title: Blockade of IL-6 secretion pathway by the sesquiterpenoid atractylenolide III.
abstract: Atractylenolide III (1) is the major bioactive component of Atractylodes lancea. The aim of this study was to analyze the effect on the regulation of interleukin (IL)-6 secretion pathway caused by 1. This sesquiterpenoid inhibited the secretion and expression of IL-6 in phorbol 12-myristate 13-acetate- and calcium ionophore A23187-stimulated human mast cells (HMC)-1. In addition, 1 inhibited histamine release in stimulated HMC-1 cells. In stimulated HMC-1 cells, 1 suppressed activation of p38 mitogen-activated protein kinase, C-Jun-N-terminal protein kinase, and nuclear factor-κB. In addition, 1 suppressed the activation of caspase-1 and the expression of receptor interacting protein-2. These results provide new insights that atractylenolide III (1) may control immunological reactions by regulating the cellular functions of IL-6 in mast cells.
authors.affiliation: Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Kang
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
mesh_terms: D020170:Caspase 1 / Q000378:metabolism; D061945:Caspase Inhibitors; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D007783:Lactones / Q000737:chemistry / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D015394:Molecular Structure; D016328:NF-kappa B / Q000187:drug effects; D012717:Sesquiterpenes / Q000737:chemistry / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D000093242:Interleukin-6 Inhibitors
chemical_list: D020170:Caspase 1; D061945:Caspase Inhibitors; D006632:Histamine; D015850:Interleukin-6; D048031:JNK Mitogen-Activated Protein Kinases; D007783:Lactones; D016328:NF-kappa B; D012717:Sesquiterpenes; D048051:p38 Mitogen-Activated Protein Kinases; D000093242:Interleukin-6 Inhibitors; C424802:atractylenolide III