title: Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica.
abstract: In this study, we describe the molecular characterization of the PmPEX14 gene encoding the peroxisomal membrane protein from the methylotrophic yeast Pichia methanolica. The pex14Δ strain of P. methanolica lost its ability to grow on methanol and oleate but grew normally on glucose. Disruption of the PmPEX14 caused a decrease in the activities of peroxisomal methanol-metabolizing enzymes and mislocalization of those proteins into the cytosol and vacuole. Taken together, these findings show that PmPex14p has an essential physiological role in methanol metabolism in P. methanolica.
authors.affiliation: Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099-2493, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Shotaro
authors.initials: S
authors.lastname: Ozawa
authors.forename: Shuki
authors.initials: S
authors.lastname: Fujimura
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Matsufuji
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakagawa
authors.forename: Noboru
authors.initials: N
authors.lastname: Tomizuka
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayakawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology*; D010843:Pichia / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D019301:Oleic Acid; D005947:Glucose; D000432:Methanol