title: MiR-203 controls proliferation, migration and invasive potential of prostate cancer cell lines.
abstract: Prostate cancers show a slow progression from a local lesion (primary tumor) to a metastatic and hormone-resistant phenotype. After an initial step of hyperplasia, in a high percentage of cases a neoplastic transformation event occurs that, less frequently, is followed by epithelial to mesenchymal transition and invasion of healthy tissues (usually bones). MicroRNA-203 (miR-203) is a tumor suppressor microRNA often silenced in different malignancies. Here, we show that miR-203 is downregulated in clinical primary prostatic tumors compared to normal prostate tissue, and in metastatic prostate cancer cell lines compared to normal epithelial prostatic cells. Overexpression of miR-203 in brain or bone metastatic prostate cell lines (DU145 and PC3) is sufficient to induce a mesenchymal to epithelial transition with inhibition of cell proliferation, migration and invasiveness. We have identified CKAP2, LASP1, BIRC5, WASF1, ASAP1 and RUNX2 as new miR-203 direct target mRNAs involved in these events. Therefore, miR-203 could be a potentially new prognostic marker and therapeutic target in metastatic prostate cancer.
authors.affiliation: Department of experimental Medicine and Biochemical Sciences and Biochemistry Laboratory IDI-IRCCS, University of Rome Tor Vergata, Rome, Italy.
authors.forename: Giuditta
authors.initials: G
authors.lastname: Viticchiè
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Lena
authors.forename: Alessia
authors.initials: A
authors.lastname: Latina
authors.forename: Amanda
authors.initials: A
authors.lastname: Formosa
authors.forename: Lea H
authors.initials: LH
authors.lastname: Gregersen
authors.forename: Anders H
authors.initials: AH
authors.lastname: Lund
authors.forename: Sergio
authors.initials: S
authors.lastname: Bernardini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mauriello
authors.forename: Roberto
authors.initials: R
authors.lastname: Miano
authors.forename: Luigi G
authors.initials: LG
authors.lastname: Spagnoli
authors.forename: Richard A
authors.initials: RA
authors.lastname: Knight
authors.forename: Eleonora
authors.initials: E
authors.lastname: Candi
authors.forename: Gerry
authors.initials: G
authors.lastname: Melino
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D046228:Microarray Analysis; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology*; D011471:Prostatic Neoplasms / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D017931:DNA Primers; C546703:MIRN203 microRNA, human; D035683:MicroRNAs