title: DAPI Staining of Drosophila Embryos.
abstract: INTRODUCTIONDrosophila embryos can be stained with specific fluorescent probes or antibodies through either direct or indirect immunofluorescence. In particular, several effective probes exist for visualizing DNA. 4',6-diamidino-2-phenylindole (DAPI) is a commonly used DNA-binding dye. Because it is specific for double-stranded DNA, no prior RNase treatment is required. While the embryo staining method described here uses DAPI, other fluorescent DNA probes can be processed similarly.
authors.forename: Wendy F
authors.initials: WF
authors.lastname: Rothwell
authors.forename: William
authors.initials: W
authors.lastname: Sullivan