title: An approach for identification of phosphoproteins using the G-electrode-loading method in two-dimensional gel electrophoresis.
abstract: We assessed whether the G-electrode-loading method (GELM) was helpful in the protein analysis. GELM in 2-DE was compared with the slip-loading, the in-gel rehydration and the cup-loading in 2-DE. GELM showed the best results for protein separation. A total of 14 spots that showed an increase with GELM were analyzed by MALDI-TOF MS. In GELM, all of these spots were identified with a high score and a high sequence coverage. A membrane-associated protein was identified and determined to have phosphorylated site. These tests show that GELM has several advantages for protein analysis compared with the traditional methods.
authors.affiliation: Sales-product Department, Anatech Corporation, Tokyo, Japan. kdobashi@anatech.co.jp
authors.forename: Kaori
authors.initials: K
authors.lastname: Koga
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Minohata
mesh_terms: D000818:Animals; D004566:Electrodes; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008099:Liver / Q000166:cytology; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice; D010449:Peptide Mapping / Q000379:methods; D010750:Phosphoproteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins