title: The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold.
abstract: The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
authors.forename: Chul Won
authors.initials: CW
authors.lastname: Lee
authors.forename: Lichun
authors.initials: L
authors.lastname: Li
authors.forename: David P
authors.initials: DP
authors.lastname: Giedroc
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D020029:Base Pairing; D001483:Base Sequence; D017124:Conserved Sequence; D055029:Inverted Repeat Sequences / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics*; D012996:Solutions; D013329:Structure-Activity Relationship
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D012996:Solutions