title: Whole-mount in situ hybridization in amphimedon.
abstract: INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.
authors.affiliation: School of Integrative Biology, University of Queensland, Brisbane QLD 4072, Australia.
authors.forename: Claire
authors.initials: C
authors.lastname: Larroux
authors.forename: Bryony
authors.initials: B
authors.lastname: Fahey
authors.forename: Maja
authors.initials: M
authors.lastname: Adamska
authors.forename: Gemma S
authors.initials: GS
authors.lastname: Richards
authors.forename: Marie
authors.initials: M
authors.lastname: Gauthier
authors.forename: Kathryn
authors.initials: K
authors.lastname: Green
authors.forename: Erica
authors.initials: E
authors.lastname: Lovas
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Degnan