title: Migration and homeostasis of naive T cells depends on coronin 1-mediated prosurvival signals and not on coronin 1-dependent filamentous actin modulation.
abstract: Coronins are WD repeat-containing proteins highly conserved in the eukaryotic kingdom implicated in the regulation of F-actin. Mammalian coronin 1, one of the most conserved isoforms expressed in leukocytes, regulates survival of T cells, which has been suggested to be due to its role in preventing F-actin-induced apoptosis. In this study, we come to a different conclusion. We show that coronin 1 does not modulate F-actin and that induction of F-actin failed to induce apoptosis. Instead, coronin 1 was required for providing prosurvival signals, in the absence of which T cells rapidly underwent apoptosis. These results argue against a role for coronin 1 in F-actin-mediated T cell apoptosis and establish coronin 1 as an essential regulator of the balance between prosurvival and proapoptotic signals in naive T cells.
authors.affiliation: Biozentrum, University of Basel, CH 4056 Basel, Switzerland.
authors.forename: Philipp
authors.initials: P
authors.lastname: Mueller
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Liu
authors.forename: Jean
authors.initials: J
authors.lastname: Pieters
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism* / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D019703:Calcineurin / Q000378:metabolism / Q000502:physiology; D020013:Calcium Signaling / Q000235:genetics / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D002470:Cell Survival / Q000235:genetics / Q000276:immunology*; D006706:Homeostasis / Q000235:genetics / Q000276:immunology*; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000235:genetics / Q000276:immunology
chemical_list: D000199:Actins; D051017:Apoptosis Regulatory Proteins; D008024:Ligands; D008840:Microfilament Proteins; D011948:Receptors, Antigen, T-Cell; C072061:coronin proteins; D019703:Calcineurin