title: Reliable enzyme-linked immunosorbent assay for the determination of coconut milk proteins in processed foods.
abstract: This study was designed to develop a novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of coconut milk proteins in processed foods. The developed sandwich ELISA was able to detect coconut milk proteins from various coconut milk products and did not show any cross-reactivity with 41 of 42 kinds of popularly used food ingredients, thus reflecting great specificity for coconut milk proteins. In addition, the established ELISA is highly sensitive and allowed the detection of 0.31 μg/g of coconut milk protein in complex food matrices. This proposed assay could serve as a useful tool for the detection of the presence of hidden coconut milk proteins in processed foods.
authors.affiliation: Institute of Food Research and Product Development (IFRPD), Kasetsart University, 10900, Bangkok, Thailand. ifrvis@ku.ac.th
authors.forename: Vipa
authors.initials: V
authors.lastname: Surojanametakul
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Doi
authors.forename: Haruki
authors.initials: H
authors.lastname: Shibata
authors.forename: Tasuku
authors.initials: T
authors.lastname: Mizumura
authors.forename: Toshio
authors.initials: T
authors.lastname: Takahashi
authors.forename: Warunee
authors.initials: W
authors.lastname: Varanyanond
authors.forename: Sirinrat
authors.initials: S
authors.lastname: Wannapinpong
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shoji
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Ito
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Tamura
mesh_terms: D052179:Antigens, Plant / Q000032:analysis; D003059:Cocos / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057140:Fast Foods / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D052179:Antigens, Plant; D010940:Plant Proteins