title: Agricultural recovery of a formerly radioactive area: II. Systematic proteomic characterization of flax seed development in the remediated Chernobyl area.
abstract: Molecular characterization of crop plants grown in remediated, formerly radioactive, areas could establish a framework for future agricultural use of these areas. Recently, we have established a quantitative reference map for mature flax seed proteins (Linum usitatissimum L.) harvested from a remediated plot in Chernobyl town. Herein we describe results from our ongoing studies of this subject, and provide a proteomics-based characterization of developing flax seeds harvested from same field. A quantitative approach, based on 2-dimensional electrophoresis (2-DE) and tandem mass spectrometry, yielded expression profiles for 379 2-DE spots through seed development. Despite the paucity of genomic resources for flax, the identity for 102 proteins was reliably determined. These proteins were sorted into 11 metabolic functional classes. Proteins of unknown function comprise the largest group, and displayed a pattern of decreased abundance throughout seed development. Analysis of the composite expression profiles for metabolic protein classes revealed specific expression patterns during seed development. For example, there was an overall decrease in abundance of the glycolytic enzymes during seed development.
authors.affiliation: Department of Reproduction and Developmental Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
authors.forename: Katarína
authors.initials: K
authors.lastname: Klubicová
authors.forename: Maksym
authors.initials: M
authors.lastname: Danchenko
authors.forename: Ludovit
authors.initials: L
authors.lastname: Skultety
authors.forename: Valentyna V
authors.initials: VV
authors.lastname: Berezhna
authors.forename: Andrea
authors.initials: A
authors.lastname: Hricová
authors.forename: Namik M
authors.initials: NM
authors.lastname: Rashydov
authors.forename: Martin
authors.initials: M
authors.lastname: Hajduch
mesh_terms: D000383:Agriculture; D047852:Chernobyl Nuclear Accident; D019597:Flax / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D020869:Gene Expression Profiling; D040901:Proteomics; D012639:Seeds / Q000254:growth & development*