title: Optimization of adsorption conditions of BSA on thermosensitive magnetic composite particles using response surface methodology.
abstract: Thermosensitive core-shell magnetic composite particles with a magnetic silica core and a rich poly (N-vinylcaprolactam) (PNVCL) shell layer were developed for studying the adsorption of bovine serum albumin (BSA) in a batch system. Various analytical and spectroscopic techniques including SEM, FT-IR, VSM and DSC were used to characterize the adsorbents prepared in this study. The combined effects of operating parameters such as initial temperature, pH and initial BSA concentration on the adsorption were analyzed using response surface methodology. The optimum conditions were 40°C, pH 4.68, and initial BSA concentration 2.0 mg/mL. Desorption experiments were conducted by altering the system temperature where a high recovery rate of protein was obtained. The separation process developed here indicates that the dual-responsive smart adsorbent could be an ideal candidate for the separation of protein.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Remin Road, Songjiang University City, Shanghai 201620, China.
authors.forename: Ming-Min
authors.initials: MM
authors.lastname: Song
authors.forename: Christopher
authors.initials: C
authors.lastname: Branford-White
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Nie
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhu
mesh_terms: D000327:Adsorption; D000818:Animals; D002209:Caprolactam / Q000031:analogs & derivatives; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature
chemical_list: D011108:Polymers; C086861:poly-N-vinylcaprolactam; D012710:Serum Albumin, Bovine; D002209:Caprolactam; D012822:Silicon Dioxide