title: Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research.
abstract: MS-based investigation of pancreatic fluid enables the high-throughput identification of proteins present in the pancreatic secretome. Pancreatic fluid is a complex admixture of digestive, inflammatory, and other proteins secreted by the pancreas into the duodenum, and thus is amenable to MS-based proteomic analysis. Recent advances in endoscopic techniques, in particular the endoscopic pancreatic function test (ePFT), have improved the collection methodology of pancreatic fluid for proteomic analysis. Here, we provide an overview of MS-based proteomic techniques as applied to the study of pancreatic fluid. We address sample collection, protein extraction, MS sample preparation and analysis, and bioinformatic approaches, and summarize current MS-based investigations of pancreatic fluid. We then examine the limitations and the future potential of such technologies in the investigation of pancreatic disease. We conclude that pancreatic fluid represents a rich reservoir of potential biomarkers and that the study of the molecular mechanisms of chronic pancreatitis may benefit substantially from MS-based proteomics.
authors.affiliation: Center for Pancreatic Disease, Division of Gastroenterology, Hepatology and Endoscopy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Joao A
authors.initials: JA
authors.lastname: Paulo
authors.forename: Linda S
authors.initials: LS
authors.lastname: Lee
authors.forename: Bechien
authors.initials: B
authors.lastname: Wu
authors.forename: Peter A
authors.initials: PA
authors.lastname: Banks
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.forename: Darwin L
authors.initials: DL
authors.lastname: Conwell
mesh_terms: D002908:Chronic Disease; D016145:Endoscopy, Digestive System; D006801:Humans; D013058:Mass Spectrometry; D010186:Pancreatic Function Tests; D010189:Pancreatic Juice / Q000737:chemistry*; D010195:Pancreatitis / Q000175:diagnosis* / Q000378:metabolism* / Q000601:surgery; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins