title: Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination.
abstract: A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of C. elegans embryos are shown, and the capabilities and the limitations of the scheme are discussed.
authors.affiliation: University of Konstanz, Department of Chemistry, D-78457 Konstanz, Germany.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Martin
authors.initials: M
authors.lastname: Winterhalder
authors.forename: Romedi
authors.initials: R
authors.lastname: Selm
authors.forename: Andreas
authors.initials: A
authors.lastname: Zumbusch
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D018715:Microscopy, Video / Q000295:instrumentation*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000379:methods*