title: Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation.
abstract: Deoxyribonuclease I (DNase I) activity in serum has been shown to be a novel diagnostic marker for the early detection of acute myocardial infarction (AMI). However, the conventional method to measure DNase I activity is time-consuming. In the current study, to develop a rapid assay method for DNase I activity for clinical purposes, a microchip electrophoresis device was used to measure DNase I activity. Because DNase I is an endonuclease that degrades double-stranded DNA endo-nucleolytically to produce oligonucleotides, degradation of the DNA standard caused by DNase I action was detected using microchip electrophoresis. We detected DNase I activity within 10 min. This is the first study to apply microchip electrophoresis for the detection of DNase I activity; furthermore, it seems plausible that reduction of analysis time for DNase I activity could make this novel assay method using microchip electrophoresis applicable in clinical use.
authors.affiliation: Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Fujihara
authors.forename: Mari
authors.initials: M
authors.lastname: Tabuchi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Inoue
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Fujita
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
mesh_terms: D000208:Acute Disease; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D046209:Electrophoresis, Microchip / Q000379:methods*; D006801:Humans; D009203:Myocardial Infarction / Q000175:diagnosis
chemical_list: D004247:DNA; D003850:Deoxyribonuclease I