title: [Improvement on PCR-CTPP: a SNP genotyping approach based on mismatch technique].
abstract: To explore the technique principle of PCR with confronting two-pair primers (PCR-CTPP) and improve the accuracy of SNP genotyping by taking the 1298 locus of human gene MTHFR as an example, the reliability between conventional PCR-CTPP and improved PCR-CTPP was compared using reconstructed PCR-CTPP detecting system in terms of designing appropriate primers and optimizing annealing temperature and the final concentration of primers. The improved PCR-CTPP detection system proved to be more accurate, which supported the viewpoint on the theoretical defects of conventional PCR-CTPP. The large-scale study verified the reliability of the improved method. It is expected that this improved technique would be widely used in the field of medicine and molecular biology.
authors.affiliation: Henan Key Laboratory of Tumor Epidemiology, Zhengzhou 450052, China. wangke201984@163.com
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Zhang
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Yun
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Wu
authors.forename: A-Qun
authors.initials: AQ
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Kai-Juan
authors.initials: KJ
authors.lastname: Wang
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Dai
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D042965:Methylenetetrahydrofolate Reductase (NADPH2)