title: Small-angle X-ray scattering studies of peptide-lipid interactions using the mouse paneth cell α-defensin cryptdin-4.
abstract: In the presence of specialized proteins or peptides, a biological membrane can spontaneously restructure itself to allow communication between the intracellular and the extracellular sides. Examples of these proteins include cell-penetrating peptides and antimicrobial peptides (AMPs), which interact with cell membranes in complex ways. We briefly review cell-penetrating peptides and AMPs, and describe in detail how recombinant AMPs are made and their activity evaluated, using α-defensins as a specific example. We also review X-ray scattering methods used in studying peptide-membrane interactions, focusing on the procedures for small-angle X-ray scattering experiments on peptide-membrane interactions at realistic solution conditions, using both laboratory and synchrotron sources.
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, California, USA.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Mishra
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Tai
authors.forename: Nathan W
authors.initials: NW
authors.lastname: Schmidt
authors.forename: André J
authors.initials: AJ
authors.lastname: Ouellette
authors.forename: Gerard C L
authors.initials: GC
authors.lastname: Wong
mesh_terms: D000818:Animals; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D019879:Paneth Cells; D010455:Peptides / Q000737:chemistry*; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction / Q000379:methods*; D023084:alpha-Defensins / Q000737:chemistry
chemical_list: D008055:Lipids; D010455:Peptides; D023084:alpha-Defensins; C401847:cryptdin 4, mouse