title: Evaluation of scaffolds based on α-tricalcium phosphate cements for tissue engineering applications.
abstract: Growth of cells in 3-D porous scaffolds has gained importance in the field of tissue engineering. The scaffolds guide cellular growth, synthesize extracellular matrix and other biological molecules, and make the formation of tissues and functional organs easier. The aim of this study is to use α-tricalcium phosphate cement in order to obtain new types of scaffolds with the aid of paraffin spheres as pore generators. The porosity of the scaffolds produced with paraffin spheres was analyzed and compared to the literature, and the study of scaffold permeability using the Forchheimer equation allowed the analysis of pore interconnectivity. In vitro tests showed the behavior of scaffolds in solutions of simulated body fluid, and viability and cell proliferation were also evaluated. The results show the potential use of the materials developed for scaffolds for use in tissue engineering applications.
authors.affiliation: Departamento de Engenharia de Materiais, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. jefmac@gmail.com
authors.forename: Jéferson L de Moraes
authors.initials: JL
authors.lastname: Machado
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: Giehl
authors.forename: Nance B
authors.initials: NB
authors.lastname: Nardi
authors.forename: Luis A
authors.initials: LA
authors.lastname: dos Santos
mesh_terms: D000465:Algorithms; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; C018392:tricalcium phosphate