title: Laryngeal regeneration using tissue engineering techniques in a canine model.
abstract: OBJECTIVE
  We previously reported that polypropylene mesh covered with collagen sponge is a useful material for the regeneration of the trachea and the cricoid cartilage. The aim of this study was to regenerate larynges after partial hemilaryngectomy with this new biomaterial.
  
  
  METHODS
  A left partial hemilaryngectomy was performed on 12 adult beagles. The defect size was about 1.8 x 1.0 cm. Both sides of polypropylene mesh were coated with either 1% or 3% collagen sponge. This scaffold was wrapped in fascia lata harvested from the left thigh and then fixed in place over the defect. Endoscopic examinations were performed periodically. Six months after treatment, 3-dimensional computed tomographic scanning was performed. Vibratory examinations were also performed with excised larynges.
  
  
  RESULTS
  In the 1% collagen group, exposure or dislocation of the mesh was found in 3 of 6 cases, but in the 3% group, no exposure of the mesh was seen. The morphological findings in the vocal fold were better in the 3% group than in the 1% group, but a difference in the vertical levels of the vocal folds was found in both groups.
  
  
  CONCLUSIONS
  This study suggests that 3% collagen-coated polypropylene mesh wrapped with autologous fascia is a useful material for laryngeal regeneration.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Kitani
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kanemaru
authors.forename: Hiroo
authors.initials: H
authors.lastname: Umeda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suehiro
authors.forename: Yo
authors.initials: Y
authors.lastname: Kishimoto
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000818:Animals; D003094:Collagen; D004285:Dogs; D005206:Fascia Lata; D007830:Larynx / Q000502:physiology*; D011126:Polypropylenes; D012038:Regeneration / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014827:Vocal Cords
chemical_list: D011126:Polypropylenes; D003094:Collagen