title: Proteome and membrane fatty acid analyses on Oligotropha carboxidovorans OM5 grown under chemolithoautotrophic and heterotrophic conditions.
abstract: Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium able to utilize CO and H(2) to derive energy for fixation of CO(2). Thus, it is capable of growth using syngas, which is a mixture of varying amounts of CO and H(2) generated by organic waste gasification. O. carboxidovorans is capable also of heterotrophic growth in standard bacteriologic media. Here we characterize how the O. carboxidovorans proteome adapts to different lifestyles of chemolithoautotrophy and heterotrophy. Fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown with acetate or with syngas showed that the bacterium changes membrane fatty acid composition. Quantitative shotgun proteomic analysis of O. carboxidovorans grown in the presence of acetate and syngas showed production of proteins encoded on the megaplasmid for assimilating CO and H(2) as well as proteins encoded on the chromosome that might have contributed to fatty acid and acetate metabolism. We found that adaptation to chemolithoautotrophic growth involved adaptations in cell envelope, oxidative homeostasis, and metabolic pathways such as glyoxylate shunt and amino acid/cofactor biosynthetic enzymes.
authors.affiliation: College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, United States of America.
authors.forename: Debarati
authors.initials: D
authors.lastname: Paul
authors.forename: Ranjit
authors.initials: R
authors.lastname: Kumar
authors.forename: Bindu
authors.initials: B
authors.lastname: Nanduri
authors.forename: Todd
authors.initials: T
authors.lastname: French
authors.forename: Ken
authors.initials: K
authors.lastname: Pendarvis
authors.forename: Ashli
authors.initials: A
authors.lastname: Brown
authors.forename: Mark L
authors.initials: ML
authors.lastname: Lawrence
authors.forename: Shane C
authors.initials: SC
authors.lastname: Burgess
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D041841:Bradyrhizobiaceae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D052837:Chemoautotrophic Growth / Q000502:physiology*; D016000:Cluster Analysis; D005227:Fatty Acids / Q000032:analysis*; D053263:Gene Regulatory Networks / Q000502:physiology; D005798:Genes, Bacterial; D006038:Glyoxylates / Q000378:metabolism; D052836:Heterotrophic Processes / Q000502:physiology*; D008563:Membrane Lipids / Q000032:analysis; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010084:Oxidation-Reduction; D020543:Proteome / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D006038:Glyoxylates; D008563:Membrane Lipids; D020543:Proteome; C031150:glyoxylic acid