title: Examining MRI contrast in three-dimensional cell culture phantoms with DNA-templated nanoparticle chains.
abstract: DNA-templated nanoparticle (NP) chains were examined as potential magnetic resonance imaging (MRI) contrast agents using in vitro environments of the extracellular matrix and tissue. A 3-T clinical MRI scanner was utilized to examine and compare image contrast enhanced by dispersed NPs, DNA-templated NP chains, gold-superparamagnetic multicomponent NP chains, and polyelectrolyte encapsulated, multicomponent NP chains in both T(1)-weighted and T(2)-weighted images. In addition, the longitudinal and transverse relaxivity (r(1) and r(2)) changes were measured both in the basement membrane, using Matrigel, and in the tissue environment, using in vitro 3D cell culture scaffolds. Results suggest that MRI contrast was significantly enhanced from NP chains compared to dispersed NPs in the basement membrane and polyelectrolyte encapsulation for NP chains produced similar relaxivity to nonencapsulated NP chains due to the enhanced cell uptake of encapsulated NP chains.
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47906, USA.
authors.forename: Hamsa
authors.initials: H
authors.lastname: Jaganathan
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Hugar
authors.forename: Albena
authors.initials: A
authors.lastname: Ivanisevic
mesh_terms: D002460:Cell Line; D003094:Collagen; D003287:Contrast Media / Q000737:chemistry* / Q000378:metabolism*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004338:Drug Combinations; D006801:Humans; D007797:Laminin; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009924:Organ Culture Techniques; D011509:Proteoglycans
chemical_list: D003287:Contrast Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D004247:DNA