title: Spermine grafted galactosylated chitosan for improved nanoparticle mediated gene delivery.
abstract: Despite multitude of beneficial features, chitosan has poor water solubility and transfection ability which affect its gene delivery efficacy. The two features are improved when certain chemical modifications are incorporated into the chitosan parent backbone. This strategy is adopted here, by coupling galactose and spermine into the chitosan backbone. The conjugation was determined with FTIR and (1)H NMR and nanoparticle morphology was assessed by TEM and AFM techniques. Particle size, zeta potential, buffering capacity and DNA binding ability gave encouraging result of enhanced solubility and stability. In vitro studies of GCSM in HepG2 cell lines displayed low cytotoxicity and improved transfection. We also identified the preference of receptor mediated internalization for nanoparticles cellular uptake by treating with cellular uptake inhibitors. The results evidently led us to comprehend that galactosylated chitosan-g-spermine could be considered as a promising chitosan derivative for conducting nanoparticle mediated gene delivery.
authors.affiliation: Biosurface Technology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Poojappura, Thiruvananthapuram, Kerala 695012, India.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Alex
authors.forename: M R
authors.initials: MR
authors.lastname: Rekha
authors.forename: Chandra P
authors.initials: CP
authors.lastname: Sharma
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D004355:Drug Stability; D005690:Galactose / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000008:administration & dosage*; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles; D010316:Particle Size; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013096:Spermine / Q000737:chemistry*; D014162:Transfection
chemical_list: D013096:Spermine; D048271:Chitosan; D005690:Galactose