title: [Effects of NAIF1 on biological behavior of esophageal carcinoma cell line EC9706 by using pTet-off system].
abstract: OBJECTIVE
  To investigate the effects of NAIF1 on biological behavior of esophageal carcinoma cell line EC9706 by using pTet-off system.
  
  
  METHODS
  Constructe the pTRE2-NAIF1 plasmid and co-transfected with PTK-Hyg in human inducable esophageal carcinoma cell line TF93 (derive from EC9706 cell line).After Hygromycin B selection and RT-PCR, Western blot identification we got stable inducable cell clone TF93-pTRE2-NAIF1.By using this clone, we used MTT to test the proliferation rate, flow cytometry to test the cell cycle, Transwell chamber to test the migration ability and tested the adhesion ability of EC9706 under the effect of NAIF1.
  
  
  RESULTS
  First we got the stable inducable cell clone TF93-pTRE2-NAIF1, then we found NAIF1 could inhibit the cell proliferation, cause G0/G1 cell arrest and inhibit the migration and adhesion ability of EC9706.
  
  
  CONCLUSIONS
  NAIF1 can inhibit the malignant phenotype of esophageal carcinoma cell line EC9706.
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College; Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing 100021, China. luoqing730@126.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.forename: Jia-Ling
authors.initials: JL
authors.lastname: Zhong
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Chang-Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004938:Esophageal Neoplasms; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; C511414:NAIF1 protein, human; D009687:Nuclear Proteins