title: [Identification of clinically relevant fungi using molecular techniques].
abstract: OBJECTIVE
  To apply a sequencing-based molecular method to identify clinically relevant fungi to species level.
  
  
  METHODS
  Thirty-six fungi not identifiable at a species level by a conventional approach and 39 invasive clinical samples were prospectively evaluated. The results were compared with those obtained by conventional methods.
  
  
  RESULTS
  Molecular methods allowed rapid and reliable identification of fungi at species level, including both from organisms grown in culture and those in clinical samples.
  
  
  CONCLUSIONS
  Molecular methods show clear advantages for fungal identification, including rapid identification at species level and high negative predictive value.
authors.affiliation: Servicio de Microbiología Clínica y Parasitología, Hospital Universitario Marqués de Valdecilla, Santander, España. fabiunda@yahoo.ca
authors.forename: Fabián
authors.initials: F
authors.lastname: Unda
authors.forename: Jesús
authors.initials: J
authors.lastname: Agüero
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Fariñas
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000382:microbiology*; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011446:Prospective Studies; D021521:Ribotyping / Q000379:methods*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013997:Time Factors; D014404:Tubulin / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D014404:Tubulin