title: DNA-based optomechanical molecular motor.
abstract: An azobenzene-capped DNA hairpin coupled to an AFM is presented as an optically triggered single-molecule motor. The photoinduced trans to cis isomerization of azobenzene affects both the overall length of the molecule and the ability of the DNA bases to hybridize. Using a combination of molecular dynamics simulations and free energy calculations the unfolding of both isomers along the O5'-O3' extension coordinate is monitored. The potentials of mean force (PMFs) along this coordinate indicate that there are two major differences induced by photoisomerization. The first is that the interbase hydrogen bond and stacking interactions are stable for a greater range of extensions in the trans system than in the cis system. The second difference is due to a decreased chain length of the cis isomer with respect to the trans isomer. These differences are exploited to extract work in optomechanical cycles. The disruption of the hairpin structure gives a maximum of 3.4 kcal mol(-1) of extractable work per cycle with an estimated maximum efficiency of 2.4%. Structure-function insights into the operation of this motor are provided, and the effect of the cantilever stiffness on the extractable work is characterized.
authors.affiliation: Department of Chemistry, Northwestern University, Evanston, Illinois 60208-3113, United States.
authors.forename: Martin
authors.initials: M
authors.lastname: McCullagh
authors.forename: Ignacio
authors.initials: I
authors.lastname: Franco
authors.forename: Mark A
authors.initials: MA
authors.lastname: Ratner
authors.forename: George C
authors.initials: GC
authors.lastname: Schatz
mesh_terms: D004247:DNA / Q000737:chemistry*; D004548:Elasticity; D019277:Entropy; D055595:Mechanical Phenomena; D018625:Microscopy, Atomic Force; D056004:Molecular Dynamics Simulation; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA