title: Bioactive surface modification on amide-photografted poly(3-hydroxybutyrate-co-3-hydroxyvalerate).
abstract: Collagen was chemically immobilized on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films via hydrophilic polyacrylamide spacers, aiming to establish the bioactive surface and the inner surface models. The inner surface modified films presented higher wettability than the surface modified films. Wide-angle x-ray diffraction results showed that the d-spacing values of the inner surface model increased compared with those of the surface model, but there was no significant difference between the amide- and collagen-modified PHBV films. The peak melting temperatures of PHBV and the special endotherm around 70 °C were following the order: PHBV > amide-modified PHBV > collagen-modified PHBV. The weight loss of the collagen-modified PHBV (inner surface model) might involve hydrolyzation and mineralization during 360 days of incubation, with a maximum value of 18.24%, while PHBV films did not show significant weight loss. The pH value of the degradation fluids fluctuated in the range of 6.86-7.22, as the initial pH was recorded at 7.20. Based on the surface model, collagen-modified PHBV scaffolds were prepared, which enhanced chondrocyte adhesion and spread on the biomimetic surface. Two surface modification models might develop a protocol with a view to generating a biocompatible and biomechanical scaffold for use in meniscus regeneration.
authors.affiliation: Institute of Life and Health Engineering, Jinan University, Guangzhou, People's Republic of China. lisa6863@163.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Ke
authors.forename: Ying Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Xue
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000577:Amides / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011091:Polyesters / Q000737:chemistry*; D012756:Sheep; D013499:Surface Properties; D013696:Temperature; D013710:Tendons / Q000473:pathology; D014961:X-Ray Diffraction; D000099166:Polyacrylamides; D000094122:Polyhydroxybutyrates
chemical_list: D000180:Acrylic Resins; D000577:Amides; D001672:Biocompatible Materials; D003094:Collagen; D011091:Polyesters; D000099166:Polyacrylamides; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates