title: Evolution of promoter affinity for transcription factors in the human lineage.
abstract: Changes in gene regulation are believed to play an important role in the evolution of animals. It has been suggested that changes in cis-regulatory regions are responsible for many or most of the anatomical and behavioral differences between humans and apes. However, the study of the evolution of cis-regulatory regions is made problematic by the degeneracy of transcription factor (TF) binding sites and the shuffling of their positions. In this work, we use the predicted total affinity of a promoter for a large collection of TFs as the basis for studying the evolution of cis-regulatory regions in mammals. We introduce the human specificity of a promoter, measuring the divergence between the affinity profile of a human promoter and its orthologous promoters in other mammals. The promoters of genes involved in functional categories such as neural processes and signal transduction, among others, have higher human specificity compared with the rest of the genome. Clustering of the human-specific affinities (HSAs) of neural genes reveals patterns of promoter evolution associated with functional categories such as synaptic transmission and brain development and to diseases such as bipolar disorder and autism.
authors.affiliation: Department of Genetics, Biology and Biochemistry, Molecular Biotechnology Center, University of Turin, Turin, Italy.
authors.forename: Ivan
authors.initials: I
authors.lastname: Molineris
authors.forename: Elena
authors.initials: E
authors.lastname: Grassi
authors.forename: Ugo
authors.initials: U
authors.lastname: Ala
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Di Cunto
authors.forename: Paolo
authors.initials: P
authors.lastname: Provero
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002415:Cats; D002417:Cattle; D016000:Cluster Analysis; D004251:DNA Transposable Elements / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D004285:Dogs; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D011323:Primates; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D014157:Transcription Factors