title: Protein O-GlcNAcylation regulates Drosophila growth through the insulin signaling pathway.
abstract: Modification of nuclear and cytosolic proteins by O-linked N-acetylglucosamine (O-GlcNAcylation) is ubiquitous in cells. The in vivo function of the protein O-GlcNAcylation, however, is not well understood. Here, we manipulated the cellular O-GlcNAcylation level in Drosophila and found that it promotes developmental growth by enhancing insulin signaling. This increase in growth is due mainly to cell growth and not to cell proliferation. Our data suggest that the increase in the insulin signaling activity is mediated, at least in part, through O-GlcNAcylation of Akt. These results indicate that O-GlcNAcylation is one of the crucial mechanisms involved in control of insulin signaling during Drosophila development.
authors.affiliation: Department of Integrated OMICS for Biomedical Science, WCU Program of Graduate School, Yonsei University, Seoul 120-749, Korea.
authors.forename: Sujin
authors.initials: S
authors.lastname: Park
authors.forename: Si-Hyoung
authors.initials: SH
authors.lastname: Park
authors.forename: Ju Yuel
authors.initials: JY
authors.lastname: Baek
authors.forename: Ye Jin
authors.initials: YJ
authors.lastname: Jy
authors.forename: Kwan Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Jürgen
authors.initials: J
authors.lastname: Roth
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Cho
authors.forename: Kwang-Min
authors.initials: KM
authors.lastname: Choe
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001823:Body Composition; D049109:Cell Proliferation; D004330:Drosophila / Q000254:growth & development* / Q000378:metabolism*; D006031:Glycosylation; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D007328:Insulin / Q000378:metabolism*; D017351:N-Acetylglucosaminyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction
chemical_list: D007328:Insulin; D034741:RNA, Small Interfering; D017351:N-Acetylglucosaminyltransferases; D051057:Proto-Oncogene Proteins c-akt