title: Identification of BCP-20 (FBXO39) as a cancer/testis antigen from colon cancer patients by SEREX.
abstract: Cancer/Testis (CT) antigens are considered promising target molecules for immunotherapy. To identify potential CT antigens, we performed immunoscreening of a testis cDNA library with sera from colon cancer patients by SEREX. We isolated 114 positive cDNA clones comprising 90 different antigens, designated BCP-1 through BCP-90. Quantitative real-time and conventional RT-PCR analysis showed that BCP-20, -33, and -41 antigens were expressed strongly only in a normal testis and detected in 22 cases (39%), 12 cases (21%), and 17 cases (30%), respectively, from 57 colon tumors. BCP-20 was also detected in various cancer cell lines including breast, colon, hepatoma, renal, thyroid anaplastic, ovary, sarcoma, and lung. By ELISA analysis, anti-BCP-20 antibody was detected in 3 of 50 colon cancer and 1 of 24 gastric cancer patients while healthy donors were three positive (3/50). But the BCP-20 antibody levels of patients with colon cancer showed significantly higher titers than those of healthy donors. These data suggest that the BCP-20 gene is a new CT antigen and may be useful for diagnosis and immunotherapy.
authors.affiliation: Department of Biochemistry, School of Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do 626-770, Republic of Korea.
authors.forename: Myung-Ha
authors.initials: MH
authors.lastname: Song
authors.forename: Jong-Cheon
authors.initials: JC
authors.lastname: Ha
authors.forename: Sang-Mok
authors.initials: SM
authors.lastname: Lee
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
authors.forename: Sang-Yull
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D004274:DNA, Recombinant / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D044783:F-Box Proteins; D005260:Female; D015723:Gene Library; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012698:Serologic Tests; D013737:Testis / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D004274:DNA, Recombinant; D044783:F-Box Proteins; C559726:FBXO39 protein, human; D009363:Neoplasm Proteins