title: [Preparation and application of goat deltafosB gene expression product antibody].
abstract: deltaFosB, a naturally occurring truncated isform of fosB gene, existed in many tissues stably and played an important role in formation and differentiation of adipocyte and osteoblast. deltaFosB may be related to the metabolism of calcium in bone and mammary gland and regulate the signal pathway of calcium transfer from bone to mammary gland. We first sub-cloned deltafosB gene of goat into the vector pET32a to construct prokaryotic expression vector pET32a-deltafosB. Then we induced for deltafosB gene expression efficiently by IPTG. Finally we immunized the adult rabbits with purified recombinant deltaFosB to prepare rabbit anti-goat deltaFosB polyclonal antibody. iELISA analysis showed the antibody with the titer of 1:51 200, and Western blotting result showed that the antibody could specifically detect the deltaFosB protein expressed in prokaryotic cell and HEK-293 cell, respectively. Further Western blotting assay showed that deltaFosB expressed in various tissues of goat in vivo.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Zheng.huiling@yahoo.com
authors.forename: Huiling
authors.initials: H
authors.lastname: Zheng
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Junhui
authors.initials: J
authors.lastname: An
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Yang
authors.forename: Ruifang
authors.initials: R
authors.lastname: Xing
authors.forename: Linhui
authors.initials: L
authors.lastname: Yan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006041:Goats; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016760:Proto-Oncogene Proteins c-fos; D011994:Recombinant Proteins