title: RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice.
abstract: Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 (rlin1), which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen III oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.
authors.affiliation: Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Sun
authors.forename: Linchuan
authors.initials: L
authors.lastname: Liu
authors.forename: Jiuyou
authors.initials: J
authors.lastname: Tang
authors.forename: Aihong
authors.initials: A
authors.lastname: Lin
authors.forename: Fantao
authors.initials: F
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.forename: Genfa
authors.initials: G
authors.lastname: Zhang
authors.forename: Chengcai
authors.initials: C
authors.lastname: Chu
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003304:Coproporphyrinogen Oxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D005808:Genes, Recessive / Q000235:genetics; D005816:Genetic Complementation Test; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D009336:Necrosis / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D012275:Oryza / Q000166:cytology* / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D010641:Phenotype; D045725:Tetrapyrroles / Q000096:biosynthesis
chemical_list: D005819:Genetic Markers; D045725:Tetrapyrroles; D003304:Coproporphyrinogen Oxidase