title: Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.
abstract: OBJECTIVE
  To elucidate the role of Semaphorin-3F (SEMA3F), originally described as an axon guiding chemorepulsant implicated in nerve development, in the progression of colorectal carcinoma.
  
  
  METHODS
  SEMA3F and its receptor NRP2 were examined in 72 cases of human colorectal carcinoma specimens and cell lines LoVo, SW480, and SW620 with immunohistochemistry and Western blotting. SEMA3F mRNA expression in the frozen tissue specimens and cell lines was examined with quantitative reverse transcriptase-PCR. Confocal laser scanning microscopy was used for detection of cellular localization of the proteins by immunofluorescent staining. MTT assay, flow cytometry, cell adhesion and migration, and xenografts were used to evaluate biological significance of SEMA3F.
  
  
  RESULTS
  SEMA3F was significantly reduced in colorectal carcinoma tissues and cell lines. Overexpression of SEMA3F resulted in reduced proliferation, adhesion to fibronectin, and migratory capability as well as reduced S-phase population and integrin αvβ3 expression of SW480 colon cancer cells. In addition, SEMA3F-overexpressing cells exhibited diminished tumorigenesis when transplanted orthotopically in nude mice and reduced liver metastases. Moreover, transfection of siRNA targeting SEMA3F in colon cancer cells increased their tumorigenicity in vivo.
  
  
  CONCLUSIONS
  Endogenous SEMA3F acts as a suppressor of the growth and metastasis of human colorectal cancer cells.
authors.affiliation: Department of Oncology, Institute of Pathology, Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Guang-jie
authors.initials: GJ
authors.lastname: Duan
authors.forename: Juan-juan
authors.initials: JJ
authors.lastname: Ou
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.forename: Qiu-ping
authors.initials: QP
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
authors.forename: Yi-fang
authors.initials: YF
authors.lastname: Ping
authors.forename: You-hong
authors.initials: YH
authors.lastname: Cui
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.forename: Xiao-chu
authors.initials: XC
authors.lastname: Yan
authors.forename: Xiu-wu
authors.initials: XW
authors.lastname: Bian
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D045744:Cell Line, Tumor; D002464:Cell Migration Inhibition / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D015316:Genetic Therapy; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: D002630:Chemotactic Factors; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C491230:SEMA3F protein, human