title: Peripheral nerve regeneration using autologous porcine skin-derived mesenchymal stem cells.
abstract: Porcine skin-derived mesenchymal stem cells (pSMSCs) were evaluated on their biological MSC characterizations and differentiation into mesenchymal lineages, along with in vitro and in vivo neural inductions. Isolated pSMSCs showed plate-adherent growth, expression of various MSC-marker proteins and transcriptional factors, and differentiation potential into mesenchymal lineages. Neuron-like cell morphology and various neural markers were highly detected at 6 h and 24 h after in vitro neural induction of pSMSCs, but their neuron-like characteristics disappeared as induction time extended to 48 and 72 h. To evaluate the in vivo peripheral nerve regeneration potential of pSMSCs, a total of 5 × 10(6) autologous pSMSCs labelled with tracking dye, supplemented with fibrin glue scaffold and collagen tubulization, were transplanted into the peripheral nerve defected miniature pigs. At 2 and 4 weeks after cell transplantation, well-preserved transplanted cells and remarkable in vivo nerve regeneration, including histologically complete nerve bundles, were observed in the regenerated nerve tissues. Moreover, S-100 protein and p75 nerve growth factor receptor were more highly detected in regenerated nerve fibres compared to non-cell grafted control fibres. These results suggest that autologous pSMSCs transplanted with fibrin glue scaffold can induce prominent nerve regeneration in porcine peripheral nerve defect sites.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Bong-Wook
authors.initials: BW
authors.lastname: Park
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Kang
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Kang
authors.forename: June-Ho
authors.initials: JH
authors.lastname: Byun
authors.forename: Jong-Sil
authors.initials: JS
authors.lastname: Lee
authors.forename: Geun-Ho
authors.initials: GH
authors.lastname: Maeng
authors.forename: Gyu-Jin
authors.initials: GJ
authors.lastname: Rho
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation; D058948:Cell Tracking; D005786:Gene Expression Regulation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010525:Peripheral Nerves / Q000473:pathology / Q000502:physiology*; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D034421:Sus scrofa; D013552:Swine; D013556:Swine, Miniature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014182:Transplantation, Autologous
chemical_list: D020800:Receptor, Nerve Growth Factor; D014157:Transcription Factors