title: Stent-induced coronary artery stenosis characterized by multimodal nonlinear optical microscopy.
abstract: We demonstrate for the first time the applicability of multimodal nonlinear optical (NLO) microscopy to the interrogation of stented coronary arteries under different diet and stent deployment conditions. Bare metal stents and Taxus drug-eluting stents (DES) were placed in coronary arteries of Ossabaw pigs of control and atherogenic diet groups. Multimodal NLO imaging was performed to inspect changes in arterial structures and compositions after stenting. Sum frequency generation, one of the multimodalities, was used for the quantitative analysis of collagen content in the peristent and in-stent artery segments of both pig groups. Atherogenic diet increased lipid and collagen in peristent segments. In-stent segments showed decreased collagen expression in neointima compared to media. Deployment of DES in atheromatous arteries inhibited collagen expression in the arterial media.
authors.affiliation: Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, USA.
authors.forename: Han-Wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Vlad
authors.initials: V
authors.lastname: Simianu
authors.forename: Mattew J
authors.initials: MJ
authors.lastname: Locker
authors.forename: Ji-Xin
authors.initials: JX
authors.lastname: Cheng
authors.forename: Michael
authors.initials: M
authors.lastname: Sturek
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis / Q000009:adverse effects*; D023921:Coronary Stenosis / Q000209:etiology* / Q000473:pathology*; D007089:Image Enhancement / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D017711:Nonlinear Dynamics; D015607:Stents / Q000009:adverse effects*; D013552:Swine