title: Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology.
abstract: BACKGROUND
  The prostaglandin D2 (PGD2) pathway is involved in numerous biological processes and while it has been identified as a partner of the embryonic sex determining male cascade, the roles it plays in ovarian function remain largely unknown. PGD2 is secreted by two prostaglandin D synthases (Pgds); the male-specific lipocalin (L)-Pgds and the hematopoietic (H)-Pgds.
  
  
  METHODS
  To study the expression of the Pgds in the adult ovary, in situ hybridization were performed. Then, to evaluate the role of H-Pgds produced PGD2 in the ovarian physiology, adult female mice were treated with HQL-79, a specific inhibitor of H-Pgds enzymatic activity. The effects on expression of the gonadotrophin receptors FshR and LhR, steroidogenic genes Cyp11A1, StAR and on circulating progesterone and estradiol, were observed.
  
  
  RESULTS
  We report the localization of H-Pgds mRNA in the granulosa cells from the primary to pre-ovulatory follicles. We provide evidence of the role of H-Pgds-produced PGD2 signaling in the FSH signaling through increased FshR and LhR receptor expression. This leads to the activation of steroidogenic Cyp11A1 and StAR gene expression leading to progesterone secretion, independently on other prostanoid-synthetizing mechanisms. We also identify a role whereby H-Pgds-produced PGD2 is involved in the regulation of follicular growth through inhibition of granulosa cell proliferation in the growing follicles.
  
  
  CONCLUSIONS
  Together, these results show PGD2 signaling to interfere with FSH action within granulosa cells, thus identifying an important and unappreciated role for PGD2 signaling in modulating the balance of proliferation, differentiation and steroidogenic activity of granulosa cells.
authors.affiliation: Institut de Génétique Humaine, Department of Genetic and Development, CNRS UPR1142, 141, rue de la Cardonille, 34396 Montpellier CEDEX5, France. boizet@igh.cnrs.fr.
authors.forename: Andalib
authors.initials: A
authors.lastname: Farhat
authors.forename: Pascal
authors.initials: P
authors.lastname: Philibert
authors.forename: Charles
authors.initials: C
authors.lastname: Sultan
authors.forename: Francis
authors.initials: F
authors.lastname: Poulat
authors.forename: Brigitte
authors.initials: B
authors.lastname: Boizet-Bonhoure