title: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress.
abstract: Mitogen-activated protein kinase (MAPK) cascades play important roles in mediating pathogen responses and reactive oxygen species signaling. In plants, MAPKs are classified into four major groups (A-D). Previous studies have mainly focused on groups A and B, but little is known about group C. In this study, we functionally characterized a stress-responsive group C MAPK gene (GhMPK2) from cotton. Northern blot analysis indicated that GhMPK2 was induced not only by signaling molecules, such as ethylene and methyl jasmonate, but also by methyl viologen-mediated oxidative stress. Transgenic tobacco (Nicotiana tabacum) plants that overexpress GhMPK2 displayed enhanced resistance to fungal and viral pathogens, and the expression of the pathogenesis-related (PR) genes, including PR1, PR2, PR4, and PR5, was significantly increased. Interestingly, the transcription of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) was significantly upregulated in transgenic plants, suggesting that GhMPK2 positively regulates ethylene synthesis. Moreover, overexpression of GhMPK2 elevated the expression of several antioxidant enzymes, conferring on transgenic plants enhanced reactive oxygen species scavenging capability and oxidative stress tolerance. These results increased our understanding of the role of the group C GhMPK2 gene in multiple defense-signaling pathways, including those that are involved in responses to pathogen infection and oxidative stress.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xi
authors.forename: Lu
authors.initials: L
authors.lastname: Luo
authors.forename: Fei
authors.initials: F
authors.lastname: Meng
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Li
authors.forename: Chang-ai
authors.initials: CA
authors.lastname: Wu
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis; D003517:Cyclopentanes / Q000494:pharmacology; D004790:Enzyme Induction; D005030:Ethylenes / Q000494:pharmacology; D005670:Fusarium / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000201:enzymology*; D008190:Lyases / Q000096:biosynthesis; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D018384:Oxidative Stress; D054883:Oxylipins / Q000494:pharmacology; D010838:Phytophthora / Q000472:pathogenicity; D010935:Plant Diseases / Q000517:prevention & control* / Q000821:virology; D030821:Plants, Genetically Modified / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D017382:Reactive Oxygen Species; C072239:methyl jasmonate; C036216:ethylene; D000594:Amino Acid Oxidoreductases; C059460:1-aminocyclopropane-1-carboxylic acid oxidase; D020928:Mitogen-Activated Protein Kinases; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase