title: Wheat vacuolar H+-ATPase subunit B cloning and its involvement in salt tolerance.
abstract: Wheat vacuolar H+-ATPases (V-ATPase) subunit B, named TaVB, was isolated from the salt-tolerant wheat RH8706-49 and used to transform Arabidopsis plants. TaVB-expressed Arabidopsis has a higher germination rate, root length, V-H+-ATPase activity, and overall salt tolerance than the wild type, indicating that expression of the gene can affect salt tolerance of the transgenic plants. Under salt stress, intracellular Na+ levels in transgenic plants are significantly lower than the control. These results suggest that expression of the wheat TaVB gene may enhance plant tolerance to salt stress.
authors.affiliation: College of Life Science of Hebei Normal University, No. 113 Yuhua East Road, Shijiazhuang, Hebei, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: He
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yinzhu
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhanjing
authors.initials: Z
authors.lastname: Huang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000378:metabolism; D014908:Triticum / Q000201:enzymology* / Q000235:genetics; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
chemical_list: D012965:Sodium Chloride; D025262:Vacuolar Proton-Translocating ATPases