title: Detection of advanced oxidation protein products in patients with chronic kidney disease by a novel monoclonal antibody.
abstract: Advanced oxidation protein products (AOPP) as a biomarker of oxidative stress has been demonstrated in chronic kidney disease (CKD) patients; however, current methods to detect the accumulation of AOPP in serum and in tissues are limited and unreliable. This study generated a monoclonal antibody (mAb) designated 3F2, that reacts specifically with hypochlorous acid (HOCl)-modified proteins, but not with the native forms or with other types of oxidative modifications. Notably, mAb 3F2 recognizes the AOPP deposited in renal tissues of AOPP-treated rats and of patients with different kinds of CKD. Moreover, this mAb can almost completely inhibit the production of reactive oxygen species in RAW264.7 cells induced by AOPP (p < 0.001). In conclusion, mAb 3F2 can be used to detect AOPP specifically in serum and in tissues, and this antibody can potentially provide an important tool and new insight into research on diseases related to oxidative stress.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Beiyi
authors.initials: B
authors.lastname: Liu
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Hou
authors.forename: Qiugen
authors.initials: Q
authors.lastname: Zhou
authors.forename: Jianwei
authors.initials: J
authors.lastname: Tian
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Fanfan
authors.initials: F
authors.lastname: Hou
authors.forename: Ning
authors.initials: N
authors.lastname: Fu
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000918:Antibody Specificity; D001667:Binding, Competitive; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007668:Kidney / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000175:diagnosis* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D017382:Reactive Oxygen Species