title: [Anti-tumor effect of alcohol extract of Stellera chamaejasme in vitro].
abstract: OBJECTIVE
  To observe the anti-tumor effect of alcohol extract of Stellera chamaejasme-AESC, AESC-1 and AESC-2 in vitro.
  
  
  METHODS
  Inhibition rates of AESC, AESC-1, AESC-2 on five tumor cells (A549, NCI-H157, NCI-H460, BEL-7402 and SK-HEP-1 cell lines) were observed and IC50, GI50, TGI50 and LC50 were calculated with the methods of MTT and SRB.
  
  
  RESULTS
  AESC, AESC-1, AESC-2 have inhibitory effect on all of 5 cell lines and have a certain time-dependent nature. On 72 hours, inhibition rates of 100, 200 mg x L(-1) of various extracts on each cell line (except A549) were between 64.82% and 92.27%. IC50 of AESC and AESC-2 on NCI-H460 and BEL-7402 cell lines is less than 20 mg x L(-1), IC50 of AESC-1 on SK-HEP-1 cell line is less than 20 mg x L(-1). The mean GI50, TGI and LC50 of AESC, AESC-1, AESC-2 on sensitive cell lines were less than 40, 90, 150 mg x L(-1) respectively.
  
  
  CONCLUSIONS
  AESC, AESC-1 and AESC-2 all have significant anti-tumor effect and have the different sensitivity to different cell lines; AESC-2 has the similar anti-tumor manner to the AESC and the anti-tumor active fraction of AESC may be mainly present in the AESC-2.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. liuxiaoni888@yahoo.com.cn
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: An
authors.initials: A
authors.lastname: Liu
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000431:Ethanol; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D008175:Lung Neoplasms / Q000188:drug therapy; D029645:Thymelaeaceae / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D000431:Ethanol