title: Estrogen-dependent gene expression in the mouse ovary.
abstract: Estrogen (E) plays a pivotal role in regulating the female reproductive system, particularly the ovary. However, the number and type of ovarian genes influenced by estrogen remain to be fully elucidated. In this study, we have utilized wild-type (WT) and aromatase knockout (ArKO; estrogen free) mouse ovaries as an in vivo model to profile estrogen dependent genes. RNA from each individual ovary (n = 3) was analyzed by a microarray-based screen using Illumina Sentrix Mouse WG-6 BeadChip (45,281 transcripts). Comparative analysis (GeneSpring) showed differential expression profiles of 450 genes influenced by E, with 291 genes up-regulated and 159 down-regulated by 2-fold or greater in the ArKO ovary compared to WT. Genes previously reported to be E regulated in ArKO ovaries were confirmed, in addition to novel genes not previously reported to be expressed or regulated by E in the ovary. Of genes involved in 5 diverse functional processes (hormonal processes, reproduction, sex differentiation and determination, apoptosis and cellular processes) 78 had estrogen-responsive elements (ERE). These analyses define the transcriptome regulated by E in the mouse ovary. Further analysis and investigation will increase our knowledge pertaining to how E influences follicular development and other ovarian functions.
authors.affiliation: Prince Henry's Institute of Medical Research, Clayton, Melbourne, Australia. Jason.liew@princehenrys.org
authors.forename: Seng H
authors.initials: SH
authors.lastname: Liew
authors.forename: Mai A
authors.initials: MA
authors.lastname: Sarraj
authors.forename: Ann E
authors.initials: AE
authors.lastname: Drummond
authors.forename: Jock K
authors.initials: JK
authors.lastname: Findlay
mesh_terms: D000818:Animals; D001141:Aromatase / Q000172:deficiency / Q000235:genetics; D004967:Estrogens / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000254:growth & development / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics; D059467:Transcriptome
chemical_list: D004967:Estrogens; D001141:Aromatase