title: A reliable and durable approach for real-time determination of cellular superoxide anion based on biomimetic superoxide dismutase stabilized by a zeolite.
abstract: This paper demonstrates a reliable and durable method for in situ real-time determination of O(2)˙(-) based on direct electron transfer of Mn(3)(PO(4))(2), which acts as a superoxide dismutase (SOD). Mn(2+) is ion-exchanged into zeolite-ZSM-5 microstructures, and further coated with poly(diallyldimethylammonium chloride) (PDDA). Direct electron transfer of Mn(2+) is greatly facilitated by zeolite microstructures with the formal potential of 561 ± 6 mV vs. Ag|AgCl, which is just located between thermodynamic potentials of O(2)˙(-)/O(2) and O(2)˙(-)/H(2)O(2). The biomimetic catalytic activity of Mn(3)(PO(4))(2), together with the enhanced electron transfer of Mn(2+) obtained at the zeolite electrode has provided a platform for determination of O(2)˙(-) with high selectivity, wide linear range, low detection limit, and quick response. On the other hand, the present Mn(2+)-ZSM/PDDA electrode shows relatively long-term stability, good reproducibility, and biocompatibility, which opens up a way to adhering cells directly onto the film surface for in situ monitoring of cellular species. As a sequence, the remarkable analytical performance of the present O(2)˙(-) biosensor, combined with the characteristics of the Mn(2+)-ZSM/PDDA electrode surface has established a novel approach for real-time determination of O(2)˙(-) released from living cells.
authors.affiliation: Department of Chemistry, Tongji University, Siping Rode 1239, Shanghai, 200092, China.
authors.forename: Jinqing
authors.initials: J
authors.lastname: Zhou
authors.forename: Yongping
authors.initials: Y
authors.lastname: Luo
authors.forename: Anwei
authors.initials: A
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004579:Electron Transport; D006801:Humans; D008345:Manganese / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000032:analysis*; D017641:Zeolites / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D013481:Superoxides; D017641:Zeolites; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D008345:Manganese; D013482:Superoxide Dismutase