title: Validation and diagnostic application of NS and HA gene-specific real-time reverse transcription-PCR assays for detection of 2009 pandemic influenza A (H1N1) viruses in clinical specimens.
abstract: Real-time reverse transcription-PCR assays specific for the nonstructural (NS) and hemagglutinin (HA) genes of the 2009 pandemic influenza A (H1N1) virus were developed and evaluated with clinical samples from infected patients. The tests are characterized by high sensitivity and specificity and performed well throughout the first year of the 2009 pandemic.
authors.affiliation: Viral Infections Unit, Department of Vaccination and Immune Protection, National Institute for Health and Welfare (THL),Helsinki, Finland. esa.ronkko@thl.fi
authors.forename: Esa
authors.initials: E
authors.lastname: Rönkkö
authors.forename: Niina
authors.initials: N
authors.lastname: Ikonen
authors.forename: Mia
authors.initials: M
authors.lastname: Kontio
authors.forename: Minna
authors.initials: M
authors.lastname: Haanpää
authors.forename: Hannimari
authors.initials: H
authors.lastname: Kallio-Kokko
authors.forename: Laura
authors.initials: L
authors.lastname: Mannonen
authors.forename: Maija
authors.initials: M
authors.lastname: Lappalainen
authors.forename: Ilkka
authors.initials: I
authors.lastname: Julkunen
authors.forename: Thedi
authors.initials: T
authors.lastname: Ziegler
mesh_terms: D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014773:Virology / Q000379:methods*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C108309:NS protein, influenza virus; D017361:Viral Nonstructural Proteins