title: A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples.
abstract: Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPARα), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPARα-RXRα. It can be determined indirectly by immobilizing PPARα-RXRα-PFOS complex to another plate coated with PPARα antibody and subsequent measuring the level of PPARα-RXRα by using biotin-modified PPARα-RXRα probes-quantum dots-streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5 ng L(-1) with linear range between 2.5 ng L(-1) and 75 ng L(-1). Detection results of environmental water samples were highly consistent between the bioassay and HPLC/MS.
authors.affiliation: Minister of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430030, PR China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanjian
authors.initials: Y
authors.lastname: Wan
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Qiongfang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shunqing
authors.initials: S
authors.lastname: Xu
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.forename: Baihua
authors.initials: B
authors.lastname: Shu
mesh_terms: D017738:Alkanesulfonic Acids / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D005466:Fluorocarbons / Q000032:analysis* / Q000737:chemistry; D005618:Fresh Water / Q000032:analysis; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D047493:PPAR alpha; D045663:Quantum Dots; D047490:Retinoid X Receptor alpha; D019809:Streptavidin; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D007202:Indicators and Reagents; D047493:PPAR alpha; D047490:Retinoid X Receptor alpha; D014874:Water Pollutants, Chemical; D019809:Streptavidin; C076994:perfluorooctane sulfonic acid