title: Gonadotropin purification from horse serum applying magnetic beads.
abstract: The glycoprotein hormone equine chorionic gonadotropin (eCG) is a commercial product used in animal breeding as well as in veterinary medicine. The current state of the art for the purification of eCG from serum is pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Two simplified processes, including the use of magnetic microsorbents for the purification of eCG have been developed. The processes reduce or even omit the use of organic solvents and the required solid-liquid separation steps, thus making them potential candidates for a first commercial application of magnetic beads in bioprocessing.
authors.affiliation: Karlsruhe Institute of Technology, Institute of Functional Interfaces, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Müller
authors.forename: Andrea
authors.initials: A
authors.lastname: Preusser-Kunze
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wagner
authors.forename: Matthias
authors.initials: M
authors.lastname: Franzreb
mesh_terms: D000042:Absorption; D000818:Animals; D006063:Chorionic Gonadotropin / Q000097:blood* / Q000302:isolation & purification*; D006736:Horses; D008280:Magnetics
chemical_list: D006063:Chorionic Gonadotropin