title: The influence of microgravity on invasive growth in Saccharomyces cerevisiae.
abstract: This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Σ1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Σ1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Σ1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.
authors.affiliation: Centre for Malting and Brewing, Katholieke Universiteit Leuven, Heverlee, Belgium.
authors.forename: Sebastiaan E
authors.initials: SE
authors.lastname: Van Mulders
authors.forename: Catherine
authors.initials: C
authors.lastname: Stassen
authors.forename: Luk
authors.initials: L
authors.lastname: Daenen
authors.forename: Bart
authors.initials: B
authors.lastname: Devreese
authors.forename: Verena
authors.initials: V
authors.lastname: Siewers
authors.forename: Rudy G E
authors.initials: RG
authors.lastname: van Eijsden
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
authors.forename: Freddy R
authors.initials: FR
authors.lastname: Delvaux
authors.forename: Ronnie
authors.initials: R
authors.lastname: Willaert
mesh_terms: D015169:Colony Count, Microbial; D015180:Electrophoresis, Gel, Two-Dimensional; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013026:Space Flight; D013696:Temperature; D013997:Time Factors; D014893:Weightlessness
chemical_list: D029701:Saccharomyces cerevisiae Proteins