title: Temporary increase of PPAR-γ and transient expression of UCP-1 in stromal vascular fraction isolated human adipocyte derived stem cells during adipogenesis.
abstract: In this study, cells from the stromal vascular fraction of human subcutaneous tissues were induced to differentiate toward adipose cells in vitro for 2 weeks. During adipogenic differentiation, we followed the chronological changes in their morphology with Coherent anti-Stokes Raman scattering (CARS) microscopy and checked the PPAR-γ and UCP-1 expression with RT-PCR. On day 4 after inducing adipogenic differentiation, CARS imaging showed multiple small lipid droplets (LD) distributed peripherally along the cellular membrane. PPAR-γ began to express at this time and increased until day 14 at a steady rate. On day 7, the cells appeared as brown adipocytes with numerous small LD throughout the cytoplasm, and the mRNA level of UCP-1 rose abruptly by 6- to 7-fold. After an additional 7 days, CARS imaging showed the development of a large LD, which is characteristic of white adipocytes, and the mRNA level of UCP-1 slumped significantly. These results demonstrate the possibility that ADSC pass through a brown adipocyte-like stage while differentiating into white adipocytes.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Yongon-dong, Chongno-gu, Seoul, Korea.
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Jo
authors.forename: Won Woo
authors.initials: WW
authors.lastname: Choi
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Park
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Moon
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Eun
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D047495:PPAR gamma; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1