title: Immunolocalization of pathogen effectors.
abstract: The use of polyclonal antibodies enables the detection of proteins on a cellular and even subcellular level. Immunolocalization can be used on all pathosystems even if one or both partners of the interaction are unamenable to molecular tools like transformation. This chapter provides detailed information about how to obtain high quality antibodies, how to prepare samples, and finally how to detect the proteins. Methods for light and electron microscopy are presented.
authors.affiliation: The Sainsbury Laboratory, John Innes Centre, Norwich, UK.
authors.forename: Eric
authors.initials: E
authors.lastname: Kemen
authors.forename: Kurt
authors.initials: K
authors.lastname: Mendgen
authors.forename: Ralf T
authors.initials: RT
authors.lastname: Voegele
mesh_terms: D000818:Animals; D000906:Antibodies; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D009868:Oomycetes / Q000648:ultrastructure; D010944:Plants / Q000382:microbiology
chemical_list: D000906:Antibodies