title: Adenovirus vector-mediated assay system for hepatitis C virus replication.
abstract: The efficient delivery of the hepatitis C virus (HCV) RNA subgenomic replicon into cells is useful for basic and pharmaceutical studies. The adenovirus (Ad) vector is a convenient and efficient tool for the transduction of foreign genes into cells in vitro and in vivo. However, an Ad vector expressing the HCV replicon has never been developed. In the present study, we developed Ad vector containing an RNA polymerase (pol) I-dependent expression cassette and a tetracycline-controllable RNA pol I-dependent expression system. We prepared a hybrid promoter from the tetracycline-responsive element and the RNA pol I promoter. Ad vector particles coding the hybrid promoter-driven HCV replicon could be amplified, and interferon, an inhibitor of HCV replication, reduced HCV replication in cells transduced with the Ad vector coding HCV replicon. This is the first report of the development of an Ad vector-mediated HCV replicon system.
authors.affiliation: Laboratory of Bio-Functional Molecular Chemistry, Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masuo
authors.initials: M
authors.lastname: Kondoh
authors.forename: Manabu
authors.initials: M
authors.lastname: Ojima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamagishi
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D017930:Genes, Reporter; D005821:Genetic Techniques; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000378:metabolism; D012093:Replicon; D014779:Virus Replication
chemical_list: D008156:Luciferases; D012318:RNA Polymerase I