title: Identification of the CD34 enumeration on the day before stem cell harvest that best predicts poor mobilization.
abstract: BACKGROUND
  We questioned whether CD34 enumeration in peripheral blood on the day before planned collection would identify a patient population that could benefit from an augmented collection strategy during that mobilization attempt.
  
  
  METHODS
  A retrospective review of all adult patients who underwent a first mobilization attempt between January and December 2008 for autologous use was undertaken. Peripheral blood CD34 quantitation on the day before planned collection (Day -1) and day of planned collection (Day 0) was correlated with likelihood of a successful collection.
  
  
  RESULTS
  Of 41 patients (15 with multiple myeloma, 20 with lymphoma, and six with other malignancies) who underwent mobilization 24 patients (58%) were harvested in 1 day (good mobilizers) with the remaining 17 patients (42%) either requiring more than 1 day to collect or were not collected (poor mobilizers [PMs]). A peripheral blood CD34+ count below 10 × 10(6) /L on Day -1 was optimal in identifying PMs (adjusted odds ratio of 7 [1.4-35]). Increasing the CD34 cutoff from 10 × 10(6) to 15 × 10(6) /L decreased the prediction of poor mobilization (positive likelihood ratio dropped from 3.3 to 2.2).
  
  
  CONCLUSIONS
  Peripheral blood CD34 content of less than or equal to 10 × 10(6) CD34+ cells/L on the day before collection is predictive of poor mobilization whereas higher peripheral blood CD34 counts on Day -1 have a high likelihood of successful 1-day collection.
authors.affiliation: Department of Internal Medicine and Pediatrics and Child Health, University of Manitoba and CancerCare Manitoba, Winnipeg, Manitoba, Canada. david.szwajcer@cancercare.mb.ca
authors.forename: David
authors.initials: D
authors.lastname: Szwajcer
authors.forename: Alana
authors.initials: A
authors.lastname: Jennings-Coutts
authors.forename: Angeline
authors.initials: A
authors.lastname: Giftakis
authors.forename: Donna A
authors.initials: DA
authors.lastname: Wall
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D001772:Blood Cell Count; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies
chemical_list: D018952:Antigens, CD34