title: Full-length transcriptome analysis using a bias-free cDNA library prepared with the vector-capping method.
abstract: Full-length complementary DNAs (cDNAs) are an essential resource for functional genomics. Recently, we have developed a simple and efficient method for preparing a full-length cDNA library from a small amount of total RNA, named the "vector-capping" method. The biggest advantage of this method is that the intactness of the cDNA can be assured by the presence of dG at the 5' end of the full-length cDNA. Furthermore, the cDNA library represents the mRNA population in the cell owing to a bias-free procedure. In this chapter, we describe not only the protocol for preparing the library but also the points for analyzing the 5'-end sequence of the obtained cDNA.
authors.affiliation: Department of Rehabilitation Engineering, Research Institute, National Rehabilitation Center for Persons with Disabilities, Tokorozawa, Japan. kato-seishi@rehab.go.jp
authors.forename: Seishi
authors.initials: S
authors.lastname: Kato
authors.forename: Mio
authors.initials: M
authors.lastname: Oshikawa
authors.forename: Kuniyo
authors.initials: K
authors.lastname: Ohtoko
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D005822:Genetic Vectors / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger