title: Treatment of experimental autoimmune encephalomyelitis in SJL/J mice with a replicative HSV-1 vector expressing interleukin-5.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an autoimmune inflammation of the central nervous system and is used as the experimental model of multiple sclerosis (MS). The exact mechanism behind the disease is still unknown, but interleukin (IL)-17 expressing T cells are thought to mediate the disease. Toll-like receptors (TLRs) are known to have a role in the innate immune response against pathogens, and several TLRs have also a role in the disease course of EAE. Here, we show that treatment with a herpes simplex virus type 1 vector expressing the Th2 cytokine IL-5 ameliorates EAE and decreases the numbers of infiltrating lymphocytes in the brain. The effect involves downregulation of TLR 2, 3 and 9 mRNA expression and upregulation of type I interferons (IFNs) in brains during onset of disease. The elevated expression of type I IFNs was also observed during recovery.
authors.affiliation: Department of Virology, University of Turku, Turku, Finland. michaela.nygardas@utu.fi
authors.forename: M
authors.initials: M
authors.lastname: Nygårdas
authors.forename: C
authors.initials: C
authors.lastname: Aspelin
authors.forename: H
authors.initials: H
authors.lastname: Paavilainen
authors.forename: M
authors.initials: M
authors.lastname: Röyttä
authors.forename: M
authors.initials: M
authors.lastname: Waris
authors.forename: V
authors.initials: V
authors.lastname: Hukkanen
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D015536:Down-Regulation; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000628:therapy*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human / Q000235:genetics*; D007370:Interferon Type I / Q000378:metabolism; D015848:Interleukin-5 / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D007370:Interferon Type I; D015848:Interleukin-5; D012333:RNA, Messenger; D051193:Toll-Like Receptors