title: Degradation of chloroform by immobilized cells of Bacillus sp. in calcium alginate beads.
abstract: A Bacillus sp., capable of degrading chloroform, was immobilized in calcium alginate. The beads in 20 g alginate l(-1) (about 2 × 10(8) cells/bead) could be re-used nine times for degradation of chloroform at 40 μM. The immobilized cells had a higher range of tolerance (pH 6.5-9 and 20-41°C) than free cells (pH 7-8.5 and 28-32°C). At 5 g alginate l(-1), leakage of the cells from the beads was 0.51 mg dry wt ml(-1). This species is the first reported Bacillus that can degrade chloroform as the sole carbon source.
authors.affiliation: Durgapur College of Commerce and Science, Rajbandh, Durgapur, Burdwan, 713212, West Bengal, India. kaushik_biotech@rediffmail.com
authors.forename: Kaushik
authors.initials: K
authors.lastname: Dey
authors.forename: Pranab
authors.initials: P
authors.lastname: Roy
mesh_terms: D000464:Alginates; D001407:Bacillus / Q000378:metabolism*; D058491:Bacterial Load; D001673:Biodegradation, Environmental; D001709:Biotechnology; D018914:Cells, Immobilized; D002725:Chloroform / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D002725:Chloroform; D020723:Glucuronic Acid