title: [The construction and expression of phage display library of anti human lung adenocarcinoma monoclonal antibody 5F-11].
abstract: BACKGROUND
  To construct and express a phage display library of anti human lung cancer monoclonal antibody 5F-11.
  
  
  METHODS
  Immunoglobulin variable regions (VH,VL) were amplified from 5F-11 hybridrom by RT-PCR. ScFv genes consisting of VH DNA and VL DNA joined together by a linker DNA were cloned into a phage vector pCANTAB5E. After 4 rounds of screening with lung adenocarcinoma cell line A2 as antigen, an enriched secondary phage display library was obtained.
  
  
  RESULTS
  A recombinant phage display library with total of 8×10⁷ pfu/ml was established. Randomized clones from unselected library digested with BstNⅠ showed different patterns, however, those from selected library showed that phages with special pattern were enriched. Twenty-three out of 30 clones were found to respond strongly to A2 cell lines.
  
  
  CONCLUSIONS
  The ScFv of anti-lung adenocarcinoma monoclonal antibody 5F-11 can be successfully produced, which may be useful to widen the application of the antibody.
authors.affiliation: Branch Laboratory of Lung Cancer, Beijing Laboratory of Cancer Molecule, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing 101149, P.R.China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Yue
authors.forename: Baitang
authors.initials: B
authors.lastname: Lai
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiuping
authors.initials: X
authors.lastname: Zhan
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.forename: Xuehui
authors.initials: X
authors.lastname: Yang