title: Cryopreservation of transgenic sheep lines.
abstract: Although a method exists for freezing sheep embryos directly into liquid nitrogen without a programmable freezing machine, a more traditional slow cooling method is described here. Either dimethyl sulfoxide (DMSO) or ethylene glycol can be used as a cryoprotectant in this slow cooling method. Pregnancy rates for frozen embryos are similar to those of fresh embryos. For a general discussion of cryopreservation, the reader is referred to Chapter 25.
authors.affiliation: Women's Healthcare Specialists. Mount Sinai Medical Office Pavilion, Miami Beach, FL.
authors.forename: K O
authors.initials: KO
authors.lastname: Pomeroy
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D012756:Sheep / Q000235:genetics*