title: A simple and rapid procedure for purification of haptoglobin from human plasma fraction IV.
abstract: Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P.R. China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Sun
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qingqiang
authors.initials: Q
authors.lastname: Meng
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.forename: Bei
authors.initials: B
authors.lastname: Fan
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ma
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
mesh_terms: D001688:Biological Products / Q000302:isolation & purification; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D006242:Haptoglobins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010949:Plasma / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D012709:Serum Albumin / Q000302:isolation & purification
chemical_list: D001688:Biological Products; D006242:Haptoglobins; D012709:Serum Albumin; D000431:Ethanol; D011092:Polyethylene Glycols