title: Mucilaginibacter angelicae sp. nov., isolated from the rhizosphere of Angelica polymorpha Maxim.
abstract: A Gram-negative-staining, non-motile rod, designated GG-w14(T), was isolated from the rhizosphere of Angelica polymorpha Maxim. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolate belonged to the genus Mucilaginibacter and exhibited 93.9-97.4% 16S rRNA gene sequence similarity with recognized members of the genus Mucilaginibacter (closest relative Mucilaginibacter gossypii Gh-67(T)). DNA-DNA relatedness between strain GG-w14(T) and M. gossypii KCTC 22380(T) was <41%. Strain GG-w14(T) grew at 4-35 °C, at pH 5.0-8.0 and with 0-1% (w/v) NaCl. The isolate hydrolysed casein, CM-cellulose and starch and contained menaquinone 7 as the major menaquinone. The major cellular fatty acids were summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH; 39.9%), iso-C(15:0) (24.2%) and iso-C(17:0) 3-OH (12.4%). The DNA G+C content was 42.5 mol%. These data suggest that strain GG-w14(T) should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter angelicae sp. nov. is proposed. The type strain is GG-w14(T) (=KCTC 23250(T)=NCAIM B 02415(T)).
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Byung-Chun
authors.initials: BC
authors.lastname: Kim
authors.affiliation: Medical Genomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Haryoung
authors.initials: H
authors.lastname: Poo
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Kim
authors.affiliation: Department of Anatomy, College Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: O-Yu
authors.initials: OY
authors.lastname: Kwon
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
mesh_terms: D029969:Angelica / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride