title: Nano-engineered flavin-dependent glucose dehydrogenase/gold nanoparticle-modified electrodes for glucose sensing and biofuel cell applications.
abstract: A three-dimensional composite consisting of the oxygen-insensitive flavin-dependent glucose dehydrogenase, GDH, and Au nanoparticles (NPs) is assembled on a Au surface using an electropolymerization process. The bis-aniline-cross-linked GDH/Au NPs composite reveals effective electrical contact with the electrode (ket=1100 s(-1)), and the effective bioelectrocatalyzed oxidation is driven by the enzyme/NPs matrix. The GDH/Au NPs-functionalized electrode is implemented as an amperometric glucose sensor, and it reveals superior functions when compared to an analogous glucose oxidase/Au NPs system. The O2-insensitive GDH/Au NPs composite electrode was further used as an anode in a membraneless glucose/O2 biofuel cell. The cathode in this system was composed of bilirubin oxidase cross-linked onto a carbon nanotube-modified glassy carbon electrode. The power output of the cell was 32 μW cm(-2).
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Omer
authors.initials: O
authors.lastname: Yehezkeli
authors.forename: Ran
authors.initials: R
authors.lastname: Tel-Vered
authors.forename: Sara
authors.initials: S
authors.lastname: Raichlin
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005415:Flavins / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D005415:Flavins; D006046:Gold; D042843:Glucose 1-Dehydrogenase; D005947:Glucose