title: In vitro antiinflammatory activity of a new sesquiterpene lactone isolated from Siegesbeckia glabrescens.
abstract: A new sesquiterpene lactone was isolated from Siegesbeckia glabrescens as an inhibitor of nitric oxide and prostaglandin E2 synthesis in the LPS-activated RAW264.7 macrophage cell line. It dose-dependently decreased the protein and mRNA levels of inducible nitric oxide synthase and cyclooxygenase-2. Reporter gene assay revealed that the compound attenuated the LPS-induced DNA transcriptional activity of nuclear factor-kappa B (NF-κB). In addition, it inhibited the nuclear translocation of the p65 subunit of NF-κB by blocking the inhibitory kappa B-α degradation. This compound from Siegesbeckia glabrescens might be beneficial for the treatment of inflammation-related diseases.
authors.affiliation: College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-Gil, Yongsan-Gu, Seoul, 140-742, Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Jinseong
authors.initials: J
authors.lastname: Hyeon
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D007783:Lactones; D010936:Plant Extracts; C498144:Rela protein, mouse; D012717:Sesquiterpenes; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone