title: Determination of salidroside and tyrosol in Rhodiola by capillary electrophoresis with graphene/poly(urea-formaldehyde) composite modified electrode.
abstract: This report describes the fabrication and application of a novel graphene/poly(urea-formaldehyde) composite modified electrode as a sensitive amperometric detector of CE. The composite electrode was fabricated on the basis of the in situ polycondensation of a mixture of graphenes and urea-formaldehyde prepolymers on the surface of a platinum disc electrode. It was coupled with CE for the separation and detection of salidroside and tyrosol in Rhodiola, a traditional Chinese medicine, to demonstrate its feasibility and performance. Salidroside and tyrosol have been well separated within 6 min in a 40 cm long capillary at a separation voltage of 12 kV using a 50 mM borate buffer (pH 9.8). The prepared graphene-based CE detector offered significantly lower detection potential, yielded enhanced signal-to-noise characteristics, and exhibited high resistance to surface fouling and enhanced stability. It showed long-term stability and reproducibility with relative standard deviations of less than 5% for the peak current (n = 15).
authors.affiliation: School of Pharmacy, Fudan University, Shanghai, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Luyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005557:Formaldehyde / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D006108:Graphite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D029771:Rhodiola / Q000737:chemistry*; D014508:Urea / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; D011108:Polymers; C011867:4-hydroxyphenylethanol; D005557:Formaldehyde; C028278:urea formaldehyde foam; D006108:Graphite; D014508:Urea; C009172:rhodioloside; D010626:Phenylethyl Alcohol