title: Serum protein profiling of systemic lupus erythematosus and systemic sclerosis using recombinant antibody microarrays.
abstract: Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are two severe autoimmune connective tissue diseases. The fundamental knowledge about their etiology is limited and the conditions display complex pathogenesis, multifaceted presentations, and unpredictable courses. Despite significant efforts, the lack of fully validated biomarkers enabling diagnosis, classification, and monitoring of disease activity represents significant unmet clinical needs. In this discovery study, we have for the first time used recombinant antibody microarrays for miniaturized, multiplexed serum protein profiling of SLE and SSc, targeting mainly immunoregulatory proteins. The data showed that several candidate SLE-associated multiplexed serum biomarker signatures were delineated, reflecting disease (diagnosis), disease severity (phenotypic subsets), and disease activity. Selected differentially expressed markers were validated using orthogonal assays and a second, independent patient cohort. Further, biomarker signatures differentiating SLE versus SSc were demonstrated, and the observed differences increased with severity of SLE. In contrast, the data showed that the serum profiles of SSc versus healthy controls were more similar. Hence, we have shown that affinity proteomics could be used to de-convolute crude, nonfractionated serum proteomes, extracting molecular portraits of SLE and SSc, further enhancing our fundamental understanding of these complex autoimmune conditions.
authors.affiliation: Department of Immunotechnology, BMC D13, Lund University, SE-221 84 Lund, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Carlsson
authors.forename: Dirk M
authors.initials: DM
authors.lastname: Wuttge
authors.forename: Johan
authors.initials: J
authors.lastname: Ingvarsson
authors.forename: Anders A
authors.initials: AA
authors.lastname: Bengtsson
authors.forename: Gunnar
authors.initials: G
authors.lastname: Sturfelt
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D019151:Peptide Library; D010641:Phenotype; D011379:Prognosis; D040081:Protein Array Analysis; D020543:Proteome / Q000737:chemistry*; D012372:ROC Curve; D012595:Scleroderma, Systemic / Q000097:blood* / Q000175:diagnosis; D057127:Single-Chain Antibodies / Q000737:chemistry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D019151:Peptide Library; D020543:Proteome; D057127:Single-Chain Antibodies