title: MUC1 expression in the oral mucosal epithelial cells of the elderly.
abstract: OBJECTIVE
  MUC1 is primarily involved in the protection of epithelial surfaces. Decreases in oral mucosal defence can be a predisposing factor for the development of oral mucosal diseases in the elderly. The aim of this study was to compare MUC1 expression level in oral mucosal epithelial cells of the elderly with that of young adults.
  
  
  METHODS
  Thirty elderly (mean age, 71.1±4.6 years) and thirty young (mean age, 26.4±2.4 years) adults (15 men and 15 women in each group) were included. Oral examination, including tooth, periodontal, and oral mucosal status, was performed and whole saliva samples were collected along with flow rate measurements. Precipitates of stimulated whole saliva were used for the evaluation of MUC1 expression using real-time PCR. Clarified supernatants were used for the measurement of pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α.
  
  
  RESULTS
  There was a significant decrease in the amounts of MUC1 transcripts in elderly subjects compared with those of young subjects, a result seen in both men (0.589-fold) and women (0.547-fold). The MUC1 expression level was not correlated with salivary cytokine level but did show a significant positive correlation with the level of periodontal inflammation (r(s)=0.505, P<0.01) in the elderly group.
  
  
  CONCLUSIONS
  Oral mucosal defence provided by MUC1 was decreased in the elderly; this decrease may play a role in the development of oral mucosal diseases in the aged population.
authors.affiliation: Dept. of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Yunkeun-Dong 28, Chongro-Ku, Seoul 110-749, Republic of Korea.
authors.forename: Woon-Ic
authors.initials: WI
authors.lastname: Chang
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Chang
authors.forename: Yoon-Young
authors.initials: YY
authors.lastname: Kim
authors.forename: Gene
authors.initials: G
authors.lastname: Lee
authors.forename: Hong-Seop
authors.initials: HS
authors.lastname: Kho
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology* / Q000378:metabolism; D004245:DMF Index; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D018396:Mucin-1 / Q000096:biosynthesis*; D010518:Periodontitis / Q000276:immunology*; D012463:Saliva / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D018396:Mucin-1; D012471:Salivary Proteins and Peptides; D014409:Tumor Necrosis Factor-alpha