title: Study on protein conformation and adsorption behaviors in nanodiamond particle-protein complexes.
abstract: In the present research, the conformation of bovine serum albumin (BSA) in the nanodiamond particle (ND)-BSA complex was studied by Fourier transform infrared spectroscopy, fluorescence spectroscopy, UV-vis spectroscopy, and circular dichroism spectroscopy. The spectroscopic study revealed that most BSA structural features could be preserved in the complex though the BSA underwent conformational changes in the complex due to ND-BSA interaction. In addition, BSA adsorption isotherms and zeta-potential measurements were employed to investigate the pH dependence of the ND-BSA interaction. The changes in surface charge of the ND-BSA complex with pH variations indicated that the binding of BSA to ND might lead to not only the adsorption of BSA onto the ND surface but also the partial breakup of ND aggregates into relatively small ND-BSA aggregates because of the strong binding force between ND and BSA. The results show that ND is an excellent platform for protein immobilization with high affinity and holds great potential to be used for biosensor applications.
authors.affiliation: Department of Biomedical Engineering, University of Saskatchewan, Saskatoon S7N5A9, Canada.
authors.forename: Hai-Dong
authors.initials: HD
authors.lastname: Wang
authors.forename: Catherine Hui
authors.initials: CH
authors.lastname: Niu
authors.forename: Qiaoqin
authors.initials: Q
authors.lastname: Yang
authors.forename: Ildiko
authors.initials: I
authors.lastname: Badea
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods; D002942:Circular Dichroism; D018130:Diamond / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D055767:Immobilized Proteins; D011506:Proteins; D012710:Serum Albumin, Bovine; D018130:Diamond