title: Simplified approach for quantitative digital holographic phase contrast imaging of living cells.
abstract: Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging.
authors.affiliation: University of Muenster, Center for Biomedical Optics and Photonics, Robert-Koch-Str. 45, D-48149 Muenster, GermanyUniversity of Muenster, Gastroenterological Molecular Cell Biology, Department of Medicine B, D-48149 Muenster, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Kemper
authors.forename: Angelika
authors.initials: A
authors.lastname: Vollmer
authors.forename: Christina E
authors.initials: CE
authors.lastname: Rommel
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schnekenburger
authors.forename: Gert
authors.initials: G
authors.lastname: von Bally
mesh_terms: D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D006801:Humans; D007368:Interferometry / Q000295:instrumentation*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*