title: Cloning and functional characterization of TCRP1, a novel gene mediating resistance to cisplatin in an oral squamous cell carcinoma cell line.
abstract: To explore the mechanisms of chemotherapy resistance, we previously established a multi-drug resistant cell line, Tca8113/Pingyangmycin (Tca8113/PYM) and identified differential expression in known genes and ESTs using microarray analysis. From among those ESTs we have now identified a novel gene producing an mRNA of 1834 nucleotides translated into a protein having 235 amino acids. This gene was denominated as tongue cancer resistance-associated protein 1 gene (TCRP1, accession number: EF363480). We further determined its functional characteristics. The results demonstrate that TCRP1 mediates a specific resistance to cisplatin in Tca8113 cells by reducing the cisplatin-induced apoptosis. This suggests that TCRP1 might be a novel molecular target to develop agents to reverse cisplatin-induced chemoresistance.
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan, PR China.
authors.forename: Yixue
authors.initials: Y
authors.lastname: Gu
authors.forename: Shasha
authors.initials: S
authors.lastname: Fan
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Guopei
authors.initials: G
authors.lastname: Zheng
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Yu
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D003001:Cloning, Molecular; D020552:Comet Assay; D003593:Cytoplasm / Q000378:metabolism; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D011506:Proteins; C558246:tongue cancer resistance-associated protein 1, human; D002945:Cisplatin