title: Fast nonlinear spectral microscopy of in vivo human skin.
abstract: An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.
authors.forename: Arjen N
authors.initials: AN
authors.lastname: Bader
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Pena
authors.forename: C
authors.initials: C
authors.lastname: Johan van Voskuilen
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Palero
authors.forename: Frédéric
authors.initials: F
authors.lastname: Leroy
authors.forename: Anne
authors.initials: A
authors.lastname: Colonna
authors.forename: Hans C
authors.initials: HC
authors.lastname: Gerritsen