title: Protein stability in the presence of cosolutes.
abstract: Protein scientists have long used cosolutes to study protein stability. While denaturants, such as urea, have been employed for a long time, the attention became focused more recently on protein stabilizers, including osmolytes. Here, we provide practical experimental instructions for the use of both stabilizing and denaturing osmolytes with proteins, as well as data evaluation strategies. We focus on protein stability in the presence of cosolutes and their mixtures at constant and variable temperature.
authors.affiliation: Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Luis Marcelo F
authors.initials: LM
authors.lastname: Holthauzen
authors.forename: Matthew
authors.initials: M
authors.lastname: Auton
authors.forename: Mikhail
authors.initials: M
authors.lastname: Sinev
authors.forename: Jörg
authors.initials: J
authors.lastname: Rösgen
mesh_terms: D000818:Animals; D006801:Humans; D009994:Osmolar Concentration; D017510:Protein Folding; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011506:Proteins