title: [Molecular cloning of Litopenaeus vannamei TCP-1-eta gene and analysis on its relationship with cold tolerance].
abstract: To study the molecular mechanism of cold tolerance, we cloned TCP-1-eta homolog gene from Litopenaeus vannamei and studied its relationship with cold tolerance. Based on the sequence from electronic cloning, a pair of primers were designed, and a 1 705 bp cDNA was obtained by RT-PCR. The cDNA contains 1 629 bp ORF, which encodes a peptide of 542 aa. Tissue expression pattern by real-time PCR analysis revealed that TCP-1-eta mRNA mainly existed in muscle tissue. Cold induction by different temperatures revealed that TCP-1-eta mRNA expression started to increase after treatment at 15°C for 36 h, and the increase was remarkable after treatment at 13°C. As for treatment at 13°C for different times, the expression pattern was almost not changed during the first 36 h, but significantly increased after 36 h of treatment. A SNP (single nucleotide polymorphism) site was found in 731 bp of ORF, and the genotypes of 216 Litopenaeus vannamei were determined by PCR-RFLP. Variance analysis indicated that the SNP genotype was significantly related with cold tolerance parameter Cooling-Degree Hours (CDH), and CDH for CC type of Litopenaeus vannamei was longer than TT type.
authors.affiliation: Guangxi Fishery Institute, Nanning 530021, China. hnyinqin@yahoo.com.cn
authors.forename: Qin
authors.initials: Q
authors.lastname: Yin
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Peng
authors.forename: Liang
authors.initials: L
authors.lastname: Cui
authors.forename: Da-Xiang
authors.initials: DX
authors.lastname: Xie
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Wang
authors.forename: Kui
authors.initials: K
authors.lastname: Li
authors.forename: Xiao-Han
authors.initials: XH
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003080:Cold Temperature; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics / Q000502:physiology*
chemical_list: D018834:Chaperonin 60