title: [Study of effects and mechanism of phytosterols on chronic abacterial prostatitis].
abstract: OBJECTIVE
  To investigate the inhibitory effects of phytosterols on abacterial prostatitis and discuss the possible mechanism.
  
  
  METHODS
  Xiaozhiling-induced chronic prostatitis model were used to observe the inhibitory effect of phytosterols on abacterial prostatitis. The changes of serum IL-2, IL-1beta and TNF-alpha were evaluated by enzyme-linked immunosorbent assay (ELISA). The expression of COX-2 and 5-LOX were evaluated by Western blot and immunohistochemistry.
  
  
  RESULTS
  Treated by phytosterols (150 mg x kg(-1)), the number of white blood cells in xiaozhiling-induced chronic abacterial prostatitis rats was obviously decreased, the density of lecithin corpuscle in prostatic secretion increased and closed to control group. The edema, inflammatory infiltration of prostate were partly recovered compared with model group. The proliferation of chronic prostatitis were obviously decreased in phytosterols groups compared with model group in histological sections. Phytosterols could obviously reduce the serum IL-1beta, TNF-alpha, prostate COX-2 and 5-LOX expression and improve IL-2 level.
  
  
  CONCLUSIONS
  These results demonstrated that phytosterols had good therapeutic effects on chronic abacterial prostatitis. Participation of immune regulation and inhibiting COX-2 and 5-LOX expression may be the mechanisms of action.
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. shihong_86@yahoo.com.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
authors.forename: Liyan
authors.initials: L
authors.lastname: Cheng
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Zheng
authors.forename: Linglan
authors.initials: L
authors.lastname: Tu
mesh_terms: D000818:Animals; D002908:Chronic Disease / Q000628:therapy; D004195:Disease Models, Animal; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood / Q000276:immunology; D008297:Male; D010840:Phytosterols / Q000627:therapeutic use*; D010936:Plant Extracts / Q000627:therapeutic use*; D011472:Prostatitis / Q000188:drug therapy* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
chemical_list: D053583:Interleukin-1beta; D007376:Interleukin-2; D010840:Phytosterols; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha