title: Indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonasdiminuta is self-transmissible and plays a key role in horizontal mobility of the opd gene.
abstract: A fosmid library of the 66kb indigenous organophosphate-degrading (opd) plasmid pCMS1 of Brevundimonas diminuta was tagged with mini-transposon EZTn5 <R6Kγori/KAN-2>, to determine its sequence using transposon-specific primers. The sequence revealed the presence of a number of tra genes suggesting their role in conjugal transfer of pCMS1. Consistent with the presence of the tra genes, the B. diminuta plasmid, pCMS1::tet, generated by replacing the opd gene with opd::tet, served as a donor for transferring pCMS1::tet into recipient strain Pseudomonas putida. The self-transmissibility of the opd-containing plasmid pCMS1 and the existence of identical opd genes on otherwise dissimilar plasmids suggests a probable role of indigenous opd plasmids like pCMS1 in transferring the opd gene among soil bacteria.
authors.affiliation: Dept. of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: Emmanuel Vijay Paul
authors.initials: EV
authors.lastname: Pandeeti
authors.forename: Deviprasanna
authors.initials: D
authors.lastname: Chakka
authors.forename: Jay Prakash
authors.initials: JP
authors.lastname: Pandey
authors.forename: Dayananda
authors.initials: D
authors.lastname: Siddavattam
mesh_terms: D042063:Caulobacteraceae / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics; D023061:Gene Order; D022761:Gene Transfer, Horizontal; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000235:genetics