title: [Preparation of ATP-2Na loaded liposome and its effect on tissues energy state in myocardial ischemic mice].
abstract: The aim of this study is to improve liposome encapsulation efficiency of water soluble drug ATP-2Na with hydrophobic ion pairing method, and evaluate its effect on tissues energy state in myocardial ischemia mice. Ion pair complex of ATP-2Na with HTAB was prepared first; then the liposomes were prepared by ethanol injection method. The size and zeta potential of ATP-2Na liposome were investigated. Its effect on tissues energy state in myocardium ischemia mice was evaluated by detecting ATP-2Na concentration in tissues and blood after injection in comparison to ATP-2Na solution. The diameters and zeta potential of ATP-2Na liposomes were (144.0 +/- 2.7) nm and (+16.2 +/- 1.6) mV, respectively. The encapsulation efficiency was (85.02 +/- 2.31) %. The in vitro drug release pattern from liposomes matches with Weibull equation. Compared with ATP-2Na solution, ATP-2Na liposome increased the ATP concentration of blood in myocardial ischemic mice very significantly; compared with blank, ATP-2Na liposome increased ATP content of myocardium and liver in myocaridal ischemic mice significantly, but ATP-2Na solution didn't show this effect. ATP-2Na liposome might have an advantage in improving tissue energy state on myocaridal ischemic animals.
authors.affiliation: China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Feng-mei
authors.initials: FM
authors.lastname: Pi
authors.forename: Xi-de
authors.initials: XD
authors.lastname: Tu
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000255:Adenosine Triphosphate / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*; D000818:Animals; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004337:Drug Carriers; D008081:Liposomes / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D017202:Myocardial Ischemia / Q000097:blood / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010316:Particle Size; D011897:Random Allocation; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D004337:Drug Carriers; D008081:Liposomes; D013501:Surface-Active Agents; D000255:Adenosine Triphosphate; D000077286:Cetrimonium