title: Immunohistochemical study of the expression of fatty acid synthase and Ki-67 in salivary gland tumors.
abstract: BACKGROUND
  The enzyme fatty acid synthase plays a fundamental role in the biosynthesis of fatty acids. Several recent studies have demonstrated a high fatty acid synthase expression in malignant tumors. Few studies have been conducted in oral and salivary gland tumors describing the fatty acid synthase expression.
  
  
  METHODS
  This study evaluated and compared, by immunohistochemical reaction, the expression of fatty acid synthase and Ki-67 in salivary gland tumors. The immunohistochemical study used the streptavidin-biotin-peroxidase technique, with antibodies anti-fatty acid synthase and anti-Ki-67. The fatty acid synthase was analyzed by scores, considering the intensity of labeling, quantity of labeled cells and histological component. The Ki-67 was analyzed by counting of one thousand cells, calculating the quantity of positive cells in regions with higher density of labeling. Statistical analysis was performed using the Pearson and Mann-Whitney correlation tests.
  
  
  RESULTS
  There was greater fatty acid synthase expression in pleomorphic adenoma compared to other tumors, and predominance of Ki-67 in malignant tumors. Among these, the mucoepidermoid carcinoma presented the highest proliferation rate. The expression of fatty acid synthase and Ki-67 did not present correlation between the tumors analyzed, except in pleomorphic adenomas, with statistically significant relationship between them.
  
  
  CONCLUSIONS
  It is suggested that the fat metabolism in salivary gland tumors is related to maintenance of cell differentiation. Its expression prevailed in benign tumors, while Ki-67 prevailed in the mucoepidermoid carcinoma, demonstrating its high proliferation rate, followed by the cystic adenoid carcinoma and polymorphous low grade adenocarcinoma.
authors.affiliation: Department of Physiotherapy, Superior School of Cruzeiro, Cruzeiro, São Paulo, Brazil. renatafalchete@hotmail.com
authors.forename: Renata Falchete
authors.initials: RF
authors.lastname: do Prado
authors.forename: Ana Lourdes
authors.initials: AL
authors.lastname: da Silva Machado
authors.forename: Carlos Eduardo Dias
authors.initials: CE
authors.lastname: Colombo
authors.forename: Yasmin Rodarte
authors.initials: YR
authors.lastname: Carvalho
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism / Q000473:pathology; D018277:Carcinoma, Mucoepidermoid / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation; D064429:Fatty Acid Synthases / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008297:Male; D009208:Myoepithelioma / Q000378:metabolism / Q000473:pathology; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D018709:Statistics, Nonparametric
chemical_list: D019394:Ki-67 Antigen; D064429:Fatty Acid Synthases