title: Derp2-mutant gene vaccine inhibits airway inflammation and up-regulates Toll-like receptor 9 in an allergic asthmatic mouse model.
abstract: BACKGROUND
  A DNA vaccine encoding the whole segment of the Derp2 allergen could prevent allergic airway inflammation in a Derp2 allergen-induced allergic airway inflammation mouse model.
  
  
  OBJECTIVE
  This study investigated the effect of DNA vaccine encoding Derp2-mutant gene in which an IgE epitope was deleted on airway inflammation and the role of TLR9 in the asthmatic mouse model.
  
  
  METHODS
  A Derp2-mutant DNA vaccine was constructed. Mice were immunized, sensitized and challenged. Airway inflammation, airway hyper reactivity (AHR) and serum antibody were tested. The expression of Toll like receptor9 (TLR9) was detected with western-blot and immunehistochemistry.
  
  
  RESULTS
  We demonstrated that the Derp2-mutant-DNA induced IgG2a and inhibited IgE production, inhibited airway allergenic inflammation and AHR. Derp2-mutant-DNA vaccine induce TLR9 expression in lung tissue.
  
  
  CONCLUSIONS
  The data indicate that allergen DNA vaccine deleted IgE epitope could prevent allergenic airway inflammation, AHR, and upregulate lung TLR9 express.
authors.affiliation: Inflammation & Allergic Disease Lab, Affiliated Hospital of Lu Zhou Medical College, Sichuan province, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wang
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Luo
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Liao
authors.forename: Guoping
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001249:Asthma / Q000097:blood / Q000235:genetics* / Q000276:immunology / Q000628:therapy*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology; D004195:Disease Models, Animal; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D017384:Sequence Deletion / Q000235:genetics; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019444:Vaccines, DNA
chemical_list: D039741:Antigens, Dermatophagoides; C559662:Derp2 allergen, Dermatophagoides pteronyssinus; D018985:Epitopes, B-Lymphocyte; D007074:Immunoglobulin G; D051217:Toll-Like Receptor 9; D019444:Vaccines, DNA; D007073:Immunoglobulin E