title: Effect of training load structure on purine metabolism in middle-distance runners.
abstract: There are no studies analyzing the effect of training loads on purine metabolism during long training periods.
  
  
  OBJECTIVE
  The study's purpose was to evaluate the effect of training load changes and subsequent detraining on purine metabolism in middle-distance runners during a 1-yr cycle.
  
  
  METHODS
  In four characteristic points of the training cycle, loads assigned to five intensity zones, pre- and postexercise plasma hypoxanthine (Hx) and uric acid, and erythrocyte Hx-guanine phosphoribosyltransferase (HGPRT) activity were determined in 11 male middle-distance runners at the national level, practicing competitive sport for 8.1 ± 0.3 yr and with a mean age of 22.3 ± 0.7 yr, body mass of 73.0 ± 3.4 kg, and body height of 180 ± 2.2 cm.
  
  
  RESULTS
  In the competition phase (CP), training loads in aerobic compensation and threshold zones decreased by 65.4% and by 20.5%, respectively. At the same time, anaerobic training loads increased by 132.5% in the VO(2max) zone and by 74.6% in the lactic acid tolerance zone. Postexercise Hx decreased significantly in CP by 6.2 μmol·L(-1). and increased in the transition phase (TP) by 17.4 μmol·L(-1). Both pre- and postexercise HGPRT activity increased significantly in CP by 9.3 nmol·mg(-1)·h(-1). and by 4.9 nmol·mg(-1)·h(-1). , respectively, and decreased significantly in TP by 10.6 nmol·mg(-1)·h(-1). and by 12.0 nmol·mg(-1)·h(-1). , respectively. A significant uric acid increase of 54 μmol·L(-1). was revealed merely in TP.
  
  
  CONCLUSIONS
  The effect of anaerobic training on purine metabolism is significant despite of a very short total duration of anaerobic loads. Elevated preexercise HGPRT activity in CP suggests adaptation changes consisting in a "permanent readiness" for purine salvage. The detraining in TP leads to reverse adaptation changes. Probably, plasma Hx concentration and erythrocyte HGPRT activity may be considered as a useful measure of training status.
authors.affiliation: Department of Athletics, University School of Physical Education, Poznań, Poland. jacekzielinski@wp.pl
authors.forename: Jacek
authors.initials: J
authors.lastname: Zieliński
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kusy
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Rychlewski
mesh_terms: D056352:Athletes; D004912:Erythrocytes / Q000201:enzymology / Q000502:physiology; D015444:Exercise; D006801:Humans; D019271:Hypoxanthine / Q000097:blood; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology; D011687:Purines / Q000378:metabolism*; D012420:Running / Q000502:physiology*; D014527:Uric Acid / Q000097:blood; D055815:Young Adult
chemical_list: D011687:Purines; D014527:Uric Acid; D019271:Hypoxanthine; D007041:Hypoxanthine Phosphoribosyltransferase