title: [Simultaneous analysis of 4 non-steroidal anti-inflammatory drug residues in mutton muscle using high performance liquid chromatography assisted by ultrasonic-microwave extraction].
abstract: A method for the simultaneous determination of 4 non-steroidal anti-inflammatory drug (NSAID) residues, including flunixin meglumine, meloxicam, diclofenac sodium and ketoprofen, in mutton muscle was developed using high performance liquid chromatography assisted by ultrasonic-microwave extraction. The NSAIDs were extracted with acidified ethanol and purified by a diatomite column. The subsequent analysis of NSAIDs was achieved on a Hypersil C18 column (250 mm x 4.6 mm, 5 microm) with the mobile phase of acetonitrile-0.2% triethylamine (40 : 60, v/v, pH 3.5 adjusted by phosphoric acid) at a flow rate of 0.8 mL/min at 30 degrees C. The detection wavelength was set at 255 nm. The 4 NSAIDs were well separated within 20 min. The correlation coefficients for 4 NSAIDs were from 0.999 3 to 0.999 8 with the limits of detection (LOD, S/N = 3) of 5-10 microg/kg and the limits of quantification (LOQ, S/N = 10) of 15-30 microg/kg. The recoveries were in the range of 65.3% - 99.6% with the relative standard deviations (RSDs) less than 15%. This method is simple, rapid and highly sensitive, and can meet the requirement for the qualitative and quantitative analysis.
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. yfkang@shou.edu.cn
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Kang
authors.forename: Shiwen
authors.initials: S
authors.lastname: Zou
authors.forename: Wuping
authors.initials: W
authors.lastname: Duan
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003002:Clonixin / Q000031:analogs & derivatives / Q000032:analysis; D004008:Diclofenac / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D000077239:Meloxicam; D008872:Microwaves; D012756:Sheep; D052616:Solid Phase Extraction / Q000379:methods*; D013843:Thiazines / Q000032:analysis; D013844:Thiazoles / Q000032:analysis; D014465:Ultrasonics / Q000379:methods
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013843:Thiazines; D013844:Thiazoles; D004008:Diclofenac; C014558:flunixin meglumine; D003002:Clonixin; D000077239:Meloxicam