title: Downregulation of caveolin-1 in a murine model of acute allergic airway disease.
abstract: BACKGROUND
  Airway remodeling refers to the structural changes in the airways of asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle cell proliferation. The aim was to investigate lung caveolin-1 status in a murine model of acute allergic airway disease.
  
  
  METHODS
  Six- to eight-week-old female BALB/c mice were sensitized by intraperitoneal injections of phosphate-buffered saline or ovalbumin (OVA) and aluminium hydroxide on Days 0 and 14, challenged with aerosolized saline or OVA (1%) on Days 21-25, 28-32, and 35. The mice were killed 1 day after the last OVA/saline challenge. Serum OVA-specific immunoglobulin E (IgE) was measured by enzyme-linked immunosorbent assay. Peribronchial inflammation was quantified by morphometric analysis. Lung caveolin-1 and Type I collagen mRNA expression was determined by real-time reverse-transcription polymerase chain reaction. Total lung collagen was measured using Sircol Assay Kit.
  
  
  RESULTS
  Serum OVA-specific IgE levels were significantly elevated in OVA-challenged mice when compared with saline-challenged mice. Percentage of inflammatory cells in the bronchoalveolar lavage was significantly higher in the OVA-challenged animals. The animals' lungs that were sensitized and challenged with OVA contained large numbers of inflammatory cells concentrated near the airways and in the perivascular areas. The thickness of the bronchial epithelial layer and smooth muscle layer and the numbers of total inflammatory cells and eosinophils significantly increased in OVA-challenged mice. Caveolin-1 mRNA expression significantly decreased and Type I collagen mRNA expression significantly increased in the lung tissue of OVA-challenged mice.
  
  
  CONCLUSIONS
  These results suggest that caveolin-1 seems to be involved in the pathogenesis of airway remodeling of acute allergic airway disease.
authors.affiliation: Department of Pediatrics, Dajia Lee's Hospital, Lee's Medical Corporation, Taichung, Taiwan.
authors.forename: Chung-Ming
authors.initials: CM
authors.lastname: Chen
authors.forename: Meng-Ying
authors.initials: MY
authors.lastname: Wu
authors.forename: Hsiu-Chu
authors.initials: HC
authors.lastname: Chou
authors.forename: Yaw-Dong
authors.initials: YD
authors.lastname: Lang
authors.forename: Leng-Fang
authors.initials: LF
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D056151:Airway Remodeling; D000818:Animals; D001249:Asthma / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D018893:Bronchoalveolar Lavage; D051242:Caveolin 1 / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D051242:Caveolin 1; D003094:Collagen