title: Presence of B-cell clones in T-cell lymphoma.
abstract: OBJECTIVE
  The presence of B-cell clones in 76 cases with peripheral T-cell lymphoma (PTCL) and precursor T-lymphoblastic lymphoma (T-LBL) and its correlation with Epstein-Barr virus (EBV) was studied.
  
  
  METHODS
  DNA was extracted from paraffin sections and/or fresh-frozen samples and then used for clonality analysis using a modified BIOMED-2 polymerase chain reaction (PCR)-based method.
  
  
  RESULTS
  T- and B-cell clones were detected in 59 (77.6%) and 14 (18.4%) of 76 patients, respectively: 90% and 30% of cases with PTCL, not otherwise specified, 76.4% and 17.6% of cases with angioimmunoblastic T-cell lymphoma, 77% and 7.6% of cases with adult T-cell lymphoma, 50% and 0% of cases with anaplastic large T-cell lymphoma, 62.5% and 12.5% of cases with T-LBL, and 50% and 0% of cases with intestinal T-cell lymphoma, respectively. Histological and immunohistochemical analyses revealed the presence of large B cells in lesional tissues, which were occasionally monoclonal. The presence of B-cell clones was highly associated with EBV positivity, as revealed by in situ hybridization. In two cases that were evaluated by serial histological and molecular examination, EBV-positive cells persisted in one and disappeared in the other.
  
  
  CONCLUSIONS
  These findings suggest a role for EBV in the evolution of B-cell clones in T-cell lymphomas.
authors.affiliation: Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
authors.forename: Mona A A
authors.initials: MA
authors.lastname: Zaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Wada
authors.forename: Masaharu
authors.initials: M
authors.lastname: Kohara
authors.forename: Junichiro
authors.initials: J
authors.lastname: Ikeda
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Hori
authors.forename: Shigeki
authors.initials: S
authors.lastname: Fujita
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ogawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hino
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Kanakura
authors.forename: Eiichi
authors.initials: E
authors.lastname: Morii
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Aozasa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology* / Q000821:virology; D002999:Clone Cells / Q000276:immunology / Q000473:pathology / Q000821:virology; D005260:Female; D015322:Gene Rearrangement, B-Lymphocyte; D015329:Gene Rearrangement, T-Lymphocyte; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017403:In Situ Hybridization; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D055815:Young Adult