title: Chikungunya virus isolates with/without A226V mutation show different sensitivity to IFN-a, but similar replication kinetics in non human primate cells.
abstract: Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus responsible for the first autochthonous Italian outbreak in 2007. A226V mutation in E1 has been associated with enhanced replication in A. albopictus vector. Possible involvement of this mutation in enhanced infection capability in primate cells and sensitivity to exogenous interferon (IFN)-a was investigated. No significant differences were observed between the two isolates in terms of replication kinetic, virus yield and cytopathic effect (CPE). Interestingly, the A226V-carrying strain was more susceptible to the antiviral action of recombinant IFN-a. The interplay between A226V mutation and innate defence mechanisms needs further investigation.
authors.affiliation: Laboratory of Virology, National Institute for Infectious Diseases L. Spallanzani, Rome, Italy.
authors.forename: Licia
authors.initials: L
authors.lastname: Bordi
authors.forename: Silvia
authors.initials: S
authors.lastname: Meschi
authors.forename: Marina
authors.initials: M
authors.lastname: Selleri
authors.forename: Eleonora
authors.initials: E
authors.lastname: Lalle
authors.forename: Concetta
authors.initials: C
authors.lastname: Castilletti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Carletti
authors.forename: Roberta
authors.initials: R
authors.lastname: Chiappini
authors.forename: Antonino
authors.initials: A
authors.lastname: Di Caro
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Capobianchi
mesh_terms: D018354:Alphavirus Infections / Q000276:immunology / Q000821:virology*; D000818:Animals; D002646:Chikungunya virus / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D002522:Chlorocebus aethiops; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000494:pharmacology*; D020125:Mutation, Missense; D011994:Recombinant Proteins; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014779:Virus Replication / Q000235:genetics
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins