title: In vitro assessment of macrophage attachment and phenotype on polymerized phospholipid bilayers.
abstract: Phosphatidyl choline (PC)-based materials have been found to be resistant to nonspecific protein adhesion in vitro. In this study, a PC-based planar supported phospholipid bilayer composed of 1,2-bis[10-(2',4'-hexadienoyloxy)decanoyl]-sn-glycero-3-phosphocholine (bis-SorbPC or BSPC) was generated on piranha-treated silicon wafers by vesicle deposition. The bilayer was polymerized with redox initiation forming a stable 4-nm thick coating. Polymerized lipid bilayers (PLBs) were characterized and tested for uniformity, with ellipsometry and contact angle. Cellular adhesion and morphological changes in RAW 264.7 macrophages were investigated in vitro on PLBs and compared to bare silicon controls. Fluorescent and scanning electron microscopy were used to observe changes in cellular morphology. The PLBs showed much lower cellular adhesion than bare silicon controls. Of the cells that attached to the PLBs, a very low percentage showed the same morphological expressions seen on the controls. It is hypothesized that proteins adsorb to the defects in the PLBs, caused by incomplete polymerization, and this mediates the observed minimal cellular attachment and morphological changes. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: , 2011.
authors.affiliation: Department of Materials Science and Engineering, The University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Page
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Heitz
authors.forename: James R
authors.initials: JR
authors.lastname: Joubert
authors.forename: John P
authors.initials: JP
authors.lastname: Keogh
authors.forename: Tim
authors.initials: T
authors.lastname: Sparer
authors.forename: S Scott
authors.initials: SS
authors.lastname: Saavedra
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002460:Cell Line; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D010641:Phenotype; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010743:Phospholipids; D011108:Polymers; C077390:1,2-bis(10(2',4'-hexadienoyloxy)decanoyl)-sn-glycero-3-phosphocholine