title: Characterization of a novel temperate phage originating from a cereulide-producing Bacillus cereus strain.
abstract: A novel temperate bacteriophage was isolated from a Bacillus cereus cereulide-producing strain and named vB_BceS-IEBH. vB_BceS-IEBH belongs to the Siphoviridae family. The complete genome sequence (53 kb) was determined and annotated. Eighty-seven ORFs were detected and for 28, a putative function was assigned using the ACLAME database. vB_BceS-IEBH replicates as a plasmid in the prophage state. Accordingly, a 9-kb plasmid-like region composed of 13 ORFs was identified. A fragment of around 2000 bp comprising an ORF encoding a putative plasmid replication protein was shown to be self-replicating in Bacillus thuringiensis. Mass spectrometry analysis of the purified vB_BceS-IEBH particle identified 8 structural proteins and enabled assignment of a supplementary ORF as being part of the morphogenesis module. Genome analysis further illustrates the diversity of mobile genetic elements and their plasticity within the B. cereus group.
authors.affiliation: Laboratoire de Génétique et Physiologie Bactérienne, IBMM, Faculté des Sciences, Université Libre de Bruxelles, 12 Rue des Professeurs, Jeener et Brachet, 6041 Gosselies, Belgium.
authors.forename: Pierre R
authors.initials: PR
authors.lastname: Smeesters
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Drèze
authors.forename: Sabrina
authors.initials: S
authors.lastname: Bousbata
authors.forename: Kaarle J
authors.initials: KJ
authors.lastname: Parikka
authors.forename: Sophie
authors.initials: S
authors.lastname: Timmery
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Hu
authors.forename: David
authors.initials: D
authors.lastname: Perez-Morga
authors.forename: Marie
authors.initials: M
authors.lastname: Deghorain
authors.forename: Ariane
authors.initials: A
authors.lastname: Toussaint
authors.forename: Jacques
authors.initials: J
authors.lastname: Mahillon
authors.forename: Laurence
authors.initials: L
authors.lastname: Van Melderen
mesh_terms: D017103:Bacillus Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D047630:Depsipeptides / Q000378:metabolism*; D016679:Genome, Viral; D016366:Open Reading Frames; D039002:Prophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D047630:Depsipeptides; C089294:cereulide