title: Microinjection of protein samples.
abstract: INTRODUCTIONThis protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator. Using this system, it is possible to microinject a constant volume within a 50% difference among cells.
authors.forename: Yulia
authors.initials: Y
authors.lastname: Komarova
authors.forename: John
authors.initials: J
authors.lastname: Peloquin
authors.forename: Gary
authors.initials: G
authors.lastname: Borisy