title: A lentiviral vector pseudotype suitable for vaccine development.
abstract: BACKGROUND
  Lentiviral vectors (LV) are promising vaccines because they transduce dendritic cells (DC) in vivo. To translate LV vaccines into clinical trials, bulk production will be necessary. The present study aimed to find a suitable envelope for LV vaccine production from stable packaging cells because the commonly used vesicular stomatitis virus envelope (VSV-G) is cytotoxic.
  
  
  METHODS
  The envelope from Ross river virus (RRV) was selected. It can infect mouse and human cells, allowing testing in animals before clinical translation. We used VSV-G for comparison. Vectors produced with each envelope were titred on human 293T cells and mouse 3T3 cells.
  
  
  RESULTS
  RRV-pseudotyped LV (RRV-LV) infected mouse myeloid DC in culture and immunized mice. An approximately 50-fold higher dose of RRV-LV than VSV-G-LV was required to generate a similar T cell response. The RRV-LV could also be used to infect human mDC and to prime a human T cell immune response.
  
  
  CONCLUSIONS
  RRV envelope is a suitable candidate to be used for the construction of an LV producer cell line. LV vaccines with RRV envelope can be tested in mice and in human immune cell cultures. The higher dose of RRV-LV required for vaccine efficacy compared to VSV-G-LV will partly be offset by ease of production.
authors.affiliation: Immunity and Infection, University College London, London, UK.
authors.forename: Luciene
authors.initials: L
authors.lastname: Lopes
authors.forename: Marie
authors.initials: M
authors.lastname: Dewannieux
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Mary K
authors.initials: MK
authors.lastname: Collins
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000378:metabolism; D058501:Enzyme-Linked Immunospot Assay; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012398:Ross River virus / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014161:Transduction, Genetic; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity
chemical_list: D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins