title: Aptasensor based on the selective electrodeposition of protein-linked gold nanoparticles on screen-printed electrodes.
abstract: The electrodeposition of DNA-gold nanoparticles previously developed in our group has been used as starting point for the electrodeposition of proteins attached to gold nanoparticles. We have performed a proof of principle by developing a methodology based on the electrodeposition of proteins bound to gold nanoparticles on screen-printed gold microelectrodes using, in a first approach, horseradish peroxide-conjugated gold nanoparticles (gold-HRP). The electrodeposition was achieved at a current positive potential of 800 mV vs. Ag/AgCl and the functionality of the electrodeposited HRP-particles was tested by electrochemical reduction of H(2)O(2). Furthermore, we used this proof of concept in an aptasensor application to detect Leishmania infantum KMP-11. Hence, we have demonstrated not only the functionality of the electrodeposition of proteins bound to gold nanoparticles, but also the utility of the method with the aim of developing a real biosensor containing multiple enzymes or proteins in a multimodular device.
authors.affiliation: Departamento Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Alcalá, 28871, Alcalá de Henares, Madrid, Spain. morenomm@cab.inta-csic.es
authors.forename: M
authors.initials: M
authors.lastname: Moreno
authors.forename: V M
authors.initials: VM
authors.lastname: González
authors.forename: E
authors.initials: E
authors.lastname: Rincón
authors.forename: A
authors.initials: A
authors.lastname: Domingo
authors.forename: E
authors.initials: E
authors.lastname: Domínguez
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004595:Electroplating / Q000379:methods*; D006046:Gold / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis; D018314:Leishmania infantum / Q000302:isolation & purification; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D040081:Protein Array Analysis / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; C091521:horseradish peroxidase-coupled gold; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase