title: A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite.
abstract: In this paper, an amperometric biosensor of hydrogen peroxide (H(2)O(2)) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ*) and apparent heterogeneous electron transfer rate constant (k(s)) were calculated to be (1.60±0.17)×10(-10) mol cm(-2) and 48.51 s(-1), respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H(2)O(2). The biosensor of H(2)O(2) exhibited a linear response to H(2)O(2) in the range of 10-150 μM and a detection limit of 8.24 μM (S/N=3) was obtained. The apparent Michaelis-Menten constant (K(m)(app)) was 45.35 μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.
authors.affiliation: Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Xu
authors.forename: J X
authors.initials: JX
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Guo
authors.forename: W
authors.initials: W
authors.lastname: Zheng
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
authors.forename: B L
authors.initials: BL
authors.lastname: Wang
authors.forename: Y F
authors.initials: YF
authors.lastname: Zheng
authors.forename: Y B
authors.initials: YB
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Cheng
authors.forename: X
authors.initials: X
authors.lastname: Lou
authors.forename: B Z
authors.initials: BZ
authors.lastname: Jang
mesh_terms: D015374:Biosensing Techniques; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D055767:Immobilized Proteins / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D011060:Poloxalene / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D006454:Hemoglobins; D055767:Immobilized Proteins; C464484:pluronic block copolymer P123; D006108:Graphite; D011060:Poloxalene; D006861:Hydrogen Peroxide