title: Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX.
abstract: We applied Systematic Evolution of Ligands by EXponential enrichment using Small Cell Lung Cancer (SCLC) cells. A DNA aptamer was identified and evaluated by fluorescent confocal microscopy and flow cytometry. Our results showed that the DNA aptamer binds to molecules that exist predominantly on target SCLC cell surfaces compared with other types of SCLC cells.
authors.affiliation: Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Kunii
authors.forename: Shun-ichiro
authors.initials: S
authors.lastname: Ogura
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D018613:Microscopy, Confocal; D052156:SELEX Aptamer Technique / Q000379:methods*; D055752:Small Cell Lung Carcinoma / Q000737:chemistry* / Q000473:pathology
chemical_list: D052157:Aptamers, Nucleotide; D016650:Fluorescein-5-isothiocyanate