title: Poly(ADP-ribosyl)ation of mannose-binding lectin out of human kidney cells.
abstract: Mannose-binding lectin was identified as a substrate of tankyrase 2, an enzyme that catalyzes poly(ADP-ribosyl)ation. The endogenous tankyrase 2 was isolated out of cytoplasm of human embryonic kidney cells. It was bound to a soluble complex of at least two other proteins; they were identified using specific antibodies and other approaches as keratin 1 and mannose-binding lectin. Using immunoblot analysis and radioactive labeling, we detected tankyrase-2-dependent poly(ADP-ribosyl)ation of mannose-binding lectin. In the presence of NAD(+), the complex of keratin 1 and lectin was dissociated, what was recorded during elution of its separate components out of affinity columns and by decrease of their apparent molecular masses during gel-filtration. Tankyrase 2 also inhibited the carbohydrate-binding function of the lectin. The latter effect was observed using mannose-binding lectin out of human serum, which is free from keratin 1. As a result of tankyrase-2 activity, the lectin lost its affinity to mannan-agarose. The discovery of this new biochemical mechanism justifies further analysis of its physiological and medical significance.
authors.affiliation: A N Belozersky Institute, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Natalie N
authors.initials: NN
authors.lastname: Sidorova
authors.forename: Svetlana Yu
authors.initials: SY
authors.lastname: Kurchashova
authors.forename: Tural Ya
authors.initials: TY
authors.lastname: Yarahmedov
authors.forename: Rustam H
authors.initials: RH
authors.lastname: Ziganshin
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Kuimov
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D013058:Mass Spectrometry; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*
chemical_list: D037601:Mannose-Binding Lectin; D011064:Poly Adenosine Diphosphate Ribose