title: [Chemotaxis-related factors are expressed abnormally in bone marrow mesenchymal stem cells of multiple myeloma patients].
abstract: This study was aimed to investigate the mRNA expression levels of hepatocyte growth factor (HGF), stromal cell-derived factor-1 (SDF-1), monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in bone marrow mesenchymal stem cells (MSC) from multiple myeloma (MM) patients. The mRNA expression levels of HGF, SDF-1, MCP-1 and IL-8 in bone marrow MSC from 20 newly diagnosed MM patients were detected by real time quantitative RT-PCR and were compared with that in 9 controls. The results indicated that the mean mRNA expression level of HGF was up-regulated in MM patients, as compared with controls (p < 0.01). However, the mean mRNA expression level of SDF-1 mRNA was down-regulated in MM patients, as compared with controls (p < 0.05). There was no significant difference in the mRNA expression levels of MCP-1 and IL-8 between MM and control cohorts (p > 0.05). It is concluded that BM-MSC from MM patients express HGF, SDF-1, MCP-1, IL-8, but these chemotaxis-related factors expression of bone marrow microenvironment cellular component are dysregulated in MM patients, which may result from the interplay between MM cells and MSC.
authors.affiliation: Department of Hematology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Hui-Jin
authors.initials: HJ
authors.lastname: Hu
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Fei
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Li
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism*; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism; D002630:Chemotactic Factors / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009101:Multiple Myeloma / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018932:Chemokine CCL2; D054377:Chemokine CXCL12; D002630:Chemotactic Factors; D016209:Interleukin-8; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor