title: Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent.
abstract: INTRODUCTIONThis protocol describes a method for quantitating the amount of free thiols and disulfide bond linkages in proteins. It uses the sulfhydryl reagent 5,5'-dithiobis (2-nitrobenzoic acid), or DTNB, which is also referred to as Ellman's reagent.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson