title: Inhibitory effect of hydro-methanolic extract of seed of Holarrhena antidysenterica on alpha-glucosidase activity and postprandial blood glucose level in normoglycemic rat.
abstract: BACKGROUND
  The present experiment was conducted to search out the effect of hydro-methanolic extract of seed of Holarrhena antidysenterica on intestinal α-glucosidase activity in dose dependent manner and on the management of postprandial hyperglycemia in starch loaded rats.
  
  
  METHODS
  Activity of intestinal α-glucosidase was measured by in vitro method. Fasting blood glucose level was determined by single touch glucometer. Total phenol and flavonoids of seed extract of Holarrhena antidysenterica were estimated using gallic acid and quercetin standard curves, respectively.
  
  
  RESULTS
  The degree of elevation in blood glucose level after starch administration was significantly (p<0.05) less by the extract in respect to the control. The said extract also inhibited α-glucosidase activity having an IC(50) of 0.52mg/ml. Phytochemical study revealed that the extract is rich in phenolic compounds (60.23mg of gallic acid equivalent/g of extract) and flavonoids (360.23mg of quercetin equivalent/g of the extract).
  
  
  CONCLUSIONS
  The extract exerts its antihyperglycemic effect by retarding the carbohydrate absorption from intestine through the inhibition in α-glucosidase activity and therefore resists postprandial hyperglycemia.
authors.affiliation: Bio-Medical Laboratory Science and Management (U.G.C. Innovative Funded Department), Vidyasagar University, Midnapore 721 102, West Bengal, India.
authors.forename: Kazi Monjur
authors.initials: KM
authors.lastname: Ali
authors.forename: Kausik
authors.initials: K
authors.lastname: Chatterjee
authors.forename: Debasis
authors.initials: D
authors.lastname: De
authors.forename: Kishalay
authors.initials: K
authors.lastname: Jana
authors.forename: Tushar Kanti
authors.initials: TK
authors.lastname: Bera
authors.forename: Debidas
authors.initials: D
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D035462:Holarrhena / Q000737:chemistry*; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D019518:Postprandial Period; D051381:Rats; D012016:Reference Values; D012639:Seeds; D013213:Starch / Q000008:administration & dosage / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D059808:Polyphenols; D013213:Starch; D000520:alpha-Glucosidases