title: In vivo comparison of the optical clearing efficacy of optical clearing agents in human skin by quantifying permeability using optical coherence tomography.
abstract: The objective of this work is to quantify and compare the optical clearing efficacy of glucose, propylene glycol, glycerol solutions through the human skin tissue in vivo by calculating permeability coefficient of three solutions. Currently, the permeability coefficient of agent in tissues was extracted from optical coherence tomography (OCT) amplitude data mainly through the OCT signal slope and the OCT amplitude methods. In this study, we report the OCT attenuation coefficient method which is a relatively novel and rarely reported methodology to measure the permeability coefficient during the optical skin clearing procedure. The permeability coefficients for 40% propylene glycol, glucose and glycerol were (2.74 ± 0.05) × 10(-6) cm s(-1), (1.78 ± 0.04) × 10(-6) cm s(-1) and (1.67 ± 0.04) × 10(-6) cm s(-1), respectively. It could be clearly seen that the permeability coefficient of the 40% propylene glycol solution is higher than that of 40% glucose solution, and the permeability coefficient of the 40% glucose solution is higher than that of the 40% glycerol solution. These indicate 40% propylene glycol solution is more effective than others in the human skin in vivo. We then compare and prove consistency of optical clearing efficacy figured out by three different methods.
authors.affiliation: MOE Key laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, Guangdong Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
authors.forename: Zhouyi
authors.initials: Z
authors.lastname: Guo
authors.forename: Huajiang
authors.initials: H
authors.lastname: Wei
authors.forename: Hongqin
authors.initials: H
authors.lastname: Yang
authors.forename: Yonghong
authors.initials: Y
authors.lastname: He
authors.forename: Shusen
authors.initials: S
authors.lastname: Xie
authors.forename: Guoyong
authors.initials: G
authors.lastname: Wu
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Deng
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Lanquan
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D005260:Female; D005947:Glucose / Q000493:pharmacokinetics*; D005990:Glycerol / Q000493:pharmacokinetics*; D006801:Humans; D008027:Light; D008297:Male; D055620:Optical Phenomena; D010539:Permeability; D019946:Propylene Glycol / Q000493:pharmacokinetics*; D012031:Refractometry; D012867:Skin / Q000033:anatomy & histology / Q000187:drug effects*; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D019946:Propylene Glycol; D005947:Glucose; D005990:Glycerol