title: Dual activity of aminoarylthiazoles on the trafficking and gating defects of the cystic fibrosis transmembrane conductance regulator chloride channel caused by cystic fibrosis mutations.
abstract: A large fraction of mutations causing cystic fibrosis impair the function of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel by causing reduced channel activity (gating defect) and/or impaired exit from the endoplasmic reticulum (trafficking defect). Such defects need to be treated with separate pharmacological compounds termed potentiators and correctors, respectively. Here, we report the characterization of aminoarylthiazoles (AATs) as compounds having dual activity. Cells expressing mutant CFTR were studied with functional assays (fluorescence-based halide transport and short circuit current measurements) to assess the effect of acute and chronic treatment with compounds. We found that AATs are effective on F508del, the most frequent cystic fibrosis mutation, which is associated with both a gating and a trafficking defect. AATs are also effective on mutations like G1349D and G551D, which cause only a gating defect. Evaluation of a panel of AAT analogs identified EN277I as the most effective compound. Incubation of cells expressing mutant CFTR with EN277I caused a strong stimulation of channel activity as demonstrated by single channel recordings. Compounds with dual activity such as AATs may be useful for the development of effective drugs for the treatment of cystic fibrosis.
authors.affiliation: Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Pedemonte
authors.forename: Valeria
authors.initials: V
authors.lastname: Tomati
authors.forename: Elvira
authors.initials: E
authors.lastname: Sondo
authors.forename: Emanuela
authors.initials: E
authors.lastname: Caci
authors.forename: Enrico
authors.initials: E
authors.lastname: Millo
authors.forename: Andrea
authors.initials: A
authors.lastname: Armirotti
authors.forename: Gianluca
authors.initials: G
authors.lastname: Damonte
authors.forename: Olga
authors.initials: O
authors.lastname: Zegarra-Moran
authors.forename: Luis J V
authors.initials: LJ
authors.lastname: Galietta
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D003550:Cystic Fibrosis / Q000235:genetics*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D015640:Ion Channel Gating / Q000235:genetics; D009154:Mutation; D018408:Patch-Clamp Techniques; D013329:Structure-Activity Relationship; D013844:Thiazoles / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D013844:Thiazoles; D019005:Cystic Fibrosis Transmembrane Conductance Regulator