title: [Research on phytoestrogenic effect of formononetin].
abstract: OBJECTIVE
  Research on the phytoestrogenic effect and its possible mechanism of formononetin.
  
  
  METHODS
  To evaluate the estrogenic effect and mechanisms of formononetin through the test of its influence on proliferation and ER subtype expression of T47D cells.
  
  
  RESULTS
  The proliferation rates of T47D cells treated with 1 x 10(-7) -1 x 10(-6) mol x L(-1) formononetin were not increased. On the influence of ICI182, 780, the proliferation rates of T47D cells treated with 1 x 10(-7) 1 x 10(-6) mol x L(-1) formononetin were decreased. Formonenetin could induce the augment of ERalpha expression significantly of T47D.
  
  
  CONCLUSIONS
  Formonenetin has phytoestrogenic effect Formonenetin can not accelerate ER(+) T47D cell proliferation. But the expression level of ERalpha subtype in T47D cells change significantly with certain concentrations of formonenetin.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Piwen
authors.initials: P
authors.lastname: Zhao
authors.forename: Jianzhao
authors.initials: J
authors.lastname: Niu
authors.forename: Jifeng
authors.initials: J
authors.lastname: Wang
authors.forename: Yuankui
authors.initials: Y
authors.lastname: Cao
authors.forename: Qingxiu
authors.initials: Q
authors.lastname: Hao
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D048789:Phytoestrogens / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens; D011960:Receptors, Estrogen; C007768:formononetin