title: Metabolic correlates of tumour hypoxia in malignant canine mammary carcinoma.
abstract: Given its importance in human and canine tumour biology, a profound understanding of tumour hypoxia is of paramount importance. Therefore, the aim of this work was to investigate the relationship between tumour hypoxia and the expression of a number of hypoxia-induced proteins that play a role in tumour metabolism. The hypoxia marker pimonidazole was administered to dogs affected by spontaneous mammary carcinoma and compared with immunohistochemical staining for GLUT1 and 3, HK 2 and CA IX. A statistically significant correlation was found between pimonidazole staining and GLUT1-expression (R=0.607; p=0.001). These results indicate a strong interaction between tumour hypoxia and tumour metabolism by the induction of proteins essential to maintain a stable tumour microenvironment.
authors.affiliation: Department of Nuclear Medicine and Molecular Imaging, University Medical Center Groningen, University of Groningen, The Netherlands.
authors.forename: G
authors.initials: G
authors.lastname: Mees
authors.forename: C
authors.initials: C
authors.lastname: Vangestel
authors.forename: R
authors.initials: R
authors.lastname: Dierckx
authors.forename: S
authors.initials: S
authors.lastname: Loomans
authors.forename: N
authors.initials: N
authors.lastname: Van Damme
authors.forename: K
authors.initials: K
authors.lastname: Peremans
authors.forename: H
authors.initials: H
authors.lastname: De Rooster
authors.forename: B
authors.initials: B
authors.lastname: Van Goethem
authors.forename: P
authors.initials: P
authors.lastname: Pauwels
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
authors.forename: C
authors.initials: C
authors.lastname: Van de Wiele
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism* / Q000601:surgery; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D015674:Mammary Neoplasms, Animal / Q000378:metabolism* / Q000601:surgery; D009593:Nitroimidazoles; D010101:Oxygen Consumption; D011838:Radiation-Sensitizing Agents; D013194:Staining and Labeling
chemical_list: D051272:Glucose Transporter Type 1; D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; C033815:pimonidazole