title: FISH on Histological Sections.
abstract: IntroductionHere we describe fluorescence in situ hybridization (FISH) of DNA probes to histological sections, which allows the visualization of specific DNA targets (chromosome territories and their subregions) in the context of functional tissue organization. Separate protocols are provided for hybridization using paraffin-embedded tissue sections and for hybridization using vibratome or frozen sections. Pretreatment with heat or protease is necessary to allow unmasking of the target DNA and efficient penetration of reagents in the nuclei. Because the goal of the technique is to obtain data on the native 3D structure of the genome, close attention is paid to the preservation of nuclear morphology.
authors.affiliation: Ludwig-Maximilians University Munich, Department of Biology II, AG Thomas Cremer (Chair of Anthropology and Human Genetics), 82152 Martinsried-Planegg, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Solovei
authors.forename: Florian
authors.initials: F
authors.lastname: Grasser
authors.forename: Christian
authors.initials: C
authors.lastname: Lanctôt