title: PEGylation prevents bacteria-induced platelet activation and biofilm formation in platelet concentrates.
abstract: Bacterial contamination of platelet concentrates represents the greatest post-transfusion infectious risk. Biofilm formation in this environment resulting from platelet-bacteria interactions can lead to non-uniform contaminant distribution and thus missed detection. As formation of platelet-bacteria aggregates is largely based on receptor-ligand interactions, we examined whether shielding these events would result in reduced biofilm formation by contaminant bacteria. We introduced methoxypoly(ethylene glycol) to covalently modify the platelet surface using a process termed 'PEGylation'. In the first study of its kind, we demonstrate that PEGylated platelet concentrates inoculated with Staphylococcus epidermidis display a significant reduction in bacterial binding and biofilm formation.
authors.affiliation: Canadian Blood Services, Ottawa, Ontario, Canada.
authors.forename: C A
authors.initials: CA
authors.lastname: Greco
authors.forename: E
authors.initials: E
authors.lastname: Maurer-Spurej
authors.forename: M D
authors.initials: MD
authors.lastname: Scott
authors.forename: M
authors.initials: M
authors.lastname: Kalab
authors.forename: N
authors.initials: N
authors.lastname: Nakane
authors.forename: S M
authors.initials: SM
authors.lastname: Ramírez-Arcos
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000472:pathogenicity; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D001792:Blood Platelets / Q000737:chemistry* / Q000382:microbiology; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D015539:Platelet Activation / Q000187:drug effects*; D017713:Platelet Transfusion / Q000592:standards; D011092:Polyethylene Glycols / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol