title: Herbal medicines and infectious diseases: characterization by LC-SPE-NMR of some medicinal plant extracts used against malaria.
abstract: The extracts of two medicinal plants used in traditionalmedicine against malariawere characterized by means of an LC‑SPE‑NMR and LC‑MS platform. The structure of a series of major constituents from Bafodeya benna, as well as minor constituents from Ormocarpum kirkii, was determined. Bafodeya benna was found to contain (2R,3R)-taxifolin-3-O-α-L-rhamnoside or astilbin, and its isomers neoastilbin, neoisoastilbin, and isoastilbin, as well as quercetin-3-O-α-L-rhamnoside. From Ormocarpum kirkii, a series of known flavonoids and biflavonoids was obtained, as well as three new compounds, i.e., 7,7′′-di-O-β-D-glucosyl-(−)-chamaejasmin, 7-O-β-D-glucosyl-(I-3,II-3)-biliquiritigenin, and isovitexin-(I-3,II-3)-naringenin. The isolated constituents may explain, at least in part, the traditional use against malaria. LC‑SPE‑NMR, in combination with LC‑MS, is a powerful tool for the fast characterization of plant extracts, in order to define priorities at an early stage of a fractionation procedure. In addition, herbal medicinal products can completely be characterized, both with regard to their major as well as their minor constituents.
authors.affiliation: Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium.
authors.forename: Yong-Jiang
authors.initials: YJ
authors.lastname: Xu
authors.forename: Rica
authors.initials: R
authors.lastname: Capistrano
authors.forename: Liene
authors.initials: L
authors.lastname: Dhooghe
authors.forename: Kenn
authors.initials: K
authors.lastname: Foubert
authors.forename: Filip
authors.initials: F
authors.lastname: Lemière
authors.forename: Sheila
authors.initials: S
authors.lastname: Maregesi
authors.forename: Aliou
authors.initials: A
authors.lastname: Baldé
authors.forename: Sandra
authors.initials: S
authors.lastname: Apers
authors.forename: Luc
authors.initials: L
authors.lastname: Pieters
mesh_terms: D000962:Antimalarials / Q000737:chemistry; D044946:Biflavonoids / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D045469:Chrysobalanaceae / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D044948:Flavonols / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008288:Malaria / Q000188:drug therapy; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000962:Antimalarials; D044946:Biflavonoids; D005419:Flavonoids; D044948:Flavonols; D010936:Plant Extracts; C511440:chamaejasmine; C520363:neoastilbin; C099069:astilbin