title: Whole mount RNA in situ hybridization on zebrafish embryos: mounting.
abstract: INTRODUCTIONThis protocol describes the mounting of zebrafish embryos for observation after whole-mount in situ hybridization. Embryos can be mounted in glycerol or dehydrated and mounted in Permount, with or without the yolk. Glycerol causes less shrinkage, but is not as effective a clearing agent. Embryos mounted on the yolk should be stored in the dark and photographed immediately, because the yolks darken rapidly.
authors.affiliation: Division of Basic Science, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Moens