title: [The effect of 1,25(OH)D3 deficiency in the secondary dentin formation and mineralization and caries of the mice].
abstract: OBJECTIVE
  To determine the role of 1,25(OH)2D3 on the secondary dentin formation and mineralization of the mice.
  
  
  METHODS
  The differences of the mandible mineralization between the wild-type and 1-alpha-hydroxylase gene knockout mice at 6 weeks old were assessed by hematoxylin-eosin (HE) staining, immunohistochemistry, alkaline phosphatase (ALP) histochemistry staining.
  
  
  RESULTS
  The ratio of caries were increased significantly, while the secondary dentin was reduced significantly, the deposition of type I collagen and osteocalcin on the secondary dentin of occlusion surface was decreased significantly, but the deposition of the Biglycan on the dentin was increased significantly, the active of ALP on the odontoblasts were reduced significantly in 1-alpha-hydroxylase gene knockout mice compared to that in the wild-type littermates.
  
  
  CONCLUSIONS
  1,25(OH)2D3 deficiency lead to a defect in the secondary dentin formation and mineralization and caries of the mice.
authors.affiliation: Dept. of Medical Technology, Yancheng Health Vocational and Technical College, Yancheng 224005, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Liu-Cai
authors.initials: LC
authors.lastname: Yang
authors.forename: Deng-Shun
authors.initials: DS
authors.lastname: Miao
mesh_terms: D000818:Animals; D003804:Dentin; D003809:Dentin, Secondary; D008334:Mandible; D051379:Mice; D015675:Osteocalcin
chemical_list: D015675:Osteocalcin