title: Polyelectrolyte microcapsules with entrapped multicellular tumor spheroids as a novel tool to study the effects of photodynamic therapy.
abstract: In the current study, semi-permeable alginate-oligochitosan microcapsules for multicellular tumor spheroids (MTS) generation were elaborated and tested, to estimate a response of the microencapsulated MTS (MMTS) to photodynamic therapy (PDT). The microcapsules (mean diameter 600 μm) with entrapped human breast adenocarcinoma MCF-7 cells were obtained using an electrostatic bead generator, and MMTS were generated by in vitro long-term cell cultivation. The formed MMTS were incubated in Chlorin e6 photosensitizer solution and then irradiated using 650-nm laser light. The cell viability was measured by MTT-assay in 24 h after irradiation, and histological analysis was performed. The proposed MTS-based model was found to be more resistant to the PDT than the two-dimensional monolayer cell culture model. Thus, MMTS could be considered as a promising three-dimesional in vitro model to estimate the doses of drugs or parameters for PDT in vitro before carrying out preclinical tests.
authors.affiliation: Polymers for Biology Laboratory, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, 16/10, Moscow 117997, Russia. dariaz.z@gmail.com
authors.forename: Daria S
authors.initials: DS
authors.lastname: Zaytseva-Zotova
authors.forename: Olga O
authors.initials: OO
authors.lastname: Udartseva
authors.forename: Elena R
authors.initials: ER
authors.lastname: Andreeva
authors.forename: Artur
authors.initials: A
authors.lastname: Bartkowiak
authors.forename: Lina N
authors.initials: LN
authors.lastname: Bezdetnaya
authors.forename: François
authors.initials: F
authors.lastname: Guillemin
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Goergen
authors.forename: Elena A
authors.initials: EA
authors.lastname: Markvicheva
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy*; D002214:Capsules / Q000737:chemistry / Q000627:therapeutic use*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism; D002735:Chlorophyllides; D005260:Female; D006801:Humans; D008422:Materials Testing; D008954:Models, Biological; D010316:Particle Size; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D011166:Porphyrins / Q000627:therapeutic use; D018874:Spheroids, Cellular; D055672:Static Electricity
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; C062985:phytochlorin; D048271:Chitosan