title: A novel capillary electrophoretic method for determining aliphatic aldehydes in food samples using 2-thiobarbituric acid derivatization.
abstract: A novel electrophoretic method for sensitive determination of nine aldehydes, including formaldehyde (C1), acetaldehyde (C2), propanal (C3), butanal (C4), pentanal (C5), hexanal (C6), glutaradehyde (Gla), 2,3-butanedione (Bud) and methylgloxal (MGo) in food samples, has been developed based on CE with amperometric detection (CE-AD). After being derivatized with an electroactive compound, 2-thiobarbituric acid (TBA), these nine non-electroactive aldehydes were converted to electroactive adducts, and therefore detectable by CE-AD approach. Experimental conditions of derivatization and CE-AD detection were optimized. The proposed method was validated according to International Conference on Harmonization (ICH) requirements, with recovery results ranging from 82.8 to 123.8%. Calibration plots of aliphatic aldehydes were linear (r² ≥ 0.9901) in the concentration range from 0.083 to 15.0 mg/L. The LODs were between 0.008 and 0.074 mg/L. The proposed CE-AD method provides a reliable and sensitive quantitative evaluation for non-electroactive low-molecular-mass monoaldehydes and dialdehydes in real sample matrices by employing relatively simple and inexpensive instrument.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai, P. R. China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Zhang
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Li
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Li
authors.forename: Qing-Cui
authors.initials: QC
authors.lastname: Chu
authors.forename: Jian-Nong
authors.initials: JN
authors.lastname: Ye
mesh_terms: D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D004041:Dietary Fats; D019075:Electrophoresis, Capillary / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D015203:Reproducibility of Results; D012639:Seeds; D012680:Sensitivity and Specificity; D013696:Temperature; D013858:Thiobarbiturates / Q000737:chemistry*; D014920:Wine
chemical_list: D000447:Aldehydes; D004041:Dietary Fats; D013858:Thiobarbiturates; C029684:thiobarbituric acid