title: Study of prolactin permeation through the pericardium and its bioavailability.
abstract: The prolactin (PRL) permeation through the pericardium depending on the species of origin (porcine, bovine and ovine) was studied, and the parameters of its bioavailability were calculated. An in vitro model using pericardium as a natural membrane and Frantz cell method was applied. Significant differences in permeation were observed depending on the species of origin. Within 5 h, 17.5% of bovine PRL, 27.2% of porcine PRL and 90.3% of ovine PRL permeated the pericardium. The amount of permeated ovine PRL was 3.3-fold higher than porcine PRL and 5.2-fold higher than bovine PRL. The maximum concentration of permeated PRL was reached in the thirtieth minute of the experiment and was the highest for ovine PRL (C(max) = 677.21 µg/cm²) and the lowest for bovine PRL (C(max) = 259.97 µg/cm²). Bioavailability of PRL through the pericardium is 3.3-fold greater for ovine PRL in comparison to porcine or bovine PRL. The relative extent of bioavailability for bovine and ovine prolactin versus the porcine PRL standard was 85.6% and 229.3%, respectively.
authors.affiliation: Department of Applied Pharmacy, Medical University of Silesia, Sosnowiec, Poland. b.dolinska@biochefa.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Dolińska
authors.forename: Artur
authors.initials: A
authors.lastname: Caban
authors.forename: Lucyna
authors.initials: L
authors.lastname: Leszczyńska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Oczkowicz
authors.forename: Florian
authors.initials: F
authors.lastname: Ryszka
mesh_terms: D000818:Animals; D001682:Biological Availability; D002417:Cattle; D010496:Pericardium / Q000378:metabolism*; D010539:Permeability; D011388:Prolactin / Q000378:metabolism*; D012756:Sheep; D013045:Species Specificity; D013552:Swine
chemical_list: D011388:Prolactin