title: Nanoscale spectroscopy and imaging of hemoglobin.
abstract: Sub diffraction limited infrared absorption imaging of hemoglobin was performed by coupling IR optics with an atomic force microscope. Comparisons between the AFM topography and IR absorption images of micron sized hemoglobin features are presented, along with nanoscale IR spectroscopic analysis of the metalloprotein.
authors.affiliation: Nanophotonics Research Group, School of Physics, University College Dublin, Belfield, Dublin, Ireland. Eamonn.Kennedy@ucdconnect.ie
authors.forename: Eamonn
authors.initials: E
authors.lastname: Kennedy
authors.forename: Fiona
authors.initials: F
authors.lastname: Yarrow
authors.forename: James H
authors.initials: JH
authors.lastname: Rice
mesh_terms: D000042:Absorption; D006454:Hemoglobins / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008667:Metalloproteins / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*
chemical_list: D006454:Hemoglobins; D008667:Metalloproteins