title: Adipose-derived stem cells as a new therapeutic modality for ageing skin.
abstract: Stem cells are undifferentiated cells, which have the important properties of self-renewal and differentiation. Adipose-derived stem cells (ADSC) have relative advantages in accessibility and abundance compared to other kinds of stem cells. Regeneration therapy using ADSC has received attention in the treatment of various dermatologic diseases. In previous studies, ADSC were shown to have antioxidant, whitening and wound-healing effects in the skin through secretion of growth factors and by activating fibroblasts. In this study, we investigated whether ADSC could be used as an anti-ageing therapy, especially by dermal collagen synthesis and angiogenesis. Subcutaneous injection of ADSC significantly increased collagen synthesis in hairless mice, and dermal thickness, collagen density and fibroblast number also increased. In addition, procollagen type I protein and mRNA expression increased, which accounts for the increased dermal collagen density. Angiogenesis, which was visualized by CD31 and NG2 immunofluorescence stains, also increased in ADSC-treated skin. Our results suggest that ADSC therapy may be useful in ageing skin. Its effects are mainly mediated by stimulating collagen synthesis in dermal fibroblasts and increasing angiogenesis.
authors.affiliation: Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju, Korea.
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Kim
authors.forename: Minyoung
authors.initials: M
authors.lastname: Jung
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Yong-Man
authors.initials: YM
authors.lastname: Kim
authors.forename: Eung-Ho
authors.initials: EH
authors.lastname: Choi
mesh_terms: D052436:Adipose Tissue, White / Q000166:cytology*; D053687:Adult Stem Cells / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D002452:Cell Count; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D020405:Dermis / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D018919:Neovascularization, Physiologic / Q000502:physiology; D020286:Pericytes / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012038:Regeneration; D015595:Skin Aging; D033581:Stem Cell Transplantation / Q000379:methods; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D024042:Collagen Type I; D053509:Matrix Metalloproteinase 13; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4