title: Identification and determination of flavonoids in astragali radix by high performance liquid chromatography coupled with DAD and ESI-MS detection.
abstract: A method for the analysis of flavonoids in Astragali Radix by high-performance liquid chromatography (HPLC) combined with photodiode-array detection (DAD) and an electrospray ionization (ESI)--mass spectrometry (MS) was developed. After the samples were extracted with ethanol, the optimum separation conditions for these analytes were achieved using a gradient elution system and a 2.0 x 150 mm Shimadzu VP-ODS column. Eight flavonoids were identified to exist in Astragali Radix based on their characteristic UV data and mass spectra. The concentrations of three major components in this herb--ononin, calycosin and formononetin--were determined by LC/ESI-MS in positive selective ion monitoring (SIM) mode. The calibration curves were linear in the range of 0.9~180.0 μg·mL⁻¹ for ononin, 1.8~360.0 μg·mL⁻¹ for calycosin and 1.4~280 μg·mL⁻¹ for formononetin, respectively. The limits of quantification (LOQ) and detection (LOD) were 0.9 μg· mL⁻¹ and 0.2 μg mL⁻¹ for ononin, 1.8 μg mL⁻¹ and 0.5 μg·mL-1 for calycosin, 1.4 μg mL⁻¹ and 0.5 μg·mL⁻¹ for formononetin, respectively. The standard recoveries were between 95.4~104.7%. The developed method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in various resources of Astragali Radix.
authors.affiliation: Department of Chemistry and Material Engineering, Quzhou College, Quzhou 324000, China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Lan-Fang
authors.initials: LF
authors.lastname: Huang
authors.forename: Yun-Biao
authors.initials: YB
authors.lastname: He
authors.forename: Xian-Zhen
authors.initials: XZ
authors.lastname: Xie
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D005419:Flavonoids; D010936:Plant Extracts