title: The Polycomb group protein CRAMPED is involved with TRF2 in the activation of the histone H1 gene.
abstract: CRAMPED (CRM), conserved from plants to animals, was previously characterized genetically as a repressive factor involved in the formation of facultative and constitutive heterochromatin (Polycomb silencing, position effect variegation). We show that crm is dynamically regulated during replication and identify the Histone gene cluster (His-C) as a major CRM target. Surprisingly, CRM is specifically required for the expression of the Histone H1 gene, like the promoter-bound transcription factor TRF2. Consistently with this, CRM genetically interacts and co-immunoprecipitates with TRF2. However, the Polycomb phenotypes observed in crm mutants are not observed in TRF2 hypomorphic mutants, suggesting that they correspond to independent roles of CRM. CRM is thus a highly pleiotropic factor involved in both activation and repression.
authors.affiliation: Department of Zoology and Animal Biology, University of Geneva, Switzerland. Jean-Michel.Gibert@unige.ch
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Gibert
authors.forename: François
authors.initials: F
authors.lastname: Karch
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D006570:Heterochromatin / Q000235:genetics; D006657:Histones / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D063146:Polycomb-Group Proteins; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism; D035341:Telomeric Repeat Binding Protein 2 / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D029721:Drosophila Proteins; D006570:Heterochromatin; D006657:Histones; D018398:Homeodomain Proteins; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; C509085:TRF2 protein, Drosophila; D035341:Telomeric Repeat Binding Protein 2; D014157:Transcription Factors; C108530:crm protein, Drosophila