title: Application of LC-MS/MS method for the in vivo metabolite determination of oleuropein after intravenous administration to rat.
abstract: A highly sensitive, specific and simple LC-MS/MS method was developed to investigate in vivo bio-transformation of oleuropein in rat. Rat urine samples collected after the intravenous administrations were determined using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative-ion mode. The assay procedure involves a simple liquid-liquid extraction of parent oleuropein and the metabolite from rat urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.80 mL/min on an RP-C(18) column with a total run time of 30 min. This method has been successfully applied to simultaneous determination of oleuropein and its metabolite in rat urine. Oxygenation was found to be the major metabolic pathway of the oleuropein in rat after intravenous administration.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.forename: Tianxiu
authors.initials: T
authors.lastname: Qian
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.forename: Xianen
authors.initials: X
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Shuangying
authors.initials: S
authors.lastname: Gui
mesh_terms: D000085:Acetates; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005561:Formates; D007275:Injections, Intravenous; D057888:Iridoid Glucosides; D039823:Iridoids; D059625:Liquid-Liquid Extraction; D008297:Male; D000432:Methanol; D011714:Pyrans / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000085:Acetates; D005561:Formates; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C030544:formic acid; C002769:oleuropein; C007650:ethyl acetate; D000432:Methanol