title: Identification of mouse MARVELD1 as a microtubule associated protein that inhibits cell cycle progression and migration.
abstract: MARVEL domain-containing 1 (MARVELD1) is a newly identified nuclear protein; however its function has not been clear until now. Here, we report that mouse MARVELD1 (mMARVELD1), which is highly conserved between mice and humans, exhibits cell cycle-dependent cellular localization. In NIH3T3 cells, MARVELD1 was observed in the nucleus and at the perinuclear region during interphase, but was localized at the mitotic spindle and midbody at metaphase, and a significant fraction of mMARVELD1 translocated to the plasma membrane during anaphase. In addition, treatment of cells with colchicine, a microtubule-depolymerizing agent, resulted in translocation of mMARVELD1 to the plasma membrane, and association of mMARVELD1 and α-tubulin was confirmed by co-immunoprecipitation. Finally, overexpression of mMARVELD1 resulted in a remarkable inhibition of cell proliferation, G1-phase arrest, and reduced cell migration. These findings indicate that mMARVELD1 is a microtubule-associated protein that plays an important role in cell cycle progression and migration.
authors.affiliation: Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, 150001, People's Republic of China.
authors.forename: Fanli
authors.initials: F
authors.lastname: Zeng
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Tian
authors.forename: Shuliang
authors.initials: S
authors.lastname: Shi
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Yue
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D016384:Consensus Sequence; D016193:G1 Phase; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000378:metabolism*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000378:metabolism*
chemical_list: C561290:MARVELD1 protein, mouse; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D014404:Tubulin