title: A reporter for dsRNA response in Neurospora crassa.
abstract: In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system in Neurospora crassa. Using the dsRNA-activated RNA-dependent RNA polymerase gene rrp-3 promoter, we created an expression construct (pRRP-3::Myc-Al-1) and introduced it into al-1(KO) mutant. The test dsRNA efficiently induced pRRP-3::Myc-Al-1 expression in the al-1(KO) mutant, resulting in conidia color switching from white to yellow. These results confirm that the dsRNA response is regulated at the transcriptional level and this reporter system can be used for future studies in dsRNA response in filamentous fungi.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Shen
authors.forename: Jinquan
authors.initials: J
authors.lastname: Sun
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Qun
authors.initials: Q
authors.lastname: He
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012330:RNA, Double-Stranded / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D013172:Spores, Fungal / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D016271:Proto-Oncogene Proteins c-myc; D012330:RNA, Double-Stranded; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; D012324:RNA-Dependent RNA Polymerase