title: Human bone marrow adipocytes support dexamethasone-induced osteoclast differentiation and function through RANKL expression.
abstract: The TNF-family molecule, Receptor Activator of Nuclear factor κ B Ligand (RANKL) is known as a key regulator for bone remodeling, and is essential for the development and activation of osteoclasts. In this study, we examined the regulation of RANKL in primary human bone marrow adipocytes and the relationship between bone marrow adipocytes and bone metabolism. RANKL expression and the RANKL/osteoprotegerin (OPG) mRNA ratio in marrow adipocytes increased following dexamethasone treatment. In co-cultures of human osteoclast precursors and bone marrow adipocytes with dexamethasone, osteoclast precursors differentiated to TRAP-positive multinuclear cells. Moreover, the ability of bone resorption was confirmed in co-culture in flasks coated with calcium phosphate film. Osteoclast precursor differentiation and bone resorption were blocked by RANKL antibody pretreatment. TRAP-positive multinuclear cells did not form in coculture without cell-to-cell contact conditions. We conclude that primary human bone marrow adipocytes have the ability to promote osteoclast differentiation and activities, similar to osteoblasts and other RANKL-expressing cells.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Science, Nagasaki University, Japan.
authors.forename: Hisataka
authors.initials: H
authors.lastname: Goto
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukushima
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Hozumi
authors.forename: Hideo
authors.initials: H
authors.lastname: Baba
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shindo
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects*; D018920:Coculture Techniques; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D053245:RANK Ligand / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D003907:Dexamethasone; D016173:Macrophage Colony-Stimulating Factor