title: Role of cholesterol 24S-hydroxylase gene polymorphism (rs754203) in primary open angle glaucoma.
abstract: OBJECTIVE
  The enzyme cholesterol 24S-hydroxylase (Cyp46A1) is responsible for the conversion of cholesterol to its more polar metabolite 24S-hydroxycholesterol, thereby enabling the intracerebral elimination of cholesterol. An intronic single nucleotide polymorphism in the gene CYP46A1 (IVS2 -150 T>C; rs754203) has recently been associated with primary open angle glaucoma (POAG). This association, however, lacks confirmation in other studies. The purpose of the present study was to investigate a hypothesized association between rs754203 and the presence of POAG in a Central European population of Caucasian descent.
  
  
  METHODS
  The present institutional study comprised a total of 581 unrelated subjects: 330 patients with POAG, and 251 control subjects. Main outcome measures are genotype distributions and allelic frequencies determined by polymerase chain reaction.
  
  
  RESULTS
  No significant differences in either genotype distribution or allelic frequencies were found between patients with POAG and control subjects (p>0.05). The presence of the rs754203 T-allele was associated with a nonsignificant odds ratio of 0.81 (95% CI: 0.63-1.04; p=0.11) for POAG.
  
  
  CONCLUSIONS
  Our data suggest that the rs754203 polymorphism itself is unlikely a genetic risk factor for POAG in Caucasian individuals.
authors.affiliation: Department of Ophthalmology, Medical University of Graz, Graz, Austria. g.mossboeck@medunigraz.at
authors.forename: Georg
authors.initials: G
authors.lastname: Mossböck
authors.forename: Martin
authors.initials: M
authors.lastname: Weger
authors.forename: Christoph
authors.initials: C
authors.lastname: Faschinger
authors.forename: Otto
authors.initials: O
authors.lastname: Schmut
authors.forename: Wilfried
authors.initials: W
authors.lastname: Renner
authors.forename: Andreas
authors.initials: A
authors.lastname: Wedrich
authors.forename: Christina
authors.initials: C
authors.lastname: Zimmermann
authors.forename: Yosuf
authors.initials: Y
authors.lastname: El-Shabrawi
mesh_terms: D000368:Aged; D000072556:Cholesterol 24-Hydroxylase; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005902:Glaucoma, Open-Angle / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*
chemical_list: D013250:Steroid Hydroxylases; D000072556:Cholesterol 24-Hydroxylase