title: [Correlation between chlamydia pneumoniae IgG positive in lung cancer patients and cytokines related to radiation-induced pulmonary lesion].
abstract: OBJECTIVE
  There exists intimate relationship between infection with chlamydia pneumoniae (Cpn) and lung cancer incidence. But few studies have been reported about radiation-induced pulmonary lesion in lung cancer patients infected with Cpn. The aim of this study is to explore the correlation between cytokines related to radiation-induced pulmonary lesion and Cpn IgG positive in lung cancer patients.
  
  
  METHODS
  A total of 69 patients with lung cancer received chest radiotherapy. Blood samples were collected and frozen before radiotherapy (pre-RT), middle radiotherapy (mid-RT) and after radiotherapy (post-RT). Cpn IgG and levels of IL-1β, SP-A, TGF-β, and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  In the total of 69 patients, 21 patients were Cpn IgG positive, 48 patients negative. The positive rate was 30.43%. In mid-RT concentration of IL-1β in Cpn IgG positive and negative group were (35.82±10.09) ng/L and (30.01±6.46) ng/L, with statistically significant difference (P < 0.05). Pre-RT and post-RT concentrations of IL-1β in Cpn IgG positive and negative group had no statistically significant difference. Mid-RT concentrations of SP-A in Cpn IgG positive group and negative group were (641.78±106.81) ng/L and (100.86±61.4) ng/L respectively, with statistically significant difference (P < 0.05). Post-RT concentration of SP-A in Cpn IgG positive and negative group were (657.47±115.19) ng/L and (93.23±47.15) ng/L respectively, with statistically significant difference (P < 0.05). Concentrations of TNF-α in Cpn IgG positive and negative group had no statistically significant difference. Concentrations of TGF-β in Cpn IgG positive group were (710.67±358.16) pg/mL in pre-RT, (1,002.06±542.16) pg/mL in mid-RT, (2,125.16±1,522.29) pg/mL in post-RT; those in negative group were (867.77±412.48) pg/mL, (914.05±425.70) pg/mL, (1,073.36±896.01) pg/mL. Concentration of TGF-β in post-RT between Cpn IgG positive and negative group had statistically significant difference (P < 0.05).
  
  
  CONCLUSIONS
  Cpn IgG positive in lung cancer patients influenced levels of IL-1β, SP-A, TGF-β during chest radiotherapy. This might aggravate radiation-induced pulmonary lesion.
authors.affiliation: Department of Radiation Oncology, Jinshan Hospital, Fudan University, Shanghai 200540, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
authors.forename: Tiankui
authors.initials: T
authors.lastname: Qiao
authors.forename: Daoan
authors.initials: D
authors.lastname: Zhou
authors.forename: Sujuan
authors.initials: S
authors.lastname: Yuan
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology; D023521:Chlamydophila Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology / Q000532:radiotherapy*; D016993:Chlamydophila pneumoniae / Q000276:immunology / Q000302:isolation & purification; D016207:Cytokines / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000382:microbiology / Q000473:pathology / Q000532:radiotherapy*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D016207:Cytokines; D007074:Immunoglobulin G