title: [Effect of method of soothing liver and regulating qi on expression of gastrin and somatostatin in hypothalamus, castric antrum of functional dyspepsia rats].
abstract: OBJECTIVE
  To explore the effects of the method of soothing the liver and regulating qi on expression of gastrin and somatostatin in hypothalamus and gastric antrum of functional dyspepsia model rats.
  
  
  METHODS
  The 32 rats were randomly divided into normal group, model group, Chaihu Shugansan group and domperidone group (n = 8). The functional dyspepsia model was established by constantly squeezing their tails and mean while saline, Chaihu Shugansan decoction and domperidone suspension were administered respectively to 4 groups by gavage. The expression of gastrin and somatostatin in hypothalamus and gastric antrum of rats by immunohistochemical were detected 3 weeks later.
  
  
  RESULTS
  The expression of GAS in the hypothalamus and gastric antrum of model group were less than those of normal group (P < 0.05, P < 0.01), while the expression of SS in the hypothalamus and gastric antrum in Model group were significantly increased than those of normal group (P < 0.01). The expression of GAS and SS in gastric antrum of Chaihu Shugansan group and domperidone group were increased and decreased respectively, and the differences were significant (P < 0.05, P < 0.01). There were no obvious difference about expression of GAS, SS in the hypothalamus between domperidone group and model group. GAS expression in hypothalamus of Chaihu Shugansan group were increased than those of normal group but there was no obvious difference in SS expression in hypothalamus between Chaihu Shugansan group and model group.
  
  
  CONCLUSIONS
  The method of soothing the liver and regulating qi can increase GAS expression in central and peripheral and decrease SS expression in peripheral gastric antrum, which may be one of its therapeutic mechanisms on functional dyspepsia.
authors.affiliation: First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. ling_jianghong@sina.com
authors.forename: Jianghong
authors.initials: J
authors.lastname: Ling
authors.forename: Lianming
authors.initials: L
authors.lastname: Wei
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Liang
authors.forename: Daogang
authors.initials: D
authors.lastname: Wang
authors.forename: Yaoli
authors.initials: Y
authors.lastname: Liu
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004415:Dyspepsia / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D005260:Female; D005755:Gastrins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000503:physiopathology*; D011706:Pyloric Antrum / Q000187:drug effects / Q000378:metabolism*; D019413:Qi; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D005755:Gastrins; D013004:Somatostatin