title: Superior mineralization and neovascularization capacity of adult human metaphyseal periosteum-derived cells for skeletal tissue engineering applications.
abstract: Bone tissue engineering is a promising cell-based strategy to treat bone defects. Mesenchymal stem cells from adult human bone marrow (hBMSCs) are a frequently used cellular source for bone tissue generation. However, the low frequency of these stem cells in adult bone marrow and their limited proliferation restrict their clinical utility. An alternative source of MSCs is the periosteum-derived cells, and these cells appear to be easy to harvest and expand ex vivo. We isolated human metaphyseal periosteum-derived cells (hMPCs) and hBMSCs from the same donors and compared their osteogenic capacity both in vitro and in vivo. After osteogenic induction in monolayer cultures, hMPCs resulted in more robust mineralization and expressed higher mRNA levels of BMP-2, osteopontin and osteocalcin than hBMSCs. Eight weeks after implantation of cellular-β-TCP scaffolds in immunodeficient mice, hMPC implantation showed higher neovascularization and higher percentage of mature bone formation than hBMSC implantation. In conclusion, hMPCs represent a promising cellular candidate for bone tissue engineering.
authors.affiliation: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, PR China.
authors.forename: Daoyun
authors.initials: D
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.forename: Junjie
authors.initials: J
authors.lastname: Shao
authors.forename: Yao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jianxi
authors.initials: J
authors.lastname: Lu
authors.forename: Yaohua
authors.initials: Y
authors.lastname: He
authors.forename: Chenglong
authors.initials: C
authors.lastname: Huang
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics; D001861:Bone Regeneration; D001842:Bone and Bones / Q000098:blood supply* / Q000502:physiology; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000627:therapeutic use; D002454:Cell Differentiation; D017690:Cell Transplantation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D015675:Osteocalcin / Q000235:genetics; D053495:Osteopontin / Q000235:genetics; D010521:Periosteum / Q000166:cytology*; D023822:Tissue Engineering; D014158:Transcription, Genetic; D055815:Young Adult
chemical_list: D000943:Antigens, Differentiation; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D053495:Osteopontin; D000469:Alkaline Phosphatase