title: Synthesis and immunogenicity of a Helicobacter pylori lipopolysaccharide-based conjugate.
abstract: Helicobacter pylori infection in humans is responsible for the onset of severe gastric disorders and a vaccine would be an improvement over current antibiotic-based treatments. Lipopolysaccharide (LPS; O-chain PS→core→lipid A) is a main H. pylori cell wall component, whose O-chain PS exhibits molecular mimicry and therefore any LPS-based vaccine cannot contain O-chain epitopes. Here, the conjugation of de-lipidated H. pylori O:2 LPS to BSA and its immunogenicity in mice is described. IgG antibodies were observed to recognize the LPSs of representative H. pylori serotypes O:1, O:2 and O:5, and more significantly, the core region of H. pylori. This study showed that a monovalent H. pylori LPS conjugate can elicit antibodies that recognize other serotype-specific H. pylori LPSs and specifically the structurally conserved LPS inner-regions.
authors.affiliation: Department of Chemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W1. monteiro@uoguelph.ca
authors.forename: Mario A
authors.initials: MA
authors.lastname: Monteiro
authors.forename: Stacey
authors.initials: S
authors.lastname: Britton
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Applebee
authors.forename: Shahida
authors.initials: S
authors.lastname: Baqar
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism; D018074:Vaccines, Conjugate / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D018074:Vaccines, Conjugate; C442865:lipopolysaccharide, Helicobacter pylori; D012710:Serum Albumin, Bovine