title: [Dissociated mouse tooth germ epithelial cells retain the expression of tooth developmental genes during reaggregation process].
abstract: Generation of bio-engineered teeth by using stem cells will be a major approach for bioengineered implantation. Previous studies have demonstrated that dissociated tooth germ cells are capable of generating a tooth after reaggregation in vitro. However, the cellular and molecular mechanisms underlying this tooth regeneration are not clear. In this study, we dispersed E13.5 molar germ into single cells, immediately reaggregated them into cell pellet, then grafted the reaggregates under mouse kidney capsule for various times of culture. We investigated the morphogenesis and the expression of several developmental genes in dental epithelial cells in reaggregates of tooth germ cells. We found that dissociated tooth germ cells, after reaggregation, recapitulated normal tooth developmental process. In addition, dissociated dental epithelial cells retained the expression of Fgf8, Noggin, and Shh during reaggregation and tooth regeneration processes. Our results demonstrated that, despite of under dissociated status, dental epithelial cells maintained their odontogenic fate after re-aggregation with dental mesenchymal cells. These results provided important information for future in vitro generation of bio-engineered teeth from stem cells.
authors.affiliation: Key Laboratory of Developmental and Neuro Biology of Fujian Province, College of Life Sciences, Fujian Normal University, Fuzhou 350108, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Hu
authors.forename: Chensheng
authors.initials: C
authors.lastname: Lin
authors.forename: Bingmei
authors.initials: B
authors.lastname: Wang
authors.forename: Pingping
authors.initials: P
authors.lastname: Han
authors.forename: Yanding
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D008297:Male; D051379:Mice; D009805:Odontogenesis / Q000235:genetics*; D014083:Tooth Germ / Q000166:cytology / Q000502:physiology*