title: High-throughput paraffin section in situ hybridization and dual immunohistochemistry on mouse tissues.
abstract: INTRODUCTIONSection in situ hybridization (SISH) is a high-resolution tool used to analyze gene expression patterns. This protocol utilizes the Tecan Freedom EVO150 platform to perform high-throughput SISH on paraffin sections to detect mRNA with a digoxigenin (DIG)-labeled probe. The slide is mounted and imaged before performing immunohistochemistry (IHC) on the same section. The dual reaction enables a marker of protein expression to be localized on the same section as the mRNA and facilitates more accurate annotation of the gene expression.
authors.affiliation: Institute for Molecular Bioscience, University of Queensland, St. Lucia, 4072, Australia.
authors.forename: Bree
authors.initials: B
authors.lastname: Rumballe
authors.forename: Kylie
authors.initials: K
authors.lastname: Georgas
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little