title: Host-guest chemistry of dendrimer-drug complexes. 6. Fully acetylated dendrimers as biocompatible drug vehicles using dexamethasone 21-phosphate as a model drug.
abstract: Fully acetylated poly(amidoamine) (PAMAM) dendrimer was proposed as a biocompatible drug vehicle using dexamethasone 21-phosphate (Dp21) as a model drug. NMR techniques including (1)H NMR and 2D NOE NMR were used to characterize the host-guest chemistry of acetylated dendrimer/Dp21 and cationic dendrimer/Dp21 complexes. The pH-dependent micellization, complexation, and inclusion behaviors of Dp21 were observed in the presence of acetylated and cationic PAMAM dendrimers. Acetylated dendrimer only encapsulates Dp21 at acidic conditions, while cationic dendrimer can host Dp21 at both acidic and neutral conditions. The orientation of Dp21 molecules in the dendrimer cavities depends on the quaternization degree of tertiary amine groups of dendrimer and the protonation ratio of phosphate group of Dp21. A distinctive pH-dependent release behavior of Dp21 from the acetylated and nonacetylated dendritic matrix was observed: Dp21 exhibits a much slower release rate from acetylated dendrimer at lower pH conditions and a much faster release rate from nonacetylated dendrimer with decreasing pH values. Cytotoxicity studies further confirmed the biocompatibility of acetylated dendrimers, which are much safer in the delivery of therapeutics for the treatment of various diseases than nonacetylated dendrimers. The dendrimer-drug binding and release mechanisms provide a new insight for the design and optimization of biocompatible dendrimer-based drug delivery systems.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People’s Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Weng
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Cheng
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Jiahai
authors.initials: J
authors.lastname: Zhang
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Wu
authors.forename: Tongwen
authors.initials: T
authors.lastname: Xu
mesh_terms: D000107:Acetylation; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000633:toxicity; D050091:Dendrimers / Q000737:chemistry* / Q000633:toxicity; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight
chemical_list: D020099:Coated Materials, Biocompatible; D050091:Dendrimers; C104700:PAMAM Starburst; C004180:dexamethasone 21-phosphate; D003907:Dexamethasone