title: Electrochemiluminescence immunosensor for α-fetoprotein using Ru(bpy)3(2+)-encapsulated liposome as labels.
abstract: In this work, an electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of α-fetoprotein (AFP) was fabricated using Ru(bpy)(3)(2+)-encapsulated liposome as the label and electrodeposited gold nanoparticles (GNPs) as the immobilizing support. Great signal amplification was achieved since liposome could encapsulate large amount of reporter molecules and GNPs could provide large active surface. Under optimized conditions, with sandwich type format, a linear range of AFP from 0.005 to 0.2 pg/mL and an extremely low detection limit of 0.001 pg/mL was obtained, much lower than that in previous reports. The proposed ECL immnuosensor showed high sensitivity, specificity, and good stability, which may open a new door to ultrasensitive detection of proteins in clinical analysis.
authors.affiliation: Anhui Key Laboratory of Chemo-biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. hywang@mail.ahnu.edu.cn
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Dongyan
authors.initials: D
authors.lastname: Sun
authors.forename: Zhian
authors.initials: Z
authors.lastname: Tan
authors.forename: Wu
authors.initials: W
authors.lastname: Gong
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D015374:Biosensing Techniques; D002214:Capsules; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008081:Liposomes / Q000737:chemistry*; D008163:Luminescent Measurements; D053758:Nanoparticles / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D002214:Capsules; D008081:Liposomes; D009942:Organometallic Compounds; D000509:alpha-Fetoproteins; C469719:ruthenium (bpy)3(2+)-C2O4(2-); D015082:2,2'-Dipyridyl; D006046:Gold