title: [Experiment studies on growth and proliferation of Hep-2 cells by silence of TRF2 gene].
abstract: OBJECTIVE
  To investigate the effect of RNA interference silencing telomere repeat factor 2 by observing Hep-2 cells' proliferation and apoptosis in larynx carcinoma cell line.
  
  
  METHODS
  A recombinant plasmid containing a single shRNA (shTRF2) was constructed. The expression of TRF2 gene was detected by PCR and cell proliferation was examined using CCK-8. Hep-2 cells' apoptosis was detected by flow cytometer (FCM).
  
  
  RESULTS
  After treatment with shTRF2, the expression of TRF2 was distinctively depressed, and Hep-2 cells proliferation was obviously inhibited. Compared with control and negative group, cells with treatment of RNAi exhibited significantly more apoptosis.
  
  
  CONCLUSIONS
  Using RNA interference technique to silence TRF2 gene is effective on inhibiting cancer cells' proliferation and help to induce cancer cells' apoptosis.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
authors.forename: Luyan
authors.initials: L
authors.lastname: Jiao
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Zezhang
authors.initials: Z
authors.lastname: Tao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D035341:Telomeric Repeat Binding Protein 2 / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C504753:TERF2 protein, human; D035341:Telomeric Repeat Binding Protein 2