title: Inhibition of Candida albicans biofilm formation and yeast-hyphal transition by 4-hydroxycordoin.
abstract: Candida albicans distinguishing features such as dimorphism and biofilm formation are thought to play a key role in oral tissue invasion and resistance to host defences and antifungal agents. In this study, we investigated the effect of 4-hydroxycordoin, a natural isopentenyloxychalcone, on growth, biofilm formation and yeast-hyphal transition of C. albicans. Serial dilutions of 4-hydroxycordoin in YNB medium were prepared in microplates to determine minimal inhibitory concentrations (MIC) and effects on biofilm formation for two strains of C. albicans. 4-Hydroxycordoin at up to 200 μg/ml had no effect on growth of C. albicans. Biofilm formation was strongly inhibited (>85%) by 4-hydroxycordoin at 20 μg/ml, while concentrations ranging from 50 to 200 μg/ml caused a significant inhibition of yeast-hyphal transition, as determined by microscopic observation. In conclusion, 4-hydroxycordoin exerts inhibitory effects on two important virulence factors of C. albicans: biofilm formation or yeast-hyphal transition. This suggests that 4-hydroxycordoin may have a therapeutic potential for C. albicans infections.
authors.affiliation: Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Céline
authors.initials: C
authors.lastname: Messier
authors.forename: Francesco
authors.initials: F
authors.lastname: Epifano
authors.forename: Salvatore
authors.initials: S
authors.lastname: Genovese
authors.forename: Daniel
authors.initials: D
authors.lastname: Grenier
mesh_terms: D000935:Antifungal Agents / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology; D047188:Chalcones / Q000008:administration & dosage / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D014774:Virulence / Q000187:drug effects
chemical_list: C558040:4-hydroxycordoin; D000935:Antifungal Agents; D047188:Chalcones