title: Oxidative stress, DNA damage and osmolality in the Pacific white shrimp, Litopenaeus vannamei exposed to acute low temperature stress.
abstract: To evaluate the genotoxic, physiological and immunological effects of short-term acute low temperature stress on the Pacific white shrimp, Litopenaeus vannamei, we rapidly transferred shrimp from tanks at 23±2 °C to aquaria at the same temperature (controls) or 12±2 °C for 12 h. Changes in the shrimp hemocyte respiratory burst activity and DNA damage were examined during and after exposure to the temperature stress using flow cytometry and the comet assay, respectively. We also monitored changes in the total hemocyte count, malondialdehyde levels, total protein concentration and osmolality in shrimp plasma. The results show that hemocyte respiratory burst activity, malondialdehydes levels and hemocyte DNA damage in the plasma all increased significantly after exposure to 12±2 °C for 3 h. In contrast, total hemocyte count, total protein concentration and osmolality in the plasma decreased compared to the controls. We conclude that acute low temperature can induce oxidative stress, DNA damage, lipid peroxidation and changes in osmolality in L. vannamei.
authors.affiliation: Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, Guangzhou 510631, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qiu
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Wang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Liu
authors.forename: An-Li
authors.initials: AL
authors.lastname: Wang
mesh_terms: D000818:Animals; D019069:Cell Respiration; D003080:Cold Temperature; D020552:Comet Assay; D004249:DNA Damage; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000502:physiology*; D010140:Pacific Ocean; D033561:Penaeidae / Q000235:genetics / Q000502:physiology*; D011506:Proteins / Q000378:metabolism; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D011506:Proteins; D008315:Malondialdehyde