title: Assessment of factors affecting micropropagation and ex vitro acclimatization of Nyctanthes arbor-tristis L.
abstract: Rapid differentiation of multiple shoots was observed in 94% of nodal explants of one year old Nyctanthes arbor-tristis L. plants. Shoot bud induction and multiplication took place on Murashige and Skoog (MS) medium supplemented with two cytokinins, i.e. Benzyladenine (BA) or Kinetin (Kn) either alone or in combination with different auxins, indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) or α-naphthalene acetic acid (NAA). Between different media, pH levels and growth regulators tried, the optimum condition for maximum regenerative response was obtained on MS + Kn (2.5 μM) + N AA (0.5 μM) media at 5.8 pH, forming cultures with 23.26 ± 0.89 number of shoots and 6.36 ± 0.80 cm shoot length after 8 weeks of culture. Histological sections confirmed the formation of multiple buds from nodal explants. Rooting was achieved ex vitro by dipping the basal ends of microshoots in 200 μM IBA for 30 min followed by their transplantation in sterile soilrite. The plantlets with well-developed shoot and root system were successfully established in garden soil and grown outside in a greenhouse with a 80% survival rate.
authors.affiliation: Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202 002, India. anism1@rediffmail.com
authors.forename: Anushi Arjumend
authors.initials: AA
authors.lastname: Jahan
authors.forename: M
authors.initials: M
authors.lastname: Anis
authors.forename: I M
authors.initials: IM
authors.lastname: Aref
mesh_terms: D000064:Acclimatization / Q000187:drug effects / Q000502:physiology*; D000613:Aminobutyrates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D003583:Cytokinins / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin / Q000494:pharmacology; D029592:Oleaceae / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology*; D018520:Plant Shoots / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*
chemical_list: D000613:Aminobutyrates; D003583:Cytokinins; D007210:Indoleacetic Acids; C014328:2-amino-4-phenylbutyric acid; D007701:Kinetin