title: Acinetobacter oleivorans sp. nov. is capable of adhering to and growing on diesel-oil.
abstract: A diesel-oil and n-hexadecane-degrading novel bacterial strain, designated DR1(T), was isolated from a rice paddy in Deok-So, South Korea. The strain DR1(T) cells were Gram-negative, aerobic coccobacilli, and grew at 20-37°C with the optimal temperature of 30°C, and an optimal pH of 6-8. Interestingly, strain DR1(T) was highly motile (swimming and swarming motility) using its fimbriae, and generated N-acyl homoserine lactones as quorum-sensing signals. The predominant respiratory quinone as identified as ubiquinone-9 (Q-9) and DNA G+C content was 41.4 mol%. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species A. calcoaceticus, A. haemolyticus, A. baumannii, A. baylyi, and A. beijerinckii, with which it evidenced sequence similarities of 98.2%, 97.4%, 97.2%, 97.1%, and 97.0%, respectively. DNA-DNA hybridization values between strain DR1(T) and other Acinetobacter spp. were all less than 20%. The physiological and taxonomic characteristics with the DNA-DNA hybridization data supported the identification of strain DR1(T) in the genus Acinetobacter as a novel species, for which the name Acinetobacter oleivorans sp. nov. is proposed. The type strain is DR1(T) (=KCTC 23045(T) =JCM 16667(T)).
authors.affiliation: Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-713, Republic of Korea.
authors.forename: Yoon-Suk
authors.initials: YS
authors.lastname: Kang
authors.forename: Jaejoon
authors.initials: J
authors.lastname: Jung
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
authors.forename: Woojun
authors.initials: W
authors.lastname: Park
mesh_terms: D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D000473:Alkanes / Q000378:metabolism; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005742:Gasoline / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000254:growth & development; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D000473:Alkanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005742:Gasoline; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C007932:n-hexadecane