title: Development and validation of a new HPLC-UV method for the simultaneous determination of triclabendazole and ivermectin B1a in a pharmaceutical formulation.
abstract: A rapid, simple, and sensitive RP-HPLC analytical method was developed for the simultaneous determination of triclabendazole and ivermectin in combination using a C18 RP column. The mobile phase was acetonitrile-methanol-water-acetic acid (56 + 36 + 7.5 + 0.5, v/v/v/v) at a pH of 4.35 and flow rate of 1.0 mL/min. A 245 nm UV detection wavelength was used. Complete validation, including linearity, accuracy, recovery, LOD, LOQ, precision, robustness, stability, and peak purity, was performed. The calibration curve was linear over the range 50.09-150.26 microg/mL for triclabendazole with r = 0.9999 and 27.01-81.02 microg/mL for ivermectin with r = 0.9999. Calculated LOD and LOQ for triclabendazole were 0.03 and 0.08 microg/mL, respectively, and for ivermectin 0.07 and 0.20 microg/mL, respectively. The intraday precision obtained was 98.71% with RSD of 0.87% for triclabendazole and 100.79% with RSD 0.73% for ivermectin. The interday precision obtained was 99.51% with RSD of 0.35% for triclabendazole and 100.55% with RSD of 0.59% for ivermectin. Robustness was also studied, and there was no significant variation of the system suitability of the analytical method with small changes in experimental parameters.
authors.affiliation: Al-Ahliyyah Amman University, Faculty of Pharmacy, Department of Medical Science, PO Box 113, Al-Sarou 19928, Jordan.
authors.forename: Maher
authors.initials: M
authors.lastname: Shurbaji
authors.forename: Mohamad H
authors.initials: MH
authors.lastname: Abu Al Rub
authors.forename: Munib M
authors.initials: MM
authors.lastname: Saket
authors.forename: Ali M
authors.initials: AM
authors.lastname: Qaisi
authors.forename: Maher L
authors.initials: ML
authors.lastname: Salim
authors.forename: Eyad S M
authors.initials: ES
authors.lastname: Abu-Nameh
mesh_terms: D000871:Anthelmintics / Q000032:analysis*; D001562:Benzimidazoles / Q000032:analysis*; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004355:Drug Stability; D007202:Indicators and Reagents; D007559:Ivermectin / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D000077682:Triclabendazole
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D004338:Drug Combinations; D007202:Indicators and Reagents; D012996:Solutions; D000077682:Triclabendazole; D007559:Ivermectin