title: Analysis of gene expression : northern blotting.
abstract: Messenger (m)RNAs represent the information-carrying intermediates for protein synthesis. Northern blotting gives a snapshot of the relative abundance of an RNA transcript at a set time point by measuring the steady-state levels of an RNA transcript. The steady-state level of a particular mRNA species is determined by its rate of production, degradation, and transport from the nucleus to cytoplasm. Northern blotting allows investigation and quantification of changes in gene expression.
authors.affiliation: Department of Clinical Ophthalmology, Institute of Ophthalmology, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Rousell
authors.forename: el-B
authors.initials: el-B
authors.lastname: Haddad