title: Mapping phosphoproteins in Neisseria meningitidis serogroup A.
abstract: To investigate the phosphorylation capability of serogroup A Neisseria meningitidis (MenA) and to implement our knowledge in meningococcal biology and in bacterial post-translational modifications, cell extracts were separated by 2-DE and 51 novel phosphoproteins were revealed by the use of the highly specific Ser/Thr/Tyr-phosphorylated proteins staining by Pro-Q Diamond and identified by MALDI-ToF/MS. Our results indicate that phosphorylation in MenA is comparable to that of other bacterial species. A first functional characterization of the identified modified proteins was also given, in order to understand their role in meningococcal physiopathology.
authors.affiliation: Dipartimento di Biologia Molecolare, Università degli Studi di Siena, via Fiorentina, Siena, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Bernardini
authors.forename: Marcella
authors.initials: M
authors.lastname: Laschi
authors.forename: Tommaso
authors.initials: T
authors.lastname: Serchi
authors.forename: Simona
authors.initials: S
authors.lastname: Arena
authors.forename: Chiara
authors.initials: C
authors.lastname: D'Ambrosio
authors.forename: Daniela
authors.initials: D
authors.lastname: Braconi
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: Annalisa
authors.initials: A
authors.lastname: Santucci
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D002457:Cell Extracts / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008585:Meningitis, Meningococcal / Q000382:microbiology; D038381:Neisseria meningitidis, Serogroup A / Q000235:genetics / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling / Q000379:methods; D014357:Trypsin / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002457:Cell Extracts; D010750:Phosphoproteins; D014357:Trypsin