title: Moisture sorption characteristics of freeze-dried human platelets.
abstract: Freeze-drying is a promising method for a long-term storage of human platelets. The moisture sorption characteristics of freeze-dried human platelets (FDHPs) were studied in this paper. The moisture sorption isotherms of FDHPs and freeze-dried lyophilization buffer (FDLB) were measured at 4, 25, and 37 °C. The experimental data were fitted to Brunauer-Emmett-Teller (BET) and Guggenheim-Anderson-de Boer (GAB) equations. There were no significant statistical differences (P>0.05) between the sorption characteristics of FDHPs and FDLB at 4 and 25 °C, while FDHPs absorbed more water at 37 °C. The net isosteric heat of sorption was derived. The heat for FDHPs showed an abnormal negative value at low moisture contents when 25 and 37 °C data were used. Dynamic sorption experiments were carried out at 25 °C with environmental water activity controlled at 0.75, 0.85, and 0.90. The moisture diffusion coefficient was fitted to be 8.24×10(-12) m(2)/s when experimental data at initial time were used. These results would be helpful in choosing prehydration and storage condition for FDHPs.
authors.affiliation: Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China.
authors.forename: Meng-jie
authors.initials: MJ
authors.lastname: Xu
authors.forename: Guang-ming
authors.initials: GM
authors.lastname: Chen
authors.forename: Ju-li
authors.initials: JL
authors.lastname: Fan
authors.forename: Jin-hui
authors.initials: JH
authors.lastname: Liu
authors.forename: Xian-guo
authors.initials: XG
authors.lastname: Xu
authors.forename: Shao-zhi
authors.initials: SZ
authors.lastname: Zhang
mesh_terms: D000042:Absorption; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005612:Freeze Drying; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D015233:Models, Statistical; D015203:Reproducibility of Results; D013048:Specimen Handling; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water