title: Topical gentamicin does not provide any additional anastomotic strength when combined with fibrin glue.
abstract: BACKGROUND
  We evaluated the effect of a combination of fibrin sealant and topical gentamicin on a colonic anastomosis in a rat model.
  
  
  METHODS
  Partial anastomosis in the transverse colon was performed in 70 male Sprague-Dawley rats aged 6 to 10 weeks using 5 interrupted sutures. The rats were divided into 4 groups (control, gentamicin, fibrin glue, and combination). On postoperative days 3 and 5, the rats in each group were killed, anastomotic bursting pressures scores and bowel loop adhesions were determined, and histologic examination was performed.
  
  
  RESULTS
  No significant difference was noted in the bursting pressures, adhesions, inflammatory infiltrates, fibroblasts, or neoangiogenesis between the fibrin-glue only and the combination groups for both the day 3 and day 5 subgroups.
  
  
  CONCLUSIONS
  The combination of topical gentamicin and fibrin glue had little effect because the combination did not provide additional anastomotic strength or decrease the number of adhesions when compared with fibrin glue alone.
authors.affiliation: Department of Surgery, Providence Hospital and Medical Centers, Southfield, MI 48075, USA.
authors.forename: Gokulakkrishna
authors.initials: G
authors.lastname: Subhas
authors.forename: Jasneet Singh
authors.initials: JS
authors.lastname: Bhullar
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cook
authors.forename: Asha
authors.initials: A
authors.lastname: Shah
authors.forename: Boris
authors.initials: B
authors.lastname: Silberberg
authors.forename: Lee
authors.initials: L
authors.lastname: Andrus
authors.forename: Melissa
authors.initials: M
authors.lastname: Decker
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Mittal
mesh_terms: D000287:Administration, Topical; D000714:Anastomosis, Surgical / Q000379:methods; D057868:Anastomotic Leak / Q000517:prevention & control*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003106:Colon / Q000601:surgery*; D004359:Drug Therapy, Combination; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology*; D006490:Hemostatics / Q000494:pharmacology; D008297:Male; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013536:Suture Techniques; D014014:Tissue Adhesives / Q000494:pharmacology*; D017211:Treatment Failure; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D015718:Fibrin Tissue Adhesive; D005839:Gentamicins; D006490:Hemostatics; D014014:Tissue Adhesives