title: Involvement of viral envelope GP2 in Ebola virus entry into cells expressing the macrophage galactose-type C-type lectin.
abstract: Ebola virus (EBOV) infection is initiated by the interaction of the viral surface envelope glycoprotein (GP) with the binding sites on target cells. Differences in the mortality among different species of the Ebola viruses, i.e., Zaire ebolavirus (ZEBOV) and Reston ebolavirus (REBOV), correspond to the in vitro infectivity of the pseudo-typed virus constructed with the GPs in cells expressing macrophage galactose-type calcium-type lectin (MGL/CD301). Through mutagenesis of GP2, the transmembrane-anchored subunit of GP, we found that residues 502-527 of the GP2 sequence determined the different infectivity between VSV-ZEBOV GP and -REBOV GP in MGL/CD301-expressing cells and a histidine residue at position 516 of ZEBOV GP2 appeared essential in the differential infectivity. These findings may provide a clue to clarify a molecular basis of different pathogenicity among EBOV species.
authors.affiliation: Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Usami
authors.forename: Keita
authors.initials: K
authors.lastname: Matsuno
authors.forename: Manabu
authors.initials: M
authors.lastname: Igarashi
authors.forename: Kaori
authors.initials: K
authors.lastname: Denda-Nagai
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Irimura
mesh_terms: D019943:Amino Acid Substitution; D045744:Cell Line, Tumor; D029043:Ebolavirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D057809:HEK293 Cells; D019142:Hemorrhagic Fever, Ebola / Q000378:metabolism / Q000821:virology*; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D016296:Mutagenesis; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D037181:Lectins, C-Type; C506225:MGL lectin, human; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus