title: Cytokine profiles in aqueous humor of patients with different clinical entities of endogenous uveitis.
abstract: We assayed aqueous humor (AH) samples from patients with Behçet's disease (BD), Vogt-Koyanagi-Harada (VKH) disease, and HLA-B27-associated uveitis and control patients for the proinflammatory cytokines IL-15, IL-17, interferon-γ and tumor necrosis factor-α and the immunosuppressive cytokine IL-10. Cytokine levels were significantly higher in the three disease groups than in controls. In patients with similar disease activity, levels of IL-15 and IFN-γ were significantly higher in BD patients than in VKH and HLA-B27-associated uveitis groups. Logistic regression identified a significant negative correlation between BD and high levels of IL-10 and a significant positive correlation between VKH disease and high levels of IL-10. The proinflammatory cytokines versus IL-10 ratios were significantly higher in BD compared with other groups. These data suggest that both T helper (Th) 1 and Th17 cells are involved in endogenous uveitis immunopathogenesis. BD is characterized by extensive Th1 polarization, severe proinflammatory conditions and a low immunosuppressive status.
authors.affiliation: Department of Ophthalmology, King Abdulaziz University Hospital, College of Medicine, King Saud University, Old Airport Road, PO Box 245, Riyadh 11411, Saudi Arabia. abuasrar@KSU.edu.sa
authors.forename: Ahmed M Abu
authors.initials: AM
authors.lastname: El-Asrar
authors.forename: Sofie
authors.initials: S
authors.lastname: Struyf
authors.forename: Dustan
authors.initials: D
authors.lastname: Kangave
authors.forename: Saleh S
authors.initials: SS
authors.lastname: Al-Obeidan
authors.forename: Ghislain
authors.initials: G
authors.lastname: Opdenakker
authors.forename: Karel
authors.initials: K
authors.lastname: Geboes
authors.forename: Jo
authors.initials: J
authors.lastname: Van Damme
mesh_terms: D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D000328:Adult; D001082:Aqueous Humor / Q000187:drug effects / Q000378:metabolism*; D001528:Behcet Syndrome / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D015796:HLA-B27 Antigen / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D019409:Interleukin-15 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014605:Uveitis / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014607:Uveomeningoencephalitic Syndrome / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D000305:Adrenal Cortex Hormones; D016207:Cytokines; D015796:HLA-B27 Antigen; C508609:IL10 protein, human; D019409:Interleukin-15; D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma; D016572:Cyclosporine