title: LILBID-mass spectrometry of the mitochondrial preprotein translocase TOM.
abstract: In the present work we applied a novel mass spectrometry method termed laser-induced liquid bead ion desorption mass spectrometry (LILBID-MS) to the outer mitochondrial membrane protein translocon TOM to analyze its subunit composition and stoichiometry. With TOM core complex, purified at high pH, we demonstrate that a TOM core complex of Neurospora crassa is composed of at least two Tom40 and Tom22 molecules, respectively, and more than five small Tom subunits between 5.5 and 6.4 kDa. We show that the multiprotein complex has a total molecular mass higher than 170 depending on the number of Tom5, Tom6 and Tom7 molecules bound.
authors.affiliation: Biophysics Department, Institute of Biology, University of Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
authors.forename: Frauke
authors.initials: F
authors.lastname: Mager
authors.forename: Lucie
authors.initials: L
authors.lastname: Sokolova
authors.forename: Julia
authors.initials: J
authors.lastname: Lintzel
authors.forename: Bernhard
authors.initials: B
authors.lastname: Brutschy
authors.forename: Stephan
authors.initials: S
authors.lastname: Nussberger
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000648:ultrastructure; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002352:Carrier Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins