title: [Effects of verapamil on the proliferation and migration of cervical cancer cells].
abstract: OBJECTIVE
  To investigate the effects of Verapamil, a L-type calcium channel blocker, on the proliferation and migration of human cervical cancer cells.
  
  
  METHODS
  Human cervical cancer cell lines (HeLa cells) were incubated with serum-free media for 24 hours. These cells were then treated with Verapamil for different time points. Cell proliferation was evaluated by MTT assay and EdU fluorescent assay. The migration of cells was measured by the wound healing assay. Results MTT assay demonstrated that, Verapamil (1, 5, 10 and 20 microg/mL concentrations) could inhibit the proliferation of HeLa cells after 48 or 72 hours treatment. The inhibition rate for 48 h-treatment was 20.87% +/- 1.71%, 23.55% +/- 4.46%, 28.65% +/- 1.32%, 27.37% +/- 3.05% respectively (P < 0.05). After 5, 10 and 20 microg/mL concentrations of verapamil treatment for 24h, the proportion of HeLa cells in the stage of DNA synthesis was 15.7% +/- 1.2%, 11.7% +/- 0.3%, 2.8% +/- 0.5% as evaluated with EdU fluorescent assay, which was significantly lower than that of control (27.34% +/- 2.1%, P < 0.05). With the increase of drug concentrations, Verapamil could significantly suppressed the DNA synthesis in HeLa cell. Cell migration assay revealed that, treatment with Verapamil (5, 10 and 20 microg/mL) for 24 hours significantly inhibited cells migration distance (P < 0.05).
  
  
  CONCLUSIONS
  Calcium channel blocker Verapamil could inhibit proliferation and migration of the cervical cancer HeLa cells.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wan
authors.forename: Yan-xia
authors.initials: YX
authors.lastname: Chu
authors.forename: Ai
authors.initials: A
authors.lastname: Zheng
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Liu
authors.forename: Wen-chao
authors.initials: WC
authors.lastname: Wu
mesh_terms: D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D014700:Verapamil