title: Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.
abstract: OBJECTIVE
  The purpose of this study was to track and investigate the effects of autologous bone marrow-derived mesenchymal stem cells (MSCs) transplantation after acute myocardial infarction in swine assessed by magnetic resonance imaging (MRI).
  
  
  METHODS
  Twenty-four Chinese mini-pigs (27±3 kg) were divided into 4 groups, including control groups (groups 1 and 3) and MSCs transplantation groups (group 2, super paramagnetic iron oxide labeled and group 4, 4',6-diamidino-2-phenylindole labeled). Super paramagnetic iron oxide-labeled and 4',6-diamidino-2-phenylindole-labeled MSCs (3.0×10⁶ cells/mL) with a volume of 10 mL were injected into the left anterior descending artery by a catheter at 1 week after acute myocardial infarction, respectively. Cell distribution, cardiac functions, and scar tissue were quantitatively assessed by MRI.
  
  
  RESULTS
  The reduction of the T2* value in the myocardium, spleen, and liver in group 2 was significantly greater than that in group 1. MRI showed that function and scar size at baseline and 3 days after cell infusion were not significantly different between groups 1 and 2. Six weeks later left ventricular ejection fraction (P<0.0001), end-systolic volume (P<0.05), the number of dyskinetic segments (P<0.0001), left ventricular weight index (P<0.0001), and the infarcted size (P<0.0001) in group 4 were all improved comparing with those in group 3.
  
  
  CONCLUSIONS
  The majority of MSCs entrapped by the extracardial organs were mainly in the spleen. Catheter-based delivery of autologous bone marrow-derived MSCs into infarcted myocardium is feasible and effective.
authors.affiliation: Department of Radiology, Fuwai Hospital, Peking Union Medical College, People's Republic of China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Lu
authors.forename: Shihua
authors.initials: S
authors.lastname: Zhao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Liu
authors.forename: Shiliang
authors.initials: S
authors.lastname: Jiang
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qian
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Ling
authors.forename: Chaowu
authors.initials: C
authors.lastname: Yan
authors.forename: Huaibing
authors.initials: H
authors.lastname: Cheng
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003911:Dextrans / Q000494:pharmacology; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D018613:Microscopy, Confocal; D009203:Myocardial Infarction / Q000628:therapy*; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D018709:Statistics, Nonparametric; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous
chemical_list: D003911:Dextrans; D007211:Indoles; D058185:Magnetite Nanoparticles; C007293:DAPI; C065507:ferumoxides