title: Proteomic characterization of the multiple forms of the PLAs from the venom of the social wasp Polybia paulista.
abstract: The phospholipases A(1) (PLA(1) s) from the venom of the social wasp Polybia paulista occur as a mixture of different molecular forms. To characterize the molecular origin of these structural differences, an experimental strategy was planned combining the isolation of the pool of PLAs from the wasp venom with proteomic approaches by using 2-D, MALDI-TOF-TOF MS and classical protocols of protein chemistry, which included N- and C-terminal sequencing. The existence of an intact form of PLA(1) and seven truncated forms was identified, apparently originating from controlled proteolysis of the intact protein; in addition to this, four of these truncated forms also presented carbohydrates attached to their molecules. Some of these forms are immunoreactive to specific-IgE, while others are not. These observations permit to raise the hypothesis that naturally occurring proteolysis of PLA(1) , combined with protein glycosylation may create a series of different molecular forms of these proteins, with different levels of allergenicity. Two forms of PLA(2) s, apparently related to each other, were also identified; however, it was not possible to determine the molecular origin of the differences between both forms, except that one of them was glycosylated. None of these forms were immunoreactive to human specific IgE.
authors.affiliation: Institute of Biosciences of Rio Claro, Department of Biology, Center of the Study of Social Insects/Dept. Biology, University of São Paulo State (UNESP), Rio Claro, SP, Brazil.
authors.forename: Lucilene Delazari
authors.initials: LD
authors.lastname: dos Santos
authors.forename: Anally Ribeiro
authors.initials: AR
authors.lastname: da Silva Menegasso
authors.forename: José Roberto Aparecido
authors.initials: JR
authors.lastname: dos Santos Pinto
authors.forename: Keity Souza
authors.initials: KS
authors.lastname: Santos
authors.forename: Fabio Morato
authors.initials: FM
authors.lastname: Castro
authors.forename: Jorge Elias
authors.initials: JE
authors.lastname: Kalil
authors.forename: Mario Sergio
authors.initials: MS
authors.lastname: Palma
mesh_terms: D000818:Animals; D006031:Glycosylation; D007073:Immunoglobulin E / Q000276:immunology; D007527:Isoenzymes / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D013058:Mass Spectrometry; D054466:Phospholipases A1 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D040901:Proteomics; D020539:Sequence Analysis, Protein; D014862:Wasp Venoms / Q000032:analysis* / Q000276:immunology; D014863:Wasps / Q000737:chemistry*
chemical_list: D007527:Isoenzymes; D014862:Wasp Venoms; D007073:Immunoglobulin E; D054466:Phospholipases A1