title: A novel zinc-finger-like gene from Tamarix hispida is involved in salt and osmotic tolerance.
abstract: In the present study, a zinc-finger-like cDNA (ThZFL) was cloned from the Tamarix hispida. Northern blot analysis showed that the expression of ThZFL can be induced by salt, osmotic stress and ABA treatment. Overexpression of the ThZFL confers salt and osmotic stress tolerance in both yeast Saccharomyces cerevisiae and tobacco. Furthermore, MDA levels in ThZFL transformed tobacco were significantly decreased compared with control plants under salt and osmotic stress, suggesting ThZFL may confer stress tolerance by decreasing membrane lipid peroxidation. Subcellular localization analysis showed the ThZFL protein is localized in the cell wall. Our results indicated the ThZFL gene is an excellent candidate for genetic engineering to improve salt and osmotic tolerance in agricultural plants.
authors.affiliation: Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Ministry of Education, Harbin 150040, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: An
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Lingling
authors.initials: L
authors.lastname: Lou
authors.forename: Tangchun
authors.initials: T
authors.lastname: Zheng
authors.forename: Guan-Zheng
authors.initials: GZ
authors.lastname: Qu
mesh_terms: D000595:Amino Acid Sequence; D015870:Gene Expression; D008315:Malondialdehyde / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000502:physiology; D012441:Saccharomyces cerevisiae; D054712:Salinity; D055049:Salt Tolerance; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D032363:Tamaricaceae / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000502:physiology; D014170:Transformation, Genetic; D016335:Zinc Fingers
chemical_list: D010940:Plant Proteins; D008315:Malondialdehyde