title: The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells.
abstract: Human leukemia inhibitory factor (LIF) was immobilized into insect virus-derived microcrystals (polyhedra) to generate LIF polyhedra (LIF-PH) that can slowly release LIF into embryonic stem (ES) cell culture media and thus maintain ES cells in an undifferentiated state. Assays of the biological activities of LIF-PH indicated that a single addition of LIF-PH to the ES cell culture medium can support the proliferation of mouse ES and induced pluripotent stem (iPS) cells continuously for 14 days, and suggest that LIF-PH can be successfully used in the place of a periodic addition of recombinant LIF to the media every 2-3 days. The release of LIF protein from LIF-PH was determined by enzyme-linked immunosorbent assay (ELISA). Maintenance of undifferentiated state of mouse ES and iPS cells cultured with LIF-PH was determined by the detection of pluripotency-related biomarkers Oct3/4 and stage-specific embryonic antigen-1 (SSEA-1) through immunostaining and measurement of alkaline phosphatase activity. In this paper, we propose a closed culture system for mass production of ES and iPS cells that utilize a slow-releasing agent of LIF.
authors.affiliation: Foundation for Biomedical Research and Innovation TRI308, 1-5-4 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0043, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Nishishita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ijiri
authors.forename: Chiemi
authors.initials: C
authors.lastname: Takenaka
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kohei
authors.initials: K
authors.lastname: Goto
authors.forename: Eiji
authors.initials: E
authors.lastname: Kotani
authors.forename: Tohru
authors.initials: T
authors.lastname: Itoh
authors.forename: Hajime
authors.initials: H
authors.lastname: Mori
authors.forename: Shin
authors.initials: S
authors.lastname: Kawamata
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects*; D053605:Leukemia Inhibitory Factor / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D050796:STAT3 Transcription Factor / Q000378:metabolism
chemical_list: D053605:Leukemia Inhibitory Factor; D050796:STAT3 Transcription Factor