title: Measurement of [Ca²+]i in whole cell suspensions using fura-2.
abstract: An elevation in intracellular calcium concentration ([Ca(2+)](i)) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization. The pattern of elevation in [Ca(2+)](i) and response to that elevation is dependent on the agonist and the cell type.
authors.affiliation: Department of Child Health, Division of Anaestheisa, Critical Care, and Pain Management, Leicester Royal Infirmary, University of Leicester, Leicester, UK.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Hirst
authors.forename: Charlotte
authors.initials: C
authors.lastname: Harrison
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hirota
authors.forename: David G
authors.initials: DG
authors.lastname: Lambert
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000378:metabolism*; D016257:Fura-2 / Q000378:metabolism*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism*; D013535:Suspensions
chemical_list: D005456:Fluorescent Dyes; D013535:Suspensions; D002118:Calcium; D016257:Fura-2