title: Koilocytes are enriched for alkaline-labile sites.
abstract: This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection.
authors.affiliation: División de Genética, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS, Monterrey, México. elvacortes@cibinmty.net
authors.forename: E I
authors.initials: EI
authors.lastname: Cortés-Gutiérrez
authors.forename: M I
authors.initials: MI
authors.lastname: Dávila-Rodríguez
authors.forename: J L
authors.initials: JL
authors.lastname: Fernández
authors.forename: C
authors.initials: C
authors.lastname: López-Fernández
authors.forename: J
authors.initials: J
authors.lastname: Gosálvez
mesh_terms: D016022:Case-Control Studies; D002467:Cell Nucleus / Q000235:genetics; D019457:Chromosome Breakage; D004249:DNA Damage; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D027383:Papillomaviridae / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000821:virology
chemical_list: D015342:DNA Probes