title: Is MLC phosphorylation essential for the recovery from ROCK inhibition in glioma C6 cells?
abstract: Inhibition of Rho-associated protein kinase (ROCK) activity in glioma C6 cells induces changes in actin cytoskeleton organization and cell morphology similar to those observed in other types of cells with inhibited RhoA/ROCK signaling pathway. We show that phosphorylation of myosin light chains (MLC) induced by P2Y₂ receptor stimulation in cells with blocked ROCK correlates in time with actin cytoskeleton reorganization, F-actin redistribution and stress fibers assembly followed by recovery of normal cell morphology. Presented results indicate that myosin light-chain kinase (MLCK) is responsible for the observed phosphorylation of MLC. We also found that the changes induced by P2Y₂ stimulation in actin cytoskeleton dynamics and morphology of cells with inhibited ROCK, but not in the level of phosphorylated MLC, depend on the presence of calcium in the cell environment.
authors.affiliation: Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Korczyński
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Sobierajska
authors.forename: Patryk
authors.initials: P
authors.lastname: Krzemiński
authors.forename: Anna
authors.initials: A
authors.lastname: Wasik
authors.forename: Dorota
authors.initials: D
authors.lastname: Wypych
authors.forename: Paweł
authors.initials: P
authors.lastname: Pomorski
authors.forename: Wanda
authors.initials: W
authors.lastname: Kłopocka
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D018994:Myosin Light Chains / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D058473:Receptors, Purinergic P2Y2 / Q000378:metabolism; D014544:Uridine Triphosphate / Q000494:pharmacology; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D000199:Actins; D018994:Myosin Light Chains; D058473:Receptors, Purinergic P2Y2; D054460:rho-Associated Kinases; D002118:Calcium; D014544:Uridine Triphosphate