title: Effect of in-vitro addition of sulfated xylans, glucosans or chondroitins on the prothrombin time of human plasma and on the enhancement of activation of glutamic plasminogen by tissue plasminogen activator.
abstract: Three each of sulfated xylans, glucosans or chondroitins were investigated. A comparison of the infrared analysis of the native and sulfated amylopectin showed the presence of peaks corresponding to 800/cm for S-O and 1200-1300/cm for S=O only in the sulfated amylopectin and earlier results using C NMR showed sulfation of hyroxymethyl groups (C-6) of amylopectin. Based upon % sulfate the three classes of compounds following sulfation contained more than one sulfate group per sugar unit. All of them exhibited significant in-vitro anticoagulant property by inhibiting thrombin generation at very low concentrations. In general sulfated xylans or glucosans were better anticoagulants than the chondroitins. The results of in-vitro studies of the activation of glutamic plasminogen (Glu-Plg) by tissue plasminogen activator (t-PA) using 0.05 mol/l Tris buffer (pH 7.35) containing physiological concentration of NaCl (0.9%), showed that oat spelts xylan sulfate and amylopectin sulfate gave a 20-fold enhancement of the activation in a synergistic manner when used in combination with 32.4 mmol/l of lysine. The mechanism of enhancement was investigated by dilution studies. Sulfated amylopectin interacted with Glu-Plg but not with t-PA and lysine which interacted with both Glu-Plg and t-PA enhanced the activation in a synergistic manner using low concentrations of t-PA.
authors.affiliation: Department of Chemistry, Prairie View A&M University, Prairie View, Texas 77445, USA.
authors.forename: Erinn
authors.initials: E
authors.lastname: McMillan
authors.forename: Mabunatu
authors.initials: M
authors.lastname: Roberts
authors.forename: Vasant M
authors.initials: VM
authors.lastname: Doctor
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000494:pharmacology*; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010426:Pentosan Sulfuric Polyester / Q000737:chemistry / Q000494:pharmacology*; D010958:Plasminogen / Q000378:metabolism; D011517:Prothrombin Time; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000378:metabolism
chemical_list: D000925:Anticoagulants; D005936:Glucans; D013431:Sulfates; D010426:Pentosan Sulfuric Polyester; D010958:Plasminogen; D002809:Chondroitin Sulfates; D010959:Tissue Plasminogen Activator