title: [Icariin promotes osteogenic differentiation of rat bone marrow stromal cells in vitro].
abstract: OBJECTIVE
  To investigate the effects of icariin on the osteogenic differentiation of rat bone marrow stromal cells (rBMSCs).
  
  
  METHODS
  rBMSCs were cultured by adherence screening method. Icariin was supplemented into the culture at 1 x 10(-5) mol x L(-1). The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, CFU-F(ALP) and mineralized bone modulus were compared between the icariin-supplemented group and the control group. Total RNA was isolated and the gene expression of bFGF, IGF-1, Osterix(OSX) and Runx-2 was investigated by RT Real-time PCR.
  
  
  RESULTS
  Icariin significantly improved ALP activity, CFU-F(ALP) amounts and mineralized modulus. It also can enhance the mRNA level of bFGF, IGF-1, Osterix and Runx-2.
  
  
  CONCLUSIONS
  Icariin enhances the osteogenic differentiation of rBMSCs significantly, which suggested that icariin has the potentiality to be a new drug of anti-osteoporosis or fracture healing.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China. zhaiyk1984@163.com
authors.forename: Yuankun
authors.initials: Y
authors.lastname: Zhai
authors.forename: Baofeng
authors.initials: B
authors.lastname: Ge
authors.forename: Huiping
authors.initials: H
authors.lastname: Ma
authors.forename: Leiguo
authors.initials: L
authors.lastname: Ming
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Li
authors.forename: Guozheng
authors.initials: G
authors.lastname: Cheng
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Keming
authors.initials: K
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase; C056599:icariin