title: Characterization of three cell lines derived from fine needle biopsy of choroidal melanoma with metastatic outcome.
abstract: OBJECTIVE
  To report three low-passage cell lines from primary choroidal melanoma with metastatic outcome, which were stable for cytogenetic patterns and expression profiles of the primary melanoma.
  
  
  METHODS
  In patients with choroidal melanoma, transscleral fine needle aspiration biopsy (FNAB) was performed immediately before plaque placement for (125)iodine brachytherapy or immediately after enucleation. Cells were examined for cytopathology, evaluated by fluorescence in-situ hybridization (FISH) for the centromere of chromosome 3, analyzed by 250K whole genome Mapping Array and U133 plus 2.0 Expression Array, and placed in cell culture. At passage 3, the cell lines were analyzed by Mapping Array and Expression Array.
  
  
  RESULTS
  Three cell lines were propagated from primary choroidal melanomas in three patients who subsequently developed metastasis. Two cell lines were stable for the entire chromosomal aberration pattern of the respective primary tumor. In the third, necrotic material from the biopsy prevented further analysis, yet resulted in a stable cell line. Each cell line had chromosome 3 loss, 6q loss, 8p loss, multiple 8q gain, and 16q loss. Additionally, two cell lines had chromosome 6p gain. Two cell lines had RNA expression profiles similar to the respective primary tumors; the third cell line had a similar RNA expression profile relative to the other two cell lines.
  
  
  CONCLUSIONS
  FNAB of primary choroidal melanomas resulted in highly characterized, low-passage cell lines, which were stable for the cytogenetic patterns and expression profiles found in the primary tumor. These cell lines represent novel tools for the study of metastatic choroidal melanoma biology.
authors.affiliation: Department of Ophthalmology, University of California, Los Angeles, The Jules Stein Eye Institute, Los Angeles, CA, USA.
authors.forename: Barry L
authors.initials: BL
authors.lastname: Burgess
authors.forename: Nagesh P
authors.initials: NP
authors.lastname: Rao
authors.forename: Ascia
authors.initials: A
authors.lastname: Eskin
authors.forename: Stanley F
authors.initials: SF
authors.lastname: Nelson
authors.forename: Tara A
authors.initials: TA
authors.lastname: McCannel
mesh_terms: D001483:Base Sequence; D044963:Biopsy, Fine-Needle; D045744:Cell Line, Tumor / Q000473:pathology*; D002829:Choroid / Q000378:metabolism / Q000473:pathology*; D002830:Choroid Neoplasms / Q000235:genetics / Q000473:pathology*; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D015536:Down-Regulation / Q000235:genetics; D044385:GTP-Binding Protein alpha Subunits / Q000235:genetics; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D018628:Gene Dosage / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics / Q000473:pathology*; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009006:Monosomy / Q000235:genetics; D009362:Neoplasm Metastasis; D015854:Up-Regulation / Q000235:genetics
chemical_list: D003062:Codon; C554061:GNAQ protein, human; D044385:GTP-Binding Protein alpha Subunits; D043802:GTP-Binding Protein alpha Subunits, Gq-G11