title: Soluble CD30 and Hepatocyte growth factor as predictive markers of antibody-mediated rejection of the kidney allograft.
abstract: Our retrospective study was aimed to assess the relevance of pre- and post-transplant measurements of serum concentrations of the soluble CD30 molecule (soluble CD30, sCD30) and the cytokine Hepatocyte growth factor (HGF) for prediction of the risk for development of antibody-mediated rejection (AMR) in kidney transplant patients. Evaluation of sCD30, HGF levels and the presence of HLA-specific antibodies in a cohort of 205 patients was performed before, 2weeks and 6months after transplantation. Patients were followed up for kidney graft function and survival for two years. We found a tendency of higher incidence of AMR in retransplanted patients with elevated pre-transplant sCD30 (≥100U/ml) (p=0.051), however no such correlation was observed in first-transplant patients. Kidney recipients with simultaneously high sCD30 and HLA-specific antibodies (sCD30+/Ab+) before transplantation had significantly lower AMR-free survival compared to the other patient groups (p<0.001). HGF concentrations were not associated with the incidence of AMR at any time point of measurement, nevertheless, the combined analysis HGF and sCD30 showed increased incidence of AMR in recipients with elevated pretransplant sCD30 and low HGF levels.
  
  
  CONCLUSIONS
  the predictive value of pretransplant sCD30 for the development of antibody-mediated rejection after transplantation is significantly potentiated by the co-presence of HLA specific antibodies. The role of HGF as a rejection-protective factor in patients with high pretransplant HGF levels would need further investigation.
authors.affiliation: Department of Immunogenetics, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 140 21 Prague, Czech Republic.
authors.forename: Yelena
authors.initials: Y
authors.lastname: Pavlova
authors.forename: Ondrej
authors.initials: O
authors.lastname: Viklicky
authors.forename: Janka
authors.initials: J
authors.lastname: Slatinska
authors.forename: Marcela
authors.initials: M
authors.lastname: Bürgelova
authors.forename: Caner
authors.initials: C
authors.lastname: Süsal
authors.forename: Jelena
authors.initials: J
authors.lastname: Skibova
authors.forename: Eva
authors.initials: E
authors.lastname: Honsová
authors.forename: Ilja
authors.initials: I
authors.lastname: Striz
authors.forename: Libor
authors.initials: L
authors.lastname: Kolesar
authors.forename: Antonij
authors.initials: A
authors.lastname: Slavcev
mesh_terms: D000368:Aged; D000917:Antibody Formation / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000097:blood* / Q000401:mortality*; D006680:HLA Antigens / Q000276:immunology; D017228:Hepatocyte Growth Factor / Q000097:blood* / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D017730:Ki-1 Antigen / Q000097:blood* / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D015996:Survival Rate; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D015415:Biomarkers; C066855:HGF protein, human; D006680:HLA Antigens; D007518:Isoantibodies; D017730:Ki-1 Antigen; D017228:Hepatocyte Growth Factor