title: [Endothelial genesis inhibitor-8t (EDI-8t) against tumor growth].
abstract: On the basis of the origin comparison of known endothelial genesis inhibitors, a 417-bp cDNA fragment was amplified from umbilical cord by RT-PCR and cloned into the expression vector pPIC9, followed by transformation into Pichia pastoris GS115. The resulted yeast was induced with methanol to express recombinant protein. The resulted protein was purified from culture broth and designated as EDI-8t. The in vitro study showed that EDI-8t, originated from collagen VIII, could specifically inhibit the growth and migration of bovine aortic endothelial cells (BAEC) stimulated by basic fibroblast growth factor (bFGF). The protein also exhibited the activity to cause cell apoptosis. In vivo EDI-8t showed the identical activity comparing with endostatin to inhibit the growth of liver tumor transplanted into nude mice. Interestingly, EDI-8t showed higher activity than endostatin to inhibit tumor growth in metastatic model of melanoma mice.
authors.affiliation: Shanghai Apeloa Pharmaceutical Research Institute, Shanghai 201314, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Zhou
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Yue
authors.initials: Y
authors.lastname: Qian
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Baoshan
authors.initials: B
authors.lastname: Feng
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Ding
authors.forename: Renbao
authors.initials: R
authors.lastname: Gan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D020533:Angiogenesis Inhibitors / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D024102:Collagen Type VIII / Q000737:chemistry* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells / Q000737:chemistry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D024102:Collagen Type VIII; D011994:Recombinant Proteins