title: Mg-protoporphyrin, haem and sugar signals double cellular total RNA against herbicide and high-light-derived oxidative stress.
abstract: Cellular total RNA level is usually stable, although it may increase gradually during growth or seed germination, or decrease gradually under environmental stresses. However, we found that plant cell RNA could be doubled within 48 h in response to herbicide-induced Mg-protoporphyrin and heme accumulation or a high level of sugar treatment. This rapid RNA multiplication is important for effective cellular resistance to oxidative stress, such as high-light and herbicide co-stress conditions, where the plastid-signalling defective mutant gun1 shows an apparent phenotype (more severe photobleaching). Hexokinase is required for sugar-induced RNA multiplication. While both sugar and Mg-protoporphyrin IX require plastid protein GUN1 and a nuclear transcription factor ABI4, haem appears to function through an independent pathway to control RNA multiplication. The transcription co-factor CAAT binding protein mediates the rapid RNA multiplication in plant cells in all the cases.
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Yang-Er
authors.initials: YE
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Mo-Yun
authors.initials: MY
authors.lastname: Xu
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Xue
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Xiao-Chao
authors.initials: XC
authors.lastname: Xu
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Lin
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000528:radiation effects; D002241:Carbohydrates / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics; D006418:Heme / Q000494:pharmacology*; D006540:Herbicides / Q000494:pharmacology*; D008027:Light; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D018087:Plastids / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D011524:Protoporphyrins / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018749:RNA, Plant / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D002241:Carbohydrates; D006540:Herbicides; D011524:Protoporphyrins; D011724:Pyridazines; D012333:RNA, Messenger; D018749:RNA, Plant; D012335:RNA, Ribosomal; C039629:magnesium protoporphyrin; D006418:Heme; D012343:RNA, Transfer; C008677:norflurazone