title: A murine model of xenotransplantation of human glioblastoma with immunosuppression by orogastric cyclosporin.
abstract: Several animal experimental models have been used in the study of malignant gliomas. The objective of the study was to test the efficacy of a simple, reproducible and low cost animal model, using human cells of glioblastoma multiforme (GBM) xenotransplantated in subcutaneous tissue of Wistar rats, immunosuppressed with cyclosporin given by orogastric administration, controlled by nonimunosuppressed rats. The animals were sacrificed at weekly intervals and we have observed gradual growth of tumor in the immunosuppressed group. The average tumor volume throughout the experiment was 4.38 cm(3) in the immunosuppressed group, and 0.27 cm(3) in the control one (p<0.001). Tumors showed histopathological hallmarks of GBM and retained its glial identity verified by GFAP and vimentin immunoreaction. Immunosuppression of rats with cyclosporin was efficient in allowing the development of human glioblastoma cells in subcutaneous tissues. The model has demonstrated the maintenance of most of the histopathological characteristics of human glioblastoma in an heterotopic site and might by considered in research of molecular and proliferative pathways of malignant gliomas.
authors.affiliation: Division of Neurosurgery, Department of Surgery, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, RJ, Brazil. a.m.cunha@uol.com.br
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Cunha
authors.forename: Fernanda S
authors.initials: FS
authors.lastname: Nascimento
authors.forename: Jane C O F
authors.initials: JC
authors.lastname: Amaral
authors.forename: Sandra
authors.initials: S
authors.lastname: Konig
authors.forename: Crhistina M
authors.initials: CM
authors.lastname: Takiya
authors.forename: Vivaldo
authors.initials: V
authors.lastname: M Neto
authors.forename: Eduardo
authors.initials: E
authors.lastname: Rocha
authors.forename: Jorge P B M
authors.initials: JP
authors.lastname: Souza
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001932:Brain Neoplasms / Q000737:chemistry / Q000473:pathology*; D016572:Cyclosporine / Q000008:administration & dosage*; D005909:Glioblastoma / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D008297:Male; D023421:Models, Animal; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous / Q000379:methods; D047368:Tumor Burden; D014746:Vimentin / Q000032:analysis
chemical_list: D007166:Immunosuppressive Agents; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D014746:Vimentin; D016572:Cyclosporine