title: A readily synthesized cyclic pyrrolysine analogue for site-specific protein "click" labeling.
abstract: A concise route was developed for the facile synthesis of a cyclic pyrrolysine analogue bearing an azide handle. Directed evolution enabled the encoding of this non-natural amino acid in both prokaryotic and eukaryotic cells, which offers a highly efficient approach for the site-specific protein labeling using click chemistry.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Hao
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Song
authors.forename: Shixian
authors.initials: S
authors.lastname: Lin
authors.forename: Maiyun
authors.initials: M
authors.lastname: Yang
authors.forename: Yujie
authors.initials: Y
authors.lastname: Liang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Peng R
authors.initials: PR
authors.lastname: Chen
mesh_terms: D001665:Binding Sites; D057930:Click Chemistry; D019020:Directed Molecular Evolution; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D008250:Lysine-tRNA Ligase / Q000737:chemistry / Q000235:genetics; D017021:Methanosarcina barkeri / Q000201:enzymology; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D013194:Staining and Labeling; D013379:Substrate Specificity; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D011506:Proteins; D016159:Tumor Suppressor Protein p53; C084501:hdeA protein, E coli; D008250:Lysine-tRNA Ligase; C456839:pyrrolysine; D008239:Lysine