title: Measurement of phospholipase C by monitoring inositol phosphates using [³H]inositol-labeling protocols in permeabilized cells.
abstract: Hormones, neurotransmitters, chemoattractants, and growth factors all elicit intracellular responses on binding to cell surface receptors by activating inositol phospholipid-specific phospholipase C (PLC). Activated PLC catalyzes the hydrolysis of phosphatidylinositol bisphosphate (PIP(2)), a minor membrane phospholipid, to form two second messengers, diacylglycerol (DAG) and inositol (1,4,5)trisphosphate [Ins(1,4,5,)P(3)]. DAG is a direct activator of protein kinase C isozymes, and Ins(1,4,5)P(3) mobilizes intracellular Ca(2+). G protein-coupled receptors couple to the PLC-β family via G proteins, and tyrosine kinase receptors activate PLC-γ isozymes. Regardless of the PLC isozyme activated, the product is invariantly Ins(1,4,5)P(3).
authors.affiliation: Department of Physiology, University College London, London, UK.
authors.forename: Alison
authors.initials: A
authors.lastname: Skippen
authors.forename: Philip
authors.initials: P
authors.lastname: Swigart
authors.forename: Shamshad
authors.initials: S
authors.lastname: Cockcroft
mesh_terms: D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology*; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D007295:Inositol Phosphates; D014316:Tritium; D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D010738:Type C Phospholipases; D002118:Calcium