title: Effects of a moderate-intensity static magnetic field and adriamycin on K562 cells.
abstract: The aim of this study was to investigate whether a moderate-intensity static magnetic field (SMF) can enhance the killing effect of adriamycin (ADM) on K562 cells, and to explore the effects of SMF combined with ADM on K562 cells. We analyzed the metabolic activity of cells, cell cycle distribution, DNA damage, change in cell ultrastructure, and P-glycoprotein (P-gp) expression after K562 cells were exposed continuously to a uniform 8.8 mT SMF for 12 h, with or without ADM. Our results showed that the SMF combined with ADM (25 ng/ml) significantly inhibited the metabolic activity of K562 cells (P < 0.05), while neither ADM nor the SMF alone affected the metabolic activity of these cells. Cell ultrastructure was altered in the SMF + ADM group. For example, cell membrane was depressed, some protuberances were observable, and vacuoles in the cytoplasm became larger. Cells were arrested at the G2/M phase and DNA damage increased after cells were treated with the SMF plus ADM. ADM also induced the P-gp expression. In contrast, in the SMF group and SMF + ADM group, the P-gp expression was decreased compared with the ADM group. Taken together, our results showed that the 8.8 mT SMF enhanced the cytotoxicity potency of ADM on K562 cells, and the decrease in P-gp expression may be one reason underlying this effect.
authors.affiliation: College of Life Science, Shaanxi Normal University, Xi'an, Shaanxi, P. R. China. qhsnnu@126.com
authors.forename: Qi
authors.initials: Q
authors.lastname: Hao
authors.forename: Chen
authors.initials: C
authors.lastname: Wenfang
authors.forename: Ai
authors.initials: A
authors.lastname: Xia
authors.forename: Wei
authors.initials: W
authors.lastname: Qiang
authors.forename: Liu
authors.initials: L
authors.lastname: Ying
authors.forename: Zhang
authors.initials: Z
authors.lastname: Kun
authors.forename: Sun
authors.initials: S
authors.lastname: Runguang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004249:DNA Damage; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects; D006801:Humans; D020014:K562 Cells; D008280:Magnetics; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D004317:Doxorubicin