title: Articular cartilage repair using an intra-articular magnet and synovium-derived cells.
abstract: The purpose of this study was to investigate the chondrogenic potential of magnetically labeled synovium-derived cells (M-SDCs) and examine whether M-SDCs could repair the articular cartilage using an intra-articular magnet after delivery to the lesion. Synovium-derived cells (SDCs) were cultured from the synovium of a rat knee, and were magnetically labeled with ferumoxides. M-SDCs were examined with a transmission electron microscope. A pellet culture system was used to evaluate the chondrogenic potential of M-SDCs in a magnetic field. In a rat model, allogeneic M-SDCs were injected into the knee after we made an osteochondral defect on the patellar groove and implanted an intra-articular magnet at the bottom of the defect. We histologically examined the defects at 48 h, 4 weeks, 8 weeks, and 12 weeks after treatment. Electron microscopy showed the transfection of ferumoxides into SDCs. The pellet cultures revealed the chondrogenic potential of M-SDCs in a magnetic field. M-SDCs accumulated in the osteochondral defect at 48 h after treatment, and we confirmed the regeneration of the articular cartilage at 4 weeks, 8 weeks, and 12 weeks after treatment using an intra-articular magnet. We demonstrated that articular cartilage defects could be repaired using an intra-articular magnet and M-SDCs. We believe that this system will be useful to repair human articular cartilage defects.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. horijun919@yahoo.co.jp
authors.forename: Junji
authors.initials: J
authors.lastname: Hori
authors.forename: Masataka
authors.initials: M
authors.lastname: Deie
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasunaga
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kawamata
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002358:Cartilage, Articular / Q000293:injuries* / Q000601:surgery; D017690:Cell Transplantation / Q000379:methods*; D003911:Dextrans; D007270:Injections, Intra-Articular; D008280:Magnetics; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D010329:Patella; D051381:Rats; D017207:Rats, Sprague-Dawley; D013264:Stifle / Q000293:injuries / Q000473:pathology* / Q000601:surgery; D013583:Synovial Membrane / Q000166:cytology* / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; C065507:ferumoxides