title: Comparison of aryl hydrocarbon receptor gene expression in laser dissected granulosa cell layers of immature rat ovaries.
abstract: In order to understand ovarian toxicity of aryl hydrocarbon receptor (AhR) agonists, in situ gene expression of the AhR was examined during follicle development in immature rats. In situ hybridization on frozen sections of ovaries from 24-day-old Sprague-Dawley rats showed that the AhR mRNA was localized in the granulosa cells and occasionally in the theca cells of the follicles irrespective of the developmental stage. In situ gene quantification on granulosa cell layers collected by laser microdissection further revealed that the granulosa cells expressed less AhR mRNA according to development of belonging follicles, but more β-subunit of inhibin A mRNA, a quality control gene. These results may help to elucidate vulnerable developmental stages of follicles to toxicities of the AhR agonists.
authors.affiliation: Research Institute of Biosciences and High-Tech Research Center, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Sakurada
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Sawai
authors.forename: Kaoru
authors.initials: K
authors.lastname: Inoue
authors.forename: Mariko
authors.initials: M
authors.lastname: Shirota
authors.forename: Kinji
authors.initials: K
authors.lastname: Shirota
mesh_terms: D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006107:Granulosa Cells / Q000378:metabolism*; D017403:In Situ Hybridization / Q000662:veterinary; D061346:Laser Capture Microdissection / Q000662:veterinary; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018336:Receptors, Aryl Hydrocarbon / Q000096:biosynthesis* / Q000235:genetics; D013799:Theca Cells / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon