title: Optical sectioning with a low-coherence phase-shifting digital holographic microscope.
abstract: The properties of a low-coherence phase-shifting digital holographic microscope are first studied and analyzed. We then demonstrate en face imaging with transverse resolution of 3 μm and axial resolution of 10 μm through a thickness of 300 μm onion membrane. In addition, with the above said resolutions, optical sectioning of the eye and spine of a live zebra fish has been demonstrated. To the best of our knowledge, this is the first time that a short coherence phase-shifting holographic microscope has been applied to the internal structure visualization of a biological specimen under an in vivo environment.
authors.affiliation: Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 11677, Taiwan.
authors.forename: Yu-Chih
authors.initials: YC
authors.lastname: Lin
authors.forename: Chau-Jern
authors.initials: CJ
authors.lastname: Cheng
authors.forename: Ting-Chung
authors.initials: TC
authors.lastname: Poon