title: In vitro transcription using Drosophila nuclear extracts.
abstract: INTRODUCTIONDrosophila nuclear extracts are often used in gene expression studies. This protocol describes RNA polymerase II transcription in vitro using a Drosophila embryonic nuclear extract called soluble nuclear fraction (SNF). Transcription and RNA purification are followed by detection of the transcripts by primer extension with reverse transcriptase and a labeled oligonucleotide.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Pazin