title: Composite polymeric magnetic nanoparticles for co-delivery of hydrophobic and hydrophilic anticancer drugs and MRI imaging for cancer therapy.
abstract: Exercising complementary roles of polymer-coated magnetic nanoparticles for precise drug delivery and image contrast agents has attracted significant attention in biomedical applications. The objective of this study was to prepare and characterize magnetic nanoparticles embedded in polylactide-co-glycolide matrixes (PLGA-MNPs) as a dual drug delivery and imaging system capable of encapsulating both hydrophilic and hydrophobic drugs. PLGA-MNPs were capable of encapsulating both hydrophobic and hydrophilic drugs in a 2:1 ratio. Biocompatibility, cellular uptake, cytotoxicity, membrane potential, and apoptosis were carried out in two different cancer cell lines (MCF-7 and PANC-1). The molecular basis of induction of apoptosis was validated by Western blotting analysis. For targeted delivery of drugs, targeting ligand such as Herceptin was used, and such a conjugated system demonstrated enhanced cellular uptake and an augmented synergistic effect in an in vitro system when compared with native drugs. Magnetic resonance imaging was carried out both in vitro and in vivo to assess the efficacy of PLGA-MNPs as contrast agents. PLGA-MNPs showed a better contrast effect than commercial contrast agents due to higher T(2) relaxivity with a blood circulation half-life ∼ 47 min in the rat model. Thus, our results demonstrated the dual usable purpose of formulated PLGA-MNPs toward either, in therapeutics by delivering different hydrophobic or hydrophilic drugs individually or in combination and imaging for cancer therapeutics in the near future.
authors.affiliation: Laboratory of Nanomedicine, Institute of Life Sciences, Nalco Square, Bhubaneswar -751023, Orissa.
authors.forename: Abhalaxmi
authors.initials: A
authors.lastname: Singh
authors.forename: Fahima
authors.initials: F
authors.lastname: Dilnawaz
authors.forename: Sujeet
authors.initials: S
authors.lastname: Mewar
authors.forename: Uma
authors.initials: U
authors.lastname: Sharma
authors.forename: N R
authors.initials: NR
authors.lastname: Jagannathan
authors.forename: Sanjeeb Kumar
authors.initials: SK
authors.lastname: Sahoo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000138:chemical synthesis; D004337:Drug Carriers / Q000138:chemical synthesis*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000033:anatomy & histology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D004337:Drug Carriers; D058185:Magnetite Nanoparticles