title: Preparation and Microinjection of x-Rhodamine-Labeled Vimentin.
abstract: INTRODUCTIONIntermediate filaments (IF) are major cytoskeletal systems of vertebrate and many nonvertebrate cells whose expression is cell-type specific and developmentally regulated. This protocol describes the x-rhodamine labeling of one type of IF, vimentin, and a method for microinjection of the labeled vimentin into cultured cells. IF dynamics can then be examined with fluorescence microscopy.
authors.forename: Edward R
authors.initials: ER
authors.lastname: Kuczmarski
authors.forename: Robert D
authors.initials: RD
authors.lastname: Goldman