title: Altered expression of Dscam in temporal lobe tissue from human and experimental animals.
abstract: Down syndrome cell adhesion molecule (Dscam) is a neural adhesion molecule that plays an essential role in the establishment of neural circuits. Considerable evidence suggests that Dscam is required for axon guidance and dendritic arborization. Our aim was to investigate the expression of Dscam in the temporal lobes of patients with intractable epilepsy (IE) and of experimental animals. In this study, we used immunohistochemistry, immunofluorescence, and western blotting to examine Dscam expression in thirty-five surgical samples from brains of IE patients and 15 control brain samples. We also measured the levels of Dscam during the entire epileptic process in a rat model of temporal lobe epilepsy. Dscam expression in IE patients was significantly higher compared with that in the controls. In addition, Dscam was also highly expressed in the rat brain during the different phases of the epileptic process. It is the first time to find abnormal expression of Dscam in the brain tissues in patients with IE. And this finding provides an experimental evidence for the study of neuronal circuit remodeling and synaptic plasticity in IE, furthermore, our results also suggest that Dscam may be involved in the generation and the development of IE.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Neurology, Jiangjin Central Hospital, Chongqing 402260, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Shen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Yumin
authors.initials: Y
authors.lastname: Pan
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Chengshan
authors.initials: C
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xi
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002648:Child; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000209:etiology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018721:Muscarinic Agonists / Q000633:toxicity; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009473:Neuronal Plasticity / Q000235:genetics; D010862:Pilocarpine / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000235:genetics; D013702:Temporal Lobe / Q000378:metabolism* / Q000503:physiopathology; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; C110833:DSCAM protein, human; C572419:DSCAM protein, rat; D018721:Muscarinic Agonists; D009419:Nerve Tissue Proteins; D010862:Pilocarpine