title: Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics.
abstract: ROS, including hydrogen peroxide (H(2)O(2)), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H(2)O(2) or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H(2)O(2), led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
authors.forename: Sophie
authors.initials: S
authors.lastname: Alvarez
authors.forename: Ashley
authors.initials: A
authors.lastname: Galant
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Jez
authors.forename: Leslie M
authors.initials: LM
authors.lastname: Hicks
mesh_terms: D019328:Buthionine Sulfoximine / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009149:Mustard Plant / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D019328:Buthionine Sulfoximine; D006861:Hydrogen Peroxide; D005978:Glutathione