title: HPLC analysis of kaempherol and quercetin derivatives isolated by different extraction techniques from plant matrix.
abstract: Soxhlet extraction, ultrasound extraction, and accelerated solvent extraction (ASE), followed by RP-HPLC with a photodiode array detector was used for the determination of flavonoids in fruits of Peucedanum alsaticum. Three compounds were identified: a kaempherol derivative (astragalin), and two quercetin derivatives (quercitrin and hiperoside). The highest extraction yields of the selected compounds were obtained by use of exhaustive ASE.
authors.affiliation: Medical University of Lublin, Department of Pharmacognosy with Medicinal Plant Unit, 1 Chodzki St, 20-093 Lublin, Poland. kskalicka@pharmacognosy.org
authors.forename: Krystyna
authors.initials: K
authors.lastname: Skalicka-Woźniak
authors.forename: Janusz
authors.initials: J
authors.lastname: Szypowski
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Głowniak
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry; D044949:Kaempferols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D005419:Flavonoids; D044949:Kaempferols; D010936:Plant Extracts; C012526:quercitrin; D011794:Quercetin; C001579:astragalin