title: Construction of tissue-engineered composite intervertebral disc and preliminary morphological and biochemical evaluation.
abstract: The aim of this study was to construct tissue-engineered composite intervertebral disc (IVD) consisting of demineralized bone matrix gelatin (DBMG) and collagen II/hyaluronate/chondroitin-6-sulfate (CII/HyA-CS) scaffolds seeded with anulus fibrosus (AF) and nucleus pulposus (NP) cells, respectively. The cell-scaffold hybrids were implanted in the subcutaneous space of the dorsum of athymic mice and harvested at 4, 8, and 12 weeks. At each time point, the gross and histological morphology and biochemical properties were evaluated. Our results are as following: the gross morphology and histology of the composite resembled those of native IVD. Morphological studies revealed progressive tissue formation and junction integration between AF and NP regions. Biochemical composition detection indicated that the content of DNA, proteoglycan and hydroxyproline increased with time, and were similar to native tissue at 12 weeks. All these results demonstrated the feasibility of creating a tissue-engineered composite IVD with similar morphological and biochemical properties to the native tissue.
authors.affiliation: Department of Orthopedics, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhuang
authors.forename: B
authors.initials: B
authors.lastname: Huang
authors.forename: C Q
authors.initials: CQ
authors.lastname: Li
authors.forename: L T
authors.initials: LT
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Pan
authors.forename: W J
authors.initials: WJ
authors.lastname: Zheng
authors.forename: G
authors.initials: G
authors.lastname: Luo
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002809:Chondroitin Sulfates / Q000737:chemistry; D024043:Collagen Type II / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry; D007403:Intervertebral Disc / Q000737:chemistry* / Q000166:cytology* / Q000637:transplantation; D051379:Mice; D008819:Mice, Nude; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024043:Collagen Type II; D005780:Gelatin; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D004247:DNA