title: Rapid characterization of sugar-binding specificity by in-solution proximity binding with photosensitizers.
abstract: Cell-surface carbohydrates are known to participate in many important physiological and pathological activities by interacting with their corresponding proteins or receptors. Although several methods have been developed for studying carbohydrate-protein interactions, one major problem originates from the weak bindings of carbohydrates/proteins that are often lost during repeating wash steps. Herein, we established a homogeneous solution carbohydrate array in which polyacrylamide-based glycans are used for offering a multivalent environment. The method requires no wash step and can be carried out in a high-throughput manner. We characterized the carbohydrate-binding specificities of 11 lectins and 7 antibodies, the majority of which displayed the binding patterns in consistence with previous reports. These results demonstrate that our developed solution carbohydrate array provides a useful alternative that is better than or comparable with the current available methods.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, National Taiwan University, Taipei.
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Chang
authors.forename: Jia-Fu
authors.initials: JF
authors.lastname: Pan
authors.forename: Chun-Nan
authors.initials: CN
authors.lastname: Lin
authors.forename: I-Lin
authors.initials: IL
authors.lastname: Wu
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Lin
mesh_terms: D000180:Acrylic Resins / Q000378:metabolism; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis; D017319:Photosensitizing Agents; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000906:Antibodies; D037102:Lectins; D017319:Photosensitizing Agents; D011134:Polysaccharides; D011506:Proteins; D000099166:Polyacrylamides