title: A highly efficient and versatile microchip capillary electrophoresis method for DNA separation using gold nanoparticle as a tag.
abstract: A highly efficient and versatile method for DNA separation using Au nanoparticles (Au NPs) as a tag based on microchip capillary electrophoresis (MCE) was developed. The thiol-modified DNA-binding Au NPs were utilized as a tag. Target DNA was sandwiched between Au NPs and probe DNA labeled with horseradish peroxidase (HRP). In electrophoresis separation, the difference in electrophoretic mobility between free probe and probe-target complex was magnified by Au NPs, which enabled the resulting mixture to be separated with high efficiency by microchip capillary electrophoresis. Horseradish peroxidase was used as a catalytic label to achieve sensitive electrochemical DNA detection via fast catalytic reactions. With this protocol, 27-mer DNA fragments with different sequences were separated with high speed and high resolution. The proposed method was critical to achieve improved DNA separations in hybridization analyses.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, P. R. China. cw1968@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Youjian
authors.initials: Y
authors.lastname: Fu
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Tan
authors.forename: Bin
authors.initials: B
authors.lastname: Qu
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D015342:DNA Probes; D006046:Gold; D004247:DNA; D006735:Horseradish Peroxidase