title: Neuroaminidase reduces interictal spikes in a rat temporal lobe epilepsy model.
abstract: Interictal spikes have been implicated in epileptogenesis and cognitive dysfunction in epilepsy. Unfortunately, antiepileptic drugs have shown poor efficacy in suppressing interictal discharges; novel therapies are needed. Surface charge on neuronal membranes provides a novel target for abolishing interictal spikes. This property can be modulated through the use of neuraminidase, an enzyme that decreases the amount of negatively charged sialic acid. In the present report we determined whether applying neuraminidase to brains of rats with a history of status epilepticus would reduce number of interictal discharges. Following pilocarpine-induced status epilepticus, rats received intrahippocampal injections of neuraminidase, which significantly decreased the number of interictal spikes recorded in the CA1 region. This study provides evidence that sialic acid degradation can reduce the number of interictal spikes. Furthermore, the results suggest that modifying surface charge created by negatively charged sialic acid may provide new opportunities for reducing aberrant epileptiform events in epilepsy.
authors.affiliation: Department of General Physiology of Nervous System, Bogomoletz Institute of Physiology, Kiev, Ukraine.
authors.forename: Dmytro
authors.initials: D
authors.lastname: Isaev
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jonathan K
authors.initials: JK
authors.lastname: Kleen
authors.forename: Pierre Pascal
authors.initials: PP
authors.lastname: Lenck-Santini
authors.forename: Dusit
authors.initials: D
authors.lastname: Adstamongkonkul
authors.forename: Elena
authors.initials: E
authors.lastname: Isaeva
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Holmes
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D003292:Convulsants; D004569:Electroencephalography / Q000187:drug effects*; D004833:Epilepsy, Temporal Lobe / Q000473:pathology / Q000503:physiopathology*; D005071:Evoked Potentials / Q000187:drug effects*; D005911:Gliosis / Q000473:pathology; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D007267:Injections; D007274:Injections, Intraperitoneal; D018021:Lithium Chloride; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology*; D010862:Pilocarpine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012815:Signal Processing, Computer-Assisted; D013226:Status Epilepticus / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000927:Anticonvulsants; D003292:Convulsants; D010862:Pilocarpine; D009439:Neuraminidase; D018021:Lithium Chloride; D019158:N-Acetylneuraminic Acid