title: Identification of EphA7 BAC clone containing a long-range dorsal midline-specific enhancer.
abstract: Previous studies suggest that EphA7 plays a critical role in neural tube closure or cortical progenitor apoptosis. In this report, enhancer trap assay was used to modify various EphA7 BAC clones and screen a large genomic region spanning 570 kb downstream of the EphA7 gene. We found that the dorsal midline-specific EphA7 enhancer resides on the 457D20 EphA7 BAC clone and is localized to a 35 kb genomic region in between +345.7 kb to +379.8 kb downstream of the EphA7 transcription start site. Identification of the EphA7 BAC clone containing a long-range dorsal midline enhancer may constitute a useful tool for investigating the biological functions of EphA7 in vivo.
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Korea.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Kim
authors.forename: Soochul
authors.initials: S
authors.lastname: Park
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D036141:Receptor, EphA7 / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D024363:Transcription Initiation Site
chemical_list: D011994:Recombinant Proteins; D036141:Receptor, EphA7