title: Imitation of biomembranes on the basis of cholic acid and endogenic thermostable protein complex in biopartitioning micellar chromatography.
abstract: The structure of biomembranes was imitated by introducing nonionic surfactant polyoxyethylene (23) dodecylether, cholic acid and endogenic thermostable protein complex (14-65 kDa) into the mobile phase. The influence of concentration of these additives on the retention of the model compounds was studied. The competing interaction of cholic acid and endogenic thermostable protein complex in the lipid bilayer model was revealed on the basis of chromatographic data. The values of efficiency of the chromatographic column regarding solutes were increased by addition of endogenic thermostable protein complex to the mobile phase containing Brij-35 and cholic acid.
authors.affiliation: Faculty of Exact and Natural Sciences, Ivane Javakhishvili Tbilisi State University, 0128, Tbilisi, Georgia. marina_rukhadze@yahoo.com
authors.forename: Marina
authors.initials: M
authors.lastname: Rukhadze
authors.forename: Diana
authors.initials: D
authors.lastname: Dzidziguri
authors.forename: Nana
authors.initials: N
authors.lastname: Giorgobiani
authors.forename: Salome
authors.initials: S
authors.lastname: Kerkenjia
mesh_terms: D000818:Animals; D019826:Cholic Acid / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008823:Micelles; D008954:Models, Biological; D008956:Models, Chemical; D009525:Niacin / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000737:chemistry; D051381:Rats; D012439:Saccharin / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D008823:Micelles; D013501:Surface-Active Agents; D009525:Niacin; D011092:Polyethylene Glycols; D012439:Saccharin; D019826:Cholic Acid; C515901:Brij 35