title: [Study on HPLC fingerprinting and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice].
abstract: OBJECTIVE
  To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.
  
  
  METHODS
  HPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.
  
  
  RESULTS
  Baifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.
  
  
  CONCLUSIONS
  This method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.
authors.affiliation: Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
authors.forename: Dongan
authors.initials: D
authors.lastname: Chen
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yi
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Huang
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Li
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000592:standards; D008516:Medicine, Chinese Traditional / Q000592:standards; D011786:Quality Control
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal