title: In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly-L-lysine-treated titanium-6-aluminium-4-vanadium.
abstract: Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V.
authors.affiliation: Interdepartmental Center of Tissue Engineering, University of Pavia, Italy. dgalli05@yahoo.it
authors.forename: Daniela
authors.initials: D
authors.lastname: Galli
authors.forename: Laura
authors.initials: L
authors.lastname: Benedetti
authors.forename: Matilde
authors.initials: M
authors.lastname: Bongio
authors.forename: Valentina
authors.initials: V
authors.lastname: Maliardi
authors.forename: Giulia
authors.initials: G
authors.lastname: Silvani
authors.forename: Gabriele
authors.initials: G
authors.lastname: Ceccarelli
authors.forename: Flavio
authors.initials: F
authors.lastname: Ronzoni
authors.forename: Silvio
authors.initials: S
authors.lastname: Conte
authors.forename: Francesco
authors.initials: F
authors.lastname: Benazzo
authors.forename: Antonio
authors.initials: A
authors.lastname: Graziano
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Papaccio
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.forename: Maria Gabriella Cusella
authors.initials: MG
authors.lastname: De Angelis
mesh_terms: D000497:Alloys; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D002454:Cell Differentiation; D017931:DNA Primers / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D066298:In Vitro Techniques; D019012:Integrin beta1 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D010766:Phosphorylation; D011107:Polylysine / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D017931:DNA Primers; D019012:Integrin beta1; C031462:titanium alloy (TiAl6V4); D011107:Polylysine; D014025:Titanium; D051416:Focal Adhesion Protein-Tyrosine Kinases