title: Gene expression profiling of duodenal biopsies discriminates celiac disease mucosa from normal mucosa.
abstract: Celiac disease (CD) is identified by histopathologic changes in the small intestine which normalize during a gluten-free diet. The histopathologic assessment of duodenal biopsies is usually routine but can be difficult. This study investigated gene expression profiling as a diagnostic tool. A total of 109 genes were selected to reflect alterations in crypt-villi architecture, inflammatory response, and intestinal permeability and were examined for differential expression in normal mucosa compared with CD mucosa in pediatric patients. Biopsies were classified using discriminant analysis of gene expression. Fifty genes were differentially expressed, of which eight (APOC3, CYP3A4, OCLN, MAD2L1, MKI67, CXCL11, IL17A, and CTLA4) discriminated normal mucosa from CD mucosa without classification errors using leave-one-out cross-validation (n = 39) and identified the degree of mucosal damage. Validation using an independent set of biopsies (n = 27) resulted in four discrepant cases. Biopsies from two of these cases showed a patchy distribution of lesions, indicating that discriminant analysis based on single biopsies failed to identify CD mucosa. In the other two cases, serology support class according to discriminant analysis and histologic specimens were judged suboptimal but assessable. Gene expression profiling shows promise as a diagnostic tool and for follow-up of CD, but further evaluation is needed.
authors.affiliation: Division of Medical Diagnostics, Ryhov County Hospital, Jönköping SE-551 85, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Bragde
authors.forename: Ulf
authors.initials: U
authors.lastname: Jansson
authors.forename: Ingvar
authors.initials: I
authors.lastname: Jarlsfelt
authors.forename: Jan
authors.initials: J
authors.lastname: Söderman
mesh_terms: D000293:Adolescent; D001706:Biopsy; D002446:Celiac Disease / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D055050:Diet, Gluten-Free; D016002:Discriminant Analysis; D004386:Duodenum / Q000033:anatomy & histology* / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000473:pathology / Q000502:physiology / Q000503:physiopathology; D008297:Male; D046228:Microarray Analysis