title: Palladium-mediated intracellular chemistry.
abstract: Many important intracellular biochemical reactions are modulated by transition metals, typically in the form of metalloproteins. The ability to carry out selective transformations inside a cell would allow researchers to manipulate or interrogate innumerable biological processes. Here, we show that palladium nanoparticles trapped within polystyrene microspheres can enter cells and mediate a variety of Pd(0)-catalysed reactions, such as allylcarbamate cleavage and Suzuki-Miyaura cross-coupling. The work provides the basis for the customization of heterogeneous unnatural catalysts as tools to carry out artificial chemistries within cells. Such in cellulo synthesis has potential for a plethora of applications ranging from cellular labelling to synthesis of modulators or inhibitors of cell function.
authors.affiliation: School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK.
authors.forename: Rahimi M
authors.initials: RM
authors.lastname: Yusop
authors.forename: Asier
authors.initials: A
authors.lastname: Unciti-Broceta
authors.forename: Emma M V
authors.initials: EM
authors.lastname: Johansson
authors.forename: Rosario M
authors.initials: RM
authors.lastname: Sánchez-Martín
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D002219:Carbamates / Q000737:chemistry; D002384:Catalysis; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D002219:Carbamates; D011137:Polystyrenes; D010165:Palladium