title: Identification of ABCA1 and ABCG1 in milk fat globules and mammary cells--implications for milk cholesterol secretion.
abstract: The ATP-binding cassette (ABC) transporters ABCA1 and ABCG1 play an important role in cellular cholesterol homeostasis, but their function in mammary gland (MG) tissue remains elusive. A bovine MG model that allows repeated MG sampling in identical animals at different functional stages was used to test whether 1) ABCA1 and ABCG1 protein expression and subcellular localization in mammary epithelial cells (MEC) change during the pregnancy-lactation cycle, and 2) these 2 proteins were present in milk fat globules (MFG). Expression and localization in MEC were investigated in bovine MG tissues at the end of lactation, during the dry period (DP), and early lactation using immunohistochemical and immunofluorescence approaches. The presence of ABCA1 and ABCG1 in MFG isolated from fresh milk was determined by immunofluorescence. The ABCA1 protein expression in MEC, expressed as arbitrary units, was higher during the end of lactation (12.2±0.24) and the DP (12.5±0.22) as compared with during early lactation (10.2±0.65). In contrast, no significant change in ABCG1 expression existed between the stages. Throughout the cycle, ABCA1 and ABCG1 were detected in the apical (41.9±24.8 and 49.0±4.96% of cows, respectively), basal (56.2±28.1 and 54.6±7.78% of cows, respectively), or entire cytoplasm (56.8±13.4 and 61.6±14.4% of cows, respectively) of MEC, or showed combined localization. Unlike ABCG1, ABCA1 was absent at the apical aspect of MEC during early lactation. Immunolabeling experiments revealed the presence of ABCA1 and ABCG1 in MFG membranes. Findings suggest a differential, functional stage-dependent role of ABCA1 and ABCG1 in cholesterol homeostasis of the MG epithelium. The presence of ABCA1 and ABCG1 in MFG membranes suggests that these proteins are involved in cholesterol exchange between MEC and alveolar milk.
authors.affiliation: Institute of Biochemistry and Molecular Medicine, University of Bern, CH-3012 Bern, Switzerland.
authors.forename: O
authors.initials: O
authors.lastname: Mani
authors.forename: M
authors.initials: M
authors.lastname: Körner
authors.forename: C E
authors.initials: CE
authors.lastname: Ontsouka
authors.forename: M T
authors.initials: MT
authors.lastname: Sorensen
authors.forename: K
authors.initials: K
authors.lastname: Sejrsen
authors.forename: R M
authors.initials: RM
authors.lastname: Bruckmaier
authors.forename: C
authors.initials: C
authors.lastname: Albrecht
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000032:analysis*; D000818:Animals; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D007774:Lactation / Q000502:physiology; D066292:Lipid Droplets; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy
chemical_list: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D006017:Glycolipids; D006023:Glycoproteins; C110367:milk fat globule; D002784:Cholesterol