title: Characterization of Plasmodium berghei glutathione synthetase.
abstract: Plasmodium berghei contained 0.454±0.031 U/mg of glutathione synthetyase (GS). GS was purified using solid ammonium sulfate and Sephadex G-200 from P. berghei infected mouse erythrocytes. SDS-PAGE showed purified GS as a single band protein of 70 kDa and its Km for γ-glutamylcysteine, glycine and ATP being 0.33 mM, 8.3 mM and 0.43 mM respectively with noncompetitive inhibition by GSH. The malaria parasite enzyme was optimally active at 37°C and pH 8.0-8.5. Heavy metals significantly inhibited parasite GS activity.
authors.affiliation: Department of Biosciences, Himachal Pradesh University, Shimla, India. sanjaysharma_bio@yahoo.com
authors.forename: S K
authors.initials: SK
authors.lastname: Sharma
authors.forename: H S
authors.initials: HS
authors.lastname: Banyal
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004151:Dipeptides / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D005978:Glutathione / Q000494:pharmacology; D005981:Glutathione Synthase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008288:Malaria / Q000469:parasitology; D019216:Metals, Heavy / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D010962:Plasmodium berghei / Q000201:enzymology*; D013347:Subcellular Fractions; D013696:Temperature
chemical_list: D004151:Dipeptides; D019216:Metals, Heavy; D000255:Adenosine Triphosphate; D005981:Glutathione Synthase; D005978:Glutathione; C017341:gamma-glutamylcysteine; D005998:Glycine