title: Development and characterization of a disposable plastic microarray printhead.
abstract: During the last decade microarrays have become a powerful analytical tool. Commonly microarrays are produced in a non-contact manner using silicone printheads. However, silicone printheads are expensive and not able to be used as a disposable. Here, we show the development and functional characterization of 8-channel plastic microarray printheads that overcome both disadvantages of their conventional silicone counterparts. A combination of injection-molding and laser processing allows us to produce a high quantity of cheap, customizable and disposable microarray printheads. The use of plastics (e.g., polystyrene) minimizes the need for surface modifications required previously for proper printing results. Time-consuming regeneration processes, cleaning procedures and contaminations caused by residual samples are avoided. The utilization of plastic printheads for viscous liquids, such as cell suspensions or whole blood, is possible. Furthermore, functional parts within the plastic printhead (e.g., particle filters) can be included. Our printhead is compatible with commercially available TopSpot devices but provides additional economic and technical benefits as compared to conventional TopSpot printheads, while fulfilling all requirements demanded on the latter. All in all, this work describes how the field of traditional microarray spotting can be extended significantly by low cost plastic printheads.
authors.affiliation: Fraunhofer Institute for Biomedical Engineering, Am Mühlenberg 13, Potsdam, Germany. Matthias.Griessner@ibmt.fraunhofer.de
authors.forename: Matthias
authors.initials: M
authors.lastname: Griessner
authors.forename: Dave
authors.initials: D
authors.lastname: Hartig
authors.forename: Alexander
authors.initials: A
authors.lastname: Christmann
authors.forename: Carsten
authors.initials: C
authors.lastname: Pohl
authors.forename: Michaela
authors.initials: M
authors.lastname: Schellhase
authors.forename: Eva
authors.initials: E
authors.lastname: Ehrentreich-Förster
mesh_terms: D002470:Cell Survival; D004209:Disposable Equipment; D004867:Equipment Design; D005374:Filtration; D006367:HeLa Cells; D006801:Humans; D007267:Injections; D007834:Lasers; D046228:Microarray Analysis / Q000295:instrumentation*; D010969:Plastics; D011327:Printing / Q000295:instrumentation*; D046888:Tissue Array Analysis; D014783:Viscosity
chemical_list: D010969:Plastics