title: Quantitative determination of 10 phenylpropanoid and lignan compounds in Lancea tibetica by high-performance liquid chromatography with UV detection.
abstract: An HPLC method was developed for simultaneous determination of one phenylpropanoid glycoside, verbascoside (1), and nine lignans, including lantibeside (2), phillyrin (3), lantibeside B (4), lantibeside C (5), tibeticoside A (6), styraxjaponoside C (7), sylvatesmin (8), (+)-piperitol (9), and horsfieldin (10), from the Tibetan medicinal plant Lancea tibetica Hook. F. et Thoms. The analysis was performed within 45 min. The extraction method was optimized with different solvent systems. The HPLC method was validated for linearity, repeatability, accuracy, limits of detection, and limits of quantification. The limits of detection and limits of quantification of 10 analytes were found to be less than 0.1 and 0.5 µg/mL, respectively. The RSD for intra- and inter-day analyses was less than 4.2 %, and the recovery efficiency was 90-105 %. The method was used to analyze different populations of L. tibetica collected in China. HPLC profiles showed that the concentrations of analytes were different in samples collected from different areas of China. Verbascoside was the dominant component in three out of five plant samples; compounds 2, 3, 6, and 8 accounted for over 62 % yields in total lignan contents. The method is useful for identification, quality assurance, and quality control of L. tibetica and its related products.
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA.
authors.forename: Zong-Hua
authors.initials: ZH
authors.lastname: Song
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Zhong-Zhi
authors.initials: ZZ
authors.lastname: Qian
authors.forename: Troy J
authors.initials: TJ
authors.lastname: Smillie
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D002138:Calibration; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D038861:Medicine, Tibetan Traditional; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D011786:Quality Control; D028083:Scrophulariaceae / Q000737:chemistry*; D014466:Ultraviolet Rays; D059808:Polyphenols
chemical_list: D005960:Glucosides; D017705:Lignans; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols