title: Quantification of plasma lactate concentrations using capillary electrophoresis with contactless conductivity detection.
abstract: The quantification of plasma lactate and evaluation of the lactate threshold by CE with capacitively coupled contactless conductivity is demonstrated. The only sample preparation needed was deproteinization with a ACN/methanol mixture. A solution of 10 mmol/L 2-morpholinoethanesulfonic acid monohydrate, 10 mmol/L DL-histidine, 70 μmol/L hexadecyltrimethylammonium bromide, pH 6.0 was found suitable as running buffer. Linearity was achieved for the concentration range of 10-1000 μmol/L with a correlation coefficient of 0.9994. The limit of detection (3 S/N) was determined as 3.2 μmol/L. Intra- and inter-day variabilities were less than 7% RSD. The suitability of the method could be demonstrated by analyzing various clinical samples, where the results correlated satisfactorily with those of an established enzymatic method.
authors.affiliation: Department of Chemistry, University of Basel, Basel, Switzerland.
authors.forename: Worapan
authors.initials: W
authors.lastname: Pormsila
authors.forename: Réjane
authors.initials: R
authors.lastname: Morand
authors.forename: Stephan
authors.initials: S
authors.lastname: Krähenbühl
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hauser
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D015444:Exercise / Q000502:physiology; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000097:Acetonitriles; D019344:Lactic Acid; D000432:Methanol; C032159:acetonitrile