title: Oligoethylenimines grafted to PEGylated poly(β-amino ester)s for gene delivery.
abstract: Novel polymers composed of net-like PEGylated poly(β-amino ester) (N-P-1, M(w) = 6900 or N-P-2, M(w) = 21,400) and oligoethylenimine (OEI) (OEI423 or OEI600) were synthesized and evaluated as gene carriers. The molecular weights of these polymers were well-controlled by the concentration of the cross-linking reaction. The synthesized polymers showed high biodegradability, less cytotoxicity, and efficient DNA retard ability. The N-P-1-OEI600/DNA complex showed much slower aggregation in the presence of 10 and 20% serum solutions. In vitro transfection assays, N-P-2-OEI423, N-P-1-OEI600, and N-P-2-OEI600 showed enhanced transfection efficiency compared with the PEI25K control in the presence or in the absence of serum in different cell lines. In particular, in Cos-7 cells, the transfection efficiency of N-P-1-OEI600 was 20.9 times higher than that of PEI25K in the presence of serum. The polymer/DNA complex stability, lower cytotoxicity, and higher transfection efficiency in the presence of serum revealed that N-P-1-OEI600 could be a potential nonviral gene carrier for In Vivo application.
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Xia
authors.forename: Huayu
authors.initials: H
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Zhaopei
authors.initials: Z
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D004952:Esters; D018014:Gene Transfer Techniques; D018613:Microscopy, Confocal; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004952:Esters; D011092:Polyethylene Glycols; D011094:Polyethyleneimine