title: Development of ultra-high sensitive and selective electrochemiluminescent sensor for copper(II) ions: a novel strategy for modification of gold electrode using click chemistry.
abstract: A promising and highly sensitive electrochemiluminescence (ECL) sensor for the detection of Cu(2+) based on Cu(+)-catalyzed click reaction is described in this paper. Firstly, 1-azidoundecan-11-thiol was assembled on the Au electrode surface via a simple thiol-Au reaction, then the propargyl-functionalized Ru(bpy)(3)(2+)-doped SiO(2) nanoparticles (Ru-SNPs) ECL probe was covalently coupled on the electrode surfaces via click chemistry. Cu(+), the catalyst for click chemistry, is derived from the electrolytic reduction of Cu(2+)via the Bulk Electrolysis with coulometry (BE) technique and without any reductants. It is found that the ECL intensity detected from the electrode surface has a linear relationship with the logarithm of Cu(2+) concentration in the range of 1.0 × 10(-15) to 1.0 × 10(-11) M with a detection limit of 1.0 × 10(-16) M. Also, the method is highly specific even in the presence of high concentrations of other metal cations. It has been applied to detect trace Cu(2+) in complex samples (hepatoma cell) without sample treatment.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Suyan
authors.initials: S
authors.lastname: Qiu
authors.forename: Sen
authors.initials: S
authors.lastname: Gao
authors.forename: Xi
authors.initials: X
authors.lastname: Zhu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D002384:Catalysis; D045744:Cell Line, Tumor; D057930:Click Chemistry; D056831:Coordination Complexes / Q000737:chemistry; D003300:Copper / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D006046:Gold; D012822:Silicon Dioxide; D003300:Copper