title: Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures.
abstract: Information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV), of the Arenaviridae, Filoviridae, Bunyaviridae, and Flavivridae virus families by performing quantitative analysis of cell culture supernatants by (i) electron microscopy for the quantification of virus particles, (ii) quantitative real time PCR for the quantification of genomes, and (iii) determination of focus forming units by coating fluorescent antibodies to infected cell monolayers for the quantification of virus infectivity.The comparative analysis revealed that filovirus and RVFV replication results in a surplus of genomes but varying degrees of packaging efficiency and infectious particles. More efficient replication and packaging was observed for Lassa virus, and Dengue virus resulting in a better yield of infectious particles while, YFV turned out to be most efficient with only 4 particles inducing one FFU. For Crimean-Congo haemorrhagic fever virus (CCHFV) a surplus of empty shells was observed with only one in 24 particles equipped with a genome. The complete particles turned out to be extraordinarily infectious.
authors.affiliation: Department of Virology, University Medical Center Göttingen, Germany. mweidma@gwdg.de
authors.forename: Manfred
authors.initials: M
authors.lastname: Weidmann
authors.forename: Amadou A
authors.initials: AA
authors.lastname: Sall
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Manuguerra
authors.forename: Lamine
authors.initials: L
authors.lastname: Koivogui
authors.forename: Aime
authors.initials: A
authors.lastname: Adjami
authors.forename: Faye Fatou
authors.initials: FF
authors.lastname: Traoré
authors.forename: Kjell-Olof
authors.initials: KO
authors.lastname: Hedlund
authors.forename: Gunnel
authors.initials: G
authors.lastname: Lindegren
authors.forename: Ali
authors.initials: A
authors.lastname: Mirazimi
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D016679:Genome, Viral; D006482:Hemorrhagic Fevers, Viral / Q000821:virology*; D006801:Humans; D012328:RNA Viruses / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D014771:Virion / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D014776:Virus Cultivation