title: Diagnostic test of rifampicin resistance in Mycobacterium tuberculosis: a meta-analysis.
abstract: OBJECTIVE
  To evaluate available evidence on the diagnostic value of radioactivity-free cultivation and detection technologies for rapid detection of rifampicin resistance in Mycobacterium tuberculosis.
  
  
  METHODS
  A fully recursive literature search was conducted in PubMed, EMBASE, Biosis, Web of Science (all 1990-2010), CBMWeb (1978-2010), and Google Scholar. QUADAS items were used to evaluate the quality of included studies. Sensitivity, specificity, Summary receiver-operating curve SEN, SPE, SROC, and related techniques were used to assess the diagnostic value of radioactivity-free Mycobacterium tuberculosis cultivation and detection technologies.
  
  
  RESULTS
  Six studies were included in the final analysis. The MB/BacT, BACTEC MGIT 960, and Manual MGIT systems were highly sensitive and specific for detecting rifampicin-resistant TB. The summary SEN and summary SPE of the MB/BacT and BACTEC MGIT 960 systems were 100%, 99%, 100%, and 96%, respectively. The SROC of the BACTEC MGIT 960 system was 0.9943.
  
  
  CONCLUSIONS
  We recommended that the BACTEC 460 system be replaced by MB/BacT or BACTEC MGIT 960 as the final diagnostic test for rifampicin resistance in Mycobacterium tuberculosis. More studies are needed on the diagnostic value of other radioactivity-free cultivation and detection technologies to reliably determine their sensitivity and specificity for this bacterium.
authors.affiliation: Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ma
authors.forename: Guo-qin
authors.initials: GQ
authors.lastname: Qi
authors.forename: Hai-ming
authors.initials: HM
authors.lastname: Li
authors.forename: Bing-dong
authors.initials: BD
authors.lastname: Zhu
authors.forename: Ke-hu
authors.initials: KH
authors.lastname: Yang
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D003955:Diagnostic Tests, Routine; D024881:Drug Resistance, Bacterial; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D000904:Antibiotics, Antitubercular; D012293:Rifampin