title: Reproductive toxic potential of panmasala in male Swiss albino mice.
abstract: Some ingredients of panmasala have the ability to penetrate the blood-testis barrier but the reproductive toxic potential of panmasala has not been studied. This study is aimed to assess the possible damage caused by panmasala to male reproductive system in mice. Swiss albino male mice were randomly divided into 7 groups receiving either standard control diet or panmasala-containing diet. Three doses (0.5%, 1.5% and 3%) of panmasala plain (PMP) as well as panmasala with tobacco (PMT)-gutkha were given for a period of 6 months. Assessment of organ weight, sperm count and morphology, spermatid count, sperm production, testicular 17β-hydroxysteroid dehydrogenase (17β-HSD) activity and histology were conducted. A nonsignificant decrease in absolute and relative weight of testis and epididymis was observed. Spermatid count, sperm count and production were significantly decreased and 17β-HSD activity was found considerably declined at 3% of both PMP- and PMT-treated groups as compared to control. The histological observations revealed panmasala induced testicular damage. Abnormal morphology of sperm head shape was significantly elevated in higher doses of both types of panmasala-treated groups than control. The results suggests that panmasala has reproductive toxic potential and more alteration is seen with gutkha as compared to panmasala plain, indicating that similar effects might also be possible in humans.
authors.affiliation: Division of Reproductive and Cytotoxicology, National Institute of Occupational Health, Ahmedabad, India.
authors.forename: A
authors.initials: A
authors.lastname: Kumari
authors.forename: Bn
authors.initials: B
authors.lastname: Mojidra
authors.forename: Ak
authors.initials: A
authors.lastname: Gautam
authors.forename: Y
authors.initials: Y
authors.lastname: Verma
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001114:Areca / Q000633:toxicity*; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D028321:Plant Preparations / Q000008:administration & dosage / Q000633:toxicity*; D013076:Sperm Count; D013077:Sperm Head / Q000187:drug effects; D013087:Spermatids / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D014030:Tobacco, Smokeless / Q000633:toxicity*; D036401:Toxicity Tests, Chronic
chemical_list: D028321:Plant Preparations; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase