title: Determination of soluble tumor necrosis factor-α receptor type I (TNFRI) and II (TNFRII) in the urine of healthy Japanese subjects.
abstract: We determined the urinary soluble tumor necrosis factor-α receptor type I (sTNFRI) and type II (sTNFRII) levels in healthy Japanese individuals to establish a reference value by means of specific ELISA. It was found that there were no significant differences between the urine sTNFRI and sTNFRII levels of children and adults. To demonstrate the usefulness of the reference value for children, we measured the urine sTNFRI and sTNFRII levels of children with diarrhea positive (D+) hemolytic uremic syndrome (HUS) as a preliminary study. The urine sTNFRI and sTNFRII levels of the patients with HUS were markedly higher than those of healthy children from the onset of D + HUS. Our reliable reference value for healthy children will allow us to discriminate between normal and pathological conditions in future studies.
authors.affiliation: Laboratory of Immunology and Microbiology, Faculty of Pharmacy, Chiba Institute of Science, Chiba, Japan. yokamoto@cis.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Mayuri
authors.initials: M
authors.lastname: Tanaka
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Gotoh
authors.forename: Naoya
authors.initials: N
authors.lastname: Fujita
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukui
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Masuzawa
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000652:urine*; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000652:urine*; D012995:Solubility
chemical_list: D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II