title: RGD-targeted paramagnetic liposomes for early detection of tumor: in vitro and in vivo studies.
abstract: Magnetic resonance molecular imaging has emerged as a potential approach for tumor diagnosis in the last few decades. This approach consists of the delivery of MR contrast agents to the tumor by specific targeted carriers. For this purpose, a lipopeptide was constructed by using a cyclic RGD peptide headgroup coupled to palmitic acid anchors via a KGG tripeptide spacer. Targeted paramagnetic liposomes were then prepared by the incorporation of RGD-coupled-lipopeptides into lipid bilayers for specific bounding to tumor. In vitro, study demonstrated that RGD-targeted liposomes exhibited a better binding affinity to targeted cells than non-targeted liposomes. MR imaging of mice bearing A549 tumors with the RGD-targeted paramagnetic liposomes also resulted in a greater signal enhancement of tumor compared to non-targeted liposomes and pure contrast agents groups. In addition, biodistribution study also showed specific tumor targeting of RGD-targeted paramagnetic liposomes in vivo. Therefore, RGD-targeted paramagnetic liposomes prepared in the present study may be a more promising method for early tumor diagnosis.
authors.affiliation: Research Institute of Oncology, Tongji University Medical School, 507 Zhenmin Road, Shanghai 200433, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Su
authors.forename: Shuyan
authors.initials: S
authors.lastname: Meng
authors.forename: Lixia
authors.initials: L
authors.lastname: Ju
authors.forename: Linghua
authors.initials: L
authors.lastname: Yan
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Ding
authors.forename: Yin
authors.initials: Y
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Heyan
authors.initials: H
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Yinmin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Caicun
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D000704:Analysis of Variance; D000818:Animals; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D019786:Gadolinium DTPA / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes / Q000138:chemical synthesis*; D008175:Lung Neoplasms / Q000175:diagnosis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014183:Transplantation, Heterologous
chemical_list: D008081:Liposomes; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D019786:Gadolinium DTPA