title: [Detection of single nucleotide polymorphisms of mthfr and dpyd genes in leukemia cell lines K562 and K562/A02].
abstract: This study was purposed to detect single nucleotide polymorphisms (SNP) of 2 pharmacokinetics-related genes in K562 and K562/A02 cell lines. Leukemia cell line K562 and its resistant line K562/A02 were cultured, the genomic DNA was isolated by QIAamp DNA Blood Mini kit, primers were designed, the related DNA fragments were amplified by PCR. The SNP genotyping of mthfr gene rs1801131, rs1801133 and rs2274976 and dpyd gene rs1801159, rs1801160 and rs17376848 was performed by means of matrix assisted laser desorption ionization-time of flight mass spectrometry method (MALDI-TOFMS). The results showed that the genotype of mthfr gene locus 1801131 was AC, rs1801133 was CC, rs2274976 was GG, genotype of dpyd gene locus 1801159 was GG, rs1801160 was GG, rs17376848 was AA in both K562 and K562/A02 cell lines. It is concluded that the above-mentioned loci of mthfr and dpyd genes in K562 and K562/A02 cell lines are not expressed differently.
authors.affiliation: Department of Hematology, Zhongda Hospital, Southeast University Medical College, Nanjing 210009, Jiangsu Province, China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Wen
authors.initials: W
authors.lastname: Bao
authors.forename: Yue-Jiao
authors.initials: YJ
authors.lastname: Zhong
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Xia
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Xu
authors.forename: Miao-Xin
authors.initials: MX
authors.lastname: Peng
mesh_terms: D004252:DNA Mutational Analysis; D017931:DNA Primers; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D005838:Genotype; D006801:Humans; D020014:K562 Cells; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D042943:Dihydrouracil Dehydrogenase (NADP); C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)