title: [Establishment of isolated rabbit airway smooth muscles responsiveness model for the pharmacodynamic study of anti-rhinoviruses drugs].
abstract: Human rhinoviruses (HRVs) are the causative pathogens in more than half of viral upper respiratory tract infections. Currently, no antiviral agents that are active against HRVs are available for clinical use. Because only higher primates are susceptible to HRVs, the screening of new drug is most commonly based on the cell line model. In this study, isolated rabbit airway smooth muscles (ASM) tissue model has been established, and the airway responsiveness with different treatment has been examined. Relative to control tissues, the maximal constrictor (Tmax) response to ACh increased significantly 150% in ASM inoculated with HRV, and relaxation to isoproterenol has been attenuated to 63%. And the abnormal responsiveness can be inhibited in presence of pretreatment with several new compounds which have been exhibited effective anti-HRV activity on cell lines. The results demonstrate that the established ASM model will be applied to screening the anti-HRVs drugs.
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.forename: Xin-qiang
authors.initials: XQ
authors.lastname: Lu
authors.forename: Ze-hui
authors.initials: ZH
authors.lastname: Gong
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000503:physiopathology* / Q000821:virology; D010880:Piperidines / Q000494:pharmacology; D011724:Pyridazines / Q000494:pharmacology; D011817:Rabbits; D012229:Rhinovirus; D014132:Trachea / Q000187:drug effects / Q000503:physiopathology* / Q000821:virology
chemical_list: D000998:Antiviral Agents; D010880:Piperidines; D011724:Pyridazines; C074589:pirodavir; D007545:Isoproterenol; D000109:Acetylcholine