title: Role of B regulatory subunits of protein phosphatase type 2A in myosin II assembly control in Dictyostelium discoideum.
abstract: In Dictyostelium discoideum, myosin II resides predominantly in a soluble pool as the result of phosphorylation of the myosin heavy chain (MHC), and dephosphorylation of the MHC is required for myosin II filament assembly, recruitment to the cytoskeleton, and force production. Protein phosphatase type 2A (PP2A) was identified in earlier studies in Dictyostelium as a key biochemical activity that can drive MHC dephosphorylation. We report here gene targeting and cell biological studies addressing the roles of candidate PP2A B regulatory subunits (phr2aBα and phr2aBβ) in myosin II assembly control in vivo. Dictyostelium phr2aBα- and phr2aBβ-null cells show delayed development, reduction in the assembly of myosin II in cytoskeletal ghost assays, and defects in cytokinesis when grown in suspension compared to parental cell lines. These results demonstrate that the PP2A B subunits phr2aBα and phr2aBβ contribute to myosin II assembly control in vivo, with phr2aBα having the predominant role facilitating MHC dephosphorylation to facilitate filament assembly.
authors.affiliation: Department of Cell Biology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
authors.forename: Vandana
authors.initials: V
authors.lastname: Rai
authors.forename: Thomas T
authors.initials: TT
authors.lastname: Egelhoff
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D048749:Cytokinesis / Q000502:physiology; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D054648:Protein Phosphatase 2 / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D015800:Protozoan Proteins; D054648:Protein Phosphatase 2; D024462:Myosin Type II