title: A polygalacturonase-inhibiting protein with a role in pea defence against the cyst nematode Heterodera goettingiana.
abstract: A cDNA of 312 bp, similar to polygalacturonase-inhibiting proteins (PGIPs), was isolated by cDNA-amplified fragment length polymorphism (cDNA-AFLP) from pea roots infected with the cyst nematode Heterodera goettingiana. The deduced amino acid sequence obtained from the complete Pspgip1 coding sequence was very similar to PGIPs described from several other plant species, and was identical in both MG103738 and Progress 9 genotypes, resistant and susceptible to H. goettingiana, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) expression analysis revealed the differential regulation of the Pspgip1 gene in the two genotypes in response to wounding and nematode challenge. Mechanical wounding induced Pspgip1 expression in MG103738 within 8 h, but this response was delayed in Progress 9. In contrast, the response to nematode infection was more complex. The transcription of Pspgip1 was triggered rapidly in both genotypes, but the expression level returned to levels observed in uninfected plants more quickly in susceptible than in resistant roots. In addition, in situ hybridization showed that Pspgip1 was expressed in the cortical cells damaged as a result of nematode invasion in both genotypes. However, it was specifically localized in the cells bordering the nematode-induced syncytia in resistant roots. This suggests a role for this gene in counteracting nematode establishment inside the root.
authors.affiliation: Istituto per la Protezione delle Piante, CNR, 70126 Bari, Italy. p.veronico@ba.ipp.cnr.it
authors.forename: Pasqua
authors.initials: P
authors.lastname: Veronico
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Melillo
authors.forename: Concetta
authors.initials: C
authors.lastname: Saponaro
authors.forename: Paola
authors.initials: P
authors.lastname: Leonetti
authors.forename: Ernesto
authors.initials: E
authors.lastname: Picardi
authors.forename: John T
authors.initials: JT
authors.lastname: Jones
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D003517:Cyclopentanes / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018745:Genome, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000469:parasitology; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014431:Tylenchoidea / Q000187:drug effects / Q000502:physiology*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; C081479:PGIP protein, plant; D010940:Plant Proteins; D012333:RNA, Messenger; C072239:methyl jasmonate