title: [Protective effect of compound tianpupian (TPP) against H(2)O(2)-induced apoptosis of murine splenic lymphocytes].
abstract: The aim of this study was to explore the protective effect of compound tianpupian (TPP) against (2)O(2)-induced the apoptosis of murine splenic lymphocytes and its mechanism. The cell apoptosis rate was detected by MTT method; the cell apoptosis and mitochondrial membrance potential were detected by flow cytometry (FCM) with Annexi-V/PI double staining and JC-1 staining method, respectively; and caspase 3 relative activity was determined by colorimetry. The results indicated that after treating with (2)O(2), the absorbance value of cultured lymphocytes and the red/green ratio of JC-1 were reduced, and the apoptotic rate and caspase 3 activity were increased, coculture of (2)O(2)-treated cells with compound TPP increased the cell absorbance ratio and red/green rate of JC-1, while reduced the apoptosis rate and caspase 3 activity. It is concluded that compound TPP alleviates intracellular oxidative damages and dose-dependently inhibited apoptosis of murine splenic lymphocytes through reducing mitochondrial membrane potential and inhibiting caspase 3 activity. This suggests that compound TPP is a potential anti-apoptotic agent.
authors.affiliation: Department of Nephrology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ri-Bao
authors.initials: RB
authors.lastname: Wei
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Zhuo
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D056604:Grape Seed Extract / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000009:adverse effects; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D044945:Proanthocyanidins / Q000494:pharmacology; D029771:Rhodiola
chemical_list: D004365:Drugs, Chinese Herbal; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D006861:Hydrogen Peroxide; D053148:Caspase 3