title: Production of fibrinolytic enzyme from Bacillus amyloliquefaciens by fermentation of chickpeas, with the evaluation of the anticoagulant and antioxidant properties of chickpeas.
abstract: To develop safe and cheap thrombolytic agents, a fibrinolytic enzyme productive strain of LSSE-62 was isolated from Chinese soybean paste. This strain was identified as Bacillus amyloliquefaciens by 16S rDNA sequence analysis. Nucleotide and amino acid sequence analysis showed that this fibrinolytic enzyme was identical to subtilisin DJ-4. Chickpeas were used as the substrate for fibrinolytic enzyme production from B. amyloliquefaciens in solid-state fermentation. Under the optimized conditions (34 °C and 50% initial moisture content), the fibrinolytic activity of fermented chickpeas reached 39.28 fibrin degradation units (FU)/g. Additionally, the fermented chickpeas showed anticoagulant activity, and the purified anticoagulant component showed higher anticoagulant activity than heparin sodium. After fermentation, the total phenolic and total flavonoid contents increased by 222 and 71%, respectively, and then the antioxidant activities were improved significantly. This study provided a novel method for the preparation of multifunctional food of chickpeas or raw materials for the preparation of functional food additives and potential drugs.
authors.affiliation: Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Xuetuan
authors.initials: X
authors.lastname: Wei
authors.forename: Mingfang
authors.initials: M
authors.lastname: Luo
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yewei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Lin
authors.forename: Peng
authors.initials: P
authors.lastname: Kong
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000925:Anticoagulants / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D029863:Cicer; D003001:Cloning, Molecular; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D005285:Fermentation; D005342:Fibrinolysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S