title: Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish.
abstract: The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100pg/ml-10ng/ml) significantly increased ALP activity at 6h of incubation. High-dose (10ng/ml) fugu PTH1 significantly increased ALP activity even after 18h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6h of incubation, but fugu PTH1 (100pg/ml-10ng/ml) significantly increased TRAP activity at 18h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Housu-gun, Ishikawa, Japan. nobuos@staff.kanazawa-u.ac.jp
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
authors.forename: Janine A
authors.initials: JA
authors.lastname: Danks
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Maruyama
authors.forename: Mika
authors.initials: M
authors.lastname: Ikegame
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sasayama
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hattori
authors.forename: Masahisa
authors.initials: M
authors.lastname: Nakamura
authors.forename: Makoto J
authors.initials: MJ
authors.lastname: Tabata
authors.forename: Toshio
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Ryo
authors.initials: R
authors.lastname: Furuya
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Saijoh
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mishima
authors.forename: Ajai K
authors.initials: AK
authors.lastname: Srivastav
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Furusawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takasaki
authors.forename: Vishwajit S
authors.initials: VS
authors.lastname: Chowdhury
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
authors.forename: T John
authors.initials: TJ
authors.lastname: Martin
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000825:Animal Structures / Q000187:drug effects* / Q000201:enzymology / Q000637:transplantation / Q000648:ultrastructure; D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D015726:Giant Cells / Q000166:cytology / Q000187:drug effects; D006054:Goldfish / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000637:transplantation; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D010281:Parathyroid Hormone / Q000494:pharmacology*; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics / Q000378:metabolism; D018016:Receptors, Parathyroid Hormone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D023741:Takifugu; D000071681:Tartrate-Resistant Acid Phosphatase; D014182:Transplantation, Autologous
chemical_list: D007527:Isoenzymes; D010281:Parathyroid Hormone; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; D018016:Receptors, Parathyroid Hormone; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002118:Calcium