title: The antioxidant activity of vitamin C, DPPD and L-cysteine against Cisplatin-induced testicular oxidative damage in rats.
abstract: Oxidative stress has been proven to be involved in cisplatin (CP)-induced toxicity. The present study was designed to evaluate the antioxidant activity of Vit C, N,N'-diphenyl-p-phenylenediamine (DPPD) and L-cysteine against CP-induced testicular oxidative damage in rats. Our data indicated significant increases in lipid peroxides (LPO), total peroxides and superoxide anion levels in testes of rats treated with CP (2 mg/kg/week, for 4 weeks) that was associated with a significant reduction in the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). The contents of glutathione (GSH), Vit E and Vit C were significantly lower in CP treated testes compared with these of control. The co-administration of CP with DPPD or L-cysteine significantly reduced the elevation in LPO, however the co-administration of CP with Vit C, DPPD or L-cysteine reduced the effect of CP on superoxide anion and antioxidant enzymes and also on the antioxidants contents. The administration of Vit C, DPPD or L-cysteine before CP injection improved the histological pictures and reduced the number of apoptotic cells and DPPD was more efficient. In conclusion, DPPD is a potent antioxidant, against CP-induced testicular oxidative damage, as compared with Vit C and L-cysteine.
authors.affiliation: Department of Zoology, Faculty of Science, Assiut University, Assiut 71516, Egypt.
authors.forename: Emad A
authors.initials: EA
authors.lastname: Ahmed
authors.forename: Hossam M
authors.initials: HM
authors.lastname: Omar
authors.forename: Sary Kh Abd
authors.initials: SKh
authors.lastname: elghaffar
authors.forename: Sohair M M
authors.initials: SM
authors.lastname: Ragb
authors.forename: Ahmed Y
authors.initials: AY
authors.lastname: Nasser
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers; D002945:Cisplatin / Q000009:adverse effects*; D003545:Cysteine / Q000494:pharmacology*; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010655:Phenylenediamines / Q000494:pharmacology*; D051381:Rats; D013481:Superoxides / Q000032:analysis; D013733:Testicular Diseases / Q000139:chemically induced* / Q000473:pathology; D013737:Testis / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D015415:Biomarkers; D010655:Phenylenediamines; D013481:Superoxides; C004845:N,N'-diphenyl-4-phenylenediamine; D003545:Cysteine; D001205:Ascorbic Acid; D002945:Cisplatin