title: [Chemical constituents of an endophytic fungus from Annona muricata].
abstract: OBJECTIVE
  To investigate the chemical constituents of an endophytic fungus, F-31, from Annona muricata and search antitumor natural products.
  
  
  METHODS
  After scaling up, the fermentation broth and mycelia were extracted by macroporous resin and chromatographied by silica gel column, Sephadex LH-20 gel column and semi-preparative HPLC. The structures of compounds were determined by the means of extensive spectroscopic data The activity of the compounds were evaluated through MTT assay.
  
  
  RESULTS
  Six compounds were isolated from the fermentation broth and mycelia of this fungus, their structures were identified as 5-(3-hydroxybutyl)furan-2(5H)-one(1), chloranthalactone E(2), 5, 7-dimethyl-6-hydroxycoumarin(3), 1, 2, 4-triazole-(1'R, 2'R, 3'R, 4'R)-nucleosides(4), L-tryptophan(5), L-phenylalanine(6). The in vitro pharmalogical evaluation results displayed that the above compounds exhibited no inhibitory effects on the proliferation of six tumor cell lines (HCT-8, Bel-7402, BGC-823, A549, A2780 and MCF-7).
  
  
  CONCLUSIONS
  Among these obtained compounds, compound 1 was a new compound.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Biosynthesis of Natural Products, Ministry of Public Health, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, China.
authors.forename: Hanlin
authors.initials: H
authors.lastname: Ge
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
mesh_terms: D031041:Annona / Q000382:microbiology* / Q000502:physiology; D001203:Ascomycota / Q000737:chemistry* / Q000502:physiology; D045744:Cell Line, Tumor; D005285:Fermentation; D006801:Humans; D013559:Symbiosis