title: The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis.
abstract: Many multicellular organisms have remarkable capability to regenerate new organs after wounding. As a first step of organ regeneration, adult somatic cells often dedifferentiate to reacquire cell proliferation potential, but mechanisms underlying this process remain unknown in plants. Here we show that an AP2/ERF transcription factor, WOUND INDUCED DEDIFFERENTIATION 1 (WIND1), is involved in the control of cell dedifferentiation in Arabidopsis. WIND1 is rapidly induced at the wound site, and it promotes cell dedifferentiation and subsequent cell proliferation to form a mass of pluripotent cells termed callus. We further demonstrate that ectopic overexpression of WIND1 is sufficient to establish and maintain the dedifferentiated status of somatic cells without exogenous auxin and cytokinin, two plant hormones that are normally required for cell dedifferentiation. In vivo imaging of a synthetic cytokinin reporter reveals that wounding upregulates the B-type ARABIDOPSIS RESPONSE REGULATOR (ARR)-mediated cytokinin response and that WIND1 acts via the ARR-dependent signaling pathway to promote cell dedifferentiation. This study provides novel molecular insights into how plants control cell dedifferentiation in response to wounding.
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8562, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Iwase
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.forename: Tomotsugu
authors.initials: T
authors.lastname: Koyama
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Hiratsu
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kojima
authors.forename: Takashi
authors.initials: T
authors.lastname: Arai
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Inoue
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sakakibara
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D054337:Cell Dedifferentiation / Q000502:physiology*; D049109:Cell Proliferation; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism*; D046228:Microarray Analysis; D008853:Microscopy; D009687:Nuclear Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C499534:APETALA2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C559637:WIND1 protein, Arabidopsis