title: Use of gene-modified keratinocytes and fibroblasts to enhance regeneration in a full skin defect.
abstract: BACKGROUND
  With the development of cell-based gene transfer techniques, genetically modified human keratinocytes (Kc) and fibroblasts (Fb) have been proven to be a better choice in wound repair.
  
  
  METHODS
  This study was designed to construct in one step a gene-modified artificial skin by a genetically engineered Kc expressing PDGF-BB and Fb expressing VEGF(165) and bFGF. The wound healing effect in a full-thickness wound model was then observed. Unmodified artificial skin served as control. On the post-operative days 7, 14, and 21, residual wound area was calculated and skin wound tissues were subjected to biopsy for further investigation.
  
  
  RESULTS
  Compared with unmodified artificial skin, gene-modified artificial skin resulted in a reduced wound contraction and a well-organized human epidermis and better formed dermis.
  
  
  CONCLUSIONS
  The results suggest that our two-layer, gene-modified artificial skin improved both vascularization and epidermalization for skin regeneration. This technique could bring about a new approach in the treatment of burns and chronic wounds.
authors.affiliation: Department for Plastic Surgery and Hand Surgery, Klinikum rechts der Isar, Technische Universität München, Ismaninger Str. 22, 81675, Munich, Germany. lohmeyer@lrz.tum.de
authors.forename: Jörn Andreas
authors.initials: JA
authors.lastname: Lohmeyer
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Stefan
authors.initials: S
authors.lastname: Krüger
authors.forename: Werner
authors.initials: W
authors.lastname: Lindenmaier
authors.forename: Frank
authors.initials: F
authors.lastname: Siemers
authors.forename: Hans-Günther
authors.initials: HG
authors.lastname: Machens
mesh_terms: D000818:Animals; D000077214:Becaplermin; D002809:Chondroitin Sulfates / Q000235:genetics / Q000627:therapeutic use*; D003094:Collagen / Q000235:genetics / Q000627:therapeutic use*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D005347:Fibroblasts / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D015603:Keratinocytes / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis; D017768:Skin, Artificial; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D014945:Wound Healing / Q000235:genetics*
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C114835:integra artificial skin; D016222:Fibroblast Growth Factor 2; D000077214:Becaplermin; D002809:Chondroitin Sulfates; D003094:Collagen