title: Determination of triamcinolone in human plasma by a sensitive HPLC-ESI-MS/MS method: application for a pharmacokinetic study using nasal spray formulation.
abstract: A liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantitation of triamcinolone in human plasma after nasal spray application was developed and validated. Betamethasone was used as internal standard (IS). The analytes were extracted by a liquid-liquid procedure and separated on a Zorbax Eclipse XDB C(18) column with a mobile phase composed of 2 mM aqueous ammonium acetate pH 3.2 and acetonitrile (55:45). Selected reaction monitoring was performed using the transitions m/z 435 → 415 and m/z 393 → 373 to quantify triamcinolone acetonide and betamethasone, respectively. Calibration curve was constructed over the range of 20-2000 pg/ml for triamcinolone acetonide. The lower limit of quantitation was 20 pg/ml. The mean RSD values were 4.6% and 5.7% for the intra-run and inter-run precision, respectively. The mean accuracy value was 98.5% and a recovery rate corresponding to 97.5% was achieved. No matrix effect was detected in the samples. The validated method was successfully applied to determine the plasma concentrations of triamcinolone acetonide in healthy volunteers, in a pharmacokinetic study with nasal spray formulation.
authors.affiliation: Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, 74175-100 Goiânia, GO, Brazil. isaccesar@bol.com.br
authors.forename: Isabela Costa
authors.initials: IC
authors.lastname: César
authors.forename: Ricardo Martins Duarte
authors.initials: RM
authors.lastname: Byrro
authors.forename: Fabiana Fernandes
authors.initials: FF
authors.lastname: de Santana e Silva Cardoso
authors.forename: Iram Moreira
authors.initials: IM
authors.lastname: Mundim
authors.forename: Leonardo
authors.initials: L
authors.lastname: de Souza Teixeira
authors.forename: Weidson Carlo
authors.initials: WC
authors.lastname: de Sousa
authors.forename: Sandro Antônio
authors.initials: SA
authors.lastname: Gomes
authors.forename: Karini Bruno
authors.initials: KB
authors.lastname: Bellorio
authors.forename: Juliana Machado
authors.initials: JM
authors.lastname: Brêtas
authors.forename: Gerson Antônio
authors.initials: GA
authors.lastname: Pianetti
mesh_terms: D001623:Betamethasone; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D016014:Linear Models; D059085:Nasal Sprays; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014221:Triamcinolone / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D059085:Nasal Sprays; D014221:Triamcinolone; D001623:Betamethasone