title: Expression of complement components, receptors and regulators by human dendritic cells.
abstract: Integration of innate and adaptive arms of the immune response at a cellular and molecular level appears to be fundamental to the development of powerful effector functions in host defence and aberrant immune responses. Here we provide evidence that the functions of human complement activation and antigen presentation converge on dendritic cells (DCs). We show that several subsets of human DCs [i.e., monocyte derived (CD1a(+)CD14(-)), dermal (CD1a(+)DC-SIGN(+)), Langerhans (CD1a(+)Langerin(+)), myeloid (CD1c(+)CD19(-)), plamacytoid (CD45RA(+)CD123(+))] express many of the components of the classical and alternative and terminal pathways of complement. Moreover human DCs have receptors known to detect the biologically active peptides C3a and C5a (C3aR, C5aR) and the covalently bound fragments C3b and metabolites iC3b and C3d which serve in immune adhesion (i.e., CR3, CR4, CRIg). We also show that the human DC surface is characterised by membrane bound regulators of complement activation, which are also known to participate in intracellular signalling (i.e., CD46, CD55, CD59). This work provides an extensive description of complement components relevant to the integrated actions of complement and DC, illuminated by animal studies. It acts as a resource that allows further understanding and exploitation of role of complement in human health and immune mediated diseases.
authors.affiliation: King's College London, MRC Centre for Transplantation, NIHR Comprehensive Biomedical Research Centre, Guy's Hospital, London, UK.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Henrieta
authors.initials: H
authors.lastname: Fazekasova
authors.forename: Naiyin
authors.initials: N
authors.lastname: Wang
authors.forename: Pervinder
authors.initials: P
authors.lastname: Sagoo
authors.forename: Qi
authors.initials: Q
authors.lastname: Peng
authors.forename: Wafa
authors.initials: W
authors.lastname: Khamri
authors.forename: Chantelle
authors.initials: C
authors.lastname: Gomes
authors.forename: Steven H
authors.initials: SH
authors.lastname: Sacks
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lombardi
authors.forename: Wuding
authors.initials: W
authors.lastname: Zhou
mesh_terms: D003165:Complement System Proteins / Q000235:genetics* / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology; D044967:Serum
chemical_list: D008070:Lipopolysaccharides; D011971:Receptors, Immunologic; D003165:Complement System Proteins