title: Analysis of multiple growth regulatory proteins using dissociable staining antibody arrays on solid tumor biopsy specimens.
abstract: Growth of tumor cells is often a function of deregulated growth factor receptors and their corresponding intracellular signalling molecules. The dissociable antibody staining arrays have the versatility to rapidly identify the expression, activation, and localization of such molecules and pathways in biopsy specimens. This report describes a protocol to quantify the activity of a panel of signalling molecules in Wilms tumor biopsy specimens and surrounding nonmalignant renal cells. We propose that this technique can be used to rapidly identify multiple markers and may aid in the study of aberrant growth regulatory mechanisms and potential targets for therapeutics from pathologic specimens.
authors.affiliation: Hughes' Children's Cancer Research Centre, Division of Pediatric Oncology, Alberta Children's Hospital, Calgary, Alberta, Canada.
authors.forename: Christopher
authors.initials: C
authors.lastname: Blackmore
authors.forename: Aarthi
authors.initials: A
authors.lastname: Jayanthan
authors.forename: Eva
authors.initials: E
authors.lastname: Ujack
authors.forename: Tony
authors.initials: T
authors.lastname: Magliocco
authors.forename: Aru
authors.initials: A
authors.lastname: Narendran
mesh_terms: D001706:Biopsy; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis*; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis; D007680:Kidney Neoplasms / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis; D013194:Staining and Labeling; D046888:Tissue Array Analysis / Q000379:methods*; D009396:Wilms Tumor / Q000737:chemistry*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D020794:Receptor Protein-Tyrosine Kinases