title: Chemiluminescence investigations of antioxidative activities of some antibiotics against superoxide anion radical.
abstract: A chemiluminescent technique was applied to determine antioxidative activities of adriamycin, farmorubicin, mitomycin C and bleomycin against superoxide anion radical (O(2)(•)) in aprotic medium. The antioxidant capacity was expressed as the decrease in light emission from the O(2)(•) solution by and antibiotic. A KO(2) solution in dimethyl sulphoxide (DMSO) and 18-crown-6 ether were used for the generation of O(2)(•). The results showed that the examined compounds decreased the chemiluminescence (CL) sum from the O(2)(•)-generating system in a dose-dependent manner. Among the antibiotics examined, adriamycin, farmorubicin and bleomycin exhibited antioxidant activity almost comparable to that of 1,2-dihydroxy benzene-3,5-disulphonic acid (tiron), an efficient of the O(2)(•) inhibitor. Mitomycin C was two-times less effective as tiron in decreasing the initial CL intensity. The proposed assay with usage of ultraweak CL technique and the KO(2)-DMSO-crown ether system was useful for the evaluation of antioxidant activity in aprotic solvents.
authors.affiliation: Institute of Physics, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology in Szczecin, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Kruk
authors.forename: Teresa
authors.initials: T
authors.lastname: Michalska
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kładna
authors.forename: Paweł
authors.initials: P
authors.lastname: Berczyński
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D049449:Luminescence; D013481:Superoxides / Q000037:antagonists & inhibitors*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D013481:Superoxides