title: FGF2-labeled semiconductor nanocrystals as luminescent biolabels for imaging neuroblastoma cells.
abstract: In this paper, we report the synthesis of FGF-2 peptide labeled luminescent nanocrystals as optical probes for targeted imaging of live neuroblastoma cells. Confocal laser scanning microscopy technique was used to confirm the receptor-mediated uptake of the bioconjugates into the live neuroblastoma cells and study the dynamic profile of the bioconjugates within a single cell. It was found that the bioconjugates were self-assembled near the nuclear periphery after they entered the cellular cytoplasm. In addition to confocal imaging, the cytotoxicity of bioconjugates was investigated using colorimetric cell viability (MTS) assay. The MTS study reveals that the bioconjugate formulation has very low toxicity. These results demonstrate that the luminescent nanocrystal bioconjugates can serve as biocompatible targeted probes for multiplex cell labeling and molecule tracking.
authors.affiliation: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province, Institute of Optoelectronics, Shenzhen University, Shenzhen 518060, P. R. China.
authors.forename: Gaixia
authors.initials: G
authors.lastname: Xu
authors.forename: Ken-Tye
authors.initials: KT
authors.lastname: Yong
authors.forename: Indrajit
authors.initials: I
authors.lastname: Roy
authors.forename: Atcha
authors.initials: A
authors.lastname: Kopwitthaya
mesh_terms: D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D006801:Humans; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry*; D009447:Neuroblastoma / Q000175:diagnosis*; D045663:Quantum Dots
chemical_list: D049408:Luminescent Agents; D016222:Fibroblast Growth Factor 2