title: Viral shedding duration of pandemic influenza A H1N1 virus during an elementary school outbreak--Pennsylvania, May-June 2009.
abstract: We report shedding duration of 2009 pandemic influenza A (pH1N1) virus from a school-associated outbreak in Pennsylvania during May through June 2009. Outbreak-associated students or household contacts with influenza-like illness (ILI) onset within 7 days of interview were recruited. Nasopharyngeal specimens, collected every 48 hours until 2 consecutive nonpositive tests, underwent real-time reverse transcriptase polymerase chain reaction (rRT-PCR) and culture for pH1N1 virus. Culture-positive specimens underwent virus titrations. Twenty-six (median age, 8 years) rRT-PCR-positive persons, for pH1N1 virus, were included in analysis. Median shedding duration from fever onset by rRT-PCR was 6 days (range, 1-13) and 5 days (range, 1-7) by culture. Following fever resolution virus was isolated for a median of 2 days (range, 0-5). Highest and lowest virus titers detected, 2 and 5 days following fever onset, were 3.2 and 1.2 log(10) TCID(50)/mL respectively. Overall, shedding duration in children and adults were similar to seasonal influenza viruses.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. abhattarai@cdc.gov
authors.forename: Achuyt
authors.initials: A
authors.lastname: Bhattarai
authors.forename: Julie
authors.initials: J
authors.lastname: Villanueva
authors.forename: Rakhee S
authors.initials: RS
authors.lastname: Palekar
authors.forename: Ryan
authors.initials: R
authors.lastname: Fagan
authors.forename: Wendy
authors.initials: W
authors.lastname: Sessions
authors.forename: Jörn
authors.initials: J
authors.lastname: Winter
authors.forename: Lashondra
authors.initials: L
authors.lastname: Berman
authors.forename: James
authors.initials: J
authors.lastname: Lute
authors.forename: Rebecca
authors.initials: R
authors.lastname: Leap
authors.forename: Tiffany
authors.initials: T
authors.lastname: Marchbanks
authors.forename: Samir V
authors.initials: SV
authors.lastname: Sodha
authors.forename: Mària
authors.initials: M
authors.lastname: Moll
authors.forename: Xiyan
authors.initials: X
authors.lastname: Xu
authors.forename: Alicia
authors.initials: A
authors.lastname: Fry
authors.forename: Anthony
authors.initials: A
authors.lastname: Fiore
authors.forename: Stephen
authors.initials: S
authors.lastname: Ostroff
authors.forename: David L
authors.initials: DL
authors.lastname: Swerdlow
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009305:Nasopharynx / Q000821:virology; D010414:Pennsylvania / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012574:Schools; D013997:Time Factors; D017201:Virus Shedding; D055815:Young Adult