title: Scanning electron microscopy of fibrin networks in rheumatoid arthritis: a qualitative analysis.
abstract: Rheumatoid arthritis is a chronic inflammatory condition that affects mainly synovial joints and has an impact on approximately 1% of the Western population. The coagulation process is altered in this condition, and this is frequently complicated by thrombocytosis. Changes in fibrin morphology have been linked with inflammation, and this, in turn, plays an important role in thrombosis. Changes in the fibrin fiber formation cause the alterations observed in thrombus morphology. In the current study, the ultrastructure of platelets and fibrin networks was investigated to determine whether any morphological changes are present in these structures in patients suffering from rheumatoid arthritis. Six patients diagnosed with rheumatoid arthritis took part in this study, and their clot morphology was compared to that of control subjects. Citrated blood with and without the addition of thrombin was used. Results indicated that the fibrin networks in the arthritis patients formed thick, matted layers. This matted appearance is due to a changed ultrastructure of the minor, thin fibers. Also, in these patients, spontaneous networks were created without the addition of thrombin, which indicates an abnormal hemostatic protein functioning, and the latter is expressed as visible changes in ultrastructure.
authors.affiliation: Department of Anatomy, School of Medicine, Faculty of Health Sciences, University of Pretoria, BMW Building, PO Box 2034, Pretoria, 0001, South Africa. resia.pretorius@up.ac.za
authors.forename: Etheresia
authors.initials: E
authors.lastname: Pretorius
authors.forename: Hester Magdalena
authors.initials: HM
authors.lastname: Oberholzer
authors.forename: Wendy Jeannette
authors.initials: WJ
authors.lastname: van der Spuy
authors.forename: Albe Carina
authors.initials: AC
authors.lastname: Swanepoel
authors.forename: Prashilla
authors.initials: P
authors.lastname: Soma
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology / Q000473:pathology*; D001777:Blood Coagulation; D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure*; D016022:Case-Control Studies; D005260:Female; D005337:Fibrin / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D013019:South Africa; D013917:Thrombin / Q000378:metabolism
chemical_list: D005337:Fibrin; D013917:Thrombin