title: Testicular apoptosis and histopathological changes induced by a 2.45 GHz electromagnetic field.
abstract: There is a growing public concern about the potential human health hazard caused by exposure to electromagnetic radiation (EMR). The objective of this study is to investigate the effects of 2450 mhz electromagnetic field on apoptosis and histopathological changes on rat testis tissue. Twelve-week-old male Wistar Albino rats were used in this study. Eighteen rats equally divided into three different groups which were named group I, II and III. Cage control (group I), sham control (group II) and 2.45 GHz EMR (group III) groups were formed. Group III were exposed to 2.45 GHz EMR, at 3.21 W/kg specific absorption rate for 60 minutes/ day for 28 days. There was no difference among the groups for the diameter of the seminiferous tubules, pyknotic, karyolectic and karyotic cells. However, the number of Leydig cells of testis tissue of the rats in group III was significantly reduced comparing with the group I (p < 0.05). Estimation of spermatogenesis using the Johnsen testicular biopsy score revealed that the difference between groups is statistically significant. The level of TNF-α, Caspase-3 and Bcl-2 were compared, and no significant difference was found between the groups. When Bax apoptosis genes and Caspase-8 apoptosis enzyme were compared, there were significant differences between the groups (p < 0.05). Electromagnetic field affects spermatogenesis and causes to apoptosis due to the heat and other stress-related events in testis tissue.
authors.affiliation: Department of Physiology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey. msaygin@med.sdu.edu.tr
authors.forename: M
authors.initials: M
authors.lastname: Saygin
authors.forename: S
authors.initials: S
authors.lastname: Caliskan
authors.forename: N
authors.initials: N
authors.lastname: Karahan
authors.forename: A
authors.initials: A
authors.lastname: Koyu
authors.forename: N
authors.initials: N
authors.lastname: Gumral
authors.forename: Ac
authors.initials: A
authors.lastname: Uguz
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000032:analysis; D053181:Caspase 8 / Q000032:analysis; D004574:Electromagnetic Fields / Q000009:adverse effects*; D007150:Immunohistochemistry / Q000379:methods; D007985:Leydig Cells / Q000166:cytology / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000473:pathology / Q000528:radiation effects; D013091:Spermatogenesis / Q000528:radiation effects; D013733:Testicular Diseases / Q000473:pathology; D013737:Testis / Q000473:pathology* / Q000528:radiation effects*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: C494828:Bax protein, rat; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; C505528:Casp8 protein, rat; D053148:Caspase 3; D053181:Caspase 8