title: Swept-source optical coherence tomography of lower limb wound healing with histopathological correlation.
abstract: Direct noninvasive visualization of wound bed with depth information is important to understand the tissue repair. We correlate skin swept-source-optical coherence tomography (OCT) with histopathological and immunohistochemical evaluation on traumatic lower limb wounds under honey dressing to compare and assess the tissue repair features acquired noninvasively and invasively. Analysis of optical biopsy identifies an uppermost brighter band for stratum corneum with region specific thickness (p < 0.0001) and gray-level intensity (p < 0.0001) variation. Below the stratum corneum, variation in optical intensities is remarkable in different regions of the wound bed. Correlation between OCT and microscopic observations are explored especially in respect to progressive growth and maturation of the epithelial and subepithelial components. Characteristic transition of uniform hypolucid band in OCT image for depigmented zone to wavy highly lucid band in the pigmented zone could be directly correlated with the microscopic findings. The transformation of prematured epithelium of depigmented area, with low expression of E-cadherin, to matured epithelium with higher E-cadherin expression in pigmented zone, implicated plausible change in their optical properties as depicted in OCT. This correlated evaluation of multimodal images demonstrates applicability of swept-source-OCT in wound research and importance of integrated approach in validation of new technology.
authors.affiliation: IIT Kharagpur, School of Medical Science and Technology, Kharagpur, West Bengal 721302 India. ananya.pariksha@gmail.com
authors.forename: Ananya
authors.initials: A
authors.lastname: Barui
authors.forename: Provas
authors.initials: P
authors.lastname: Banerjee
authors.forename: Rusha
authors.initials: R
authors.lastname: Patra
authors.forename: Raunak Kumar
authors.initials: RK
authors.lastname: Das
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
authors.forename: Pranab K
authors.initials: PK
authors.lastname: Dutta
authors.forename: Jyotirmoy
authors.initials: J
authors.lastname: Chatterjee
mesh_terms: D000293:Adolescent; D000328:Adult; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation*; D007869:Leg Injuries / Q000473:pathology*; D008029:Lighting / Q000295:instrumentation*; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D041623:Tomography, Optical Coherence / Q000295:instrumentation*; D014945:Wound Healing; D055815:Young Adult