title: Calcium phosphate combination biomaterials as human mesenchymal stem cell delivery vehicles for bone repair.
abstract: A new class of biomimetic, bioresorbable apatitic calcium phosphate cement (CPC) was recently developed. The handling characteristics, and the ability to harden at body temperature in the presence of physiological saline, make this material an attractive clinical bone substitute and delivery vehicle for therapeutic agents in orthopedic applications. The major challenge with the material is formulating an injectable paste with options for cell delivery, in order to regenerate new bone faster and with high quality. In this study, three different additives and/or viscosity modifiers (carboxymethylcellulose, silk, and alginate) were incorporated into a CPC matrix. Injectability, cell viability, cell proliferation, surface morphology, and gene expression for osteogenesis of hMSCs were all evaluated. Injectable CPC-gel composites with cell protection were achieved. The CPC modified with alginate provided the best results based on cell proliferation, ALP and collagen production, and osteogenic transcript increases (for ALP, type I collagen, BSP, and OP). Furthermore, osteogenic analysis indicated lineage-specific differentiation of hMSCs into osteogenic outcomes. The results suggest that CPC mixed with alginate can be used as a cell delivery vehicle for bone regeneration.
authors.affiliation: ETEX Corporation, University Park at MIT, Cambridge, Massachusetts 02139, USA.
authors.forename: Sang-Hyug
authors.initials: SH
authors.lastname: Park
authors.forename: Aliassghar
authors.initials: A
authors.lastname: Tofighi
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.forename: Michael
authors.initials: M
authors.lastname: Strunk
authors.forename: Thomas
authors.initials: T
authors.lastname: Ricketts
authors.forename: Jerry
authors.initials: J
authors.lastname: Chang
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001843:Bone Cements / Q000737:chemistry; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D001843:Bone Cements; D018786:Bone Substitutes; D002130:Calcium Phosphates