title: Development of a fibrinogen-specific sandwich enzyme-linked immunosorbent assay microarray assay for distinguishing between blood plasma and serum samples.
abstract: We have developed a fibrinogen-specific sandwich enzyme-linked immunosorbent assay (ELISA) microarray assay for use in qualitatively distinguishing between blood plasma and serum samples. Three capture antibodies (49D2, HPA001900, and F8512) were evaluated in conjunction with 1D6 as the detection antibody. The data show that 49D2 and (to a lesser extent) F8512 successfully identify previously unknown plasma and serum samples based on approximately a 28-fold difference in signal intensity between the sample types. This assay has utility in rapidly identifying previously archived clinical samples with incomplete annotation in a high-throughput manner prior to proteomic analyses.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Gonzalez
authors.forename: Qibin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Richard C
authors.initials: RC
authors.lastname: Zangar
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D005340:Fibrinogen / Q000032:analysis* / Q000276:immunology; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D040081:Protein Array Analysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000191:economics / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D005340:Fibrinogen