title: Spontaneously immortalized Schwann cells from adult Fischer rat as a valuable tool for exploring neuron-Schwann cell interactions.
abstract: We established spontaneously immortalized Schwann cell lines from long-term cultures of adult Fischer 344 rat dorsal root ganglia (DRG) and peripheral nerves. One of these cell lines, designated immortalized Fischer rat Schwann cells 1 (IFRS1), showed spindle-shaped morphology; immunoreactivity for S100, p75 neurotrophin receptor (p75(NTR) ), glial fibrillary acidic protein (GFAP), laminin, and vimentin; and mRNA expression of neurotrophic factors (NGF, GDNF, and CNTF), neurotrophin receptors (p75(NTR) , truncated TrkB, and TrkC), cell adhesion molecules (L1, NCAM, and N-cadherin), myelin proteins [P0, PMP22, and myelin-associated glycoprotein (MAG)], transcription factors (Krox20, Sox10, and Oct6), neuregulin-1 receptors (ErbB2 and ErbB3), and an orphan G protein-coupled receptor (Gpr126). Conditioned medium (CM) obtained from IFRS1 cells exhibited potent biological activity for the promotion of neuronal survival and neurite outgrowth of cultured adult rat DRG neurons. Furthermore, light and electron microscopic analyses revealed that IFRS1 cells were capable of myelinating neurites while in coculture with adult rat DRG neurons. These findings indicate that IFRS1 cells possess some biological properties of mature Schwann cells and that the coculture system with adult DRG neurons and IFRS1 cells can be a useful tool for the study of peripheral nerve degeneration and regeneration.
authors.affiliation: Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan. sango-kz@igakuken.or.jp
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sango
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Emiko
authors.initials: E
authors.lastname: Kawakami
authors.forename: Shizuka
authors.initials: S
authors.lastname: Takaku
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ajiki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Watabe
mesh_terms: D000818:Animals; D001391:Azo Compounds; D002450:Cell Communication / Q000502:physiology*; D002461:Cell Line, Transformed / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000166:cytology; D005786:Gene Expression Regulation / Q000502:physiology; D007763:Lac Operon / Q000235:genetics; D046529:Microscopy, Electron, Transmission / Q000379:methods; D004676:Myelin Basic Protein / Q000378:metabolism; D018993:Myelin P0 Protein / Q000378:metabolism; D009281:Naphthalenes; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010525:Peripheral Nerves / Q000166:cytology; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D012583:Schwann Cells / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism
chemical_list: D001391:Azo Compounds; D017077:Culture Media, Conditioned; D004676:Myelin Basic Protein; D018993:Myelin P0 Protein; D009281:Naphthalenes; C488416:Tubb3 protein, rat; D014404:Tubulin; D020932:Nerve Growth Factor; C016118:Sudan Black B