title: [ClC-3 siRNA inhibits regulatory volume decrease in nasopharyngeal carcinoma cells].
abstract: OBJECTIVE
  To investigate the role of ClC-3 chloride channels in regulatory volume decrease (RVD) of nasopharyngeal carcinoma (NPC) CNE-2Z cells.?
  
  
  METHODS
  ClC-3 siRNA was transfected into CNE-2Z cells in the presence of the transfection reagent HiPerFect Reagent(TM). The transfection efficiency of ClC-3 siRNA was detected by flow cytometry. The expression of ClC-3 protein was detected by Western blotting, and the changes of cell volume in 160 mOsmol/L hypotonic solution were determined by image analysis.
  
  
  RESULTS
  The transfection efficiency of ClC-3 siRNA was (63.8∓3.8)% (n=3, P<0.01), and compared with the control group, ClC-3 siRNA transfection resulted in a reduction of ClC-3 expression by (60.9∓4.0)% (n=3, P<0.01). The hypotonic challege (160 mOsmol/L) caused cell swelling and induced RVD. In the control group, hypotonic solution bath for 35 min resulted in a RVD of (42.6∓2.8)% (n=20), which was significantly decreased to (10.5∓4.8)% (n=16) in ClC-3 siRNA-transfected cells, demonstrating a reduction of RVD capacity by 75.4% (P<0.01).?
  
  
  CONCLUSIONS
  The capacity of RVD is significantly reduced in CNE-2Z cells by ClC-3 chloride channel protein knock-down via ClC-3 siRNA transfection, indicating an important role of ClC-3 chloride channels in the RVD of CNE-2Z cells.
authors.affiliation: Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China. yedong0601@163.com
authors.forename: Dong
authors.initials: D
authors.lastname: Ye
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Lin-yan
authors.initials: LY
authors.lastname: Zhu
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Wang
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D048429:Cell Size; D018118:Chloride Channels / Q000235:genetics*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D018118:Chloride Channels; C115376:ClC-3 channel; D034741:RNA, Small Interfering