title: 1-Acyl-2-[N-(2,4-dinitrophenyl)aminopropionyl]-sn-glycero-3-phosphocholine as a chromogenic substrate for phospholipase A₂ assay.
abstract: We have developed a spectrophotometric assay for phospholipase A(2) activity using 2,4-dinitrophenyl-labeled phosphatidylcholine as substrate. The assay allows quite simple quantification of phospholipase A(2) activity by measuring the absorbance of the aqueous phase after extraction of the reaction mixture and requires neither chromatographic separation of the reaction products nor the addition of auxiliary coloring reagents.
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich st., 5/2, 220141 Minsk, Republic of Belarus. sharko@iboch.bas-net.by
authors.forename: Olga
authors.initials: O
authors.lastname: Sharko
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kisel
mesh_terms: D002863:Chromogenic Compounds / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D010713:Phosphatidylcholines / Q000737:chemistry*; D054467:Phospholipases A2 / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods*; D013379:Substrate Specificity
chemical_list: D002863:Chromogenic Compounds; D010713:Phosphatidylcholines; D054467:Phospholipases A2