title: Multiscale fabrication of multiple proteins and topographical structures by combining capillary force lithography and microscope projection photolithography.
abstract: We present new methods that enable the fabrication of multiscale, multicomponent protein-patterned surfaces and multiscale topologically structured surfaces by exploiting the merits of two well-established techniques: capillary force lithography (CFL) and microscope projection photolithography (MPP) based on a protein-friendly photoresist. We further demonstrate that, when hierarchically organized micro- and nanostructures were used as a cell culture platform, human colon cancer cells (cell line SW480) preferentially adhere and migrate onto the area with nanoscale topography over the one with microscale topography. These methods will provide many exciting opportunities for the study of cellular responses to multiscale physicochemical cues.
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Keon Woo
authors.initials: KW
authors.lastname: Kwon
authors.forename: Jong-Cheol
authors.initials: JC
authors.lastname: Choi
authors.forename: Kahp-Yang
authors.initials: KY
authors.lastname: Suh
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins