title: Quantification of sub-nanomolar levels of Penicillin G by differential pulse adsorptive stripping voltammetry.
abstract: A novel selective and sensitive method is developed for determination of Penicillin G by Differential Pulse Adsorptive Stripping Voltammetry (DPAdSV). Penicillin G gave well-resolved diffusion-controlled cathodic peaks at -0.42 and -0.584 V, respectively (vs Ag/AgCl) in pH 7.50 of borate buffer. Optimal conditions were obtained as pH 7.50, accumulation potential of -0.2 V (vs Ag/AgCl), accumulation time of 120 s, and scan rate of 100 mV/s. Under the optimized conditions, a linear calibration curve was established for the concentration of Penicillin G in the range of 0.007-2.13 µg/ml with a detection limit of 0.000717 µg/ml. The procedure was successfully applied to the determination of Penicillin G in various medicine and biological samples. The relative standard deviation of the method at 0.05 and 0.5 µg/ml Penicillin G, for 10 runs, was 2.55% and 2.06%, respectively.
authors.affiliation: Department of Chemistry, Ilam University, Ilam, Iran. s.abbasi@mail.ilam.ac.ir
authors.forename: S
authors.initials: S
authors.lastname: Abbasi
authors.forename: K
authors.initials: K
authors.lastname: Khodarahmian
authors.forename: A
authors.initials: A
authors.lastname: Farmany
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000652:urine; D002138:Calibration; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006801:Humans; D057230:Limit of Detection; D010400:Penicillin G / Q000032:analysis* / Q000652:urine; D004364:Pharmaceutical Preparations / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D010400:Penicillin G