title: Marinobacter adhaerens sp. nov., isolated from marine aggregates formed with the diatom Thalassiosira weissflogii.
abstract: A Gram-negative, motile, rod-shaped bacterial strain, HP15(T), was isolated from aggregates taken from surface waters of the German Wadden Sea (German Bight). Of 82 marine isolates, HP15(T) was chosen for further study because of its high potential to induce production of transparent exopolymeric particles and aggregate formation while interacting with the diatom Thalassiosira weissflogii. HP15(T) grew optimally at 34-38 °C and pH 7.0-8.5, and was able to tolerate salt concentrations of 0.5-20% (w/v) NaCl. HP15(T) was characterized chemotaxonomically by possessing ubiquinone-9 as the major respiratory lipoquinone, as well as C(16:0), C(18:1)ω9c and C(16:1)ω7c/iso-C(15:0) 2-OH as the predominant fatty acids. The DNA G+C content of strain HP15(T) was 56.9 mol%. The closest relative based on 16S rRNA gene sequence analysis was the type strain of Marinobacter flavimaris, with 99% similarity. Whole-genome relatedness values of HP15(T) to the type strains of M. flavimaris, Marinobacter salsuginis, Marinobacter lipolyticus and Marinobacter algicola were less than 70%, as determined by DNA-DNA hybridization. On the basis of phenotypic and chemotaxonomic properties as well as phylogenetic analyses, the isolate represents a novel species, Marinobacter adhaerens sp. nov.; the type strain is HP15(T) (=DSM 23420(T)=CIP 110141(T)).
authors.affiliation: Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Eva C
authors.initials: EC
authors.lastname: Kaeppel
authors.affiliation: Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Gärdes
authors.affiliation: Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Shalin
authors.initials: S
authors.lastname: Seebah
authors.affiliation: Leibniz Institute of Freshwater Ecology and Inland Fisheries - Neuglobsow, Alte Fischerhütte 2, 16775 Stechlin, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Grossart
authors.affiliation: Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Matthias S
authors.initials: MS
authors.lastname: Ullrich
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017377:Diatoms / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride