title: Identification of Campylobacter species and related organisms by matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry.
abstract: The identification of Campylobacter species and related organisms at the species level has always been difficult using phenotypic methods because of their low metabolic activity, whereas molecular methods are more reliable but time-consuming. In this study, 1007 different strains were identified using three different methods: conventional methods, molecular biology (real-time PCR and sequencing) and matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Molecular methods were considered the gold standard. The accuracy of MALDI-TOF mass spectrometry reached 100% compared with the gold standard for all of the Campylobacter species, except Campylobacter jejuni (99.4%). The accuracy of conventional methods compared with the gold standard ranged from 0% to 100% depending on the species. However, MALDI-TOF mass spectrometry was not able to identify a mixture of two different species present in the same sample in four instances. Finally, MALDI-TOF mass spectrometry is highly recommended to identify Campylobacter spp. as only 0.4% discrepancy was found, whereas conventional methods led to 4.5% discrepancy.
authors.affiliation: Université Victor Segalen Bordeaux 2, Centre National de Référence des Campylobacters et Hélicobacters, Bordeaux Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Bessède
authors.forename: O
authors.initials: O
authors.lastname: Solecki
authors.forename: E
authors.initials: E
authors.lastname: Sifré
authors.forename: L
authors.initials: L
authors.lastname: Labadi
authors.forename: F
authors.initials: F
authors.lastname: Mégraud
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002167:Campylobacter / Q000737:chemistry* / Q000145:classification*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*