title: Metabolite profiling and antioxidant activity of Prunus padus L. flowers and leaves.
abstract: Six phenolics were obtained from the leaves of Prunus padus by activity-guided isolation: isorhamnetin 3-O-β-xylopyranosyl-(1 → 2)-β-galactopyranoside (1), astragalin (2), hyperoside (3), quercetin 3-O-β-xylopyranosyl-(1 → 2)-β-galactopyranoside (4), quercetin 3-O-β-xylopyranosyl-(1 → 2)-β-glucopyranoside (5) and chlorogenic acid (6). The antioxidant potential of 70% methanolic extracts from the flowers and leaves collected over the growing season was evaluated using the 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging and 2,2′-azobis-(2-amidinopropane) dihydrochloride (AAPH)-induced linoleic acid (LA) peroxidation tests in relation to the contents of the isolates 1-6, total phenolics, total proanthocyanidins and total quercetin. The IC₅₀ values were expressed in gram dry weight per gram of DPPH or LA, respectively, and were in the range of 1.42-2.42 for the DPPH test and 1.78-4.92 for the LA peroxidation, with superior activity found for the flowers and the autumn leaves. Significant linear correlation of these values to the sum of proanthocyanidins and compounds 1-6 (R² > 0.87) showed that the listed phenolics are synergists of the tested activity.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Łódź, ul. Muszyńskiego 1, 90-151 Łódź, Poland. monika.olszewska@umed.lodz.pl
authors.forename: Monika A
authors.initials: MA
authors.lastname: Olszewska
authors.forename: Anna
authors.initials: A
authors.lastname: Kwapisz
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds; D002726:Chlorogenic Acid; D002851:Chromatography, High Pressure Liquid; D035264:Flowers / Q000378:metabolism*; D016166:Free Radical Scavengers; D006027:Glycosides; D020128:Inhibitory Concentration 50; D044949:Kaempferols; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010636:Phenols / Q000302:isolation & purification / Q000378:metabolism*; D010851:Picrates; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D027861:Prunus / Q000378:metabolism*; D011794:Quercetin / Q000031:analogs & derivatives; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006027:Glycosides; D044949:Kaempferols; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D002726:Chlorogenic Acid; C071751:quercetin 3-sambubioside; C021304:hyperoside; D011794:Quercetin; C001579:astragalin; C004931:1,1-diphenyl-2-picrylhydrazyl