title: Wavelength-selective uncaging of dA and dC residues.
abstract: Nitrodibenzofuran (NDBF) groups are used as photolabile "caging" groups to temporarily mask the Watson-Crick interaction of dA and dC residues. They show improved masking capabilities and are photodeprotected 12 times more efficiently than 1-(o-nitrophenyl)-ethyl (NPE) caging groups in these positions. Furthermore, NDBF groups can be removed wavelength-selectively in the presence of NPE groups. This will allow more complex (un)caging strategies of oligonucleotides--beyond the usual irreversible triggering.
authors.affiliation: Cluster of Excellence Macromolecular Complexes, Goethe University of Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt (M), Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Schäfer
authors.forename: Khashti Ballabh
authors.initials: KB
authors.lastname: Joshi
authors.forename: Manuela A H
authors.initials: MA
authors.lastname: Fichte
authors.forename: Timo
authors.initials: T
authors.lastname: Mack
authors.forename: Josef
authors.initials: J
authors.lastname: Wachtveitl
authors.forename: Alexander
authors.initials: A
authors.lastname: Heckel
mesh_terms: D001572:Benzofurans / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry* / Q000528:radiation effects; D055668:Photochemical Processes; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001572:Benzofurans; D009841:Oligonucleotides; C067509:3-nitrodibenzofuran; D004247:DNA