title: Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.
abstract: We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Zengyi
authors.initials: Z
authors.lastname: Shao
authors.forename: Yunzi
authors.initials: Y
authors.lastname: Luo
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001688:Biological Products / Q000235:genetics*; D053898:Biosynthetic Pathways / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D058615:Synthetic Biology
chemical_list: D001688:Biological Products