title: Influence of K+ and Na+ ions on the aggregation processes of antibiotic amphotericin B: electronic absorption and FTIR spectroscopic studies.
abstract: The ionophore properties of amphotericin B (AmB) are related to the transport of Na(+) and K(+) ions across the molecular pores formed by this antibiotic in lipid membranes. In this paper, we present a new, complementary mechanism in which the -COO(-) group of the antibiotic is involved in the binding process of Na(+) and K(+) ions. Spectroscopic studies indicate that K(+) and Na(+) ions play an important role in the AmB aggregation process. Evidence in several spectral regions shows that K(+) ions exhibit a stronger ionic binding affinity to the -COO(-) group relative to Na(+). Overall, our findings indicate that monovalent ions can affect the molecular organization of AmB in substantially different ways not previously considered to be significant for their biological action.
authors.affiliation: Department of Biophysics, University of Life Sciences in Lublin, Lublin, Poland. mariusz.gagos@up.lublin.pl
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
authors.forename: Marta
authors.initials: M
authors.lastname: Arczewska
mesh_terms: D000042:Absorption; D000666:Amphotericin B / Q000737:chemistry* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D004583:Electrons; D011188:Potassium / Q000737:chemistry* / Q000378:metabolism; D012964:Sodium / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D000666:Amphotericin B; D012964:Sodium; D011188:Potassium