title: Identification of zoonotic Cryptosporidium and Giardia genotypes infecting animals in Sydney's water catchments.
abstract: To identify the animal sources for Cryptosporidium and Giardia contamination, we genotyped Cryptosporidium and Giardia spp. in wildlife from Sydney's water catchments using sequence analysis at the 18S rRNA locus for Cryptosporidium and 18S rRNA and glutamate dehydrogenase (gdh) for Giardia. A total of 564 faecal samples from 16 different host species were analysed. Cryptosporidium was identified in 8.5% (48/564) samples from eight host species and Giardia was identified in 13.8% (78/564) from seven host species. Eight species/genotypes of Cryptosporidium were identified. Five G. duodenalis assemblages were detected including the zoonotic assemblages A and B.
authors.affiliation: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch 6150, Australia.
authors.forename: Josephine
authors.initials: J
authors.lastname: Ng
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.forename: Vicky
authors.initials: V
authors.lastname: Whiffin
authors.forename: Peter
authors.initials: P
authors.lastname: Cox
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005618:Fresh Water / Q000469:parasitology*; D005838:Genotype; D005872:Giardia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000235:genetics; D006801:Humans; D009517:New South Wales; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014881:Water Supply; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D005969:Glutamate Dehydrogenase