title: A hyaluronic acid dispersed carbon nanotube electrode used for a mediatorless NADH sensing and biosensing.
abstract: A biocompatible nanocomposite consisting of single-walled carbon nanotubes (CNTs) dispersed in a hyaluronic acid (HA) was investigated as a sensing platform for a mediatorless electrochemical detection of NADH. The device was characterised by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and extensively by electrochemistry. CNT-HA bionanocomposite showed more reversible electrochemistry, higher short-term stability of NADH sensing and higher selectivity of NADH detection compared to frequently used CNT-CHI (chitosan) modified GCE. Finally the performance of the sensor modified by CNT-HA was tested in a batch and flow injection analysis (FIA) mode of operation with basic characteristics revealed. The NADH sensor exhibits a good long-term operational stability (95% of the original sensitivity after 22 h of continuous operation). Subsequently a d-sorbitol biosensor based on such a nanoscale built interface was prepared and characterised with a d-sorbitol dehydrogenase used as a biocatalyst.
authors.affiliation: Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, SK-845 38, Slovakia.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Filip
authors.forename: Jana
authors.initials: J
authors.lastname: Sefčovičová
authors.forename: Peter
authors.initials: P
authors.lastname: Tomčík
authors.forename: Peter
authors.initials: P
authors.lastname: Gemeiner
authors.forename: Jan
authors.initials: J
authors.lastname: Tkac
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D006820:Hyaluronic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009243:NAD / Q000032:analysis*; D037742:Nanotubes, Carbon; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D037742:Nanotubes, Carbon; D009243:NAD; D006820:Hyaluronic Acid