title: Antimicrobial actions of α-mangostin against oral streptococci.
abstract: The increasing prevalence of dental caries is making it more of a major world health problem. Caries is the direct result of acid production by cariogenic oral bacteria, especially Streptococcus mutans. New and better antimicrobial agents active against cariogenic bacteria are badly needed, especially natural agents derived directly from plants. We have evaluated the inhibitory actions of α-mangostin, a xanthone purified from ethanolic extracts of the tropical plant Garcinia mangostana L., by repeated silica gel chromatography. α-Mangostin was found to be a potent inhibitor of acid production by S. mutans UA159, active against membrane enzymes, including the F(H+)-ATPase and the phosphoenolpyruvate - sugar phosphotransferase system. α-Mangostin also inhibited the glycolytic enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, and lactic dehydrogenase. Glycolysis by intact cells in suspensions or biofilms was inhibited by α-mangostin at concentrations of 12 and 120 µmol·L⁻¹, respectively, in a pH-dependent manner, with greater potency at lower pH values. Other targets for inhibition by α-mangostin included (i) malolactic fermentation, involved in alkali production from malate, and (ii) NADH oxidase, the major respiratory enzyme for S. mutans. The overall conclusion is that α-mangostin is a multitarget inhibitor of mutans streptococci and may be useful as an anticaries agent.
authors.affiliation: Institute of Biotechnology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam.
authors.forename: Phuong T M
authors.initials: PT
authors.lastname: Nguyen
authors.forename: Robert E
authors.initials: RE
authors.lastname: Marquis
mesh_terms: D000468:Alkalies / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D005285:Fermentation; D029762:Garcinia mangostana / Q000737:chemistry; D006019:Glycolysis; D008293:Malates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D013295:Streptococcus mutans / Q000187:drug effects* / Q000201:enzymology; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D000468:Alkalies; D000900:Anti-Bacterial Agents; D008293:Malates; D010936:Plant Extracts; D047428:Protein Kinase Inhibitors; D044004:Xanthones; C030298:malic acid; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D006180:Proton-Translocating ATPases; D010100:Oxygen; C021053:mangostin