title: Coherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object.
abstract: A method to reduce coherent noise in digital holographic phase contrast microscopy is proposed. By slightly shifting the specimen, a series of digital holograms with different coherent noise patterns is recorded. Each hologram is reconstructed individually, while the different phase tilts of the reconstructed complex amplitudes due to the specimen shifts are corrected in the hologram plane by using numerical parametric lens method. Afterward, the lateral displacements of the phase maps from different holograms are compensated in the image plane by using digital image registration method. Thus, all phase images have same distribution, but uncorrelated coherent noise patterns. By a proper averaging procedure, the coherent noise of phase contrast image is reduced significantly. The experimental results are given to confirm the proposed method.
authors.affiliation: School of Instrumentation Science & Optoelectronics Engineering, Beihang University, Beijing, China. panfeng@buaa.edu.cn
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.forename: Wen
authors.initials: W
authors.lastname: Xiao
authors.forename: Shuo
authors.initials: S
authors.lastname: Liu
authors.forename: FanJing
authors.initials: F
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Rong
authors.forename: Rui
authors.initials: R
authors.lastname: Li
mesh_terms: D000465:Algorithms; D016477:Artifacts; D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D013048:Specimen Handling / Q000379:methods*; D013382:Subtraction Technique