title: Microarray-based CGH and copy number analysis of FFPE samples.
abstract: Over the past decade, utilization of microarray technology has flourished in biomedical research. It has evolved rapidly into a revolutionary tool that offers deeper insight into the molecular basis associated with complex diseases, especially in the field of cancer. Specifically, array-based Comparative Genomic Hybridization (aCGH) permits the detection of genome-wide copy number alterations with high resolution. Microarray application to DNA extracted from formalin-fixed paraffin-embedded tissue (FFPE), in particular, poses a challenge due to the partially degraded nature and compromised quality of the DNA. This chapter gives a description of the several CGH-microarray platforms currently available and offers practical steps that guide you through optimal handling and superior aCGH data acquisition of DNA extracted from FFPE tissues.
authors.affiliation: Molecular Pathology Unit, Health Sciences Center, Safat, Kuwait. fahd@al-mulla.org
authors.forename: Fahd
authors.initials: F
authors.lastname: Al-Mulla
mesh_terms: D002458:Cell Fractionation; D002675:Child, Preschool; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D004396:Coloring Agents; D055028:Comparative Genomic Hybridization / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D053938:DNA Fragmentation; D005557:Formaldehyde / Q000737:chemistry*; D018628:Gene Dosage / Q000235:genetics*; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods*; D013194:Staining and Labeling; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D004396:Coloring Agents; D005557:Formaldehyde; D004247:DNA