title: Deinococcus reticulitermitis sp. nov., isolated from a termite gut.
abstract: Bacterial strain TM-1(T) was isolated from the gut of a wood-feeding termite, Reticulitermes chinensis Snyder. Cells of strain TM-1(T) were Gram-negative, spherical (1.0-2.0 µm in diameter), non-motile, non-sporulating and red-pigmented. Strain TM-1(T) was resistant to UV radiation, showing 34% survival after exposure to UV light at a dose of 100 J m(-2). Growth occurred at 20-40 °C (optimum, 30 °C), at pH 6.0-10.0 (optimum, pH 6.0-7.0), and in the presence of 0-1% (w/v) NaCl (optimum, 0-0.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TM-1(T) was related to members of the genus Deinococcus, with sequence similarities ranging from 87.0 to 94.0%. The peptidoglycan of strain TM-1(T) contained ornithine, alanine, glycine and glutamic acid. The most abundant cellular fatty acids of strain TM-1(T) were summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c; 22.3%) and C(16:0) (37.5%). MK-8 was the predominant quinone. The polar lipid profile contained three glycophospholipids, six glycolipids, one aminolipid and three unknown lipids. DNA of the type strain had a G+C content of 65.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain TM-1(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus reticulitermitis sp. nov. is proposed, with TM-1(T) (=CGMCC 1.10218(T)=NBRC 106334(T)) as the type strain.
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Huazhu
authors.initials: H
authors.lastname: Hong
authors.affiliation: Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Life Sciences, Central China Normal University, Wuhan 430079, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D020049:Isoptera / Q000382:microbiology*; D050296:Microbial Viability / Q000528:radiation effects; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride