title: Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis.
abstract: The feasibility of using high-resolution melting analysis (HRMA) was examined for its rapid and simple detection of 5 clinically relevant Candida species (C. albicans, C. glabrata, C. kefyr, C. parapsilosis, and C. guilliermondii). HRMA was able to differentiate clinical Candida species and resulted in being sensitive, reproducible, and inexpensive.
authors.affiliation: Mycoses Unit, Department of Infectious, Parasitic and Immune-mediated Disease, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy. silvia.arancia@iss.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Arancia
authors.forename: Silvia
authors.initials: S
authors.lastname: Sandini
authors.forename: Flavia
authors.initials: F
authors.lastname: De Bernardis
authors.forename: Daniela
authors.initials: D
authors.lastname: Fortini
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics*; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D004271:DNA, Fungal / Q000737:chemistry*; D006801:Humans; D016533:Mycological Typing Techniques / Q000191:economics / Q000379:methods*; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004271:DNA, Fungal