title: [Analyses of serological and genetic characteristics on novel H1N1 influenza A virus from the infected patient in Shenzhen].
abstract: Analysis of serological and genetic characteristics on 2009 swine-origin influenza A (H1N1) virus (S-OIV) isolated from four patients with severe disease in Shenzhen were performed. Microneutralization assay showed that the neutralizing antibody titers of the infected patients did not exceed 1 : 20 in a short term post infection, which could not neutralize the viruses efficiently. Hemagglutination inhibition (HI) tests confirmed that the antigenicity of S-OIV from the patients was distinct from the seasonal influenza A virus, but similar to the reference strains of S-OIV. Phylogenetic and molecular analysis showed that S-OIV from the patients still belonged to the classical swine lineages and did not have the genetic characteristics of highly pathogenic influenza virus. Several amino acid residue mutations on HA protein were detected, which seemed not to affect the virulence and pathogenicity of the viruses. Further, A His 275 Tyr mutation on NA protein of a virus strain was detected, which induced the oseltamivir resistance of the virus.
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. hhwcl@163.com
authors.forename: Chun-Li
authors.initials: CL
authors.lastname: Wu
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Cheng
authors.forename: Xing
authors.initials: X
authors.lastname: Lv
authors.forename: Jian-Fan
authors.initials: JF
authors.lastname: He
authors.forename: Yun-Mei
authors.initials: YM
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Shi-Song
authors.initials: SS
authors.lastname: Fang
authors.forename: Ren-Li
authors.initials: RL
authors.lastname: Zhang
authors.forename: Jin-Quan
authors.initials: JQ
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014764:Viral Proteins