title: Effect of growth media and washing on the spectral signatures of aerosolized biological simulants.
abstract: We have evaluated the influence of growth media and washing on the laser-induced fluorescence spectra of bacteria. Three different bacterial simulants were cultured in three types of growth media. Three kinds of samples were generated from each culture: the culture itself, the growth medium alone, and a triple-washed sample. The materials were injected as aerosols in a lab-sized lidar aerosol chamber to obtain their spectra. Using two different analysis approaches, signature variations were observed between the three kinds of samples for most combinations of growth media/bacteria. This study concludes that the culture media used influences the spectral signatures.
authors.affiliation: Tactical Surveillance and Reconnaissance Section, Defence Research & Development Canada Valcartier, Quebec City, Quebec, Canada. christian.laflamme@drdc‐rddc.gc.ca
authors.forename: Christian
authors.initials: C
authors.lastname: Laflamme
authors.forename: Jean-Robert
authors.initials: JR
authors.lastname: Simard
authors.forename: Sylvie
authors.initials: S
authors.lastname: Buteau
authors.forename: Pierre
authors.initials: P
authors.lastname: Lahaie
authors.forename: Denis
authors.initials: D
authors.lastname: Nadeau
authors.forename: Bernard
authors.initials: B
authors.lastname: Déry
authors.forename: Olivier
authors.initials: O
authors.lastname: Houle
authors.forename: Pierre
authors.initials: P
authors.lastname: Mathieu
authors.forename: Gilles
authors.initials: G
authors.lastname: Roy
authors.forename: Jim
authors.initials: J
authors.lastname: Ho
authors.forename: Caroline
authors.initials: C
authors.lastname: Duchaine
mesh_terms: D000336:Aerosols / Q000737:chemistry*; D001407:Bacillus / Q000737:chemistry / Q000378:metabolism; D054045:Biological Warfare Agents; D017077:Culture Media, Conditioned / Q000737:chemistry*; D004885:Erwinia / Q000737:chemistry / Q000378:metabolism; D010363:Pattern Recognition, Automated / Q000379:methods; D025341:Principal Component Analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000336:Aerosols; D054045:Biological Warfare Agents; D017077:Culture Media, Conditioned