title: The SUUR protein is involved in binding of SU(VAR)3-9 and methylation of H3K9 and H3K27 in chromosomes of Drosophila melanogaster.
abstract: In the present work, we found that the SUUR protein is required for the SU(VAR)3-9 enzyme to bind to the salivary gland polytene chromosomes. The SuUR mutation results in loss of SU(VAR)3-9 on the chromosomes, whereas artificial expression of the SuUR gene restores its binding. The SUUR protein is also involved in methylation of the residues H3K9 and H3K27. However, mono-, di-, and tri-methylated forms of H3K9 and H3K27 behave differently in various chromosomal domains in response to the SuUR mutation. Euchromatin and chromosome 4 are almost completely deprived of mono-, di-, and tri-methylation of H3K9. In the chromocenter, mono-methylation is reduced, di-methylation shows no noticeable changes, and tri-methylation is lost. Furthermore, mono- and di-methylation of H3K27 are not influenced by the SuUR mutation, whereas tri-methylation is lost in the chromocenter. Artificial expression of the SuUR gene on the SuUR (-) background restores the pattern of methylated residues characteristic for the wild type.
authors.affiliation: Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Ave., 8, Novosibirsk, 630090, Russia. koryakov@mcb.nsc.ru
authors.forename: Dmitry E
authors.initials: DE
authors.lastname: Koryakov
authors.forename: Matthias
authors.initials: M
authors.lastname: Walther
authors.forename: Anja
authors.initials: A
authors.lastname: Ebert
authors.forename: Sandro
authors.initials: S
authors.lastname: Lein
authors.forename: Igor F
authors.initials: IF
authors.lastname: Zhimulev
authors.forename: Gunter
authors.initials: G
authors.lastname: Reuter
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D011485:Protein Binding; D012469:Salivary Glands
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; C464850:SuUR protein, Drosophila; D008780:Methyltransferases; C445404:SU(VAR)3-9