title: A glucose biosensor based on chitosan-Prussian blue-multiwall carbon nanotubes-hollow PtCo nanochains formed by one-step electrodeposition.
abstract: In this paper, a simple one-step electrodeposition method is described to fabricate chitosan-Prussian blue-multiwall carbon nanotubes-hollow PtCo nanochains (CS-PB-MWNTs-H-PtCo) film onto the gold electrode surface, then glucose oxidase (GOD) and Nafion were modified onto the film subsequently to fabricate a glucose biosensor. The morphologies and electrochemistry of the composite were investigated by using Fourier transform infrared (FTIR) spectrometry, scanning electron microscopy (SEM) and electrochemical techniques including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), respectively. The performances of the biosensor have been investigated by chronoamperometry method under the optimized conditions. This biosensor showed a linear response to glucose range from 1.5 μM to 1.12 mM with a detection limit of 0.47 μM (S/N=3), a high sensitivity of 23.4 μA mM(-1) cm(-2), and a fast response time. The apparent Michaelis-Menten constant (K(M)(app)) was 1.89 mM. In addition, the biosensor also exhibited strong anti-interference ability, excellent stability and good reproducibility.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China. chexingg-613@163.com
authors.forename: Xin
authors.initials: X
authors.lastname: Che
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Zhongju
authors.initials: Z
authors.lastname: Song
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Zhong
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005295:Ferrocyanides; D037742:Nanotubes, Carbon; D003035:Cobalt; D010984:Platinum; D006046:Gold; D048271:Chitosan; D005947:Glucose; C000170:ferric ferrocyanide