title: Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex.
abstract: To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress.
authors.affiliation: Département de Virologie Structurale, Architecture et Fonction des Macromolécules Biologiques, UMR 6098, 163 Avenue de Luminy, 13288 Marseille CEDEX 09, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Debarnot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Imbert
authors.forename: François
authors.initials: F
authors.lastname: Ferron
authors.forename: Laure
authors.initials: L
authors.lastname: Gluais
authors.forename: Isabelle
authors.initials: I
authors.lastname: Varlet
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papageorgiou
authors.forename: Mickaël
authors.initials: M
authors.lastname: Bouvet
authors.forename: Julien
authors.initials: J
authors.lastname: Lescar
authors.forename: Etienne
authors.initials: E
authors.lastname: Decroly
authors.forename: Bruno
authors.initials: B
authors.lastname: Canard
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008780:Methyltransferases / Q000737:chemistry*; D008969:Molecular Sequence Data; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: C558878:Nsp10 protein, SARS virus; D017361:Viral Nonstructural Proteins; D008780:Methyltransferases; C558879:Nsp16 protein, SARS virus; D012324:RNA-Dependent RNA Polymerase