title: [Determination of the binding rate of rat plasma protein with salvianolic acid B].
abstract: This paper is aimed to report the development of a method for the determination of the binding rate of plasma protein with salvianolic acid B. In vitro, equilibrium dialysis method was used to imitate the binding process between salvianolic acid B and plasma protein, in vivo, ultrafiltration method was used and the binding rate with HPLC was determined. Plasma samples were treated with methanol to precipitate the protein, and the buffer solution was directly determined after filtering. The calibration curve of the buffer solution was linear in the range of 0.5-20 microg mL(-1). The calibration curve of the plasma was linear in the range of 2-200 microg mL(-1). The extract recovery was 68.6%-81.9%. RSDs of intra- and inter-day precisions were all less than 8.5%. The binding rates of plasma protein with salvianolic acid B in vitro was 75.2% and in vivo was 92.1%. This paper shows the high binding power of salvianolic acid B to plasma protein with high sensitivity, good reproduction, simple management and fulfilling the requirement.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Chun-jie
authors.initials: CJ
authors.lastname: Jing
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Kai-shun
authors.initials: KS
authors.lastname: Bi
authors.forename: De-an
authors.initials: DA
authors.lastname: Guo
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D008297:Male; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods; D053630:Depsides
chemical_list: D001572:Benzofurans; D001798:Blood Proteins; D004365:Drugs, Chinese Herbal; C076944:salvianolic acid B; D053630:Depsides