title: [The effect of Crocin against hypoxia damage of myocardial cell and its mechanism].
abstract: OBJECTIVE
  To investigate the protective effect of Crocin against hypoxia damage of cardiac myocytes of neonatal rats and the regulation of HIF-1 and prolyhydroxylase (PHDs).
  
  
  METHODS
  A model of CoCl2 simulated hypoxia damage was established in primary cultural myocardial cell. Expression levels of HIF-1alpha, VEGF, iNOS, as well as PHD1, 2, 3 protein in myocardial cells were detected by Western blot.
  
  
  RESULTS
  Compared with CoCl2 group, the viability of myocardial cell was significantly increased after treated 24 h at 10(-5)mol/L Crocin (P < 0.01), HIF-1alpha, VEGF and iNOS were expressed higher than those in Crocin + CoCl2 group (P < 0.01), the expression of PHD2 was significantly increased (P < 0.01), while the expression of PHD3 was remarkably reduced in Crocin + CoCl2 Group (P < 0.01).
  
  
  CONCLUSIONS
  Crocin has better protective effect on hypoxic damage of myocardial cell. The mechanisms of protective effect of Crocin may be related to the activation of HIF-1-mediated pathway of the hypoxia response. PHDs may be involved in the pathophysiology regulated process of myocardial cells.
authors.affiliation: Institute of Navigation Medicine, Nantong University, Nantong 226001, China. wy@ntu.edu.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Wu
authors.forename: Rui-Rong
authors.initials: RR
authors.lastname: Pan
authors.forename: Peng
authors.initials: P
authors.lastname: Geng
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000494:pharmacology*; D015687:Cell Hypoxia / Q000187:drug effects; D002478:Cells, Cultured; D018398:Homeodomain Proteins / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C497444:Hif1a protein, rat; D018398:Homeodomain Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C102937:Uncx protein, rat; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D002338:Carotenoids; C029036:crocin; D011393:Procollagen-Proline Dioxygenase; C580731:Egln1 protein, rat; C087100:Egln3 protein, rat; D064799:Hypoxia-Inducible Factor-Proline Dioxygenases; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat