title: Primer extension.
abstract: Primer extension can be viewed as a technique complementary to S1 nuclease mapping (see Chapter 43). In Sl nuclease mapping analysis, a DNA:RNA hybrid is resected back to the point of divergence between the RNA and the DNA. In primer extension, a DNA probe is annealed to an RNA template and then extended in a 3';- 5'; direction to the start of the RNA molecule. The most common usages for this technique are in determining the size of full-length RNA transcripts and mapping transcription initiation sites.
authors.affiliation: Molecular Neurobiology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.
authors.forename: D R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D017931:DNA Primers / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005821:Genetic Techniques; D009693:Nucleic Acid Hybridization
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004262:DNA Restriction Enzymes