title: Reduced platelet concentration does not harm PRP effectiveness for ACL repair in a porcine in vivo model.
abstract: Enhanced primary repair of the ACL using a collagen scaffold loaded with platelets has been shown to improve the functional healing of suture repair in animal models. In this study, our objectives were to determine if lowering the platelet concentration would reduce the structural properties of the repaired ACL and increase postoperative knee laxity. Eight Yucatan mini-pigs underwent bilateral suture repair. In one knee, the repair was augmented with a collagen scaffold saturated with platelet-rich plasma (PRP) containing five times the systemic baseline of platelets (5×) while the contralateral knee had a collagen scaffold saturated with PRP containing three times the systemic baseline of platelets (3×). After 13 weeks of healing, knee joint laxity and the structural properties of the ACL were measured. The 3× platelet concentration resulted in a 24.1% decrease in cellular density of the repair tissue (p < 0.05), but did not significantly decrease the structural properties [3× vs. 5×: 362 N vs. 291 N (p = 0.242) and 70 N/mm vs. 53 N/mm (p = 0.189) for the yield load and linear stiffness, respectively]. The 3× platelet concentration also did not significantly change the mean anteroposterior knee laxity at 30° and 90° of flexion [5× vs. 3×: 3.5 mm vs. 5.1 mm (p = 0.140), and 6.1 mm vs. 6.3 mm (p = 0.764)] but did result in a lower AP laxity at 60° [5× vs. 3×: 8.6 mm vs. 7.3 mm (p = 0.012)]. The decrease in platelet concentration from 5× to 3× to enhance suture repair of the ACL did not significantly harm the mechanical outcomes in this animal model.
authors.affiliation: Department of Orthopaedic Surgery, Children's Hospital of Boston, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors.forename: Ashley N
authors.initials: AN
authors.lastname: Mastrangelo
authors.forename: Patrick
authors.initials: P
authors.lastname: Vavken
authors.forename: Braden C
authors.initials: BC
authors.lastname: Fleming
authors.forename: Sophia L
authors.initials: SL
authors.lastname: Harrison
authors.forename: Martha M
authors.initials: MM
authors.lastname: Murray
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000502:physiology / Q000601:surgery; D001696:Biomechanical Phenomena / Q000502:physiology; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D005260:Female; D007719:Knee Joint / Q000502:physiology / Q000601:surgery; D008297:Male; D023421:Models, Animal; D019637:Orthopedic Procedures / Q000379:methods; D010976:Platelet Count; D053657:Platelet-Rich Plasma; D020127:Recovery of Function / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology*
chemical_list: D003094:Collagen