title: Denaturing protein immunoprecipitation from yeast.
abstract: INTRODUCTIONWhere possible, it is preferable to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background and simplifies protein harvest. Yeast are boiled in an SDS-containing solution to liberate total proteins. This is also much safer than bead-beating radioactive yeast samples.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: William P
authors.initials: WP
authors.lastname: Tansey