title: Chemomodulatory potential of Asparagus adscendens against murine skin and forestomach papillomagenesis.
abstract: We have evaluated the cancer chemopreventive efficacy of the roots of Asparagus adscendens, which have been used in the Indian traditional medicine system for a long time for the treatment of various ailments. For the first time, the effect of its different doses in a test diet was examined on 7,12-dimethylbenz(a)anthracene-induced skin and benzo(a)pyrene-induced forestomach papillomagenesis in mice. The effect of these test diets was also examined on drug-metabolizing phase I and phase II enzymes, antioxidant enzymes, reduced glutathione content, and peroxidative damage in mice. Results exhibited a significant reduction in the skin and the forestomach tumor incidence with respect to all the three (2, 4, and 6%, w/w) doses as compared with control. Among all the doses tested, 4% of test diet was most effective in protecting the animals against papillomagenesis. Further, the roots of A. adscendens inhibited phase I, and activated phase II system and antioxidant enzymes in the liver especially with 4% of test diet. The content of reduced glutathione was also significantly elevated whereas the peroxidative damage along with lactate dehydrogenase activity were reduced with all the three doses of the test diet. Together, these results suggest the cancer chemopreventive potential of A. adscendens, which could be mediated through drug-metabolizing phase I and phase II enzymes as well as free radical scavenging antioxidant enzymes.
authors.affiliation: Radiation and Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Singh
authors.forename: Surendra
authors.initials: S
authors.lastname: Singh
authors.forename: Raosaheb K
authors.initials: RK
authors.lastname: Kale
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D027761:Asparagus Plant / Q000737:chemistry*; D002273:Carcinogens / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D010212:Papilloma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D010936:Plant Extracts / Q000627:therapeutic use*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione