title: Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.
abstract: BACKGROUND
  The SMC5-6 protein complex is involved in the cellular response to DNA damage. It is composed of 6-8 polypeptides, of which Nse1, Nse3 and Nse4 form a tight sub-complex. MAGEG1, the mammalian ortholog of Nse3, is the founding member of the MAGE (melanoma-associated antigen) protein family and Nse4 is related to the EID (E1A-like inhibitor of differentiation) family of transcriptional repressors.
  
  
  RESULTS
  Using site-directed mutagenesis, protein-protein interaction analyses and molecular modelling, we have identified a conserved hydrophobic surface on the C-terminal domain of Nse3 that interacts with Nse4 and identified residues in its N-terminal domain that are essential for interaction with Nse1. We show that these interactions are conserved in the human orthologs. Furthermore, interaction of MAGEG1, the mammalian ortholog of Nse3, with NSE4b, one of the mammalian orthologs of Nse4, results in transcriptional co-activation of the nuclear receptor, steroidogenic factor 1 (SF1). In an examination of the evolutionary conservation of the Nse3-Nse4 interactions, we find that several MAGE proteins can interact with at least one of the NSE4/EID proteins.
  
  
  CONCLUSIONS
  We have found that, despite the evolutionary diversification of the MAGE family, the characteristic hydrophobic surface shared by all MAGE proteins from yeast to humans mediates its binding to NSE4/EID proteins. Our work provides new insights into the interactions, evolution and functions of the enigmatic MAGE proteins.
authors.affiliation: Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors.forename: Jessica J R
authors.initials: JJ
authors.lastname: Hudson
authors.forename: Katerina
authors.initials: K
authors.lastname: Bednarova
authors.forename: Lucie
authors.initials: L
authors.lastname: Kozakova
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liao
authors.forename: Marc
authors.initials: M
authors.lastname: Guerineau
authors.forename: Rita
authors.initials: R
authors.lastname: Colnaghi
authors.forename: Susanne
authors.initials: S
authors.lastname: Vidot
authors.forename: Jaromir
authors.initials: J
authors.lastname: Marek
authors.forename: Sreenivas R
authors.initials: SR
authors.lastname: Bathula
authors.forename: Alan R
authors.initials: AR
authors.lastname: Lehmann
authors.forename: Jan
authors.initials: J
authors.lastname: Palecek
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence / Q000502:physiology; D019143:Evolution, Molecular; D006801:Humans; D058950:Melanoma-Specific Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D025941:Protein Interaction Mapping; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017385:Sequence Homology
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C416925:EID1 protein, human; C072011:MAGEA1 protein, human; D058950:Melanoma-Specific Antigens; D046912:Multiprotein Complexes; C495159:Nse3 protein, S pombe; C493528:Nse4 protein, S pombe; D009687:Nuclear Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C486628:Smc5 protein, S pombe; C486630:smc6 protein, S pombe