title: [Determination of flavones in different origin and parts of Bupleurum smithii var. parvifoliaum by UPLC-PDA].
abstract: An ultra performance liquid chromatography method has been developed for determination of flavones in different origin and different parts from Bupleurum smithii var. parvifoliaum. The separation was performed at 30 degrees C on an Acquity UPLC BEH C18 column eluted with methanol and 0.1% phosphoric acid solution as mobile phases in gradient elution. The detection wavelength was set at 256 nm and the flow rate was 0.4 mL x min(-1). There flavones of rutin, quercetin and isorhamnetin were separated well, the linear calibration curves were obtained over the ranges of 0.106-1.06 microg for rutin (r = 0.999 8, n = 6), 0.004 87-0.048 7 microg for quercetin (r = 0. 999 7, n = 6), 0.022 0-0.220 microg for isorhamnetin (r = 0.999 8, n = 6). The mean recoveries of the three compounds were 99.3%, 97.8%, 98.9% with RSD of 2.4%, 3.0%, 3.2%. The result showed that the method is convenient and feasible for determination of the flavone content in B. smithii var. parvifoliaum.
authors.affiliation: China Military Institute of Chinese Materia Mdeica, 302 Military Hospital, Beijing 100039, China.
authors.forename: Fangling
authors.initials: F
authors.lastname: Tang
authors.forename: Guangming
authors.initials: G
authors.lastname: Cai
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.forename: Zhuoyong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D047309:Flavones / Q000032:analysis*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones