title: Human hepatoma cell line (HepG2) cellular response to hypothermic stress with recovery. Induction of Hsp70, Hsp60 and Hsf1 expression.
abstract: The cell response of human HepG2 cells exposed to hypothermia with rewarming was analyzed. Ultrastructural findings in hypothermic stressed cells showed swollen mitochondria, dispersed chromatin, vacuoles and ring-shape nucleolar reorganization. These changes were coupled with significative differences in the induction of Hsp60, inducible Hsp70 and monomeric Hsfl in all treated samples, but not in Hsc 70 expression. Cellular response to hypothermia could be associated with the synergistic induction of Hsp expression.
authors.affiliation: Sección de Biología Celular, Instituto de Medicina Tropical, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Alegna
authors.initials: A
authors.lastname: Rada
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Merentes
authors.forename: Marianela
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Anselmi
authors.forename: Mirian
authors.initials: M
authors.lastname: Strauss
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor / Q000378:metabolism / Q000648:ultrastructure; D018834:Chaperonin 60 / Q000096:biosynthesis* / Q000235:genetics; D003080:Cold Temperature / Q000009:adverse effects; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D000076249:Heat Shock Transcription Factors; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008928:Mitochondria / Q000648:ultrastructure; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D017694:Rewarming; D013696:Temperature; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018834:Chaperonin 60; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D050884:HSP72 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D009363:Neoplasm Proteins; D014157:Transcription Factors