title: Activating transcription factor 3 (ATF3) promotes sublytic C5b-9-induced glomerular mesangial cells apoptosis through up-regulation of Gadd45α and KLF6 gene expression.
abstract: The sublytic C5b-9 complexes can result in glomerular mesangial cells (GMCs) apoptosis, which involved in the initiation and development of rat Thy-1 nephritis. Activating transcription factor 3 (ATF3) is an immediate early gene for cells to cope with a variety of stress signals, and our previous study revealed that ATF3 could promote GMCs apoptosis attacked by sublytic C5b-9. But the mechanism of ATF3 promoting GMCs apoptosis triggered by sublytic C5b-9 attack has not been elucidated. In this study, the data showed that the expression of ATF3, growth arrest and DNA damage-45 alpha (Gadd45α), Krüppel-like factor 6 (KLF6) and proliferating cell nuclear antigen (PCNA) in the GMCs in response to sublytic C5b-9 stimulation for the indicated time was significantly increased, and ATF3 expression could lead to GMCs apoptosis through up-regulation of Gadd45α and KLF6, but not up-regulation of PCNA. Furthermore, Gadd45α was identified as a downstream target gene regulated by ATF3 directly, and KLF6 might be regulated by ATF3 in an indirect manner.
authors.affiliation: Department of Microbiology and Immunology, Nanjing Medical University, 140 Nanjing Hanzhong Road, Nanjing, PR China.
authors.forename: Kuanfeng
authors.initials: K
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wen
authors.initials: W
authors.lastname: Qiu
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Xia
authors.forename: Lisha
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D051700:Activating Transcription Factor 3 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000276:immunology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D015938:Complement Membrane Attack Complex / Q000009:adverse effects / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage; D015870:Gene Expression; D020868:Gene Silencing / Q000187:drug effects; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008156:Luciferases / Q000032:analysis; D050527:Mesangial Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D009393:Nephritis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010957:Plasmids; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D015533:Transcriptional Activation / Q000276:immunology; D014162:Transfection; D015854:Up-Regulation; D000097642:GADD45 Proteins
chemical_list: D051700:Activating Transcription Factor 3; D018797:Cell Cycle Proteins; D015938:Complement Membrane Attack Complex; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D008156:Luciferases; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; C499073:Gadd45a protein, rat; D000097642:GADD45 Proteins; C491357:Klf6 protein, rat