title: Imaging microglia in live brain slices and slice cultures.
abstract: INTRODUCTIONThis protocol describes methods for fluorescence labeling and time-lapse confocal imaging of microglia in acutely prepared tissue slices from developing and adult animals, and to slice cultures derived from early post-natal day 7 (<PND7) animals. The techniques permit real-time analyses of microglial structure and dynamic remodeling in live rodent brain tissues.
authors.forename: Dana
authors.initials: D
authors.lastname: Kurpius
authors.forename: Michael E
authors.initials: ME
authors.lastname: Dailey