title: [The protective effects of sodium selenite and aloin against ultraviolet A radiation].
abstract: OBJECTIVE
  To investigate the protective effects of sodium selenite and aloin against ultraviolet A (UVA) radiation on the dermal fibroblasts including the suppression of proliferation, oxidative damage and collagen synthesis.
  
  
  METHODS
  Human dermal fibroblasts were divided into the negative control group, the positive control UVA radiation group, the sodium selenite treatment plus UVA radiation group and the aloin treatment plus UVA radiation group, which were incubated with sodium selenite or aloin and irradiated with 5 J/cm2 UVA respectively. The MTT spectrophotometry and biochemical assay were used to determine the proliferation, collagen level and the SOD and GSH-Px activity of the fibroblast cells after radiation.
  
  
  RESULTS
  5 J/cm2 UVA affect the proliferation, collage protein, SOD and GSH-Px activity in the fibroblast cells (P < 0.05). Sodium selenite (10-100 microg/L) and aloin (1-100 mg/L) could enhance the proliferation and the SOD and GSH-Px activity. A increased collagen synthesis in dose dependant manner was also observed (P < 0.05).
  
  
  CONCLUSIONS
  Sodium selenite and aloin in a certain range of concentration have protective effects on ultraviolet radiation induced fibroblast proliferation inhibition, oxidative injury, and decreased collagen synthesis.
authors.affiliation: Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Ran
authors.initials: R
authors.lastname: Ji
authors.forename: Xi
authors.initials: X
authors.lastname: Lü
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Wan
authors.forename: Xian
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D002478:Cells, Cultured; D020405:Dermis / Q000473:pathology; D004642:Emodin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005347:Fibroblasts / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D008297:Male; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects; D018038:Sodium Selenite / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D011837:Radiation-Protective Agents; D018038:Sodium Selenite; D004642:Emodin; C006457:alloin