title: Effects of heating coagulation of middle meningeal artery on plasma CGRP level and c-fos expression in migraine rat triggered by nitroglycerin.
abstract: Current theory or hypothesis relevant to migraine indicates that trigeminovascular system plays a pivotal role in the pathophysiology of migraine. Particularly, release of neuropeptide and induction of c-fos like immunoreactivity (c-fos LI) within trigeminal nucleus caudalis neurons are regarded as activation markers of trigeminovascular system. In the present study, we set up a rat model for migraine triggered by nitroglycerin (NTG) and coagulated the middle meningeal artery by heating. Using this model, we determined the plasma calcitonin gene-related peptide (CGRP) level as well as the expression of c-fos in trigeminal nucleus caudalis of rats. We found that NTG led to markedly increase in plasma CGRP level and c-fos expression in trigeminal nucleus caudalis compared with the isotonic saline-treated group (P < 0.05). More importantly, heat coagulation of middle meningeal artery could decrease plasma CGRP level and c-fos expression in trigeminal nucleus caudalis (P < 0.05). Heat coagulation of middle meningeal artery may ameliorate sufferings of rat induced by NTG and play an important role in restraining the release of CGRP as well as the activation of neurons in trigeminal nucleus caudalis in rats following NTG infusion.
authors.affiliation: Shandong University, Jinan, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhu
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Han
authors.forename: Wenping
authors.initials: W
authors.lastname: Xiong
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.forename: Sumei
authors.initials: S
authors.lastname: Lu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Fan
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000097:blood*; D017931:DNA Primers; D005260:Female; D006358:Hot Temperature; D008576:Meningeal Arteries / Q000502:physiology*; D008881:Migraine Disorders / Q000139:chemically induced* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D005996:Nitroglycerin; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013238:Stereotaxic Techniques; D014665:Vasodilator Agents
chemical_list: D000199:Actins; D017931:DNA Primers; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014665:Vasodilator Agents; D005996:Nitroglycerin; D015740:Calcitonin Gene-Related Peptide