title: Viral cell-to-cell movement requires formation of cortical punctate structures containing Red clover necrotic mosaic virus movement protein.
abstract: Movement protein (MP) of Red clover necrotic mosaic virus (RCNMV) forms punctate structures on the cortical endoplasmic reticulum (ER) of Nicotiana benthamiana cells, which are associated with viral RNA1 replication (Kaido et al., Virology 395, 232-242. 2009). We investigated the significance of ER-targeting by MP during virus movement from cell to cell, by analyzing the function of a series of MPs with varying length deletions at their C-terminus, either fused or not fused with green fluorescent protein (GFP). The C-terminal 70 amino acids were crucial to ER-localization of MP-GFP and cell-to-cell movement of the recombinant virus encoding it. However, C-terminal deletion did not affect MP functions, such as increasing the size exclusion limit of plasmodesmata, single-stranded RNA binding in vitro, and MP interacting in vivo. We discuss the possible role of this MP region in virus movement from cell to cell.
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan. kaido@kais.kyoto-u.ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Kaido
authors.forename: Naoko
authors.initials: N
authors.lastname: Funatsu
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Tsuno
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Mise
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Okuno
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012329:RNA, Bacterial; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology; D019183:Tombusviridae / Q000378:metabolism*
chemical_list: D053830:Plant Viral Movement Proteins; C042027:RNA I; D012329:RNA, Bacterial; D012367:RNA, Viral