title: Overexpression of survivin and cyclin D1 in CHO cells confers apoptosis resistance and enhances growth in serum-free suspension culture.
abstract: To optimize Chinese hamster ovary (CHO) cell culture to recombinant protein therapeutic production, we stably overexpressed survivin and cyclin D1 in three CHO DG44-derived cell lines. The modifications conferred increases of 56-94% in S-phase fractions and decreases of 33-43% in early-stage apoptosis fractions. Clone 6.3, which expressed the highest levels of survivin and cyclin D1, reached significantly greater cell densities in suspension (2.7 × 10(6) cells/ml) following serum deprivation. Nude mice inoculated with the modified cells showed no tumorigenesis suggesting that the CHO DG44-derived cell lines are viable candidates for biopharmaceutical production.
authors.affiliation: State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dongdajie Street, Fengtai District, Beijing, People's Republic of China.
authors.forename: Xinghui
authors.initials: X
authors.lastname: Zhao
authors.forename: Junwei
authors.initials: J
authors.lastname: Guo
authors.forename: Yingqun
authors.initials: Y
authors.lastname: Yu
authors.forename: Shaoqiong
authors.initials: S
authors.lastname: Yi
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D016466:CHO Cells / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D019938:Cyclin D1 / Q000096:biosynthesis*; D015870:Gene Expression; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D016895:Culture Media, Serum-Free; D051034:Inhibitor of Apoptosis Proteins; D011994:Recombinant Proteins; D019938:Cyclin D1