title: Effects of a 1.5 T time-varying magnetic field on cell volume regulation of bovine adrenal chromaffin cells in hyposmotic media.
abstract: Effects of a time-varying magnetic field on cell volume regulation by hyposmotic stress in cultured bovine adrenal chromaffin cells were examined. Through regulatory volume decrease (RVD), cell volume of chromaffin cells that were incubated in a hypotonic medium initially increased, reached a peak and finally recovered to the initial value. Two hour exposure to a magnetic field and addition of cytochalasin D increased peak value and delayed return to initial value. Intracellular F-actin contents initially decreased but returned to normal levels after 10 sec. Two hour exposure to the magnetic field and addition of cytochalasin D continuously reduced the F-actin content. Results suggest that exposure to the magnetic field stimulated disruption of the actin cytoskeleton and that the disruption delayed the recovery to the volume prior to osmotic stress.
authors.affiliation: Division of Pharmacy, Tokushima University Hospital, Tokushima, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Nishisako
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Ikehara
authors.forename: Tairoh
authors.initials: T
authors.lastname: Shiraishi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Minami
authors.forename: Hiromi Ichinose
authors.initials: HI
authors.lastname: Sasaki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Houchi
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawazoe
authors.forename: Kazuo
authors.initials: K
authors.lastname: Minakuchi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kitamura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shono
authors.forename: Kazuo
authors.initials: K
authors.lastname: Yoshizaki
authors.forename: Yohsuke
authors.initials: Y
authors.lastname: Kinouchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyamoto
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D015638:Cytochalasin D / Q000494:pharmacology; D007038:Hypotonic Solutions; D008280:Magnetics; D009997:Osmotic Pressure; D013997:Time Factors
chemical_list: D000199:Actins; D007038:Hypotonic Solutions; D015638:Cytochalasin D; D002118:Calcium