title: Cloning and phylogenetic analysis of Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) from Indian dromedaries (Camelus dromedarius).
abstract: The cDNAs of two proinflammatory cytokines viz., IL-6 and TNF-α from dromedarian camels were amplified by PCR using bactrian camel sequences and subsequently cloned for sequence analysis. Relationship based on amino acid revealed that dromedarian camel IL-6 shared 99.5% identity both at nucleotide and amino acid level with bactrian camel IL-6 and in case of TNF-α, the identity of dromedarian camel was 99.4% and 99.1% at nucleotide and amino acid level, respectively with that of bactrian camel. Phylogenetic analysis based on their amino acid sequences indicated the close relationship in these cytokine genes between dromedarian camel and other members of camelids.
authors.affiliation: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India. camelnag@yahoo.com
authors.forename: G
authors.initials: G
authors.lastname: Nagarajan
authors.forename: Shelesh Kumar
authors.initials: SK
authors.lastname: Swami
authors.forename: S K
authors.initials: SK
authors.lastname: Ghorui
authors.forename: K M L
authors.initials: KM
authors.lastname: Pathak
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.forename: N V
authors.initials: NV
authors.lastname: Patil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002162:Camelus / Q000145:classification* / Q000235:genetics*; D003001:Cloning, Molecular; D015850:Interleukin-6 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000235:genetics*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha