title: 3-Diethylaminopropyl-bearing glycol chitosan as a protein drug carrier.
abstract: Polysaccharidic nanogels were fabricated with bovine serum albumin (BSA) and a glycol chitosan (GCS) grafted with functional 3-diethylaminopropyl (DEAP) groups. These nanogels were investigated to evaluate their cellular uptake in HeLa cells and in vivo fate in nude mice tumor model. Unlike free BSA, GCS-g-DEAP/BSA nanogels improved cellular uptake of BSA. Furthermore, this system led to an enhanced blood circulation and a high accumulation of BSA in the tumor site. Our collective results strongly support that GCS-g-DEAP/BSA nanogel is a potential carrier system for high molecular weight proteins.
authors.affiliation: Division of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Hye Jung
authors.initials: HJ
authors.lastname: Baik
authors.forename: Nam Muk
authors.initials: NM
authors.lastname: Oh
authors.forename: Young Taik
authors.initials: YT
authors.lastname: Oh
authors.forename: Na Young
authors.initials: NY
authors.lastname: Yoo
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.forename: Kyung Taek
authors.initials: KT
authors.lastname: Oh
authors.forename: Yu Seok
authors.initials: YS
authors.lastname: Youn
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
mesh_terms: D000433:1-Propanol / Q000737:chemistry*; D000588:Amines / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008958:Models, Molecular; D015394:Molecular Structure; D000080385:Nanogels; D009369:Neoplasms / Q000188:drug therapy; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D000588:Amines; D000080385:Nanogels; C118638:glycol-chitosan; C413692:polyethylene glycol polyethyleneimine nanogel; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D048271:Chitosan; D000433:1-Propanol