title: Transplantation of a mammary stromal cell line into a mammary fat pad: development of the site-specific in vivo analysis system for mammary stromal cells.
abstract: The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Nakatani
authors.forename: Naohito
authors.initials: N
authors.lastname: Aoki
authors.forename: Daita
authors.initials: D
authors.lastname: Nadano
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D001391:Azo Compounds / Q000032:analysis; D002329:Carmine / Q000032:analysis; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D004848:Epithelium / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D005697:Galactosides / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007150:Immunohistochemistry; D007211:Indoles / Q000378:metabolism; D007633:Keratins / Q000032:analysis; D008321:Mammary Glands, Animal / Q000166:cytology / Q000254:growth & development / Q000637:transplantation*; D051379:Mice; D009940:Organoids / Q000166:cytology / Q000637:transplantation*; D019899:Paracrine Communication; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D014170:Transformation, Genetic; D014185:Transplantation, Isogeneic
chemical_list: D001391:Azo Compounds; D005697:Galactosides; D007211:Indoles; D007633:Keratins; D002329:Carmine; C011049:oil red O; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside