title: Synthesis of a trimeric gp120 epitope mimic conjugated to a T-helper peptide to improve antigenicity.
abstract: A fully synthetic trivalent mimotope of gp120 conjugated to pan allelic HLA DR binding epitope was prepared using solid-phase peptide synthesis and optimized copper-catalyzed azide-alkyne cycloaddition. The methodology efficiently provides chemically uniform heteromultimeric peptide constructs with enhanced binding, avidity, and specificity toward an established HIV-neutralizing human antibody, MAb b12. The versatile synthetic strategy serves as a powerful platform for the development of synthetic peptides as potential HIV-1 vaccine candidates.
authors.affiliation: Chemistry Department, Campus Mass Spectrometry Facility, University of California at Davis, 1 Shields Avenue, Davis, California 95616, United States.
authors.forename: Joan G
authors.initials: JG
authors.lastname: Schellinger
authors.forename: Lieza M
authors.initials: LM
authors.lastname: Danan-Leon
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Hoch
authors.forename: Aemro
authors.initials: A
authors.lastname: Kassa
authors.forename: Indresh
authors.initials: I
authors.lastname: Srivastava
authors.forename: David
authors.initials: D
authors.lastname: Davis
authors.forename: Jacquelyn
authors.initials: J
authors.lastname: Gervay-Hague
mesh_terms: D000595:Amino Acid Sequence; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D006684:HLA-DR Antigens / Q000737:chemistry* / Q000276:immunology; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology; D017780:Malaria Vaccines / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D018984:Epitopes, T-Lymphocyte; D015699:HIV Envelope Protein gp120; D006684:HLA-DR Antigens; D016056:Immunodominant Epitopes; D017780:Malaria Vaccines; C401223:PADRE 45; D010455:Peptides; C514793:gp120 protein, Human immunodeficiency virus 1