title: Assessment of the potential of a boron-fructose additive in counteracting the toxic effect of Fusarium mycotoxins.
abstract: Trichotecenes are mycotoxins produced by Fusarium sp., which may contaminate animal feeds and human food. A feeding trial was conducted to evaluate the effect of a fusarotoxin-contaminated diet, and to explore the counteracting potential of a calcium fructoborate (CFrB) additive on performance, typical health biochemistry parameters and immune response in weaned pigs. A naturally contaminated maize, containing low doses of deoxynivalenol, zearalenone, fumonisins and T-2/HT-2 toxins (1790, 20, 0·6 and 90 parts per billion), was included in a maize-soyabean meal diet, and given ad libitum to eight weaned piglets (two groups: four pigs/group) for a period of 24 d. CFrB was administered to one of the contaminated groups and to another four piglets as a daily supplement, following the manufacturer's recommendation. A decrease in performance was observed in contaminated animals at this concentration of feed toxins, which was ameliorated by the dietary CFrB supplementation. Fusarium toxins also altered the pig immune response by increasing (P < 0·05) the ex vivo peripheral blood mononuclear cell proliferation (111·7 % in comparison with control), the respiratory burst of porcine granulocytes (15·4 % for responsive cells v. 5·1 % for unstimulated cells and 70·95 v. 22·65 % for stimulated cells, respectively), the percentage of peripheral T, CD3(+), CD3(+)CD4(+) and CD3(+)CD8(+) subsets and the synthesis of IL-1β, TNF-α and IL-8 (123·8, 217·1 and 255·1 %, respectively). The diet containing the CFrB additive reduced these exacerbated cellular immune responses induced by Fusarium toxins. However, consumption of CFrB did not counteract the effect of mycotoxins on biochemistry parameters, and increased plasma IgM and IgG of contaminated pigs.
authors.affiliation: INCDBNA, National Institute of Research and Development for Biology and Animal Nutrition, 1, Calea Bucuresti, 077015 Balotesti, Romania. ionelia.taranu@ibna.ro
authors.forename: Ionelia
authors.initials: I
authors.lastname: Taranu
authors.forename: Daniela E
authors.initials: DE
authors.lastname: Marin
authors.forename: Gina
authors.initials: G
authors.lastname: Manda
authors.forename: Monica
authors.initials: M
authors.lastname: Motiu
authors.forename: Ionela
authors.initials: I
authors.lastname: Neagoe
authors.forename: Cristina
authors.initials: C
authors.lastname: Tabuc
authors.forename: Mariana
authors.initials: M
authors.lastname: Stancu
authors.forename: Margareta
authors.initials: M
authors.lastname: Olteanu
mesh_terms: D000821:Animal Feed; D000818:Animals; D000931:Antidotes / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D001881:Borates / Q000494:pharmacology*; D001895:Boron / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D019587:Dietary Supplements; D005503:Food Additives; D005506:Food Contamination; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D005670:Fusarium; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D007109:Immunity / Q000187:drug effects* / Q000502:physiology; D007136:Immunoglobulins / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D013025:Glycine max; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000378:metabolism; D014255:Trichothecenes / Q000009:adverse effects*; D003313:Zea mays
chemical_list: D000931:Antidotes; D001881:Borates; D016207:Cytokines; D005503:Food Additives; D007136:Immunoglobulins; D014255:Trichothecenes; C507177:calcium fructoborate; D005632:Fructose; D001895:Boron