title: Inoculation and passaging of Mammalian cell suspension cultures.
abstract: INTRODUCTIONThis protocol describes the growth and passaging of mammalian cells in suspension culture using a simple dilution procedure. In situations in which total replacement of medium is desired, the cells can be gently pelleted by centrifugation and resuspended in the appropriate medium.
authors.forename: Gretchen J
authors.initials: GJ
authors.lastname: Darlington