title: Single-run analysis of isomers of retinoyl-beta-D-glucuronide and retinoic acid by reversed-phase high-performance liquid chromatography.
abstract: Reversed-phase HPLC methods capable of separating several retinoic acid isomers are generally not designed for simultaneous analysis of isomers of other classes of retinoids. A reversed-phase HPLC method is presented which allows the separation of at least four retinoic acid (RA) isomers (13-cis-RA, 9,13-dicis-RA, 9-cis-RA, all-trans-RA)and of all isomers of retinoyl-beta-D-glucuronide (RAG), which have been observed in vivo (13-cis-RAG, 9-cis-RAG,all-trans-RAG). The recovery of retinoids was generally between 80 and 90%. Intra-day reproducibility (expressed as relative standard deviation) was less than or equal to 7%. As little as 0.25 ng of RA isomers and of all-trans-RAG could be detected. This method allowed the study of the metabolism of 9-cis-retinoids, where isomerization reactions play a predominant role.
authors.affiliation: lnstitut fiir Toxikologie und Emhr~opharmakologie, Freie Umversiriit Berlin. Garystrasse 5, D-14195 Berlin, Germany.
authors.forename: J O
authors.initials: JO
authors.lastname: Sass
authors.forename: H
authors.initials: H
authors.lastname: Nau
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005260:Female; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D014212:Tretinoin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry* / Q000378:metabolism
chemical_list: C026787:retinoyl glucuronide; D014212:Tretinoin