title: R453Plus1Toolbox: an R/Bioconductor package for analyzing Roche 454 Sequencing data.
abstract: The R453Plus1Toolbox is an R/Bioconductor package for the analysis of 454 Sequencing data. Projects generated with Roche's data analysis software can be imported into R allowing advanced and customized analyses within the R/Bioconductor environment for sequencing data. Several methods were implemented extending the current functionality of Roche's software. These extensions include methods for quality assurance and annotation of detected variants. Further, a pipeline for the detection of structural variants, e.g. balanced chromosomal translocations, is provided.
  
  
  BACKGROUND
  The R453Plus1Toolbox is implemented in R and available at http://www.bioconductor.org/. A vignette outlining typical workflows is included in the package.
  
  
  BACKGROUND
  h.klein@uni-muenster.de
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Department of Medical Informatics and Biomathematics, University of Münster, Münster and Munich Leukemia Laboratory, Munich, Germany. h.klein@uni-muenster.de
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Klein
authors.forename: Christoph
authors.initials: C
authors.lastname: Bartenhagen
authors.forename: Alexander
authors.initials: A
authors.lastname: Kohlmann
authors.forename: Vera
authors.initials: V
authors.lastname: Grossmann
authors.forename: Christian
authors.initials: C
authors.lastname: Ruckert
authors.forename: Torsten
authors.initials: T
authors.lastname: Haferlach
authors.forename: Martin
authors.initials: M
authors.lastname: Dugas
mesh_terms: D002874:Chromosome Mapping; D014644:Genetic Variation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software