title: An enzyme-modulated oxygen-producing micro-system for regenerative therapeutics.
abstract: This study suggests the idea of treating oxygen as a drug in a biological environment and demonstrates that it will exhibit a dosage-dependent trend. To accomplish this, a micro-system was fabricated, having hydrogen peroxide as the oxygen-generating source, which was decomposed using catalase, a common enzyme found in nearly all living organisms. The relevance of the proposed micro-system was justified using cell viability assays under well-controlled and fixed conditions. This study was performed under two controlled conditions, normoxia and hypoxia, and tests were carried out using three different configurations of samples under each condition: direct addition of H(2)O(2), H(2)O(2) encapsulated with single layer, and H(2)O(2) encapsulated with double layers. This study demonstrates that the elegantly designed micro-system managed to control the decomposition of H(2)O(2) and avoided direct contact with cells, while also maintaining cell viability under a low oxygen environment.
authors.affiliation: Department of Biomedical Science, Kyungpook National University, Daegu 700-412, Republic of Korea.
authors.forename: Syed Izhar Haider
authors.initials: SI
authors.lastname: Abdi
authors.forename: Sing Muk
authors.initials: SM
authors.lastname: Ng
authors.forename: Jeong Ok
authors.initials: JO
authors.lastname: Lim
mesh_terms: D002374:Catalase / Q000378:metabolism*; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000008:administration & dosage* / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry; D008863:Microspheres; D010100:Oxygen / Q000378:metabolism*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D003692:Delayed-Action Preparations; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen