title: Phosphatidylglucoside: a novel marker for adult neural stem cells.
abstract: We investigated the expression of a novel glycophospholipid, phosphatidylglucoside (PtdGlc), in adult mouse brains. Immunohistochemical analysis with DIM21 antibody, a monoclonal anti-PtdGlc antibody, revealed robust PtdGlc staining in the two primary neurogenic regions of the adult rodent brain, the subventricular zone (SVZ) lining the lateral ventricle and the subgranular zone of the dentate gyrus. Intriguingly, the staining pattern of PtdGlc appeared to overlap that of glial fibrillary acidic protein, an adult neural stem cell marker in these regions. Further immunohistochemical analysis revealed that PtdGlc expression on the cell membranes of adult SVZ neural stem cells significantly overlapped with other proposed adult neural stem cell markers. Moreover, PtdGlc(+) cells isolated from adult mouse SVZs by fluorescence-activated cell sorting with anti-PtdGlc antibody efficiently generated neurospheres in cell culture. These cells differentiated into neurons, astrocytes, and oligodendrocytes in vitro, directly demonstrating that PtdGlc-expressing cells possessed multipotency. Our data suggest that PtdGlc could be a useful adult stem cell marker.
authors.affiliation: Laboratory for Molecular Membrane Neuroscience, Brain Science Institute, RIKEN, Wako-shi, Saitama, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kaneko
authors.forename: Masami O
authors.initials: MO
authors.lastname: Kinoshita
authors.forename: Takeo
authors.initials: T
authors.lastname: Machida
authors.forename: Yoko
authors.initials: Y
authors.lastname: Shinoda
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Nagatsuka
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hirabayashi
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D053687:Adult Stem Cells / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology* / Q000254:growth & development; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005434:Flow Cytometry; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D020404:Glycerophospholipids / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D020404:Glycerophospholipids; D014404:Tubulin; C462941:beta3 tubulin, mouse; C429823:phosphatidylglucose; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D001973:Bromodeoxyuridine