title: High levels of apolipoproteins found in the soluble fraction of avian cornea.
abstract: Water-soluble proteins in avian corneas were profiled by two-dimensional electrophoresis and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Comparative protein profiling of avian and mammalian corneas revealed five major protein spots specifically detected in avian species. These proteins were identified as apolipoproteins A1 and D by tandem mass spectrometry sequencing. This is the first report of the presence of apolipoproteins in avian cornea. These results could provide insight into the role of lipid metabolism in the avian-specific function of cornea.
authors.forename: Naoki
authors.initials: N
authors.lastname: Tsukahara
authors.forename: Yuri
authors.initials: Y
authors.lastname: Tani
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nihei
authors.forename: Yukihito
authors.initials: Y
authors.lastname: Kabuyama
authors.forename: Shoei
authors.initials: S
authors.lastname: Sugita
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis*; D053399:Apolipoproteins D / Q000032:analysis*; D002645:Chickens; D003315:Cornea / Q000737:chemistry*; D046368:Crows; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007525:Isoelectric Focusing; D050356:Lipid Metabolism; D008297:Male; D010446:Peptide Fragments; D011817:Rabbits; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D034421:Sus scrofa
chemical_list: D016632:Apolipoprotein A-I; D053399:Apolipoproteins D; D010446:Peptide Fragments