title: Process challenges relating to hematopoietic stem cell cultivation in bioreactors.
abstract: Hematopoietic stem cells (HSCs) are extremely useful in treating a wide range of diseases and have a variety of useful research applications. However, the routinely generated low in vitro concentrations of HSCs from current bioreactor manufacturing systems has been a hindrance to the full-scale application of these essential cellular materials. This has made the search for novel bioreactor systems for high-concentration HSC production a major research endeavour. This review addresses process challenges in relation to bioreactor development and optimisation for high-density HSC production under effective monitoring of essential culture parameters, such as pH, dissolved oxygen and nutrient uptake. It discusses different process strategies and bioreactor configurations for HSCs production from a commercial viability perspective, and also discusses recent advances in the field.
authors.affiliation: Bio Engineering Laboratory, Department of Chemical Engineering, Monash University, Wellington Road, Clayton, VIC, 3800, Australia.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kowalczyk
authors.forename: Kathryn
authors.initials: K
authors.lastname: Waldron
authors.forename: Penia
authors.initials: P
authors.lastname: Kresnowati
authors.forename: Michael K
authors.initials: MK
authors.lastname: Danquah
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology*
chemical_list: D003470:Culture Media