title: Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema.
abstract: A better understanding of the underlying mechanisms of angiogenesis and vascular permeability is necessary for the development of therapeutic strategies for ischemic injury. The purpose of this study was to examine the spatial and temporal expression of Src and Src-suppressed C kinase substrate (SSeCKS) in brain after middle cerebral artery occlusion (MCAO) and elucidate the relationships among Src, SSeCKS, and the key angiogenic factors present after stroke. Rats were subjected to either MCAO or sham operation. Reverse transcriptase-polymerase chain reaction and Western blotting results revealed that Src gradually increased starting as early as 2 h after MCAO and remained high for 1 day. In contrast, SSeCKS decreased after MCAO. Src expression correlated positively with that of vascular endothelial growth factor and angiopoietin-2, and negatively with that of SSeCKS, angiopoietin-1, and zonula occludens-1. However, SSeCKS had the reverse correlations. Changes in the expression of these factors correlated with the progress of angiogenesis and cerebral edema. Dynamic temporal changes in Src and SSeCKS expression may modulate angiogenesis and cerebral edema formation after focal cerebral ischemia.
authors.affiliation: Department of Pathology, First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin 150001, PR China.
authors.forename: Likun
authors.initials: L
authors.lastname: Zan
authors.forename: He
authors.initials: H
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Shiguang
authors.initials: S
authors.lastname: Zhao
authors.forename: Yuejia
authors.initials: Y
authors.lastname: Song
authors.forename: Guoxin
authors.initials: G
authors.lastname: Teng
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Jia
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiping
authors.initials: J
authors.lastname: Qi
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D054758:A Kinase Anchor Proteins / Q000096:biosynthesis*; D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D000818:Animals; D001929:Brain Edema / Q000201:enzymology* / Q000209:etiology; D002545:Brain Ischemia / Q000150:complications / Q000201:enzymology*; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D008297:Male; D009389:Neovascularization, Pathologic / Q000201:enzymology* / Q000209:etiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019061:src-Family Kinases / Q000096:biosynthesis*
chemical_list: D054758:A Kinase Anchor Proteins; C102361:Akap12 protein, rat; D043925:Angiogenesis Inducing Agents; D018797:Cell Cycle Proteins; C490511:Frk protein, rat; D019061:src-Family Kinases