title: Generation of pluripotent cancer-initiating cells from transformed bone marrow-derived cells.
abstract: Our previous studies indicated that pluripotent cancer-initiating cells existed in chemical carcinogen-transformed mouse bone marrow-derived cells (BMDCs). In this report, we named them as bone marrow-derived pluripotent cancer-initiating (bmPCI) cells. The bmPCI cells showed properties of embryonal carcinoma (EC) cells/embryonic stem (ES) cells in morphology and gene expression, differentiated into multiple somatic tumor cells, generated oocyte-like cells and later developed into blastocyst-like structures in vitro while causing teratocarcinomas in vivo. These findings demonstrate that pluripotent cancer-initiating cells can originate from BMDCs. It provides an accessible in vitro model for copying the "embryonal rest" phenomenon from adult cells.
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Liu
authors.forename: Songtao
authors.initials: S
authors.lastname: Xu
authors.forename: Zhan
authors.initials: Z
authors.lastname: Ma
authors.forename: Yu
authors.initials: Y
authors.lastname: Zeng
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D018236:Carcinoma, Embryonal / Q000378:metabolism*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D018243:Teratocarcinoma / Q000378:metabolism