title: Study of a novel cell lysis method with titanium dioxide for Lab-on-a-Chip devices.
abstract: In this paper, a novel method is proposed and demonstrated to be able to lyse gram-negative (E. coli) bacteria cells for Lab-on-a-Chip applications. The proposed method incorporates using titanium dioxide particles as photocatalysts and a miniaturized UV LED array as an excitation light source to perform cell lysis on microchips. The experimental result demonstrates the feasibility of the proposed prototype device. The working device suggests an inexpensive, easy to be fabricated and effective way for microchip cell lysis. The miniaturized UV LED array and the microchip with a reaction chamber can be easily integrated with other functional components to form a customized whole Lab-on-a-Chip system.
authors.affiliation: Department of Systems Design Engineering, University of Waterloo, Waterloo, Canada.
authors.forename: Weijie
authors.initials: W
authors.lastname: Wan
authors.forename: John T W
authors.initials: JT
authors.lastname: Yeow
mesh_terms: D003584:Cytological Techniques / Q000295:instrumentation*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D056656:Lab-On-A-Chip Devices; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: C009495:titanium dioxide; D014025:Titanium