title: Halomonas xianhensis sp. nov., a moderately halophilic bacterium isolated from a saline soil contaminated with crude oil.
abstract: A Gram-negative, aerobic, short rod-shaped and non-motile bacterium, strain A-1(T), was isolated from a saline soil contaminated with crude oil in Xianhe, Shangdong Province, China. Strain A-1(T) formed yellow colonies, was moderately halophilic and grew with 0.05-27.5% (w/v) total salts (optimum 5-8%), at 10-42 °C (optimum 30 °C) and at pH 5.5-9.0 (optimum pH 7.2). The dominant fatty acids (>5%) were C(16:0), summed feature 3 (comprising C(16:1)ω7c and/or iso-C(15:0) 2-OH), C(18:1)ω7c, C(19:0) cyclo ω8c and C(12:0) 3-OH and the predominant ubiquinone was Q-9. The genomic DNA G+C content was 67.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A-1(T) belonged to the genus Halomonas in the class Gammaproteobacteria. The closest relatives were Halomonas lutea YIM 91125(T) (97.7% 16S rRNA gene sequence similarity), H. muralis LMG 20969(T) (95.6%), H. pantelleriensis AAP(T) (95.5%) and H. kribbensis BH843(T) (95.2%). DNA-DNA relatedness between strain A-1(T) and H. lutea CCTCC AB 206093(T) was 27±3%. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain A-1(T) should be placed in the genus Halomonas as a representative of a novel species. The name Halomonas xianhensis sp. nov. is proposed, with strain A-1(T) (=CGMCC 1.6848(T) =JCM 14849(T)) as the type strain.
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Baisuo
authors.initials: B
authors.lastname: Zhao
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Mao
authors.affiliation: State Key Joint Laboratory on Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Li
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yun-Jiao
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Shukun
authors.initials: S
authors.lastname: Tang
authors.affiliation: The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010578:Petroleum; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010578:Petroleum; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012492:Salts; D012989:Soil Pollutants