title: Roles of inflammatory and anabolic cytokines in cartilage metabolism: signals and multiple effectors converge upon MMP-13 regulation in osteoarthritis.
abstract: Human cartilage is a complex tissue of matrix proteins that vary in amount and orientation from superficial to deep layers and from loaded to unloaded zones. A major challenge to efforts to repair cartilage by stem cell-based and other tissue engineering strategies is the inability of the resident chondrocytes to lay down new matrix with the same structural and resilient properties that it had upon its original formation. This is particularly true of the collagen network, which is susceptible to cleavage once proteoglycans are depleted. Thus, a thorough understanding of the similarities and particularly the marked differences in mechanisms of cartilage remodeling during development, osteoarthritis, and aging may lead to more effective strategies for preventing cartilage damage and promoting repair. To identify and characterize effectors or regulators of cartilage remodeling in these processes, we are using culture models of primary human and mouse chondrocytes and cell lines and mouse genetic models to manipulate gene expression programs leading to matrix remodeling and subsequent chondrocyte hypertrophic differentiation, pivotal processes which both go astray in OA disease. Matrix metalloproteinases (MMP)-13, the major type II collagen-degrading collagenase, is regulated by stress-, inflammation-, and differentiation-induced signals that not only contribute to irreversible joint damage (progression) in OA, but importantly, also to the initiation/onset phase, wherein chondrocytes in articular cartilage leave their natural growth- and differentiation-arrested state. Our work points to common mediators of these processes in human OA cartilage and in early through late stages of OA in surgical and genetic mouse models.
authors.affiliation: Tissue Engineering, Regeneration, and Repair Program, Research Division, The Hospital for Special Surgery,Weill Cornell Medical College, New York, NY 10021, USA. goldringm@hss.edu
authors.forename: Mary B
authors.initials: MB
authors.lastname: Goldring
authors.forename: Miguel
authors.initials: M
authors.lastname: Otero
authors.forename: Darren A
authors.initials: DA
authors.lastname: Plumb
authors.forename: Cecilia
authors.initials: C
authors.lastname: Dragomir
authors.forename: Marta
authors.initials: M
authors.lastname: Favero
authors.forename: Karim
authors.initials: K
authors.lastname: El Hachem
authors.forename: Ko
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Helmtrud I
authors.initials: HI
authors.lastname: Roach
authors.forename: Eleonora
authors.initials: E
authors.lastname: Olivotto
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Borzì
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Marcu
mesh_terms: D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D005109:Extracellular Matrix / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010003:Osteoarthritis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053509:Matrix Metalloproteinase 13