title: Preparation, characterization, and in vitro evaluation of docetaxel-loaded poly(lactic acid)-poly(ethylene glycol) nanoparticles for parenteral drug delivery.
abstract: The aim of this study was to develop a suitable drug delivery system without Tween 80 and design docetaxel (DTX)-loaded PLA-PEG nanoparticles to improve solubility and decrease side effect of docetaxel. DTX-loaded PLA-PEG nanoparticles were prepared by modified solvent volatilixation method and characterized by particle size distribution, zeta potential and entrapment efficiency. The morphology of DTX loaded PLA-PEG nanoparticles was approximately spherical. The mean diameter and zeta potential of freeze-dried PLA-PEG nanoparticles were 205 +/- 8.1 nm and -24.17 +/- 2.20 mV, respectively. The average entrapment efficiency and drug loading of freeze-dried DTX-loaded PLA-PEG nanoparticles were 91.83 +/- 1.28% and 8.17 +/- 0.35%, respectively. The in vitro release showed that the release from DTX loaded PLA-PEG nanoparticles was slower than from Duopafei and followed the Weibull equation. Cytotoxicity of Duopafei and DTX loaded PLA-PEG nanoparticles was evaluated. Compared with Duopafei, DTX-loaded PLA-PEG nanoparticles showed similar cytotoxicity against three human cancer cell lines and lower toxicity on human normal hepatocellular HL-7702 cells. The apoptosis was detected and measured with Hoechst 33342 and AnnexinV-FITC kit. Compared with Duopafei, DTX-loaded PLA-PEG nanoparticles revealed similar cytotoxicity against A549 cells by inducing similar apoptosis. These results indicated that the PLA-PEG nanoparticles obtained in this study could potentially be exploited as a carrier without Tween 80, which improved drug solubility and decrease side effect of docetaxel.
authors.affiliation: School of Pharmaceutical Science, Shandong University Ji'nan 250012, China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Liu
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.forename: Cai
authors.initials: C
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000077143:Docetaxel; D016503:Drug Delivery Systems; D006801:Humans; D007263:Infusions, Parenteral; D020128:Inhibitory Concentration 50; D019344:Lactic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D043823:Taxoids / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D011091:Polyesters; D011108:Polymers; D043823:Taxoids; D000077143:Docetaxel; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide)