title: [Characterizing effects of solvent specific morus alba components on rat platelet aggregation, vascular tension and macrophage nitrite production].
abstract: OBJECTIVE
  To study the effects of different solvent extractions of Mori Ramulus on platelet aggregation, vascular tension, and nitrite production from macrophages stimulated by lipopolysaccharides and interferon-gamma.
  
  
  METHODS
  The components of Mori Ramulus were extracted by EtoAc, n-BuOH and chloroform respectively. Platelet aggregation was induced by ADP, arachidonic acid and collagen in vitro; nitrite production of activated macrophages was measured by Griess assay, and the vasodilatory effects of three extractions were investigated by isometric tension changes of aortic rings.
  
  
  RESULTS
  Chloroform extraction concentration-dependently inhibited platelet aggregation by arachidonic acid, and reduced vascular tension of PE preconstricted aorta rings with or without endothelium. On the other hand, extractions of EtoAc and n-BuOH demonstrated dose-dependent inhibition on macrophage NO production stimulated by LPS/IFN-gamma.
  
  
  CONCLUSIONS
  Pharmacological activities of Mori Ramulus depend on solvent specific components. Chloroform extraction of Mori Ramulus may benefit cardiovascular diseases through its properties of anti-platelet aggregation and vasodilatation. The inhibition of macrophage activity by EtoAc and n-BuOH extractions suggested an anti-inflammation effect of the compound.
authors.affiliation: Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. sarah.lvlin@gmail.com
authors.forename: Shuang
authors.initials: S
authors.lastname: Ling
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhang
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.forename: Ka
authors.initials: K
authors.lastname: Bian
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000503:physiopathology; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D031625:Morus / Q000737:chemistry*; D009573:Nitrites / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D009573:Nitrites; D010975:Platelet Aggregation Inhibitors; D014665:Vasodilator Agents