title: Cytotoxicity and effects on inflammatory response of modified types of cellulose in macrophage-like THP-1 cells.
abstract: The cytotoxicity and in vitro effects of six variously modified types of cellulose (OC--oxidized cellulose, NaOC--oxidized cellulose sodium salt, DAC--dialdehyde cellulose, CMC--carboxymethyl cellulose, MFC--microfibrilated cellulose, and MCC--microcrystalline cellulose) on the inflammatory response in macrophage-like THP-1 cells were examined, with special focus on their ability to influence gene expression and the production of TNF-α. The study provides evidence that DAC exerts a marked effect on the induction of TNF-α gene expression and its subsequent production in human macrophages. Thus, the use of DAC for anti-hemorrhagic or wound-healing therapy should be considered carefully with regard to its pro-inflammatory activity. On the contrary, MCC showed significant anti-inflammatory effects in the LPS-induced conditions, which might be beneficial for the treatment of non-healing chronic wounds, e.g., diabetic or venous ulcers.
authors.affiliation: Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, CZ-612 42 Brno, Czech Republic. kollarp@vfu.cz
authors.forename: Peter
authors.initials: P
authors.lastname: Kollar
authors.forename: Veronika
authors.initials: V
authors.lastname: Závalová
authors.forename: Jan
authors.initials: J
authors.lastname: Hošek
authors.forename: Pavel
authors.initials: P
authors.lastname: Havelka
authors.forename: Tomáš
authors.initials: T
authors.lastname: Sopuch
authors.forename: Michal
authors.initials: M
authors.lastname: Karpíšek
authors.forename: Dominika
authors.initials: D
authors.lastname: Třetinová
authors.forename: Pavel
authors.initials: P
authors.lastname: Suchý
mesh_terms: D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D007948:Leukemia, Monocytic, Acute; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051816:Tristetraprolin / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D014945:Wound Healing / Q000187:drug effects* / Q000276:immunology
chemical_list: D008070:Lipopolysaccharides; D051816:Tristetraprolin; D014409:Tumor Necrosis Factor-alpha; C497552:ZFP36 protein, human; D002482:Cellulose