title: Cytotoxic effect of hyperthermia and chemotherapy with platinum salt on ovarian cancer cells: results of an in vitro study.
abstract: OBJECTIVE
  Hyperthermic intraperitoneal chemotherapy is continuously under evaluation in ovarian cancer. The purpose of the present study was to evaluate the effect of chemotherapy, drug concentration and temperature.
  
  
  METHODS
  A human ovarian carcinoma cell line was used. The effect of hyperthermia combined with chemotherapy was analyzed.
  
  
  RESULTS
  When hyperthermia was combined with chemotherapy, the 50% lethal dose (LD(50)) decreased with the duration of exposure. The effect of temperature was similar between 39 and 43 °C for a 30-min exposure. For a 60- to 90-min exposure, the LD(50) was equivalent between 38 and 43 °C. Beyond 40 °C, an increase in platinum salt concentration was necessary to obtain similar results according to the duration of exposure.
  
  
  CONCLUSIONS
  The cytotoxic effect of the combination seemed to be potentiated and limited at 40 °C.
authors.affiliation: Unit of Gynecological and Mammary Oncologic Surgery, Centre Hospitalier Universitaire Augustin Morvan, Brest, France. matthieu.muller@chu-brest.fr
authors.forename: Matthieu
authors.initials: M
authors.lastname: Muller
authors.forename: Michel
authors.initials: M
authors.lastname: Chérel
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Dupré
authors.forename: Sébastien
authors.initials: S
authors.lastname: Gouard
authors.forename: Michel
authors.initials: M
authors.lastname: Collet
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Classe
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D016190:Carboplatin / Q000008:administration & dosage / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000628:therapy*
chemical_list: D000970:Antineoplastic Agents; D016190:Carboplatin; D002945:Cisplatin