title: A complex adenovirus vaccine against chikungunya virus provides complete protection against viraemia and arthritis.
abstract: Chikungunya virus, a mosquito-borne alphavirus, recently caused the largest epidemic ever seen for this virus. Chikungunya disease primarily manifests as a painful and debilitating arthralgia/arthritis, and no effective drug or vaccine is currently available. Here we describe a recombinant chikungunya virus vaccine comprising a non-replicating complex adenovirus vector encoding the structural polyprotein cassette of chikungunya virus. A single immunisation with this vaccine consistently induced high titres of anti-chikungunya virus antibodies that neutralised both an old Asian isolate and a Réunion Island isolate from the recent epidemic. The vaccine also completely protected mice against viraemia and arthritic disease caused by both virus isolates.
authors.affiliation: Division of Biodefense Vaccines, GenPhar, Inc., Mount Pleasant, SC 29464, USA.
authors.forename: Danher
authors.initials: D
authors.lastname: Wang
authors.forename: Andreas
authors.initials: A
authors.lastname: Suhrbier
authors.forename: Adam
authors.initials: A
authors.lastname: Penn-Nicholson
authors.forename: Jan
authors.initials: J
authors.lastname: Woraratanadharm
authors.forename: Joy
authors.initials: J
authors.lastname: Gardner
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.forename: Thuy T
authors.initials: TT
authors.lastname: Le
authors.forename: Itaru
authors.initials: I
authors.lastname: Anraku
authors.forename: Michael
authors.initials: M
authors.lastname: Sakalian
authors.forename: David
authors.initials: D
authors.lastname: Einfeld
authors.forename: John Y
authors.initials: JY
authors.lastname: Dong
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D018354:Alphavirus Infections / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001168:Arthritis / Q000276:immunology / Q000517:prevention & control*; D002646:Chikungunya virus / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers; D005260:Female; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014766:Viremia / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D014765:Viral Vaccines