title: Correlative study of indirect computed tomography lymphography using iopamidol and histopathology in a cervical lymph node metastasis model.
abstract: OBJECTIVE
  To investigate the potential usefulness of indirect computed tomography lymphography (CT-LG) with iopamidol in the detection of cervical lymph node metastases in a VX2 rabbit tongue cancer model.
  
  
  METHODS
  Randomized controlled trial.
  
  
  METHODS
  Thirty-seven rabbits were randomly divided into control (n = 7) and tongue tumor-bearing (n = 30) groups. In the control group, indirect CT-LG was performed with 0.5- and 0.25-mL injections of iopamidol in the right and left tongue submucosa, respectively. In the tumor-bearing group, indirect CT-LG with 0.5 mL of iopamidol was performed on day 11 (n = 8), 14 (n = 8), 21 (n = 8), or 28 (n = 6) after carcinoma transplant. The correlation between indirect CT-LG and histopathology was investigated.
  
  
  RESULTS
  In normal rabbits, oval or round enhanced cervical lymph nodes in each side of the neck were visualized by indirect CT-LG. In tumor-bearing rabbits, one enhanced lymph node on each side of the neck was visualized with or without filling defects in all rabbits except two on day 28 in which lymph nodes were not enhanced. The sensitivity, specificity, accuracy, and positive and negative predictive values for using filling defects on CT-LG as a diagnostic criterion were 77.4%, 77.8%, 77.6%, 80%, and 75%, respectively. Indirect CT-LG detected 84.6% of 2- to 6-mm intranodal tumor deposits.
  
  
  CONCLUSIONS
  Indirect CT-LG may be useful for differentiating metastatic from nonmetastatic lymph nodes.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, EENT Hospital, Fudan University, Shanghai, China.
authors.forename: Yilai
authors.initials: Y
authors.lastname: Shu
authors.forename: Xiuyin
authors.initials: X
authors.lastname: Xu
authors.forename: Ann M
authors.initials: AM
authors.lastname: Chodara
authors.forename: Michael F
authors.initials: MF
authors.lastname: Regner
authors.forename: Yan
authors.initials: Y
authors.lastname: Sha
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Jiang
authors.forename: Mingliang
authors.initials: M
authors.lastname: Xiang
authors.forename: Haitao
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage*; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007479:Iopamidol; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging*; D008220:Lymphography / Q000379:methods*; D009368:Neoplasm Transplantation; D011817:Rabbits; D012680:Sensitivity and Specificity; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014062:Tongue Neoplasms / Q000000981:diagnostic imaging*
chemical_list: D003287:Contrast Media; D007479:Iopamidol