title: Spoxazomicins A-C, novel antitrypanosomal alkaloids produced by an endophytic actinomycete, Streptosporangium oxazolinicum K07-0460(T).
abstract: Three novel antitrypanosomal alkaloids, named spoxazomicins A-C, were isolated by silica gel column chromatography and HPLC from the culture broth of a new endophytic actinomycete species, Streptosporangium oxazolinicum K07-0460(T). The structures of the spoxazomicins were elucidated by NMR and X-ray crystal analyses and shown to be new types of pyochelin family antibiotic. Spoxazomicin A showed potent and selective antitrypanosomal activity with an IC₅₀ value of 0.11 μg ml⁻¹ in vitro without cytotoxicity against MRC-5 cells (IC₅₀=27.8 μg ml⁻¹).
authors.affiliation: Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inahashi
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Aki
authors.initials: A
authors.lastname: Ishiyama
authors.forename: Miyuki
authors.initials: M
authors.lastname: Namatame
authors.forename: Aki
authors.initials: A
authors.lastname: Nishihara-Tsukashima
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Hirose
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Sunazuka
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Otoguro
authors.forename: Yōko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D020128:Inhibitory Concentration 50; D010080:Oxazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D058428:Silica Gel; D013844:Thiazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D010080:Oxazoles; D010636:Phenols; D013844:Thiazoles; D014344:Trypanocidal Agents; C558839:spoxazomicin A; C558840:spoxazomicin B; C558841:spoxazomicin C; D058428:Silica Gel; C025316:pyochelin