title: The effect of proteoglycans inhibited on the neurotropic growth of salivary adenoid cystic carcinoma.
abstract: OBJECTIVE
  To evaluate the relationship between inhibition of proteoglycans which secreted by salivary adenoid cystic carcinoma cell line (SACC-83) and the neurotropic ability of the tumor cells.
  
  
  METHODS
  The expression vector of short hairpin RNA (shRNA-WJ4) targeting xylosyltransferase-I gene was constructed and transfected into SACC-83 cells (group SACC83-WJ4), shRNA-HK used as negative control was transfected into SACC-83 cells (group SACC-83-HK), SACC-83 cells without transfection was used as black control (group SACC-83). The xylosyltransferase-I gene expression was measured by real-time PCR. The content of proteoglycans was detected by Blyscan Assay Kit. The effect of down-regulated proteoglycans on the perineural invasion of nude mice was observed. All data were analyzed by the software spss 13.0.
  
  
  RESULTS
  The results showed that the transfected efficiency of shRNA was 43.3%. The expression of xylosyltransferase-I was inhibited by 43.0% 48 h after transfection of shRNA-WJ4. The content of proteoglycans was down-regulated by 30.25% 48 h after transfection. In the neurotropic experiment in vivo of nude mice, the rate of perineural invasion of group SACC-83-WJ4 was 33.33%, significantly lower than that of the negative control (100%) and the black control (100%) (P < 0.05).
  
  
  CONCLUSIONS
  Xylosyltransferase-I gene of SACC cells was silenced by RNA interference technology, proteoglycans secretion was reduced and neurotropic invasion behavior of SACC was inhibited obviously.
authors.affiliation: Department of Oral Pathology, The Key Laboratory of Stomatology, College of Stomatology, Hebei Medical University, Hebei Province, China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Fusheng
authors.initials: F
authors.lastname: Dong
authors.forename: Hexiang
authors.initials: H
authors.lastname: Li
authors.forename: Yali
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010430:Pentosyltransferases / Q000235:genetics*; D010524:Peripheral Nervous System Neoplasms / Q000473:pathology*; D011509:Proteoglycans / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D011897:Random Allocation; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D000097608:UDP Xylose-Protein Xylosyltransferase
chemical_list: D010430:Pentosyltransferases; D011509:Proteoglycans; D000097608:UDP Xylose-Protein Xylosyltransferase