title: Discovery, identification and comparative analysis of non-specific lipid transfer protein (nsLtp) family in Solanaceae.
abstract: Plant non-specific lipid transfer proteins (nsLtps) have been reported to be involved in plant defense activity against bacterial and fungal pathogens. In this study, we identified 135 (122 putative and 13 previously identified) Solanaceae nsLtps, which are clustered into 8 different groups. By comparing with Boutrot's nsLtp classification, we classified these eight groups into five types (I, II, IV, IX and X). We compared Solanaceae nsLtps with Arabi-dopsis and Gramineae nsLtps and found that (1) Types I, II and IV are shared by Solanaceae, Gramineae and Arabidopsis; (2) Types III, V, VI and VIII are shared by Gramineae and Arabidopsis but not detected in Solanaceae so far; (3) Type VII is only found in Gramineae whereas type IX is present only in Arabidopsis and Solanaceae; (4) Type X is a new type that accounts for 52.59% Solanaceae nsLtps in our data, and has not been reported in any other plant so far. We further built and compared the three-dimensional structures of the eight groups, and found that the major functional diversification within the nsLtp family could be predated to the monocot/dicot divergence, and many gene duplications and sequence variations had happened in the nsLtp family after the monocot/dicot divergence, especially in Solanaceae.
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China.
authors.forename: Wanfei
authors.initials: W
authors.lastname: Liu
authors.forename: Dawei
authors.initials: D
authors.lastname: Huang
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Gang
authors.initials: G
authors.lastname: Gao
authors.forename: Shuhui
authors.initials: S
authors.lastname: Song
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D002352:Carrier Proteins / Q000145:classification / Q000235:genetics*; D057225:Data Mining; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D014644:Genetic Variation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D006109:Poaceae / Q000235:genetics; D016415:Sequence Alignment; D019657:Solanaceae / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; C072087:sterol carrier proteins