title: Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe).
abstract: A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62(T), was isolated during a study of culturable bacteria from the gut of Moechotypa diphysis (Pascoe) and its taxonomic position was investigated. Strain RB-62(T) grew at 15-30 °C and pH 5.0-8.5. The isoprenoid quinones were menaquinones MK-11 (77.1%), MK-10 (11.7%) and MK-12 (11.2%). The major cellular fatty acids were anteiso-C(15:0) (34.6%), anteiso-C(17:0) (29.8%), iso-C(16:0) (17.0%) and cyclohexyl-C(17:0) (11.4%). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62(T) was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62(T) was affiliated with the genus Herbiconiux cluster within the family Microbacteriaceae, and was related most closely to Herbiconiux ginsengi wged11(T) (98.08% similarity). The level of DNA-DNA relatedness between strain RB-62(T) and H. ginsengi wged11(T) was 43.2% (reciprocal 66.7%). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62(T) from recognized species of the genus Herbiconiux. Based on data from the present polyphasic study, strain RB-62(T) is considered to represent a novel species of the genus Herbiconiux, for which the name Herbiconiux moechotypicola sp. nov. is proposed. The type strain is RB-62(T) (=KCTC 19653(T)=JCM 16117(T)).
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Byung-Chun
authors.initials: BC
authors.lastname: Kim
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Hyangmi
authors.initials: H
authors.lastname: Kim
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Oh
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
authors.affiliation: Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D001517:Coleoptera / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041981:Gastrointestinal Tract / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature; D014990:Xylans / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D014990:Xylans