title: High prevalence of extended-spectrum beta lactamases among Salmonella enterica Typhimurium isolates from pediatric patients with diarrhea in China.
abstract: We investigated the extended-spectrum beta lactamases among 62 Salmonella enterica Typhimurium isolates recovered from children with diarrhea in a Chinese pediatric hospital. A large proportion of S. enterica Typhimurium isolates were resistant to multiple antimicrobial agents, including ampicillin (90.3%), tetracycline (80.6%), trimethoprim/sulfamethoxazole (74.2%), chloramphenicol (66.1%), cefotaxime (27.4%). Forty-nine (79.0%) of S. enterica Typhimurium isolates were positive for bla(TEM-1b) and resistant to ampicillin. Thirteen S. enterica Typhimurium isolates (21.0%) were positive for bla(CTX-M-1-group) and bla(CTX-M-9-group), and all isolates harboring bla(CTX-M) genes were positive for ISEcp1. Two main clones (PFGE type A and D) accounted for nearly 70% of S. enterica Typhimurium isolates, and 7 CTX-M-producing isolates belonged to PFGE type D. Collectively, our data reveal multi-drug resistance and a high prevalence of extended spectrum beta lactamases among S. enterica Typhimurium isolates from children in China. In addition, we report the first identification of bla(CTX-M-55) within Salmonella spp. Our data also suggest that clonal spread is responsible for the dissemination of S. enterica Typhimurium isolates.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors.forename: Fangyou
authors.initials: F
authors.lastname: Yu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yu
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Li
authors.forename: Baixing
authors.initials: B
authors.lastname: Ding
authors.forename: Lehe
authors.initials: L
authors.lastname: Yang
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Qin
authors.forename: Chris
authors.initials: C
authors.lastname: Parsons
authors.forename: Xueqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Jinwei
authors.initials: J
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Liangxing
authors.initials: L
authors.lastname: Wang
authors.forename: Jingye
authors.initials: J
authors.lastname: Pan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D002681:China; D003967:Diarrhea / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D019779:Salmonella enterica / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases