title: Chungtaejeon, a Korean fermented tea, scavenges oxidation and inhibits cytokine induced proliferation and migration of human aortic smooth muscle cells.
abstract: Oxidation susceptibility of serum lipid and the proliferation and migration of vascular smooth muscle cells (VSMC) from tunica media to the sub endothelial region are the key steps in the progression of atherosclerosis. The objective of this study was to determine the effects of Chungtaejeon (CTJ) on oxidation and cytokine induced proliferation and migration of human aortic smooth muscle cells (HASMC). The antioxidative effects of CTJ were evaluated by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging assay, nitric oxide (NO) scavenging assay and thiobarbituric acid reactive substance (TBARS) assay. Similarly, the proliferation, migration and expression of matrix metalloproteinases (MMPs) in HASMC were assessed by MTT assay, transwell Boyden chamber assay and gelatin zymography, respectively. Western blotting was done to determine the protein expression of MMP-9, phospho extracellular regulated kinase (pERK1/2) and phospho c-Jun N-terminal kinase (pJNK). In results, the IC(50) values for DPPH and NO scavenging activities were 8.91 μg/ml and 14.32 μg/ml, respectively. Furthermore, CTJ inhibited TBARS formation dose dependently. The pretreatment of CTJ dose dependently inhibited the tumor necrosis factor-α (TNF-α) induced proliferation and MMP-9 expression and platelet derived growth factor (PDGF) induced migration of HASMC. Thus, CTJ can be suggested to have beneficial effect in the prevention of atherosclerosis.
authors.affiliation: Department of Oriental Medicine Resources, Mokpo National University, Mokpo, South Korea. raju_peace@yahoo.com
authors.forename: Rajendra
authors.initials: R
authors.lastname: Karki
authors.forename: Navin
authors.initials: N
authors.lastname: Sahi
authors.forename: Eun-Raye
authors.initials: ER
authors.lastname: Jeon
authors.forename: Yong-Seo
authors.initials: YS
authors.lastname: Park
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
mesh_terms: D001011:Aorta / Q000166:cytology; D001713:Biphenyl Compounds; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005285:Fermentation; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010851:Picrates; D013662:Tea / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001713:Biphenyl Compounds; D016207:Cytokines; D016166:Free Radical Scavengers; D010851:Picrates; D013662:Tea; D014409:Tumor Necrosis Factor-alpha; C004931:1,1-diphenyl-2-picrylhydrazyl; D020780:Matrix Metalloproteinase 9