title: Gene knockdown in planarians using RNA interference.
abstract: INTRODUCTIONThis protocol describes how to produce gene knockdown in planarians using RNA interference (RNAi). It is a standard technique to evaluate gene function during regeneration and tissue maintenance in planarians. The procedure involves microinjecting double-stranded RNA (dsRNA) synthesized in vitro. Depending on the gene target, this technique can produce robust phenotypes that can be further evaluated by diverse macroscopic or microscopic procedures.
authors.affiliation: Center for Regenerative and Developmental Biology, Forsyth Institute and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA.
authors.forename: Néstor J
authors.initials: NJ
authors.lastname: Oviedo
authors.forename: Cindy L
authors.initials: CL
authors.lastname: Nicolas
authors.forename: Dany S
authors.initials: DS
authors.lastname: Adams
authors.forename: Michael
authors.initials: M
authors.lastname: Levin