title: Uncovering the vasorelaxant effect induced by Vale do São Francisco red wine: a role for nitric oxide.
abstract: The aim of this study was to investigate the mechanisms underlying the vasorelaxant effect induced by the polyphenolic compounds found in red wine from Vale do São Francisco. In phenylephrine (10 μM) precontracted mesenteric artery rings, the red wine caused a concentration-dependent relaxation (maximum response to phenylephrine 10 μM = 87.5% ± 6.5%, n = 10). After endothelium removal, the vasorelaxant effect elicited by red wine was attenuated (28.4% ± 4.9%, n = 10). In addition, the vasorelaxant effect induced by red wine in rings pretreated with 100 μM of N(w)-nitro-l-arginine methyl ester and 10 μM of 1H-[1,2,4] oxadiazolo-[4,3-a]-quinoxalin-1-one was attenuated (23.4% ± 5.1%, n = 7 and 11.8% ± 2.7%, n = 6, respectively). Pretreatment with atropine did not affect the vasorelaxant effect induced by red wine (81% ± 3.9%, n = 6). Furthermore, in rabbit aortic endothelial cell line, red wine 100 and 300 μg/mL caused concentration-dependent increases in nitric oxide levels (58 ± 1; 82 ± 7.9; Δ% of fluorescence, n = 5, respectively). In conclusion, we suggest that the alcohol free-lyophilized red wine induces an endothelium-dependent vasorelaxant effect due, at least in part, to a secondary increase in the concentration of nitric oxide and that this effect might be associated with phenolic compounds found in the red wine.
authors.affiliation: Laboratory of Pharmaceutical Technology, Federal University of Parana, Curitiba, Brazil.
authors.forename: Melissa N
authors.initials: MN
authors.lastname: Luciano
authors.forename: Thaís P
authors.initials: TP
authors.lastname: Ribeiro
authors.forename: Maria S
authors.initials: MS
authors.lastname: França-Silva
authors.forename: Roberto J B
authors.initials: RJ
authors.lastname: do Nascimento
authors.forename: Eduardo
authors.initials: E
authors.lastname: de Jesus Oliveira
authors.forename: Karime C
authors.initials: KC
authors.lastname: França
authors.forename: Alessandra A
authors.initials: AA
authors.lastname: Antunes
authors.forename: Lia S
authors.initials: LS
authors.lastname: Nakao
authors.forename: Carlos A M
authors.initials: CA
authors.lastname: Aita
authors.forename: Valdir A
authors.initials: VA
authors.lastname: Braga
authors.forename: Isac A
authors.initials: IA
authors.lastname: de Medeiros
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism; D001938:Brazil; D002460:Cell Line; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005612:Freeze Drying; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D008297:Male; D017538:Mesenteric Artery, Superior / Q000187:drug effects*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D059808:Polyphenols; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014665:Vasodilator Agents / Q000494:pharmacology*; D014920:Wine / Q000032:analysis*
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D014665:Vasodilator Agents; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D006162:Guanylate Cyclase