title: miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells.
abstract: Reactive oxygen species (ROS) produced in macrophages is critical for microbial killing, but they also take part in inflammation and antigen presentation functions. MicroRNAs (miRNAs) are endogenous regulators of gene expression, and they can control immune responses. To dissect the complex nature of ROS-mediated effects in macrophages, we sought to characterize miRNAs that are responsive to oxidative stress-induced with hydrogen peroxide (H(2)O(2)) in the mouse macrophage cell line, RAW 264.7. We have identified a set of unique miRNAs that are differentially expressed in response to H(2)O(2). These include miR-27a*, miR-27b*, miR-29b*, miR-24-2*, and miR-21*, all of which were downregulated except for miR-21*. By using luciferase reporter vector containing nuclear factor-kB (NF-kB) response elements, we demonstrate that overexpression of miR-27b* suppresses lipopolysaccharide-induced activation of NF-kB in RAW 264.7 cells. Our data suggest that macrophage functions can be regulated by oxidative stress-responsive miRNAs by modulating the NF-kB pathway.
authors.affiliation: School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
authors.forename: Sivasubramani
authors.initials: S
authors.lastname: Thulasingam
authors.forename: Chandirasegaran
authors.initials: C
authors.lastname: Massilamany
authors.forename: Arunakumar
authors.initials: A
authors.lastname: Gangaplara
authors.forename: Hongjiu
authors.initials: H
authors.lastname: Dai
authors.forename: Shahlo
authors.initials: S
authors.lastname: Yarbaeva
authors.forename: Sakthivel
authors.initials: S
authors.lastname: Subramaniam
authors.forename: Jean-Jack
authors.initials: JJ
authors.lastname: Riethoven
authors.forename: James
authors.initials: J
authors.lastname: Eudy
authors.forename: Marjorie
authors.initials: M
authors.lastname: Lou
authors.forename: Jay
authors.initials: J
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002460:Cell Line; D051379:Mice; D035683:MicroRNAs / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D016328:NF-kappa B