title: Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china.
abstract: In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp fragment of the nucleoprotein (N) gene of CDV. By RFLP analysis using BamHI, field isolates were distinguishable from the vaccine strains. Two fragments were obtained from the vaccine strains by RT-PCR-RFLP analysis while three were observed in the field strains. An 829 nucleotide region of the CDV N gene was analyzed in 19 CDV field strains isolated from minks, raccoon dogs and foxes in China between 2005 and 2007. The results suggest this method is precise, accurate and efficient. It was also determined that three different genotypes exist in CDV field strains in fur animal herds of the north of China, most of which belong to Asian type. Mutated field strains, JSY06-R1, JSY06-R2 and JDH07-F1 also exist in Northern China, but are most closely related to the standard virulent strain A75/17, designated in Arctic and America-2 genetype in the present study, respectively.
authors.affiliation: Jilin Zhongte Biotechnological Co, Ltd, Jilin 132109, People's Republic of China. yanxijungmp@yahoo.com.cn
authors.forename: Fengxue
authors.initials: F
authors.lastname: Wang
authors.forename: Xijun
authors.initials: X
authors.lastname: Yan
authors.forename: Xiuli
authors.initials: X
authors.lastname: Chai
authors.forename: Hailing
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhao
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wen
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D002681:China; D004216:Distemper / Q000175:diagnosis / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs; D005589:Foxes; D008907:Mink; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D046031:Raccoon Dogs; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014765:Viral Vaccines / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D014765:Viral Vaccines