title: A biosensor for the determination of β-galactosidase activity: a different viewpoint on biosensors.
abstract: β-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor β-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. β-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for β-galactosidase detection was in the range of 0.0118 U mL(-1)to 0.47 U mL(-1)and a shorter response time of ∼50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of β-galactosidase activity.
authors.affiliation: Namık Kemal University, Chemistry Department, Tekirdağ, Turkey. msezginturk@hotmail.com
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinçkaya
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002623:Chemistry Techniques, Analytical; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005292:Ferricyanides / Q000737:chemistry / Q000378:metabolism*; D005690:Galactose / Q000032:analysis; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D058105:Polymerization; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D005292:Ferricyanides; C007931:hexacyanoferrate III; D005949:Glucose Oxidase; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose; C023650:aniline; D005690:Galactose