title: Mechanisms of anti-inflammatory effect of an active ingredients group from Jinxuan Zhike Xunxi San.
abstract: OBJECTIVE
  JZXS has been used for inflamed hemorrhoids as a chinese prescription for several decades. The present study was designed to investigate the anti-inflammatory activities, as well as the mechanisms, of an active ingredients group (AIG) obtained from Jinxuan Zhike Xunxi San (JZXS).
  
  
  METHODS
  The anti-inflammatory activities and mechanisms of AIG were evaluated by xylene-induced ear edema experiments in normal mice and mice without adrenals, leukocyte migration experiments, and carrageenin-induced peritonitis experiments, taking JZXS as the positive control.
  
  
  RESULTS
  AIG and JZXS prevented xylene-induced ear edema in normal mice but showed no effects when adrenals were removed. Additionally, AIG and JZXS inhibited leukocyte migration, reduced prostaglandin E2 (PGE2) level in inflammatory exudates and nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 and 8 (IL-6 and IL-8) levels in serum.
  
  
  CONCLUSIONS
  AIG and JZXS showed significant anti-inflammatory activities depending on pituitary-adrenal axis, thereby inhibiting leukocyte migration and reducing cytokines and mediators.
authors.affiliation: Ministry of Education Key Laboratory of Traditional Chinese Medicine Resources and Compounds, Hubei College of Traditional Chinese Madical, Hubei, Wuhan 430061, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wen
authors.forename: Lu
authors.initials: L
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Hegui
authors.initials: H
authors.lastname: Huang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Wenying
authors.initials: W
authors.lastname: Zhang
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D015232:Dinoprostone / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D015232:Dinoprostone