title: [Establishment and application of standard serum proteomic pattern for diagnosis of early liver cancer].
abstract: OBJECTIVE
  To establish standard serum proteomic pattern for early liver cancer and explore its value in early liver cancer diagnosis.
  
  
  METHODS
  The serum samples were obtained from 12 healthy subjects and 12 patients with early liver cancer. After removal of serum albumin and IgG, 300 µg serum sample was mixed with the hydrating solution for two-dimensional gel electrophoresis. Each experiment was repeated for 3 times. With silver staining and image analysis, the differentially expressed proteins were identified to establish the standard proteomic pattern for early liver cancer. The proteomics pattern was then tested in the diagnosis of 13 cases of chronic liver disease and 10 healthy individuals in a double-blind manner.
  
  
  RESULTS
  Thirty-eight differentially expressed protein spots were identified. Eleven protein spots with differential expressions by over 5 folds were analyzed to establish the standard proteomics pattern for early liver cancer, which showed a sensitivity of 92% and specificity of 90% in early diagnosis of liver cancer.
  
  
  CONCLUSIONS
  The standard serum 2-DE proteomic pattern for early liver cancer shows distinct differences from that of healthy subjects. The differentially expressed proteins and their specific combination pattern may have the potential for diagnosis and prognostic evaluation of early liver cancer.
authors.affiliation: Department of Infectious Disease, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. crlmczx@yahoo.com.cn
authors.forename: Ren
authors.initials: R
authors.lastname: Chen
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Chen
authors.forename: Qiu-Qiong
authors.initials: QQ
authors.lastname: Lin
authors.forename: Bing-Liang
authors.initials: BL
authors.lastname: Lin
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Cao
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D055088:Early Detection of Cancer / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D020543:Proteome