title: Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum.
abstract: The alcohol dehydrogenase gene adhA in Corynebacterium glutamicum is subject to a complex carbon source-dependent regulation mediated by RamA, RamB and GlxR. In this study we identified SucR as the fourth transcriptional regulator involved in expression control of the adhA gene. SucR specifically binds to the adhA promoter and acts as transcriptional repressor independent of the carbon source used. Furthermore, we found that SucR negatively controls the expression of its own gene. This negative autoregulation is mediated by binding of SucR to at least one of four identified binding sites located in the promoter region of sucR. EMSA experiments and subsequent sequence analysis led to the identification of the SucR consensus binding sequence YYAACAWMAW. This binding motif is different from the binding site (ACTCTAGGGG) recently described for SucR in the promoter region of the sucCD operon. However, we were not able to detect a specific interaction of purified SucR protein with this motif present in the sucCD promoter region.
authors.affiliation: Institute of Microbiology and Biotechnology, University of Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Auchter
authors.forename: Tanja
authors.initials: T
authors.lastname: Laslo
authors.forename: Constanze
authors.initials: C
authors.lastname: Fleischer
authors.forename: Laura
authors.initials: L
authors.lastname: Schiller
authors.forename: Annette
authors.initials: A
authors.lastname: Arndt
authors.forename: Lars
authors.initials: L
authors.lastname: Gaigalat
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Bernhard J
authors.initials: BJ
authors.lastname: Eikmanns
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D005798:Genes, Bacterial / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000426:Alcohol Dehydrogenase