title: Adrenoceptor stimulation does not affect ICAM-1 and VCAM-1 expression in vitro.
abstract: BACKGROUND
  Endothelial adhesion molecules ICAM-1 (CD54) and VCAM-1 (CD106) mediate cellular adhesion and transcellular migration. Cell adhesion and diapedesis have a key role in the course of shock and sepsis. During severe sepsis, adrenoceptor agonist levels may be increased due to endogenous production or due to intensive care treatment. As yet, the influence of β1 or β2 agonists on adhesion molecule formation on endothelial cells has remained unclear.
  
  
  METHODS
  Cultured human umbilical vein endothelial cells were stimulated with E. coli. Following bacterial stimulation the cells were incubated with either β2 receptor agonist terbutaline or β1 agonist norepinephrine. ICAM-1 and VCAM-1 expression were examined using flow cytometry.
  
  
  RESULTS
  Administration of norepinephrine did not cause increases of both CD54 and CD106 in stimulated HUVEC. Compared to negative controls the bacterial stimulation itself led to an increase of adhesion molecules. Following administration of terbutaline no significant increase in CD54 expression was found.
  
  
  CONCLUSIONS
  Bacterial stimulation led to an increase of adhesion molecule expression. Adrenoceptor stimulation of activated endothelial cells did not cause significant increases of cellular adhesion molecules.
authors.affiliation: Dept, of Anaesthesiology & Intensive Care Medicine, Hannover Medical School Hannover, Germany. ruschulte.heiner@mh-hannover.de.
authors.forename: Heiner
authors.initials: H
authors.lastname: Ruschulte
authors.forename: Dirk
authors.initials: D
authors.lastname: Scheinichen
authors.forename: Martijn
authors.initials: M
authors.lastname: van Griensven
authors.forename: Susanne
authors.initials: S
authors.lastname: Weyrauch
authors.forename: Wibke
authors.initials: W
authors.lastname: Liefing
authors.forename: Birgitt
authors.initials: B
authors.lastname: Harrmeijer
authors.forename: Michael
authors.initials: M
authors.lastname: Przemeck
authors.forename: Björn
authors.initials: B
authors.lastname: Jüttner