title: Propagation, storage, and assays with Hyaloperonospora arabidopsidis: A model oomycete pathogen of Arabidopsis.
abstract: The oomycete pathogen Hyaloperonospora arabidopsidis is a natural pathogen of Arabidopsis thaliana and a laboratory model for (1) understanding how Arabidopsis responds to pathogen attack; (2) comparative and functional genomics of oomycetes; and (3) the molecular basis and evolution of obligate biotrophy. Here, we describe procedures for propagation and long-term storage of H. arabidopsidis, which address complications arising from its biotrophic lifestyle that precludes growth on synthetic media. We also describe four assays that provide information on different facets of the H. arabidopsidis-Arabidopsis interaction.
authors.affiliation: Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA, USA. johnmcd@vt.edu
authors.forename: John M
authors.initials: JM
authors.lastname: McDowell
authors.forename: Troy
authors.initials: T
authors.lastname: Hoff
authors.forename: Ryan G
authors.initials: RG
authors.lastname: Anderson
authors.forename: Daniel
authors.initials: D
authors.lastname: Deegan
mesh_terms: D017360:Arabidopsis / Q000469:parasitology*; D001681:Biological Assay / Q000379:methods*; D046508:Culture Techniques; D054884:Host-Pathogen Interactions; D009868:Oomycetes / Q000166:cytology / Q000472:pathogenicity* / Q000502:physiology*