title: In-vivo-induced antigenic determinants of Fusobacterium nucleatum subsp. nucleatum.
abstract: Fusobacterium nucleatum plays a pivotal role in dental plaque biofilm formation and is known to be involved in chronic inflammatory systemic disease. However, limited knowledge of F. nucleatum genes expressed in vivo interferes with our understanding of pathogenesis. In this study, we identified F. nucleatum genes induced in vivo using in-vivo-induced antigen technology (IVIAT). Among 30,000 recombinant clones screened, 87 reacted reproducibly with pooled sera from 10 patients with periodontitis. The clones encoded for 32 different proteins, of which 28 could be assigned to their functions, which were categorized in translation, transcription, transport, energy metabolism, cell envelope, cellular process, fatty acid and phospholipid metabolism, transposition, cofactor biosynthesis, amino acid biosynthesis, and DNA replication. Putative virulence factors detected were ABC transporter, butyrate-acetoacetate CoA-transferase, hemin receptor, hemolysin, hemolysin-related protein, LysR family transcriptional regulator, serine protease, and transposase. Analysis of immune responses to the in-vivo-induced (ivi) antigens in five patients demonstrated that most were reactive to these proteins, confirming results with pooled sera. IVIAT-identified F. nucleatum genes in this study may accelerate the elucidation of F. nucleatum-mediated molecular pathogenesis.
authors.affiliation: Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Korea.
authors.forename: H-R
authors.initials: HR
authors.lastname: Lee
authors.forename: I-C
authors.initials: IC
authors.lastname: Rhyu
authors.forename: H-D
authors.initials: HD
authors.lastname: Kim
authors.forename: H-K
authors.initials: HK
authors.lastname: Jun
authors.forename: B-M
authors.initials: BM
authors.lastname: Min
authors.forename: S-H
authors.initials: SH
authors.lastname: Lee
authors.forename: B-K
authors.initials: BK
authors.lastname: Choi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D001692:Biological Transport / Q000235:genetics; D002460:Cell Line; D002473:Cell Wall / Q000648:ultrastructure; D015256:Coenzyme A-Transferases / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D000939:Epitopes / Q000276:immunology; D005674:Fusobacterium Infections / Q000276:immunology*; D016967:Fusobacterium nucleatum / Q000145:classification / Q000235:genetics* / Q000276:immunology; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D010518:Periodontitis / Q000097:blood / Q000382:microbiology; D014176:Protein Biosynthesis / Q000235:genetics; D057057:Serine Proteases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D019895:Transposases / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D006460:Hemolysin Proteins; D014157:Transcription Factors; D037521:Virulence Factors; C073470:LysR protein, Bacteria; D019895:Transposases; D015256:Coenzyme A-Transferases; D057057:Serine Proteases