title: Graft-transmitted siRNA signal from the root induces visual manifestation of endogenous post-transcriptional gene silencing in the scion.
abstract: In plants, post-transcriptional gene silencing (PTGS) spreads systemically, being transmitted from the silenced stock to the scion expressing the corresponding transgene. It has been reported that a graft-transmitted siRNA signal can also induce PTGS of an endogenous gene, but this was done by top-grafting using silenced stock. In the present study involving grafting of Nicotiana benthamiana, we found that PTGS of an endogenous gene, glutamate-1-semialdehyde aminotransferase (GSA), which acts as a visible marker of RNAi via inhibition of chlorophyll synthesis, was manifested along the veins of newly developed leaves in the wild-type scion by the siRNA signal synthesized only in companion cells of the rootstock.
authors.affiliation: Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasai
authors.forename: Songling
authors.initials: S
authors.lastname: Bai
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Li
authors.forename: Takeo
authors.initials: T
authors.lastname: Harada
mesh_terms: D001947:Breeding; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D019751:Intramolecular Transferases / Q000235:genetics; D018517:Plant Roots / Q000235:genetics*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D034741:RNA, Small Interfering; D019751:Intramolecular Transferases; C061049:glutamate-1-semialdehyde 2,1-aminomutase