title: Trace analysis of D-tyrosine in biological samples by microchip electrophoresis with laser induced fluorescence detection.
abstract: A rapid and sensitive microchip electrophoresis (MCE) method with laser induced fluorescence (LIF) detection has been developed for the quantification of D-tyrosine (Tyr) in biological samples. The assay was performed using a MCE-LIF system with glass/poly(dimethylsiloxane) (PDMS) hybrid microchip after pre-column derivatization of amino acids with fluorescein isothiocyanate (FITC). Chiral separation of the derivatives was achieved by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) using γ-CD as chiral selector in the running buffer. D/L-Tyr enantiomer was well separated in less than 140s. The limit of detection (S/N=3) was 3.3 × 10(-8) M. Using the present method, D-Tyr level in human plasma was found to vary significantly from normal humans to patients suffering from renal failure.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D016014:Linear Models; D051437:Renal Insufficiency; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014443:Tyrosine / Q000032:analysis* / Q000097:blood / Q000737:chemistry
chemical_list: D014443:Tyrosine; D016650:Fluorescein-5-isothiocyanate