title: PNA containing isocytidine nucleobase: synthesis and recognition of double helical RNA.
abstract: Peptide nucleic acid (PNA1) containing a 5-methylisocytidine (iC) nucleobase has been synthesized. Triple helix formation between PNA1 and RNA hairpins having variable base pairs interacting with iC was studied using isothermal titration calorimetry. The iC nucleobase recognized the proposed target, C-G inversion in polypurine tract of RNA, with slightly higher affinity than the natural nucleobases, though the sequence selectivity of recognition was low. Compared to non-modified PNA, PNA1 had lower affinity for its RNA target.
authors.affiliation: Department of Chemistry, Binghamton University, The State University of New York, Binghamton, NY 13902, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Zengeya
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Eriks
authors.initials: E
authors.lastname: Rozners
mesh_terms: D003562:Cytidine / Q000737:chemistry*; D020135:Peptide Nucleic Acids; D012330:RNA, Double-Stranded / Q000737:chemistry*
chemical_list: D020135:Peptide Nucleic Acids; D012330:RNA, Double-Stranded; C083576:isocytidine; D003562:Cytidine