title: Size-dependant cellular uptake of dendritic polyglycerol.
abstract: To study the mechanism of cellular internalization, hyperbranched polyether derivatives consisting of amino-bearing hyperbranched polyglycerols (HPGs) of varied molecular mass and size range are designed and synthesized. HPGs were further fluorescently labelled by conjugating maleimido indocarbocyanine dye (ICC-mal). The conjugates are characterized by UV-vis spectroscopy, fluorescence profile, zeta potential, and dynamic light scattering. The uptake mechanism is studied by fluorescence-activated cell sorting (FACS) analysis, fluorescence spectroscopy, and confocal microscopy with human lung cancer cells A549, human epidermoid carcinoma cells A431, and human umbilical vein endothelial cells (HUVEC) cells. For the first time, the results suggest that the higher-molecular-weight HPGs (40-870 kDa) predominantly accumulate in the cytoplasm much better than their low-molecular-weight counterparts (2-20 kDa). The HPG nanocarriers discussed here have many biomedical implications, particularly for delivering drugs to the targeted site.
authors.affiliation: Department of Biology, Chemistry, and Pharmacy, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Reichert
authors.forename: Pia
authors.initials: P
authors.lastname: Welker
authors.forename: Marcelo
authors.initials: M
authors.lastname: Calderón
authors.forename: Jayant
authors.initials: J
authors.lastname: Khandare
authors.forename: Dorothea
authors.initials: D
authors.lastname: Mangoldt
authors.forename: Kai
authors.initials: K
authors.lastname: Licha
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Kainthan
authors.forename: Donald E
authors.initials: DE
authors.lastname: Brooks
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D004337:Drug Carriers; D011108:Polymers; C043941:polyglycerol; D005990:Glycerol