title: β-catenin in canine skin: immunohistochemical pattern of expression in normal skin and cutaneous epithelial tumours.
abstract: In normal adult skin, β-catenin is a structural component of the intercellular junction and the Wnt/β-catenin pathway plays a key role in the regulation of cutaneous homeostasis, particularly in the maintenance of hair follicle stem cells. No data are available on the expression pattern of β-catenin in normal canine skin and in canine cutaneous epidermal and follicular tumours. The present study used immunohistochemistry to determine β-catenin expression in four samples of normal canine skin and 62 cutaneous epithelial tumours (14 epidermal, 30 follicular and 18 glandular). β-catenin expression was localized to the nucleus of matrical and dermal papilla cells in anagen hair follicles and was also found in scattered cells of the outer root sheath, suggesting that these follicular epithelial cells may have a high proliferative potential. Nuclear labelling, considered a hallmark of activation of the Wnt/β-catenin signalling pathway, was observed in canine follicular tumours with matrical differentiation (100% of cases of trichoepithelioma and pilomatricoma), suggesting that a possible mutation of the canine CTNBB1 gene may underlie these tumours. In contrast, malignant tumours (squamous cell carcinoma, basal cell carcinoma, sebaceous and apocrine gland carcinoma and epithelioma) were characterized by reduction/loss of β-catenin membrane labelling compared with normal cutaneous epithelial cells and benign tumours, suggesting that reduction/loss of β-catenin expression is important in the acquisition of the malignant phenotype and may have a role in the infiltration and metastasis of these tumours.
authors.affiliation: Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. ldellasalda@unite.it
authors.forename: L
authors.initials: L
authors.lastname: Bongiovanni
authors.forename: D
authors.initials: D
authors.lastname: Malatesta
authors.forename: C
authors.initials: C
authors.lastname: Brachelente
authors.forename: S
authors.initials: S
authors.lastname: D'Egidio
authors.forename: L
authors.initials: L
authors.lastname: Della Salda
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D018294:Neoplasms, Adnexal and Skin Appendage / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000378:metabolism* / Q000473:pathology*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051176:beta Catenin