title: Rapid screening of lignans from Phyllanthus myrtifolius and stilbenoids from Syagrus romanzoffiana by HPLC-SPE-NMR.
abstract: BACKGROUND
  Application of on-line solid-phase extraction (SPE) as an interface between HPLC and NMR has gained great improvement in solving sensitivity problems and signal interferences by the eluents.
  
  
  OBJECTIVE
  Rapid analysis and characterisation by HPLC-SPE-NMR and LC/MS of the arylnaphthalene-type lignans present in Phyllanthus myrtifolius and the minor stilbenoids present in the polyphenol-rich fraction from the ethanol extract of the seeds of Syagrus romanzoffiana.
  
  
  METHODS
  Pretreatment of fractions by liquid-liquid partitioning, followed by Sephadex LH-20 fractionation, was found very useful to facilitate the focusing and analysis of the polyphenolic fraction. HPLC-DAD-SPE-NMR (400  MHz and 600 MHz) analysis was carried out using an Agilent 1100 liquid chromatography, followed by a Prospekt 2 automated solid-phase extraction unit, containing 96 HySphere-Resin GP cartridges (10 × 2 mm, 10-12 µm), which was connected to a 120 or 60 µL LC probe.
  
  
  RESULTS
  Seven arylnaphthalene-type lignans from the chloroform-soluble fraction of P. myrtifolius and nine stilbenoids from a polyphenol-rich butanol-soluble fraction of the seeds of S. romanzoffiana were characterised.
  
  
  CONCLUSIONS
  HPLC-SPE-NMR associated with HR-ESI/MS, which consumed only analytical amounts of partially purified mixtures, was demonstrated to be a good tool for rapid screening of both known and new natural products.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, 1 Jen-Ai Rd., Sec. 1, Taipei 10051, Taiwan, Republic of China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Sio-Hong
authors.initials: SH
authors.lastname: Lam
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Tseng
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
mesh_terms: D028023:Arecaceae / Q000737:chemistry*; D001572:Benzofurans / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D017705:Lignans / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D059808:Polyphenols; D012639:Seeds / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D013267:Stilbenes / Q000032:analysis / Q000737:chemistry; D013624:Taiwan
chemical_list: D001572:Benzofurans; D005419:Flavonoids; D017705:Lignans; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013267:Stilbenes; C550387:scirpusin A; C530523:scirpusin C