title: Simultaneous analysis of hydrochlorothiazide, triamterene and reserpine in rat plasma by high performance liquid chromatography and tandem solid-phase extraction.
abstract: A tandem solid-phase extraction method (SPE) of connecting two different cartridges (C(18) and MCX) in series was developed as the extraction procedure in this article, which provided better extraction yields (>86%) for all analytes and more appropriate sample purification from endogenous interference materials compared with a single cartridge. Analyte separation was achieved on a C(18) reversed-phase column at the wavelength of 265 nm by high-performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89% with RSD values that were always less than 3.8%. The method has been successfully applied to plasma samples from rats after oral administration of target compounds.
authors.affiliation: Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, Guangzhou, PR China.
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.forename: Junting
authors.initials: J
authors.lastname: He
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huo
authors.forename: Si
authors.initials: S
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics / Q000097:blood / Q000302:isolation & purification; D006852:Hydrochlorothiazide / Q000097:blood* / Q000302:isolation & purification*; D051381:Rats; D012110:Reserpine / Q000097:blood* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D014223:Triamterene / Q000097:blood* / Q000302:isolation & purification*
chemical_list: D004232:Diuretics; D006852:Hydrochlorothiazide; D012110:Reserpine; D014223:Triamterene