title: Protein expression profiles of C3H 10T1/2 murine fibroblasts and of isogenic cells transformed by the H1047R mutant of phosphoinositide 3-kinase (PI3K).
abstract: We have used stable isotope labeling with amino acids in cell culture (SILAC) in conjunction with tandem mass spectrometry to characterize the proteomes of two isogenic cell lines that differ in the expression of a single oncoprotein,p110α of PI3K, carrying the H1047R mutation. 51,510 peptides were identified and assigned to 4,201 proteins. Most notable among the proteins that show increased expression in the oncogenically transformed cells are several involved in the interferon response including Isg15, Ifit1, Igtp and Oas2 (interferon stimulated gene 15, interferon-induced protein with tetratricopeptide repeats 1, interferon gamma-inducible GTP-binding protein, 2'-5'-oligoadenylate synthetase 2). Prominent among the downregulated proteins are several involved in cell adhesion as well as proteins that are affected by the negative feedback from PI3K signaling. The differential expressions documented in this analysis suggest novel links between oncogenic PI3K and several signaling pathways. These links will be explored in future studies.
authors.affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA. jhart@scripps.edu
authors.forename: Jonathan Ross
authors.initials: JR
authors.lastname: Hart
authors.forename: Lujian
authors.initials: L
authors.lastname: Liao
authors.forename: Lynn
authors.initials: L
authors.lastname: Ueno
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Peter K
authors.initials: PK
authors.lastname: Vogt
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D020134:Catalytic Domain; D002461:Cell Line, Transformed; D002851:Chromatography, High Pressure Liquid; D015536:Down-Regulation; D005347:Fibroblasts / Q000378:metabolism*; D007553:Isotope Labeling; D051379:Mice; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry; D014162:Transfection; D015854:Up-Regulation
chemical_list: D020543:Proteome; D019869:Phosphatidylinositol 3-Kinases