title: Efficient, non-toxic hybrid PPV-PAMAM dendrimer as a gene carrier for neuronal cells.
abstract: A novel hybrid dendrimer (TRANSGEDEN) that combines a conjugated rigid polyphenylenevinylene (PPV) core with flexible polyamidoamine (PAMAM) branches at the surface was synthesized and characterized. The potential of this material as a nonviral gene delivery system was also examined, and it was observed that dendriplexes formed by TRANSGEDEN and small interfering ribonucleic acids (siRNAs) can be incorporated into >90% of neuronal cells without any toxicity up to a dendrimer concentration of 3 μM. TRANSGEDEN was used to deliver a specific siRNA to rat cerebellar granular neurons (CGNs) to knock down the cofilin-1 protein. Cofilin-1 removal partially protects CGNs from N-methyl D-aspartate (NMDA)-mediated neuronal death.
authors.affiliation: Facultad de Química, Universidad de Castilla-La Mancha, Avda Camilo José Cela 10, 13071-Ciudad Real, Spain.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Rodrigo
authors.forename: Iván
authors.initials: I
authors.lastname: Rivilla
authors.forename: Francisco C
authors.initials: FC
authors.lastname: Pérez-Martínez
authors.forename: Silvia
authors.initials: S
authors.lastname: Monteagudo
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ocaña
authors.forename: Javier
authors.initials: J
authors.lastname: Guerra
authors.forename: Joaquín C
authors.initials: JC
authors.lastname: García-Martínez
authors.forename: Sonia
authors.initials: S
authors.lastname: Merino
authors.forename: Prado
authors.initials: P
authors.lastname: Sánchez-Verdú
authors.forename: Valentín
authors.initials: V
authors.lastname: Ceña
authors.forename: Julián
authors.initials: J
authors.lastname: Rodríguez-López
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D050091:Dendrimers / Q000737:chemistry*; D005822:Genetic Vectors; D009682:Magnetic Resonance Spectroscopy; D009474:Neurons / Q000378:metabolism*; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D034741:RNA, Small Interfering