title: Tea contains potent inhibitors of tyrosine phosphatase PTP1B.
abstract: Tea is widely consumed all over the world. Studies have demonstrated the role of tea in prevention and treatment of various chronic diseases including diabetes and obesity, but the underlying mechanism is unclear. PTP1B is a widely expressed tyrosine phosphatase which has been defined as a target for therapeutic drug development to treat diabetes and obesity. In screening for inhibitors of PTP1B, we found that aqueous extracts of teas exhibited potent PTP1B inhibitory effects with an IC50 value of 0.4-4 g dry tea leaves per liter of water. Black tea shows the strongest inhibition activities, followed by oolong and then by green tea. Biochemical fractionations demonstrated that the major effective components in tea corresponded to oxidized polyphenolic compounds. This was further verified by the fact that tea catechins became potent inhibitors of PTP1B upon oxidation catalyzed by tyrosinases. When applied to cultured cells, tea extracts induced tyrosine phosphorylation of cellular proteins. Our study suggests that some beneficial effects of tea may be attributed to the inhibition of PTP1B.
authors.affiliation: Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Shu
authors.initials: S
authors.lastname: Xing
authors.forename: Wan-Ting Tina
authors.initials: WT
authors.lastname: Ho
authors.forename: Xueqi
authors.initials: X
authors.lastname: Fu
authors.forename: Zhizhuang Joe
authors.initials: ZJ
authors.lastname: Zhao
mesh_terms: D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology; D056148:Chromatography, Reverse-Phase; D006801:Humans; D051379:Mice; D014442:Monophenol Monooxygenase / Q000737:chemistry; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors*; D013662:Tea / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
chemical_list: D047428:Protein Kinase Inhibitors; D013662:Tea; D014443:Tyrosine; D002392:Catechin; D014442:Monophenol Monooxygenase; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1