title: Down-regulation of miR-23b may contribute to activation of the TGF-β1/Smad3 signalling pathway during the termination stage of liver regeneration.
abstract: MicroRNAs (miRNAs) are known to play important roles in liver regeneration, although the role of miRNAs associated with the termination of liver regeneration is not as well studied. Here we reported the down-regulation of miR-23b in the termination stage of liver regeneration in rats. In addition, Smad3 was identified as a target of miR-23b during liver regeneration. Up-regulation of miR-23b promoted BRL-3A cell proliferation and partially inhibited transforming growth factor (TGF)-β1-induced apoptosis. Furthermore, TGF-β1 transcriptionally inhibited miR-23b expression. We conclude that down-regulation of miR-23b may contribute to activation of the TGF-β1/Smad3 signalling pathway during the termination stage of liver regeneration.
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yuan
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Dong
authors.forename: Duo
authors.initials: D
authors.lastname: Shi
authors.forename: Yunheng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Mingyong
authors.initials: M
authors.lastname: Miao
authors.forename: Binghua
authors.initials: B
authors.lastname: Jiao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002460:Cell Line; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D008115:Liver Regeneration; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D051900:Smad3 Protein / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: C552430:MIRN23a microRNA, human; D035683:MicroRNAs; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1