title: Novel peptidoglycan-based diagnostic devices for detection of wound infection.
abstract: Detection of wound infection is based on evaluation of the well-known signs of inflammation like rubor (redness), calor (heat), tumor (swelling), and dolor (pain) by medical doctors and/or time-consuming procedures requiring special machinery. There is currently no rapid diagnostic device available for the indication of wound infection, which would especially be helpful in home care of chronic ulcer patients. In this study, a new concept for a fast diagnostic tool for wound infection based on lysozyme and elastase triggered release of dye from a peptidoglycan matrix was investigated. The matrix consisted of alginate/agarose and peptidoglycan covalently labeled with Remazol brilliant blue. Lysozyme activity in postoperative wounds and decubitus wound fluids was significantly elevated upon infection (4830 ± 1848 U mL(-1)) compared to noninfected wounds (376 ± 240 U mL(-1)). Consequently, incubation of 8% (w/v) labeled agarose/peptidoglycan blend layers with infected wound fluid samples for 2 h at 37 °C resulted in a 4-fold higher amount of dye released than measured for noninfected wounds. For alginate/peptidoglycan beads, a 7-fold higher amount of dye was released in case of infected wound fluid samples compared to noninfected ones. Apart from lysozyme, proteases [i.e., gelatinase matrix metalloproteinase MMP-2 and MMP-9 and elastase] were detected in wound fluids (e.g., using Western blotting). When dosed in ratios typical for wounds, a slight synergistic effect was measured for peptidoglycan hydrolysis (i.e., dye release) between lysozyme and these proteases. Incubation of a double-layer system consisting of stained and nonstained peptidoglycan with infected wound fluids resulted in a color change from yellow to blue, thus allowing simple visual detection of wound infection.
authors.affiliation: Department of Environmental Biotechnology, Graz University of Technology, 8010 Graz, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Hasmann
authors.forename: Eva
authors.initials: E
authors.lastname: Wehrschuetz-Sigl
authors.forename: Gertraud
authors.initials: G
authors.lastname: Kanzler
authors.forename: Ulrike
authors.initials: U
authors.lastname: Gewessler
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Hulla
authors.forename: Konstantin P
authors.initials: KP
authors.lastname: Schneider
authors.forename: Barbara
authors.initials: B
authors.lastname: Binder
authors.forename: Michael
authors.initials: M
authors.lastname: Schintler
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D001826:Body Fluids / Q000201:enzymology; D004796:Clinical Enzyme Tests / Q000295:instrumentation / Q000379:methods*; D004396:Coloring Agents / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism*; D014946:Wound Infection / Q000175:diagnosis*
chemical_list: D004396:Coloring Agents; D010457:Peptidoglycan; D009113:Muramidase; D010447:Peptide Hydrolases; D018093:Gelatinases