title: A Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl.
abstract: Chronic myelogenous leukemia (CML), a clonal myeloproliferative disorder in adults, and some pediatric and adult acute lymphoblastic leukemias (ALLs) are characterized by the presence of a Philadelphia chromosome, t(9;22)(q34;q11) (1). In this chromosomal translocation, exons from a major breakpoint cluster region (M-bcr), located on chromosome 22q11, are joined to the c-abl proto-oncogene, located on chromosome 9q34. When this chromosomal translocation occurs in a hematopoietic stem cell, the resulting BCR/abl fusion protein has increased tyrosine kinase activity and a transforming capacity that is critical to the pathogenesis of these leukemic disorders.
authors.affiliation: Division of Medical Genetics, Department of Genetics, Department of Medicine, University of California, San Diego, La Jolla, CA.
authors.forename: L M
authors.initials: LM
authors.lastname: Wasserman