title: D(-)-lactic acid production by Leuconostoc mesenteroides B512 using different carbon and nitrogen sources.
abstract: Sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. The concentrations of these two components were further optimized using the Plackett-Burman design and response surface method. A second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/L would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/L, respectively. To validate the optimization of the medium composition, studies were carried out using the optimized conditions to confirm the result of the response surface analysis. After 48 h, lactic acid production using the shake-flask method was at 60.2 g/L.
authors.affiliation: Department of Biochemistry and Microbiology, Univ. Estadual Paulista-UNESP, Biological Sciences Institute, CEP 13506-900, Rio Claro, SP, Brazil.
authors.forename: Luciana Fontes
authors.initials: LF
authors.lastname: Coelho
authors.forename: Cristian J Bolner
authors.initials: CJ
authors.lastname: de Lima
authors.forename: Marcela Piassi
authors.initials: MP
authors.lastname: Bernardo
authors.forename: Jonas
authors.initials: J
authors.lastname: Contiero
mesh_terms: D000465:Algorithms; D002244:Carbon / Q000378:metabolism*; D002457:Cell Extracts; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D019344:Lactic Acid / Q000096:biosynthesis*; D007934:Leuconostoc / Q000254:growth & development / Q000378:metabolism; D015233:Models, Statistical; D008965:Molasses; D009584:Nitrogen / Q000378:metabolism*; D031786:Saccharum / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D019344:Lactic Acid; D002244:Carbon; D009584:Nitrogen