title: Oligodendrocyte differentiation and myelination defects in OMgp null mice.
abstract: OMgp is selectively expressed in CNS by oligodendrocyte. However, its potential role(s) in oligodendrocyte development and myelination remain unclear. We show that OMgp null mice are hypomyelinated in their spinal cords, resulting in slower ascending and descending conduction velocities compared to wild-type mice. Consistent with the hypomyelination, in the MOG induced EAE model, OMgp null mice show a more severe EAE clinical disease and slower nerve conduction velocity compared to WT animals. The contribution of OMgp to oligodendrocyte differentiation and myelination was verified using cultured oligodendrocytes from null mice. Oligodendrocytes isolated from OMgp null mice show a significant decrease in the number of MBP(+) cells and in myelination compared to wild-type mice. The dramatic effects of the OMgp KO in oligodendrocyte maturation in vivo and in vitro reveal a new and important function for OMgp in regulating CNS myelination.
authors.affiliation: Biogen Idec Inc, 14 Cambridge Center, Cambridge, MA 02142, USA.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lee
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Hu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhongshu
authors.initials: Z
authors.lastname: Yang
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Shao
authors.forename: Mengsheng
authors.initials: M
authors.lastname: Qiu
authors.forename: Blake
authors.initials: B
authors.lastname: Pepinsky
authors.forename: Robert H
authors.initials: RH
authors.lastname: Miller
authors.forename: Sha
authors.initials: S
authors.lastname: Mi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000503:physiopathology; D058851:GPI-Linked Proteins; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009185:Myelin Proteins; D009186:Myelin Sheath / Q000378:metabolism / Q000473:pathology*; D018992:Myelin-Associated Glycoprotein / Q000235:genetics* / Q000378:metabolism; D063308:Myelin-Oligodendrocyte Glycoprotein; D009431:Neural Conduction / Q000502:physiology; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*
chemical_list: D058851:GPI-Linked Proteins; C571326:MOG protein, human; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; C560528:OMG protein, human; C560529:Omg protein, mouse