title: Clinically unexpected cyclosporine levels using the ACMIA method on the RXL dimension analyser.
abstract: Safe use of cyclosporine (CsA) in solid organ transplantation relies on regular whole-blood drug monitoring. Several promising immmunoassays, e.g. the antibody-conjugated magnetic immunoassay (ACMIA) method, were developed and commercialized during recent years to compete with liquid chromatography coupled to tandem mass spectrometry, which remains the reference method but is labor-intensive. We describe the occurrence of interference in the monitoring of whole-blood CsA after transplantation when using the ACMIA method and discuss the potential mechanisms involved in such interference. Clinically unexpected results of whole-blood CsA require immediate reassessment by another technique to prevent the risk of CsA underdosage and graft rejection.
authors.affiliation: Department of Nephrology, Université Catholique de Louvain, Cliniques Universitaires Saint-Luc, Brussels, Belgium. johann.morelle@uclouvain.be
authors.forename: Johann
authors.initials: J
authors.lastname: Morelle
authors.forename: Pierre
authors.initials: P
authors.lastname: Wallemacq
authors.forename: Olivier
authors.initials: O
authors.lastname: Van Caeneghem
authors.forename: Eric
authors.initials: E
authors.lastname: Goffin
mesh_terms: D016572:Cyclosporine / Q000097:blood* / Q000276:immunology; D016903:Drug Monitoring; D016027:Heart Transplantation; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007166:Immunosuppressive Agents / Q000097:blood* / Q000276:immunology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine