title: Synthesis and in vitro anti-HSV-1 activity of a novel Hsp90 inhibitor BJ-B11.
abstract: In this study, a novel Hsp90 inhibitor BJ-B11, was synthesized and evaluated for in vitro antiviral activity against several viruses. Possible anti-HSV-1 mechanisms were also investigated. BJ-B11 displayed no antiviral activity against coxsackievirus B(3) (CVB(3)), human respiratory syncytial virus (RSV) and influenza virus (H1N1), but exhibited potent anti-HSV-1 and HSV-2 activity with EC(50) values of 0.42±0.18 μM and 0.60±0.21 μM, respectively. Additionally, the inhibitory effects of BJ-B11 against HSV-1 were likely to be introduced at early stage of infection. Our results indicate that BJ-B11 with alternative mechanisms of action is potent as an anti-HSV clinical trial candidate.
authors.affiliation: Guangzhou Jinan Biomedicine Research and Development Center, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China.
authors.forename: Huai-Qiang
authors.initials: HQ
authors.lastname: Ju
authors.forename: Yang-Fei
authors.initials: YF
authors.lastname: Xiang
authors.forename: Bao-Juan
authors.initials: BJ
authors.lastname: Xin
authors.forename: Ying
authors.initials: Y
authors.lastname: Pei
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Lu
authors.forename: Qiao-Li
authors.initials: QL
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Xia
authors.forename: Chui-Wen
authors.initials: CW
authors.lastname: Qian
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ren
authors.forename: Sha-Yan
authors.initials: SY
authors.lastname: Wang
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Guo-Wen
authors.initials: GW
authors.lastname: Xing
mesh_terms: D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D001549:Benzamides / Q000138:chemical synthesis* / Q000494:pharmacology*; D018841:HSP90 Heat-Shock Proteins / Q000138:chemical synthesis* / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics; D007191:Indazoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction
chemical_list: C559135:2-(4-acetyloxycyclohexylamino)-4-(3,6,6-trimethyl-4-oxo-4,5,6,7-tetrahydro-1H-indazol-1-yl)-benzamide; D000998:Antiviral Agents; D001549:Benzamides; D018841:HSP90 Heat-Shock Proteins; D007191:Indazoles