title: Suspended moxibustion relieves chronic visceral hyperalgesia and decreases hypothalamic corticotropin-releasing hormone levels.
abstract: OBJECTIVE
  To evaluate the effect of suspended moxibustion (SM) on rectal sensory thresholds and to analyze the possible mechanisms involved in SM treatment of chronic visceral hypersensitivity (CVH) in rats.
  
  
  METHODS
  SM was administered once daily to 37-d-old CVH rats for 7 d. The two pairs of acupoints (ST25 and ST37, bilateral) were simultaneously treated with SM. Each treatment lasted for 30 min. Rats undergoing treatment with SM were not anesthetized. Untreated CVH rats and normal rats were used as controls. The abdominal withdrawal reflex was determined 30-90 min after the seven treatments. The hypothalamic corticotropin-releasing hormone (CRH) mRNA level was measured using real-time quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  We found that SM treatment significantly decreased visceral sensitivity to colorectal distention in this rat model. In treated animals, SM also decreased the relative hypothalamic CRH mRNA expression level to control levels.
  
  
  CONCLUSIONS
  Lower hypothalamic CRH levels may mediate the beneficial effects of SM in this rat irritable bowel syndrome model.
authors.affiliation: Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China.
authors.forename: En-Hua
authors.initials: EH
authors.lastname: Zhou
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Ding
authors.forename: Huan-Gan
authors.initials: HG
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Liu
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D004108:Dilatation, Pathologic; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000378:metabolism / Q000503:physiopathology* / Q000628:therapy*; D007031:Hypothalamus / Q000378:metabolism*; D043183:Irritable Bowel Syndrome / Q000503:physiopathology; D008297:Male; D008516:Medicine, Chinese Traditional; D009071:Moxibustion / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012020:Reflex, Abdominal; D014781:Viscera / Q000503:physiopathology*
chemical_list: D003346:Corticotropin-Releasing Hormone