title: Biflavanone-kolaviron protects human dopaminergic SH-SY5Y cells against atrazine induced toxic insult.
abstract: Atrazine (ATR) is a widespread agrochemical contaminant frequently detected in water systems and kolaviron (KV) is a seed-derived biflavonoid which is reported to modulate the effects of many mutagens and carcinogens. We investigated the protective effects of KV on ATR-induced cell death in the human neuroblastoma cell line (SHY-SY5Y). KV prevents ATR-induced generation of reactive oxygen species (ROS), cell death and inhibited cell proliferation by reduction of cell proliferation. Further, ATR-induced levels malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) activities, increased leakage of lactate dehydrogenase (LDH), inhibited cellular LDH activity and depleted glutathione (GSH) levels in SHY-SY5Y cells were blocked by KV. Comparable to the control, KV increased GR but not GSH-Px activities. ATR mediated nuclear changes associated with apoptosis; including nuclear fragmentation, condensation, DNA laddering, and increased caspase-3 activity were blocked on addition of KV. ATR-induced changes in the expressions of p53, Bax, Bcl-2, p21, and mRNA levels of caspase-3 and caspase-9 were prevented by KV. Based on these results, we propose a model for the protective effect of KV on ATR-induced cell injury in neuronal cell.
authors.affiliation: Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, Redemption City, Ogun State, Nigeria.
authors.forename: S O
authors.initials: SO
authors.lastname: Abarikwu
authors.forename: E O
authors.initials: EO
authors.lastname: Farombi
authors.forename: A B
authors.initials: AB
authors.lastname: Pant
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001280:Atrazine / Q000633:toxicity*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D005419:Flavonoids; D006540:Herbicides; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; C045492:kolaviron; D001280:Atrazine