title: A genetic algorithm-based Boolean delay model of intracellular signal transduction in inflammation.
abstract: BACKGROUND
  Signal transduction is the major mechanism through which cells transmit external stimuli to evoke intracellular biochemical responses. Understanding relationship between external stimuli and corresponding cellular responses, as well as the subsequent effects on downstream genes, is a major challenge in systems biology. Thus, a systematic approach to integrate experimental data and qualitative knowledge to identify the physiological consequences of environmental stimuli is needed.
  
  
  RESULTS
  In present study, we employed a genetic algorithm-based Boolean model to represent NF-κB signaling pathway. We were able to capture feedback and crosstalk characteristics to enhance our understanding on the acute and chronic inflammatory response. Key network components affecting the response dynamics were identified.
  
  
  CONCLUSIONS
  We designed an effective algorithm to elucidate the process of immune response using comprehensive knowledge about network structure and limited experimental data on dynamic responses. This approach can potentially be implemented for large-scale analysis on cellular processes and organism behaviors.
authors.affiliation: Department of Computer Science, National Tsing Hua University, Hsinchu, 30013 Taiwan, ROC. g9662584@oz.nthu.edu.tw
authors.forename: Chu Chun
authors.initials: CC
authors.lastname: Kang
authors.forename: Yung Jen
authors.initials: YJ
authors.lastname: Chuang
authors.forename: Kai Che
authors.initials: KC
authors.lastname: Tung
authors.forename: Chun Cheih
authors.initials: CC
authors.lastname: Chao
authors.forename: Chuan Yi
authors.initials: CY
authors.lastname: Tang
authors.forename: Shih Chi
authors.initials: SC
authors.lastname: Peng
authors.forename: David Shan Hill
authors.initials: DS
authors.lastname: Wong
mesh_terms: D000465:Algorithms; D007249:Inflammation / Q000276:immunology / Q000378:metabolism*; D008954:Models, Biological; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism*; D020239:Receptor Cross-Talk; D015398:Signal Transduction
chemical_list: D016328:NF-kappa B