title: Lanthanum(III) impacts on metallothionein MTT1 and MTT2 from Tetrahymena thermophila.
abstract: Metallothionein MTT1 and MTT2 from Tetrahymena thermophila are sulfydryl-rich proteins that can bind to and are inducible by heavy metals such as mercury, cadmium, zinc, and copper. However, little is known about the induction and binding of T. thermophila metallothionein by trivalent metals. In this study, we found that 10-80 μM La(3+) can promote Tetrahymena cells proliferation, and fluorescence spectrum analysis showed that La(3+) can enter T. thermophila cells. Real-time quantitative polymerase chain reaction showed La(3+) induced the expression of MTT1 and MTT2. Furthermore, Fluorescence analysis indicated La(3+) bind to MTT1 and MTT2. These results implied that La(3+) could interact with MTT1 and MTT2 via aspartic or glutamic acid oxygen atoms.
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, 92 Wucheng Rd, Taiyuan, 030006, People's Republic of China.
authors.forename: Qinglu
authors.initials: Q
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Yajing
authors.initials: Y
authors.lastname: Zhu
authors.forename: Baofeng
authors.initials: B
authors.lastname: Chai
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007811:Lanthanum / Q000494:pharmacology*; D008668:Metallothionein / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence; D016808:Tetrahymena thermophila / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D020033:Protein Isoforms; D007811:Lanthanum; D008668:Metallothionein