title: Antigen-specific IgA B memory cell responses to Shigella antigens elicited in volunteers immunized with live attenuated Shigella flexneri 2a oral vaccine candidates.
abstract: We studied the induction of antigen-specific IgA memory B cells (B(M)) in volunteers who received live attenuated Shigella flexneri 2a vaccines. Subjects ingested a single oral dose of 10(7), 10(8) or 10(9) CFU of S. flexneri 2a with deletions in guaBA (CVD 1204) or in guaBA, set and sen (CVD 1208). Antigen-specific serum and stool antibody responses to LPS and Ipa B were measured on days 0, 7, 14, 28 and 42. IgA B(M) cells specific to LPS, Ipa B and total IgA were assessed on days 0 and 28. We show the induction of significant LPS-specific IgA B(M) cells in anti-LPS IgA seroresponders. Positive correlations were found between anti-LPS IgA B(M) cells and anti-LPS IgA in serum and stool; IgA B(M) cell responses to IpaB were also observed. These B(M) cell responses are likely play an important role in modulating the magnitude and longevity of the humoral response.
authors.affiliation: Division of Infectious Diseases and Tropical Pediatrics, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Simon
authors.forename: M
authors.initials: M
authors.lastname: Maciel
authors.forename: E D
authors.initials: ED
authors.lastname: Weld
authors.forename: R
authors.initials: R
authors.lastname: Wahid
authors.forename: M F
authors.initials: MF
authors.lastname: Pasetti
authors.forename: W L
authors.initials: WL
authors.lastname: Picking
authors.forename: K L
authors.initials: KL
authors.lastname: Kotloff
authors.forename: M M
authors.initials: MM
authors.lastname: Levine
authors.forename: M B
authors.initials: MB
authors.lastname: Sztein
mesh_terms: D000284:Administration, Oral; D000293:Adolescent; D000328:Adult; D000917:Antibody Formation / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology; D005243:Feces / Q000737:chemistry; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D018655:Lymphocyte Count; D008875:Middle Aged; D017384:Sequence Deletion; D022281:Shigella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012763:Shigella flexneri / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*; D055815:Young Adult
chemical_list: D000941:Antigens; D015703:Antigens, CD; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D016023:Integrins; D008070:Lipopolysaccharides; D022281:Shigella Vaccines; D014613:Vaccines, Attenuated; C071777:integrin alpha4beta7; C087447:ipaB protein, Shigella