title: Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase from Arabidopsis thaliana.
abstract: 4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a=91.6, b=55.5, c=124.4 Å, α=γ=90.0, β=111.1°.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Takahiro
authors.initials: T
authors.lastname: Mori
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Wanibuchi
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kato
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Sugio
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; C058644:4-coumaroyl-coenzyme A; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase