title: Identification and characterization of a clam ferritin from Sinonovacula constricta.
abstract: Ferritin, a major iron storage protein of most living organisms, plays a crucial role in iron metabolism. Here we reported the isolation and characterization of a cDNA of ferritin gene from Sinonovacula constricta (denoted as ScFER). The full-length cDNA of ScFER was of 996 bp, consisting of a 5'-UTR of 120 bp, a 3'-UTR of 360 bp, and a complete open reading frame of 516 bp encoding a polypeptide with 171 amino acid residues. The predicted molecular mass of deduced amino acid of ScFER was 19.76 kDa and the theoretical pI was 5.07. Quantitative real-time PCR was employed to analyze the expression profiles of ScFER mRNA in muscle, mantle and visceral mass after iron exposure. The peak expression level of ScFER in the three tissues was 1.79-fold, 1.31-fold and 3.51-fold increases in muscle, mantle and visceral mass, respectively. The polyclonal antibodies generated from the recombinant product of ScFER could be specifically identified not only the recombinant product, but also the native protein from muscle. All these results strongly suggested that ScFER was involved in the iron metabolism regulation in S. constricta.
authors.affiliation: Faculty of Life Science and Biotechnology, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211, PR China. chli@yic.ac.cn
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xiurong
authors.initials: X
authors.lastname: Su
authors.forename: Taiwu
authors.initials: T
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D005293:Ferritins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014781:Viscera / Q000737:chemistry / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D012313:RNA; D005293:Ferritins; D007501:Iron