title: Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis.
abstract: In this study, we investigated a novel application of matrix solid-phase dispersion (MSPD) methodology for the extraction of endogenous peptides from porcine hypothalamus tissue samples. Several experimental factors of the MSPD procedure were examined. Finally, silica-based octadecyl was chosen as dispersing material and blended with 0.25 g porcine hypothalamus at a ratio of 5, and 10 mL of 60% acetonitrile with 0.2% formic acid in water was chosen as the extraction and elution solvent. This MSPD extraction method was compared to the classic acid extraction method. More peaks were observed in the MSPD extracts (74±5) by MALDI-TOF MS than in acid extracts (34±5). Moreover, 14 potential endogenous peptides were identified in the MSPD extracts after nanoLC-MS/MS analysis, while only 2 endogenous peptides in the acid extracts. These results indicated that MSPD could be employed as a simple and efficient method for the extraction of endogenous peptides from tissues.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, Liaoning, PR China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Cai
authors.forename: Chaoran
authors.initials: C
authors.lastname: Wang
authors.forename: Junyan
authors.initials: J
authors.lastname: Xu
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000097:Acetonitriles; D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005561:Formates; D007031:Hypothalamus / Q000737:chemistry*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013552:Swine
chemical_list: D000097:Acetonitriles; D005561:Formates; D009419:Nerve Tissue Proteins; D010455:Peptides; C030544:formic acid; C032159:acetonitrile