title: Biomechanical and histologic evaluation of tissue engineered ligaments using chitosan and hyaluronan hybrid polymer fibers: a rabbit medial collateral ligament reconstruction model.
abstract: In this study, we used a rabbit medial collateral ligament reconstruction model to evaluate a novel chitosan-based hyaluronan hybrid polymer fiber scaffold for ligament tissue engineering and to examine whether mechanical forces exerted in an in vivo model increased extracellular matrix production by seeded fibroblasts. Scaffolds were used 2 weeks after incubation with fibroblasts obtained from the same rabbit in a cell-seeded scaffold (CSS) group and without cells in a noncell-seeded scaffold (NCSS) group. At 3, 6, and 12 weeks after surgery, the failure loads of the engineered ligaments in the CSS groups were significantly greater than those in the NCSS groups. At 6 weeks after surgery, the reconstructed tissue of the CSS group was positive for type I collagen, whereas that in the NCSS group was negative for type I collagen. At 12 weeks after surgery, the reconstructed tissue stained positive for type I collagen in the CSS group, but negative in the NCSS group. Our results indicate that the scaffold material enhanced the production of type I collagen and led to improved mechanical strength in the engineered ligament in vivo.
authors.affiliation: Department of Orthopaedic Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Irie
authors.forename: Tokifumi
authors.initials: T
authors.lastname: Majima
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sawaguchi
authors.forename: Tadanao
authors.initials: T
authors.lastname: Funakoshi
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
authors.forename: Akio
authors.initials: A
authors.lastname: Minami
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D024042:Collagen Type I / Q000737:chemistry; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry*; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry / Q000379:methods; D008022:Ligaments / Q000378:metabolism*; D017888:Medial Collateral Ligament, Knee / Q000473:pathology*; D023421:Models, Animal; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D024042:Collagen Type I; D011108:Polymers; D006820:Hyaluronic Acid; D048271:Chitosan