title: Inhibin B and anti-Müllerian hormone as markers of gonadal function after hematopoietic cell transplantation during childhood.
abstract: BACKGROUND
  It is difficult to predict the reproductive capacity of children given hematopoietic cell transplantation (HCT) before pubertal age because the plasma concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are not informative and no spermogram can be done.
  
  
  METHODS
  We classified the gonadal function of 38 boys and 34 girls given HCT during childhood who had reached pubertal age according to their pubertal development and FSH and LH and compared this to their plasma inhibin B and anti-Müllerian hormone (AMH).
  
  
  RESULTS
  Ten (26%) boys had normal testicular function, 16 (42%) had isolated tubular failure and 12 (32%) also had Leydig cell failure. All 16 boys given melphalan had tubular failure. AMH were normal in 25 patients and decreased in 6, all of whom had increased FSH and low inhibin B.Seven (21%) girls had normal ovarian function, 11 (32%) had partial and 16 (47%) complete ovarian failure. 7/8 girls given busulfan had increased FSH and LH and 7/8 had low inhibin B. AMH indicated that ovarian function was impaired in all girls.FSH and inhibin B were negatively correlated in boys (P < 0.0001) and girls (P = 0.0006). Neither the age at HCT nor the interval between HCT and evaluation influenced gonadal function.
  
  
  CONCLUSIONS
  The concordance between FSH and inhibin B suggests that inhibin B may help in counselling at pubertal age. In boys, AMH were difficult to use as they normally decrease when testosterone increases at puberty. In girls, low AMH suggest that there is major loss of primordial follicles.
authors.affiliation: Université Paris Descartes and AP-HP, Hôpital Bicêtre, Unité d'Endocrinologie Pédiatrique, Le Kremlin Bicêtre, France. raja.brauner@wanadoo.fr
authors.forename: Sylvie
authors.initials: S
authors.lastname: Laporte
authors.forename: Ana-Claudia
authors.initials: AC
authors.lastname: Couto-Silva
authors.forename: Séverine
authors.initials: S
authors.lastname: Trabado
authors.forename: Pierre
authors.initials: P
authors.lastname: Lemaire
authors.forename: Sylvie
authors.initials: S
authors.lastname: Brailly-Tabard
authors.forename: Hélène
authors.initials: H
authors.lastname: Espérou
authors.forename: Jean
authors.initials: J
authors.lastname: Michon
authors.forename: André
authors.initials: A
authors.lastname: Baruchel
authors.forename: Alain
authors.initials: A
authors.lastname: Fischer
authors.forename: Christine
authors.initials: C
authors.lastname: Trivin
authors.forename: Raja
authors.initials: R
authors.lastname: Brauner
mesh_terms: D000293:Adolescent; D054304:Anti-Mullerian Hormone / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006058:Gonadal Disorders / Q000097:blood / Q000175:diagnosis* / Q000209:etiology* / Q000503:physiopathology; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007265:Inhibins / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010053:Ovary / Q000503:physiopathology; D012189:Retrospective Studies; D013737:Testis / Q000503:physiopathology; D019172:Transplantation Conditioning / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; C422364:inhibin B; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone