title: Spectrofluorimetric determination of SN-38, a promising new anti-tumor agent, in the presence and absence of organized media.
abstract: This study focuses on the spectrofluorimetric behavior of the camptothecin derivative 7-ethyl-10-hydroxycamptothecin (SN-38) alone and in the presence of organized media and also on its potential analytical applications. SN-38 displays native fluorescence in both lactone and carboxylate form, which has been the base for development of two spectrofluorimetric methods, one for the lactone form (acidic media) and another for the carboxylate form (basic media). In an attempt to improve the understanding of SN-38, its interaction with several cyclodextrins and surfactants has been studied using spectrofluorimetry. Consequently, the optimal working conditions for the determination of SN-38 have been established in both the presence and the absence of organized media. The proposed methods were applied to human urine, using liquid-liquid extraction for clean-up of the samples, with satisfactory recoveries. No interference of the urine matrix was observed.
authors.affiliation: Department of Analytical Chemistry, University of Extremadura, 06006 Badajoz, Spain. maribelro@unex.es
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Rodríguez-Cáceres
authors.forename: Diego
authors.initials: D
authors.lastname: Bohoyo Gil
authors.forename: Isabel
authors.initials: I
authors.lastname: Durán-Merá
authors.forename: Maria C
authors.initials: MC
authors.lastname: Hurtado Sánchez
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000652:urine*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D003505:Cyclodextrins / Q000737:chemistry; D006801:Humans; D000077146:Irinotecan; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D003505:Cyclodextrins; D013501:Surface-Active Agents; D000077146:Irinotecan; D002166:Camptothecin