title: Applicability of equine hydroxyapatite collagen (eHAC) bone blocks for lateral augmentation of the alveolar crest. A histological and histomorphometric analysis in rats.
abstract: This study assessed the mechanical characteristics, biocompatibility and osteoconductive properties of an equine hydroxyapatite collagen (eHAC) bone block when applied as a bone substitute for lateral augmentation of rat mandible. 96 rats underwent lateral augmentation of the mandible, using two substitute bone blocks (eHAC or Bio-Oss spongiosa) or autologous bone grafts. Signs of inflammation, amount of bone formation and ingrowth of bone into the bone blocks were assessed at 1 and 3 months. eHAC blocks were mechanically rigid and could be fixed firmly and easily. Bio-Oss spongiosa blocks were brittle and fixation was difficult. eHAC and Bio-Oss spongiosa blocks were biocompatible and induced few or no signs of inflammation. Inflammation prevalence between the groups was not statistically different. Bone formation and bone growth into the blocks was significantly higher in eHAC than Bio-Oss spongiosa blocks, but lower than in autologous bone grafts (after 1 and 3 months). Regression analysis showed that the autologous bone graft predicted new bone formation at both time points. The eHAC block was only a predictor at 1 month; a trend was found at 3 months. The application of biodegradable membranes was not related to more bone ingrowth.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Center Groningen, University of Groningen, The Netherlands. jerry.zecha@cze.nl
authors.forename: P J
authors.initials: PJ
authors.lastname: Zecha
authors.forename: J
authors.initials: J
authors.lastname: Schortinghuis
authors.forename: J E
authors.initials: JE
authors.lastname: van der Wal
authors.forename: H
authors.initials: H
authors.lastname: Nagursky
authors.forename: K C
authors.initials: KC
authors.lastname: van den Broek
authors.forename: S
authors.initials: S
authors.lastname: Sauerbier
authors.forename: A
authors.initials: A
authors.lastname: Vissink
authors.forename: G M
authors.initials: GM
authors.lastname: Raghoebar
mesh_terms: D020341:Absorbable Implants; D000540:Alveolar Ridge Augmentation / Q000295:instrumentation / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001696:Biomechanical Phenomena; D001857:Bone Matrix / Q000637:transplantation; D001863:Bone Screws; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods; D003094:Collagen / Q000627:therapeutic use*; D017886:Durapatite / Q000627:therapeutic use*; D006736:Horses; D007249:Inflammation; D008297:Male; D008334:Mandible / Q000473:pathology / Q000601:surgery*; D008567:Membranes, Artificial; D008903:Minerals / Q000627:therapeutic use; D010012:Osteogenesis / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous
chemical_list: C106416:Bio-Gide; C077540:Bio-Oss; D001672:Biocompatible Materials; D018786:Bone Substitutes; D008567:Membranes, Artificial; D008903:Minerals; D003094:Collagen; D017886:Durapatite