title: Biocatalytic synthesis and antitumor activities of novel silybin acylated derivatives with dicarboxylic acids.
abstract: Novel silybin acylated derivatives with dicarboxylic acids were prepared in various organic solvents using immobilized Candida antarctica lipase B (Novozym 435(®)). The reaction parameters affecting the silybin conversion, such as the nature of the organic solvent and the acyl donor used were investigated. The antiproliferative effects of silybin monoesters, and their ability to modulate the secretion of vascular endothelial growth factor (VEGF) were estimated using K562 human lymphoblastoma cells and compared to the parental compound. The synthesized esters retained the biological function of silybin and in some cases were more effective, indicating that target biotransformation may generate novel compounds with improved antitumor and antiangiogenic activities.
authors.affiliation: Laboratory of Biotechnology, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, 15700 Athens, Greece.
authors.forename: Eleni
authors.initials: E
authors.lastname: Theodosiou
authors.forename: Heleni
authors.initials: H
authors.lastname: Loutrari
authors.forename: Haralambos
authors.initials: H
authors.lastname: Stamatis
authors.forename: Charis
authors.initials: C
authors.lastname: Roussos
authors.forename: Fragiskos N
authors.initials: FN
authors.lastname: Kolisis
mesh_terms: D000107:Acetylation; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects*; D003998:Dicarboxylic Acids / Q000737:chemistry*; D005656:Fungal Proteins; D006801:Humans; D020014:K562 Cells; D008049:Lipase / Q000737:chemistry*; D000077385:Silybin; D012838:Silymarin / Q000138:chemical synthesis* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D003998:Dicarboxylic Acids; D005656:Fungal Proteins; D012838:Silymarin; D000077385:Silybin; D008049:Lipase; C431349:lipase B, Candida antarctica