title: Compounds with tyrosinase inhibition, elastase inhibition and DPPH radical scavenging activities from the branches of Distylium racemosum Sieb. et Zucc.
abstract: Twenty compounds were isolated from the ethanol extract of Distylium racemosum branches and their inhibitory activities on tyrosinase, elastase and free radicals evaluated. The isolated compounds were identified as dibenzofurans (1-4), abscisic acid (5), 6'-O-galloylsalidroside (6), catechin derivatives (7-11), gallic acid derivatives (12-14), tyrosol (15), flavonoids (16-18), lupeol (19) and 1,2,3,6-tetragalloylglucose (20). For study of tyrosinase inhibition activities, when compared with arbutin (IC(50) 48.8 μg/mL), four compounds (8, 11, 13, 17) showed higher activities, with IC(50) values of 4.8, 30.2, 40.5 and 37.7 μg/mL, respectively. For the elastase inhibition test, dibenzofuran 1 showed greater activity than the positive control, oleanolic acid (IC(50) 9.7 μg/mL), with an IC(50) of 7.7 μg/mL. In the studies on DPPH radical scavenging activities, five compounds (11, 12, 13, 14, 15) showed higher activities than ascorbic acid (IC(50) 5.0 μg/mL), with IC(50) values of 4.6, 3.9, 2.9, 3.8 and 4.7 μg/mL, respectively.
authors.affiliation: Jeju Bio-Industry Development Center, Jeju Technopark, Ara-1, Jeju, Korea.
authors.forename: Ryeo Kyeong
authors.initials: RK
authors.lastname: Ko
authors.forename: Gi-Ok
authors.initials: GO
authors.lastname: Kim
authors.forename: Chang-Gu
authors.initials: CG
authors.lastname: Hyun
authors.forename: Duk Sang
authors.initials: DS
authors.lastname: Jung
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D029201:Hamamelidaceae / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems; D012867:Skin / Q000201:enzymology; D013552:Swine
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; D014442:Monophenol Monooxygenase; D010196:Pancreatic Elastase