title: Heterologous expression and characterization of the sterol 14α-demethylase CYP51F1 from Candida albicans.
abstract: Lanosterol 14α-demethylase (CYP51F1) from Candida albicans is known to be an essential enzyme in fungal sterol biosynthesis. Wild-type CYP51F1 and several of its mutants were heterologously expressed in Escherichia coli, purified, and characterized. It exhibited a typical reduced CO-difference spectrum with a maximum at 446 nm. Reconstitution of CYP51F1 with NADPH-P450 reductase gave a system that successfully converted lanosterol to its demethylated product. Titration of the purified enzyme with lanosterol produced a typical type I spectral change with K(d)=6.7 μM. The azole antifungal agents econazole, fluconazole, ketoconazole, and itraconazole bound tightly to CYP51F1 with K(d) values between 0.06 and 0.42 μM. The CYP51F1 mutations F105L, D116E, Y132H, and R467K frequently identified in clinical isolates were examined to determine their effect on azole drug binding affinity. The azole K(d) values of the purified F105L, D116E, and R467K mutants were little altered. A homology model of C. albicans CYP51F1 suggested that Tyr132 in the BC loop is located close to the heme in the active site, providing a rationale for the modified heme environment caused by the Y132H substitution. Taken together, functional expression and characterization of CYP51F1 provide a starting basis for the design of agents effective against C. albicans infections.
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hyoung-Goo
authors.initials: HG
authors.lastname: Park
authors.forename: Im-Soon
authors.initials: IS
authors.lastname: Lee
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Chun
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Yun
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Johnston
authors.forename: Paul R Ortiz de
authors.initials: PR
authors.lastname: Montellano
authors.forename: Donghak
authors.initials: D
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D007810:Lanosterol / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016415:Sequence Alignment; D013053:Spectrophotometry; D058886:Sterol 14-Demethylase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D007810:Lanosterol; D058886:Sterol 14-Demethylase