title: [Construction and identification of virB2 deletion mutants of Brucella vaccine strain M5-90].
abstract: OBJECTIVE
  To construct Brucella vaccine strain M5-90 delta virB2 mutants.
  
  
  METHODS
  Suicide plasmid pGEM-7zf-delta virB2-sacB was constructed by traditional molecular biology technology. Through the method of homologous recombination, we screened Brucella gene deletion mutant by 100 mg/L ampicillin resistance screening and 5% sugar sensitivity screening after electroporation. The M5-90 delta virB2 mutant was identified by PCR. Its stability was detected by continuous bacteria culture.
  
  
  RESULTS
  The virB2 deletion mutant strain was successfully constructed. The reverse mutation did not occur within 10 passages.
  
  
  CONCLUSIONS
  The results of this study will be based on developing the new attenuated vaccine of Brucella gene deletion mutants.
authors.affiliation: College of Life Science, College of Animal Science and Technology, College of Medicine, Shihezi University, Shihezi 832003, China. lizhen630@sina.com
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Hongxing
authors.initials: H
authors.lastname: Zhang
authors.forename: Liyan
authors.initials: L
authors.lastname: Tang
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002002:Brucella / Q000235:genetics*; D002004:Brucella Vaccine; D017353:Gene Deletion; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014613:Vaccines, Attenuated
chemical_list: D002004:Brucella Vaccine; D014613:Vaccines, Attenuated