title: Effects of anthraquinone extract from Rheum officinale Bail on the physiological responses and HSP70 gene expression of Megalobrama amblycephala under Aeromonas hydrophila infection.
abstract: We evaluated the effect of dietary supplementation with anthraquinone extract (from Rheum officinale Bail) on the resistance to Aeromonas hydrophila infection in Megalobrama amblycephala. The fish were randomly divided into two groups: a control group (fed a standard diet) and a treatment group (standard diet supplemented with 0.1% anthraquinone extract) and fed for 10 weeks. We then challenged the fish with A. hydrophila and recorded mortality and changes in serum cortisol, lysozyme, alkaline phosphatase (ALP), total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and hepatic catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and the relative expression of heat shock protein 70 (HSP70) mRNA for a period of 5 d. Supplementation with 0.1% anthraquinone extract significantly increased serum lysozyme activity before infection, serum ALP activity at 24 h after infection, serum total protein concentration 12 h after infection, hepatic CAT activity 12 h after infection, hepatic SOD activity before infection, and the relative expression of hepatic HSP70 mRNA both before infection and 6 h after infection. In addition, the supplemented group had decreased levels of serum cortisol 6 h after infection, serum AST and ALT activities 12 h after infection, and hepatic MDA content 12 h after infection. Mortality was significantly lower in the treatment group (86.67%) than the control (100%). Our results suggest that ingestion of a basal diet supplemented with 0.1% anthraquinone extract from R. officinale Bail can enhance resistance against pathogenic infections in M. amblycephala.
authors.affiliation: Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081, China. liub@ffrc.cn
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Xianping
authors.initials: X
authors.lastname: Ge
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
authors.forename: Yijin
authors.initials: Y
authors.lastname: He
authors.forename: Yanting
authors.initials: Y
authors.lastname: Cui
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ming
authors.forename: Qunlan
authors.initials: Q
authors.lastname: Zhou
authors.forename: Liangkun
authors.initials: L
authors.lastname: Pan
mesh_terms: D016980:Aeromonas hydrophila; D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005393:Fish Diseases / Q000401:mortality* / Q000517:prevention & control; D005786:Gene Expression Regulation / Q000187:drug effects*; D016905:Gram-Negative Bacterial Infections / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D012250:Rheum / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D018840:HSP70 Heat-Shock Proteins; D010936:Plant Extracts