title: Relative concentrations of haemostatic factors and cytokines in solvent/detergent-treated and fresh-frozen plasma.
abstract: BACKGROUND
  Indications, efficacy, and safety of plasma products are highly debated. We compared the concentrations of haemostatic proteins and cytokines in solvent/detergent-treated plasma (SDP) and fresh-frozen plasma (FFP).
  
  
  METHODS
  Concentrations of the following parameters were measured in 25 SDP and FFP samples: fibrinogen (FBG), factor (F) II, F V, F VII, F VIII, F IX, F X, F XIII, von Willebrand factor (vWF), D-Dimers, ADAMTS-13 protease, tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, and IL-10.
  
  
  RESULTS
  Mean FBG concentrations in SDP and FFP were similar, but in FFP, the range was larger than in SDP (P<0.01). Mean F II, F VII, F VIII, F IX, and F XIII levels did not differ significantly. Higher concentrations of F V (P<0.01), F X (P<0.05), vWF (P<0.01), and ADAMTS-13 (P<0.01) were found in FFP. With the exception of F VIII and F IX, the range of concentrations for all of these factors was smaller (P<0.05) in SDP than in FFP. Concentrations of TNF-α, IL-8, and IL-10 (all P<0.01) were higher in FFP than in SDP, again with a higher variability and thus larger ranges (P<0.01).
  
  
  CONCLUSIONS
  Coagulation factor content is similar for SDP and FFP, with notable exceptions of less F V, vWF, and ADAMTS-13 in SDP. Cytokine concentrations (TNFα, IL-8, and IL-10) were significantly higher in FFP. The clinical relevance of these findings needs to be established in outcome studies.
authors.affiliation: Institute of Anaesthesiology, University Hospital Zurich, Zurich, Switzerland. oliver.theusinger@usz.ch
authors.forename: O M
authors.initials: OM
authors.lastname: Theusinger
authors.forename: W
authors.initials: W
authors.lastname: Baulig
authors.forename: B
authors.initials: B
authors.lastname: Seifert
authors.forename: M Y
authors.initials: MY
authors.lastname: Emmert
authors.forename: D R
authors.initials: DR
authors.lastname: Spahn
authors.forename: L M
authors.initials: LM
authors.lastname: Asmis
mesh_terms: D001779:Blood Coagulation Factors / Q000032:analysis*; D016207:Cytokines / Q000097:blood*; D003902:Detergents / Q000494:pharmacology*; D006487:Hemostasis; D006801:Humans; D010949:Plasma / Q000737:chemistry / Q000187:drug effects*; D012016:Reference Values; D012997:Solvents / Q000494:pharmacology*
chemical_list: D001779:Blood Coagulation Factors; D016207:Cytokines; D003902:Detergents; D012997:Solvents