title: Permeation modulating properties of natural polymers--effect of molecular weight and mucus.
abstract: The permeation modulating effects of 5 natural polymers; low-ester, amidated and high-ester pectin, as well as hyaluronic acid and chitosan were tested at two different molecular weights each. The model membrane was methotrexate treated HT29 cells grown for 2 or 3 weeks, respectively, thereby differing in the amount of goblet cells and hence mucus. The pectins decreased the permeation of the paracellular marker carboxyfluorescein. Free acid groups and a high molecular weight increased this membrane protective effect. Chitosan displayed pronounced and hyaluronic acid modest permeation enhancing properties. In this case, a low molecular weight accentuated the effect. In all cases, the permeation modulating properties were reduced by mucus.
authors.affiliation: Institute of Physics and Chemistry, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. ellen@ifk.sdu.dk
authors.forename: Ellen
authors.initials: E
authors.lastname: Hagesaether
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D005079:Excipients / Q000737:chemistry; D005452:Fluoresceins / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D020397:Goblet Cells / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008727:Methotrexate / Q000737:chemistry; D008970:Molecular Weight; D009093:Mucus / Q000378:metabolism; D010368:Pectins / Q000737:chemistry; D010539:Permeability; D011108:Polymers / Q000737:chemistry*
chemical_list: D005079:Excipients; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011108:Polymers; C024098:6-carboxyfluorescein; D010368:Pectins; D006820:Hyaluronic Acid; D048271:Chitosan; D008727:Methotrexate