title: Juvenile hormone binding protein core promoter is TATA-driven with a suppressory element.
abstract: The hemolymph juvenile hormone binding protein (JHBP) plays a key role in transporting juvenile hormone (JH) to target tissues and in protecting JH from the degradation by nonspecific esterases. Juvenile hormone esterase (JHE) removes JH signal at precisely defined insect developmental stages. The functional analysis of regulatory elements in the core promoter has been described only for the jhe gene. In this report we define the core promoter functional elements in the Galleria mellonella jhbp gene. It appears that jhbp core promoter is under strong control of TATA box and the transcription site (tss). In contrast to regulation of the jhe gene, the jhbp core promoter contains a sequence which directly suppresses jhbp expression. Evidences are provided for the contribution of the will die slowly (WDS) suppressory protein in jhbp basal transcription. We have also shown that the activity of the jhbp core promoter can be regulated by JHIII, and lesser so by 20E.
authors.affiliation: Department of Biochemistry, Faculty of Chemistry, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Niewiadomska-Cimicka
authors.forename: Marcin
authors.initials: M
authors.lastname: Schmidt
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ożyhar
authors.forename: Davy
authors.initials: D
authors.lastname: Jones
authors.forename: Grace
authors.initials: G
authors.lastname: Jones
authors.forename: Marian
authors.initials: M
authors.lastname: Kochman
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004441:Ecdysterone / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D018411:Spodoptera; D016385:TATA Box / Q000235:genetics*; D024363:Transcription Initiation Site; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D005229:Fatty Acids, Monounsaturated; D019476:Insect Proteins; C035606:juvenile hormone-binding protein, insect; C058633:methyl 6,7-10,11-bis(epoxy)-3,7,11-trimethyl-2-dodecenoate; D004441:Ecdysterone; D008156:Luciferases