title: Applications and recent developments of multi-analyte simultaneous analysis by enzyme-linked immunosorbent assays.
abstract: The recent development and application of multi-analyte simultaneous analysis by enzyme-linked immunosorbent assays (ELISAs) of many kinds of organic compounds are reviewed herein. Multi-analyte ELISAs based on generic antibodies directly, or on specific antibodies by multi-antibody co-coating or multi-tracer co-marking are described in detail. Special attention was placed on the design and synthesis of a generic hapten, the manner of linking the hapten to a carrier protein, type and character of the antibody and format of the ELISAs. It is hoped that this comprehensive review can be considered as a useful reference for further investigation of multi-analyte simultaneous analysis by ELISAs.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, PR China. shwzhhy@163.com
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Guozhen
authors.initials: G
authors.lastname: Fang
mesh_terms: D000906:Antibodies / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000032:analysis*
chemical_list: D000906:Antibodies; D006241:Haptens