title: [Studies on the killing effect of gamma ray irradiated peripheral blood mononuclear cells combined with (-)-gossypol on human prostate cancer LNCaP cells].
abstract: OBJECTIVE
  To observe the killing effect of irradiated peripheral blood mononuclear cells (PBMCs) at low dose combined with (-)-gossypol on cultured human prostate cancer LNCaP cells.
  
  
  METHODS
  Human PBMCs were irradiated by gamma ray at 1 gray, the irradiation dose rate was 17 Gy/min. The experiments were divided into control group (LNCaP cells only), LNCaP cells with irradiated and non-irradiated PBMCs co-culture groups, (-)-gossypol treatment group, irradiated PBMCs and (-)-gossypol co-treatment group. Acridine orange/ethidium bromide (AO/EB) staining and MTT method were used to observe the killing effect of PBMCs and/or (-)-gossypol.
  
  
  RESULTS
  The killing activity of irradiated PBMCs group and (-)-gossypol treatment group were obviously increased and were higher than that of non-irradiated group (P < 0.05). The killing activity of combined group was much higher than that of irradiated group and ApoG2 treatment group (P < 0.01).
  
  
  CONCLUSIONS
  Irradiated PBMCs at low dose combined with (-)-gossypol can enhance the anti-tumor effects obviously.
authors.affiliation: Department of Radiation Oncology, Affiliated Hospital of Ningxia Medical University, Yinchuan, China.
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Zhan
authors.forename: Yu-xiang
authors.initials: YX
authors.lastname: Zhan
authors.forename: Gang
authors.initials: G
authors.lastname: Yan
authors.forename: Xian-qing
authors.initials: XQ
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D005720:Gamma Rays; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000528:radiation effects*; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D006072:Gossypol