title: TFCP2c/LSF/LBP-1c is required for Snail1-induced fibronectin gene expression.
abstract: Fibronectins are cell-secreted glycoproteins that modulate cell attachment, spreading, migration, morphology, differentiation and oncogenic transformation. Fibronectin expression is activated during EMT (epithelial-mesenchymal transition) and is a hallmark of mesenchymal cells. It is shown in the present study that a transcription factor previously unrelated with EMT, TFCP2c/LSF/LBP-1c, was translocated to the nucleus and bound to the fibronectin promoter upon EMT induction by Snail1. Consequently, the interference of TFCP2c/LSF/LBP-1c's activity prevented fibronectin expression. Moreover, TFCP2c/LSF/LBP-1c was detected in nuclei of embryonic dermal mesenchymal cells adjacent to the hair bud, a cell population that expresses endogenous nuclear Snail1 and fibronectin. Therefore we indicate a new molecular role for TFCP2c/LSF/LBP-1c in fibronectin expression.
authors.affiliation: Programa de Recerca en Càncer, IMIM-Hospital del Mar, E-08003, Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Porta-de-la-Riva
authors.forename: Jelena
authors.initials: J
authors.lastname: Stanisavljevic
authors.forename: Josue
authors.initials: J
authors.lastname: Curto
authors.forename: Clara
authors.initials: C
authors.lastname: Francí
authors.forename: Víctor Manuel
authors.initials: VM
authors.lastname: Díaz
authors.forename: Antonio
authors.initials: A
authors.lastname: García de Herreros
authors.forename: Josep
authors.initials: J
authors.lastname: Baulida
mesh_terms: D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D021381:Protein Transport / Q000502:physiology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D005353:Fibronectins; C000604407:SNAI1 protein, human; C000604429:Snai1 protein, mouse; D000071250:Snail Family Transcription Factors; C501905:Tfcp2 protein, mouse; D014157:Transcription Factors