title: Aliidiomarina taiwanensis gen. nov., sp. nov., isolated from shallow coastal water.
abstract: A Gram-negative, heterotrophic, aerobic, marine bacterium, designated AIT1(T), was isolated from a seawater sample collected in the shallow coastal region of Bitou Harbour, New Taipei City, Taiwan. Cells grown in broth cultures were straight or slightly curved rods that were motile by means of a single polar flagellum. Strain AIT1(T) required NaCl for growth, grew optimally at 30-40°C and with 1.5-5.0% NaCl, and was incapable of anaerobic growth by fermentation of glucose or other carbohydrates. The isoprenoid quinones consisted of Q-8 (95.2%) and Q-9 (4.8%). The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cellular fatty acids were predominantly iso-branched and included iso-C(17:0) (26.5%), summed feature 9 (comprising iso-C(17:1)ω9c and/or 10-methyl C(16:0); 25.9%) and iso-C(15:0) (20.5%). The DNA G+C content was 51.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AIT1(T) formed a distinct lineage within the class Gammaproteobacteria and was most closely related to members of the genus Idiomarina in the family Idiomarinaceae (91.5-93.9% 16S rRNA gene sequence similarity). The phylogenetic data, together with chemotaxonomic, physiological and morphological data, revealed that the isolate should be classified as a representative of a novel species in a new genus in the family Idiomarinaceae, for which the name Aliidiomarina taiwanensis gen. nov., sp. nov. is proposed. The type strain is AIT1(T) (=JCM 16052(T)=BCRC 80035(T)=NCCB 100321(T)).
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, Republic of China.
authors.forename: Ssu-Po
authors.initials: SP
authors.lastname: Huang
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, Republic of China.
authors.forename: Hsiao-Yun
authors.initials: HY
authors.lastname: Chang
authors.affiliation: College of Health Care, China Medical University, No. 91, Shyue-Shyh Rd, Taichung, Taiwan, Republic of China.
authors.forename: Jwo-Sheng
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Center for General Education, University of Kang Ning, No. 188, Sec. 5, An-Chung Rd, Tainan, Taiwan, Republic of China.
authors.forename: Wen Dar
authors.initials: WD
authors.lastname: Jean
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, Republic of China.
authors.forename: Wung Yang
authors.initials: WY
authors.lastname: Shieh
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008124:Locomotion; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013624:Taiwan; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride