title: Molecularly imprinted beads with double thermosensitive gates for selective recognition of proteins.
abstract: A new approach is reported on the use of poly(N-isopropylacrylamide) (PNIPAM)-coated molecularly imprinted beads (coated MIP beads) for controlling the release of protein. The coated MIP beads were composed of double layers, an internal thermosensitive lysozyme-imprinted layer, and an external PNIPAM layer. The coated MIP beads were prepared by two-step surface-initiated living-radical polymerization (SIP). In this systemic study, the coated MIP beads had good selectivity to the template protein (lysozyme) and temperature stimulus-responsive behavior, both of which were superior to those of MIP beads having a layer of thermosensitive lysozyme-imprinted polymer only. Using the coated MIP beads, reference proteins and the template lysozyme could be released separately at 38 °C and at 23 °C. The corresponding coated non-imprinted beads (coated NIP beads) did not have such double thermosensitive "gates" with specific selectivity for a particular protein. The proposed smart controlled imprinted system for protein is attractive for chemical carriers, drug-delivery system, and sensors.
authors.affiliation: Department of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.forename: Xia
authors.initials: X
authors.lastname: Yuan
authors.forename: Wen-You
authors.initials: WY
authors.lastname: Li
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D054802:Molecular Imprinting / Q000295:instrumentation / Q000379:methods*; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D013696:Temperature
chemical_list: D011108:Polymers; D009113:Muramidase