title: [Cryopreservation of Trichomonas vaginalis and its influence factors].
abstract: Different concentrations of dimethylsulfoxide (DMSO) and glycerol, various densities of Trichomonas vaginalis trophozoites and duration of storage were used to study the effects on the cryopreservation of T. vaginalis at -78 degrees C. The optimal densities of trichomonads for cryopreservation were (1-2) x10(6)/ml. Glycerol and DMSO showed a high protective effect, with a maximum effect at a concentration of 10%, and the survival rates were 38.0% and 31.7%, respectively. There was no significant difference between them (P > 0.05). The results showed that the above optimized factors can provide a good protection in short-term (1-16 weeks) cryopreservation, and the trichomonads can be incubated to a logarithmic growth phase at 37 degrees C.
authors.affiliation: Department of Parasitology, Basic Medical College of Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Yuan
authors.forename: Chang-Gui
authors.initials: CG
authors.lastname: Xue
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D003470:Culture Media; D004121:Dimethyl Sulfoxide; D014246:Trichomonas vaginalis / Q000254:growth & development*
chemical_list: D003451:Cryoprotective Agents; D003470:Culture Media; D004121:Dimethyl Sulfoxide