title: Hemispheric comparisons of neuron density in the planum temporale of schizophrenia and nonpsychiatric brains.
abstract: Postmortem and in vivo studies of schizophrenia frequently reveal reduced cortical volume, but the underlying cellular abnormalities are incompletely defined. One influential hypothesis, especially investigated in Brodmann's area 9 of prefrontal cortex, is that the number of neurons is normal, and the volume change is caused by reduction of the surrounding neuropil. However, studies have differed on whether the cortex has the increased neuron density that is predicted by this hypothesis. In a recent study of bilateral planum temporale (PT), we reported smaller volume and width of the outer cortex (layers I-III), especially in the left hemisphere, among subjects with schizophrenia. In the present study, we measured neuron density and size in the same PT samples, and also in prefrontal area 9 of the same brains. In the PT, separate stereological measurements were made in layers II, IIIc, and VI, whereas area 9 was sampled in layer IIIb-c. In both cortical regions, there was no significant effect of schizophrenia on neuronal density or size. There was, nevertheless, a trend-level right>left hemispheric asymmetry of neuron density in the PT, which may partially explain the previously reported left>right asymmetry of cortical width. In schizophrenia, our findings suggest that closer packing of neurons may not always explain reduced cortical volume, and subtly decreased neuron number may be a contributing factor.
authors.affiliation: Nathan Kline Institute for Psychiatric Research, Orangeburg, NY, USA. smiley@nki.rfmh.org
authors.forename: John F
authors.initials: JF
authors.lastname: Smiley
authors.forename: Gorazd
authors.initials: G
authors.lastname: Rosoklija
authors.forename: Branislav
authors.initials: B
authors.lastname: Mancevski
authors.forename: Denise
authors.initials: D
authors.lastname: Pergolizzi
authors.forename: Khadija
authors.initials: K
authors.lastname: Figarsky
authors.forename: Cynthia
authors.initials: C
authors.lastname: Bleiwas
authors.forename: Aleksej
authors.initials: A
authors.lastname: Duma
authors.forename: J John
authors.initials: JJ
authors.lastname: Mann
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Javitt
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Dwork
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001931:Brain Mapping; D002452:Cell Count; D007839:Functional Laterality / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000473:pathology*; D019581:Neuropil / Q000473:pathology; D017397:Prefrontal Cortex / Q000473:pathology*; D012559:Schizophrenia / Q000473:pathology*; D013238:Stereotaxic Techniques