title: Spectrophotometric study on the binding of two water soluble Schiff base complexes of Mn(III) with ct-DNA.
abstract: In this work, binding of two water soluble Schiff base complexes: Bis sodium (5-sulfosalicylaldehyde) o-phenylendiiminato) Manganese (III) acetate (Salophen complex) and Bis sodium (5-sulfosalicylaldehyde) 1, 2 ethylendiiminato) Manganese (III) acetate (Salen complex) with calf thymus (ct) DNA were investigated by using different spectroscopic and electrometric techniques including UV-vis, Circular dichroism (CD) and fluorescence spectroscopy, viscommetry and cyclic voltammetry (CV). Both complexes have shown a hyperchromic and a small bathochromic shift in the visible region spectra. A competitive binding study showed that the enhanced emission intensity of ethidium bromide (EB) in the presence of DNA was quenched by the addition of the two Schiff base complexes indicating that they displace EB from its binding site in DNA. Moreover structural changes in the CD spectra and an increase in the CV spectra with addition of DNA were observed. The results show that both complexes bind to DNA. The binding constants have been calculated using fluorescence data for two complexes also K(b) was calculated with fluorescence Scatchard plot for Salophen. Ultimately, the experimental results show that the dominant interactions are electrostatic while binding mode is surface binding then followed by hydrophobic interactions in grooves in high concentration of complexes.
authors.affiliation: Laboratory of Biophysical Chemistry, Department of Chemistry, Isfahan University, Isfahan 81746-73441, Iran. nejatmari@yahoo.com
authors.forename: Maryam Nejat
authors.initials: MN
authors.lastname: Dehkordi
authors.forename: Abdol-Khlegh
authors.initials: AK
authors.lastname: Bordbar
authors.forename: Masood Ayatolahi
authors.initials: MA
authors.lastname: Mehrgardi
authors.forename: Valiolah
authors.initials: V
authors.lastname: Mirkhani
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D008345:Manganese / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D011489:Protein Denaturation; D012545:Schiff Bases / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014783:Viscosity; D014867:Water / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D012545:Schiff Bases; D014867:Water; D008345:Manganese; D012965:Sodium Chloride; D004247:DNA; C427203:calf thymus DNA