title: Selective utilization of Toll-like receptor and MyD88 signaling in B cells for enhancement of the antiviral germinal center response.
abstract: The contribution of Toll-like receptor (TLR) signaling to T cell-dependent (TD) antibody responses was assessed by using mice lacking the TLR signaling adaptor MyD88 in individual cell types. When a soluble TLR9 ligand was used as adjuvant for a protein antigen, MyD88 was required in dendritic cells but not in B cells to enhance the TD antibody response, regardless of the inherent immunogenicity of the antigen. In contrast, a TLR9 ligand contained within a virus-like particle substantially augmented the TD germinal center IgG antibody response, and this augmentation required B cell MyD88. The ability of B cells to discriminate between antigens based on the physical form of a TLR ligand probably reflects an adaptation to facilitate strong antiviral antibody responses.
authors.affiliation: Department of Microbiology & Immunology, University of California, San Francisco, CA 94143, USA. baidong_hou@ibp.ac.cn
authors.forename: Baidong
authors.initials: B
authors.lastname: Hou
authors.forename: Philippe
authors.initials: P
authors.lastname: Saudan
authors.forename: Gary
authors.initials: G
authors.lastname: Ott
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Wheeler
authors.forename: Ming
authors.initials: M
authors.lastname: Ji
authors.forename: Lili
authors.initials: L
authors.lastname: Kuzmich
authors.forename: Linda M
authors.initials: LM
authors.lastname: Lee
authors.forename: Robert L
authors.initials: RL
authors.lastname: Coffman
authors.forename: Martin F
authors.initials: MF
authors.lastname: Bachmann
authors.forename: Anthony L
authors.initials: AL
authors.lastname: DeFranco
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D018858:Germinal Center / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018448:Models, Immunological; D053594:Myeloid Differentiation Factor 88 / Q000276:immunology*; D009975:Orthomyxoviridae / Q000276:immunology; D015398:Signal Transduction; D051217:Toll-Like Receptor 9 / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D053594:Myeloid Differentiation Factor 88; D051217:Toll-Like Receptor 9