title: Host inhibits replication of European porcine reproductive and respiratory syndrome virus in macrophages by altering differential regulation of type-I interferon transcriptional response.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease caused by a positive RNA strand arterivirus. PRRS virus (PRRSV) interacts primarily with lung macrophages. Little is known how the virus subverts the innate immune response to initiate its replication in alveolar macrophages. Large-scale transcriptional responses of macrophages with different levels of susceptibility to PRRSV infection were compared over 30 h of infection. This study demonstrates a rapid and intense host transcriptional remodelling during the early phase of the replication of the virus which correlates with transient repression of type-I interferon transcript as early as 8 h post-infection. These results support the suggestion from previous studies that host innate immune response inhibits replication of European porcine reproductive and respiratory syndrome virus in macrophages by altering differential regulation of type-I interferon transcriptional response.
authors.affiliation: The Roslin Institute and Royal Dick School of Veterinary Studies, The University of Edinburgh, Roslin, Midlothian EH25 9RG, UK. tahar.aitali@roslin.ed.ac.uk
authors.forename: Tahar
authors.initials: T
authors.lastname: Ait-Ali
authors.forename: Alison D
authors.initials: AD
authors.lastname: Wilson
authors.forename: Wilfrid
authors.initials: W
authors.lastname: Carré
authors.forename: David G
authors.initials: DG
authors.lastname: Westcott
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Frossard
authors.forename: Marnie A
authors.initials: MA
authors.lastname: Mellencamp
authors.forename: Daphne
authors.initials: D
authors.lastname: Mouzaki
authors.forename: Oswald
authors.initials: O
authors.lastname: Matika
authors.forename: David
authors.initials: D
authors.lastname: Waddington
authors.forename: Trevor W
authors.initials: TW
authors.lastname: Drew
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Bishop
authors.forename: Alan L
authors.initials: AL
authors.lastname: Archibald
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics; D007370:Interferon Type I / Q000235:genetics*; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000235:genetics / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000502:physiology*; D013552:Swine; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D007370:Interferon Type I