title: Dye-doped silica nanoparticles as efficient labels for glycans.
abstract: We report that dye-doped fluorescent silica nanoparticles (FSNPs) are highly efficient labels for glycans. Mono- and oligo-saccharides were conjugated to FSNPs using a general photocoupling chemistry. FSNP-labeled glycans were applied to image and detect bacteria, and to study carbohydrate-lectin interactions on a lectin microarray.
authors.affiliation: Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon 97207-0751, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Olof
authors.initials: O
authors.lastname: Ramström
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
mesh_terms: D001386:Azides / Q000737:chemistry; D001419:Bacteria / Q000302:isolation & purification; D019793:Fluorescein / Q000737:chemistry*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry; D037102:Lectins / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001386:Azides; D006845:Hydrocarbons, Fluorinated; D037102:Lectins; D011134:Polysaccharides; C556136:perfluorophenyl azide; D012822:Silicon Dioxide; D019793:Fluorescein