title: Tepidibacter mesophilus sp. nov., a mesophilic fermentative anaerobe isolated from soil polluted by crude oil, and emended description of the genus Tepidibacter.
abstract: A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1(T), was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1-1.6 µm wide and 2.3-4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10-40 °C and pH 6.0-8.9; optimal growth occurred at 28-32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1% (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9% (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, D-glucose, D-fructose, D-xylose, D-ribose, D-galactose, maltose, L-rhamnose, trehalose, L-valine, DL-alanine plus L-proline and DL-alanine plus L-glycine. The main products of glucose fermentation were ethanol and acetate. iso-C(15:0), iso-C(14:0), C(16:0) and iso-C(13:0) were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Tepidibacter, showing 94.7 and 94.1% similarity to the type strains of Tepidibacter formicigenes and Tepidibacter thalassicus, respectively. The genomic DNA G+C content of strain B1(T) was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1(T) is suggested to represent a novel species of the genus Tepidibacter, for which the name Tepidibacter mesophilus sp. nov. is proposed. The type strain is B1(T) (=CGMCC 1.5148(T) =JCM 16806(T)).
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Tan
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xiao-Yue
authors.initials: XY
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu
mesh_terms: D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D005407:Flagella / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D012492:Salts; D012989:Soil Pollutants