title: Related B cell clones that populate the CSF and CNS of patients with multiple sclerosis produce CSF immunoglobulin.
abstract: We investigated the overlap shared between the immunoglobulin (Ig) proteome of the cerebrospinal fluid (CSF) and the B cell Ig-transcriptome of CSF and the central nervous system (CNS) tissue of three patients with multiple sclerosis. We determined the IgG-proteomes of CSF by mass spectrometry, and compared them to the IgG-transcriptomes from CSF and brain lesions, which were analyzed by cDNA cloning. Characteristic peptides that were identified in the CSF-proteome could also be detected in the transcriptomes of both, brain lesions and CSF, providing evidence for a strong overlap of the IgG repertoires in brain lesions and in the CSF.
authors.affiliation: Institute of Clinical Neuroimmunology, Ludwig Maximilians University, 81377 Munich, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Obermeier
authors.forename: Laura
authors.initials: L
authors.lastname: Lovato
authors.forename: Reinhard
authors.initials: R
authors.lastname: Mentele
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brück
authors.forename: Ignasi
authors.initials: I
authors.lastname: Forne
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.forename: Friedrich
authors.initials: F
authors.lastname: Lottspeich
authors.forename: Katherine W
authors.initials: KW
authors.lastname: Turk
authors.forename: Simon N
authors.initials: SN
authors.lastname: Willis
authors.forename: Hartmut
authors.initials: H
authors.lastname: Wekerle
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hohlfeld
authors.forename: David A
authors.initials: DA
authors.lastname: Hafler
authors.forename: Kevin C
authors.initials: KC
authors.lastname: O'Connor
authors.forename: Klaus
authors.initials: K
authors.lastname: Dornmair
mesh_terms: D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000134:cerebrospinal fluid*; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000235:genetics / Q000276:immunology*
chemical_list: D007136:Immunoglobulins