title: Quantification of lurasidone, an atypical antipsychotic drug, in rat plasma with high-performance liquid chromatography with tandem mass spectrometry.
abstract: A liquid chromatography-tandem mass spectrometric (LC/MS/MS) method was developed for the determination of an atypical antipsychotic drug, lurasidone, in rat plasma. The method involves the addition of acetonitrile and ziprasidone (internal standard) solution to plasma samples, followed by centrifugation. An aliquot of the supernatant was diluted with water and directly injected into the LC/MS/MS system. The separations were performed on a column packed with octadecylsilica (5 μm, 2.0 × 50 mm) with 0.1% formic acid and 0.1% formic acid in acetonitrile as mobile phase and the detection was performed using tandem mass spectrometry by multiple-reaction monitoring via an electrospray ionization source. The standard curve was linear (r = 0.9982) over the concentration range 0.002-1 μg/mL. The intra- and inter-assay precisions were 1.7 and 8.6%, respectively. The accuracy range was from 90.3 to 101.8%. The lower limit of quantification was 2.0 ng/mL using 50 μL of rat plasma sample. The developed analytical method was successfully applied to the pharmacokinetic study of lurasidone in rats.
authors.affiliation: Life Science R&D Park, SK Holdings Co. Ltd, Daejeon, 305-712, Korea. kootae@sk.com
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Koo
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jongjoo
authors.initials: J
authors.lastname: Lee
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Ha
authors.forename: Myoungki
authors.initials: M
authors.lastname: Baek
authors.forename: Hongsik
authors.initials: H
authors.lastname: Moon
mesh_terms: D000097:Acetonitriles / Q000097:blood; D000818:Animals; D014150:Antipsychotic Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054833:Isoindoles / Q000097:blood* / Q000493:pharmacokinetics; D016014:Linear Models; D000069056:Lurasidone Hydrochloride; D008297:Male; D010879:Piperazines / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013844:Thiazoles / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000097:Acetonitriles; D014150:Antipsychotic Agents; D054833:Isoindoles; D010879:Piperazines; D013844:Thiazoles; C092292:ziprasidone; D000069056:Lurasidone Hydrochloride; C032159:acetonitrile