title: Corn fiber, cobs and stover: enzyme-aided saccharification and co-fermentation after dilute acid pretreatment.
abstract: Three corn feedstocks (fibers, cobs and stover) available for sustainable second generation bioethanol production were subjected to pretreatments with the aim of preventing formation of yeast-inhibiting sugar-degradation products. After pretreatment, monosaccharides, soluble oligosaccharides and residual sugars were quantified. The size of the soluble xylans was estimated by size exclusion chromatography. The pretreatments resulted in relatively low monosaccharide release, but conditions were reached to obtain most of the xylan-structures in the soluble part. A state of the art commercial enzyme preparation, Cellic CTec2, was tested in hydrolyzing these dilute acid-pretreated feedstocks. The xylose and glucose liberated were fermented by a recombinant Saccharomyces cerevisiae strain. In the simultaneous enzymatic saccharification and fermentation system employed, a concentration of more than 5% (v/v) (0.2g per g of dry matter) of ethanol was reached.
authors.affiliation: Royal Nedalco, Lelyweg 29, 4612 PS, Bergen op Zoom, The Netherlands. d.eylen@nedalco.nl
authors.forename: David
authors.initials: D
authors.lastname: Van Eylen
authors.forename: Femke
authors.initials: F
authors.lastname: van Dongen
authors.forename: Mirjam
authors.initials: M
authors.lastname: Kabel
authors.forename: Jan
authors.initials: J
authors.lastname: de Bont
mesh_terms: D000143:Acids / Q000737:chemistry*; D050260:Carbohydrate Metabolism; D004798:Enzymes / Q000378:metabolism*; D005285:Fermentation; D003313:Zea mays
chemical_list: D000143:Acids; D004798:Enzymes