title: One-pot fluorescence detection of multiple analytes in homogenous solution based on noncovalent assembly of single-walled carbon nanotubes and aptamers.
abstract: We have developed a new multicolor fluorescent sensing system to detect multiple analytes in one pot. This design is based on the noncovalent assembly of dye-labeled aptamer with single-walled carbon nanotubes (SWNTs) by π-stacking between the nucleotide bases and the SWNTs sidewalls. In the presence of the targets, the aptamer-target binding separates the assembly of dye-labeled aptamers and SWNTs, resulting in the restoration of fluorescence signal of the dye labeled with aptamers. As a proof of concept, we demonstrate that a two-color fluorescent system can simultaneously and selectively detect two targets (thrombin and adenosine triphosphate) in a single solution. Since the method is mix-and-detect manner, the present strategy is simple and cost-effective.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
authors.forename: Yuanfu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Baoxin
authors.initials: B
authors.lastname: Li
authors.forename: Chungeng
authors.initials: C
authors.lastname: Yan
authors.forename: Lihui
authors.initials: L
authors.lastname: Fu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005453:Fluorescence; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D037742:Nanotubes, Carbon; D012996:Solutions; D000255:Adenosine Triphosphate; D013917:Thrombin