title: Expression of sorting nexin 18 (SNX18) is dynamically regulated in developing spinal motor neurons.
abstract: The sorting nexin (SNX) family proteins, which contain a Phox homology (PX) domain, play crucial roles in regulating the intracellular membrane trafficking of the endocytic pathway. The proper coordination of this pathway is important for axonal elongation; however, little is known about the expression and intracellular dynamics of the SNX members during the formation of the nervous system. Here the authors found that SNX18, which belongs to the Src-homology-3-PX-Bin/Amphiphysin/Rvs domain-containing SNX subfamily, was specifically expressed in differentiating motor neurons in the chick and mouse embryonic spinal cord. The expression of SNX18 in embryonic spinal motor neurons was transient and was downregulated as the neurons matured. The authors further demonstrated that the localization of EGFP-SNX18 in growth cones was dynamically regulated and accumulated especially at areas in contact with permissive substrates. These findings collectively suggest that SNX18 may play an active role in axonal elongation.
authors.affiliation: Laboratory of Neuroscience, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Shiori
authors.initials: S
authors.lastname: Nakazawa
authors.forename: Naoya
authors.initials: N
authors.lastname: Gotoh
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Chiaki
authors.initials: C
authors.lastname: Murayama
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tohru
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002645:Chickens; D018507:Gene Expression Regulation, Developmental; D020439:Growth Cones / Q000378:metabolism; D051379:Mice; D009046:Motor Neurons / Q000378:metabolism*; D021381:Protein Transport; D058305:Sorting Nexins / Q000235:genetics* / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology
chemical_list: C524095:Snx19 protein, mouse; D058305:Sorting Nexins