title: The RNA-DNA hybrid structure determined by EPR, CD and RNase H1.
abstract: A or B: RNA-DNA hybrids, key intermediates in gene regulation, were classified by pulsed electron-electron double resonance (PELDOR) in combination with CD spectroscopy into two classes, interpreted as A- and B-like structures. RNase H1 cleavage of these hybrids is in full agreement with these assignments, cleaving the hybrids with A-like geometry preferentially. This combined analytical approach allows the interpretation and eventually the design of more easily cleavable hybrids as needed for the antisense technology.
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Max-von-Laue-Str 7, 60438 Frankfurt am Main, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Romainczyk
authors.forename: Burkhard
authors.initials: B
authors.lastname: Endeward
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Prisner
authors.forename: Joachim W
authors.initials: JW
authors.lastname: Engels
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism*; D013057:Spectrum Analysis
chemical_list: D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA; D016914:Ribonuclease H; C078856:ribonuclease HI