title: Fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish.
abstract: BACKGROUND
  Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood.
  
  
  RESULTS
  In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp(+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli(+)etsrp(+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp(+) cell numbers at the LPM.
  
  
  CONCLUSIONS
  This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.
authors.affiliation: Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America. cchun@mcw.edu
authors.forename: Chang Zoon
authors.initials: CZ
authors.lastname: Chun
authors.forename: Indu
authors.initials: I
authors.lastname: Remadevi
authors.forename: Marcus-Oliver
authors.initials: MO
authors.lastname: Schupp
authors.forename: Ganesh Vinayak
authors.initials: GV
authors.lastname: Samant
authors.forename: Kallal
authors.initials: K
authors.lastname: Pramanik
authors.forename: George Albert
authors.initials: GA
authors.lastname: Wilkinson
authors.forename: Ramani
authors.initials: R
authors.lastname: Ramchandran
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000196:embryology* / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D004625:Embryo, Nonmammalian; D004730:Endothelium, Vascular / Q000196:embryology / Q000378:metabolism / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006257:Head / Q000196:embryology; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D008954:Models, Biological; D018919:Neovascularization, Physiologic / Q000235:genetics / Q000502:physiology; D051789:Proto-Oncogene Protein c-fli-1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013623:Tail / Q000196:embryology; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: C509408:Etv2 protein, zebrafish; D051789:Proto-Oncogene Protein c-fli-1; D029961:Zebrafish Proteins