title: Effect of laser on TNF-alpha expression in inflamed human gingival tissue.
abstract: This study sought to evaluate the effect of low-level laser treatment combined with scaling and root planing (SRP) on gingival tissue levels of TNF-alpha in subjects with periodontal disease. Eighty gingival papilla biopsy samples were obtained from 60 patients diagnosed with chronic advanced periodontitis; randomly assigned to three treatment groups (n = 20), as well as 20 subjects with no periodontal disease (group A). Group B received SRP on a single quadrant/day for four consecutive days. On day 5, all quadrants were rescaled. Groups C and D received the same treatment as group B plus laser application with the low-level diode laser (630-670 nm, 1.875 J/cm(2)) for five and ten consecutive days, respectively. Papilla biopsies were obtained from subjects and evaluated by ELISA for levels of TNF-alpha. The values in the control group were 5.2 ± 3.21 pg/mg and baseline values for the examined groups were 46.01 ± 16.69. Significantly decreased level of TNF-alpha for groups C and D was found after treatment, while group B demonstrated reduction of TNF-alpha of 31.34%. The results of this study show suppression of TNF-alpha in gingival tissue after low-level laser treatment as adjunct to SRP. Data may suggest beneficial anti-inflammatory effects of the laser treatment when used as adjunctive periodontal treatment.
authors.affiliation: Faculty of Dentistry, University Sts Cyril and Methodius, Vodnjanska 17, 1000, Skopje, Macedonia.
authors.forename: Snezana
authors.initials: S
authors.lastname: Pesevska
authors.forename: Marija
authors.initials: M
authors.lastname: Nakova
authors.forename: Icko
authors.initials: I
authors.lastname: Gjorgoski
authors.forename: Nikola
authors.initials: N
authors.lastname: Angelov
authors.forename: Kiro
authors.initials: K
authors.lastname: Ivanovski
authors.forename: Salvador
authors.initials: S
authors.lastname: Nares
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Andreana
mesh_terms: D000328:Adult; D000368:Aged; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000628:therapy; D003131:Combined Modality Therapy; D012534:Dental Scaling; D005260:Female; D005881:Gingiva / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods; D008297:Male; D008875:Middle Aged; D016745:Root Planing; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha