title: Preparative isolation and purification of cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale was successfully used in isolation and purification of cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by using a two-phase solvent system composed of n-hexane-methanol-water (5:4:1, v/v/v). The targeted compounds were isolated, collected, purified by HSCCC in the head-tail mode, and then analyzed by gas chromatography (GC). A total of 12.72 ± 0.22 mg of cuminaldehyde and 10.61 ± 0.27 mg of p-menta-1,4-dien-7-al were obtained from 50 mg of the essential oil of C. cyminum L. in less than 6 h, with purities of 95.42% and 97.21%, respectively. In addition to GC-EI/MS, the identity of the cuminaldehyde was further confirmed with the retention time using the method of standard addition, while, the structural identification of p-menta-1,4-dien-7-al was performed with GC-EI/MS, (1)H NMR and (1)H-(1)H COSY.
authors.affiliation: College of Food Science & Nutritional Engineering, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, 17 Tsinghua East Road, Beijing 100083, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Chen
authors.forename: Xuefang
authors.initials: X
authors.lastname: Hu
authors.forename: Jingming
authors.initials: J
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Ni
mesh_terms: D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000379:methods; D028527:Cuminum / Q000737:chemistry*; D000081007:Cymenes; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification*; D010938:Plant Oils / Q000737:chemistry; D012639:Seeds / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D000081007:Cymenes; D039821:Monoterpenes; D010938:Plant Oils; C007165:cuminaldehyde