title: Inhibitory effect of leptin on rosiglitazone-induced differentiation of primary adipocytes prepared from TallyHO/Jng mice.
abstract: The effects of leptin on rosiglitazone-induced adipocyte differentiation were investigated in the primary adipocytes prepared from subcutaneous fat of TallyHO/Jng (TallyHO) mouse, a recently developed model animal for type 2 diabetes mellitus (T2DM). The treatment of leptin inhibited the rosiglitazone-induced adipocyte differentiation with a decreased expression of peroxisome proliferator-activated receptor γ (PPARγ) a key adipogenic transcription factor, both in mRNA and protein levels. Leptin (10 nM) was sufficient to inhibit the adipocyte differentiation, which seemed to come from increased expression of leptin receptor genes in the fat of TallyHO mice. The inhibition of adipogenesis by leptin was restored by the treatment of inhibitors for extracellular-signal-regulated kinase (ERK) (PD98059) and signal transducer and activator of transcription-1 (STAT1) (fludarabine). Furthermore, in vivo intraperitoneal administration of PD98059 and fludarabine increased the PPARγ expression in the subcutaneous fat of TallyHO mice. These data suggest that leptin could inhibit the PPARγ expression and adipocyte differentiation in its physiological concentration in TallyHO mice.
authors.affiliation: Medicinal Science Division, Korea Research Institute of Chemical Technology, 100 Jang-dong, Yuseong, 305-600 Daejon, Republic of Korea.
authors.forename: Ki Young
authors.initials: KY
authors.lastname: Kim
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Sung
authors.forename: Won Hoon
authors.initials: WH
authors.lastname: Jung
authors.forename: Hee-Youn
authors.initials: HY
authors.lastname: Kim
authors.forename: Ji Seon
authors.initials: JS
authors.lastname: Park
authors.forename: Hyae Gyeong
authors.initials: HG
authors.lastname: Cheon
authors.forename: Sang Dal
authors.initials: SD
authors.lastname: Rhee
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D005419:Flavonoids / Q000494:pharmacology; D020738:Leptin / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D047495:PPAR gamma / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D054411:Receptors, Leptin / Q000096:biosynthesis; D000077154:Rosiglitazone; D050151:Subcutaneous Fat / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D005419:Flavonoids; D020738:Leptin; D047495:PPAR gamma; D012333:RNA, Messenger; D054411:Receptors, Leptin; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one