title: Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity.
abstract: We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong RD 130, Shanghai, 200237, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Bin-Cheng
authors.initials: BC
authors.lastname: Yin
authors.forename: Xian-Fei
authors.initials: XF
authors.lastname: Wang
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
mesh_terms: D015374:Biosensing Techniques; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006868:Hydrolysis; D010087:Oxides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010087:Oxides; D010455:Peptides; D006108:Graphite; D013917:Thrombin