title: Antioxidant effect of pomegranate extract in reducing acute inflammation due to myringotomy.
abstract: OBJECTIVE
  To assess the effect of pomegranate extract on acute inflammation due to myringotomy.
  
  
  METHODS
  Prospective, randomised study.
  
  
  METHODS
  Thirty Sprague-Dawley rats were divided into three groups. Group one constituted controls. Group two underwent myringotomy. Group three underwent myringotomy and also received 100 µl/day pomegranate extract, via gavage, one day before and two days after surgery. Following sacrifice 48 hours after myringotomy, the animals' right ears were used to determine the concentration of reactive oxygen species, using the chemiluminescence method; left ears were used for histological study.
  
  
  RESULTS
  Reactive oxygen species levels were significantly decreased in group three compared with group two (p < 0.01). The density of inflammatory cells in group three was significantly less than that in group two (p < 0.01). Lamina propria thickness and vessel density were also significantly decreased in group three compared with group two (p < 0.01).
  
  
  CONCLUSIONS
  Our results indicate that oral pomegranate extract decreases reactive oxygen species concentration and acute inflammation in the tympanic membrane after myringotomy.
authors.affiliation: Otorhinolaryngology Department, Bezmialem Vakif University Medical Faculty, Istanbul, Turkey.
authors.forename: V
authors.initials: V
authors.lastname: Kahya
authors.forename: A
authors.initials: A
authors.lastname: Meric
authors.forename: M
authors.initials: M
authors.lastname: Yazici
authors.forename: M
authors.initials: M
authors.lastname: Yuksel
authors.forename: A
authors.initials: A
authors.lastname: Midi
authors.forename: O
authors.initials: O
authors.lastname: Gedikli
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D049449:Luminescence; D029561:Lythraceae; D009225:Myringoplasty / Q000009:adverse effects*; D010032:Otitis Externa / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D014432:Tympanic Membrane / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species