title: An alternative method for determining the radiochemical purity of 99mTc-tetrofosmin.
abstract: The instant thin-layer chromatographic method of determining the radiochemical purity of (99m)Tc-tetrofosmin recommended by the manufacturer uses a mixture of acetone and dichloromethane (35:65 v/v) as the solvent and a silica gel (SG) strip as the solid phase. Currently, SG strips are not available commercially and an alternative solid phase is needed. The present study reports that silicic acid strips are an appropriate substitute for SG strips for the chromatographic method using the same solvent mixture but mixed in a reverse ratio (65:35 v/v). The 2 methods yielded similar values (within 1 SD) of radiochemical purity for (99m)Tc-tetrofosmin.
authors.affiliation: Department of Nuclear Medicine (Jb-3), Cleveland Clinic Foundation, Cleveland, Ohio, USA.
authors.forename: Kanak C
authors.initials: KC
authors.lastname: Amin
authors.forename: Sagar
authors.initials: S
authors.lastname: Patel
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Doke
authors.forename: Gopal B
authors.initials: GB
authors.lastname: Saha
mesh_terms: D000096:Acetone / Q000737:chemistry; D002855:Chromatography, Thin Layer; D006868:Hydrolysis; D008752:Methylene Chloride / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000737:chemistry*; D015609:Organotechnetium Compounds / Q000032:analysis* / Q000737:chemistry*; D011854:Radiochemistry / Q000379:methods*; D058428:Silica Gel / Q000737:chemistry; D012824:Silicic Acid / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D012997:Solvents; D012824:Silicic Acid; D000096:Acetone; C078700:technetium tc-99m tetrofosmin; D008752:Methylene Chloride; D058428:Silica Gel