title: Luteolin isolated from the medicinal plant Elsholtzia rugulosa (Labiatae) prevents copper-mediated toxicity in β-amyloid precursor protein Swedish mutation overexpressing SH-SY5Y cells.
abstract: Luteolin, a 3',4',5,7-tetrahydroxyflavone, is a plant flavonoid and pharmacologically active agent that has been isolated from several plant species. In the present study, the effects of luteolin obtained from the medicinal plant Elsholtzia rugulosa and the related mechanisms were examined in an Alzheimer's disease (AD) cell model. In this model, copper was used to exacerbate the neurotoxicity in β-amyloid precursor protein Swedish mutation stably overexpressed SH-SY5Y cells (named "APPsw cells" for short). Based on this model, we demonstrated that luteolin increased cell viability, reduced intracellular ROS generation, enhanced the activity of SOD and reversed mitochondrial membrane potential dissipation. Inhibition of caspase-related apoptosis was consistently involved in the neuroprotection afforded by luteolin. Furthermore, it down-regulated the expression of AβPP and lowered the secretion of Aβ₁₋₄₂. These results indicated that luteolin from the Elsholtzia rugulosa exerted neroprotective effects through mechanisms that decrease AβPP expression, lower Aβ secretion, regulate the redox imbalance, preserve mitochondrial function, and depress the caspase family-related apoptosis.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Fanrui
authors.initials: F
authors.lastname: Meng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.forename: Hailin
authors.initials: H
authors.lastname: Qin
authors.forename: Xi
authors.initials: X
authors.lastname: Lan
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Guanhua
authors.initials: G
authors.lastname: Du
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D045744:Cell Line, Tumor; D003300:Copper / Q000633:toxicity*; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D047311:Luteolin / Q000494:pharmacology*; D009154:Mutation; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D016564:Amyloid beta-Protein Precursor; D003300:Copper; D047311:Luteolin