title: Lateral dynamics of proteins with polybasic domain on anionic membranes: a dynamic Monte-Carlo study.
abstract: Positively charged polybasic domains are essential for recruiting multiple signaling proteins, such as Ras GTPases and Src kinase, to the negatively charged cellular membranes. Much less, however, is known about the influence of electrostatic interactions on the lateral dynamics of these proteins. We developed a dynamic Monte-Carlo automaton that faithfully simulates lateral diffusion of the adsorbed positively charged oligopeptides as well as the dynamics of mono- (phosphatidylserine) and polyvalent (PIP(2)) anionic lipids within the bilayer. In agreement with earlier results, our simulations reveal lipid demixing that leads to the formation of a lipid shell associated with the peptide. The computed association times and average numbers of bound lipids demonstrate that tetravalent PIP(2) interacts with the peptide much more strongly than monovalent lipid. On the spatially homogeneous membrane, the lipid shell affects the behavior of the peptide only by weakly reducing its lateral mobility. However, spatially heterogeneous distributions of monovalent lipids are found to produce peptide drift, the velocity of which is determined by the total charge of the peptide-lipid complex. We hypothesize that this predicted phenomenon may affect the spatial distribution of proteins with polybasic domains in the context of cell-signaling events that alter the local density of monovalent anionic lipids.
authors.affiliation: Centre for Systems Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
authors.forename: Vladimir Yu
authors.initials: VY
authors.lastname: Kiselev
authors.forename: Davide
authors.initials: D
authors.lastname: Marenduzzo
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Goryachev
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D004058:Diffusion; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D009010:Monte Carlo Method; D057786:Peptidomimetics / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D057786:Peptidomimetics; D011506:Proteins