title: Towards single cell heat shock response by accurate control on thermal confinement with an on-chip microwire electrode.
abstract: Metal electrodes with micron scale width enable the heating of less than a dozen cells in a confluent layer at predictable temperatures up to 85 °C with an accuracy of ±2 °C. Those performances were obtained by a preliminary robust temperature calibration based on biotin-rhodamine fluorescence and by controlling the temperature map on the substrate through thermal modeling. The temperature accuracy was proved by inducing the expression of heat shock proteins (HSP) in a few NIH-3T3 cells through a confined and precise temperature rise. Our device is therefore effective to locally induce a heat shock response with almost single-cell resolution. Furthermore, we show that cells heated at a higher temperature than the one of heat shock remain alive without producing HSP. Electrode deposition being one of the most common engineering processes, the fabrication of electrode arrays with a simple control circuit is clearly within reach for parallel testing. This should enable the study of several key mechanisms such as cell heat shock, death or signaling. In nanomedicine, controlled drug release by external stimuli such as for example temperature has attracted much attention. Our device could allow fast and efficient testing of thermoactivable drug delivery systems.
authors.affiliation: Laboratory of Integrated Micro and Mechatronics Systems/IIS UMI CNRS 2820, Institute of Industrial Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ginet
authors.forename: Kevin
authors.initials: K
authors.lastname: Montagne
authors.forename: Sho
authors.initials: S
authors.lastname: Akiyama
authors.forename: Ali
authors.initials: A
authors.lastname: Rajabpour
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Taniguchi
authors.forename: Teruo
authors.initials: T
authors.lastname: Fujii
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sakai
authors.forename: Beomjoon
authors.initials: B
authors.lastname: Kim
authors.forename: Dominique
authors.initials: D
authors.lastname: Fourmy
authors.forename: Sebastian
authors.initials: S
authors.lastname: Volz
mesh_terms: D000818:Animals; D002138:Calibration; D004563:Electrochemistry; D005898:Glass / Q000737:chemistry; D006046:Gold; D018869:Heat-Shock Response; D051379:Mice; D008839:Microelectrodes; D055616:Microtechnology / Q000295:instrumentation*; D041681:NIH 3T3 Cells; D059010:Single-Cell Analysis / Q000295:instrumentation*; D013696:Temperature
chemical_list: D006046:Gold