title: Influence of low dose irradiation on differentiation, maturation and T-cell activation of human dendritic cells.
abstract: Ionizing irradiation could act directly on immune cells and may induce bystander effects mediated by soluble factors that are released by the irradiated cells. This is the first study analyzing both the direct effect of low dose ionizing radiation (LDIR) on the maturation and cytokine release of human dendritic cells (DCs) and the functional consequences for co-cultured T-cells. We showed that irradiation of DC-precursors in vitro does not influence surface marker expression or cytokine profile of immature DCs nor of mature DCs after LPS treatment. There was no difference of single dose irradiation versus fractionated irradiation protocols on the behavior of the mature DCs. Further, the low dose irradiation did not change the capacity of the DCs to stimulate T-cell proliferation. But the irradiation of the co-culture of DCs and T-cells revealed significantly lower proliferation of T-cells with higher doses. Summarizing the data from approx. 50 DC preparations there is no significant effect of low dose ionizing irradiation on the cytokine profile, surface marker expression and maturation of DCs in vitro although functional consequences cannot be excluded.
authors.affiliation: Department of Radiotherapy and Radiation Oncology, University of Leipzig, Stephanstrasse 21, 04103 Leipzig, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Jahns
authors.forename: Ulf
authors.initials: U
authors.lastname: Anderegg
authors.forename: Anja
authors.initials: A
authors.lastname: Saalbach
authors.forename: Britt
authors.initials: B
authors.lastname: Rosin
authors.forename: Ina
authors.initials: I
authors.lastname: Patties
authors.forename: Annegret
authors.initials: A
authors.lastname: Glasow
authors.forename: Manja
authors.initials: M
authors.lastname: Kamprad
authors.forename: Markus
authors.initials: M
authors.lastname: Scholz
authors.forename: Guido
authors.initials: G
authors.lastname: Hildebrandt
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000528:radiation effects*; D006801:Humans; D008213:Lymphocyte Activation / Q000528:radiation effects*; D011829:Radiation Dosage; D013601:T-Lymphocytes / Q000528:radiation effects*; D014965:X-Rays