title: Impact of microencapsulated peptidase (Aspergillus oryzae) on cheddar cheese proteolysis and its biologically active peptide profile.
abstract: We investigated the delivery of calcium-alginate encapsulated peptidase (Flavourzyme(®), Aspergillus oryzae) on proteolysis of Cheddar cheese. Physical and chemical characteristics such as moisture, pH and fat content were measured, and no differences were found between control and experimental cheese at day 0. SDS-PAGE analysis clearly showed that proteolysis of α and k casein was significantly accelerated after three months of maturity in the experimental cheese. A large number of low molecular weight peptides were found in the water soluble fraction of the experimental cheeses and some of these peptides were new. N-terminal amino acid sequence analysis identified these as P(1), Leu-Thu-Glu; P(3), Asp-Val-Pro-Ser-Glu) and relatively abundant stable peptides P(2), P(4), Arg-Pro-Lys-His-Pro-Ile; P(5), Arg-Pro-Lys-His-Pro-Ile-Lys and P(6). These peptides were mainly originated from αs1-CN and β-CN. Three of the identified peptides (P(1), P(2), P(3) and P(4)) are known to biologically active and P(1) and P(3) were only present in experimental cheese suggesting that experimental cheese has improved health benefits.
authors.affiliation: Department of Agriculture and Food Systems, Melbourne School of Land and Environment, The University of Melbourne, Private Box 260, Horsham, Vic 3401 Australia. samans@unimelb.edu.au
authors.forename: Saman
authors.initials: S
authors.lastname: Seneweera
authors.forename: Kaila
authors.initials: K
authors.lastname: Kailasapathy
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D002611:Cheese / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D059748:Proteolysis
chemical_list: D010455:Peptides; D010447:Peptide Hydrolases