title: How Förster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology.
abstract: Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits (nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.
authors.affiliation: Richard Dimbleby Department of Cancer Research, Division of Cancer Studies, King's College London, Guy's Medical School Campus, NHH, SE1 1UL, UK. gilbert.fruhwirth@kcl.ac.uk
authors.forename: Gilbert O
authors.initials: GO
authors.lastname: Fruhwirth
authors.forename: Luis P
authors.initials: LP
authors.lastname: Fernandes
authors.forename: Gregory
authors.initials: G
authors.lastname: Weitsman
authors.forename: Gargi
authors.initials: G
authors.lastname: Patel
authors.forename: Muireann
authors.initials: M
authors.lastname: Kelleher
authors.forename: Katherine
authors.initials: K
authors.lastname: Lawler
authors.forename: Adrian
authors.initials: A
authors.lastname: Brock
authors.forename: Simon P
authors.initials: SP
authors.lastname: Poland
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Matthews
authors.forename: György
authors.initials: G
authors.lastname: Kéri
authors.forename: Paul R
authors.initials: PR
authors.lastname: Barber
authors.forename: Borivoj
authors.initials: B
authors.lastname: Vojnovic
authors.forename: Simon M
authors.initials: SM
authors.lastname: Ameer-Beg
authors.forename: Anthony C C
authors.initials: AC
authors.lastname: Coolen
authors.forename: Franca
authors.initials: F
authors.lastname: Fraternali
authors.forename: Tony
authors.initials: T
authors.lastname: Ng
mesh_terms: D019295:Computational Biology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins