title: Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm.
abstract: We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs.
authors.affiliation: Department of Medical Biochemistry & Microbiology, Uppsala University, BMC, 75123 Uppsala, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Tranell
authors.forename: Susanne
authors.initials: S
authors.lastname: Tingsborg
authors.forename: Eva Maria
authors.initials: EM
authors.lastname: Fenyö
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwartz
mesh_terms: D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D005091:Exons; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics*; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D010957:Plasmids; D011533:Proviruses / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000068103:Serine-Arginine Splicing Factors; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000378:metabolism; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism; D054321:rev Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism; D000086082:Viroporin Proteins
chemical_list: D054298:Human Immunodeficiency Virus Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D000068103:Serine-Arginine Splicing Factors; D054334:Viral Regulatory and Accessory Proteins; D054299:env Gene Products, Human Immunodeficiency Virus; D054321:rev Gene Products, Human Immunodeficiency Virus; C489470:SRSF6 protein, human; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins