title: Identification of Helicobacter DNA in feline pancreas, liver, stomach, and duodenum: comparison between findings in fresh and formalin-fixed paraffin-embedded tissue samples.
abstract: The clinical significance of Helicobacter spp. in feline digestive organs needs to be evaluated and formalin-fixed and paraffin-embedded (FFPE) tissue samples provide an invaluable source for molecular studies. In this study, we performed a PCR assay to investigate the presence of Helicobacter DNA in digestive organs from seven cats and compared this occurrence in fresh and formalin-fixed and paraffin-embedded (FFPE) tissue samples from the same organs. The present study identified Helicobacter DNA in the pancreas, liver, stomach, and duodenum in fresh tissue samples but only in the stomach in FFPE samples. To our knowledge this is the first time that Helicobacter DNA have been identified in the feline pancreas. This study indicates that it is important to be aware of differences between results when analyzing FFPE samples compared to fresh tissue samples, especially regarding longer DNA fragments (>200 bp (base pairs)).
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Division of Bacteriology and Food Safety, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
authors.forename: Sofia
authors.initials: S
authors.lastname: Sjödin
authors.forename: Gunilla
authors.initials: G
authors.lastname: Trowald-Wigh
authors.forename: Mona
authors.initials: M
authors.lastname: Fredriksson
mesh_terms: D000818:Animals; D002415:Cats / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004386:Duodenum / Q000382:microbiology; D005557:Formaldehyde; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D008099:Liver / Q000382:microbiology*; D010179:Pancreas / Q000382:microbiology*; D016612:Paraffin Embedding / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013270:Stomach / Q000382:microbiology*; D016707:Tissue Fixation / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial; D005557:Formaldehyde