title: [Quick determination of 39 hormones residues in infant formula by liquid chromatography-tandem mass spectrometry].
abstract: A quick confirmative method was developed for determining the residues of 17 glucocorticoids, 11 progesterones, 3 androgens and 8 estrogens in infant formula by liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS). The sample was extracted with acetonitrile at first. Then the lipid substances were removed by centrifugation under freezing condition and liquid-liquid extraction with hexane of the extract. The purification was carried out on hydrophilic-lipophilic solid-phase extraction columns and methanol was used as the eluted solvent. The detection of 39 analytes was carried out in the positive or negative multi-reaction monitoring (MRM) mode, separately. Acetonitrile-0.1% formic acid was used as the mobile phase and an ordinary silica gel C18 column was selected to separate the analytes in the positive mode. Acetonitrile-0.1% aqueous ammonia as mobile phase and the separation was carried out on an ultra-performance C18 column with a wide pH range in the negative mode. The limits of quantification (LOQ, S/N > or = 10) were 0.02-5 microg/kg. The overall recoveries varied from 59.5% to 117.9%, and the relative standard deviations (RSD) were between 6.4% and 16.3%. The real sample tests showed that the simple and accurate method can be used for determining the residues of multi-endogenous and chemically synthesized hormones in milk powders.
authors.affiliation: Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China. wxzhu121@163.com
authors.forename: Weixia
authors.initials: W
authors.lastname: Zhu
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
authors.forename: Jizhou
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D004967:Estrogens / Q000032:analysis; D005938:Glucocorticoids / Q000032:analysis; D006728:Hormones / Q000032:analysis*; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry*; D008892:Milk / Q000737:chemistry; D011374:Progesterone / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004967:Estrogens; D005938:Glucocorticoids; D006728:Hormones; D011374:Progesterone