title: Overview of the generation, validation, and application of phosphosite-specific antibodies.
abstract: Protein phosphorylation is a universal key posttranslational modification that affects the activity and other properties of intracellular proteins. Phosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is key for their use in proteomics and profiling of disease.
authors.affiliation: R&D Systems, Inc, Minneapolis, MN, USA.
authors.forename: Kathy
authors.initials: K
authors.lastname: Brumbaugh
authors.forename: Wade
authors.initials: W
authors.lastname: Johnson
authors.forename: Wen-Chieh
authors.initials: WC
authors.lastname: Liao
authors.forename: Mong-Shang
authors.initials: MS
authors.lastname: Lin
authors.forename: J P
authors.initials: JP
authors.lastname: Houchins
authors.forename: Jeff
authors.initials: J
authors.lastname: Cooper
authors.forename: Steven
authors.initials: S
authors.lastname: Stoesz
authors.forename: Roberto
authors.initials: R
authors.lastname: Campos-Gonzalez
mesh_terms: D000818:Animals; D045163:Antibodies, Phospho-Specific / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D055808:Drug Discovery / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D054928:Validation Studies as Topic
chemical_list: D045163:Antibodies, Phospho-Specific; D011506:Proteins