title: Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I.
abstract: Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.
authors.affiliation: Centre for Forensic Science, University of Strathclyde, Glasgow G1 1XW, UK. pthanakiatkrai@gmail.com
authors.forename: Phuvadol
authors.initials: P
authors.lastname: Thanakiatkrai
authors.forename: Lindsey
authors.initials: L
authors.lastname: Welch
mesh_terms: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D044707:Forensic Sciences / Q000379:methods*; D009930:Organic Chemicals; D011804:Quinolines; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I