title: Mechano Growth Factor E peptide (MGF-E), derived from an isoform of IGF-1, activates human muscle progenitor cells and induces an increase in their fusion potential at different ages.
abstract: Loss of muscle mass and strength is a major problem during aging and the expression of Mechano Growth Factor (MGF), a member of the IGF-1 (insulin-like Growth Factor 1) super family, has been shown to be both exercise and age dependent. MGF, also called IGF-1Ec, has a unique E domain with a 49bp insert in humans (52bp in rodents; IGF-1Eb), which results in a reading frame shift during the IGF-1 gene splicing to produce a distinct mature isoform. We have studied the effects of the MGF-24aa-E peptide on proliferation and differentiation of primary human muscle cell cultures isolated from healthy subjects of different ages. We found that MGF-E peptide significantly increases the proliferative life span and delays senescence of satellite cells isolated from neonatal and young adult but not from old adult muscle, hypertrophy associated with a significant decrease in the percentage of reserve cells was observed in all cultures. It is concluded that the MGF-24aa-E peptide alone has a marked ability to enhance satellite cell activation, proliferation and fusion for muscle repair and maintenance and could provide a new strategy to combat age related sarcopenia without the oncogenic side effects observed for IGF1.
authors.affiliation: Thérapie des maladies du muscle strié, Institut de Myologie, Université Pierre et Marie Curie, G.H. Pitié-Salpétrière, 47 bd de l'hôpital, Paris, France.
authors.forename: Prashanth Kumar
authors.initials: PK
authors.lastname: Kandalla
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Goldspink
authors.forename: Gillian
authors.initials: G
authors.lastname: Butler-Browne
authors.forename: Vincent
authors.initials: V
authors.lastname: Mouly
mesh_terms: D000293:Adolescent; D000367:Age Factors; D000368:Aged; D000818:Animals; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D006984:Hypertrophy; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D051381:Rats; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology; D013234:Stem Cells / Q000187:drug effects*
chemical_list: D020033:Protein Isoforms; C523997:mechano-growth factor E, human; D007334:Insulin-Like Growth Factor I; D018995:Myosin Heavy Chains