title: Trypanosoma brucei brucei: A comparison of gene expression in the liver and spleen of infected mice utilizing cDNA microarray technology.
abstract: Trypanosoma brucei brucei, the infectious agent of the disease known as Nagana, is a pathogenic trypanosome occurring in Africa, where it causes significant economic loss to domesticated livestock. Although many studies on the histopathology of organs of mice infected with T. b. brucei have been reported, little work has been done regarding gene expression in these organs in infected mice. In this paper, we describe the use of cDNA microarray to determine gene expression profiles in the liver and spleen of mice infected with T. b. brucei (STIB 920) at peak parasitaemia (12 days after infection). Our results showed that a total of 123 genes in the liver and 389 genes in the spleen were expressed differentially in T. b. brucei infected mice. In contrast, however, in an acute infection in mice caused by Trypanosoma brucei evansi, a species genetically related to T. b. brucei, 336 genes in the liver and 190 genes in the spleen were expressed, differentially, indicating that the liver of mice was more affected by the acute T. b. evansi infection whilst the spleen was more affected by the subacute T. b. brucei infection. Our results provide a number of possible reasons why mice infected with T. b. evansi die sooner than those infected with T. b. brucei: (1) mice infected with T. b. evansi may need more stress response proteins to help them pass through the infection and these are probably excessively consumed; (2) proliferating cell nuclear antigen was more down-regulated in the liver of mice infected with T. b. evansi, which indicated that the inhibition of proliferation of hepatocytes in mice infected with T. b. evansi might be more severe than that in T. b. brucei infection; and (3) more hepatocyte apoptosis occurred in the mice infected with T. b. evansi and this might be probably the most important reason why mice died sooner than those infected with T. b. brucei. Studies of the changes in the gene expression profile in the liver and spleen of mice infected with T. b. brucei may be helpful in understanding the mechanisms of pathogenesis in Nagana disease at the molecular level. By comparing the gene profiles of the liver and spleen of mice infected with T. b. brucei with T. b. evansi, we have identified a number of factors that could explain the differences in pathogenesis in mice infected with these two African trypanosomes.
authors.affiliation: Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Key Laboratory of Tropical Diseases Control, The Ministry of Education, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, PR China.
authors.forename: San-Qiang
authors.initials: SQ
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Luckins
authors.forename: Zhao-Rong
authors.initials: ZR
authors.lastname: Lun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015870:Gene Expression / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D013154:Spleen / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D014346:Trypanosoma brucei brucei / Q000502:physiology*; D014353:Trypanosomiasis, African / Q000209:etiology / Q000469:parasitology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018809:Proliferating Cell Nuclear Antigen