title: Phylogenetic and recombination analysis of human bocavirus 2.
abstract: BACKGROUND
  Human bocavirus 2(HBoV2) and other human bocavirus species (HBoV, HBoV3, and HBoV4) have been discovered recently. But the precise phylogenetic relationships among these viruses are not clear yet.
  
  
  METHODS
  We collected 632 diarrhea and 162 healthy children in Lanzhou, China. Using PCR, Human bocavirus (HBoV), HBoV2, HBoV3 and HBoV4 were screened. The partial genes of NS, NP1 and VP, and two nearly complete sequences of HBoV2 were obtained.
  
  
  RESULTS
  Phylogenetic analysis showed the different genes of HBoV2 strain were homogenous with different reference strains. HBoV3 may be a recombinant derived from HBoV and HBoV4. We also observed that the VP1 and VP2 region of HBoV3 is as similar to HBoV2 as to HBoV4.
  
  
  CONCLUSIONS
  A single genetic lineage of HBoV2 is circulating in children with and without gastroenteritis in Lanzhou, China. Current evidence in this study was not enough to support recombination between HBoV2 strains, and HBoV3 may be a recombinant between HBoV and the common ancestor of HBoV2 and HBoV4.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, PR China.
authors.forename: Weixia
authors.initials: W
authors.lastname: Cheng
authors.forename: Jinan
authors.initials: J
authors.lastname: Chen
authors.forename: Ziqian
authors.initials: Z
authors.lastname: Xu
authors.forename: Jiemei
authors.initials: J
authors.lastname: Yu
authors.forename: Canping
authors.initials: C
authors.lastname: Huang
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Huiying
authors.initials: H
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D002675:Child, Preschool; D002681:China; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics*; D003967:Diarrhea / Q000821:virology; D019143:Evolution, Molecular; D056661:Human bocavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins