title: Levels of phosphorylated axonal neurofilament subunit H (pNfH) are increased in acute ischemic stroke.
abstract: For the study of stroke outcomes, there is the need for measurements of severity of stroke damage. Phosphorylated neurofilament heavy protein (pNfH) levels are elevated in axonal injury. We have measured levels of pNfH in stroke and correlated these levels with measures of stroke severity. Blood samples were collected from 54 ischaemic stroke patients at day 1, week 1 (days 7-10) and weeks 3-6, and an ELISA was used to measure pNfH levels in each patient at each time-point. Serum pNfH levels were significantly elevated in stroke patients compared to healthy controls. The levels were low at day 1, higher at day 7 and reached a peak at week 3, the latest day that we assessed. Significant associations were found between the pNfH levels at week 3 and early and stroke severity, size and outcome. Blood pNfH levels that reflect the severity of ischaemic stroke, are correlated with outcome and rise during the weeks after stroke. This may be a useful measure of tissue damage in stroke.
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Singh
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: R
authors.initials: R
authors.lastname: Hull
authors.forename: S
authors.initials: S
authors.lastname: Read
authors.forename: J
authors.initials: J
authors.lastname: O'Sullivan
authors.forename: R D
authors.initials: RD
authors.lastname: Henderson
authors.forename: S
authors.initials: S
authors.lastname: Rose
authors.forename: J M
authors.initials: JM
authors.lastname: Greer
authors.forename: P A
authors.initials: PA
authors.lastname: McCombe
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001369:Axons / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002545:Brain Ischemia / Q000097:blood* / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000097:blood* / Q000378:metabolism; D010766:Phosphorylation / Q000502:physiology; D012720:Severity of Illness Index; D020521:Stroke / Q000097:blood* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016900:Neurofilament Proteins; C051774:neurofilament protein H