title: Effects of static magnetic fields on nicotinic cholinergic receptor function.
abstract: Ligand-gated ion channel kinetics were studied in mammalian transfected cells encoding adult mouse muscle acetylcholine (ACh) receptors. We measured macroscopic and single-channel currents using the outside-out and cell-attached patch-clamp configurations. Cultured cells were exposed to moderate intensity inhomogeneous static magnetic fields up to 180 mT and measurements were performed for temperatures ranging from 5 to 50 °C. We found no significant changes in ACh-elicited macroscopic or single-channel currents. We observed the expected dependence in current decay constants with temperature, but negligible magnetic field influence on the channel's kinetics.
authors.affiliation: Departamento de Física, Universidad Nacional del Sur, Bahía Blanca, Argentina.
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Tolosa
authors.forename: Cecilia
authors.initials: C
authors.lastname: Bouzat
authors.forename: Walter Rubén
authors.initials: WR
authors.lastname: Cravero
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D058446:Ligand-Gated Ion Channels / Q000378:metabolism; D060526:Magnetic Fields; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology; D018408:Patch-Clamp Techniques; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013696:Temperature
chemical_list: D058446:Ligand-Gated Ion Channels; D011978:Receptors, Nicotinic