title: Expression of pigment epithelium-derived factor in bladder tumour is correlated with interleukin-8 yet not with interleukin-1α.
abstract: Pigment epithelium-derived factor (PEDF) is an antiangiogenic factor which is effective in tumour inhibition in a variety of tumours and has not yet been studied in bladder tumour before. In this study the expression of PEDF, interleukin-1α (IL-1α) and -8 (IL-8) in bladder tumours was investigated. Immunohistochemistry was performed on 64 bladder tumour and 23 normal uroepithelium samples. Expression change of the factors was compared with clinicopathological parameters. Correlations between PEDF, IL-1α and IL-8 were analyzed. None of the factors was in relation to gender, tumour occurrence, and size or onset pattern. PEDF (P=0.014) and IL-1α (P=0.049) expression was down-regulated with grade progression. PEDF expression was lower in normal uroepithelium than in papillary urothelial neoplasm of low malignant potential (PUNLMP) (P=0.000) and carcinoma (P=0.009) whilst IL-1α (P=0.000 and P=0.000 respectively) and IL-8 (P=0.000 and P=0.023 respectively) expression was higher in the same grouping. PEDF expression had a negative correlation with IL-8 in PUNLMP (P=0.049, r=-0.578) as well as in tumour grouping (P=0.033, r=-0.276). Deranged expressional change of PEDF, IL-1α and IL-8 could be in relation to loss of differentiation from normal uroepithelium to papillary lesion and eventually to carcinoma.
authors.affiliation: Department of Urology, Fudan University, Shanghai, 200040, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Feng
authors.affiliation: Centre of Laboratory Medicine, Fudan University, Shanghai, 200040, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.affiliation: Department of Urology, Fudan University, Shanghai, 200040, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Institute of Urology, Fudan University, Shanghai, 200040, China.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Urology, Fudan University, Shanghai, 200040, China.
authors.forename: Haowen
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Urology, Fudan University, Shanghai, 200040, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.affiliation: Department of Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, China.
authors.forename: Paohsun
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Urology, Fudan University, Shanghai, 200040, China. 09211220046@fudan.edu.cn.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002291:Carcinoma, Papillary / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism*; D055815:Young Adult; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D005136:Eye Proteins; D053582:Interleukin-1alpha; D016209:Interleukin-8; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor