title: Quantitative characterization of planarian wild-type behavior as a platform for screening locomotion phenotypes.
abstract: Changes in animal behavior resulting from genetic or chemical intervention are frequently used for phenotype characterizations. The majority of these studies are qualitative in nature, especially in systems that go beyond the classical model organisms. Here, we introduce a quantitative method to characterize behavior in the freshwater planarian Schmidtea mediterranea. Wild-type locomotion in confinement was quantified using a wide set of parameters, and the influences of intrinsic intra-worm versus inter-worm variability on our measurements was studied. We also examined the effect of substrate, confinement geometry and the interactions with the boundary on planarian behavior. The method is based on a simple experimental setup, using automated center-of-mass tracking and image analysis, making it an easily implemented alternative to current methods for screening planarian locomotion phenotypes. As a proof of principle, two drug-induced behavioral phenotypes were generated to show the capacity of this method.
authors.affiliation: Carl Icahn Laboratory, Lewis-Sigler Institute, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Jared
authors.initials: J
authors.lastname: Talbot
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Schötz
mesh_terms: D000777:Anesthetics / Q000494:pharmacology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002724:Chlorobutanol / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D005838:Genotype; D008124:Locomotion / Q000187:drug effects; D018715:Microscopy, Video / Q000379:methods; D010641:Phenotype; D010932:Planarians / Q000235:genetics / Q000502:physiology*; D013469:Sulpiride / Q000494:pharmacology
chemical_list: D000777:Anesthetics; D018492:Dopamine Antagonists; D013469:Sulpiride; D002724:Chlorobutanol