title: [Application of polymer detection systems of Histofine MOUSESTAIN, Mouse MAX-PO and rat MAX-PO for mouse and rat frozen tissue sections].
abstract: We defined the staining applicability of Histofine MOUSESTAIN, Mouse MAX-PO and Rat MAX-PO for fixed frozen and fresh frozen tissue sections besides paraffin embedded tissue sections. Two different types of cool air dried tissue sections, fresh frozen tissues and fixed frozen tissues of nine-week old Slc: ICR mouse and nine-week old Slc: SD rat, were prepared for immunohistochemistry (IHC) staining in accordance with conventional method. Additional two steps of 1 and 2 to our recommended procedure for paraffin embedded tissue sections were examined as follows: STEP 1: Adjust reaction time of polymer; STEP 2: Add 0.2% of glutaraldehyde (GA) solution before the STEP 1, when background staining is still observed by STEP 1. The steps 1 and 2 resulted in elimination of background staining of most Histofine series. Therefore, polymer detection systems, Histofine series, are applicable for IHC staining of two different types of frozen tissue sections as well as paraffin embedded tissue sections.
authors.affiliation: Research & Development Division, Nichirei Biosciences INC., Higashimurayama 189-0003, Japan. kasamatsut@nichirei.co.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kasamatsu
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Kitano
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ohbayashi
mesh_terms: D000818:Animals; D005629:Frozen Sections / Q000379:methods*; D005976:Glutaral; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016612:Paraffin Embedding / Q000379:methods*; D011108:Polymers; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D011108:Polymers; D005976:Glutaral