title: Formation of near-infrared fluorescent nanoparticles for medical imaging.
abstract: OBJECTIVE
  Indocyanine green (ICG) is a US FDA-approved near-infrared fluorescent, water-soluble dye used for diagnostics in vitro and in vivo. The aim of this study was to develop insoluble nanoparticles based on a cationic polymer, ICG and a targeting molecule. The particles are intended for oral administration in the colon, having fluorescence in near-infrared, thus enabling remote detection.
  
  
  METHODS
  An aqueous dispersion of particles formed from Eudragit-RS by simple precipitation method possessing a mean size of approximately 100 nm and zeta potential of +16 mV was produced.
  
  
  RESULTS
  These particles are capable of binding both ICG and fluorescein isothiocyanate-IgG via noncovalent interactions. These composite particles retain the emission characteristics of the fluorescent precursors and also exhibit potential specific recognition ability. The particles were stable in intestinal fluid and are composed only of materials that are FDA approved.
  
  
  CONCLUSIONS
  The nanoparticles may be suitable for in vivo imaging and therapy by oral delivery systems.
authors.affiliation: Casali Institute of Applied Chemistry, Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Liraz
authors.initials: L
authors.lastname: Larush
authors.forename: Shlomo
authors.initials: S
authors.lastname: Magdassi
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D007208:Indocyanine Green / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011109:Polymethacrylic Acids / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D018796:Immunoconjugates; D007074:Immunoglobulin G; D011109:Polymethacrylic Acids; C038300:methylmethacrylate-methacrylic acid copolymer; D007208:Indocyanine Green