title: Pancreatic enzyme deficiency depends on dietary protein origin in milk-fed calves.
abstract: In young mammals, milk proteins and their substitutes are used in milk formula. Protein substitution modifies diet digestibility and pancreatic secretions. The aim of this study was to test if milk protein substitution could generate pancreatic deficiency in milk-fed calves. The effect of pancreatic juice on the digestibility of proteins was studied. Measurement of apparent fecal nutrient digestibility was used to estimate digestion. Ten calves (60 to 130 d old) were chronically fitted with pancreatic accessory duct cannulas and 2 duodenal cannulas to provide precise measurement, sampling, and reintroduction of pancreatic juice as well as additional infusions. Animals were fed milk formula based on skim milk powder or soybean concentrate. Level of deficiency depended on dietary protein origin. Twice as much protein or trypsin was required with a soybean concentrate diet than with a skim milk powder diet to obtain maximal nutrient digestibility. Pancreatic protein concentration in the juice can be used to differentiate between normal and deficient animals. Among these proteins, trypsin measurement is a good pancreatic deficiency marker. These results confirmed the major role of exocrine pancreatic secretions in producing optimal digestion in young calves. Furthermore, practical applications of these results can be applied for the young in other animal species and in humans.
authors.affiliation: INRA, UMR 1079, Système d'Elevage, Nutrition Animale et Humaine (SENAH), Domaine de la Prise, 35590 Saint-Gilles, France. Paul.Guilloteau@rennes.inra.fr
authors.forename: P
authors.initials: P
authors.lastname: Guilloteau
authors.forename: M
authors.initials: M
authors.lastname: Plodari
authors.forename: V
authors.initials: V
authors.lastname: Romé
authors.forename: G
authors.initials: G
authors.lastname: Savary
authors.forename: L
authors.initials: L
authors.lastname: Le Normand
authors.forename: R
authors.initials: R
authors.lastname: Zabielski
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle / Q000378:metabolism / Q000502:physiology*; D004063:Digestion / Q000502:physiology; D005243:Feces / Q000737:chemistry; D008297:Male; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000008:administration & dosage / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D010189:Pancreatic Juice / Q000201:enzymology; D030262:Soybean Proteins / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D008894:Milk Proteins; D030262:Soybean Proteins