title: Expression of the integrin-linked kinase in a rat kidney model of chronic allograft nephropathy.
abstract: The purpose of this study was to investigate the expression and significance of integrin-linked kinase (ILK) in the pathogenesis of chronic allograft nephropathy (CAN) in rats. For this, kidneys of Fisher (F344) rats were orthotopically transplanted into Lewis (LEW) rats. The animals were evaluated at 4, 8, 12, 16, and 24 weeks post-transplantation for renal function and histopathology. ILK protein expression was determined by Western-blot and immunohistological assays, and mRNA by RT-PCR. Our data show that 24-h urinary protein excretion in CAN rats increased significantly at week 16 as compared with F344/LEW controls. Allografts showed markedly increased mononuclear cells infiltration and presented with severe interstitial fibrosis and tubular atrophy at 16 and 24 weeks. ILK expression (protein/mRNA) was upregulated in rat kidneys with CAN, and the increase became more significant over time after transplantation. ILK expression correlated significantly with 24-h urinary protein excretion, serum creatinine levels, tubulointerstitial mononuclear cells infiltration, smooth muscle cells (SMCs) migration in vascular wall, and interstitial fibrosis. Therefore, it was concluded that ILK overexpression was the key event that involved mononuclear cells infiltration and vascular SMCs migration at early stage, and interstitial fibrosis and allograft nephroangiosclerosis at later stage of CAN pathogenesis in rats.
authors.affiliation: Department of Urology, The Affiliated Xuzhou Hospital of Southeast University, Xuzhou 221000, Jiangsu Province, China.
authors.forename: Conghui
authors.initials: C
authors.lastname: Han
authors.forename: Hequn
authors.initials: H
authors.lastname: Zou
authors.forename: Qingqin
authors.initials: Q
authors.lastname: Li
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Shi
authors.forename: Tianyu
authors.initials: T
authors.lastname: Lv
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Wenying
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D005355:Fibrosis / Q000209:etiology; D015971:Gene Expression Regulation, Enzymologic; D006084:Graft Rejection / Q000150:complications / Q000276:immunology / Q000473:pathology*; D007668:Kidney / Q000201:enzymology* / Q000473:pathology*; D016030:Kidney Transplantation / Q000009:adverse effects; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011507:Proteinuria / Q000209:etiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous; D000099200:Scaffold Protein ILK
chemical_list: D003404:Creatinine; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000099200:Scaffold Protein ILK