title: A primer to scaffolded DNA origami.
abstract: Molecular self-assembly with scaffolded DNA origami enables building custom-shaped nanometer-scale objects with molecular weights in the megadalton regime. Here we provide a practical guide for design and assembly of scaffolded DNA origami objects. We also introduce a computational tool for predicting the structure of DNA origami objects and provide information on the conditions under which DNA origami objects can be expected to maintain their structure.
authors.affiliation: Center for Integrated Protein Science Munich, Physics Department and Walter Schottky Institut, Technische Universität München, Garching, Germany.
authors.forename: Carlos Ernesto
authors.initials: CE
authors.lastname: Castro
authors.forename: Fabian
authors.initials: F
authors.lastname: Kilchherr
authors.forename: Do-Nyun
authors.initials: DN
authors.lastname: Kim
authors.forename: Enrique Lin
authors.initials: EL
authors.lastname: Shiao
authors.forename: Tobias
authors.initials: T
authors.lastname: Wauer
authors.forename: Philipp
authors.initials: P
authors.lastname: Wortmann
authors.forename: Mark
authors.initials: M
authors.lastname: Bathe
authors.forename: Hendrik
authors.initials: H
authors.lastname: Dietz
mesh_terms: D017076:Computer-Aided Design; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D017931:DNA Primers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation
chemical_list: D017931:DNA Primers; D004247:DNA