title: Heritability of genetic variants of resistin gene in patients with coronary artery disease: a family-based study.
abstract: OBJECTIVE
  The resistin gene (RETN) -420C>G and +299G>A polymorphism was investigated in a case-control study from forty complex Pakistani families with coronary artery disease (CAD) history. Heritability of the susceptible/variant alleles was investigated from parent-offspring trios in these families.
  
  
  METHODS
  Resistin levels were determined from 239 individuals by enzyme-linked immunosorbent assay. Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism.
  
  
  RESULTS
  Elevated resistin levels were observed from CAD cases vs. controls (p<0.0001). The RETN -420C>G and +299G>A polymorphism was more prevalent in cases vs. controls (p<0.0001). The transmission disequilibrium test revealed a significant association of the -420 and +299 polymorphism with CAD (χ(2)=34.4, p<0.0001 and χ(2)=31.6, p<0.0001, respectively).
  
  
  CONCLUSIONS
  Elevated resistin, and the RETN -420C>G and +299G>A polymorphism may contribute to familial CAD. The -420 and +299 variant alleles are transmitted more frequently from parent to affected offspring. This is the first report on the association of the RETN +299G>A polymorphism with CAD.
authors.affiliation: Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Sabir
authors.initials: S
authors.lastname: Hussain
authors.forename: Shakeela
authors.initials: S
authors.lastname: Bibi
authors.forename: Qamar
authors.initials: Q
authors.lastname: Javed
mesh_terms: D000328:Adult; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D052243:Resistin / Q000235:genetics*
chemical_list: D052243:Resistin