title: HPV L1 capsid protein detection and progression of anal squamous neoplasia.
abstract: The progression of cervical intraepithelial lesions to invasive cancer is associated with corresponding reductions in human papillomavirus (HPV) L1 capsid antigen (L1) expression. We sought to determine whether a similar loss of L1 occurs during anal carcinogenesis using immunohistochemistry on paraffin-embedded sections as well as INNO-LiPA HPV Genotyping (Innogenetics, Gent, Belgium) technology to determine HPV infection status. We analyzed 31 squamous cell carcinomas (SCCs), 26 SCCs in situ (SCC-IS), and 11 normal anal mucosae from 36 patients. High-risk HPV subtypes were detected in all patients. L1 nuclear staining was identified in 38% of SCC-IS; however, there was no detection in normal anal mucosae, SCC, or recurrent SCC. Of those SCC-IS associated with a concomitant invasive SCC, only 15% demonstrated nuclear L1 expression as compared to 62% of isolated SCC-IS (P = .02). Nuclear expression of L1 is lost in the progression of anal SCC-IS to SCC and may serve as a possible prognostic marker of enhanced malignant potential.
authors.affiliation: Gastrointestinal Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Hernandez
authors.forename: Abul
authors.initials: A
authors.lastname: Elahi
authors.forename: Erin
authors.initials: E
authors.lastname: Siegel
authors.forename: Domenico
authors.initials: D
authors.lastname: Coppola
authors.forename: Bridgett
authors.initials: B
authors.lastname: Riggs
authors.forename: David
authors.initials: D
authors.lastname: Shibata
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001003:Anal Canal / Q000378:metabolism / Q000821:virology; D001005:Anus Neoplasms / Q000378:metabolism* / Q000821:virology; D014408:Biomarkers, Tumor / Q000378:metabolism; D036022:Capsid Proteins / Q000276:immunology / Q000378:metabolism*; D002278:Carcinoma in Situ / Q000378:metabolism* / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000821:virology; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D018450:Disease Progression; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000821:virology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D036022:Capsid Proteins; D004279:DNA, Viral; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral