title: Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region.
abstract: We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Egawa
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Koide
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D012825:Silicon / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014966:Xanthenes / Q000737:chemistry; D001616:beta-Galactosidase / Q000737:chemistry / Q000378:metabolism
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D014966:Xanthenes; D001616:beta-Galactosidase; D012825:Silicon