title: Implementation of a de novo genome-wide computational approach for updating Brachypodium miRNAs.
abstract: Plant microRNAs (miRNAs) are single-stranded 20-22 nt small RNAs (sRNA) that are produced from their own genes. We have developed a de novo genome-wide approach for the computational identification of novel plant miRNAs based on the integration of the complete genome sequence with sRNA libraries. It comprises three modules - the clustering module identifies genomic regions that have two closely-located unidirectional sRNA clusters, the mirplan module explores the secondary structure of the genomic regions, and the duplex module predicts miRNA/miRNA* duplexes. We applied our approach to the Brachypodium genome and publicly available sRNA libraries and predicted 102 miRNAs. Our results extend the list of known miRNAs with 58 novel miRNAs and define the genomic loci of all predicted miRNAs. Because this approach considers specific features of plant miRNAs, it can be employed for the analysis of the genome and sRNA libraries generated for plant species to achieve systematic miRNA discovery.
authors.affiliation: Department of Plant Physiology and Molecular Biology, University of Plovdiv, 24 Tsar Assen St, 4000 Plovdiv, Bulgaria. vebaev@gmail.com
authors.forename: Vesselin
authors.initials: V
authors.lastname: Baev
authors.forename: Ivan
authors.initials: I
authors.lastname: Milev
authors.forename: Mladen
authors.initials: M
authors.lastname: Naydenov
authors.forename: Elena
authors.initials: E
authors.lastname: Apostolova
authors.forename: Georgi
authors.initials: G
authors.lastname: Minkov
authors.forename: Ivan
authors.initials: I
authors.lastname: Minkov
authors.forename: Galina
authors.initials: G
authors.lastname: Yahubyan
mesh_terms: D001483:Base Sequence; D058431:Brachypodium / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D015723:Gene Library; D018745:Genome, Plant / Q000235:genetics*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D035683:MicroRNAs