title: Ly6C supports preferential homing of central memory CD8+ T cells into lymph nodes.
abstract: Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells. We previously documented that Ly6C crosslinking induces LFA-1 clustering on naïve CD8(+) T cells. Here, we show that in vitro and in vivo differentiation of naïve CD8(+) T cells into central (Tcm) but not effector (Tem) memory T cells enhances Ly6C expression, and its crosslinking induces strong LFA-1 clustering on Tcm. Blocking Ly6C function inhibits in vivo Tcm homing to LNs as efficiently as blocking L-selectin but it does not potentiate the inhibition provided by blocking either L-selectin or LFA-1 function. Thus, Ly6C, L-selectin and LFA-1 all appear to be part of a common homing pathway. In vitro, Ly6C crosslinking enhances Tcm adherence to ICAM-1 in the presence of CCL21. In summary, Tcm homing involves Ly6C, in addition to L-selectin and LFA-1, and appears to potentiate firm adhesion of Tcm to ICAM-1 in synergy with a chemokine. We propose that Ly6C augments Tcm compartmentalization into LNs during their homing.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland. arno.hanninen@utu.fi
authors.forename: Arno
authors.initials: A
authors.lastname: Hänninen
authors.forename: Mikael
authors.initials: M
authors.lastname: Maksimow
authors.forename: Catharina
authors.initials: C
authors.lastname: Alam
authors.forename: David J
authors.initials: DJ
authors.lastname: Morgan
authors.forename: Sirpa
authors.initials: S
authors.lastname: Jalkanen
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000737:chemistry / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D002465:Cell Movement / Q000276:immunology; D003432:Cross-Linking Reagents; D007156:Immunologic Memory; D066298:In Vitro Techniques; D019041:L-Selectin / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000276:immunology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology; D015854:Up-Regulation
chemical_list: D000950:Antigens, Ly; D003432:Cross-Linking Reagents; C494192:Ly-6C antigen, mouse; D016169:Lymphocyte Function-Associated Antigen-1; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin