title: Application of micro-thin-layer chromatography as a simple fractionation tool for fast screening of raw extracts derived from complex biological, pharmaceutical and environmental samples.
abstract: The main goal of present paper is to demonstrate the separation and detection capability of micro-TLC technique involving simple one step liquid extraction protocols of complex materials without multi-steps sample pre-purification. In the present studies target components (cyanobacteria pigments, lipids and fullerenes) were isolated from heavy loading complex matrices including spirulina dried cells, birds' feathers and fatty oils as well as soot samples derived from biomass fuel and fossils-fired home heating systems. In each case isocratic separation protocol involving less that 1 mL of one component or binary mixture mobile phases can be completed within time of 5-8 min. Sensitive detection of components of interest was performed via fluorescence or staining techniques using iodine or phosphomolybdic acid. Described methodology can be applied for fast fractionation or screening of whole range of target substances as well as chemo-taxonomic studies and fingerprinting of complex mixtures, which are present in raw biological or environmental samples.
authors.affiliation: Section of Toxicology and Bioanalytics, Department of Civil and Environmental Engineering, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland. pawel_k_z@hotmail.com
authors.forename: Paweł K
authors.initials: PK
authors.lastname: Zarzycki
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Ślączka
authors.forename: Magdalena B
authors.initials: MB
authors.lastname: Zarzycka
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Włodarczyk
authors.forename: Michał J
authors.initials: MJ
authors.lastname: Baran
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods*; D045424:Complex Mixtures / Q000032:analysis*; D004784:Environmental Monitoring; D037741:Fullerenes / Q000032:analysis; D007455:Iodine / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D008832:Microchemistry / Q000295:instrumentation; D008982:Molybdenum / Q000737:chemistry; D010756:Phosphoric Acids / Q000737:chemistry; D053260:Soot / Q000032:analysis; D053545:Spirulina / Q000737:chemistry; D013696:Temperature
chemical_list: D045424:Complex Mixtures; D037741:Fullerenes; D008055:Lipids; D010756:Phosphoric Acids; D053260:Soot; D008982:Molybdenum; D007455:Iodine; C003125:phosphomolybdic acid