title: Involvement of serine proteinase in infectious pancreatic necrosis virus capsid protein maturation and NS proteinase cleavage in CHSE-214 cells.
abstract: An investigation of virus-specific protein maturation in infectious pancreatic necrosis virus (IPNV) infected Chinook salmon embryo cells (CHSE-214) was undertaken. The precursor protein (pVP2-1) of the major mature capsid protein (VP2) was processed sequentially from pVP2-1 to pVP2-2 and VP2. Experiments using serine proteinase inhibitors showed that the maturation of the VP2 was blocked in the pVP2-1 post-translational cleavage steps. A protinin, a potent proteinase inhibitor, at 800 μg ml(-1) blocked pVP2-2 to VP2 and the cleavage of VP4 (28 kDa) to VP4-1 (25 kDa). Therefore, our data showed that the maturation of the capsid protein (VP2) and cleavage of VP4 (NS proteinase) can be blocked by serine proteinase inhibitors.
authors.affiliation: 1 Molecular Biology Laboratory, Institute of Zoology, Academia Sinica, Nankang, Taipei, Taiwan, 2 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
authors.forename: J L
authors.initials: JL
authors.lastname: Wu
authors.forename: J R
authors.initials: JR
authors.lastname: Hong
authors.forename: C Y
authors.initials: CY
authors.lastname: Chang
authors.forename: C F
authors.initials: CF
authors.lastname: Hui
authors.forename: C F
authors.initials: CF
authors.lastname: Liao
authors.forename: Y L
authors.initials: YL
authors.lastname: Hsu