title: Selective removal of endotoxin from a DNA solution by cross-linked cyclodextrin beads.
abstract: The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 µg mL(-1), pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL(-1)), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and <1%, respectively.
authors.affiliation: Department of Applied Chemistry & Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. msakata@gpo.kumamoto-u.ac.jp
authors.forename: Masayo
authors.initials: M
authors.lastname: Sakata
authors.forename: Kana
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Itsumi
authors.initials: I
authors.lastname: Sakamoto
authors.forename: Masami
authors.initials: M
authors.lastname: Todokoro
authors.forename: Masashi
authors.initials: M
authors.lastname: Kunitake
mesh_terms: D000327:Adsorption; D002021:Buffers; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D008863:Microspheres; D009994:Osmolar Concentration; D012996:Solutions; D047392:beta-Cyclodextrins / Q000737:chemistry*; D047408:gamma-Cyclodextrins / Q000737:chemistry*
chemical_list: D002021:Buffers; D008070:Lipopolysaccharides; D012996:Solutions; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D004247:DNA