title: Evolution of canine parvovirus in Argentina between years 2003 and 2010: CPV2c has become the predominant variant affecting the domestic dog population.
abstract: The current frequency of Canine Parvovirus variants (CPV2a, CPV2b and CPV2c) in the Argentine dog population was investigated by PCR amplification of a 583 bp fragment in the VP2 gene. From a total of 79 rectal swab samples that have been submitted to our laboratory since 2008, 55 (69.6%) resulted positive and were further analyzed by direct DNA sequencing. Fifty positives samples (91%) were characterized as CPV2c variant, which appeared in Argentina in the year 2003 and has been the prevalent type since 2008, whereas CPV2a and CPV2b, still found in Argentine dogs, were represented in 3.6% and 5.4% of the population, respectively. Considering that CPV2c is spreading worldwide, and that this variant is also affecting vaccinated dogs, efforts should be made towards the development of new matched CPV vaccines.
authors.affiliation: Centro de Virología Animal, Instituto de Ciencia y Tecnología Dr. Cesar Milstein, CONICET, Saladillo 2468, C1440FFX, Buenos Aires, Argentina.
authors.forename: Marina Gallo
authors.initials: MG
authors.lastname: Calderón
authors.forename: Carina
authors.initials: C
authors.lastname: Romanutti
authors.forename: Alejandra
authors.initials: A
authors.lastname: D' Antuono
authors.forename: Leticia
authors.initials: L
authors.lastname: Keller
authors.forename: Nora
authors.initials: N
authors.lastname: Mattion
authors.forename: Jose
authors.initials: J
authors.lastname: La Torre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D005075:Biological Evolution; D004279:DNA, Viral / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology*; D004285:Dogs; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral