title: The concentration of white spot disease virus for its detection in sea water using a combined ferric colloid adsorption- and foam separation-based method.
abstract: The white spot disease virus (WSDV), which is most virulent in shrimp, is a cause of serious damage in the shrimp production industry. However, it is difficult to track the infection route and behaviour of WSDV in shrimp farms because it is present at extremely low concentrations in culture sea water. In this study, the concentration of WSDV in sea water foam was examined using dispersed bubbles and milk casein as a surface-active protein. WSDV concentrations were assessed using real-time PCR. When ferric colloid adsorption was performed prior to foam separation, WSDV was effectively removed from sea water and concentrated in the generated foam within 5 min. The removal efficiency was greater than 90% at the optimum iron and casein concentrations of 1mg Fe/l and 1mg/l, respectively. Furthermore, to analyse the dissolution of the collected ferric colloid, the WSDV concentration in the colloid-dissolved solution was set to be 200-fold higher than that found in raw water. This represents a novel method of concentrating WSDV for its detection in sea water using a combination of ferric colloid adsorption and foam separation that is easy to perform, rapid and efficient.
authors.affiliation: Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, 889-2192 Miyazaki, Japan. suzuki@civil.miyazaki-u.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Takahiko
authors.initials: T
authors.lastname: Suzuki
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kono
authors.forename: Tohoru
authors.initials: T
authors.lastname: Mekata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Itami
mesh_terms: D000327:Adsorption; D000818:Animals; D005591:Chemical Fractionation / Q000379:methods; D003102:Colloids / Q000737:chemistry; D004267:DNA Viruses / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005290:Ferric Compounds / Q000737:chemistry; D033561:Penaeidae / Q000821:virology*; D016133:Polymerase Chain Reaction; D012623:Seawater / Q000821:virology*; D014773:Virology / Q000379:methods*
chemical_list: D003102:Colloids; D004279:DNA, Viral; D005290:Ferric Compounds