title: The chemical-in-μwell: a high-throughput technique for identifying solutes eliciting a chemotactic response in motile bacteria.
abstract: Bacteria, and in particular marine bacteria, can be found in environments that are poor in nutrients. To survive, they are able to move toward more favorable niches by a mechanism called chemotaxis, whose first step consists in the detection of substrates by chemoreceptors. We developed a chemotactic assay enabling rapid testing of several hundred different solutes and we identified several molecules eliciting a chemotactic response from two aquatic Shewanella species. We propose that this assay be used for other bacteria to determine the repertoire of chemotactic molecules, generally not clearly elucidated.
authors.affiliation: Laboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS-UPR 9043, 31 Chemin Joseph Aiguier, 13402 Marseille cedex 20, France. jarmitano@ifr88.cnrs-mrs.fr
authors.forename: Joshua
authors.initials: J
authors.lastname: Armitano
authors.forename: Claudine
authors.initials: C
authors.lastname: Baraquet
authors.forename: Valérie
authors.initials: V
authors.lastname: Michotey
authors.forename: Vincent
authors.initials: V
authors.lastname: Méjean
authors.forename: Cécile
authors.initials: C
authors.lastname: Jourlin-Castelli
mesh_terms: D018407:Bacterial Physiological Phenomena; D002628:Chemoreceptor Cells; D002630:Chemotactic Factors / Q000032:analysis* / Q000737:chemistry; D002633:Chemotaxis / Q000502:physiology*; D057166:High-Throughput Screening Assays; D020592:Shewanella / Q000502:physiology*
chemical_list: D002630:Chemotactic Factors