title: Isolation, purification, crystallization and preliminary X-ray studies of two 30 kDa proteins from silkworm haemolymph.
abstract: Juvenile hormone-binding protein (JHBP) and the low-molecular-mass lipoprotein PBMHP-12 belong to a group of 30 kDa proteins that comprise the major protein component of the haemolymph specific to the fifth-instar larvae stage of the mulberry silkworm Bombyx mori L. Proteins from this group are often essential for the development of the insect. In a project aimed at crystallographic characterization of B. mori JHBP (BmJHBP), it was copurified together with PBMHP-12. Eventually, the two proteins were isolated and crystallized separately. The BmJHBP crystals were orthorhombic (space group C222(1)) and the PBMHP-12 crystals were triclinic. The crystals diffracted X-rays to 2.9 Å (BmJHBP) and 1.3 Å (PBMHP-12) resolution.
authors.affiliation: Center for Biocrystallographic Research, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
authors.forename: Agnieszka J
authors.initials: AJ
authors.lastname: Pietrzyk
authors.forename: Anna
authors.initials: A
authors.lastname: Bujacz
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Łochyńska
authors.forename: Mariusz
authors.initials: M
authors.lastname: Jaskólski
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bujacz
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006458:Hemolymph / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D007814:Larva / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014961:X-Ray Diffraction
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; D008074:Lipoproteins; C035606:juvenile hormone-binding protein, insect