title: Remote focusing for programmable multi-layer differential multiphoton microscopy.
abstract: We present the application of remote focusing to multiphoton laser scanning microscopy and utilize this technology to demonstrate simultaneous, programmable multi-layer imaging. Remote focusing is used to independently control the axial location of multiple focal planes that can be simultaneously imaged with single element detection. This facilitates volumetric multiphoton imaging in scattering specimens and can be practically scaled to a large number of focal planes. Further, it is demonstrated that the remote focusing control can be synchronized with the lateral scan directions, enabling imaging in orthogonal scan planes.
authors.forename: Erich E
authors.initials: EE
authors.lastname: Hoover
authors.forename: Michael D
authors.initials: MD
authors.lastname: Young
authors.forename: Eric V
authors.initials: EV
authors.lastname: Chandler
authors.forename: Anding
authors.initials: A
authors.lastname: Luo
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Field
authors.forename: Kraig E
authors.initials: KE
authors.lastname: Sheetz
authors.forename: Anne W
authors.initials: AW
authors.lastname: Sylvester
authors.forename: Jeff A
authors.initials: JA
authors.lastname: Squier