title: Hepatoprotective activity of Luffa acutangula against CCl4 and rifampicin induced liver toxicity in rats: a biochemical and histopathological evaluation.
abstract: Hepatoprotective activity of hydroalcoholic extract of Luffa acutangula (HAELA) against carbon tetrachloride (CCl4) and rifampicin-induced hepatotoxicity in rats was evaluated and probable mechanism(s) of action has been suggested. Administration of standard drug- silymarin and HAELA showed significant hepatoprotection against CCl4 and rifampicin induced hepatotoxicity in rats. Hepatoprotective activity of HAELA was due to the decreased levels of serum marker enzymes viz., (AST, ALT, ALP and LDH) and increased total protein including the improvement in histoarchitecture of liver cells of the treated groups as compared to the control group. HAELA also showed significant decrease in malondialdehyde (MDA) formation, increased activity of non-enzymatic intracellular antioxidant, glutathione and enzymatic antioxidants, catalase and superoxide dismutase. Results of this study demonstrated that endogenous antioxidants and inhibition of lipid peroxidation of membrane contribute to hepatoprotective activity of HAELA.
authors.affiliation: Department of Pharmacology, Padm Dr D Y Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune 411 018, India.
authors.forename: Vishal B
authors.initials: VB
authors.lastname: Jadhav
authors.forename: Vishnu N
authors.initials: VN
authors.lastname: Thakare
authors.forename: Anupama A
authors.initials: AA
authors.lastname: Suralkar
authors.forename: Avinash D
authors.initials: AD
authors.lastname: Deshpande
authors.forename: Suresh R
authors.initials: SR
authors.lastname: Naik
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D029776:Luffa / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012293:Rifampin / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D012293:Rifampin