title: [Subtyping of lipooligosaccharides of non-typeable haemophilus influenzae isolated from children with bronchopulmonary diseases].
abstract: OBJECTIVE
  Subtyping of lipooligosaccharides (LOS) of non-typeable strains of Haemophilus influenzae (NTHi) isolated from children with bronchopulmonary diseases.
  
  
  METHODS
  Lipooligosaccharides obtained from 62 acapsular strains of H. influenzae were studied by vertical SDS-electrophoresis in PAAG.
  
  
  RESULTS
  Majority of LOS formed electrophoretically mobile components in low molecular mass zone. Obtained results allowed to differentiate 23 subtypes of LOS. Lipooligosaccharides of majority of strains (67.7%) belonged to one of 10 main subtypes, 30.6% of strains belonged to mixed subtypes because they had signs of 2-3 subtypes.
  
  
  CONCLUSIONS
  Strains possessing LOS of three subtypes--VI, VII, and X--were significantly more prevalent in pediatric patients (p < 0.05). More than one third (43.5%) of studied NTHi strains belonged to these subtypes.
authors.forename: N P
authors.initials: NP
authors.lastname: Vaneeva
authors.forename: O V
authors.initials: OV
authors.lastname: Novikova
authors.forename: O E
authors.initials: OE
authors.lastname: Orlova
authors.forename: N G
authors.initials: NG
authors.lastname: Kalina
authors.forename: S I
authors.initials: SI
authors.lastname: Elkina
authors.forename: M M
authors.initials: MM
authors.lastname: Tokarskaia
authors.forename: L K
authors.initials: LK
authors.lastname: Kotosova
authors.forename: N E
authors.initials: NE
authors.lastname: Iastrebova
mesh_terms: D000942:Antigens, Bacterial / Q000145:classification*; D015373:Bacterial Typing Techniques; D001982:Bronchial Diseases / Q000382:microbiology*; D002648:Child; D004586:Electrophoresis; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification* / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000145:classification*; D008171:Lung Diseases / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides