title: [Expression of tumor matrix markers rebuild in laryngeal cancer depends on number of smoked cigarettes].
abstract: Head and neck cancers, especially laryngeal carcinoma, are still an essential therapeutic problem worldwide. The most common risk factors which participate in initiation of cancer progression are tobacco smoking and alcohol abuse. The fundamental factor which confines effective therapy is tumor advanced stage at the moment of diagnosis. For proper determination of cancer progression there still is a need to identify new markers which can describe cancer cells and are also related in invasiveness and tumor matrix rebuild. The studies were performed on tissue samples divided in two groups: from patient who smoked less and above 20 cigarettes a day. We observed the expression of studied antigens as follows: MMP-2 in 100%, TGF-beta1 in 86% and TGF-betaRI in 79% cases. In tumor stroma we observed higher expression of MMP-2, TGF-beta1 in patients with higher exposure to tobacco smoke. Moreover in patients who smoked above 20 cigarettes, we detected higher expression of MMP-2 in tumor stroma (86% vs. 12.5%), compared with expression in cancer cells (3% vs. 3.25%). Expression of TGF-betaRI, in group I, was respectively higher in tumor stroma (16.8%) compared with cancer cells (6%). However we've found no expression of TGF-BRI in samples from patients smoking more then 20 cigarettes a day. In summary we may suggest, that the level of exposure on tobacco smoke play important role on changes in tumor markers expression.
authors.affiliation: Katedra i Zakład Patomorfologii Klinicznej, Uniwersytet Mikołaja Kopernika, Toruń, Collegium Medicum im. L. Rydygiera, Bydgoszcz.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bodnar
authors.forename: Paweł
authors.initials: P
authors.lastname: Burduk
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kaźmierczak
authors.forename: Andrzej
authors.initials: A
authors.lastname: Marszałek
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D018450:Disease Progression; D004781:Environmental Exposure / Q000032:analysis; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000737:chemistry*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis; D012907:Smoking / Q000453:epidemiology*; D014028:Tobacco Smoke Pollution / Q000032:analysis*; D053773:Transforming Growth Factor beta1 / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D018125:Receptors, Transforming Growth Factor beta; D014028:Tobacco Smoke Pollution; D053773:Transforming Growth Factor beta1; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D020778:Matrix Metalloproteinase 2