title: [Analytical method of hydramethylnon in agricultural products by LC-MS/MS].
abstract: A simple determination method of hydramethylnon in agricultural products by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The sample was homogenized with phosphoric acid and extracted with acetone. An aliquot of crude extract was re-extracted with hexane and sat. NaCl solution. In the case of tea leaf, solidification processing using ammonium chloride and phosphoric acid was performed before re-extraction with hexane. Clean-up was performed using a silica-gel mini column (500 mg). The LC separation was performed on a C18 column using methanol-water (8 : 2) containing 10 mM ammonium acetate as the mobile phase and MS detection with positive ion electrospray ionization. The calibration curve was linear between 0.002-0.2 µg/mL of hydramethylnon. Recoveries (n=5) of hydromethylnon from 10 kinds of agricultural products fortified at 0.01 µg/g (0.05 µg/g for pineapple) were 82-110%, and their relative standard deviations were 2-12%.
authors.affiliation: Saitama Institute of Public Health, Sakura-ku, Saitama-shi, Saitama, Japan. takahashi.kunihiko@pref.saitama.lg.jp
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Ryuji
authors.initials: R
authors.lastname: Matsumoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Nemoto
authors.forename: Rieko
authors.initials: R
authors.lastname: Matsuda
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis*; D011744:Pyrimidinones / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007306:Insecticides; D011744:Pyrimidinones; C041642:hydramethylnon