title: Targeting S100P inhibits colon cancer growth and metastasis by Lentivirus-mediated RNA interference and proteomic analysis.
abstract: S100P was recently found to be overexpressed in a variety of cancers and is considered a potential target for cancer therapy, but the functional role or mechanism of action of S100P in colon cancer is not fully understood. In the present study, we knocked down the gene expression of S100P in colon cancer cells using lentivirus-mediated RNA interference. This step resulted in significant inhibition of cancer cell growth, migration and invasion in vitro and tumor growth and liver metastasis in vivo. Moreover, S100P downstream target proteins were identified by proteomic analysis in colon cancer DLD-1 cells with deletion of S100P. Knockdown of S100P led to downregulation of thioredoxin 1 and β-tubulin and upregulation of Rho guanosine diphosphate (GDP) dissociation inhibitor α (RhoGDIA), all potential therapeutic targets in cancer. Taken together, these findings suggest that S100P plays an important role in colon tumorigenesis and metastasis, and the comprehensive and comparative analyses of proteins associated with S100P could contribute to understanding the downstream signal cascade of S100P, leading to tumorigenesis and metastasis.
authors.affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Yiu-Kay
authors.initials: YK
authors.lastname: Lai
authors.forename: Jinfang
authors.initials: J
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Marie Cm
authors.initials: MC
authors.lastname: Lin
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: He
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013879:Thioredoxins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D047368:Tumor Burden / Q000235:genetics; D015854:Up-Regulation; D023041:Xenograft Model Antitumor Assays; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
chemical_list: D002135:Calcium-Binding Proteins; D020730:Guanine Nucleotide Dissociation Inhibitors; D009363:Neoplasm Proteins; C103003:S100P protein, human; D014404:Tubulin; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D013879:Thioredoxins