title: A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.
abstract: Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.
authors.affiliation: Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands. g.voets@umcutrecht.nl
authors.forename: Guido M
authors.initials: GM
authors.lastname: Voets
authors.forename: A C
authors.initials: AC
authors.lastname: Fluit
authors.forename: Jelle
authors.initials: J
authors.lastname: Scharringa
authors.forename: James
authors.initials: J
authors.lastname: Cohen Stuart
authors.forename: Maurine A
authors.initials: MA
authors.lastname: Leverstein-van Hall
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D005838:Genotype; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D001618:beta-Lactamases; C063912:carbapenemase