title: Association between mitochondrial DNA haplotype compatibility and increased efficiency of bovine intersubspecies cloning.
abstract: Reconstructed embryos derived from intersubspecies somatic cell nuclear transfer (SCNT) have poorer developmental potential than those from intrasubspecies SCNT. Based on our previous study that Holstein dairy bovine (HD) mitochondrial DNA (mtDNA) haplotype compatibility between donor karyoplast and recipient cytoplast is crucial for SCNT embryo development, we performed intersubspecies SCNT using HD as donor karyoplast and Luxi yellow heifer (LY) as recipient cytoplast according to mtDNA haplotypes determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The results demonstrated that intersubspecies mtDNA homotype SCNT embryos had higher pre- and post-implantation developmental competence than intrasubspecies mtDNA heterotype embryos as well as improved blastocyst reprogramming status, including normal H3K9 dimethylation pattern and promoter hypomethylation of pluripotent genes such as Oct4 and Sox2, suggesting that intersubspecies SCNT using LY oocytes maintains HD cloning efficiency and may reprogram HD nuclei to develop into a normal cloned animal ultimately. Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HD cloning.
authors.affiliation: Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, 24/1400 West Beijing Road, Shanghai 200040, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Yan
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Ma
authors.forename: Fei
authors.initials: F
authors.lastname: Jiao
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Huang
authors.forename: Fanyi
authors.initials: F
authors.lastname: Zeng
authors.forename: Yitao
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle / Q000196:embryology / Q000235:genetics*; D019976:Cloning, Organism; D004272:DNA, Mitochondrial / Q000235:genetics*; D003612:Dairying; D010064:Embryo Implantation / Q000235:genetics; D005260:Female; D006239:Haplotypes / Q000235:genetics*; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D053652:Nuclear Transfer Techniques; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D006657:Histones; D000255:Adenosine Triphosphate; D008239:Lysine