title: Genital tract sequestration of SIV following acute infection.
abstract: We characterized the evolution of simian immunodeficiency virus (SIV) in the male genital tract by examining blood- and semen-associated virus from experimentally and sham vaccinated rhesus monkeys during primary infection. At the time of peak virus replication, SIV sequences were intermixed between the blood and semen supporting a scenario of high-level virus "spillover" into the male genital tract. However, at the time of virus set point, compartmentalization was apparent in 4 of 7 evaluated monkeys, likely as a consequence of restricted virus gene flow between anatomic compartments after the resolution of primary viremia. These findings suggest that SIV replication in the male genital tract evolves to compartmentalization after peak viremia resolves.
authors.affiliation: Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA. jwhitne2@bidmc.harvard.edu
authors.forename: James B
authors.initials: JB
authors.lastname: Whitney
authors.forename: Peter T
authors.initials: PT
authors.lastname: Hraber
authors.forename: Corinne
authors.initials: C
authors.lastname: Luedemann
authors.forename: Elena E
authors.initials: EE
authors.lastname: Giorgi
authors.forename: Marcus G
authors.initials: MG
authors.lastname: Daniels
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Bhattacharya
authors.forename: Srinivas S
authors.initials: SS
authors.lastname: Rao
authors.forename: John R
authors.initials: JR
authors.lastname: Mascola
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Nabel
authors.forename: Bette T
authors.initials: BT
authors.lastname: Korber
authors.forename: Norman L
authors.initials: NL
authors.lastname: Letvin
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000235:genetics; D051456:Gene Flow; D015686:Gene Products, env / Q000235:genetics*; D005837:Genitalia, Male / Q000821:virology*; D008253:Macaca mulatta; D008297:Male; D016133:Polymerase Chain Reaction; D012661:Semen / Q000821:virology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000517:prevention & control* / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000472:pathogenicity*; D014611:Vaccination; D019562:Viral Load; D014766:Viremia / Q000517:prevention & control; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D015686:Gene Products, env