title: [Development of a septic shock experimental model oriented at training. Application in the training of depuration techniques in the management of severe sepsis].
abstract: OBJECTIVE
  To define a septic shock experimental model that can be used in for training in the early management of septic shock, specifically by extracorporeal depuration (ECD).
  
  
  METHODS
  A case-control experimental study.
  
  
  METHODS
  Veterinary university hospital.
  
  
  METHODS
  Ten Beagle dogs (weight 12-15kg).
  
  
  METHODS
  Shock was induced using 1mg/kg Escherichia coli lipopolysaccharide (LPS) diluted in 20 mL saline infused in 10 minutes, with a subsequent follow-up at 6 hours. There was no intervention in 5 animals in order to define the natural course of the shock and 5 underwent high volume hemofiltration (HVHF, 100mL/kg/h) to define delay in response to treatment.
  
  
  METHODS
  Pressures (arterial and pulmonary), hemodynamic parameters, gastric tonometry and respiratory function were recorded.
  
  
  RESULTS
  The LPS effect was evidenced at 2 minutes of the infusion and the 10 animals showed severe shock at the end of the infusion. At 2-hours, changes between treated and non-treated animals were seen in cardiac output, systolic volume variability and mucous CO(2). Mean arterial pressure was significantly different at four hours. All non-treated subjects died during the 6-hour follow-up and all the treated animals survived for this period. Based on these results, we developed a workshop that has been used in five courses (www.ccmijesususon.com - www.crrtcordoba.com.es/), obtaining the previous results.
  
  
  CONCLUSIONS
  Our shock model shows a predictable behavior, very short latency and a sufficiently rapid improvement in the treated animals for it to be applied in training workshops. It is useful for training in the high-volume hemofiltration (HVHF) and can be used for training in the early management of septic shock.
authors.affiliation: Cuidados Críticos y urgencias, Hospital Universitario Carlos Haya, Málaga, Spain. sofipacho@hotmail.com
authors.forename: M E
authors.initials: ME
authors.lastname: Herrera-Gutiérrez
authors.forename: G
authors.initials: G
authors.lastname: Seller-Pérez
authors.forename: G
authors.initials: G
authors.lastname: Quesada García
authors.forename: M M
authors.initials: MM
authors.lastname: Granados
authors.forename: J M
authors.initials: JM
authors.lastname: Domínguez
authors.forename: R J
authors.initials: RJ
authors.lastname: Gómez-Villamandos
mesh_terms: D000818:Animals; D004285:Dogs; D019446:Endotoxemia / Q000150:complications; D006439:Hemodynamics; D006440:Hemofiltration / Q000379:methods*; D055552:Infectious Disease Medicine / Q000193:education* / Q000379:methods; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity; D023421:Models, Animal; D012772:Shock, Septic / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*
chemical_list: D008070:Lipopolysaccharides; C440499:lipopolysaccharide, Escherichia coli O111 B4