title: [Prescription compatibility effect on the major components absorption in danshen extract and their identification].
abstract: An improved everted gut sac method was applied to the study of prescription compatibility effect on the major components in Danshen extracts. With the separation and detection by HPLC-ECD, 5 major peaks could be detected in intestinal absorbed solution after prescription administration. Following the identification by HPLC-MS/MS, peak 2, 3, 4, and 5 were rosmaric acid, lithospermic acid, salvianolic acid B, and salvianolic acid A, respectively, which also confirmed with reference standards of those components. Through paralleling substance identification, peak 2, 3, 4, and 5 could be found as the major components in Danshen extracts, except Salvianolic acid E which is undetectable in intestinal solution. The contents of peak 2, 3, and 4 did not show difference before and after compatible prescription administrated, where the peak 5 had a significant increase in the same process. Those results revealed that peak 5, salvianolic acid A, might lead to an increasing pharmacological effect after prescription compatibility.
authors.affiliation: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Jian-xun
authors.initials: JX
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chao-yi
authors.initials: CY
authors.lastname: Mao
authors.forename: Chang-ling
authors.initials: CL
authors.lastname: Duan
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Fu
authors.forename: Cheng-ren
authors.initials: CR
authors.lastname: Lin
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000032:analysis / Q000493:pharmacokinetics; D002109:Caffeic Acids / Q000032:analysis / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D053630:Depsides / Q000032:analysis / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007773:Lactates / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001572:Benzofurans; D002109:Caffeic Acids; D053630:Depsides; D004365:Drugs, Chinese Herbal; D007773:Lactates; C046833:lithospermic acid; C028972:dan-shen root extract; C066201:salvianolic acid A; C076944:salvianolic acid B