title: Vaccine farming in Cape Town.
abstract: The review details the development of the Subunit Vaccine Group at the University of Cape Town, from its beginnings as a plant virology laboratory in the 1980s. The investigation and development of Human papillomavirus (HPV) and Human immunodeficiency vaccine candidates are covered in detail, with an emphasis on how this work allowed the evolution of a systematic approach to the optimisation of expression of these and other proteins especially in plants, but also in insect cell culture. We discuss various insights gained during our work, such as approaches to codon optimisation, use of different vector systems and plant hosts, intracellular targetting and gene modification. The future prospects for both our work and for the field of plant-made vaccines in general, are discussed.
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa. ed.rybicki@uct.ac.za
authors.forename: Edward P
authors.initials: EP
authors.lastname: Rybicki
authors.forename: Anna-Lise
authors.initials: AL
authors.lastname: Williamson
authors.forename: Ann
authors.initials: A
authors.lastname: Meyers
authors.forename: Inga I
authors.initials: II
authors.lastname: Hitzeroth
mesh_terms: D016915:AIDS Vaccines / Q000096:biosynthesis; D000818:Animals; D017791:Geminiviridae / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D027383:Papillomaviridae / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000096:biosynthesis; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011817:Rabbits; D013019:South Africa; D022223:Vaccines, Subunit; D014765:Viral Vaccines / Q000096:biosynthesis*
chemical_list: D016915:AIDS Vaccines; D053918:Papillomavirus Vaccines; D022223:Vaccines, Subunit; D014765:Viral Vaccines