title: 1H, 15N and 13C resonance assignment of the N-terminal C39 peptidase-like domain of the ABC transporter Haemolysin B (HlyB).
abstract: ATP-binding cassette (ABC) transporters are ubiquitous integral membrane proteins, which catalyze the translocation of molecules across biological membranes in an ATP-dependent manner. Despite the diversity in the transported substrates, they all share the same architecture, comprised of two transmembrane (TMD) and two nucleotide-binding domains (NBD). Members of the bacteriocin ABC transporter subfamily feature a special domain, belonging to the C39 (cystein protease family 39) peptidase protein family. These domains are assumed to cleave a C-terminal signal sequence from the protein or peptide substrate before or during the transport process. Although the C39 peptidase-like domain of the ABC transporter haemolysin B from E. coli shows no proteolytic activity, it is essential for the function of this transporter. In order to elucidate the contribution of the isolated C39 peptidase-like domain in the whole transport process, the backbone and side chain (1)H, (15)N and (13)C-NMR chemical shifts have been assigned.
authors.affiliation: Institute of Structural Biochemistry (ICS-6), Research Centre Juelich, Juelich, Germany.
authors.forename: Justin
authors.initials: J
authors.lastname: Lecher
authors.forename: Matthias
authors.initials: M
authors.lastname: Stoldt
authors.forename: Christian K W
authors.initials: CK
authors.lastname: Schwarz
authors.forename: Sander H J
authors.initials: SH
authors.lastname: Smits
authors.forename: Lutz
authors.initials: L
authors.lastname: Schmitt
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D020134:Catalytic Domain; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry*; D007554:Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D007554:Isotopes; C086257:hemolysin B