title: Fast preparation of fungal DNA for PCR screening.
abstract: Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
authors.forename: Kuang-Hung
authors.initials: KH
authors.lastname: Liu
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Yeh
authors.forename: Wei-Chiang
authors.initials: WC
authors.lastname: Shen
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D005658:Fungi / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009181:Mycoses / Q000382:microbiology; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal