title: Mometasone and desloratadine additive effect on eosinophil survival and cytokine secretion from epithelial cells.
abstract: BACKGROUND
  Although antihistamines and topical corticosteroids are used in combination to treat allergic rhinitis, their additive effect has not been yet demonstrated. The aim was investigate the antiinflammatory additive effect of mometasone and desloratadine on cytokine and sICAM-1 secretion by epithelial cells, and on eosinophil survival stimulated by human epithelial cells secretions from nasal mucosa and polyps.
  
  
  METHODS
  Epithelial cells obtained from nasal mucosa or polyps were stimulated with 10% fetal bovine serum in presence of mometasone (10(-11) M-10(-5) M) with/without desloratadine (10(-5) M). Cytokine and sICAM-1 concentrations in supernatants were measured by ELISA. Peripheral blood eosinophils were incubated during 4 days with epithelial cell secretions with (10(-11) M-10(-5) M) and/or desloratadine (10(-5) M) and survival assessed by Trypan blue. Results are expressed as percentage (mean ± SEM) compared to control.
  
  
  RESULTS
  Fetal bovine serum stimulated IL-6, IL-8, GM-CSF and sICAM-1 secretion. In mucosa and polyp epithelial cells, mometasone inhibited this induced secretion while desloratadine inhibited IL-6 and IL-8. The combination of 10(-5) M desloratadine and 10(-9) M mometasone reduced IL-6 secretion (48 ± 11%, p < 0.05) greater extent than mometasone alone (68 ± 10%) compared to control (100%). Epithelial cell secretions induced eosinophil survival from day 1 to 4, this effect being inhibited by mometasone. At day 4, the combination of mometasone (10(-11) M) and desloratadine (10(-5) M) provoked an increased inhibition of eosinophil survival induced by cell secretions (27 ± 5%, p < 0.01) than mometasone (44 ± 7%) or desloratadine (46 ± 7%) alone.
  
  
  CONCLUSIONS
  These results suggest that the combination of desloratadine and mometasone furoate have a greater antinflammatory effect in an in vitro model of eosinophil inflammation than those drugs administered alone.
authors.affiliation: IDIBAPS, Hospital Clínic, CIBER de Enfermedades Respiratorias (CIBERES), Villarroel 170, Barcelona, 08036, Catalonia, Spain.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Mullol
authors.forename: Francisco
authors.initials: F
authors.lastname: de Borja Callejas
authors.forename: Maria Asunción
authors.initials: MA
authors.lastname: Martínez-Antón
authors.forename: Eva
authors.initials: E
authors.lastname: Méndez-Arancibia
authors.forename: Isam
authors.initials: I
authors.lastname: Alobid
authors.forename: Laura
authors.initials: L
authors.lastname: Pujols
authors.forename: Antonio
authors.initials: A
authors.lastname: Valero
authors.forename: Cesar
authors.initials: C
authors.lastname: Picado
authors.forename: Jordi
authors.initials: J
authors.lastname: Roca-Ferrer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D017336:Loratadine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D000068656:Mometasone Furoate; D009297:Nasal Mucosa / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D009298:Nasal Polyps / Q000276:immunology* / Q000473:pathology; D019899:Paracrine Communication / Q000187:drug effects; D011244:Pregnadienediols / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; C508606:CXCL8 protein, human; D016207:Cytokines; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D011244:Pregnadienediols; D000068656:Mometasone Furoate; D018799:Intercellular Adhesion Molecule-1; D017336:Loratadine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C121345:desloratadine