title: Structure of Bombyx mori densovirus 1, a silkworm pathogen.
abstract: Bombyx mori densovirus 1 (BmDNV-1), a major pathogen of silkworms, causes significant losses to the silk industry. The structure of the recombinant BmDNV-1 virus-like particle has been determined at 3.1-Å resolution using X-ray crystallography. It is the first near-atomic-resolution structure of a virus-like particle within the genus Iteravirus. The particles consist of 60 copies of the 55-kDa VP3 coat protein. The capsid protein has a β-barrel "jelly roll" fold similar to that found in many diverse icosahedral viruses, including archaeal, bacterial, plant, and animal viruses, as well as other parvoviruses. Most of the surface loops have little structural resemblance to other known parvovirus capsid proteins. In contrast to vertebrate parvoviruses, the N-terminal β-strand of BmDNV-1 VP3 is positioned relative to the neighboring 2-fold related subunit in a "domain-swapped" conformation, similar to findings for other invertebrate parvoviruses, suggesting domain swapping is an evolutionarily conserved structural feature of the Densovirinae.
authors.affiliation: Department of Biological Sciences, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907-2032, USA.
authors.forename: Bärbel
authors.initials: B
authors.lastname: Kaufmann
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Far
authors.forename: Pavel
authors.initials: P
authors.lastname: Plevka
authors.forename: Valorie D
authors.initials: VD
authors.lastname: Bowman
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Peter
authors.initials: P
authors.lastname: Tijssen
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rossmann
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D018360:Crystallography, X-Ray; D019223:Densovirinae / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D022701:Virosomes / Q000737:chemistry
chemical_list: D046911:Macromolecular Substances; D022701:Virosomes