title: [Establishment of embryonic stem cell for evaluation of development neurotoxicity and test the validation of embryonic stem cell].
abstract: OBJECTIVE
  To establish embryonic stem cell (EST) for evaluation of developmental neurontoxicity and test the validation of EST.
  
  
  METHODS
  RT-PCR method was used to study the influence of fluorouracil (5-FU), diphenylhydantoin (DPH) and Penicillin G at the different concentrations on neuron cells with nestin expression that differentiate from embryonic stem cells (ES). With the results of cells viability (ES and BALB/c) assessed by MTT, the developmental neurotoxicty characteristics of 5-FU, DPH and Penicillin G may be identified clearly. RESULTS; The ID50 D3 nestin concentration of three embryotoxicants were 0.017, 49.4 and 1139 microg/ml. Penicillin G, DPH and 5-FU were discriminated as none-, weak- and strong- developmental neurotoxicity. CONCLUSION; Test compounds among three classes were discriminated correctly. The EST on developmental neurontoxicity could be applied to the safety evaluation in vitro.
authors.affiliation: Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yu
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
mesh_terms: D000826:Animal Testing Alternatives / Q000379:methods*; D000818:Animals; D041702:BALB 3T3 Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects*; D005472:Fluorouracil / Q000633:toxicity; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010400:Penicillin G / Q000633:toxicity; D010672:Phenytoin / Q000633:toxicity; D015203:Reproducibility of Results; D018675:Toxicity Tests / Q000379:methods*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D014212:Tretinoin; D010672:Phenytoin; D010400:Penicillin G; D005472:Fluorouracil