title: Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation.
abstract: In general, the action of intracellular second messengers, 3':5'-cyclic adenosine monophosphate (cAMP) and 3':5'-cyclic guanosine monophosphate (cGMP), are terminated by cyclic nucleotide phosphodiesterase (PDE). In most mammalian tissues, PDE exists in multiple forms that differ in subcellular localization, catalytic, regulatory, and immunological properties. One of these forms of enzyme is activated by Ca(2+) and calmodulin (CaM) and has a lower apparent K ( m ) for cGMP than for cAMP and higher V (max) for cAMP than for cGMP. This CaM-dependent cyclic nucleotide phosphodiesterase (CaMPDE) is widely distributed among mammalian tissues and other eukaryotes. CaMPDE is one of the most intensively studied and best characterized PDEs.
authors.affiliation: Department of Pathology, Saskatoon Cancer Centre, College of Medicine, University of Saskatchewan, Sakatoon, Saskatchewan, Canada.
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Sharma
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002147:Calmodulin / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose / Q000379:methods*; D000242:Cyclic AMP / Q000378:metabolism; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1 / Q000302:isolation & purification / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives
chemical_list: D000180:Acrylic Resins; D002147:Calmodulin; D007527:Isoenzymes; C037239:Sephacryl Superfine; C047264:sepharose CL 6B; D012685:Sepharose; D000242:Cyclic AMP; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1