title: Cryptosporidium pig genotype II diagnosed in pigs from the state of Rio De Janeiro, Brazil.
abstract: Pigs may represent a source of Cryptosporidium sp. infection to humans. The objective of this study was to identify the Cryptosporidium species present in pigs from the State of Rio de Janeiro, Brazil, and verify what risks pigs represent in the transmission of human cryptosporidiosis, because there is no such information to date in Brazil. Ninety-one samples of pig feces were collected from 10 piggeries in 2 municipalities located in the north and northwest regions of the State of Rio de Janeiro, Brazil. A nested polymerase chain reaction (PCR) protocol to amplify an 830-bp fragment of the small subunit rDNA (SSU rRNA) gene was followed by sequencing of all positive PCR samples. Two samples (2.2%) were Cryptosporidium sp. positive and were identified as pig genotype type II (PGII). This genotype has been observed in an immunocompetent person, in cattle without pigs nearby, and from a potential human source. Its potential for zoonotic transmission is little known and should be rigorously studied.
authors.affiliation: Laboratório de Clínica e Cirurgia Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro 28013-023, Brazil.
authors.forename: V R S
authors.initials: VR
authors.lastname: Fiuza
authors.forename: S S M
authors.initials: SS
authors.lastname: Gallo
authors.forename: E
authors.initials: E
authors.lastname: Frazão-Teixeira
authors.forename: M
authors.initials: M
authors.lastname: Santín
authors.forename: R
authors.initials: R
authors.lastname: Fayer
authors.forename: F C R
authors.initials: FC
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012307:Risk Factors; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission
chemical_list: D016054:DNA, Protozoan