title: PGC-1α regulates translocated in liposarcoma activity: role in oxidative stress gene expression.
abstract: Translocated in liposarcoma (TLS) is a poorly characterized multifunctional protein involved in the genotoxic response. TLS regulates gene expression at several steps, including splicing and mRNA transport, possibly connecting transcriptional and posttranscriptional events.
  
  
  OBJECTIVE
  In this study we aimed to idenfity molecular targets and regulatory partners of TLS.
  
  
  RESULTS
  Here we report that TLS transcriptionally regulates the expression of oxidative stress protection genes. This regulation requires interaction with the transcriptional coactivator peroxisome proliferator activated receptor γ-coactivator 1α (PGC-1α), a master regulator of mitochondrial function that coordinately induces the expression of genes involved in detoxification of mitochondrial reactive oxygen species (ROS). Microarray gene expression analysis showed that TLS transcriptional activity is impaired in the absence of PGC-1α, and is thus largely dependent on PGC-1α.
  
  
  CONCLUSIONS
  These results suggest the existence of a regulatory circuit linking the control of ROS detoxification to the coordinated cross-talk between oxidative metabolism and the cellular response to genomic DNA damage.
authors.affiliation: Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Sánchez-Ramos
authors.forename: Alberto
authors.initials: A
authors.lastname: Tierrez
authors.forename: Oscar
authors.initials: O
authors.lastname: Fabregat-Andrés
authors.forename: Brigitte
authors.initials: B
authors.lastname: Wild
authors.forename: Fatima
authors.initials: F
authors.lastname: Sánchez-Cabo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Arduini
authors.forename: Ana
authors.initials: A
authors.lastname: Dopazo
authors.forename: María
authors.initials: M
authors.lastname: Monsalve
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004734:Energy Metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000235:genetics*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D034702:RNA-Binding Protein FUS / Q000235:genetics*; D015534:Trans-Activators / Q000502:physiology*; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques
chemical_list: D017931:DNA Primers; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D034702:RNA-Binding Protein FUS; D015534:Trans-Activators; D014157:Transcription Factors