title: Role of exopolymeric substances (EPS) in the stability of the biofilm of Thiomonas arsenivorans grown on a porous mineral support.
abstract: Biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. Biofilms of Thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (Con A or PNA), calcofluor or periodic acid (polysaccharides target), DNase (DNA target), and lipase (triglycerides target). Neither protease nor DNase treatments had any effect on bacterial adhesion. Lectins and calcofluor treatments mainly affected young biofilms. Lipase treatment had a noticeable effect on biofilm stability whatever the biofilm age. Results suggest that it would be an increased resistance of mature biofilms that protects them from external attacks.
authors.affiliation: BRGM, EPI/ECO, 3 avenue C. Guillemin, BP 36009, 45060 Orleans, Cedex 2, France. c.michel@brgm.fr
authors.forename: Caroline
authors.initials: C
authors.lastname: Michel
authors.forename: Francis
authors.initials: F
authors.lastname: Garrido
authors.forename: Emilie
authors.initials: E
authors.lastname: Roche
authors.forename: Sylvain
authors.initials: S
authors.lastname: Challan Belval
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Dictor
mesh_terms: D001557:Benzenesulfonates / Q000378:metabolism; D020562:Betaproteobacteria / Q000254:growth & development* / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D001704:Biopolymers / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004783:Environmental Microbiology; D037102:Lectins / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008903:Minerals; D010447:Peptide Hydrolases / Q000378:metabolism
chemical_list: D001557:Benzenesulfonates; D001704:Biopolymers; D037102:Lectins; D008903:Minerals; C007061:C.I. Fluorescent Brightening Agent 28; D003851:Deoxyribonucleases; D008049:Lipase; D010447:Peptide Hydrolases