title: [Fluorescence-based determination of enzyme activity of recombinant CYPS1B1 (sterol 14alpha-demethylase) with coumarin derivatives].
abstract: The current investigation was undertaken with the aim to carry out an in vitro evaluation of the ability of coumarin derivatives as probe substrates to predict the activity of CYP51b1. The results obtained indicate that 7-aminocoumarin-4-acetic acid (ACAC) can be used to determine the recombinant CYP51b1 activity. Determination of CYP51b1 activity with ACAC is based on the direct registration of fluorescence increasing at 30 degrees C. It was found also that BMR in a simple soluble model system can be used as an electron donor for CYP51B1. Fluorescence-based assay is highly sensitive and can be used for the screening of sterol 14alpha-demethylase inhibitors.
authors.forename: N A
authors.initials: NA
authors.lastname: Petushkova
authors.forename: A V
authors.initials: AV
authors.lastname: Lisitsa
authors.forename: V F
authors.initials: VF
authors.lastname: Pozdnev
authors.forename: I I
authors.initials: II
authors.lastname: Karuzina
mesh_terms: D000818:Animals; D001410:Bacillus megaterium / Q000201:enzymology; D003374:Coumarins / Q000737:chemistry*; D005470:Fluorometry; D010084:Oxidation-Reduction; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D013045:Species Specificity; D058886:Sterol 14-Demethylase / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D003374:Coumarins; D011994:Recombinant Proteins; D058886:Sterol 14-Demethylase