title: Surveillance of methicillin-resistant Staphylococcus aureus isolated in Torino (northwest Italy).
abstract: Nosocomial infections by methicillin-resistant Staphylococcus aureus (MRSA) are an increasing cause of morbidity and mortality. Recently, a worldwide increase of community-acquired MRSA infections has also been recorded. The purpose of this study was to assess the frequency of MRSA isolation from in- and outpatients admitted to an academic teaching hospital near Torino (northwest Italy) in 1 year and to characterize 90 clinical isolates of MRSA collected in the same period. Antimicrobial susceptibility and the presence in the isolates of the Panton-Valentine leukocidin (PVL) gene were assessed. Molecular epidemiology was performed by SCCmec and capsule typing, and by pulsed-field gel electrophoresis. The global proportion of MRSA isolated was 33.1%. Characterization performed on 90 MRSA revealed a high percentage of resistance to ciprofloxacin and erythromycin, and the presence of the PVL gene in one strain only. Most of the MRSA strains circulating in the Torino district belonged to SCCmec types II and I, and the 67.6% resulted positive for the cap 5 gene. The pulsotype analysis permitted to observe a clonal heterogeneity of the isolates and a higher similarity in relation to singular mec types; only few nosocomial clones could account for a local outbreak of a sporadic isolate.
authors.affiliation: Department of Clinical and Biological Sciences, University of Torino, S. Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano (Turin), Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Crivellaro
authors.forename: Ilaria
authors.initials: I
authors.lastname: Leone
authors.forename: Ornella
authors.initials: O
authors.lastname: Bianco
authors.forename: Dianella
authors.initials: D
authors.lastname: Savoia
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002939:Ciprofloxacin / Q000494:pharmacology; D017714:Community-Acquired Infections / Q000453:epidemiology* / Q000382:microbiology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D005098:Exotoxins / Q000235:genetics; D006784:Hospitals, Teaching; D006801:Humans; D020071:Interspersed Repetitive Sequences; D007558:Italy / Q000453:epidemiology; D007956:Leukocidins / Q000235:genetics; D016106:Methicillin Resistance / Q000235:genetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D018571:Sentinel Surveillance; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013204:Staphylococcus Phages / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D002939:Ciprofloxacin; D004917:Erythromycin