title: Heterologous expression of transaldolase gene Tal from Saccharomyces cerevisiae in Fusarium oxysporum for enhanced bioethanol production.
abstract: The filamentous fungus Fusarium oxysporum is known for its ability to ferment xylose-producing ethanol. However, efficiency of xylose utilization and ethanol yield was low. In this study, the transaldolase gene from Saccharomyces cerevisiae has been successfully expressed in F. oxysporum by an Agrobacterium tumefaciens-mediated transformation method. The enzymatic activity of the recombinant fungus (cs28pCAM-Sctal4) was 0.195 times higher than that of the wild-type strain (cs28). The recombinant strain also exhibited a 28.83% increase in ethanol yield on xylose media compared to the parental strain. Enhanced ethanol production and a reduction in the biomass were observed during xylose fermentation. Ethanol yield from rice straw by simultaneous saccharification and fermentation with cs28pCAM-Sctal4 was 0.25 g g⁻¹ of rice straw. The transgenic strain of F. oxysporum cs28pCAM-Sctal4 might therefore have potential applications in industrial bioenergy production.
authors.affiliation: Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Jin-Xia
authors.initials: JX
authors.lastname: Fan
authors.forename: Xiao-Xue
authors.initials: XX
authors.lastname: Yang
authors.forename: Jin-Zhu
authors.initials: JZ
authors.lastname: Song
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Huang
authors.forename: Zhong-Xiang
authors.initials: ZX
authors.lastname: Cheng
authors.forename: Lin
authors.initials: L
authors.lastname: Yao
authors.forename: Olivia S
authors.initials: OS
authors.lastname: Juba
authors.forename: Qing
authors.initials: Q
authors.lastname: Liang
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Margaret
authors.initials: M
authors.lastname: Odeph
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D012275:Oryza / Q000378:metabolism; D010802:Phylogeny; D010957:Plasmids; D015202:Protein Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D014153:Transaldolase / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005656:Fungal Proteins; D000431:Ethanol; D014994:Xylose; D014153:Transaldolase; D005947:Glucose