title: Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array.
abstract: We report a portable lensless on-chip microscope that can achieve <1 µm resolution over a wide field-of-view of ∼ 24 mm(2) without the use of any mechanical scanning. This compact on-chip microscope weighs ∼ 95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.
authors.affiliation: Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA. bishara@ucla.edu ozcan@ucla.edu
authors.forename: Waheb
authors.initials: W
authors.lastname: Bishara
authors.forename: Uzair
authors.initials: U
authors.lastname: Sikora
authors.forename: Onur
authors.initials: O
authors.lastname: Mudanyali
authors.forename: Ting-Wei
authors.initials: TW
authors.lastname: Su
authors.forename: Oguzhan
authors.initials: O
authors.lastname: Yaglidere
authors.forename: Shirley
authors.initials: S
authors.lastname: Luckhart
authors.forename: Aydogan
authors.initials: A
authors.lastname: Ozcan
mesh_terms: D004912:Erythrocytes / Q000469:parasitology; D005898:Glass / Q000737:chemistry; D006696:Holography / Q000295:instrumentation*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation*; D055100:Optical Fibers; D010963:Plasmodium falciparum / Q000502:physiology