title: Hexylacrylate-based mixed-mode monolith, a stationary phase for the nano-HPLC separation of structurally related enkephalins.
abstract: The potential of an in situ photopolymerized hexylacrylate-based monolithic stationary phase-bearing sulfonic acid groups was investigated by studying the chromatographic retention of small structurally related peptides (enkephalins) by nano-LC. Several retention mechanisms were highlighted. First, a reverse-phase chromatographic behavior toward neutral solutes due to hexylacrylate-moieties was demonstrated. Second, an evaluation of the influences of buffer pH suggested the involvement of a cation-exchange mechanism due to the presence of 2-acrylamido-2-methyl-1-propanesulfonic acid. This cation-exchange phenomenon was confirmed by the clear influence of Na(+) concentration in the mobile phase on peptide retention.
authors.affiliation: Univ. Paris-Sud, UMR CNRS 8612, LPNSS, Faculté de Pharmacie, 92296 Châtenay-Malabry, France.
authors.forename: Ayat
authors.initials: A
authors.lastname: Abbood
authors.forename: Christine
authors.initials: C
authors.lastname: Herrenknecht
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Proczek
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Descroix
authors.forename: Jordi
authors.initials: J
authors.lastname: Rodrigo
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.forename: Claire
authors.initials: C
authors.lastname: Smadja
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004745:Enkephalins / Q000737:chemistry*; D015394:Molecular Structure; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D018377:Neurotransmitter Agents / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D004745:Enkephalins; D018377:Neurotransmitter Agents; D012117:Resins, Synthetic