title: [Paeonol attenuates oxygen-glucose deprivation injury and inhibits NMDA receptor activation of cultured rat hippocampal neurons].
abstract: The purpose of this study is to determine if paeonol can protect hippocampal neurons against injury due to oxygen-glucose deprivation (OGD) injury. The rat neurons were cultured in an OGD environment and the model of OGD injury was established. Paeonol and MK-801, a positive control drug, were added before deprivation. Neuron viability was measured by the reduction of MTT; glutamate was analyzed by amino acid analyzer; binding activity of NMDA receptor was evaluated by liquid scintillation counting and the expression of NMDA receptor NR1 subunit mRNA was semiquantitatively determined by RT-PCR. Compared with OGD injury group, paeonol treatment obviously increased cell survival rate and reduced the binding activity of NMDA receptors and the release of glutamate; and down-regulating the expression of NR1 subunit. These results suggest that paeonol may exhibit its protective effect against OGD injury by the action on NMDA receptor of rats.
authors.affiliation: Qilu Hospital, Shandong University, Jinan 250012, China.
authors.forename: Ning-ning
authors.initials: NN
authors.lastname: Song
authors.forename: Ji-biao
authors.initials: JB
authors.lastname: Wu
authors.forename: Xin-bing
authors.initials: XB
authors.lastname: Wei
authors.forename: Hua-shi
authors.initials: HS
authors.lastname: Guan
authors.forename: Xiu-mei
authors.initials: XM
authors.lastname: Zhang
mesh_terms: D000098:Acetophenones / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016291:Dizocilpine Maleate / Q000494:pharmacology; D005947:Glucose / Q000172:deficiency*; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D009474:Neurons / Q000166:cytology; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*
chemical_list: D000098:Acetophenones; C409466:NR1 NMDA receptor; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; C013638:paeonol; D016291:Dizocilpine Maleate; D005947:Glucose