title: Significant improvement of Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase production using response surface methodology.
abstract: The medium optimization for the production of the Geobacillus thermoleovorans CCR11 thermoalkalophilic lipase was carried out in shake flask cultures using safflower high oleic oil. In the first step of optimization, a two level fractional factorial design allowed the identification of the concentration of nutrient broth and temperature as the main variables significantly affecting lipase production (P<0.05). In a second step, a D-optimal design was applied to determine the variables optimal values, defined as those yielding maximal lipase production in shaken flasks, thus demonstrating that the optimal concentration of nutrient broth was 3.8 g/l and the optimal culture temperature was 39.5°C. The model was experimentally validated, yielding a lipase production of 2283.70 ± 118.36 U/mL which represents a 6.7-fold increase in comparison to the non-optimized medium.
authors.affiliation: Unidad de Investigación y Desarrollo en Alimentos, Instituto Tecnológico de Veracruz, M. A. de Quevedo 2779, C.P. 91897 Veracruz, Ver, Mexico.
authors.forename: María Guadalupe
authors.initials: MG
authors.lastname: Sánchez-Otero
authors.forename: Irving Israel
authors.initials: II
authors.lastname: Ruiz-López
authors.forename: David Eduardo
authors.initials: DE
authors.lastname: Avila-Nieto
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Oliart-Ros
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D056506:Geobacillus / Q000201:enzymology* / Q000254:growth & development*; D008049:Lipase / Q000096:biosynthesis*; D008954:Models, Biological; D012450:Safflower Oil / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D012450:Safflower Oil; D008049:Lipase