title: Albumin adsorption on unmodified and sulfonated polystyrene surfaces, in relation to cell-substratum adhesion.
abstract: Albumin is commonly applied for blocking the adsorption of other proteins and to prevent the nonspecific adhesion of cells to diverse artificial substrata. Here we address the question of how effective these albumin properties are--by investigating unmodified and sulfonated polystyrene substrata with distinctly different wettabilities. As clearly shown with (125)I-radioisotopic assays, above a concentration of 10-20 μg/mL, the efficiency of bovine serum albumin (BSA) adsorption became markedly higher on the sulfonated surface than on the unmodified one. This study was assisted with the atomic force microscopy. On the unmodified surface, BSA, adsorbed from sufficiently concentrated solutions, formed a monolayer, with occasional intrusions of multilayered patches. Conversely, the arrangement of BSA on the sulfonated surface was chaotic; the height of individual molecules was lower than on the unmodified polystyrene. Importantly, the adhesion study of LNCaP and DU145 cells indicated that both surfaces, subjected to the prior BSA adsorption, did not completely loose their cell-adhesive properties. However, the level of adhesion and the pattern of F-actin organization in adhering cells have shown that cells interacted with unmodified and sulfonated surfaces differently, depending on the arrangement of adsorbed albumin. These results suggest the presence of some bare substratum area accessible for cells after the albumin adsorption to both types of investigated surfaces.
authors.affiliation: Department of Biophysics, Medical Centre for Postgraduate Education, ul. Marymoncka 99, 01-813 Warszawa, Poland. hmkowal@gmail.com
authors.forename: Hanna M
authors.initials: HM
authors.lastname: Kowalczyńska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Nowak-Wyrzykowska
authors.forename: Andrzej A
authors.initials: AA
authors.lastname: Szczepankiewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Dobkowski
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dyda
authors.forename: Jarosław
authors.initials: J
authors.lastname: Kamiński
authors.forename: Robert
authors.initials: R
authors.lastname: Kołos
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D018625:Microscopy, Atomic Force; D011137:Polystyrenes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011137:Polystyrenes; D012710:Serum Albumin, Bovine; C003321:polystyrene sulfonic acid