title: Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure.
abstract: BACKGROUND
  Total soluble proteome alterations of white rot fungus Phanerochaete chrysosporium in response to different doses (25, 50 and 100 μM) of Pb (II) were characterized by 2DE in combination with MALDI-TOF-MS.
  
  
  RESULTS
  Dose-dependent molecular response to Pb (II) involved a total of 14 up-regulated and 21 down-regulated proteins. The induction of an isoform of glyceraldehyde 3-phosphate dehydrogenase, alcohol dehydrogenase class V, mRNA splicing factor, ATP-dependent RNA helicase, thioredoxin reductase and actin required a Pb (II) dose of at least 50 μM. Analysis of the proteome dynamics of mid-exponential phase cells of P. chrysosporium subjected to 50 μM lead at exposure time intervals of 1, 2, 4 and 8 h, identified a total of 23 proteins in increased and 67 proteins in decreased amount. Overall, the newly induced/strongly up-regulated proteins involved in (i) amelioration of lipid peroxidation products, (ii) defense against oxidative damage and redox metabolism, (iii) transcription, recombination and DNA repair (iv) a yet unknown function represented by a putative protein.
  
  
  CONCLUSIONS
  The present study implicated the particular role of the elements of DNA repair, post-tanscriptional regulation and heterotrimeric G protein signaling in response to Pb (II) stress as shown for the first time for a basidiomycete.
authors.affiliation: Department of Biological Sciences, Middle East Technical University, Ankara, Turkey. ozcengiz@metu.edu.tr.
authors.forename: Volkan
authors.initials: V
authors.lastname: Yıldırım
authors.forename: Servet
authors.initials: S
authors.lastname: Ozcan
authors.forename: Dörte
authors.initials: D
authors.lastname: Becher
authors.forename: Knut
authors.initials: K
authors.lastname: Büttner
authors.forename: Michael
authors.initials: M
authors.lastname: Hecker
authors.forename: Gülay
authors.initials: G
authors.lastname: Ozcengiz