title: Identification and characterization of RNF2 response elements in human kidney cells.
abstract: RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.
authors.affiliation: Graduate School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Choi
authors.forename: Seongman
authors.initials: S
authors.lastname: Kang
mesh_terms: D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005796:Genes; D017930:Genes, Reporter; D056426:Genetic Loci; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis; D063150:Polycomb Repressive Complex 1; D063146:Polycomb-Group Proteins; D034622:RNA Interference; D012097:Repressor Proteins / Q000378:metabolism; D020218:Response Elements / Q000235:genetics*; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D008156:Luciferases; D063150:Polycomb Repressive Complex 1; C489841:RNF2 protein, human; D044767:Ubiquitin-Protein Ligases