title: Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.
abstract: Eubacteria and archaea contain a variety of actin-like proteins (ALPs) that form filaments with surprisingly diverse architectures, assembly dynamics, and cellular functions. Although there is much data supporting differences between ALP families, there is little data regarding conservation of structure and function within these families. We asked whether the filament architecture and biochemical properties of the best-understood prokaryotic actin, ParM from plasmid R1, are conserved in a divergent member of the ParM family from plasmid pB171. Previous work demonstrated that R1 ParM assembles into filaments that are structurally distinct from actin and the other characterized ALPs. They also display three biophysical properties thought to be essential for DNA segregation: 1) rapid spontaneous nucleation, 2) symmetrical elongation, and 3) dynamic instability. We used microscopic and biophysical techniques to compare and contrast the architecture and assembly of these related proteins. Despite being only 41% identical, R1 and pB171 ParMs polymerize into nearly identical filaments with similar assembly dynamics. Conservation of the core assembly properties argues for their importance in ParM-mediated DNA segregation and suggests that divergent DNA-segregating ALPs with different assembly properties operate via different mechanisms.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Rivera
authors.forename: Justin M
authors.initials: JM
authors.lastname: Kollman
authors.forename: Jessica K
authors.initials: JK
authors.lastname: Polka
authors.forename: David A
authors.initials: DA
authors.lastname: Agard
authors.forename: R Dyche
authors.initials: RD
authors.lastname: Mullins
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry; D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000737:chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007700:Kinetics; D008954:Models, Biological; D010710:Phosphates / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry; D012542:Scattering, Radiation
chemical_list: D000199:Actins; D029968:Escherichia coli Proteins; C471255:ParM protein, E coli; D010710:Phosphates; D011108:Polymers; D011506:Proteins; D000255:Adenosine Triphosphate; D004247:DNA