title: Effect of low-magnitude, high-frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells.
abstract: Whole body vibration (WBV), consisting of a low-magnitude, high-frequency (LMHF) signal, is anabolic to bone in vivo and may act through alteration of the lineage commitment of mesenchymal stromal cells (MSC). We investigated the effect of LMHF vibration on rat bone marrow-derived MSCs (rMSCs) in an in vitro system. We subjected rMSCs to repeated (six) bouts of 1-h vibration at 0.3g and 60 Hz in the presence of osteogenic (OS) induction medium. The OS differentiation of rMSCs under the loaded and non-loaded conditions was assessed by examining cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of various osteoblast-associated markers [ALP, Runx2, osterix (Osx), collagen type I alpha 1 (COL1A1), bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN)], and matrix mineralization. LMHF vibration did not enhance the OS differentiation of rMSCs. Surprisingly, the mRNA level of Osx, a transcription factor necessary for osteoblast formation, was decreased, and matrix mineralization was inhibited. Our findings suggest that LMHF vibration may exert its anabolic effects in vivo via mechanosensing of a cell type different from MSCs.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Esther
authors.initials: E
authors.lastname: Lau
authors.forename: W David
authors.initials: WD
authors.lastname: Lee
authors.forename: Jason
authors.initials: J
authors.lastname: Li
authors.forename: Andrew
authors.initials: A
authors.lastname: Xiao
authors.forename: John E
authors.initials: JE
authors.lastname: Davies
authors.forename: Qianhong
authors.initials: Q
authors.lastname: Wu
authors.forename: Liyun
authors.initials: L
authors.lastname: Wang
authors.forename: Lidan
authors.initials: L
authors.lastname: You
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D066298:In Vitro Techniques; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010011:Osteocytes / Q000166:cytology* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics; D014732:Vibration
chemical_list: D012333:RNA, Messenger; C495181:Sp7 protein, rat; D014157:Transcription Factors; D000469:Alkaline Phosphatase