title: Lack of association of single nucleotide polymorphisms of the bovine Flt-1 gene with growth traits in Chinese cattle breeds.
abstract: We analyzed 20 exons, with their intron-exon boundaries, of the bovine Flt-1 gene, using a strategy combining PCR amplification and single-strand conformational polymorphism analysis (PCR-SSCP), followed by nucleotide sequence analysis, in 675 cattle. We then looked for associations between polymorphisms and growth traits. Twelve novel SNPs (ss#184956516, ss#184956517, ss#184956518, ss#184956519, ss#251343993, ss#251343994, ss#251343995, ss#251343996, ss#251343997, ss#251343998, ss#251343999, and ss#251344000) were detected in the bovine Flt-1 gene in all three breeds. We observed no significant associations between these polymorphisms and birth weight, body weight and average daily gain during different growth periods (6, 12, 18, and 24 months old) (P > 0.05), or in body height, body length, heart girth, or height at the hip in Nanyang cattle breeds.
authors.affiliation: College of Animal Science and Technology, Northwest A and F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Pang
authors.forename: C Z
authors.initials: CZ
authors.lastname: Lei
authors.forename: C L
authors.initials: CL
authors.lastname: Zhang
authors.forename: X Y
authors.initials: XY
authors.lastname: Lan
authors.forename: S
authors.initials: S
authors.lastname: Shao
authors.forename: X M
authors.initials: XM
authors.lastname: Gao
authors.forename: J Q
authors.initials: JQ
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics*
chemical_list: D040281:Vascular Endothelial Growth Factor Receptor-1