title: [Determination of histatins 5 in salivary by reversed-phase high performance liquid chromatography method with ultraviolet detection].
abstract: OBJECTIVE
  The determination method of histatins 5 in human saliva with reversed-phase high performance liquid chromatography (HPLC) was developed.
  
  
  METHODS
  Salivary samples were collected and diluted with phosphate buffer (pH 2.5). The upper solution was determined with HPLC. Phosphate buffer (pH 3.5) of the mobile phase and C18 column was used throughout the experiment. The detection wavelength was 276 nm.
  
  
  RESULTS
  The linear ranges were 1.0-50.0 microg x mL(-1). The detection limit was 0.12 microg x mL(-1). The relative standard derivations (RSD) of standard solution for reserved time and peak area were 0.68% and 4.13% respectively. The proposed method was applied for analysis of salivary samples and the satisfactory results were obtained. RSD for sample determination was 4.41% and the average recoveries were 88.4%-109.0%.
  
  
  CONCLUSIONS
  The method was quick, simple and accurate. Analytical time was less than 15 min. It was adapted for analysis of salivary histatins 5 in salivary samples.
authors.affiliation: Dept. of Sanitary Technology, West China School of Public Health of Sichuan University, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zeng
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zou
authors.forename: Qing-Fu
authors.initials: QF
authors.lastname: Li
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D054808:Histatins; D006801:Humans; D012463:Saliva
chemical_list: D054808:Histatins