title: OsDOG, a gibberellin-induced A20/AN1 zinc-finger protein, negatively regulates gibberellin-mediated cell elongation in rice.
abstract: The A20/AN1 zinc-finger proteins (ZFPs) play pivotal roles in animal immune responses and plant stress responses. From previous gibberellin (GA) microarray data and A20/AN1 ZFP family member association, we chose Oryza sativa dwarf rice with overexpression of gibberellin-induced gene (OsDOG) to examine its function in the GA pathway. OsDOG was induced by gibberellic acid (GA(3)) and repressed by the GA-synthesis inhibitor paclobutrazol. Different transgenic lines with constitutive expression of OsDOG showed dwarf phenotypes due to deficiency of cell elongation. Additional GA(1) and real-time PCR quantitative assay analyses confirmed that the decrease of GA(1) in the overexpression lines resulted from reduced expression of GA3ox2 and enhanced expression of GA2ox1 and GA2ox3. Adding exogenous GA rescued the constitutive expression phenotypes of the transgenic lines. OsDOG has a novel function in regulating GA homeostasis and in negative maintenance of plant cell elongation in rice.
authors.affiliation: Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Yaju
authors.initials: Y
authors.lastname: Liu
authors.forename: Yunyuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Qibin
authors.initials: Q
authors.lastname: Ma
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xue
authors.forename: Kang
authors.initials: K
authors.lastname: Chong
mesh_terms: D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000494:pharmacology*; D005966:Glucuronidase / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000502:physiology*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D014230:Triazoles / Q000494:pharmacology; D016335:Zinc Fingers / Q000187:drug effects / Q000235:genetics
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014230:Triazoles; C053370:paclobutrazol; D005966:Glucuronidase