title: EBV-encoded LMP1 increases nuclear β-catenin accumulation and its transcriptional activity in nasopharyngeal carcinoma.
abstract: This paper aimed to study whether Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) regulates β-catenin signaling pathway in nasopharyngeal carcinoma (NPC). Western blotting, immunofluorescence, luciferase reporter assay, co-immunoprecipitation assay, and immunohistochemistry staining were used. LMP1 increased β-catenin transcriptional activity in NPC cell lines. The upregulation of β-catenin transcriptional activity induced by LMP1 was much higher in poorly differentiated NPC cell line CNE2 than that in well-differentiated NPC cell line CNE1. Immunofluorescence staining and Western blotting also showed that LMP1 increased nuclear β-catenin accumulation in NPC cell lines. Moreover, LMP1 expression was closely related to abnormal β-catenin expression in NPC tissues by immunohistochemistry. LMP1 may be involved in nasopharyngeal carcinogenesis via β-catenin signaling pathway.
authors.affiliation: Department of Pathology, The First Affiliated Hospital and Zhongshan School of Medicine, Sun Yat-sen University, 58, Zhongshan Road II, Guangzhou, 510080, China.
authors.forename: Shuyuan
authors.initials: S
authors.lastname: You
authors.forename: Fenfen
authors.initials: F
authors.lastname: Zhang
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Meng
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Liu
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Liang
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.forename: Wancai
authors.initials: W
authors.lastname: Yang
authors.forename: Anjia
authors.initials: A
authors.lastname: Han
mesh_terms: D021581:Active Transport, Cell Nucleus; D015153:Blotting, Western; D002277:Carcinoma; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D014158:Transcription, Genetic; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D051176:beta Catenin