title: Constructing Sample Chambers for Fluorescence Imaging with One-Nanometer Accuracy (FIONA).
abstract: INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. This protocol provides details on constructing an inexpensive sample chamber for use in single-molecule FIONA experiments and two methods for cleaning slides and coverslips.
authors.affiliation: Biophysics Center and Physics Department, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Selvin
authors.forename: Tyler
authors.initials: T
authors.lastname: Lougheed
authors.forename: Melinda
authors.initials: M
authors.lastname: Tonks Hoffman
authors.forename: Hyokeun
authors.initials: H
authors.lastname: Park
authors.forename: Hamza
authors.initials: H
authors.lastname: Balci
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: Blehm
authors.forename: Erdal
authors.initials: E
authors.lastname: Toprak