title: Pseudomonas sax genes overcome aliphatic isothiocyanate-mediated non-host resistance in Arabidopsis.
abstract: Most plant-microbe interactions do not result in disease; natural products restrict non-host pathogens. We found that sulforaphane (4-methylsulfinylbutyl isothiocyanate), a natural product derived from aliphatic glucosinolates, inhibits growth in Arabidopsis of non-host Pseudomonas bacteria in planta. Multiple sax genes (saxCAB/F/D/G) were identified in Pseudomonas species virulent on Arabidopsis. These sax genes are required to overwhelm isothiocyanate-based defenses and facilitate a disease outcome, especially in the young leaves critical for plant survival. Introduction of saxCAB genes into non-host strains enabled them to overcome these Arabidopsis defenses. Our study shows that aliphatic isothiocyanates, previously shown to limit damage by herbivores, are also crucial, robust, and developmentally regulated defenses that underpin non-host resistance in the Arabidopsis-Pseudomonas pathosystem.
authors.affiliation: John Innes Centre, Norwich NR4 7UH, UK. jun.fan@bbsrc.ac.uk
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
authors.forename: Casey
authors.initials: C
authors.lastname: Crooks
authors.forename: Gary
authors.initials: G
authors.lastname: Creissen
authors.forename: Lionel
authors.initials: L
authors.lastname: Hill
authors.forename: Shirley
authors.initials: S
authors.lastname: Fairhurst
authors.forename: Peter
authors.initials: P
authors.lastname: Doerner
authors.forename: Chris
authors.initials: C
authors.lastname: Lamb
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D005798:Genes, Bacterial; D005961:Glucosinolates / Q000378:metabolism; D054884:Host-Pathogen Interactions; D017879:Isothiocyanates / Q000378:metabolism / Q000494:pharmacology; D009876:Operon; D010935:Plant Diseases / Q000382:microbiology; D010936:Plant Extracts / Q000494:pharmacology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D013454:Sulfoxides; D013861:Thiocyanates / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D005961:Glucosinolates; D017879:Isothiocyanates; D010936:Plant Extracts; D013454:Sulfoxides; D013861:Thiocyanates; C016766:sulforaphane