title: The properties and applications of single-molecule DNA sequencing.
abstract: Single-molecule sequencing enables DNA or RNA to be sequenced directly from biological samples, making it well-suited for diagnostic and clinical applications. Here we review the properties and applications of this rapidly evolving and promising technology.
authors.affiliation: Helicos BioSciences Corporation, Building 200LL, One Kendall Square, Cambridge, MA 02139, USA. jthompson@helicosbio.com
authors.forename: John F
authors.initials: JF
authors.lastname: Thompson
authors.forename: Patrice M
authors.initials: PM
authors.lastname: Milos
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D023281:Genomics / Q000295:instrumentation / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D045663:Quantum Dots; D012313:RNA / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
chemical_list: D017931:DNA Primers; D015335:Molecular Probes; D012313:RNA; D004247:DNA; D004259:DNA-Directed DNA Polymerase