title: Determination of fluoroacetate and fluoride in blood serum by capillary zone electrophoresis using capacitively coupled contactless conductivity detection.
abstract: Fluoroacetate is a highly toxic species naturally found in plants and in commercial products (compound 1080) for population control of several undesirable animal species. However, it is non-selective and toxic to many other animals including humans, and thus its detection is very important for forensic purposes. This paper presents a sensitive and fast method for the determination of fluoroacetate in blood serum using capillary electrophoresis with capacitively coupled contactless conductivity detection. Serum blood samples were treated with ethanol to remove proteins. The samples were analyzed in BGE containing 15 mmol/L histidine and 30 mmol/L gluconic acid (pH 3.85). The calibration curve was linear up to 75 μmol/L (R² =0.9995 for N=12). The detection limit in the blood serum was 0.15 mg/kg, which is smaller than the lethal dose for humans and other animals. Fluoride, a metabolite of the fluoroacetate defluorination, could also be detected for levels greater than 20 μmol/L, when polybrene was used for reversion of the EOF. CTAB and didecyldimethylammonium bromide are not useful for this task because of the severe reduction of the fluoride level. However, no interference was observed for fluoroacetate.
authors.affiliation: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Denis Tadeu Rajh
authors.initials: DT
authors.lastname: Vidal
authors.forename: Marcio Antonio
authors.initials: MA
authors.lastname: Augelli
authors.forename: Guilherme Minoru
authors.initials: GM
authors.lastname: Hotta
authors.forename: Fernando Silva
authors.initials: FS
authors.lastname: Lopes
authors.forename: Claudimir Lucio
authors.initials: CL
authors.lastname: do Lago
mesh_terms: D000818:Animals; D002417:Cattle; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D005459:Fluorides / Q000097:blood*; D005463:Fluoroacetates / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D005463:Fluoroacetates; C014815:fluoroacetic acid; D005459:Fluorides