title: Residue-specific fluorescent probes of α-synuclein: detection of early events at the N- and C-termini during fibril assembly.
abstract: In the Parkinson's disease-associated state, α-synuclein undergoes large conformational changes, forming ordered, β-sheet-containing fibrils. To unravel the role of specific residues during the fibril assembly process, we prepared single-Cys mutants in the disordered (G7C and Y136C) and proximal (V26C and L100C) fibril core sites and derivatized them with environmentally sensitive dansyl (Dns) fluorophores. Dns fluorescence exhibits residue specificity in spectroscopic properties as well as kinetic behavior; early kinetic events were revealed by probes located at positions 7 and 136 compared to those at positions 26 and 100.
authors.affiliation: Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, United States.
authors.forename: Thai Leong
authors.initials: TL
authors.lastname: Yap
authors.forename: Candace M
authors.initials: CM
authors.lastname: Pfefferkorn
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003619:Dansyl Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D009154:Mutation; D010300:Parkinson Disease / Q000378:metabolism; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D051844:alpha-Synuclein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D000682:Amyloid; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D051844:alpha-Synuclein