title: Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells.
abstract: We synthesize drug-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres for image-guided combinatory epigenetic therapy in MCF-10A human mammary epithelial cells. LY294002 and Nile Red are encapsulated in microspheres for sustained drug release and fluorescence microscopic imaging. Drug-loaded microspheres target MCF-10A cells through a three-step binding process involving biotinylated antibody, streptavidin, and biotinylated microspheres. LY294002 loaded microspheres and 5-Aza-2-deoxycytidine are applied to MCF-10A cells for combinatory PI3K∕AKT inhibition and deoxyribonucleic acid (DNA) demethylation. Our study implies the technical potential of disease targeting and image-guided combinatory epigenetic therapy using drug-loaded multifunctional biodegradable PLGA microspheres.
authors.forename: Ronald X
authors.initials: RX
authors.lastname: Xu
authors.forename: Jeff S
authors.initials: JS
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Zuo
authors.forename: Rulong
authors.initials: R
authors.lastname: Shen
authors.forename: Tim H
authors.initials: TH
authors.lastname: Huang
authors.forename: Michael F
authors.initials: MF
authors.lastname: Tweedle
mesh_terms: D020341:Absorbable Implants; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D002214:Capsules; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage / Q000235:genetics*; D003692:Delayed-Action Preparations / Q000008:administration & dosage*; D044127:Epigenesis, Genetic / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000379:methods*
chemical_list: D002214:Capsules; D003692:Delayed-Action Preparations; D004247:DNA