title: Methodology and technology for stabilization of specific states of signal transduction proteins.
abstract: The ability to adequately measure the phosphorylation state of a protein has major biological as well as clinical relevance. Due to its variable nature, reversible protein phosphorylations are sensitive to changes in the tissue environment. Stabilizor T1 is a system for rapid inactivation of enzymatic activity in biological samples. Enzyme inactivation is accomplished using thermal denaturation in a rapid, homogeneous, and reproducible fashion without the need for added inhibitors. Using pCREB(Ser133) as a model system, the applicability of the Stabilizor system to preserve a rapidly lost phosphorylation is shown.
authors.affiliation: Denator AB, Dag Hammarskjöldsv. 10A, Uppsala, Sweden. mats.boren@denator.com
authors.forename: Mats
authors.initials: M
authors.lastname: Borén
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007120:Immunochemistry / Q000379:methods*; D051379:Mice; D010766:Phosphorylation; D055550:Protein Stability; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D015398:Signal Transduction; D013048:Specimen Handling / Q000295:instrumentation*; D013696:Temperature
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D011506:Proteins