title: A novel lipase-based stationary phase in liquid chromatography.
abstract: In this paper, a new chiral stationary phase (CSP) based on Candida antarctica lipase B (CALB) bounding to the surface of macroporous silica gel was developed and its stereoselectivity in enantioseparation and asymmetrical hydrolysis was evaluated. Three CALB-based HPLC columns with different amounts of enzyme immobilized were prepared by employing the immobilization method, namely "in batch". In this technique two chromatographic supports epoxy silica and aminopropyl silica were considered. This novel CSP was proven capable of hydrolyzing chiral esters asymmetrically as bioreactor and separating several aromatic alcohols and diniconazole enantiomers.
authors.affiliation: Department of Applied Chemistry, China Agricultural University, No. 2 Yuanmingyuan Western Road, Beijing 100094, PR China.
authors.forename: Zeying
authors.initials: Z
authors.lastname: He
authors.forename: Chunguang
authors.initials: C
authors.lastname: Lv
authors.forename: Xinxin
authors.initials: X
authors.lastname: Fan
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000438:Alcohols / Q000737:chemistry; D019149:Bioreactors; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D058428:Silica Gel / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism; D014230:Triazoles / Q000737:chemistry
chemical_list: D000438:Alcohols; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D014230:Triazoles; D058428:Silica Gel; D012822:Silicon Dioxide; D008049:Lipase; C431349:lipase B, Candida antarctica; C049346:diniconazole