title: Direct analysis of methylated trivalent arsenicals in mouse liver by hydride generation-cryotrapping-atomic absorption spectrometry.
abstract: Growing evidence suggest that the methylated trivalent metabolites of inorganic arsenic (iAs), methylarsonite (MAs(III)) and dimethylarsinite (DMAs(III)), contribute to adverse effects of iAs exposure. However, the lack of suitable methods has hindered the quantitative analysis of MAs(III) and DMAs(III) in complex biological matrices. Here, we show that hydride generation-cryotrapping-atomic absorption spectrometry can quantify both MAs(III) and DMAs(III) in livers of mice exposed to iAs. No sample extraction is required, thus limiting MAs(III) or DMAs(III) oxidation prior to analysis. The limits of detection are below 6 ng As/g of tissue, making this method suitable even for studies examining low exposures to iAs.
authors.affiliation: Curriculum in Toxicology, University of North Carolina at Chapel Hill, NC, USA.
authors.forename: Jenna M
authors.initials: JM
authors.lastname: Currier
authors.forename: Milan
authors.initials: M
authors.lastname: Svoboda
authors.forename: Diogo P
authors.initials: DP
authors.lastname: de Moraes
authors.forename: Tomás
authors.initials: T
authors.lastname: Matousek
authors.forename: Jirí
authors.initials: J
authors.lastname: Dĕdina
authors.forename: Miroslav
authors.initials: M
authors.lastname: Stýblo
mesh_terms: D000818:Animals; D001151:Arsenic / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D002101:Cacodylic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D051379:Mice; D010084:Oxidation-Reduction; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; C472511:dimethylarsinous acid; C501996:methylarsonite; D002101:Cacodylic Acid; D001151:Arsenic