title: Plasma esterified and non-esterified fatty acids metabolic profiling using gas chromatography-mass spectrometry and its application in the study of diabetic mellitus and diabetic nephropathy.
abstract: Using gas chromatography-mass spectrometry (GC-MS), a new metabolic profiling method was established to assess the levels of non-esterified fatty acids (NEFAs) and esterified fatty acids (EFAs) in plasma. The extraction method was simple and robust without removing protein process. With this method 25 fatty acids (FAs), both EFAs and NEFAs, can be recognized simultaneously with only 10 μL plasma. 15 of the 25 can be precisely quantified. The method was validated and then applied into clinical metabonomics research. Five clinical groups including 150 cases were involved. The relationship between FA levels and diabetic mellitus (DM) as well as diabetic nephropathy (DN) pathology was speculated. Furthermore, the possible pathological causes and effects were discussed in detail. Potential biomarkers (p value <0.01) were screened with Student's t-test. With the application of partial least squares-discriminant analysis (PLS-DA), different stages were distinguished. The result may be useful for the pathology study of metabolic syndromes, and may also be helpful for monitoring the progression of DM and DN.
authors.affiliation: School of Pharmaceutics, East-China University of Science & Technology, Shanghai, China.
authors.forename: Li-Da
authors.initials: LD
authors.lastname: Han
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Xia
authors.forename: Qiong-Lin
authors.initials: QL
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Guo-An
authors.initials: GA
authors.lastname: Luo
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D003920:Diabetes Mellitus / Q000097:blood; D003928:Diabetic Nephropathies / Q000097:blood; D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry; D005230:Fatty Acids, Nonesterified / Q000097:blood* / Q000737:chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified