title: Effects of cell lysis treatments on the yield of coenzyme Q10 following Agrobacterium tumefaciens fermentation.
abstract: The yield of CoQ₁₀, an intracellular product extracted from Agrobacterium tumefaciens cells is dependent on the effectiveness of cell lysis post fermentation. Various cell lysis approaches are investigated, including ultrasound, repetitive freezing/thawing, grinding and acid-heat treatment. The acid-heat combination using hydrochloric acid is found the most effective in releasing CoQ₁₀, followed by lactic, sulfuric, phosphoric and oxalic acids. The most significant processing parameters, namely the ratio of acid solution volume and bacteria weight (A/B ratio), incubation temperature and reaction time, are optimized by using the central composite design with a quadratic regression model built by response surface methodology. The highest CoQ₁₀ yield at 1.518 mg/g dry cell is attained using hydrochloric acid (3 mol/L) under optimal A/B ratio, temperature and time at 10.8 mL/g, 84.2 °C and 35.3 min, respectively.
authors.affiliation: College of Food Science and Engineering, Northwest A and F University, Yangling Shaanxi, P. R. China 712100.
authors.lastname: Yuting Tian
authors.lastname: Tianli Yue
authors.lastname: Jinjin Pei
authors.lastname: Yahong Yuan
authors.lastname: Juhai Li
authors.forename: Y
authors.initials: Y
authors.lastname: Martin Lo
mesh_terms: D016960:Agrobacterium tumefaciens / Q000201:enzymology* / Q000254:growth & development; D018533:Biomass; D005285:Fermentation; D006851:Hydrochloric Acid / Q000378:metabolism; D013010:Sonication / Q000379:methods*; D013696:Temperature; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D014451:Ubiquinone; C024989:coenzyme Q10; D006851:Hydrochloric Acid