title: The isolation of osteoclasts from human giant cell tumors and long-term marrow cultures.
abstract: The osteoclast is a large multinucleate cell formed from the fusion of mono-nuclear precursor cells of hemopoietic origin Unique markers of the osteoclast have been difficult to identify. Widely used techniques, such as histochemical location of tartrate-resistant acid phosphatase, and response to calciotropic hormones do not distinguish effectively between osteoclasts and non-osteo-clastic cells The only unequivocal evidence of osteoclast activity is the ability of the cells to excavate authentic resorption lacunae in vivo and in vitro (1.
authors.affiliation: Department of Human Anatomy and Cell Biology, University of Liverpool, UK.
authors.forename: C A
authors.initials: CA
authors.lastname: Walsh
authors.forename: J A
authors.initials: JA
authors.lastname: Carron
authors.forename: J A
authors.initials: JA
authors.lastname: Gallagher