title: Identification of phosphorylation sites on extracellular corneal epithelial cell maspin.
abstract: Maspin, a 42-kDa non-classical serine protease inhibitor (serpin), is expressed by epithelial cells of various tissues including the cornea. The protein localizes to the nucleus and cytosol, and is present in the extracellular space. While extracellular maspin regulates corneal stromal fibroblast adhesion and inhibits angiogenesis during wound healing in the cornea, the molecular mechanism of its extracellular functions is unclear. We hypothesized that identifying post-translational modifications of maspin, such as phosphorylation, may help decipher its mode of action. The focus of this study was on the identification of phosphorylation sites on extracellular maspin, since the extracellular form of the molecule is implicated in several functions. Multi-stage fragmentation MS was used to identify sites of phosphorylation on extracellular corneal epithelial cell maspin. A total of eight serine and threonine phosphorylation sites (Thr50, Ser97, Thr118, Thr157, Ser240, Ser298, Thr310 and Ser316) were identified on the extracellular forms of the molecule. Phosphorylation of tyrosine residues was not detected on extracellular maspin from corneal epithelial cell, in contrast to breast epithelial cells. This study provides the basis for further investigation into the functional role of phosphorylation of corneal epithelial maspin.
authors.affiliation: Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Malathi
authors.initials: M
authors.lastname: Narayan
authors.forename: Shama P
authors.initials: SP
authors.lastname: Mirza
authors.forename: Sally S
authors.initials: SS
authors.lastname: Twining
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D019573:Epithelium, Corneal / Q000737:chemistry* / Q000378:metabolism; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D007525:Isoelectric Focusing; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D015843:Serpins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
chemical_list: C085344:SERPIN-B5; D015843:Serpins