title: Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium: involvement of versatile monooxygenase.
abstract: To elucidate functional diversity of cytochrome P450 monooxygenases from the white-rot basidiomycete Phanerochaete chrysosporium (PcCYPs), we conducted a comprehensive functional screening using a wide variety of compounds. A functionomic survey resulted in characterization of novel PcCYP functions and discovery of versatile PcCYPs that exhibit broad substrate profiles. These results suggested that multifunctional properties of the versatile PcCYPs would play crucial roles in diversification of fungal metabolic systems involved in xenobiotic detoxification. To our knowledge, this is the first report describing multifunctional properties of versatile P450s from the fungal kingdom. An increased compilation of PcCYP functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.
authors.affiliation: Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Hirosue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tazaki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hiratsuka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yanai
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kabumoto
authors.forename: Raku
authors.initials: R
authors.lastname: Shinkyo
authors.forename: Akira
authors.initials: A
authors.lastname: Arisawa
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunekawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Johdo
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ichinose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
mesh_terms: D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D010802:Phylogeny; D013379:Substrate Specificity
chemical_list: D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases