title: Enhanced reactivity of Lys182 explains the limited efficacy of biogenic amines in preventing the inactivation of glucose-6-phosphate dehydrogenase by methylglyoxal.
abstract: This study examines the inactivation of the enzyme glucose 6-phosphate dehydrogenase (G6PD) by methylglyoxal (MG) and the eventual protection exerted by endogenous amines. To determine the protective effect of amines, the rate constant of the reaction of MG with the amino group of N-α-acetyl-lysine, carnosine, spermine and spermidine was measured at pH 7.4, and the behavior of endogenous amines was analyzed on the basis of quantum chemical reactivity descriptors. A 63% reduction in the enzyme activity was found upon incubation of G6PD with MG at pH 7.4. The inactivation of G6PD was even larger when the pH was increased to 9.4, revealing a weak protective effect by the amines. The results suggest that some basic residues of G6PD exhibit an anomalous reactivity, which likely reflects a shift in the standard pK(a) value due to the local environment in the enzyme. Under the experimental conditions used in the assays, this hypothesis was corroborated by mass spectrometry analysis, which points out that modification of Lys182 in the binding site is responsible for the inactivation of G6PD by MG. These results emphasize the need to search for more effective antiglycating agents, which can compete with basic amino acid residues possessing enhanced reactivity in proteins.
authors.affiliation: Departament de Fisicoquímica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Patricio
authors.initials: P
authors.lastname: Flores-Morales
authors.forename: Claudio
authors.initials: C
authors.lastname: Diema
authors.forename: Marta
authors.initials: M
authors.lastname: Vilaseca
authors.forename: Joan
authors.initials: J
authors.lastname: Estelrich
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Luque
authors.forename: Soledad
authors.initials: S
authors.lastname: Gutiérrez-Oliva
authors.forename: Alejandro
authors.initials: A
authors.lastname: Toro-Labbé
authors.forename: Eduardo
authors.initials: E
authors.lastname: Silva
mesh_terms: D001679:Biogenic Amines / Q000737:chemistry / Q000502:physiology*; D004789:Enzyme Activation / Q000187:drug effects*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008239:Lysine / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D011765:Pyruvaldehyde / Q000494:pharmacology*
chemical_list: D001679:Biogenic Amines; D011765:Pyruvaldehyde; D005954:Glucosephosphate Dehydrogenase; D008239:Lysine