title: [Polysorbate-80 modified neurotoxin nanoparticle with its transport and cytotoxicity against blood-brain barrier].
abstract: This study was aimed at the transport across blood-brain barrier (BBB) of polysorbate-80 modified neurotoxin loaded polybutylcyanoacrylate nanoparticle (P-80-NT-NP) and its cytotoxicity. An in vitro model of BBB using rat brain microvascular endothelial cells (rBMECs) was established. The cytotoxicity of P-80-NT-NP was measured by the MTT assays, where neurotoxin (NT), nanoparticle (NP), neurotoxin nanoparticle (NT-NP) as control, and the permeability of P-80-NT-NP was determined by using of Millicell insert coculture with rBMECs and fluorescence spectrophotometry. MTT results showed that NT, NP, NT-NP and P-80-NT-NP were avirulent to rBMECs when the concentration of NT was lower than 200 ng x mL(-1). But the cytotoxicity of NP, NT-NP and P-80-NT-NP would be augmented accordingly as concentration increased (P < 0.01), causing obvious reductions of cell survival rate, with no significant difference between them (P > 0.05). When the concentration of NT was 150 ng x mL(-1), the permeability on rBMECs of P-80-NT-NP and NT-NP were both significantly higher than that of NT (P < 0.01), and the permeability of P-80-NT-NP was greater than that of NT-NP (P < 0.05). In conclusion, polysorbate-80 modified neurotoxin nanoparticles can transport across the BBB, while concentration of NT is greater than 200 ng x mL(-1), P-80-NT-NP has a little cytotoxicity against rBMECs.
authors.affiliation: College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Yan-min
authors.initials: YM
authors.lastname: Zhao
authors.forename: Ai-xiao
authors.initials: AX
authors.lastname: Xia
authors.forename: Ying-hui
authors.initials: YH
authors.lastname: Wei
authors.forename: Ye-ping
authors.initials: YP
authors.lastname: Ruan
authors.forename: Fan-zhu
authors.initials: FZ
authors.lastname: Li
mesh_terms: D000818:Animals; D001692:Biological Transport; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply; D002199:Capillary Permeability; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004337:Drug Carriers; D017097:Electric Impedance; D004659:Enbucrilate / Q000737:chemistry / Q000633:toxicity; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D008297:Male; D053758:Nanoparticles; D009498:Neurotoxins / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004337:Drug Carriers; D009498:Neurotoxins; D011136:Polysorbates; D004659:Enbucrilate