title: Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation.
abstract: Mest (mesoderm-specific transcript)/Peg1 (paternally expressed gene 1) is an imprinted gene that plays important roles in embryo development, although its biochemical role has not been determined. Ectopic expression of Mest/Peg1 inhibited Wnt-mediated reporter activity by enhancing the ubiquitination of β-catenin. The maturation and plasma membrane localization of the Wnt co-receptor LRP6 [LDLR (low-density lipoprotein receptor)-related protein 6], which are both necessary for Wnt signalling, were blocked by the expression of Mest/Peg1. Mest/Peg1 inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic differentiation.
authors.affiliation: Department of Life Science, The University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Korea.
authors.forename: Hwajin
authors.initials: H
authors.lastname: Jung
authors.forename: Suk Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Eek-hoon
authors.initials: EH
authors.lastname: Jho
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D026502:LDL-Receptor Related Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D051379:Mice; D011506:Proteins / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D051153:Wnt Proteins / Q000037:antagonists & inhibitors* / Q000502:physiology*
chemical_list: D026502:LDL-Receptor Related Proteins; C555475:LRP6 protein, human; D060488:Low Density Lipoprotein Receptor-Related Protein-6; C555476:Lrp6 protein, mouse; D011506:Proteins; D051153:Wnt Proteins; C103637:mesoderm specific transcript protein