title: Nanoarray-based biomolecular detection using individual Au nanoparticles with minimized localized surface plasmon resonance variations.
abstract: Here, we present a mean to expand the use of individual metallic nanoparticles to two-dimensional plasmonic nanoarrays. An optical detection platform to track down localized surface plasmon resonance (LSPR) signals of individual nanoparticles on substrates was built for the application of plasmonic nanoarrays. A pseudoimage of nanoparticles on a substrate was reconstructed from their scattering spectra obtained by scanning a user-defined area. The spectral and spatial resolutions of the system were also discussed in detail. Most importantly, we present a method to normalize the localized surface plasmon resonance from geometrically different nanoparticles. After normalization, plasmonic responses from different particles become highly consistent, creating well-fitted dose-response curves of both surrounding refractive index changes and receptor-analyte binding to the surface of individual nanoparticles. Finally, the proof-of-concept system for plasmonic nanoarray detection is demonstrated by the measurement of the aptamer-thrombin binding event.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore.
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.forename: Abdul Rahim
authors.initials: AR
authors.lastname: Ferhan
authors.forename: Kijoon
authors.initials: K
authors.lastname: Lee
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kim
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013917:Thrombin / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D013917:Thrombin