title: [Study on high-rate and low-rate metastatic large-cell lung cancer cell lines via differential display reverse transcript PCR].
abstract: BACKGROUND
  To investigate the differentially expressed gene profile between high-rate and low-rate metastatic large-cell lung cancer cell lines via differential display reverse transcript PCR (DDRT-PCR).
  
  
  METHODS
  DDRT-PCR was performed in two large-cell lung cancer cell lines with different metastatic ability. The differentially displayed cDNA were cloned and sequenced and compared with the sequences in the GenBank.
  
  
  RESULTS
  Fifty differentially displayed cDNAs were acquired, including 18 function-known genes, 16 function-unknown genes, 16 genomic DNA fragments and 4 ESTs.
  
  
  CONCLUSIONS
  The cell lines have different gene expression profiles, and metastasis is affected by multi-gene expression and regulation. Some genes which contribute to the metastasis of lung cancer could be found by DDRT-PCR, and this study gives some new clues to cancer diagnosis and therapy.
authors.affiliation: Department of Pulmonary Medicine, General Hospital of PLA, Beijing 100853, P.R.China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tian
authors.forename: Liang'an
authors.initials: L
authors.lastname: Chen
authors.forename: Youning
authors.initials: Y
authors.lastname: Liu