title: The histone H2A variant macroH2A1 does not localize to the centrosome.
abstract: MacroH2A1 is a histone H2A variant which contains a large non-histone C-terminal region of largely unknown function. Within this region is a macro domain which can bind ADP-ribose and related molecules. Most studies of macroH2A1 focus on the involvement of this variant in transcriptional repression. Studies in mouse embryos and in embryonic stem cells suggested that during early development macroH2A can be found at the centrosome. Centrosomal localization of macroH2A was later reported in somatic cells. Here we provide data showing that macroH2A1 does not localize to the centrosome and that the centrosomal signal observed with antibodies directed against the macroH2A1 non-histone region may be the result of antibody cross-reactivity.
authors.affiliation: Department of Cell and Developmental Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Friedman
authors.forename: Michal
authors.initials: M
authors.lastname: Barzily-Rokni
authors.forename: Sara
authors.initials: S
authors.lastname: Isaac
authors.forename: Amir
authors.initials: A
authors.lastname: Eden
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D055785:Gene Knockdown Techniques; D014644:Genetic Variation / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006657:Histones / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D002843:Chromatin; D006657:Histones; D050505:Mutant Proteins; D011993:Recombinant Fusion Proteins; C120756:macroH2A histone; D049452:Green Fluorescent Proteins