title: [Isolation and identification of impurities from raw material of clindamycin phosphate].
abstract: Four impurities were isolated from raw material of clindamycin phosphate (CP), and their structures have been determined. LC-MS was used to determine the molecular weights of the impurities in the raw material of CP. Reversed-phase preparative HPLC was used to prepare them, and their chemical structures were identified by HR-MS and NMR. The four unknown impurities were determined as clindamycin-B-phosphate (1), clindamycin-2,4-diphosphate (2), 3',6'-dehydro clindamycin phosphate (3), epi-clindamycin phosphate (4). Impurity 1 has been included in BP and EP, while 2, 3 and 4 have not. The impurities 2, 3, 4 are first separated from raw material of CP.
authors.affiliation: Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China. liyue.cn@126.com
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.forename: Shu-Zeng
authors.initials: SZ
authors.lastname: Chen
authors.forename: Qiu-Shi
authors.initials: QS
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Qin
authors.forename: Ling-Yue
authors.initials: LY
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D002981:Clindamycin / Q000031:analogs & derivatives* / Q000737:chemistry; D004340:Drug Contamination; D015394:Molecular Structure; D008970:Molecular Weight; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin; C007084:clindamycin phosphate