title: Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-D-galactopyranoside derivatives.
abstract: α-D-Galactopyranosides were synthesized and their inhibitory activities toward the Debaryomyces hansenii UFV-1 extracellular and intracellular α-galactosidases were evaluated. Methyl α-D-galactopyranoside was the most potent inhibitor compared to the others tested, with K(i)(') values of 0.82 and 1.12 mmolL(-1), for extracellular and intracellular enzymes, respectively. These results indicate that the presence of a hydroxyl group in the C-6 position of α-D-galactopyranoside derivatives is important for the recognition by D. hansenii UFV-1 α-galactosidases.
authors.affiliation: BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Pollyanna A
authors.initials: PA
authors.lastname: Viana
authors.forename: Sebastião T
authors.initials: ST
authors.lastname: de Rezende
authors.forename: Arianne de A
authors.initials: Ade A
authors.lastname: Alves
authors.forename: Rozângela M
authors.initials: RM
authors.lastname: Manfrini
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Alves
authors.forename: Marcelo P
authors.initials: MP
authors.lastname: Bemquerer
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Santoro
authors.forename: Valéria M
authors.initials: VM
authors.lastname: Guimarães
mesh_terms: D055165:Debaryomyces / Q000201:enzymology*; D005690:Galactose / Q000031:analogs & derivatives / Q000378:metabolism*; D005696:Galactosidases / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D005696:Galactosidases; D005690:Galactose