title: Cytosolic delivery of LDL nanoparticle cargo using photochemical internalization.
abstract: Following cellular delivery, most drugs must escape endosomes and lysosomes and reach the cytosol to be effective. This is particularly significant for nanoparticles, which can carry a large drug payload, but typically accumulate in endosomes and lysosomes. One attractive solution is to use light-triggered release, which can provide efficient endolysosomal membrane disruption and spatiotemporal control of cytosolic release. Here, we demonstrate the cytosolic release of cargo loaded into low density lipoprotein (LDL) nanoparticles using a photochemical internalization (PCI) approach. Three types of cargo-loaded LDL nanoparticles (CLLNPs) were generated by loading fluorescent dyes via (1) intercalation in the phospholipid monolayer exterior (surface loading), (2) conjugation to the amino acids of apoB-100 protein (protein loading) or (3) reconstitution into the hydrophobic core of LDL (core loading). Fluorescence imaging demonstrated the cellular uptake of CLLNPs was mediated by the LDL receptor and resulted in CLLNPs accumulation in endosomes. When cells were co-incubated with CLLNPs and AlPcS2a (a PCI agent), laser irradiation induced efficient cytosolic release of the surface-loaded and protein-labeled cargo, whereas the core-loaded hydrophobic dye could not readily be released. Thus, PCI is a useful cytosolic release method for CLLNPs, although the loading method must be considered.
authors.affiliation: Ontario Cancer Institute and Campbell Family Cancer Research Institute, Toronto, ON, Canada.
authors.forename: Honglin
authors.initials: H
authors.lastname: Jin
authors.forename: Jonathan F
authors.initials: JF
authors.lastname: Lovell
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Kenneth
authors.initials: K
authors.lastname: Ng
authors.forename: Weiguo
authors.initials: W
authors.lastname: Cao
authors.forename: Lili
authors.initials: L
authors.lastname: Ding
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
mesh_terms: D053299:Apolipoprotein B-100 / Q000737:chemistry; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D003600:Cytosol / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007364:Intercalating Agents / Q000737:chemistry; D008027:Light; D008077:Lipoproteins, LDL / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D055668:Photochemical Processes
chemical_list: D053299:Apolipoprotein B-100; D004337:Drug Carriers; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D008077:Lipoproteins, LDL