title: Modulation of TNFSF expression in lymphoid tissue inducer cells by dendritic cells activated with Toll-like receptor ligands.
abstract: Toll-like receptors (TLRs), which recognize structurally conserved components among pathogens, are mainly expressed by antigen-presenting cells such as dendritic cells (DCs), B cells, and macrophages. Recognition through TLRs triggers innate immune responses and influences antigen-specific adaptive immune responses. Although studies on the expression and functions of TLRs in antigen-presenting cells have been extensively reported, studies in lymphoid tissue inducer (LTi) cells have been limited. In this study, we observed that LTi cells expressed TLR2 and TLR4 mRNA as well as TLR2 protein and upregulated OX40L, CD30L, and TRANCE expression after stimulation with the TLR2 ligand zymosan or TLR4 ligand LPS. The expression of tumor necrosis factor superfamily (TNFSF) members was significantly upregulated when cells were cocultured with DCs, suggesting that upregulated TNFSF expression may contribute to antigen-specific adaptive immune responses.
authors.affiliation: Department of Bioinformatics and Life Science, The College of Natural Science, Soongsil University, Seoul 156-743, Korea.
authors.forename: Sinsuk
authors.initials: S
authors.lastname: Han
authors.forename: Jihye
authors.initials: J
authors.lastname: Koo
authors.forename: Jingyu
authors.initials: J
authors.lastname: Bae
authors.forename: Soochan
authors.initials: S
authors.lastname: Kim
authors.forename: Song
authors.initials: S
authors.lastname: Baik
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
mesh_terms: D000818:Animals; D053285:CD30 Ligand / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D053278:OX40 Ligand / Q000235:genetics / Q000378:metabolism; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D048069:Tumor Necrosis Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D015054:Zymosan / Q000494:pharmacology*
chemical_list: D053285:CD30 Ligand; D008024:Ligands; D008070:Lipopolysaccharides; D053278:OX40 Ligand; D053245:RANK Ligand; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D048069:Tumor Necrosis Factors; D015054:Zymosan