title: Impact of ω-5 gliadin on wheat-dependent exercise-induced anaphylaxis in mice.
abstract: The effects of ω-5 gliadin on wheat-dependent exercise-induced anaphylaxis (WDEIA) were investigated by using a mouse model. The gliadin fraction was prepared as a 70% ethanol-soluble solution, and ω-5 gliadin was purified by chromatography. Purified ω-5 gliadin was run on SDS-PAGE gel to reveal three bands with a molecular mass of 53-60 kDa and had the characteristic N-terminal sequence of ω-5 gliadin. The mice were sensitized to the gliadin fraction, and the anaphylactic response was assessed by measuring the body temperature and voluntary physical activity. An oral administration of ω-5 gliadin evoked a significant drop in both the body temperature and voluntary physical activity, similar to the effects of the whole gliadin fraction. ELISA and immunoblotting analyses revealed that the IgE expression from sensitized mice reacted most strongly to ω-5 gliadin. Taken together, these results indicate ω-5 gliadin to be a major allergen responsible for stimulating WDEIA in mice, with the characteristic potential for stimulating IgE production.
authors.affiliation: Department of Clinical Nutrition, Kawasaki University of Medical Welfare, Kurashiki, Okayama, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Nagano
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yano
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
authors.forename: Tatsuya M
authors.initials: TM
authors.lastname: Ikeda
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000707:Anaphylaxis / Q000097:blood / Q000209:etiology* / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D052179:Antigens, Plant; D005260:Female; D005903:Gliadin / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D051379:Mice; D010805:Physical Conditioning, Animal / Q000009:adverse effects*; D014908:Triticum
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C475180:omega-5 gliadin, wheat; D007073:Immunoglobulin E; D005903:Gliadin