title: A fungal nonribosomal peptide synthetase module that can synthesize thiopyrazines.
abstract: A nonribosomal peptide synthetase-like enzyme (NRPS325) from Aspergillus terreus was reconstituted in vitro and was shown to synthesize thiopyrazines using an unprecedented mechanism. Substrate promiscuity of NRPS325 toward different amino acids and free thiols was explored to produce >60 different thiopyrazine compounds.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California Los Angeles, Los Angeles, California 90095, United States.
authors.forename: Kangjian
authors.initials: K
authors.lastname: Qiao
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Neil
authors.initials: N
authors.lastname: Garg
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D015394:Molecular Structure; D005810:Multigene Family; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D011714:Pyrans / Q000138:chemical synthesis* / Q000378:metabolism; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis* / Q000378:metabolism
chemical_list: D011714:Pyrans; D013438:Sulfhydryl Compounds; C522033:thiopyran; D010453:Peptide Synthases