title: Detection of cytotoxic activity of lectin on human colon adenocarcinoma (Sw480) and epithelial cervical carcinoma (C33-A).
abstract: Lectins comprise a heterogeneous class of proteins that recognize the carbohydrate moieties of glycoconjugates with high specificity. Numerous studies have shown that lectins are capable of recognizing specific carbohydrate moieties displayed by malignant cells or tissues. The present work was performed to investigate the effects of tepary bean (Phaseolus acutifolius) lectins on proliferation, colony formation, and alteration of DNA synthesis of human malignant cells. Tepary bean lectin showed dose dependent  effects on the inhibition of viability as well as on colony formation in two human malignant cells lines (C33-A, Sw480); By contrast, tepary bean lectin only showed significant effects on DNA synthesis on Sw480 cells. Our results provide evidence of the anti- proliferative and cytotoxic effects of the tepary bean lectins on C33-A and Sw480 cells lines.
authors.affiliation: Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, 42080 Pachuca de Soto, Hgo, Mexico. m.valadezvega@lycos.com
authors.forename: Carmen
authors.initials: C
authors.lastname: Valadez-Vega
authors.forename: Gerardo
authors.initials: G
authors.lastname: Alvarez-Manilla
authors.forename: Leticia
authors.initials: L
authors.lastname: Riverón-Negrete
authors.forename: Alejandro
authors.initials: A
authors.lastname: García-Carrancá
authors.forename: José A
authors.initials: JA
authors.lastname: Morales-González
authors.forename: Clara
authors.initials: C
authors.lastname: Zuñiga-Pérez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Madrigal-Santillán
authors.forename: Jaime
authors.initials: J
authors.lastname: Esquivel-Soto
authors.forename: Cesar
authors.initials: C
authors.lastname: Esquivel-Chirino
authors.forename: Roberto
authors.initials: R
authors.lastname: Villagómez-Ibarra
authors.forename: Mirandeli
authors.initials: M
authors.lastname: Bautista
authors.forename: Angel
authors.initials: A
authors.lastname: Morales-González
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
chemical_list: D037102:Lectins