title: [Utility of Hybrisep in the diagnosis of blood stream infection].
abstract: Hybrisep is an in situ hybridization (ISH) method to detect phagocytosed bacteria in peripheral blood neutrophils and macrophages. We report 10 actual clinical cases tested using Hybrisep with new DNA probes, and the data were compared to the actual blood culture results. A normal Hybrisep strategy employs 5 DNA probes to detect the following bacterial DNA: "SA" probe for S. aureus, "SE" for S. epidermidis, "PA" for P. aeruginosa, "EF" for E. faecalis, and "EK" for E. coli, E. cloacae, and K. pneumoniae. Six newly designed DNA probes were used in this study: "GB" probe for 49 common bacteria, "SP" for S. pneumoniae, "BF" for B. fragilis, "HI" for H. influenzae, "GC" for Candida species, and "CA" for C. albicans. Three cases were positive on ISH, but all their blood cultures were negative. One case showed a positive blood culture, but was negative on ISH. In another 6 cases, both were negative. We postulated that empirical therapy of antibiotics resulted in only positive ISH. Cases only showing a positive outcome on blood culture might be due to a diminished phagocytic function during patients' severe disease conditions. In conclusion, ISH with Hybrisep has clinical advantages such as being able to defect causative pathogens even after the use of antibiotics, and facilitates more rapid identification than routinely performed bacterial cultures only.
authors.affiliation: Department of Infection Control and Laboratory Medicine, Fukushima Medical University, Fukushima 960-1295, Japan. sao-hfmu@fmu.ac.jp
authors.forename: Saori
authors.initials: S
authors.lastname: Miura
authors.forename: Noboru
authors.initials: N
authors.lastname: Ohana
authors.forename: Natsuo
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Imafuku
authors.forename: Keiji
authors.initials: K
authors.lastname: Kanemitsu
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008264:Macrophages / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000382:microbiology; D010587:Phagocytosis; D018805:Sepsis / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial