title: Possible mechanism of preventive effects of coffee intake on the formation of arterial occlusive thrombosis.
abstract: BACKGROUND
  Prevalence and incidence of arterial occlusive thrombosis are influenced by life-style. Coffee consumption was shown with a lower incidence of myocardial infarction by Framingham Study. Yet, the mechanism is to be elucidated.
  
  
  METHODS
  We examined the effects of coffee intake on the progression of occlusive thrombus formation in mouse cremasteric arteries. After 7 days of free intake of pure water, coffee containing water (5 mg/ml), or caffeine containing water (0.1 mg/ml), endothelial cell function was locally damaged by FeCl3. Circulating platelet and leukocytes were rendered fluorescently by rhodamine 6G. Process of occlusive thrombus growth was continuously visualized by 3-D imaging system equipped with ultra-fast confocal microscopy, and time to vascular occlusion was measured in each mouse.
  
  
  RESULTS
  Platelet accumulation started immediately after FeCl3 exposure in all tested groups. However, arterial occlusion time in taking coffee containing water was significantly longer than those taking pure water. (46.0 ± 17.4 min (n = 5) vs. 12.3 ± 2.6 min (n = 31), p < 0.05) Arterial occlusion time in mice taking caffeine (13.8 ± 5.9 min (n = 4)) was not different from those taking pure water.
  
  
  CONCLUSIONS
  Coffee, but not caffeine intake, may have preventive effect on arterial occlusive thrombus formation initiated by functional injury of arterial endothelium.
authors.affiliation: Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Toda
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Ishida
authors.forename: Takuya
authors.initials: T
authors.lastname: Aoki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Urano
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahari
authors.forename: Noriko
authors.initials: N
authors.lastname: Tamura
authors.forename: Shinya
authors.initials: S
authors.lastname: Goto
mesh_terms: D000818:Animals; D001158:Arteries / Q000033:anatomy & histology / Q000187:drug effects* / Q000473:pathology*; D002110:Caffeine / Q000494:pharmacology / Q000627:therapeutic use; D002712:Chlorides / Q000494:pharmacology; D003069:Coffee / Q000737:chemistry; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods; D058085:Iron Compounds / Q000494:pharmacology; D008297:Male; D051379:Mice; D013927:Thrombosis / Q000139:chemically induced / Q000517:prevention & control*; D014867:Water / Q000494:pharmacology
chemical_list: D002712:Chlorides; D003069:Coffee; D058085:Iron Compounds; D014867:Water; D002110:Caffeine