title: Neutravidin coated surfaces for single DNA molecule analysis.
abstract: We present a novel approach for single DNA molecule analysis using neutravidin coated surfaces. DNA molecules are elongated and reversibly immobilized on neutravidin coated surfaces with pH and salt controls. We demonstrate restriction enzyme reactions for optical mapping and ligation for tethered DNA molecules.
authors.affiliation: Department of Chemistry & Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, Korea.
authors.forename: Yoori
authors.initials: Y
authors.lastname: Kim
authors.forename: Kyubong
authors.initials: K
authors.lastname: Jo
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012492:Salts / Q000737:chemistry; D013499:Surface Properties
chemical_list: D012492:Salts; C093483:neutravidin; D001360:Avidin; D001710:Biotin; D004247:DNA; D004262:DNA Restriction Enzymes