title: [KeLuoXin medicated serum extract inhibits high glucose-induced proliferation and phosphorylation of ERK1/2 in rat mesangial cell].
abstract: OBJECTIVE
  To investigate the effects and mechanism of KeLuoXin (KLX) capsule on rat mesangial cell proliferation induced by high glucose.
  
  
  METHODS
  Rat mesangial cells (HBZY-1) were cultured in vitro and stimulated with high glucose(30 mmol/L glucose) for different time after the treatment with or without KLX medicated serum extract, the proliferation was assessed by cell counting Kit-8 (CCK-8) and the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot.
  
  
  RESULTS
  KLX could significantly inhibit HBZY-1 cell proliferation induced by high glucose (P < 0.05). In addition, KLX could reduce the increase of the phosphorylation level of ERK1/2 (P < 0.01), which was induced by high glucose in HBZY-1 cells.
  
  
  CONCLUSIONS
  KLX can inhibit rat mesangial cell proliferation, and the mechanisms may relate to the interruption of ERK1/2-Mitogen-activated protein kinase (MAPK) pathway.
authors.affiliation: Department of Pharmaceutical Preparation, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Li
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Ian-Ming
authors.initials: IM
authors.lastname: Wu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Li
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Gao
authors.forename: Yao
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum
chemical_list: D004365:Drugs, Chinese Herbal; D048049:Extracellular Signal-Regulated MAP Kinases; D005947:Glucose