title: Regenerated silk fibroin scaffold and infrapatellar adipose stromal vascular fraction as feeder-layer: a new product for cartilage advanced therapy.
abstract: Articular cartilage has limited repair and regeneration potential, and the scarcity of treatment modalities has motivated attempts to engineer cartilage tissue constructs. The use of chondrocytes in cartilage tissue engineering has been restricted by the limited availability of these cells, their intrinsic tendency to lose their phenotype during the expansion, as well as the difficulties during the first cell adhesion to the scaffold. Aim of this work was to evaluate the intra-articular adipose stromal vascular fraction attachment on silk fibroin scaffold to promote chondrocytes adhesion and proliferation. Physicochemical characterization has demonstrated that three-dimensionally organized silk fibroin scaffold is an ideal biopolymer for cartilage tissue engineering; it allows cell attachment, scaffold colonization, and physically cell holding in the area that must be repaired; the use of adipose-derived stem cells is a promising strategy to promote adhesion and proliferation of chondrocytes to the scaffold as an autologous human feeder layer.
authors.affiliation: Dipartimento di Scienze del Farmaco, Università di Pavia, Pavia, Italia.
authors.forename: Theodora
authors.initials: T
authors.lastname: Chlapanidas
authors.forename: Silvio
authors.initials: S
authors.lastname: Faragò
authors.forename: Federica
authors.initials: F
authors.lastname: Mingotto
authors.forename: Francesca
authors.initials: F
authors.lastname: Crovato
authors.forename: Marta Cecilia
authors.initials: MC
authors.lastname: Tosca
authors.forename: Barbara
authors.initials: B
authors.lastname: Antonioli
authors.forename: Massimo
authors.initials: M
authors.lastname: Bucco
authors.forename: Giulia
authors.initials: G
authors.lastname: Lucconi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Scalise
authors.forename: Daniele
authors.initials: D
authors.lastname: Vigo
authors.forename: Massimo
authors.initials: M
authors.lastname: Faustini
authors.forename: Mario
authors.initials: M
authors.lastname: Marazzi
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Torre
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply* / Q000166:cytology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002152:Calorimetry, Differential Scanning; D002356:Cartilage / Q000473:pathology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D018920:Coculture Techniques; D005260:Female; D005349:Fibroins / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010329:Patella / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D055815:Young Adult
chemical_list: D005349:Fibroins