title: [Assessment of two commercially available immunochromatographic assays for a rapid diagnosis of Giardia duodenalis and Cryptosporidium spp. in human fecal specimens].
abstract: BACKGROUND
  To assess and compare the performance of two immunochromatographic tests for the simultaneous detection of Giardia duodenalis and Cryptosporidium spp. in faeces.
  
  
  METHODS
  In this study 254 faeces samples were tested using the two immunochromatography strips Cryto-Giardia (CerTest Biotec) and Stick Crypto-Giardia (Operon).
  
  
  RESULTS
  In the diagnosis of G. duodenalis, the sensitivity and specificity of the kits were 97% and 100%, respectively for the CerTest; and 97% and 95% for Operon. In the diagnosis of Cryptosporidium spp. Certest strip rendering a sensitivity of 100%, compared to with a sensitivity of 92% using Operon. There were no false positives using either technique.
  
  
  CONCLUSIONS
  Both methods yielded good sensitivity and specificity values and are thus useful tools for a rapid diagnosis of G. duodenalis and Cryptosporidium spp. The benefits of immunochromatography methods are that there is no requirement for expert microscopists or special equipment.
authors.affiliation: Servicio de Parasitología, Centro Nacional de Microbiología, ISCIII, Majadahonda, Madrid, España. mjguti@isciii.es
authors.forename: María José
authors.initials: MJ
authors.lastname: Gutiérrez-Cisneros
authors.forename: Rocío
authors.initials: R
authors.lastname: Martínez-Ruiz
authors.forename: Mercedes
authors.initials: M
authors.lastname: Subirats
authors.forename: Francisco Jesús
authors.initials: FJ
authors.lastname: Merino
authors.forename: Rosario
authors.initials: R
authors.lastname: Millán
authors.forename: Isabel
authors.initials: I
authors.lastname: Fuentes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002845:Chromatography / Q000379:methods*; D015897:Comorbidity; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D003458:Cryptosporidium / Q000276:immunology / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D016829:Giardia lamblia / Q000276:immunology / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D015658:HIV Infections / Q000453:epidemiology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D007223:Infant; D008297:Male; D008875:Middle Aged; D033661:Oocysts / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D011934:Reagent Strips