title: Comparison of radiograph-based texture analysis and bone mineral density with three-dimensional microarchitecture of trabecular bone.
abstract: OBJECTIVE
  Hip fracture is a serious health problem and textural methods are being developed to assess bone quality. The authors aimed to perform textural analysis at femur on high-resolution digital radiographs compared to three-dimensional (3D) microarchitecture comparatively to bone mineral density.
  
  
  METHODS
  Sixteen cadaveric femurs were imaged with an x-ray device using a C-MOS sensor. One 17 mm square region of interest (ROI) was selected in the femoral head (FH) and one in the great trochanter (GT). Two-dimensional (2D) textural features from the co-occurrence matrices were extracted. Site-matched measurements of bone mineral density were performed. Inside each ROI, a 16 mm diameter core was extracted. Apparent density (Dapp) and bone volume proportion (BV/TV(Arch)) were measured from a defatted bone core using Archimedes' principle. Microcomputed tomography images of the entire length of the core were obtained (Skyscan 1072) at 19.8 microm of resolution and usual 3D morphometric parameters were computed on the binary volume after calibration from BV/TV(Arch). Then, bone surface/bone volume, trabecular thickness, trabecular separation, and trabecular number were obtained by direct methods without model assumption and the structure model index was calculated.
  
  
  RESULTS
  In univariate analysis, the correlation coefficients between 2D textural features and 3D morphological parameters reached 0.83 at the FH and 0.79 at the GT. In multivariate canonical correlation analysis, coefficients of the first component reached 0.95 at the FH and 0.88 at the GT.
  
  
  CONCLUSIONS
  Digital radiographs, widely available and economically viable, are an alternative method for evaluating bone microarchitectural structure.
authors.affiliation: Caractéristation du Tissu Osseux par Imagerie, U658 Inserm, Orleans, France.
authors.forename: P
authors.initials: P
authors.lastname: Ranjanomennahary
authors.forename: S Sevestre
authors.initials: SS
authors.lastname: Ghalila
authors.forename: D
authors.initials: D
authors.lastname: Malouche
authors.forename: A
authors.initials: A
authors.lastname: Marchadier
authors.forename: M
authors.initials: M
authors.lastname: Rachidi
authors.forename: Cl
authors.initials: C
authors.lastname: Benhamou
authors.forename: C
authors.initials: C
authors.lastname: Chappard
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015519:Bone Density; D005260:Female; D005269:Femur / Q000033:anatomy & histology / Q000000981:diagnostic imaging* / Q000502:physiology*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011856:Radiographic Image Enhancement / Q000379:methods*