title: Studies on target tissue distribution of ginsenosides and epimedium flavonoids in rats after intravenous administration of Jiweiling freeze-dried powder.
abstract: A simple and rapid liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for analysis of ginsenoside Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, icariin and epimedin A, B, C in rat target tissues (spinal cord, brain, muscle and sciatic nerve) after intravenous administration of Jiweiling freeze-dried powder using genistein as an internal standard (IS). The tissue samples were treated by protein precipitation with methanol prior to HPLC and chromatographic separation was performed on a C18 column utilizing a gradient elution program with acetonitrile and 0.1% formic acid aqueous. Electrospray ionization (ESI) source was employed and the 11 analytes and IS were detected by multiple reaction monitoring (MRM) scanning under the negative ionization mode. Higher sensitivity was achieved and the optimized mass transition ion-pairs (m/z) for quantitation were selected. The calibration curves were linear over the investigated concentration ranges with correlation coefficients higher than 0.995. The intra- and inter-day RSDs were all less than 10% with the relative error (RE) within ± 9.3%. The mean extraction recoveries for all compounds were between 93.3 and 106%. The proposed method was successfully applied to investigate the target tissue distribution of the 11 compounds in rat after intravenous administration of Jiweiling freeze-dried powder.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, 050017, China; Institute of Hebei Yiling Pharmaceutical Co., Ltd., Shijiazhuang, 050035, China.
authors.forename: Minyan
authors.initials: M
authors.lastname: Liu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Shi
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Honghui
authors.initials: H
authors.lastname: Xu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Li
authors.forename: Lantong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000493:pharmacokinetics*; D036145:Ginsenosides / Q000032:analysis / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014018:Tissue Distribution
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D036145:Ginsenosides