title: Ligation induced matrix-metalloproteinase-9 activity in peripheral frog nervous system.
abstract: Matrix metalloproteinases (MMPs) are endopeptidases that cleave matrix, soluble and membrane-bound proteins and are regulated by their endogenous inhibitors the tissue inhibitors of MMPs (TIMPs). MMP-2 and MMP-9 are two of the MMPs which are essential to contribute to inflammatory and degenerative processes in injured nerves. The aim of the present study was to examine expression and activities of MMP-2 and MMP-9 in the injured and control groups frog sciatic nerves using gelatin zymography. Our investigation demonstrated for the first time as far as we know the expression of MMP-2 and MMP-9 in frog sciatic nerve. The expression and activity of MMP-9 were increased two fold on average following ligation. By contrast, MMP-2 activities remained unchanged. These findings suggest that we can consider MMP-9 as a marker for degenerative changes that follow nerve ligation in frog nerve.
authors.affiliation: Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, Université de la Méditerranée, Marseille, France.
authors.forename: Sihem
authors.initials: S
authors.lastname: Banasr
authors.forename: Oualid
authors.initials: O
authors.lastname: Sbai
authors.forename: Adlane
authors.initials: A
authors.lastname: Ould-Yahoui
authors.forename: Yatma
authors.initials: Y
authors.lastname: Gueye
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Hafedh
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D008026:Ligation / Q000379:methods*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008970:Molecular Weight; D011893:Rana esculenta; D012584:Sciatic Nerve / Q000201:enzymology* / Q000293:injuries
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9