title: Effect of different resistance-training regimens on the WNT-signaling pathway.
abstract: The purpose of the present study was to evaluate the effects of 8 weeks of strength and power training on the expression of genes related to the canonical WNT pathway and β-catenin protein levels in physically active men. Twenty-five subjects (27.4 ± 4.6 years) were balanced based on their relative maximum strength in the squat exercise (squat 1RM/body mass) and randomly assigned to strength training (ST) (n = 10), power training (PT) (n = 10), and control (C) (n = 5) groups. The ST and the PT groups performed high and low intensity squats, respectively, thrice a week, for 8 weeks. Muscle biopsies from the vastus lateralis muscle were collected before and after the training period. Relative strength and power increased similarly in both ST and PT groups (P < 0.001). Fiber cross-sectional area also increased similarly in both ST and PT groups. Gene expression and β-catenin protein expression levels were assessed by real-time PCR and Western blot. Certain genes were up-regulated in the ST group (WNT1: 6.4-fold, P < 0.0001; SFRP1: 3.3-fold, P < 0.0001 and LEF1: 7.3-fold, P < 0.0001) and also in the PT group (WNT1: 24.9-fold, P < 0.0001; SFRP1: 2.7-fold, P < 0.0001; LEF1: 34.1-fold, P < 0.0001 and Cyclin D1: 7.7-fold, P < 0.001). In addition, the expression of key WNT pathway genes was substantially more responsive to PT than to ST (WNT1: P < 0.0001; LEF1: P < 0.0001 and Cyclin D1: P < 0.001). Finally, the total β-catenin protein content increased only in the PT group (P < 0.05). Our data indicate that a PT regimen triggers greater responses in key elements of the WNT pathway.
authors.affiliation: Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1524 Butanta, São Paulo, SP, Brazil.
authors.forename: Marcelo Larciprete
authors.initials: ML
authors.lastname: Leal
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lamas
authors.forename: Marcelo Saldanha
authors.initials: MS
authors.lastname: Aoki
authors.forename: Carlos
authors.initials: C
authors.lastname: Ugrinowitsch
authors.forename: Marcela Sorelli Carneiro
authors.initials: MS
authors.lastname: Ramos
authors.forename: Valmor
authors.initials: V
authors.lastname: Tricoli
authors.forename: Anselmo Sigari
authors.initials: AS
authors.lastname: Moriscot
mesh_terms: D000293:Adolescent; D000328:Adult; D000465:Algorithms; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D053580:Muscle Strength / Q000235:genetics / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D010809:Physical Fitness / Q000502:physiology; D055070:Resistance Training / Q000009:adverse effects / Q000379:methods*; D057185:Sedentary Behavior; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology*; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D055815:Young Adult; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C495247:WNT1 protein, human; D051155:Wnt1 Protein; D051176:beta Catenin