title: Lubricin immunohistochemical expression in human temporomandibular joint disc with internal derangement.
abstract: Lubricin is a chondroprotective, mucinous glycoprotein which contribute to joint lubrication, especially to boundary lubrication and maintains joint integrity. The present investigation aimed to study the immunolocalization of lubricin in TMJ discs from patients affected by anterior disc displacement with reduction (ADDwR) ADDwoR. Eighteen TMJ displaced disc affected by ADDwoR were processed immunohistochemically, with a polyclonal anti-lubricin antibody, used at 1:50 working dilution. The percentage of lubricin immunopositive cells (extent score = ES) and the extent of lubricin staining of the disc extracellular matrix (ECM), were evaluated. Each sample was scored for histopathological changes. Percentage of immunostained surface disc cells was the same (ES = 4) in both control and ADDwOR cells, being this data not statistically significant (P < 0.05). In pathological specimens the percentages of lubricin-stained cells was very high with an ES of 4 respect to control specimen, and this difference was statistically significant different (P > 0.05). The extracellular matrix (ECM) of discs at the disc surfaces of both pathological and normal specimens was very heavily stained (++++). Both the ES and ECM staining were not statistically correlated to the TMJ degeneration score according to the Spearman's rank correlation coefficient. According to our findings, a longstanding TMJ disc injury, affects lubricin expression in the TMJ disc tissue and not its surfaces, moreover, lubricin immunostaining is not correlated to TMJ disc histopathological changes.
authors.affiliation: Department of Dentistry, Faculty of Dentistry, University of Catania, Policlinico Universitario, Catania, Italy. rleonard@unict.it
authors.forename: Rosalia
authors.initials: R
authors.lastname: Leonardi
authors.forename: Luis Eduardo
authors.initials: LE
authors.lastname: Almeida
authors.forename: Carla
authors.initials: C
authors.lastname: Loreto
mesh_terms: D000328:Adult; D002467:Cell Nucleus / Q000473:pathology; D019902:Chondrocytes / Q000473:pathology; D002863:Chromogenic Compounds; D003094:Collagen; D003593:Cytoplasm / Q000473:pathology; D005109:Extracellular Matrix / Q000737:chemistry; D005110:Extracellular Space / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D004204:Joint Dislocations / Q000473:pathology*; D054327:Lubricants / Q000032:analysis; D008297:Male; D013582:Synovial Fluid / Q000737:chemistry*; D019224:Temporomandibular Joint Disc / Q000473:pathology*; D013705:Temporomandibular Joint Disorders / Q000473:pathology*; D000099158:Proteoglycan 4
chemical_list: D002863:Chromogenic Compounds; D003094:Collagen; D006023:Glycoproteins; D054327:Lubricants; D000099158:Proteoglycan 4