title: Cell Preparation and Multicolor FISH in 3D Preserved Cultured Mammalian Cells.
abstract: INTRODUCTIONFluorescence in situ hybridization (FISH) on three-dimensional preserved nuclei (3D-FISH) in combination with three-dimensional-microscopy and image reconstruction is an efficient tool to analyze the arrangement of distinct nuclear targets such as entire chromosome territories, chromosomal subregions, or single gene loci on a single-cell level. This protocol focuses on fixation, pretreatments, and 3D-FISH on cultured mammalian cells. It can be applied to a variety of cell types growing adherently or in suspension.
authors.affiliation: Ludwig-Maximilians University Munich, Department of Biology II, AG Thomas Cremer (Chair of Anthropology and Human Genetics), 82152 Martinsried-Planegg, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Cremer
authors.forename: Stefan
authors.initials: S
authors.lastname: Müller
authors.forename: Daniela
authors.initials: D
authors.lastname: Köhler
authors.forename: Alessandro
authors.initials: A
authors.lastname: Brero
authors.forename: Irina
authors.initials: I
authors.lastname: Solovei