title: [Autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine induces the cytotoxic T cell response in vitro].
abstract: BACKGROUND
  To investigate whether autologous lung cancer tissues derived gp96-peptide complex/dendritic cell vaccine could induce peptide specific cytotoxic T lymphocyte (CTL) response in vitro.
  
  
  METHODS
  A patient's tumor-derived antigens including gp96-peptide complexes and tumor cell lysate were co cultured with DCs derived from the same patient's bone marrow blood mononuclear cells. The various antigen/DC vaccines were used to stimulate peripheral lymphocytes. Interferon-γ (IFN-γ) level of activated lymphocytes was detected by ELISA method and the Cr51 release test was performed to evaluate the gp96-peptide specific CTL response in three kinds of target cells including the primary cultured tumor cells, PG cells and K562 cells.
  
  
  RESULTS
  IFN-γ could be observed from the supernate collected in all antigen groups after the cognate T lymphocytes were stimulated by various vaccines. The concentration of IFN-γ induced by gp96-peptide complexes/DC vaccine was higher than that of other groups. In addition, the killing effect of the activated T lymphocytes on patient's primary tumor cells was higher than that on PG and K562 cells.
  
  
  CONCLUSIONS
  Autologous tumor-derived gp96-peptide complexes can induce a peptide complex specific CTL response, and the CTL response is significantly intensified after DCs are pulsed.
authors.affiliation: Department of Thoracic Surgery, People's Hospital, Peking University, Beijing 100044, P.R.China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Danlei
authors.initials: D
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Cai
authors.forename: Chunrong
authors.initials: C
authors.lastname: Tong
authors.forename: Jun
authors.initials: J
authors.lastname: Wang