title: A novel downstream process for 1,3-propanediol from glycerol-based fermentation.
abstract: In this paper, a downstream process for purification of 1,3-propanediol from glycerol-based fermentation broth was investigated. The purification of 1,3-propanediol from fermentation broth was achieved by a process combining microfiltration, charcoal treatment, vacuum distillation, and silica gel chromatography. The broth was first filtered through hollow fiber cartridge, wherein 98.7% of biomass was removed. Soluble proteins and other color impurities in the broth were removed by the use of activated charcoal at optimal concentration of 30 g l(-1) where the soluble proteins in the broth decreased to 0.1 g l(-1) (96.0% protein loss). The obtained broth when concentrated by vacuum distillation resulted in the crystallization of inorganic salts. Subsequently, 1,3-propanediol was purified by gradient chromatography using silica gel as a stationary phase and mixture of chloroform and methanol as a mobile phase. Finally, with the optimal flow rate of 10 ml min(-1) and loading amount of 80 ml, the yield of 1,3-propanediol achieved was 89%. The overall yield of 1,3-propanediol using the proposed procedure was 75.47%. The developed method was found to be a simple, rapid, and efficient procedure for the purification of 1,3-propanediol from fermentation broth.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India. pinkianand@gmail.com
authors.forename: Pinki
authors.initials: P
authors.lastname: Anand
authors.forename: Rajendra Kumar
authors.initials: RK
authors.lastname: Saxena
authors.forename: Ruchi G
authors.initials: RG
authors.lastname: Marwah
mesh_terms: D002845:Chromatography / Q000379:methods*; D003013:Clostridium / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D011409:Propylene Glycols / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol