title: [Genetic polymorphism of XRCC1 associated with susceptibility of chromosomal damage in workers exposed by 1,3-butadiene].
abstract: OBJECTIVE
  To explore the relationship between genetic polymorphism of XRCC1 194, 280, 399 and susceptibility of chromosomal damage induced by 1,3-butadiene (BD).
  
  
  METHODS
  138 workers occupationally exposed to BD and 41 normal individuals were involved. Cytokinesis-block micronucleus (CB-MN) assay was used to detect chromosome damage in peripheral lymphocyte. PCR-RFLP technique was applied to detect polymorphisms in XRCC1 194, 280 and 399.
  
  
  RESULTS
  The MN frequencies (3.39 +/- 2.42) per thousand of workers exposed to BD were more higher than those of the non-exposed groups (1.48 +/- 1.26) per thousand (P < 0.01). Workers receiving mere exposures had higher MN values than those of lower-exposed workers: frequency ratios (FR) = 1.30 (95% CI 1.14 - 1.53, P < 0.05). Workers with XRCC1 194 Arg/Trp genotype had more susceptibility for chromosome damage, FR = 1.13 (95% CI 1.07 - 1.27). Workers with XRCC1 280 Arg/His genotype had more susceptibility for chromosome damage, FR = 1.67 (95% CI 1.10 - 2.42). XRCC1 399 Arg/Gln and combined group of Arg/Gln and Gln/Gln genotype carrier had higher MN frequency, FR = 1.26 (95% CI 1.03 - 1.53) and 1.24 (95% CI 1.03 - 1.49) respectively. The haplotypes CAG/TGG could associate with the decreased frequencies of total micronucleus (P < 0.05).
  
  
  CONCLUSIONS
  Genotype of XRCC1 could associated with the chromosome damage induced by BD.
authors.affiliation: School of Public Health, Fudan University, MO Key Lab for Public Health Safety, Shanghai 200032, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Hongshan
authors.initials: H
authors.lastname: Tan
authors.forename: Aihong
authors.initials: A
authors.lastname: Wang
authors.forename: Nannan
authors.initials: N
authors.lastname: Feng
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Ye
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Feng
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000328:Adult; D002070:Butadienes / Q000633:toxicity*; D002617:Chemical Industry; D002681:China; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D059647:Gene-Environment Interaction; D005838:Genotype; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D016273:Occupational Exposure / Q000009:adverse effects; D011110:Polymorphism, Genetic; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D002070:Butadienes; D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; C031763:1,3-butadiene