title: Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample.
abstract: An actinomycete strain, IR73-Li102(T), was isolated from a lichen sample obtained from Iriomote Island, Japan, and subsequently characterized using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain IR73-Li102(T) had the highest sequence similarities with Actinomycetospora chiangmaiensis YIM 0006(T) (98.3%), A. corticola 014-5(T) (98.1%) and A. rishiriensis RI109-Li102(T) (98.0%). However, DNA-DNA hybridization assays, as well as physiological and biochemical analyzes, showed that strain IR73-Li102(T) could be clearly differentiated from its closest phylogenetic relatives. The strain contained meso-diaminopimelic acid, and arabinose and galactose were present in whole-cell hydrolysates. The predominant menaquinone was MK-8(H(4)), and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acid was iso-C(16:0) (58%). The chemotaxonomic properties of strain IR73-Li102(T) were consistent with those shared by members of the genus Actinomycetospora. On the basis of the phenotypic features and DNA-DNA hybridization data, strain IR73-Li102(T) (= NBRC 106365(T) = KCTC 19783(T)) represents a novel species of the genus Actinomycetospora, for which the name Actinomycetospora iriomotensis sp. nov. is proposed.
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Yamanashi, Japan. hyamamura@yamanashi.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.forename: Haruna
authors.initials: H
authors.lastname: Ashizawa
authors.forename: Youji
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.forename: Yuumi
authors.initials: Y
authors.lastname: Ishida
authors.forename: Misa
authors.initials: M
authors.lastname: Otoguro
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial; D007564:Japan; D008011:Lichens / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D017423:Sequence Analysis, RNA
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S