title: Expression and proliferative effect of hemokinin-1 in human B-cells.
abstract: Tachykinins are a family of structurally related peptides, including substance P (SP), hemokinin-1 (HK-1), neurokinin A (NKA), and neurokinin B. SP and NKA have been shown to modulate hematopoiesis and rat/mouse HK-1 has been found to be involved in the survival and differentiation of mouse B-cells. This study was designed to assess the expression of tachykinins with a focus on human HK-1 (hHK-1) in human B lymphocytes and the role of these peptides in cell differentiation, apoptosis and proliferation. Expression of tachykinin and tachykinin receptor mRNA was determined quantitatively in human B lymphoproliferative malignancies and compared to normal B-cells. Expression of hHK-1 and NK(1) receptor, but not SP, was detected in human B-lymphocytes, and was up-regulated in B-lymphocytes from chronic lymphocytic leukemia and non-Hodgkin's lymphoma, while it was down-regulated in acute lymphoblastic leukemia. Moreover, hHK-1, in contrast to SP, was able to induce proliferation of human pre-B lymphocytes through a NK(1) receptor-independent mechanism. These data suggest a role for hHK-1 in normal and pathological B lymphopoiesis, and open the door to a better understanding of the physiopathological mechanisms leading to lymphoproliferative malignancies.
authors.affiliation: Laboratory of Pulmonary Pharmacology, UPRES EA220, Foch Hospital, University Versailles - Saint Quentin en Yvelines, Suresnes, France. s.grassindelyle@gmail.com
authors.forename: Stanislas
authors.initials: S
authors.lastname: Grassin-Delyle
authors.forename: Amparo
authors.initials: A
authors.lastname: Buenestado
authors.forename: Laurent
authors.initials: L
authors.lastname: Vallat
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Naline
authors.forename: Sirima
authors.initials: S
authors.lastname: Marx
authors.forename: Julie
authors.initials: J
authors.lastname: Decocq
authors.forename: Patrice
authors.initials: P
authors.lastname: Debré
authors.forename: Olivier A
authors.initials: OA
authors.lastname: Bernard
authors.forename: Charles
authors.initials: C
authors.lastname: Advenier
authors.forename: Philippe
authors.initials: P
authors.lastname: Devillier
authors.forename: Hélène
authors.initials: H
authors.lastname: Merle-Béral
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018040:Receptors, Neurokinin-1 / Q000235:genetics; D018039:Receptors, Tachykinin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015320:Tachykinins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D018040:Receptors, Neurokinin-1; D018039:Receptors, Tachykinin; C486037:TAC4 protein, human; D015320:Tachykinins; C507968:hemokinin-1