title: Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri.
abstract: We studied the effect of triterpenoid saponins on the development of free-living stages of Heligmosomoides bakeri, a parasitic nematode of the mouse intestine. We evaluated the effectiveness of oleane-type glucuronides (GlcUAOA) isolated from Calendula officinalis and Beta vulgaris. The rhodamine 123 retention assay was used to detect dysfunctions of the major membrane transporter for xenobiotics, P-glycoprotein (Pgp). Both C. officinals and B. vulgaris GlcUAOA affect the development of the free living stages and function of Pgp in H. bakeri. The GlcUAOA inhibits egg hatching and moulting of larvae and also changes their morphology. These saponin fractions reversed the toxic effect of thiabendazole on the nematode; the function of Pgp was also inhibited.
authors.affiliation: Institute of Zoology, University of Warsaw, Warsaw, Poland. mdolig@biol.uw.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Doligalska
authors.forename: K
authors.initials: K
authors.lastname: Jóźwicka
authors.forename: M
authors.initials: M
authors.lastname: Kiersnowska
authors.forename: A
authors.initials: A
authors.lastname: Mroczek
authors.forename: C
authors.initials: C
authors.lastname: Pączkowski
authors.forename: W
authors.initials: W
authors.lastname: Janiszowska
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D027461:Beta vulgaris / Q000737:chemistry; D020838:Calendula / Q000737:chemistry; D020719:Glucuronides / Q000737:chemistry / Q000494:pharmacology*; D006369:Heligmosomatoidea / Q000187:drug effects* / Q000502:physiology; D007814:Larva / Q000187:drug effects; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D020719:Glucuronides; D012503:Saponins; D014315:Triterpenes; D014700:Verapamil