title: Improvement of prostate cancer detection by integrating the PSA test with miRNA expression profiling.
abstract: Prostate-specific antigen (PSA) test is limited in prostate cancer diagnosis due to its inaccuracy. A new approach which integrates the PSA test with miRNA profiling was investigated to improve prostate cancer diagnosis. Six prostate cancer-related miRNAs (miR-16, -21, -34c, -101, -125b, -141) were tested in five cultured prostate cell lines and 20 human prostate specimens. We found that the miRNA expression profiles were significantly different between nontumorigenic and tumorigenic cell lines and specimens. Positive predictive value analysis of prostate cancer was increased from 40% to 87.5% by integrating patient PSA blood levels with miR-21 and miR-141 profiles.
authors.affiliation: Department of Oral Diagnostic Service, College of Dentistry, Howard University, Washington, District of Columbia, USA.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Hao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhao
authors.forename: Chunnian
authors.initials: C
authors.lastname: He
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Pang
authors.forename: T C
authors.initials: TC
authors.lastname: Wu
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Califano
authors.forename: Xinbin
authors.initials: X
authors.lastname: Gu
mesh_terms: D000367:Age Factors; D000368:Aged; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005820:Genetic Testing; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D011237:Predictive Value of Tests; D017430:Prostate-Specific Antigen / Q000097:blood*; D011468:Prostatectomy; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000601:surgery
chemical_list: C533895:MIRN141 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D017430:Prostate-Specific Antigen