title: Colonic availability of polyphenols and D-(-)-quinic acid after apple smoothie consumption.
abstract: METHODS
  The aim of this study was to determine the amounts of polyphenols and D-(-)-quinic acid reaching the ileostomy bags of probands (and thus the colon in healthy humans) after ingestion of apple smoothie, a beverage containing 60% cloudy apple juice and 40% apple puree.
  
  
  RESULTS
  Ten healthy ileostomy subjects each ingested 0.7 L of apple smoothie (a bottle). Their ileostomy bags were collected directly before and 1, 2, 4, 6 and 8 h after smoothie consumption, and the polyphenol and D-(-)-quinic acid contents of the ileostomy fluids were examined using HPLC-DAD and HPLC-MS/MS. The total polyphenol and D-(-)-quinic acid content of the apple smoothie was determined to be 1955.6±124.6 mg/0.7 L, which is very high compared to cloudy apple juices. The most abundant substances found in the ileostomy bags were oligomeric procyanidins (705.6±197.9 mg), D-(-)-quinic acid (363.4±235.5 mg) and 5-caffeoylquinic acid (76.7±26.8 mg). Overall recovery of ingested polyphenols and D-(-)-quinic acid in the ileostomy bags was 63.3±16.1%.
  
  
  CONCLUSIONS
  The amounts of polyphenol and D-(-)-quinic acids reaching the ileostomy bags are considerably higher after apple smoothie consumption than after the consumption of cloudy apple juice or cider. These results suggest that the food matrix might affect the colonic availability of polyphenols, and apple smoothies could be more effective in the prevention of chronic colon diseases than both cloudy apple juice and apple cider.
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Hagl
authors.forename: Hannah
authors.initials: H
authors.lastname: Deusser
authors.forename: Buelent
authors.initials: B
authors.lastname: Soyalan
authors.forename: Christine
authors.initials: C
authors.lastname: Janzowski
authors.forename: Frank
authors.initials: F
authors.lastname: Will
authors.forename: Helmut
authors.initials: H
authors.lastname: Dietrich
authors.forename: Franz Werner
authors.initials: FW
authors.lastname: Albert
authors.forename: Simone
authors.initials: S
authors.lastname: Rohner
authors.forename: Elke
authors.initials: E
authors.lastname: Richling
mesh_terms: D000328:Adult; D000368:Aged; D001628:Beverages; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005419:Flavonoids / Q000032:analysis*; D006801:Humans; D007081:Ileostomy; D008297:Male; D027845:Malus / Q000737:chemistry*; D008875:Middle Aged; D010636:Phenols / Q000032:analysis*; D010693:Phloretin / Q000032:analysis; D059808:Polyphenols; D044945:Proanthocyanidins / Q000032:analysis; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis*
chemical_list: D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D044945:Proanthocyanidins; C472707:caffeoylquinic acid; D011801:Quinic Acid; D010693:Phloretin