title: Phosphorous deficiency decreases nitrogenase activity but increases proton efflux in N2-fixing Medicago truncatula.
abstract: Effects of Sinorhizobium strain and P nutrition on N(2)-dependent growth, nitrogenase activity and proton efflux by nodulated roots were investigated in the model legume Medicago truncatula cultivar Jemalong grown in hydroaeroponic culture in symbioses with Sinorhizobium meliloti strains 102F51 and 2011. Sinorhizobium strain had strong effects on nitrogenase activity and N(2)-dependent growth, with S. meliloti 102F51 being the more efficient strain. Apparent and total nitrogenase activities, measured by hydrogen evolution in air and argon, respectively, were drastically reduced in plants supplied with 5 μmol P plant(-1) week(-1) as compared with 15 μmol P plant(-1) week(-1). There was a net proton efflux as soon as 2 weeks after inoculation and, in contrast to the effect of P nutrition on nitrogenase activity, P deficiency increased total and specific proton effluxes, irrespective of Sinorhizobium strain.
authors.affiliation: Department für Nutzpflanzenwissenschaften Abteilung Pflanzenernährung, Georg-August-Universität Göttingen, Göttingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schulze
authors.forename: M A N
authors.initials: MA
authors.lastname: Mohamed
authors.forename: G
authors.initials: G
authors.lastname: Carlsson
authors.forename: J J
authors.initials: JJ
authors.lastname: Drevon
mesh_terms: D000383:Agriculture / Q000379:methods; D001692:Biological Transport; D006859:Hydrogen / Q000378:metabolism; D046913:Medicago truncatula / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D011522:Protons; D053204:Root Nodules, Plant / Q000378:metabolism* / Q000382:microbiology; D016962:Sinorhizobium meliloti / Q000145:classification; D013045:Species Specificity; D013559:Symbiosis
chemical_list: D011522:Protons; D010758:Phosphorus; D006859:Hydrogen; D009591:Nitrogenase