title: DIGE-based proteomic analysis identifies nucleophosmin/B23 and nucleolin C23 as over-expressed proteins in relapsed/refractory acute leukemia.
abstract: Drug resistance is a challenge in treatment of acute leukemia. To investigate novel protein changes involved in resistance, protein expression profiles between leukemia cell line HL-60 and adriamycin-resistant HL-60 (HL-60/ADR) were compared based on a proteomic approach-2D-DIGE followed by MALDI-TOF/MS. 13 protein spots were identified as up-regulated and 3 down-regulated in HL-60/ADR. Nucleophosmin/B23 (NPM B23) and nucleolin C23 (C23) were selected and verified by western blot, which showed an obvious up-regulation in leukemia cells, especially in 3 resistant leukemia cell lines and in relapsed/refractory patients. To a conclusion, B23 and C23 may be involved in drug resistance and be useful in assessing the prognosis of leukemia.
authors.affiliation: Fujian Provincial Key Laboratory of Hematology, Fujian Institute of Hematology, Union Hospital of Fujian Medical University, Fuzhou, Fujian, China. jdhu@medmail.com.cn
authors.forename: Jianda
authors.initials: J
authors.lastname: Hu
authors.forename: Minhui
authors.initials: M
authors.lastname: Lin
authors.forename: Tingbo
authors.initials: T
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Buyuan
authors.initials: B
authors.lastname: Chen
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000378:metabolism*; D040901:Proteomics; D015996:Survival Rate; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured; D057825:Two-Dimensional Difference Gel Electrophoresis; D015854:Up-Regulation; D055815:Young Adult
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D010750:Phosphoproteins; C036825:phosphoprotein C23; D000090243:Nucleophosmin