title: Viral RNAi suppressor reversibly binds siRNA to outcompete Dicer and RISC via multiple turnover.
abstract: RNA interference is a conserved gene regulatory mechanism employed by most eukaryotes as a key component of their innate immune response to viruses and retrotransposons. During viral infection, the RNase-III-type endonuclease Dicer cleaves viral double-stranded RNA into small interfering RNAs (siRNAs) 21-24 nucleotides in length and helps load them into the RNA-induced silencing complex (RISC) to guide the cleavage of complementary viral RNA. As a countermeasure, many viruses have evolved viral RNA silencing suppressors (RSS) that tightly, and presumably quantitatively, bind siRNAs to thwart RNA-interference-mediated degradation. Viral RSS proteins also act across kingdoms as potential immunosuppressors in gene therapeutic applications. Here we report fluorescence quenching and electrophoretic mobility shift assays that probe siRNA binding by the dimeric RSS p19 from Carnation Italian Ringspot Virus, as well as by human Dicer and RISC assembly complexes. We find that the siRNA:p19 interaction is readily reversible, characterized by rapid binding [(1.69 ± 0.07) × 10(8) M(-)(1) s(-1)] and marked dissociation (k(off)=0.062 ± 0.002 s(-1)). We also observe that p19 efficiently competes with recombinant Dicer and inhibits the formation of RISC-related assembly complexes found in human cell extract. Computational modeling based on these results provides evidence for the transient formation of a ternary complex between siRNA, human Dicer, and p19. An expanded model of RNA silencing indicates that multiple turnover by reversible binding of siRNAs potentiates the efficiency of the suppressor protein. Our predictive model is expected to be applicable to the dosing of p19 as a silencing suppressor in viral gene therapy.
authors.affiliation: Biophysics, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109-1055, USA.
authors.forename: Renata A
authors.initials: RA
authors.lastname: Rawlings
authors.forename: Vishalakshi
authors.initials: V
authors.lastname: Krishnan
authors.forename: Nils G
authors.initials: NG
authors.lastname: Walter
mesh_terms: D015153:Blotting, Western; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D008433:Mathematics; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D034743:RNA-Induced Silencing Complex / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D018626:Retroelements; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017864:Tombusvirus / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C493885:P19 protein, tomato bushy stunt virus; D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D012367:RNA, Viral; D034743:RNA-Induced Silencing Complex; D011994:Recombinant Proteins; D018626:Retroelements; D014764:Viral Proteins; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases