title: Microarray-based genotyping of Staphylococcus aureus isolates from camels.
abstract: Staphylococcus aureus is a common cause of mastitis and other diseases in camels. In order to obtain data on population structure as well as on the carriage of toxin genes and resistance markers, a collection of 45 isolates from dromedaries of Dubai, United Arab Emirates, were genotyped. These isolates belonged to clonal complexes CC6 (twenty isolates; 44.44%), CC30 (sixteen isolates; 35.56%), CC188 (five isolates; 11.11%), CC152 (1 isolate, 2.2%) and to a previously un-described sequence type (ST1755: arcc-18, aroe-115, glpf-6, gmk-2 pta-109, tpi-50 and yqil-2; three isolates; 6.67%). Resistance genes proved to be rare. Only three out of 45 isolates (6.67%) carried the beta-lactamase operon. The tetracycline resistance gene tetK was also detected in three isolates (6.67%). Neither the mecA gene, defining MRSA, nor other resistance genes were found. Common virulence markers included leukocidin genes lukD+lukE (in twenty-five isolates; 55.56%), the staphylokinase gene sak (twenty-two isolates; 48.89%), the enterotoxin gene cluster egc (fifteen isolates; 33.33%), and a distinct variant of the enterotoxin A gene (sea-320E, GenBank AY196686.1; thirteen isolates; 28.89%). One CC152 isolate was positive for genes encoding the Panton-Valentine leukocidin (lukF-PV+lukS-PV). This study provides first genotyping data on the population structure and the presence of toxin genes and resistance markers of S. aureus strains in Middle Eastern camels.
authors.affiliation: Institute for Medical Microbiology and Hygiene, Faculty of Medicine Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, D-01307 Dresden, Germany. monecke@rocketmail.com
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.forename: Peter
authors.initials: P
authors.lastname: Slickers
authors.forename: Renate
authors.initials: R
authors.lastname: Wernery
authors.forename: Bobby
authors.initials: B
authors.lastname: Johnson
authors.forename: Sherry
authors.initials: S
authors.lastname: Jose
authors.forename: Ulrich
authors.initials: U
authors.lastname: Wernery
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002162:Camelus; D005838:Genotype; D008892:Milk / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D014479:United Arab Emirates