title: CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell.
abstract: Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.
authors.affiliation: Programa de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ, Brasil.
authors.forename: D P
authors.initials: DP
authors.lastname: Aguiar
authors.forename: J M
authors.initials: JM
authors.lastname: Coelho-Aguiar
authors.forename: J G
authors.initials: JG
authors.lastname: Abreu
mesh_terms: D002448:Cell Adhesion; D002449:Cell Aggregation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D055513:Connective Tissue Growth Factor / Q000494:pharmacology*; D054278:Embryonal Carcinoma Stem Cells / Q000187:drug effects*; D006801:Humans
chemical_list: D055513:Connective Tissue Growth Factor