title: Cell origin of human mesenchymal stem cells determines a different healing performance in cardiac regeneration.
abstract: The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment of myocardial infarction (MI) remains unexplored. This study was to assess the regenerative potential of hMSC from different origins and to evaluate the role of CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation and received the respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, cardiac catheterization showed significant preservation of left ventricular functions in BM and CD105(+)-CB treated groups compared to CB and nontreated MI group (MI-C). Cell survival analyzed by quantitative real time PCR for human GAPDH and capillary density measured by immunostaining showed consistent results. Furthermore, cardiac remodeling can be significantly attenuated by BM-hMSC compared to MI-C. Under hypoxic conditions in vitro, remarkably increased extracellular acidification and apoptosis has been detected from CB-hMSC compared to BM and CD105 purified CB-derived hMSC. Our findings suggests that hMSC originating from different sources showed a different healing performance in cardiac regeneration and CD105(+) hMSC exhibited a favorable survival pattern in infarcted hearts, which translates into a more robust preservation of cardiac function.
authors.affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Gaebel
authors.forename: Dario
authors.initials: D
authors.lastname: Furlani
authors.forename: Heiko
authors.initials: H
authors.lastname: Sorg
authors.forename: Bianca
authors.initials: B
authors.lastname: Polchow
authors.forename: Johannes
authors.initials: J
authors.lastname: Frank
authors.forename: Karen
authors.initials: K
authors.lastname: Bieback
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Christian
authors.initials: C
authors.lastname: Klopsch
authors.forename: Lee-Lee
authors.initials: LL
authors.lastname: Ong
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002196:Capillaries / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D006321:Heart / Q000503:physiopathology*; D006801:Humans; D008026:Ligation / Q000009:adverse effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D018919:Neovascularization, Physiologic; D011956:Receptors, Cell Surface / Q000378:metabolism; D012038:Regeneration; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014945:Wound Healing / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1