title: Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles.
abstract: This study investigates steady-state level of bone morphogenetic protein-15 (BMP-15) mRNA in caprine follicles, and the effects of BMP-15 on in vitro development of preantral follicles. Ovarian fragments were cultured for one or seven days in Minimal Essential Medium (MEM(+)) with BMP-15 (0, 1, 10, 50, 100 or 200 ng/mL), and further analyzed by histology, transmission electron and fluorescent microscopy. BMP-15 mRNA in secondary follicles was higher than in primordial and primary follicles. After seven days, 10, 50 or 100 ng/mL of BMP-15 maintained the percentage of normal follicles similar to the control (non-cultured), and increased the oocyte and follicle diameters when compared to the control and MEM(+). BMP-15 at 100 ng/mL increased the secondary follicles and maintained their ultrastructural integrity. In conclusion, the BMP-15 mRNAs were detected in all follicular categories. BMP-15 (100 ng/mL) maintained the integrity and promoted the growth of caprine preantral follicles cultured for seven days.
authors.affiliation: Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil. juliana_jales@yahoo.com.br
authors.forename: J J H
authors.initials: JJ
authors.lastname: Celestino
authors.forename: I B
authors.initials: IB
authors.lastname: Lima-Verde
authors.forename: J B
authors.initials: JB
authors.lastname: Bruno
authors.forename: M H T
authors.initials: MH
authors.lastname: Matos
authors.forename: R N
authors.initials: RN
authors.lastname: Chaves
authors.forename: M V A
authors.initials: MV
authors.lastname: Saraiva
authors.forename: C M G
authors.initials: CM
authors.lastname: Silva
authors.forename: L R
authors.initials: LR
authors.lastname: Faustino
authors.forename: R
authors.initials: R
authors.lastname: Rossetto
authors.forename: C A P
authors.initials: CA
authors.lastname: Lopes
authors.forename: M A M
authors.initials: MA
authors.lastname: Donato
authors.forename: C A
authors.initials: CA
authors.lastname: Peixoto
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006041:Goats / Q000254:growth & development*; D046529:Microscopy, Electron, Transmission; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D046509:Tissue Culture Techniques; D014158:Transcription, Genetic
chemical_list: D055430:Bone Morphogenetic Protein 15