title: Multiplex PCR for Detection of the Prothrombin 3'-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation.
abstract: Prothrombotic evaluation of patients with a history-and in particular a family history-of venous thromboembolic disease is becoming increasingly important as our understanding of the molecular abnormalities that underlie this clinical disorder increases. A recently described G→A polymorphism at position 20210 in the 3'-untranslated region of the prothrombin gene (F2 3'-UTR) has been found to be associated with an increased risk of venous thrombotic disease. In the Leiden Thrombophilia Study (LETS), the prevalence of carriers of the 20210 A allele in the healthy population was 2.3%, among patients with a single objectively proven DVT 6.2% and in a selected group of patients with a personal and family history of venous thrombosis 18%.
authors.affiliation: Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free Hospital School of Medicine, London, UK.
authors.forename: G
authors.initials: G
authors.lastname: Mellars
authors.forename: P V
authors.initials: PV
authors.lastname: Jenkins
authors.forename: D J
authors.initials: DJ
authors.lastname: Perry