title: Disruption of Sema3A expression causes abnormal neural projection in heavy oil exposed Japanese flounder larvae.
abstract: It has been well known that oil spills cause serious problems in the aquatic organisms. In particular, some species of teleosts, which develop on the sea surface thought to be affected by heavy oil (HO). During the embryogenesis, the nervous system is constructed. Therefore, it is important to study the toxicological effects of HO on the developing neurons. We exposed HO to eggs of Japanese flounder (Paralichthys olivaceus) and investigated the neural disorder. In larvae exposed by HO at the concentration of 8.75 mg/L, the facial and lateral line nerves partially entered into the incorrect region and the bundle was defasciculated. Furthermore, in the HO-exposed larvae, Sema3A, a kind of axon guidance molecule, was broadly expressed in second pharyngeal arch, a target region of facial nerve. Taken together, we suggested the possibility that the abnormal expression of Sema3A affected by HO exposure causes disruption of facial nerve scaffolding.
authors.affiliation: Center for Marine Environmental Studies, Graduate School of Science and Engineering, Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan. kawa@sci.ehime-u.ac.jp
authors.forename: Masahumi
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Kouta
authors.initials: K
authors.lastname: Irie
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Murakami
authors.forename: Kei
authors.initials: K
authors.lastname: Nakayama
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects / Q000378:metabolism; D005432:Flounder; D005786:Gene Expression Regulation / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D009420:Nervous System / Q000378:metabolism; D009421:Nervous System Malformations / Q000139:chemically induced* / Q000196:embryology / Q000378:metabolism; D010578:Petroleum / Q000633:toxicity*; D040121:Semaphorin-3A / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010578:Petroleum; D040121:Semaphorin-3A; D014874:Water Pollutants, Chemical