title: An electrochemiluminescence sensor for determination of durabolin based on CdTe QD films by layer-by-layer self-assembly.
abstract: This work reported for the first time the use of flow injection electrochemiluminescence (FI-ECL) sensor for the determination of durabolin in an aqueous system based on CdTe quantum dot (QD) films. Aqueous CdTe colloidal solutions were prepared using thioglycolic acid as a capping agent. Zetasizer Nano ZS (Malvern, UK) was employed to characterize the size of CdTe QDs. The UV-vis and photoluminescence spectra of samples were systematically characterized. Indium tin oxide (ITO) slide glass was modified with CdTe QDs by layer-by-layer self-assembly. CdTe QD films were packed into a homemade cell and used as a recognizer of the FI-ECL sensor to determine durabolin. The intensive anodic ECL emission was obtained at a starting potential of +1.3 V (vs. Ag/AgCl) in a carbonate bicarbonate buffer solution with a pH of 9.93 at an ITO electrode. The ECL intensity was correlated linearly with the concentration of durabolin over the range of 1.0 × 10(-8)-1.0 × 10(-5) g mL(-1), and the detection limit was 2.5 × 10(-9) g mL(-1). The relative standard deviation for the determination of 1.0 × 10(-6) g mL(-1) durabolin was 1.04% (n = 11). This simple and sensitive sensor revealed good reproducibility for ECL analysis. As a result, the new FI-ECL sensor had been successfully applied to the determination of durabolin in food samples. This strategy could be easily realized and opened new avenues for the applications of QDs in ECL biosensing.
authors.affiliation: Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, China. wanfw1984@hotmail.com
authors.forename: Fuwei
authors.initials: F
authors.lastname: Wan
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
mesh_terms: D045930:Anabolic Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000032:analysis; D045663:Quantum Dots; D013691:Tellurium / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D045930:Anabolic Agents; D019187:Cadmium Compounds; D014867:Water; D009277:Nandrolone; C008580:nandrolone phenpropionate; D013691:Tellurium; C028337:cadmium telluride