title: Small-molecule probe using dual signals to monitor leucine aminopeptidase activity.
abstract: Leucine aminopeptidases (LAPs) are widely distributed in organisms from bacteria to humans, and play crucial roles in cell maintenance and cell growth. Thus, assays for LAP are necessary for measuring its activity and inhibitor potency. In this Letter, we report a small-molecule probe which exhibits colorimetric and fluorogenic changes according to LAP activity.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Hey Young
authors.initials: HY
authors.lastname: Yoon
authors.forename: So Hee
authors.initials: SH
authors.lastname: Shim
authors.forename: Luck Ju
authors.initials: LJ
authors.lastname: Baek
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D002151:Calorimetry / Q000379:methods*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D005663:Furans / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D007931:Leucyl Aminopeptidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000737:chemistry / Q000494:pharmacology; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D005663:Furans; D009570:Nitriles; D011480:Protease Inhibitors; D054852:Small Molecule Libraries; C521333:dicyanomethylenedihydrofuran; D007931:Leucyl Aminopeptidase