title: [Kinetic spectrophotometric study on hemolytic process of polysorbate 80].
abstract: To establish kinetic assay method for the analysis of hemolysis and to investigate dynamic hemolytic process of polysorbate 80. The UV-VIS spectrum of heme changes when hemoglobin is released continuously during the hemolytic process. Therefore, dynamic hemolytic curve was determined as a new way to characterize the kinetic process of interaction between polysorbate 80 and red blood cells. The effect of polysorbate 80 on blood cells could be perfectly investigated by the hemolytic dynamics. Dynamic hemolytic parameters of polysorbate 80 were calculated according to the hemolytic curves. The constants of hemolytic rate and maximum hemolytic rate of polysorbate 80 had fine linear relationships at the range of 1-20 mg x mL(-1) and 5-20 mg x mL(-1), respectively. In comparison with the present official method such as macroscopic observation and static spectrophotometric methods, kinetic spectrophotometry has the advantages of real time, on-line determination, sensitive, objective, good reproducibility and 2-dimensional information acquired. Therefore, as a biological technique, kinetic spectrophotometry could be applied to evaluate the quality of polysorbate 80 and to screen other solubilizing excipients.
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Shao-Feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Tang
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Yue-sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000818:Animals; D004355:Drug Stability; D004912:Erythrocytes / Q000737:chemistry; D005079:Excipients / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis; D007700:Kinetics; D011136:Polysorbates / Q000737:chemistry*; D011817:Rabbits; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D005079:Excipients; D006454:Hemoglobins; D011136:Polysorbates