title: Enhanced radiosensitivity in 1,25-dihydroxyvitamin D3 deficient mice.
abstract: To investigate whether impaired osteogenesis resulting from vitamin D deficiency can influence hematopoiesis recovery after radiation, the 25-hydroxyvitamin D-1α-hydroxylase (1α-hydroxylase) gene knockout (KO) mice and wild type (WT) mice were subjected to different doses of gamma ray. The survival rates, peripheral blood cell counts and bone marrow cellularity were studied after irradiation (IR). The survival rates of the KO mice were significantly lower than that of WT mice after 6 or 8 Gy dose of radiation. The recovery of white blood cells in KO mice was significantly delayed compared with that in WT mice after radiation. The red blood cell number in WT mice was observed to increase more than that in KO mice at days 14 and 28 after radiation. The nadir platelet count in KO mice was nearly half of that in WT mice. Dramatically higher bone marrow cell numbers were found in WT mice compared with KO mice. Our findings demonstrate the enhanced radiosensitivity in 1,25-dihydroxyvitamin D3 (1,25-(OH)(2)D(3)) deficient mice.
authors.affiliation: Department of Toxicology, School of Radiation Medicine and Public Health, Soochow University, Suzhou, Jiangsu, 215123, The People’s Republic of China.
authors.forename: Zeng-Li
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Ding
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
authors.forename: Bing-Yan
authors.initials: BY
authors.lastname: Li
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000235:genetics*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002117:Calcitriol / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006579:Heterozygote; D007962:Leukocytes / Q000166:cytology; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D010976:Platelet Count; D011836:Radiation Tolerance; D013997:Time Factors
chemical_list: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D002117:Calcitriol