title: The integrin-binding motif RGDS induces protein tyrosine phosphorylation without activation in Bufo arenarum (Amphibia) oocytes.
abstract: Integrins are cell adhesion molecules that are thought to be involved in sperm-oocyte interaction. Nevertheless, their function in mammalian fertilization is still controversial, as different species behave differently. In amphibians, their role is mainly supported by Xenopus laevis studies, where RGDS peptide induces oocyte activation. We recently provided evidence suggesting the presence and involvement of integrins in the interaction of the oocyte plasma membrane (PM) with sperm in the amphibian Bufo arenarum. In order to understand the role of integrin homologs in oocytes and their possible contribution to egg activation mechanisms, we examined the presence of integrin subunits and the effect of RGDS peptide on oocytes and during fertilization. Western blot studies detected integrin subunits α5, αV and β1 in oocytes. In sperm, we could detect only the αV integrin subunit. We found that RGDS peptide was unable to elicit egg activation or MAPK dephosphorylation, but can induce reversible inhibition of fertilization. A similar partial inhibition was produced by an anti-β1 integrin antibody. Using an anti-phosphotyrosine antibody we found major changes in phosphotyrosine-containing proteins in egg extracts minutes after fertilization. Cytosol and PMs isolated from oocytes and fertilized eggs showed additional fertilization-induced phosphorylated proteins. Some of these were also present in cytosol and PMs from RGDS-treated oocytes (partially mimicking fertilization). These findings suggest that B. arenarum fertilization involves integrins (e.g. β1 subunit) as adhesion proteins. Our data support the view that RGDS-binding receptors may function as signaling receptors in B. arenarum oocytes, but integrin engagement by RGDS is not sufficient for oocyte activation.
authors.affiliation: División Biología del Desarrollo, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-UNR), Área Biología, Departamento de Ciencias Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
authors.forename: Valeria S
authors.initials: VS
authors.lastname: Mouguelar
authors.forename: Marcelo O
authors.initials: MO
authors.lastname: Cabada
authors.forename: Gabriela
authors.initials: G
authors.lastname: Coux
mesh_terms: D029845:Amphibian Proteins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002022:Bufo arenarum / Q000378:metabolism*; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D016023:Integrins / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects* / Q000201:enzymology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D013084:Sperm-Ovum Interactions / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014443:Tyrosine
chemical_list: D029845:Amphibian Proteins; D016023:Integrins; D009842:Oligopeptides; D014443:Tyrosine; C046617:arginyl-glycyl-aspartyl-serine; D048049:Extracellular Signal-Regulated MAP Kinases