title: [Observation on osteoblasts responded to fluid induced shear cultured on different surfaces with scanning electron microscope].
abstract: OBJECTIVE
  To investigate morphological change of osteoblasts cultured on titanium plates with different microarchitecture structure when exposured to fluid shear stress.
  
  
  METHODS
  14 dynes x cm(-2) fluid shear stress was applied on osteoblasts cultured on 3 different commercially pure titanium plates: Polished treatment (PT), sandblast (SB), sandblasting and acid-base (SB-AB) surfaces. Scanning electron microscope (SEM) was adopted to observe the morphological changes after 0.5, 4, 7.5 h time point respectively.
  
  
  RESULTS
  Morphologically, no significant changes were observed after 0.5 h and few osteoblasts were seen after 7.5 h on all 3 type of different surfaces, and significant changes could only be observed after 4 h. Osteoblasts were elongated and rearranged along the flow way on different levels on PT surface. Shape of cells was altered, from long fusiform suspending over depressed areas into polygon stretching out many synapsises tightly attached to pits on SB-AB surface. Osteoblasts on SB surface displayed similar change as SB-AB surface, besides, some cells were elongated along the way of flow, stretching out threadlike synapsises attached to edges of pits.
  
  
  CONCLUSIONS
  Morphological change of osteoblast responding to fluid shear stress in physiological range depends on substrate microarchitecture and varies with the time of fluid shear stress application.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Li
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yang
authors.forename: Su-Qin
authors.initials: SQ
authors.lastname: Xian
mesh_terms: D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts; D013314:Stress, Mechanical; D014025:Titanium
chemical_list: D014025:Titanium