title: Validation study of the VitaKit A test kit for the determination of vitamin A in fluid milk (2% fat) for routine quality control. Performance Tested Method 061001.
abstract: A validation study of VitaKit A for quantitation of total vitamin A in 2% fluid milk was carried out according to the guidelines provided by AOAC INTERNATIONAL. The VitaKit A was compared, in terms of repeatability and accuracy, with the U.S. Food and Drug Administration-Interstate Milk Shippers HPLC reference method for determination of total vitamin A in fluid milk with 2% fat. The data obtained by the VitaKit A method are in excellent agreement with the data obtained by the HPLC reference method. Further, a low LOD (0.33 international unit/mL) was obtained for the VitaKit A method; the presence of interferents, like cholesterol and vitamin D3, in the milk had minor influence on the quantitation of total vitamin A by the VitaKit A method. The VitaKit A test kit was found to be stable for 1 year from the date of manufacture when stored at 2-8 degrees C. The method requires 2 h processing time, compared to 1-2 days for the HPLC reference method. The results of this validation study clearly demonstrate that the VitaKit A method is reliable, rapid, and accurate for the quantitation of total vitamin A in fluid milk containing 2% milk fat. An independent study by Q Laboratories Inc., Cincinnati, OH, under the validation guidelines of AOAC INTERNATIONAL, confirmed these findings.
authors.affiliation: SciMed Technologies Inc., Advance Technology Center, Suite 209, 9650-20 Ave, Edmonton, Alberta, Canada T6N 1G1. sgupta@scimedtechnologies.com
authors.forename: Seema
authors.initials: S
authors.lastname: Gupta
authors.forename: Xihua
authors.initials: X
authors.lastname: Sui
authors.forename: Robert
authors.initials: R
authors.lastname: Sikora
authors.forename: Kailash
authors.initials: K
authors.lastname: Banasure
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005504:Food Analysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D008892:Milk / Q000737:chemistry* / Q000592:standards; D011786:Quality Control; D015203:Reproducibility of Results; D014801:Vitamin A / Q000032:analysis* / Q000592:standards
chemical_list: D014801:Vitamin A