title: A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1.
abstract: Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.
authors.affiliation: Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Yazawa
authors.forename: Jun
authors.initials: J
authors.lastname: Takakura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sakata
authors.lastname: Ihsanawati
authors.forename: Rie
authors.initials: R
authors.lastname: Yatsunami
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Fukui
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D017434:Protein Structure, Tertiary; D014990:Xylans / Q000378:metabolism*
chemical_list: D014990:Xylans; D043364:Endo-1,4-beta Xylanases; D002118:Calcium