title: A novel rapid and continuous procedure for large-scale purification of magnetosomes from Magnetospirillum gryphiswaldense.
abstract: A new rapid and continuous procedure was developed for purifying magnetosomes from Magnetospirillum gryphiswaldense MSR-1 cells on a large scale. The procedure included these steps: disruption of cells with a high-pressure homogeniser, isolation of magnetosomes with a continuous magnetism isolation system accompanied by low-power ultrasonication and urea treatment, removal of adsorbed and surface proteins with proteinase K, removal of nucleic acids with electro-elution, and replacement of the PBS buffer with distilled water by a magnetically stirred system. The purified magnetosomes were stored at -20 °C after lyophilized and treated with γ-rays. The time required for purification was reduced from 20-30 to 2-5 days. Evaluation of the purity of the resulting magnetosomes was carried out with SDS-PAGE, PCR, and Fourier-transform infrared spectroscopy. The overall data suggest that the method presented here is a simple, rapid, continuous, and highly efficient procedure for large-scale purification of magnetosomes.
authors.affiliation: State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jilun
authors.initials: J
authors.lastname: Li
mesh_terms: D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D055944:Magnetosomes / Q000737:chemistry* / Q000378:metabolism; D042201:Magnetospirillum / Q000737:chemistry* / Q000378:metabolism