title: Biased three-dimensional cell migration and collagen matrix modification.
abstract: Various tumours can be resected even for cure with complete removal. Surgical excision with a wide margin to ensure complete removal has therefore been suggested as the primary treatment for such lesions. The histological examination of the three-dimensional (3D) excision margins (3D histology) constitutes an important part of the quality control mechanisms in tumour surgery. Understanding histologically relevant properties of the constituents of the microenvironment in tumours and the circumferential portion of non-lesional tissue is therefore critical. Accompanied by the increasing availability of high performance computers in recent decades, there has been a strong movement aiming at the development of mathematical models whose implementations allow in silico simulations of biological reaction networks. Due to its relevance in numerous biological and pathological processes, there have been various attempts to model biased migration of single cells. The model introduced in this paper plays a prominent role insofar as it covers the under-represented 3D case. Moreover, it is uniformly formulated for both two and three dimensions. The velocity of each cell is characterised by a generalised Langevin equation, a stochastic differential equation, where chemotaxis as well as contact guidance are considered to simulate selected aspects of interactions between carcinoma cell groups and fibroblast-like cells. Algorithmic and numeric aspects of the developed equations are detailed in this paper and the results of the simulations are illustrated in different manners to emphasise specific features. A simple test scenario as well as a geometry based on segmentation data of a real histological slide has served for verification of the software. The resulting morphologies closely resemble that of desmoplastic stromal reaction readily identifiable in histological slides of infiltrating carcinoma, and the images can be interpreted in the context of 3D histology.
authors.affiliation: Saarland University, Faculty 6 - Mathematics and Computer Science, POB 151150, 66041 Saarbrücken, Germany. groh@num.uni-sb.de
authors.forename: A
authors.initials: A
authors.lastname: Groh
authors.forename: M
authors.initials: M
authors.lastname: Wagner
mesh_terms: D002465:Cell Movement / Q000502:physiology*; D005109:Extracellular Matrix / Q000473:pathology*; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D008954:Models, Biological; D009369:Neoplasms / Q000473:pathology*; D009716:Numerical Analysis, Computer-Assisted; D013269:Stochastic Processes