title: RP-HPLC-DAD for simultaneous estimation of mahanine and mahanimbine in Murraya koenigii.
abstract: Murraya koenigii leaves (Rutaceae) are widely used as food condiments in various food preparations in India. They possess a wide range of biological activities including antioxidant, antibacterial, anticancer, hypoglycemic and hypolipidemic activity. A rapid reverse-phase high-performance liquid chromatography (RP-HPLC) method has been developed for quantitative estimation of mahanine and mahanimbine, two major bioactive alkaloids in this plant. The amounts of mahanine and mahanimbine were detected as 9.56 ± 1.04 and 4.32 ± 0.81% w/w in the extract, with the retention times of 6.26 ± 0.66 and 10.40 ± 0.95 minutes. The limits of detection and quantification were estimated to be 29.30 and 81.12 µg/mL and 1.67 and 6.31 µg/mL, respectively. This specific and precise validated method can be useful for the routine analysis and quantitative determination of mahanine and mahanimbine in this therapeutically potent medicinal plant.
authors.affiliation: School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
authors.forename: Subrata
authors.initials: S
authors.lastname: Pandit
authors.forename: Mythies
authors.initials: M
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Ponnusankar
authors.forename: B C
authors.initials: BC
authors.lastname: Pal
authors.forename: Pulok K
authors.initials: PK
authors.lastname: Mukherjee
mesh_terms: D002227:Carbazoles / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods; D006576:Heterocyclic Compounds, 4 or More Rings / Q000032:analysis*; D032101:Murraya / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D002227:Carbazoles; D006576:Heterocyclic Compounds, 4 or More Rings; D010936:Plant Extracts; C556937:mahanimbine; C465290:mahanine