title: Intra-operative washing of morcellised bone allograft with pulse lavage: how effective is it in reducing blood and marrow content?
abstract: The use of unprocessed bone carries a risk of transmission of blood borne diseases. Although models of infectivity are unproven, a theoretical risk of transmission of variant Creutzfeld-Jakob Disease, a human prion disease, exists as probable blood borne transmission has been reported in three cases. The aim of our study was to determine the effectiveness of standard operating theatre pulse lavage in removing protein, fat and double stranded Deoxyribonucleic acid (dsDNA) from morcellised bone allograft. Twelve donated femoral heads were divided into halves and milled into bone chips. One half of the bone chips were washed with pulse lavage, whereas, the other half acted as control. In order to determine the amount of protein, fat and dsDNA present in the washed and unwashed samples, a validated multistep washing protocol was used. Using the validated technique, simple intra-operative washing of morcellised unprocessed bone allograft removed a significant amount of the protein (70.5%, range: 39.5-85%), fat (95.2%, range: 87.8-98.8%) and DNA (68.4%, range: 31.4-93.1%) content. Intra-operative washing of morcellised bone allograft with pulse lavage may thereby reduce the theoretical risk of prion and other blood borne disease transmission. Combined with the known improved mechanical characteristics of washed allograft, we would recommend pulse lavage as a routine part of bone allograft preparation.
authors.affiliation: Department of Orthopaedic Surgery, Glenfield Hospital, Groby Road, Leicester LE3 9QP, UK. ti11@le.ac.uk
authors.forename: T
authors.initials: T
authors.lastname: Ibrahim
authors.forename: A
authors.initials: A
authors.lastname: Qureshi
authors.forename: T A
authors.initials: TA
authors.lastname: McQuillan
authors.forename: J
authors.initials: J
authors.lastname: Thomson
authors.forename: G
authors.initials: G
authors.lastname: Galea
authors.forename: R A
authors.initials: RA
authors.lastname: Power
mesh_terms: D001769:Blood / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism*; D016025:Bone Transplantation / Q000379:methods*; D002498:Centrifugation; D004247:DNA / Q000302:isolation & purification; D005270:Femur Head / Q000637:transplantation; D006801:Humans; D007430:Intraoperative Care / Q000379:methods*; D008055:Lipids / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D012995:Solubility; D007507:Therapeutic Irrigation / Q000379:methods*; D014184:Transplantation, Homologous
chemical_list: D008055:Lipids; D011506:Proteins; D004247:DNA