title: Induction of MiR-17-3p and MiR-106a [corrected] by TNFα and LPS.
abstract: MicroRNAs (miRNAs), small non-coding molecules, regulate gene expression in response to stimuli. Lipopolysaccharide (LPS) was reported to induce the expression of miR-146 and miR-155 in HL-60. The effects of LPS and the related stimulus, tumour necrosis factor alpha (TNFα), on miRNA expression required to be further studied. Using T7-oligo ligation assay (OLA)-based miRNA array, we profiled the expression of 132 miRNAs and identified a number of TNFα-regulated miRNAs in HeLa cells, including miR-17-3p and miR-106a. TNFα induction of miR-17-3p and miR-106a was verified by Northern blot analysis with RNU48 normalization. Northern blot analysis also showed that LPS was able to induce the expression of both miR-17-3p and miR-106a in HeLa cells. Furthermore, both array assay and Northern blot analysis showed that the expression levels of miR-146 and miR-155 were either low or undetectable in HeLa cells and TNFα- and LPS-mediated induction of these two miRNAs was not found. Luciferase reporter analysis confirmed the induction of miR-17-3p and miR-106a in response to TNFα and LPS treatment in HeLa cells. These results suggested that the expression of miR-17-3p and miR-106a is regulated by TNFα and LPS in HeLa cells.
authors.affiliation: Signosis, Inc., 528 Weddell Drive #5, Sunnyvale, CA 94089, USA. xinjiang@signosisinc.com
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Nancy
authors.initials: N
authors.lastname: Li
mesh_terms: D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; C542541:MIRN106 microRNA, human; C524941:MIRN17 microRNA, human; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha