title: FRET in cell biology: still shining in the age of super-resolution?
abstract: Interest in imaging of Förster resonance energy transfer (FRET) in biological systems has been steadily increasing in the last 30 years. The ability to transduce a near-field interaction into a far-field signal has provided a unique optical tool to assess biological phenomena well below the resolution of standard optical microscopy. In recent years, sub-diffraction microscopy techniques have achieved maturation and are increasingly used in biological applications. As the resolution of these methods increases they will slowly encroach on the domains where FRET is now dominant. Herein we review the major applications in biological FRET imaging and we discuss the possibilities and challenges in the super-resolution era.
authors.affiliation: Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany. grecco@mpi-dortmund.mpg.de
authors.forename: Hernán E
authors.initials: HE
authors.lastname: Grecco
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Verveer
mesh_terms: D019295:Computational Biology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D011506:Proteins; D020543:Proteome; D034741:RNA, Small Interfering