title: Comparison between Perfadex and locally manufactured low-potassium dextran solution for pulmonary preservation in an ex vivo isolated lung perfusion model.
abstract: BACKGROUND
  Lung transplantation, a consolidated treatment for end-stage lung disease, utilizes preservation solutions, such as low potassium dextran (LPD), to mitigate ischemia-reperfusion injury. We sought the local development of LPD solutions in an attempt to facilitate access and enhance usage. We also sought to evaluate the effectiveness of a locally manufactured LPD solution in a rat model of ex vivo lung perfusion.
  
  
  METHODS
  We randomized the following groups \?\adult of male Wistar rats (n = 25 each): Perfadex (LPD; Vitrolife, Sweden); locally manufactured LPD-glucose (LPDnac) (Farmoterapica, Brazil), and normal saline solution (SAL) with 3 ischemic times (6, 12, and 24 hours). The harvested heart-lung blocks were flushed with solution at 4°C. After storage, the blocks were connected to an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus) and reperfused with homologous blood for 60 minutes. Respiratory mechanics, pulmonary artery pressure, perfusate blood gas analysis, and lung weight were measured at 10-minute intervals. Comparisons between groups and among ischemic times were performed using analysis of variance with a 5% level of significance.
  
  
  RESULTS
  Lungs preserved for 24 hours were nonviable and therefore excluded from the analysis. Those preserved for 6 hours showed better ventilatory mechanics when compared with 12 hours. The oxygenation capacity was not different between lungs flushed with LPD or LPDnac, regardless of the ischemic time. SAL lungs showed higher PCO(2) values than the other solutions. Lung weight increased over time during perfusion; however, there were no significant differences among the tested solutions (LPD, P = .23; LPDnac, P = .41; SAL, P = .26). We concluded that the LPDnac solution results in gas exchange were comparable to the original LPD (Perfadex); however ventilatory mechanics and edema formation were better with LPD, particularly among lungs undergoing 6 hours of cold ischemia.
authors.affiliation: Laboratory of Thoracic Surgery Research, Department of Cardiopneumology, InCor, Clinics Hospital, University of São Paulo, Medical School, São Paulo, Brazil.
authors.forename: P R O
authors.initials: PR
authors.lastname: Soares
authors.forename: K A D O
authors.initials: KA
authors.lastname: Braga
authors.forename: N A
authors.initials: NA
authors.lastname: Nepomuceno
authors.forename: R
authors.initials: R
authors.lastname: Pazetti
authors.forename: A T
authors.initials: AT
authors.lastname: Correia
authors.forename: P F G
authors.initials: PF
authors.lastname: Cardoso
authors.forename: F
authors.initials: F
authors.lastname: Bisceglijatene
authors.forename: P M
authors.initials: PM
authors.lastname: Pêgo-Fernandes
mesh_terms: D000818:Animals; D002951:Citrates / Q000008:administration & dosage*; D003911:Dextrans / Q000008:administration & dosage*; D008168:Lung; D008297:Male; D008962:Models, Theoretical; D011188:Potassium / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002951:Citrates; D003911:Dextrans; C070005:Perfadex; D011188:Potassium