title: Determination of clomazone residues in soybean and soil by high performance liquid chromatography with DAD detection.
abstract: A simple analysis method to detect clomazone residues in soybean and soil was developed using solid phase extraction coupled with high performance liquid chromatography with diode-array detection. The pesticide residues present in soybean and soil matrices were extracted with methanol-water and extracts purified with Florisil cartridges. The analytes from soybean and soil matrix were eluted with petroleum ether-acetic ether (10 mL, 95:5, v/v) and petroleum ether-acetic ether (2 mL, 95:5, v/v), respectively. The overall recovery of fortified soybean and soil at the levels of 0.01, 0.1 and 0.5 mg/kg ranged from 89.75% to 106.6%, and the coefficients of variation (CV) ranged from 1.68% to 4.93% (n = 3). The limit of quantification (LOQ) is 0.01 mg/kg. This method has been applied to the analysis of clomazone in real samples of soybean and soil. The dissipation of residue over the time in soil coincided with C = 1.189e(-0.0926t ) and the half-lives (T(1/2)) was 7.48 days. The final residue in soybean was lower than 0.01 mg/kg at harvest time. Direct confirmation of the analyte in real samples was achieved by gas chromatography-mass spectrometry.
authors.affiliation: School of Chemical and Biological Engineering, University of Science and Technology Beijing, Beijing, People's Republic of China. jyhu@ustb.edu.cn
authors.forename: Jiye
authors.initials: J
authors.lastname: Hu
authors.forename: Di
authors.initials: D
authors.lastname: Cao
authors.forename: Zhubo
authors.initials: Z
authors.lastname: Deng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D006540:Herbicides / Q000032:analysis* / Q000737:chemistry; D007555:Isoxazoles / Q000032:analysis* / Q000737:chemistry; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D052616:Solid Phase Extraction; D013025:Glycine max / Q000737:chemistry*
chemical_list: D006540:Herbicides; D007555:Isoxazoles; D023303:Oxazolidinones; D010573:Pesticide Residues; D012987:Soil; D012989:Soil Pollutants; C095255:clomazone