title: Stress induced β subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase.
abstract: We here report in Pisum sativum system a novel protein-protein interaction of β-subunit of heterotrimeric G-proteins (PsGβ) with a Mitogen activated protein kinase (PsMPK3) during cDNA library screening by yeast-two-hybrid assay. The transcript of these two genes also showed co-regulation under abscisic acid (ABA) and methyl jasmonate (MeJA) treatments. The protein-protein interaction was further validated by performing one-to-one interaction and β-galactosidase assay in yeast system. β-subunit of G-proteins from a heterologous system Oryzae sativa also showed interaction with PsMPK3. The interaction between PsGβ and PsMPK3 was further confirmed by in vitro protein-protein interaction. This suggested that MPK3 function as effector molecule for Gβ, which may helps in the regulation of stomatal functioning. These findings also provide an evidence for a possible cross-talk between MPK3 and G-protein-mediated signaling pathways in plants.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Bhardwaj
authors.forename: Arsheed Hussain
authors.initials: AH
authors.lastname: Sheikh
authors.forename: Alok Krishna
authors.initials: AK
authors.lastname: Sinha
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000378:metabolism; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology; D054883:Oxylipins / Q000378:metabolism; D018532:Pisum sativum / Q000201:enzymology*; D010940:Plant Proteins / Q000378:metabolism*; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D000040:Abscisic Acid; C072239:methyl jasmonate; D020928:Mitogen-Activated Protein Kinases; D020962:Heterotrimeric GTP-Binding Proteins