title: Fluorescence decrease of conjugated polymers by the catalytic activity of horseradish peroxidase and its application in phenolic compounds detection.
abstract: We report the fluorescence decrease of the water-soluble π-π-conjugated polymer poly(2-methoxy-5-propyloxy sulfonate phenylene vinylene, MPS-PPV) by the catalytic activity of horseradish peroxidase in the presence of H(2)O(2). MPS-PPV acts as a donor substrate in the catalytic cycle of horseradish peroxidase where the electron-deficient enzymatic intermediates compounds I and II can subtract electrons from the polymer leading to its fluorescence decrease. The addition of phenolic drug acetaminophen to the former solution favors the decrease of the polymer fluorescence, which indicates the peroxidase-catalyzed co-oxidation of MPS-PPV and acetaminophen. The encapsulation of horseradish peroxidase within polyacrylamide microgels allows the isolation of intermediates compound I and compound II from the polymer, leading to a fluorescence decrease that is only due to the product of biocatalytic acetaminophen oxidation. This system could be used to develop a new device for phenolic compounds detection.
authors.affiliation: Physical Chemistry Department, School of Industrial Engineering, University of Castilla-La Mancha, Albacete 02071, Spain.
authors.forename: M I
authors.initials: MI
authors.lastname: González-Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Laurenti
authors.forename: J
authors.initials: J
authors.lastname: Rubio-Retama
authors.forename: E
authors.initials: E
authors.lastname: Valero
authors.forename: E
authors.initials: E
authors.lastname: Lopez-Cabarcos
mesh_terms: D000082:Acetaminophen / Q000378:metabolism; D055162:Biocatalysis; D006735:Horseradish Peroxidase / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011108:Polymers; D000082:Acetaminophen; D006735:Horseradish Peroxidase