title: Structural and functional analysis of the N-terminal region of death receptor 5.
abstract: OBJECTIVE
  To investigate the structure and function of the N-terminal region (NTR) of death receptor 5 (DR5).
  
  
  METHODS
  A series of deletions of the DR5 extracellular domain (DR5-ECD) proteins were expressed in E.coli. and purified by affinity chromatography. The binding ability of these deletant proteins to AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by immunoblotting and ELISA.
  
  
  RESULTS
  Recombinant DR5-ECD proteins containing the NTR were recognized and bound by AD5-10, while the other deletant proteins without the NTR failed to interact with AD5-10.
  
  
  CONCLUSIONS
  There is an AD5-10 targeting site in the NTR of DR5, which may play a role in developing novel immunotherapies for cancers.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,CAMS and PUMC, Beijing 100005, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Feng
authors.forename: Chen
authors.initials: C
authors.lastname: Cai-feng
authors.forename: Liu
authors.initials: L
authors.lastname: Shi-lian
authors.forename: Zheng
authors.initials: Z
authors.lastname: De-xian
authors.forename: Liu
authors.initials: L
authors.lastname: Yan-xin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001665:Binding Sites; D017353:Gene Deletion; D005818:Genetic Engineering; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D011485:Protein Binding; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; C505640:Tnfrsf10b protein, mouse