title: Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis.
abstract: INTRODUCTIONThis protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced.
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Amber L
authors.initials: AL
authors.lastname: Wells
authors.forename: John S
authors.initials: JS
authors.lastname: Condeelis
authors.forename: Robert H
authors.initials: RH
authors.lastname: Singer
authors.forename: Daniel
authors.initials: D
authors.lastname: Zenklusen