title: Identification of cyclophilin A as a potential prognostic factor for clear-cell renal cell carcinoma by comparative proteomic analysis.
abstract: The high recurrence and improved survival rate of clear-cell renal cell carcinoma (ccRCC) demand for continuous effort to search for novel prognostic factors. Herein a comparative proteomics approach based on two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify differentially expressed proteins between ccRCC cell line RLC-310 and normal renal cell line HK-2. Of the 31 proteins identified, Cyclophilin A (CypA) was a newly identified differentially expressed protein in ccRCC cell line. The overexpression of CypA in ccRCC tissues was confirmed using RT-PCR and Western blot analyses. Further immunohistochemistry revealed that overexpression of CypA was associated with poor differentiation and decreased survival (p < 0.05, p < 0.0001). These data suggest that CypA may serve as a novel prognostic factor for ccRCC.
authors.affiliation: Medical Oncology Department; The First Affiliated Hospital, Xi'an Jiaotong University, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Yucong
authors.initials: Y
authors.lastname: Yang
authors.forename: Xu
authors.initials: X
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D021984:Cyclophilin A / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009363:Neoplasm Proteins; D021984:Cyclophilin A