title: Molecular cloning, expression, and characterization of cyclophilin A from Clonorchis sinensis.
abstract: This study described the recognization, cloning, and recombinant expression of cyclophilin A-like gene from Clonorchis sinensis adult complementary DNA library (CsCyPA) and its expression and secretion in adult. Western blotting demonstrated the recombinant CsCyPA could be recognized by sera of clonorchiasis patients and a sole protein of the same size in the excretory-secretory antigens of in vitro cultured adult could be recognized by antiserum raised against the recombinant CsCyPA. Immunohistochemistry demonstrated that the CsCyPA was secreted in scattered vesicles from subtegumental parenchyma cells to the surface of tegument and mainly released from the tegument. ELISA showed the serum levels of IgG against CsCyPA in clonorchiasis patients negatively correlated with worm loads. This study suggested that C. sinensis adult in biliary ducts could release CsCyPA without signal peptide through nonclassical secretory pathway into the liver and might play a role in inflammation and biliary epithelium proliferation and adenomatoid hyperplasia.
authors.affiliation: Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wu
authors.forename: Jiajia
authors.initials: J
authors.lastname: Chen
authors.forename: Suxiang
authors.initials: S
authors.lastname: Zeng
authors.forename: Zhaoping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Wenjia
authors.initials: W
authors.lastname: Gan
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
authors.forename: Xuchu
authors.initials: X
authors.lastname: Hu
mesh_terms: D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D015153:Blotting, Western; D003001:Cloning, Molecular; D003004:Clonorchis sinensis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D021984:Cyclophilin A / Q000235:genetics* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D021984:Cyclophilin A