title: Infection of human peripheral blood mononuclear cells by erythrocyte-bound HIV-1: effects of antibodies and complement.
abstract: Two human monoclonal antibodies, 4E10 and b12, were examined for antibody-dependent neutralization, or antibody-dependent complement (C)-mediated neutralization, of infection of peripheral blood mononuclear cells (PBMC) by either free HIV-1 or trans infection by HIV bound to erythrocytes. Neutralization of free HIV-1 by b12 was stronger than by 4E10, but b12 neutralized erythrocyte-bound HIV-1 less efficiently than cell-free virus. 4E10 did not neutralize erythrocyte-bound HIV-1 and at a low concentration it caused enhancement of infection. Antibody (4E10)-dependent C activation inhibited trans infection by erythrocyte-bound HIV-1, but caused enhanced infection with cell-free HIV-1 in the presence of erythrocytes. No effects of C were observed with b12. C-dependent enhancement in the presence of erythrocytes is proposed as due to binding of C3b-4E10-cell-free-HIV or C3d-4E10-cell-free-HIV to C receptor type 1 (CR1) on erythrocytes, or C receptor type 2 (CR2) on B cells in the PBMC, followed by trans infection of susceptible cells.
authors.affiliation: Division of Retrovirology, US Military HIV Research Program, Walter Reed Army Institute of Research, Rockville, MD 20850, USA.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Beck
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Brown
authors.forename: Gary R
authors.initials: GR
authors.lastname: Matyas
authors.forename: Victoria R
authors.initials: VR
authors.lastname: Polonis
authors.forename: Mangala
authors.initials: M
authors.lastname: Rao
authors.forename: Carl R
authors.initials: CR
authors.lastname: Alving
mesh_terms: D057134:Antibodies, Neutralizing / Q000276:immunology; D019067:Antibody-Dependent Enhancement; D003165:Complement System Proteins / Q000276:immunology*; D004912:Erythrocytes / Q000821:virology*; D015483:HIV Antibodies / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009500:Neutralization Tests; D053585:Virus Attachment
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D003165:Complement System Proteins