title: Molluscan catch muscle myorod and its N-terminal peptide bind to F-actin and myosin in a phosphorylation-dependent manner.
abstract: Myorod is expressed exclusively in molluscan catch muscle and localizes on the surface of thick filaments together with twitchin and myosin. This protein is an alternatively spliced product of the myosin heavy-chain gene containing the C-terminal rod part of myosin and a unique N-terminal domain. We have recently reported that this unique domain is a target for phosphorylation by gizzard smooth muscle myosin light chain kinase (MLCK) and molluscan twitchin, which contains a MLCK-like domain. To elucidate the role of myorod phosphorylation in catch muscle, a peptide corresponding to the specific N-terminal region of the protein was synthesized in phosphorylated and unphosphorylated form. We report, for the first time, that unphosphorylated full-length myorod and its unphosphorylated N-terminal synthetic peptide are able to interact with rabbit F-actin and thin filaments from molluscan catch muscle. The binding between thin filaments and the peptide was Ca²+-dependent. In addition, we found that phosphorylated N-terminal peptide of myorod has higher affinity for myosin compared to the unphosphorylated peptide. Together, these observations suggest the direct involvement of the N-terminal domain of myorod in the regulation of molluscan catch muscle.
authors.affiliation: Laboratory of Cell Biophysics, A.V. Zhirmunsky Institute of Marine Biology, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia. osmatusovsky@gmail.com
authors.forename: Oleg S
authors.initials: OS
authors.lastname: Matusovsky
authors.forename: Galina G
authors.initials: GG
authors.lastname: Matusovskaya
authors.forename: Vyacheslav A
authors.initials: VA
authors.lastname: Dyachuk
authors.forename: Nikolay S
authors.initials: NS
authors.lastname: Shelud'ko
mesh_terms: D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D049879:Mytilidae / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D011817:Rabbits
chemical_list: D000199:Actins; D009124:Muscle Proteins; D009218:Myosins