title: Differential effects of the brefeldin A analogue (6R)-hydroxy-BFA in tobacco and Arabidopsis.
abstract: The effects of two brefeldin A (BFA) analogues (BFA lactam; 6(R)-hydroxy-BFA) on plant cells were tested. Although these two compounds elicited BFA-like effects in mammalian cells, the lactam analogue failed to elicit a response in plant cells. By contrast, while the 6(R)-hydroxy-BFA analogue gave rise to a classic BFA response in tobacco mesophyll protoplasts and true leaves of Arabidopsis (redistribution of Golgi enzymes into the ER), it failed to cause the formation of BFA-compartments in Arabidopsis root cells and cotyledonary leaves. Even when the GNL1-LM mutant of Arabidopsis, which has a cis-Golgi located BFA-sensitive ARF-GEF, was used, the 6(R)-hydroxy analogue failed to elicit a response at conventional BFA concentrations. Only at concentrations of over 200 μM did 6(R)-hydroxy-BFA elicit a BFA-like effect. These differences are interpreted in terms of the different properties of the respective TGN- (Arabidopsis roots) and cis-Golgi- (tobacco mesophyll) localized BFA-sensitive ARF-GEFs.
authors.affiliation: Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Langhans
authors.forename: Sebastian
authors.initials: S
authors.lastname: Förster
authors.forename: Günter
authors.initials: G
authors.lastname: Helmchen
authors.forename: David G
authors.initials: DG
authors.lastname: Robinson
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D020126:Brefeldin A / Q000031:analogs & derivatives* / Q000494:pharmacology*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism; D058503:Mesophyll Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D021601:trans-Golgi Network / Q000187:drug effects / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D015415:Biomarkers; C522273:GNL1 protein, Arabidopsis; D020662:Guanine Nucleotide Exchange Factors; D020126:Brefeldin A