title: Recombinant lectin microarrays for glycomic analysis.
abstract: The cell surface is covered with a myriad of carbohydrates that form a complex matrix of oligosaccharides. Carbohydrate recognition plays critical roles in pathogenesis, trafficking, and differentiation. Lectin microarray technology presents a novel platform for the high-throughput analysis of these structurally diverse biopolymers. One drawback of this technology has been limitations imposed by the commercially available plant lectins used in the array. Not only are a majority of these plant-derived proteins glycosylated, which can complicate glycomic analysis, but they also differ in activity and availability. Our lab has recently introduced recombinant lectins to enhance the stability and scope of our lectin panel. Herein, we provide a detailed procedure for the expression of bacterially-derived lectins and their application to a recombinant lectin microarray.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Propheter
authors.forename: Ku-Lung
authors.initials: KL
authors.lastname: Hsu
authors.forename: Lara K
authors.initials: LK
authors.lastname: Mahal
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D045744:Cell Line, Tumor; D004274:DNA, Recombinant / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D054794:Glycomics / Q000379:methods*; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant; D037102:Lectins; D011994:Recombinant Proteins