title: Primary male infertility in Izmir/Turkey: a cytogenetic and molecular study of 187 infertile Turkish patients.
abstract: OBJECTIVE
  To detect somatic cytogenetic abnormalities and AZF microdeletions in a sample of 187 Turkish infertile men to determine the frequencies and the characteristics of our primary male infertility data in order to perform appropriate genetic counseling.
  
  
  METHODS
  This study included 187 infertile men. Chromosomal studies and screening of AZF deletions was performed by multiplex polymerase chain reaction (PCR) analysis using the Y Chromosome Deletion Detection System.
  
  
  RESULTS
  Cytogenetic study revealed chromosomal abnormality in 9 subjects (4.8%). In remaining 178 subjects, 7 subjects (3.93%) were detected to have Y chromosome microdeletions. The AZFc region was the most frequently involved region in microdeletion process in affected subjects. All subjects having microdeletion were azoospermic
  
  
  CONCLUSIONS
  Cytogenetic and molecular study should be performed to obtain reliable genetic information for the genetic counseling of primary infertile man. Y chromosome microdeletion diagnosis is useful in decision making for assisted reproductive technics.
authors.affiliation: Department of Medical Genetics School of Medicine, Ege University, Izmır, Turkey. akin63@yahoo.com
authors.forename: Haluk
authors.initials: H
authors.lastname: Akin
authors.forename: Huseyin
authors.initials: H
authors.lastname: Onay
authors.forename: Emre
authors.initials: E
authors.lastname: Turker
authors.forename: Ferda
authors.initials: F
authors.lastname: Ozkinay
mesh_terms: D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics; D003582:Cytogenetics; D005838:Genotype; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D060885:Multiplex Polymerase Chain Reaction; D010641:Phenotype; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000235:genetics*; D014421:Turkey