title: Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.
abstract: After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain connected by an intercellular bridge, which subsequently splits by abscission. Here, we examined intermediate stages of abscission in human cells by using live imaging, three-dimensional structured illumination microscopy, and electron tomography. We identified helices of 17-nanometer-diameter filaments, which narrowed the cortex of the intercellular bridge to a single stalk. The endosomal sorting complex required for transport (ESCRT)-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments. Simultaneous spastin-mediated removal of underlying microtubules enabled full constriction at the abscission site. The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures.
authors.affiliation: Institute of Biochemistry, Department of Biology, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, Zurich, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Guizetti
authors.forename: Lothar
authors.initials: L
authors.lastname: Schermelleh
authors.forename: Jana
authors.initials: J
authors.lastname: Mäntler
authors.forename: Sandra
authors.initials: S
authors.lastname: Maar
authors.forename: Ina
authors.initials: I
authors.lastname: Poser
authors.forename: Heinrich
authors.initials: H
authors.lastname: Leonhardt
authors.forename: Thomas
authors.initials: T
authors.lastname: Müller-Reichert
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Gerlich
mesh_terms: D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D055032:Electron Microscope Tomography; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000378:metabolism; D011487:Protein Conformation; D055503:Protein Multimerization; D034622:RNA Interference; D000074181:Spastin
chemical_list: D000199:Actins; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; C505378:Cep55 protein, human; D056827:Endosomal Sorting Complexes Required for Transport; D009687:Nuclear Proteins; C117020:PDCD6IP protein, human; D000251:Adenosine Triphosphatases; D000074181:Spastin; C400283:SPAST protein, human