title: Multiple-locus variable-number tandem-repeat analysis is a suitable tool for differentiation of Mycoplasma hyopneumoniae strains without cultivation.
abstract: An assay based on multiple-locus variable-number tandem-repeat analysis allowed differentiating and studying diversity and persistence of Mycoplasma hyopneumoniae strains in pig herds without prior cultivation. The test had a discriminatory index of >0.99 and was applied reliably to porcine bronchoalveolar lavage fluid and tracheal swabs.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. katleen.vranckx@UGent.be
authors.forename: K
authors.initials: K
authors.lastname: Vranckx
authors.forename: D
authors.initials: D
authors.lastname: Maes
authors.forename: D
authors.initials: D
authors.lastname: Calus
authors.forename: I
authors.initials: I
authors.lastname: Villarreal
authors.forename: F
authors.initials: F
authors.lastname: Pasmans
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D045705:Mycoplasma hyopneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D045729:Pneumonia of Swine, Mycoplasmal / Q000175:diagnosis* / Q000382:microbiology; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014132:Trachea / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial