title: Development of a new HPLC method with precolumn fluorescent derivatization for rapid, selective and sensitive detection of triterpenic acids in fruits.
abstract: Triterpenic acids are widespread in plants and have multiplicity of biological properties. Unfortunately the method for accurate analysis of these compounds remains poorly investigated. This study proposed a highly sensitive and selective precolumn derivatization method for accurate determination of five triterpenic acids (betulinic acid, betulonic acid, maslinic acid, ursolic acid and oleanolic acid) in fruits using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescent labeling reagent by HPLC with fluorescence detection (FLD). Response surface methodology was employed to optimize the derivatization reaction, ensuring the sufficient labeling of the analyzed components. The rapid separation of five triterpenic acids could be achieved in as little as 16 min. This developed method offered the exciting detection limits of 1.68-2.04 ng/mL. When applied to several popular fruits in China, it revealed satisfactory applicability and reproducibility. This developed method also exhibits powerful potential for accurate detection of triterpenic acids from other foodstuffs and nature products.
authors.affiliation: Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, 810001, People's Republic of China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Li
authors.forename: Jin-Mao
authors.initials: JM
authors.lastname: You
authors.forename: Cui-Hua
authors.initials: CH
authors.lastname: Song
authors.forename: Lian
authors.initials: L
authors.lastname: Xia
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: You-Rui
authors.initials: YR
authors.lastname: Suo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000032:analysis; D053978:Pentacyclic Triterpenes; D014315:Triterpenes / Q000032:analysis*; D000094062:Betulinic Acid; D000097245:Ursolic Acid
chemical_list: D005456:Fluorescent Dyes; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid; C118308:betulonic acid; C412811:maslinic acid