title: Regulation of cancer cell proliferation by caveolin-2 down-regulation and re-expression.
abstract: We investigated whether altering caveolin-2 (cav-2) expression affects the proliferation of cancer cells. Cav-2 was not detected in HepG2, SH-SY5Y and LN-CaP cells, and the loss of cav-2 expression was not restored by 5-aza-2'-deoxycytidine treatment. In contrast, C6, HeLa, A549, MCF7 and PC3M cells expressed cav-2. Effects of re-expression of exogenous cav-2 in HepG2, SH-SY5Y and LN-CaP cells, and siRNA-mediated down-regulation of endogenous cav-2 in C6, HeLa, A549, MCF7 and PC3M cells on cancer proliferation were examined by MTT assay, colony formation assay and flow cytometric analysis. Cav-2 transfection in HepG2 hepatocellular carcinoma cells and knockdown in C6 glioma cells caused reduction in cell proliferation and growth with retarded entry into the S phase. Cav-2 re-expression in SH-SY5Y neuroblastoma cells and depletion in HeLa epithelial cervical cancer and A549 lung adenocarcinoma cells promoted cancer cell proliferation. Luciferase reporter assay showed that transcriptional activation of Elk-1 and STAT3 was significantly decreased in cav-2-transfected HepG2 hepatocellular carcinoma and down-regulated C6 glioma cells. Our data suggest that cav-2 acts as a modulator of cancer progression.
authors.affiliation: Department of Biochemistry, Division of Applied Life Science (BK21), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Sangho
authors.initials: S
authors.lastname: Lee
authors.forename: Hayeong
authors.initials: H
authors.lastname: Kwon
authors.forename: Kyuho
authors.initials: K
authors.lastname: Jeong
authors.forename: Yunbae
authors.initials: Y
authors.lastname: Pak
mesh_terms: D000818:Animals; D051243:Caveolin 2 / Q000037:antagonists & inhibitors / Q000502:physiology*; D049109:Cell Proliferation; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D005910:Glioma / Q000473:pathology; D056945:Hep G2 Cells; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D009447:Neuroblastoma / Q000473:pathology; D051381:Rats; D050796:STAT3 Transcription Factor / Q000502:physiology; D050807:ets-Domain Protein Elk-1 / Q000502:physiology
chemical_list: D051243:Caveolin 2; D050796:STAT3 Transcription Factor; D050807:ets-Domain Protein Elk-1; D048049:Extracellular Signal-Regulated MAP Kinases