title: Preparation of lyophilized cells to preserve enzyme activities and high molecular weight nucleic acids.
abstract: This procedure yields thin flakes of freeze-dried material from tissue culture cells. Up to 0.5 g of wet cells can be processed at one time. Dried cells, stored indefinitely at -20°C, have full lactate dehydrogenase activity (1) and DNA polymerase activity (2). The dried cells stored for a few days at room temperature also have apparently undegraded nucleic acids (3).
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, Utah.
authors.forename: T
authors.initials: T
authors.lastname: Gurney