, perfusion and repeated fed-batch modes were applied using cell-specific feed rates. Cell densities up to 1.1 × 10(7) cells/mL were achieved. Cell-specific virus yields in high-density cultures were at similar levels compared with standard, low-density cultivations. In the average 2,400 and 3,300 virions per cell were obtained for two variants of the virus strain A/PR/8/34, PR8-National Institute for Biological Standards and Control (NIBSC) and PR8-Robert Koch Institute, respectively. Maximum virus titer (HA activity = 1,778 HAU/100 μL) for virus variant PR8-NIBSC was obtained for a cultivation infected before maximum cell concentration was reached.
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, 39106 Magdeburg, Germany. bock@mpi-magdeburg.mpg.de
authors.forename: A
authors.initials: A
authors.lastname: Bock
authors.forename: J
authors.initials: J
authors.lastname: Schulze-Horsel
authors.forename: J
authors.initials: J
authors.lastname: Schwarzer
authors.forename: E
authors.initials: E
authors.lastname: Rapp
authors.forename: Y
authors.initials: Y
authors.lastname: Genzel
authors.forename: U
authors.initials: U
authors.lastname: Reichl
mesh_terms: D000818:Animals; D019149:Bioreactors; D002460:Cell Line; D003470:Culture Media; D004285:Dogs; D009980:Influenza A virus / Q000502:physiology*; D014779:Virus Replication
chemical_list: D003470:Culture Media