title: In vitro anti-HIV-1 activity of the aqueous extract of Asterina pectinifera.
abstract: An aqueous extract of starfish, Asterina pectinifera, was investigated for its anti-HIV-1 efficiency in vitro on human T-cell lines. A. pectinifera significantly maintained the viability of HIV-infected cells as much as 86% of the untreated infected control cells at the non-toxic concentrations (0.05~4 mg/mL) in CEM-SS cells. Anti-HIV-1 activity of A. pectinifera extract was further supported by quantification of syncytia formation, reverse transcriptase activity and Western blot analysis in C8166, CEM-SS and H9 cells, respectively. Current results demonstrated a notable inhibition of HIV-1 induced syncytia formation and HIV-1 reverse transcriptase activity assay with EC50 of 0.71 mg/mL and 0.65 mg/mL, respectively. Moreover, A. pectinifera extract treatment decreased the production of p24 protein and gene expression of HIV-1 viral infection factor in a dose-dependent manner according to immunoblot and reverse transcription polymerase chain reaction analysis. In the light of current study, it can be concluded that A. pectinifera contains highly potential anti-HIV-1 components and a further investigation for active compound isolation is urged.
authors.affiliation: Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Fatih
authors.initials: F
authors.lastname: Karadeniz
authors.forename: Mustafa Zafer
authors.initials: MZ
authors.lastname: Karagozlu
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology*; D047288:Asterina / Q000737:chemistry*; D002457:Cell Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D015726:Giant Cells / Q000187:drug effects; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D013601:T-Lymphocytes / Q000821:virology
chemical_list: D019380:Anti-HIV Agents; D002457:Cell Extracts; D016655:HIV Core Protein p24