title: Induction of β-1,3-glucanase in seeds of maize defective-kernel mutant (827Kpro1).
abstract: β-1,3-glucanases are found in organisms as diverse as plants, animals, bacteria and fungi. In plants, such enzymes are not only associated with defense mechanisms against pathogens, but also play critical roles in physiological and developmental processes. Here we identified a new β-1,3-glucanase in maize seeds, and named it ZmGlucA. Sequence analysis revealed that ZmGlucA belongs to the class A of β-1,3-glucanase, a class related to defense and physiological processes in plants. mRNA and protein assays showed that zmGlucA is expressed exclusively in seeds, and it is differentially regulated during seed development. Additionally, zmGlucA expression is strongly induced in seeds of the mutant dek 827Kpro1, which is defective for embryo and endosperm development. Our data support the idea that ZmGlucA protein is relevant to seed development.
authors.affiliation: Universidade Estadual do Norte Fluminense Darcy Ribeiro - Centro de Biociências e Biotecnologia - Laboratório de Biotecnologia - Av. Alberto Lamego, 2000 - Parque Califórnia - Campos dos Goytacazes - CEP 28013-602 - R.J. - Brazil.
authors.forename: Alan Trindade
authors.initials: AT
authors.lastname: Branco
authors.forename: Beatriz
authors.initials: B
authors.lastname: Dos Santos Ferreira
authors.forename: Guinevere Fernandes
authors.initials: GF
authors.lastname: Lourenço
authors.forename: Valéria Cristina
authors.initials: VC
authors.lastname: Lopes Marques
authors.forename: Olga Lima
authors.initials: OL
authors.lastname: Tavares Machado
authors.forename: Messias Gonzaga
authors.initials: MG
authors.lastname: Pereira
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Aquino Almeida
authors.forename: Gonçalo Apolinário
authors.initials: GA
authors.lastname: de Souza Filho
mesh_terms: D015152:Blotting, Northern; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D012639:Seeds / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*
chemical_list: D006026:Glycoside Hydrolases