title: Development of a system to sensitively and specifically visualize c-fos mRNA in living cells using bispyrene-modified RNA probes.
abstract: In situ visualization of c-fos mRNA was shown both in fixed cells and in living cells using bispyrene-modified 2'-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fos mRNA was successfully developed.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Waki
authors.forename: Asako
authors.initials: A
authors.lastname: Yamayoshi
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D009843:Oligoribonucleotides / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics* / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry*; D015347:RNA Probes / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D059008:Time-Lapse Imaging / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D009843:Oligoribonucleotides; D016760:Proto-Oncogene Proteins c-fos; D011721:Pyrenes; D015347:RNA Probes; D012333:RNA, Messenger