title: Fast statistical tests for detecting heterotachy in protein evolution.
abstract: The w statistic introduced by Lockhart et al. (1998. A covariotide model explains apparent phylogenetic structure of oxygenic photosynthetic lineages. Mol Biol Evol. 15:1183-1188) is a simple and easily calculated statistic intended to detect heterotachy by comparing amino acid substitution patterns between two monophyletic groups of protein sequences. It is defined as the difference between the fraction of varied sites in both groups and the fraction of varied sites in each group. The w test has been used to distinguish a covarion process from equal rates and rates variation across sites processes. Using simulation we show that the w test is effective for small data sets and for data sets that have low substitution rates in the groups but can have difficulties when these conditions are not met. Using site entropy as a measure of variability of a sequence site, we modify the w statistic to a w' statistic by assigning as varied in one group those sites that are actually varied in both groups but have a large entropy difference. We show that the w' test has more power to detect two kinds of heterotachy processes (covarion and bivariate rate shifts) in large and variable data. We also show that a test of Pearson's correlation of the site entropies between two monophyletic groups can be used to detect heterotachy and has more power than the w' test. Furthermore, we demonstrate that there are settings where the correlation test as well as w and w' tests do not detect heterotachy signals in data simulated under a branch length mixture model. In such cases, it is sometimes possible to detect heterotachy through subselection of appropriate taxa. Finally, we discuss the abilities of the three statistical tests to detect a fourth mode of heterotachy: lineage-specific changes in proportion of variable sites.
authors.affiliation: Department of Mathematics and Statistics, Dalhousie University, Halifax, Nova Scotia, Canada. hcwang@mathstat.dal.ca.
authors.forename: Huai-Chun
authors.initials: HC
authors.lastname: Wang
authors.forename: Edward
authors.initials: E
authors.lastname: Susko
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Roger
mesh_terms: D019943:Amino Acid Substitution; D003198:Computer Simulation; D019143:Evolution, Molecular; D015233:Models, Statistical; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins