title: CO2 exacerbates oxygen toxicity.
abstract: Reactive oxygen species (ROS) are harmful because they can oxidize biological macromolecules. We show here that atmospheric CO(2) (concentration range studied: 40-1,000 p.p.m.) increases death rates due to H(2)O(2) stress in Escherichia coli in a dose-specific manner. This effect is correlated with an increase in H(2)O(2)-induced mutagenesis and, as shown by 8-oxo-guanine determinations in cells, DNA base oxidation rates. Moreover, the survival of mutants that are sensitive to aerobic conditions (Hpx(-) dps and recA fur), presumably because of their inability to tolerate ROS, seems to depend on CO(2) concentration. Thus, CO(2) exacerbates ROS toxicity by increasing oxidative cellular lesions.
authors.affiliation: Aix Marseille Université, Laboratoire de Chimie Bactérienne (UPR 9043), Institut de Microbiologie de la Méditerranée (IFR88), CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ezraty
authors.forename: Maïalène
authors.initials: M
authors.lastname: Chabalier
authors.forename: Adrien
authors.initials: A
authors.lastname: Ducret
authors.forename: Etienne
authors.initials: E
authors.lastname: Maisonneuve
authors.forename: Sam
authors.initials: S
authors.lastname: Dukan
mesh_terms: D002245:Carbon Dioxide / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D016296:Mutagenesis / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide