title: Acyldepsipeptide HDAC inhibitor production induced in Burkholderia thailandensis.
abstract: Natural product gene clusters are often tightly regulated, resulting in gene cluster silencing in laboratory fermentation studies. The systematic overexpression of transcription factors (TFs) associated with biosynthetic gene clusters found in the genome of Burkholderia thailandensis E264 identified a set of TFs that, when overexpressed, alter the secondary metabolome of this bacterium. The isolation and characterization of burkholdacs A and B, two new acyldepsitripeptide histone deacetylase inhibitors produced by B. thailandensis overexpressing the TF bhcM, is reported.
authors.affiliation: Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, New York 10065, United States.
authors.forename: John B
authors.initials: JB
authors.lastname: Biggins
authors.forename: Conrad D
authors.initials: CD
authors.lastname: Gleber
authors.forename: Sean F
authors.initials: SF
authors.lastname: Brady
mesh_terms: D019117:Burkholderia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056572:Histone Deacetylase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D005810:Multigene Family; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry; D014157:Transcription Factors / Q000235:genetics
chemical_list: D047630:Depsipeptides; D056572:Histone Deacetylase Inhibitors; D010456:Peptides, Cyclic; D014157:Transcription Factors; C558622:burkholdac A; C558623:burkholdac B; C529984:spiruchostatin B; C485419:spiruchostatin A