title: Genetic analysis of ORF5 in porcine reproductive and respiratory syndrome virus in Japan.
abstract: In recent years, no reports regarding genetic information on porcine reproductive and respiratory syndrome virus (PRRSV) with a focus on Japan have been published. To clarify the effect of time on PRRSV genomic evolution, we sequenced the open reading frame 5 (600 or 603 bases) obtained from Japanese PRRSV isolates for three periods (1992-1993, 2000-2001, and 2007-2008) and compared their phylogenetic relationships. Assessment of mean pairwise homology of nucleotide sequences of PRRSV isolates indicated a trend towards increasing heterogeneity over time. In addition, we newly detected a virus classified in cluster IV, indicative of the increasing genetic variation of PRRSV in Japan.
authors.affiliation: Viral Diseases Research Team, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iseki
authors.forename: Michihiro
authors.initials: M
authors.lastname: Takagi
authors.forename: Ayako
authors.initials: A
authors.lastname: Miyazaki
authors.forename: Ken
authors.initials: K
authors.lastname: Katsuda
authors.forename: Osamu
authors.initials: O
authors.lastname: Mikami
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
mesh_terms: D000818:Animals; D001483:Base Sequence; D019143:Evolution, Molecular; D014644:Genetic Variation; D007564:Japan; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D010802:Phylogeny; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D017385:Sequence Homology; D013552:Swine; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D014764:Viral Proteins