title: Ultratrace enrichment of tyrosine phosphorylated peptides on an imprinted polymer.
abstract: Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO(2)-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics.
authors.affiliation: Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany. stefan.helling@rub.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Helling
authors.forename: Sudhirkumar
authors.initials: S
authors.lastname: Shinde
authors.forename: Frederic
authors.initials: F
authors.lastname: Brosseron
authors.forename: Anke
authors.initials: A
authors.lastname: Schnabel
authors.forename: Thorsten
authors.initials: T
authors.lastname: Müller
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.forename: Katrin
authors.initials: K
authors.lastname: Marcus
authors.forename: Börje
authors.initials: B
authors.lastname: Sellergren
mesh_terms: D010455:Peptides / Q000378:metabolism*; D010766:Phosphorylation; D011108:Polymers / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D010455:Peptides; D011108:Polymers; C009495:titanium dioxide; D014443:Tyrosine; D014025:Titanium