title: Characterization of bionanocomposite scaffolds comprised of amine-functionalized gold nanoparticles and silicon carbide nanowires crosslinked to an acellular porcine tendon.
abstract: As one of the most common proteins found in the human body, collagen is regarded as biocompatible and has many properties making it ideal for soft-tissue repair applications. However, collagen matrices fabricated from purified forms of collagen are notoriously weak and easily degraded by the body. The extracellular matrix of many tissues including human dermis, porcine dermis, and porcine small intestine submucosa are often utilized instead, and several of these scaffolds are crosslinked. Crosslinking has been shown to improve the mechanical properties of collagenous tissues and increase their resistance to degradation. In this study we investigated two novel "bionanocomposite" materials in which either gold nanoparticles or silicon carbide nanowires were crosslinked to a porcine tendon. Scanning electron micrographs confirmed that the nanomaterials were successfully crosslinked to the tissues. A collagenase assay, tensile testing, flow cytometry, and bioreactor studies were also performed to further characterize the properties of these novel materials. The results of these studies indicated that crosslinking porcine diaphragm tissues with nanomaterials resulted in scaffolds with improved resistance to enzymatic degradation and appropriate biocompatibility characteristics, thus warranting further study of these materials for soft tissue repair and tissue engineering applications.
authors.affiliation: Department of Surgery, Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: Corey R
authors.initials: CR
authors.lastname: Deeken
authors.forename: Derek B
authors.initials: DB
authors.lastname: Fox
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Bachman
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Ramshaw
authors.forename: Sheila A
authors.initials: SA
authors.lastname: Grant
mesh_terms: D000588:Amines / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D019149:Bioreactors; D017554:Carbon Compounds, Inorganic / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry*; D013552:Swine; D013710:Tendons / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000588:Amines; D001672:Biocompatible Materials; D017554:Carbon Compounds, Inorganic; D003432:Cross-Linking Reagents; D017655:Silicon Compounds; D006046:Gold; C022088:silicon carbide