title: [Effects of dihydroxy-stilbene compound Vam3 on airway inflammation, expression of ICAM-1, activities of NF-kappaB and MMP-9 in asthmatic mice].
abstract: The aim of the present study is to investigate the effects of Vam3 which is one of the dihydroxystilbene compounds on expressions of ICAM-1 in the lungs of OVA-induced asthmatic mice and the mechanisms of anti-airway inflammation. Balb/c mice were challenged with OVA inhalation. Lung tissues were stained with Mayer's hematoxylin and eosin for histopathologic examination. The expression of ICAM-1 in the lungs of mice was analyzed by Western blotting and immunohistochemistry method. The NF-kappaB activities were detected by NF-kappaB-luc reporter genetic transient transfection method. The activities of MMP-9 induced by LPS, TNF-alpha and PMA in THP-1 cells were determined by gelatin zymography method. The results showed that Vam3 could inhibit the expression of ICAM-1 in the OVA-induced mouse model. In addition, Vam3 could significantly suppress the activities of NF-kappaB in A549 cells and MMP-9 in THP-1 cells induced by LPS, TNF-alpha and PMA. These results suggested that Vam3 could alleviate the asthmatic inflammation by decreasing ICAM-1 expression in asthmatic mice, down regulating NF-kappaB and MMP-9 activities. Compound Vam3 showed inhibitory effects on inflammatory signal pathways involved in asthma.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Chun-suo
authors.initials: CS
authors.lastname: Yao
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Wu
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Xuan
authors.forename: Jin-ye
authors.initials: JY
authors.lastname: Bai
authors.forename: Gui-fang
authors.initials: GF
authors.lastname: Cheng
authors.forename: Mao
authors.initials: M
authors.lastname: Lin
authors.forename: Ming-chun
authors.initials: MC
authors.lastname: Wen
authors.forename: Qi
authors.initials: Q
authors.lastname: Hou
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism*; D001572:Benzofurans / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D010047:Ovalbumin; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D016328:NF-kappa B; D013267:Stilbenes; C521077:amurensin H; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse