title: Construction and analysis of full-length cDNA library of Cryptosporidium parvum.
abstract: A full-length cDNA library was constructed from the sporozoite of Cryptosporidium parvum. Normalized clones were subjected to Solexa shotgun sequencing, and then complete sequences for 1066 clones were reconfigured. Detailed analyses of the sequences revealed that 13.5% of the transcripts were spliced; the average and median 5' UTR lengths were 213.5 and 122 nucleotides, respectively. There were 148 inconsistencies out of 562 examined genes between the experimentally described cDNA sequence and the predicted sequence from its genome. In addition, we identified 118 sequences that had little homology against annotated genes of C. parvum as prospective candidates for addable genes. These observations should improve the reliability of C. parvum transcriptome and provide a versatile resource for further studies.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Yamagishi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wakaguri
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Suguru
authors.initials: S
authors.lastname: Kawano
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sugimoto
authors.forename: Junichi
authors.initials: J
authors.lastname: Watanabe
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Isao
authors.initials: I
authors.lastname: Kimata
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D003001:Cloning, Molecular; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D017125:Genes, Protozoan; D018503:Genome, Protozoan; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D058977:Molecular Sequence Annotation; D009865:Oocytes / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D020121:5' Untranslated Regions; D018076:DNA, Complementary; D016053:RNA, Protozoan