title: [Establishment of a novel chinese human lung adenocarcinoma cell line CPA-Yang3 and its real bone metastasis clone CPA-Yang3BM in immunodeficient mice].
abstract: OBJECTIVE
  The recurrence and metastasis of lung cancer is a tough problem worldwide. The aim of this study is to establish a novel Chinese lung adenocarcinoma cell line and its real bone-seeking clone sub-line for exploring the molecular mechanism of lung cancer metastasis.
  
  
  METHODS
  The cells came from the pleural effusion of a sixty-five years old female patient with lung adenocarcinoma and supraclavicular lymph node metastases. The gene expression was detected by real-time quantitative PCR. Intracardiac injection of the cells into nude mice was performed and in vivo imaging was obtained by bone scintigraphy and conventional radiography. Bone metastases were determined on bone scintigraphy and then the lesions were resected under deep anesthesia for bone metastasis cancer cell culture. The process was repeated for four cycles to obtain a real bone-seeking clone.
  
  
  RESULTS
  The tumorigenesis rate started at 4th passage in immunodeficient mice via subcutaneously and as well as later passages. Approximately 1×10⁶ cancer cells were injected into left cardiac ventricle of immunodeficient mice resulted bone metastasis sites were successfully revealed by bone scintigraphy and pathological diagnosis, the mandible (100%), scapula (33%), humerus (50%), vertebral column (50%), femur (66.7%) and accompanied invasion with other organs, the adrenal gland (17%), pulmonary (33%), liver (50%), submaxillary gland (33%) in the mice after inoculation two-three weeks. The chromosome karyotype analysis of the cells was subdiploid. Quantitative real-time PCR was used to examined and compared with SPC-A-1 lung adenocarcinoma, ESM1, VEGF-C, IL-6, IL-8, AR, SVIL, FN1 genes were overexpress. The novel cell was named CPA-Yang3. The femur metastasis cell was repeated in vivo-in vitro-in vivo with three cycles and harvested a real bone metastasis clone. It was named CPA-Yang3BM.
  
  
  CONCLUSIONS
  Tne characteristics of novel strain CPAYang3 is a highly metastasis cell line of Chinese lung adenocarcinoma and CPA-Yang3BM is a real bone-seeking clone.
authors.affiliation: Department of Nuclear Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China. yzyg@sh163.net
authors.forename: Shunfang
authors.initials: S
authors.lastname: Yang
authors.forename: Meiping
authors.initials: M
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Su
authors.forename: Lanxiang
authors.initials: L
authors.lastname: Zhao
authors.forename: Bei
authors.initials: B
authors.lastname: Lei
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
authors.forename: Jianying
authors.initials: J
authors.lastname: Lu
authors.forename: Jianding
authors.initials: J
authors.lastname: Ye
authors.forename: Wenhui
authors.initials: W
authors.lastname: Xie
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000368:Aged; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000276:immunology / Q000556:secondary*; D045744:Cell Line, Tumor / Q000166:cytology / Q000276:immunology; D002681:China; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured