title: Crystallization of calcium carbonate on chitosan substrates in the presence of regenerated silk fibroin.
abstract: The crystallization of calcium carbonate (CaCO3) was investigated using a mineralization system composed of a chitosan membrane and regenerated silk fibroin (RSF). Such a system may resemble the mineralization in molluscs, where chitosan is a derivative of chitin and RSF an analogue of nacreous protein. It was found that the vaterite disks generally formed on the chitosan membrane while the aragonite disks also appeared with changes of pH value or temperature of the solution. The crystallization of CaCO3 in the vicinity of the chitosan membrane was much more affected by the environment of crystallization, compared to that in bulk solution. Detailed observation from high-resolution scanning and transmission electron microscopy (HRSEM and TEM) showed that these disks consisted of nanoparticles about 20 nm in size, thus suggesting that the accumulation of hybrid CaCO3/RSF nanoparticles induced the formation of crystalline disks on the chitosan membrane.
authors.affiliation: Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Laboratory of Advanced Materials, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors.forename: Yaodong
authors.initials: Y
authors.lastname: Wu
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.forename: Jinrong
authors.initials: J
authors.lastname: Yao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003460:Crystallization; D005349:Fibroins / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D005349:Fibroins; D048271:Chitosan; D002119:Calcium Carbonate