title: A malaria gametocytocidal assay using oxidoreduction indicator, alamarBlue.
abstract: Efforts to move from malaria control to eradication will require new approaches to block malaria transmission, such as the development of anti-malarial drugs with gametocytocidal activity. Here fluorescent oxidoreduction indicator alamarBlue is used to develop a screen for gametocyte viability. The fluorescent signal increases linearly with gametocyte number (R(2)=0.99) and determination of the IC(50) of epoxomicin demonstrated the assay was reproducible and sensitive (IC(50) 2.16±0.57 nM, Z'-factor 0.81±0.01). Six anti-malarials were also tested and at 10 μM only primaquine and dihydroartemisinin (DHA) had gametocytocidal activity. This new assay provides an important tool to efficiently screen compounds for gametocytocidal activity.
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20892, United States.
authors.forename: Takeshi Q
authors.initials: TQ
authors.lastname: Tanaka
authors.forename: Kim C
authors.initials: KC
authors.lastname: Williamson
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D037621:Artemisinins / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D020128:Inhibitory Concentration 50; D009842:Oligopeptides / Q000494:pharmacology; D010078:Oxazines / Q000378:metabolism*; D010084:Oxidation-Reduction; D021261:Parasitic Sensitivity Tests / Q000379:methods; D010961:Plasmodium / Q000187:drug effects*; D011319:Primaquine / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods*; D014966:Xanthenes / Q000378:metabolism*
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D009842:Oligopeptides; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; C039060:artenimol; D011319:Primaquine; C078846:epoxomicin