title: [Effect of TSP-2 antibody against a single epitope of mouse Toll-like receptor 2 extracellular domain on nuclear factor-kappa B and cytokine expression in the intestine of septic mice].
abstract: OBJECTIVE
  To observe the effect of the antibody TSP-2 against a single epitope of mouse Toll-like receptor 2 extracellular domain (mTLR2ECD) on the expression of nuclear factor-kappa B (NF-κB) and cytokines in the intestinal tissue of septic mice.
  
  
  METHODS
  Male BALB/c mice were randomly divided into 4 groups, namely the sham-operated group, model group, TSP-2 treatment group and rabbit IgG treatment group. Sepsis was induced by cecal ligation and puncture (CLP), and at 6, 12 or 24 h after the operation, the ileal tissues were harvested from the mice for HE staining. NF-κB expression was detected with immunohistochemistry. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA expressions were detected with qRT-PCR and their protein expressions by ELISA.
  
  
  RESULTS
  The NF-κB expression in the intestinal tissue significantly increased in the model group as compared with that in the sham- operated group, and decreased after TSP-2 treatment. The model group also showed significantly increased expression levels of TNF-α and IL-6 mRNA and protein in the intestinal tissue (P<0.05), which were lowered by TSP-2 (P<0.05) but not by rabbit IgG treatment (P>0.05).
  
  
  CONCLUSIONS
  The TSP-2 antibody can protect the intestine and delay the development of sepsis by inhibiting NF-κB activation and down-regulating TNF-α and IL-6 expressions in mice.
authors.affiliation: Department of Cell Biology, Southern Medical University, Guangzhou 510515, China. enpinghuang@163.com
authors.forename: En-ping
authors.initials: EP
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Cui-lan
authors.initials: CL
authors.lastname: Yang
authors.forename: Shen-qiu
authors.initials: SQ
authors.lastname: Luo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D016056:Immunodominant Epitopes / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D011897:Random Allocation; D011956:Receptors, Cell Surface / Q000276:immunology; D018805:Sepsis / Q000378:metabolism*; D019699:Thrombospondins / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D000099262:Thrombospondin 2
chemical_list: D000906:Antibodies; D016056:Immunodominant Epitopes; D015850:Interleukin-6; D016328:NF-kappa B; D011956:Receptors, Cell Surface; D019699:Thrombospondins; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha; D000099262:Thrombospondin 2; C493486:Tlr2 protein, mouse; C052131:extracellular matrix receptor