title: Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.).
abstract: Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In the present study, using conventional polymerase chain reaction (PCR) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying LMW-GS gene family members. Based on sequence alignment of 13 LMW-GS genes previously identified in the Chinese bread wheat variety Xiaoyan 54 and other genes available in GenBank, PCR primers were developed and assigned to conserved sequences spanning the length polymorphism regions of LMW-GS genes. After PCR amplification, 17 DNA fragments in Xiaoyan 54 were detected using capillary electrophoresis. In total, 13 fragments were identical to previously identified LMW-GS genes, and the other 4 were derived from unique LMW-GS genes by sequencing. This marker system was also used to identify LMW-GS genes in Chinese Spring and its group 1 nulli-tetrasomic lines. Among the 17 detected DNA fragments, 4 were located on chromosome 1A, 5 on 1B, and 8 on 1D. The results suggest that this marker system is useful for large-scale identification of LMW-GS genes in bread wheat varieties, and for the selection of desirable LMW-GS genes to improve the bread-making quality in wheat molecular breeding programmes.
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhang
authors.forename: Dongcheng
authors.initials: D
authors.lastname: Liu
authors.forename: Wenlong
authors.initials: W
authors.lastname: Yang
authors.forename: Kunfan
authors.initials: K
authors.lastname: Liu
authors.forename: Jiazhu
authors.initials: J
authors.lastname: Sun
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Guo
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Li
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.forename: Hongqing
authors.initials: H
authors.lastname: Ling
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D019075:Electrophoresis, Capillary; D005819:Genetic Markers / Q000235:genetics*; D005983:Glutens / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D005983:Glutens; C037302:glutenin