title: Simultaneous accumulation of hyaluronan binding protein 1 (HABP1/p32/gC1qR) and apoptotic induction of germ cells in cryptorchid testis.
abstract: In the experimentally cryptorchid rat, spermatogenic arrest is associated  with the formation of multinuclear giant cells, leading to large-scale  apoptosis and elimination of germ cells from the seminiferous epithelium.  Using this model, the role of Hyaluronan Binding Protein 1 (HABP1), which  expresses a stage specifically in post-meiotic cells during spermatogenesis,  was examined. Cryptorchidism induced complete arrest of spermatogenesis by 2  days, and by 3-5 days many large and small multinucleated giant cells  populated the affected tubules. Ultrastructure of the giant cells revealed  both single and multiple chromatin aggregation, with some less compact and  distorted, and others broken down into tiny fragments. These cells along with  other germ cells were stained terminal deoxynucleotidyl transferase dUTP  nick-end labeling positive, demonstrating strong expression of Bax and Heat  Shock Protein 70. Simultaneously, there was an up-regulation of the proprotein  form of HABP1 in these cells and a decrease in the mature form of protein. The  above findings indicate a possible role for HABP1 proprotein in apoptosis  induction of germ cells in the cryptorchid testes.
authors.affiliation: 103 Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: Man Mohan
authors.initials: MM
authors.lastname: Misro
authors.forename: Kasturi
authors.initials: K
authors.lastname: Datta
mesh_terms: D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D015153:Blotting, Western; D003456:Cryptorchidism / Q000378:metabolism* / Q000473:pathology; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D024101:Mitochondrial Proteins; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: C490195:C1qbp protein, rat; D017931:DNA Primers; D018960:Hyaluronan Receptors; D024101:Mitochondrial Proteins