title: Rapid and efficient detection of Mycobacterium tuberculosis in respiratory and non-respiratory samples.
abstract: The detection of Mycobacterium tuberculosis using a new commercial real-time polymerase chain reaction (PCR) assay (Xpert™ MTB/RIF) was evaluated on 91 respiratory and 89 non-respiratory samples recovered from 132 patients suspected of tuberculosis (TB). Overall, 31 (17.2%) of the 180 samples, including 17 respiratory and 14 non-respiratory (respectively 17 and 12 PCR-positive), yielded M. tuberculosis on culture. The sensitivity and specificity of PCR were respectively 100% and 100%, and 85.7% and 97.3% for respiratory and non-respiratory samples. Although the test is validated only for respiratory samples, our findings suggest that it could be useful for the diagnosis of extra-pulmonary TB.
authors.affiliation: Service de Microbiologie, Centre Hospitalière Universitaire Côte de Nacre, Caen, France.
authors.forename: B
authors.initials: B
authors.lastname: Malbruny
authors.forename: G
authors.initials: G
authors.lastname: Le Marrec
authors.forename: K
authors.initials: K
authors.lastname: Courageux
authors.forename: R
authors.initials: R
authors.lastname: Leclercq
authors.forename: V
authors.initials: V
authors.lastname: Cattoir
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology