title: Site-selective binding of nanoparticles to double-stranded DNA via peptide nucleic acid "invasion".
abstract: We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Stadler
authors.forename: Dazhi
authors.initials: D
authors.lastname: Sun
authors.forename: Mathew M
authors.initials: MM
authors.lastname: Maye
authors.forename: Daniel
authors.initials: D
authors.lastname: van der Lelie
authors.forename: Oleg
authors.initials: O
authors.lastname: Gang
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017931:DNA Primers; D020135:Peptide Nucleic Acids; D004247:DNA