title: The role of dynamin in HIV type 1 Env-mediated cell-cell fusion.
abstract: HIV-1 envelope glycoproteins are the key viral proteins that mediate HIV-1 entry and cell-cell fusion. In contrast to HIV-1 entry, the mechanism of HIV-1 Env-mediated cell-cell fusion is relatively unclear. This study demonstrated that dynasore, a dynamin inhibitor, suppressed HIV-1 Env-mediated cell-cell fusion. Dynasore sensitivity of HIV-1 Env-mediated cell-cell fusion varied depending on the viral strains. Results from testing a panel of gp41 cytoplasmic tail truncation mutants suggested that the gp41 cytoplasmic tail might play a role in dynasore sensitivity. HIV-1 Env-mediated cell-cell fusion could also be suppressed by a dynamin dominant-negative mutant DNM2(K44A). In summary, these results suggested that dynamin 2 might play a role in HIV-1 Env-mediated cell-cell fusion.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Weihong
authors.initials: W
authors.lastname: Lai
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Phong
authors.initials: P
authors.lastname: Ho
authors.forename: David
authors.initials: D
authors.lastname: Montefiori
authors.forename: Chin-Ho
authors.initials: CH
authors.lastname: Chen
mesh_terms: D002459:Cell Fusion; D034285:Dynamin II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015497:HIV-1 / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006835:Hydrazones / Q000378:metabolism; D054299:env Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
chemical_list: D006835:Hydrazones; C511794:N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide; D054299:env Gene Products, Human Immunodeficiency Virus; D034285:Dynamin II