title: Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium.
abstract: Coherent Raman microspectroscopy imaging is an emerging technique for noninvasive, chemically specific optical imaging, which can be potentially used to analyze the chemical composition and its distribution in biological tissues. In this report, a hierarchical cluster analysis was applied to hyperspectral coherent anti-Stokes Raman imaging of different chemical species through a turbid medium. It was demonstrated that by using readily available commercial software (Cytospec, Inc.) and cluster analysis, distinct chemical species can be imaged and identified through a rather thick layer of scattering medium. Once the clusters of different chemical composition were distinguished, a phase retrieval algorithm was used to convert coherent anti-Stokes Raman spectra to Raman spectra, which were used for chemical identification of hidden microscopic objects. In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated.
authors.affiliation: University of Wisconsin - Milwaukee, Department of Physics, 1900 East Kenwood Boulevard, Milwaukee, Wisconsin 53211, USA.
authors.forename: Rajan
authors.initials: R
authors.lastname: Arora
authors.forename: Georgi I
authors.initials: GI
authors.lastname: Petrov
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Yakovlev
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*