title: Identification of a unique library of complex, but ordered, arrays of repetitive elements in the human genome and implication of their potential involvement in pathobiology.
abstract: Approximately 2% of the human genome is reported to be occupied by genes. Various forms of repetitive elements (REs), both characterized and uncharacterized, are presumed to make up the vast majority of the rest of the genomes of human and other species. In conjunction with a comprehensive annotation of genes, information regarding components of genome biology, such as gene polymorphisms, non-coding RNAs, and certain REs, is found in human genome databases. However, the genome-wide profile of unique RE arrangements formed by different groups of REs has not been fully characterized yet. In this study, the entire human genome was subjected to an unbiased RE survey to establish a whole-genome profile of REs and their arrangements. Due to the limitation in query size within the bl2seq alignment program (National Center for Biotechnology Information [NCBI]) utilized for the RE survey, the entire NCBI reference human genome was fragmented into 6206 units of 0.5M nucleotides. A number of RE arrangements with varying complexities and patterns were identified throughout the genome. Each chromosome had unique profiles of RE arrangements and density, and high levels of RE density were measured near the centromere regions. Subsequently, 175 complex RE arrangements, which were selected throughout the genome, were subjected to a comparison analysis using five different human genome sequences. Interestingly, three of the five human genome databases shared the exactly same arrangement patterns and sequences for all 175 RE arrangement regions (a total of 12,765,625 nucleotides). The findings from this study demonstrate that a substantial fraction of REs in the human genome are clustered into various forms of ordered structures. Further investigations are needed to examine whether some of these ordered RE arrangements contribute to the human pathobiology as a functional genome unit.
authors.affiliation: Burn Research, Shriners Hospitals for Children Northern California and Department of Surgery, University of California-Davis, 2425 Stockton Blvd., Sacramento, CA 95817, USA.
authors.forename: Kang-Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Young-Kwan
authors.initials: YK
authors.lastname: Lee
authors.forename: Deug-Nam
authors.initials: DN
authors.lastname: Kwon
authors.forename: Sophia
authors.initials: S
authors.lastname: Chiu
authors.forename: Victoria
authors.initials: V
authors.lastname: Chew
authors.forename: Hyungchul
authors.initials: H
authors.lastname: Rah
authors.forename: Gregory
authors.initials: G
authors.lastname: Kujawski
authors.forename: Ramzi
authors.initials: R
authors.lastname: Melhem
authors.forename: Karen
authors.initials: K
authors.lastname: Hsu
authors.forename: Cecilia
authors.initials: C
authors.lastname: Chung
authors.forename: David G
authors.initials: DG
authors.lastname: Greenhalgh
authors.forename: Kiho
authors.initials: K
authors.lastname: Cho
mesh_terms: D002874:Chromosome Mapping; D015723:Gene Library; D020022:Genetic Predisposition to Disease; D015894:Genome, Human; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*