title: Development of a CE-ESI-microTOF-MS method for a rapid identification of phenolic compounds in buckwheat.
abstract: A sensitive CE-ESI-MS analytical method for the identification of buckwheat antioxidants has been developed. CE and ESI-TOF parameters (e.g. buffer composition and pH, sheath liquid composition, sheath liquid and gas flow rates, electrospray voltage) were optimized to obtain an optimal analytical separation and identification. The results confirmed the presence of phenolic acids, procyanidins and galloylated propelargonidins. The identification of swertiamacroside and 2-hydroxy-3-O-β-D-glucopyranosil-benzoic acid, found for the first time in our previous work, has been confirmed. Furthermore, 5,7,4'-trimethoxyflavan and dihydroxy-trimethoxyisoflavan have also been tentatively identified for the first time in buckwheat.
authors.affiliation: Dipartimento di Scienze degli Alimenti, Università di Bologna, Cesena (FC) Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Verardo
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Gomez-Caravaca
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
authors.forename: Maria Fiorenza
authors.initials: MF
authors.lastname: Caboni
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernández-Gutiérrez
mesh_terms: D000975:Antioxidants / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D000432:Methanol; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D000432:Methanol