title: [Construction and identification of a full-length cDNA library from Spirometra erinaceieuropaei].
abstract: The full-length pBluescript II SK cDNA library of adult Spirometra erinaceieuropaei was constructed by using the SMART method. Data showed that 95.5% of the library was recombinant and the titer of the library was 1.06 x 10(6). The average insert size of the library was about 1.4 kb. Forty-eight randomly selected clones were sequenced. A set of 36 effective expressed sequence tags (ESTs) with the average size of 674 bp was obtained after excluding clones shorter than 450 bp. The unigenes occupied 58.3% of the 36 ESTs. The rate of full-length cDNAs were 57.7% (15/26). The high-quality of full-length cDNA library could be used for large scale EST sequencing.
authors.affiliation: Department of Parasitology, Hainan Medical College, Haikou 571101, China. luganghn@yahoo.com.cn
authors.forename: Gang
authors.initials: G
authors.lastname: Lv
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Lu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Fan
authors.forename: Da-Zhong
authors.initials: DZ
authors.lastname: Shi
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Gan
authors.forename: Sai-Feng
authors.initials: SF
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D013146:Spirometra / Q000235:genetics*
chemical_list: D018076:DNA, Complementary