title: Dynamic modeling of lactic acid fermentation metabolism with Lactococcus lactis.
abstract: A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/ MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).
authors.affiliation: Department of Chemial and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Euhlim
authors.initials: E
authors.lastname: Oh
authors.forename: Mingshou
authors.initials: M
authors.lastname: Lu
authors.forename: Changhun
authors.initials: C
authors.lastname: Park
authors.forename: Changhun
authors.initials: C
authors.lastname: Park
authors.forename: Han Bin
authors.initials: HB
authors.lastname: Oh
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D002853:Chromatography, Liquid; D003198:Computer Simulation; D005285:Fermentation; D005635:Fructosediphosphates / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D013294:Lactococcus lactis / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D005635:Fructosediphosphates; D009004:Monosaccharide Transport Proteins; D019344:Lactic Acid; D019298:Glucose-6-Phosphate; D010728:Phosphoenolpyruvate; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; C029063:fructose-1,6-diphosphate