title: Determination of (3)J((1)H3'-(31)P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment.
abstract: A constant-time TOCSY difference experiment for the determination of (3)J((1)H3'-(31)P) coupling constants in non-isotope-labelled DNA oligonucleotides is presented. The method is tested on a DNA octamer and compared with the established constant-time NOESY difference method. Each (3)J((1)H3'-(31)P) coupling constant is determined from amplitude changes caused by phosphorous decoupling, which are observable on multiple cross-peaks, thus leading to a high accuracy of the value of the (3)J((1)H3'-(31)P) coupling constant. The new experiment delivers up to three times the sensitivity compared with previously reported methods.
authors.affiliation: Institute of Inorganic Chemistry, Johannes Kepler University, Linz, Austria.
authors.forename: Lorenz M
authors.initials: LM
authors.lastname: Reith
authors.forename: Judith
authors.initials: J
authors.lastname: Schlagnitweit
authors.forename: Vilko
authors.initials: V
authors.lastname: Smrecki
authors.forename: Günther
authors.initials: G
authors.lastname: Knör
authors.forename: Norbert
authors.initials: N
authors.lastname: Müller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schoefberger
mesh_terms: D004247:DNA / Q000737:chemistry*; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D004247:DNA