title: Preparation and Use of Microcolumns for Sample Desalting or Nanoscale IMAC.
abstract: INTRODUCTIONIn this method, microcolumns are prepared using any kind of chromatographic resin. The resin is held in place by making a constriction at the end of a GELoader pipette tip. Sample loading, washing, and elution are performed by loading liquid on top of the resin and applying air pressure to generate a low flow through the column. No frits are necessary.
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.forename: Allan
authors.initials: A
authors.lastname: Stensballe
authors.forename: Ole N
authors.initials: ON
authors.lastname: Jensen