title: Structure-function analysis of the TibA self-associating autotransporter reveals a modular organization.
abstract: Some enterotoxigenic Escherichia coli strains express the TibA adhesin/invasin, a multifunctional autotransporter that mediates the autoaggregation of bacteria, biofilm formation, adhesion to cultured epithelial cells, and invasion of these cells. To elucidate the structure-function relationship in TibA, we generated mutants by transposon-based linker scanning mutagenesis and by site-directed mutagenesis. Several insertion mutants had a defect in either adhesion or autoaggregation. Mutants with a defect in autoaggregation were found in the N-terminal half of the extracellular domain, while mutants with a defect in adhesion were found in the C-terminal half. The deletion of the putative N-terminal autoaggregation domain abolished the autoaggregation of the bacteria but did not affect adhesion. The deletion of a proline-rich region located at the C terminus of the extracellular domain abolished the adhesion properties of TibA but did not affect invasion. This finding suggests that adhesion and invasion may rely on distinct mechanisms. Thus, our results reveal that TibA possesses a modular organization, with the extracellular domain being separated into an autoaggregation module and an adhesion module.
authors.affiliation: Université de Montréal, Faculté de Médecine Vétérinaire, 3200 Sicotte, Saint-Hyacinthe, Québec J2S 7C6, Canada.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Côté
authors.forename: Michael
authors.initials: M
authors.lastname: Mourez
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism* / Q000472:pathogenicity*; D015151:Immunoblotting; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D016133:Polymerase Chain Reaction; D013329:Structure-Activity Relationship
chemical_list: D018830:Adhesins, Escherichia coli; C557497:TibA protein, E coli