title: A multiplex polymerase chain reaction method for rapid pneumococcal serotype determination in childhood empyema.
abstract: Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A (n = 6), 1 (n = 2), 3 (n = 1) and 7F/A (n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema.
authors.affiliation: Service de Microbiologie, Hôpital Robert-Debré (APHP), 75019 Paris, Université Denis Diderot-Paris 7, France.
authors.forename: Naouale
authors.initials: N
authors.lastname: Maataoui
authors.forename: Philippe
authors.initials: P
authors.lastname: Bidet
authors.forename: Catherine
authors.initials: C
authors.lastname: Doit
authors.forename: Agathe
authors.initials: A
authors.lastname: De Lauzanne
authors.forename: Mathie
authors.initials: M
authors.lastname: Lorrot
authors.forename: Patricia
authors.initials: P
authors.lastname: Mariani-Kurkdjian
authors.forename: Albert
authors.initials: A
authors.lastname: Faye
authors.forename: Edouard
authors.initials: E
authors.lastname: Bingen
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D016724:Empyema, Pleural / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010996:Pleural Effusion / Q000382:microbiology; D011008:Pneumococcal Infections / Q000382:microbiology*; D022242:Pneumococcal Vaccines; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000145:classification*
chemical_list: D022242:Pneumococcal Vaccines