title: Evaluation of antioxidant and antigenotoxic activity of two flavonoids from Rhamnus alaternus L. (Rhamnaceae): kaempferol 3-O-β-isorhamninoside and rhamnocitrin 3-O-β-isorhamninoside.
abstract: The antioxidant activity of kaempferol 3-O-β-isorhamninoside (K3O-ir) and rhamnocitrin 3-O-β-isorhamninoside (R3O-ir), isolated from the leaves of Rhamnus alaternus L., was determined by the ability of each compound to inhibit NBT photoreduction and to scavenge the free radical ABTS(+)(.). Genotoxic and antigenotoxic activities were assessed using the SOS chromotest. At a concentration of 150 μg/assay the two compounds showed the most potent inhibitory activity against superoxide anion by respectively 80.4% and 85.6%. K3O-ir was a very potent radical scavenger with an IC(50) value of 18.75 μg/ml. Moreover, these two compounds exhibit an inhibitory activity against genotoxicity induced by nitrofurantoine and aflatoxine B1 using the SOS chromotest bacterial assay system in the presence of Escherichia coli PQ37 strain. In this study, we have also evaluated correlation between antigenotoxic and antioxidant effects of K3O-ir and R3O-ir. The highest correlation was showed with R3O-ir (r=0.999).
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Medecine Dentaire Monastir, Rue Avicenne, Monastir 5000, Tunisia.
authors.forename: Wissem
authors.initials: W
authors.lastname: Bhouri
authors.forename: Mohamed Ben
authors.initials: MB
authors.lastname: Sghaier
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Marie-Geneviève
authors.initials: MG
authors.lastname: Dijoux-Franca
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila Chekir
authors.initials: LC
authors.lastname: Ghedira
mesh_terms: D016604:Aflatoxin B1 / Q000037:antagonists & inhibitors; D000704:Analysis of Variance; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli / Q000378:metabolism; D044948:Flavonols / Q000494:pharmacology*; D044949:Kaempferols / Q000494:pharmacology*; D009153:Mutagens / Q000633:toxicity; D009582:Nitrofurantoin / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D031955:Rhamnus / Q000737:chemistry*; D013451:Sulfonic Acids; D013481:Superoxides; D014312:Trisaccharides / Q000494:pharmacology*
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D052160:Benzothiazoles; D044948:Flavonols; D044949:Kaempferols; D009153:Mutagens; D010936:Plant Extracts; D013451:Sulfonic Acids; D014312:Trisaccharides; C559263:kaempferol 3-O-isorhamninoside; D013481:Superoxides; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C006552:kaempferol; C072289:rhamnocitrin 3-O-isorhamninoside; D009582:Nitrofurantoin; D016604:Aflatoxin B1