title: Genetic diversity of a Korean echovirus 5 isolate and response of the strain to five antiviral drugs.
abstract: An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it with a prototype strain (Noyce). At the nucleotide level, the P1 region (85.3%) had the highest identity value; at the amino acid level, the P3 region (98.0%) had the highest identity value. The two strains shared all cleavage sites, with the exception of the VP1/2A site, which was TY/GA in the Noyce strain but TR/GA in the Korean ECV 5 isolate. In Vero cells infected with the Korean ECV 5 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC₅₀ value >100 μg/mL. Of the five drugs, only amantadine (IC₅₀: 1 ± 0.42 μg/mL, TI: 100) and ribavirin (IC₅₀: 22 ± 1.36 μg/mL, TI: 4.55) had any antiviral activity against the Korean ECV 5 isolate.
authors.affiliation: Department of Microbiology, Chungcheongnam-Do Institute of Health and Environmental Research, Daejeon, Korea.
authors.forename: Kwisung
authors.initials: K
authors.lastname: Park
authors.forename: Jaehyoung
authors.initials: J
authors.lastname: Song
authors.forename: Kyoungah
authors.initials: K
authors.lastname: Baek
authors.forename: Changgyun
authors.initials: C
authors.lastname: Lee
authors.forename: Donguk
authors.initials: D
authors.lastname: Kim
authors.forename: Samhyun
authors.initials: S
authors.lastname: Cho
authors.forename: Joonsoo
authors.initials: J
authors.lastname: Park
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Choi
authors.forename: Byunghak
authors.initials: B
authors.lastname: Kang
authors.forename: Hwajung
authors.initials: H
authors.lastname: Choi
authors.forename: Doo-Sung
authors.initials: DS
authors.lastname: Cheon
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004457:Echovirus Infections / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D006801:Humans; D007723:Korea / Q000453:epidemiology; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins