title: Supercritical angle fluorescence immunoassay platform.
abstract: An inexpensive and easy-to-use immunoassay platform for the sensitive detection of analytes is presented. It comprises single-use polymer test tubes and a compact fluorescence reader. The optics for the capture of supercritical angle fluorescence (SAF) has been built into the tubes allowing for the extremely sensitive readout of solid phase immunoassays in real time and without washing steps. One-step sandwich immunoassays with interleukin 2 (IL-2) were carried out with capture antibodies immobilized in the tubes. At a turn around time of 12 min, the limit of detection for IL-2 was 0.27 pM (4.5 pg/mL) and the linear range covered 3 orders of magnitude. The developed technology is also adaptable to well plates and has great potential of replacing the work-intensive and time-consuming enzyme-linked immunosorbant assay (ELISA).
authors.affiliation: Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ruckstuhl
authors.forename: Christian M
authors.initials: CM
authors.lastname: Winterflood
authors.forename: Stefan
authors.initials: S
authors.lastname: Seeger
mesh_terms: D007118:Immunoassay / Q000295:instrumentation*; D007376:Interleukin-2 / Q000032:analysis; D055620:Optical Phenomena; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D007376:Interleukin-2; D011108:Polymers