title: Gene sequencing and tissue expression of unknown isoforms of an angiotensin II type 2 receptor interacting protein, ATIP, in the rat.
abstract: To investigate the expression of the unknown angiotensin II type 2 receptor interacting protein (ATIP) isoforms in the rat we used the known sequences of human and mouse ATIP to design sequencing primers to enable us to sequence rat ATIP3 and ATIP4. Exon 4, which is present in human but not mouse ATIP, was not identified in the coding region of rat ATIP. The expression levels of these genes in a range of rat tissues were examined, and we concluded that there is little similarity in the relative tissue distribution of the various ATIP isoforms in rat and human.
authors.affiliation: Clinical Pharmacology and Therapeutics Unit, University of Melbourne, Department of Medicine, Austin Health, Heidelberg, Victoria, Australia.
authors.forename: Michael Angus
authors.initials: MA
authors.lastname: Krezel
authors.forename: Linda Adriana
authors.initials: LA
authors.lastname: Rezmann
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Varghayee
authors.forename: Josefa
authors.initials: J
authors.lastname: Pete
authors.forename: Albert George
authors.initials: AG
authors.lastname: Frauman
authors.forename: Simon Nicholas Stewart
authors.initials: SN
authors.lastname: Louis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D005091:Exons; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D044139:Receptor, Angiotensin, Type 2 / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; C560086:Mtus1 protein, rat; D020033:Protein Isoforms; D012333:RNA, Messenger; D044139:Receptor, Angiotensin, Type 2; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins