title: Exocytosis at the hair cell ribbon synapse apparently operates without neuronal SNARE proteins.
abstract: SNARE proteins mediate membrane fusion. Neurosecretion depends on neuronal soluble NSF attachment protein receptors (SNAREs; SNAP-25, syntaxin-1, and synaptobrevin-1 or synaptobrevin-2) and is blocked by neurotoxin-mediated cleavage or genetic ablation. We found that exocytosis in mouse inner hair cells (IHCs) was insensitive to neurotoxins and genetic ablation of neuronal SNAREs. mRNA, but no synaptically localized protein, of neuronal SNAREs was present in IHCs. Thus, IHC exocytosis is unconventional and may operate independently of neuronal SNAREs.
authors.affiliation: InnerEarLab, Department of Otolaryngology and Center for Molecular Physiology of the Brain, University of Göttingen Medical Center, Göttingen, Germany.
authors.forename: Régis
authors.initials: R
authors.lastname: Nouvian
authors.forename: Jakob
authors.initials: J
authors.lastname: Neef
authors.forename: Anna V
authors.initials: AV
authors.lastname: Bulankina
authors.forename: Ellen
authors.initials: E
authors.lastname: Reisinger
authors.forename: Tina
authors.initials: T
authors.lastname: Pangršič
authors.forename: Thomas
authors.initials: T
authors.lastname: Frank
authors.forename: Stefan
authors.initials: S
authors.lastname: Sikorra
authors.forename: Nils
authors.initials: N
authors.lastname: Brose
authors.forename: Thomas
authors.initials: T
authors.lastname: Binz
authors.forename: Tobias
authors.initials: T
authors.lastname: Moser
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009924:Organ Culture Techniques; D050600:SNARE Proteins / Q000172:deficiency / Q000235:genetics*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D050600:SNARE Proteins