title: Mosquito infestation and dengue virus infection in Aedes aegypti females in schools in Merida, Mexico.
abstract: We determined abundance of Aedes aegypti mosquitoes and presence of dengue virus (DENV) in females collected from schools in Mérida, México, during 2008 and 2009. Backpack aspiration from 24 schools produced 468 females of Ae. aegypti and 1,676 females of another human biter, Culex quinquefasciatus. Ae. aegypti females were collected most commonly from classrooms followed by offices and bathrooms. Of these females, 24.7% were freshly fed. Examination of 118 pools of Ae. aegypti females (total of 415 females) for presence of DENV RNA produced 19 positive pools (16.1%). DENV-infected pools were detected from 11 (45.8%) of 24 schools and came from different room types, including classrooms, offices, and bathrooms. The overall rate of DENV infection per 100 Ae. aegypti females was 4.8. We conclude that schools in Mérida present a risk environment for students, teachers, and other personnel to be exposed to mosquitoes and bites of DENV-infected Ae. aegypti females.
authors.affiliation: Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Centro, Mérida, Yucatán, México. grejon@tunku.uady.mx
authors.forename: Julián E
authors.initials: JE
authors.lastname: García-Rejón
authors.forename: María Alba
authors.initials: MA
authors.lastname: Loroño-Pino
authors.forename: José Arturo
authors.initials: JA
authors.lastname: Farfán-Ale
authors.forename: Luis F
authors.initials: LF
authors.lastname: Flores-Flores
authors.forename: Mildred P
authors.initials: MP
authors.lastname: López-Uribe
authors.forename: Maria Del Rosario
authors.initials: Mdel R
authors.lastname: Najera-Vazquez
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Nuñez-Ayala
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Beaty
authors.forename: Lars
authors.initials: L
authors.lastname: Eisen
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D003465:Culex; D003715:Dengue / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D012367:RNA, Viral / Q000302:isolation & purification; D012574:Schools
chemical_list: D012367:RNA, Viral