title: [Preparation and quality evaluation of ginkgolide B-loaded self microemulsifying drug delivery system].
abstract: OBJECTIVE
  To prepare ginkgolide B-loaded self microemulsifying drug delivery system (SMEDDS) and evaluate its quality.
  
  
  METHODS
  The solubility of ginkgolide B in different oil, surfactant and co-surfactant were measured by HPLC-ESI-MS. The GB-SMEDDS formulation was optimized by the self emulsifying efficiency of various combinations of oil and mix-surfactant evaluated by using pseudo-temary phase diagram. The preliminary stability of GB-SEMEDDS was evaluated by the variety of loading rate of GB and dispersed medium. The morphology, the particle size and the formulation stability were evaluated after diluting by 0.1 mol x L(-1) HCl.
  
  
  RESULTS
  The blank self microemulsified system was composed of ethyl oleate-( cremophor EL-lecithin-ethanol, 4: 1:2) (40: 60), the loading dosage was 2.5%. Little influence of GB and emulsified medium was observed on the stability of GB-SEMDDS. After diluted with 0.1 mol x L(-1) HCl, the morphology of the microemulsion was homogeneous small spherical drops observed under electro-microscope. The particle size was (41.6 +/- 1.11) nm, the self microemulsifing time was around 2 min. The formulation was stable within 8 h, without significant changes in particle size and separation of drugs.
  
  
  CONCLUSIONS
  GB-SMEDDS is easy to prepare and its quality is stable. The solubility of GB was significantly improved by SMEDDS.
authors.affiliation: Department of Ophthalmology, Guangzhou Women and Children's Medical center, Guangzhou 510120, China. gmengxiang@hahoo.com.cn
authors.forename: Mengxiang
authors.initials: M
authors.lastname: Guo
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hu
authors.forename: Shibo
authors.initials: S
authors.lastname: Tang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhu
authors.forename: Yandong
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000295:instrumentation / Q000379:methods*; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D004655:Emulsions / Q000737:chemistry*; D046934:Ginkgolides / Q000737:chemistry* / Q000493:pharmacokinetics; D007783:Lactones / Q000737:chemistry* / Q000493:pharmacokinetics; D010316:Particle Size; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004655:Emulsions; D046934:Ginkgolides; D007783:Lactones; D013501:Surface-Active Agents; C045856:ginkgolide B