title: A bioinformatic assay for pluripotency in human cells.
abstract: Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.
authors.affiliation: Zentrum für Integrative Psychiatrie, Kiel, Germany. fj.mueller@zip-kiel.de
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Müller
authors.forename: Bernhard M
authors.initials: BM
authors.lastname: Schuldt
authors.forename: Roy
authors.initials: R
authors.lastname: Williams
authors.forename: Dylan
authors.initials: D
authors.lastname: Mason
authors.forename: Gulsah
authors.initials: G
authors.lastname: Altun
authors.forename: Eirini P
authors.initials: EP
authors.lastname: Papapetrou
authors.forename: Sandra
authors.initials: S
authors.lastname: Danner
authors.forename: Johanna E
authors.initials: JE
authors.lastname: Goldmann
authors.forename: Arne
authors.initials: A
authors.lastname: Herbst
authors.forename: Nils O
authors.initials: NO
authors.lastname: Schmidt
authors.forename: Josef B
authors.initials: JB
authors.lastname: Aldenhoff
authors.forename: Louise C
authors.initials: LC
authors.lastname: Laurent
authors.forename: Jeanne F
authors.initials: JF
authors.lastname: Loring
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008957:Models, Genetic; D009474:Neurons; D020411:Oligonucleotide Array Sequence Analysis; D039904:Pluripotent Stem Cells / Q000502:physiology*; D012984:Software