title: Structural investigation of porcine stomach mucin by X-ray fiber diffraction and homology modeling.
abstract: The basic understanding of the three dimensional structure of mucin is essential to understand its physiological function. Technology has been developed to achieve orientated porcine stomach mucin molecules. X-ray fiber diffraction of partially orientated porcine stomach mucin molecules show d-spacing signals at 2.99, 4.06, 4.22, 4.7, 5.37 and 6.5 Å. The high intense d-spacing signal at 4.22 Å is attributed to the antiparallel β-sheet structure identified in the fraction of the homology modeled mucin molecule (amino acid residues 800-980) using Nidogen-Laminin complex structure as a template. The X-ray fiber diffraction signal at 6.5 Å reveals partial organization of oligosaccharides in porcine stomach mucin. This partial structure of mucin will be helpful in establishing a three dimensional structure for the whole mucin molecule.
authors.affiliation: Department of Physics, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu 627012, India. veluraja@msuniv.ac.in
authors.forename: K
authors.initials: K
authors.lastname: Veluraja
authors.forename: K N
authors.initials: KN
authors.lastname: Vennila
authors.forename: K
authors.initials: K
authors.lastname: Umamakeshvari
authors.forename: A
authors.initials: A
authors.lastname: Jasmine
authors.forename: D
authors.initials: D
authors.lastname: Velmurugan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005752:Gastric Mucins / Q000737:chemistry*; D008969:Molecular Sequence Data; D055263:Mucin-3 / Q000737:chemistry*; D017433:Protein Structure, Secondary; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D005752:Gastric Mucins; D055263:Mucin-3