title: The measurement of three cytokine transcripts in naïve and sensitized ovine peripheral blood mononuclear cells following in vitro stimulation with bluetongue virus serotype-23.
abstract: Bluetongue (BT), a serious disease of sheep and some wild ruminants, is caused by bluetongue virus (BTV), a member of the family, Reoviridae. The current research thrust for controlling BT is on development of efficient vaccines, necessitating clear understanding of ovine immunology. At present, comparative studies on cytokine gene expression profiles of naïve and BTV-sensitized peripheral blood mononuclear cells (PBMC) in sheep have not been clearly understood. In the present study, PBMC from naïve and BEI-inactivated-saponin-adjuvanted BTV-1 vaccinated sheep were stimulated in vitro with heterologous BTV-23. At various intervals, RT-qPCR was carried out to estimate cytokine (interferon-gamma, interleukin-12 and interleukin-2) mRNA expressions that are linked to cell-mediated immunity. The results showed that PBMC cytokine profiles were relatively increased both temporally and quantitatively in immunized sheep PBMC compared to naïve ones, suggesting that BTV-1 vaccination may prime immune system that can cross-react with BTV-23 antigens.
authors.affiliation: Center for Animal Disease Research and Diagnosis, Pathology Laboratory, Indian Veterinary Research Institute, Izatnagar 243122, India. chs221@mail.usask.ca
authors.forename: Channakeshava Sokke
authors.initials: CS
authors.lastname: Umeshappa
authors.forename: Karam Pal
authors.initials: KP
authors.lastname: Singh
authors.forename: Khawaja Ashfaque
authors.initials: KA
authors.lastname: Ahmed
authors.forename: Awadh Bihari
authors.initials: AB
authors.lastname: Pandey
authors.forename: Roopa Hebbandi
authors.initials: RH
authors.lastname: Nanjundappa
mesh_terms: D000818:Animals; D001820:Bluetongue virus; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000276:immunology*; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D012756:Sheep
chemical_list: D016207:Cytokines