title: Inhibition of Taura syndrome virus replication in Litopenaeus vannamei through silencing the LvRab7 gene using double-stranded RNA.
abstract: Taura syndrome virus (TSV) is a major cause of high mortality in Pacific white shrimp (Litopenaeus vannamei, Lv). Previously, silencing of Penaeus monodon Rab7 (PmRab7) by injecting double-stranded RNA corresponding to PmRab7 (dsRNA-PmRab7) prevented white spot syndrome virus or yellow head virus infection. Rab7 is proposed to be involved in intracellular trafficking of the viruses. This study aimed to investigate whether knockdown of Rab7 in L. vannamei by dsRNA-PmRab7 could inhibit replication of TSV. RNA interference (RNAi) technology using dsRNA targeting the LvRab7 gene was used to silence the mRNA expression of LvRab7. The silencing of the LvRab7 gene inhibited TSV replication dramatically when compared to groups receiving dsRNA-GFP or NaCl. This is the first demonstration that dsRNA targeting the endogenous shrimp gene LvRab7 strongly reduces TSV replication. It provides further evidence that LvRab7 is involved in the endosomal trafficking pathway of viruses infecting penaeid shrimp.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University (Salaya Campus), 25/25 Phutthamonthon 4 Rd. Salaya, Phutthamonthon district, Nakhon Pathom, 73170, Thailand. mbcov@mahidol.ac.th
authors.forename: Chalermporn
authors.initials: C
authors.lastname: Ongvarrasopone
authors.forename: Pipop
authors.initials: P
authors.lastname: Saejia
authors.forename: Mayuree
authors.initials: M
authors.lastname: Chanasakulniyom
authors.forename: Sakol
authors.initials: S
authors.lastname: Panyim
mesh_terms: D000818:Animals; D056629:Dicistroviridae / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation; D033561:Penaeidae / Q000821:virology*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D000090802:rab7 GTP-Binding Proteins
chemical_list: D012330:RNA, Double-Stranded; D000090802:rab7 GTP-Binding Proteins; D020691:rab GTP-Binding Proteins