title: Biological reactivity of nanoparticles: mosaics from optical microscopy videos of giant lipid vesicles.
abstract: Emerging fields such as nanomedicine and nanotoxicology, demand new information on the effects of nanoparticles on biological membranes and lipid vesicles are suitable as an experimental model for bio-nano interaction studies. This paper describes image processing algorithms which stitch video sequences into mosaics and recording the shapes of thousands of lipid vesicles, which were used to assess the effect of CoFe(2)O(4) nanoparticles on the population of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine lipid vesicles. The applicability of this methodology for assessing the potential of engineered nanoparticles to affect morphological properties of lipid membranes is discussed.
authors.affiliation: University of Ljubljana, Faculty of Computer and Information Science, Trzaska 25, SI-1000, Ljubljana, Slovenia. jernej.zupanc@fri.uni-lj.si
authors.forename: Jernej
authors.initials: J
authors.lastname: Zupanc
authors.forename: Andrej
authors.initials: A
authors.lastname: Dobnikar
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobne
authors.forename: Janez
authors.initials: J
authors.lastname: Valant
authors.forename: Deniz
authors.initials: D
authors.lastname: Erdogmus
authors.forename: Erhan
authors.initials: E
authors.lastname: Bas
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods; D018715:Microscopy, Video / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D053835:Unilamellar Liposomes