title: Knockdown of DAPIT (diabetes-associated protein in insulin-sensitive tissue) results in loss of ATP synthase in mitochondria.
abstract: It was found recently that a diabetes-associated protein in insulin-sensitive tissue (DAPIT) is associated with mitochondrial ATP synthase. Here, we report that the suppressed expression of DAPIT in DAPIT-knockdown HeLa cells causes loss of the population of ATP synthase in mitochondria. Consequently, DAPIT-knockdown cells show smaller mitochondrial ATP synthesis activity, slower growth in normal medium, and poorer viability in glucose-free medium than the control cells. The mRNA levels of α- and β-subunits of ATP synthase remain unchanged by DAPIT knockdown. These results indicate a critical role of DAPIT in maintaining the ATP synthase population in mitochondria and raise an intriguing possibility of active role of DAPIT in cellular energy metabolism.
authors.affiliation: International Cooperative Research Project ATP-Synthesis Regulation Project, Japan Science and Technology Agency, 2-3-6 Aomi, Koto-Ku, Tokyo 135-0064, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Ohsakaya
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujikawa
authors.forename: Toru
authors.initials: T
authors.lastname: Hisabori
authors.forename: Masasuke
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D004734:Energy Metabolism / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: C000606721:ATP5MK protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D025261:Mitochondrial Proton-Translocating ATPases