title: The Drosophila L1CAM homolog Neuroglian signals through distinct pathways to control different aspects of mushroom body axon development.
abstract: The spatiotemporal integration of adhesion and signaling during neuritogenesis is an important prerequisite for the establishment of neuronal networks in the developing brain. In this study, we describe the role of the L1-type CAM Neuroglian protein (NRG) in different steps of Drosophila mushroom body (MB) neuron axonogenesis. Selective axon bundling in the peduncle requires both the extracellular and the intracellular domain of NRG. We uncover a novel role for the ZO-1 homolog Polychaetoid (PYD) in axon branching and in sister branch outgrowth and guidance downstream of the neuron-specific isoform NRG-180. Furthermore, genetic analyses show that the role of NRG in different aspects of MB axonal development not only involves PYD, but also TRIO, SEMA-1A and RAC1.
authors.affiliation: Laboratory of Developmental Genetics, Department of Human Genetics, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Tim
authors.initials: T
authors.lastname: Goossens
authors.forename: Yuan Y
authors.initials: YY
authors.lastname: Kang
authors.forename: Gunther
authors.initials: G
authors.lastname: Wuytens
authors.forename: Pascale
authors.initials: P
authors.lastname: Zimmermann
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Callaerts-Végh
authors.forename: Giulia
authors.initials: G
authors.lastname: Pollarolo
authors.forename: Rafique
authors.initials: R
authors.lastname: Islam
authors.forename: Michael
authors.initials: M
authors.lastname: Hortsch
authors.forename: Patrick
authors.initials: P
authors.lastname: Callaerts
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D024521:Mushroom Bodies / Q000166:cytology* / Q000378:metabolism*; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics / Q000378:metabolism*; D038081:Organogenesis / Q000235:genetics / Q000502:physiology; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D039842:Neural Cell Adhesion Molecule L1; C061745:Nrg protein, Drosophila