title: Rapid deletion-based subtyping system for the Manila family of Mycobacterium tuberculosis.
abstract: The Manila family of Mycobacterium tuberculosis is a group of clonal isolates seen throughout the Pacific Basin. Commonly used rapid molecular typing methods often leave large groups of Manila family isolates clustered together. Here we describe a simple deletion-based PCR method that improves the discrimination for Manila family isolates, with or without the use of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) classification, and that is both rapid and affordable. Twenty-eight Manila family isolates, classified by spoligotyping, were collected from around the Pacific Basin from 1995 to 2003 and were tested for known genomic deletions. Nine of 15 regions of difference tested were identified as potentially discriminatory, with 18 distinct patterns; of these 9, 5 were selected for optimal discrimination using 61 Manila family isolates collected from California in 2009. For this geographically limited sample, the single large cluster was reduced to 14 distinct patterns. When the isolates were tested by spoligotyping and MIRU-VNTR, the addition of deletion analysis increased the number of distinct patterns from 43 to 56. In summary, the two study groups, which together form a single group of 89 isolates by spoligotyping, were segregated into 17 subgroups by our deletion-based subtyping system.
authors.affiliation: Department of Microbiology, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Frink
authors.forename: Lishi
authors.initials: L
authors.lastname: Qian
authors.forename: Steven
authors.initials: S
authors.lastname: Yu
authors.forename: Laura
authors.initials: L
authors.lastname: Cruz
authors.forename: Ed
authors.initials: E
authors.lastname: Desmond
authors.forename: James T
authors.initials: JT
authors.lastname: Douglas
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002140:California; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D017384:Sequence Deletion