title: Matrix metalloproteinase expression in sheep with listerial meningoencephalitis.
abstract: Matrix metalloproteinases (MMPs) have been implicated in the pathogenesis of several central nervous system (CNS) diseases. In this study, we investigated the presence of Listeria monocytogenes antigens and detected the expression of MMP-9 and MMP-7 in the brains of 22 sheep with clinical signs and histopathological findings characteristic of listerial meningoencephalitis. Archived sections from the brainstem, cerebrum, and cerebellum were stained for immunohistochemistry. L. monocytogenes antigens were located mainly in the cytoplasm of neutrophils and some macrophages and/or extracellularly within microabscesses of the brainstem. MMP-9 was mainly immunolocalised in the endothelial cells, microglial cells, and neurons especially in inflammatory areas. MMP-7 immunoreactivity was detected in perivascular cuffs, microglial cells, and only a few neurons. Overall, immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of encephalitic listeriosis caused by L. monocytogenes, and MMP-9 and MMP-7 may contribute to the pathogenesis of listerial meningoencephalitis.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Yüzüncü Yıl University, 65080 Kampus, Van, Turkey. fatmasayn@hotmail.com
authors.forename: Fatma
authors.initials: F
authors.lastname: İlhan
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Ulusoy
authors.forename: Mehmet
authors.initials: M
authors.lastname: Halıgür
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology / Q000382:microbiology / Q000473:pathology; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008584:Meningitis, Listeria / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology
chemical_list: D020782:Matrix Metalloproteinases; D020783:Matrix Metalloproteinase 7; D020780:Matrix Metalloproteinase 9