title: Saccharomyces cerevisiae decreases inflammatory responses induced by F4+ enterotoxigenic Escherichia coli in porcine intestinal epithelial cells.
abstract: Probiotic yeasts may provide protection against intestinal inflammation induced by enteric pathogens. In piglets, infection with F4+ enterotoxigenic Escherichia coli (ETEC) leads to inflammation, diarrhea and intestinal damage. In this study, we investigated whether the yeast strains Saccharomyces cerevisiae (Sc, strain CNCM I-3856) and S. cerevisiae variety boulardii (Sb, strain CNCM I-3799) decreased the expression of pro-inflammatory cytokines and chemokines in intestinal epithelial IPI-2I cells cultured with F4+ ETEC. Results showed that viable Sc inhibited the ETEC-induced TNF-α gene expression whereas Sb did not. In contrast, killed Sc failed to inhibit the expression of pro-inflammatory genes. This inhibition was dependent on secreted soluble factors. Sc culture supernatant decreased the TNF-α, IL-1α, IL-6, IL-8, CXCL2 and CCL20 ETEC-induced mRNA. Furthermore, Sc culture supernatant filtrated fraction < 10 kDa displayed the same effects excepted for TNF-α. Thus, our results extended to Sc (strain CNCM I-3856) the inhibitory effects of some probiotic yeast strains onto inflammation.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1282, Infectiologie Animale et Santé Publique, F-37380 Nouzilly (Tours), Indre et Loire, France.
authors.forename: Galliano
authors.initials: G
authors.lastname: Zanello
authors.forename: François
authors.initials: F
authors.lastname: Meurens
authors.forename: Mustapha
authors.initials: M
authors.lastname: Berri
authors.forename: Claire
authors.initials: C
authors.lastname: Chevaleyre
authors.forename: Sandrine
authors.initials: S
authors.lastname: Melo
authors.forename: Eric
authors.initials: E
authors.lastname: Auclair
authors.forename: Henri
authors.initials: H
authors.lastname: Salmon
mesh_terms: D000818:Animals; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D019936:Probiotics / Q000627:therapeutic use*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D013552:Swine / Q000276:immunology / Q000382:microbiology; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control