title: Interferon-gamma low producer genotype +5644 over presented in patients with focal brucellosis.
abstract: Genetic polymorphisms that affect production levels of certain cytokines may determine the risk, severity or protection in some infectious diseases like brucellosis. IFN-gamma plays a key role in the defense mechanism against brucella infection. This study aimed to determine the influence of the polymorphism within the +5644 position of IFN-gamma gene on the susceptibility to brucellosis. We investigated the allelic and genotypes distribution of A5644G polymorphism in IFN-gamma gene in an Iranian population comprising 259 patients with brucellosis and 238 healthy controls. The single nucleotide polymorphism was determined using the polymerase chain reaction in association with sequence-specific primers (PCR-SSP) incorporating mismatches at the 3'-end. Allelic and genotype frequencies of G5644A polymorphism of IFN-gamma gene were not significantly differed between patients with brucellosis and controls (p > 0.05). Stratification of patients to focal and non focal diseases revealed a significant increased of 5644A allele in patients with focal brucellosis (79.31% vs. 61.94%, p = 0.0005). Moreover, multivariate logistic regression models showed patients harboring the INF-y G5644A genotype were significantly more likely to develop focal infectious complications (OR = 3.45, p = 0.0004, 95% CI = 1.26-7.94). The present study suggests that the variant genotypes of G6544A of IFN-gamma might be associated with focal form of brucellosis and play as a genetic risk factor in brucellosis.
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Hedayatizadeh-Omran
authors.forename: A
authors.initials: A
authors.lastname: Rafiei
authors.forename: M
authors.initials: M
authors.lastname: Hajilooi
authors.forename: M
authors.initials: M
authors.lastname: Haghshenas
mesh_terms: D000328:Adult; D000368:Aged; D002006:Brucellosis / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D007371:Interferon-gamma