title: Ecotoxicoproteomics in gills of the sentinel fish species, Cottus gobio, exposed to perfluorooctane sulfonate (PFOS).
abstract: The environmental persistence, bioaccumulative tendency and potential toxicity of perfluorooctane sulfonate (PFOS) have generated great concern. This study aimed at evaluating the toxicity of short-term PFOS exposure in gills of the European bullhead Cottus gobio, a candidate sentinel species, by monitoring the response of some enzymes (citrate synthase CS, cytochrome c oxidase CCO, and lactate dehydrogenase LDH), and by undertaking a proteomic analysis using 2D-DIGE. First, a 96-h exposure to 1mg PFOS/L significantly altered the activity of mitochondrial CS and CCO. Second, 2D-DIGE gels were used to compare gills from the control fish group with tissues from fish exposed for 96h to either 0.1 or 1mg PFOS/L. From the 27 protein spots displaying significant changes in abundance following PFOS exposure, a total of 20 different proteins were identified using nano LC-MS/MS and the Peptide and Protein Prophet of Scaffold software. The differentially expressed proteins that were identified are involved in the general stress response, ubiquitin-proteasome system, energy metabolism, and actin cytoskeleton, which provide clues on the cellular pathways and components mainly affected by PFOS. Moreover, our results showed that most proteins were differentially expressed at the low but not at the high PFOS concentration. This work provides insights into the biochemical and molecular events in PFOS-induced toxicity in gill tissue, and suggests that further studies on the identified proteins could provide crucial information to better understand the mechanisms of PFOS toxicity in fish.
authors.affiliation: Unité de Recherche en Biologie des Organismes (URBO), The University of Namur (FUNDP), Rue de Bruxelles 61, B-5000 Namur, Belgium. jennifer.dorts@fundp.ac.be
authors.forename: Jennifer
authors.initials: J
authors.lastname: Dorts
authors.forename: Patrick
authors.initials: P
authors.lastname: Kestemont
authors.forename: Pierre-André
authors.initials: PA
authors.lastname: Marchand
authors.forename: Wendy
authors.initials: W
authors.lastname: D'Hollander
authors.forename: Marie-Laetitia
authors.initials: ML
authors.lastname: Thézenas
authors.forename: Martine
authors.initials: M
authors.lastname: Raes
authors.forename: Frédéric
authors.initials: F
authors.lastname: Silvestre
mesh_terms: D017738:Alkanesulfonic Acids / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D054750:Ecotoxicology; D004784:Environmental Monitoring / Q000379:methods; D029941:Fish Proteins / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D005466:Fluorocarbons / Q000378:metabolism / Q000633:toxicity*; D005880:Gills / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D017738:Alkanesulfonic Acids; D029941:Fish Proteins; D005466:Fluorocarbons; D020543:Proteome; D014874:Water Pollutants, Chemical; C076994:perfluorooctane sulfonic acid