title: Biobanking human endometrial tissue and blood specimens: standard operating procedure and importance to reproductive biology research and diagnostic development.
abstract: OBJECTIVE
  To develop a standard operating procedure (SOP) for collection, transport, storage of human endometrial tissue and blood samples, subject and specimen annotation, and establishing sample priorities.
  
  
  METHODS
  The SOP synthesizes sound scientific procedures, the literature on ischemia research, sample collection and gene expression profiling, good laboratory practices, and the authors' experience of workflow and sample quality.
  
  
  METHODS
  The National Institutes of Health, University of California, San Francisco, Human Endometrial Tissue and DNA Bank.
  
  
  METHODS
  Women undergoing endometrial biopsy or hysterectomy for nonmalignant indications.
  
  
  METHODS
  Collecting, processing, storing, distributing endometrial tissue and blood samples under approved institutional review board protocols and written informed consent from participating subjects.
  
  
  METHODS
  Standard operating procedure.
  
  
  RESULTS
  The SOP addresses rigorous and consistent subject annotation, specimen processing and characterization, strict regulatory compliance, and a reference for researchers to track collection and storage times that may influence their research.
  
  
  CONCLUSIONS
  The comprehensive and systematic approach to the procurement of human blood and endometrial tissue in this SOP ensures the high quality, reliability, and scientific usefulness of biospecimens made available to investigators by the National Institutes of Health, University of California, San Francisco, Human Endometrial Tissue and DNA Bank. The detail and perspective in this SOP also provides a blueprint for implementation of similar collection programs at other institutions.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sheldon
authors.forename: Kim Chi
authors.initials: KC
authors.lastname: Vo
authors.forename: Ramsey A
authors.initials: RA
authors.lastname: McIntire
authors.forename: Lusine
authors.initials: L
authors.lastname: Aghajanova
authors.forename: Zara
authors.initials: Z
authors.lastname: Zelenko
authors.forename: Juan C
authors.initials: JC
authors.lastname: Irwin
authors.forename: Linda C
authors.initials: LC
authors.lastname: Giudice
mesh_terms: D001695:Biology / Q000379:methods / Q000592:standards; D035843:Biomedical Research / Q000379:methods* / Q000592:standards; D001706:Biopsy / Q000379:methods / Q000592:standards; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards*; D003944:Diagnostic Techniques, Obstetrical and Gynecological / Q000592:standards*; D004717:Endometrium / Q000473:pathology / Q000601:surgery*; D005260:Female; D013509:Gynecologic Surgical Procedures / Q000379:methods / Q000592:standards*; D006801:Humans; D011356:Product Labeling / Q000592:standards; D011786:Quality Control; D012015:Reference Standards; D012098:Reproduction / Q000502:physiology; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D014015:Tissue Banks / Q000592:standards*