title: Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus α-galactosidase.
abstract: Using Response Surface Methodology, carbon and nitrogen sources and agitation speed for cultivation of Aspergillus sojae expressing the α-galactosidase gene, aglB of Aspergillus fumigatus IMI 385708 were optimized. Compared to cultivation in modified YpSs medium, cultivation in 250-mL Erlenmeyer flasks agitated at 276 rpm and containing 100 mL of optimized medium consisting of 10.5% molasses (w/v) and 1.3% NH(4)NO(3) (w/v), 0.1% K(2)HPO(4), and 0.005% MgSO(4)·7H(2)O achieved a 4-fold increase in α-galactosidase production (10.4 U/mL). These results suggest the feasibility of industrial large scale production of an α-galactosidase known to be valuable in galactomannan modification.
authors.affiliation: Department of Biotechnology, Middle East Technical University, 06531 Ankara, Turkey. sumeyra@metu.edu.tr
authors.forename: Sumeyra
authors.initials: S
authors.lastname: Gurkok
authors.forename: Deniz
authors.initials: D
authors.lastname: Cekmecelioglu
authors.forename: Zumrut B
authors.initials: ZB
authors.lastname: Ogel
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D007218:Industrial Microbiology / Q000379:methods*; D013045:Species Specificity; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D003470:Culture Media; D000519:alpha-Galactosidase