title: The interplay of DNA binding, ATP hydrolysis and helicase activities of the archaeal MCM helicase.
abstract: The MCM (minichromosome maintenance) proteins of archaea are widely believed to be the replicative DNA helicase of these organisms. Most archaea possess a single MCM orthologue that forms homo-multimeric assemblies with a single hexamer believed to be the active form. In the present study we characterize the roles of highly conserved residues in the ATPase domain of the MCM of the hyperthermophilic archaeon Sulfolobus solfataricus. Our results identify a potential conduit for communicating DNA-binding information to the ATPase active site.
authors.affiliation: Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK.
authors.forename: Li Phing
authors.initials: LP
authors.lastname: Liew
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Bell
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation / Q000502:physiology; D006868:Hydrolysis; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D026341:Minichromosome Maintenance 1 Protein / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D048229:Sulfolobus solfataricus
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; D026161:MADS Domain Proteins; D026341:Minichromosome Maintenance 1 Protein; D000255:Adenosine Triphosphate; D004265:DNA Helicases