title: Improvement of the production of a red pigment in Penicillium sp. HSD07B synthesized during co-culture with Candida tropicalis.
abstract: Co-culture of Penicillium sp. HSD07B and Candida tropicalis resulted in the production of a red pigment consisting of six components as determined by TLC and HPLC. The pigment showed no acute toxicity in mice and was mot mutagenic in the Ames test. The pigment was stable between pH 2 and 10 and temperatures of 10-100°C and exhibited good photo-stability and resistance to oxidization by hydrogen peroxide and reduction by Na(2)SO(3). Glucose and ratio of C. tropicalis to strain HSD07B (w/w) in the inoculum were the important factors influencing production of the pigment. Under optimized conditions, a pigment yield of 2.75 and 7.7 g/l was obtained in a shake-flask and a 15 l bioreactor, respectively. Thus, co-culture of strain HSD07B and C. tropicalis is a promising way to produce a red pigment potentially useful for coloring applications.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China. whl@htu.cn
authors.forename: Wang
authors.initials: W
authors.lastname: Hailei
authors.forename: Ren
authors.initials: R
authors.lastname: Zhifang
authors.forename: Li
authors.initials: L
authors.lastname: Ping
authors.forename: Gu
authors.initials: G
authors.lastname: Yanchang
authors.forename: Liu
authors.initials: L
authors.lastname: Guosheng
authors.forename: Yao
authors.initials: Y
authors.lastname: Jianming
mesh_terms: D000818:Animals; D041022:Candida tropicalis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D018920:Coculture Techniques; D051379:Mice; D010407:Penicillium / Q000378:metabolism*; D010860:Pigments, Biological / Q000096:biosynthesis*
chemical_list: D010860:Pigments, Biological