title: Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system.
abstract: Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.
authors.affiliation: Eunice Kennedy Shriver National Institute of Child Healthand Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Ting
authors.forename: Stephanie
authors.initials: S
authors.lastname: Gu
authors.forename: Sudha
authors.initials: S
authors.lastname: Guttikonda
authors.forename: Tzu-Yang
authors.initials: TY
authors.lastname: Lin
authors.forename: Benjamin H
authors.initials: BH
authors.lastname: White
authors.forename: Chi-Hon
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D011401:Promoter Regions, Genetic / Q000235:genetics; D012697:Serine Endopeptidases / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics*; D000099160:LexA Repressor Protein
chemical_list: D001426:Bacterial Proteins; D029721:Drosophila Proteins; D012697:Serine Endopeptidases; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C557617:GAL4 protein, Drosophila