title: Nocturnin expression is induced by fasting in the white adipose tissue of restricted fed mice.
abstract: The relationship between circadian clocks and metabolism is intimate and complex and a number of recent studies have begun to reveal previously unknown effects of food and its temporal availability on the clock and the rhythmic transcriptome of peripheral tissues. Nocturnin, a circadian deadenylase, is expressed rhythmically in a wide variety of tissues, but we report here that Nocturnin expression is arrhythmic in epididymal white adipose tissue (eWAT) of mice housed in 12:12 LD with ad libitum access to food. However, Nocturnin expression becomes rhythmic in eWAT of mice placed on restricted feeding. We show here that Nocturnin's rhythmic expression pattern is not dependent upon feeding, nor is it acutely induced by feeding in the liver or eWAT of ad libitum fed mice. However, Nocturnin is acutely induced by the absence of the expected meal in eWAT of restricted fed mice. A rise in cAMP levels also induces Nocturnin expression, suggesting that Nocturnin's induction in eWAT by fasting is likely mediated through the same pathways that activate lipolysis. Therefore, this suggests that Nocturnin plays a role in linking nutrient sensing by the circadian clock to lipid mobilization in the adipocytes.
authors.affiliation: Department of Biology, University of Virginia, Charlottesville, Virginia, United States of America.
authors.forename: Misty R
authors.initials: MR
authors.lastname: Gilbert
authors.forename: Nicholas
authors.initials: N
authors.lastname: Douris
authors.forename: Siripong
authors.initials: S
authors.lastname: Tongjai
authors.forename: Carla B
authors.initials: CB
authors.lastname: Green
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004032:Diet; D005215:Fasting / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000235:genetics*; D010507:Periodicity; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D009687:Nuclear Proteins; D014157:Transcription Factors; C102804:nocturnin; D000242:Cyclic AMP