title: Metabolism of soluble sugars in developing melon fruit: a global transcriptional view of the metabolic transition to sucrose accumulation.
abstract: The sweet melon fruit is characterized by a metabolic transition during its development that leads to extensive accumulation of the disaccharide sucrose in the mature fruit. While the biochemistry of the sugar metabolism pathway of the cucurbits has been well studied, a comprehensive analysis of the pathway at the transcriptional level allows for a global genomic view of sugar metabolism during fruit sink development. We identified 42 genes encoding the enzymatic reactions of the sugar metabolism pathway in melon. The expression pattern of the 42 genes during fruit development of the sweet melon cv Dulce was determined from a deep sequencing analysis performed by 454 pyrosequencing technology, comprising over 350,000 transcripts from four stages of developing melon fruit flesh, allowing for digital expression of the complete metabolic pathway. The results shed light on the transcriptional control of sugar metabolism in the developing sweet melon fruit, particularly the metabolic transition to sucrose accumulation, and point to a concerted metabolic transition that occurs during fruit development.
authors.affiliation: Institute of Plant Science, Agricultural Research Organization, Bet Dagan, Israel.
authors.forename: Nir
authors.initials: N
authors.lastname: Dai
authors.forename: Shahar
authors.initials: S
authors.lastname: Cohen
authors.forename: Vitaly
authors.initials: V
authors.lastname: Portnoy
authors.forename: Galil
authors.initials: G
authors.lastname: Tzuri
authors.forename: Rotem
authors.initials: R
authors.lastname: Harel-Beja
authors.forename: Maya
authors.initials: M
authors.lastname: Pompan-Lotan
authors.forename: Nir
authors.initials: N
authors.lastname: Carmi
authors.forename: Genfa
authors.initials: G
authors.lastname: Zhang
authors.forename: Alex
authors.initials: A
authors.lastname: Diber
authors.forename: Sarah
authors.initials: S
authors.lastname: Pollock
authors.forename: Hagai
authors.initials: H
authors.lastname: Karchi
authors.forename: Yelena
authors.initials: Y
authors.lastname: Yeselson
authors.forename: Marina
authors.initials: M
authors.lastname: Petreikov
authors.forename: Shmuel
authors.initials: S
authors.lastname: Shen
authors.forename: Uzi
authors.initials: U
authors.lastname: Sahar
authors.forename: Ran
authors.initials: R
authors.lastname: Hovav
authors.forename: Efraim
authors.initials: E
authors.lastname: Lewinsohn
authors.forename: Yakov
authors.initials: Y
authors.lastname: Tadmor
authors.forename: David
authors.initials: D
authors.lastname: Granot
authors.forename: Ron
authors.initials: R
authors.lastname: Ophir
authors.forename: Amir
authors.initials: A
authors.lastname: Sherman
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.forename: Jim
authors.initials: J
authors.lastname: Giovannoni
authors.forename: Yosef
authors.initials: Y
authors.lastname: Burger
authors.forename: Nurit
authors.initials: N
authors.lastname: Katzir
authors.forename: Arthur A
authors.initials: AA
authors.lastname: Schaffer
mesh_terms: D016000:Cluster Analysis; D028463:Cucumis melo / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D004798:Enzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012995:Solubility; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004798:Enzymes; D010940:Plant Proteins; D013395:Sucrose