title: Utilization of real-time PCR to detect Rangifer Cornu contamination in Cervi Parvum Cornu.
abstract: Cervi parvum cornu (CPC) is a well-known ethnopharmacological source, whereas Rangifer cornu (RC) is not considered to be a major source. CPC is distributed in sliced form. Addition of RC to CPC has become an issue in CPC distribution because the appearance of sliced RC is not different from sliced CPC. Therefore, a real-time polymerase chain reaction (PCR) method was developed in this study to detect contaminating RC in CPC. The C-VIC and R-FAM primer/probe sets were designed to specifically amplify CPC and RC DNA, respectively. The specificities and sensitivities of real-time PCR using two primer/probe sets and the applicability of the real-time PCR to powder mixtures, which involved mixtures of powdered CPC and powdered RC in diverse ratios, were evaluated. Real-time PCR using C-VIC and R-FAM primer/probe sets specifically and sensitively amplified both CPC and RC DNA. Furthermore, real-time RCR sensitively detected RC DNA in the powder mixtures of CPC and RC. These results indicate that this real-time PCR method using two primer/probe sets is sufficiently applicable for the detection of contaminant RC in CPC.
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Cheonwon, Korea. justman@korea.kr
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Shim
authors.forename: Rack Seon
authors.initials: RS
authors.lastname: Seong
authors.forename: Dong Sup
authors.initials: DS
authors.lastname: Kim
authors.forename: Shin Jung
authors.initials: SJ
authors.lastname: Kang
authors.forename: Seung Yeup
authors.initials: SY
authors.lastname: Chang
authors.forename: Hyoung Jin
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000999:Antlers; D001688:Biological Products / Q000032:analysis* / Q000235:genetics; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D003670:Deer / Q000145:classification; D004340:Drug Contamination; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001688:Biological Products; D017931:DNA Primers; D004247:DNA