title: [Photochemical damages to suspended hepatoma carcinoma cells by a new photosensitizer (YWW007)].
abstract: OBJECTIVE
  To investigate the inactivate effect of YWW007 mediated PCT on suspended hepatoma carcinoma cells.
  
  
  METHODS
  The cultured hepatoma carcinoma cells were prepared as 10(9)/L of single cell suspension. Then the cells were subject to dark reaction and PCT in turn with different concentrations of YWW007. The cell mortality was detected by trypan blue exclusion and FCM.
  
  
  RESULTS
  Large amount of necrotic and apoptotic cells were found. The mortality of the cells increased with the increase of the concentration of YWW007. The mortality of the cells reached 99% when the concentration of YWW007 was over 8 micromol/L. Dark cytotoxicity occurred in the cells without exposure to PCT, but at a lower level compared with those exposed to PCT.
  
  
  CONCLUSIONS
  YWW007 mediated PCT can inactivate the suspended hepatoma carcinoma cells, which appear predominately as necrosis. The optimum concentrations of YWW007 are 8 micromol/L-16 micromol/L.
authors.affiliation: Department of Anesthesia, West China Hospital, Sichuan Univercity, Chengdu 610041, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
authors.forename: Chun-hui
authors.initials: CH
authors.lastname: Yang
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Jin-xing
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D010777:Photochemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents