title: [Determination of quercetin and kaempferol in Dysosma plants by RP-HPLC].
abstract: OBJECTIVE
  To determine quercetin and kaempferol in the plant of genus Dysosma that come from different species, different plant parts or different growing areas, which provide the basis of rational utilization of Dysosma plants.
  
  
  METHODS
  The analysis was performed on a Diamonsil C18 column (4.6 mm x 150 mm, 5 microm) eluted with the mobile phase of methanol-water containing 0.1% phosphoric acid (60:40). The flow rate was 1 mL x min(-1), the detection wavelength was 360 nm; and the column temperature was set at 25 degrees C.
  
  
  RESULTS
  The linear ranges of quercetin and kaempferol are 0.22-1.1 microg and 0.42-2.1 microg. The average recoveries of quercetin and kaempferol are 97.1% (RSD 1.4%) and 99.6% (RSD 2.4%); respectively.
  
  
  CONCLUSIONS
  The contents of flavones in different species of Dysosma are significantly different.
authors.affiliation: First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Wan
authors.forename: Shunzhi
authors.initials: S
authors.lastname: He
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D028141:Berberidaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D044949:Kaempferols / Q000032:analysis*; D011794:Quercetin / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D044949:Kaempferols; C006552:kaempferol; D011794:Quercetin