title: Real-time polymerase chain reaction detection of fishmeal in feedstuffs.
abstract: A SYBR Green PCR system was developed for detection of fishmeal in feedstuffs. The real-time PCR method combines the use of fish-specific primers that amplify an 87 base pair (bp) fragment of the mitochondrial 12S ribosomal RNA gene from fish species, and a positive control primer pair that amplifies a 99 bp fragment of the nuclear 18S ribosomal RNA gene in all eukaryotic organisms. The specificity of the primers was tested against 52 animal species and six plant species. Reference feedstuff samples were successfully tested for the presence of fishmeal, demonstrating the applicability of the assay to feedstuffs.
authors.affiliation: Universidad Complutense de Madrid, Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, 28040 Madrid, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Martín
authors.forename: Teresa
authors.initials: T
authors.lastname: García
authors.forename: Maria
authors.initials: M
authors.lastname: Rojas
authors.forename: Nicolette
authors.initials: N
authors.lastname: Pegels
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Pavón
authors.forename: Pablo E
authors.initials: PE
authors.lastname: Hernández
authors.forename: Isabel
authors.initials: I
authors.lastname: González
authors.forename: Rosario
authors.initials: R
authors.lastname: Martín
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000737:chemistry; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D005396:Fish Products / Q000032:analysis*; D008460:Meat / Q000032:analysis; D010944:Plants / Q000737:chemistry; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D018744:DNA, Plant; D004247:DNA