title: A stereoselective vanadium-dependent chloroperoxidase in bacterial antibiotic biosynthesis.
abstract: Halogenases catalyze reactions that introduce halogen atoms into electron-rich organic molecules. Vanadium-dependent haloperoxidases are generally considered to be promiscuous halogenating enzymes that have thus far been derived exclusively from eukaryotes, where their cellular function is often disputed. We now report the first biochemical characterization of a bacterial vanadium-dependent chloroperoxidase, NapH1 from Streptomyces sp. CNQ-525, which catalyzes a highly stereoselective chlorination-cyclization reaction in napyradiomycin antibiotic biosynthesis. This finding biochemically links a vanadium chloroperoxidase to microbial natural product biosynthesis.
authors.affiliation: Scripps Institution of Oceanography, University of California, San Diego, California 92093, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Bernhardt
authors.forename: Tatsufumi
authors.initials: T
authors.lastname: Okino
authors.forename: Jaclyn M
authors.initials: JM
authors.lastname: Winter
authors.forename: Akimasa
authors.initials: A
authors.lastname: Miyanaga
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Moore
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D055162:Biocatalysis; D002711:Chloride Peroxidase / Q000737:chemistry / Q000378:metabolism*; D003500:Cyclization; D015394:Molecular Structure; D009285:Naphthoquinones / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014639:Vanadium / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D009285:Naphthoquinones; D014639:Vanadium; D002711:Chloride Peroxidase