title: [The effect of C3a, C5a and their receptor antagonists on the expression of beta-catenin in renal tubular cells].
abstract: OBJECTIVE
  To investigate the effect of C3a and C5a and their receptor antagonists (C3aRA and C5aRA) on the expression of BETAcatenin in renal tubular epithelial cell line HK-2.
  
  
  METHODS
  Cells were divided in into C3a group and C5a group which was further divided into four subgroups: C3a group (control, 1 micromol/L TGF-beta1, 50 nmol/L C3a, 1 micromol/L C3aRA); C5a group (control, 1 micromol/L TGF-beta1, 50 nmol/L C5a, 2.5 micromol/L C5aRA). Real time PCR and Western blot were used to detect mRNA and protein expression of beta-catenin.
  
  
  RESULTS
  Real-time PCR and Western blot demonstrated that 1 micromol/L TGF-beta1 could increase the expression of beta-catenin; C3a and C5a presented the similar inducible effect as TGF-beta1, which could be blocked by C3aR antagonist and C5aR antagonist (C3aRA and C5aRA).
  
  
  CONCLUSIONS
  Anaphylatoxin C3a and C5a can induce mRNA and protein expression of beta-catenin in renal tubular epithelial cells, which could be blocked by C3aRA and C5aRA. C3a and C5a may participate in tubular epithelial-mesenchymal transition in vitro.
authors.affiliation: Division of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Gou
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
mesh_terms: D002460:Cell Line; D015926:Complement C3a / Q000502:physiology*; D015936:Complement C5a / Q000502:physiology*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000037:antagonists & inhibitors* / Q000502:physiology; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C488033:C5AR1 protein, human; C495270:CTNNB1 protein, human; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D051176:beta Catenin; C085567:complement C3a receptor; D015926:Complement C3a; D015936:Complement C5a