title: Two-photon probes for Zn2+ ions with various dissociation constants. Detection of Zn2+ ions in live cells and tissues by two-photon microscopy.
abstract: A series of Zn(2+)-selective two-photon fluorescent probes (AZnM1-AZnN) that had a wide range of dissociation constants (K(d) (TP) =8 nm-12 μM) were synthesized. These probes showed appreciable water solubility (>3 μM), cell permeability, high photostability, pH insensitivity at pH>7, significant two-photon action cross-sections (86-110 GM) upon complexation with Zn(2+), and can detect the Zn(2+) ions in HeLa cells and in living tissue slices of rat hippocampal at a depth of >80 μm without mistargeting and photobleaching problems. These probes can potentially find application in the detection of various amounts of Zn(2+) ions in live cells and intact tissues.
authors.affiliation: Department of Chemistry, Research Institute for Natural Sciences, Korea University, 1-Anamdong, Seoul 136-701, Korea.
authors.forename: Isravel Antony
authors.initials: IA
authors.lastname: Danish
authors.forename: Chang Su
authors.initials: CS
authors.lastname: Lim
authors.forename: Yu Shun
authors.initials: YS
authors.lastname: Tian
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Han
authors.forename: Min Young
authors.initials: MY
authors.lastname: Kang
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006624:Hippocampus / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D017785:Photons; D051381:Rats; D015032:Zinc / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D015032:Zinc