title: Investigational study of tamoxifen phase I metabolites using chromatographic and spectroscopic analytical techniques.
abstract: A comprehensive overview is presented of currently known phase I metabolites of tamoxifen consisting of their systematic name and molecular structure. Reference standards are utilized to elucidate the MS(n) fragmentation patterns of these metabolites using a linear ion trap mass spectrometer. UV-absorption spectra are recorded and absorption maxima are defined. Serum extracts from ten breast cancer patients receiving 40mg tamoxifen once daily were qualitatively analyzed for tamoxifen phase I metabolites using a liquid chromatography-tandem mass spectrometry set-up. In total, 19 metabolites have been identified in these serum samples. Additionally a synthetic method for the preparation of the putative metabolite 3',4'-dihydroxytamoxifen is described.
authors.affiliation: Department of Pharmacy & Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066 EC, Amsterdam, The Netherlands. Bas.Teunissen@slz.nl
authors.forename: S F
authors.initials: SF
authors.lastname: Teunissen
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: M Dominguez
authors.initials: MD
authors.lastname: Seoane
authors.forename: L
authors.initials: L
authors.lastname: Brunsveld
authors.forename: J H M
authors.initials: JH
authors.lastname: Schellens
authors.forename: A H
authors.initials: AH
authors.lastname: Schinkel
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
mesh_terms: D018931:Antineoplastic Agents, Hormonal / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D001943:Breast Neoplasms / Q000097:blood / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D050216:Metabolic Detoxication, Phase I; D015394:Molecular Structure; D011809:Quinones / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D013629:Tamoxifen / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: C404884:3,4-dihydroxytamoxifen; D018931:Antineoplastic Agents, Hormonal; D011809:Quinones; D013629:Tamoxifen