title: Zygocotyle lunata: proteomic analysis of the adult stage.
abstract: The somatic extract of Zygocotyle lunata (Trematoda: Paramphistomidae) adults collected from experimentally infected mice was investigated using a proteomic approach to separate and identify tryptic peptides from the somatic extract of Z. lunata adult worms. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 36 proteins were accurately identified from the worms. The largest protein family consisted of metabolic enzymes. Structural, motor and receptor binding proteins and proteins related to oxygen transport were identified in the somatic extract of Z. lunata. This is the first study that attempts to identify the proteome of Z. lunata. However, more work is needed to improve our knowledge of trematodiasis in general and more specifically to have a better understanding about host-parasite relationships in infections with paramphistomes.
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Sotillo
authors.forename: M Luz
authors.initials: ML
authors.lastname: Valero
authors.forename: Manuel M
authors.initials: MM
authors.lastname: Sánchez del Pino
authors.forename: Bernard
authors.initials: B
authors.lastname: Fried
authors.forename: J Guillermo
authors.initials: JG
authors.lastname: Esteban
authors.forename: Antonio
authors.initials: A
authors.lastname: Marcilla
authors.forename: Rafael
authors.initials: R
authors.lastname: Toledo
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D030562:Databases, Protein; D015801:Helminth Proteins / Q000032:analysis* / Q000235:genetics; D006790:Host-Parasite Interactions; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010250:Paramphistomatidae / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D020543:Proteome / Q000032:analysis* / Q000235:genetics; D053719:Tandem Mass Spectrometry; D014201:Trematode Infections / Q000469:parasitology*
chemical_list: D015801:Helminth Proteins; D020543:Proteome