title: Glutathione-S-transferase subtypes α and π as a tool to predict and monitor graft failure or regeneration in a pilot study of living donor liver transplantation.
abstract: OBJECTIVE
  Glutathione-S-transferase (GST) subtype α and π are differentially expressed in adult liver tissue. Objective of the study was if GST α and π may serve as predictive markers for liver surgery, especially transplantations.
  
  
  METHODS
  13 patients receiving living donor liver transplantation (LDLT) and their corresponding donors were analyzed for standard serum parameters (ALT, AST, γGT, bilirubin) as well as GST-α and -π before LDLT and daily for 10 days after LDLT. Patients (R) and donors (D) were grouped according to graft loss (R1/D1) or positive outcome (R2/D2) and above named serum parameters were compared between the groups.
  
  
  RESULTS
  R1 showed significantly increased GST-α and significantly lower GST-π levels than R2 patients or the donors. There was a positive correlation between GST-α and ALT, AST as well as bilirubin and a negative correlation to γGT. However, γGT correlated positively with GST-π. Graft failure was associated with combined low GST-π levels in donors and their recipients before living donor liver transplantation.
  
  
  CONCLUSIONS
  Our data suggest that high GST-α serum levels reflect ongoing liver damage while GST-π indicates the capacity and process of liver regeneration. Additionally, GST-π may be useful as marker for optimizing donor and recipient pairs in living donor liver transplantation.
authors.affiliation: Clinic of Gastroenterology and Hepatology, University Hospital Essen, Hufelandstr. 55, 45122 Essen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Jochum
authors.forename: M
authors.initials: M
authors.lastname: Beste
authors.forename: J-P
authors.initials: JP
authors.lastname: Sowa
authors.forename: M S
authors.initials: MS
authors.lastname: Farahani
authors.forename: V
authors.initials: V
authors.lastname: Penndorf
authors.forename: S
authors.initials: S
authors.lastname: Nadalin
authors.forename: F
authors.initials: F
authors.lastname: Saner
authors.forename: A
authors.initials: A
authors.lastname: Canbay
authors.forename: Guido
authors.initials: G
authors.lastname: Gerken
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D051549:Glutathione S-Transferase pi / Q000097:blood*; D005982:Glutathione Transferase / Q000097:blood*; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008099:Liver / Q000201:enzymology*; D008111:Liver Function Tests; D008115:Liver Regeneration / Q000502:physiology; D016031:Liver Transplantation / Q000502:physiology; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011237:Predictive Value of Tests; D011379:Prognosis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha