title: Three 2-oxoglutarate-dependent dioxygenase activities of Equisetum arvense L. forming flavone and flavonol from (2S)-naringenin.
abstract: Equisetum arvense L. (Equisetaceae-horsetail) accumulates various flavones and flavonols in infertile shoot. Enzyme assays conducted with crude extracts of the green tissue revealed chalcone synthase activity and also three further activities assigned to flavonoid biosynthesis and identified as flavone synthase I, flavanone 3β-hydroxylase and flavonol synthase. The latter three activities were characterized as soluble, 2-oxoglutarate-dependent dioxygenases by their typical cofactor requirements and peculiar inhibition. Notably, this is the first report of flavone synthase I which had been considered to be restricted solely to species of the Apiaceae from a distant plant taxon.
authors.affiliation: Philipps-Universität Marburg, Institut für Pharmazeutische Biologie, Deutschhausstr. 17A, D-35037 Marburg, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Bredebach
authors.forename: Ulrich
authors.initials: U
authors.lastname: Matern
authors.forename: Stefan
authors.initials: S
authors.lastname: Martens
mesh_terms: D049308:Dioxygenases / Q000378:metabolism*; D020829:Equisetum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D044950:Flavanones / Q000378:metabolism*; D047309:Flavones / Q000096:biosynthesis*; D044948:Flavonols / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D044950:Flavanones; D047309:Flavones; D044948:Flavonols; D007656:Ketoglutaric Acids; D006899:Mixed Function Oxygenases; D049308:Dioxygenases; C065998:flavone synthase I; C005273:naringenin; C043562:flavone