title: The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis.
abstract: The YvcK protein was previously shown to be dispensable when B. subtilis cells are grown on glycolytic carbon sources but essential for growth and normal shape on gluconeogenic carbon sources. Here, we report that YvcK is localized as a helical-like pattern in the cell. This localization seems independent of the actin-like protein, MreB. A YvcK overproduction restores a normal morphology in an mreB mutant strain when bacteria are grown on PAB medium. Reciprocally, an additional copy of mreB restores a normal growth and morphology in a yvcK mutant strain when bacteria are grown on a gluconeogenic carbon source like gluconate. Furthermore, as already observed for the mreB mutant, the deletion of the gene encoding the penicillin-binding protein PBP1 restores growth and normal shape of a yvcK mutant on gluconeogenic carbon sources. The PBP1 is delocalized in an mreB mutant grown in the absence of magnesium and in a yvcK mutant grown on gluconate medium. Interestingly, its proper localization can be rescued by YvcK overproduction. Therefore, in gluconeogenic growth conditions, YvcK is required for the correct localization of PBP1 and hence for displaying a normal rod shape.
authors.affiliation: Laboratoire de Chimie Bactérienne, UPR 9043 Service d'Imagerie Cellulaire, IFR 88, CNRS, Université de la Méditerranée, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Foulquier
authors.forename: Frédérique
authors.initials: F
authors.lastname: Pompeo
authors.forename: Alain
authors.initials: A
authors.lastname: Bernadac
authors.forename: Leon
authors.initials: L
authors.lastname: Espinosa
authors.forename: Anne
authors.initials: A
authors.lastname: Galinier
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D005943:Gluconeogenesis; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins