title: Collection of human genomic DNA from neonates: a comparison between umbilical cord blood and buccal swabs.
abstract: OBJECTIVE
  To compare DNA yield from neonatal umbilical cord blood and buccal swab specimens.
  
  
  METHODS
  Umbilical cord blood was obtained at birth in a cohort of women enrolled in a preterm labor study. If cord blood was not obtained, neonatal buccal samples were obtained using the Oragene saliva kits. DNA was extracted from all samples using the QIAamp extraction kits. DNA concentration and yield were compared between umbilical cord blood and buccal swabs.
  
  
  RESULTS
  DNA concentrations from umbilical cord blood (n = 35) was greater than that obtained from buccal swabs (n = 20) (total sample: 209.0 ± 110.7 ng/μL vs 6.9 ± 6.7 ng/μL respectively, P < .001; partial sample: n = 30 cord blood vs n = 11 buccal, 70.0 ± 51.4 ng/μL vs 11.3 ± 6.7 ng/μL, respectively, P < .001) and produced more total DNA (total sample: 116.5 ± 70.8 μg vs 4.2 ± 4.0 μg, P < .001; partial:14.0 ± 10.3 μg vs 1.1 ± 0.7 μg, respectively, P < .001).
  
  
  CONCLUSIONS
  Buccal swabs yield less neonatal DNA than umbilical cord blood specimens.
authors.affiliation: Division of Clinical Pharmacology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Amalia S
authors.initials: AS
authors.lastname: Lehmann
authors.forename: David M
authors.initials: DM
authors.lastname: Haas
authors.forename: Catherine L
authors.initials: CL
authors.lastname: McCormick
authors.forename: Todd C
authors.initials: TC
authors.lastname: Skaar
authors.forename: Jamie L
authors.initials: JL
authors.lastname: Renbarger
mesh_terms: D000367:Age Factors; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D016014:Linear Models; D011247:Pregnancy; D012463:Saliva / Q000166:cytology*
chemical_list: D004247:DNA