title: Highly enantioselective bioreduction of N-methyl-3-oxo-3-(thiophen-2-yl) propanamide for the production of (S)-duloxetine.
abstract: Whole cells of Rhodotorula glutinis reduced N-methyl-3-oxo-3-(thiophen-2-yl) propanamide at 30 g/l to (S)-N-methyl-3-hydroxy-3-(2-thienyl) propionamide, an intermediate in the production of (S)-duloxetine, a blockbuster antidepressant drug, in 48 h. The reaction had excellent enantioselectivity (single enantiomer, >99.5% enantiomeric excess [ee]) with a >95% conversion.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Chuan-Gen
authors.initials: CG
authors.lastname: Tang
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhang-Qin
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Zhong-Liu
authors.initials: ZL
authors.lastname: Wu
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D000068736:Duloxetine Hydrochloride; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012248:Rhodotorula / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D013876:Thiophenes; D000068736:Duloxetine Hydrochloride