title: Time course of reendothelialization of stents in a normal coronary swine model: characterization and quantification.
abstract: Late thrombosis of coronary drug-eluting stents is an infrequent but serious complication of percutaneous transluminal coronary angioplasty. The best predictor of this event is the lack of endothelialization of stent struts. The objective of this study is to characterize and quantify the time course of endothelialization of different stents implanted in nonatherosclerotic swine coronary arteries. Thirty-three Carbofilm-coated stents were implanted percutaneously in 11 anesthetized domestic, crossbred pigs (weight 25 ± 3 kg, 2 months old). Each animal received 1 stainless steel stent (SS), 1 cobalt-chromium stent (CCS), and 1 tacrolimus-eluting stent (TES) in each coronary artery. Follow-up periods were 1 day (n = 9 stents), 3 days (n = 9 stents), and 7 days (n = 15 stents). Longitudinal sections of the stented vessels were examined using scanning electron microscopy. At 1 day, there was scarce, patchy endothelialization with areas of fibrin; the endothelialization rate was similar for all the stents (SS, 29% ± 23%; CCS, 29% ± 24%; TES, 31% ± 25%; P = .9). At 3 days, there were more endothelial cells but with immature features and giant cells over fibrin; the endothelialization was greater in SS and CCS than in TES (SS, 79% ± 14%; CCS, 81% ± 17%; TES, 46% ± 9%; P = .007). At 7 days, arteries showed better endothelialization with few giant cells; the endothelialization was greater in SS and CCS than in TES (SS, 95% ± 4%; CCS, 98% ± 4%; TES, 79% ± 9%; P = .01). In conclusion, the described model is useful for the analysis of endothelialization of coronary stents and facilitates measurement of its rate of formation and characterization of the involved cell types.
authors.affiliation: HemoLeon, Grupo Cardiovascular-Fundación Investigación Sanitaria en León, Hospital de León, e Instituto de Biomedicina IBIOMED, Universidad de León, León, Spain. aperez@secardiologia.es
authors.forename: A Pérez
authors.initials: AP
authors.lastname: de Prado
authors.forename: C
authors.initials: C
authors.lastname: Pérez-Martínez
authors.forename: C
authors.initials: C
authors.lastname: Cuellas-Ramón
authors.forename: J Manuel
authors.initials: JM
authors.lastname: Gonzalo-Orden
authors.forename: M
authors.initials: M
authors.lastname: Regueiro-Purriños
authors.forename: B
authors.initials: B
authors.lastname: Martínez
authors.forename: M J
authors.initials: MJ
authors.lastname: García-Iglesias
authors.forename: J M
authors.initials: JM
authors.lastname: Ajenjo
authors.forename: J R
authors.initials: JR
authors.lastname: Altónaga
authors.forename: A
authors.initials: A
authors.lastname: Diego-Nieto
authors.forename: A
authors.initials: A
authors.lastname: de Miguel
authors.forename: F
authors.initials: F
authors.lastname: Fernández-Vázquez
mesh_terms: D015906:Angioplasty, Balloon, Coronary / Q000379:methods*; D000818:Animals; D003324:Coronary Artery Disease / Q000517:prevention & control; D023903:Coronary Restenosis / Q000517:prevention & control*; D003331:Coronary Vessels / Q000378:metabolism; D054855:Drug-Eluting Stents / Q000009:adverse effects*; D004727:Endothelium / Q000378:metabolism; D037901:Euthanasia, Animal; D005500:Follow-Up Studies; D006801:Humans; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D034421:Sus scrofa; D013552:Swine; D013927:Thrombosis / Q000517:prevention & control*; D013997:Time Factors