title: Differential expression of adipose tissue proteins between obesity-susceptible and -resistant rats fed a high-fat diet.
abstract: One of the major questions in the field of obesity is why some humans become obese (obesity prone, OP) and others resist the development of obesity (obesity resistant, OR) when exposed to a high-calorie diet, which has not been completely studied. Therefore, in the present study, in order to gain insight into the molecular mechanisms underlying this propensity, we have performed a comparative analysis of protein expression profiles in white adipose tissue (WAT) and brown adipose tissue (BAT) of rats fed a high-fat diet by 2-DE and MALDI-TOF-MS. Protein mapping of homogenates revealed significant alterations to a number of proteins; 60 and 70 proteins were differentially regulated in BAT and WAT, respectively. For careful interpretation of proteomic results, we categorized the identified proteins into two groups by analysis of both average spot density of pooled six rat adipose tissues and individual spot density of each adipose tissue of six rats as a function of body weight. One of the most striking findings of this study was that significant changes of Ehd1 and laminin receptor in BAT as well as antiquitin, DJ-1 protein, and paraoxonase 2 in WAT were found for the first time in obese rats. In addition, we confirmed the increased expression of some thermogenic enzymes and decreased lipogenic enzymes in adipose tissues of OR rats by immunoblot analysis. To our knowledge, this is the first proteomic study of profiling of protein modulation in OP and OR rats, thereby providing the first global evidence for different propensities to obesity between OP and OR rats.
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Korea.
authors.forename: Jeong In
authors.initials: JI
authors.lastname: Joo
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Oh
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Duk Kwon
authors.initials: DK
authors.lastname: Choi
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D002001:Adipose Tissue, Brown / Q000737:chemistry* / Q000378:metabolism; D052436:Adipose Tissue, White / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D043303:Aryldialkylphosphatase / Q000096:biosynthesis / Q000302:isolation & purification; D004041:Dietary Fats / Q000008:administration & dosage* / Q000378:metabolism; D004198:Disease Susceptibility / Q000378:metabolism; D015151:Immunoblotting; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D009765:Obesity / Q000378:metabolism*; D040081:Protein Array Analysis; D000071617:Protein Deglycase DJ-1; D011506:Proteins / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D017474:Receptors, Laminin / Q000737:chemistry; D033921:Vesicular Transport Proteins / Q000737:chemistry
chemical_list: D004041:Dietary Fats; C549917:EHD1 protein, rat; D008869:Microtubule-Associated Proteins; D011506:Proteins; D017474:Receptors, Laminin; D033921:Vesicular Transport Proteins; C484224:Aldh7a1 protein, rat; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1; D043303:Aryldialkylphosphatase