title: Effects of retinoic acid and butyric acid on the expression of prestin and Gata-3 in organotypic cultures of the organ of corti of newborn rats.
abstract: Prestin is the motor protein of the outer hair cells of the organ of Corti and a key factor in ensuring a high level of sensitivity of mammalian hearing. The factors that influence prestin expression are still largely unknown. We studied the effects of the application of retinoic acid, a ligand of a nuclear receptor, and of butyric acid, an inhibitor of histone deacetylase activity, on the expression of mRNA of prestin and Gata-3 in the organotypic culture of the organ of Corti of newborn rats using RT-PCR. Application of retinoic acid at concentrations of 1-50 μM results in a dose-dependent expression decrease after two days in culture. Treatment with sodium butyrate (0.5-2 mM) elevated the expression of prestin and Gata-3. Statistically significant correlations between Gata-3 and prestin mRNA levels were observed under all conditions. The data indicate that retinoid nuclear transcription factors, GATA-3 and histone acetylation/deacetylation processes may have a regulatory role to play in prestin expression.
authors.affiliation: Department of Otorhinolaryngology, Molecular Biology Research Laboratory, Charité-Universitätsmedizin Berlin, 10117 Berlin, Charitéplatz 1, Germany. johann.gross@charite.de
authors.forename: Johann
authors.initials: J
authors.lastname: Gross
authors.forename: Kerstin
authors.initials: K
authors.lastname: Stute
authors.forename: Julia
authors.initials: J
authors.lastname: Fuchs
authors.forename: Maximilian
authors.initials: M
authors.lastname: Angerstein
authors.forename: Nyamaa
authors.initials: N
authors.lastname: Amarjargal
authors.forename: Birgit
authors.initials: B
authors.lastname: Mazurek
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D027321:Anion Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D020148:Butyric Acid / Q000378:metabolism* / Q000494:pharmacology; D050990:GATA3 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000235:genetics*; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D009925:Organ of Corti / Q000187:drug effects / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076988:Sulfate Transporters; D014212:Tretinoin / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D027321:Anion Transport Proteins; D050990:GATA3 Transcription Factor; C409207:Slc26a5 protein, rat; D000076988:Sulfate Transporters; D020148:Butyric Acid; D014212:Tretinoin