title: Hot transcriptomics.
abstract: DNA microarray technology allows for a quick and easy comparison of complete transcriptomes, resulting in improved molecular insight in fluctuations of gene expression. After emergence of the microarray technology about a decade ago, the technique has now matured and has become routine in many molecular biology laboratories. Numerous studies have been performed that have provided global transcription patterns of many organisms under a wide range of conditions. Initially, implementation of this high-throughput technology has lead to high expectations for ground breaking discoveries. Here an evaluation is performed of the insight that transcriptome analysis has brought about in the field of hyperthermophilic archaea. The examples that will be discussed have been selected on the basis of their impact, in terms of either biological insight or technological progress.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Jasper
authors.initials: J
authors.lastname: Walther
authors.forename: Pawel
authors.initials: P
authors.lastname: Sierocinski
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis