title: Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis.
abstract: Thirteen Gram-positive-staining coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n=12) and a teat apex (n=1). The results of sequence analysis of the 16S rRNA gene and two housekeeping genes, rpoB and tuf, and DNA fingerprinting with amplified fragment length polymorphism (AFLP) analysis showed that the isolates formed a separate branch within the genus Staphylococcus. The phylogenetically most closely related species were Staphylococcus hyicus and Staphylococcus chromogenes. DNA-DNA hybridization with S. hyicus DSM 20459(T) and S. chromogenes DSM 20674(T) confirmed that the isolates belonged to a separate species. The predominant fatty acids were i-C(15:0), ai-C(15:0), i-C(17:0) and C(20:0) and the peptidoglycan type was A3α L-Lys-Gly(5). Based on the results of genotypic and phenotypic analyses, it is proposed that the thirteen isolates represent a novel species, for which the name Staphylococcus agnetis sp. nov. is proposed. Strain 6-4(T) (=DSM 23656(T)=CCUG 59809(T)) is the type strain.
authors.affiliation: Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, PO Box 57, 00014 Helsinki, Finland.
authors.forename: Suvi
authors.initials: S
authors.lastname: Taponen
authors.affiliation: Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Karlien
authors.initials: K
authors.lastname: Supré
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
authors.forename: Veerle
authors.initials: V
authors.lastname: Piessens
authors.affiliation: Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: Van Coillie
authors.affiliation: Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Sarne
authors.initials: S
authors.lastname: De Vliegher
authors.affiliation: Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, PO Box 66, 00014 Helsinki, Finland.
authors.forename: Joanna M K
authors.initials: JMK
authors.lastname: Koort
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D058070:Asymptomatic Diseases; D015373:Bacterial Typing Techniques; D002417:Cattle; D016000:Cluster Analysis; D003030:Coagulase / Q000378:metabolism*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010444:Peptide Elongation Factor Tu / Q000235:genetics; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003030:Coagulase; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D010444:Peptide Elongation Factor Tu