title: Analogues of peptide SMAP-29 with comparable antimicrobial potency and reduced cytotoxicity.
abstract: SMAP-29 (sheep myeloid antimicrobial peptide-29) is a peptide with potent antibacterial properties. However, it is also highly cytotoxic both to human red blood cells (hRBCs) and human embryonic kidney (HEK) cells. In this study, some of the amino acids of SMAP-29 were changed in an attempt to reduce haemolytic activity whilst maintaining high antibacterial efficacy. These analogues, plus other analogues described in the literature with potent antimicrobial activity against Gram-positive bacteria coupled with no or low haemolytic activity, were evaluated for their cytotoxicity (hRBCs and HEK cells) as well as antimicrobial efficacy against two Gram-positive (Bacillus anthracis and Bacillus globigii) and two Gram-negative bacteria (Escherichia coli and Burkholderia thailandensis). The analogues previously described in the literature were found to have low antibacterial and haemolytic activity. Two of the designed analogues had comparable antibacterial efficacy with SMAP-29 against B. anthracis but reduced haemolytic activity and therefore had a therapeutic index that was enhanced 2.3-2.6-fold over that of SMAP-29.
authors.affiliation: DSTO Melbourne, 506 Lorimer Street, Fishermans Bend, VIC 3207, Australia. ray.dawson@dsto.defence.gov.au
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Dawson
authors.forename: Chun-Qiang
authors.initials: CQ
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D001407:Bacillus / Q000187:drug effects*; D001798:Blood Proteins / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D019117:Burkholderia / Q000187:drug effects*; D054804:Cathelicidins; D004847:Epithelial Cells / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology / Q000633:toxicity
chemical_list: D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D054804:Cathelicidins; D010455:Peptides; C403305:SMAP29 protein, Ovis aries