title: Advancements in reprogramming strategies for the generation of induced pluripotent stem cells.
abstract: Direct reprogramming of somatic cells into induced pluripotent stem (iPS) cells has emerged as an invaluable method for generating patient-specific stem cells of any lineage without the use of embryonic materials. Following the first reported generation of iPS cells from murine fibroblasts using retroviral transduction of a defined set of transcription factors, various new strategies have been developed to improve and refine the reprogramming technology. Recent developments provide optimism that the generation of safe iPS cells without any genomic modification could be derived in the near future for the use in clinical settings. This review summarizes current and evolving strategies in the generation of iPS cells, including types of somatic cells for reprogramming, variations of reprogramming genes, reprogramming methods, and how the advancement iPS cells technology can lead to the future success of reproductive medicine.
authors.affiliation: Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400, Serdang UPM, Selangor, Malaysia.
authors.forename: Mei I
authors.initials: MI
authors.lastname: Lai
authors.forename: Wai Yeng
authors.initials: WY
authors.lastname: Wendy-Yeo
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ramasamy
authors.forename: Norshariza
authors.initials: N
authors.lastname: Nordin
authors.forename: Rozita
authors.initials: R
authors.lastname: Rosli
authors.forename: Abhi
authors.initials: A
authors.lastname: Veerakumarasivam
authors.forename: Syahril
authors.initials: S
authors.lastname: Abdullah
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003001:Cloning, Molecular; D005347:Fibroblasts / Q000166:cytology; D015321:Gene Rearrangement; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D012099:Reproductive Techniques; D012190:Retroviridae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods; D014162:Transfection / Q000379:methods*
chemical_list: D035683:MicroRNAs; D014157:Transcription Factors