title: A comparison of MS2-based label-free quantitative proteomic techniques with regards to accuracy and precision.
abstract: The advent of algorithms for fragmentation spectrum-based label-free quantitative proteomics has enabled straightforward quantification of shotgun proteomic experiments. Despite the popularity of these approaches, few studies have been performed to assess their performance. We have therefore profiled the precision and the accuracy of three distinct relative label-free methods on both the protein and the proteome level. We derived our test data from two well-characterized publicly available quantitative data sets.
authors.affiliation: Department of Medical Protein Research, VIB, Ghent, Belgium.
authors.forename: Niklaas
authors.initials: N
authors.lastname: Colaert
authors.forename: Joël
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Lennart
authors.initials: L
authors.lastname: Martens
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D030562:Databases, Protein; D006801:Humans; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data*; D011786:Quality Control; D053719:Tandem Mass Spectrometry / Q000592:standards* / Q000706:statistics & numerical data*
chemical_list: D011506:Proteins