title: Isolation and transcriptome analysis of adult zebrafish cells enriched for skeletal muscle progenitors.
abstract: BACKGROUND
  Over the past 10 years, the use of zebrafish for scientific research in the area of muscle development has increased dramatically. Although several protocols exist for the isolation of adult myoblast progenitors from larger fish, no standardized protocol exists for the isolation of myogenic progenitors from adult zebrafish muscle.
  
  
  METHODS
  Using a variant of a mammalian myoblast isolation protocol, zebrafish muscle progenitors have been isolated from the total dorsal myotome. These zebrafish myoblast progenitors can be cultured for several passages and then differentiated into multinucleated, mature myotubes.
  
  
  RESULTS
  Transcriptome analysis of these cells during myogenic differentiation revealed a strong downregulation of pluripotency genes, while, conversely, showing an upregulation of myogenic signaling and structural genes.
  
  
  CONCLUSIONS
  Together these studies provide a simple, yet detailed method for the isolation and culture of myogenic progenitors from adult zebrafish, while further promoting their therapeutic potential for the study of muscle disease and drug screening.
authors.affiliation: Howard Hughes Medical Institute, Center for Life Science, Room CLS15027.1, Children's Hospital Boston, 3 Blackfan Circle, Boston, Massachusetts 02115, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Alexander
authors.forename: Genri
authors.initials: G
authors.lastname: Kawahara
authors.forename: Alvin T
authors.initials: AT
authors.lastname: Kho
authors.forename: Melanie H
authors.initials: MH
authors.lastname: Howell
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Pusack
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Myers
authors.forename: Federica
authors.initials: F
authors.lastname: Montanaro
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Guyon
authors.forename: Louis M
authors.initials: LM
authors.lastname: Kunkel
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods*; D024510:Muscle Development / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D015027:Zebrafish