title: Sensitive and rapid detection of Alicyclobacillus acidoterrestris using loop-mediated isothermal amplification.
abstract: BACKGROUND
  A loop-mediated isothermal amplification (LAMP) assay was developed for the rapid detection (within 2 h) of Alicyclobacillus acidoterrestris. The assay detected the species-specific DNA sequence of the 16S-23S rRNA internal transcribed spacer.
  
  
  RESULTS
  The eight strains of A. acidoterrestris were successfully amplified, but six strains of other bacillus Acidocaldarius and 13 bacterial species other than bacillus Acidocaldarius were not. The sensitivity of the LAMP assay was at 4.50 × 10(-2) cfu per tube. This sensitivity is greater than that obtained by polymerase chain reaction (PCR) assay. The LAMP assay was examined further for its ability to detect A. acidoterrestris in juice samples. The results were compared with those of conventional PCR detection.
  
  
  CONCLUSIONS
  Results indicate that the proposed LAMP assay is a rapid, specific and sensitive method for detecting A. acidoterrestris. As the amplification has been conducted under isothermal conditions, only a water bath or heating block is needed to maintain the required temperature. Thus, the method can be generalised and popularised easily in the future.
authors.affiliation: College of Food Science and Technology, Agricultural University of Hebei, Baoding City, Hebei province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Ma
authors.forename: Yaowu
authors.initials: Y
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D056450:Alicyclobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001628:Beverages / Q000382:microbiology; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005516:Food Microbiology; D005798:Genes, Bacterial; D021141:Nucleic Acid Amplification Techniques; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S