title: Yeast transformation.
abstract: The yeast Saccharomyces cerevisiae has tremendous advantages as a host in gene cloning experiments. It is a microorganism for which most of the techniques developed in bacterial work can be applied, including chemical mutagenesis, selective plating, and replica plating. Being the basis of an ancient industry, its fermentation characteristics are well understood. It is also a eukaryote with nitotic and meiotic divisions, cellular compartmentalization, and post-translational modification of proteins similar to that seen in higher species. Yeast is thus an ideal organism to turn to in experiments for which bacterial hosts are not suitable, but microbial techniques are nonetheless required.
authors.affiliation: Division of Biological and Environmental Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, England.
authors.forename: E B
authors.initials: EB
authors.lastname: Gingold