title: Pathogen detection using a liquid array technology.
abstract: Low concentrations of microbial pathogens in pure and mixed samples were detected using a bead-based, liquid array technology. A 20-bp sequence in the 23S rRNA gene, rrl, was amplified in four microorganisms: Bacillus cereus, Escherichia coli, Salmonella enterica and Staphylococcus aureus. PCR products were positively identified with the Luminex(®) 100™ system. The system could detect very low amounts of DNA and the instrument response was proportional to the input concentration. The lower limit of detection (LLD) was determined to be 0.5 ng for B. cereus and E. coli and 2 ng for S. enterica. The LLD for S. aureus was not determined as the instrument response was still above the threshold when quantities of DNA as low as 0.25 ng were used. The platform positively identified organisms present in mixed samples even when the minor component was overshadowed by a 10-fold excess of the major component.
authors.affiliation: Department of Sciences, John Jay College of Criminal Justice, City University of New York, 445 West 59th Street, New York, NY 10019, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Battaglia
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Schweighardt
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Wallace
mesh_terms: D001409:Bacillus cereus / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005453:Fluorescence; D016680:Genome, Bacterial; D046228:Microarray Analysis; D008863:Microspheres; D016133:Polymerase Chain Reaction; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D019779:Salmonella enterica / Q000235:genetics*; D017421:Sequence Analysis; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D012338:RNA, Ribosomal, 23S