title: Characterization of the Mycobacterium avium subsp. paratuberculosis laminin-binding/histone-like protein (Lbp/Hlp) which reacts with sera from patients with Crohn's disease.
abstract: Mycobacterium avium subsp. paratuberculosis (Map) causes a chronic enteric disease in ruminants, called paratuberculosis or Johne's disease. The current model proposes that after ingestion by the host, Map crosses the intestinal barrier via internalization by the M cells. Experimental observations suggest, however, that Map may also transcytose the intestinal wall via the enterocytes, but the mechanisms involved in this process remain poorly understood. Cytoadherence assays performed on epithelial cells with Map revealed that the addition of laminin to the cell culture increases adhesion. A Map protein was isolated by heparin-Sepharose chromatography and identified as a laminin-binding protein like. The gene encoding this protein named Lbp/Hlp was identified in the Map genome sequence at locus MAP3024 (annotated Hup B). The deduced Map Lbp/Hlp amino acid sequence reveals 80% identity with that reported for other mycobacteria. The C-terminal domain involved in adhesion is mainly composed of arginine and lysine residues modified by methylation. In vitro tests demonstrated that recombinant Lbp/Hlp binds laminin, heparin, collagen and epithelial cells. Interestingly, we found that this adhesin corresponds to the antigen described as the target of pANCA and serum antibodies of patients with Crohn's disease.
authors.affiliation: INRA, UR1282, Infectiologie Animale, Santé Publique (IASP-311), F-37380 Nouzilly, France.
authors.forename: Louise H
authors.initials: LH
authors.lastname: Lefrançois
authors.forename: Céline
authors.initials: C
authors.lastname: Pujol
authors.forename: Christelle C
authors.initials: CC
authors.lastname: Bodier
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Teixeira-Gomez
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Rosso
authors.forename: Dominique
authors.initials: D
authors.lastname: Raze
authors.forename: André Alves
authors.initials: AA
authors.lastname: Dias
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Hugot
authors.forename: Ofelia
authors.initials: O
authors.lastname: Chacon
authors.forename: Raul G
authors.initials: RG
authors.lastname: Barletta
authors.forename: Camille
authors.initials: C
authors.lastname: Locht
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Vidal Pessolani
authors.forename: Franck
authors.initials: F
authors.lastname: Biet
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism; D003424:Crohn Disease / Q000276:immunology*; D005260:Female; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007797:Laminin; D006493:Heparin; D003094:Collagen