title: Isolation and characterization of a fungus Aspergillus sp. strain F-3 capable of degrading alkali lignin.
abstract: A fungus strain F-3 was selected from fungal strains isolated from forest soil in Dalian of China. It was identified as one Aspergillus sp. stain F-3 with its morphologic, cultural characteristics and high homology to the genus of rDNA sequence. The budges or thickened node-like structures are peculiar structures of hyphae of the strain. The fungus degraded 65% of alkali lignin (2,000 mg l(-1)) after day 8 of incubation at 30°C at pH 7. The removal of colority was up to 100% at 8 days. The biodegradation of lignin by Aspergillus sp. F-3 favored initial pH 7.0. Excess acid or alkali conditions were not propitious to lignin decomposing. Addition of ammonium L: -tartrate or glucose delayed or repressed biodegradation activities. During lignin degradation, manganese peroxidase (28.2 U l(-1)) and laccase (3.5 U l(-1))activities were detected after day 7 of incubation. GC-MS analysis of biodegraded products showed strain F-3 could convert alkali lignin into small molecules or other utilizable products. Strain F-3 may co-culture with white rot fungus and decompose alkali lignin effectively.
authors.affiliation: School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, China. yangyusuo@dlu.edu.cn
authors.forename: Y S
authors.initials: YS
authors.lastname: Yang
authors.forename: J T
authors.initials: JT
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Lu
authors.forename: Y L
authors.initials: YL
authors.lastname: Yuan
authors.forename: L H
authors.initials: LH
authors.lastname: Zhao
mesh_terms: D001230:Aspergillus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics / Q000378:metabolism; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D005656:Fungal Proteins; D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase