title: Quantitative tracking of T cell clones after haematopoietic stem cell transplantation.
abstract: Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.
authors.affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.
authors.forename: Ilgar Z
authors.initials: IZ
authors.lastname: Mamedov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Britanova
authors.forename: Dmitriy A
authors.initials: DA
authors.lastname: Bolotin
authors.forename: Anna V
authors.initials: AV
authors.lastname: Chkalina
authors.forename: Dmitriy B
authors.initials: DB
authors.lastname: Staroverov
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Zvyagin
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Kotlobay
authors.forename: Maria A
authors.initials: MA
authors.lastname: Turchaninova
authors.forename: Denis A
authors.initials: DA
authors.lastname: Fedorenko
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Novik
authors.forename: George V
authors.initials: GV
authors.lastname: Sharonov
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.forename: Dmitriy M
authors.initials: DM
authors.lastname: Chudakov
authors.forename: Yuri B
authors.initials: YB
authors.lastname: Lebedev
mesh_terms: D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology / Q000628:therapy*; D002470:Cell Survival; D002999:Clone Cells; D005500:Follow-Up Studies; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014182:Transplantation, Autologous
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta