title: In situ synthesis of palladium nanoparticle-graphene nanohybrids and their application in nonenzymatic glucose biosensors.
abstract: A nonenzymatic electrochemical biosensor was developed for the detection of glucose based on an electrode modified with palladium nanoparticles (PdNPs)-functioned graphene (nafion-graphene). The palladium nanoparticle-graphene nanohybrids were synthesized using an in situ reduction process. Nafion-graphene was first assembled onto an electrode to chemically adsorb Pd(2+). And Pd(2+) was subsequently reduced by hydrazine hydrate to form PdNPs in situ. Such a PdNPs-graphene nanohybrids-based electrode shows a very high electrochemical activity for electrocatalytic oxidation of glucose in alkaline medium. The proposed biosensor can be applied to the quantification of glucose with a wide linear range covering from 10 μM to 5mM (R=0.998) with a low detection limit of 1 μM. The experiment results also showed that the sensor exhibits good reproducibility and long-term stability, as well as high selectivity with no interference from other potential competing species.
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Lu
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Li
authors.forename: Fengli
authors.initials: F
authors.lastname: Qu
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhang
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Shen
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry; D010084:Oxidation-Reduction; D010165:Palladium / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D010165:Palladium; D006108:Graphite; D005947:Glucose