title: Development of rabbit meniscus acellular matrix.
abstract: OBJECTIVE
  To prepare a rabbit meniscus acellular matrix scaffold and explore the histomorphological and biomechanical properties of the scaffold.
  
  
  METHODS
  Rabbit meniscuses were collected and acellularized using a modified eight-step detergent process with hydrogen peroxide, distilled water, Triton X-100, and sodium deoxycholate. Its color and texture were observed. Histomorphological assessment was performed using routine hematoxylin-eosin stain, toluidine blue stain, Saffron stain, Hoechst-33258 stain, and immunohistochemical staining of collagen I. The ultrastructure of the specimens was observed with inverted phase contrast microscopy. Transient recovery rate of deformation, maximal recovery rate of deformation, and maximal compressive strength were tested to determine the biomechanical properties of the scaffold.
  
  
  RESULTS
  The processed meniscus was milk-white in color with loose structure. It histologically appeared cell-free, stained positively for collagen I, and had abundant micropores according to phase-contrast microscopy. The transient recovery rate of deformation was (76.65∓4.61)%, the maximal recovery rate of deformation was 100%, and the maximal compressive strength was (4.51∓0.69) N when the specimens were compressed 40%.
  
  
  CONCLUSIONS
  The rabbit meniscus acellular matrix scaffold, with numerous micropores, is easy to be recovered from deformation and suitable for the adhesiveness and growth of breeding cells. This scaffold can be used as an ideal implant for future tissue engineering of the meniscus.
authors.affiliation: Department of Orthopeadics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yu
authors.forename: Liu
authors.initials: L
authors.lastname: Yu Jie
authors.forename: Huang
authors.initials: H
authors.lastname: Jing Xiang
authors.forename: Diao
authors.initials: D
authors.lastname: Bin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis; D001696:Biomechanical Phenomena; D008422:Materials Testing; D008592:Menisci, Tibial / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials