title: Translation-independent localization of mRNA in E. coli.
abstract: Understanding the organization of a bacterial cell requires the elucidation of the mechanisms by which proteins localize to particular subcellular sites. Thus far, such mechanisms have been suggested to rely on embedded features of the localized proteins. Here, we report that certain messenger RNAs (mRNAs) in Escherichia coli are targeted to the future destination of their encoded proteins, cytoplasm, poles, or inner membrane in a translation-independent manner. Cis-acting sequences within the transmembrane-coding sequence of the membrane proteins are necessary and sufficient for mRNA targeting to the membrane. In contrast to the view that transcription and translation are coupled in bacteria, our results show that, subsequent to their synthesis, certain mRNAs are capable of migrating to particular domains in the cell where their future protein products are required.
authors.affiliation: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University Faculty of Medicine, Post Office Box 12272, Jerusalem 91120, Israel.
authors.forename: Keren
authors.initials: K
authors.lastname: Nevo-Dinur
authors.forename: Anat
authors.initials: A
authors.lastname: Nussbaum-Shochat
authors.forename: Sigal
authors.initials: S
authors.lastname: Ben-Yehuda
authors.forename: Orna
authors.initials: O
authors.lastname: Amster-Choder
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D048168:Escherichia coli K12 / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009876:Operon; D014176:Protein Biosynthesis / Q000187:drug effects; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D001426:Bacterial Proteins; C079638:BglF protein, E coli; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D027981:Symporters; C064872:antiterminator proteins, Bacteria; D002701:Chloramphenicol; D015500:Chloramphenicol O-Acetyltransferase; D011494:Protein Kinases; D001617:beta-Glucosidase; C100305:kasugamycin