title: Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
abstract: The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-α. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.
authors.affiliation: Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain. mariona.gimeno@cresa.uab.cat.
authors.forename: Mariona
authors.initials: M
authors.lastname: Gimeno
authors.forename: Laila
authors.initials: L
authors.lastname: Darwich
authors.forename: Ivan
authors.initials: I
authors.lastname: Diaz
authors.forename: Eugenia
authors.initials: E
authors.lastname: de la Torre
authors.forename: Joan
authors.initials: J
authors.lastname: Pujols
authors.forename: Marga
authors.initials: M
authors.lastname: Martín
authors.forename: Shigeki
authors.initials: S
authors.lastname: Inumaru
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Cano
authors.forename: Mariano
authors.initials: M
authors.lastname: Domingo
authors.forename: Maria
authors.initials: M
authors.lastname: Montoya
authors.forename: Enric
authors.initials: E
authors.lastname: Mateu
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism*; D005786:Gene Expression Regulation; D010641:Phenotype; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics* / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D013552:Swine
chemical_list: D016207:Cytokines