title: Prognostic significance of expression patterns of EGFR family, p21 and p27 in high-grade astrocytoma.
abstract: The goal of this study was to investigate the relationship among immunohistochemical expression of epithelial growth factor receptor (EGFR) family proteins, p21, p27 and prognosis in patients with high-grade astrocytoma. Expression of EGFR family proteins (c-erbB-1, c-erbB-2, c-erbB-3, c-erbB-4), p21 and p27 and Ki-67 labeling index (LI) were studied in 59 samples of high-grade astrocytoma. Expression of protein levels was analyzed by immunohistochemical staining of formalin-fixed and paraffin-embedded sections. Results were analyzed in relation to age, gender and survival. Overexpression of c-erbB-1, c-erbB-2, c-erbB-3 and c-erbB-4 was found in 40 (67.8%), 17 (28.8%), 3 (5.1%) and 42 (75.0%) samples, respectively. Similarly, low expression of p21 and p27 was observed in 50 (84.8%) and 27 (45.8%) samples. Mean Ki-67 LI was 17.3 +/- 1.1. Cox multiple regression analysis showed that c-erbB-1 (Hazard rate(HR) 1.57, 95% Confidence interval (CI) 1.08-2.36; p = 0.017), c-erbB-4 (HR 1.79, 95% CI 1.20-2.74; p = 0.004) and p27 (HR 0.50, 95% CI 0.30-0.82; p = 0.006) were significantly associated with survival. High expression of c-erbB-1 and c-erbB-4 and low expression of p27 were associated with poor prognosis in these patients.
authors.affiliation: Department of Neurosurgery, Hiroshima Prefectural Hospital, Hiroshima 734-8530, Japan. snabika@go4.enjoy.ne.jp
authors.forename: Shinya
authors.initials: S
authors.lastname: Nabika
authors.forename: Katsuzo
authors.initials: K
authors.lastname: Kiya
authors.forename: Hideki
authors.initials: H
authors.lastname: Satoh
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Mizoue
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kondo
authors.forename: Masaya
authors.initials: M
authors.lastname: Katagiri
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishisaka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kurisu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001254:Astrocytoma / Q000737:chemistry / Q000401:mortality*; D001943:Breast Neoplasms / Q000737:chemistry / Q000401:mortality*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000032:analysis*; D050760:Cyclin-Dependent Kinase Inhibitor p27; D066246:ErbB Receptors / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D066247:Receptor, ErbB-4
chemical_list: C493956:CDKN1A protein, human; C436291:CDKN1B protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D047908:Intracellular Signaling Peptides and Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; C587689:ERBB4 protein, human; D066246:ErbB Receptors; D066247:Receptor, ErbB-4