title: Accuracy of automated and manual systems for susceptibility testing of Pseudomonas aeruginosa to piperacillin and piperacillin-tazobactam.
abstract: This study aimed to evaluate the accuracy of routine systems (Vitek2 cards AST-N022 and AST-N026; Kirby Bauer; Etest) for susceptibility testing of Pseudomonas aeruginosa to piperacillin and piperacillin-tazobactam. Vitek2 (card AST-N022) showed the worst performance; the other three methods (Vitek2 card AST-N026, Kirby-Bauer and E-test) performed comparably but never fulfilled the minimal standard proposed by FDA.
authors.affiliation: Agenzia Sanitaria e Sociale Regionale Emilia-Romagna, Bologna, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Gagliotti
authors.forename: Mario
authors.initials: M
authors.lastname: Sarti
authors.forename: Carla
authors.initials: C
authors.lastname: Sabia
authors.forename: Raffaele
authors.initials: R
authors.lastname: Gargiulo
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.forename: Carmelina
authors.initials: C
authors.lastname: Carillo
authors.forename: Carla
authors.initials: C
authors.lastname: Cassani
authors.forename: Antonio Paolo
authors.initials: AP
authors.lastname: Cipolloni
authors.forename: Federica
authors.initials: F
authors.lastname: Pedna
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Rossi
authors.forename: Silvia Storchi
authors.initials: SS
authors.lastname: Incerti
authors.forename: Giovanna
authors.initials: G
authors.lastname: Testa
authors.forename: Claudia
authors.initials: C
authors.lastname: Venturelli
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Moro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods / Q000592:standards; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology*; D000077725:Piperacillin, Tazobactam Drug Combination; D011237:Predictive Value of Tests; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D000077725:Piperacillin, Tazobactam Drug Combination; D010397:Penicillanic Acid; D010878:Piperacillin