title: Designing of mouse model: a new approach for studying sulphur mustard-induced skin lesions.
abstract: This study was planned to design a mouse model for studying sulphur mustard (SM)-induced skin injury. SM was applied dermally at dose of 5 or 10 mg kg(-1) in polyethyleneglycol-300 (PEG-300) or dimethylsulphoxide (DMSO) or acetone once. The changes in body weight, organ body weight indices (OBWI) and haematological and oxidative stress parameters were investigated over a period of 3-7 days and supported by histopathological observations. Exposure to SM in PEG-300 or DMSO resulted in a significant depletion in body weight, OBWI, hepatic glutathione (GSH) and elevation in hepatic lipid peroxidation, without affecting the blood GSH and hepatic oxidised glutathione (GSSG) levels. Interestingly, no aforesaid change was observed after dermal application of SM diluted in acetone. These biochemical changes were supported by the histological observations, which revealed pronounced toxic effect and damage to liver, kidney and spleen after dermal application of SM diluted in PEG-300 or DMSO. The skin showed similar microscopic changes after dermal application of SM in all the three diluents, however; the severity of lesions was found to be time and dose dependent. It can be concluded that dermal exposure of SM diluted in acetone can be used to mimic SM-induced skin toxicity without systemic toxicity in a mouse model.
authors.affiliation: Division of Pharmacology and Toxicology, Defence R & D Establishment, Jhansi Road, Gwalior 474 002, Madhya Pradesh, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Lomash
authors.forename: Utsab
authors.initials: U
authors.lastname: Deb
authors.forename: Renuka
authors.initials: R
authors.lastname: Rai
authors.forename: Sunil E
authors.initials: SE
authors.lastname: Jadhav
authors.forename: R
authors.initials: R
authors.lastname: Vijayaraghavan
authors.forename: S C
authors.initials: SC
authors.lastname: Pant
mesh_terms: D000096:Acetone / Q000494:pharmacology; D000818:Animals; D001772:Blood Cell Count; D001835:Body Weight / Q000187:drug effects; D002057:Burns, Chemical / Q000097:blood / Q000209:etiology* / Q000473:pathology; D002619:Chemical Warfare Agents / Q000633:toxicity*; D004195:Disease Models, Animal; D005978:Glutathione / Q000032:analysis; D007668:Kidney / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009151:Mustard Gas / Q000633:toxicity*; D012867:Skin / Q000293:injuries*; D013154:Spleen / Q000473:pathology
chemical_list: D002619:Chemical Warfare Agents; D000096:Acetone; D005978:Glutathione; D009151:Mustard Gas