title: The effects of dietary fish oil on inflammation, fibrosis and oxidative stress associated with obstructive renal injury in rats.
abstract: METHODS
  We examined whether dietary supplementation with fish oil modulates inflammation, fibrosis and oxidative stress following obstructive renal injury.
  
  
  RESULTS
  Three groups of Sprague-Dawley rats (n=16 per group) were fed for 4 wk on normal rat chow (oleic acid), chow containing fish oil (33 g eicosapentaenoic acid and 26 g docosahexaenoic acid per kg diet), or chow containing safflower oil (60 g linoleic acid per kg diet). All diets contained 7% fat. After 4 wk, the rats were further subdivided into four smaller groups (n=4 per group). Unilateral ureteral obstruction was induced in three groups (for 4, 7 and 14 days). The fourth group for each diet did not undergo surgery, and was sacrificed as controls at 14 days. When rats were sacrificed, plasma and portions of the kidneys were removed and frozen; other portions of kidney tissue were fixed and prepared for histology. Compared with normal chow and safflower oil, fish oil attenuated collagen deposition, macrophage infiltration, TGF-β expression, apoptosis, and tissue levels of arachidonic acid, MIP-1α, IL-1β, MCP-1 and leukotriene B(4). Compared with normal chow, fish oil increased the expression of HO-1 protein in kidney tissue.
  
  
  CONCLUSIONS
  Fish oil intake reduced inflammation, fibrosis and oxidative stress following obstructive renal injury.
authors.affiliation: The University of Queensland, School of Human Movement Studies, Brisbane, Australia. jpeake@hms.uq.edu.au
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Peake
authors.forename: Glenda C
authors.initials: GC
authors.lastname: Gobe
authors.forename: Robert G
authors.initials: RG
authors.lastname: Fassett
authors.forename: Jeff S
authors.initials: JS
authors.lastname: Coombes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis; D016718:Arachidonic Acid / Q000032:analysis; D018932:Chemokine CCL2 / Q000032:analysis; D054405:Chemokine CCL3 / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D019587:Dietary Supplements; D005355:Fibrosis / Q000188:drug therapy; D005395:Fish Oils / Q000008:administration & dosage* / Q000737:chemistry; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000032:analysis; D007674:Kidney Diseases / Q000188:drug therapy*; D007975:Leukotriene B4 / Q000032:analysis; D008264:Macrophages / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012450:Safflower Oil / Q000008:administration & dosage / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D053583:Interleukin-1beta; D016212:Transforming Growth Factor beta; D007975:Leukotriene B4; D016718:Arachidonic Acid; D012450:Safflower Oil; D003094:Collagen; D051547:Heme Oxygenase-1