title: Immunoblot analysis of sera from patients and vaccinees.
abstract: This chapter will describe the use of the immunoblotting method for analysing antibody specificities towards meningococcal antigens in sera from vaccinees, receiving group B meningococcal outer-membrane vesicle (OMV) vaccines, or from patients, falling ill with meningococcal disease. The power of the blotting technique lies in the simultaneous identification of many immunogenic components in a sample. Within the meningococcal field, this method has been employed in analyzes of antibody specificities of sera from vaccinees (1-6), patients (7-10), and carriers (11,12) as well as for characterization of OMV vaccines (13,14). Some of these studies present various ways of quantifying the blotting results (5,7,9,11).
authors.affiliation: Department of Vaccinology, National Institute of Public Health, Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Wedege