title: Electrical characterization of DNA-functionalized solid state nanopores for bio-sensing.
abstract: We present data concerning the electrical properties of a class of biosensor devices based on bio-functionalized solid state nanopores able to detect different kinds of interactions between probe molecules, chemically attached to the pore surface, and target molecules present in solution and electrophoretically drawn through the nanometric channel. The great potentiality of this approach resides in the fact that the functionalization of a quite large pore (up to 50-60 nm) allows a sufficient diameter reduction for the attainment of a single molecule sensing dimension and selective activation, without the need for further material deposition, such as metal or oxides, or localized surface modification. The results indicate that it will be possible, in the near future, to conceive and design devices for parallel analysis of biological samples made of arrays of nanopores differently functionalized, fabricated by standard lithographic techniques, with important applications in the field of molecular diagnosis.
authors.affiliation: Nanomed Labs, Physics Department, University of Genova, and Nanobiotechnologies, National Institute of Cancer Research (IST), Largo R Benzi, 10 Genova, 16132, Italy. mussi@fisica.unige.it
authors.forename: V
authors.initials: V
authors.lastname: Mussi
authors.forename: P
authors.initials: P
authors.lastname: Fanzio
authors.forename: L
authors.initials: L
authors.lastname: Repetto
authors.forename: G
authors.initials: G
authors.lastname: Firpo
authors.forename: P
authors.initials: P
authors.lastname: Scaruffi
authors.forename: S
authors.initials: S
authors.lastname: Stigliani
authors.forename: M
authors.initials: M
authors.lastname: Menotta
authors.forename: M
authors.initials: M
authors.lastname: Magnani
authors.forename: G P
authors.initials: GP
authors.lastname: Tonini
authors.forename: U
authors.initials: U
authors.lastname: Valbusa
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity
chemical_list: D004247:DNA