title: Construction of an effective screening system for detection of Pseudomonas aeruginosa quorum sensing inhibitors and its application in bioautographic thin-layer chromatography.
abstract: In Pseudomonas aeruginosa, quorum sensing (QS) regulates dozens of genes and proteins, many of which contribute to the virulence of this pathogen. QS inhibitory (QSI) compounds have been proposed as potential agents for treatment of bacterial infections. To search for Ps. aeruginosa QS inhibitors, we constructed an effective screening system, QSIS-lasI selector, based on the PlasI-sacB reporter, in which QS could be induced with 20 nM 3-oxo-N-[(3S)-tetrahydro-2-oxo-3-furanyl]-dodecanamide (3-oxo-C(12)-HSL). During screening of the crude extracts from 65 marine fungi, an isolate of Penicillium atramentosum was found to have QSI activity. Thin-layer chromatography assay of the fungal extracts for bioautographic identification of QSIS-lasI indicated that this fungus produced several QSI compounds, including QS inhibitors other than penicillic acid or patulin.
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, People's Republic of China. wlnwfllsy@163.com
authors.forename: Linna
authors.initials: L
authors.lastname: Wang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zou
authors.forename: Shouliang
authors.initials: S
authors.lastname: Yin
authors.forename: Hongbing
authors.initials: H
authors.lastname: Liu
authors.forename: Wengong
authors.initials: W
authors.lastname: Yu
authors.forename: Qianhong
authors.initials: Q
authors.lastname: Gong
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D001681:Biological Assay / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005658:Fungi / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D053038:Quorum Sensing / Q000187:drug effects*; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer