title: Determination of penciclovir in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry: application to a clinical pharmacokinetic study.
abstract: A rapid, specific and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the determination of penciclovir in human plasma. The method involved simple, one-step SPE procedure coupled with a C(18) , 75 × 4.mm, 3 µm column with a flow-rate of 0.5 mL/min, and acyclovir was used as the internal standard. The Quattro Micro mass spectrometry was operated under the multiple reaction-monitoring mode using the electrospray ionization technique. Using 250 µL plasma, the methods were validated over the concentration range 52.555-6626.181 ng/mL, with a lower limit of quantification of 52.55 ng/mL. The intra- and inter-day precision and accuracy values were found to be within the assay variability limits as per the FDA guidelines. The developed assay method was applied to a clinical pharmacokinetic study in human volunteers.
authors.affiliation: Department of Pharmacology, LM College of Pharmacy, Gujarat University, Ahmadabad, India. drravikanneti@gmail.com
authors.forename: Ravi
authors.initials: R
authors.lastname: Kanneti
authors.forename: Dasandi
authors.initials: D
authors.lastname: Bhavesh
authors.forename: Dharmesh
authors.initials: D
authors.lastname: Paramar
authors.forename: R
authors.initials: R
authors.lastname: Shivaprakash
authors.forename: Parloop A
authors.initials: PA
authors.lastname: Bhatt
mesh_terms: D000212:Acyclovir / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D006147:Guanine; D006801:Humans; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C053539:penciclovir; D006147:Guanine; D000212:Acyclovir