title: High dose combination pertussis toxin induces autoimmune inner ear disease in Sprague-Dawley rats.
abstract: CONCLUSIONS
  A better animal model of autoimmune inner ear disease (AIED) in Sprague-Dawley rats has been developed by combination with high dose of pertussis toxin. This study also indicated that inner ear-specific antigens can be involved in autoimmune reactions. Cell-mediated immune injury can play an important role in the induction of AIED, at least in the earlier stage.
  
  
  OBJECTIVE
  The purpose of this study was to develop a more suitable rat model that demonstrated closer resemblance to the pathophysiological process in AIED.
  
  
  METHODS
  Ninety-six female Sprague-Dawley rats were divided into four groups. They were subcutaneously immunized with crude inner ear antigen/complete Freund's adjuvant (CIEAg/CFA), or intraperitoneal injection of 500 ng pertussis toxin (PT), or injection of CIEAg/CFA+PT, or phosphate-buffered saline (PBS) alone. The auditory function, histopathology of the inner ear, and autoantibodies were examined.
  
  
  RESULTS
  Significant differences in the time course of auditory brainstem response (ABR) threshold and mean score of cellular infiltration were demonstrated in the CIEAg/CFA+PT group of animals. Missing hair cells, degeneration of the spiral ganglion cells, endolymphatic hydrops, and autoantibodies were all noted after immunization. There were no significant differences in ABR threshold or histopathology in any other group of animals.
authors.affiliation: Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. entwjkong@yahoo.com.cn
authors.forename: Wei-Jia
authors.initials: WJ
authors.lastname: Kong
authors.forename: Deng-Yuan
authors.initials: DY
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Ding
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced* / Q000276:immunology / Q000503:physiopathology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007758:Ear, Inner / Q000187:drug effects / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007759:Labyrinth Diseases / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D008297:Male; D037342:Pertussis Toxin / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D037342:Pertussis Toxin