title: Age-related changes in brain mitochondrial DNA deletion and oxidative stress are differentially modulated by dietary fat type and coenzyme Q₁₀.
abstract: Mitochondria-related oxidative damage is a primary event in aging and age-related neurodegenerative disorders. Some dietary treatments, such as antioxidant supplementation or the enrichment of mitochondrial membranes with less oxidizable fatty acids, reduce lipid peroxidation and lengthen life span in rodents. This study compares life-long feeding on monounsaturated fatty acids (MUFAs), such as virgin olive oil, and n-6 polyunsaturated fatty acids, such as sunflower oil, with or without coenzyme Q₁₀ supplementation, with respect to age-related molecular changes in rat brain mitochondria. The MUFA diet led to diminished age-related phenotypic changes, with lipoxidation-derived protein markers being higher among the older animals, whereas protein carbonyl compounds were lower. It is noteworthy that the MUFA diet prevented the age-related increase in levels of mitochondrial DNA deletions in the brain mitochondria from aged animals. The findings of this study suggest that age-related oxidative stress is related, at the mitochondrial level, to other age-related features such as mitochondrial electron transport and mtDNA alterations, and it can be modulated by selecting an appropriate dietary fat type and/or by suitable supplementation with low levels of the antioxidant/electron carrier molecule coenzyme Q.
authors.affiliation: Institute of Nutrition and Food Technology José Mataix Verdú, University of Granada, 18071 Granada, Spain.
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ochoa
authors.forename: Reinald
authors.initials: R
authors.lastname: Pamplona
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Ramirez-Tortosa
authors.forename: Sergio
authors.initials: S
authors.lastname: Granados-Principal
authors.forename: Patricia
authors.initials: P
authors.lastname: Perez-Lopez
authors.forename: Alba
authors.initials: A
authors.lastname: Naudí
authors.forename: Manuel
authors.initials: M
authors.lastname: Portero-Otin
authors.forename: Magdalena
authors.initials: M
authors.lastname: López-Frías
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
authors.forename: José L
authors.initials: JL
authors.lastname: Quiles
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004042:Dietary Fats, Unsaturated / Q000032:analysis / Q000378:metabolism; D019587:Dietary Supplements; D005229:Fatty Acids, Monounsaturated / Q000032:analysis* / Q000378:metabolism; D005526:Food, Formulated; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051336:Mitochondrial Membranes / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D019636:Neurodegenerative Diseases / Q000517:prevention & control; D000069463:Olive Oil; D018384:Oxidative Stress; D010938:Plant Oils / Q000032:analysis* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D017384:Sequence Deletion; D000074242:Sunflower Oil; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D014815:Vitamins / Q000032:analysis / Q000378:metabolism; D024502:alpha-Tocopherol / Q000032:analysis / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D014815:Vitamins; D014451:Ubiquinone; C024989:coenzyme Q10; D024502:alpha-Tocopherol