title: Osthole, a natural coumarin, improves neurobehavioral functions and reduces infarct volume and matrix metalloproteinase-9 activity after transient focal cerebral ischemia in rats.
abstract: Previously we demonstrated that Osthole, a natural coumarin, protects against focal cerebral ischemia/reperfusion-induced injury in rats. In the present study, the effects of Osthole on neurobehavioral functions, infarct volume and matrix metalloproteinase-9 (MMP-9) in a rat 2h focal cerebral ischemia model were investigated. Osthole (100mg/kg per dose) was administrated intraperitoneally 30min before ischemic insult and immediately after reperfusion. Osthole treatment significantly reduced neurological deficit score and infarct volume by 38.5% and 33.8%, respectively, as compared with the untreated animals. Osthole reversed ischemia-reperfusion-induced increase in MMP-9 protein level/activity as evidenced by Western blotting and gelatin zymography. Taken together, these results for the first time demonstrate that Osthole reduces infarct volume, restores neurobehavioral functions and downregulates MMP-9 protein level/activity in ischemia/reperfused brain.
authors.affiliation: Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Xuexuan
authors.initials: X
authors.lastname: Mao
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Mengfei
authors.initials: M
authors.lastname: Liu
authors.forename: Minzhong
authors.initials: M
authors.lastname: Ye
authors.forename: Peiqing
authors.initials: P
authors.lastname: Liu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
authors.forename: Qishen
authors.initials: Q
authors.lastname: Lian
authors.forename: Suowen
authors.initials: S
authors.lastname: Xu
authors.forename: Rongbiao
authors.initials: R
authors.lastname: Pi
mesh_terms: D000818:Animals; D002544:Cerebral Infarction / Q000201:enzymology / Q000473:pathology* / Q000517:prevention & control*; D003374:Coumarins / Q000494:pharmacology / Q000627:therapeutic use*; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D002546:Ischemic Attack, Transient / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000502:physiology; D061965:Matrix Metalloproteinase Inhibitors; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003374:Coumarins; D061965:Matrix Metalloproteinase Inhibitors; D020780:Matrix Metalloproteinase 9; C046627:osthol