title: In situ study of nanotemplate-induced growth of lysozyme microcrystals by submicrometer GISAXS.
abstract: Ultrasmall lysozyme microcrystals are grown by classical hanging-drop vapor diffusion and by its modification using a homologous protein thin-film template displaying long-range order. The nucleation and growth mechanisms of lysozyme microcrystals are studied at the thin lysozyme film surface using a new in situ µGISAXS (microbeam grazing-incidence small-angle X-ray scattering) technique recently developed at the microfocus beamline of the ESRF in Grenoble, France. New insight on the nucleation and crystallization processes appear to emerge.
authors.affiliation: Nanoworld Institute, University of Genoa, Corso Europa 30, 16132 Genoa, Italy.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Pechkova
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D053838:Scattering, Small Angle; D017356:Synchrotrons
chemical_list: D011506:Proteins; D009113:Muramidase