title: Evaluation of an immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in clinical isolates.
abstract: Identification of Mycobacterium tuberculosis complex (MTC) remains slow. Over the years, several new technologies have been proposed to accelerate and simplify the detection of MTC. In this context, we evaluated an immunochromatographic assay (ICA) (BIO-LINE SD Ag MPT64 TB) for rapid identification of MTC, based on detection of a specific MPT64 antigen of MTC. We have tested it on i) mycobacterial cultures: 210 MTC strains and 28 nontuberculous mycobacteria; ii) M. bovis bacille Calmette-Guérin strain SSI (Statens Serum Institut, Denmark); and iii) 22 microorganisms other than mycobacteria, isolated from cultures. We concluded that this kit has an excellent specificity (100%) and sensitivity (99%) from isolated cultures. The ICA (BIO-LINE SD Ag MPT64 TB) allows excellent MTC identification from clinical isolates. It is a rapid, simple, and inexpensive test, and has a definite contribution in the rapid laboratory diagnosis of tuberculosis.
authors.affiliation: Laboratory of Microbiology and Immunology, UR02-SP13, Universitary Hospital Farhat Hached, Sousse 4000, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Marzouk
authors.forename: Imen Ben
authors.initials: IB
authors.lastname: Kahla
authors.forename: Naila
authors.initials: N
authors.lastname: Hannachi
authors.forename: Asma
authors.initials: A
authors.lastname: Ferjeni
authors.forename: Walid Ben
authors.initials: WB
authors.lastname: Salma
authors.forename: Samira
authors.initials: S
authors.lastname: Ghezal
authors.forename: Jalel
authors.initials: J
authors.lastname: Boukadida
mesh_terms: D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques; D009161:Mycobacterium / Q000145:classification / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080865:MPB64 protein, Mycobacterium; D011933:Reagent Kits, Diagnostic