title: Effects of co-administration of dopamine and vitamin C on ischaemia-reperfusion injury after experimental testicular torsion-detorsion in rats.
abstract: The objective of this study was to investigate the effects of dopamine as vasodilator, vitamin C as an antioxidant and combined administration of them on ischaemia-reperfusion (I-R) injury following testicular torsion (TT). Thirty adult male rats were divided into six groups each containing five rats. Testicular ischaemia was achieved by twisting the left testis for 4 h. Group 1 was for determination of the basal values. Group 2 had 4 h TT. Group 3 had 4 h TT and was then treated with dopamine. Group 4 had 4 h TT and was then treated with vitamin C. Group 5 had 4 h TT and was then treated with dopamine and vitamin C. Group 6 was designed as a sham operated group. Testicular torsion caused a significant decrease in the percentage of spermatogenesis and seminiferous tubules diameters compared with the control and sham groups. Administration of dopamine, vitamin C and their combination increased above mentioned parameters and decreased serum malondialehyde levels significantly. However, vitamin C had better results than the other treatments (P < 0.05). In conclusion, a potent antioxidant like vitamin C was found to be more effective than increasing blood flow by a vasodilator like dopamine on improving I-R injury following TT.
authors.affiliation: Department of Clinical Sciences, Shahid Bahonar University of Kerman, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Azizollahi
authors.forename: H
authors.initials: H
authors.lastname: Babaei
authors.forename: A
authors.initials: A
authors.lastname: Derakhshanfar
authors.forename: M M
authors.initials: MM
authors.lastname: Oloumi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage*; D004298:Dopamine / Q000008:administration & dosage*; D004359:Drug Therapy, Combination; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000209:etiology; D012671:Seminiferous Tubules / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000150:complications* / Q000473:pathology; D013091:Spermatogenesis; D014665:Vasodilator Agents / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D014665:Vasodilator Agents; D008315:Malondialdehyde; D001205:Ascorbic Acid; D004298:Dopamine