title: Comparative effects of conjugated linoleic acid (CLA) and linoleic acid (LA) on the oxidoreduction status in THP-1 macrophages.
abstract: The aim of this study was to investigate the effect of conjugated linoleic acids (CLAs) on macrophage reactive oxygen species synthesis and the activity and expression of antioxidant enzymes, catalase (Cat), glutathione peroxidase (GPx), and superoxide dismutase (SOD). The macrophages were obtained from the THP-1 monocytic cell line. Cells were incubated with the addition of cis-9,trans-11 CLA or trans-10,cis-12 CLA or linoleic acid. Reactive oxygen species (ROS) formation was estimated by flow cytometry. Enzymes activity was measured spectrophotometrically. The antioxidant enzyme mRNA expression was estimated by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Statistical analysis was based on nonparametric statistical tests [Friedman analysis of variation (ANOVA) and Wilcoxon signed-rank test]. cis-9,trans-11 CLA significantly increased the activity of Cat, while trans-10,cis-12 CLA notably influenced GPx activity. Both isomers significantly decreased mRNA expression for Cat. Only trans-10,cis-12 significantly influenced mRNA for SOD-2 expression. The CLAs activate processes of the ROS formation in macrophages. Adverse metabolic effects of each isomer action were observed.
authors.affiliation: Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland. rcmarta@wp.pl
authors.forename: Marta
authors.initials: M
authors.lastname: Rybicka
authors.forename: Ewa
authors.initials: E
authors.lastname: Stachowska
authors.forename: Izabela
authors.initials: I
authors.lastname: Gutowska
authors.forename: Miłosz
authors.initials: M
authors.lastname: Parczewski
authors.forename: Magdalena
authors.initials: M
authors.lastname: Baśkiewicz
authors.forename: Bogusław
authors.initials: B
authors.lastname: Machaliński
authors.forename: Anna
authors.initials: A
authors.lastname: Boroń-Kaczmarska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
mesh_terms: D000704:Analysis of Variance; D001483:Base Sequence; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D017931:DNA Primers; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D019787:Linoleic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase