title: Identification of Streptococcus pneumoniae Cps2C residues that affect capsular polysaccharide polymerization, cell wall ligation, and Cps2D phosphorylation.
abstract: A number of single amino acid substitutions throughout Streptococcus pneumoniae Cps2C were found to affect its function and confer either a mucoid or a small colony phenotype. These mutants exhibit significant changes in capsular polysaccharide (CPS) profile relative to that of wild-type pneumococci. The introduced mutations affect either polymerization or ligation of CPS to the cell wall and/or Cps2D phosphorylation.
authors.affiliation: School of Molecular and Biomedical Science, University of Adelaide, Adelaide, S.A. 5005, Australia.
authors.forename: James P
authors.initials: JP
authors.lastname: Byrne
authors.forename: Judy K
authors.initials: JK
authors.lastname: Morona
authors.forename: James C
authors.initials: JC
authors.lastname: Paton
authors.forename: Renato
authors.initials: R
authors.lastname: Morona
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D016667:Bacterial Capsules / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial