title: Presence of task-1 channel in the laryngeal mucosa in the newborn lamb.
abstract: Nearly 40 potassium channels have been described in respiratory epithelial cells. Of these are found several members of the 4-transmembrane domain, 2-pore K(+) channel family (K2P family), namely Twik-1 and -2, Trek-1 and -2, Task-2, -3, and -4, Thik-1, and KCNK7. The aim of this study was to verify whether the Twik-related acid-sensitive K(+) channel, subtype 1 (Task-1) (also known as KCNK3), is present in the laryngeal mucosa in the newborn lamb. Through the use of immunohistochemistry and nested polymerase chain reaction (PCR) amplification, results indicate that Task-1 protein and mRNA are present in the laryngeal mucosa, in both the ciliated, pseudostratified columnar (respiratory) epithelium and the nonkeratinized, stratified squamous epithelium. The complete ovine Task-1 protein sequence showed high homology levels with previously reported mouse, bovine, and human Task-1 sequences. This includes a complete homology for the C-terminal amino acid sequence, which is mandatory for protein trafficking to the cell membrane. These results represent the first demonstration that Task-1, a pH-sensitive channel responsible for setting membrane potential, is present in the laryngeal mucosa of a newborn mammal.
authors.affiliation: Neonatal Respiratory Research Unit, Department of Pediatrics, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
authors.forename: Véronique
authors.initials: V
authors.lastname: Bournival
authors.forename: Roxane
authors.initials: R
authors.lastname: Desjardins
authors.forename: Shirley
authors.initials: S
authors.lastname: Campbell
authors.forename: Claude
authors.initials: C
authors.lastname: Roberge
authors.forename: Alexandre
authors.initials: A
authors.lastname: Doueik
authors.forename: Louis
authors.initials: L
authors.lastname: Gendron
authors.forename: Marcel Daniel
authors.initials: MD
authors.lastname: Payet
authors.forename: Nicole
authors.initials: N
authors.lastname: Gallo-Payet
authors.forename: Robert
authors.initials: R
authors.lastname: Day
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Praud
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D007820:Laryngeal Mucosa / Q000737:chemistry*; D008564:Membrane Potentials; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D024683:Potassium Channels, Tandem Pore Domain / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012756:Sheep
chemical_list: D009419:Nerve Tissue Proteins; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger; C420132:potassium channel subfamily K member 3