title: Development and validation of an analytical method for the determination of trans- and cis-resveratrol in wine: analysis of its contents in 186 Portuguese red wines.
abstract: A simple procedure based on solid-phase extraction and high performance liquid chromatography coupled to diode array detector has been developed and validated for the qualitative and quantitative analysis of cis- and trans-resveratrol in wines. The method was linear from 0.025 (lower limit of quantitation, LLOQ) to 15 μg/mL for trans-resveratrol and from 0.023 (LLOQ) to 0.92 μg/mL for cis-resveratrol, with correlation coefficients higher than 0.99 for both isomers. Intra- and interday precision and accuracy were in conformity with the criteria normally accepted in method validation, that is, CVs inferior to 15% and mean relative errors within a ±14% interval. The extraction presented mean efficiencies close to 100% for both analytes. The validated methodology was applied to 186 Portuguese red wines from different regions, grape varieties and vintage. The results obtained showed that the content of trans-resveratrol in red wines ranged from 0.05 to 10.9 μg/mL, while the concentrations of cis-resveratrol ranged from 0.04 to 8.71 μg/mL.
authors.affiliation: CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6201-556 Covilhã, Portugal.
authors.forename: Luísa
authors.initials: L
authors.lastname: Paulo
authors.forename: Fernanda
authors.initials: F
authors.lastname: Domingues
authors.forename: João António
authors.initials: JA
authors.lastname: Queiroz
authors.forename: Eugenia
authors.initials: E
authors.lastname: Gallardo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D011174:Portugal; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D013267:Stilbenes; D000077185:Resveratrol