title: Self-assembled virus-like particles from rotavirus structural protein VP6 for targeted drug delivery.
abstract: Proteins of viral capsid may self-assemble into virus-like particles (VLPs) that can find many biomedical applications such as platform for drug delivery. In this paper, we describe preparation of VLPs by self-assembly of VP6, a rotavirus capsid protein that was chemically conjugated with doxorubicin (DOX), an anticancer drug. VP6 was first highly expressed in E. Coli, followed by purification and renaturation. DOX was then covalently attached to VP6 to form DOX-VP6 (DVP6) conjugates, which were subsequently self-assembled into VLPs under appropriate condition. Next, lactobionic acid (LA) was chemically linked to the surface of the VLPs. We demonstrated that the aforementioned nanosystem shows specific targeting to hepatoma cell line HepG2. The chemically functionalized VLPs, a kind of biological nanoparticles with excellent biocompatibility and biodegradability, can be prepared in large scale from E. Coli through our method, which may find practical applications in biomedicine.
authors.affiliation: Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
authors.forename: Qinghuan
authors.initials: Q
authors.lastname: Zhao
authors.forename: Weihong
authors.initials: W
authors.lastname: Chen
authors.forename: Yuanding
authors.initials: Y
authors.lastname: Chen
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000737:chemistry; D037263:Asialoglycoprotein Receptor / Q000378:metabolism; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004187:Disaccharides / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D009994:Osmolar Concentration; D020673:Protein Renaturation; D021381:Protein Transport; D012401:Rotavirus; D013379:Substrate Specificity
chemical_list: D000956:Antigens, Viral; D000970:Antineoplastic Agents; D037263:Asialoglycoprotein Receptor; D036022:Capsid Proteins; D004187:Disaccharides; D004337:Drug Carriers; C061332:VP6 protein, Rotavirus; C005608:lactobionic acid; D004317:Doxorubicin