title: Immunogenicity and physico-chemical characterisation of a candidate conjugate vaccine against group B streptococcus serotypes Ia, Ib and III.
abstract: The immunogenicity and physico-chemical characteristics of a candidate conjugate vaccine against group B streptococcus serotypes Ia, Ib and III, were evaluated. The level and functional activity of serotype-specific antibody responses induced by monovalent and combined formulations were investigated using a mouse model and in vitro opsonophagocytosis assay. Molecular sizing of the conjugates and integrity of the intermediate components were evaluated by optical spectroscopy and size exclusion chromatography. All three serotypes induced substantial antibody responses which were functionally active. Combining the three serotypes did not seem to affect the antibody responses to individual serotypes, except when given at high dose, where the IgG response to serotype III but not the opsonophagocytic activity was slightly reduced compared to monovalent administration.
authors.affiliation: Bacteriology Division, National Institute for Biological Standards and Control, Health Protection Agency, Potters Bar, Hertfordshire EN6 3QG, UK.
authors.forename: Lorna
authors.initials: L
authors.lastname: Lancaster
authors.forename: Manolya
authors.initials: M
authors.lastname: Saydam
authors.forename: Kevin
authors.initials: K
authors.lastname: Markey
authors.forename: Mei Mei
authors.initials: MM
authors.lastname: Ho
authors.forename: Fatme
authors.initials: F
authors.lastname: Mawas
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D015373:Bacterial Typing Techniques; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins / Q000097:blood; D010587:Phagocytosis; D012703:Serotyping; D013290:Streptococcal Infections / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D009895:Opsonin Proteins; D022541:Streptococcal Vaccines; D018074:Vaccines, Conjugate