title: Syk kinase is phosphorylated in specific areas of the developing nervous system.
abstract: An increasing number of data involve immunoreceptors in brain development, synaptic plasticity and behavior. However it has yet to be determined whether these proteins in fact transmit an immunoreceptor-like signal in non-hematopoietic neuronal cells. The recruitment and activation of the Syk family tyrosine kinases, Syk and ZAP-70, being a critical step in this process, we conducted a thorough analysis of Syk/ZAP-70 expression pattern in nervous tissues. Syk/ZAP-70 is present in neurons of different structures including the cerebellum, the hippocampus, the visual system and the olfactory system. During the olfactory system ontogeny the protein is detected from the 16th embryonic day and persists in adulthood. Importantly, Syk was phosphorylated on tyrosine residues representative of an active form of the kinase in specialized neuronal subpopulations comprising rostral migratory stream neuronal progenitor cells, hippocampal pyramidal cells, retinal ganglion cells and cerebellar granular cells. Phospho-Syk staining was also observed in synapse-rich regions such as the olfactory bulb glomeruli and the retina inner plexiform layer. Furthermore, our work on cultured primary hippoccampal neurons indicates that as for hematopoietic cells, Syk phosphorylation is readily induced upon pervanadate treatment. Therefore, Syk appears to be a serious candidate in connecting immunoreceptors to downstream adaptor/effector molecules in neurons.
authors.affiliation: INSERM U1028, CNRS UMR5292, Lyon Neuroscience Research Center, Neuro-oncology & Neuro-inflammation Team, University of Lyon 1, Lyon F-69000, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Hatterer
authors.forename: Aurélien
authors.initials: A
authors.lastname: Benon
authors.forename: Naura
authors.initials: N
authors.lastname: Chounlamountri
authors.forename: Chantal
authors.initials: C
authors.lastname: Watrin
authors.forename: Julie
authors.initials: J
authors.lastname: Angibaud
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Jouanneau
authors.forename: Hélène
authors.initials: H
authors.lastname: Boudin
authors.forename: Jérôme
authors.initials: J
authors.lastname: Honnorat
authors.forename: Véronique
authors.initials: V
authors.lastname: Pellier-Monnin
authors.forename: Nelly
authors.initials: N
authors.lastname: Noraz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology* / Q000254:growth & development*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D010766:Phosphorylation / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000196:embryology / Q000201:enzymology; D015398:Signal Transduction / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D000072377:Syk Kinase; D051746:ZAP-70 Protein-Tyrosine Kinase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606898:Syk protein, rat; D051746:ZAP-70 Protein-Tyrosine Kinase