title: Probiotic inhibits the cytopathic effect induced by Escherichia coli O157:H7 in Vero cell line model.
abstract: OBJECTIVE
  The effectiveness of four strains of Bifidobacteria against enterohemorrhagic Escherichia coli O157:H7 infection was studied using a Vero cell model.
  
  
  RESULTS
  E. coli O157 was inoculated on the Vero cell line before and after treatment with probiotic. The cytopathic effect (CPE) was evaluated during 24 h of incubation. The results indicated that Shiga toxin activity was inhibited by the probiotic. To prevent a Stx2 CPE, the probiotic needs one log more than the Stx1.
  
  
  CONCLUSIONS
  The Vero cell assay, in particular, is a good model to evaluate the effect of Bifidobacteria inhibiting bacterial attachment because of soluble substances and the competitive aspect and could be used in a variety of foods like milk and yoghurt to protect pathogen bacteria.
  
  
  CONCLUSIONS
  Probiotics could control pathogenic bacteria and Vero cell introduce as a model for evaluation of probiotics against pathogen bacteria.
authors.affiliation: Razi Vaccine and Serum Research Institute, Shiraz, Iran  Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran. yahyatahamtan@yahoo.com
authors.forename: Y
authors.initials: Y
authors.lastname: Tahamtan
authors.forename: M
authors.initials: M
authors.lastname: Kargar
authors.forename: N
authors.initials: N
authors.lastname: Namdar
authors.forename: A
authors.initials: A
authors.lastname: Rahimian
authors.forename: M
authors.initials: M
authors.lastname: Hayati
authors.forename: M M
authors.initials: MM
authors.lastname: Namavari
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157; D056265:Microbial Interactions; D019936:Probiotics; D022622:Shiga Toxin 1 / Q000633:toxicity; D022641:Shiga Toxin 2 / Q000633:toxicity; D014709:Vero Cells
chemical_list: D022622:Shiga Toxin 1; D022641:Shiga Toxin 2