title: Design and construction of double-decker tile as a route to three-dimensional periodic assembly of DNA.
abstract: DNA is a useful material for nanoscale construction. Due to highly specific Watson-Crick base pairing, the DNA sequences can be designed to form small tiles or origami. Adjacent helices in such nanostructures are connected via Holliday junction-like crossovers. DNA tiles can have sticky ends which can then be programmed to form large one-dimensional and two-dimensional periodic lattices. Recently, a three-dimensional DNA lattice has also been constructed. Here we report the design and construction of a novel DNA cross tile, called the double-decker tile. Its arms are symmetric and have four double helices each. Using its sticky ends, large two-dimensional square lattices have been constructed which are on the order of tens of micrometers. Furthermore, it is proposed that the sticky ends of the double-decker tile can be programmed to form a three-dimensional periodic lattice with large cavities that could be used as a scaffold for precise positioning of molecules in space.
authors.affiliation: Department of Computer Science, Duke University, Durham, North Carolina 27708, United States.
authors.forename: Urmi
authors.initials: U
authors.lastname: Majumder
authors.forename: Abhijit
authors.initials: A
authors.lastname: Rangnekar
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
authors.forename: John H
authors.initials: JH
authors.lastname: Reif
authors.forename: Thomas H
authors.initials: TH
authors.lastname: LaBean
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA