title: Specific electromagnetic effects of microwave radiation on Escherichia coli.
abstract: The present study investigated the effects of microwave (MW) radiation applied under a sublethal temperature on Escherichia coli. The experiments were conducted at a frequency of 18 GHz and at a temperature below 40°C to avoid the thermal degradation of bacterial cells during exposure. The absorbed power was calculated to be 1,500 kW/m(3), and the electric field was determined to be 300 V/m. Both values were theoretically confirmed using CST Microwave Studio 3D Electromagnetic Simulation Software. As a negative control, E. coli cells were also thermally heated to temperatures up to 40°C using Peltier plate heating. Scanning electron microscopy (SEM) analysis performed immediately after MW exposure revealed that the E. coli cells exhibited a cell morphology significantly different from that of the negative controls. This MW effect, however, appeared to be temporary, as following a further 10-min elapsed period, the cell morphology appeared to revert to a state that was identical to that of the untreated controls. Confocal laser scanning microscopy (CLSM) revealed that fluorescein isothiocyanate (FITC)-conjugated dextran (150 kDa) was taken up by the MW-treated cells, suggesting that pores had formed within the cell membrane. Cell viability experiments revealed that the MW treatment was not bactericidal, since 88% of the cells were recovered after radiation. It is proposed that one of the effects of exposing E. coli cells to MW radiation under sublethal temperature conditions is that the cell surface undergoes a modification that is electrokinetic in nature, resulting in a reversible MW-induced poration of the cell membrane.
authors.affiliation: Faculty of Life and Social Sciences, Swinburne University, Melbourne, Victoria, Australia.
authors.forename: Yury
authors.initials: Y
authors.lastname: Shamis
authors.forename: Alex
authors.initials: A
authors.lastname: Taube
authors.forename: Natasa
authors.initials: N
authors.lastname: Mitik-Dineva
authors.forename: Rodney
authors.initials: R
authors.lastname: Croft
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
mesh_terms: D002462:Cell Membrane / Q000502:physiology / Q000528:radiation effects; D003911:Dextrans / Q000378:metabolism; D004926:Escherichia coli / Q000528:radiation effects* / Q000648:ultrastructure; D050296:Microbial Viability / Q000528:radiation effects; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D011827:Radiation; D013696:Temperature; D013997:Time Factors
chemical_list: D003911:Dextrans