title: Isolation of DNA from Mycobacterium tubercolosis.
abstract: Research into and identification of Mycobacterium tuberculosis can take on a number of facets, many of which involve the use of DNA at one stage or another. The quality and quantity of DNA required will depend on the end-use requirement. For example, good yields of pure, high-molecular-weight DNA uncontaminated by DNA from other sources (i.e., homogeneous) are optimal for the generation of cosmid libraries and sequencing (1), Southern hybridization (2-6), or microarray analysis (7) for genome studies, whereas relatively crude DNA (fragmented DNA or DNA from multiple sources [i.e., heterogeneous]) may be adequate for PCR-based diagnosis (8-12) or amplification of regions of the genome for other purposes, e.g., identification of mutations conferring drug resistance (13,14).
authors.affiliation: MRC Centre for Molecular and Cellular Biology, University of Stellenbosch Medical School, Tygerberg, South Africa.
authors.forename: P D
authors.initials: PD
authors.lastname: van Helden
authors.forename: T C
authors.initials: TC
authors.lastname: Victor
authors.forename: R M
authors.initials: RM
authors.lastname: Warren
authors.forename: E G
authors.initials: EG
authors.lastname: van Helden