title: Oxidative stress-related responses of Bifidobacterium longum subsp. longum BBMN68 at the proteomic level after exposure to oxygen.
abstract: Bifidobacterium longum subsp. longum BBMN68, an anaerobic probiotic isolated from healthy centenarian faeces, shows low oxygen (3 %, v/v) tolerance. To understand the effects of oxidative stress and the mechanisms protecting against it in this strain, a proteomic approach was taken to analyse changes in the cellular protein profiles of BBMN68 under the following oxygen-stress conditions. Mid-exponential phase BBMN68 cells grown in MRS broth at 37 °C were exposed to 3 % O(2) for 1 h (I) or 9 h (II), and stationary phase cells were subjected to 3 % O(2) for 1 h (III). Respective controls were grown under identical conditions but were not exposed to O(2). A total of 51 spots with significant changes after exposure to oxygen were identified, including the oxidative stress-protective proteins alkyl hydroperoxide reductase C22 (AhpC) and pyridine nucleotide-disulfide reductase (PNDR), and the DNA oxidative damage-protective proteins DNA-binding ferritin-like protein (Dps), ribonucleotide reductase (NrdA) and nucleotide triphosphate (NTP) pyrophosphohydrolases (MutT1). Changes in polynucleotide phosphorylase (PNPase) plus enolase, which may play important roles in scavenging oxidatively damaged RNA, were also found. Following validation at the transcriptional level of differentially expressed proteins, the physiological and biochemical functions of BBMN68 Dps were further proven by in vitro and in vivo tests under oxidative stress. Our results reveal the key oxidative stress-protective proteins and DNA oxidative damage-protective proteins involved in the defence strategy of BBMN68 against oxygen, and provide the first proteomic information toward understanding the responses of Bifidobacterium and other anaerobes to oxygen stress.
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Xu
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Fanglei
authors.initials: F
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Fazheng
authors.initials: F
authors.lastname: Ren
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Pinglan
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, and Beijing Higher Institution Engineering Research Center of Animal Product, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Ma
mesh_terms: D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D006801:Humans; D018384:Oxidative Stress / Q000502:physiology*; D010100:Oxygen / Q000494:pharmacology; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D010100:Oxygen