title: A metabolomics and proteomics study of the adaptation of Staphylococcus aureus to glucose starvation.
abstract: As a versatile pathogen Staphylococcus aureus can cause various disease patterns, which are influenced by strain specific virulence factor repertoires but also by S. aureus physiological adaptation capacity. Here, we present metabolomic descriptions of S. aureus central metabolic pathways and demonstrate the potential for combined metabolomics- and proteomics-based approaches for the basic research of this important pathogen. This study provides a time-resolved picture of more than 500 proteins and 94 metabolites during the transition from exponential growth to glucose starvation. Under glucose excess, cells exhibited higher levels of proteins involved in glycolysis and protein-synthesis, whereas entry into the stationary phase triggered an increase of enzymes of TCC and gluconeogenesis. These alterations in levels of metabolic enzymes were paralleled by more pronounced changes in the concentrations of associated metabolites, in particular, intermediates of the glycolysis and several amino acids.
authors.affiliation: Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, F.-L.-Jahn-Str. 17, D-17487 Greifswald, Germany.
authors.forename: Manuel
authors.initials: M
authors.lastname: Liebeke
authors.forename: Kirsten
authors.initials: K
authors.lastname: Dörries
authors.forename: Daniela
authors.initials: D
authors.lastname: Zühlke
authors.forename: Jörg
authors.initials: J
authors.lastname: Bernhardt
authors.forename: Stephan
authors.initials: S
authors.lastname: Fuchs
authors.forename: Jan
authors.initials: J
authors.lastname: Pané-Farré
authors.forename: Susanne
authors.initials: S
authors.lastname: Engelmann
authors.forename: Uwe
authors.initials: U
authors.lastname: Völker
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Bode
authors.forename: Thomas
authors.initials: T
authors.lastname: Dandekar
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lindequist
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Michael
authors.initials: M
authors.lastname: Lalk
mesh_terms: D000222:Adaptation, Physiological; D016000:Cluster Analysis; D017077:Culture Media, Conditioned / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D040901:Proteomics; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned; D005947:Glucose