title: Current physical and SDS extraction methods do not efficiently remove exosporium proteins from Bacillus anthracis spores.
abstract: Biochemical studies of the outermost spore layers of the Bacillus cereus family are hindered by difficulties in efficient dispersal of the external spore layers and difficulties in dissociating protein complexes that comprise the exosporium layer. Detergent and physical methods have been utilized to disrupt the exosporium layer. Herein we compare commonly used SDS extraction buffers used to extract spore proteins and demonstrate the incomplete extractability of the exosporium layer by these methods. Sonication and bead beating methods for exosporium layer removal were also examined. A combination of genetic and physical methods is the most effective for isolating proteins found in the spore exosporium.
authors.affiliation: Department of Veterinary Pathobiology, Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Thompson
authors.forename: Jana M
authors.initials: JM
authors.lastname: Binkley
authors.forename: George C
authors.initials: GC
authors.lastname: Stewart
mesh_terms: D001408:Bacillus anthracis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013010:Sonication / Q000379:methods*; D013171:Spores, Bacterial / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate