title: Establishment and characterization of primary lung cancer cell lines from Chinese population.
abstract: OBJECTIVE
  To establish and characterize primary lung cancer cell lines from Chinese population.
  
  
  METHODS
  Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice.
  
  
  RESULTS
  Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations.
  
  
  CONCLUSIONS
  These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.forename: Yi-hua
authors.initials: YH
authors.lastname: Sun
authors.forename: Xiao-lei
authors.initials: XL
authors.lastname: Ye
authors.forename: Hai-quan
authors.initials: HQ
authors.lastname: Chen
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Ji
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D044466:Asian People; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009154:Mutation; D009368:Neoplasm Transplantation
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors