title: Native Australian fruit polyphenols inhibit cell viability and induce apoptosis in human cancer cell lines.
abstract: Apoptosis is one of the most critical forms of defense against cancer, and the induction of apoptosis by dietary polyphenols represents significant potential for cancer preventive activity. The present study examined polyphenols extracted from selected native Australian fruits--Illawarra plum (Podocarpus elatus Endl., Podocarpaceae), Kakadu plum (Terminalia ferdinandiana Exell, Combretaceae), muntries (Kunzea pomifera F. Muell., Myrtaceae), and native currant (Acrotriche depressa R.Br., Epacridaceae)--for antiproliferative activity against a panel of cancer and normal cell lines. Each fruit selectively inhibited the growth of cancer cell lines in a dose-dependent manner. The mechanism of growth inhibition of the human promyelocytic leukaemia cells (HL-60) was determined to be apoptosis by morphological assessment, DNA fragmentation, flow cytometry, and caspase-3 induction. Furthermore, Kakadu plum was found to activate caspase-7, -9, and poly (ADP-ribose) polymerase (PARP), suggesting it acts via the intrinsic apoptosis pathway. The same fruit also caused direct DNA damage in colon adenocarcinoma cells (HT-29) as detected using the cytokinesis-block micronucleus cytome (CBMN Cyt) assay.
authors.affiliation: Food Futures Flagship, CSIRO Food and Nutritional Sciences, North Ryde, New South Wales, Australia. Aaron.Tan@csiro.au
authors.forename: Aaron C
authors.initials: AC
authors.lastname: Tan
authors.forename: Izabela
authors.initials: I
authors.lastname: Konczak
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ramzan
authors.forename: Daniel M-Y
authors.initials: DM
authors.lastname: Sze
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001315:Australia; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D018890:Chemoprevention; D029764:Combretaceae / Q000737:chemistry; D053938:DNA Fragmentation; D029791:Ericaceae / Q000737:chemistry; D005419:Flavonoids / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D027822:Myrtaceae / Q000737:chemistry; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D059808:Polyphenols; D064028:Tracheophyta / Q000737:chemistry
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7