title: Improved preservation and microcirculation with POLYSOL after partial liver transplantation in rats.
abstract: BACKGROUND
  Due to the severe shortage of deceased donors, demand for living-donor liver transplantation (LDLT) has increased worldwide. Here, we compared POLYSOL, a recently developed low-viscosity preservation solution, and histidine-tryptophan-ketoglutarate (HTK) for cold storage of partial liver graft in this study.
  
  
  METHODS
  Partial liver transplantations with 30% of the native liver were performed in Lewis rats. The graft livers were flushed with either HTK or POLYSOL (n = 25, respectively) and stored in the respective solution for 3 h at 5°C. Graft function was evaluated regarding ischemia-reperfusion injury and regeneration at 1, 3, 24, and 168 h after reperfusion.
  
  
  RESULTS
  POLYSOL preservation resulted in improvement of portal venous flow (HTK versus POLYSOL; mean ± SEM: 16.8 ± 2.2 versus 21.6 ± 2.1 mL/min; P = 0.005), microcirculation (383 ± 63 versus 532 ± 64 Flux; P = 0.045), ALT (310.2 ± 56.1 versus 181.8 ± 17.0 IU/L; P = 0.0262), LDH (4052.4 ± 764.4 versus 2494.1 ± 410.0 IU/L; P = 0.0215), total bilirubin (21.6 ± 14.2 versus 4.0 ± 0.6 IU/L; P = 0.0236), malondialdehyde (100.0 ± 4.3 versus 69.2 ± 4.0 nmol/mL; P = 0.0015), as well histologic findings at 24 h. Liver regeneration was improved in POLYSOL with regards to liver weight (4.0 ± 0.2 versus 4.3 ± 0.3 g; P = 0.038) and Ki-67 labeling index (9.67 ± 2.17 versus 1.10 ± 0.14%; P < 0.0001) at 24 h with higher up-regulation of portal VEGF (31.55 ± 5.78 versus 91.94 ± 9.27 pg/mL; P = 0.0052).
  
  
  CONCLUSIONS
  This study showed that POLYSOL improves microcirculation and thus improves the preservation quality of partial liver transplantation.
authors.affiliation: Institute for Laboratory Animal Science and Experimental Surgery, RWTH-Aachen University, Aachen, Germany.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yagi
authors.forename: Benedict Marie
authors.initials: BM
authors.lastname: Doorschodt
authors.forename: Mamdouh
authors.initials: M
authors.lastname: Afify
authors.forename: Uwe
authors.initials: U
authors.lastname: Klinge
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.forename: Shinji
authors.initials: S
authors.lastname: Uemoto
authors.forename: Rene H
authors.initials: RH
authors.lastname: Tolba
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D003080:Cold Temperature; D005947:Glucose / Q000627:therapeutic use; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000502:physiology*; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000627:therapeutic use; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology / Q000627:therapeutic use*; D011189:Potassium Chloride / Q000627:therapeutic use; D011343:Procaine / Q000627:therapeutic use; D051381:Rats; D011917:Rats, Inbred Lew; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; C504758:polysol solution; D008353:Mannitol; D008315:Malondialdehyde; D011343:Procaine; D011189:Potassium Chloride; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase; D005947:Glucose; D001663:Bilirubin