title: Cellular integration and vascularisation promoted by a resorbable, particulate-leached, cross-linked poly(ε-caprolactone) scaffold.
abstract: Flexible, strong scaffolds were created by crosslinking PCL with 1,6-hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5-200 µm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non-inflammatory regenerative host response, with complete infiltration by alternatively-activated CD68(+) macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti-inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events.
authors.affiliation: Jack Birch Unit of Molecular Carcinogenesis, Department of Biology, University of York, York, UK.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Baker
authors.forename: Geraldine
authors.initials: G
authors.lastname: Rohman
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hinley
authors.forename: Jens
authors.initials: J
authors.lastname: Stahlschmidt
authors.forename: Neil R
authors.initials: NR
authors.lastname: Cameron
authors.forename: Jennifer
authors.initials: J
authors.lastname: Southgate
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement; D024042:Collagen Type I / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry; D003485:Cyanates / Q000737:chemistry; D005353:Fibronectins / Q000378:metabolism; D048091:Guided Tissue Regeneration; D006801:Humans; D020007:Implants, Experimental; D017953:Isocyanates; D008863:Microspheres; D018919:Neovascularization, Physiologic; D010232:Paraffin; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D013046:Specific Gravity; D017154:Stromal Cells / Q000502:physiology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015703:Antigens, CD; D024042:Collagen Type I; D003432:Cross-Linking Reagents; D003485:Cyanates; D005353:Fibronectins; D017953:Isocyanates; D011091:Polyesters; C015262:1,6-hexamethylene diisocyanate; C016240:polycaprolactone; D010232:Paraffin