title: Potential effects of Cramoll 1,4 lectin on murine Schistosomiasis mansoni.
abstract: Cratylia mollis is a natural forage plant from the Northeast of Brazil. C. mollis seed lectin (Cramoll) containing molecular forms 1 and 4 (Cramoll 1,4) has shown anti-inflammatory and wound-healing activities. This work analyzed the effect of Cramoll 1,4 on experimental schistosomiasis in mice. Experimental groups (n=15/group) were composed of female albino Swiss mice, which were subcutaneously and caudally infected with Schistosoma mansoni (BH strain, 100 cercariae/mouse) and were treated with an intraperitoneal dose after infection as follows: (1) Cramoll 1,4 (50 mg kg(-1) single dose - after 40 days of infection), (2) Cramoll 1,4 (7 mg kg(-1) daily dose - for 7 days after infection) and control (untreated mice). Mice were sacrificed 8 weeks after infection and adult worms were recovered from the portal-hepatic system. Livers were fixed in 10% (v/v) formaldehyde/0.15M NaCl and tissue sections were processed for haematoxilin and Masson's trichrome stainings. Mice infected subcutaneously harboured no or very few worms and hence the effect of Cramoll 1,4 could not be assessed. Results (P≤0.05) were obtained with Cramoll 1,4 using the two treatments, with reduction of: egg excretion (79 and 80%), adult worm recovery (71 and 79%) and liver granulomas (40 and 73.5%) in relation to control. This study showed the potential anti-helminthic activity of Cramoll 1,4 when tested against Schistosomiasis mansoni infection in mice.
authors.affiliation: Laboratório de Imunogenética, Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ, Pernambuco, Brazil. crismout_melo@hotmail.com
authors.forename: Cristiane Moutinho Lagos de
authors.initials: CM
authors.lastname: Melo
authors.forename: Amanda Lucena Rosendo
authors.initials: AL
authors.lastname: de Lima
authors.forename: Eduardo Isidoro Carneiro
authors.initials: EI
authors.lastname: Beltrão
authors.forename: Carmelita C Bezerra
authors.initials: CC
authors.lastname: Cavalcanti
authors.forename: Mário Ribeiro
authors.initials: MR
authors.lastname: de Melo-Júnior
authors.forename: Silvia Maria L
authors.initials: SM
authors.lastname: Montenegro
authors.forename: Luana Cassandra B Barroso
authors.initials: LC
authors.lastname: Coelho
authors.forename: Maria Tereza dos Santos
authors.initials: MT
authors.lastname: Correia
authors.forename: Ana Maria dos Anjos
authors.initials: AM
authors.lastname: Carneiro-Leão
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage* / Q000302:isolation & purification; D001938:Brazil; D004195:Disease Models, Animal; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D007274:Injections, Intraperitoneal; D037102:Lectins / Q000008:administration & dosage* / Q000302:isolation & purification; D008099:Liver / Q000469:parasitology / Q000473:pathology; D051379:Mice; D010270:Parasite Egg Count; D011169:Portal Vein / Q000469:parasitology; D012550:Schistosoma mansoni / Q000187:drug effects*; D012555:Schistosomiasis mansoni / Q000188:drug therapy*; D012639:Seeds / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D037102:Lectins