title: Impact of early life stress on alcohol consumption and on the short- and long-term responses to alcohol in adolescent female rats.
abstract: We examined the interaction between early life stress and vulnerability to alcohol in female rats exposed to prenatal restraint stress (PRS rats). First we studied the impact of PRS on ethanol preference during adolescence. PRS slightly increased ethanol preference per se, but abolished the effect of social isolation on ethanol preference. We then studied the impact of PRS on short- and long-term responses to ethanol focusing on behavioral and neurochemical parameters related to depression/anxiety. PRS or unstressed adolescent female rats received 10% ethanol in the drinking water for 4 weeks from PND30 to PND60. At PND60, the immobility time in the forced-swim test did not differ between PRS and unstressed rats receiving water alone. Ethanol consumption had no effect in unstressed rats, but significantly reduced the immobility time in PRS rats. In contrast, a marked increase in the immobility time was seen after 5 weeks of ethanol withdrawal only in unstressed rats. Hippocampal levels of neuropeptide Y (NPY) and mGlu1a metabotropic glutamate receptors were increased at the end of ethanol treatment only in unstressed rats. Ethanol treatment had no effect on levels of corticotropin-releasing hormone (CRH) in the hippocampus, striatum, and prefrontal cortex of both groups of rats. After ethanol withdrawal, hippocampal levels of mGlu1 receptors were higher in unstressed rats, but lower in PRS rats, whereas NPY and CRH levels were similar in the two groups of rats. These data indicate that early life stress has a strong impact on the vulnerability and responsiveness to ethanol consumption during adolescence.
authors.affiliation: NeuroPlasticity Team, UMR 8576, Functional and Structural Glycobiology Unit, CNRS/University Lille North of France, 59655 Villeneuve d'Ascq, France.
authors.forename: V
authors.initials: V
authors.lastname: Van Waes
authors.forename: M
authors.initials: M
authors.lastname: Darnaudéry
authors.forename: J
authors.initials: J
authors.lastname: Marrocco
authors.forename: S H
authors.initials: SH
authors.lastname: Gruber
authors.forename: E
authors.initials: E
authors.lastname: Talavera
authors.forename: J
authors.initials: J
authors.lastname: Mairesse
authors.forename: G
authors.initials: G
authors.lastname: Van Camp
authors.forename: B
authors.initials: B
authors.lastname: Casolla
authors.forename: F
authors.initials: F
authors.lastname: Nicoletti
authors.forename: A A
authors.initials: AA
authors.lastname: Mathé
authors.forename: S
authors.initials: S
authors.lastname: Maccari
authors.forename: S
authors.initials: S
authors.lastname: Morley-Fletcher
mesh_terms: D000428:Alcohol Drinking / Q000503:physiopathology*; D000818:Animals; D002755:Choice Behavior / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006624:Hippocampus / Q000378:metabolism; D046353:Immobility Response, Tonic / Q000187:drug effects / Q000502:physiology; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism; D017397:Prefrontal Cortex / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000503:physiopathology* / Q000523:psychology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism; D012934:Social Isolation / Q000523:psychology; D013312:Stress, Physiological / Q000187:drug effects* / Q000502:physiology*
chemical_list: D009478:Neuropeptide Y; D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1; D000431:Ethanol; D003346:Corticotropin-Releasing Hormone