title: PI3-kinase regulates eosinophil and neutrophil degranulation in patients with allergic rhinitis and allergic asthma irrespective of allergen challenge model.
abstract: The PI3K pathway plays a major role in many vital cell processes. Our primary aim was to investigate signalling through PI3K for in vitro degranulation from allergen-primed eosinophils and neutrophils in allergic rhinitis and allergic asthma after seasonal and experimental allergen challenge. Nine patients with allergic rhinitis, eight with allergic asthma and four controls were studied during birch pollen season and after nasal and bronchial allergen challenge. Primed blood eosinophils and neutrophils were stimulated for in vitro degranulation with C3b-coated Sephadex particles, after prior incubation with Wortmannin, a PI3K inhibitor. The released amounts of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) were measured by radioimmunoassay. Wortmannin (10(-6) to 10(-9) M) inhibited ECP, EPO and MPO release in a dose-dependent manner in allergic rhinitis and allergic asthma in all three allergen challenge models. Inhibition of ECP release tended to be lower in the asthmatics in all allergen challenge models, statistically significant compared to the controls during season for 10(-8) M Wortmannin (p=0.01). A clear propensity towards less inhibition in the rhinitic patients was seen after nasal and bronchial challenge compared to seasonal exposure, significant for ECP (10(-8) M Wortmannin; p=0.034 and 0.002, respectively). Signalling through PI3K is clearly involved in ECP, EPO and MPO release in allergic rhinitis and allergic asthma irrespective of allergen challenge model. Allergic asthma demonstrated less inhibition of ECP release via PI3K during pollen season, indicating that other pathways play a greater role in eosinophil degranulation in allergic asthma than allergic rhinitis.
authors.affiliation: Department of Medical Sciences, Respiratory Medicine and Allergology, Uppsala University, SE-751 85, Uppsala, Sweden. mary.kampe@akademiska.se
authors.forename: Mary
authors.initials: M
authors.lastname: Kämpe
authors.forename: Maria
authors.initials: M
authors.lastname: Lampinen
authors.forename: Ingrid
authors.initials: I
authors.lastname: Stolt
authors.forename: Christer
authors.initials: C
authors.lastname: Janson
authors.forename: Gunnemar
authors.initials: G
authors.lastname: Stålenheim
authors.forename: Marie
authors.initials: M
authors.lastname: Carlson
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology; D000730:Androstadienes / Q000494:pharmacology; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D001985:Bronchial Provocation Tests; D015550:Cell Degranulation; D047150:Eosinophil Cationic Protein / Q000378:metabolism; D047209:Eosinophil Peroxidase / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009299:Nasal Provocation Tests; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000378:metabolism; D015398:Signal Transduction; D000077191:Wortmannin; D055815:Young Adult
chemical_list: D000485:Allergens; D000730:Androstadienes; D047150:Eosinophil Cationic Protein; D047209:Eosinophil Peroxidase; D009195:Peroxidase; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D000077191:Wortmannin