title: Canoe functions at the CNS midline glia in a complex with Shotgun and Wrapper-Nrx-IV during neuron-glia interactions.
abstract: Vertebrates and insects alike use glial cells as intermediate targets to guide growing axons. Similar to vertebrate oligodendrocytes, Drosophila midline glia ensheath and separate axonal commissures. Neuron-glia interactions are crucial during these events, although the proteins involved remain largely unknown. Here, we show that Canoe (Cno), the Drosophila ortholog of AF-6, and the DE-cadherin Shotgun (Shg) are highly restricted to the interface between midline glia and commissural axons. cno mutant analysis, genetic interactions and co-immunoprecipitation assays unveil Cno function as a novel regulator of neuron-glia interactions, forming a complex with Shg, Wrapper and Neurexin IV, the homolog of vertebrate Caspr/paranodin. Our results also support additional functions of Cno, independent of adherens junctions, as a regulator of adhesion and signaling events in non-epithelial tissues.
authors.affiliation: Instituto de Neurociencias de Alicante, CSIC/UMH, Sant Joan d'Alacant, Alicante, Spain.
authors.forename: Jana
authors.initials: J
authors.lastname: Slováková
authors.forename: Ana
authors.initials: A
authors.lastname: Carmena
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D015820:Cadherins; D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; C491321:Nrx protein, Drosophila; C092747:cno protein, Drosophila; C501889:shg protein, Drosophila; C116202:wrapper protein, Drosophila