title: A study of anti-cancer effects of Funalia trogii in vitro and in vivo.
abstract: Extracts of natural products have been used for many years for health benefits. We report on an in vitro and in vivo study into the anti-tumour efficacy of an aqueous extract of the mycelial form of basidiomycete, Funalia trogii. A variety of biological assays were used to show that a 4h exposure of HT29, LNCaP, PC3, MCF-7 and MDA-MB-231 tumour cells to extract (0.5-5.0 mg/ml) resulted in significant cytotoxicity. In a clonogenic assay, IC50 values were found to range from 0.4-0.72 mg/ml; exposing fibroblast cells to the extract resulted in no cell kill. The extract resulted in significant cell kill in proliferating endothelial cells but had no toxicity to quiescent cells, this is useful in targeting tumour tissue since endothelial cells in tumours proliferate more rapidly that those found in other parts of the body. When tumours grown in immune compromised mice were injected intratumourally with extract (5 mg/ml twice a week for two weeks), a 9 day tumour growth delay was observed. The results indicate that the mycelial extract of F. trogii has a promising anti-tumour property.
authors.affiliation: Biomedical Sciences Research Institute, University of Ulster, Cromore Road, Coleraine, Co-Londonderry BT52 1SA, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Rashid
authors.forename: A
authors.initials: A
authors.lastname: Unyayar
authors.forename: M A
authors.initials: MA
authors.lastname: Mazmanci
authors.forename: S R
authors.initials: SR
authors.lastname: McKeown
authors.forename: I M
authors.initials: IM
authors.lastname: Banat
authors.forename: J
authors.initials: J
authors.lastname: Worthington
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D055454:Trametes / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins