title: In vitro antioxidant, lipoxygenase and xanthine oxidase inhibitory activities of fractions from Cienfuegosia digitata Cav., Sida alba L. and Sida acuta Burn f. (Malvaceae).
abstract: In this study polyphenol content, antioxidant activity, lipoxygenase (LOX) and Xanthine Oxidase (XO) inhibitory effects of n-hexane, dichloromethane, ethyl acetate and n-butanol fractions of aqueous acetone extracts from S. alba L., S. acuta Burn f and Cienfuegosia digitata Cav. were investigated. The total phenolics, flavonoids, flavonols and total tannins were determined by spectrophotometric methods using Folin-ciocalteu, AlCl3 reagents and tannic acid, respectively. The antioxidant potential was evaluated using three methods: inhibition of free radical 2,2-diphenyl-1-picrylhydramzyl (DPPH), ABTS radical cation decolorization assay and Iron (III) to iron (II) reduction activity (FRAP). For enzymatic activity, lipoxygenase and xanthine oxidase inhibitory activities were used. This study shows a relationship between polyphenol contents, antioxidant and enzymatic activities. Present results showed that ethyl acetate and dichloromethane fractions elicit the highest polyphenol content, antioxidant and enzymatic activities.
authors.affiliation: Laboratoire de Biochimie et Chimie Appliquées (LABIOCA), UFR/SVT, Université de Ouagadougou, 09 BP 848 Ouagadougou 09, Burkina Faso.
authors.forename: K
authors.initials: K
authors.lastname: Konaté
authors.forename: A
authors.initials: A
authors.lastname: Souza
authors.forename: A Y
authors.initials: AY
authors.lastname: Coulibaly
authors.forename: N T R
authors.initials: NT
authors.lastname: Meda
authors.forename: M
authors.initials: M
authors.lastname: Kiendrebeogo
authors.forename: A
authors.initials: A
authors.lastname: Lamien-Meda
authors.forename: J
authors.initials: J
authors.lastname: Millogo-Rasolodimby
authors.forename: M
authors.initials: M
authors.lastname: Lamidi
authors.forename: O G
authors.initials: OG
authors.lastname: Nacoulma
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D058945:Lipoxygenases / Q000187:drug effects*; D019660:Malvaceae / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D058945:Lipoxygenases; D014969:Xanthine Oxidase