title: Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue.
abstract: Assays to determine the role of pathogen effectors within an infected plant cell are yielding valuable information about which host processes are targeted to allow successful pathogen colonization. However, this does not necessarily inform on the cellular location of these interactions, or if these effector-virulence target interactions occur only in the presence of the pathogen. Here, we describe techniques to allow the subcellular localization of pathogen effectors inside infected plant cells or tissues, based largely on infiltration of plant tissue by Agrobacterium tumefaciens and its delivery of DNA encoding fluorescent protein-tagged effectors, and subsequent confocal microscopy.
authors.affiliation: Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
authors.forename: Petra C
authors.initials: PC
authors.lastname: Boevink
authors.forename: Paul R J
authors.initials: PR
authors.lastname: Birch
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Whisson
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D018014:Gene Transfer Techniques; D054884:Host-Pathogen Interactions; D018613:Microscopy, Confocal / Q000379:methods; D010838:Phytophthora / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000382:microbiology*
chemical_list: D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins