title: Differentiation of cfiA-negative and cfiA-positive Bacteroides fragilis isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: Carbapenem resistance in Bacteroides fragilis is associated with cfiA-encoded class B metallo-beta-lactamase. cfiA-negative and cfiA-positive isolates belong to genotypically distinct groups. Of a total of 248 B. fragilis isolates included in this study, 214 were susceptible, 10 were intermediate, and 24 were resistant to meropenem. We show that matrix-assisted laser desorption ionization-time of flight mass spectrometry is able to differentiate between cfiA-negative and cfiA-positive isolates and predict carbapenem resistance in a routine laboratory setting.
authors.affiliation: Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium. Ingrid.wybo@uzbrussel.be
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wybo
authors.forename: Annelies
authors.initials: A
authors.lastname: De Bel
authors.forename: Oriane
authors.initials: O
authors.lastname: Soetens
authors.forename: Fedoua
authors.initials: F
authors.lastname: Echahidi
authors.forename: Kristof
authors.initials: K
authors.lastname: Vandoorslaer
authors.forename: Marina
authors.initials: M
authors.lastname: Van Cauwenbergh
authors.forename: Denis
authors.initials: D
authors.lastname: Piérard
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D001441:Bacteroides fragilis / Q000737:chemistry* / Q000145:classification* / Q000187:drug effects; D006801:Humans; D000077731:Meropenem; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013845:Thienamycins / Q000494:pharmacology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013845:Thienamycins; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem