title: HTK solution helps to preserve endothelial integrity of saphenous vein: an immunohistochemical and ultrastructural analysis.
abstract: The aim of the study is to demonstrate the ability of HTK (Histidine-tryptophan-ketoglutarate) solution to preserve endothelium. Ten saphenous veins (SVs) were prospectively collected from 10 patients who underwent coronary artery bypass grafting (CABG). The SVs were divided into two sets of segments, one of which preserved in HTK solution at 4°C (group A), and the other preserved at 4°C in saline solution NaCl 0.9% (group B); ten pieces from the SVs were processed as control. The control sample was fixed in 10% neutral buffered formalin immediately after harvesting. The observation lasted up to the 5th postoperative day. A morphological, ultrastructural, and immunohistochemical analysis (CD31) was performed on each piece. Immunohistochemical analysis demonstrated significant protection on endothelium in group A compared to group B starting from the 1st observational day. Ultrastructural data confirmed immunohistochemistry. These preliminary results represent a basis for further analysis. They suggest the protective role of HTK solution in preserving endothelial integrity and may imply some clinical benefits in organ protection.
authors.affiliation: Cattedra di Cardiochirurgia, University of Chieti, Pescara, Chieti, Italy.
authors.forename: J
authors.initials: J
authors.lastname: Pizzicannella
authors.forename: R
authors.initials: R
authors.lastname: Rabozzi
authors.forename: O
authors.initials: O
authors.lastname: Trubiani
authors.forename: G
authors.initials: G
authors.lastname: Di Giammarco
mesh_terms: D000368:Aged; D004730:Endothelium, Vascular / Q000166:cytology*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D012501:Saphenous Vein / Q000166:cytology*; D013997:Time Factors
chemical_list: C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose