title: [Expression profiling of peroxisome proliferation activate receptor gamma2 and phosphorylation during adipogenic differentiation of adipose-derived stem cells].
abstract: OBJECTIVE
  To investigate PPARgamma2 expression and phosphorylation involved in adipogenic differentiations of Adipose-derived Stem Cells (ASCs) with an aim to reveal the possible mechanisms of adipose development.
  
  
  METHODS
  ASCs were obtained from the inguinal fat pads of GFP (green fluorescent protein) mice. The primary cells were cultured in histiocytic attachment cultivation. Adipogenic differentiation of the third generation of ASCs was induced with the conditional medium (alpha-MEM medium supplemented with 100 mL/L fetal bovine serum, 10(-6) mol/L dexamethasone, 10(-5) mol/L insulin, 0. 2 mmol/L indomethacin, and 0.5 mmol/L 3-isobutyl-1-methylxanthine). The levels of PPARgamma2 mRNA and the PPARgamma2 expression and phosphorylation at day 0, 1, 3, 5, 7, and 9 of induction were detected using RT-PCR, fluorescence-immunocytochemistry and western blot, respectively.
  
  
  RESULTS
  The expression of PPARgamma2 and phosphorylation were significantly up-regulated during adipogenic differentiation of ASCs.
  
  
  CONCLUSIONS
  PPARgamma2 is the key regulator of adipose development. The regulation of PPARgamma2 phosphorylation may promote adipogenic differentiation of ASCs.
authors.affiliation: College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Qin-ce
authors.initials: QC
authors.lastname: Sun
authors.forename: Jin-gang
authors.initials: JG
authors.lastname: Xiao
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Yang
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Tian
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D051379:Mice; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D047495:PPAR gamma