title: Long-term type 1 diabetes enhances in-stent restenosis after aortic stenting in diabetes-prone BB rats.
abstract: Type 1 diabetic patients have increased risk of developing in-stent restenosis following endovascular stenting. Underlying pathogenetic mechanisms are not fully understood partly due to the lack of a relevant animal model to study the effect(s) of long-term autoimmune diabetes on development of in-stent restenosis. We here describe the development of in-stent restenosis in long-term (~7 months) spontaneously diabetic and age-matched, thymectomized, nondiabetic Diabetes Prone BioBreeding (BBDP) rats (n = 6-7 in each group). Diabetes was suboptimally treated with insulin and was characterized by significant hyperglycaemia, polyuria, proteinuria, and increased HbA(1c) levels. Stented abdominal aortas were harvested 28 days after stenting. Computerized morphometric analysis revealed significantly increased neointima formation in long-term diabetic rats compared with nondiabetic controls. In conclusion, long-term autoimmune diabetes in BBDP rats enhances in-stent restenosis. This model can be used to study the underlying pathogenetic mechanisms of diabetes-enhanced in-stent restenosis as well as to test new therapeutic modalities.
authors.affiliation: Section of Immunology, Department of Cell Biology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands.
authors.forename: Geanina
authors.initials: G
authors.lastname: Onuta
authors.forename: Hendrik C
authors.initials: HC
authors.lastname: Groenewegen
authors.forename: Flip A
authors.initials: FA
authors.lastname: Klatter
authors.forename: Mark
authors.initials: M
authors.lastname: Walther Boer
authors.forename: Maaike
authors.initials: M
authors.lastname: Goris
authors.forename: Harry
authors.initials: H
authors.lastname: van Goor
authors.forename: Anton J M
authors.initials: AJ
authors.lastname: Roks
authors.forename: Jan
authors.initials: J
authors.lastname: Rozing
authors.forename: Bart J G L
authors.initials: BJ
authors.lastname: de Smet
authors.forename: Jan-Luuk
authors.initials: JL
authors.lastname: Hillebrands
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D001786:Blood Glucose / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D006083:Graft Occlusion, Vascular / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D007668:Kidney / Q000503:physiopathology; D011141:Polyuria / Q000503:physiopathology; D011507:Proteinuria / Q000503:physiopathology; D051381:Rats
chemical_list: D001786:Blood Glucose; D005947:Glucose; D006442:Glycated Hemoglobin