title: Preparation of surface plasmon resonance biosensor based on magnetic core/shell Fe3O4/SiO2 and Fe3O4/Ag/SiO2 nanoparticles.
abstract: In this paper, surface plasmon resonance biosensors based on magnetic core/shell Fe(3)O(4)/SiO(2) and Fe(3)O(4)/Ag/SiO(2) nanoparticles were developed for immunoassay. With Fe(3)O(4) and Fe(3)O(4)/Ag nanoparticles being used as seeding materials, Fe(3)O(4)/SiO(2) and Fe(3)O(4)/Ag/SiO(2) nanoparticles were formed by hydrolysis of tetraethyl orthosilicate. The aldehyde group functionalized magnetic nanoparticles provide organic functionality for bioconjugation. The products were characterized by scanning electronic microscopy (SEM), transmission electronic microscopy (TEM), FTIR and UV-vis absorption spectrometry. The magnetic nanoparticles possess the unique superparamagnetism property, exceptional optical properties and good compatibilities, and could be used as immobilization matrix for goat anti-rabbit IgG. The magnetic nanoparticles can be easily immobilized on the surface of SPR biosensor chip by a magnetic pillar. The effects of Fe(3)O(4)/SiO(2) and Fe(3)O(4)/Ag/SiO(2) nanoparticles on the sensitivity of SPR biosensors were also investigated. As a result, the SPR biosensors based on Fe(3)O(4)/SiO(2) nanoparticles and Fe(3)O(4)/Ag/SiO(2) nanoparticles exhibit a response for rabbit IgG in the concentration range of 1.25-20.00 μg ml(-1) and 0.30-20.00 μg ml(-1), respectively.
authors.affiliation: College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Daqian
authors.initials: D
authors.lastname: Song
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007136:Immunoglobulins / Q000032:analysis; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; D007136:Immunoglobulins; D012834:Silver; D012822:Silicon Dioxide