title: Protective effects of Chlorella-derived peptide on UVB-induced production of MMP-1 and degradation of procollagen genes in human skin fibroblasts.
abstract: UV exposure is known to induce premature aging, which is mediated by matrix metalloproteinase-1 (MMP-1) activity. MMP-1 mRNA expression is up-regulated by elevated cysteine-rich 61 (CYR61) and monocyte chemoattractant protein-1 (MCP-1) via action of transcription factor AP-1. Collagen is degraded by MMP-1 activity but synthesized by transforming growth factor-β (TGF-β) signal. Chlorella has been shown to inhibit UVB-induced MMP-1 level, however its regulatory molecular mechanisms have not been studied. In this study, Chlorella derived peptide (CDP) was added to skin fibroblasts after UVB irradiation and the expression of MMP-1, CYR61, procollagen, c-fos, c-jun, and TGF-β receptor (TbRII) mRNA and MCP-1 production were investigated. CDP (10 or 5mg/ml) diminished UVB-induced MMP-1 and CYR61 mRNA expression and MCP-1 production, whereas, UVB-suppressed procollagen and TbRII mRNA was restored by CDP treatment. UVB-induced c-fos and c-jun expressions were also inhibited by the CDP treatment. Taken together, CDP inhibits UVB-induced MMP-1 expression in skin fibroblasts by suppressing expression of AP-1 and CYR61 and MCP-1 production.
authors.affiliation: Department of Pharmacy, Chia-Nan University of Pharmacy & Science, Tainan, Taiwan.
authors.forename: Chiu-Lan
authors.initials: CL
authors.lastname: Chen
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Liou
authors.forename: Su-Jong
authors.initials: SJ
authors.lastname: Chen
authors.forename: Mei-Fen
authors.initials: MF
authors.lastname: Shih
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D002708:Chlorella / Q000737:chemistry*; D055515:Cysteine-Rich Protein 61 / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D011347:Procollagen / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: C111835:CCL2 protein, human; C501848:CCN1 protein, human; D018932:Chemokine CCL2; D055515:Cysteine-Rich Protein 61; D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins; D011347:Procollagen; D018808:Transcription Factor AP-1; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1