title: Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands.
abstract: The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH(2) stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.
authors.affiliation: University of California, Irvine, Department of Chemistry and Beckman Laser Institute, Irvine, California 92697, USA.
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Suhalim
authors.forename: Chyong Ly
authors.initials: CL
authors.lastname: Nien
authors.forename: Milos D
authors.initials: MD
authors.lastname: Miljković
authors.forename: Max
authors.initials: M
authors.lastname: Diem
authors.forename: James V
authors.initials: JV
authors.lastname: Jester
authors.forename: Eric O
authors.initials: EO
authors.lastname: Potma
mesh_terms: D000818:Animals; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008537:Meibomian Glands / Q000166:cytology*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*