title: HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus.
abstract: Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact that the hid-1 gene encodes a novel protein (HID-1) which is highly conserved from Caenorhabditis elegans to mammals, the domain structure, subcellular localization, and exact function of HID-1 remain unknown. Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain. In this study, we revealed that mammalian HID-1 localized to the medial- and trans- Golgi apparatus as well as the cytosol, and the localization was sensitive to brefeldin A treatment. Next, we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol. Finally, we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus. We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhan
authors.forename: Eli
authors.initials: E
authors.lastname: Song
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Yaming
authors.initials: Y
authors.lastname: Jiu
authors.forename: Wen
authors.initials: W
authors.lastname: Du
authors.forename: Jingze
authors.initials: J
authors.lastname: Lu
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
authors.forename: Pingyong
authors.initials: P
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D020126:Brefeldin A / Q000494:pharmacology; D045744:Cell Line, Tumor; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D021601:trans-Golgi Network / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; D020126:Brefeldin A