title: Extracellular matrix functionalized microcavities to control hematopoietic stem and progenitor cell fate.
abstract: Polymeric microcavities functionalized with extracellular matrix components were used as an experimental in vitro model to investigate principles of hematopoietic stem and progenitor cell (HSPC) fate control. Using human CD133+ HSPC we could demonstrate distinct differences in HSPC cycling and differentiation dependence on the adhesion ligand specificity (i.e., heparin, collagen I) and cytokine levels. The presented microcavity platform provides a powerful in vitro approach to explore the role of exogenous cues in HSPC fate decisions and can therefore be instrumental to progress in stem cell biology and translational research toward new therapies.
authors.affiliation: Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Dresden, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Kurth
authors.forename: Katja
authors.initials: K
authors.lastname: Franke
authors.forename: Tilo
authors.initials: T
authors.lastname: Pompe
authors.forename: Martin
authors.initials: M
authors.lastname: Bornhäuser
authors.forename: Carsten
authors.initials: C
authors.lastname: Werner
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D010455:Peptides / Q000032:analysis; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D012825:Silicon / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D011108:Polymers; D006493:Heparin; D003094:Collagen; D012825:Silicon