title: Adhesive cell cultivation on polymer particle having grafted epoxy polymer chain.
abstract: In this study, we synthesized a new cell immobilization support having poly(glycidyl methacrylate) as a graft polymer chain and used this support for cell cultivation. Base polymer particle was synthesized by suspension polymerization and epoxy polymer chain was extended from particle surface on graft polymerization. Produced polymer particles had broad particle size distribution ranging from 20 to 1000 μm and the degree of polymerization of grafted polymer chain was ranged from 500 to 1000. The effects of various factors, such as grafted polymer chain length and its surface density, composition of base polymer network and graft polymer chain, on the cell growth of murine fibroblast cell line (MS-5 cell) on polymer particle were studied. This polymer particle could cultivate not only fibroblast cell line but also epidermal cell line (HeLa cell), osteoblast cell line (MC3T3E1 cell), and chondrocyte cell line (ch-8 cell) on its surface. Growth rate is almost the same as that of cells using poly(styrene) tissue culture dish. To apply this cell cultivation system for examination of cell co-culture, HeLa cell immobilized on 100 μm of polymer particle was successfully co-cultured with MS-5 cell immobilized on 300 μm of polymer particle for four weeks.
authors.affiliation: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. yasuda@chemeng.osakafu-u.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yasuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kunieda
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ono
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ogino
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Hiramoto
authors.forename: Wilhelm Robert
authors.initials: WR
authors.lastname: Glomm
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hirabayashi
authors.forename: Shin
authors.initials: S
authors.lastname: Aizawa
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D004852:Epoxy Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology; D006367:HeLa Cells; D006801:Humans; D008689:Methacrylates / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D010316:Particle Size; D016062:Porosity; D014014:Tissue Adhesives / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D014014:Tissue Adhesives; C007870:glycidyl methacrylate