title: Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli).
abstract: Delta 6-fatty acid desaturase (D6DES) is used in the synthesis of polyunsaturated fatty acids (PUFAs) from microorganisms to higher animals, including arachidonic acid (ARA) and eicosapentaenoic acid (EPA). A 1,338 bp full-length cDNA encoding D6DES was cloned from Acanthopagrus schlegeli (AsD6DES) through degenerate- and RACE-PCR methods. A recombinant vector expressing AsD6DES (pYES-AsD6DES) was subsequently constructed and transformed into Saccharomyces cerevisiae to test the enzymatic activity of AsD6DES towards the production of n-6 and n-3 fatty acids. The exogenously expressed AsD6DES produced γ-linolenic acid (18:3 n-6) and stearidonic acid (18:4n-3) at 26 and 36% from exogenous linoleic acid (18:2n-6) and α-linolenic acid (18:3n-3), respectively, indicating that it is essentially a delta 6-fatty acid desaturase.
authors.affiliation: National Academy of Agricultural Science, Rural Development Administration, Seodun-Dong, Suwon, 441-707, Korea. sunheekim00@korea.kr
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Jong Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: So Yun
authors.initials: SY
authors.lastname: Kim
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Roh
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Kim
authors.forename: Kyeong-Ryeol
authors.initials: KR
authors.lastname: Lee
authors.forename: Yo Soon
authors.initials: YS
authors.lastname: Jang
authors.forename: Mi
authors.initials: M
authors.lastname: Kwon
authors.forename: Jong Sug
authors.initials: JS
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D005231:Fatty Acids, Unsaturated; D011994:Recombinant Proteins; D050602:Linoleoyl-CoA Desaturase