title: Involvement of the UL24 protein in herpes simplex virus 1-induced dispersal of B23 and in nuclear egress.
abstract: UL24 of herpes simplex virus 1 (HSV-1) is widely conserved within the Herpesviridae family. Herein, we tested the hypothesis that UL24, which we have previously shown to induce the redistribution of nucleolin, also affects the localization of the nucleolar protein B23. We found that HSV-1-induced dispersal of B23 was dependent on UL24. The conserved N-terminal portion of UL24 was sufficient to induce the redistribution of B23 in transient transfection assays. Mutational analysis revealed that the endonuclease motif of UL24 was important for B23 dispersal in both transfected and infected cells. Nucleolar protein relocalization during HSV-1 infection was also observed in non-immortalized cells. Analysis of infected cells by electron microscopy revealed a decrease in the ratio of cytoplasmic versus nuclear viral particles in cells infected with a UL24-deficient strain compared to KOS-infected cells. Our results suggest that UL24 promotes nuclear egress of nucleocapsids during HSV-1 infection, possibly though effects on nucleoli.
authors.affiliation: INRS-Institut Armand-Frappier, Université du Québec, 531 boul. des Prairies, Laval, Québec H7V 1B7, Canada.
authors.forename: Maria H
authors.initials: MH
authors.lastname: Lymberopoulos
authors.forename: Amélie
authors.initials: A
authors.lastname: Bourget
authors.forename: Nawel
authors.initials: N
authors.lastname: Ben Abdeljelil
authors.forename: Angela
authors.initials: A
authors.lastname: Pearson
mesh_terms: D000818:Animals; D002460:Cell Line; D004252:DNA Mutational Analysis; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D009687:Nuclear Proteins; C086267:UL24 protein, Human herpesvirus 1; D014764:Viral Proteins; D000090243:Nucleophosmin