title: Nondenaturing protein immunoprecipitation from Mammalian cells.
abstract: INTRODUCTIONThis protocol describes a nondenaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. However, the nondenaturing protocol is useful when one wishes to separate soluble from insoluble proteins, or when the antibody being used recognizes a native epitope.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: William P
authors.initials: WP
authors.lastname: Tansey