title: Structural and biological characterization of Nattectin, a new C-type lectin from the venomous fish Thalassophryne nattereri.
abstract: Lectins are glycan-binding receptors that recognize glycan epitopes on foreign pathogens and in the host systems. They can be involved in functions that include innate immunity, development, immune regulation and homeostasis. Several lectins have been purified and characterized from fish species. In this work, using cation-exchange chromatography, a galactose-specific lectin belonging to the family of C-type lectins was isolated from the venom of the Brazilian venomous fish Thalassophryne nattereri. Nattectin is a basic, non-glycosilated, 15 kDa monomeric protein. It exhibits hemagglutination activity that is independent of Ca(2+). We also demonstrated a lectin activity for Nattectin in the innate immune system, especially in neutrophil mobilization in mice, indicating that marine organisms are source of immunomodulator agents.
authors.affiliation: Laboratório Especial de Toxinologia Aplicada (CEPID/FAPESP), Instituto Butantan, Avenida Vital Brazil, 1500. Butantan. São Paulo, São Paulo. Brazil. lopesferreira@butantan.gov.br
authors.forename: Mônica
authors.initials: M
authors.lastname: Lopes-Ferreira
authors.forename: Geraldo Santana
authors.initials: GS
authors.lastname: Magalhães
authors.forename: Jorge Hernandez
authors.initials: JH
authors.lastname: Fernandez
authors.forename: Inácio de Loiola M
authors.initials: Ide L
authors.lastname: Junqueira-de-Azevedo
authors.forename: Paulo
authors.initials: P
authors.lastname: Le Ho
authors.forename: Carla
authors.initials: C
authors.lastname: Lima
authors.forename: Richard H
authors.initials: RH
authors.lastname: Valente
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Moura-da-Silva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D024541:Batrachoidiformes; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement; D017124:Conserved Sequence; D005397:Fish Venoms / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D006386:Hemagglutination Tests; D006614:Hindlimb / Q000473:pathology; D006801:Humans; D007113:Immunity, Innate; D007155:Immunologic Factors / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology; D037181:Lectins, C-Type / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein; D040681:Structural Homology, Protein
chemical_list: D005397:Fish Venoms; D007155:Immunologic Factors; D037181:Lectins, C-Type; C581368:nattectin protein, Thalassophryne nattereri; D020782:Matrix Metalloproteinases; D002118:Calcium; D005690:Galactose