title: Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform.
abstract: A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GO(x) ) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GO(x) on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use.
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Jia
authors.forename: Miao
authors.initials: M
authors.lastname: Cui
authors.forename: Chuan
authors.initials: C
authors.lastname: Dong
authors.forename: Shaomin
authors.initials: S
authors.lastname: Shuang
authors.forename: Yuen
authors.initials: Y
authors.lastname: Kwan
authors.forename: Martin M F
authors.initials: MM
authors.lastname: Choi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D013696:Temperature
chemical_list: D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose