title: RNA slot blotting.
abstract: Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs expressed in the same tissues being examined-for example, RNAs derived from a hybrid gene with a viral or prokaryotic reporter element or a gene from another species with sufficient sequence divergence to allow the transgene RNAs to be distinguished from the host RNAs.
authors.affiliation: Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.
authors.forename: D
authors.initials: D
authors.lastname: Murphy
mesh_terms: D015336:Molecular Probe Techniques; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012313:RNA