title: Detection of Listeria monocytogenes in pork and beef using the VIDAS® LMO2 automated enzyme linked immunoassay method.
abstract: Listeria (L.) monocytogenes, a foodborne pathogen, is known to be a possible contaminant of foods during production and processing. Samples (n=985) of raw meat and by-products obtained from beef and pork were first screened by the VIDAS system for the presence of Listeria spp., followed by testing for the presence of L. monocytogenes. Positive L. monocytogenes results were confirmed by plating on selective agars: 14% of the samples were positive for Listeria and 4% tested positive for L. monocytogenes, of which 3% were confirmed on selective agars. In by-products (17%) the contamination with listeriae was higher than in meat cuts (10%). Only samples strongly positive for Listeria spp. by VIDAS were positive for L. monocytogenes. Overall, the prevalence of L. monocytogenes in beef and pork samples was rather low in comparison to most previous studies. The VIDAS system was shown to be a suitable method for screening out Listeria-negative samples; the main advantage being a markedly reduced assay time.
authors.affiliation: Institute of Food Science, Faculty of Veterinary Medicine, Ludwig-Maximilian-University Munich, Schönleutnerstraße 8, D-85764 Oberschleißheim, Germany. meyer@lmhyg.vetmed.uni-muenchen.de
authors.forename: Cornelia
authors.initials: C
authors.lastname: Meyer
authors.forename: Maria
authors.initials: M
authors.lastname: Fredriksson-Ahomaa
authors.forename: Brigitte
authors.initials: B
authors.lastname: Sperner
authors.forename: Erwin
authors.initials: E
authors.lastname: Märtlbauer
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005513:Food Inspection / Q000379:methods*; D005516:Food Microbiology; D005858:Germany; D006321:Heart / Q000382:microbiology; D007220:Industrial Waste; D007668:Kidney / Q000382:microbiology; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008099:Liver / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D008462:Meat-Packing Industry; D034421:Sus scrofa; D014059:Tongue / Q000382:microbiology
chemical_list: D007220:Industrial Waste