title: RNA extraction by the proteinase k method.
abstract: This method of RNA extraction relies on a relatively gentle lysis procedure that should burst the cells, but leave the nuclei intact. Contamination of a relatively RNase-free cytoplasmic environment with nuclear nucleases is thus minimised. Next the polysomes are dissociated with SDS and proteinase K and finally the protein is removed by several phenol/choloroform extractions (1,2).
authors.affiliation: Inveresk Research International Limited, Musselburgh, Scotland.
authors.forename: R
authors.initials: R
authors.lastname: McGookin