title: Suppression of TSPAN1 by RNA interference inhibits proliferation and invasion of colon cancer cells in vitro.
abstract: OBJECTIVE
  To investigate effect ofTSPAN1 downregulation by RNA interference (RNAi) on proliferation and invasion of human colon cancer cells in vitro.
  
  
  METHODS
  RNAi was performed using the vector (pU6H1-GFP)-based small-interfering RNA (siRNA) plasmid gene silencing system to specifically knock down TSPAN1 expression in a colon cancer cell line, HCT-8. The expression of TSPAN1 mRNA was detected by reverse-transcription polymerase chain reaction. TSPAN1 protein expression was observed using Western blots and immunofluorescent microscopy. Cell proliferation and cell cycle assay were measured using methyl thiazolyl tetrazolium (MTT) and flow cytometry, respectively. The invasive ability of HCT-8 cells was examined using a duel culture chamber separated by polycarbonate membranes coated with Matrigel (8.0-microm pore size).
  
  
  RESULTS
  After transfection with the TSPAN1 siRNA plasmid, TSPAN1 mRNA and protein expression was significantly decreased. The decrease in mRNA and protein was associated with a significant decrease in TSPAN1 fluorescent staining and a decrease in cell proliferation due to cell cycle arrest in the G1/G0 phase. A significant decrease in the number of invading HCT-8 cells was associated with these changes.
  
  
  CONCLUSIONS
  RNAi-mediated downregulation of TSPAN1 expression significantly inhibits the proliferation and invasion of colon cancer cells in vitro. This finding suggests that TSPAN1 plays an important role in colon cancer progression, and RNAi targeting of TSPAN1 may be a potential therapeutic strategy for the treatment of colon cancer.
authors.affiliation: Department of Pathological Anatomy Medical School of Nantong University, Nantong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Daiyue
authors.initials: D
authors.lastname: Yuan
authors.forename: Ren
authors.initials: R
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhu
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D015536:Down-Regulation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009361:Neoplasm Invasiveness; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059470:Tetraspanins; D014162:Transfection
chemical_list: D008565:Membrane Proteins; D034741:RNA, Small Interfering; C508125:TSPAN1 protein, human; D059470:Tetraspanins