title: Reversible heat-induced dissociation of β2-microglobulin amyloid fibrils.
abstract: Recent progress in the field of amyloid research indicates that the classical view of amyloid fibrils, being irreversibly formed highly stable structures resistant to perturbating conditions and proteolytic digestion, is getting more complex. We studied the thermal stability and heat-induced depolymerization of amyloid fibrils of β(2)-microglobulin (β2m), a protein responsible for dialysis-related amyloidosis. We found that freshly polymerized β2m fibrils at 0.1-0.3 mg/mL concentration completely dissociated to monomers upon 10 min incubation at 99 °C. Fibril depolymerization was followed by thioflavin-T fluorescence and circular dichroism spectroscopy at various temperatures. Dissociation of β2m fibrils was found to be a reversible and dynamic process reaching equilibrium between fibrils and monomers within minutes. Repolymerization experiments revealed that the number of extendable fibril ends increased significantly upon incubation at elevated temperatures suggesting that the mechanism of fibril unfolding involves two distinct processes: (1) dissociation of monomers from the fibril ends and (2) the breakage of fibrils. The breakage of fibrils may be an important in vivo factor multiplying the number of fibril nuclei and thus affecting the onset and progress of disease. We investigated the effects of some additives and different factors on the stability of amyloid fibrils. Sample aging increased the thermal stability of β2m fibril solution. 0.5 mM SDS completely prevented β2m fibrils from dissociation up to the applied highest temperature of 99 °C. The generality of our findings was proved on fibrils of K3 peptide and α-synuclein. Our simple method may also be beneficial for screening and developing amyloid-active compounds for therapeutic purposes.
authors.affiliation: Department of Biochemistry, Institute of Biology, Eötvös Loránd University, Budapest H-1117, Hungary. kardos@elte.hu
authors.forename: József
authors.initials: J
authors.lastname: Kardos
authors.forename: András
authors.initials: A
authors.lastname: Micsonai
authors.forename: Henriett
authors.initials: H
authors.lastname: Pál-Gábor
authors.forename: Éva
authors.initials: É
authors.lastname: Petrik
authors.forename: László
authors.initials: L
authors.lastname: Gráf
authors.forename: János
authors.initials: J
authors.lastname: Kovács
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Hironobu
authors.initials: H
authors.lastname: Naiki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
mesh_terms: D000645:Ammonium Sulfate / Q000494:pharmacology; D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding / Q000187:drug effects; D055503:Protein Multimerization / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D058767:Protein Unfolding / Q000187:drug effects; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D001613:beta 2-Microglobulin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000682:Amyloid; D001613:beta 2-Microglobulin; D012967:Sodium Dodecyl Sulfate; D000645:Ammonium Sulfate