title: Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving.
abstract: The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Building 76-253, Cambridge, MA 02139, USA.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Panagiotou
authors.forename: Kerry Routenberg
authors.initials: KR
authors.lastname: Love
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Juergen
authors.initials: J
authors.lastname: Nett
authors.forename: Terrance
authors.initials: T
authors.lastname: Stadheim
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Love
mesh_terms: D001681:Biological Assay / Q000379:methods; D001709:Biotechnology / Q000379:methods*; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D008403:Mass Screening / Q000379:methods; D016296:Mutagenesis; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D007141:Immunoglobulin Fc Fragments; D011994:Recombinant Proteins