title: Ranunculus latent virus: a strain of artichoke latent virus or a new macluravirus infecting artichoke?
abstract: An elongated virus was isolated from artichoke crops in Liguria, and a 700-bp fragment was amplified by RT-PCR using oligonucleotides to detect members of the family Potyviridae. Comparison of fragment sequences showed 98% identity at the nucleotide level with the ranunculus isolate of the macluravirus Ranunculus latent virus (RaLV). RaLV was then detected by DAS-ELISA in symptomatic and asymptomatic artichoke plants from Liguria, Sardinia and Latium. The sequence of a 5.5-kb region was assembled from a cDNA library, and a 500-bp NIa fragment showed 80% identity to Artichoke latent virus.
authors.affiliation: Istituto di Virologia Vegetale, Sez. di Torino, CNR, Strada delle Cacce n73, Torino 10135, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ciuffo
authors.forename: M
authors.initials: M
authors.lastname: Testa
authors.forename: R
authors.initials: R
authors.lastname: Lenzi
authors.forename: M
authors.initials: M
authors.lastname: Turina
mesh_terms: D001483:Base Sequence; D031943:Cynara scolymus / Q000821:virology*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D019176:Potyviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D031947:Ranunculus / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D012367:RNA, Viral