title: Methods to evaluate alterations in polyamine metabolism caused by Helicobacter pylori infection.
abstract: Helicobacter pylori is a Gram-negative bacteria that infects the human stomach of half of the world's -population. Colonization is followed by infiltration of the gastric mucosa by lymphocytes and myeloid cells. These cells are activated by various bacterial factors, causing them to produce immune/inflammatory mediators, including reactive nitrogen species and polyamines that contribute to cellular damage and the pathogenesis of H. pylori-associated gastric cancer. In vitro experiments have revealed that H. pylori induces macrophage polyamine production by upregulation of the arginase 2/ornithine decarboxylase (ODC) metabolic pathway and enhances hydrogen peroxide synthesis through the activity of spermidine oxidase (SMO). In this chapter, we present a survey of the methods used to analyze the induction and the role of the enzymes related to polyamine metabolism, i.e., arginase, ODC, and SMO in H. pylori-infected macrophages.
authors.affiliation: UR454 Unite de Microbiologie, INRA, Saint-Genes-Champanelle, France.
authors.forename: Alain P
authors.initials: AP
authors.lastname: Gobert
authors.forename: Rupesh
authors.initials: R
authors.lastname: Chaturvedi
authors.forename: Keith T
authors.initials: KT
authors.lastname: Wilson
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001119:Arginase / Q000378:metabolism; D001152:Arsenicals / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D002478:Cells, Cultured; D057075:Enzyme Assays; D016481:Helicobacter Infections / Q000201:enzymology / Q000378:metabolism*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D008156:Luciferases / Q000378:metabolism; D008264:Macrophages / Q000201:enzymology / Q000382:microbiology; D051379:Mice; D009585:Nitrogen Dioxide / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase
chemical_list: D000123:Acetyltransferases; D001119:Arginase; D001152:Arsenicals; D008156:Luciferases; D009585:Nitrogen Dioxide; D009955:Ornithine Decarboxylase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D012333:RNA, Messenger; D000097667:Polyamine Oxidase; D000099228:Diamine N-Acetyltransferase; C065413:acetylaminophenylarsine oxide