title: Anti-tumor effect of adenoviral vector-mediated p53 gene transfer on the growth of canine osteosarcoma xenografts in nude mice.
abstract: We evaluated the anti-tumor effect of adenoviral vector-mediated p53 gene therapy on the growth of canine osteosarcoma xenografts formed in nude mice. Nude mice were subcutaneously transplanted with cells of 2 P53 mutant canine osteosarcoma cell lines, POS and CHOS. The osteosarcoma xenografts were injected with either an adenoviral vector that expresses canine wild-type P53 (AxCA-cp53) or LacZ (AxCA-LacZ). Tumor growth was significantly inhibited in the xenografts injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS during the observation period of 27 days. An increase of the amount of p21(WAF1/CDKN1A) mRNA, and the number of apoptotic cells was shown in the tumors injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS. The present study revealed that the adenoviral vector-mediated p53 gene transfer had an anti-tumor effect in canine osteosarcoma xenografts formed in nude mice.
authors.affiliation: Department of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kanaya
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yazawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D004285:Dogs; D005260:Female; D016158:Genes, p53; D015316:Genetic Therapy / Q000379:methods* / Q000592:standards; D005822:Genetic Vectors / Q000235:genetics; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012516:Osteosarcoma / Q000473:pathology / Q000628:therapy / Q000662:veterinary*; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D023041:Xenograft Model Antitumor Assays
chemical_list: D012334:RNA, Neoplasm