title: NMR solution structure of a cyanovirin homolog from wheat head blight fungus.
abstract: Members of the cyanovirin-N homolog (CVNH) lectin family are found in bacteria, fungi and plants. As part of our ongoing work on CVNH structure-function studies, we determined the high-resolution NMR solution structure of the homolog from the wheat head blight disease causing ascomycetous fungus Gibberella zeae (or Fusarium graminearum), hereafter called GzCVNH. Like cyanovirin-N (CV-N), GzCVNH comprises two tandem sequence repeats and the protein sequence exhibits 30% identity with CV-N. The overall structure is similar to those of other members of the CVNH family, with the conserved pseudo-symmetric halves of the structure, domains A and B, closely resembling recently determined structures of Tuber borchii, Neurospora crassa, and Ceratopteris richardii CVNH proteins. Although GzCVNH exhibits a similar glycan recognition profile to CV-N and specifically binds to Manα(1-2)Manα, its weak carbohydrate binding affinity to only one binding site is insufficient for conferring anti-HIV activity.
authors.affiliation: Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Matei
authors.forename: John M
authors.initials: JM
authors.lastname: Louis
authors.forename: JunGoo
authors.initials: J
authors.lastname: Jee
authors.forename: Angela M
authors.initials: AM
authors.lastname: Gronenborn
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000494:pharmacology; D002352:Carrier Proteins / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D005656:Fungal Proteins / Q000737:chemistry* / Q000494:pharmacology; D005874:Gibberella / Q000737:chemistry*; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D019380:Anti-HIV Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005656:Fungal Proteins; D037102:Lectins; C107733:cyanovirin N