title: Isolation and characterization of tyramine-producing Enterococcus faecium strains from red wine.
abstract: Enterococcus faecium strains were isolated from red wines undergoing malolactic fermentation and identified by comparison of their 16S rDNA gene sequences with those included in the GenEMBL Databases. The tyrosine decarboxylase gene was identified in all the strains analysed by PCR using gene-specific primers and the ability to produce tyramine in a synthetic media was analysed by RP-HPLC. Survival of an E. faecium strain was also evaluated in microvinification assays using two different musts with different ethanol concentrations (10% and 12% (v/v)). Tyramine production was monitored during the vinification trials. Our results suggest that E. faecium strains isolated from wine are able to produce tyramine and tolerate wine conditions following a pre-acidic stress.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Università di Foggia, via Napoli 25, 71100 Foggia, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Capozzi
authors.forename: Victor
authors.initials: V
authors.lastname: Ladero
authors.forename: Luciano
authors.initials: L
authors.lastname: Beneduce
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
authors.forename: Bach
authors.initials: B
authors.lastname: Benoit
authors.forename: Barnavon
authors.initials: B
authors.lastname: Laurent
authors.forename: Francesco
authors.initials: F
authors.lastname: Grieco
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spano
mesh_terms: D015373:Bacterial Typing Techniques; D004275:DNA, Ribosomal / Q000032:analysis; D016984:Enterococcus faecium / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D006801:Humans; D012336:RNA, Ribosomal, 16S; D014439:Tyramine / Q000096:biosynthesis*; D014445:Tyrosine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D014920:Wine / Q000382:microbiology*
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014445:Tyrosine Decarboxylase; D014439:Tyramine