title: Transgenic rodents with altered SSAT expression as models of pancreatitis and altered glucose and lipid metabolism.
abstract: Depletion of pancreatic acinar cell polyamines in response to activation of polyamine catabolism is associated with the development of acute pancreatitis in experimental rodent models. The disease is characterized by general hallmarks seen also in human pancreatitis, such as accumulation of intraperitoneal ascites, acinar cell necrosis, and pancreatic as well as remote organ edema and inflammation. Thus, these animals make useful models for the human disease. Determination of these hallmarks can be used to assess the severity of the disease and to evaluate the efficacy of any therapy applied. The metabolic changes seen in genetically modified mice with either accelerated or inactivated polyamine catabolism have revealed that polyamine catabolism is involved in the regulation of glucose and lipid metabolism. The simplest method to determine the metabolic phenotype of the animal is to assess the concentrations of blood metabolites. Fasting blood glucose level is an indicator of overall glucose homeostasis, whereas fasting insulin level is a useful marker of insulin sensitivity. A more detailed analysis of glucose homeostasis and insulin sensitivity can be obtained by intraperitoneal glucose and insulin tolerance tests. Blood lipid levels mainly reflect triglyceride, free fatty acid, and cholesterol metabolism. Altered blood glucose and/or lipid levels are associated with several diseases, e.g., diabetes, Cushing's syndrome, hyperthyroidism, atherosclerosis, pancreatitis, and dysfunction of the liver and kidneys.
authors.affiliation: A.I. Virtanen Institute for Molecular Sciences, Biocenter Kuopio, University of Kuopio, Kuopio, Finland.
authors.forename: Anne
authors.initials: A
authors.lastname: Uimari
authors.forename: Mervi T
authors.initials: MT
authors.lastname: Hyvönen
authors.forename: Eija
authors.initials: E
authors.lastname: Pirinen
authors.forename: Leena
authors.initials: L
authors.lastname: Alhonen
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004195:Disease Models, Animal; D004487:Edema / Q000473:pathology; D057075:Enzyme Assays; D005215:Fasting / Q000097:blood; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D006801:Humans; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000097:blood; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D051379:Mice; D008822:Mice, Transgenic; D010195:Pancreatitis / Q000097:blood / Q000201:enzymology* / Q000473:pathology*; D009195:Peroxidase / Q000378:metabolism; D013194:Staining and Labeling; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D001786:Blood Glucose; D005947:Glucose; D007328:Insulin; D008055:Lipids; D009195:Peroxidase; D000099228:Diamine N-Acetyltransferase