title: The synergistic effects of cytomegalovirus IE2 and myocardin on cardiomyocyte hypertrophy.
abstract: Human cytomegalovirus immediate early proteins (CMV IEs) are involved in transcriptional activities of both host and virus gene expression. This study shows that the transcriptional activity of myocardin in regulating cardiomyocyte hypertrophy is enhanced by co-expressing CMV IE2. Forced expression of IE2 increases the augmented cell size of neonatal rat cardiac myocytes induced by myocardin, as well as the mRNA and protein levels of hypertrophic genes, whereas deletion of CArG boxes in the atrial natriuretic factor (ANF) promoter attenuates the effect of CMV IE2 with myocardin. In conclusion, CMV IE2 synergistically stimulates myocardin transactivity in the hypertrophic marker gene ANF in a CArG box-dependent manner. Our study indicates that the association of CMV IEs with myocardin-induced transcription may be involved in myocardin-mediated cardiac hypertrophy.
authors.affiliation: Institute of Cell and Molecular Biology, Medical School, Wuhan University of Science and Technology, Wuhan, Hubei Province 430065, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Liao
authors.forename: Chenyu
authors.initials: C
authors.lastname: Wu
authors.forename: Junyan
authors.initials: J
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.forename: Cailian
authors.initials: C
authors.lastname: Cheng
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Dongsun
authors.initials: D
authors.lastname: Cao
authors.forename: Tong-Cun
authors.initials: TC
authors.lastname: Zhang
mesh_terms: D020856:AT Rich Sequence / Q000235:genetics; D000185:Actinin / Q000235:genetics / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D006984:Hypertrophy / Q000235:genetics / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D026362:Serum Response Factor / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D000099251:Myocardin
chemical_list: D000185:Actinin; D009320:Atrial Natriuretic Factor; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D026362:Serum Response Factor; D015534:Trans-Activators; D000099251:Myocardin; C069186:IE2 protein, Cytomegalovirus