title: Use of NMR to Study H. pylori Metabolism.
abstract: Until recently, the culture of Helicobacter pylori in vitro has only been achievable with complex undefined media supplemented with blood products, such as Brain-heart infusion (BHI) medium, blood agar, chocolate agar, or lysed blood agar (1). Substrate utilization and metabolic activity during growth on these media is difficult to define; this has led to the use of techniques such as nuclear magnetic resonance (NMR) spectroscopy to investigate the metabolic pathways active in H. pylori (e.g., refs. 2-5). Defined media for the growth of H. pylori have now been described (6,7), however, and may facilitate future metabolic studies in this organism by more conventional techniques.
authors.affiliation: Exploratory Chemistry Unit, Glaxo Wellcome Research and Development, Stevenage, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Chalk
authors.forename: A D
authors.initials: AD
authors.lastname: Roberts
authors.forename: A A
authors.initials: AA
authors.lastname: Davison
authors.forename: D J
authors.initials: DJ
authors.lastname: Kelly
authors.forename: P J
authors.initials: PJ
authors.lastname: White