title: [Individual response to ionising radiation: What predictive assay(s) to choose?].
abstract: Individual response to ionizing radiation is an important information required to apply an efficient radiotherapy treatment against tumour and to avoid any adverse effects in normal tissues. In 1981, Fertil and Malaise have demonstrated that the post-irradiation local tumor control determined in vivo is correlated with clonogenic cell survival assessed in vitro. Furthermore, these authors have reminded the relevance of the concept of intrinsic radiosensitivity that is specific to each individual organ (Fertil and Malaise, 1981) [1]. To date, since clonogenicity assays are too time-consuming and do not provide any other molecular information, a plethora of research groups have attempted to determine the molecular bases of intrinsic radiosensitivity in order to propose reliable and faster predictive assays. To this aim, several approaches have been developed. Notably, the recent revolution in genomic and proteomic technologies is providing a considerable number of data but their link with radiosensitivity still remains to be elucidated. On another hand, the systematic screening of some candidate genes potentially involved in the radiation response is highlighting the complexity of the molecular and cellular mechanisms of DNA damage sensoring and signalling and shows that an abnormal radiation response is not necessarily due to the impairment of one single protein. Finally, more modest approaches consisting in focusing some specific functions of DNA repair seem to provide more reliable clues to predict over-acute reactions caused by radiotherapy. In this review, we endeavoured to analyse the contributions of these major approaches to predict human radiosensitivity.
authors.affiliation: Inserm, U, groupe de radiobiologie, institut des neurosciences, chemin Fortuné-Ferrini, Grenoble, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Granzotto
authors.forename: Aurélie
authors.initials: A
authors.lastname: Joubert
authors.forename: Muriel
authors.initials: M
authors.lastname: Viau
authors.forename: Clément
authors.initials: C
authors.lastname: Devic
authors.forename: Mira
authors.initials: M
authors.lastname: Maalouf
authors.forename: Charles
authors.initials: C
authors.lastname: Thomas
authors.forename: Guillaume
authors.initials: G
authors.lastname: Vogin
authors.forename: Karim
authors.initials: K
authors.lastname: Malek
authors.forename: Catherine
authors.initials: C
authors.lastname: Colin
authors.forename: Jacques
authors.initials: J
authors.lastname: Balosso
authors.forename: Nicolas
authors.initials: N
authors.lastname: Foray
mesh_terms: D000818:Animals; D016923:Cell Death / Q000528:radiation effects; D015687:Cell Hypoxia; D002877:Chromosomes, Human / Q000528:radiation effects; D002999:Clone Cells / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000235:genetics / Q000532:radiotherapy; D011832:Radiation Injuries / Q000235:genetics / Q000517:prevention & control; D011836:Radiation Tolerance / Q000235:genetics* / Q000502:physiology; D011839:Radiation, Ionizing; D011874:Radiometry; D011878:Radiotherapy / Q000009:adverse effects; D016896:Treatment Outcome
chemical_list: D004247:DNA