title: Synthesis and luminescence properties of new red-shifted absorption lanthanide(III) chelates suitable for peptide and protein labelling.
abstract: The synthesis and photo-physical properties of an original bis-pyridinylpyrazine chromophore efficiently sensitising europium(III) and samarium(III) are described. The corresponding lanthanide(III) complexes display in aqueous solutions a maximum excitation wavelength which is significantly red-shifted compared to the usual terpyridine-based chelates, and a valuable luminescence brightness above 2,000 dm(3) mol(-1) cm(-1) at 345 nm was obtained with a europium(III) derivative. Further functionalisation with three different bioconjugatable handles was also investigated and their ability to efficiently label a model hexapeptide was evaluated and compared. Finally, the best bioconjugatable europium(III) chelate was used in representative labelling experiments involving monoclonal antibodies and the luminescence features of the corresponding bioconjugates remained satisfactory.
authors.affiliation: Equipe de Chimie Bio-Organique, COBRA-CNRS UMR 6014 & FR 3038, rue Lucien Tesnière, 76131 Mont-Saint-Aignan, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Maindron
authors.forename: Séverine
authors.initials: S
authors.lastname: Poupart
authors.forename: Maxime
authors.initials: M
authors.lastname: Hamon
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Langlois
authors.forename: Nelly
authors.initials: N
authors.lastname: Plé
authors.forename: Ludovic
authors.initials: L
authors.lastname: Jean
authors.forename: Anthony
authors.initials: A
authors.lastname: Romieu
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D002614:Chelating Agents; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D008024:Ligands; D010455:Peptides; D011506:Proteins