title: [NMR-spectroscopy-based metabonomic study on mouse model of blood deficiency syndrome induced by compound method of bleeding, starved feeding and exhausting and effect of siwutang].
abstract: OBJECTIVE
  To explore the essence of blood deficiency syndrome and the mechanism of the effects of siwutang based on the characteristic changes in metabolic profiles of C57 mice with blood deficiency induced by compound method of bleeding, starved feeding and exhausting before and after the treatment of Siwutang.
  
  
  METHODS
  Thirty C57 mice were randomly divided into three groups of 10 animals: the control group and two blood deficiency model groups without or with siwutang treatment. The animals were sacrificed after induction by compound method of bleeding,starved feeding and exhausting for 10 days and the blood, spleen, thymus and bone marrow were obtained. The metabolic profiles of serum, aqueous and lipidic extracts of thymus, spleen and bone marrow were investigated using 1H nuclear magnetic resonance spectroscopy (NMR). The NMR spectra were integrated in segments of 0.01, normalized and then analyzed using SIMCA-P software to visualize the similarities and differences in metabolic profiles between these groups.
  
  
  RESULTS
  Compared with the control group, the model group contained different levels of lactate, choline, glucose, taurine, alanine, LDL, glycerin, UFA, creatinine, acetoacetate, glutamate and beta-hydroxybutyrate (beta-HB). In the Siwutang treated group, the above-mentioned changes were reversed.
  
  
  CONCLUSIONS
  The disorder of energy metabolism, damage of lymphocyte, and disorder of immune function were observed in blood deficiency model by NMR-based-metabonomics method, and the Siwutang can improve these effects. Metabonomics may be a valuable tool in the pharmacological evaluation of siwutang.
authors.affiliation: Pharmacol and Toxicol Department, Beijing Institute of Radiation Medicine, BeifJing 100850, China. iamhc2009@163.com
authors.forename: Chao
authors.initials: C
authors.lastname: Huo
authors.forename: Zengchun
authors.initials: Z
authors.lastname: Ma
authors.forename: Mu
authors.initials: M
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Yan
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006470:Hemorrhage / Q000150:complications* / Q000188:drug therapy* / Q000209:etiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D051379:Mice; D011897:Random Allocation; D044967:Serum / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal