title: Effect of the Synadenium carinatum latex lectin (ScLL) on Leishmania (Leishmania) amazonensis infection in murine macrophages.
abstract: Antiparasitic effect of a lectin isolated from Synadenium carinatum latex (ScLL) was evaluated against Leishmania (Leishmania) amazonensis promastigotes/amastigotes. Pretreatment of murine inflammatory peritoneal macrophages with ScLL reduced by 65.5% the association index of macrophages and L. (L) amazonensis promastigotes. Expression of cytokines (IL-12, IL-1 and TNF-α) was detected in infected macrophages pretreated with ScLL (10μg/mL). ScLL also reduced the growth of L. (L) amazonensis amastigote intracellular forms, showing no in vitro cytotoxic effects in mammalian host cells. ScLL treatment in infected murine inflammatory peritoneal macrophages did not induce nitric oxide production, suggesting that a nitric oxide independent pathway is activated to decrease the number of intracellular Leishmania.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, Uberlândia, MG, Brazil.
authors.forename: Sandra R
authors.initials: SR
authors.lastname: Afonso-Cardoso
authors.forename: Claudio Vieira
authors.initials: CV
authors.lastname: Silva
authors.forename: Marcelo S
authors.initials: MS
authors.lastname: Ferreira
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souza
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D019665:Euphorbiaceae / Q000737:chemistry*; D007840:Latex / Q000494:pharmacology* / Q000627:therapeutic use; D007894:Leishmania mexicana / Q000187:drug effects*; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis / Q000235:genetics; D037121:Plant Lectins / Q000494:pharmacology* / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D007840:Latex; D037121:Plant Lectins; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II