title: PCR for the diagnosis and species identification of microsporidia in patients with keratitis.
abstract: Corneal scrapings from 30 patients with microbial keratitis were subjected to microsporidial PCR. PCR was positive for microsporidia in ten of 30 patients. The species was identified as Vittaforma corneae by sequencing in all ten patients. The remaining 20 patients were negative for microsporidia and showed the growth of other organisms (Acanthamoeba, fungi or bacteria).
authors.affiliation: Jhaveri Microbiology Centre, Hyderabad Eye Research Foundation, India. ashokkumar@lvpei.org
authors.forename: A K
authors.initials: AK
authors.lastname: Reddy
authors.forename: P K
authors.initials: PK
authors.lastname: Balne
authors.forename: K
authors.initials: K
authors.lastname: Gaje
authors.forename: P
authors.initials: P
authors.lastname: Garg
mesh_terms: D007634:Keratitis / Q000382:microbiology*; D016814:Microsporidia / Q000145:classification / Q000235:genetics*; D016881:Microsporidiosis / Q000175:diagnosis*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D054682:Ribosome Subunits, Small, Eukaryotic / Q000235:genetics