title: Equipment Setup for Fluorescence Imaging with One-Nanometer Accuracy (FIONA).
abstract: INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This article describes procedures for setting up the equipment necessary for FIONA and achieving total internal reflection (TIR).
authors.affiliation: Biophysics Center and Physics Department, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Selvin
authors.forename: Tyler
authors.initials: T
authors.lastname: Lougheed
authors.forename: Melinda
authors.initials: M
authors.lastname: Tonks Hoffman
authors.forename: Hyokeun
authors.initials: H
authors.lastname: Park
authors.forename: Hamza
authors.initials: H
authors.lastname: Balci
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Blehm
authors.forename: Erdal
authors.initials: E
authors.lastname: Toprak