title: Development of a quinoxaline-based fluorescent probe for quantitative estimation of protein binding site polarity.
abstract: The compound 2-[(1E)-2-(1H-pyrrol-2-yl)ethenyl]-quinoxaline (PQX) is a promising fluorescent chromophore for the estimation of protein binding site polarity, due to its full-color solvatochromic fluorescence. A linear relationship was obtained between the peak emission wavenumber and E(T)(N) (normalized solvent polarity). The BSA binding site polarity was estimated from the solvatochromic plot.
authors.affiliation: Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kudo
authors.forename: Atsuya
authors.initials: A
authors.lastname: Momotake
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kanna
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Arai
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry*; D011485:Protein Binding; D011810:Quinoxalines / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011810:Quinoxalines; D012710:Serum Albumin, Bovine