title: Effects of combined radiofrequency radiation exposure on the cell cycle and its regulatory proteins.
abstract: The aim of this study was to investigate whether single or combined radio frequency (RF) radiation exposure has effects on the cell cycle and its regulatory proteins. Exposure of MCF7 cells to either single (837 MHz) or combined (837 and 1950 MHz) RF radiation was conducted at specific absorption rate values of 4 W/kg for 1 h. During the exposure period, the chamber was made isothermal by circulating water through the cavity. After RF radiation exposure, DNA synthesis rate and cell cycle distribution were assessed. The levels of cell cycle regulatory proteins, p53, p21, cyclins, and cyclin-dependent kinases were also examined. The positive control group was exposed to 0.5 and 4 Gy doses of ionizing radiation (IR) and showed changes in DNA synthesis and cell cycle distribution. The levels of p53, p21, cyclin A, cyclin B1, and cyclin D1 were also affected by IR exposure. In contrast to the IR-exposed group, neither the single RF radiation- nor the combined RF radiation-exposed group elicited alterations in DNA synthesis, cell cycle distribution, and levels of cell cycle regulatory proteins. These results indicate that neither single nor combined RF radiation affect cell cycle progression.
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
authors.forename: Kwan-Yong
authors.initials: KY
authors.lastname: Lee
authors.forename: Bong Cho
authors.initials: BC
authors.lastname: Kim
authors.forename: Na-Kyung
authors.initials: NK
authors.lastname: Han
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Taehong
authors.initials: T
authors.lastname: Kim
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Yun
authors.forename: Nam
authors.initials: N
authors.lastname: Kim
authors.forename: Jeong-Ki
authors.initials: JK
authors.lastname: Pack
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000528:radiation effects*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D011846:Radio Waves
chemical_list: D018797:Cell Cycle Proteins; D004247:DNA; D001973:Bromodeoxyuridine