title: Dicyanostilbene-derived two-photon fluorescence probe for free zinc ions in live cells and tissues with a large two-photon action cross section.
abstract: A novel two-photon fluorescence probe for Zn(2+) derived from dicyanostilbene as a two-photon fluorophore and 4-(pyridine-2-ylmethyl)piperazine as a novel Zn(2+) ligand was developed. The probe shows a 72.5-fold fluorescence enhancement in response to Zn(2+), a large two-photon action cross-section (580 GM), a noncytotoxic effect, and pH insensitivity in the biologically relevant range, and its dissociation constant (K(d)(TP)) is 0.52 ± 0.01 μM. The probe can selectively detect free Zn(2+) ions in live cells for 1500 s or so and in living tissues at a depth of 80-150 μm without interference from other metal ions and the membrane-bound probes.
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China. huangchibao@163.com
authors.forename: Chibao
authors.initials: C
authors.lastname: Huang
authors.forename: Junle
authors.initials: J
authors.lastname: Qu
authors.forename: Jing
authors.initials: J
authors.lastname: Qi
authors.forename: Meng
authors.initials: M
authors.lastname: Yan
authors.forename: Gaixia
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D008024:Ligands; D051379:Mice; D015394:Molecular Structure; D017785:Photons; D013267:Stilbenes / Q000737:chemistry*; D015032:Zinc / Q000032:analysis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D013267:Stilbenes; D015032:Zinc