title: The tricarboxylic acid cycle in L₃ Teladorsagia circumcincta: metabolism of acetyl CoA to succinyl CoA.
abstract: Nematodes, like other species, derive much of the energy for cellular processes from mitochondrial pathways including the TCA cycle. Previously, we have shown L₃ Teladorsagia circumcincta consume oxygen and so may utilise a full TCA cycle for aerobic energy metabolism. We have assessed the relative activity levels and substrate affinities of citrate synthase, aconitase, isocitrate dehydrogenase (both NAD+ and NADP+ specific) and α-ketoglutarate dehydrogenase in homogenates of L₃ T. circumcincta. All of these enzymes were present in homogenates. Compared with citrate synthase, low levels of enzyme activity and low catalytic efficiency was observed for NAD+ isocitrate dehydrogenase and especially α-ketoglutarate dehydrogenase. Therefore, it is likely that the activity of these to enzymes regulate overall metabolite flow through the TCA cycle, especially when [NAD+] limits enzyme activity. Of the enzymes tested, only citrate synthase had substrate affinities which were markedly different from values obtained from mammalian species. Overall, the results are consistent with the suggestion that a full TCA cycle exists withinL₃ T. circumcincta. While there may subtle variations in enzyme properties, particularly for citrate synthase, the control points for the TCA cycle inL₃ T. circumcincta are probably similar to those in the tissues of their host species.
authors.affiliation: Institute of Food Nutrition and Human Health, Massey University, Palmerston North 4442, New Zealand. D.C.Simcock@massey.ac.nz
authors.forename: D C
authors.initials: DC
authors.lastname: Simcock
authors.forename: L R
authors.initials: LR
authors.lastname: Walker
authors.forename: K C
authors.initials: KC
authors.lastname: Pedley
authors.forename: H V
authors.initials: HV
authors.lastname: Simpson
authors.forename: S
authors.initials: S
authors.lastname: Brown
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000154:Aconitate Hydratase / Q000378:metabolism; D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002952:Citric Acid Cycle / Q000502:physiology*; D005243:Feces / Q000469:parasitology; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D007700:Kinetics; D007814:Larva / Q000201:enzymology / Q000378:metabolism; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014251:Trichostrongyloidea / Q000201:enzymology / Q000378:metabolism*; D014252:Trichostrongyloidiasis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000105:Acetyl Coenzyme A; D007521:Isocitrate Dehydrogenase; C039355:isocitrate dehydrogenase (NADP+); D007655:Ketoglutarate Dehydrogenase Complex; D013385:Succinate Dehydrogenase; D002950:Citrate (si)-Synthase; D000154:Aconitate Hydratase