title: Cytotoxicity assessment of monocrotophos in Paramecium caudatum and Oxytricha fallax.
abstract: Experiments were conducted to evaluate the toxic effects ofmonocrotophos in ciliate models Paramecium caudatum and Oxytricha fallax. In acute toxicity studies higherconcentrations of monocrotophos caused marked increase in mobility of cells exhibiting rocking movements within two mins of exposure but were decreased after 30 mins. LC50 value by mortality curve for 3 hr acute toxicity test of Oxytricha fallax and Paramecium caudatum was found 307.744 +/- 33.27 mg l(-1) and 332.284 +/- 57.52 mg l(-1) respectively. Oxytricha fallax was found sensitive than Paramecium caudatum to monocrotophos. In acute exposure cells showed deformities such as swelling, oval shaped deformity and in higher concentrations shortening of longitudinal axis with blackening of cytoplasm occurred. The length of paramecia was reduced prominently. Similarly enlargement of contractile vacuole and stress egestion of food vacuoles was also observed. The morphological studies showed the changes in shape, size, colour and width of Paramecia and Oxytricha. Frequencies of macronuclear aberrations were significant showing deformities such as rod shaped, elongation, fragmentation, diffusion and total absence of nucleus and were concentration dependent. The data provided in the present study on interaction of pesticides with nuclear structure can be of immense value because most of these pesticides have been reported to have carcinogenic, mutagenic and teratogenic properties.
authors.affiliation: Protozoology and Environmental Toxicology Lab., Department of Zoology, Osmania University, Hyderabad - 500 007, India. nageswar_ou@yahoo.co.in
authors.forename: Nageswara Rao
authors.initials: NR
authors.lastname: Amanchi
authors.forename: Mohd Masood
authors.initials: MM
authors.lastname: Hussain
mesh_terms: D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D008999:Monocrotophos / Q000008:administration & dosage / Q000633:toxicity*; D016813:Oxytricha / Q000187:drug effects*; D048488:Paramecium caudatum / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D007306:Insecticides; D014874:Water Pollutants, Chemical; D008999:Monocrotophos