title: [Subcellular mimical localization of the ATP synthase B subunit from Clonorchis sinensis under different conditions of cell cycling].
abstract: OBJECTIVE
  To investigate the subcellular localization of ATP synthase b subunit from Clonorchis sinensis under different conditions of Hela cell cycling, and the effect of this protein on the expression of its encoding-gene and homologous host genes.
  
  
  METHODS
  pEGFP-N1-CsATP-synt_B and the vector pEGFP-N1 were transfected into Hela cells, respectively. Transfected cells were synchronized in G0/G1 by serum starvation, G1/S, S cells by double thymidine block, and G2/M cells by thymidine-Nocodozale block. After synchronization, the subcellular localization of the expressed fusion protein was observed with a laser confocal microscope. The expression level of this fusion protein in cells was detected by flow cytometry (FCM). The expression of CsATP-synt_B and HomoATP-synt_B in different cell cycle phases accessed by RT-PCR.
  
  
  RESULTS
  FCM results indicated that in the G0/G1 phase the expression of pEGFP-N1 vector was decreased significantly, while pEGFP-N1-CsATP-synt_B expression showed an upward trend. In the other phases of cell cycle, the protein expression was similar in the above two kinds of plasmids. The intact CsATP-synt_B was expressed in mitochondria in the G0/G1, S, and G2/M phases and nucleus during G1/S phase. After the fusion proteins entered the nucleus, the mRNA expression of CsATP-synt_B and HomoATP-synt_B increased significantly.
  
  
  CONCLUSIONS
  CsATP-synt_B can be expressed in the nucleus during G1/S phase, and regulated by the cell cycle and energy requirements.
authors.affiliation: Department of Parasitology, Zhongshan Medical College of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Yu
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Feng-Yu
authors.initials: FY
authors.lastname: Hu
authors.forename: Can
authors.initials: C
authors.lastname: Huang
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Zhao
authors.forename: Chang-Ling
authors.initials: CL
authors.lastname: Ma
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zheng
authors.forename: Xu-Chu
authors.initials: XC
authors.lastname: Hu
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism*; D003004:Clonorchis sinensis / Q000166:cytology* / Q000201:enzymology*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism*; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D025261:Mitochondrial Proton-Translocating ATPases