title: Synthesis, DNA binding, and oxidative cleavage studies of Fe(II) and Co(III) complexes containing bioactive ligands.
abstract: The two complexes containing bioactive ligands of the type and [Fe(L)] (PF(6))(2) (1) (where L = [1-{[2-{[2-hydroxynaphthalen-1-yl)methylidine]amino}phenyl)imino] methyl}naphthalene-2-ol]) and [Co(L(1)L(2))] (PF(6))(3) (2) (where L(1)L(2) = mixed ligand of 2-seleno-4-methylquinoline and 1,10-phenanthroline in the ratio 1:2, respectively) were synthesized and structurally characterized. The DNA binding property of the complexes with calf thymus DNA has been investigated using absorption spectra, viscosity measurements, and thermal denaturation experiments. Intrinsic binding constant K(b) has been estimated at room temperature. The absorption spectral studies indicate that the complexes intercalate between the base pairs of the CT-DNA tightly with intrinsic DNA binding constant of 2.8 × 10(5) M(-1) for (1) and 4.8 × 10(5) M(-1) for (2) in 5 mM Tris-HCl/50 mM NaCl buffer at pH 7.2, respectively. The oxidative cleavage activity of (1) and (2) were studied by using gel electrophoresis and the results show that complexes have potent nuclease activity.
authors.affiliation: Department of Biotechnology, Acharya Institute of Technology, Bangalore, Karnataka, India.
authors.forename: B
authors.initials: B
authors.lastname: Sreekanth
authors.forename: G
authors.initials: G
authors.lastname: Krishnamurthy
authors.forename: H S Bhojya
authors.initials: HS
authors.lastname: Naik
authors.forename: T K
authors.initials: TK
authors.lastname: Vishnuvardhan
authors.forename: B
authors.initials: B
authors.lastname: Vinaykumar
authors.forename: N
authors.initials: N
authors.lastname: Sharath
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D008024:Ligands; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D013696:Temperature
chemical_list: D008024:Ligands; D009942:Organometallic Compounds; D003035:Cobalt; D004247:DNA; C427203:calf thymus DNA; D007501:Iron