title: [Effect of HSV-1 infection on NGF and its receptor expression in human glioma cells].
abstract: Nerve growth factor (NGF) is mainly secreted by the neuroglia cells, which can exert biological effect through its receptors on the specific target cell surface. NGF is closely related to neurocyte growth, differentiation and apoptosis. As a neurotropic virus, HSV-1 an easily lead to neurocyte, neuroglia cells death or apoptosis. In this study, the U251 human glioma cells were chosen as target cells to study the change of NGF and its receptors in the apoptosis process of HSV-1 infection. Our results showed that U251 cells were permissive to HSV-1 replication. In the apoptosis process of HSV-1 infected U251 cells, the expression of both NGF and P75NTR increased and then decreased, while the expression of TrkA decreased gradually. These result indicated that HSV-1 was able to induce the abnormal expression of NGF and its receptors in U251 cells.
authors.affiliation: Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, China. houy20082008@163.com
authors.forename: Yun
authors.initials: Y
authors.lastname: Hou
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Hu
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Jiang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dong-Meng
authors.initials: DM
authors.lastname: Qian
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Xu-Xia
authors.initials: XX
authors.lastname: Song
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D015870:Gene Expression; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D006561:Herpes Simplex / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020932:Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism; D020917:Receptor, trkA / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D020800:Receptor, Nerve Growth Factor; D020932:Nerve Growth Factor; D020917:Receptor, trkA