title: [The expression of DNMT1 in laryngeal squamous cell carcinoma and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression of DNMT1 in laryngeal squamous cell carcinoma (LSCC) and its relationship with clinicopathological factors.
  
  
  METHODS
  Ninety-six cases of LSCC samples were detected by immunohistochemical staining to analyze the expression of DNMT1 protein, while 66 cases of them were detected by real-time PCR. And the relationship between the expression of DNMT1 mRNA and clinicopathologic factors was then analyzed.
  
  
  RESULTS
  The expression of DNMT1 mRNA in LSCC were up-regulated (P < 0.05). The positive rate of DNMT1 protein expression in LSCC and in peri-cancer tissue were 100% (96/96) and 36% (8/22) respectively, which showed a significant difference (P < 0.05). DNMT1 mRNA expression wasn't correlated with patients' age, gender, T stage and lympha node metastasis, but was associated with smoking habit (P < 0.05, respectively).
  
  
  CONCLUSIONS
  DNMT1 may initiate the oncogenesis of LSCC by increasing expression, and smoking habit may induce the expression of DNMT1 gene.
authors.affiliation: Department of Otolaryngology, Shengjing Hospital, China Medical University, Shenyang, 110004, China. wangjin068427@hotmail.com
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012907:Smoking
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human