title: The protective effect of Cu/Zn-SOD against oxidative stress after PTEN deletion.
abstract: Reactive oxygen species (ROS), antioxidant enzyme, and cancerization are related to each other. To investigate whether these factors are involved in the mechanism by which phosphatase and tensin homolog deleted on chromosome ten (PTEN) suppresses tumors. Fluorescent probes and molecular hybridizations were performed to measure ROS and antioxidant enzyme levels in cells lacking PTEN. After induction of oxidative stress by PTEN deletion, we observed an increase in the expression of the antioxidant enzyme Cu/Zn-SOD. In addition, the overall antioxidative capability of the cells was attenuated. These results suggest that negative regulation occurs through interactions among ROS, Cu/Zn-SOD, and PTEN, and such a disequilibrium between oxidation and antioxidation could contribute to the reduced antioxidative capacity in PTEN null cells. These studies will guide further investigations into the biological function of PTEN.
authors.affiliation: College of Medicine, Qingdao University, Qingdao, Shandong Province, China. yuchqd@gmail.com
authors.forename: Changhong
authors.initials: C
authors.lastname: Yu
authors.forename: Peilin
authors.initials: P
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.forename: Xiuhai
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenlei
authors.initials: Z
authors.lastname: Yu
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D005347:Fibroblasts / Q000201:enzymology* / Q000473:pathology; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress; D051059:PTEN Phosphohydrolase / Q000172:deficiency* / Q000235:genetics; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D013481:Superoxides / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D013481:Superoxides; D006861:Hydrogen Peroxide; C000606291:Sod1 protein, mouse; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse