title: Assessment of the protective effects of selected dietary anticarcinogens against DNA damage and cytogenetic effects induced by benzo[a]pyrene in C57BL/6J mice.
abstract: The protective action in C57BL/6J mice from orally administered ellagic acid (EA), benzyl isothiocyanate (BITC), an extract of epigallocatechins (Tegreen®) as well as chlorophyllin (CHL) against benzo[a]pyrene (B[a]P)-induced DNA damage and cytogenetic effects was investigated. In pilot experiment the comet assay indicated protective effects for all compounds, while such activity was confined to EA and CH with respect to B[a]P-DNA adducts and micronuclei. EA and CH were chosen for the main study where the levels of DNA adducts in liver after injection of 30 mg B[a]P/kg b.w. did not differ from those found for animals exposed to B[a]P and treated with the protective substances. In leukocytes no significant protective effect of CHL was detected while a 2-fold increase of adduct concentrations was observed after co-administration of EA. In the comet assay CHL or EA caused a 3-fold decrease of SSB, and a 2-fold decrease of FPG sites in comparison to animals treated with B[a]P. CHL or EA showed a significant protective effect against B[a]P-induced MN in polychromatic erythrocytes in bone marrow. In contrast, flow cytometry measurements in peripheral blood indicated the MN frequency after treatment with CHL or EA almost twice as high as that recorded for B[a]P alone.
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, Łódź, Poland. mstep@imp.lodz.pl
authors.forename: Dobrosława
authors.initials: D
authors.lastname: Gradecka-Meesters
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Palus
authors.forename: Gabriela
authors.initials: G
authors.lastname: Prochazka
authors.forename: Dan
authors.initials: D
authors.lastname: Segerbäck
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Dziubałtowska
authors.forename: Natalia
authors.initials: N
authors.lastname: Kotova
authors.forename: Dag
authors.initials: D
authors.lastname: Jenssen
authors.forename: Joanna
authors.initials: J
authors.lastname: Arkusz
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lundin
authors.forename: Elisabet
authors.initials: E
authors.lastname: Vikström
authors.forename: Konrad
authors.initials: K
authors.lastname: Rydzyński
authors.forename: Robert
authors.initials: R
authors.lastname: Nilsson
authors.forename: Maciej
authors.initials: M
authors.lastname: Stępnik
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001853:Bone Marrow / Q000737:chemistry; D018890:Chemoprevention; D002735:Chlorophyllides / Q000008:administration & dosage / Q000494:pharmacology; D020552:Comet Assay; D003582:Cytogenetics; D018736:DNA Adducts / Q000187:drug effects*; D004610:Ellagic Acid / Q000008:administration & dosage / Q000494:pharmacology; D023381:Endpoint Determination; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D017879:Isothiocyanates / Q000008:administration & dosage / Q000494:pharmacology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests / Q000379:methods; D010865:Pilot Projects
chemical_list: D016588:Anticarcinogenic Agents; D002735:Chlorophyllides; D018736:DNA Adducts; D017879:Isothiocyanates; D004610:Ellagic Acid; D001564:Benzo(a)pyrene; C031403:benzyl isothiocyanate; C007020:chlorophyllin