title: Vasculitis and anaphylactoid shock in mice induced by the polysaccharide fraction secreted into culture supernatants by the fungus Candida metapsilosis.
abstract: The biological effects of Candida metapsilosis water-soluble fraction (CMWS), prepared using a completely synthesized medium, were examined to determine whether CMWS induces vasculitis similar to that seen in Kawasaki disease, and anaphylactoid shock, in mice. It was found that intraperitoneal injection of CMWS induces coronary arteritis and i.v. injection induces acute anaphylactoid shock in mice, similar to Candida albicans water-soluble fraction (CAWS)-induced arteritis and anaphylactoid shock. The mannan structure of the polysaccharide fraction was then analyzed by performing antiserum reactivity tests and nuclear magnetic resonance spectroscopy. The mannan structure was investigated because the present authors have recently found that the mannan moiety within the polysaccharide fraction might be responsible for these pathogenic activities. The structural analysis showed that the mannan structure within CMWS expresses α-mannan residues, but not β-mannan. In addition, the mannan structure of CMWS is quite similar to that of CAWS. The present findings indicate that the polysaccharide fraction from C. metapsilosis, which is mainly composed of mannan, contributes to coronary arteritis and acute shock, and that the mannan structure could be responsible for this pathogenicity.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Tada
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takano
authors.forename: Hisashi
authors.initials: H
authors.lastname: Murakami
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Nagi-Miura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced*; D000818:Animals; D001167:Arteritis / Q000139:chemically induced*; D002175:Candida; D002176:Candida albicans / Q000737:chemistry / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology; D003331:Coronary Vessels / Q000382:microbiology*; D017077:Culture Media, Conditioned / Q000737:chemistry; D008297:Male; D008351:Mannans / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D017077:Culture Media, Conditioned; D008351:Mannans; D011134:Polysaccharides