title: Dendrotoxin-κ suppresses tumor growth induced by human lung adenocarcinoma A549 cells in nude mice.
abstract: Voltage-gated K(+) (Kv) channels have been considered to be a regulator of membrane potential and neuronal excitability. Recently, accumulated evidence has indicated that several Kv channel subtypes contribute to the control of cell proliferation in various types of cells and are worth noting as potential emerging molecular targets of cancer therapy. In the present study, we investigated the effects of the Kv1.1-specific blocker, dendrotoxin-κ (DTX-κ, on tumor formation induced by the human lung adenocarcinoma cell line A549 in a xenograft model. Kv1.1 mRNA and protein was expressed in A549 cells and the blockade of Kv1.1 by DTX-κ, reduced tumor formation in nude mice. Furthermore, treatment with DTX-κ significantly increased protein expression of p21(Waf1/Cip1), p27(Kip1), and p15(INK4B) and significantly decreased protein expression of cyclin D3 in tumor tissues compared to the control. These results suggest that DTX-κ has anti-tumor effects in A549 cells through the pathway governing G1-S transition.
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
authors.forename: Soo Hwa
authors.initials: SH
authors.lastname: Jang
authors.forename: Pan Dong
authors.initials: PD
authors.lastname: Ryu
authors.forename: So Yeong
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004546:Elapid Venoms / Q000494:pharmacology*; D017815:Elapidae; D006801:Humans; D051662:Kv1.1 Potassium Channel / Q000037:antagonists & inhibitors* / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D014183:Transplantation, Heterologous
chemical_list: D004546:Elapid Venoms; D026902:Potassium Channel Blockers; D012333:RNA, Messenger; D051662:Kv1.1 Potassium Channel; C020273:dendrotoxin