title: [Effects of shoutai pills on expression of Th1/Th2 cytokine in maternal-fetal interface and pregnancy outcome].
abstract: OBJECTIVE
  To evaluate its mechanism of inducing the maternal-fetal immune tolerance by studying the effects of Shoutai pills on the expression of Th1/Th2 cytokine and pregnancy in maternal-fetal interface of mice with recurrent spontaneous abortion (RSA).
  
  
  METHODS
  The normal pregnancy and RSA model were respectively induced with CBA/J x BALB/c and CBA/J x DBA/2. The mice with RSA were randomly divided into model group and low, middle and high dose groups of Shoutai pills. The mice were killed in 14 days after administration and embryo resorption rate was counted and their decidual and placental tissues were co-cultured to detect the expressions of IL-4, IL-10, IFN-gamma and TNF-alpha with ELISA.
  
  
  RESULTS
  The embryo resorption rate of the model group was significantly higher than the normal pregnancy, middle and high dose groups of Shoutai pills could decreased the embryo resorption rate of the mice with RSA (P < 0.05). All the doses in 3 groups of Shoutai pills could decreased the expression of IFN-gamma and TNF-alpha (P < 0. 05) and there was no obvious difference between normal pregnancy group and all groups of Shoutai pills. Middle and high doses of Shoutai pills could increased the expression of IL-4 and IL-10 (P < 0.05) and there was no obvious differences between normal pregnancy and high dose group of Shoutai pills.
  
  
  CONCLUSIONS
  The mechanism about Shoutai pills can change Th1 /Th2 cytokine towards Th2 bias, which induced the maternal-fetal immune tolerance.
authors.affiliation: First Affiliated Hospital of Guangzhou Medicine college, Guangzhou 510120, China. lmhlchy@126.com
authors.forename: Maohua
authors.initials: M
authors.lastname: Lai
authors.forename: Zhaoling
authors.initials: Z
authors.lastname: You
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.forename: Fangguo
authors.initials: F
authors.lastname: Lu
authors.forename: Dongmei
authors.initials: D
authors.lastname: He
authors.forename: Huiping
authors.initials: H
authors.lastname: Liu
authors.forename: Sheng
authors.initials: S
authors.lastname: Yin
mesh_terms: D000026:Abortion, Habitual / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D016207:Cytokines / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D020964:Embryo Loss; D005260:Female; D006801:Humans; D008297:Male; D008431:Maternal-Fetal Exchange / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011247:Pregnancy; D011256:Pregnancy Outcome; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal