title: Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors.
abstract: We previously reported that machine perfusion (MP) performed at 20°C enhanced the preservation of steatotic rat livers. Here, we tested whether rat livers retrieved 30 min after cardiac arrest (NHBDs) were better protected by MP at 20°C than with cold storage. We compared the recovery of livers from NHBDs with organs obtained from heart beating donors (HBDs) preserved by cold storage. MP technique: livers were perfused for 6h with UW-G modified at 20°C. Cold storage: livers were perfused in situ and preserved with UW solution at 4°C for 6h. Both MP and cold storage preserved livers were reperfused with Krebs-Heinselet buffer (2h at 37°C). AST and LDH release and mitochondrial glutamate dehydrogenase (GDH) levels were evaluated. Parameters assessed included: bile production and biliary enzymes; tissue ATP; reduced and oxidized glutathione (GSH/GSSG); protein-SH group concentration. Livers preserved by MP at 20°C showed significantly lower hepatic damage at the end of reperfusion compared with cold storage. GDH release was significantly reduced and bile production, ATP levels, GSH/GSSG and protein-SH groups were higher in livers preserved by MP at 20°C than with cold storage. The best preserved morphology and high glycogen content was obtained with livers submitted to MP at 20°C. Liver recovery using MP at 20°C was comparable to recovery with HBDs. MP at 20°C improves cell survival and gives a better-quality of preservation for livers obtained from NHBDs and may provide a new method for the successful utilization of marginal livers.
authors.affiliation: Department of Internal Medicine and Therapeutics, University of Pavia, Via Ferrata 9A, Pavia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferrigno
authors.forename: Vittoria
authors.initials: V
authors.lastname: Rizzo
authors.forename: Eleonora
authors.initials: E
authors.lastname: Boncompagni
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianchi
authors.forename: Enrico
authors.initials: E
authors.lastname: Gringeri
authors.forename: Daniele
authors.initials: D
authors.lastname: Neri
authors.forename: Plinio
authors.initials: P
authors.lastname: Richelmi
authors.forename: Isabel
authors.initials: I
authors.lastname: Freitas
authors.forename: Umberto
authors.initials: U
authors.lastname: Cillo
authors.forename: Mariapia
authors.initials: M
authors.lastname: Vairetti
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D003080:Cold Temperature; D005969:Glutamate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D019803:Glutathione Disulfide / Q000032:analysis / Q000378:metabolism; D006003:Glycogen / Q000032:analysis* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D017082:Portal Pressure; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism; D014019:Tissue Donors
chemical_list: D013438:Sulfhydryl Compounds; D000255:Adenosine Triphosphate; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D005978:Glutathione; D019803:Glutathione Disulfide