title: [Protective effects of tianji capsule on vascular endothelial cells from oxidative injury induced by hydrogen peroxide in vitro].
abstract: OBJECTIVE
  To study the protective effects of Tianji capsule (TJ) on vascular endothelial cells from oxidative injury induced by hydrogen peroxide and its possible mechanism of anti-oxidation.
  
  
  METHODS
  The effect of TJ on the proliferation of normal human umbilical vascular endothelial cells (HUVECs) as well as its cytotoxicity was evaluated with methylthiazolyl tetrazolium (MTT) assay. After the establishment of oxidative injury model of HUVECs, control, oxidative injury model, TJ and CoQ10 treatment groups were set up. HUVECs were incubated with 37.5, 75, 150 and 300 microg/mL TJ or 100 microg/mL CoQ10 for 24 h, and 0.1 mmol/L H2O2 (final concentration) was added to HUVECs in each groups for 30 min. Then collected the cells for proliferation detection with MTT assay, and the levels of MDA and NO, the activities of SOD, GSH-Px and NOS, as well as the releasing rate of LDH in HUVECs were also determined.
  
  
  RESULTS
  No cytotoxicity was observed in HUVECs with less than 400 microg/ mL TJ incubated for 48 h, but increased proliferation rates were noticed. Pretreated with TJ (37.5, 75, 150 and 300 microg/mL), increased proliferation rate, the activities of SOD, GSH-Px, NOS were observed, but the decreased level of MDA and releasing rate of LDH were also found.
  
  
  CONCLUSIONS
  TJ could protect HUVECs against oxidative injury induced by H2O2.
authors.affiliation: Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Ma
authors.forename: Jin-yao
authors.initials: JY
authors.lastname: Chen
authors.forename: Li-shi
authors.initials: LS
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002214:Capsules; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000473:pathology*; D006801:Humans; D006861:Hydrogen Peroxide; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D002214:Capsules; D004365:Drugs, Chinese Herbal; D006861:Hydrogen Peroxide