title: [8-hydroxy-dihydroberberine ameliorated insulin resistance induced by high FFA and high glucose in 3T3-L1 adipocytes].
abstract: The purpose of the study is to investigate the effect of 8-hydroxy-dihydroberberine on insulin resistance induced by high free fatty acid (FFA) and high glucose in 3T3-L1 adipocytes and its possible molecular mechanism. Palmic acid or glucose in combination with insulin was used to induce insulin resistance in 3T3-L1 adipocytes. 8-Hydroxy-dihydroberberine and berberine were added to the cultured medium separately, which were considered as treated group and positive control group. The rate of glucose uptake was determined by 2-deoxy-[3H]-D-glucose method. The amount of glucose consumption in the medium was measured by glucose oxidase method. Cell growth and proliferation of 3T3-L1 adipocytes were detected with Cell Counting Kit-8 (CCK-8) assay. After incubated with palmic acid for 24 hours or glucose with insulin for 18 hours, the rate of glucose transport in 3T3-L1 adipocytes was inhibited by 67% and 58%, respectively. The amount of glucose consumption in 3T3-L1 adipose cells was decreased by 41% after cells were incubated with palmic acid for 24 h. However, the above changes were reversed by pretreatment with 8-hydroxy-dihydroberberine for 24 and 48 h. Significant difference existed between groups. Insulin resistance in 3T3-L1 adipocytes, which is induced by high FFA and high glucose, could be ameliorated by 8-hydroxy-dihydroberberine.
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Xu
authors.forename: Fu-er
authors.initials: FE
authors.lastname: Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Yi
authors.forename: Zeng-si
authors.initials: ZS
authors.lastname: Wang
authors.forename: Shi-chao
authors.initials: SC
authors.lastname: Wei
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.forename: Xin
authors.initials: X
authors.lastname: Zou
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D051379:Mice; D015394:Molecular Structure; D019308:Palmitic Acid / Q000494:pharmacology
chemical_list: C583055:8-hydroxy-dihydroberberine; D007004:Hypoglycemic Agents; D007328:Insulin; D001599:Berberine; D019308:Palmitic Acid; D005947:Glucose