title: Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales.
abstract: Tributyltin-binding protein type 1 (TBT-bp1) is a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1 (rTBT-bp1, ca. 35kDa) in a baculovirus expression system and purified the protein from the hemolymph of silkworm larvae injected with recombinant baculovirus. After incubation of a mixture of rTBT-bp1 and TBT and its fractionation by means of gel filtration chromatography, TBT was detected in the elution peak of rTBT-bp1, confirming the binding potential of rTBT-bp1 for TBT. An assay of the ability of rTBT-bp1 or native TBT-bp1 (nTBT-bp1) to restore osteoblastic activity inhibited by TBT showed that co-treatment of the scales with rTBT-bp1 or nTBT-bp1 in combination with TBT restored osteoblastic activity in goldfish scales, whereas treatment with TBT alone significantly inhibited osteoblastic activity. These results suggest that TBT-bp1 as a lipocalin member might function to decrease the toxicity of TBT by binding to TBT.
authors.affiliation: Laboratory of Marine Environmental Science, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Hina
authors.initials: H
authors.lastname: Satone
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.forename: Yumi
authors.initials: Y
authors.lastname: Oba
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
authors.forename: Eriko
authors.initials: E
authors.lastname: Akahoshi
authors.forename: Shizuho
authors.initials: S
authors.lastname: Miki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sasayama
authors.forename: Mohamed
authors.initials: M
authors.lastname: Nassef
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.forename: Shun-Ichiro
authors.initials: S
authors.lastname: Kawabata
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Honjo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity; D029941:Fish Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D054834:Lipocalins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D029941:Fish Proteins; D054834:Lipocalins; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin