title: Human cytomegalovirus UL97 D605E polymorphism has a high prevalence in immunocompetent Japanese infants and children.
abstract: There is no existing data on UL97 mutation in human cytomegalovirus (HCMV) isolates obtained from individuals who have never been exposed to ganciclovir (GCV). UL97 codons 439 to 645 from 61 CMV isolates from 61 immunocompetent Japanese infants and children were sequenced directly. No known GCV resistance mutations were found, meaning that the UL97 mutation had resulted from the use of GCV. On the other hand, a mutation at codon 605 (D to E) was frequently identified (56/61: 91.8%). This could be a genetic marker for HCMV in East Asian counties, because of its low prevalence in the strains of HCMV circulating in Western countries.
authors.affiliation: Department of Pediatrics, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan. ktanaka@sapmed.ac.jp
authors.forename: Kaori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Hori
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yoto
authors.forename: Naoki
authors.initials: N
authors.lastname: Hatakeyama
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsutsumi
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use; D044466:Asian People / Q000235:genetics*; D002648:Child; D003062:Codon; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000821:virology; D024882:Drug Resistance, Viral; D015774:Ganciclovir / Q000627:therapeutic use; D006801:Humans; D007121:Immunocompetence; D007223:Infant; D017720:Molecular Epidemiology; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D000998:Antiviral Agents; D003062:Codon; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase; D015774:Ganciclovir