title: [Quantitative determination of six coumarins in Angelica dahurica var. formosana by RRLC-UV].
abstract: OBJECTIVE
  To establish a RRLC-UV method for simultaneous determination of oxypeucedanin hydrate, bergapten, oxypeucedanin, imperatorin, cnidilin and oxypeucedanin in Angelica dahurica var. formosana.
  
  
  METHODS
  The analysis was carried out on a Agilent Zorbax SB-C, (4.6 mm x 100 mm, 1.8 microm) column eluted with the mobile phases of methanol (A)-water (B) in gradient elution. The flow rate was 1.0 mL x min(-1), and the UV detection wavelength was set at 310 nm.
  
  
  RESULTS
  All calibration curves of six coumarins showed good linearity regression within test ranges (r > 0.9998), and the overrall recoveries were in the range of 99.42%-101.4%, with RSD less than 2.4%.
  
  
  CONCLUSIONS
  The method is simple, sensitive, reliable and reproducible which can be used for the quality study of Angelica dahurica var. formnosana.
authors.affiliation: Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. dengrui18@gmail.com
authors.forename: Rui
authors.initials: R
authors.lastname: Deng
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Weizao
authors.initials: W
authors.lastname: Luo
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Haijie
authors.initials: H
authors.lastname: Zheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003374:Coumarins / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D003374:Coumarins; D010936:Plant Extracts