title: Comparative study of the bactericidal effects of 5-aminolevulinic acid with blue and red light on Propionibacterium acnes.
abstract: Propionibacterium acnes naturally produces endogenous porphyrins that are composed of coproporphyrin III (CPIII) and protoporphyrin IX (PpIX). Red light alone and photodynamic therapy (PDT) improve acne vulgaris clinically, but there remains a paucity of quantitative data that directly examine the bactericidal effects that result from PDT on P. acnes itself in vitro. The purpose of this study was to measure the difference of bactericidal effects of 5-aminolevulinic acid (ALA)-PDT with red and blue light on P. acnes. P. acnes were cultured under anaerobic conditions and divided into two groups (ALA-treated group and control group), and were then illuminated with blue (415 nm) and red (635 nm) lights using a light-emitting diode (LED). The cultured P. acnes were killed with both blue and red LED light illumination. The efficacy increased with larger doses of light and a greater number of consecutive illuminations. We demonstrated that red light phototherapy was less effective for the eradication of P. acnes than blue light phototherapy without the addition of ALA. However, pretreatment with ALA could enhance markedly the efficacy of red light phototherapy.
authors.affiliation: Department of Dermatology, Chonnam National University Medical School, Gwangju, Korea.
authors.forename: Myoung-Soon
authors.initials: MS
authors.lastname: Choi
authors.forename: Sook Jung
authors.initials: SJ
authors.lastname: Yun
authors.forename: Hee Ju
authors.initials: HJ
authors.lastname: Beom
authors.forename: Hyoung Ryun
authors.initials: HR
authors.lastname: Park
authors.forename: Jee-Bum
authors.initials: JB
authors.lastname: Lee
mesh_terms: D000152:Acne Vulgaris / Q000188:drug therapy* / Q000382:microbiology; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003306:Coproporphyrins / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy* / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity / Q000528:radiation effects*; D011524:Protoporphyrins / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003306:Coproporphyrins; D017319:Photosensitizing Agents; D011524:Protoporphyrins; C026971:coproporphyrin III; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX