title: Replication and recombination of herpes simplex virus DNA.
abstract: Replication of herpes simplex virus takes place in the cell nucleus and is carried out by a replisome composed of six viral proteins: the UL30-UL42 DNA polymerase, the UL5-UL8-UL52 helicase-primase, and the UL29 single-stranded DNA-binding protein ICP8. The replisome is loaded on origins of replication by the UL9 initiator origin-binding protein. Virus replication is intimately coupled to recombination and repair, often performed by cellular proteins. Here, we review new significant developments: the three-dimensional structures for the DNA polymerase, the polymerase accessory factor, and the single-stranded DNA-binding protein; the reconstitution of a functional replisome in vitro; the elucidation of the mechanism for activation of origins of DNA replication; the identification of cellular proteins actively involved in or responding to viral DNA replication; and the elucidation of requirements for formation of replication foci in the nucleus and effects on protein localization.
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Isabella
authors.initials: I
authors.lastname: Muylaert
authors.forename: Ka-Wei
authors.initials: KW
authors.lastname: Tang
authors.forename: Per
authors.initials: P
authors.lastname: Elias
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000096:biosynthesis*; D006801:Humans; D011995:Recombination, Genetic / Q000502:physiology*; D018139:Simplexvirus / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins