title: Molecular cloning and characterization of a calreticulin cDNA from the pinewood nematode Bursaphelenchus xylophilus.
abstract: The cloning and characterization of a cDNA encoding a calreticulin from the pinewood nematode Bursaphelenchus xylophilus is described herein. The full-length cDNA (Bx-crt-1) contained a 1200 bp open reading frame that could be translated to a 399 amino acid polypeptide. The deduced protein contained highly conserved regions of a calreticulin gene and had 66.2-70.1% amino acid sequence identity to other calreticulin sequences from nematodes. RNAi, RT-PCR amplification, and southern blot suggest that Bx-crt-1 may be important for the development of B. xylophilus.
authors.affiliation: Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xundong
authors.initials: X
authors.lastname: Li
authors.forename: Kan
authors.initials: K
authors.lastname: Zhuo
authors.forename: Mei
authors.initials: M
authors.lastname: Luo
authors.forename: Longhua
authors.initials: L
authors.lastname: Sun
authors.forename: Jinling
authors.initials: J
authors.lastname: Liao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D037282:Calreticulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D028223:Pinus / Q000469:parasitology*; D010935:Plant Diseases / Q000469:parasitology*; D034622:RNA Interference; D018510:RNA, Helminth / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017187:Tylenchida / Q000737:chemistry / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D037282:Calreticulin; D017931:DNA Primers; D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth