title: Mesenchymal stem cells in ex vivo cord blood expansion.
abstract: Umbilical cord blood (CB) is becoming an important source of haematopoietic support for transplant patients lacking human leukocyte antigen matched donors. The ethnic diversity, relative ease of collection, ready availability as cryopreserved units from CB banks, reduced incidence and severity of graft versus host disease and tolerance of higher degrees of HLA disparity between donor and recipient, are positive attributes when compared to bone marrow or cytokine-mobilized peripheral blood. However, CB transplantation is associated with significantly delayed neutrophil and platelet engraftment and an elevated risk of graft failure. These hurdles are thought to be due, at least in part, to low total nucleated cell and CD34(+) cell doses transplanted. Here, current strategies directed at improving TNC and CD34(+) cell doses at transplant are discussed, with particular attention paid to the use of a mesenchymal stem cell (MSC)/CB mononuclear cell ex vivo co-culture expansion system.
authors.affiliation: Department of Stem Cell Transplantation and Cellular Therapy, University of Texas, M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA. snrobins@mdanderson.org
authors.forename: Simon N
authors.initials: SN
authors.lastname: Robinson
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Simmons
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Amin M
authors.initials: AM
authors.lastname: Alousi
authors.forename: J
authors.initials: J
authors.lastname: Marcos de Lima
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Shpall
mesh_terms: D000328:Adult; D018952:Antigens, CD34; D002448:Cell Adhesion; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D002648:Child; D018920:Coculture Techniques; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology*; D006085:Graft Survival; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010969:Plastics
chemical_list: D018952:Antigens, CD34; D010969:Plastics