title: Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.
abstract: The Bacillus subtilis structural gene pgsE was investigated as a tool for extrachromosomal DNA maintenance (EDM). It ameliorated the stability of high-copy-number vectors, regardless of whether they were derived from rolling-cycle or theta-mode replicons, without any selective pressure. This unique EDM phenomenon may occur via a trans-acting mechanism.
authors.affiliation: Course of Applied Bioresource Science, United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamashiro
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Minouchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Ashiuchi
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005970:Glutamate Synthase / Q000235:genetics* / Q000378:metabolism; D011099:Polyglutamic Acid / Q000096:biosynthesis / Q000235:genetics; D012093:Replicon / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D011099:Polyglutamic Acid; D005970:Glutamate Synthase