title: Baccatin III, a synthetic precursor of taxol, enhances MHC-restricted antigen presentation in dendritic cells.
abstract: Baccatin III, a precursor for the semisynthesis of taxol, is widely considered to be an inactive derivative of taxol. Here we show that baccatin III efficiently enhances MHC-restricted antigen presentation in dendritic cells. Baccatin III increased both class I- and class II-restricted presentation of exogenous OVA in bone marrow-derived dendritic cells (BM-DCs). Baccatin III also increased class I-restricted presentation of virus-encoded endogenous OVA in BM-DCs. Baccatin III did not affect the phagocytic activity of BM-DCs. The antigen presentation-enhancing activity of baccatin III was examined further with nanoparticles containing OVA and baccatin III. Inclusion of baccatin III to nanoparticles containing OVA greatly enhanced their capacity to induce class I-restricted OVA peptide presentation in DCs both in vitro and in vivo. Accordingly, nanoparticles containing both OVA and baccatin III were much more efficient in inducing an OVA-specific CTL response in mice compared to those containing OVA only. These results demonstrate that baccatin III exerts immunomodulatory activities in vivo as well as in vitro on the MHC-restricted antigen presentation.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, South Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lee
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Lee
authors.forename: Ki-Hyang
authors.initials: KH
authors.lastname: Kim
authors.forename: Sun-A
authors.initials: SA
authors.lastname: Im
authors.forename: Sukgil
authors.initials: S
authors.lastname: Song
authors.forename: Myung-Ku
authors.initials: MK
authors.lastname: Lee
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Kyungjae
authors.initials: K
authors.lastname: Kim
authors.forename: Chong-Kil
authors.initials: CK
authors.lastname: Lee
mesh_terms: D000470:Alkaloids / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D001854:Bone Marrow Cells / Q000378:metabolism; D002460:Cell Line; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008285:Major Histocompatibility Complex / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D053758:Nanoparticles; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D017239:Paclitaxel / Q000276:immunology / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects; D043823:Taxoids / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007155:Immunologic Factors; D043823:Taxoids; C073950:baccatin III; D010047:Ovalbumin; D017239:Paclitaxel