title: Effect of hyperbaric oxygen therapy on bone prefabrication in rats.
abstract: OBJECTIVE
  This experimental study aimed to create a prefabricated vascularized bone graft using the interconnected porous coralline hydroxyapatite ceramic by combining vascular bundle implantation, mesenchymal stem cells, and hyperbaric oxygen therapy (HBOT) administration in a rat model.
  
  
  METHODS
  Forty-five male Sprague-Dawley rats were divided into three groups, each containing 15 rats. The hydroxyapatite ceramics were vascularized by the superficial inferior epigastric artery and vein in all groups. These vessels passed through the hole of the hydroxyapatite blocks. In Group 2, mesenchymal stem cells were administered into the hydroxyapatite. In Group 3, both mesenchymal stem cells and HBOT were administered. The presence and density of any new bone formation and neovascularization were evaluated by radiography, microangiography, scintigraphy, biochemical analysis, and histomorphometry.
  
  
  RESULTS
  Neovascularization and bone formation were significantly greater in Group 3, in which both mesenchymal stem cells and HBOT were applied, than the other groups.
  
  
  CONCLUSIONS
  HBOT enhances neovascularization and osteogenesis, thus HBOT can provide optimal and faster prefabrication of a vascularized bone graft.
authors.affiliation: GATA Haydarpaşa Training Hospital, Department of Plastic and Reconstructive Surgery and Burn Unit, İstanbul, Turkey. drcsever@hotmail.com
authors.forename: Celalettin
authors.initials: C
authors.lastname: Sever
authors.forename: Fatih
authors.initials: F
authors.lastname: Uygur
authors.forename: Yalçın
authors.initials: Y
authors.lastname: Külahçı
authors.forename: Gamze
authors.initials: G
authors.lastname: Torun Köse
authors.forename: Muammer
authors.initials: M
authors.lastname: Urhan
authors.forename: Zafer
authors.initials: Z
authors.lastname: Küçükodacı
authors.forename: Günalp
authors.initials: G
authors.lastname: Uzun
authors.forename: Osman
authors.initials: O
authors.lastname: Ipçioğlu
authors.forename: Tuncer
authors.initials: T
authors.lastname: Caycı
mesh_terms: D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones / Q000098:blood supply* / Q000000981:diagnostic imaging; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000379:methods*; D020007:Implants, Experimental; D008297:Male; D018919:Neovascularization, Physiologic; D010012:Osteogenesis / Q000502:physiology*; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*