title: [Influence of penicillin and streptomycin on gene expression of extracellular secretion from human umbilical cord tissue derived mesenchymal stem cells in vitro].
abstract: The study was aimed to investigate the influence of penicillin and streptomycin on proliferation, apoptosis and extracellular secretion (ECS) produced from human umbilical cord derived mesenchymal stem cells (MSC). MSC were isolated from umbilical cord tissue, then the immunotyping, multipotent differentiation and proliferation of these cells were assayed by cytometry, cytochemistry and MTT respectively. The expressions of ECS and apoptosis-related genes (bcl-2, bax) were detected by quantitative RT-PCR. The results showed that the phenotype of these cells matched with the characteristics of MSC. Penicillin and streptomycin of low concentrations promoted MSC proliferation, with the most effective concentration of 100 U/ml. Expressions of ECS cultured in addition of penicillin and streptomycin were down-regulated. Furthermore, apoptosis-related factor (bcl-2/bax) expression levels in low concentrations penicillin and streptomycin groups were higher than that in the control group. It is concluded that low concentrations penicillin and streptomycin can promote the proliferation and reduce the apoptotic rate, but high dose can inhibit the ECS component expression of MSC.
authors.affiliation: Department of Gynecology and Obstetrics, Shandong University Affiliated Provincial Hospital, Jinan 250005, Shandong Province, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Shi
authors.forename: Xiao
authors.initials: X
authors.lastname: Xing
authors.forename: Da-Kun
authors.initials: DK
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Dong
authors.initials: D
authors.lastname: Li
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D010406:Penicillins / Q000494:pharmacology*; D013307:Streptomycin / Q000494:pharmacology*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D010406:Penicillins; D013307:Streptomycin