title: [The effect of culture supernatant from MSCs on the proliferation and expression of COX-2, MMP-2 in rat synovial cell line].
abstract: OBJECTIVE
  To investigate the effect of culture supernatant form mesenchymal stem cells (MSCs) on the proliferation of rat fibroblast-like synovial cell line RSC-364 and the expression of COX-2, MMP-2.
  
  
  METHODS
  After isolation and identification of MSCs, the effect of MSCs supernatant liquid (MSCs-SL) on the proliferation of RSC-364 and the expression of COX-2, MMP-2 were detected by MTT assay and RT-PCR.
  
  
  RESULTS
  MSCs-SL enhanced the proliferation of synoviocyte and elevated the expression of COX-2 and MMP-2 in synoviocytes (P < 0.05).
  
  
  CONCLUSIONS
  MSCs may influence the proliferation of synoviocytes by secreting soluble cytokines and altered the expression level of some enzymes in synoviocytes.
authors.affiliation: Institute of Clinical Immunology, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: He-jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Shu-yu
authors.initials: SY
authors.lastname: Liu
authors.forename: Pin
authors.initials: P
authors.lastname: Li
authors.forename: Xiang-xiong
authors.initials: XX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000166:cytology* / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat