title: Development of activity-based probes for cathepsin X.
abstract: Cathepsin X is a lysosomal cysteine protease that functions as a carboxypeptidase with broad substrate specificity. Cathepsin X was discovered only recently, and its physiological roles are still not well understood. A number of studies suggest that cathepsin X may be involved in a variety of biological processes, including cancer, aging and degenerative conditions of the brain, inflammation, and cellular communication. Here we present the synthesis and characterization of several activity-based probes (ABPs) that target active cathepsin X. These ABPs were used to label cathepsin X in complex lysates, whole cells, and in vivo. Furthermore, we have developed a method for selectively labeling and visualizing active cathepsin X in vitro and in vivo. Overall, the probes developed in this study are valuable tools for the study of cathepsin X function.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, 300 Pasteur Dr., Stanford, CA 94305-5324, United States.
authors.forename: Margot G
authors.initials: MG
authors.lastname: Paulick
authors.forename: Matthew
authors.initials: M
authors.lastname: Bogyo
mesh_terms: D000818:Animals; D056657:Cathepsin K / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D051381:Rats; D013194:Staining and Labeling; D013237:Stereoisomerism; D013379:Substrate Specificity; D014018:Tissue Distribution
chemical_list: D005456:Fluorescent Dyes; D056657:Cathepsin K