title: Antitumor effect of Croatian propolis as a consequence of diverse sex-related dihydropyrimidine dehydrogenase (DPD) protein expression.
abstract: The aim of this study was to detect the antitumor properties of Croatian propolis in BALB/c male and female mice injected with 4T1 mammary carcinoma. Furthermore, the gender-dependence of this effect and the possible involvement of combined effect of propolis and 5-Fluorouracil (5FU) on dihydropyrimidine dehydrogenase (DPD) transcriptional and translational level, were determined. In combination with 5FU propolis treatment induced gender-related effects. The results of the study revealed that pretreatment of mice with propolis combined with 5FU treatment prolonged the suppressive effect of 5FU on tumor growth and reduced the number of metastasis only in male mice. Only males pretreated with propolis prior to 5FU administration had decreased DPD protein level indicating higher sensitivity to 5FU. Thus, benefitial effects of propolis in male tumor-bearing mice treated with 5FU might be explained by increased sensitivity to 5FU as the result of translationally downregulated DPD.
authors.affiliation: Division of Molecular Medicine, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia. ssoboc@irb.hr
authors.forename: Sandra
authors.initials: S
authors.lastname: Sobočanec
authors.forename: Tihomir
authors.initials: T
authors.lastname: Balog
authors.forename: Ana
authors.initials: A
authors.lastname: Šariċ
authors.forename: Željka
authors.initials: Ž
authors.lastname: Mačak-Šafranko
authors.forename: Marina
authors.initials: M
authors.lastname: Štroser
authors.forename: Kamelija
authors.initials: K
authors.lastname: Žarković
authors.forename: Neven
authors.initials: N
authors.lastname: Žarković
authors.forename: Ranko
authors.initials: R
authors.lastname: Stojković
authors.forename: Siniša
authors.initials: S
authors.lastname: Ivanković
authors.forename: Tatjana
authors.initials: T
authors.lastname: Marotti
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000235:genetics / Q000378:metabolism*; D004359:Drug Therapy, Combination; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D008113:Liver Neoplasms / Q000188:drug therapy / Q000556:secondary; D008175:Lung Neoplasms / Q000188:drug therapy / Q000556:secondary; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000188:drug therapy; D011429:Propolis / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011429:Propolis; D042943:Dihydrouracil Dehydrogenase (NADP); D005472:Fluorouracil