title: Automated analysis of FISH-stained HER2/neu samples with Metafer.
abstract: The HER2/neu gene (also known as ERBB2) is located on chromosome 17 (q11.2-q12) and encodes a glycoprotein known to be a member of the epidermal growth factor receptor family. Clinically, the determination of its amplification status is of utmost importance, as 10-35% of invasive human breast carcinomas come along with HER2/neu overexpression, and treatment has to be adjusted accordingly. Here a method to analyze HER2 FISH samples with digital microscopy, using the slide scanning -platform Metafer PV (MetaSystems, Altlussheim, Germany), is presented. Metafer PV is a system for the automation of HER2/neu FISH assay analysis of samples hybridized with the Abbott(™) PathVysion(®) probe kit.
authors.affiliation: MetaSystems, Altlussheim, Germany. cschunck@metasystems.de
authors.forename: Christian
authors.initials: C
authors.lastname: Schunck
authors.forename: Eiman
authors.initials: E
authors.lastname: Mohammad
mesh_terms: D001331:Automation; D005557:Formaldehyde; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D016612:Paraffin Embedding; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation
chemical_list: D005557:Formaldehyde; D007202:Indicators and Reagents; D018719:Erb-b2 Receptor Tyrosine Kinases