title: Preparation and hydrolysis of digoxygenin-labeled probes for in situ hybridization of plant tissues.
abstract: INTRODUCTIONAntisense RNA is typically labeled using digoxygenin (DIG), which is subsequently detected with an anti-DIG antibody coupled to alkaline phosphatase. This protocol describes the synthesis and hydrolysis of a DIG-labeled probe to be used for in situ hybridization.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ferrandiz
authors.forename: Allen
authors.initials: A
authors.lastname: Sessions