title: Comparison of complement fixation test, competitive ELISA and LppQ ELISA with post-mortem findings in the diagnosis of contagious bovine pleuropneumonia (CBPP).
abstract: The complement fixation test (CFT), the c-ELISA and an indirect LppQ ELISA were compared to post-mortem (PM) inspection for the diagnosis of contagious bovine pleuropneumonia (CBPP). Sera from 797 cattle in the CBPP affected area of Kazungula, Zambia and 202 sera from Lusaka, Zambia, a CBPP-free area were used. The clinical history of CBPP was recorded and all the cattle from Kazungula were slaughtered and PM inspections conducted. The prevalence of CBPP in Kazungula was 67.5% (95%CI 67.2%, 70.8%), 52.6% (95%CI 49.2%, 56.2%), 59.0% (95%CI 55.5%, 62.4%) and 44.4% (95%CI 41.0%, 47.9%) using PM inspection, CFT, c-ELISA and LppQ ELISA, respectively. Three of the 202 negative control animals tested positive on the c-ELISA although they were from a known CBPP negative zone. In this study, the c-ELISA was more sensitive in detecting cattle with lesions in the chronic stage than any other test whilst the CFT detected more during the onset stage. No single serological test could detect all stages of CBPP infection, therefore the use of more than one test is advised.
authors.affiliation: Central Veterinary Research Institute, Ministry of Livestock and Fisheries Development, Lusaka, Zambia.
authors.forename: Geofrey
authors.initials: G
authors.lastname: Muuka
authors.forename: Bernard Mudenda
authors.initials: BM
authors.lastname: Hang'ombe
authors.forename: King Shimumbo
authors.initials: KS
authors.lastname: Nalubamba
authors.forename: Swithine
authors.initials: S
authors.lastname: Kabilika
authors.forename: Lucas
authors.initials: L
authors.lastname: Mwambazi
authors.forename: John Bwalya
authors.initials: JB
authors.lastname: Muma
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001344:Autopsy / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D003168:Complement Fixation Tests / Q000379:methods* / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary*; D009176:Mycoplasma mycoides / Q000276:immunology*; D011002:Pleuropneumonia, Contagious / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D016036:Seroepidemiologic Studies; D015024:Zambia / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial