title: Differential muscle gene expression as a function of disease progression in Goto-Kakizaki diabetic rats.
abstract: The Goto-Kakizaki (GK) rat, a polygenic non-obese model of type 2 diabetes, is a useful surrogate for study of diabetes-related changes independent of obesity. GK rats and appropriate controls were killed at 4, 8, 12, 16 and 20 weeks post-weaning and differential muscle gene expression along with body and muscle weights, plasma hormones and lipids, and blood cell measurements were carried out. Gene expression analysis identified 204 genes showing 2-fold or greater differences between GK and controls in at least 3 ages. Array results suggested increased oxidative capacity in GK muscles, as well as differential gene expression related to insulin resistance, which was also indicated by HOMA-IR measurements. In addition, potential new biomarkers in muscle gene expression were identified that could be either a cause or consequence of T2DM. Furthermore, we demonstrate here the presence of chronic inflammation evident both systemically and in the musculature, despite the absence of obesity.
authors.affiliation: Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA. jnie2@buffalo.edu
authors.forename: Jing
authors.initials: J
authors.lastname: Nie
authors.forename: Bai
authors.initials: B
authors.lastname: Xue
authors.forename: Siddharth
authors.initials: S
authors.lastname: Sukumaran
authors.forename: William J
authors.initials: WJ
authors.lastname: Jusko
authors.forename: Debra C
authors.initials: DC
authors.lastname: Dubois
authors.forename: Richard R
authors.initials: RR
authors.lastname: Almon
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001786:Blood Glucose; D001837:Body Weights and Measures; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D004734:Energy Metabolism / Q000235:genetics; D005227:Fatty Acids / Q000097:blood; D020869:Gene Expression Profiling; D005796:Genes; D007249:Inflammation / Q000235:genetics; D007328:Insulin / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D051381:Rats; D011921:Rats, Inbred WKY; D055647:Rats, Transgenic; D015398:Signal Transduction / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D001786:Blood Glucose; D005227:Fatty Acids; D007328:Insulin