title: Transperitoneal transport of uric acid: impact of p-cresol, sodium hyaluronan, and sodium deoxycholate in vitro.
abstract: Our study investigated uric acid transport across isolated parietal peritoneum taken from the anterior abdominal wall of white New Zealand rabbits and placed inside a modified Ussing-type chamber. Values for transfer from the mesothelial to the interstitial side of membrane (M --> I) were calculated using the mathematical model of mass transport and are expressed as a coefficient of diffusive permeability [P (in centimeters per second)]. Four separate series of experiments were done. In the first series, we examined uric acid transfer in control conditions (for 120 minutes). In the second and third series, P was calculated before (15 - 60 minutes) and after introduction of p-cresol (0.005 g/dL) or sodium hyaluronan (0.04 g/dL) on the M side of the membrane. In the fourth series, transfer parameters were measured before (15 - 75 minutes) and after (90 - 150 minutes) application of sodium deoxycholate (0.104 g/dL). The dynamics of transperitoneal transport of uric acid were stable. The values of P +/- standard error of the mean (x0.0001) were 1.936 +/- 0.324 cm/s and 2.078 +/- 0.186 cm/s. Application of p-cresol on the M side of membrane lowered uric acid transport by 10%. Application of sodium hyaluronan produced no change, but application of sodium deoxycholate increased the transfer of uric acid by 155%. These observations may have clinical importance.
authors.affiliation: Department of Chemistry and Clinical Biochemistry, Poznań University of Medical Sciences, Poznań, Poland. tgrzelak@ump.edu.pl
authors.forename: Teresa
authors.initials: T
authors.lastname: Grzelak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Osiewała
authors.forename: Beata
authors.initials: B
authors.lastname: Szary
authors.forename: Krystyna
authors.initials: K
authors.lastname: Czyzewska
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003408:Cresols / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D010537:Peritoneum / Q000378:metabolism*; D010539:Permeability; D011817:Rabbits; D014527:Uric Acid / Q000378:metabolism*
chemical_list: D003408:Cresols; D003840:Deoxycholic Acid; C032538:4-cresol; D014527:Uric Acid; D006820:Hyaluronic Acid