title: Expression of soluble TGF-β receptor II by recombinant Vaccinia virus enhances E7 specific immunotherapy of HPV16 tumors.
abstract: Therapeutic immunization with double recombinants of vaccinia virus (VACV) co-expressing sTβRII increased rejection of established TC-1 tumors in C57BL/6 mice in comparison with single recombinant expressing SigE7LAMP. Recombinant VACV derived from vaccination strain Praha expressed either the sTβRII (ectodomain) or chimeric protein fused to immunoglobulin Fc fragment (sTβRII-Fc-Jun) under control of two different promotors together with the immunogenic tumor associated antigen HPV16 E7 oncoprotein in a form of SigE7LAMP fusion molecule. The ability of soluble receptors to bind TGF-β in vitro was proved. Immunization of mice with double recombinant viruses and virus expressing SigE7LAMP only led to eliciting similar response of E7 specific CD8+ T cells as detected by IFN-γ ELISPOT.
authors.affiliation: Institute of Hematology and Blood Transfusion, Prague, U Nemocnice 1, 128 Prague Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Zurkova
authors.forename: P
authors.initials: P
authors.lastname: Chlanda
authors.forename: Z
authors.initials: Z
authors.lastname: Samkova
authors.forename: K
authors.initials: K
authors.lastname: Babiarova
authors.forename: L
authors.initials: L
authors.lastname: Kutinova
authors.forename: J
authors.initials: J
authors.lastname: Krystofova
authors.forename: P
authors.initials: P
authors.lastname: Hainz
authors.forename: S
authors.initials: S
authors.lastname: Nemeckova
mesh_terms: D000818:Animals; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000628:therapy*; D050725:Papillomavirus E7 Proteins / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000276:immunology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D050725:Papillomavirus E7 Proteins; D053918:Papillomavirus Vaccines; D018125:Receptors, Transforming Growth Factor beta; D014614:Vaccines, Synthetic; C059731:oncogene protein E7, Human papillomavirus type 16; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II