title: Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae.
abstract: BACKGROUND
  To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007.
  
  
  METHODS
  Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR.
  
  
  RESULTS
  Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes.
  
  
  CONCLUSIONS
  Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.
authors.affiliation: Municipal Health Secretariat of Belo Horizonte, Belo Horizonte, MG, Brazil. edumpessanha@hotmail.com
authors.forename: José Eduardo Marques
authors.initials: JE
authors.lastname: Pessanha
authors.forename: Waleska Teixeira
authors.initials: WT
authors.lastname: Caiaffa
authors.forename: Alzira Batista
authors.initials: AB
authors.lastname: Cecilio
authors.forename: Felipe Campos de Melo
authors.initials: FC
authors.lastname: Iani
authors.forename: Simone Costa
authors.initials: SC
authors.lastname: Araujo
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Nascimento
authors.forename: Erna Geessien
authors.initials: EG
authors.lastname: Kroon
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Proietti
authors.forename: Jorge Rodriguez
authors.initials: JR
authors.lastname: Arias
mesh_terms: D000330:Aedes / Q000145:classification / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000635:transmission / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007303:Insect Vectors / Q000145:classification / Q000821:virology*; D007814:Larva / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral