title: Fragment screening of infectious disease targets in a structural genomics environment.
abstract: Structural genomics efforts have traditionally focused on generating single protein structures of unique and diverse targets. However, a lone structure for a given target is often insufficient to firmly assign function or to drive drug discovery. As part of the Seattle Structural Genomics Center for Infectious Disease (SSGCID), we seek to expand the focus of structural genomics by elucidating ensembles of structures that examine small molecule-protein interactions for selected infectious disease targets. In this chapter, we discuss two applications for small molecule libraries in structural genomics: unbiased fragment screening, to provide inspiration for lead development, and targeted, knowledge-based screening, to confirm or correct the functional annotation of a given gene product. This shift in emphasis results in a structural genomics effort that is more engaged with the infectious disease research community, and one that produces structures of greater utility to researchers interested in both protein function and inhibitor development. We also describe specific methods for conducting high-throughput fragment screening in a structural genomics context by X-ray crystallography.
authors.affiliation: Emerald BioStructures, Bainbridge Island, Washington, USA.
authors.forename: Darren W
authors.initials: DW
authors.lastname: Begley
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Davies
authors.forename: Robert C
authors.initials: RC
authors.lastname: Hartley
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Edwards
authors.forename: Bart L
authors.initials: BL
authors.lastname: Staker
authors.forename: Wesley C
authors.initials: WC
authors.lastname: Van Voorhis
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
authors.forename: Lance J
authors.initials: LJ
authors.lastname: Stewart
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003141:Communicable Diseases / Q000188:drug therapy*; D019295:Computational Biology; D003460:Crystallization; D018360:Crystallography, X-Ray; D055808:Drug Discovery / Q000379:methods*; D023281:Genomics / Q000379:methods*; D008024:Ligands; D019761:Phosphorus-Oxygen Lyases / Q000037:antagonists & inhibitors; D054852:Small Molecule Libraries
chemical_list: D001426:Bacterial Proteins; D008024:Ligands; D054852:Small Molecule Libraries; D019761:Phosphorus-Oxygen Lyases