title: Selective, high conversion of D-glucose to 5-keto-D-gluoconate by Gluconobacter suboxydans.
abstract: Selective, high-yield production of 5-keto-D-gluconate (5KGA) from D-glucose by Gluconobacter was achieved without genetic modification. 5KGA production by Gluconobacter suffers byproduct formation of 2-keto-D-gluconate (2KGA). By controlling the medium pH strictly in a range of pH 3.5-4.0, 5KGA was accumulated with 87% conversion yield from D-glucose. The pH dependency of 5KGA formation appeared to be related to that of gluconate oxidizing activity.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ano
authors.forename: Emiko
authors.initials: E
authors.lastname: Shinagawa
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D019149:Bioreactors; D001711:Biotransformation; D005285:Fermentation; D005942:Gluconates / Q000378:metabolism*; D020576:Gluconobacter / Q000201:enzymology; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005942:Gluconates; C008229:2-ketogluconate; C093583:5-ketogluconic acid; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D005947:Glucose; C030691:gluconic acid