title: [Expression profile of 60 lung cancer related genes in BEP2D cell and R15Hp35T-2 cell].
abstract: BACKGROUND
  To profile the expression patterns of 60 lung cancer related genes in human bronchial epithelial cell (BEP2D) and alpha-particle induced malignantly transformed cell (R15Hp35T-2).
  
  
  METHODS
  Sixty lung cancer related cDNAs were micro-arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from BEP2D cell and R15Hp35T-2 cell was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA.
  
  
  RESULTS
  Compared with the BEP2D cell, 27 genes up-regulated and 7 down-regulated in the R15Hp35T-2 cell. The expression abundance of most tumor suppressor genes were similar in the two kinds of cells, however, most oncogenes and growth factor genes were overexpressed in R15Hp35T-2 cell.
  
  
  CONCLUSIONS
  In malignantly transformed human bronchial epithelial cell model induced by alpha-particle, some oncogenes and growth factor genes may promote the malignant transformation together.
authors.affiliation: Department of Molecular Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R.China.
authors.forename: Baoxing
authors.initials: B
authors.lastname: Fan
authors.forename: Jingfen
authors.initials: J
authors.lastname: Sun
authors.forename: Kaitai
authors.initials: K
authors.lastname: Zhang
authors.forename: Dechang
authors.initials: D
authors.lastname: Wu