title: Immunoassay-based screening of polychlorinated biphenyls (PCB) in sediments: requirements for a new generation of test kits.
abstract: Polychlorinated biphenyls (PCBs) have been proposed for the inclusion in the European Water Framework Directive (WFD) priority list, currently under revision. Various screening methods have been employed for PCB determination in different environmental matrixes in the last decades, immunoassays being one of the most employed. A literature review reveals that the enzyme-linked immunosorbent assay (ELISA) is the most commonly applied immunoassay for PCB determination in the environment. However, its application to sediments is very limited. A suitability assessment of immunoassay-based analysis for PCB screening in sediments is presented in this work. The significance of available immunoassay-based test kits under the current environmental pollution scenario and their performance against the sensitivity and specificity requirements dictated by the WFD for PCB analysis in sediments is discussed. For example, current detection limits of available test kits for PCB determination in sediments may not be enough for compliance checking under the WFD. In addition, concentration expressed as Aroclor equivalents does not seem to be the way forward. A proposal for adapting available test kits in order to become more suitable tools for PCB screening in sediments is also presented in this study.
authors.affiliation: Environnement Industriel et Risques Industriels et Naturels (LGEI), Ecole des Mines d'Alés, 6 Avenue de Clavières, 30319 Ales cedex, France. javier.castro-jimenez@idaea.csic.es
authors.forename: Javier
authors.initials: J
authors.lastname: Castro-Jiménez
authors.forename: Catherine
authors.initials: C
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry*; D057230:Limit of Detection; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls