title: [Simultaneous determination of plantainoside D and verbascoside from stem of Chirita longgangensis var. hongyao by RP-HPLC].
abstract: OBJECTIVE
  To establish a RP-HPLC method for simultaneous determination of phenylethanoid glycosides plantainoside D and verbascoside in Chirita longgangensis var. hongyao.
  
  
  METHODS
  The analysis was performed on a Agilent C18 column (4.6 mm x 250 mm, 5 microm) with CH3CN-1% HAc (16:84)as mobile phase at a flow rate of 1.0 mL x min(-1), and at a column temperature of 30 degrees C. The detection wave length was 332 nm.
  
  
  RESULTS
  The linear ranges of calibration of plantainoside D and verbascoside were 3.125-100.00 mg x L(-1) (r = 0.9998) and 25.00-500.0 mg x L(-1) (r = 0.9998). The average recoveries were 101.3% and 100.8% with RSD of 2.6% and 2.2% (n=9), respectively.
  
  
  CONCLUSIONS
  The method is simple, accurate, reliable and can be used for the quality evaluation of C. longgangensis var. hongyao and its preparation.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
authors.forename: Manyuan
authors.initials: M
authors.lastname: Wang
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Fan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Muxin
authors.initials: M
authors.lastname: Gong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D019684:Magnoliopsida / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry; D059808:Polyphenols
chemical_list: D003373:Coumaric Acids; D004187:Disaccharides; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C516918:plantainoside D; C058956:acteoside; D059808:Polyphenols