title: Phytase-active yeasts from grain-based food and beer.
abstract: OBJECTIVE
  To screen yeast strains isolated from grain-based food and beer for phytase activity to identify high phytase-active strains.
  
  
  RESULTS
  The screening of phytase-positive strains was carried out at conditions optimal for leavening of bread dough (pH 5·5 and 30°C), in order to identify strains that could be used for the baking industry. Two growth-based tests were used for the initial testing of phytase-active strains. Tested strains belonged to six species: Saccharomyces cerevisiae, Saccharomyces pastorianus, Saccharomyces bayanus, Kazachstania exigua (former name Saccharomyces exiguus), Candida krusei (teleomorph Issachenkia orientalis) and Arxula adeninivorans. On the basis of initial testing results, 14 strains were selected for the further determination of extracellular and intracellular (cytoplasmic and/or cell-wall bound) phytase activities. The most prominent strains for extracellular phytase production were found to be S. pastorianus KVL008 (a lager beer strain), followed by S. cerevisiae KVL015 (an ale beer strain) and C. krusei P2 (isolated from sorghum beer). Intracellular phytase activities were relatively low in all tested strains.
  
  
  CONCLUSIONS
  Herein, for the first time, beer-related strains of S. pastorianus and S. cerevisiae are reported as phytase-positive strains.
  
  
  CONCLUSIONS
  The high level of extracellular phytase activity by the strains mentioned previously suggests them to be strains for the production of wholemeal bread with high content of bioavailable minerals.
authors.affiliation: Department of Food Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Nuobariene
authors.forename: A S
authors.initials: AS
authors.lastname: Hansen
authors.forename: L
authors.initials: L
authors.lastname: Jespersen
authors.forename: N
authors.initials: N
authors.lastname: Arneborg
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D001515:Beer / Q000382:microbiology*; D001939:Bread / Q000382:microbiology*; D002523:Edible Grain / Q000382:microbiology; D005516:Food Microbiology; D012440:Saccharomyces / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D015003:Yeasts / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D010832:6-Phytase