title: In vitro biocompatibility and mechanical performance of titanium doped high calcium oxide metaphosphate-based glasses.
abstract: This study challenged to produce phosphate-based glasses (PBG) for the treatment of osseous defects. The glasses contained, among other components, 40 mol% CaO and 1-5 mol% TiO(2). The mechanical performance and in vitro biocompatibility using both human osteosarcoma and primary osteoblasts were carried out. Incorporation of TiO(2) into PBG had no significant effect on strength and modulus. These glasses encouraged attachment and maintained high viability of osteosarcoma cells similar to the positive control surface. Cells grown directly (on glasses) or indirectly (in the presence of glass extracts) showed similar proliferation pattern to the positive control cells with no significant effect of TiO(2) detected. Increasing TiO(2) content, however, has a profound effect on cytoskeleton organization and spreading and maturation of primary osteoblasts. It is believed that TiO(2) might have acted as a chemical cue-modulating cells response, and hence the substrates supported maturation/mineralization of the primary osteoblasts.
authors.affiliation: Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: Ensanya A
authors.initials: EA
authors.lastname: Abou Neel
authors.forename: Wojciech
authors.initials: W
authors.lastname: Chrzanowski
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Knowles