title: Large volume leukapheresis: Efficacy and safety of processing patient's total blood volume six times.
abstract: Large-volume leukapheresis (LVL) differs from standard leukapheresis by increased blood flow and an altered anticoagulation regimen. An open issue is to what degree a further increase in processed blood volume is reasonable in terms of higher yields and safety. In 30 LVL performed in patients with hematologic malignancies, 6 total blood volumes were processed. LVL resulted in a higher CD34+ cell yield without a change in graft quality. Although a marked platelet decrease can be expected, LVL is safe and can be recommended as the standard procedure for patients who mobilize low numbers of CD34+ cells and when high number of CD34+ cells are required.
authors.affiliation: Department of Transfusion Medicine and Cellular Therapy, University Hospital Centre Zagreb, Kispaticeva 12, Croatia. ibojanic@kbc-zagreb.hr
authors.forename: Ines
authors.initials: I
authors.lastname: Bojanic
authors.forename: Klara
authors.initials: K
authors.lastname: Dubravcic
authors.forename: Drago
authors.initials: D
authors.lastname: Batinic
authors.forename: Branka Golubic
authors.initials: BG
authors.lastname: Cepulic
authors.forename: Sanja
authors.initials: S
authors.lastname: Mazic
authors.forename: Darko
authors.initials: D
authors.lastname: Hren
authors.forename: Damir
authors.initials: D
authors.lastname: Nemet
authors.forename: Boris
authors.initials: B
authors.lastname: Labar
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000096:biosynthesis; D001792:Blood Platelets / Q000378:metabolism; D004573:Electrolytes; D005260:Female; D019337:Hematologic Neoplasms / Q000628:therapy*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006801:Humans; D007700:Kinetics; D007937:Leukapheresis / Q000379:methods*; D007962:Leukocytes / Q000166:cytology; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D012449:Safety
chemical_list: D018952:Antigens, CD34; D004573:Electrolytes