title: Molecular Methods for Haemophilus influenzae.
abstract: The speciesHaemophilus influenzae belongs to the genus Haemophilus and the family Pasteurellaceae. H influenzae are small, nonmotile, nonspore forming, Gram-negative, pleomorphic rods that range in shape from coccobacilli to long filaments. They require X and V factors (hemin and NAD, respectively) for aerobic growth, and may be facultatively anaerobic (1) Encapsulated H. influenzae are classified into six antigenically distinct serotypes (a-f), and have a clonal population structure with two major global subdivisions (I and II) (2,3). Nonencapsulated H. infuenzae (NCHi) appear to have a nonclonal population composition, but a broader analysis of NCHi in the future may reveal that the population is not so distinct from encapsulated H influenzae (4). Both encapsulated H influenzae and NCHi exhibit wide genetic diversity (5).
authors.affiliation: Paediatric Infectious Diseases Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Herbert
authors.forename: D
authors.initials: D
authors.lastname: Crook
authors.forename: E R
authors.initials: ER
authors.lastname: Moxon