title: Identification and characterization of protective epitope of Trichinella spiralis paramyosin.
abstract: Trichinella spiralis paramyosin (Ts-Pmy) is a protective antigen that induces partial immunity against T. spiralis infection in mice. To identify protective epitope of Ts-Pmy, a monoclonal antibody (mAb) 7E2 against the recombinant protein was generated, which partially protected against T. spiralis infection following passive transfer. The mAb was used to screen a random phage-displayed peptide library. Ten positive clones were identified, most of which matched amino acids 88-107 or 108-127 of Ts-Pmy. Expression of overlapping fragments of Ts-Pmy in E. coli confirmed that region 88-107 was specifically recognized by 7E2. A peptide based on this epitope region (YX1) was synthesized and shown to compete with native Ts-Pmy for binding to 7E2. Mice immunized with KLH-conjugated YX1 were protected against T. spiralis larval challenge. The identification of a protective epitope within Ts-Pmy highlights the possibility of developing a subunit vaccine against T. spiralis infection.
authors.affiliation: Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Wei
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wang
authors.forename: Shijuan
authors.initials: S
authors.lastname: Cui
authors.forename: Xinping
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D018604:Epitope Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D016756:Immunoglobulins, Intravenous / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000517:prevention & control; D014335:Tropomyosin / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D016756:Immunoglobulins, Intravenous; D011994:Recombinant Proteins; D014335:Tropomyosin; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic