title: Seed-based oral vaccines as allergen-specific immunotherapies.
abstract: Plant-based vaccines have advantages over conventional vaccines in terms of scalability, lack of requirement for cold chain logistics, stability, safety, cost-effectiveness and needle-free administration. In particular, when antigen is expressed in seeds, high production is possible and immunogenicity is not lost even if stocked at ambient temperature for several years. Induction of immune tolerance (desensitization) to allergen is a principle strategy for controlling allergic diseases, and is generally carried out by subcutaneous injection. Seed-based oral administration offers a straightforward and inexpensive alternative approach to deliver vaccines effectively to the GALT without loss of activity. Consumption of transgenic seeds containing modified hypo-allergenic tolerogen or T-cell epitope peptides derived from allergens has no or very few severe side effects and can induce immune tolerance leading to reduction of allergen-specific IgE production, T-cell proliferation and release of histamine. Suppression of allergen-specific clinical symptoms results. Thus, seed-based allergy vaccines offer an innovative and convenient allergen-specific immunotherapeutic approach as an alternative to conventional allergen-specific immunotherapy.
authors.affiliation: Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. takaiwa@nias.affrc.go.jp
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology; D000956:Antigens, Viral / Q000302:isolation & purification; D032485:Cryptomeria / Q000276:immunology; D003888:Desensitization, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D006801:Humans; D012275:Oryza; D010940:Plant Proteins / Q000276:immunology; D030821:Plants, Genetically Modified; D039981:Pyroglyphidae / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D055314:Seed Storage Proteins / Q000235:genetics; D012639:Seeds / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014612:Vaccines / Q000276:immunology*
chemical_list: D052179:Antigens, Plant; D000956:Antigens, Viral; C065290:Cry j I protein, Cryptomeria japonica; D018984:Epitopes, T-Lymphocyte; D010940:Plant Proteins; D011994:Recombinant Proteins; D055314:Seed Storage Proteins; D014612:Vaccines