title: Microscale plasma-initiated patterning of electrospun polymer scaffolds.
abstract: Microscale plasma-initiated patterning (μPIP) is a novel micropatterning technique used to create biomolecular micropatterns on polymer surfaces. The patterning method uses a polydimethylsiloxane (PDMS) stamp to selectively protect regions of an underlying substrate from oxygen plasma treatment resulting in hydrophobic and hydrophilic regions. Preferential adsorption of the biomolecules onto either the plasma-exposed (hydrophilic) or plasma-protected (hydrophobic) regions leads to the biomolecular micropatterns. In the current work, laminin-1 was applied to an electrospun polyamide nanofibrillar matrix following plasma treatment. Radial glial clones (neural precursors) selectively adhered to these patterned matrices following the contours of proteins on the surface. This work demonstrates that textured surfaces, such as nanofibrillar scaffolds, can be micropatterned to provide external chemical cues for cellular organization.
authors.affiliation: Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, United States.
authors.forename: Roberto
authors.initials: R
authors.lastname: Delgado-Rivera
authors.forename: Jeremy
authors.initials: J
authors.lastname: Griffin
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Ricupero
authors.forename: Martin
authors.initials: M
authors.lastname: Grumet
authors.forename: Sally
authors.initials: S
authors.lastname: Meiners
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Uhrich
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D007797:Laminin / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010949:Plasma / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D007797:Laminin; D011108:Polymers; C121346:laminin 1; C013830:baysilon