title: Graphene platform for hairpin-DNA-based impedimetric genosensing.
abstract: There is enormous need for sensitive and selective detection of single nucleotide polymorphism of a DNA strand as this issue is related to many major diseases and disorders, such as Parkinson's and Alzheimer's disease. To achieve sensitivity and selectivity of the detection, a highly sensitive transducer of the signal with high surface area is required. In this work we employ a graphene platform to combine the sensitivity of electrochemical impedance spectroscopy with the high selectivity of hairpin-shaped DNA probes for the rapid detection of single nucleotide polymorphism correlated to the development of Alzheimer's disease. We investigate the influence of various graphene platforms consisting of different numbers of same-sized graphene layers. We believe that our findings are an important step toward highly sensitive and selective sensing architectures.
authors.affiliation: Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bonanni
authors.forename: Martin
authors.initials: M
authors.lastname: Pumera
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D055029:Inverted Repeat Sequences / Q000235:genetics; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D006108:Graphite; D004247:DNA