title: The Caenorhabditis elegans JIP3 protein UNC-16 functions as an adaptor to link kinesin-1 with cytoplasmic dynein.
abstract: Kinesin-1 is a microtubule plus-end-directed motor that transports various cargos along the axon. Previous studies have elucidated the physical and genetic interactions between kinesin-1 and cytoplasmic dynein, a microtubule minus-end-directed motor, in neuronal cells. However, the physiological importance of kinesin-1 in the dynein-dependent retrograde transport of cargo molecules remains obscure. Here, we show that Caenorhabditis elegans kinesin-1 forms a complex with dynein via its interaction with UNC-16, which binds to the dynein light intermediate (DLI) chain. Both kinesin-1 and UNC-16 are required for localization of DLI-1 at the plus ends of nerve process microtubules. In addition, retrograde transport of APL-1 depends on kinesin-1, UNC-16, and dynein. These results suggest that kinesin-1 mediates the anterograde transport of dynein using UNC-16 as a scaffold and that dynein in turn mediates the retrograde transport of cargo molecules in vivo. Thus, UNC-16 functions as an adaptor for kinesin-1-mediated transport of dynein.
authors.affiliation: Department of Molecular Biology, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Arimoto
authors.forename: Sandhya P
authors.initials: SP
authors.lastname: Koushika
authors.forename: Bikash C
authors.initials: BC
authors.lastname: Choudhary
authors.forename: Chris
authors.initials: C
authors.lastname: Li
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Hisamoto
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D001692:Biological Transport; D019556:COS Cells; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002522:Chlorocebus aethiops; D056445:Cytoplasmic Dyneins / Q000235:genetics / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D047468:Immunoprecipitation / Q000379:methods; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008465:Mechanoreceptors / Q000166:cytology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008845:Microinjections / Q000379:methods; D017729:Presynaptic Terminals / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: C084891:APL-1 protein, C elegans; D048868:Adaptor Proteins, Signal Transducing; D029742:Caenorhabditis elegans Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; C445161:unc-16 protein, C elegans; C444431:Dli-1 protein, C elegans; D056445:Cytoplasmic Dyneins; D004398:Dyneins; D016547:Kinesins