title: Autologous periodontal ligament cells in the treatment of class II furcation defects: a study in dogs.
abstract: OBJECTIVE
  The goal of this study was to histologically investigate the use of periodontal ligament cells (PDL cells) in tissue engineering to regenerate class II furcation defects.
  
  
  METHODS
  PDL cells were obtained from the mandibular tooth extracted from each dog (seven), cultured in vitro and phenotypically characterized with regard to their biological properties. Following, bilateral class II furcation lesions were created at maxillary 3rd premolars and were randomly assigned to the test group [PDL cells+guided tissue regeneration (GTR)] or the control group (GTR). After 3 months, the animals were euthanized to evaluate the histometric parameters.
  
  
  RESULTS
  In vitro, PDL cells were able to promote mineral nodule formation and to express bone sialoprotein, type I collagen and alkaline phosphatase. Histometrically, data analysis demonstrated that the cell-treated group presented a superior length of new cementum (6.00 ± 1.50 and 8.08 ± 1.08 mm), a greater extension of periodontal regeneration (3.94 ± 1.20 and 7.28 ± 1.00 mm), a lower formation of connective tissue/epithelium (2.15 ± 1.92 and 0.60 ± 0.99 mm), a larger area of new bone (7.01 ± 0.61 and 9.02 ± 2.30 mm(2)) and a smaller area of connective tissue/epithelium (5.90 ± 1.67 and 4.22 ± 0.95 mm(2)), when compared with control group.
  
  
  CONCLUSIONS
  PDL cells in association with GTR may significantly promote periodontal regeneration in class II furcation defects in dog.
authors.affiliation: Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, State University of Campinas, São Paulo, Brazil.
authors.forename: Fabricia Ferreira
authors.initials: FF
authors.lastname: Suaid
authors.forename: Fernanda Vieira
authors.initials: FV
authors.lastname: Ribeiro
authors.forename: Thaisângela L
authors.initials: TL
authors.lastname: Rodrigues
authors.forename: Karina Gonzales
authors.initials: KG
authors.lastname: Silvério
authors.forename: Marcelo Diniz
authors.initials: MD
authors.lastname: Carvalho
authors.forename: Francisco Humberto
authors.initials: FH
authors.lastname: Nociti
authors.forename: Marcio Zaffalon
authors.initials: MZ
authors.lastname: Casati
authors.forename: Enilson Antônio
authors.initials: EA
authors.lastname: Sallum
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D004285:Dogs; D017823:Furcation Defects / Q000628:therapy*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D010012:Osteogenesis / Q000502:physiology*; D010513:Periodontal Ligament / Q000166:cytology*; D011897:Random Allocation; D016037:Single-Blind Method; D023822:Tissue Engineering; D054457:Tissue Scaffolds