title: [Study on the dynamic variation of active components in Geranium carolinianum from different collection time].
abstract: OBJECTIVE
  To determine the contents of the active components, gallic acid and ellagic acid in Geranium carolinianum from different collection time and to define the best collection time for this herb.
  
  
  METHODS
  The contents of gallic acid and ellagic acid in each samples of Geranium carolinianum were determined by HPLC. The HPLC method was performed on a Diamonsil C, 8 column (150 mm x 4.6 mm, 5 microm) with acetonitrile-0.1% H3PO4 as mobile phase. The flow rate was 1.0 mL/min, the detection wavelength was 274 nm and the column temperature was 25 degrees C.
  
  
  RESULTS
  The calibration curve of gallic acid and ellagic acid were linear in the range of 0.075-5.00 microg (r = 0.9995) and 0.05-2.00 microg (r = 0.9995), respectively. The average recovery of gallic acid and ellagic acid were 99.88% (RSD = 1.19%) and 99.08% (RSD = 2.81%), respectively.
  
  
  CONCLUSIONS
  The content of gallic acid and ellagic acid in Geranium carolinianum both began to increase in flowering stage and increased to the maximum value in immature-fruit stage.
authors.affiliation: China Pharmaceutical University, School of Traditional Chinese Pharmacy, Nanjing 211198, China. lifengwen49@hotmail.com
authors.forename: Feng-Wen
authors.initials: FW
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Lan
authors.initials: L
authors.lastname: Yao
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Dong
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Gong
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry; D000998:Antiviral Agents / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis*; D031315:Geranium / Q000737:chemistry* / Q000254:growth & development; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D015203:Reproducibility of Results; D012621:Seasons
chemical_list: D000975:Antioxidants; D000998:Antiviral Agents; D004610:Ellagic Acid; D005707:Gallic Acid