title: The establishment of a library screening method based on yeast two-hybrid system and its use to determine the potential interactions of liver proteins in ayu, Plecoglossus altivelis.
abstract: Knowledge of specific protein-protein interaction (PPI) is an important component in understanding biological processes and regulatory mechanisms. A library to library screening method (LLS) was established based on yeast two-hybrid (YTH) system in this research, and applied to study the PPIs in ayu liver. In total, 23 out of 55 interaction pairs were found positive through phenotypic identification, with a positive rate of 41.8%. Of the 11 unique PPIs, 9 interactions including FGB/FGG, CaM/Spna2, C9/Apo-AI-1, α₂M/Ft, RPL10/RPL5, C8α/C9, FGG/Apo-AI-1, LECT2/Tf, and Apo-AI-2/C9 were previously reported. The other two PPIs including FGG/CLR and Wap65/C3 are novel, and in vitro co-immunoprecipitation (co-IP) experiments further confirmed these interactions. FGG/CLR interaction might play a role in regulating the inflammatory response. The interaction between Wap65 and C3 hints that Wap65 might function through the complement activation pathways when microbial infection occurs.
authors.affiliation: Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, PR China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Shi
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: C H
authors.initials: CH
authors.lastname: Li
authors.forename: H Y
authors.initials: HY
authors.lastname: Yang
authors.forename: X J
authors.initials: XJ
authors.lastname: Lu
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000235:genetics*; D015723:Gene Library; D008099:Liver / Q000502:physiology*; D036441:Osmeriformes / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D029941:Fish Proteins