title: Rapid acquisition of 1H and 19F NMR experiments for direct and competition ligand-based screening.
abstract: Direct and competition ligand-based NMR experiments are often used in the screening of chemical fragment libraries against a protein target due to the high relative sensitivity of NMR for protein-binding events. A plethora of NMR methods has been proposed for this purpose. Two of these techniques are the (19)F T(2) filter and the (1)H selective T(2) filter experiments. Modifications of the pulse sequences of these experiments have resulted in a ∼2-fold reduction in the experiment time thus allowing an increase in the screening throughput and making NMR an attractive technique for screening large compound collections.
authors.affiliation: Drug Discovery and Development, Italian Institute of Technology, Genova, Italy. claudio.dalvit@iit.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Dalvit
authors.forename: Alvar D
authors.initials: AD
authors.lastname: Gossert
authors.forename: Jérôme
authors.initials: J
authors.lastname: Coutant
authors.forename: Martial
authors.initials: M
authors.lastname: Piotto
mesh_terms: D001667:Binding, Competitive; D005461:Fluorine / Q000737:chemistry; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D012709:Serum Albumin / Q000737:chemistry; D012995:Solubility; D014364:Tryptophan / Q000737:chemistry
chemical_list: D008024:Ligands; D011522:Protons; D012709:Serum Albumin; D005461:Fluorine; D014364:Tryptophan