title: Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS).
abstract: In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.
authors.affiliation: Institute of Physical Chemisty, Friedrich Schiller University, Jena, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: März
authors.forename: Bettina
authors.initials: B
authors.lastname: Mönch
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.forename: Michael
authors.initials: M
authors.lastname: Kiehntopf
authors.forename: Thomas
authors.initials: T
authors.lastname: Henkel
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D015122:Mercaptopurine / Q000031:analogs & derivatives / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: C010240:6-methylthiopurine; D015122:Mercaptopurine; D008780:Methyltransferases; C022745:thiopurine methyltransferase