title: Enzymatic and mutational analyses of a class II 3',5'-cyclic nucleotide phosphodiesterase, PdeE, from Myxococcus xanthus.
abstract: Myxococcus xanthus PdeE, an enzyme homologous to class II 3',5'-cyclic nucleotide phosphodiesterases, hydrolyzed cyclic AMP (cAMP) and cGMP with K(m) values of 12 μM and 25 μM, respectively. A pdeE mutant exhibited delays in fruiting body and spore formation compared with the wild type when cultured on starvation medium.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, Japan. kimura@ag.kagawa-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kimura
authors.forename: Masaaki
authors.initials: M
authors.lastname: Yoshimi
authors.forename: Goro
authors.initials: G
authors.lastname: Takata
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D003470:Culture Media / Q000737:chemistry; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004252:DNA Mutational Analysis; D007700:Kinetics; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D016941:Myxococcus xanthus / Q000201:enzymology* / Q000254:growth & development*; D016415:Sequence Alignment
chemical_list: D003470:Culture Media; D050505:Mutant Proteins; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D006152:Cyclic GMP