title: Latent membrane protein 1 of Epstein-Barr virus regulates death-associated protein kinase 1 in lymphoblastoid cell line.
abstract: The Epstein-Barr virus (EBV) infects and transforms primary B cells into lymphoblastoid cell lines (LCLs). We observed death-associated protein kinase 1 (DAPK1) upregulation in B cells following EBV infection and high DAPK1 levels in LCLs. DAPK1 participates in several apoptosis-inducing pathways, yet DAPK1 expression increased during B cell transformation. Data from LMP1 overexpression in LCLs and HeLa cells and from knocked down LMP1 in LCLs suggest LMP1 regulation of DAPK1 expression. We observed NF-κB signaling in DAPK1 upregulation by LMP1 with CTAR deletion mutants failing to induce DAPK1 expression and with Bay11 blocking DAPK1 expression. DAPK1 is inactive in LCLs due to insufficient stimuli, and is not regulated by Ser308 phosphorylation. However, DAPK1 in LCLs is functional, as evidenced by its quick mediation of cell death following UV or H(2)O(2) exposure, and increased survival among LCLs knocked down with DAPK. DAPK roles in EBV-infected B cells remain to be identified.
authors.affiliation: Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chun-Wei
authors.initials: CW
authors.lastname: Lee
authors.forename: Shr-Jeng Jim
authors.initials: SJ
authors.lastname: Leu
authors.forename: Ruei-Ying
authors.initials: RY
authors.lastname: Tzeng
authors.forename: Sheng-Fan
authors.initials: SF
authors.lastname: Wang
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Tsai
authors.forename: Kuang-Hui
authors.initials: KH
authors.lastname: Sun
authors.forename: Ruey-Hwa
authors.initials: RH
authors.lastname: Chen
authors.forename: Jason C
authors.initials: JC
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000821:virology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D064550:Death-Associated Protein Kinases; D020031:Epstein-Barr Virus Infections / Q000201:enzymology* / Q000503:physiopathology / Q000821:virology; D015971:Gene Expression Regulation, Enzymologic; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; C579096:DAPK1 protein, human; D064550:Death-Associated Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases