title: [Comparison of six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris].
abstract: OBJECTIVE
  To compare six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris.
  
  
  METHODS
  Six molecular methods, such as 16S rRNA gene analysis, 16S-23S rRNA Intergenic spacer region polymorphism, Denaturing Gradient Gel Electrophoresis (DGGE), Random Amplified Polymorphic DNA (RAPD), Repetitive Extragenic Palindromic-PCR and Restricted Fragment Length Polymorphisms were used to differentiate the reference strains.
  
  
  RESULTS
  Except for 16S rRNA gene analysis and 16S-23S rRNA Intergenic spacer region polymorphism, the other protocols were competent.
  
  
  CONCLUSIONS
  The methods of DGGE and RAPD were more appropriate for differentiation of Lactococcus lactis subsp. lactis and cremoris.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China. zhjch321123@sohu.com
authors.forename: Jiachao
authors.initials: J
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Bao
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Sun
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
mesh_terms: D058645:Denaturing Gradient Gel Electrophoresis; D013294:Lactococcus lactis / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D012336:RNA, Ribosomal, 16S