title: Identification of factors involved in the anti-tumor activity of carnosine on glioblastomas using a proteomics approach.
abstract: Human glioblastoma multiforme is the most malignant brain tumor in adults and is difficult to treat. Recently, it was demonstrated that the dipeptide carnosine inhibits tumor growth, but the main molecular targets are not known. Therefore, a proteomics study with glioblastoma cells treated with carnosine was performed. Two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight detected 31 proteins expressed differentially under the influence of carnosine. Finally, peptide mass fingerprinting identified the "BCL2-associated athanogene 2" and the "von Hippel-Lindau binding protein 1" among other proteins, linking the action of carnosine to protein folding and HIF-1α signalling.
authors.affiliation: Klinik und Poliklinik für Neurochirurgie, Universitätsklinikum Leipzig AöR, 04103 Leipzig, Germany,1.
authors.forename: Ansgar
authors.initials: A
authors.lastname: Asperger
authors.forename: Christof
authors.initials: C
authors.lastname: Renner
authors.forename: Mandy
authors.initials: M
authors.lastname: Menzel
authors.forename: Rolf
authors.initials: R
authors.lastname: Gebhardt
authors.forename: Jürgen
authors.initials: J
authors.lastname: Meixensberger
authors.forename: Frank
authors.initials: F
authors.lastname: Gaunitz
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002336:Carnosine / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003598:Cytoskeletal Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010449:Peptide Mapping; D017510:Protein Folding; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; C492980:BAG2 protein, human; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C108825:VBP1 protein, human; D002336:Carnosine