title: (-)-Anonaine induces DNA damage and inhibits growth and migration of human lung carcinoma h1299 cells.
abstract: The anticancer effects of (-)-anonaine were investigated in this current study. (-)-Anonaine at concentration ranges of 50-200 μM exhibited significant inhibition to cell growth and migration activities on human lung cancer H1299 cells at 24 h, albeit cell cycle analyses showed that (-)-anonaine at the above concentration ranges did not cause any significant changes in cell-cycle distributions. Significant nuclear damages of H1299 cells were observed with 10-200 μM (-)-anonaine treatment in a comet assay, whereas higher concentrations (6 and 30 mM) of (-)-anonaine concentrations were required to cause DNA damages in an in vitro plasmid cleavage assay. In summary, our results demonstrated that (-)-anonaine exhibited dose-dependent antiproliferatory, antimigratory, and DNA-damaging effects on H1299 cells. We inferred that (-)-anonaine can cause cell-cycle arrest and DNA damage to hamper the physiological behavior of cancer cells at 72 h, and therefore, it can be useful as one of the potential herbal supplements for chemoprevention of human lung cancer.
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, and Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Bing-Hung
authors.initials: BH
authors.lastname: Chen
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Hsuan-Min
authors.initials: HM
authors.lastname: Huang
authors.forename: Inn-Wen
authors.initials: IW
authors.lastname: Chong
authors.forename: Jia-Shing
authors.initials: JS
authors.lastname: Chen
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D000077192:Adenocarcinoma of Lung; D001060:Aporphines / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D004149:Dioxoles / Q000494:pharmacology*; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000503:physiopathology*
chemical_list: D001060:Aporphines; D004149:Dioxoles; C098138:anonaine