title: Improvement of cellulase production in cultures of Acremonium cellulolyticus using pretreated waste milk pack with cellulase targeting for biorefinery.
abstract: Cellulase production in cultures of Acremonium cellulolyticus was significantly improved by using waste milk pack (MP) that had been pretreated with cellulase. When MP cellulose pretreated with cellulase (3 FPU/g MP) for 12h was used as the sole carbon source for A. cellulolyticus culture in a 3-L fermentor, the cellulase activity was 16 FPU/ml. This was 25-fold higher (0.67 FPU/ml) compared with untreated MP cellulose and was comparable to that achieved with pure cellulose (Solka Floc). As the pretreatment progressed, roughness on the surface of untreated MP cellulose became to be smooth, but development of fissures on the surface of pretreated MP cellulose was observed. Cellulase pretreatment of MP increased both the accessibility of A. cellulolyticus to the surface and number of adsorption sites of cellulase on the surface of MP cellulose, leading to improved cellulase production in the A. cellulolyticus.
authors.affiliation: Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. acypark@ipc.shizuoka.ac.jp
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
authors.forename: Kazuya
authors.initials: K
authors.lastname: Naruse
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kato
mesh_terms: D000164:Acremonium / Q000378:metabolism*; D000818:Animals; D002480:Cellulase / Q000096:biosynthesis*; D008892:Milk / Q000378:metabolism*
chemical_list: D002480:Cellulase