title: [Screening and application of phosphate-dissolving microorganism suitable for corn production].
abstract: OBJECTIVE
  To obtain phosphate-dissolving strains which can be suitable for corn production.
  
  
  METHODS
  We screened phosphate-dissolving strains from soil samples. We conducted the plate experiment and soil experiment in laboratory, pot experiment in greenhouse and field experiment to test the insoluble phosphate dissolving ability of the strains.
  
  
  RESULTS
  Fifteen strains of phosphate-dissolving fungi were isolated from soil samples, including 9 Penicillium oxalicum, 2 Penicillium variabile, 1 Penicillium aculeatum, 1 Trichoderma viride, 1 Aspergillus niger, and 1 Aspergillus nidulans. The 15 strains could obviously drop the pH in culture media. Five strains (Z15+, ZQ3, ZI1, Zh and Z30) reduced the pH value from 7.0 to below 2.0 in 18 h. The plate experiment and soil experiment in laboratory indicated that these 5 strains could propagate in plates and soil by using the root secretion of corn as sole carbon source. The pot experiment inoculating with the 5 strains was conducted in greenhouse by planting corn. The result demonstrated that the content of available phosphorous was increased remarkably with strains ZI1 and Zh. The content of available phosphorous at 49th day increased 28.05% and 37.04%, respectively, than initial content in strains ZI1 and Zh treatment. The dry matter yield of corn significantly increased 26.04% and 20.21% than the control, respectively. Then strains ZI1 and Zh were used in field experiment as microbial inoculums. The yield of corn treated with strain Zh increased 13.22% than that of the control, reaching 10873.05 kg/ha. However, the yield of corn treated with strain ZI1 presented no significant difference with the control.
  
  
  CONCLUSIONS
  The phosphate-dissolving strain Zh obtained in this experiment was identified as Aspergillus nidulans, which is suitable for corn production.
authors.affiliation: Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning, Beijing 100081, China. gongcaas@163.com
authors.forename: Mingbo
authors.initials: M
authors.lastname: Gong
authors.forename: Binquan
authors.initials: B
authors.lastname: Fan
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Jin
authors.forename: Guocai
authors.initials: G
authors.lastname: Wei
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Wang
mesh_terms: D001233:Aspergillus nidulans / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000737:chemistry*; D012988:Soil Microbiology; D012995:Solubility; D003313:Zea mays / Q000254:growth & development*
chemical_list: D010710:Phosphates