title: The effect of conjugating RGD into 3D alginate hydrogels on adipogenic differentiation of human adipose-derived stromal cells.
abstract: The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels significantly enhanced the adipogenic differentiation of human ASCs to exhibit higher expression levels of oil red O staining and adipogenic genes compared to those of the control group (unmodified gels). These results suggest potential applications of RGD-modified alginate gels for adipose tissue regeneration.
authors.affiliation: Department of Biomedical Science, CHA University, Seoul, Korea.
authors.forename: Sun-Woong
authors.initials: SW
authors.lastname: Kang
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Cha
authors.forename: Honghyun
authors.initials: H
authors.lastname: Park
authors.forename: Kwang-Sook
authors.initials: KS
authors.lastname: Park
authors.forename: Kuen Yong
authors.initials: KY
authors.lastname: Lee
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D053687:Adult Stem Cells / Q000166:cytology* / Q000187:drug effects; D000464:Alginates / Q000737:chemistry*; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels; D058726:Nanoconjugates; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D000464:Alginates; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D006603:Hexuronic Acids; D020100:Hydrogels; D058726:Nanoconjugates; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D020723:Glucuronic Acid