title: Separation of anthocyanins from Perilla frutescens by high speed countercurrent chromatography.
abstract: OBJECTIVE
  To develop an efficient method for the separation of anthocyanins from Perilla frutescens.
  
  
  METHODS
  Freeze-dried Perilla frutescens was extracted with 1% HCl. After purified by Amberlite XAD-7 column chromatography, the bioactive anthocyanins were separated by high-speed countercurrent chromatography (HSCCC). The structures of the compounds were elucidated by 1H- and 13C-NMR spectroscopy.
  
  
  RESULTS
  When the HSCCC separation was performed with a two-phase solvent system composed of n-butanol-tert-butyl methyl ether-acentonitrile-water (2:2:1:5 + 0.1% TFA) by eluting the mobile phase at a flow rate of 3.0 mL/min and a revolution speed of 800 r/min, 10.1 mg malonylshisonin and 8.6 mg shisonin were obtained from 1.0 g of the XAD-7 extract. The purities of malonylshisonin and shisonin were 96.7% and 97.5%, respectively.
  
  
  CONCLUSIONS
  HSCCC is a fast and efficient technique to prepare pure malonylshisonin and shisonin from Perilla frutescens.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. huxiaodan2008@sina.com.cn
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Hu
authors.forename: Ai-Dong
authors.initials: AD
authors.lastname: Sun
authors.forename: De-Quan
authors.initials: DQ
authors.lastname: Zhang
mesh_terms: D000872:Anthocyanins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003377:Countercurrent Distribution / Q000379:methods*; D031344:Perilla frutescens / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D012997:Solvents