title: Single-subject statistical mapping of acute brain hypoxia in the rat following middle cerebral artery occlusion: a microPET study.
abstract: No study so far has attempted to map the 3D topography of brain hypoxia in the individual rat in vivo following middle cerebral artery occlusion (MCAo). In a previous microPET study, we reported that (18)F-fluoromisonidazole ((18)F-MISO) trapping in the brain after MCAo was specific for the hypoxic viable tissue. Here, we used (18)F-MISO microPET to map the 3D topography of brain hypoxia in the acute stage of permanent distal MCAo in individual spontaneously hypertensive rats. Normal rats were also studied. (18)F-MISO was intravenously injected approximately 1 h after clip placement and PET data were acquired for 2 hours. Animals were sacrificed and the brains harvested 48 h later for infarct mapping using standard histopathology. As expected, continuous (18)F-MISO trapping was found over the affected relative to unaffected and control MCA cortex. Using single-subject voxel-based statistical mapping, tracer accumulation 90-120 min after injection was consistently significantly higher in the anterior MCA cortex (proximal relative to clip site) and gradually decreased towards posterior areas, a pattern consistent with the classic penumbra concept. The data also suggested that (i) a portion of the significant (18)F-MISO trapping area may sit outside the contours of the final infarct despite the permanent MCAo, suggesting that (18)F-MISO may be a marker not only of severe (penumbral) but also of milder (oligemic) hypoxia, and (ii) small portions of the final infarct may not exhibit early tracer trapping, suggesting that by the time the tracer was administered this tissue had already progressed to irreversible damage. This study shows the feasibility of single-subject mapping of brain hypoxia following MCAo in the rat, which has potential applications in pathophysiological investigations.
authors.affiliation: Department of Clinical Neurosciences, Stroke Research Group, University of Cambridge, Cambridge, UK.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takasawa
authors.forename: John S
authors.initials: JS
authors.lastname: Beech
authors.forename: Tim D
authors.initials: TD
authors.lastname: Fryer
authors.forename: P Simon
authors.initials: PS
authors.lastname: Jones
authors.forename: Tahir
authors.initials: T
authors.lastname: Ahmed
authors.forename: Rob
authors.initials: R
authors.lastname: Smith
authors.forename: Franklin I
authors.initials: FI
authors.lastname: Aigbirhio
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Baron
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000473:pathology; D001931:Brain Mapping / Q000379:methods*; D004195:Disease Models, Animal; D002534:Hypoxia, Brain / Q000000981:diagnostic imaging* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D020244:Infarction, Middle Cerebral Artery / Q000000981:diagnostic imaging* / Q000473:pathology; D020768:Middle Cerebral Artery / Q000000981:diagnostic imaging* / Q000473:pathology; D011877:Radionuclide Imaging; D051381:Rats; D013997:Time Factors