title: Efficacy of ultraviolet radiation as an alternative technology to inactivate microorganisms in grape juices and wines.
abstract: Since sulphur dioxide (SO(2)) is associated with health risks, the wine industry endeavours to reduce SO(2) levels in wines with new innovative techniques. The aim of this study was, therefore, to investigate the efficacy of ultraviolet radiation (UV)-C (254 nm) as an alternative technology to inactivate microorganisms in grape juices and wines. A pilot-scale UV-C technology (SurePure, South Africa) consisting of an UV-C germicidal lamp (100 W output; 30 W UV-C output) was used to apply UV-C dosages ranging from 0 to 3672 J l(-1), at a constant flow rate of 4000 l h(-1) (Re > 7500). Yeasts, lactic and acetic acid bacteria were singly and co-inoculated into 20 l batches of Chenin blanc juice, Shiraz juice, Chardonnay wine and Pinotage wine, respectively. A dosage of 3672 J l(-1), resulted in an average log(10) microbial reduction of 4.97 and 4.89 in Chardonnay and Pinotage, respectively. In Chenin blanc and Shiraz juice, an average log(10) reduction of 4.48 and 4.25 was obtained, respectively. UV-C efficacy may be influenced by liquid properties such as colour and turbidity. These results had clearly indicated significant (p < 0.05) germicidal effect against wine-specific microorganisms; hence, UV-C radiation may stabilize grape juice and wine microbiologically in conjunction with reduced SO(2) levels.
authors.affiliation: Faculty of Applied Sciences, Department of Food Technology, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa.
authors.forename: Ilse N
authors.initials: IN
authors.lastname: Fredericks
authors.forename: Maret
authors.initials: M
authors.lastname: du Toit
authors.forename: Maricel
authors.initials: M
authors.lastname: Krügel
mesh_terms: D001628:Beverages / Q000382:microbiology* / Q000528:radiation effects; D003116:Color; D003258:Consumer Behavior; D003257:Consumer Product Safety; D004307:Dose-Response Relationship, Radiation; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling / Q000379:methods; D005514:Food Irradiation; D006801:Humans; D007777:Lactobacillaceae / Q000254:growth & development / Q000528:radiation effects*; D009391:Nephelometry and Turbidimetry; D014466:Ultraviolet Rays; D027843:Vitis / Q000382:microbiology / Q000528:radiation effects; D014920:Wine / Q000382:microbiology* / Q000528:radiation effects / Q000592:standards; D015003:Yeasts / Q000254:growth & development / Q000528:radiation effects*