title: New laboratory tools in the assessment of bone quality.
abstract: Bone quality is a complex set of intricated and interdependent factors that influence bone strength. A number of methods have emerged to measure bone quality, taking into account the organic or the mineral phase of the bone matrix, in the laboratory. Bone quality is a complex set of different factors that are interdependent. The bone matrix organization can be described at five different levels of anatomical organization: nature (organic and mineral), texture (woven or lamellar), structure (osteons in the cortices and arch-like packets in trabecular bone), microarchitecture, and macroarchitecture. Any change in one of these levels can alter bone quality. An altered bone remodeling can affect bone quality by influencing one or more of these factors. We have reviewed here the main methods that can be used in the laboratory to explore bone quality on bone samples. Bone remodeling can be evaluated by histomorphometry; microarchitecture is explored in 2D on histological sections and in 3D by microCT or synchrotron. Microradiography and scanning electron microscopy in the backscattered electron mode can measure the mineral distribution; Raman and Fourier-transformed infra-red spectroscopy and imaging can simultaneously explore the organic and mineral phase of the matrix on multispectral images; scanning acoustic microscopy and nanoindentation provide biomechanical information on individual trabeculae. Finally, some histological methods (polarization, surface staining, fluorescence, osteocyte staining) may also be of interest in the understanding of quality as a component of bone fragility. A growing number of laboratory techniques are now available. Some of them have been described many years ago and can find a new youth; others having benefited from improvements in physical and computer techniques are now available.
authors.affiliation: INSERM, U922-IRIS-IBS Institut de Biologie en Santé, CHU d'Angers, 49933, Angers, France. daniel.chappard@univ-angers.fr
authors.forename: D
authors.initials: D
authors.lastname: Chappard
authors.forename: M F
authors.initials: MF
authors.lastname: Baslé
authors.forename: E
authors.initials: E
authors.lastname: Legrand
authors.forename: M
authors.initials: M
authors.lastname: Audran
mesh_terms: D015519:Bone Density / Q000502:physiology; D016723:Bone Remodeling / Q000502:physiology; D001842:Bone and Bones / Q000648:ultrastructure; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D010024:Osteoporosis / Q000175:diagnosis*; D058866:Osteoporotic Fractures / Q000175:diagnosis