title: Effect of the number of rol genes integrations on phenotypic variation in hairy root-derived Hypericum perforatum L. plants.
abstract: The extent of phenotypic variation of St. John's wort (Hypericum perforatum L.) plants transformed with wild agropine ATCC 15834 Agrobacterium rhizogenes plasmid was evaluated with respect to the number of rol genes integrations. The transfer of T(L)-DNA to plant explants during each transformation event was incomplete with different rolA, rolB, and rolC copy numbers. Along with typical features representing the hairy root syndrome, an altered size, number and density of dark and translucent glands, changes in ability to synthesize secondary metabolites, and reduced fertility were observed. The highest copy number of transferred rol genes resulted in weak expression of transgenic character and comparable quantitative parameters with the controls. Only 1 out of 11 transgenic clones was able to produce seed progeny and not more than 4 out of its 35 offsprings were positive for rolC gene integration. Sterility of the clones was due to retarded development of both gametophytes.
authors.affiliation: P. J. Safárik University, Faculty of Science, Institute of Biology and Ecology, Mánesova 23, 041 54 Kosice, Slovakia.
authors.forename: Hedviga
authors.initials: H
authors.lastname: Komarovská
authors.forename: Ján
authors.initials: J
authors.lastname: Kosuth
authors.forename: Annalisa
authors.initials: A
authors.lastname: Giovannini
authors.forename: Andrija
authors.initials: A
authors.lastname: Smelcerovic
authors.forename: Sebastian
authors.initials: S
authors.lastname: Zuehlke
authors.forename: Eva
authors.initials: E
authors.lastname: Cellárová
mesh_terms: D000222:Adaptation, Physiological; D001483:Base Sequence; D017931:DNA Primers; D017343:Genes, Plant; D020902:Hypericum / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000378:metabolism*; D030821:Plants, Genetically Modified
chemical_list: D017931:DNA Primers