title: Implementation of 89Zr production and in vivo imaging of B-cells in mice with 89Zr-labeled anti-B-cell antibodies by small animal PET/CT.
abstract: We examined the production, separation, and characterization of (89)Zr, including supplementation of a commercial Cyclone(®) 18/9 with a self-made Solid Target System (STS). Obtained [(89)Zr]Zr-oxalate was used for the labeling of anti-B cell antibodies with desferrioxamine-p-SCN as a bifunctional chelator. (89)Zr-labeled antibodies were injected in DBA/1 mice to examine usability for detection of B cells in vivo. PET measurements showed binding of (89)Zr-labeled anti-B cell antibodies in tissues with high frequencies of B cells, i.e. in spleen and lymph nodes.
authors.affiliation: Institute of Radiopharmacy, Helmholtz-Zentrum Dresden-Rossendorf, Germany. m.walther@hzdr.de
authors.forename: Martin
authors.initials: M
authors.lastname: Walther
authors.forename: Peter
authors.initials: P
authors.lastname: Gebhardt
authors.forename: Philipp
authors.initials: P
authors.lastname: Grosse-Gehling
authors.forename: Lydia
authors.initials: L
authors.lastname: Würbach
authors.forename: Ingo
authors.initials: I
authors.lastname: Irmler
authors.forename: Stephan
authors.initials: S
authors.lastname: Preusche
authors.forename: Muhammad
authors.initials: M
authors.lastname: Khalid
authors.forename: Thomas
authors.initials: T
authors.lastname: Opfermann
authors.forename: Thomas
authors.initials: T
authors.lastname: Kamradt
authors.forename: Jörg
authors.initials: J
authors.lastname: Steinbach
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Saluz
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000737:chemistry; D001402:B-Lymphocytes / Q000000981:diagnostic imaging* / Q000276:immunology / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry; D003676:Deferoxamine / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D007553:Isotope Labeling; D008198:Lymph Nodes / Q000166:cytology / Q000000981:diagnostic imaging; D051379:Mice; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D049268:Positron-Emission Tomography / Q000379:methods; D011810:Quinoxalines / Q000737:chemistry; D011859:Radiography; D016719:Radioimmunodetection / Q000379:methods*; D011868:Radioisotopes / Q000032:analysis* / Q000737:chemistry; D013051:Spectrometry, Gamma / Q000379:methods*; D013154:Spleen / Q000166:cytology / Q000000981:diagnostic imaging; D014018:Tissue Distribution; D015040:Zirconium / Q000032:analysis* / Q000737:chemistry
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D007211:Indoles; D011810:Quinoxalines; D011868:Radioisotopes; C060183:B 220; D015040:Zirconium; D003676:Deferoxamine