title: Serological survey of Encephalitozoon cuniculi infection in Japanese dogs.
abstract: Antibodies to Encephalitozoon cuniculi were examined by enzyme-linked immunosorbent assay using E. cuniculi PTP2 recombinant protein and by Western blot analysis on a total of 472 dog serum samples that had been collected in Japan. Of these samples, 21.8% (103/472) had antibodies against E. cuniculi. Each of 5 serum samples that showed high (>1.0) or low (<0.1) OD value was selected randomly and further examined by Western blot using E. cuniculi-native antigens. All samples with high OD values reacted with specific E. cuniculi proteins, including an antigen of approximately 35 kDa corresponding with PTP2; sera with low OD values did not recognize this E. cuniculi band. This study is the first to demonstrate the prevalence of E. cuniculi infection in dogs in Japan.
authors.affiliation: Department of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: A
authors.initials: A
authors.lastname: Yamazaki
authors.forename: A
authors.initials: A
authors.lastname: Haraguchi
authors.forename: M
authors.initials: M
authors.lastname: Tatsumi
authors.forename: K
authors.initials: K
authors.lastname: Ishida
authors.forename: H
authors.initials: H
authors.lastname: Ikadai
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D015153:Blotting, Western / Q000662:veterinary; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D009670:Encephalitozoon cuniculi / Q000276:immunology*; D016890:Encephalitozoonosis / Q000453:epidemiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005656:Fungal Proteins / Q000276:immunology; D007564:Japan / Q000453:epidemiology; D016036:Seroepidemiologic Studies
chemical_list: D000908:Antibodies, Fungal; D005656:Fungal Proteins