title: Cleavage at met-x bonds by cyanogen bromide.
abstract: INTRODUCTIONSelective cleavage of a protein by cyanogen bromide generates a small number of relatively large peptide fragments. This protocol describes protein cleavage with cyanogen bromide, using ~100-fold molar excess of cyanogen bromide over the methionine. The reaction is performed under mild acidic conditions (e.g., 70% formic acid), under nitrogen, and in the dark. All reagents are volatile and can be readily removed by lyophilization.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson