title: [Effect of polydatin on dynamic changes of excitatory amino acids in cerebrospinal fluid of cerebral hemorrhage rats].
abstract: OBJECTIVE
  To observe the effects of polydatin on dynamic changes of excitatory amino acids in cerebrospinal fluid and water content of brain tissue of cerebral hemorrhage rats. And to discuss the therapeutic action and mechanisms of polydatin on brain hemorrhagic injured rats.
  
  
  METHODS
  A quantitative determination method of Asp and Glu was established by microdialysis-HPLC. The cerebral hemorrhage model in rats was induced by local injection of type VII collagenase. The dynamic changes of Asp and Glu in cerebrospinal fluid were observed on 0, 6, 12, 24, 36, 48, 60, 72, 84, 96, 108 h of cerebral hemorrhage rats, and then the water content of brain tissue was detected.
  
  
  RESULTS
  The content of Asp and Glu increased rapidly within 24 h after cerebral hemorrhage, and to the highest in 24 h, then decreased gradually. Compared with the cerebral hemorrhage model group, the content of Asp and Glu increased slowly in polydatin group, and there were significant differences in 12-72 h and 6-84 h (P < 0.01, P < 0.05), but there was no significant difference after 84 h and 96 h. Compared with sham group, water content of brain tissue significantly higher in model group, while significantly lower (P < 0.01) in polydatin group.
  
  
  CONCLUSIONS
  Polydatin can inhibit increasing content of Asp and Glu in cerebrospinal fluid dynamics, and significantly inhibit cerebral edema of cerebral hemorrhage rats. It shows that the mechanisms of anti-cerebral hemorrhage injury of polydatin may be related to increasing of excitatory amino acids after cerebral hemorrhage.
authors.affiliation: Second Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310009, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Bie
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Jiehong
authors.initials: J
authors.lastname: Yang
authors.forename: Haitong
authors.initials: H
authors.lastname: Wan
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000134:cerebrospinal fluid; D002543:Cerebral Hemorrhage / Q000134:cerebrospinal fluid / Q000188:drug therapy*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D018846:Excitatory Amino Acids / Q000134:cerebrospinal fluid*; D005960:Glucosides / Q000627:therapeutic use*; D018698:Glutamic Acid / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000627:therapeutic use*
chemical_list: D004365:Drugs, Chinese Herbal; D018846:Excitatory Amino Acids; D005960:Glucosides; D013267:Stilbenes; D001224:Aspartic Acid; D018698:Glutamic Acid; C058229:polydatin