title: Src family kinase potentiates the activity of nicotinic acetylcholine receptor in rat autonomic ganglion innervating urinary bladder.
abstract: Src family kinases (SFKs), one of the tyrosine kinase groups, are primary regulators of signal transductions that control cellular functions such as cell proliferation, differentiation, survival, metabolism, and other important roles of the cell. One of the crucial functions of SFKs is to regulate the activities of various neuronal channels. In this study, we investigated the modulatory action of SFK on nicotinic acetylcholine receptors (nAChRs) expressed in rat major pelvic ganglion (MPG) neurons innervating the urinary bladder. PP1 and PP2 (5 μM), selective Src-kinase inhibitors, attenuated ACh-induced ionic currents and [Ca²+](i) transients in MPG neurons, whereas PP3, an inactive analogue, had no effect. Blocking the tyrosine kinase activity of Src kinase by pp60 c-src inhibitory peptide also reduced the ACh-induced currents. Conversely, sodium orthovanadate (200 μM), a tyrosine phosphatase inhibitor, significantly augmented the ACh-induced currents. In the kinase assay, the activities of SFKs in MPG neurons were also inhibited by PP2, but not by PP3. These data suggests that SFKs may have a facilitative role on the synaptic transmission in rat pelvic autonomic ganglion.
authors.affiliation: Department of Basic Nursing Science, Keimyung University College of Nursing, Daegu, Republic of Korea.
authors.forename: Na-Hyun
authors.initials: NH
authors.lastname: Kim
authors.forename: Kyu-Sang
authors.initials: KS
authors.lastname: Park
authors.forename: Seung-Kuy
authors.initials: SK
authors.lastname: Cha
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Yoon
authors.forename: Byung-Il
authors.initials: BI
authors.lastname: Yeh
authors.forename: Kyou-Hoon
authors.initials: KH
authors.lastname: Han
authors.forename: In Deok
authors.initials: ID
authors.lastname: Kong
mesh_terms: D000818:Animals; D005725:Ganglia, Autonomic / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D001743:Urinary Bladder / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D047428:Protein Kinase Inhibitors; D011978:Receptors, Nicotinic; D019061:src-Family Kinases