title: Preparation of immobilized enzyme microcolumns.
abstract: INTRODUCTIONPeptide mapping by MALDI-TOF-MS has emerged as a powerful proteomics tool for identifying and characterizing proteins. One of the key steps in this method involves the proteolytic cleavage of proteins, followed by MS analysis of the generated peptides. A limitation of the method, especially for high-throughput proteomics, is the speed and efficiency of proteolytic cleavage. In this protocol, a method for rapid tryptic digestion using immobilized enzyme microcolumns is described.
authors.forename: Martin R
authors.initials: MR
authors.lastname: Larsen