title: Recombination centres and the orchestration of V(D)J recombination.
abstract: The initiation of V(D)J recombination by the recombination activating gene 1 (RAG1) and RAG2 proteins is carefully orchestrated to ensure that antigen receptor gene assembly occurs in the appropriate cell lineage and in the proper developmental order. Here we review recent advances in our understanding of how DNA binding and cleavage by the RAG proteins are regulated by the chromatin structure and architecture of antigen receptor genes. These advances suggest novel mechanisms for both the targeting and the mistargeting of V(D)J recombination, and have implications for how these events contribute to genome instability and lymphoid malignancy.
authors.affiliation: Department of Immunobiology and Howard Hughes Medical Institute, Yale University School of Medicine, 300 Cedar Street, Box 208011, New Haven, Connecticut 06520-8011, USA. david.schatz@yale.edu
authors.forename: David G
authors.initials: DG
authors.lastname: Schatz
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015329:Gene Rearrangement, T-Lymphocyte; D016654:Genes, RAG-1 / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D011946:Receptors, Antigen / Q000235:genetics; D011995:Recombination, Genetic; D050441:VDJ Exons / Q000235:genetics*; D045567:VDJ Recombinases / Q000235:genetics
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D011946:Receptors, Antigen; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein; D045567:VDJ Recombinases