title: HLA-DRBeta1, circulating Th1/Th2 cytokines and immunological homunculus in coronary atherosclerosis.
abstract: Coronary atherosclerotic disease is one of the most endangering health disorder worldwide. This study was designed to investigate the correlation between HLA-DR1 alleles and circulating Th1/Th2 type cytokines in coronary atherosclerosis. By Elisa, Th1/Th2 type cytokines were determined in serum samples of 31 subjects with unstable angina, 27 subjects with chronic stable angina and 24 individuals as normal control. By SSP-PCR, more than 100 alleles of HLA-DRBeta1 were typed in 24 subjects who had skewed serum levels of Th1/Th2 type cytokines. Lipid profiles were determined by the routine methods of clinical laboratory in all subjects. The mean serum concentration of IL-10 in normal control subjects was higher in comparison to the patient groups.0.33±0.59 pg/ml versus 0.064±0.3 pg/ml in unstable angina pectoris group (p<0.028) and 0.22±0.6 pg/ml in chronic stable subjects. There was no statistically significant difference among the groups in serum levels of other desired cytokines (IFN-Gamma, IL-4). 33.33% of normal control subjects were HLA-DR16 positive whereas none of the subjects with chronic stable angina or individuals with unstable angina pectoris was positive for this antigen. The mean concentration of serum LDL-cholesterol in normal control group was high 142.046±35.40 (pg/ml).This preliminary study shows that the atherogenic effect of the LDL- cholesterol may be dampened by HDL-cholesterol through anti inflammatory cytokine IL-10 and HLA-DR16, a phenomenon interpretable via immunological homunculus theory.
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. amrolah65@yahoo.com
authors.forename: Amrollah
authors.initials: A
authors.lastname: Mostafazadeh
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Saravi
authors.forename: Haleh Akhavan
authors.initials: HA
authors.lastname: Niaki
authors.forename: Jos
authors.initials: J
authors.lastname: Drabbels
authors.forename: Haji Mohammad
authors.initials: HM
authors.lastname: Gholipour
authors.forename: Milad
authors.initials: M
authors.lastname: Minagar
authors.forename: Ezatola
authors.initials: E
authors.lastname: Mosavi
authors.forename: Farzad
authors.initials: F
authors.lastname: Jalali
authors.forename: Ali
authors.initials: A
authors.lastname: Bijani
mesh_terms: D000328:Adult; D000368:Aged; D000787:Angina Pectoris / Q000235:genetics / Q000276:immunology; D000789:Angina, Unstable / Q000235:genetics / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000150:complications / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D059811:HLA-DRB1 Chains; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D007492:Iran; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018570:Risk Assessment; D012307:Risk Factors; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D016207:Cytokines; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C067266:HLA-DR16 antigen; D059811:HLA-DRB1 Chains; C508609:IL10 protein, human; D016753:Interleukin-10