title: Association of SRC-related kinase Lyn with the interleukin-2 receptor and its role in maintaining constitutive phosphorylation of JAK/STAT in human T-cell leukemia virus type 1-transformed T cells.
abstract: Human T-cell leukemia virus type 1 (HTLV-1) infection and transformation are associated with an incremental switch in the expression of the Src-related protein tyrosine kinases Lck and Lyn. We examined the physical and functional interactions of Lyn with receptors and signal transduction proteins in HTLV-1-infected T cells. Lyn coimmunoprecipitates with the interleukin-2 beta receptor (IL-2Rβ) and JAK3 proteins; however, the association of Lyn with the IL-2Rβ and Lyn kinase activity was independent of IL-2 stimulation. Phosphorylation of Janus kinase 3 (JAK3) and signal transducers and activator of transcription 5 (STAT5) proteins was reduced by treatment of cells with the Src kinase inhibitor PP2 or by ectopic expression of a dominant negative Lyn kinase protein.
authors.affiliation: Division of Basic Pharmaceutical Sciences, College of Pharmacy, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA. mshuh@xula.edu
authors.forename: Maureen
authors.initials: M
authors.lastname: Shuh
authors.forename: Barry A
authors.initials: BA
authors.lastname: Morse
authors.forename: Gisela
authors.initials: G
authors.lastname: Heidecker
authors.forename: David
authors.initials: D
authors.lastname: Derse
mesh_terms: D002472:Cell Transformation, Viral; D054884:Host-Pathogen Interactions; D015368:Human T-lymphotropic virus 1 / Q000472:pathogenicity*; D006801:Humans; D047468:Immunoprecipitation; D053646:Interleukin-2 Receptor beta Subunit / Q000378:metabolism*; D053616:Janus Kinase 3 / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D050791:STAT Transcription Factors / Q000378:metabolism*; D013601:T-Lymphocytes / Q000821:virology*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D053646:Interleukin-2 Receptor beta Subunit; D050791:STAT Transcription Factors; D053616:Janus Kinase 3; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases