title: Virus trap in human serum albumin nanotube.
abstract: Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-L-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-L-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)(2)PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 × 10(7) M(-1). Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (-3.9 log order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan. komatsu@kc.chuo-u.ac.jp
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Komatsu
authors.forename: Xue
authors.initials: X
authors.lastname: Qu
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ihara
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Fujihara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Azuma
authors.forename: Hisami
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D006510:Hepatitis B Antibodies / Q000737:chemistry* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry* / Q000276:immunology
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D012709:Serum Albumin