title: Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum.
abstract: Validamycin A was used to inhibit in vivo trehalase activity in tobacco enabling the study of subsequent changes in new C partitioning into cellulosic biomass and lignin precursors. After 12-h exposure to treatment, plants were pulse labeled using radioactive (11)CO(2), and the partitioning of isotope was traced into [(11)C]cellulose and [(11)C]hemicellulose, as well as into [(11)C]phenylalanine, the precursor for lignin. Over this time course of treatment, new carbon partitioning into hemicellulose and cellulose was increased, while new carbon partitioning into phenylalanine was decreased. This trend was accompanied by a decrease in phenylalanine ammonia-lyase activity. After 4d of exposure to validamycin A, we also measured leaf protein content and key C and N metabolite pools. Extended treatment increased foliar cellulose and starch content, decreased sucrose, and total amino acid and nitrate content, and had no effect on total protein.
authors.affiliation: Fachbereich Chemie, Johannes Gutenberg Universität, 55099 Mainz, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Best
authors.forename: Kaitlyn
authors.initials: K
authors.lastname: Koenig
authors.forename: Kelly
authors.initials: K
authors.lastname: McDonald
authors.forename: Michael
authors.initials: M
authors.lastname: Schueller
authors.forename: Alistair
authors.initials: A
authors.lastname: Rogers
authors.forename: Richard A
authors.initials: RA
authors.lastname: Ferrieri
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D007294:Inositol / Q000031:analogs & derivatives / Q000494:pharmacology; D008031:Lignin / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D014198:Trehalase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D011134:Polysaccharides; D002245:Carbon Dioxide; C030790:validamycin A; D010649:Phenylalanine; D007294:Inositol; D002244:Carbon; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D014199:Trehalose; D014198:Trehalase; D010650:Phenylalanine Ammonia-Lyase