title: Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy.
abstract: Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfil predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3-4Å resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.
authors.affiliation: Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02154, USA.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Grigorieff
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Harrison
mesh_terms: D003198:Computer Simulation; D020285:Cryoelectron Microscopy; D007091:Image Processing, Computer-Assisted; D008958:Models, Molecular; D019065:Virus Assembly; D014780:Viruses / Q000737:chemistry*