title: Diagnosis and molecular characterization of rabies virus from a buffalo in China: a case report.
abstract: BACKGROUND
  Rabies virus (RABV) can infect many different species of warm-blooded animals. Glycoprotein G plays a key role in viral pathogenicity and neurotropism, and includes antigenic domains that are responsible for membrane fusion and host cell receptor recognition.
  
  
  METHODS
  A case of buffalo rabies in China was diagnosed by direct fluorescent antibody test, G gene reverse-transcriptase polymerase chain reaction, and RABV mouse inoculation test. Molecular characterization of the RABV was performed using DNA sequencing, phylogenetic analysis and amino acid sequence comparison based on the G gene from different species of animals.
  
  
  CONCLUSIONS
  The results confirmed that the buffalo with suspected rabies was infected by RABV, which was genetically closely related to HNC (FJ602451) that was isolated from cattle in China in 2007. Comparison of the G gene among different species of animal showed that there were almost no amino acid changes among RABVs isolated from the same species of animals that distributed in a near region. However, there were many changes among RABVs that were isolated from different species of animal, or the same species from different geographic regions. This is believed to be the first case report of buffalo rabies in China, and the results may provide further information to understand the mechanism by which RABV breaks through the species barrier.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China.
authors.forename: Ke-Shan
authors.initials: KS
authors.lastname: Zhang
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Guo
authors.forename: Zhuo-Fei
authors.initials: ZF
authors.lastname: Xu
authors.forename: Min
authors.initials: M
authors.lastname: Xiang
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Huan-Chun
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000820:Animal Diseases / Q000175:diagnosis* / Q000821:virology; D000818:Animals; D002020:Buffaloes / Q000821:virology*; D002417:Cattle; D002681:China; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins; C472447:rabies virus glycoprotein (253-275)