title: Evaluation of the antioxidant and hepatoprotective effect of Majoon-e-Dabeed-ul-ward against carbon tetrachloride induced liver injury.
abstract: The present study was designed to demonstrate the antioxidant and hepatoprotective effect of Majoon-e-Dabeed-ul-ward, a Unani herbal formulation. The Majoon-e-Dabeed-ul-ward (MD) at the doses of 250, 500 and 1000 mg/kg, p.o. was administered after carbon-tetrachloride (CCl(4); 1.5 ml/kg, i.p. once only) intoxication. Treatment with MD at three doses brought the levels of aspartate transaminase, alanine transaminase, albumin and urea in dose dependent manner. Signification reduction was found in TBARS content and restored the level of reduced glutathione, adenosine triphosphatase, and glucose-6-phosphatase in liver. Therapy of MD showed its protective effect on biochemical and histopathological observation at all the three doses in a dose dependent manner. The study conducted showed that MD possesses strong hepatoprotective activity as decrease the hexobarbitone sleep time and improvement in physiological parameter, excretory capacity (BSP retention time) was seen. DPPH and H(2)O(2) scavenging effects indicated its potent antioxidant activities. The results revealed that MD could afford significant dose-dependent protection against CCl(4) induced hepatocellular injury.
authors.affiliation: School of Studies in Zoology, Jiwaji University, Gwalior 474011, MP, India. shakya_arvind@yahoo.com
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Shakya
authors.forename: Neetu
authors.initials: N
authors.lastname: Sharma
authors.forename: Monika
authors.initials: M
authors.lastname: Saxena
authors.forename: Sadhana
authors.initials: S
authors.lastname: Shrivastava
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Shukla
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000737:chemistry; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000627:therapeutic use; D006861:Hydrogen Peroxide / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008111:Liver Function Tests; D019367:Medicine, Unani; D051379:Mice; D010851:Picrates / Q000737:chemistry; D028321:Plant Preparations / Q000008:administration & dosage / Q000627:therapeutic use*; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D028321:Plant Preparations; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride; C004931:1,1-diphenyl-2-picrylhydrazyl