title: Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.
abstract: Cell culture is one of the most important methods of research in molecular and cellular biology, and various culture systems have been developed, including two-dimensional (2D), three-dimensional (3D) and floating culture systems. In the present study, we examined morphological changes and different expression patterns of cytoskeletal proteins in three different types of nervous system tumor cells grown in 2D, 3D and floating cell cultures. A172, KG-1-C and IMR-32 cells showed marked morphological changes, depending on the cell culture methods. F-actin expression was clearly observed at the level of the cells nearest the plate surface in 2D and 3D cultures. On the other hand, expression of F-actin was weak in the floating culture system. α-tubulin was detected in the cytoplasm of cells in 2D culture, but in floating and 3D cultures, α-tubulin was expressed in the peripheral regions of spheres and spheroids. In conclusion, this study demonstrated that nervous system tumor cells showed different alterations in morphology, and different cytoskeletal protein expression patterns, depending on the culture methods.
authors.affiliation: Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kawamoto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Teduka
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Peng
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishiwata
authors.forename: Zenya
authors.initials: Z
authors.lastname: Naito
mesh_terms: D000199:Actins / Q000032:analysis; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000032:analysis*; D006801:Humans; D009423:Nervous System Neoplasms / Q000737:chemistry / Q000473:pathology*; D018874:Spheroids, Cellular; D014404:Tubulin / Q000032:analysis
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D014404:Tubulin