title: Effects of bioreactive acrolein from automotive exhaust gases on human cells in vitro.
abstract: Acrolein is a toxic unsaturated aldehyde and widespread environmental pollutant produced during lipid peroxidation and also by burning of tobacco or liquid fuels. Inhalation or dermal exposure to acrolein could be toxic to organisms. This very reactive aldehyde has a strong affinity for binding to proteins thus forming pathogenic protein-adducts. In the present study we have analyzed formation of bioreactive acrolein-protein adducts in bovine serum albumin solution exposed to exhaust gases of mineral diesel fuel and of mineral diesel fuel supplemented with different amounts of a novel diesel fuel additive denoted Ecodiesel (produced by a genuine procedure of recycling of plant oils used for food preparation). The effects of acrolein-protein adducts were tested on human microvascular endothelial cells and on human osteosarcoma cells that are sensitive to bioactivities of lipid peroxidation products. The results have shown a reduction of the bioreactive acrolein in exhaust gases when mineral diesel was supplemented with 5-20% Ecodiesel. Moreover, acrolein-protein adducts obtained from mineral diesel supplemented with Ecodiesel were less toxic than those obtained from mineral diesel alone. Thus, we assume that supplementing mineral diesel fuel with Ecodiesel would be of benefit for the use of renewable energy, for environment and for human health due to reduced environmental pollution with bioreactive acrolein.
authors.affiliation: Department of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia.
authors.forename: Morana
authors.initials: M
authors.lastname: Jaganjac
authors.forename: Iva Ozana
authors.initials: IO
authors.lastname: Prah
authors.forename: Ana
authors.initials: A
authors.lastname: Cipak
authors.forename: Marina
authors.initials: M
authors.lastname: Cindric
authors.forename: Lidija
authors.initials: L
authors.lastname: Mrakovcic
authors.forename: Franz
authors.initials: F
authors.lastname: Tatzber
authors.forename: Petar
authors.initials: P
authors.lastname: Ilincic
authors.forename: Vinko
authors.initials: V
authors.lastname: Rukavina
authors.forename: Branka
authors.initials: B
authors.lastname: Spehar
authors.forename: Jelena Parlov
authors.initials: JP
authors.lastname: Vukovic
authors.forename: Sanda
authors.initials: S
authors.lastname: Telen
authors.forename: Koji
authors.initials: K
authors.lastname: Uchida
authors.forename: Zoran
authors.initials: Z
authors.lastname: Lulic
authors.forename: Neven
authors.initials: N
authors.lastname: Zarkovic
mesh_terms: D000171:Acrolein / Q000633:toxicity*; D000818:Animals; D002417:Cattle; D002460:Cell Line; D004785:Environmental Pollutants / Q000633:toxicity*; D005742:Gasoline / Q000633:toxicity; D006801:Humans; D015227:Lipid Peroxidation; D011506:Proteins / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D004785:Environmental Pollutants; D005742:Gasoline; D011506:Proteins; D001335:Vehicle Emissions; D012710:Serum Albumin, Bovine; D000171:Acrolein