title: [In vitro absorption mechanism of strychnine and the transport interaction with liquiritin in Caco-2 cell monolayer model].
abstract: To study the effect of liquiritin (Liq) on the transport of strychnine (Str) in Caco-2 cell monolayer model, the transport parameters of Str, such as apparent permeability coefficient (P app (B-->A) and P app (A-->B)) and cumulative transport amount (TRcum), were determined and comparatively analyzed when Str was used solely and co-used with Liq. The effect of drug concentrations, conveying times, P-glycoprotein (P-gp) inhibitor verapamil and conveying liquor pH values on the transport of Str were also investigated. The results indicated that the absorption of Str in Caco-2 cell monolayer model was well and the passive transference was the main intestinal absorption mechanism of Str in the Caco-2 monolayer model, along with the excretion action mediated by P-gp. Liq enhanced the absorption of Str. Meanwhile, conveying liquor pH value had significant influence on the excretion transport of Str.
authors.affiliation: Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei University, Wuhan 430062, China.
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Xiao-huan
authors.initials: XH
authors.lastname: Liao
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D000042:Absorption / Q000187:drug effects; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010539:Permeability; D010946:Plants, Medicinal / Q000737:chemistry; D013331:Strychnine / Q000302:isolation & purification / Q000493:pharmacokinetics*; D031461:Strychnos nux-vomica / Q000737:chemistry; D014700:Verapamil / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D044950:Flavanones; D005960:Glucosides; D014700:Verapamil; D013331:Strychnine; C512196:liquiritin