title: Fluorescent labeling of degradable poly(lactide-co-glycolide) for cellular nanoparticles tracking in living cells.
abstract: Fluorescent-labeled aliphatic polyesters are essential materials for in vitro and in vivo studies of the behavior of these biodegradable polymers in interaction with cells or in a body. In particular, the direct cellular localization of drug delivery systems based on these materials allows better understanding of the internalization mechanism and determination of the pharmacokinetics. Polylactide-co-glycolide (PLGA) is a rapidly degradable copolymer widely used in pharmaceutics and nanomedecine. It was prepared by ring-opening polymerization of lactide and glycolide in order to obtain a well-defined material to investigate conditions allowing the covalent linkage of a fluorescent dye (fluorescein) while preserving the macromolecular characteristics of the polymer. The success of the functionalization was ascertained by proton nuclear magnetic resonance (1H NMR), size-exclusion chromatography (SEC) and fluorescence spectroscopy.
authors.affiliation: Center for Education and Research on Macromolecules, University of Liège, Liège, Belgium.
authors.forename: Hélène
authors.initials: H
authors.lastname: Freichels
authors.forename: Fabienne
authors.initials: F
authors.lastname: Danhier
authors.forename: Véronique
authors.initials: V
authors.lastname: Préat
authors.forename: Philippe
authors.initials: P
authors.lastname: Lecomte
authors.forename: Christine
authors.initials: C
authors.lastname: Jérôme
mesh_terms: D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D042783:Endothelial Cells / Q000378:metabolism*; D019793:Fluorescein / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D053758:Nanoparticles; D011098:Polyglactin 910 / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011098:Polyglactin 910; D019793:Fluorescein