title: Urokinase plasminogen activator receptor in adipose tissue macrophages of morbidly obese subjects.
abstract: OBJECTIVE
  At present, circulating markers characterizing the inflammatory infiltration of white adipose tissue (WAT) in human obesity are not well known. We previously identified, by a pangenomic approach (microarrays), the urokinase plasminogen activator receptor (PLAUR or CD87) as a potential marker of subcutaneous adipose tissue macrophage infiltration (ATM).
  
  
  METHODS
  We studied i) the presence of PLAUR protein in WAT; ii) the PLAUR amount in plasma of obese patients; iii) the circulating variations during surgery-induced weight loss, and iv) the correlations between PLAUR circulating levels and bioclinical parameters.
  
  
  RESULTS
  We observed that PLAUR is preferentially expressed by infiltrating ATMs, with a typical localization on macrophage membrane. Circulating soluble PLAUR levels were significantly elevated in obese patients compared to lean controls. However, despite a trend towards a decrease 3 months after weight loss, PLAUR plasma levels were not modulated during a 1-year weight loss follow-up, suggesting the contribution of secretion sites other than subcutaneous WAT in obese patients.
  
  
  CONCLUSIONS
  These findings indicate that PLAUR mRNA expression could be used for the estimation of local subcutaneous ATMs infiltration in obese patients, but it cannot be used as a systemic marker of this inflammatory infiltration in dynamic phases of weight loss.
authors.affiliation: Inserm U872, CRC Université Pierre et Marie Curie-Paris 6, CHRU Pitié-Salpétrière, Service de Nutrition, Paris, France. r.cancello@auxologico.it
authors.forename: Raffaella
authors.initials: R
authors.lastname: Cancello
authors.forename: Christine
authors.initials: C
authors.lastname: Rouault
authors.forename: Gaël
authors.initials: G
authors.lastname: Guilhem
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bedel
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Di Blasio
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Joan
authors.initials: J
authors.lastname: Tordjman
authors.forename: Karine
authors.initials: K
authors.lastname: Clément
mesh_terms: D052436:Adipose Tissue, White / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D009767:Obesity, Morbid / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D055293:Receptors, Urokinase Plasminogen Activator