title: Two new phenolic glycosides from the stems of Clematis parviloba.
abstract: To study the chemical constituents of the stems of Clematis parviloba, six compounds were isolated from a 95% ethanol extract by using a combination of various chromatographic techniques including column chromatography over silica gel, ODS, Sephadex LH-20, and semi-preparative HPLC. Two new phenolic glycosides, 2-((E)-3-carboxybut-2-en-yl)-4-hydroxy-3-methyl-phenyl-O-beta-D-glucopyranoside (1) and 4'-hydroxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (2) were isolated, together with a known phenolic glycoside, 4'-hydroxy-3'-methoxy-phenol-beta-D-[6-O-(4"-hydroxy-3", 5"-dimethoxy-benzoate)] glucopyranoside (3) as well as three known megastigmane glycosides, linarionoside A (4), linarionoside C (5), and staphylionoside K (6). Their structures were determined on the basis of spectroscopic analysis and chemical evidence. Among them, compounds 1 and 2 were named as clemaparvilosides A (1) and B (2), respectively, and compounds 3-6 were obtained from Clematis genus for the first time.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Yan
authors.forename: Li-zhen
authors.initials: LZ
authors.lastname: Xu
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Qi-wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: Yang
mesh_terms: D039324:Clematis / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: C575237:2-((E)-3carboxybut-2-en-yl)-4-hydroxy-3-methyl-phenyl-O-beta-D-glucopyranoside; C575238:4'-hydroxy-phenol-beta-D-(6-O-(4''-hydroxy-3'', 5''-dimethoxy-benzoate)); D006027:Glycosides