title: Low dose of gamma irradiation enhanced boronophenylalanine uptake in head and neck carcinoma cells for boron neutron capture therapy.
abstract: This study attempted to increase the boron uptake of human head and neck carcinoma SAS cells for BNCT by using a gamma dose of 0.1 Gy for combined treatment. Intracellular boron concentrations in 25 μgB/mL medium of BPA treated and BPA combined gamma-irradiation treated SAS cells were 73.8±1.73 and 95.15±1.36 ppm, respectively. After neutron irradiation, the G2/M-phase cell populations of untreated, BPA treated and BPA combined gamma-irradiation treated SAS cells were 19.31±1.71%, 52.47±2.25% and 59.19±2.63%, respectively. Experimental results indicate that the low dose gamma radiation with combination BPA treatment has the highest killing rate after neutron irradiation. Capable of significantly increasing the G2/M arrest after neutron irradiation, the combined treatment of a low dose of gamma irradiation with 25 μg B/mL medium of BPA also provided a higher killing effect for BNCT.
authors.affiliation: Institute of Nuclear Engineering and Science, National Tsing Hua University, Taiwan, Republic of China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Lin
authors.forename: S J
authors.initials: SJ
authors.lastname: Wang
authors.forename: H P
authors.initials: HP
authors.lastname: Chung
authors.forename: H M
authors.initials: HM
authors.lastname: Liu
authors.forename: F I
authors.initials: FI
authors.lastname: Chou
mesh_terms: D001896:Boron Compounds / Q000493:pharmacokinetics*; D016754:Boron Neutron Capture Therapy; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D002455:Cell Division; D004307:Dose-Response Relationship, Radiation; D016195:G2 Phase; D005720:Gamma Rays; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
chemical_list: D001896:Boron Compounds; D010649:Phenylalanine; C033685:4-boronophenylalanine