title: New triterpenoids from the tubers of Corydalis ternata: structural elucidation and bioactivity evaluation.
abstract: Two new triterpene glycosides, coryternic acid 3-O-β-D-glucuronopyranoside (1) and coryternic acid 3-O-β-D-glucuronopyranoside-6'-O-methyl ester (2), were isolated from a MeOH extract of the tubers of Corydalis ternata. Acidic hydrolysis of 1 and 2 yielded a new triterpene as their aglycone, coryternic acid (3). The structures of these new compounds were determined through spectral analysis, including extensive 2D-NMR data. In this study we reported that triterpenoids were first isolated from the genus Corydalis. Compound 2 exhibited significant cytotoxicity against the A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines (IC50 = 15.16, 17.07, 13.32, and 11.95 µM, respectively) and significantly reduced NO production in lipopolysaccharide (LPS)-activated microglia/BV-2 cells with an IC50 value of 16.2 µM without cell toxicity.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Il Kyun
authors.initials: IK
authors.lastname: Lee
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Jei Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Eunjung
authors.initials: E
authors.lastname: Moon
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival; D031311:Corydalis / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D055670:Medicine, Korean Traditional; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014315:Triterpenes; D009569:Nitric Oxide