title: Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [(32)P]-Oligomers.
abstract: Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1-3). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides (antigens) are expressed as a β-galactosidase fusion protein in E. coli. Because the lacZ gene has a strong promoter, the fusion proteins comprise from 0.1-4% of the total E. coli proteins (1-3). The expressed fusion antigens are transferred onto nitrocellulose filters and then probed with antibodies to identify the targets containing the specific, cognate epitope.
authors.affiliation: Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Hur
authors.forename: D
authors.initials: D
authors.lastname: Lai