title: Overexpression of SGR results in oxidative stress and lesion-mimic cell death in rice seedlings.
abstract: It is thought that the Stay Green Rice (SGR) gene is involved in the disaggregation of the light harvesting complex and in the subsequent breakdown of chlorophyll and apo-protein during senescence. In this study, we found that overexpression of SGR (Ov-SGR) resulted in the generation of singlet oxygen and other reactive oxygen species and produced a chlorophyll-dependent regional cell death phenotype on leaves of rice seedlings. Transcriptome analyses using Affymetrix Rice GeneChips revealed that Ov-SGR rice seedlings exhibited a number of signs of singlet oxygen response. The genes and their associated biochemical pathways identified provide an insight into how rice plants respond to singlet oxygen at the molecular and physiologic level.
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Huawu
authors.initials: H
authors.lastname: Jiang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Chen
authors.forename: Meiru
authors.initials: M
authors.lastname: Li
authors.forename: Xinglan
authors.initials: X
authors.lastname: Xu
authors.forename: Guojiang
authors.initials: G
authors.lastname: Wu
mesh_terms: D016923:Cell Death; D002734:Chlorophyll / Q000378:metabolism; D020869:Gene Expression Profiling; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D026082:Singlet Oxygen / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D002734:Chlorophyll; D026082:Singlet Oxygen