title: Structure, expression differentiation and evolution of duplicated fiber developmental genes in Gossypium barbadense and G. hirsutum.
abstract: BACKGROUND
  Both Gossypium hirsutum and G. barbadense probably originated from a common ancestor, but they have very different agronomic and fiber quality characters. Here we selected 17 fiber development-related genes to study their structures, tree topologies, chromosomal location and expression patterns to better understand the interspecific divergence of fiber development genes in the two cultivated tetraploid species.
  
  
  RESULTS
  The sequence and structure of 70.59% genes were conserved with the same exon length and numbers in different species, while 29.41% genes showed diversity. There were 15 genes showing independent evolution between the A- and D-subgenomes after polyploid formation, while two evolved via different degrees of colonization. Chromosomal location showed that 22 duplicate genes were located in which at least one fiber quality QTL was detected. The molecular evolutionary rates suggested that the D-subgenome of the allotetraploid underwent rapid evolutionary differentiation, and selection had acted at the tetraploid level. Expression profiles at fiber initiation and early elongation showed that the transcripts levels of most genes were higher in Hai7124 than in TM-1. During the primary-secondary transition period, expression of most genes peaked earlier in TM-1 than in Hai7124. Homeolog expression profile showed that A-subgenome, or the combination of A- and D-subgenomes, played critical roles in fiber quality divergence of G. hirsutum and G. barbadense. However, the expression of D-subgenome alone also played an important role.
  
  
  CONCLUSIONS
  Integrating analysis of the structure and expression to fiber development genes, suggests selective breeding for certain desirable fiber qualities played an important role in divergence of G. hirsutum and G. barbadense.
authors.affiliation: National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Zhu
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Han
authors.forename: Junhong
authors.initials: J
authors.lastname: Lv
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Xu
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Zhang
authors.forename: Wangzhen
authors.initials: W
authors.lastname: Guo
mesh_terms: D001947:Breeding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence; D032561:Cotton Fiber; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020131:Genes, Duplicate; D017343:Genes, Plant; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D010802:Phylogeny; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D057891:Tetraploidy