title: Extraction and identification of isothiocyanates from broccolini seeds.
abstract: Broccolini (Brassica oleracea Italica x Alboglabra) is a cross between broccoli and kai-lan (Chinese broccoli), which contains abundant glucosinolates. The intact glucosinolates are believed to be inactive, while their hydrolysis products, such as isothiocyanates (ITCs), are found to have bacteriocidal and anticarcinogenic activities. So far, no report is available about generation of ITCs during the process of glucosinolate hydrolysis in broccolini. In this study, the hydrolysis of broccolini seed glucosinolates was investigated under controlled conditions of pH, time and temperature, and the ITCs produced were determined. The results showed that an optimum hydrolysis of glucosinolates could be achieved at a temperature of 250C, at pH 7.0, and a reaction time of eight hours. Furthermore, GC-MS analysis indicated that the extracted ITCs primarily were: 3-BITC (3-benzyl-ITC) (10.8%), 4-methylpentyl-ITC (0.5%), 1-isothiocyanato-butane (26.8%), PEITC (phenethyl-ITC) (22.6%) and SFN (sulforaphane) (19.2%).
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: Bochao
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Yang
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001937:Brassica / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D017879:Isothiocyanates / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry
chemical_list: D017879:Isothiocyanates