title: Nanoparticle-mediated intracellular lipid accumulation during C2C12 cell differentiation.
abstract: In this report, we sought to elucidate whether multiwall carbon nanotubes are involved in the modulation of the proliferation and differentiation of the skeletal muscle cell line C2C12. Skeletal muscle is a major mass peripheral tissue that accounts for 40% of total body weight and 50% of energy consumption. We focused on the differentiation pathway of myoblasts after exposure to a vapor-grown carbon fiber, HTT2800, which is one of the most highly purified carbon nanotubes. This treatment leads in parallel to the expression of a typical adipose differentiation program. We found that HTT2800 stimulated intracellular lipid accumulation in C2C12 cells. We have also shown by quantified PCR analysis that the expression of adipose-related genes was markedly upregulated during HTT2800 exposure. Taken together, these results suggest that HTT2800 specifically converts the differentiation pathway of C2C12 myoblasts to that of adipoblast-like cells.
authors.affiliation: Department of Integrative Physiology and Bio-System Control, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. ttamotsu@shinshu-u.ac.jp
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Hisao
authors.initials: H
authors.lastname: Haniu
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D015870:Gene Expression; D050356:Lipid Metabolism; D051379:Mice; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D037742:Nanotubes, Carbon
chemical_list: D037742:Nanotubes, Carbon