title: Clinicopathologic significance of fascin, extracellular matrix metalloproteinase inducer, and ezrin expressions in colorectal adenocarcinoma.
abstract: BACKGROUND
  The over expression of fascin, extracellular matrix metalloproteinase inducer (EMMPRIN), and ezrin proteins has been associated with poor prognosis in various carcinomas and sarcomas. However, very few studies have reported the relationship between the expression of fascin, EMMPRIN, and ezrin proteins and the clinico-pathologic parameters of colorectal carcinomas.
  
  
  OBJECTIVE
  The aim was to investigate the relationship between fascin, EMMPRIN, and ezrin proteins in colorectal adenocarcinomas and their correlation with clinico-pathologic parameters.
  
  
  METHODS
  The expression of fascin, EMMPRIN, and ezrin proteins was studied in 210 colorectal adenocarcinoma patients through immunohistochemical staining.
  
  
  METHODS
  Immunohistochemical staining by the avidin-biotin peroxidase method was done. The scoring of each protein expression was done and divided into three groups (negative, low-, and high-expression groups).
  
  
  METHODS
  A chi-square test, and Kendall's tau-b correlation test were used for comparing. Survival analysis was performed using the Kaplan-Meier method with log-rank tests and the Cox proportional hazard model.
  
  
  RESULTS
  The percentages of the high-expression group of fascin, EMMPRIN, and ezrin proteins in colorectal adenocarcinomas were 24%, 73%, and 62%, respectively. Weak positive correlations were observed among these protein expressions. An increased expression of the fascin protein was significantly associated with advanced tumor depth and shorter survival times, and a high expression of fascin protein was an independent prognostic factor in univariate and multivariate survival analyses. EMMPRIN and ezrin protein expressions were not associated with the clinico-pathologic parameters.
  
  
  CONCLUSIONS
  The high expression of fascin protein may be an unfavorable prognostic marker for individual colorectal cancer patients.
authors.affiliation: Department of Pathology, Korea University College of Medicine, Seoul, South Korea.
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Jung
authors.forename: Ju-Han
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Min
authors.forename: Young-Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Jong Sang
authors.initials: JS
authors.lastname: Choi
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051926:Basigin / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000032:analysis*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis*; D008875:Middle Aged; D011379:Prognosis; D055815:Young Adult; D000099149:Ezrin
chemical_list: D051926:Basigin; D015415:Biomarkers; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D000099149:Ezrin; C020324:fascin