title: Surface immobilization of antibody on silk fibroin through conformational transition.
abstract: In recent studies silk fibroin has been explored as a new material platform for biosensors. Based on these developments, a procedure for the immobilization of antibodies on silk fibroin substrates was developed as a route to functionalizing these biosensor systems. By controlling the conformational transition of the silk fibroin, a primary antibody was immobilized and enriched at the surface of silk fibroin substrates under mild reaction conditions to maintain antibody function. Compared to chemical crosslinking, the immobilization efficiency in the present approach was increased significantly. This method, achieving high loading of antibody while retaining function, improves the feasibility of silk fibroin as a platform material for biosensor applications.
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, People's Republic of China. lvqiang78@suda.edu.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.forename: Hesun
authors.initials: H
authors.lastname: Zhu
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D012831:Bombyx / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D008422:Materials Testing; D011487:Protein Conformation; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D005349:Fibroins