title: Modulation of T cell cytokine production by miR-144* with elevated expression in patients with pulmonary tuberculosis.
abstract: microRNAs have a critical role in regulating innate and adaptive immunity. To understand whether microRNAs play roles in regulating immune responses to Mycobacterium tuberculosis infection in humans, microRNA expression profiling was performed in PBMCs from pulmonary tuberculosis patients and healthy controls. Analysis of expression profiles showed that expression of 30 microRNAs was significantly altered during active TB as compared with healthy controls, 28 microRNAs were up-regulated and 2 microRNAs down-regulated. miR-144* was one of the microRNAs that were overexpressed in active TB patients. Real-time RT-PCR analysis showed that miR-144* was mainly expressed in T cells. Transfection of T cells with miR-144* precursor demonstrated that miR-144* could inhibit TNF-α and IFN-γ production and T cell proliferation. It is concluded that miR-144* might involve in regulation of anti-TB immunity through modification of cytokine production and cell proliferation of T cells.
authors.affiliation: Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Xinjing
authors.initials: X
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cao
authors.forename: Bingfen
authors.initials: B
authors.lastname: Yang
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Cheng
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D049109:Cell Proliferation; D016000:Cluster Analysis; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology*; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D016207:Cytokines; C554293:MIRN144 microRNA, human; D035683:MicroRNAs