title: Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy.
abstract: We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.
authors.affiliation: National Research Council of Canada, Steacie Institute for Molecular Sciences, Ottawa, Ontario, K1A 0R6 Canada. slepkov@gmail.com
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Slepkov
authors.forename: Andrew
authors.initials: A
authors.lastname: Ridsdale
authors.forename: Huei-Ning
authors.initials: HN
authors.lastname: Wan
authors.forename: Ming-Hao
authors.initials: MH
authors.lastname: Wang
authors.forename: Adrian F
authors.initials: AF
authors.lastname: Pegoraro
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Moffatt
authors.forename: John P
authors.initials: JP
authors.lastname: Pezacki
authors.forename: Fu-Jen
authors.initials: FJ
authors.lastname: Kao
authors.forename: Albert
authors.initials: A
authors.lastname: Stolow
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*