title: Feeder-dependent and feeder-independent iPS cell derivation from human and mouse adipose stem cells.
abstract: Adipose tissue is an abundantly available source of proliferative and multipotent mesenchymal stem cells with promising potential for regenerative therapeutics. We previously demonstrated that both human and mouse adipose-derived stem cells (ASCs) can be reprogrammed into induced pluripotent stem cells (iPSCs) with efficiencies higher than those that have been reported for other cell types. The ASC-derived iPSCs can be generated in a feeder-independent manner, representing a unique model to study reprogramming and an important step toward establishing a safe, clinical grade of cells for therapeutic use. In this study, we provide a detailed protocol for isolation, preparation and transformation of ASCs from fat tissue into mouse iPSCs in feeder-free conditions and human iPSCs using feeder-dependent or feeder/xenobiotic-free processes. This protocol also describes how ASCs can be used as feeder cells for maintenance of other pluripotent stem cells. ASC derivation is rapid and can be completed in <1 week, with mouse and human iPS reprogramming times averaging 1.5 and 2.5 weeks, respectively.
authors.affiliation: Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sugii
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kida
authors.forename: W Travis
authors.initials: WT
authors.lastname: Berggren
authors.forename: Ronald M
authors.initials: RM
authors.lastname: Evans
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D018920:Coculture Techniques / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D039904:Pluripotent Stem Cells / Q000378:metabolism; D013997:Time Factors