title: Differential expression of anthocyanin biosynthetic genes and anthocyanin accumulation in tartary buckwheat cultivars 'Hokkai t8' and 'Hokkai t10'.
abstract: Six genes involved in anthocyanin biosynthesis in tartary buckwheat have been cloned, namely, FtC4H, Ft4CL, FtCHI, FtF3H, FtF3'H, and FtANS, which encode cinnamate 4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), chalcone isomerase (CHI), flavones 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), and anthocyanidin synthase (ANS), respectively. Then, these cDNAs were used, along with previously isolated clones for phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS), to compare gene expression in different organs, flowering stages, and maturing seeds of tartary buckwheat cultivars 'Hokkai T8' and 'Hokkai T10'. Quantitative real-time polymerase chain reaction analysis showed that these anthocyanin biosynthetic genes were most highly expressed in the stems and roots of Hokkai T10. The FtANS gene was more highly expressed than other genes during flowering and maturing seeds. In addition, the anthocyanin concentration was higher in 'Hokkai T10' than in 'Hokkai T8'; however, naringenin chalcone, a flavonoid, was absent from 'Hokkai T10' seedlings based on fluorescence microscopy.
authors.affiliation: Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon 305-764, Korea.
authors.forename: Nam Il
authors.initials: NI
authors.lastname: Park
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Sun-Ju
authors.initials: SJ
authors.lastname: Kim
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Woo
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Park
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D019612:Fagopyrum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D050564:Trans-Cinnamate 4-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C038888:flavonoid 3'-hydroxylase; D050564:Trans-Cinnamate 4-Monooxygenase; D010650:Phenylalanine Ammonia-Lyase