title: Investigation of the effect of donor platelet endothelial cell adhesion molecule 1 polymorphism on the graft-vs.-host disease occurrence in Tunisian recipients of hematopoietic stem cells.
abstract: OBJECTIVE
  The aim of this study is to examine the effect of donor PECAM-1 alleles and haplotypes for the SNPs L98V, S536N, and R643G on the occurrence of GVHD in Tunisian recipients of HSCs.
  
  
  METHODS
  This study enrolled 102 patients and their 102 respective HLA-identical sibling donors of HSCs. The PECAM-1 SNPs genotyping assay was performed using sets of sequence specific primers (SSP-PCR).
  
  
  RESULTS
  The single marker association analysis showed that the L98 allele, in a recessive genetic model, may be a potential risk factor only for acute GVHD (p=0.036, OR=2.580, 95% C.I. = 1.053-6.326). However, the haplotype analysis showed a lack of association between donor's PECAM-1 SNPs and GVHD incidence in recipient.
  
  
  CONCLUSIONS
  The homozygosity state for donor PECAM-1L98 allele may be a significant risk factor for acute GVHD. This is probably due to its action on the function of donor leukocytes especially during the extravasation process.
authors.affiliation: The Immunogenetic applied to cells therapy Research Unit, The Immunohaematology and HLA-Typing Department, National Blood Transfusion Centre of Tunis, 1006 Tunis, Tunisia. sellamimh@laposte.net
authors.forename: Mohamed Hichem
authors.initials: MH
authors.lastname: Sellami
authors.forename: Lamia
authors.initials: L
authors.lastname: Torjemane
authors.forename: Saloua
authors.initials: S
authors.lastname: Ladeb
authors.forename: Houda
authors.initials: H
authors.lastname: Kaabi
authors.forename: Amira
authors.initials: A
authors.lastname: Ben Ahmed
authors.forename: Ghaya
authors.initials: G
authors.lastname: Cherif
authors.forename: Brahim
authors.initials: B
authors.lastname: Midouni
authors.forename: Tarek
authors.initials: T
authors.lastname: Ben Othmane
authors.forename: Slama
authors.initials: S
authors.lastname: Hmida
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006086:Graft vs Host Disease / Q000209:etiology*; D006239:Haplotypes / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors; D014019:Tissue Donors; D014416:Tunisia; D055815:Young Adult
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1