title: In vitro evidence for the protective role of Sida rhomboidea. Roxb extract against LDL oxidation and oxidized LDL-induced apoptosis in human monocyte-derived macrophages.
abstract: The present study was undertaken to evaluate protective role of S. rhomboidea. Roxb (SR) leaf extract against in vitro low-density lipoprotein (LDL) oxidation and oxidized LDL (Ox-LDL) induced macrophage apoptosis. Copper and cell-mediated LDL oxidation, Ox-LDL-induced peroxyl radical generation, mitochondrial activity, and apoptosis in human monocyte-derived macrophages (HMDMs) were assessed in presence of SR extract. Results clearly indicated that SR was capable of reducing LDL oxidation and formation of intermediary oxidation products. Also, SR successfully attenuated peroxyl radical formation, mitochondrial dysfunction, nuclear condensation, and apoptosis in Ox-LDL-exposed HMDMs. This scientific report is the first detailed investigation that establishes anti-atherosclerotic potential of SR extract.
authors.affiliation: Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, The M S University of Baroda, Vadodara, Gujarat, India.
authors.forename: Menaka C
authors.initials: MC
authors.lastname: Thounaojam
authors.forename: Ravirajsinh N
authors.initials: RN
authors.lastname: Jadeja
authors.forename: Ranjisinh V
authors.initials: RV
authors.lastname: Devkar
authors.forename: A V
authors.initials: AV
authors.lastname: Ramachandran
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors / Q000097:blood*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D019660:Malvaceae; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D008077:Lipoproteins, LDL; D010936:Plant Extracts; D020011:Protective Agents; C099252:oxidized low density lipoprotein