title: Expansion of human trophoblastic spheroids is promoted by decidualized endometrial stromal cells and enhanced by heparin-binding epidermal growth factor-like growth factor and interleukin-1 β.
abstract: Successful pregnancy in humans depends on deep invasion of the maternal decidua by extravillous trophoblast cells (EVTs), a process regulated by autocrine and paracrine signals in the decidual-trophoblast microenvironment. Here we examined whether trophoblast invasion is affected by decidual differentiation of endometrial stromal cells (ESC) and modulated locally by cytokines and growth factors. Trophoblast spheroids were generated from the EVT-derived cell line AC-1M88 and placed onto monolayers of either undifferentiated or decidualized ESC, or directly onto tissue culture surface. Co-cultures were treated with epidermal growth factor (EGF), hepatocyte growth factor, heparin-binding EGF-like growth factor (HB-EGF), interleukin-1β (IL-1β) and leukaemia inhibitory factor (LIF). Expansion of spheroids over 2-3 days was significantly enhanced by a monolayer of undifferentiated ESC compared with tissue culture surface and further increased if ESC had been decidualized. HB-EGF and IL-1β, alone or in combination with LIF, stimulated spheroid expansion but only on undifferentiated ESC. CEACAM1, an adhesion molecule implicated in trophoblast invasion, was up-regulated in AC-1M88 cells by conditioned medium from decidualized ESC, and by HB-EGF, IL-1β and LIF in combination. Treatment of ESC with HB-EGF or IL-1β increased the level of the tetraspanin CD82, a metastasis suppressor found in decidual cells at the implantation site. We suggest that decidualized ESC support trophoblast invasion by paracrine signals that may include HB-EGF, IL-1β and LIF.
authors.affiliation: Endokrinologikum Hamburg, Falkenried 88, 20251 Hamburg, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Gonzalez
authors.forename: Julia
authors.initials: J
authors.lastname: Neufeld
authors.forename: Katja
authors.initials: K
authors.lastname: Reimann
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wittmann
authors.forename: Annemarie
authors.initials: A
authors.lastname: Samalecos
authors.forename: Anja
authors.initials: A
authors.lastname: Wolf
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Bamberger
authors.forename: Birgit
authors.initials: B
authors.lastname: Gellersen
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D066257:Heparin-binding EGF-like Growth Factor; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects
chemical_list: D017077:Culture Media, Conditioned; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D053583:Interleukin-1beta; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor