title: MicroRNA-16 targets zyxin and promotes cell motility in human laryngeal carcinoma cell line HEp-2.
abstract: MicroRNAs are small noncoding RNAs that negatively regulate target mRNAs at the posttranscription level. Here, we show that miR-16 is upregulated in human laryngeal carcinoma tissues. Inhibition of miR-16 in HEp-2 laryngeal cancer cell line could suppress cell migration and enhance cell-cell adhesion. Subsequently, zyxin, whose expression is negatively regulated by miR-16, is confirmed to be a direct target gene of miR-16. Furthermore, overexpression of zyxin could also restrain cell movement and enhance cell-cell adhesion. The study of miR-16 and its target zyxin will shed light on diagnosis and therapy of laryngeal cancer.
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Renjie
authors.initials: R
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Tian
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D014170:Transformation, Genetic; D015854:Up-Regulation; D060589:Zyxin
chemical_list: D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; D006023:Glycoproteins; D035683:MicroRNAs; C092842:ZYX protein, human; D060589:Zyxin