title: Development and validation of an HPLC/MS/MS method for the determination of sufentanil and morphine in human plasma.
abstract: A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a C18 column and the mobile phase acetonitrile-5 mM ammonium acetate + 0.25% formic acid (70 + 30, v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source in positive mode was set up in the selective reaction monitoring mode to detect precursor --> product ion transition 387.0 > 238.0, 285.7 > 165.1, and 337.0 > 188.0 for sufentanil, morphine, and IS, respectively. The method was linear in the 0.05 (LOQ) - 500 ng/mL range for sufentanil and 10 (LOQ) - 1000 ng/mL range for morphine. Good selectivity, linearity, precision, accuracy, and robustness were obtained for the HPLC/MS/MS method. The proposed method was successfully applied for the determination of sufentanil and morphine in patients undergoing cardiac surgery.
authors.affiliation: School of Pharmaceutical Sciences USP/SP, Av. Lineu Prestes, 580 Bloco 13a, Butantã-Cidade Universitária, CEP 05508-900, São Paulo-SP Brazil. manfiojoselia@bol.com.br
authors.forename: Josélia Larger
authors.initials: JL
authors.lastname: Manfio
authors.forename: Verônica Jorge
authors.initials: VJ
authors.lastname: Santos
authors.forename: Vera Lúcia
authors.initials: VL
authors.lastname: Lanchote
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Santos
authors.forename: Maria José C
authors.initials: MJ
authors.lastname: Carmona
authors.forename: José Otavio Júnior
authors.initials: JO
authors.lastname: Auler
authors.forename: Liberato Júnior
authors.initials: LJ
authors.lastname: Brum
authors.forename: Carmen Maria
authors.initials: CM
authors.lastname: Donaduzzi
authors.forename: Silvia R C J
authors.initials: SR
authors.lastname: Santos
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood / Q000592:standards; D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards / Q000706:statistics & numerical data; D006348:Cardiac Surgical Procedures; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005283:Fentanyl / Q000097:blood / Q000592:standards; D006801:Humans; D009020:Morphine / Q000097:blood* / Q000592:standards; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D017409:Sufentanil / Q000097:blood* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D000701:Analgesics, Opioid; D009020:Morphine; D017409:Sufentanil; D005283:Fentanyl