title: Scope and limitation of side-chain assisted ligation.
abstract: Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel.
authors.forename: Liat
authors.initials: L
authors.lastname: Spasser
authors.forename: K S
authors.initials: KS
authors.lastname: Ajish Kumar
authors.forename: Ashraf
authors.initials: A
authors.lastname: Brik
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D013438:Sulfhydryl Compounds