title: Diagnosis of intraocular lymphosarcoma in a dog by use of a polymerase chain reaction assay for antigen receptor rearrangement.
abstract: METHODS
  A 12-year-old castrated male mixed-breed dog was evaluated because of blepharospasm and blindness affecting both eyes.
  
  
  RESULTS
  During examination and diagnostic testing of the dog, fine-needle aspirates of splenic nodules were examined microscopically and stage Vb multicentric large-cell lymphosarcoma was identified. Aqueocentesis was performed, and sample analysis revealed intraocular lymphosarcoma; B-cell neoplasia was confirmed by use of a PCR assay for antigen receptor rearrangement (PARR) performed on samples of aqueous humor. Secondary uveitis and glaucoma were detected bilaterally in addition to chronic superficial corneal ulcerations in the left eye.
  
  
  RESULTS
  Treatment for abdominal and intraocular lymphosarcoma involving administration of vincristine, l-asparaginase, cyclophosphamide, doxorubicin, and prednisone was initiated. Secondary uveitis and glaucoma were controlled with topical treatment; however, the corneal ulceration did not resolve. Seven weeks following diagnosis, the dog died as a result of complications related to systemic neoplasia and chemotherapy.
  
  
  CONCLUSIONS
  In the dog of this report, intraocular lymphosarcoma was diagnosed via PARR performed on samples of aqueous humor. Moreover, the immunophenotype of the neoplastic cells was determined by use of that diagnostic technique. Because secondary uveitis is a common finding in dogs and cats with systemic lymphosarcoma, intraocular lymphosarcoma should be considered as a differential diagnosis; furthermore, investigation (eg, PARR performed on aqueous humor samples) to identify the presence of intraocular lymphosarcoma is warranted, thereby allowing targeted interventions to be considered in management of those patients.
authors.affiliation: Department of Clinical Sciences, North Carolina State University, Raleigh, NC 27606, USA.
authors.forename: Diana O
authors.initials: DO
authors.lastname: Pate
authors.forename: Brian C
authors.initials: BC
authors.lastname: Gilger
authors.forename: Steven E
authors.initials: SE
authors.lastname: Suter
authors.forename: Alison B
authors.initials: AB
authors.lastname: Clode
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D004283:Dog Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology; D004285:Dogs; D005134:Eye Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011946:Receptors, Antigen
chemical_list: D000970:Antineoplastic Agents; D011946:Receptors, Antigen