title: Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells.
abstract: The maintenance of viable and functional islets is critical in successful pancreatic islet transplantation from cadaveric sources. During the isolation procedure, islets are exposed to a number of insults including ischemia, oxidative stress and cytokine injury that cause a reduction in the recovered viable islet mass. A novel approach was designed in which streptozotocin (STZ)-damaged rat pancreatic islets (rPIs) were indirectly cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to maintain survival of the cultured rPIs. The results indicated that islets cocultured with rBM-MSCs secreted an increased level of insulin after 14 days, whereas non-cocultured islets gradually deteriorated and cell death occurred. The cocultivation of rBM-MSCs with islets and STZ-damaged islets showed the expression of IL6 and transforming growth factor-β1 in the culture medium, besides the expression of the antiapoptotic genes (Mapkapk2, Tnip1 and Bcl3), implying the cytoprotective, anti-inflammatory and antiapoptotic effects of rBM-SCs through paracrine actions.
authors.affiliation: Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, Kocaeli, Turkey. ekaraoz@hotmail.com
authors.forename: E
authors.initials: E
authors.lastname: Karaoz
authors.forename: Z S
authors.initials: ZS
authors.lastname: Genç
authors.forename: P Ç
authors.initials: PÇ
authors.lastname: Demircan
authors.forename: A
authors.initials: A
authors.lastname: Aksoy
authors.forename: G
authors.initials: G
authors.lastname: Duruksu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D004230:Dithizone / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013311:Streptozocin / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: D016207:Cytokines; D007328:Insulin; D013311:Streptozocin; D004230:Dithizone