title: [Separation, culture and purification of rat brain microendothelial cell].
abstract: OBJECTIVE
  To develop a separation method for brain microendothelial cells with the comparison of other ones.
  
  
  METHODS
  Twice enzymatic digestion and twice gradient centrifugation were applied to separate rat brain microendothelial cells. Then, immunomagnetic beads and Thy1.1 antibody were used respectively to purify the cultured cells.
  
  
  RESULTS
  Twice enzymatic digestion and twice gradient centrifugation could separate the cell successfully. High purification but low cell yield was obtained with immunomagnetic beads. The cells handled with Thy1.1 antibody had both higher purify coefficient and higher yield.
  
  
  CONCLUSIONS
  The developed method could separate the brain microendothelial cells successfully.
authors.affiliation: Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Lin-yu
authors.initials: LY
authors.lastname: Tian
authors.forename: Sheng-fu
authors.initials: SF
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000166:cytology*; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley