title: Identification of recombinant intermediates of hepatitis B virus between genotype B and C in vitro.
abstract: OBJECTIVE
  To detect the recombinant intermediates of hepatitis B virus (HBV) between genotype B and C in vitro.
  
  
  METHODS
  Vector Plenti6/V5-D-topo-X was genetically modified by genotype B and C to transfect HepG2 cells. Then the HepG2 cells were amplified and sequence of the nucleic acid after the transinfection was tested and compared with RDP3Beta40 software package and bootscanning procedure in SimPlot program package.
  
  
  RESULTS
  Three recombinant intermediates of HBV between genotype B and C were identified in vitro. Genotype C in the precore region plus the core gene spanning nucleotide positions from 1740-1838 to 2443-2485 contributed to the recombination with genotype B. Isolate R1 recombinant intermediate had 2 break points at nt2170-2172 and nt2188-2189. Nucleic acid changed from CAC to TGT and from GA to AC, respectively. Isolate R2 recombinant intermediate had a break point at nt1740-1 838, and 3 bases changed in different nucleic acid sites: from A to T at nt1740, from C to T at nt1753, and from G to A at nt1838, respectively. Isolate R3 recombinant intermediate had a break point at nt2443-2483, and 4 bases changed in different nucleic acid sites: from C to T at nt2443, from A to G at nt2452, from T to C at nt2480, and from C to T at nt2483, respectively.
  
  
  CONCLUSIONS
  The recombinant intermediates of HBV between genotype B and C have been detected in vitro and the changes have been identified in the precore region plus the core gene in genotype B and C.
authors.affiliation: Department of Medicine, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China chenjunyi3@126.com
authors.forename: Junyi
authors.initials: J
authors.lastname: Chen
authors.forename: Ailong
authors.initials: A
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Dianquan
authors.initials: D
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Yu
authors.forename: Zhaojing
authors.initials: Z
authors.lastname: Zhu
authors.forename: Zuchun
authors.initials: Z
authors.lastname: Huang
authors.forename: Zongfa
authors.initials: Z
authors.lastname: Yang
authors.forename: Lishu
authors.initials: L
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Tan
mesh_terms: D001483:Base Sequence; D005822:Genetic Vectors; D005838:Genotype; D056945:Hep G2 Cells; D006512:Hepatitis B Core Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014162:Transfection
chemical_list: D006512:Hepatitis B Core Antigens