title: Development of a flow-through [corrected] microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of European Bunyaviruses. [corrected].
abstract: It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in Europe. Currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. In order to arrive at an assay system useful for high throughput analysis of samples from aseptic meningo-encephalitis cases the authors developed a combined multiplex ligation-dependent probe amplification and flow-through microarray assay for the detection of European Bunyaviruses. These results show that this combined assay indeed is highly sensitive, and specific for the accurate detection of multiple viruses.
authors.affiliation: Department of Virology, University Medical Center Göttingen, Kreuzbergring 57 37075, Göttingen, Germany. lhasib@gwdg.de
authors.forename: Lekbira
authors.initials: L
authors.lastname: Hasib
authors.forename: Meik
authors.initials: M
authors.lastname: Dilcher
authors.forename: Frank
authors.initials: F
authors.lastname: Hufert
authors.forename: Ursula
authors.initials: U
authors.lastname: Meyer-König
authors.forename: Ursula
authors.initials: U
authors.lastname: König-Meyer
authors.forename: Manfred
authors.initials: M
authors.lastname: Weidmann
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000235:genetics / Q000302:isolation & purification*; D002522:Chlorocebus aethiops; D005060:Europe; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009841:Oligonucleotides; D016851:Orthobunyavirus / Q000235:genetics / Q000302:isolation & purification*; D016856:Phlebovirus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D013696:Temperature; D014709:Vero Cells
chemical_list: D009841:Oligonucleotides; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase