title: Genotyping by Oligonucleotide Ligation Assay (OLA).
abstract: INTRODUCTIONThis protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane arrays using radiolabeled probes.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Macdonald