title: Modeling the effects of estradiol and progesterone on the acute phase proinflammatory axis: variability in tumor necrosis factor-α, nitric oxide, and xanthine oxidase responses to endotoxin challenge in steers.
abstract: The severity of host response in some diseases differs between sexes, and this dimorphism has been attributed to the immunomodulating effects of reproductive steroid hormones. In females, susceptibility to disease stress has been associated with reproductive status and attributed to prevailing progesterone (P4) or estrogen concentrations during different estrous cycle phases. Our objective was to clarify and define the effect of P4 or 17β-estradiol (E2) on the acute proinflammatory component of the innate immune system by administering these hormones to steers and evaluating initial and tolerance-associated concentration patterns of circulating proinflammatory immune response mediators after two consecutive lipopolysaccharide (LPS) challenges (LPS1 and LPS2, 6 d apart; 2.5 μg/kg BW, intravenously, Escherichia coli 055:B5). Plasma concentrations of the proinflammatory initiation cytokine tumor necrosis factor-α (TNF-α), nitrate+nitrite [NO(x), estimate of nitric oxide (NO) production], haptoglobin (HG; acute phase protein) and plasma xanthine oxidase activity (mediator of superoxide production) were measured. Crossbred steers (392 ± 7 kg) were fed a forage-concentrate diet (15% CP) to appetite and assigned to control (C; n = 7), P4 (n = 8), or E2 (n = 5) treatment. Jugular blood samples were obtained at 0, 1, 2, 3, 4, 7, and 24 h relative to each of the two LPS injections. For each proinflammatory biomarker, the area under the time by concentration curve (AUC) was used to evaluate and compare responses to the LPS challenge. Treatment with E2 disrupted LPS tolerance as observed in augmented plasma TNF-α (P < 0.01) and NO(x) (P < 0.01) responses to LPS2. Compared with C, P4 treatment decreased plasma NO(x) AUC after LPS2 (P < 0.05) and tended to reduce TNF-α AUC after LPS1 (P = 0.08). Plasma xanthine oxidase activity AUC was increased (P < 0.01) over C by E2 treatment after both LPS1 and LPS2. HG response to LPS1 within 24 h was not affected by any treatment. However, 6 d after LPS1 plasma HG concentration remained higher (P < 0.01) in steers treated with E2 than with C or P4. Results indicate that in cattle, P4 and E2, respectively, attenuate or amplify the response to LPS challenge at several points critical to the regulation of the progression of the proinflammatory cascade.
authors.affiliation: Bovine Functional Genomics Laboratory, US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705, USA. stanislaw.kahl@ars.usda.gov
authors.forename: S
authors.initials: S
authors.lastname: Kahl
authors.forename: T H
authors.initials: TH
authors.lastname: Elsasser
authors.forename: C-J
authors.initials: CJ
authors.lastname: Li
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002417:Cattle / Q000097:blood / Q000276:immunology*; D004731:Endotoxins / Q000276:immunology; D004926:Escherichia coli; D004958:Estradiol / Q000097:blood*; D006242:Haptoglobins / Q000032:analysis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D009569:Nitric Oxide / Q000097:blood / Q000276:immunology; D011374:Progesterone / Q000097:blood*; D012727:Sex Characteristics; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology*; D014969:Xanthine Oxidase / Q000097:blood / Q000276:immunology*
chemical_list: D004731:Endotoxins; D006242:Haptoglobins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D011374:Progesterone; D004958:Estradiol; D014969:Xanthine Oxidase