title: Gene expression changes in a rat model of oxygen-induced retinopathy.
abstract: OBJECTIVE
  To identify altered patterns of retinal mRNA expression in a rat model of oxygen-induced retinopathy (OIR).
  
  
  METHODS
  Sprague-Dawley rats from P2 to P14 were exposed to hyperoxia (80% oxygen) to induce OIR and then returned to normoxic conditions. Control rats were sustained in room air. Retinal gene expression between the rats of OIR and the controls was compared using cDNA microarray analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.
  
  
  RESULTS
  Among a total of 12,731 cDNAs analyzed by microarray, 13 genes were strongly up- or down-regulated (>2-fold change over controls) in the OIR rats. We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.
  
  
  CONCLUSIONS
  We confirmed changes in expressions of various retinal genes in a rat model of OIR by microarray and RT-PCR. This study should contribute to the understanding of genetic indicators associated with OIR.
authors.affiliation: Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea.
authors.forename: Na Eun
authors.initials: NE
authors.lastname: Lee
authors.forename: Yeon Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: In Young
authors.initials: IY
authors.lastname: Chung
authors.forename: Seong Wook
authors.initials: SW
authors.lastname: Seo
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
authors.forename: Ji Myung
authors.initials: JM
authors.lastname: Yoo
authors.forename: Jun Kyoung
authors.initials: JK
authors.lastname: Song
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015536:Down-Regulation; D015870:Gene Expression; D046228:Microarray Analysis; D010100:Oxygen; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism*; D012164:Retinal Diseases / Q000139:chemically induced* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D010100:Oxygen