title: Bioconjugation of L-3,4-dihydroxyphenylalanine containing protein with a polysaccharide.
abstract: We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation of 3,4-dihydroxy-l-phenylalanine can alter the properties of protein to conjugate into the polymers. The homogeneously modified protein will yield quinone residues that are covalently conjugated to nucleophilic groups of the amino polysaccharide. This novel approach holds great promise for widespread use to prepare protein conjugates and synthetic biology applications.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, South Korea.
authors.forename: Niraikulam
authors.initials: N
authors.lastname: Ayyadurai
authors.forename: Nadarajan Saravanan
authors.initials: NS
authors.lastname: Prabhu
authors.forename: Kanagavel
authors.initials: K
authors.lastname: Deepankumar
authors.forename: Yoon Jung
authors.initials: YJ
authors.lastname: Jang
authors.forename: Nataraj
authors.initials: N
authors.lastname: Chitrapriya
authors.forename: Eunjung
authors.initials: E
authors.lastname: Song
authors.forename: Nahum
authors.initials: N
authors.lastname: Lee
authors.forename: Seog K
authors.initials: SK
authors.lastname: Kim
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.forename: Nagasundarapandian
authors.initials: N
authors.lastname: Soundrarajan
authors.forename: Sungu
authors.initials: S
authors.lastname: Lee
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
authors.forename: Nediljko
authors.initials: N
authors.lastname: Budisa
authors.forename: Hyungdon
authors.initials: H
authors.lastname: Yun
mesh_terms: D007980:Levodopa / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D011506:Proteins; D007980:Levodopa