title: Antiproliferative activity of melanoidins isolated from heated potato fiber (potex) in glioma cell culture model.
abstract: Potex constitutes a potato fiber preparation widely used as an ingredient to meat and bakery products which thermal treatment results in creation of new compounds. Melanoidins are high molecular weight brown end products of Maillard reaction, and few data presenting tumor cell growth inhibiting activity of melanoidins have been reported. Thus, in present study we utilized water extract of Potex roasted (180 °C for 2 h), whose chemical characterization revealed the presence of melanoidin complexes. Heated Potex extract inhibited C6 glioma cell proliferation in a dose-dependent manner measured by MTT method. High molecular weight components present in initial extract were responsible for stronger antiproliferative effect compared with low molecular weight fraction. Impaired MAPK (mitogen-activated protein kinase) and Akt signaling was found in cells treated with the extract. Moreover, flow cytometry analyses revealed the extract to induce G1/S arrest in glioma cells. Simultaneously, Western blot analysis showed elevated levels of p21 protein with concomitant decrease of cyclin D1. In conclusion, observed antiproliferative activity of melanoidins present in heated Potex was linked to disregulated MAPK and Akt signaling pathways, as well as to cell cycle cessation. These results suggest potential application of Potex preparation as a functional food ingredient and chemopreventive agent.
authors.affiliation: Department of Medical Biology, Institute of Agricultural Medicine, Lublin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Langner
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Nunes
authors.forename: Piotr
authors.initials: P
authors.lastname: Pozarowski
authors.forename: Martyna
authors.initials: M
authors.lastname: Kandefer-Szerszeń
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Pierzynowski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004043:Dietary Fiber / Q000494:pharmacology*; D005511:Food Handling; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D008954:Models, Biological; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011108:Polymers / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D004043:Dietary Fiber; D010936:Plant Extracts; D011108:Polymers; C011908:melanoidin polymers