title: Silencing of 14-3-3ζ over-expression in hepatocellular carcinoma inhibits tumor growth and enhances chemosensitivity to cis-diammined dichloridoplatium.
abstract: The 14-3-3ζ protein plays a key role in regulation of cellular processes. In the present study, we showed that 14-3-3ζ protein was significantly overexpressed in hepatoma cell lines and human tumorous tissues of hepatocellular carcinoma (HCC) patients. Knockdown with RNA interference in hepatoma cell lines with high 14-3-3ζ expression suppressed tumor cell proliferation via activation of c-Jun N-terminal kinase (JNK) and p38/MAPK. Furthermore, suppression of 14-3-3ζ enhanced the anti-cancer effect of cis-diammined dichloridoplatium (CDDP) in hepatoma cell lines. These results suggest that silencing of 14-3-3ζ may be an attractive target for HCC therapeutic development.
authors.affiliation: WHO Collaborating Center of Viral Hepatitis, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Choi
authors.forename: Wonhee
authors.initials: W
authors.lastname: Hur
authors.forename: Chan Kwon
authors.initials: CK
authors.lastname: Jung
authors.forename: Lian Shu
authors.initials: LS
authors.lastname: Piao
authors.forename: Kwangsoo
authors.initials: K
authors.lastname: Lyoo
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Hong
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Yoon
authors.forename: Seung Kew
authors.initials: SK
authors.lastname: Yoon
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics*; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D005434:Flow Cytometry; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D048051:p38 Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D002945:Cisplatin