title: Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip.
abstract: Fluorescence cross-correlation spectroscopy (FCCS) is a highly sensitive fluorescence technique with distinct advantages in many bioanalytical applications involving interaction and binding of multiple components. Due to the use of multiple beams, bulk optical FCCS setups require delicate and complex alignment procedures. We demonstrate the first implementation of dual-color FCCS on a planar, integrated optofluidic chip based on liquid-core waveguides that can guide liquid and light simultaneously. In this configuration, the excitation beams are delivered in predefined locations and automatically aligned within the excitation waveguides. We implement two canonical applications of FCCS in the optofluidic lab-on-chip environment: particle colocalization and binding/dissociation dynamics. Colocalization is demonstrated in the detection and discrimination of single-color and double-color fluorescently labeled nanobeads. FCCS in combination with fluorescence resonance energy transfer (FRET) is used to detect the denaturation process of double-stranded DNA at nanomolar concentration.
authors.affiliation: School of Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA.
authors.forename: A
authors.initials: A
authors.lastname: Chen
authors.forename: M M
authors.initials: MM
authors.lastname: Eberle
authors.forename: E J
authors.initials: EJ
authors.lastname: Lunt
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: K
authors.initials: K
authors.lastname: Leake
authors.forename: M I
authors.initials: MI
authors.lastname: Rudenko
authors.forename: A R
authors.initials: AR
authors.lastname: Hawkins
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
mesh_terms: D003116:Color; D004247:DNA / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D049329:Nanostructures / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D004247:DNA