title: Extended haplotype analysis of ovine ADRB3 using polymerase chain reaction single strand conformational polymorphism on two regions of the gene.
abstract: The β3 adrenergic receptor (ADRB3) plays a critical role in the regulation of energy metabolism in mammals. In sheep, intronic polymorphism of the ADRB3 gene has been associated with lamb survival and various production traits. This study investigates variation in the ovine ADRB3 3' untranslated region (3'UTR), a region that may impact expression of the gene. Using PCR- single strand conformational polymorphism (SSCP), six unique patterns (named a-f) were observed in an approximately 304-bp amplicon. Sequencing revealed three single-nucleotide polymorphisms (c.*233A>C, c.*271G>C, c.*357A>T) and a single-nucleotide deletion (c.*257delG). Haplotype analyses showed that the previously described allele A defined by variation in the ovine ADRB3 intron can be divided into three haplotypes (Aa, Ab, and Ac). In total, 16 haplotypes through ovine ADRB3 were detected. This study suggests that ovine ADRB3 is highly polymorphic and that the extended haplotype analysis through the promoter, 5'UTR, coding sequence, intron, and 3'UTR needs to be performed to define the full extent of variation in this gene.
authors.affiliation: Faculty of Agriculture and Life Science, Lincoln University, Lincoln, New Zealand.
authors.forename: Guo
authors.initials: G
authors.lastname: Yang
authors.forename: Jon G H
authors.initials: JG
authors.lastname: Hickford
authors.forename: Huitong
authors.initials: H
authors.lastname: Zhou
authors.forename: Qian
authors.initials: Q
authors.lastname: Fang
authors.forename: Rachel H
authors.initials: RH
authors.lastname: Forrest
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D006239:Haplotypes; D007438:Introns; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D012756:Sheep / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D022702:Receptors, Adrenergic, beta-3