title: SNP Mining from Maize 454 EST Sequences.
abstract: INTRODUCTIONIn this protocol, 454 expressed sequence tags (ESTs) are generated by sequencing shoot apical meristem (SAM) cDNA from maize inbred lines on the 454 Life Sciences GS-20 sequencing system. The computational tool PolyBayes (Marth et al. 1999) is then used to identify single-nucleotide polymorphisms (SNPs). PolyBayes has been used successfully to identify SNPs in many different systems, including maize, and is particularly recommended for identifying SNPs in 454 sequences.
authors.affiliation: Donald Danforth Plant Science Center, St. Louis, MO 63132, USA.
authors.forename: W Brad
authors.initials: WB
authors.lastname: Barbazuk
authors.forename: Scott
authors.initials: S
authors.lastname: Emrich
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Schnable