title: [Cloning and polymorphism analysis of SmERF in Salvia miltiorrhiza].
abstract: The transcription factor of ethylene responsive factor binding protein (ERF) is belonged to AP2/ERF superfamily, which is known to be unique in plants. AP2/ERF proteins have important functions in the transcriptional regulation of a variety of biological processes related to growth and development, as well as various responses to environmental stimuli. An ERF gene from Salvia miltiorrhiza is cloned and divided into ERF gene family group VII of Arabidopsis and Rice. It contains a MCGGAI (I/L) motif referred to as CMVII-1 and a single intron in the 5'-flanking region of the AP2/ERF domain. Sequence analysis reveals that the region of second extron has abundant polymorphism sites. There are 21 single nucleotide polymorphism sites (SNPs) in the 264 bp region, among them, 14 SNPs are synonymous substitutions and 7 SNPs are non-synonymous substitutions. Though analysis of 181 samples from Shandong, Shaanxi and Sichuan Provinces, it reveals that each production area has its own special genotypes, 5 SNPs show significant difference. Cluster based on UPGMA method reveals that different populations from specific province have clustered together. It shows that SmERF gene will be a candidate molecular marker for the identification of Salvia miltiorrhiza from different areas.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Guang-hong
authors.initials: GH
authors.lastname: Cui
authors.forename: Hua
authors.initials: H
authors.lastname: Feng
authors.forename: Wen-yuan
authors.initials: WY
authors.lastname: Li
authors.forename: Wan-yi
authors.initials: WY
authors.lastname: Wang
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Huang
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005787:Gene Frequency; D005838:Genotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D027561:Salvia miltiorrhiza / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; C093680:ethylene-responsive element binding protein