title: The degradation of phytate by microbial and wheat phytases is dependent on the phytate matrix and the phytase origin.
abstract: BACKGROUND
  Phytases increase utilization of phytate phosphorus in feed. Since wheat is rich in endogenous phytase activity it was examined whether wheat phytases could improve phytate degradation compared to microbial phytases. Moreover, it was investigated whether enzymatic degradation of phytate is influenced by the matrix surrounding it. Phytate degradation was defined as the decrease in the sum of InsP₆ + InsP₅.
  
  
  RESULTS
  Endogenous wheat phytase effectively degraded wheat Ins₆ + InsP₅ at pH 4 and pH 5, while this was not true for a recombinant wheat phytase or phytase extracted from wheat bran. Only microbial phytases were able to degrade InsP₆ + InsP₅ in the entire pH range from 3 to 5, which is relevant for feed applications. A microbial phytase was efficient towards InsP₆ + InsP₅ in different phytate samples, whereas the ability to degrade InsP₆ + InsP₅ in the different phytate samples ranged from 12% to 70% for the recombinant wheat phytase.
  
  
  CONCLUSIONS
  Wheat phytase appeared to have an interesting potential. However, the wheat phytases studied could not improve phytate degradation compared to microbial phytases. The ability to degrade phytate in different phytate samples varied greatly for some phytases, indicating that phytase efficacy may be affected by the phytate matrix.
authors.affiliation: Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark.
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Brejnholt
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Dionisio
authors.forename: Vibe
authors.initials: V
authors.lastname: Glitsoe
authors.forename: Lars K
authors.initials: LK
authors.lastname: Skov
authors.forename: Henrik
authors.initials: H
authors.lastname: Brinch-Pedersen
mesh_terms: D010832:6-Phytase / Q000378:metabolism* / Q000494:pharmacology; D000821:Animal Feed; D001407:Bacillus / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D016226:Phosphorus, Dietary / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012639:Seeds; D014908:Triticum / Q000201:enzymology / Q000378:metabolism*
chemical_list: D016226:Phosphorus, Dietary; D010940:Plant Proteins; D011994:Recombinant Proteins; D010833:Phytic Acid; D010832:6-Phytase