title: [Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation].
abstract: The aim of this study is to investigate the effect of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation. Duck hepatitis B virus (DHBV) infection model and normal mouse spleen lymphocyte were used to evaluate the anti-HBV and immunoregulation effects. The DHBV-DNA of serum was detected at different time points by using serum DOT-BLOT hybridization. Polymerase chain reaction (PCR) was used for the determination of nuclear covalent closed circular DNA (cccDNA). Cytokine secretion was determined by ELISA method. DHBV-DNA were inhibited by hyperin (25 or 50 mg x kg(-1)), while cccDNA of liver could be eliminated efficiently by hyperin (25 or 50 mg x kg(-1), P < 0.05, P < 0.01). The T helper 1 effector cytokine was markedly enhanced by hyperin (25 or 50 microg x mL(-1), P < 0.01). In conclusion, hyperin has anti-HBV activity via multiple targets and pathways, and cccDNA may be one of the important targets.
authors.affiliation: Institute of Geriatrics, Chinese PLA General Hospital, Beijing 100853, China. gengmiao_2000@yahoo.com
authors.forename: Miao
authors.initials: M
authors.lastname: Geng
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Chen
authors.forename: Xin-Bo
authors.initials: XB
authors.lastname: Yang
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Huang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D004270:DNA, Circular / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D018347:Hepadnaviridae Infections / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000235:genetics*; D006524:Hepatitis, Viral, Animal / Q000821:virology; D007371:Interferon-gamma / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D008099:Liver / Q000821:virology; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D013154:Spleen / Q000473:pathology / Q000821:virology
chemical_list: D000998:Antiviral Agents; D004270:DNA, Circular; D004279:DNA, Viral; D018664:Interleukin-12; D007371:Interferon-gamma; C021304:hyperoside; D011794:Quercetin