title: Photobleaching-activated micropatterning on self-assembled monolayers.
abstract: Functional chemical micropatterns were fabricated by exploiting the photobleaching of dye-coupled species near methacrylate self-assembled monolayers. Using this approach we have demonstrated that multiple chemistries can be coupled to the monolayer using a standard fluorescence microscope. The surface bound functional groups remain active and patterns with feature sizes down to 3 µm can be readily achieved with excellent signal-to-noise ratio. Control over the ligand binding density was demonstrated to illustrate the convenient route provided by this platform for fabricating complex spatial gradients in ligand density.
authors.affiliation: School of Physics and Petit Institute for Bioengineering and Biosciences (IBB), Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Scrimgeour
authors.forename: Vamsi K
authors.initials: VK
authors.lastname: Kodali
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Kovari
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Curtis
mesh_terms: D008027:Light; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry* / Q000528:radiation effects*; D038761:Photobleaching; D013499:Surface Properties
chemical_list: D008689:Methacrylates