title: Hardenbergia virus A, a novel member of the family Betaflexiviridae from a wild legume in Southwest Australia.
abstract: A 6,936-nucleotide sequence representing the complete genome of a plant virus, tentatively named Hardenbergia virus A, was obtained from Hardenbergia comptoniana. The predicted structure of the genome is a 5' untranslated region (5'UTR), a 258-kDa polyprotein in open reading frame 1 (ORF1), a 43-kDa protein in ORF2, 3'UTR and poly(A) tail. The N-terminal region of ORF1 contains a replicase protein, and the C-terminal region contains a coat protein. The protein encoded by ORF2 has homology with p30-like viral movement proteins. The predicted genome organization and phylogeny of gene products places Hardenbergia virus A within the family Betaflexiviridae.
authors.affiliation: Plant Virus Section, Plant Biotechnology Research Group, Western Australian State Agricultural Biotechnology Centre, Murdoch University, Perth, WA, 6150, Australia. s.wylie@murdoch.edu.au
authors.forename: Stephen
authors.initials: S
authors.lastname: Wylie
authors.forename: Michael
authors.initials: M
authors.lastname: Jones
mesh_terms: D001315:Australia; D007887:Fabaceae / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*