title: Effect of narrow-band ultraviolet B phototherapy on p63 and microRNA (miR-21 and miR-125b) expression in psoriatic epidermis.
abstract: Psoriasis is an inflammatory skin disease in which dysregulation of p63, a member of the p53 family that is crucial for skin development and maintenance, has been demonstrated. Involvement of miR-203, miR-21 and miR-125b, small non-coding RNAs implicated in the regulation of p63 or p53, has been suggested in the patho-genesis of psoriasis. To elucidate the roles of p63 and p63-related microRNAs in psoriasis and to increase our understanding of the mechanisms of narrow-band ultraviolet B (NB-UVB) phototherapy, we studied the effects of NB-UVB treatment on the expression of these molecules. Skin biopsies from 12 psoriasis patients were collected before, during and after NB-UVB therapy. Real-time PCR and immunohistochemistry showed that p63 expression was not significantly affected, whereas NB-UVB phototherapy significantly decreased expression of miR-21 (p = 0.003) and increased miR-125b levels (p = 0.003). The results indicate that the unresolved p63 abnormality in treated epidermis may play a role in maintenance of this disease.
authors.affiliation: Department of Medical Biosciences/Pathology, Umeå University, Sweden.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Gu
authors.forename: Elisabet
authors.initials: E
authors.lastname: Nylander
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Coates
authors.forename: Karin
authors.initials: K
authors.lastname: Nylander
mesh_terms: D001706:Biopsy; D016022:Case-Control Studies; D004817:Epidermis / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000378:metabolism*; D011565:Psoriasis / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013548:Sweden; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016896:Treatment Outcome; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D014467:Ultraviolet Therapy
chemical_list: C531888:MIRN125 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins