title: Novel methylotrophic isolates from lake sediment, description of Methylotenera versatilis sp. nov. and emended description of the genus Methylotenera.
abstract: Phylogenetic positions, and genotypic and phenotypic characteristics of three novel methylotrophic isolates, strains 301(T), 30S and SIP3-4, from sediment of Lake Washington, Seattle, USA, are described. The strains were restricted facultative methylotrophs capable of growth on single carbon compounds (methylamine and methanol) in addition to a limited range of multicarbon compounds. All strains used the N-methylglutamate pathway for methylamine oxidation. Strain SIP3-4 possessed the canonical (MxaFI) methanol dehydrogenase, but strains 301(T) and 30S did not. All three strains used the ribulose monophosphate pathway for C1 assimilation. The major fatty acids in the three strains were C(16:0) and C(16:1)ω7c. The DNA G+C contents of strains 301(T) and SIP3-4 were 42.6 and 54.6 mol%, respectively. Based on 16S rRNA gene sequence phylogeny and the relevant phenotypic characteristics, strain SIP3-4 was assigned to the previously defined species Methylovorus glucosotrophus. Strains 301(T) and 30S were closely related to each other (100% 16S rRNA gene sequence similarity) and shared 96.6% 16S rRNA gene sequence similarity with a previously described isolate, Methylotenera mobilis JLW8(T). Based on significant genomic and phenotypic divergence with the latter, strains 301(T) and 30S represent a novel species within the genus Methylotenera, for which the name Methylotenera versatilis sp. nov. is proposed; the type strain is 301(T) (=VKM B-2679(T)=JCM 17579(T)). An emended description of the genus Methylotenera is provided.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA, USA.
authors.forename: Marina G
authors.initials: MG
authors.lastname: Kalyuzhnaya
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA, USA.|eScience Institute, University of Washington, Seattle, WA, USA.
authors.forename: David A C
authors.initials: DAC
authors.lastname: Beck
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Alexey
authors.initials: A
authors.lastname: Vorobev
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Smalley
authors.affiliation: Dennis Kunkel Microscopy, Inc., Box 2008 Kailua, HI 96734, USA.
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Kunkel
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA, USA.|Department of Microbiology, University of Washington, Seattle, WA, USA.
authors.forename: Mary E
authors.initials: ME
authors.lastname: Lidstrom
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Chistoserdova
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002244:Carbon / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water; D019015:Geologic Sediments / Q000382:microbiology*; D042762:Methylophilaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014861:Washington
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D002244:Carbon