title: [Optimization of extraction process of total alkaloids from radix of Aconitum flavum using response surface methodology].
abstract: OBJECTIVE
  To study the technological parameters of the extraction process of total alkaloids from Radix of Aconitum flavum.
  
  
  METHODS
  Using response surface methodology (RSM) of three factors and three levels to optimize ethanol heat reflux applied for alkaloids extraction from Radix of Aconitum flavum.
  
  
  RESULTS
  A quadratic polynomial mathematical model with good correlation was constructed and employed to the optimization. The optimal extraction conditions were as follows: the extraction time was 1.72 h, the concentration of ethanol was 64.00%, the ratio of ethanol to raw material was 8.18 mL/g, under which the predictive value of the rate of extraction yield of alkaloids was 0.526% and the measured value was 0. 521%, respectively, and there was a good agreement with their predicted values.
  
  
  CONCLUSIONS
  This reveals the good predictability of the mathematical model, the selected process also has a good reproducibility.
authors.affiliation: School of Pharmaceutical Science, Lanzhou University, Lanzhou 730030, China. gaoxiaojuan0705@126.com
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Gao
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Ma
authors.forename: Lin
authors.initials: L
authors.lastname: Dong
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Ma
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Fu
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D003124:Colorimetry / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
chemical_list: D000470:Alkaloids; D000431:Ethanol