title: Susceptibility loci and chromosomal abnormalities in radiation induced hematopoietic neoplasms in mice.
abstract: Genetics of susceptibility to radiation-induced hematopoietic neoplasms and somatic chromosomal aberrations were analyzed in 305 backcross (CcS-17xCcS-2)xCcS-2 mice of two CcS/Dem recombinant congenic strains. Irradiated CcS-2 mice were previously shown to exhibit high frequency of myeloid neoplasms whereas irradiated CcS-17 mice were susceptible to T-cell lymphomas. Mice were exposed to four whole-body irradiation doses of 1.7 Gy at one week intervals, which resulted in 139 hematopoietic neoplasms. The hematopoietic neoplasms were classified according to the Bethesda proposals for classification of lymphoid and nonlymphoid hematopoietic neoplasms in mice. Genotyping of mice with 24 microsatellite markers and subsequent statistical analysis indicated linkage of the radiation induced T-lymphomas to two loci on chromosome 10 (D10Mit134) and chromosome 12 (D12Mit52). T-lymphoma susceptibility appeared to be linked to D10Mit134 in a sex dependent way. In contrast, the myeloid-granulocytic leukemias susceptibility is linked to combined effects of chromosome 5 (D5Mit179) and 16 (D16Mit34). Cytogenetic analysis was performed according to the standard G-bands procedure and confirmed using FISH method. We found non-random numerical and structural chromosomal changes in lymphoid neoplasms. Cytogenetic analysis indicated chromosomal aberrations presumably associated with lymphomagenesis, no specific cancer-related rearrangements were observed.
authors.affiliation: Department of Genetics and Laboratory Animal Breeding, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Piskorowska
authors.forename: Marta
authors.initials: M
authors.lastname: Gajewska
authors.forename: Hanna
authors.initials: H
authors.lastname: Szymańska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Krysiak
authors.forename: Lei
authors.initials: L
authors.lastname: Quan
authors.forename: Beata
authors.initials: B
authors.lastname: Grygalewicz
authors.forename: Henryk M
authors.initials: HM
authors.lastname: Skurzak
authors.forename: Alina
authors.initials: A
authors.lastname: Czarnomska
authors.forename: Barbara
authors.initials: B
authors.lastname: Pienkowska-Grela
authors.forename: Peter
authors.initials: P
authors.lastname: Demant
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D002875:Chromosomes / Q000528:radiation effects*; D003433:Crosses, Genetic; D020022:Genetic Predisposition to Disease; D005838:Genotype; D019337:Hematologic Neoplasms / Q000209:etiology / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats; D008957:Models, Genetic; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000235:genetics*; D040641:Quantitative Trait Loci