title: A review molecular typing methods for Aspergillus flavus isolates.
abstract: Aspergillus flavus is the second most important Aspergillus species causing human infections. The importance of this fungus increases in regions with a dry and hot climate. Small phylogenetic studies in Aspergillus flavus indicate that the morphological species contains several genetically isolated species. Different genotyping methods have been developed and employed in order to better understand the genetic and epidemiological relationships between environmental and clinical isolates. Understanding pathogen distribution and relatedness is essential for determining the epidemiology of nosocomial infections and aiding in the design of rational pathogen control methods. Typing techniques can also give us a deeper understanding of the colonization pattern in patients. Most of these studies focused on Aspergillus fumigatus because it is medically the most isolated species. To date, there has not been any publication exclusively reviewing the molecular typing techniques for Aspergillus flavus in the literature. This article reviews all these different available methods for this organism.
authors.affiliation: Faculté de médecine, Laboratoire de biologie moléculaire parasitaire et fongique, Sfax, Tunisie. ineshadrich@yahoo.fr
authors.forename: Inès
authors.initials: I
authors.lastname: Hadrich
authors.forename: Fattouma
authors.initials: F
authors.lastname: Makni
authors.forename: Sourour
authors.initials: S
authors.lastname: Neji
authors.forename: Fatma
authors.initials: F
authors.lastname: Cheikhrouhou
authors.forename: Hayet
authors.initials: H
authors.lastname: Sellami
authors.forename: Ali
authors.initials: A
authors.lastname: Ayadi
mesh_terms: D001231:Aspergillus flavus / Q000145:classification* / Q000235:genetics*; D001232:Aspergillus fumigatus / Q000145:classification / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length