title: Advantages of hyaluronic acid as a component of fibrin sheet for care of acute wound.
abstract: Skin injury is followed by accumulation of a fibrin based provisional matrix which normally drives the process of wound repair. Exogenous fibrin with extra cellular matrix (ECM) components can also favor the wound healing process. In a preliminary study we found that lyophilized fibrin sheet (FS) arrest bleeding from rabbit skin wound but it remained dry during the repair period and did not accelerate the healing process better than untreated control. In the current study, hyaluronic acid (HA) was incorporated into FS and the resultant HA-FS promoted water retention and improved wound healing process. Gross-morphology, histopathology and histomorphometry were employed to compare qualitative and quantitative difference of wound healing in treated group against controls. In experimental sites (HA-FS), re-epithelialization was completed by 14 days with neo-vascularization and deposition of wavy bundles of collagen in the treated sites. Rate of healing process was different in treated and untreated wounds and most striking difference was the appearance of appendages, sebaceous gland and hair follicle at some locations in HA-FS treated sites. Therefore, HA with fibrin can create an effective wound care matrix which promotes water retention and wound healing potential.
authors.affiliation: Laboratory for Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, Kerala, India.
authors.forename: T V
authors.initials: TV
authors.lastname: Anilkumar
authors.forename: Jaseer
authors.initials: J
authors.lastname: Muhamed
authors.forename: Anumol
authors.initials: A
authors.lastname: Jose
authors.forename: Arun
authors.initials: A
authors.lastname: Jyothi
authors.forename: P V
authors.initials: PV
authors.lastname: Mohanan
authors.forename: Lissy K
authors.initials: LK
authors.lastname: Krishnan
mesh_terms: D000208:Acute Disease; D000818:Animals; D005109:Extracellular Matrix / Q000737:chemistry*; D005337:Fibrin / Q000737:chemistry* / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011817:Rabbits; D012867:Skin / Q000098:blood supply / Q000187:drug effects / Q000293:injuries; D017768:Skin, Artificial; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D005337:Fibrin; D006820:Hyaluronic Acid