title: [Effects of the +83C/T polymorphism in apolipoprotein AI gene on the changes of serum biochemical traits of gluocse and lipid metabolism induced by high-carbohydrate diets].
abstract: OBJECTIVE
  The aim of the present study was to investigate the possible effects of the +83C/T polymorphism in apolipoprotein AI gene (apoA1) on the changes of serum lipids, glucose, insulin and the homeostasis model assessment of insulin resistance (HOMA-IR) induced by a high-carbohydrate diet in healthy youth.
  
  
  METHODS
  Fifty-six participants were given a washout diet for 7 d, followed by a high-carbohydrate diet for 6 d. The washout diet contained 15% protein, 31% fat and 54% carbohydrate. The high-carbohydrate diet contained 15% protein, 15% fat and 70% carbohydrate. Twelve hour fasting venous blood was drawn on the mornings of the first, eighth and fourteenth days. Blood lipids, glucose and insulin were measured, and HOMA-IR was calculated. The genome DNA was extracted and the apoA1 + 83C/T polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism.
  
  
  RESULTS
  Triglyceride and insulin were found significantly increased in the subjects with the CC genotype, but not in the T carriers after the high-carbohydrate diet. Significant decreases of total cholesterol and LDL-C and a significant increase of HDL-C were observed after the dietary intervention of the high-carbohydrate diet.
  
  
  CONCLUSIONS
  The triglyceride and insulin changes after the high-carbohydrate diet can be modulated by the apoA1 +83C/T polymorphism, and the T allele may eliminate the increase in triglyceride and insulin induced by the high-carbohydrate diet.
authors.affiliation: Department of Biochemistry and Molecular Biology Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu.
authors.forename: Yang-yan
authors.initials: YY
authors.lastname: Song
authors.forename: Ren-rong
authors.initials: RR
authors.lastname: Gong
authors.forename: Rong-hui
authors.initials: RH
authors.lastname: Li
authors.forename: Yu-jia
authors.initials: YJ
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ding-zhi
authors.initials: DZ
authors.lastname: Fang
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000235:genetics*; D001786:Blood Glucose / Q000378:metabolism*; D004032:Diet; D004040:Dietary Carbohydrates / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008297:Male; D011110:Polymorphism, Genetic; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
chemical_list: D016632:Apolipoprotein A-I; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D008055:Lipids; D014280:Triglycerides