title: [Overexpression of laminine-5( LN-5) in peritoneal lavage of colorectal cancer patients preliminary results].
abstract: Recent studies show that interaction between LN (heterotrimeric protein formed by a3/b3/g2 chains) and cancer cells plays an important role in tumor invasion, also in colorectal cancer. The overall survival was significantly worse in patients with free peritoneal cancer cells(FPTCs): detection of FPTCs after curative surgery is a challenge, because could improve staging and prognosis. Peritoneal citology is the current standard procedure with very low sensivity. We aimed to study the expression of LN5 in the peritoneal lavage of colorectal cancer pts and in controls with semiquantitative reverse trancriptase-polymerase chain reaction (RT-PCR). LN-5 overexpression was evaluated observing PCR- products intensity at electrophoresis: high intensity is correlated to overexpression. Pre and post-operative peritoneal lavages of 30 pts with colorectal cancer (13M;17F), with median age of 69 (58-84), and of 10 controls, were analyzed by conventional cytology and a semiquantitative RT-PCR. No cancer pts showed pre/postoperative negative cytology and did not express LN-5. In cancer pts. cytology was positive in 2 pts in pre/postoperative lavage. LN-5 overexpression was observed in 56,6% preoperatively and in 76,6% postoperatively. LN-5 g 2 chain was most frequent chain. Our study suggests a relationship between LN-5 and FPTCs, as shown by the low expression of lamimine in controls. LN-5 could be a useful marker to identify a subgroup of early-stage patients at increased risk of recurrence; moreover, mortality seems to correlated to LAMB3 chain. The diagnostic accuracy could be improved by using a quantitative RT-PCR or western-blot and detecting serum laminine. Finally, to validate these findings a larger number of pts with follow-up study is required.
authors.affiliation: Dipartimento d'Emergenza e Trapianti d'Organo Unita Operativa di Chirurgia Generale III Universitaria.
authors.forename: A
authors.initials: A
authors.lastname: Volpi
authors.forename: G
authors.initials: G
authors.lastname: D'Elia
authors.forename: O C
authors.initials: OC
authors.lastname: Pannarale
authors.forename: F
authors.initials: F
authors.lastname: Di Gennaro
authors.forename: P
authors.initials: P
authors.lastname: Guida
authors.forename: E
authors.initials: E
authors.lastname: Martinelli
authors.forename: A
authors.initials: A
authors.lastname: Kavvadias
authors.forename: G
authors.initials: G
authors.lastname: Balducci
authors.forename: P
authors.initials: P
authors.lastname: Ialongo
authors.forename: A
authors.initials: A
authors.lastname: Panebianco
authors.forename: M
authors.initials: M
authors.lastname: De Luca
authors.forename: G
authors.initials: G
authors.lastname: Fabiano
authors.forename: N
authors.initials: N
authors.lastname: Palasciano
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000601:surgery; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008875:Middle Aged; D010533:Peritoneal Lavage; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097794:Kalinin
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D007797:Laminin; D000097794:Kalinin