title: Regulation of iodothyronine deiodinases and sodium iodide symporter mRNA expression by perchlorate in larvae and adult Chinese rare minnow (Gobiocypris rarus).
abstract: Perchlorate is a widespread contaminant in the aquatic environment. In the present work, the expressions of deiodinase enzymes (d1, d2, and d3) and sodium iodide symporter (nis) genes were determined after larval and adult rare minnow (Gobiocypris rarus) exposed to 5 and 50 μg/L perchlorate for 21 days. The results showed that deflation of swim bladder development was observed in larvae at 50 μg/L perchlorate treatment. An up-regulation of the d2 and nis mRNA levels were observed in the larve and in brain of adults. Meanwhile the expressions of d3 mRNA levels were significantly down-regulated in the liver. These results indicate the changes in d2, nis, and d3 mRNA expression brings about increased outer-ring deiodination, idodine uptake, and a further decrease of inner-ring deiodination, respectively reflecting auto-regulation of hypothalamic-pituitary-thyroid (HPT) axis in adult after perchlorate exposure. The larval fish development could be affected by perchlorate at environmentally relevant concentrations.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Jinmiao
authors.initials: J
authors.lastname: Zha
authors.forename: Lihua
authors.initials: L
authors.lastname: Yang
authors.forename: Zhaoli
authors.initials: Z
authors.lastname: Li
authors.forename: Zijian
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D003530:Cyprinidae; D052244:Endocrine Disruptors / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects; D007453:Iodide Peroxidase / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010472:Perchlorates / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D013963:Thyroid Hormones / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000099209:Sodium-Iodide Symporters
chemical_list: D052244:Endocrine Disruptors; D007453:Iodide Peroxidase; D010472:Perchlorates; D012333:RNA, Messenger; D027981:Symporters; D013963:Thyroid Hormones; D014874:Water Pollutants, Chemical; D000099209:Sodium-Iodide Symporters; C494474:perchlorate