title: [Analysis of gene expression patterns in an irinotecan-resistance colon cancer cell by cDNA microarray].
abstract: OBJECTIVE
  To study the mechanism of irinotecan-resistant colon cancer by analyzing the differential gene expression patterns with cDNA microarray.
  
  
  METHODS
  Total RNA was purified from irinotecan-sensitive colonic cancer cell line, SW480 and its irinotecan-resistance cell line, SW480/CPT. The cRNA retro-transcribed from RNA were labeled with Cy3 fluorescence as probes. The probes were hybridized with Agilent gene chips and the fluorescence images of the chips were obtained with Axon 4000B scanner as well as analyzed with Genepix 3.0 software. The microarray results were confirmed by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Of the 1598 genes with altered expressions, there were 911 up-regulated genes and 687 down-regulated genes. Glutathione S-Transferase (GST) isoenzyme family GSTA such as GSTA1, GSTA2, GSTA3 and GSTA5 were significantly up-regulated. The expression levels of many Zinc finger protein family members (ZNF) were also differentially regulated.
  
  
  CONCLUSIONS
  GSTA and ZNF subunit genes might play an important regulation role in the irinotecan resistance of colon cancer.
authors.affiliation: Department of Abdominal Cancer, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Xiang-zheng
authors.initials: XZ
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: Lang
authors.forename: Qiu-lin
authors.initials: QL
authors.lastname: Tang
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D000077146:Irinotecan; D020411:Oligonucleotide Array Sequence Analysis; D059467:Transcriptome; D016335:Zinc Fingers
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000077146:Irinotecan; D005982:Glutathione Transferase; D002166:Camptothecin