title: Scanning the European corn borer (Ostrinia spp.) genome for adaptive divergence between host-affiliated sibling species.
abstract: It has recently been shown that the European corn borer, a major pest of maize crops, is actually composed of two genetically differentiated and reproductively isolated taxa, which are found in sympatry over a wide geographical range in Eurasia. Each taxon is adapted to specific host plants: Ostrinia nubilalis feeds mainly on maize, while O. scapulalis feeds mainly on hop or mugwort. Here, we present a genome scan approach as a first step towards an integrated molecular analysis of the adaptive genomic divergence between O. nubilalis and O. scapulalis. We analysed 609 AFLP marker loci in replicate samples of sympatric populations of Ostrinia spp. collected on maize, hop and mugwort, in France. Using two genome scan methods based on the analysis of population differentiation, we found a set of 28 outlier loci that departed from the neutral expectation in one or the other method (of which a subset of 14 loci were common to both methods), which showed a significantly increased differentiation between O. nubilalis and O. scapulalis, when compared to the rest of the genome. A subset of 12 outlier loci were sequenced, of which 7 were successfully re-amplified as target candidate loci. Three of these showed homology with annotated lepidopteran sequences from public nucleotide databases.
authors.affiliation: Institut National de la Recherche Agronomique, UMR CBGP (INRA-IRD-CIRAD-Montpellier SupAgro), Campus International de Baillarguet, CS 30016, Montferrier sur Lez Cedex, France.
authors.forename: Afiwa
authors.initials: A
authors.lastname: Midamegbe
authors.forename: Renaud
authors.initials: R
authors.lastname: Vitalis
authors.forename: Thibaut
authors.initials: T
authors.lastname: Malausa
authors.forename: Emilie
authors.initials: E
authors.lastname: Delava
authors.forename: Sandrine
authors.initials: S
authors.lastname: Cros-Arteil
authors.forename: Réjane
authors.initials: R
authors.lastname: Streiff
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D019306:Artemisia / Q000235:genetics / Q000469:parasitology; D016000:Cluster Analysis; D005819:Genetic Markers; D049750:Genome, Insect; D006790:Host-Parasite Interactions; D027582:Humulus / Q000235:genetics / Q000469:parasitology; D009036:Moths / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D003313:Zea mays / Q000235:genetics / Q000469:parasitology*
chemical_list: D005819:Genetic Markers