title: An effect of DNA sequence on nucleosome occupancy and removal.
abstract: A barrier phases nucleosomes at the yeast (Saccharomyces cerevisiae) GAL1-GAL10 genes. Here we separate nucleosome positioning from occupancy and show that the degree of occupancy of these phased sites is predictably determined by the underlying DNA sequences. As this occupancy is increased (by sequence alteration), nucleosome removal upon induction is decreased, as is mRNA production. These results explain why promoter sequences have evolved to form nucleosomes relatively inefficiently.
authors.affiliation: Molecular Biology Program, Sloan-Kettering Institute, New York, New York, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Gene O
authors.initials: GO
authors.lastname: Bryant
authors.forename: Monique
authors.initials: M
authors.lastname: Floer
authors.forename: Dan
authors.initials: D
authors.lastname: Spagna
authors.forename: Mark
authors.initials: M
authors.lastname: Ptashne
mesh_terms: D009707:Nucleosomes / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D009707:Nucleosomes