title: [Effect of transglutaminase-gene disruption in Streptomyces hygroscopicus on cell differentiation].
abstract: OBJECTIVE
  To provide theoretical basis for understanding the physiological function of transglutaminase in Streptomyces, which would benefit for improving the production of this enzyme, transglutaminase gene was disrupted in Streptomyces hygroscopicus.
  
  
  METHODS
  First, the interruption plasmid pKC1139-TG1 was constructed by inserting a segment of transglutaminase gene in the temperature-sensitive vector pKC1139. Second, pKC1139-TG1 was transformed into S. hygroscopicus protoplast and the interruption plasmid was inserted into the chromosome by homogenous recombination. Third, the strain with disrupted transglutaminase gene was screened by apramycin resistance and named as S. h-delta TG.
  
  
  RESULTS
  Compared with the parent strain, S. h-delta TG was able to grow as substrate mycelium, but not able to form aerial hyphae.
  
  
  CONCLUSIONS
  Transglutaminase may be involved in the aerial hyphae formation of S. hygroscopicus.
authors.affiliation: State Key Laboratory of Food Science and Technology, Ministry of Education, Jiangnan University, Wuxi 214122, China. jnckkang@126.com
authors.forename: Kangkang
authors.initials: K
authors.lastname: Chen
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ju
authors.forename: Tengbo
authors.initials: T
authors.lastname: Ma
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhang
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D025301:Hyphae / Q000502:physiology; D010957:Plasmids; D013302:Streptomyces / Q000166:cytology / Q000201:enzymology*; D011503:Transglutaminases / Q000235:genetics / Q000502:physiology*
chemical_list: D011503:Transglutaminases