title: Simple determination of 40 organophosphate pesticides in raw wool using microwave-assisted extraction and GC-FPD analysis.
abstract: A validated analytical method for the multiresidue analysis of 40 organophosphate pesticides (OPs) and conversion products in raw wool has been developed. The method is based on the selective microwave-assisted extraction (MAE) of raw wool with acetonitrile and analysis of extracts by gas chromatography-flame photometric detector. The optimum MAE conditions were 20 min duration at 80 °C with 30 mL of acetonitrile per gram of wool. A validation study was performed according to the European SANCO guidelines 10684/2009. Limits of detection and quantification for all pesticides tested were from 0.01 to 0.2 mg/kg and from 0.2 to 1.0 mg/kg, respectively. The average recoveries of pesticides spiked at different levels were in the range of 70-120% with relative standard deviations of ≤ 20%. The extraction performance was compared to the one obtained with a reference Soxhlet extraction. The method was also applied in the analysis of real wool (after field application) samples.
authors.affiliation: Farmacognosia y Productos Naturales, Facultad de Química, UdelaR , 11800 Montevideo, Uruguay.
authors.forename: Silvina
authors.initials: S
authors.lastname: Niell
authors.forename: Lucia
authors.initials: L
authors.lastname: Pareja
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gonzalez
authors.forename: Joaquin
authors.initials: J
authors.lastname: Gonzalez
authors.forename: Zisis
authors.initials: Z
authors.lastname: Vryzas
authors.forename: M Veronica
authors.initials: MV
authors.lastname: Cesio
authors.forename: Euphemia
authors.initials: E
authors.lastname: Papadopoulou-Mourkidou
authors.forename: Horacio
authors.initials: H
authors.lastname: Heinzen
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D008872:Microwaves; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000302:isolation & purification*; D010575:Pesticides / Q000032:analysis* / Q000302:isolation & purification*; D012756:Sheep; D014935:Wool / Q000737:chemistry*
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides