title: Experimental infection of goats with tick-borne encephalitis virus and the possibilities to prevent virus transmission by raw goat milk.
abstract: OBJECTIVE
  The aim of this work was to study the tick-borne encephalitis virus (TBEV) infection of goats and the possibilities to prevent human milk-borne infections either by immunizing animals or the heat treatment of milk.
  
  
  METHODS
  An experiment was conducted with 20 milking goats. Ten goats (half of them immunized) were challenged with live TBEV and 10 were left uninfected. Clinical signs and body temperatures of the animals were recorded and milk samples were collected daily. The presence of viral RNA and infectious virions in milk were detected by RT-PCR and intracerebral inoculation of suckling mice, respectively. Milk samples containing infectious virions were subjected to various heat treatment conditions and retested afterwards to assess the effect on infectivity.
  
  
  RESULTS
  The infected goats did not show any clinical signs or fever compared to uninfected ones. Infectious virions were detected for 8-19 days from the milk samples (genome for 3-18 days by PCR) of infected goats. Immunized goats did not shed the virus. After heat treatment of the milk, the inoculated mice survived.
  
  
  CONCLUSIONS
  Goats shed the virus with their milk without showing any symptoms. Human milk-borne infections can be avoided both by immunizing goats and boiling/pasteurizing infected milk.
authors.affiliation: National Center for Epidemiology, Budapest, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Balogh
authors.forename: László
authors.initials: L
authors.lastname: Egyed
authors.forename: Emőke
authors.initials: E
authors.lastname: Ferenczi
authors.forename: Enikő
authors.initials: E
authors.lastname: Bán
authors.forename: Katalin N
authors.initials: KN
authors.lastname: Szomor
authors.forename: Mária
authors.initials: M
authors.lastname: Takács
authors.forename: György
authors.initials: G
authors.lastname: Berencsi
mesh_terms: D000818:Animals; D004669:Encephalitis Viruses, Tick-Borne / Q000302:isolation & purification / Q000472:pathogenicity*; D004675:Encephalitis, Tick-Borne / Q000473:pathology / Q000635:transmission* / Q000662:veterinary* / Q000821:virology; D006041:Goats / Q000821:virology*; D051379:Mice; D008892:Milk / Q000821:virology*; D061352:Pasteurization / Q000379:methods; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017201:Virus Shedding; D015047:Zoonoses / Q000635:transmission*
chemical_list: D012367:RNA, Viral