title: Cdc42 GTPase-activating protein (CdGAP) interacts with the SH3D domain of Intersectin through a novel basic-rich motif.
abstract: The small GTPases Rac1 and Cdc42 are key regulators of the cytoskeleton. We have previously identified the endocytic protein Intersectin as a binding partner and regulator of Cdc42 GTPase-activating protein (CdGAP) with activity towards Rac1 and Cdc42. This interaction is mediated through the SH3D domain of Intersectin and the central domain of CdGAP, which does not contain any typical proline-rich domain or known SH3-binding motif. Here, we have characterized the Intersectin-SH3D/CdGAP interaction. We show that Intersectin-SH3D interacts directly with a small region of CdGAP highly enriched in basic residues and comprising a novel conserved xKx(K/R)K motif.
authors.affiliation: Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2.
authors.forename: Martin
authors.initials: M
authors.lastname: Primeau
authors.forename: Ali
authors.initials: A
authors.lastname: Ben Djoudi Ouadda
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lamarche-Vane
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D018909:src Homology Domains
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C115491:Arhgap31 protein, mouse; D020690:GTPase-Activating Proteins; C115919:intersectin 1