title: Hypoxia-inducible adrenomedullin in colorectal cancer.
abstract: BACKGROUND
  Recently, we determined several potential prognostic factors in vivo using hypoxic tumor cells from hepatic metastases of colorectal cancer (CRC). Among them, expression of adrenomedullin (ADM) was an interesting target because it is highly induced by hypoxia.
  
  
  METHODS
  To evaluate the prognostic impact of the expression of ADM, samples from a total of 373 CRC patients were analyzed by microarray (n = 222), and quantitative reverse-transcriptase polymerase chain reaction (n = 151).
  
  
  RESULTS
  ADM was a novel independent prognostic factor for CRC (p = 0.027). ADM expression correlated with hypoxia-inducible factor-1 A (p < 0.0001) and vascular endothelial growth factor (p < 0.0001) in vivo.
  
  
  CONCLUSIONS
  ADM expression is a useful marker for predicting high risk of relapse and cancer-related death in patients who have undergone curative resection for CRC.
authors.affiliation: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan. hyamamoto@gesurg.med.osaka-u.ac.jp
authors.forename: Mamoru
authors.initials: M
authors.lastname: Uemura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Mizushima
authors.forename: Masataka
authors.initials: M
authors.lastname: Ikeda
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Sekimoto
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D053607:Adrenomedullin / Q000096:biosynthesis* / Q000235:genetics; D000368:Aged; D015687:Cell Hypoxia / Q000502:physiology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
chemical_list: D051793:Hypoxia-Inducible Factor 1; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D053607:Adrenomedullin