title: [Simultaneous determination of five illegal drugs in weight control foods with solid phase extraction-high performance liquid chromatography].
abstract: OBJECTIVE
  To develop a method for simultaneous determination of hydrochlorothiazide, furosemide, clopamide, bumetanide and sibutramine hydrochloride in weight control foods with solid phase extraction-high performance liquid chromatography.
  
  
  METHODS
  The analytes in the samples were extracted with 2% phosphoric acid-methanol (1:1, V/V) solution ultrasonically and centrifuged. The extracts were clean-up with Osis MCX SPE columns, concentrated under weak N2 stream, and reconstituted with 2% phosphoric acid-methanol (1:1, V/V) solution, vortex mixing and centrifugation at 12,000 r/min. The high performance liquid chromatography was performed with Phenomenex C18 (250 x 4.60 mm, 5 microm) as separation column, 0.02 mol/L acetonitrile potassium dihydrogen phosphate buffer as mobile phase, gradient elution of 1.0 mL/min for the flow rate, and 40 degrees C for the column temperature. The standard curve method was used for the quantitative analysis.
  
  
  RESULTS
  A good linear range appeared for the five analytes from 0.25 to 100 microg/mL (r > or = 0.999). The detection limits were 5.2-108 microg/kg. The average recoveries were 86.5%-113.1%, with the relative standard deviations of 1.6%-8.9%.
  
  
  CONCLUSIONS
  The proposed method is a reliable method with high selectivity and high sensitivity for the detection of the five illegal chemicals in the weight control foods.
authors.affiliation: Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Zhang
authors.forename: Si-te
authors.initials: ST
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Cheng-jun
authors.initials: CJ
authors.lastname: Sun
mesh_terms: D002034:Bumetanide / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003005:Clopamide / Q000032:analysis*; D003503:Cyclobutanes / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005526:Food, Formulated / Q000032:analysis*; D005665:Furosemide / Q000032:analysis*; D006852:Hydrochlorothiazide / Q000032:analysis*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D003503:Cyclobutanes; D006852:Hydrochlorothiazide; D002034:Bumetanide; D003005:Clopamide; D005665:Furosemide; C058254:sibutramine