title: Determination of tebuconazole, trifloxystrobin and its metabolite in fruit and vegetables by a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method using gas chromatography with a nitrogen-phosphorus detector and ion trap mass spectrometry.
abstract: A new analytical method using QuEChERS procedure by gas chromatography with a nitrogen-phosphorus detector (GC-NPD) and ion trap mass spectrometry (GC-IT-MS) for the quantitative determination of tebuconazole, trifloxystrobin and its metabolite trifloxystrobin acid has been developed and validated. The analytes were extracted from five fruit and vegetable matrices using acetonitrile and subsequently cleaned up using primary secondary amine (PSA) or octadecylsilane (C18) as sorbent prior to GC analysis. The present methods provided sufficient sensitivity as reflected by the values of limit of detection (LOD) and limit of quantification (LOQ) of 0.4-7 and 1.2-20 µg/kg for GC-IT-MS/MS and GC-NPD. The recoveries were, on average, 68-117 and 68-121%, respectively, for three compounds by GC-NPD and GC-IT-MS/MS with intra-day precision achieved with an RSD of 2.7-19.1%. The inter-day precision was better than 15.1% as determined by GC-NPD. The QuEChERS procedure, by using two sorbents (PSA and C18) and the matrix-matched standards, gave satisfactory recoveries and RSD values in different matrices. IT-MS acquisition provided higher specificity and selectivity for pesticides and better limit of detection and quantification. However, the repeatability and precision of NPD method were better compared with IT-MS.
authors.affiliation: Key Laboratory of Pesticide Chemistry and Application, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Wencheng
authors.initials: W
authors.lastname: Song
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000085:Acetates / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001937:Brassica / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007097:Imines / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008689:Methacrylates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000073739:Strobilurins; D053719:Tandem Mass Spectrometry; D014230:Triazoles / Q000032:analysis* / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry*; D027843:Vitis / Q000737:chemistry
chemical_list: D000085:Acetates; D007097:Imines; D008689:Methacrylates; D010573:Pesticide Residues; D000073739:Strobilurins; D014230:Triazoles; C087114:tebuconazole; C467051:trifloxystrobin