title: Evaluation antimicrobial and antiadhesive properties of the biosurfactant Lunasan produced by Candida sphaerica UCP 0995.
abstract: Different groups of biosurfactants exhibit diverse properties and display a variety of physiological functions in producer microorganisms; these include enhancing the solubility of hydrophobic/water-insoluble compound, heave metal binding, bacterial pathogenesis, cell adhesion and aggregation, quorum sensing and biofilm formation. Candida sphaerica was grown in a low cost medium, consisting of distilled water supplemented with 9% refinery residue of soybean oil and 9% corn steep liquor, for 144 h at 28°C and 150 rpm. The cell-free supernatant obtained at the end of the experiments was submitted to extraction, and afterward the biosurfactant was isolated using methanol with a yield of 9 g l(-1). The critical micelle concentration of the biosurfactant was found to be 0.25 mg ml(-1) with a surface tension of 25 mN m(-1). Several concentrations of the biosurfactant (0.625-10 mg ml(-1)) were used to evaluate its antimicrobial and antiadhesive activities against a variety of microorganisms. The biosurfactant showed antimicrobial activity against Streptococcus oralis (68%), Candida albicans (57%), and Staphylococcus epidermidis(57.6%) for the highest concentration tested. Furthermore, the biosurfactant at a concentration of 10 mg ml(-1) inhibited the adhesion between 80 and 92% of Pseudomonas aeruginosa, Streptococcus agalactiae, Streptococcus sanguis12. Inhibition of adhesion with percentages near 100% occurred for the higher concentrations of biosurfactant used. Results gathered in this study point to a potential use of the biosurfactant in biomedical applications.
authors.affiliation: Universidade Federal de Pernambuco, Ciências Biológicas, Recife, PE, Brazil.
authors.forename: Juliana M
authors.initials: JM
authors.lastname: Luna
authors.forename: Raquel D
authors.initials: RD
authors.lastname: Rufino
authors.forename: Leonie A
authors.initials: LA
authors.lastname: Sarubbo
authors.forename: Lígia R M
authors.initials: LR
authors.lastname: Rodrigues
authors.forename: José A C
authors.initials: JA
authors.lastname: Teixeira
authors.forename: Galba M
authors.initials: GM
authors.lastname: de Campos-Takaki
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002175:Candida / Q000737:chemistry / Q000378:metabolism*; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D013291:Streptococcus / Q000187:drug effects / Q000502:physiology; D013501:Surface-Active Agents / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D013501:Surface-Active Agents