title: In vitro antimicrobial properties of caprylic acid, monocaprylin, and sodium caprylate against Dermatophilus congolensis.
abstract: OBJECTIVE
  To determine antimicrobial effects of caprylic acid and its derivatives, monocaprylin and sodium caprylate, on Dermatophilus congolensis and to determine effects of caprylic acid on the ultrastructure of D congolensis by use of transmission electron microscopy (TEM).
  
  
  METHODS
  3 strains of D congolensis (33411, 33413, and 14639).
  
  
  METHODS
  Strains of D congolensis were incubated separately under anaerobic conditions at 37°C for up to 48 hours in brain heart infusion (BHI) broth that was supplemented with various concentrations of caprylic acid (7.5, 12.5, 15, 17.5, or 20mM), monocaprylin (2.5, 5, 7.5, or 10mM), or sodium caprylate (15, 50, 60, 70, 100, or 120mM) or contained no antimicrobial treatment. After incubation, bacterial counts were determined by means of plating in triplicate on BHI-agar plates. Caprylic acid-treated or untreated D congolensis samples were embedded in epoxide resin for TEM; cross sections were examined for structural damage.
  
  
  RESULTS
  Minimum inhibitory concentrations of caprylic acid, monocaprylin, and sodium caprylate against D congolensis were 7.5, 2.5, and 15 mM, respectively. Minimum bactericidal concentrations of caprylic acid, monocaprylin, and sodium caprylate against D congolensis were 15, 5, and 70 mM, respectively. Examination via TEM revealed that a 15-mM concentration of caprylic acid disintegrated the plasma membrane of D congolensis.
  
  
  CONCLUSIONS
  Results indicated that caprylic acid, monocaprylin, and sodium caprylate could potentially be used to treat D congolensis infections. However, in vivo studies should be undertaken to determine whether these compounds can be considered as treatment options.
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Satyender Rao
authors.initials: SR
authors.lastname: Valipe
authors.forename: Jenifer Ann
authors.initials: JA
authors.lastname: Nadeau
authors.forename: Thirunavukkarasu
authors.initials: T
authors.lastname: Annamali
authors.forename: Kumar
authors.initials: K
authors.lastname: Venkitanarayanan
authors.forename: Thomas
authors.initials: T
authors.lastname: Hoagland
mesh_terms: D000192:Actinomycetales / Q000187:drug effects* / Q000648:ultrastructure; D000193:Actinomycetales Infections / Q000188:drug therapy / Q000662:veterinary*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002210:Caprylates / Q000494:pharmacology*; D005989:Glycerides / Q000494:pharmacology; D006734:Horse Diseases / Q000188:drug therapy / Q000382:microbiology*; D006736:Horses; D017192:Skin Diseases, Bacterial / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000900:Anti-Bacterial Agents; D002210:Caprylates; D005989:Glycerides; C031492:octanoic acid; C025343:monooctanoin