title: Effects of urinary trypsin inhibitor on lipopolysaccharide-induced acute lung injury in rabbits.
abstract: This study was undertaken to clarify the effects of urinary trypsin inhibitor (UTI) on lipopolysaccharide (LPS)-induced acute lung injury. Rabbits were randomly assigned to one of seven groups: saline only, UTI, LPS, pre- or post-UTI-high (infusion of UTI of 25,000 U/kg followed by 25,000 U/kg over 2 h), pre- or post-UTI-low (infusion of UTI of 2,500 U/kg followed by 2,500 U/kg over 2 h). UTI was administered 30 min before (pre-groups) or 15 min after (post-groups) LPS administration. Rabbits were mechanically ventilated with 40% oxygen for 6 h. LPS decreased peripheral blood leukocyte counts and increased wet/dry weight ratio of lung, lung injury score, neutrophil infiltration in lung, and IL-8 production in systemic blood and bronchoalveolar lavage fluid (BALF). Rabbits treated by UTI were protected from LPS-induced lung injury, as determined by wet/dry weight ratio, neutrophil infiltration in lung, lung injury score, and IL-8 in BALF levels. UTI attenuated LPS-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in sepsis-related lung injury.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 5 Hak dong, Gwangju, 501 746, South Korea.
authors.forename: Hong-Beom
authors.initials: HB
authors.lastname: Bae
authors.forename: Cheol-Won
authors.initials: CW
authors.lastname: Jeong
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kwak
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D006023:Glycoproteins / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects; D011817:Rabbits; D011897:Random Allocation
chemical_list: D006023:Glycoproteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; C028665:urinastatin