title: Vascular endothelial growth factor (VEGF) gene polymorphisms and gastric cancer risk in a Chinese Han population.
abstract: The association between vascular endothelial growth factor (VEGF) gene polymorphisms and gastric cancer risk is still controversial and ambiguous. The objective of this study was to investigate the association between VEGF gene polymorphisms and gastric cancer risk in Chinese Han patients. We extracted the peripheral blood samples in 150 patients with gastric cancer and 150 controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was performed to detect three VEGF gene polymorphisms (-634 G/C, +936 C/T, and +1612 G/A) in these patients. Patients with gastric cancer had a significantly higher frequency of 1612 AA genotype (OR=6.26, 95% CI=1.80, 21.85; P=0.004) than controls. Patients with cardia gastric cancer had a significantly lower frequency of AA (OR=0.11, 95% CI=0.01, 0.89; P=0.04) than those with noncardia gastric cancer. Patients with Lauren's diffuse-type gastric cancer had a significantly higher frequency of AA (OR=3.41, 95% CI=1.22, 9.55; P=0.02) than those with Lauren's intestinal-type gastric cancer. The -634 G/C and +936 C/T gene polymorphisms were not associated with a risk of GC and its progression. This study suggests that the VEGF +1612 G/A gene polymorphisms may be associated with gastric cancer in Chinese Han patients, and that difference in genotype distribution may be associated with the location and Lauren's classification of gastric cancer.
authors.affiliation: Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ni
authors.initials: N
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Zhuang
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wu
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A