title: Antioxidant potential of n-butanol fraction from extract of Jasminum mesnyi Hance leaves.
abstract: Methanolic extract of Jasminum mesnyi Hance leaves having antidiabetic activity was subjected to fractionation to obtain antioxidant and antihyperglycemic rich fraction. Different concentrations of ethyl acetate and n-butanol fractions were subjected to antioxidant assay by DPPH method, nitric oxide scavenging activity and reducing power assay. The fractions showed dose dependent free radical scavenging property in all the models. IC50 values for ethyl acetate and n-butanol fractions were 153.45 +/- 6.65 and 6.22 +/- 0.25 microg/ml, respectively, as compared to L-ascorbic acid and rutin (as standards; IC50 values 6.54 +/- 0.24 and 5.43 +/- 0.21 microg/ml, respectively) in DPPH model. In nitric oxide scavenging activity, IC50 values were 141.54 +/- 9.95 microg/ml, 35.12 +/- 1.58 microg/ml, 21.06 +/- 0.95 microg/ml and 29.93 +/- 0.32 microg/ml for ethyl acetate, n-butanol fractions, L-ascorbic acid and rutin, respectively. n-Butanol fraction showed a good reducing potential and better free radical scavenging activity as compared to ethyl acetate fraction. Potent antioxidant n-butanol fraction showed better oral glucose tolerance test (antihyperglycemic) at par with metformin (standard drug), n-Butanol fraction contained secoiridoid glycosides which might be responsible for both antioxidant and antihyperglycemic activity.
authors.affiliation: Department of Pharmacognosy, I S F College of Pharmacy, Moga 142 001, India.
authors.forename: Sakshi
authors.initials: S
authors.lastname: Borar
authors.forename: Priyanka
authors.initials: P
authors.lastname: Punia
authors.forename: A N
authors.initials: AN
authors.lastname: Kalia
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D000085:Acetates; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy; D016166:Free Radical Scavengers / Q000494:pharmacology; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D020128:Inhibitory Concentration 50; D031660:Jasminum / Q000737:chemistry*; D000432:Methanol; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards
chemical_list: D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D001786:Blood Glucose; D016166:Free Radical Scavengers; D007004:Hypoglycemic Agents; D010851:Picrates; D010936:Plant Extracts; D009569:Nitric Oxide; C007650:ethyl acetate; D020001:1-Butanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol