title: The predominant expression of hepatocyte nuclear factor 4α (HNF4α) in thyroid transcription factor-1 (TTF-1)-negative pulmonary adenocarcinoma.
abstract: OBJECTIVE
  To investigate TTF-1-negative pulmonary adenocarcinoma, focusing upon mucin production and the expression of hepatocyte nuclear factor-4α (HNF4α).
  
  
  METHODS
  Two hundred and sixty-two cases of pulmonary adenocarcinoma were examined histologically and immunohistochemically; TTF-1 was expressed in 222 cases (84.7%), and 40 cases (15.3%) were negative. Among TTF-1-negative cases there were 31 mucinous-type tumours, and HNF4α, MUC5AC and MUC2 were expressed in 34 cases (85%), 29 cases (72.5%) and four cases (10%), respectively. In contrast, their expression was rare in TTF-1-positive tumours. A statistically inverse correlation was confirmed between the expression of TTF-1 and that of HNF4α and MUC5AC.
  
  
  CONCLUSIONS
  Most TTF-1-negative pulmonary adenocarcinomas are mucinous lesions with the predominant expression of HNF4α and MUC5AC.
authors.affiliation: Division of Cellular and Molecular Pathology, Niigata University Graduate School of Medical and Dental Sciences, Niigata University Hospital, Niigata, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kunii
authors.forename: Shuying
authors.initials: S
authors.lastname: Jiang
authors.forename: Go
authors.initials: G
authors.lastname: Hasegawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Hajime
authors.initials: H
authors.lastname: Umezu
authors.forename: Takehiro
authors.initials: T
authors.lastname: Watanabe
authors.forename: Masanori
authors.initials: M
authors.lastname: Tsuchida
authors.forename: Takehisa
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sasai
authors.forename: Makoto
authors.initials: M
authors.lastname: Naito
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D051557:Hepatocyte Nuclear Factor 4 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051557:Hepatocyte Nuclear Factor 4; C524678:MUC5AC protein, human; D055271:Mucin 5AC; C000614412:NKX2-1 protein, human; D009687:Nuclear Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors