title: [Salvianolic acid B alleviate the disruption of blood-brain barrier in rats after cerebral ischemia-reperfusion by inhibiting MAPK pathway].
abstract: The aim of the study is to investigate the effect of salvianolic acid B (SalB) on blood-brain barrier (BBB) in rats after cerebral ischemia-reperfusion, and to illustrate its possible mechanisms. Cerebral ischemia-reperfusion was induced by middle cerebral artery occlusion in rats. The break-down of BBB was indicated by extravasations of immunoglobulin (IgG) monitored with immunohistochemistry. The expression of MMP-9 and NOS2 in the brain was determined by immunohistochemistry, and the expression of p-p38 and p-ERK1/2 was detected by Western blotting. It was shown that on day 2 after ischemia-reperfusion the IgG accumulated around the vascular boundary zone, suggesting the break-down of BBB, and the expression of MMP-9 and NOS2 up-regulated at the same time. The result of Western blotting suggested that the expression of p-p38 and p-ERK1/2 increased. On day 7 after ischemia-reperfusion the. expression of MMP-9 and NOS2 was about the same level as day 2, the expression of p-p38 was higher than that on day 2 and the expression of p-ERK1/2 was slightly lower than that on day 2. SalB (1 and 10 mg x kg(-1)) significantly alleviated the extravasations of immunoglobulin induced by cerebral ischemia-reperfusion (P < 0.05). On day 2 and day 7 SalB attenuated the expression of MMP-9 and NOS2 (P < 0.05). SalB (10 mg x kg(-1)) reduced the expression of p-p38 and p-ERK1/2 apparently on day 2 and 7 after ischemia-reperfusion (P < 0.05). SalB (1 mg x kg(-1)) inhibited the expression of p-p38 on day 7 after ischemia-reperfusion (P < 0.05). The results indicate that SalB protects blood-brain barrier in rats after cerebral ischemia-reperfusion by inhibiting the MAPK pathway.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Li-pei
authors.initials: LP
authors.lastname: Han
authors.forename: Ze-hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Jun-tian
authors.initials: JT
authors.lastname: Zhang
authors.forename: Min-ke
authors.initials: MK
authors.lastname: Tang
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology; D020244:Infarction, Middle Cerebral Artery / Q000150:complications; D048728:MAP Kinase Kinase Kinase 1 / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010766:Phosphorylation; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D027561:Salvia miltiorrhiza / Q000737:chemistry; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D053630:Depsides
chemical_list: D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D048728:MAP Kinase Kinase Kinase 1; D020780:Matrix Metalloproteinase 9; D052247:Nitric Oxide Synthase Type II; D048051:p38 Mitogen-Activated Protein Kinases; C076944:salvianolic acid B; D053630:Depsides; C496318:Nos2 protein, rat