title: Inhibition of bacterial adhesion on biocompatible zwitterionic SBA-15 mesoporous materials.
abstract: In this manuscript in vitro bacterial adhesion assays using Escherichia coli on different SBA-15 nanostructured ceramics have been performed. For this purpose pure silica, NH(2) or COOH monofunctionalized, and NH(2)/COOH bifunctionalized SBA-15 mesoporous materials have been used. Material characterization reveals that both NH(2)/COOH and NH(2) functionalized SBA-15 materials exhibit a zwitterionic character due to the presence of -NH(3)(+)/COO(-) or -NH(3)(+)/SiO(-) moieties, respectively. In vitro adhesion assays have been carried out at the pH at which the zwitterionic nature of both of these samples is preserved, i.e. pH 5.5. The results show that the presence of both positive and negative moieties with an overall neutral charge leads to reduced E. coli adhesiveness. In vitro tests with cultured human Saos-2 osteoblasts have been carried out to evaluate the biocompatibility of the different materials at the physiological pH of 7.4. The results demonstrate that all materials exhibit good biocompatibility, with Saos-2 osteoblasts adhering, proliferating and maintaining their morphological and functional characteristics. This novel family of zwitterionic mesoporous materials opens up promising expectations in diverse biomedical applications, such as preventing some side-effects associated with bone implant infections.
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Izquierdo-Barba
authors.forename: Sandra
authors.initials: S
authors.lastname: Sánchez-Salcedo
authors.forename: Montserrat
authors.initials: M
authors.lastname: Colilla
authors.forename: María José
authors.initials: MJ
authors.lastname: Feito
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ramírez-Santillán
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Portolés
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D002460:Cell Line; D002516:Ceramics / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; C509969:SBA-15; D012822:Silicon Dioxide