title: [Effect of bilirubin on expression of toll-like receptor 4 in cord blood monocytes].
abstract: OBJECTIVE
  To study the effect of different concentrations of bilirubin on expression of toll-like receptor 4 (TLR4) in cord blood monocytes (CBMC).
  
  
  METHODS
  Under the sterile condition, umbilical vein blood samples were obtained from normal full-term newborns, and the monocytes were in vitro separated by the method of gelatin/plasma coated flasks. The monocytes were preincubated with various concentrations (0-307.8 μmol/L) of bilirubin dissolved in bovine albumin solution for 1 hr. Bilirubin-treated CBMC were further cultured with LPS (1 μg/mL) to induce cellular activation for 24 hrs, and then the CBMC were collected. The expression of TLR4 in monocytes was measured by indirect immunofluorescence method.
  
  
  RESULTS
  Bilirubin at the concentrations of 102.6, 153.9, 220.6 and 307.8 μmol/L inhibited the expression of TLR4 of CBMC. The inhibition effect increased with the increasing concentration of bilirubin.
  
  
  CONCLUSIONS
  Bilirubin can inhibit the TLR4 expression of CBNC in a dose-dependent manner.
authors.affiliation: Department of Pediatrics, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital, Chengdu 610072, China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Liu
authors.forename: Chang-Hui
authors.initials: CH
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.forename: Mao-Jun
authors.initials: MJ
authors.lastname: Li
mesh_terms: D001663:Bilirubin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005312:Fetal Blood / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D009000:Monocytes / Q000737:chemistry* / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000097:blood*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D001663:Bilirubin