title: [Protective effect of shenfu injection on kidney against ischemia-reperfusion injury in rats].
abstract: OBJECTIVE
  To investigate the effect of Shenfu injection on ischemia-reperfusion injury of rat kidney and its mechanism.
  
  
  METHODS
  Fifty-five male SD rats were randomly divided into 5 groups: sham operation group (Sham group), Ischemia-reperfusion group (IR group), Shenfu injection (5 mL/kg) treated group (SF5 + IR group), Shenfu Injection (10 mL/kg) treated group (SF10+IR group) and Shenfu Injection (15 mL/kg) treated group (SF15+IR group). The rats were anesthetized with valebarbitone. Bilateral kidneys were exposed through midline incision. Right kidney was removed and left renal pedicels were occluded for 50 min with a traumatic mini-clamp then unclamped for 24 h. Animals in (SF5+IR group), (SF10+IR group) and (SF15+IR group) received Shenfu Injection of 5 mL/kg, 10 mL/kg and 15 mL/kg respectively through intraperitoneal injection every day for 7 days. At 24 hours after reperfusion, blood and renal tissue were taken for examination.
  
  
  RESULTS
  Ischemia-reperfusion injury rat model was established successfully. The level of serum BUN, CREA and MDA were lower in (SF5+IR) group and (SF10+IR)group and (SF15+IR) group than in IR group (P < 0.05). The level of SOD in (SF5+IR) group and (SF10+IR) group and (SF15+IR) group increased significantly than in IR group (P < 0.05). Furthermore, all of the changes were observed in a dose-dependent manner, (SF15 + IR) group demonstrated the best effect by compared with others (P < 0.05).
  
  
  CONCLUSIONS
  Shenfu injection can protect rats against acute renal ischemia-reperfusion injury by increasing SOD activity and decreasing MDA content.
authors.affiliation: Department of Pharmacy, the Affiliated Hospital of North Sichuan Medical College, Nanchong 63700, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jue
authors.initials: J
authors.lastname: Li
authors.forename: Ting-mo
authors.initials: TM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000098:blood supply*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D020011:Protective Agents / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; C492793:Shen-Fu; D008315:Malondialdehyde; D013482:Superoxide Dismutase