title: Two-dimensional gel proteome reference map of INS-1E cells.
abstract: The insulin-producing INS-1E rat cell line is widely used as a model for studying β-cells. It is a well-characterized cell line, mainly used in diabetes research. We established a 2-DE reference map for INS-1E cells. Using MALDI-TOF/TOF-MS/MS, we identified 546 spots. These included various proteins with an important role in β-cell physiology and with known roles as crucial proteins for diabetes development. We believe that the availability of this reference map will enhance our knowledge of β-cell physiology.
authors.affiliation: Laboratory for Experimental Medicine and Endocrinology, Catholic University of Leuven, Leuven, Belgium.
authors.forename: Wannes
authors.initials: W
authors.lastname: D'Hertog
authors.forename: Michael
authors.initials: M
authors.lastname: Maris
authors.forename: Lieven
authors.initials: L
authors.lastname: Thorrez
authors.forename: Etienne
authors.initials: E
authors.lastname: Waelkens
authors.forename: Lut
authors.initials: L
authors.lastname: Overbergh
authors.forename: Chantal
authors.initials: C
authors.lastname: Mathieu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D030562:Databases, Protein; D003920:Diabetes Mellitus / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D007340:Insulinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins; D020543:Proteome