title: Comparison of speedy PCR-ssp method and serological typing of HLA-A24 for Japanese cancer patients.
abstract: Human leukocyte antigen (HLA) typing is essential to carry out HLA-class I restricted antigenic peptide-based cancer immunotherapy. To establish a one-step polymerase chain reaction-sequence-specific primer (PCR-SSP) method, we designed two novel HLA-A24-specific primer sets and determined the optimal conditions for specific amplification. Then, we performed HLA-A24 typing of two healthy donors' and 17 cancer patients' peripheral blood with serological typing and PCR-SSP typing. Eleven of the 19 cases were determined HLA-A24-positive by the PCR-SSP method precisely; however, five cases showed false positive with serological analysis. Thus, for HLA-A24 typing in the Japanese population, the PCR-SSP method is faster and more accurate than serological typing.
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Munehide
authors.initials: M
authors.lastname: Nakatsugawa
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Hirohashi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Satoko
authors.initials: S
authors.lastname: Inoda
authors.forename: Kenji
authors.initials: K
authors.lastname: Kiriyama
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Eiji
authors.initials: E
authors.lastname: Sato
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D005434:Flow Cytometry; D015234:HLA-A Antigens / Q000235:genetics*; D059871:HLA-A24 Antigen; D006801:Humans; D007564:Japan; D008297:Male; D009369:Neoplasms / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D015234:HLA-A Antigens; D059871:HLA-A24 Antigen