title: Evaluation of acetone as organic modifier in SPE for bioanalytical quantitative LC-MS/MS.
abstract: BACKGROUND
  To assess the suitability of acetone as an alternative to acetonitrile in SPE under otherwise commonly used conditions, with a focus on selectivity with regard to the most abundant phospholipid class. Two representative analytes were included, leuprolide and tramadol, a peptide and a small molecule, respectively.
  
  
  RESULTS
  The use of acetone resulted in analogous elution profiles of all monitored compounds in the majority of conditions. The only significant difference was on silica-based C18, where acetone effected markedly enhanced elution of lysophosphatidylcholines than did acetonitrile. Unmodified silica was shown to operate in per aqueous LC mode in highly aqueous conditions.
  
  
  CONCLUSIONS
  Acetone was established as a selectively similar and slightly more eluotropic alternative to acetonitrile in bioanalytical SPE.
authors.affiliation: Department of Bioanalytical Services, Huntingdon Life Sciences, Mettlers Road, East Millstone, NJ 08875-2360, USA. macneillr@princeton.huntingdon.com
authors.forename: Robert
authors.initials: R
authors.lastname: MacNeill
authors.forename: Esther
authors.initials: E
authors.lastname: Evans
mesh_terms: D000096:Acetone / Q000737:chemistry*; D000097:Acetonitriles / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D016729:Leuprolide / Q000032:analysis; D008244:Lysophosphatidylcholines / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D004364:Pharmaceutical Preparations / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014147:Tramadol / Q000032:analysis
chemical_list: D000097:Acetonitriles; D008244:Lysophosphatidylcholines; D004364:Pharmaceutical Preparations; D010713:Phosphatidylcholines; D000096:Acetone; D014147:Tramadol; D016729:Leuprolide; C032159:acetonitrile