title: Noninvasive molecular diagnosis of human visceral leishmaniasis.
abstract: Previously developed methods for noninvasive PCR diagnosis of visceral leishmaniasis (VL) have significant limitations. Diagnosis of VL using PCR and buccal swabs was evaluated in 307 subjects, including 148 patients confirmed to have VL. This method is simple and well tolerated and has good potential for development, showing 83% sensitivity with 90.56% specificity in control groups.
authors.affiliation: Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. drshyamsundar@hotmail.com
authors.forename: Manisha
authors.initials: M
authors.lastname: Vaish
authors.forename: Sanjana
authors.initials: S
authors.lastname: Mehrotra
authors.forename: Jaya
authors.initials: J
authors.lastname: Chakravarty
authors.forename: Shyam
authors.initials: S
authors.lastname: Sundar
mesh_terms: D006801:Humans; D007893:Leishmania donovani / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D009061:Mouth Mucosa / Q000469:parasitology*; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*