title: Determination of metformin hydrochloride using precolumn derivatization with acetaldehyde and capillary electrophoresis coupled with electrochemiluminescence.
abstract: A novel method was developed using capillary electrophoresis (CE) coupled with tris(2,2'-bipyridyl)ruthenium(II) electrogenerated chemiluminescence (ECL) for highly sensitive detection of metformin hydrochloride (MH) derivatizatized with acetaldehyde. The precolumn derivatization of MH with acetaldehyde was performed in phosphate buffer solution (0.3 mol/L, pH 7.5) at room temperature for 120 min. The effects of acetaldehyde concentration, buffer pH, electrokinetic voltage and injection time were investigated. Under optimized detection conditions, the MH ECL detection sensitivity was more than 120 times that without derivatization. The linear concentration range for MH was 0.001-15.00 μg/mL (with a correlation coefficient of 0.9992). The detection limit was 0.31 ng/mL with a signal:noise ratio of 3. The recoveries of MH in human urine were in the range 98.50-99.72%. Copyright © 2011 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, People's Republic of China. dengby16@163.com
authors.forename: Biyang
authors.initials: B
authors.lastname: Deng
authors.forename: Aihong
authors.initials: A
authors.lastname: Shi
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Kang
authors.forename: Linqiu
authors.initials: L
authors.lastname: Li
mesh_terms: D000079:Acetaldehyde / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000032:analysis*; D057230:Limit of Detection; D049449:Luminescence; D008297:Male; D008687:Metformin / Q000032:analysis*; D008875:Middle Aged; D015203:Reproducibility of Results
chemical_list: D007004:Hypoglycemic Agents; D008687:Metformin; D000079:Acetaldehyde