title: Conventional and first derivative synchronous fluorometric determination of ethamsylate in pharmaceutical preparations and biological fluids. Application to stability studies.
abstract: Two simple, accurate and highly sensitive spectrofluorometric methods were developed for the determination of ethamsylate (ETM). Method I is based on measuring the native fluorescence of ethamsylate in water at 354 nm after excitation at 302 nm. The calibration plot was rectilinear over the range of 0.05-1 μg/mL for ETM with limits of detection and quantitation of 7.9 and 26 ng/mL, respectively. Method II involved synchronous and first derivative synchronous fluorometric methods for the simultaneous determination of ethamsylate (ETM) and hydroquinone (HQ) which is considered as an impurity and/or acidic degradation product. The synchronous fluorescence of both the drug and its impurity were measured in methanol at Δ λ of 40 nm. The peak amplitudes ((1)D) were estimated at 293.85 or 334.17 nm for ETM and at 309.05 nm for HQ. Good linearity was obtained for ETM over the ranges 0.1-1.4 μg/mL and 0.1-1.0 μg/mL at 293.85 and 334.17 nm, respectively. For HQ, the calibration plot was rectilinear over the range of 0.01-0.14 μg/mL at 309.05 nm. Limits of detection were 20, 2.01 ng/mL and limits of quantitation were 60, 6.7 ng/mL for ETM and HQ by method II, respectively. Both methods were successfully applied to commercial ampoules and tablets. The results were in good agreement with those obtained by the reference method. Method I was utilized to study the stability of ETM and its degradation kinetics using peroxide. The apparent first-order rate constant, half-life times and activation energy of the degradation process were calculated. Method I was further extended to the in-vitro and in-vivo determination of ETM in spiked and real plasma samples. The mean% recoveries were 99.57 ± 3.85 and 89.39 ± 5.93 for spiked and real human plasma, respectively.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.
authors.forename: Fathalla
authors.initials: F
authors.lastname: Belal
authors.forename: Amina
authors.initials: A
authors.lastname: El-Brashy
authors.forename: Nahed
authors.initials: N
authors.lastname: El-Enany
authors.forename: Manar
authors.initials: M
authors.lastname: Tolba
mesh_terms: D000328:Adult; D001826:Body Fluids / Q000737:chemistry*; D004355:Drug Stability; D004979:Ethamsylate / Q000032:analysis*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations; D013501:Surface-Active Agents; D004979:Ethamsylate