title: Expression of Bone Morphogenetic Protein-1 in vaginal tissue of women with severe pelvic organ prolapse.
abstract: OBJECTIVE
  To analyze the differential gene and protein expression of Bone Morphogenetic Protein-1 in vaginal tissue of women with advanced pelvic organ prolapse and controls.
  
  
  METHODS
  We sampled the anterior vaginal wall of 39 premenopausal (23 patients and 16 controls), and 18 postmenopausal women (13 patients and 5 controls) during hysterectomy. Total mRNAs and proteins were quantified by real-time RT-PCR and immunoblotting.
  
  
  RESULTS
  Bone Morphogenetic Protein-1 gene expression was decreased in pre- and postmenopausal pelvic organ prolapse patients compared with asymptomatic women (P = .01). The expression of 130 kDa, 92.5 kDa, and 82.5 kDa isoforms of Bone Morphogenetic Protein-1 were down-regulated in postmenopausal patients (P = .01), whereas the 130 kDa isoform expression was up-regulated in premenopausal patients (P = .009), when compared with respective controls.
  
  
  CONCLUSIONS
  The Bone Morphogenetic Protein-1 expression in human vagina was altered in patients with severe pelvic organ prolapse and influenced by menopausal status. Dysregulation of Bone Morphogenetic Protein-1 may contribute for a deficient vaginal connective tissue and support.
authors.affiliation: Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, Mount Sinai Hospital, University of Toronto, Canada. maria.augusta@gmail.com
authors.forename: Maria A T
authors.initials: MA
authors.lastname: Bortolini
authors.forename: Oksana
authors.initials: O
authors.lastname: Shynlova
authors.forename: Harold P
authors.initials: HP
authors.lastname: Drutz
authors.forename: Manoel J B C
authors.initials: MJ
authors.lastname: Girão
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Castro
authors.forename: Stephen
authors.initials: S
authors.lastname: Lye
authors.forename: May
authors.initials: M
authors.lastname: Alarab
mesh_terms: D000328:Adult; D055395:Bone Morphogenetic Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000378:metabolism*; D012720:Severity of Illness Index; D014621:Vagina / Q000378:metabolism*
chemical_list: D055395:Bone Morphogenetic Protein 1