title: Fluorescent and lanthanide labeling for ligand screens, assays, and imaging.
abstract: The use of fluorescent (or luminescent) and metal contrast agents in high-throughput screens, in vitro assays, and molecular imaging procedures has rapidly expanded in recent years. Here we describe the development and utility of high-affinity ligands for cancer theranostics and other in vitro screening -studies. In this context, we also illustrate the syntheses and use of heteromultivalent ligands as targeted imaging agents.
authors.affiliation: Department of Chemistry, University of Arizona, Tucson, AZ, USA.
authors.forename: Jatinder S
authors.initials: JS
authors.lastname: Josan
authors.forename: Channa R
authors.initials: CR
authors.lastname: De Silva
authors.forename: Byunghee
authors.initials: B
authors.lastname: Yoo
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Lynch
authors.forename: Mark D
authors.initials: MD
authors.lastname: Pagel
authors.forename: Josef
authors.initials: J
authors.lastname: Vagner
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Hruby
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006573:Heterocyclic Compounds, 1-Ring / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D028581:Lanthanoid Series Elements / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000188:drug therapy; D004369:Pentetic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D028581:Lanthanoid Series Elements; D008024:Ligands; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D004369:Pentetic Acid