title: Use of soybean vinasses as a germinant medium for a Geobacillus stearothermophilus ATCC 7953 sterilization biological indicator.
abstract: A novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media in the production of a steam sterilization biological indicator (BI). Various recovery media were developed using soybean or sugarcane molasses and vinasse to prepare a self-contained BI. Media performance was evaluated by viability and heat resistance (D(121 °C) value) according to regulatory standards. A medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) produced the results, with D(121 °C)=2.9±0.5 min and Usk=12.7±2.1 min. The addition of 0.8% (w/v) yeast extract improved the germination of heat-damaged spores. The pH variation from 6.0 to 7.3 resulted in a gradual increase in the D(121 °C) value. The absence of calcium chloride resulted in a decrease in germination, while no significant differences were observed with starch addition. Soybean vinasses may thus be used as the main component of a culture medium to substitute for commercial media in the production of self-contained biological indicators. The use of ethanol production waste in this biotechnological process realized a reliable performance, minimized the environmental impact, and decreased BI production costs while producing a high quality product.
authors.affiliation: Centro de Produção e Pesquisa de Imunobiológicos, Secretaria de Estado da Saúde do Paraná, Av. São Roque, 716, CEP 83.302-200 Piraquara, Paraná, Brazil. rdlugokenski@gmail.com
authors.forename: Regina E F
authors.initials: RE
authors.lastname: Dlugokenski
authors.forename: Sandra R B R
authors.initials: SR
authors.lastname: Sella
authors.forename: Belquis P
authors.initials: BP
authors.lastname: Guizelini
authors.forename: Luciana P S
authors.initials: LP
authors.lastname: Vandenberghe
authors.forename: Adenise L
authors.initials: AL
authors.lastname: Woiciechowski
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Soccol
authors.forename: João C
authors.initials: JC
authors.lastname: Minozzo
mesh_terms: D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000254:growth & development* / Q000378:metabolism; D006358:Hot Temperature; D007220:Industrial Waste; D050296:Microbial Viability; D008965:Molasses; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism; D013171:Spores, Bacterial / Q000254:growth & development; D013242:Sterilization
chemical_list: D003470:Culture Media; D007220:Industrial Waste; D000431:Ethanol