title: The preparation of an infectious full-length cDNA clone of Saffold virus.
abstract: The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.
authors.affiliation: Department of Microbiology, Kanazawa Medical University School of Medicine, Ishikawa, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Himeda
authors.forename: Takushi
authors.initials: T
authors.lastname: Hosomi
authors.forename: Naeem
authors.initials: N
authors.lastname: Asif
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Takako
authors.initials: T
authors.lastname: Okuwa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Muraki
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Ohara
mesh_terms: D000818:Animals; D001483:Base Sequence; D017943:Cardiovirus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D018188:Cardiovirus Infections / Q000821:virology*; D002648:Child; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary