title: B2 RNA represses TFIIH phosphorylation of RNA polymerase II.
abstract: Mouse B2 RNA represses RNA polymerase II (Pol II) transcription during the cellular heat shock response. B2 RNA binds Pol II, enters complexes at promoters, and keeps the polymerase from engaging the DNA. Here we show that phosphorylation of Ser5 residues in the Pol II carboxy terminal domain (CTD) decreases after heat shock at the promoter of the repressed actin gene in mouse cells, despite the continued presence of Cdk7 and cyclin H. Biochemical assays revealed that B2 RNA, when present with Pol II in promoter-bound complexes, specifically represses CTD phosphorylation by TFIIH.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado, Boulder, USA.
authors.forename: Petro
authors.initials: P
authors.lastname: Yakovchuk
authors.forename: James A
authors.initials: JA
authors.lastname: Goodrich
authors.forename: Jennifer F
authors.initials: JF
authors.lastname: Kugel
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D047369:Chromatin Immunoprecipitation; D056748:Cyclin H / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D006358:Hot Temperature; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D051758:Transcription Factor TFIIH / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D056748:Cyclin H; D018844:Cyclin-Dependent Kinases; D011494:Protein Kinases; D012319:RNA Polymerase II; D022661:RNA, Untranslated; D012694:Serine; D051758:Transcription Factor TFIIH; C060137:carboxy-terminal domain kinase; C090586:Cdk7 protein, mouse; C522173:Rpb1 protein, mouse