title: 2-Deoxy-D-glucose reverses the Indian red scorpion venom-induced cardiopulmonary abnormalities in anesthetized rats.
abstract: Role of 2-Deoxy-D-glucose (2-DG) in reversing the Indian red scorpion (Mesobuthus tamulus concanesis Pocock, MBT) venom-induced toxicity was examined. Femoral arterial pressure, ECG and respiratory movements were recorded in urethane anesthetized rats. Plasma glucose and serum insulin levels were also estimated. Intravenous injection of 5 mg/kg MBT venom produced immediate decrease in mean arterial pressure, heart rate and respiratory frequency followed by an increase and subsequent progressive decrease. ECG pattern exhibited ischaemic changes. There was hyperinsulinemia after venom without corresponding decrease in plasma glucose. The animals died within 37 +/- 9 min and demonstrated significant increase in pulmonary water content. 2-DG pretreatment (0.5 g/kg, iv) improved the cardiopulmonary abnormalities induced by venom and the animals survived for nearly 120 min. There was no hyperinsulinemia and increased pulmonary water content in these animals. In insulin (2 IU/kg) treated rats, the MBT venom-induced cardiopulmonary abnormalities were attenuated and ECG abnormalities were reversed. The pulmonary water content in these animals exhibited a decreasing trend and the animals survived for 120 min. Repaglinide (10 microg/kg, iv) pretreatment failed to reverse the venom-induced cardiopulmonary changes including the increased pulmonary water content. The survival time was similar to venom only group. The present results reveal that 2-DG reverses the venom-induced cardiopulmonary toxicity probably by restoring insulin sensitivity.
authors.affiliation: Department of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Choudhry
authors.forename: R
authors.initials: R
authors.lastname: Pandey
authors.forename: S B
authors.initials: SB
authors.lastname: Deshpande
mesh_terms: D000758:Anesthesia; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002219:Carbamates / Q000494:pharmacology / Q000627:therapeutic use; D018376:Cardiovascular Abnormalities / Q000139:chemically induced / Q000150:complications* / Q000188:drug therapy* / Q000503:physiopathology; D003847:Deoxyglucose / Q000627:therapeutic use*; D006943:Hyperglycemia / Q000150:complications / Q000188:drug therapy; D007194:India; D007328:Insulin / Q000097:blood / Q000494:pharmacology / Q000627:therapeutic use; D008171:Lung Diseases / Q000139:chemically induced / Q000150:complications* / Q000188:drug therapy* / Q000503:physiopathology; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000150:complications / Q000000981:diagnostic imaging / Q000188:drug therapy; D010880:Piperidines / Q000494:pharmacology / Q000627:therapeutic use; D011654:Pulmonary Edema / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy / Q000503:physiopathology; D051381:Rats; D012119:Respiration / Q000187:drug effects; D012604:Scorpion Venoms / Q000037:antagonists & inhibitors / Q000633:toxicity*; D014463:Ultrasonography
chemical_list: D001786:Blood Glucose; D002219:Carbamates; D007328:Insulin; D010880:Piperidines; D012604:Scorpion Venoms; C072379:repaglinide; D003847:Deoxyglucose