title: Effect of enzyme additions on methane production and lignin degradation of landfilled sample of municipal solid waste.
abstract: Operation of waste cells as landfill bioreactors with leachate recirculation is known to accelerate waste degradation and landfill gas generation. However, waste degradation rates in landfill bioreactors decrease with time, with the accumulation of difficult to degrade materials, such as lignin-rich waste. Although, potential exists to modify the leachate quality to promote further degradation of such waste, very little information is available in literature. The objective of this study was to determine the viability of augmenting leachate with enzymes to increase the rate of degradation of lignin-rich waste materials. Among the enzymes evaluated MnP enzyme showed the best performance in terms of methane yield and substrate (lignin) utilization. Methane production of 200 mL CH(4)/g VS was observed for the MnP amended reactor as compared to 5.7 mL CH(4)/g VS for the control reactor. The lignin reduction in the MnP amended reactor and control reactor was 68.4% and 6.2%, respectively.
authors.affiliation: Centre for Environmental Engineering Research and Education (CEERE), Schulich School of Engineering, University of Calgary, Calgary, AB, Canada, T2N 1N4.
authors.forename: P A
authors.initials: PA
authors.lastname: Jayasinghe
authors.forename: J P A
authors.initials: JP
authors.lastname: Hettiaratchi
authors.forename: A K
authors.initials: AK
authors.lastname: Mehrotra
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000416:Alberta; D019149:Bioreactors; D004798:Enzymes / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis*; D012037:Refuse Disposal / Q000379:methods*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D004798:Enzymes; D014874:Water Pollutants, Chemical; C036909:lignocellulose; D008031:Lignin; D006861:Hydrogen Peroxide; D008697:Methane