title: Gilvimarinus agarilyticus sp. nov., a new agar-degrading bacterium isolated from the seashore of Jeju Island.
abstract: An agarolytic bacterium, designated as strain M5c(T), was isolated from sea sand in Jeju Island, Korea. This isolate was Gram-negative, positive for catalase and oxidase, rod and motile by means of monotrichous flagella. Strain M5c(T) has translucent or dark ivory colonies, forms a dent on an agar plate under colonies, and grows in the presence of 1-12% (w/v) NaCl and at 10-37°C. This isolate hydrolyzes agar, alginic acid, carboxymethyl (CM)-cellulose and starch. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M5c(T) can be considered as a species within the genus Gilvimarinus, being most closely related to Gilvimarinus chinensis QM42(T), with a 16S rRNA gene sequence similarity of 95.6%. The major cellular fatty acids were C16:1ω7c and/or iso-C15:0 2OH (33.5%), C16:0 (26.5%) and C18:1ω7c (14.1%). The DNA G+C content was 53.8 mol%. Based on these polyphasic data, strain M5c(T) should be classified as a novel species, for which the name Gilvimarinus agarilyticus sp. nov. is proposed. The type strain for the novel species is M5c(T) (= KCTC 23325(T) = NCAIM B 02425(T)).
authors.affiliation: Microbiological Resources Center, KRIBB, Daejeon, Republic of Korea.
authors.forename: Byung-Chun
authors.initials: BC
authors.lastname: Kim
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Kim
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
authors.forename: Hyun Soon
authors.initials: HS
authors.lastname: Kim
authors.forename: Sung Ran
authors.initials: SR
authors.lastname: Min
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
mesh_terms: D000362:Agar / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D041965:Flavobacteriaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D000362:Agar