title: Whole-cell biosensors for detection of heavy metal ions in environmental samples based on metallothionein promoters from Tetrahymena thermophila.
abstract: Heavy metals are among the most serious pollutants, and thus there is a need to develop sensitive and rapid biomonitoring methods for heavy metals in the environment. Critical parameters such as bioavailability, toxicity and genotoxicity cannot be tested using chemical analysis, but only can be assayed using living cells. A whole-cell biosensor uses the whole cell as a single reporter incorporating both bioreceptor and transducer elements. In the present paper, we report results with two gene constructs using the Tetrahymena thermophila MTT1 and MTT5 metallothionein promoters linked with the eukaryotic luciferase gene as a reporter. This is the first report of a ciliated protozoan used as a heavy metal whole-cell biosensor. T. thermophila transformed strains were created as heavy metal whole-cell biosensors, and turn on bioassays were designed to detect, in about 2 h, the bioavailable heavy metals in polluted soil or aquatic samples. Validation of these whole-cell biosensors was carried out using both artificial and natural samples, including methods for detecting false positives and negatives. Comparison with other published cell biosensors indicates that the Tetrahymena metallothionein promoter-based biosensors appear to be the most sensitive eukaryotic metal biosensors and compare favourably with some prokaryotic biosensors as well.
authors.affiliation: Departamento de Microbiología-III, Facultad de Biología, C/. José Antonio Novais 2, Universidad Complutense, Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Amaro
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Turkewitz
authors.forename: Ana
authors.initials: A
authors.lastname: Martín-González
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Gutiérrez
mesh_terms: D019677:Artificial Gene Fusion; D015374:Biosensing Techniques / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D007477:Ions / Q000032:analysis; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D008668:Metallothionein / Q000235:genetics*; D019216:Metals, Heavy / Q000032:analysis*; D011401:Promoter Regions, Genetic; D012680:Sensitivity and Specificity; D016808:Tetrahymena thermophila / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013997:Time Factors
chemical_list: D004785:Environmental Pollutants; D007477:Ions; D019216:Metals, Heavy; D008668:Metallothionein; D008156:Luciferases