title: Rapid and quantitative assessment of cell quality, identity, and functionality for cell-based assays using real-time cellular analysis.
abstract: Strict quality control of cells is required for the standardization and interpretation of results in all areas of cell-based research, especially in drug discovery. Real-time cellular analysis using electrical impedance as a readout offers a rapid and highly reproducible method for quality control as it provides a quantitative measure of overall cell morphology and growth. In a case study, the authors demonstrate that samples of a single cell line obtained from several different labs show clear differences in their impedance profiles when compared with the corresponding standard cell line. A number of kinetic parameters were derived from the impedance profiles and used to quantify the differences among these cell lines. Our findings indicate that this methodology can detect cell line differences including mix-ups or contaminations, genetic alterations, and potential epigenetic changes occurring during passaging, all of which can occur in the time scale of a screening campaign. Finally, we provide evidence that these impedance profile differences can be predictive of different outcomes in cell-based functional assays for the effects of small molecules on otherwise seemingly identical cell lines.
authors.affiliation: ACEA Biosciences, Inc., San Diego, California 92121, USA. jirelan@aceabio.com
authors.forename: Jeffrey T
authors.initials: JT
authors.lastname: Irelan
authors.forename: Meng-Jou
authors.initials: MJ
authors.lastname: Wu
authors.forename: Jonathan
authors.initials: J
authors.lastname: Morgan
authors.forename: Ning
authors.initials: N
authors.lastname: Ke
authors.forename: Biao
authors.initials: B
authors.lastname: Xi
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.forename: Yama A
authors.initials: YA
authors.lastname: Abassi
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity; D001681:Biological Assay / Q000379:methods*; D045744:Cell Line, Tumor; D002477:Cells / Q000166:cytology / Q000187:drug effects; D003584:Cytological Techniques / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D007976:Leupeptins / Q000633:toxicity; D018895:Microsatellite Repeats / Q000235:genetics; D011786:Quality Control; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D007976:Leupeptins; D043562:Receptors, G-Protein-Coupled; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde