title: Antiproliferative activity of three methoxylated flavonoids isolated from Zeyheria montana Mart. (Bignoniaceae) leaves.
abstract: The present study isolated three major active flavonoids, two flavones named 4',5,7-trimethoxy-luteolin (1) and 6-hydroxy-5,7-dimethoxyflavone (2) and the flavanone 5-hydroxy-6,7-dimethoxyflavanone (3) from Zeyheria montana dichloromethane leaf extract. Isolation and purification were conducted with the application of column chromatography and structures were assigned by spectral analysis. All compounds were evaluated for cytotoxic activities against human tumor cell lines UACC-62 (melanoma), MCF-7 (breast), NCI-ADR/RES (breast expressing phenotype multiple drug resistance), 786-0 (renal), NCI-H460 (lung, non-small cells), PC-3 (prostate), OVCAR-3 (ovarian), HT-29 (colon) and K562 (leukemia) in vitro. All compounds were active in different degrees on several tumor cell lines and flavanone 3 showed cytotoxicity against almost all cell lines, particularly against human NCI-ADR/RES and K562 cell lines. In conclusion, three antiproliferative compounds were isolated for the first time from Zeyheria montana and its leaves were characterized as an important source of methoxylated flavones and flavanone as potential antitumor compounds.
authors.affiliation: Universidade Estadual Paulista-UNESP, Institute of Biosciences, Department of Pharmacology Laboratory of Phytomedicines, Botucatu, SP, Brazil.
authors.forename: Leonardo Noboru
authors.initials: LN
authors.lastname: Seito
authors.forename: Ana Lucia Tasca Goiz
authors.initials: AL
authors.lastname: Ruiz
authors.forename: Debora
authors.initials: D
authors.lastname: Vendramini-Costa
authors.forename: Sirlene Valério
authors.initials: SV
authors.lastname: Tinti
authors.forename: João Ernesto
authors.initials: JE
authors.lastname: de Carvalho
authors.forename: Jairo Kenupp
authors.initials: JK
authors.lastname: Bastos
authors.forename: Luiz Claudio
authors.initials: LC
authors.lastname: Di Stasi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D029402:Bignoniaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005419:Flavonoids; D006801:Humans; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts