title: Bovine Serum Albumin protofibril-like aggregates formation: solo but not simple mechanism.
abstract: We report an experimental study on the model protein Bovine Serum Albumin (BSA), with the aim of elucidating the mechanisms by which a fully folded globular protein undergoes different aggregation pathways leading to the formation of amyloid fibrils or amorphous aggregates. We observe thermally induced formation of fibrillar structures at pH far from the protein isoelectric point. The increase of electrostatic repulsion results in protein destabilization and in modifications of inter and intra-molecular interactions leading to the growth of fibril-like aggregates stabilized by inter-molecular-β sheets. The aggregation kinetics is studied by means of fluorescence techniques, light scattering, Circular Dichroism (CD), infrared spectroscopy (FTIR) and Atomic Force Microscopy (AFM). Changes in protein secondary structures turn out to be the driving mechanism of the observed aggregation and they progress in parallel with the growth of Thioflavin T emission intensity and scattering signal. This concurrent behavior suggests a mutual stabilization of elongated protofibril-like structures and of protein conformational and structural changes, which lead to a more rigid and ordered structures. Our results give new insights on BSA self-assembly process in alkaline conditions clearly providing new pieces of evidences of the interplay of several and interconnected mechanisms occurring on different time and length scales.
authors.affiliation: Dipartimento di Scienze Fisiche ed Astronomiche, University of Palermo, Via Archirafi 36, 90123 Palermo, Italy. valeria.vetri@fisica.unipa.it
authors.forename: Valeria
authors.initials: V
authors.lastname: Vetri
authors.forename: Michele
authors.initials: M
authors.lastname: D'Amico
authors.forename: Vito
authors.initials: V
authors.lastname: Foderà
authors.forename: Maurizio
authors.initials: M
authors.lastname: Leone
authors.forename: Andrea
authors.initials: A
authors.lastname: Ponzoni
authors.forename: Giorgio
authors.initials: G
authors.lastname: Sberveglieri
authors.forename: Valeria
authors.initials: V
authors.lastname: Militello
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011487:Protein Conformation; D055503:Protein Multimerization; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D055672:Static Electricity; D013696:Temperature; D013816:Thermodynamics; D013844:Thiazoles / Q000378:metabolism; D014364:Tryptophan
chemical_list: D052160:Benzothiazoles; D013844:Thiazoles; C009462:thioflavin T; D012710:Serum Albumin, Bovine; D014364:Tryptophan