title: Effect of streptozocin-induced diabetes mellitus on expression of methionine sulfoxide reductases and accumulation of their substrates in mouse lenses.
abstract: To determine the influences of early-stage diabetes mellitus on methionine sulfoxide reductases (Msrs) expression in lenses, streptozocin (STZ)-induced diabetic mice as animal models were used in this study. The results showed that the contents of methionine sulfoxide (MetO), protein carbonyl (PC) and Malondialdehyde (MDA) in the lenses of STZ-induced diabetic mice after 14 days were significantly higher than that in the normal control, level of total sulfhydryl groups (TSH) was 60% of normal control, and mRNA expressing levels of the MsrA and MsrBs were significantly decreased compared with normal group, as was the expression of MsrB1 protein. These results suggest that STZ not only causes increased oxidative stress, but also suppresses Msr mRNA and MsrB1 protein expression during early-stage diabetes in mice. However, the mechanism remains to research.
authors.affiliation: Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Hongshan, Wuhan, Hubei 430074, People's Republic of China. cecilylee@126.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.forename: Kaixun
authors.initials: K
authors.lastname: Huang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007908:Lens, Crystalline / Q000201:enzymology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051150:Methionine Sulfoxide Reductases / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism*; D050050:Protein Carbonylation; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013311:Streptozocin
chemical_list: D008840:Microfilament Proteins; D012333:RNA, Messenger; D008315:Malondialdehyde; D013311:Streptozocin; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C520510:Msrb2 protein, mouse; C027219:methionine sulfoxide reductase