title: Nucleic acids extraction from laser microdissected FFPE tissue sections.
abstract: Tissue heterogeneity is a common source of unsuccessful experiments. Laser capture microdissection is a tool to prepare homogeneous tissue and cell areas as starting material for reliable and reproducible results as it allows the defined investigation of spatially different tissue areas.Nearly all samples allow the extraction of DNA. Fresh or fresh frozen samples are an ideal source for getting access to high-quality RNA. But also the large archives of formalin-fixed, paraffin-embedded (FFPE) tissue specimens are a valuable source of sample material for RNA extraction. Optimized protocols may help to make the RNA from FFPE material suitable for expression studies.
authors.affiliation: Carl Zeiss MicroImaging, München, Germany. burgemeister@zeiss.de
authors.forename: Renate
authors.initials: R
authors.lastname: Burgemeister
mesh_terms: D000269:Adhesives; D004247:DNA / Q000302:isolation & purification; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D008567:Membranes, Artificial; D042282:Microdissection / Q000379:methods*; D009696:Nucleic Acids / Q000302:isolation & purification*; D016612:Paraffin Embedding / Q000379:methods*; D011107:Polylysine / Q000494:pharmacology; D011786:Quality Control; D012313:RNA / Q000302:isolation & purification; D013194:Staining and Labeling; D016707:Tissue Fixation / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D000269:Adhesives; D008567:Membranes, Artificial; D009696:Nucleic Acids; D005557:Formaldehyde; D011107:Polylysine; D012313:RNA; D004247:DNA