title: [Expression and clinical significance of ZO-1 in patients with non-small cell lung cancer].
abstract: OBJECTIVE
  It has been proven that the expression of ZO-1 (zonula occluden-1) was correlated with the growth and metastasis of cancer. The aim of this study is to investigate the relationship between ZO-1 gene expression and clinicopathologic parameters and the survival rates in the patients with non-small cell lung cancer (NSCLC).
  
  
  METHODS
  The expression levels of ZO-1 gene in 101 patients with NSCLC and lung tissues of 61 patients with benign lung disease were detected by real-time PCR, Western blot and immunohistochemical techniques.
  
  
  RESULTS
  There was a significant statistic difference of ZO-1 mRNA expression levels between carcinoma group and control group (P < 0.01). In carcinoma group, adjacent group and control group, ZO-1 protein expression levels were with significantly statistic difference between each other (P < 0.01 or P < 0.05). There was a significantly statistic difference between ZO-1 mRNA expression levels in survival during 2 years follow-up group and death during 2 years follow-up group (t=-5.61, P < 0.01).
  
  
  CONCLUSIONS
  ZO-1 is a valuable predictive marker for NSCLC patients.
authors.affiliation: Laboratory of Molecular Biology, Zhoushan Hospital, 316004 Zhoushan, China.
authors.forename: Yekai
authors.initials: Y
authors.lastname: Wang
authors.forename: Jihang
authors.initials: J
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Zeng
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Shiquan
authors.initials: S
authors.lastname: Zhou
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D013534:Survival; D062826:Zonula Occludens-1 Protein
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein