title: A rapid and easy method for the qualitative detection of intracellular deposition of inhaled nanoparticles.
abstract: In recent years, nanoparticle exposure risk has drawn increasing attention from the research community and the general public. However, analysis of nanoparticles is hindered by their small size, which prevents the development of methods for their detection in cells and tissues. For risk assessment of nanoparticle exposure, it is important to measure the exact amount of deposited material in pulmonary tissue. Using a nanoparticle exposure device, A/JJmsSlc mice were chronically exposed transtracheally to anatase-type titanium dioxide particles. A microscope-integrated laser Raman spectrometer was used to detect differentially stained macrophages in a pulmonary wash obtained from the mice exposed to the particles. This detection method allowed rapid and easy sample collection and qualitative analysis, and the method may be useful for conducting large-scale evaluations in workers exposed to environments heavily contaminated with nanoparticles.
  
  
  BACKGROUND
  This paper discusses a microscope-integrated laser Raman spectrometer method to measure the exact amount of nanoparticles deposited in pulmonary tissue. This method allows rapid sample collection, qualitative analysis, and may be useful for large-scale evaluations.
authors.affiliation: Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan. nakajima@radbio.med.osaka-u.ac.jp
authors.forename: Hiroo
authors.initials: H
authors.lastname: Nakajima
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Ozaki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hongyo
authors.forename: Isao
authors.initials: I
authors.lastname: Narama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Todo
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D019570:Inhalation Exposure / Q000032:analysis*; D008168:Lung / Q000166:cytology*; D008264:Macrophages / Q000166:cytology; D008297:Male; D051379:Mice; D008853:Microscopy / Q000379:methods; D053758:Nanoparticles / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors; D014025:Titanium / Q000032:analysis*
chemical_list: C009495:titanium dioxide; D014025:Titanium