title: Tannase enzyme production by entrapped cells of Aspergillus niger FETL FT3 in submerged culture system.
abstract: The ability of immobilized cell cultures of Aspergillus niger FETL FT3 to produce extracellular tannase was investigated. The production of enzyme was increased by entrapping the fungus in scouring mesh cubes compared to free cells. Using optimized parameters of six scouring mesh cubes and inoculum size of 1 × 10(6) spores/mL, the tannase production of 3.98 U/mL was obtained from the immobilized cells compared to free cells (2.81 U/mL). It was about 41.64% increment. The immobilized cultures exhibited significant tannase production stability of two repeated runs.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Darah
authors.forename: G
authors.initials: G
authors.lastname: Sumathi
authors.forename: K
authors.initials: K
authors.lastname: Jain
authors.forename: S H
authors.initials: SH
authors.lastname: Lim
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development* / Q000648:ultrastructure; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002452:Cell Count; D018914:Cells, Immobilized / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004795:Enzyme Stability
chemical_list: D003470:Culture Media; D002265:Carboxylic Ester Hydrolases; C022711:tannase