title: An in vitro model that recapitulates the epithelial to mesenchymal transition (EMT) in human breast cancer.
abstract: The epithelial to mesenchymal transition (EMT) is a developmental program in which epithelial cells down-regulate their cell-cell junctions, acquire spindle cell morphology and exhibit cellular motility. In human breast cancer, invasion into surrounding tissue is the first step in metastatic progression. Here, we devised an in vitro model using selected cell lines, which recapitulates many features of EMT as observed in human breast cancer. By comparing the gene expression profiles of claudin-low breast cancers with the experimental model, we identified a 9-gene signature characteristic of EMT. This signature was found to distinguish a series of breast cancer cell lines that have demonstrable, classical EMT hallmarks, including loss of E-cadherin protein and acquisition of N-cadherin and vimentin expression. We subsequently developed a three-dimensional model to recapitulate the process of EMT with these cell lines. The cells maintain epithelial morphology when encapsulated in a reconstituted basement membrane, but undergo spontaneous EMT and invade into surrounding collagen in the absence of exogenous cues. Collectively, this model of EMT in vitro reveals the behaviour of breast cancer cells beyond the basement membrane breach and recapitulates the in vivo context for further investigation into EMT and drugs that may interfere with it.
authors.affiliation: Breakthrough Breast Cancer Research Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom. elad.katz@ed.ac.uk
authors.forename: Elad
authors.initials: E
authors.lastname: Katz
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dubois-Marshall
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Sims
authors.forename: Philippe
authors.initials: P
authors.lastname: Gautier
authors.forename: Helen
authors.initials: H
authors.lastname: Caldwell
authors.forename: Richard R
authors.initials: RR
authors.lastname: Meehan
authors.forename: David J
authors.initials: DJ
authors.lastname: Harrison
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001485:Basement Membrane / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D057167:Claudins / Q000235:genetics / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism
chemical_list: D057167:Claudins; D014157:Transcription Factors; D003094:Collagen