title: An NK and T cell enhancer lies 280 kilobase pairs 3' to the gata3 structural gene.
abstract: Transcription factor GATA-3 is vital for multiple stages of T cell and natural killer (NK) cell development, and yet the factors that directly regulate Gata3 transcription during hematopoiesis are only marginally defined. Here, we show that neither of the Gata3 promoters, previously implicated in its tissue-specific regulation, is alone capable of directing Gata3 transcription in T lymphocytes. In contrast, by surveying large swaths of DNA surrounding the Gata3 locus, we located a cis element that can recapitulate aspects of the Gata3-dependent T cell regulatory program in vivo. This element, located 280 kbp 3' to the structural gene, directs both T cell- and NK cell-specific transcription in vivo but harbors no other tissue activity. This novel, distant element regulates multiple major developmental stages that require GATA-3 activity.
authors.affiliation: Department of Cell and Developmental Biology, 3071 BSRB, 109 Zina Pitcher Place, University of Michigan Medical School, Ann Arbor, MI 48103, USA.
authors.forename: Sakie
authors.initials: S
authors.lastname: Hosoya-Ohmura
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Lin
authors.forename: Mary
authors.initials: M
authors.lastname: Herrmann
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuroha
authors.forename: Arvind
authors.initials: A
authors.lastname: Rao
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriguchi
authors.forename: Kim-Chew
authors.initials: KC
authors.lastname: Lim
authors.forename: Tomonori
authors.initials: T
authors.lastname: Hosoya
authors.forename: James Douglas
authors.initials: JD
authors.lastname: Engel
mesh_terms: D000818:Animals; D004742:Enhancer Elements, Genetic; D050990:GATA3 Transcription Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism*; D019891:Leukopoiesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D050990:GATA3 Transcription Factor