title: Promotion of vesicular stomatitis virus fusion by the endosome-specific phospholipid bis(monoacylglycero)phosphate (BMP).
abstract: Vesicular stomatitis virus (VSV) is a prototypic virus commonly used in studies of endocytosis and membrane trafficking. One proposed mechanism for VSV entry involves initial fusion with internal vesicles of multivesicular endosomes followed by back-fusion of these vesicles into the cytoplasm. One feature of endosomal internal vesicles is that they contain the lipid bis(monoacylglycero)phosphate (BMP). Here, we show that the presence of BMP significantly increases the rate of VSV G-mediated membrane fusion. The increased fusion was selective for VSV and was not evident for another enveloped virus, influenza virus. Our data provide a biological rationale for a two-step infection reaction during VSV entry, and suggest that BMP preferentially affects the ability of VSV G to mediate lipid mixing during membrane fusion.
authors.affiliation: Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Shoshannah L
authors.initials: SL
authors.lastname: Roth
authors.forename: Gary R
authors.initials: GR
authors.lastname: Whittaker
mesh_terms: D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000737:chemistry; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism* / Q000821:virology; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism*; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000378:metabolism; D050178:Monoglycerides / Q000737:chemistry / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000378:metabolism; D053586:Virus Internalization
chemical_list: C028391:G protein, vesicular stomatitis virus; D008055:Lipids; D008081:Liposomes; D008246:Lysophospholipids; D008562:Membrane Glycoproteins; D050178:Monoglycerides; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D014759:Viral Envelope Proteins; C012786:bis(monoacylglyceryl)phosphate; C050294:1-palmitoyl-2-oleoylglycero-3-phosphoserine; D002784:Cholesterol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine