title: [Construction of genetic linkage map of Bupleurum chinense DC. using ISSR and SSR markers].
abstract: Molecular genetic map is a fundamental organizational tool for genomic research. However, a genetic linkage map for Bupleurum chinense DC. has not been developed. In this study, with the theory of pseudo-testcross, 96 F1 plants from an intraspecific cross of B. chinense were used as mapping populations. Twenty eight ISSR (inter-simple sequence repeat) primers and 44 SSR (simple sequence repeat) primers were used to detect the polymorphisms between the parental plants, and of them, 28 ISSRs and 14 SSRs were selected to analyze the F1 populations. The map consisted of 13 linkage groups which included 80 (72 ISSRs and 8 SSRs) loci, and covered 2 633.9 cM with an average density of 33.4 cM. All 13 linkage groups consisted of 2-31 loci ranging in length from 15.4-1295.7 cM. This map will provide a basis for studies on gene mapping, map-based cloning and maker-assisted selection of important traits in B. chinense.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Zhan
authors.forename: Chun
authors.initials: C
authors.lastname: Sui
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Wei
authors.forename: Sheng-Ci
authors.initials: SC
authors.lastname: Fan
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D029972:Bupleurum / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D018895:Microsatellite Repeats; D010946:Plants, Medicinal / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant