title: Zygosaccharomyces gambellarensis sp. nov., an ascosporogenous yeast isolated from an Italian 'passito' style wine.
abstract: Yeast strains were isolated from Vin Santo of Gambellara, a sweet white wine with the specificity of Controlled Designation of Origin produced from off-vine overripened grapes in the Veneto region (Italy). Comparative sequence analysis of the 26S rRNA gene revealed that three representative strains (ZO03-5(T), CA06-8 and ME06-9) constitute a taxon related to, but distinct from, Zygosaccharomyces machadoi. Similarity between the 26S rRNA gene domain D1/D2 sequence of the three isolates and Z. machadoi was 97.9%; moreover, the morphological characteristics and the physiological behaviour also supported recognition of a novel taxon of osmophilic non-psychrophilic yeast showing a flower-like arrangement of budding cells that remain attached to each other. The name Zygosaccharomyces gambellarensis is proposed for the novel species, with ZO03-5(T) (=CBS 12191(T)=MUCL 53393(T)) as the type strain.
authors.affiliation: Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Torriani
authors.affiliation: Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy.
authors.forename: Marilinda
authors.initials: M
authors.lastname: Lorenzini
authors.affiliation: Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Salvetti
authors.affiliation: Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy.
authors.forename: Giovanna E
authors.initials: GE
authors.lastname: Felis
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D027843:Vitis / Q000378:metabolism / Q000382:microbiology; D014920:Wine / Q000382:microbiology*; D020068:Zygosaccharomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S