title: Steroidal glycosides from Ruscus ponticus.
abstract: A comparative metabolite profiling of the underground parts and leaves of Ruscus ponticus was obtained by an HPLC-ESIMS(n) method, based on high-performance liquid chromatography coupled to electrospray positive ionization multistage ion trap mass spectrometry. The careful study of HPLC-ESIMS(n) fragmentation pattern of each chromatographic peak, in particular the identification of diagnostic product ions, allowed us to get a rapid screening of saponins belonging to different classes, such as dehydrated/or not furostanol, spirostanol and pregnane glycosides, and to promptly highlight similarities and differences between the two plant parts. This approach, followed by isolation and structure elucidation by 1D- and 2D-NMR experiments, led to the identification of eleven saponins from the underground parts, of which two dehydrated furostanol glycosides and one new vespertilin derivative, and nine saponins from R. ponticus leaves, never reported previously. The achieved results highlighted a clean prevalence of furostanol glycoside derivatives in R. ponticus leaves rather in the underground parts of the plant, which showed a wider structure variety. In particular, the occurrence of dehydrated furostanol derivatives, for the first time isolated from a Ruscus species, is an unusual finding which makes unique the saponins profile of R. ponticus.
authors.affiliation: Dipartimento di Scienze Farmaceutiche e Biomediche, Università degli Studi di Salerno, Via Ponte Don Melillo, I-84084 Fisciano, Italy.
authors.forename: Assunta
authors.initials: A
authors.lastname: Napolitano
authors.forename: Tamar
authors.initials: T
authors.lastname: Muzashvili
authors.forename: Angela
authors.initials: A
authors.lastname: Perrone
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Ether
authors.initials: E
authors.lastname: Kemertelidze
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry; D031443:Ruscus / Q000737:chemistry*
chemical_list: D006027:Glycosides