title: Challenges and solutions for the identification of membrane proteins in non-model plants.
abstract: The workhorse for proteomics in non-model plants is classical two-dimensional electrophoresis, a combination of iso-electric focusing and SDS-PAGE. However, membrane proteins with multiple membrane spanning domains are hardly detected on classical 2-DE gels because of their low abundance and poor solubility in aqueous media. In the current review, solutions that have been proposed to handle these two problems in non-model plants are discussed. An overview of alternative techniques developed for membrane proteomics is provided together with a comparison of their strong and weak points. Subsequently, strengths and weaknesses of the different techniques and methods to evaluate the identification of membrane proteins are discussed. Finally, an overview of recent plant membrane proteome studies is provided with the used separation technique and the number of identified membrane proteins listed.
authors.affiliation: Laboratory of Tropical Crop Improvement, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 13, B-3001 Heverlee, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Vertommen
authors.forename: B
authors.initials: B
authors.lastname: Panis
authors.forename: R
authors.initials: R
authors.lastname: Swennen
authors.forename: S C
authors.initials: SC
authors.lastname: Carpentier
mesh_terms: D002458:Cell Fractionation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing / Q000379:methods; D008565:Membrane Proteins / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012995:Solubility
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins