title: ATX-LPA axis induces expression of OPN in hepatic cancer cell SMMC7721.
abstract: Osteopontin (OPN) and autotaxin (ATX) are important chemokines involved in the survival, proliferation, migration, invasion, and metastasis of many cancer cells. The focus of the study was to investigate the relationship between OPN and ATX-lysophosphatidic acid (LPA) axis. The expression of OPN and its cellular cascades were determined by western blot and real-time quantitative transcription polymerase chain reaction (real-time PCR) analyses. Cell migration activity was determined by a Transwell-migration assay. In comparison with nontreated cells, we found that the ATX-LPA axis upregulated OPN expression by 2.91-fold in protein levels and 2.52-fold in mRNA levels. The ATX-LPA axis activates Akt and ATX/LPC-induced OPN expression in SMMC7721 cells was largely reduced by the inhibitors of phosphatidylinositol 3-kinase (PI3K)/Akt or LPA receptor. This study provides the first evidence that the induction of the OPN expression by ATX-LPA axis was mediated by the activation of Akt through LPA receptors and OPN was required for migration of SMMC7721 cells induced by ATX-LPA axis.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Rihua
authors.initials: R
authors.lastname: Zhang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Pan
authors.forename: Xiayue
authors.initials: X
authors.lastname: Huang
authors.forename: Zuhu
authors.initials: Z
authors.lastname: Huang
authors.forename: Guoxin
authors.initials: G
authors.lastname: Zhang
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D053495:Osteopontin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics / Q000378:metabolism; D043264:Phosphodiesterase I / Q000235:genetics / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D011755:Pyrophosphatases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014407:Tumor Cells, Cultured; D000099189:Lysophospholipase D
chemical_list: D008246:Lysophospholipids; D009097:Multienzyme Complexes; D053495:Osteopontin; D058539:Phosphatidylinositol 3-Kinase; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases; D051057:Proto-Oncogene Proteins c-akt; D011755:Pyrophosphatases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000099189:Lysophospholipase D; C032881:lysophosphatidic acid