title: Synthesis of lipid-oligonucleotide conjugates for RNA interference studies.
abstract: The synthesis of RNA molecules carrying lipids at their 3'-termini and 5'-termini is reported. These conjugates were fully characterized by MALDI-TOF mass spectrometry and HPLC chromatography. The ability of these conjugates to silence gene expression was evaluated in the inhibition of the tumor necrosis factor. All the lipid-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some lipid-siRNA derivatives can exert a slight reduction of gene expression.
authors.affiliation: Institute for Research in Biomedicine, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Edifici Helix, Baldiri Reixac 10, Barcelona, Spain. recgma@cid.csic.es
authors.forename: Santiago
authors.initials: S
authors.lastname: Grijalvo
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Ocampo
authors.forename: José C
authors.initials: JC
authors.lastname: Perales
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D058990:Molecular Targeted Therapy / Q000379:methods; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008055:Lipids; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C484027:oligofectamine