title: Analysis of gene expression in a human-derived glial cell line exposed to 2.45 GHz continuous radiofrequency electromagnetic fields.
abstract: The increasing use of mobile phones has aroused public concern regarding the potential health risks of radiofrequency (RF) fields. We investigated the effects of exposure to RF fields (2.45 GHz, continuous wave) at specific absorption rate (SAR) of 1, 5, and 10 W/kg for 1, 4, and 24 h on gene expression in a normal human glial cell line, SVGp12, using DNA microarray. Microarray analysis revealed 23 assigned gene spots and 5 non-assigned gene spots as prospective altered gene spots. Twenty-two genes out of the 23 assigned gene spots were further analyzed by reverse transcription-polymerase chain reaction to validate the results of microarray, and no significant alterations in gene expression were observed. Under the experimental conditions used in this study, we found no evidence that exposure to RF fields affected gene expression in SVGp12 cells.
authors.affiliation: Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sakurai
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kiyokawa
authors.forename: Eijiro
authors.initials: E
authors.lastname: Narita
authors.forename: Yukihisa
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masao
authors.initials: M
authors.lastname: Taki
authors.forename: Junji
authors.initials: J
authors.lastname: Miyakoshi
mesh_terms: D000042:Absorption; D001253:Astrocytes / Q000378:metabolism; D002460:Cell Line / Q000528:radiation effects*; D017931:DNA Primers / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008872:Microwaves; D009457:Neuroglia / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011846:Radio Waves; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers