title: Interaction of plum pox virus with specific colloidal gold-labeled antibodies and development of immunochromatographic assay of the virus.
abstract: Two monoclonal antibodies (mABs) raised against plum pox virus (PPV) were shown to recognize its D, M, and C strains. Conjugates of the antibodies with colloidal gold (CG) nanoparticles averaging 26 nm in diameter were synthesized. The binding constants of PPV with both the native and conjugated mABs were determined using a Biacore X device. The complexes between the CG-mAB conjugates and plum pox virions were examined by means of transmission electron and atomic force microscopy. Using the conjugates with optimal component ratio, an express immunochromatographic assay of PPV was developed with a detection limit of 3 ng/ml and duration of 10 min. The assay was tested for PPV detection in samples of stone fruit tree leaves and demonstrated a good compatibility with the data obtained by "sandwich"-ELISA. The developed assay can be used in the field and applied for monitoring viral infection and for quarantine purposes.
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, Russia. nbyzova@inbi.ras.ru
authors.forename: N A
authors.initials: NA
authors.lastname: Byzova
authors.forename: I V
authors.initials: IV
authors.lastname: Safenkova
authors.forename: S N
authors.initials: SN
authors.lastname: Chirkov
authors.forename: V G
authors.initials: VG
authors.lastname: Avdienko
authors.forename: A N
authors.initials: AN
authors.lastname: Guseva
authors.forename: I V
authors.initials: IV
authors.lastname: Mitrofanova
authors.forename: A V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: B B
authors.initials: BB
authors.lastname: Dzantiev
authors.forename: J G
authors.initials: JG
authors.lastname: Atabekov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D005427:Flocculation; D017979:Gold Colloid; D007150:Immunohistochemistry; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles; D010316:Particle Size; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D017801:Plum Pox Virus / Q000276:immunology; D027861:Prunus / Q000821:virology; D011934:Reagent Strips; D014773:Virology / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D011934:Reagent Strips