title: Inhibitory effects of Zuo-Jin-Wan and its alkaloidal ingredients on activator protein 1, nuclear factor-κB, and cellular transformation in HepG2 cells.
abstract: Zuo-Jin-Wan (ZJW) has been used to treat hepatocellular carcinoma in Asia. This study was to determine whether ZJW and its components blocked activator protein 1 (AP-1) and nuclear factor-κB (NF-κB) activities as well as tumor promotion in hepatoblastoma HepG2 cells. ZJW and its components, Coptis chinensis and Evodia rutaecarpa, inhibited AP-1 and NF-κB activities, and suppressed anchorage-independent growth of HepG2 cells. The major alkaloidal ingredients, berberine and evodiamine, inhibited AP-1 activities and/or NF-κB activation, and further suppressed hepatocellular transformation. In conclusion, ZJW and its constituents, berberine and evodiamine, suppressed tumor promotion primarily through AP-1 and/or NF-κB pathways in HepG2 cells.
authors.affiliation: Graduate Institute of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: De-Cheng
authors.initials: DC
authors.lastname: Chao
authors.forename: Li-Jen
authors.initials: LJ
authors.lastname: Lin
authors.forename: Shung-Te
authors.initials: ST
authors.lastname: Kao
authors.forename: Hui-Chi
authors.initials: HC
authors.lastname: Huang
authors.forename: Chih-Shiang
authors.initials: CS
authors.lastname: Chang
authors.forename: Ji-An
authors.initials: JA
authors.lastname: Liang
authors.forename: Shih-Lu
authors.initials: SL
authors.lastname: Wu
authors.forename: Chien-Yun
authors.initials: CY
authors.lastname: Hsiang
authors.forename: Tin-Yun
authors.initials: TY
authors.lastname: Ho
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001599:Berberine / Q000302:isolation & purification / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000187:drug effects*; D039323:Coptis / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D032086:Evodia / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy; D016328:NF-kappa B / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D011799:Quinazolines / Q000302:isolation & purification / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate; D018808:Transcription Factor AP-1 / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D010936:Plant Extracts; D011799:Quinazolines; D018808:Transcription Factor AP-1; C560510:Zuo-Jin-Wan; D001599:Berberine; C049639:evodiamine; D013755:Tetradecanoylphorbol Acetate