title: Isolation of DNA and RNA.
abstract: Blood samples for most coagulation tests are collected into 3.8% trisodium citrate in a ratio of 1 part anticoagulant to 9 parts blood. Whole-blood samples for DNA isolation can be stored at -50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats-the interface between the red cells and the plasma that is seen following centrifugation of whole blood. This latter method also allows isolation of the plasma fraction.
authors.affiliation: Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free Hospital School of Medicine, London, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Perry