title: Infection dynamics and molecular identification of metacercariae in cyprinoids from Chiang Mai and Sakon Nakhon Provinces.
abstract: The infection dynamics of metacercariae were assessed in cyprinoid fish (cyprinoids) from Chiang Mai and Sakon Nakhon Provinces, Thailand, during October 2008 to September 2009. The samples were collected during 3 seasons from rivers and local markets. Metacercarial infection was determined by acid-pepsin digestion and confirmed using HAT-RAPD PCR method. Thirteen and 16 species of cyprinoids were collected from Chiang Mai and Sakon Nakhon with overall prevalences of metacercarial infection of 84.5 and 47.6%, respectively. Haplorchis taichui, Haplorchoides sp, and Centrocestus caninus were found in Chiang Mai and 4 species of metacercariae: H. taichui, Haplorchoides sp, O. viverrini and an unknown trematode species in Sakon Nakhon. H. taichui and Haplorchoides sp metacercariae in 3 species of cyprinoids (Henicorhynchus siamensis, Cyclocheilichthys armatus, Amblyrhynchichthy truncatus) had the highest prevalence (100%) in Chiang Mai, while the highest prevalence (100%) of metacercaria in Sakon Nakhon was Haplorchoides sp in 1 species of cyprinoids (Cyclocheilichthys armatus). The overall prevalence from Chiang Mai Province was highest in the rainy season (95.6%), lower in the hot-dry season (88.1%) and lowest in the cool season (72.5%). In Sakon Nakhon Province the highest prevalence was in the hot-dry season (52.7%), and lower in the rainy and cool season, 44.4% and 43.5%, respectively. The HAT-RAPD profiles confirmed the identity of metacercariae and adult stage of H. taichui, Haplorchoides sp, C. caninus and O. viverrini.
authors.affiliation: Department of Biology, Faculty of Science, Chiangmai University, Chiang Mai, Thailand. pheravut@yahoo.com
authors.forename: Pheravut
authors.initials: P
authors.lastname: Wongsawad
authors.forename: Chalobol
authors.initials: C
authors.lastname: Wongsawad
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000469:parasitology*; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D005819:Genetic Markers; D058488:Metacercariae / Q000302:isolation & purification* / Q000469:parasitology / Q000472:pathogenicity; D011159:Population Surveillance / Q000379:methods; D015995:Prevalence; D019105:Random Amplified Polymorphic DNA Technique; D013785:Thailand / Q000453:epidemiology; D014201:Trematode Infections / Q000453:epidemiology / Q000469:parasitology
chemical_list: D005819:Genetic Markers