title: [Evaluation of Chinese traditional patent medicines against influenza virus in vitro].
abstract: To study in vitro anti-influenza viral activities of Chinese traditional patent medicines for influenza prevention and treatment, neuraminidase (NA) activity assay was used to examine NA inhibitory activity of 33 Chinese traditional patent medicines through fluorimetric assay, and influenza virus induced cytopathic effect (CPE) inhibition assay was used to verify their anti-influenza viral activities in vitro. The assay results showed that most liquid preparations displayed relatively high NA inhibitory activities, such as Shuanghuanglian oral liquid, Qingkailing oral liquid, Qingre Jiedu oral liquid, and Reduning injection. Among liquid preparations, Shuanghuanglian oral liquid not only displayed the highest NA inhibitory effect, but also exhibited obvious in vitro anti-viral activity in CPE experiment. Among solid preparations, Shuanghuanglian powder for injection showed the highest activity on NA inhibition, and Fufang Yuxingcao tablet showed relatively strong anti-influenza viral activity in CPE cells. From the results, it can be concluded that most Chinese traditional patent medicines possessed NA inhibitory activity, but only a few of them displayed significant in vitro anti-influenza viral activities. These results will provide important information for the isolation of active constituents, and for the clinical uses of Chinese traditional patent medicines for influenza treatment and prevention.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Zu
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Ai-lin
authors.initials: AL
authors.lastname: Liu
authors.forename: Guan-hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000201:enzymology; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000201:enzymology; D009981:Influenza B virus / Q000187:drug effects / Q000201:enzymology; D008516:Medicine, Chinese Traditional; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; C504928:Qingkailing; C000596355:reduning; C516400:shuang-huang-lian; D009439:Neuraminidase