title: Identification of new defective interfering RNA species associated with porcine reproductive and respiratory syndrome virus infection.
abstract: In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China.
authors.forename: Chao-Ting
authors.initials: CT
authors.lastname: Xiao
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Xing-Long
authors.initials: XL
authors.lastname: Yu
authors.forename: Meng
authors.initials: M
authors.lastname: Ge
authors.forename: Run-Cheng
authors.initials: RC
authors.lastname: Li
authors.forename: Bo-Ren
authors.initials: BR
authors.lastname: Xiao
authors.forename: Hong-Rui
authors.initials: HR
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003673:Defective Viruses / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000254:growth & development*; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion
chemical_list: D012367:RNA, Viral