title: A recombinant α-dioxygenase from rice to produce fatty aldehydes using E. coli.
abstract: Fatty aldehydes are an important group of fragrance and flavor compounds that are found in different fruits and flowers. A biotechnological synthesis of fatty aldehydes based on Escherichia coli cells expressing an α-dioxygenase (αDOX) from Oryza sativa (rice) is presented. α-Dioxygenases are the initial enzymes of α-oxidation in plants and oxidize long and medium-chain C(n) fatty acids to 2-hydroperoxy fatty acids. The latter are converted to C(n-1) fatty aldehydes by spontaneous decarboxylation. Successful expression of αDOX in E. coli was proven by an in vitro luciferase assay. Using resting cells of this recombinant E. coli strain, conversion of different fatty acids to the respective fatty aldehydes shortened by one carbon atom was demonstrated. The usage of Triton X 100 improves the conversion rate up to 1 g aldehyde per liter per hour. Easy reuse of the cells was demonstrated by performing a second biotransformation without any loss of biocatalytic activity.
authors.affiliation: DECHEMA e.V. Karl-Winnacker-Institut, Biochemical Engineering, Theodor-Heuss-Allee 25, 60486 Frankfurt/Main, Germany.
authors.forename: Fenja
authors.initials: F
authors.lastname: Kaehne
authors.forename: Markus
authors.initials: M
authors.lastname: Buchhaupt
authors.forename: Jens
authors.initials: J
authors.lastname: Schrader
mesh_terms: D000447:Aldehydes; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis* / Q000378:metabolism; D015870:Gene Expression; D012275:Oryza / Q000201:enzymology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000447:Aldehydes; D005227:Fatty Acids; D010940:Plant Proteins; D011994:Recombinant Proteins; C001634:fatty aldehyde; D049308:Dioxygenases