title: Transcriptional pattern of TGF-beta1 inhibitory effect on mouse C2C12 myoblasts differentiation.
abstract: The aim of the present study was to define the effect of TGF-beta1 on C2C12 myoblasts myogenesis. TGF-beta1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-beta1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-beta1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-beta1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-beta1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-beta1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Bax, MdfL, Tef, Tubg1, Cxcl5, Rho, Calca and Lgals4.
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Wicik
authors.forename: T
authors.initials: T
authors.lastname: Sadkowski
authors.forename: M
authors.initials: M
authors.lastname: Jank
authors.forename: T
authors.initials: T
authors.lastname: Motyl
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D024510:Muscle Development / Q000502:physiology; D032446:Myoblasts / Q000166:cytology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D053773:Transforming Growth Factor beta1