title: [Effect of curcumin on JAK-STAT signaling pathway in hepatoma cell lines].
abstract: The effect of curcumin on JAK-STAT signaling pathway was investigated in hepatoma cell lines Huh7 and Hep3B. Curcumin inhibited cell proliferation and induced apoptosis of both cell lines, but Huh7 cells were more sensitive to curcumin than Hep3B cells. Curcumin (50 micromol x L(-1)) significantly increased phosphorylations of p38 (T180/Y182) and STAT-1 (S727) in Huh7 and Hep3B cells, and caused relocalization of phosphorylated-STAT-1 (Y701) from cytoplasm to nucleus in Hep3B cells. In addition, curcumin (25 and 50 micromol x L(-1)) dramatically suppressed the phosphorylation level of STAT-1 (Y701) and resulted in a significant reduction of nuclear phosphorylated-STAT-1 (Y701) in Huh7 cells.
authors.affiliation: Guangdong Pharmaceutical University, Guangzhou 510006, China. wwzss@163.com
authors.forename: Wei-Zhang
authors.initials: WZ
authors.lastname: Wang
authors.forename: Bi-Yu
authors.initials: BY
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Mao
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Mei
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D053612:Janus Kinases / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D010766:Phosphorylation; D010946:Plants, Medicinal / Q000737:chemistry; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D053612:Janus Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D003474:Curcumin