title: Mesoporous silica-coated hollow manganese oxide nanoparticles as positive T1 contrast agents for labeling and MRI tracking of adipose-derived mesenchymal stem cells.
abstract: Mesoporous silica-coated hollow manganese oxide (HMnO@mSiO(2)) nanoparticles were developed as a novel T(1) magnetic resonance imaging (MRI) contrast agent. We hypothesized that the mesoporous structure of the nanoparticle shell enables optimal access of water molecules to the magnetic core, and consequently, an effective longitudinal (R(1)) relaxation enhancement of water protons, which value was measured to be 0.99 (mM(-1)s(-1)) at 11.7 T. Adipose-derived mesenchymal stem cells (MSCs) were efficiently labeled using electroporation, with much shorter T(1) values as compared to direct incubation without electroporation, which was also evidenced by signal enhancement on T(1)-weighted MR images in vitro. Intracranial grafting of HMnO@mSiO(2)-labeled MSCs enabled serial MR monitoring of cell transplants over 14 days. These novel nanoparticles may extend the arsenal of currently available nanoparticle MR contrast agents by providing positive contrast on T(1)-weighted images at high magnetic field strengths.
authors.affiliation: Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Taeho
authors.initials: T
authors.lastname: Kim
authors.forename: Eric
authors.initials: E
authors.lastname: Momin
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Choi
authors.forename: Kristy
authors.initials: K
authors.lastname: Yuan
authors.forename: Hasan
authors.initials: H
authors.lastname: Zaidi
authors.forename: Jaeyun
authors.initials: J
authors.lastname: Kim
authors.forename: Mihyun
authors.initials: M
authors.lastname: Park
authors.forename: Nohyun
authors.initials: N
authors.lastname: Lee
authors.forename: Michael T
authors.initials: MT
authors.lastname: McMahon
authors.forename: Alfredo
authors.initials: A
authors.lastname: Quinones-Hinojosa
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
authors.forename: Taeghwan
authors.initials: T
authors.lastname: Hyeon
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003287:Contrast Media / Q000032:analysis* / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D017895:Manganese Compounds / Q000032:analysis* / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000032:analysis* / Q000737:chemistry; D012822:Silicon Dioxide / Q000032:analysis* / Q000737:chemistry
chemical_list: D003287:Contrast Media; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D012822:Silicon Dioxide