title: A genetic system for RNase E variant-controlled overproduction of ColE1-type plasmid DNA.
abstract: Escherichia coli endoribonuclease RNase E (Rne) regulates replication of ColE1-type plasmids by cleaving RNA I transcripts, which are synthesized from the plasmid and regulate the plasmid replication as antisense RNA. Here, we report the development of a genetic system that efficiently overproduces ColE1-type plasmid DNA when an RNase E variant that confers a hyperactive phenotype in RNA I cleavage is conditionally expressed from chromosome. This genetic system offers a method for isolation of large quantities of pure ColE1-type plasmid DNA, which have been most commonly used as molecular biology and biotechnology tools for research and industrial purposes.
authors.affiliation: School of Biological Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Hayoung
authors.initials: H
authors.lastname: Go
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D015152:Blotting, Northern; D015139:Blotting, Southern; D004261:DNA Replication / Q000502:physiology*; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000096:biosynthesis* / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C042027:RNA I; D012329:RNA, Bacterial; C042833:ROM protein, Bacteria; D004722:Endoribonucleases; C022537:ribonuclease E