title: [Determination of four effective components from total flavonoids of Scutellaria barbata by high performance liquid chromatography].
abstract: To establish a HPLC method for simultaneous determination of 4 effective components from total flavonoids of Scutellaria barbata (FSB).
  
  
  
  The HPLC method was developed on an Agilent Zorbax C₁₈ column (4.6 mm × 250 mm, 5 μm). The mobile phase was composed of 1% HAc and CH₃OH:CH₃CN (80:20) with a linear gradient elution. The flow rate was 1.0 ml/min, and UV detection wave length was set at 280 nm. The column temperature was maintained at 30°C.
  
  
  
  The linear range of 4 effective components (scutellarin, isoscutellarein-8-O-glucuronide, isoscutellarein and luteolin) was 0.14-11.20 μg, 0.03-2.40 μg, 0.007-0.560 μg and 0.027-2.160 μg, respectively. The average recovery for 4 effective components was (101.9 ± 1.4)%, (103.5 ± 0.6)%, (98.1 ± 2.9)% and (100.5 ± 2.3)%, respectively. The contents of 4 flavonoids were determined, with scutellarin 7.3%-14.3%, isoscutellarein-8-O-glucuronide 2.4%-9.3%, isoscutellarein 0.3%-0.5%, and luteolin 0.2%-0.6%, respectively.
  
  
  
  The method can be used effectively to evaluate the quality of FSB.
authors.affiliation: Department of Chinese Materia Medica, Zhejiang University, Hangzhou 310058, China.
authors.forename: Lian-jun
authors.initials: LJ
authors.lastname: Luan
authors.forename: Ye-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Yong-jiang
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D047310:Apigenin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D047309:Flavones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D005965:Glucuronates / Q000032:analysis; D047311:Luteolin / Q000032:analysis; D031363:Scutellaria / Q000737:chemistry*
chemical_list: D047309:Flavones; D005419:Flavonoids; D005965:Glucuronates; C484876:scutellarin; C000722867:isoscutellarein; D047310:Apigenin; D047311:Luteolin