title: Enhancing the soft tissue seal around intraosseous transcutaneous amputation prostheses using silanized fibronectin titanium alloy.
abstract: The success of intraosseous transcutaneous amputation prostheses (ITAP) relies on achieving a tight seal between the soft tissues and the implant in order to avoid infection. Fibronectin (Fn) may be silanized onto titanium alloy (Ti-6Al-4V) in order to promote soft-tissue attachment. The silanization process includes passivation with sulphuric acid, which alters surface characteristics. This study aimed to improve in vitro fibroblast adhesion to silanized fibronectin (SiFn) titanium alloy by omitting the passivation stage. Additionally, the study assessed the effects of SiFn on in vivo dermal attachment, comparing the results with adsorbed Fn, hydroxyapatite (HA), Fn adsorbed onto HA (HAFn) and uncoated controls. Surface topography was assessed using scanning electron microscopy, profilometry and contact angle measurement. Anti-vinculin antibodies were used to immunolocalize fibroblast adhesion sites. A histological assessment of soft-tissue attachment and cell alignment relative to implants in an in vivo ovine model was performed. Passivation resulted in rougher, more hydrophobic, microcracked surfaces and was associated with poorer fibroblast adhesion than unpassivated controls. SiFn and HAFn surfaces resulted in more favourable cell alignment in vivo, implying that dermal attachment was enhanced. These results suggest that SiFn and HAFn surfaces could be useful in optimizing the soft tissue seal around ITAP.
authors.affiliation: Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, University College London, Brockley Hill, Stanmore, HA7 4LP, UK. mukai.cg@mac.com
authors.forename: M
authors.initials: M
authors.lastname: Chimutengwende-Gordon
authors.forename: C
authors.initials: C
authors.lastname: Pendegrass
authors.forename: G
authors.initials: G
authors.lastname: Blunn
mesh_terms: D000327:Adsorption; D000497:Alloys / Q000737:chemistry*; D000671:Amputation, Surgical / Q000379:methods; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D016348:Osseointegration; D012756:Sheep; D012825:Silicon / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D005353:Fibronectins; D013464:Sulfuric Acids; D017886:Durapatite; D014025:Titanium; C033158:sulfuric acid; D012825:Silicon