title: EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD.
abstract: OBJECTIVE
  Extracellular matrix metalloproteinase inducer (EMMPRIN or CD147) induces the production of matrix metalloproteinases (MMP) such as MMP-9, which plays an important role in COPD. We determined its cellular origin and role in MMP-9 production in COPD.
  
  
  METHODS
  Bronchial biopsies, alveolar macrophages (AM) and blood monocytes (BM) from patients with COPD, healthy smokers and non-smokers, and bronchial epithelial cells (EC) from surgically resected airways from patients with COPD were stimulated with LPS or CRP in the presence and absence of an anti-EMMPRIN blocking antibody. EMMPRIN in BAL, plasma, conditioned media and cell lysates was quantified and immunohistochemical localization of EMMPRIN was determined in bronchial biopsies. MMP-9 activity and mRNA was also determined.
  
  
  RESULTS
  EMMPRIN levels in BAL fluid were higher in patients with COPD compared with non-smokers and smokers. There was greater EMMPRIN expression in EC from patients with COPD compared with smokers and non-smokers. EC secreted and expressed more EMMPRIN protein than BM and AM. Blocking EMMPRIN decreased MMP-9 activity in supernatant of EC, but not in those from AM and BM, and decreased MMP-9 mRNA expression in EC.
  
  
  CONCLUSIONS
  The increased EMMPRIN expression in COPD is reflected by an increased release from bronchial EC, which are one of the main source of EMMPRIN. EMMPRIN regulates MMP-9 expression in COPD.
authors.affiliation: Experimental Studies, Airway Disease Section, National Heart and Lung Institute, Imperial College London Royal Brompton Hospital, London, UK.
authors.forename: Stephane
authors.initials: S
authors.lastname: Jouneau
authors.forename: Nadia
authors.initials: N
authors.lastname: Khorasani
authors.forename: Patricia
authors.initials: P
authors.lastname: DE Souza
authors.forename: Patricia
authors.initials: P
authors.lastname: Macedo
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.forename: Pankaj K
authors.initials: PK
authors.lastname: Bhavsar
authors.forename: Kian F
authors.initials: KF
authors.lastname: Chung
mesh_terms: D000293:Adolescent; D000328:Adult; D019138:Antibodies, Blocking / Q000494:pharmacology; D051926:Basigin / Q000378:metabolism*; D001706:Biopsy; D001980:Bronchi / Q000378:metabolism*; D002097:C-Reactive Protein / Q000494:pharmacology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000601:surgery; D012907:Smoking / Q000378:metabolism; D055815:Young Adult
chemical_list: D019138:Antibodies, Blocking; D008070:Lipopolysaccharides; D051926:Basigin; D002097:C-Reactive Protein; D020780:Matrix Metalloproteinase 9