title: Molecular analysis of bacterial communities in uranium ores and surrounding soils from Banduhurang open cast uranium mine, India: A comparative study.
abstract: Bacterial community structure of heavy metal rich- uranium ores and surrounding soils was explored using 16S rRNA gene based clone library analysis and denaturing gradient gel electrophoresis (DGGE) to provide baseline microbial diversity data on autochthonous communities. Sequence analysis of major ribotypes and/or DGGE bands revealed Proteobacteria and Acidobacteria as the two most frequently present bacterial phyla across the samples, although relative abundance of each phyla and identity of their members at lower taxonomic level showed marked difference. Gammaproteobacteria (Pseudomonas and Escherichia) was most abundant in U-ore samples along with the lineages of β-Proteobacteria (Burkholderia and Janthinobacterium), α-Proteobacteria (Brevundimonas), Bacteroidetes (Spingobacterium), Firmicutes (Peptoniphilus), Actinobacteria (Corynebacterium), uncultured -Acidobacteria, -Chloroflexi and -Cyanobacterium. In contrast to this soil communities were represented by mixed populations predominated by uncultured Acidobacteria along with Gammaproteobacteria (Succinivibrio, Cellovibrio and Legionella), β-Proteobacteria (Rhodocyclus), α-Proteobacteria (Methylocystis and Phenylobacterium), δ-Proteobacteria, unclassified bacteria, uncultured Bacteroidetes, Firmicutes (Bacillus), Cyanobacteria (Scytonema), Actinobacteria (Actinomadura) and candidate division TM7. Principle Component Analyis (PCA) of geochemical data and UPGMA cluster analysis of DGGE profiles were in close agreement showing characteristic relatedness of samples obtained from either ores or soils. Our analysis indicated that soils surrounding the ore deposit bear specific geochemical as well as microbiologial characteristics distinct from the ore deposit and therefore these data obtained at the onset of mining could serve as a baseline of information to gauge the subsequent environmnetal impact of U-mining.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, India.
authors.forename: Ekramul
authors.initials: E
authors.lastname: Islam
authors.forename: Paltu K
authors.initials: PK
authors.lastname: Dhal
authors.forename: Sufia K
authors.initials: SK
authors.lastname: Kazy
authors.forename: Pinaki
authors.initials: P
authors.lastname: Sar
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D044822:Biodiversity; D016000:Cluster Analysis; D019295:Computational Biology; D058645:Denaturing Gradient Gel Electrophoresis; D007194:India; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D025341:Principal Component Analysis; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D017974:Uranium Compounds / Q000032:analysis*
chemical_list: D012337:RNA, Ribosomal, 18S; D012987:Soil; D017974:Uranium Compounds