title: A new pathway for poly(3-hydroxybutyrate) production in Escherichia coli and Corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase.
abstract: A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] was developed in Escherichia coli and Corynebacterium glutamicum by an acetoacetyl-coenzyme A (CoA) synthase (AACS) recently isolated from terpenoid-producing Streptomyces sp. strain CL190. Expression of AACS led to significant productions of P(3HB) in E. coli (10.5 wt %) and C. glutamicum (19.7 wt %).
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Hokkaido, Japan.
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Miwa
authors.initials: M
authors.lastname: Yamada
authors.forename: Chean Ring
authors.initials: CR
authors.lastname: Leong
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Jo
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Kuzuyama
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
mesh_terms: D000214:Acyl Coenzyme A / Q000096:biosynthesis; D055162:Biocatalysis; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D006885:Hydroxybutyrates / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology; D000094122:Polyhydroxybutyrates
chemical_list: D000214:Acyl Coenzyme A; D003066:Coenzyme A Ligases; D006885:Hydroxybutyrates; D011091:Polyesters; C010667:acetoacetyl CoA; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C023747:acetoacetyl-CoA synthetase