title: Diagnostic accuracy of urine filtration and dipstick tests for Schistosoma haematobium infection in a lightly infected population of Ghanaian schoolchildren.
abstract: Two screening methods, reagent dipsticks for hematuria and urine filtration for Schistosoma haematobium eggs, were evaluated for their sensitivity and specificity in diagnosing infection with S. haematobium in lightly infected Ghanaian children. Schoolchildren aged 8-18 years (n=255) provided urine samples on three occasions. Overall, 36.4% of girls and 50.7% of boys presented with eggs at least once; 3.3% of girls and 7.5% of boys presented with both eggs and hematuria three times. Many children presented with eggs but without hematuria, or with hematuria but without eggs. When each child was screened three times, the sensitivity of each test method improved by at least 22.9% as compared with single screening, but previously unidentified infections were detected at the third screening, indicating that even three screenings is insufficient. Nearly half of lightly infected children (<50 eggs/10 ml urine, by maximum egg count) were egg-positive during only one of three screenings. Thus, data presented here indicate that when individuals are screened repeatedly, infection status can be assessed more accurately, control programs can be properly evaluated, and population estimates of S. haematobium infection may be made with increased confidence, as compared with single screening.
authors.affiliation: Department of Civil and Environmental Engineering, Tufts University, Medford, MA 02155, USA. karen.kosinski@tufts.edu
authors.forename: Karen C
authors.initials: KC
authors.lastname: Kosinski
authors.forename: Kwabena M
authors.initials: KM
authors.lastname: Bosompem
authors.forename: Miguel J
authors.initials: MJ
authors.lastname: Stadecker
authors.forename: Anjuli D
authors.initials: AD
authors.lastname: Wagner
authors.forename: Jeanine
authors.initials: J
authors.lastname: Plummer
authors.forename: John L
authors.initials: JL
authors.lastname: Durant
authors.forename: David M
authors.initials: DM
authors.lastname: Gute
mesh_terms: D000293:Adolescent; D000818:Animals; D002648:Child; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D005374:Filtration / Q000379:methods; D005869:Ghana; D006417:Hematuria / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D008853:Microscopy / Q000379:methods; D010274:Parasitology / Q000379:methods*; D012548:Schistosoma haematobium / Q000302:isolation & purification*; D012553:Schistosomiasis haematobia / Q000175:diagnosis* / Q000469:parasitology; D012680:Sensitivity and Specificity; D013334:Students; D014556:Urine / Q000469:parasitology*