title: Electrophoretic transport of biomolecules through carbon nanotube membranes.
abstract: Electrophoretic transport of proteins across electrochemically oxidized multi-walled carbon nanotube (MWCNT) membranes has been investigated. A small charged protein, lysozyme, was successfully pumped across MWCNT membranes by an electric field while rejecting larger bovine serum albumin (BSA). Transport of lysozome was reduced by a factor of about 30 in comparison to bulk mobility and consistent with the prediction for hindered transport. Mobilities between 0.33 and 1.4 × 10(-9) m(2) V(-1) s(-1) were observed and are approximately 10-fold faster than comparable ordered nanoporous membranes and consistent with continuum models. For mixtures of BSA and lysozyme, complete rejection of BSA is seen with electrophoretic separations.
authors.affiliation: Department of Chemical and Materials Engineering, University of Kentucky , Lexington, Kentucky 40506, United States.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Ji
authors.initials: J
authors.lastname: Wu
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Hinds
mesh_terms: D000818:Animals; D001692:Biological Transport; D002417:Cattle; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000302:isolation & purification; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine; D009113:Muramidase