title: Preservation methods of allografts and their (lack of) influence on clinical results in partial thickness burns.
abstract: Allografts, cadaver skin and amnion membrane are considered the golden standard in the management of partial thickness burns. However, debate on whether the tissue needs to be viable is on-going, since many believe that viable grafts result in better healing. The objective of this literature survey was to analyse the evidence on the method of preservation of allografts (cadaver skin or amnion membrane, glycerol, cryopreservation, lyophilisation) having a clinical impact on the healing of partial thickness burns. The survey focussed on preservation techniques and clinical outcomes (reepithelialisation) in partial thickness burns, as well as on differences in viability, immunogenicity and antimicrobial properties of the preservation methods. Most studies on allograft treatment of partial thickness burns are observational, with only one study of a (historical) comparative nature. A true meta-analysis was not performed and the results of this survey are observational in nature as well: they indicate that there is no evidence that viability of the graft influences healing outcomes. Thus, instead of viability, other aspects, such as intrinsic antimicrobial safety of the preservation method and cost should be the primary criteria for the choice of preservation method to be used for allografts.
authors.affiliation: President Hermans Consulting Inc., 3 Lotus Place, Newtown, PA 18940, USA. Hermansconsulting@comcast.net
authors.forename: Michel H E
authors.initials: MH
authors.lastname: Hermans
mesh_terms: D002056:Burns / Q000601:surgery*; D015925:Cryopreservation; D005612:Freeze Drying; D005990:Glycerol; D006085:Graft Survival; D006801:Humans; D012867:Skin / Q000276:immunology / Q000382:microbiology; D016038:Skin Transplantation / Q000276:immunology; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D005990:Glycerol