title: Apoptin gene transfer via modified wheat histone H4 facilitates apoptosis of human ovarian cancer cells.
abstract: Nonviral approaches have been used extensively for intracellular gene transfer and gene therapy. A modified wheat histone H4 protein, H4TL (H4-TAT-LHRH), as a protein-based gene delivery vector that was able to form stable complexes with plasmid DNA and increase gene delivery efficiency has been described previously. In this study, H4TL has been used to deliver apoptin gene into a human ovarian carcinoma cell line HO8910. After transfection, increased expression of apoptin at both mRNA and protein levels was detected in HO8910 cells, accompanied by reduced rate of growth of HO8910 cells in vitro and the loss of mitochondrial membrane potential in these cells. These data demonstrate that H4TL-mediated transfer of apoptin initiates mitochondrial death pathway in ovarian cancer cells and suggest a novel therapeutic strategy for cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Animal Science and Veterinary, Jilin University, Changchun, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D006657:Histones / Q000235:genetics*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000235:genetics; D008928:Mitochondria / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D006657:Histones; C091717:VP3 protein, Chicken anemia virus