title: T rosette assay: in vitro correlate of cellular immunity.
abstract: The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.
authors.affiliation: James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, Maryland 21205, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Catalona
authors.forename: C
authors.initials: C
authors.lastname: Potvin
authors.forename: J L
authors.initials: JL
authors.lastname: Tarpley
authors.forename: P B
authors.initials: PB
authors.lastname: Chretien
mesh_terms: D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D012397:Rosette Formation; D014407:Tumor Cells, Cultured; D014571:Urologic Neoplasms / Q000276:immunology*