title: Localization of the orexin system in the gastrointestinal tract of fallow deer.
abstract: The aim of the present study was to investigate by immunohistochemistry the presence and distribution of the orexin system in the stomach and gut of fallow deer. Abundant orexin A-positive cells were localized in the middle and basal portions of the mucosal glands of the cardial and fundic regions of the stomach. In the same gastric areas, orexin B-positive cells were also found, mainly localized in the basal portion of glands. In the intestinal tract, orexin-containing cells were occasionally found in the duodenal epithelium and in the rectal intestinal glands. Immunoreactivity for orexin receptors, type 1 and 2 (OX1R and OX2R), was not detected in the same stomach regions. OX1R-immunopositivity was observed in the enteric neuron ganglia localized in the submucosal and muscular intestinal layers, while OX2R-immunopositivity was found close in contact with the cytoplasmic membrane of epithelial cells in the small intestine.
authors.affiliation: Sezione di Anatomia Veterinaria, Dipartimento di Scienze Biopatologiche Veterinarie ed Igiene delle Produzioni Animali ed Alimentari, Perugia, Italy. cecilia.dallaglio@unipg.it
authors.forename: Cecilia
authors.initials: C
authors.lastname: Dall'Aglio
authors.forename: Luisa
authors.initials: L
authors.lastname: Pascucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Mercati
authors.forename: Cristiano
authors.initials: C
authors.lastname: Boiti
authors.forename: Piero
authors.initials: P
authors.lastname: Ceccarelli
mesh_terms: D000018:Abomasum; D000818:Animals; D003670:Deer / Q000033:anatomy & histology* / Q000378:metabolism; D004386:Duodenum / Q000166:cytology / Q000294:innervation / Q000378:metabolism*; D017615:Enteric Nervous System / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005725:Ganglia, Autonomic / Q000166:cytology / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D041741:Lower Gastrointestinal Tract / Q000166:cytology / Q000294:innervation / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D064446:Orexin Receptors; D000068797:Orexins; D009928:Organ Specificity; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D018013:Receptors, Neuropeptide / Q000378:metabolism*; D013270:Stomach / Q000166:cytology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D064446:Orexin Receptors; D000068797:Orexins; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide