title: Functional preconcentration tip of total volume injection for ESI/MS analysis of DNA adducts.
abstract: We have developed a simple method to significantly improve the sensitivity in the LC/MS analysis of DNA adducts. A preconcentration tip for the selective recovery of DNA adducts was prepared. Using this tip, the total amount of DNA adducts in a treated DNA sample was injected in a one-shot manner into an LC/MS system. We were able to improve the sensitivity by more than one order of magnitude in concentration. This method will be a useful tool for the quantitative determination of trace DNA adducts.
authors.affiliation: Gifu Pharmaceutical University, Daigaku-nishi, Gifu 501-1196, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Murakami
authors.forename: Mio
authors.initials: M
authors.lastname: Koguchi
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Esaka
authors.forename: Bunji
authors.initials: B
authors.lastname: Uno
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishihama
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry; D007267:Injections; D009711:Nucleotides / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D018736:DNA Adducts; D009711:Nucleotides