title: [IL-1β can promote proliferation and migration of Hepa1-6 cells and impair IFNα-induced cell growth inhibition in vitro].
abstract: OBJECTIVE
  To analyze the effect of recombinant IL-1β on proliferation, migration, and the effect on IFNα induced cell growth inhibtion.
  
  
  METHODS
  The vector pLIVE-mIL-1β was transfected into Hepa1-6 cells mediated by transIT-LT1. Gene expression level of IL-1β was analyzed by RT-PCR and Sandwich ELISA. Cell migration was assessed using wound healing assay.
  
  
  RESULTS
  IL-1β significantly stimulated proliferation and migration of Hepa1-6 cells. However, expression of IL-1β significantly down-regulated growth inhibition inducecd by IFNα.
  
  
  CONCLUSIONS
  The recombinant vector could stably express IL-1β and promote in vitro proliferation, migration, and impair IFNα-induced cell growth inhibition.
authors.affiliation: Department of Immunology, Weifang Medical College, Key Laboratory of Immunology in Universities of Shandong, Weifang 261053, China.
authors.forename: Zhi-Juan
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Liang
authors.forename: Huan-Qin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Li
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Xiao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D016898:Interferon-alpha / Q000494:pharmacology; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D016898:Interferon-alpha; D053583:Interleukin-1beta