title: Emergence of linezolid-resistant mutants in a susceptible-cell population of methicillin-resistant Staphylococcus aureus.
abstract: Methicillin-resistant Staphylococcus aureus with a MIC of linezolid of 4 μg/ml, isolated from a patient who had undergone unsuccessful linezolid therapy, yielded linezolid-resistant mutants in blood agar at 48 h of incubation. The resistant clones showed a MIC of linezolid ranging from 8 to 64 μg/ml and accumulated the T2500A mutation(s) of the rRNA genes. Emergence of these resistant clones appears to be facilitated by a cryptic mutation or mutations associated with chloramphenicol resistance.
authors.affiliation: Laboratory for Antimicrobial Agents, Kitasato University, 5-9-1 Shirokane, Minato-Ku, Tokyo 108-8641, Japan.
authors.forename: Yurika
authors.initials: Y
authors.lastname: Ikeda-Dantsuji
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hanaki
authors.forename: Taiji
authors.initials: T
authors.lastname: Nakae
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Takesue
authors.forename: Kazunori
authors.initials: K
authors.lastname: Tomono
authors.forename: Junichi
authors.initials: J
authors.lastname: Honda
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Mikamo
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Fukuchi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kaku
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Niki
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D000069349:Linezolid; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009154:Mutation; D023303:Oxazolidinones / Q000494:pharmacology*
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D023303:Oxazolidinones; D000069349:Linezolid