title: Cancer stem cell-like cells from a single cell of oral squamous carcinoma cell lines.
abstract: Resistance of oral squamous cell carcinomas (OSCC) to conventional chemotherapy or radiation therapy might be due to cancer stem cells (CSCs). The development of novel anticancer drugs requires a simple method for the enrichment of CSCs. CSCs can be enriched from OSCC cell lines, for example, after cultivation in serum-free cell culture medium (SFM). In our study, we analyzed four OSCC cell lines for the presence of CSCs. CSC-like cells could not be enriched with SFM. However, cell lines obtained from holoclone colonies showed CSC-like properties such as a reduced rate of cell proliferation and a reduced sensitivity to Paclitaxel in comparison to cells from the parental lineage. Moreover, these cell lines differentially expressed the CSC-marker CD133, which is also upregulated in OSCC tissues. Interestingly, CD133(+) cells in OSCC tissues expressed little to no Ki67, the cell proliferation marker that also indicates reduced drug sensitivity. Our study shows a method for the isolation of CSC-like cell lines from OSCC cell lines. These CSC-like cell lines could be new targets for the development of anticancer drugs under in vitro conditions.
authors.affiliation: Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Felthaus
authors.forename: T
authors.initials: T
authors.lastname: Ettl
authors.forename: M
authors.initials: M
authors.lastname: Gosau
authors.forename: O
authors.initials: O
authors.lastname: Driemel
authors.forename: G
authors.initials: G
authors.lastname: Brockhoff
authors.forename: A
authors.initials: A
authors.lastname: Reck
authors.forename: K
authors.initials: K
authors.lastname: Zeitler
authors.forename: M
authors.initials: M
authors.lastname: Hautmann
authors.forename: T E
authors.initials: TE
authors.lastname: Reichert
authors.forename: G
authors.initials: G
authors.lastname: Schmalz
authors.forename: C
authors.initials: C
authors.lastname: Morsczeck
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015195:Drug Design; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D051153:Wnt Proteins; D017239:Paclitaxel