title: Development and validation of HPLC-UV-MS method for the control of four anti-diabetic drugs in suspected counterfeit products.
abstract: An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.0) at a wavelength of 210 nm. Furthermore, the method of a high-performance liquid chromatography coupled with ESI-MS in positive ionization mode has been established. These two methods were successfully applied to the assay and qualitative detection of four alpha-glucosidase inhibitors in the potential counterfeit anti-diabetic drugs.
authors.affiliation: National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Dai
authors.forename: Ning
authors.initials: N
authors.lastname: An
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Wu
authors.forename: Hui-yi
authors.initials: HY
authors.lastname: Li
authors.forename: Qi-ming
authors.initials: QM
authors.lastname: Zhang
mesh_terms: D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives* / Q000032:analysis; D020909:Acarbose / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000737:chemistry; D007294:Inositol / Q000031:analogs & derivatives* / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D000520:alpha-Glucosidases / Q000032:analysis*
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; C045621:miglitol; D017485:1-Deoxynojirimycin; D007294:Inositol; D000520:alpha-Glucosidases; C102817:voglibose; D020909:Acarbose