title: Radiation-induced ICAM-1 expression via TGF-β1 pathway on human umbilical vein endothelial cells; comparison between X-ray and carbon-ion beam irradiation.
abstract: Adhesion of inflammatory cells to endothelial cells is considered to be involved in the process of radiation-induced damage and fibrosis. Intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-beta1 (TGF-β1) are thought to play important roles in this process. In this study, radiation-induced ICAM-1 expression on endothelial cells was investigated with the use of an inhibitor of TGF-β1 receptor kinase (SB431542) and the effects of X-ray and carbon-ion beam were compared. Cell cultures of human umbilical vein endothelial cells (HUVE cells) were incubated with TGF-β1 and irradiated with 140 KV X-ray. Next, HUVE cells were irradiated with X-ray and 220 MeV carbon-ion beam with or without SB431542. Immunofluorescence analysis was used to quantify ICAM-1 expression. The expression of ICAM-1 on HUVE cells was significantly increased by the stimulation with TGF-β1. Expression of ICAM-1 was increased by X-ray and carbon-ion beam irradiation and decreased significantly with SB431542 after both irradiations. The expression of ICAM-1 by 2 Gy of carbon-ion beam irradiation was 6.7 fold higher than that of non-irradiated cells, while 5 Gy of X-ray irradiation increased the expression of ICAM-1 by 2.5 fold. According to ICAM-1 expression, the effect of carbon-ion beam irradiation was about 2.2, 4.4 and 5.0 times greater than that of the same doses of X-ray irradiation (1, 2 and 5 Gy, respectively). The present results suggested that radiation-induced ICAM-1 expression on HUVE cells was, at least partially, regulated by TGF-β1. Carbon-ion beam induced significantly higher ICAM-1 expression than X-ray.
authors.affiliation: Department of Radiation Oncology, Gunma University Graduate School of Medicine, Maebashi.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kiyohara
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ishizaki
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katoh
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamada
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ohno
authors.forename: Takeo
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
mesh_terms: D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation / Q000502:physiology / Q000528:radiation effects; D042783:Endothelial Cells / Q000378:metabolism* / Q000528:radiation effects*; D020450:Heavy Ions; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D011829:Radiation Dosage; D015398:Signal Transduction / Q000502:physiology* / Q000528:radiation effects; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014471:Umbilical Veins / Q000378:metabolism / Q000528:radiation effects; D014965:X-Rays
chemical_list: D002250:Carbon Radioisotopes; D053773:Transforming Growth Factor beta1; D018799:Intercellular Adhesion Molecule-1