title: Preparation of Double-Stranded RNA for Drosophila RNA Interference (RNAi).
abstract: INTRODUCTIONRNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. It has been used in our laboratory to phenocopy a series of known mutations in Drosophila, including twist, engrailed, daughterless, Dmef2, and, to a lesser extent, white in the adult eye. This protocol describes the preparation of dsRNA by in vitro transcription of complementary strands of a cloned DNA fragment that codes for all or a portion of the gene of interest, followed by annealing of the transcribed RNA.
authors.forename: Leonie
authors.initials: L
authors.lastname: Misquitta
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Paterson