title: Proteomic analysis of swine serum following highly virulent classical swine fever virus infection.
abstract: BACKGROUND
  Classical swine fever virus (CSFV) belongs to the genus Pestivirus within the family Flaviviridae. Virulent strains of classical swine fever virus (CSFV) cause severe disease in pigs characterized by immunosuppression, thrombocytopenia and disseminated intravascular coagulation, which causes significant economic losses to the pig industry worldwide.
  
  
  METHODS
  To reveal proteomic changes in swine serum during the acute stage of lethal CSFV infection, 5 of 10 pigs were inoculated with the virulent CSFV Shimen strain, the remainder serving as uninfected controls. A serum sample was taken at 3 days post-infection from each swine, at a stage when there were no clinical symptoms other than increased rectal temperatures (≥ 40 °C). The samples were treated to remove serum albumin and immunoglobulin (IgG), and then subjected to two-dimension differential gel electrophoresis.
  
  
  RESULTS
  Quantitative intensity analysis revealed 17 protein spots showing at least 1.5-fold quantitative alteration in expression. Ten spots were successfully identified by MALDI-TOF MS or LTQ MS. Expression of 4 proteins was increased and 6 decreased in CSFV-infected pigs. Functions of these proteins included blood coagulation, anti-inflammatory activity and angiogenesis.
  
  
  CONCLUSIONS
  These proteins with altered expression may have important implications in the pathogenesis of classical swine fever and provide a clue for identification of biomarkers for classical swine fever early diagnosis.
authors.affiliation: Institute of Biotechnology, College of Science, Northeastern University, Shenyang 110004, China.
authors.forename: Jin-fu
authors.initials: JF
authors.lastname: Sun
authors.forename: Zi-xue
authors.initials: ZX
authors.lastname: Shi
authors.forename: Huan-cheng
authors.initials: HC
authors.lastname: Guo
authors.forename: Su
authors.initials: S
authors.lastname: Li
authors.forename: Chang-chun
authors.initials: CC
authors.lastname: Tu
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006691:Classical Swine Fever / Q000097:blood* / Q000235:genetics / Q000378:metabolism / Q000821:virology; D006692:Classical Swine Fever Virus / Q000472:pathogenicity / Q000502:physiology*; D013058:Mass Spectrometry; D040901:Proteomics; D013552:Swine; D014774:Virulence
chemical_list: D001798:Blood Proteins