title: A novel alkaline lipase from Ralstonia with potential application in biodiesel production.
abstract: With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000 Da by SDS-PAGE. The activity was highest at 50-55°C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45°C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and K(m) and V(max) for p-nitrophenyl palmitate were 2.73 ± 0.6mM and 101.4 ± 1.9 mM/min mg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Hah-Young
authors.initials: HY
authors.lastname: Yoo
authors.forename: Jaya Ram
authors.initials: JR
authors.lastname: Simkhada
authors.forename: Seung Sik
authors.initials: SS
authors.lastname: Cho
authors.forename: Don Hee
authors.initials: DH
authors.lastname: Park
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Chi Nam
authors.initials: CN
authors.lastname: Seong
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D000595:Amino Acid Sequence; D056804:Biofuels; D004591:Electrophoresis, Polyacrylamide Gel; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D043367:Ralstonia / Q000145:classification / Q000201:enzymology*; D013696:Temperature
chemical_list: D056804:Biofuels; D008049:Lipase