title: A peptide found by phage display discriminates a specific structure of a trisaccharide in heparin.
abstract: A number of recent studies have shown that heparan sulfate can control several important biological events on the cell surface through changes in sulfation pattern. The in vivo modification of sugar chains with sulfates, however, is complicated, and the discrimination of different sulfation patterns is difficult. Heparin, which is primarily produced by mast cells, is closely approximated by the structural analog heparan sulfate. Screening of heparin-associating peptides using phage display and antithrombin-bound affinity chromatography identified a peptide, heparin-associating peptide Y (HappY), that acts as a target of immobilized heparin. The peptide consists of 12 amino acid residues with characteristic three arginines and exclusively binds to heparin and heparan sulfate but does not associate with other glycosaminoglycans. HappY recognizes three consecutive monosaccharide residues in heparin through its three arginine residues. HappY should be a useful probe to detect heparin and heparan sulfate in studies of glycobiology.
authors.affiliation: Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan. yabet@gifu-u.ac.jp
authors.forename: Tomio
authors.initials: T
authors.lastname: Yabe
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Hosoda-Yabe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kanamaru
authors.forename: Makoto
authors.initials: M
authors.lastname: Kiso
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002846:Chromatography, Affinity; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism*; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D016716:PC12 Cells; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D014312:Trisaccharides / Q000737:chemistry*
chemical_list: D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2; D006493:Heparin; D006497:Heparan Sulfate; D019151:Peptide Library; D010455:Peptides; D014312:Trisaccharides