title: Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway.
abstract: Buprofezin is a widely used insecticide that has caused environmental pollution in many areas. However, biodegradation of buprofezin by pure cultures has not been extensively studied, and the transformation pathway of buprofezin remains unclear. In this paper, a buprofezin co-metabolizing strain of DFS35-4 was isolated from a buprofezin-polluted soil in China. Strain DFS35-4 was preliminarily identified as Pseudomonas sp. based on its morphological, physiological, and biochemical properties, as well as 16S rRNA gene analysis. In the presence of 2.0 g l(-1) sodium citrate, strain DFS35-4 degraded over 70% of 50 mg l(-1) buprofezin in 3 days. Strain DFS35-4 efficiently degraded buprofezin in the pH range of 5.0-10.0 and at temperatures between 20 and 30°C. Three metabolites, 2-imino-5-phenyl-3-(propan-2-yl)-1,3,5-thiadiazinan-4-one, 2-imino-5-phenyl-1,3,5-thiadiazinan-4-one, and methyl(phenyl) carbamic acid, were identified during the degradation of buprofezin using gas chromatography-mass spectrometry (GC-MS) and tandem mass spectrometry (MS/MS). A partial transformation pathway of buprofezin in Pseudomonas sp. DFS35-4 was proposed based on these metabolites.
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Jiang
mesh_terms: D001673:Biodegradation, Environmental; D001711:Biotransformation; D002681:China; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013829:Thiadiazines / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D013829:Thiadiazines; C083997:buprofezin