title: Increase of BM-MSC proliferation using L-DOPA on titanium surface in vitro.
abstract: In order to increase biocompatibility, many dental implants have been studied by immobilization of biomolecules on biomaterials. We used l-3,4-dihydroxyphenylalanine (L-DOPA) as a biomolecule for surface-modified titanium. Water contact angles of nontreated titanium discs (negative control), etched titanium discs (positive control), and titanium discs treated with L-DOPA following the etching process (experimental group) were 82.4 ± 5.7°, 67.1 ± 0.56°, and 44.15 ± 0.91°, respectively. Using atomic force microscopy images, we were able to find L-DOPA, which adhered to the titanium surface. The number of human bone marrow mesenchymal stem cells (BM-MSCs) in the experimental group was much higher than that of cells in any other group. Quantification values of amine groups in the positive control and experimental groups were approximately 3 and 7.5 µg, respectively. Therefore, findings from our research suggested the possibility of a causal link between increased L-DOPA content and cell proliferation in BM-MSCs. Moreover, coating of the discs with L-DOPA can result in greater hydrophilicity of the titanium surface and enhancement of cell adhesion and mitochondrial activity.
authors.affiliation: Department of Medical Biotechnology, Dongguk University, 3-26 Pil-dong, Chung-gu, Seoul 100-715, Korea.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Jung-Keug
authors.initials: JK
authors.lastname: Park
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Hyun-Suk
authors.initials: HS
authors.lastname: Jung
authors.forename: Kug-Sun
authors.initials: KS
authors.lastname: Hong
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Kwang-Bum
authors.initials: KB
authors.lastname: Park
authors.forename: Seok-Kyu
authors.initials: SK
authors.lastname: Choi
authors.forename: Young-Kwon
authors.initials: YK
authors.lastname: Seo
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D015259:Dopamine Agents / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007980:Levodopa / Q000737:chemistry / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D015259:Dopamine Agents; D007980:Levodopa; D014025:Titanium