title: T-body formation precedes virus-like particle maturation in S. cerevisiae.
abstract: T-bodies are localized S. cerevisiae RNPs containing Ty1 retroviral components and speculated to play a role in the assembly of virus-like particles (VLPs). Mapping requirements for T-body formation, we demonstrate that ectopic expression of immature TyA1/Gag (Gag-p49), a structural component of the Ty1 capsid, is sufficient for T-body formation both under normal conditions as well as in a strain background devoid of endogenous Gag. Moreover, T-bodies are readily formed when Ty1 transposition is blocked. Thus, T-bodies represent an early stage in the Ty1 life cycle, preceding VLP maturation.
authors.affiliation: Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, Aarhus C., Denmark. fm@mb.au.dk or thj@mb.au.dk
authors.forename: Francisco
authors.initials: F
authors.lastname: Malagon
authors.forename: Torben Heick
authors.initials: TH
authors.lastname: Jensen
mesh_terms: D015966:Gene Expression Regulation, Fungal; D015683:Gene Products, gag / Q000235:genetics* / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D012261:Ribonucleoproteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
chemical_list: D015683:Gene Products, gag; D018626:Retroelements; D012261:Ribonucleoproteins