title: Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing.
abstract: Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major parameter controlling this sensitivity. By careful optimizing the laser system, the high-quality vibrational spectra acquisition at the multi-kHz rate becomes feasible.
authors.affiliation: University of Wisconsin - Milwaukee, Department of Physics, 1900 E. Kenwood Boulevard, Milwaukee, Wisconsin 53211, USA.
authors.forename: Rajan
authors.initials: R
authors.lastname: Arora
authors.forename: Georgi I
authors.initials: GI
authors.lastname: Petrov
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Yakovlev
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007834:Lasers; D008853:Microscopy / Q000295:instrumentation*; D017711:Nonlinear Dynamics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*