title: Norepinephrine enhances radiosensitivity in rat ileal epithelial cells.
abstract: We previously reported that the apoptosis index in jejunal crypt cells after X irradiation was greater in spontaneously hypertensive rats than in Wistar-Kyoto rats. Moreover, these same cells showed a suppression of apoptosis when reserpine was administered to induce sympathetic dysfunction in spontaneously hypertensive rats or Wistar-Kyoto rats. Whether the hyperfunction of the sympathetic nervous system is involved in the high susceptibility of the jejunal crypt cells to radiation-induced apoptosis was the subject of this study. The effect of norepinephrine (NE) on cell survival was examined using the colony formation assay after X-ray irradiation of rat ileal epithelial cells (IEC-18). The addition of 1 µM NE decreased the surviving fraction of cells irradiated with 6 Gy from 37% to 8%. The radiosensitivity of IEC-18 cells was enhanced by the addition of 1 µM of NE. The irradiation and treatment with NE also resulted in an increased cellular apoptotic rate. These results showing enhanced radiosensitivity of rat ileal epithelial cells by NE suggest that NE may be one of the factors which aggravate acute radiation injury in the intestine.
authors.affiliation: Department of Tumor and Diagnostic Pathology, Nagasaki University Graduate School of Biomedical Sciences. mutsumi@nagasaki-u.ac.jp
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Matsuu-Matsuyama
authors.forename: Kumio
authors.initials: K
authors.lastname: Okaichi
authors.forename: Kazuko
authors.initials: K
authors.lastname: Shichijo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nakayama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakashima
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekine
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D007082:Ileum / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D009638:Norepinephrine / Q000494:pharmacology*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000187:drug effects / Q000502:physiology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D011838:Radiation-Sensitizing Agents; D009638:Norepinephrine