title: Changes of androgen receptor and insulin-like growth factor-1 in LNCaP prostate cancer cells treated with sex hormones and flutamide.
abstract: Changes of androgen receptor (AR) and insulin-like growth factor-1 (IGF-1) were investigated in LNCaP cells treated with 5α-dihydrotestosterone (DHT), estrone and flutamide. Real-time PCR, immunocytochemistry and western blotting were used to detect the expression of AR and IGF-1 in the presence or absence of various kinase inhibitors. Low concentrations of DHT, estrone and flutamide increased the expression of AR and IGF-1, especially estrone, with concentration and time dependence. With DHT and flutamide, there was a significant alteration in AR expression (p<0.001). The results indicated expression of AR and IGF-1 genes to be influenced by DHT, estrone and flutamide in LNCaP cells, regulated by multiple signal pathways.
authors.affiliation: Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, China.
authors.forename: Da Yue
authors.initials: da Y
authors.lastname: Tong
authors.forename: Xing Qiao
authors.initials: XQ
authors.lastname: Wen
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.forename: Zhuo Wei
authors.initials: ZW
authors.lastname: Liu
authors.forename: Hong Yu
authors.initials: HY
authors.lastname: Sun
authors.forename: Fang Jian
authors.initials: FJ
authors.lastname: Zhou
authors.forename: Xin Yao
authors.initials: XY
authors.lastname: Wu
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology; D000728:Androgens / Q000494:pharmacology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology; D004970:Estrone / Q000494:pharmacology*; D005485:Flutamide / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: C410798:AR protein, human; D000726:Androgen Antagonists; D000728:Androgens; D004967:Estrogens; D012333:RNA, Messenger; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D004970:Estrone; D007334:Insulin-Like Growth Factor I; D005485:Flutamide