title: Solubilization and structural stability of bacteriorhodopsin with a mild nonionic detergent, n-Octyl-β-thioglucoside.
abstract: Solubilization and structural stability of a membrane protein bacteriorhodopsin (bR) with n-octyl-β-thioglucoside (OTG) was investigated in comparison with a previous study on bR solubilized with n-octyl-β-glucoside (OG). Highly efficient and stable solubilization of bR with OTG was accomplished above the OTG concentration of about 15 mM. In comparison with OG-solubilized bR, the structural stability of OTG-solubilized bR was high in the dark and under light illumination. These results indicate that OTG is a detergent superior to OG for solubilizing bR molecules.
authors.affiliation: Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Gunma, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Asada
authors.forename: Masashi
authors.initials: M
authors.lastname: Sonoyama
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019617:Halobacterium salinarum; D008823:Micelles; D055550:Protein Stability / Q000187:drug effects; D012995:Solubility / Q000187:drug effects; D013863:Thioglucosides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D003902:Detergents; D008823:Micelles; D013863:Thioglucosides; D001436:Bacteriorhodopsins; C042989:n-octyl-beta-D-thioglucopyranoside