title: Peroxyl-induced oxidative stress in aging erythrocytes of rat.
abstract: This study aims at determining the possible changes in intracellular calcium (Ca (i) (2+) ), plasma membrane calcium ATPase (PMCA) activity and phosphatidylserine (PS) along with glutathione (GSH) level in response to an oxidant challenge in vitro. Erythrocytes were isolated on Percoll and incubated with 2, 2'azobis (2-aminopropane) hydrochloride (AAPH) as well as with vitamin C preceding AAPH incubation. Membrane integrity in terms of hemolysis was negatively related to acetylcholine esterase (AChE) activity with the extent of reduction under OS being higher in the old erythrocyte than in the young. A divergent pattern was seen towards lower PMCA and higher (Ca (i) (2+) ) in the young and old cells. However, the PMCA activity in the stressed young cell was high when pre-treated with vitamin C. PS externalization in the young under OS is perhaps analogous to normal aging, with vitamin C preventing premature death. These findings suggest that young erythrocytes may benefit from vitamin C in therapies addressed towards the mechanisms underlying the reduced effects of OS.
authors.affiliation: Department of Zoology, Laboratory of Gerontology, Bangalore University, Bangalore, 560 056, India. asuba@blr.vsnl.net.in
authors.forename: S
authors.initials: S
authors.lastname: Asha Devi
authors.forename: C S
authors.initials: CS
authors.lastname: Shiva Shankar Reddy
authors.forename: M V V
authors.initials: MV
authors.lastname: Subramanyam
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood; D002118:Calcium / Q000097:blood; D004905:Erythrocyte Aging / Q000187:drug effects* / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D066298:In Vitro Techniques; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010545:Peroxides / Q000633:toxicity*; D010718:Phosphatidylserines / Q000097:blood; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010545:Peroxides; D010718:Phosphatidylserines; C049375:perhydroxyl radical; D000110:Acetylcholinesterase; D053499:Plasma Membrane Calcium-Transporting ATPases; D005978:Glutathione; D001205:Ascorbic Acid; D002118:Calcium