title: Research of UL54-specific siRNA on herpes simplex virus type II replication.
abstract: To determine how UL54-specific siRNA affects virus replication and protection of host cells, we examined virus titer and the activity of the cells at 12 hours, 24 hours, 36 hours, 48 hours, 60 hours and 72 hours after process of RNAi, including: four UL54-specific siRNAs and the positive/negative control siRNAs synthesized in vitro by chemical processes. The Vero cells were transfected with siRNAs using lipofectamine 2000 followed by infection by HSV-II. Our studies reveal that the groups with UL54-specific siRNA decreased significantly in virus titer at 12-24 hours, and only slightly decreased after that; groups with UL54-specific siRNA had higher OD values shown by MTT colorimetric assay than blank cells and survived better; R2 and R4 groups had lower virus titer and better survival than other groups. UL54-specific siRNA can inhibit HSV-II replication, while protecting host cells. There are effective and ineffective siRNA, which were synthesized in accordance with the same principles.
authors.affiliation: Department of Dermatology and Venereology, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Qing
authors.forename: Weng
authors.initials: W
authors.lastname: Weili
authors.forename: Zeng
authors.initials: Z
authors.lastname: Fanqin
authors.forename: Zheng
authors.initials: Z
authors.lastname: Rongchang
authors.forename: Luo
authors.initials: L
authors.lastname: Yijin
authors.forename: Du
authors.initials: D
authors.lastname: Jianqun
mesh_terms: D000818:Animals; D002470:Cell Survival; D002522:Chlorocebus aethiops; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000254:growth & development*; D017874:Immediate-Early Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014162:Transfection; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D017874:Immediate-Early Proteins; D034741:RNA, Small Interfering; C558833:UL54 protein, herpes simplex virus type II; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase