title: EMT and stem cell-like properties associated with miR-205 and miR-200 epigenetic silencing are early manifestations during carcinogen-induced transformation of human lung epithelial cells.
abstract: Epithelial-to-mesenchymal transition (EMT) is strongly associated with cancer progression, but its potential role during premalignant development has not been studied. Here, we show that a 4-week exposure of immortalized human bronchial epithelial cells (HBEC) to tobacco carcinogens can induce a persistent, irreversible, and multifaceted dedifferentiation program marked by EMT and the emergence of stem cell-like properties. EMT induction was epigenetically driven, initially by chromatin remodeling through H3K27me3 enrichment and later by ensuing DNA methylation to sustain silencing of tumor-suppressive microRNAs (miRNA), miR-200b, miR-200c, and miR-205, which were implicated in the dedifferentiation program in HBECs and also in primary lung tumors. Carcinogen-treated HBECs acquired stem cell-like features characterized by their ability to form spheroids with branching tubules and enrichment of the CD44(high)/CD24(low), CD133, and ALDH1 stem cell-like markers. miRNA overexpression studies indicated that regulation of the EMT, stem-like, and transformed phenotypes in HBECs were distinct events. Our findings extend present concepts of how EMT participates in cancer pathophysiology by showing that EMT induction can participate in cancer initiation to promote the clonal expansion of premalignant lung epithelial cells.
authors.affiliation: Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA.
authors.forename: Carmen S
authors.initials: CS
authors.lastname: Tellez
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Juri
authors.forename: Kieu
authors.initials: K
authors.lastname: Do
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Bernauer
authors.forename: Cindy L
authors.initials: CL
authors.lastname: Thomas
authors.forename: Leah A
authors.initials: LA
authors.lastname: Damiani
authors.forename: Mathewos
authors.initials: M
authors.lastname: Tessema
authors.forename: Shuguang
authors.initials: S
authors.lastname: Leng
authors.forename: Steven A
authors.initials: SA
authors.lastname: Belinsky
mesh_terms: D002273:Carcinogens; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics* / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000473:pathology; D014026:Nicotiana / Q000737:chemistry
chemical_list: D002273:Carcinogens; D035683:MicroRNAs; C533896:MIRN200 microRNA, human; C534425:MIR205, human