title: Screening and bioguided fractionation of Amaryllidaceae species with anti-Trichomonas vaginalis activity.
abstract: The Amaryllidaceae family is known by its ornamental and medicinal value and has attracted considerable attention due to the content of alkaloids of its species, which showed interesting biological properties. The present study evaluated the anti-Trichomonas vaginalis activity of eighteen extracts (12.5 to 0.19 mg/mL) and six isolated alkaloids (125 to 1.9 µg/mL) from Amaryllidaceae species. The alkaloids diminished the trophozoites viability (from 15 to 40 %). The extracts from Hippeastrum breviflorum demonstrated the highest anti-T. vaginalis activity (viability was 60 % reduced), and a bioguided study was conducted. Six fractions with antiprotozoal activity had lycorine and lycosinine as major components suggesting a synergistic effect, taking into account the higher anti-T. vaginalis activity of extracts when compared to isolated alkaloids. Our results point out the antiprotozoal potential of the Amaryllidaceae species against T. vaginalis. This parasite causes trichomonosis, the most prevalent nonviral sexually transmitted diseases (STD) worldwide and a public health problem that requires new therapeutic alternatives as well bioactive natural products.
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Departamento de Análises, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Patrícia de Brum
authors.initials: Pde B
authors.lastname: Vieira
authors.forename: Raquel Brandt
authors.initials: RB
authors.lastname: Giordani
authors.forename: Geraldo Attilio
authors.initials: GA
authors.lastname: De Carli
authors.forename: José Angelo Silveira
authors.initials: JA
authors.lastname: Zuanazzi
authors.forename: Tiana
authors.initials: T
authors.lastname: Tasca
mesh_terms: D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors; D000241:Adenosine / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D047151:Amaryllidaceae Alkaloids / Q000737:chemistry / Q000494:pharmacology; D000994:Antitrichomonal Agents / Q000737:chemistry* / Q000494:pharmacology*; D005591:Chemical Fractionation; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D019668:Liliaceae / Q000737:chemistry*; D043583:Nucleoside-Triphosphatase / Q000037:antagonists & inhibitors; D010617:Phenanthridines / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D014246:Trichomonas vaginalis / Q000187:drug effects*; D053058:Trophozoites / Q000187:drug effects
chemical_list: D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D000994:Antitrichomonal Agents; D004791:Enzyme Inhibitors; D010617:Phenanthridines; D010936:Plant Extracts; C555185:candimine; C561828:lycosinine; D015720:5'-Nucleotidase; D043583:Nucleoside-Triphosphatase; C015330:lycorine; D000241:Adenosine