title: Ecl1, a regulator of the chronological lifespan of Schizosaccharomyces pombe, is induced upon nitrogen starvation.
abstract: In fission yeast, ecl1(+) was identified as a novel factor that extends chronological lifespan when overexpressed. Ecl1 is a small protein consisting of 80 amino acids localized mainly in the nucleus. However, the mechanism by which it affects chronological lifespan has not been elucidated clearly. Here we analyzed the expression profile of Ecl1, especially as to cell cycle and growth phase, and found that it is induced upon nitrogen starvation. Then we analyzed the relevance of factors, Atf1, Ste11, and Tor1, which are known to be involved in the signaling of nitrogen starvation. Though the nitrogen starvation-induced expression of Ecl1 did not change in the atf1Δ mutant, induction in both the ste11Δ mutant and the tor1Δ mutant showed a delay. Based on these observations, the regulation of Ecl1 is discussed.
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Miwa
authors.forename: Hokuto
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Chikako
authors.initials: C
authors.lastname: Naito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Aiba
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D009584:Nitrogen / Q000172:deficiency*; D009687:Nuclear Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: C558592:Ecl1 protein, S pombe; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C523461:Tor1 protein, S pombe; C485941:ste13 protein, S pombe; D053487:DEAD-box RNA Helicases; D009584:Nitrogen