title: Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid.
abstract: The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ(6,9,12)) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4 % of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
authors.affiliation: Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wan
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Mulan
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010843:Pichia; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017965:gamma-Linolenic Acid / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D011994:Recombinant Proteins; D017965:gamma-Linolenic Acid; D050602:Linoleoyl-CoA Desaturase