title: Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor.
abstract: Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist exposure and of the functional recovery of activated protease activated receptor-1 (PAR(1)). The biosensor manifests that the persistency of PAR(1) signaling is dependent on the agonists, which, in turn, affects the receptor resensitization process. This study demonstrates a new means to differentiate ligand-directed functional selectivity on trafficking of receptors.
authors.affiliation: Biochemical Technologies, Science and Technology Division, Corning Inc., Corning, NY 14831, United States.
authors.forename: Vasiliy
authors.initials: V
authors.lastname: Goral
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Ye
authors.initials: Y
authors.lastname: Fang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D008024:Ligands; D046210:Microfluidic Analytical Techniques; D055620:Optical Phenomena; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044463:Receptor, PAR-1 / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013379:Substrate Specificity
chemical_list: D008024:Ligands; D012333:RNA, Messenger; D044463:Receptor, PAR-1