title: NF-Y affects histone acetylation and H2A.Z deposition in cell cycle promoters.
abstract: Histones post-translational modifications (PTMs) are crucial for transcriptional control, defining positive and negative chromatin territories. We previously described an extensive methylation-acetylation switch on cell cycle promoters using a single nucleosome ChIP assay. A key issue is how PTMs are locally positioned. We report an analysis on the role of the NF-Y CCAAT transcription factor on histone acetylation. Whereas H3K9 and H3K14 acetylation in core promoters is not influenced by NF-Y, H3K18ac, H3K36ac and H3K27ac are increased in the absence of NF-Y. Interestingly, NF-Y affects H2B acetylation in an opposite way: H2BK16ac is decreased and Lysine 120 acetylation, which counter-correlates with ubiquitination, increases dramatically upon NF-Y removal. KAT2A/KAT2B and subunits of the SAGA and ATAC complexes (SPT20 and ZZZ3) are differentially regulated. Finally, the deposition of H2A.Z, which maps around the TSS, is also NF-Y-dependent. In summary, NF-Y influences histone acetylation in different processes, including those involved in a methylation-acetylation switch and in the recruitment of histone variants.
authors.affiliation: Dipartimento di Scienze Biomolecolari e Biotecnologie, Università di Milano, Milano, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Gatta
authors.forename: Roberto
authors.initials: R
authors.lastname: Mantovani
mesh_terms: D000107:Acetylation; D023081:CCAAT-Binding Factor / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D056765:Cyclin B2 / Q000235:genetics / Q000378:metabolism; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D054875:Ubiquitination; D000099224:p300-CBP-Associated Factor
chemical_list: D023081:CCAAT-Binding Factor; D018797:Cell Cycle Proteins; D056765:Cyclin B2; D051548:Histone Acetyltransferases; D006657:Histones; D014157:Transcription Factors; C503758:CCNB2 protein, human; C502592:SUPT20H protein, human; C501179:KAT2A protein, human; D000099224:p300-CBP-Associated Factor