title: Trifluoroethanol-induced activity and structural changes in bos taurus copper- and zinc-containing superoxide dismutase.
abstract: Superoxide dismutase (SOD, EC 1.15.1.1) plays an important antioxidant defense role in organisms exposed to oxygen. Copper- and zinc-containing SOD (Cu/Zn-SOD) catalysis and the change in folding behavior of this enzyme in response to inactivators are therefore of interest. We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of a Cu/Zn-SOD from Bos taurus. We found that TFE inactivated the enzyme and disrupted the tertiary and secondary structures of Cu/Zn-SOD. Kinetic studies showed that TFE-induced inactivation of Cu/Zn-SOD follows first-order reaction kinetics and that TFE binding sites are distinct from the copper- and zinc-containing active site. These structural changes occurred prior to enzyme activity loss. A computational docking simulation of Cu/Zn-SOD and TFE (binding energy of Dock 6.3: -11.52 kcal/mol) suggested that THR37, ASP40, and GLU119, which are located near the active site, interact with TFE. Evaluation of the ligand binding kinetics of Cu/Zn-SOD during unfolding in the presence of TFE combined with computational prediction allowed us to gain insight into the inactivation of Cu/Zn-SOD.
authors.affiliation: Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Shi
authors.forename: Yong
authors.initials: Y
authors.lastname: Xia
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Shang-Jun
authors.initials: SJ
authors.lastname: Yin
authors.forename: Yue-Xiu
authors.initials: YX
authors.lastname: Si
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Qian
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Lü
authors.forename: Hai-Meng
authors.initials: HM
authors.lastname: Zhou
authors.forename: D
authors.initials: D
authors.lastname: Park
authors.forename: G
authors.initials: G
authors.lastname: Chng
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.forename: Yong-Doo
authors.initials: YD
authors.lastname: Park
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism*; D014270:Trifluoroethanol / Q000494:pharmacology*
chemical_list: D014270:Trifluoroethanol; D013482:Superoxide Dismutase