title: A multisite performance study comparing the reading of immunohistochemical slides on a computer monitor with conventional manual microscopy for estrogen and progesterone receptor analysis.
abstract: A multisite study was conducted to assess the performance of the Aperio digital pathology system (Aperio Technologies, Vista, CA) for reading estrogen receptor (ER) and progesterone receptor (PR) slides on a computer monitor. A total of 520 formalin-fixed breast tissue specimens were assayed at 3 clinical sites for ER and PR (260 each). Percentage and average staining intensity of positive nuclei were assessed. At each site, 3 pathologists performed a blinded reading of the glass slides using their microscopes initially and later using digital images on a computer monitor. Comparable percentages of agreements were obtained for manual microscopy (MM) and manual digital slide reading (MDR) (ER, percentage of positive nuclei with cutoffs: MM, 91.3%-99.0%/MDR, 91.3%-100.0%; PR, percentage of positive nuclei with cutoffs: MM, 83.8%-99.0%/MDR, 76.3%-100.0%). Reading ER and PR slides on a computer monitor using the Aperio digital pathology system is equivalent to reading the slides with a conventional light microscope.
authors.affiliation: Pathology and Laboratory Medicine, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Aziza
authors.initials: A
authors.lastname: Nassar
authors.forename: Cynthia
authors.initials: C
authors.lastname: Cohen
authors.forename: Sally S
authors.initials: SS
authors.lastname: Agersborg
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Lynch
authors.forename: Edward A
authors.initials: EA
authors.lastname: Barker
authors.forename: Burton L
authors.initials: BL
authors.lastname: Vanderbilt
authors.forename: Jim
authors.initials: J
authors.lastname: Thompson
authors.forename: Eugene R
authors.initials: ER
authors.lastname: Heyman
authors.forename: Allen
authors.initials: A
authors.lastname: Olson
authors.forename: Holger
authors.initials: H
authors.lastname: Lange
authors.forename: Momin T
authors.initials: MT
authors.lastname: Siddiqui
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007150:Immunohistochemistry; D008853:Microscopy / Q000379:methods*; D015588:Observer Variation; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D015203:Reproducibility of Results; D019113:Telepathology
chemical_list: D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone