title: BSA adsorption on differently charged polystyrene nanoparticles using isothermal titration calorimetry and the influence on cellular uptake.
abstract: BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the particle, was studied using ITC. Different thermodynamic data such as enthalpy changes upon binding and stoichiometry of the systems were determined and discussed. The degree of surface coverage with BSA was calculated using the thermodynamic data. The cellular uptake of particles before and after BSA adsorption was studied using HeLa cells in the presence and absence of supplemented FCS in the cell culture medium.
authors.affiliation: Max Planck Institute for Polymer Research, Mainz, Germany.
authors.forename: Grit
authors.initials: G
authors.lastname: Baier
authors.forename: Cristiane
authors.initials: C
authors.lastname: Costa
authors.forename: Anke
authors.initials: A
authors.lastname: Zeller
authors.forename: Daniela
authors.initials: D
authors.lastname: Baumann
authors.forename: Claudia
authors.initials: C
authors.lastname: Sayer
authors.forename: Pedro H H
authors.initials: PH
authors.lastname: Araujo
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
authors.forename: Anna
authors.initials: A
authors.lastname: Musyanovych
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
mesh_terms: D000327:Adsorption; D000465:Algorithms; D002151:Calorimetry; D003470:Culture Media; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles; D010316:Particle Size; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D055672:Static Electricity; D013499:Surface Properties; D017186:Titrimetry
chemical_list: D003470:Culture Media; D011137:Polystyrenes; D012710:Serum Albumin, Bovine