title: Continuous production of 1,3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture.
abstract: The continuous production of 1,3-propanediol (1,3-PDO) was investigated with Clostridium beijerinckii NRRL B-593 using raw glycerol without purification obtained from a biodiesel production process. Ceramic rings and pumice stones were used for cell immobilization in a packed-bed bioreactor. For comparison purpose, a control bioreactor with suspended culture was also run. The effect of hydraulic retention time (HRT) on the production of 1,3-PDO in both immobilized and suspended bioreactors were also investigated. The study revealed that HRT is an important factor for both immobilized and suspended systems and a HRT of 2 h is the best one in terms of volumetric production rate (g 1,3-PDO/L/h). Furthermore, cell immobilization had also obvious benefits especially for the robustness and the reliability of the production. The results indicated that cell immobilization achieved a 2.5-fold higher productivity in comparison to suspended cell system. Based on our results, continuous production of 1,3-PDO with immobilized cells is an efficient method, and raw glycerol can be utilized without any pretreatment.
authors.affiliation: Bioengineering Department, Faculty of Engineering, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Gungormusler
authors.forename: Cagdas
authors.initials: C
authors.lastname: Gonen
authors.forename: Nuri
authors.initials: N
authors.lastname: Azbar
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D018914:Cells, Immobilized / Q000378:metabolism*; D002516:Ceramics / Q000737:chemistry; D046970:Clostridium beijerinckii / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D005285:Fermentation; D005990:Glycerol / Q000737:chemistry*; D007218:Industrial Microbiology / Q000379:methods*; D011409:Propylene Glycols / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry
chemical_list: D056804:Biofuels; D011409:Propylene Glycols; D017640:Silicates; C041787:1,3-propanediol; C005144:pumice; D005990:Glycerol