title: Liquid phase deposition of hemoglobin/SDS/TiO2 hybrid film preserving photoelectrochemical activity.
abstract: This work demonstrates that liquid phase deposition (LPD) technique provides a novel approach to the immobilization of hemoglobin (Hb) in TiO(2) film for studying the direct electron transfer of Hb. Using the LPD process, a hybrid film composed of Hb, TiO(2) and sodium dodecylsulfonate (SDS) is successfully prepared on the electrode surface. The surface morphology of as-deposited Hb/SDS/TiO(2) film shows a flower-like structure. The cyclic voltammetric measurement indicates that the LPD hybrid film facilitates the electron transfer of Hb, which yields a pair of redox peaks prior to the characteristic voltammetric peaks of TiO(2). Due to the electrocatalytic activity of Hb towards H(2)O(2), the Hb/SDS/TiO(2) hybrid LPD film can be utilized as an H(2)O(2) sensor, showing a sensitive response linearly proportional to the concentration of H(2)O(2) in the range of 5.0×10(-7)-4.0×10(-5) mol/L. At the same time, the Hb/SDS/TiO(2) hybrid film preserves the photoelectrochemical activity of TiO(2). The photovoltaic effect on the electrochemical behavior of Hb/SDS/TiO(2) film is observed after long-time UV irradiation on the film, which could improve the calibration sensitivity for H(2)O(2).
authors.affiliation: College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan 430074, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Jingdong
authors.initials: J
authors.lastname: Zhang
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D004595:Electroplating; D006454:Hemoglobins / Q000737:chemistry* / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry* / Q000528:radiation effects; D007700:Kinetics; D010084:Oxidation-Reduction; D011791:Quartz / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry* / Q000528:radiation effects; D013053:Spectrophotometry; D014025:Titanium / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D006454:Hemoglobins; D055767:Immobilized Proteins; D011791:Quartz; C009495:titanium dioxide; D012967:Sodium Dodecyl Sulfate; D006861:Hydrogen Peroxide; D014025:Titanium