title: Histochemistry: live and in color.
abstract: Histochemistry-chemistry in the context of biological tissue-is an invaluable set of techniques used to visualize biological structures. This field lies at the interface of organic chemistry, biochemistry, and biology. Integration of these disciplines over the past century has permitted the imaging of cells and tissues using microscopy. Today, by exploiting the unique chemical environments within cells, heterologous expression techniques, and enzymatic activity, histochemical methods can be used to visualize structures in living matter. This review focuses on the labeling techniques and organic fluorophores used in live cells.
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA. lavisl@janelia.hhmi.org
authors.forename: Luke D
authors.initials: LD
authors.lastname: Lavis
mesh_terms: D000818:Animals; D002470:Cell Survival; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005818:Genetic Engineering; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008025:Ligases / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008025:Ligases