title: Microparticle and cell counting with digital microfluidic compact disc using standard CD drive.
abstract: Lab-on-chip medical diagnostics in a global health setting would greatly benefit from highly portable, cost effective and readily available devices. Digital compact disc (CD) and the corresponding detection device-CD drives-for personal computers are extremely affordable and distributable worldwide, therefore they can be immediately used in global health applications if empowered with molecular and cellular biosensing functions. Here we present a novel digital microfluidic CD device derived from conventional music or data CD and demonstrate its preliminary application of counting polystyrene microparticles and living cells in minute-volume fluidic samples. No other detection instruments except for a standard CD drive in a personal computer is used for reading and decoding the quantitative liquid sample information from the digital microfluidic CD. The results presented herein are the first step towards creating a truly portable, low-cost and ubiquitously accessible device-health diagnostic compact disc (HDCD)-for biosensing and health diagnostics, especially in remote or impoverished settings with limited medical infrastructure and healthcare workers.
authors.affiliation: Department of Electrical & Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Syed M
authors.initials: SM
authors.lastname: Imaad
authors.forename: Nathan
authors.initials: N
authors.lastname: Lord
authors.forename: Gulsim
authors.initials: G
authors.lastname: Kulsharova
authors.forename: Gang Logan
authors.initials: GL
authors.lastname: Liu
mesh_terms: D000818:Animals; D016466:CHO Cells; D002452:Cell Count / Q000295:instrumentation*; D002470:Cell Survival; D016250:Compact Disks; D003201:Computers; D006224:Cricetinae; D003412:Cricetulus; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011076:Polycarboxylate Cement / Q000737:chemistry; D013499:Surface Properties
chemical_list: D011076:Polycarboxylate Cement; C005506:polycarbonate