title: Measurement of [³H]PN200-110 and [¹²⁵I]ω-conotoxin MVIIA binding.
abstract: Intracellular Ca(2+) ([Ca(2+)](i)) can rise primarily via release from intracellular storage sites (e.g., the endoplasmic reticulum) or via entry across the membrane down the steep concentration gradient. Ca(2+) can enter through two main classes of plasma membrane-located Ca(2+) channels: receptor operated and voltage sensitive. Voltage-sensitive Ca(2+) channels are involved in a wide and diverse array of physiological responses including neurotransmitter release. In addition, many guanine nucleotide protein (G) coupled receptors couple to voltage-sensitive Ca2(+) channels to reduce Ca(2+) influx.
authors.affiliation: Department of Anesthesiology, University of Hirosaki School of Medicine, Hirosaki, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hirota
authors.forename: David G
authors.initials: DG
authors.lastname: Lambert
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002121:Calcium Channel Blockers / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000378:metabolism*; D020864:Calcium Channels, N-Type / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism*; D017275:Isradipine / Q000378:metabolism* / Q000494:pharmacology; D009447:Neuroblastoma; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium / Q000378:metabolism*; D020960:omega-Conotoxins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D020864:Calcium Channels, N-Type; D007457:Iodine Radioisotopes; D020960:omega-Conotoxins; D014316:Tritium; C078452:ziconotide; D017275:Isradipine