title: Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process.
abstract: Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific F(1)s. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticum aestivum L.) and molecular markers were used to demonstrate the SynDH method.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
authors.forename: Lianquan
authors.initials: L
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Luo
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Hao
authors.forename: Baolong
authors.initials: B
authors.lastname: Liu
authors.forename: Zehong
authors.initials: Z
authors.lastname: Yan
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Huaigang
authors.initials: H
authors.lastname: Zhang
authors.forename: Youliang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Dengcai
authors.initials: D
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yen
mesh_terms: D001947:Breeding / Q000379:methods*; D005983:Glutens / Q000032:analysis; D006238:Haploidy; D006720:Homozygote; D006824:Hybridization, Genetic; D057891:Tetraploidy; D014908:Triticum / Q000235:genetics*
chemical_list: D005983:Glutens; C037302:glutenin