title: Mucosal and systemic immune responses of mice to tetanus toxoid coadministered nasally with AFCo1.
abstract: Mucosal immune responses are an early and important line of defense against pathogens. The current understanding of the mucosal immune system allows us to consider the use of nasal immunization for induction of antigen-specific immune responses at the mucosal surface and the systemic compartment. Mucosal adjuvants are key for developing novel mucosal vaccines and represent 1 approach to improving mucosal and systemic immunity. However, few mucosal vaccine adjuvants are currently approved for human use. Neisseria meningitidis B proteoliposome-derived cochleate (AFCo1 - Adjuvant Finlay Cochleate 1) has been demonstrated to be a potent mucosal adjuvant. The present work demonstrates that intranasal immunization of 3 doses of tetanus toxoid (TT) coadministered with AFCo1 in mice promotes high systemic and mucosal responses. The anti-TT IgG serum titers and the mucosal anti-TT IgA in saliva and vaginal wash were significantly higher than TT alone. The analysis of antibody subclasses showed that intranasal administration of AFCo1 + TT induced not only IgG1 but also IgG2a anti-TT antibodies at levels comparable to those obtained with TT vaccine (vax-TET). These data support the fact that AFCo1 is a potent mucosal adjuvant in nasal immunization to a coadministered protein antigen.
authors.affiliation: Immunology Department, Finlay Institute, Calle 17 esquina 198 Rpto Siboney, Havana 11600, Cuba. bromeu@finlay.edu.cu
authors.forename: Belkis
authors.initials: B
authors.lastname: Romeu
authors.forename: Elyzabeth
authors.initials: E
authors.lastname: González
authors.forename: Judith
authors.initials: J
authors.lastname: Del Campo
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Acevedo
authors.forename: Caridad
authors.initials: C
authors.lastname: Zayas
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Valdés
authors.forename: Osmir
authors.initials: O
authors.lastname: Cabrera
authors.forename: Maribel
authors.initials: M
authors.lastname: Cuello
authors.forename: Julio
authors.initials: J
authors.lastname: Balboa
authors.forename: Miriam
authors.initials: M
authors.lastname: Lastre
authors.forename: Oliver
authors.initials: O
authors.lastname: Pérez
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000282:Administration, Intravaginal; D000818:Animals; D000917:Antibody Formation; D005260:Female; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D038541:Neisseria meningitidis, Serogroup B; D011510:Proteolipids / Q000008:administration & dosage / Q000276:immunology; D012463:Saliva / Q000276:immunology; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000276:immunology*; D014621:Vagina / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D011510:Proteolipids; D013745:Tetanus Toxoid; C020485:proteoliposomes