title: Characterization of a salt-tolerant xylanase from Thermoanaerobacterium saccharolyticum NTOU1.
abstract: A xylanase gene was PCR-cloned from Thermoanaerobacterium saccharolyticum and expressed in Escherichia coli. The xylanase (XynA) consisted of a signal peptide, glycoside hydrolase family 10 domains, carbohydrate-binding modules, and surface layer homology domains. It was optimally active at 70-73°C and at pH 5-7. It had enhanced activity with NaCl with optimal activity at 0.4 M but was tolerant up to 2 M NaCl. The thermostable and salt-tolerant properties of this xylanase suggest that it may be useful for industrial applications.
authors.affiliation: Institute of Bioscience and Biotechnology, National Taiwan Ocean University, No. 2 Pei-Ning Road, Keelung, Taiwan, People's Republic of China.
authors.forename: Kuo-Sheng
authors.initials: KS
authors.lastname: Hung
authors.forename: Shiu-Mei
authors.initials: SM
authors.lastname: Liu
authors.forename: Tsuei-Yun
authors.initials: TY
authors.lastname: Fang
authors.forename: Wen-Shyong
authors.initials: WS
authors.lastname: Tzou
authors.forename: Fu-Pang
authors.initials: FP
authors.lastname: Lin
authors.forename: Kuang-Hui
authors.initials: KH
authors.lastname: Sun
authors.forename: Shye-Jye
authors.initials: SJ
authors.lastname: Tang
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020536:Enzyme Activators / Q000378:metabolism; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D017434:Protein Structure, Tertiary; D012492:Salts / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013696:Temperature; D046950:Thermoanaerobacterium / Q000201:enzymology*; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D020536:Enzyme Activators; D021382:Protein Sorting Signals; D012492:Salts; D014995:Xylosidases