title: Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA.
abstract: RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
authors.affiliation: Biomedicine research and development center of Jinan University, Guangdong, Guangzhou 510632, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ren
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Qiao-li
authors.initials: QL
authors.lastname: Wang
authors.forename: Yang-fei
authors.initials: YF
authors.lastname: Xiang
authors.forename: Yun-xia
authors.initials: YX
authors.lastname: Cui
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Ren-bin
authors.initials: RB
authors.lastname: Qi
authors.forename: Da-xiang
authors.initials: DX
authors.lastname: Lu
authors.forename: Shu-min
authors.initials: SM
authors.lastname: Zhang
authors.forename: Pei-zhuo
authors.initials: PZ
authors.lastname: Zhang
mesh_terms: D018259:Herpesvirus 1, Human / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012264:Ribonucleotide Reductases / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014764:Viral Proteins; D012264:Ribonucleotide Reductases