title: [Solving the mysteries of the bacterial cell - application of novel techniques in fluorescence microscopy].
abstract: We have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell organization. Application of the new microscopic methods allowed localization of proteins and chromosomal regions, and observation of their migration in real time. These studies revealed the spatial organization of bacterial cells which includes specific subcellular localization of proteins, the presence of dynamic cytoskeletal structures, orchestrated and active segregation of chromosomes, and spatiotemporal gene regulation.
authors.affiliation: Wydział Biotechnologii Uniwersytetu Wrocławskiego.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Donczew
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ginda
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zakrzewska-Czerwińska
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jakimowicz
mesh_terms: D000818:Animals; D001419:Bacteria / Q000166:cytology*; D001426:Bacterial Proteins; D008164:Luminescent Proteins; D008856:Microscopy, Fluorescence
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins