title: Carbohydrate nanoparticle-mediated colloidal assembly for prolonged efficacy of bacteriocin against food pathogen.
abstract: The goal of this study was to demonstrate the ability of carbohydrate nanoparticle-stabilized emulsion to prolong the efficacy of bacteriocin against food pathogens. An amphiphilic, negatively charged carbohydrate nanoparticle, phytoglycogen octenyl succinate (PG-OS), was used to form oil-in-water emulsion for delivering bacteriocin nisin against the food pathogen Listeria monocytogenes. Dynamic light scattering test showed that in emulsion all PG-OS nanoparticles were adsorbed at the surface of oil droplets. Zeta-potential analysis indicated an effective adsorption of positively charged nisin molecules at the surface of PG-OS interfacial layer. Nisin depletion model showed that, during 50 days of storage, the anti-listerial activity of nisin-containing PG-OS-stabilized emulsion was substantially greater than that of nisin solution. In contrast, the emulsion stabilized with a neutral, small-molecule surfactant (Tween 20) or negatively charged, hyperbranched carbohydrate polymer (modified starch) was either ineffective or less effective than the nanoparticle-stabilized emulsion to retain nisin activity during storage.
authors.affiliation: Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Bi
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Arun K
authors.initials: AK
authors.lastname: Bhunia
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D003102:Colloids / Q000378:metabolism*; D015169:Colony Count, Microbial; D004337:Drug Carriers / Q000378:metabolism*; D004655:Emulsions / Q000378:metabolism / Q000494:pharmacology; D005516:Food Microbiology; D006003:Glycogen / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D053758:Nanoparticles; D009561:Nisin / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003102:Colloids; D004337:Drug Carriers; D004655:Emulsions; D009561:Nisin; D006003:Glycogen