title: Neuromedin S regulates cardiovascular function through the sympathetic nervous system in mice.
abstract: Intracerebroventricular (icv) injection of neuromedin S (NMS) in mice increased the heart rate in a dose-dependent manner. On the other hand, genetically NMS deficient mice (NMS-KO mice) exhibited a decreased heart rate and significant extension of the QRS and PR interval in the electrocardiogram complex. Although treatment with a parasympathetic nerve blocker, methylscopolamine, and a sympathetic nerve blocker, timolol, respectively increased and decreased the heart rate in both NMS-KO and wild-type mice, the extent of the decrease induced by timolol was smaller in NMS-KO than in wild-type mice. In addition, pretreatment with timolol completely inhibited the NMS-induced heart rate increase in wild-type mice. No expression of mRNA for NMS or the NMS receptor was evident in the heart by RT-PCR analysis. These results suggest that endogenous NMS may regulate cardiovascular function by activating the sympathetic nervous system.
authors.affiliation: Department of Veterinary Physiology, Faculty of Agriculture, Miyazaki University, Miyazaki 889-2192, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Nakahara
authors.forename: Keisuke
authors.initials: K
authors.lastname: Maruyama
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Katayama
authors.forename: Kenji
authors.initials: K
authors.lastname: Mori
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Noboru
authors.initials: N
authors.lastname: Murakami
mesh_terms: D000818:Animals; D001340:Autonomic Nerve Block; D001794:Blood Pressure / Q000235:genetics / Q000502:physiology*; D004562:Electrocardiography; D006339:Heart Rate / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009479:Neuropeptides; C497950:neuromedin S