title: Postnatal elongation of eye size in DBA/2J mice compared with C57BL/6J mice: in vivo analysis with whole-eye OCT.
abstract: OBJECTIVE
  To characterize postnatal changes in eye size in glaucomatous DBA/2J (D2) mice and in nonglaucomatous C57BL/6J mice (B6) in vivo by means of whole-eye optical coherence tomography (OCT).
  
  
  METHODS
  D2 (n = 32) and B6 (n = 36) mice were tested between 2 and 20 months of age in eight age bins. A custom time-domain OCT system with a center wavelength of 825 nm and an axial scan length of 7.1 mm produced axial A-scan interferograms at a rate of 20 A-lines/s with a resolution of 8 μm. Axial length (AL), corneal thickness (CT), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), and retinal thickness (RT) were measured in the optical axis and adjusted with corresponding refractive indices. Corneal curvature (CC) and IOP were also measured.
  
  
  RESULTS
  AL increased (P < 0.001) more in the D2 (21%) than in the B6 (9%) mice. There was an interaction effect (two-way ANOVA, P < 0.001) between age and strain for AL, CT, ACD, and VCD. In the D2 mice, the lens became dislocated posteriorly. Multiple regression analysis in the D2 mice revealed an independent effect of age and IOP (P ≤ 0.01) on axial length. CC steepened in the older D2 mice, whereas it flattened in the B6 mice.
  
  
  CONCLUSIONS
  In D2 mice, postnatal elongation of AL is larger than that in B6 mice and is associated with a greater increase in ACD and IOP, which seems to be a causal factor. The ease of use, short acquisition time, and noninvasiveness of whole-eye OCT make it suitable for routine use in longitudinal studies of mouse models.
authors.affiliation: Bascom Palmer Eye Institute, University of Miami, Miller School of Medicine, Miami, Florida 33136, USA.
authors.forename: Tsung-Han
authors.initials: TH
authors.lastname: Chou
authors.forename: Omer P
authors.initials: OP
authors.lastname: Kocaoglu
authors.forename: David
authors.initials: D
authors.lastname: Borja
authors.forename: Marco
authors.initials: M
authors.lastname: Ruggeri
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Uhlhorn
authors.forename: Fabrice
authors.initials: F
authors.lastname: Manns
authors.forename: Vittorio
authors.initials: V
authors.lastname: Porciatti
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000867:Anterior Chamber / Q000473:pathology; D058452:Axial Length, Eye / Q000473:pathology*; D001699:Biometry; D001837:Body Weights and Measures; D003315:Cornea / Q000473:pathology; D005901:Glaucoma / Q000175:diagnosis*; D007429:Intraocular Pressure; D007908:Lens, Crystalline / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012160:Retina / Q000473:pathology; D041623:Tomography, Optical Coherence; D014065:Tonometry, Ocular; D014822:Vitreous Body / Q000473:pathology