title: Studying NB-LRR immune receptor localization by agroinfiltration transient expression.
abstract: NB-LRR immune receptors in plants play dual roles as sentries and as activators of defense. The site in the cell where these activities take place can be different for different NB-LRRs. Furthermore, recognition and defense activation can occur in distinct subcellular compartments. Therefore, determining the subcellular localization of NB-LRRs is a key step toward understanding how they function. Recent advances in confocal microscopy enable high-resolution imaging of proteins in live cells. Agroinfiltration in the Nicotiana benthamiana model plant system is a convenient way of expressing proteins for localization studies. This chapter explains how to use N. benthamiana to transiently express NB-LRRs for confocal fluorescence microscopy.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cournoyer
authors.forename: S P
authors.initials: SP
authors.lastname: Dinesh-Kumar
mesh_terms: D005456:Fluorescent Dyes / Q000378:metabolism; D007113:Immunity, Innate; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000276:immunology*; D014170:Transformation, Genetic
chemical_list: D005456:Fluorescent Dyes; D010940:Plant Proteins; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins