title: Synthesis of novel tricalcium phosphate-bioactive glass composite and functionalization with rhBMP-2.
abstract: A functionalization is required for calcium phosphate-based bone substitute materials to achieve an entire bone remodeling. In this study it was hypothesized that a tailored composite of tricalcium phosphate and a bioactive glass can be loaded sufficiently with rhBMP-2 for functionalization. A composite of 40 wt% tricalcium phosphate and 60 wt% bioactive glass resulted in two crystalline phases, wollastonite and rhenanite after sintering. SEM analysis of the composite's surface revealed a spongious bone-like morphology after treatment with different acids. RhBMP-2 was immobilized non-covalently by treating with chrome sulfuric acid (CSA) and 3-aminopropyltriethoxysilane (APS) and covalently by treating with CSA/APS, and additionally with 1,1'-carbonyldiimidazole. It was proved that samples containing non-covalently immobilized rhBMP-2 on the surface exhibit significant biological activity in contrast to the samples with covalently bound protein on the surface. We conclude that a tailored composite of tricalcium phosphate and bioactive glass can be loaded sufficiently with BMP-2.
authors.affiliation: Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.
authors.forename: Karolina
authors.initials: K
authors.lastname: Schickle
authors.forename: Kristin
authors.initials: K
authors.lastname: Zurlinden
authors.forename: Christian
authors.initials: C
authors.lastname: Bergmann
authors.forename: Markus
authors.initials: M
authors.lastname: Lindner
authors.forename: Armin
authors.initials: A
authors.lastname: Kirsten
authors.forename: Markus
authors.initials: M
authors.lastname: Laub
authors.forename: Rainer
authors.initials: R
authors.lastname: Telle
authors.forename: Herbert
authors.initials: H
authors.lastname: Jennissen
authors.forename: Horst
authors.initials: H
authors.lastname: Fischer
mesh_terms: D016475:3T3 Cells; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry; D016723:Bone Remodeling; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011437:Propylamines; D011994:Recombinant Proteins / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D007093:Imidazoles; D011437:Propylamines; D011994:Recombinant Proteins; D012821:Silanes; D013464:Sulfuric Acids; C006900:N,N-carbonyldiimidazole; C018392:tricalcium phosphate; C477625:amino-propyl-triethoxysilane; C033158:sulfuric acid