title: CXCL8 and CCL5 expression in synovial fluid and blood serum in patients with osteoarthritis of the knee.
abstract: Chemokines proved able to induce release of enzymes relevant in cartilage damage. The present study addressed the levels of CXCL8 and CCL5 and the potential role of these chemokines in predicting the morphological changes in the course of osteoarthritis (OA). Synovial fluid (SF) and blood serum were obtained from 20 patients undergoing knee replacement surgery because of OA. For comparison, samples were also obtained from another 20 patients during diagnostic or therapeutic arthroscopy performed because of knee injury. The samples were analyzed for CXCL8 and CCL5 using enzyme-linked immunosorbent assay. SF from the group with OA showed significantly (p = 0.024) increased levels of CXCL8 when compared with the group after knee injury. We have not demonstrated any significant correlation between chemokine expression and clinical or radiological signs of OA. Mediators of inflammation are the potential predicting factors of OA, however, with respect to examined chemokines development of a diagnostic test can be limited by the low serum concentration and lack of correlation with clinical and radiological signs of the disease.
authors.affiliation: Department of Orthopedic and Trauma Surgery, Silesian Medical University, Ziołowa 45/47, Katowice, Poland. adampierzchala@o2.pl
authors.forename: Adam W
authors.initials: AW
authors.lastname: Pierzchala
authors.forename: Damian J
authors.initials: DJ
authors.lastname: Kusz
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Hajduk
mesh_terms: D000328:Adult; D000368:Aged; D019645:Arthroplasty, Replacement, Knee; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D018946:Chemokine CCL5 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000097:blood / Q000000981:diagnostic imaging / Q000276:immunology* / Q000601:surgery; D011859:Radiography; D013582:Synovial Fluid / Q000276:immunology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C483024:CCL5 protein, human; C508606:CXCL8 protein, human; D018946:Chemokine CCL5; D018836:Inflammation Mediators; D016209:Interleukin-8