title: [HPLC fingerprints of processed products from Sinapis alba].
abstract: OBJECTIVE
  To establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.
  
  
  METHODS
  The procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity.
  
  
  RESULTS
  The average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.
  
  
  CONCLUSIONS
  The established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.
authors.affiliation: Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Xiao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Pang
authors.forename: Guiliu
authors.initials: G
authors.lastname: Li
mesh_terms: D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031226:Sinapis / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal