title: Virus inactivation in albumin by a combination of alkali conditions and high temperature.
abstract: Non-enveloped viruses such as HAV and B19 are of potential concern in plasma products. In the case of albumin, pasteurisation at 60 °C for 10 h is generally used for virus inactivation. However this procedure is only partially effective against some non-enveloped viruses. Using a range of non-enveloped viruses i.e. HAV, SV40, CPV, treatment at a high pH of about 9.5 and a temperature of 60 °C for 10 h was found to be effective for virus inactivation. These extreme conditions caused no increase in aggregate composition of the albumin. In addition the albumin composition was stable over a period of at least 6 months. The ligand binding properties of the albumin, as determined using the dye phenol red, were also not affected by this treatment. This procedure has the potential for increasing the spectrum of viruses inactivated by the 60 °C pasteurisation step.
authors.affiliation: R&D Department, Bio Products Laboratory, Elstree, Hertfordshire WD6 3BX, UK. peter.roberts@bpl.co.uk
authors.forename: Peter L
authors.initials: PL
authors.lastname: Roberts
authors.forename: John
authors.initials: J
authors.lastname: More
authors.forename: Jackie
authors.initials: J
authors.lastname: Rott
authors.forename: David
authors.initials: D
authors.lastname: Lewin
mesh_terms: D000418:Albumins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000468:Alkalies / Q000494:pharmacology*; D000818:Animals; D052660:Bocavirus / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D030041:Hepatitis A virus / Q000187:drug effects / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017993:Parvovirus, Canine / Q000187:drug effects / Q000502:physiology; D055550:Protein Stability / Q000187:drug effects; D013539:Simian virus 40 / Q000187:drug effects / Q000502:physiology; D014709:Vero Cells; D038301:Virus Inactivation / Q000187:drug effects
chemical_list: D000418:Albumins; D000468:Alkalies