title: The chitinolytic activities of Streptomyces sp. TH-11.
abstract: Chitin is an abundant biopolymer composed of units of N-acetyl-D-glucosamine linked by β-1,4 glycosidic bonds. Chitin is the main component of the shells of mollusks, the cell wall of fungi and yeast and of the exoskeleton of crustaceans and insects. The degradation of chitin is catalyzed by chitinases that occur in a wide range of organisms. Among them, the chitinases from microorganisms are extremely important for the degradation and recycling of the carbon and nitrogen trapped in the large amount of insoluble chitin in nature. Streptomyces sp. TH-11 was isolated from the sediment of the Tou-Chien River, Taiwan. The chitinolytic enzyme activities were detected using a rapid in-gel detection method from the cell-free preparation of the culture medium of TH-11. The chitinolytic enzyme activity during prolonged liquid culturing was also analyzed by direct measurement of the chitin consumption. Decomposition of the exoskeleton of shrimps was demonstrated using electron microscopy and atomic force microscopy.
authors.affiliation: General Education Center, Ta Hwa Institute of Technology, HsinChu 307, Taiwan; E-Mail: chi@thit.edu.tw.
authors.forename: Kim-Chi
authors.initials: KC
authors.lastname: Hoang
authors.forename: Tzu-Hsuan
authors.initials: TH
authors.lastname: Lai
authors.forename: Chung-Sheng
authors.initials: CS
authors.lastname: Lin
authors.forename: Ying-Tsong
authors.initials: YT
authors.lastname: Chen
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Liau
mesh_terms: D060105:Animal Shells / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism / Q000502:physiology*; D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism / Q000502:physiology*; D018625:Microscopy, Atomic Force; D013302:Streptomyces / Q000201:enzymology* / Q000302:isolation & purification; D013499:Surface Properties
chemical_list: D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases