title: Anti-inflammatory diterpene from Thyrsanthera suborbicularis.
abstract: Bioactivity-guided isolation on a n-hexane-soluble fraction of Thyrsanthera suborbicularis led to the isolation of a new rosane-type diterpene, 19-hydroxy-1(10), 15-rosadiene (1), along with three known compounds, taraxerol, acetyl aleuritolic acid, and spathulenol. The structures of isolated compounds were determined by interpretation of NMR spectroscopic data and mass spectrometry. Compound 1 demonstrated significantly inhibitory activity on nitric oxide production in RAW264.7 lipopolysaccharide (LPS)-activated mouse macrophages with an IC(50) value of 2.91 µg/ml via the suppression of inducible nitric oxide synthase (iNOS) mRNA expression.
authors.affiliation: University of Science & Technology, Daejeon, Korea.
authors.forename: Piseth
authors.initials: P
authors.lastname: Khiev
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Chae
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.forename: Kyung-Seop
authors.initials: KS
authors.lastname: Ahn
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification; D019665:Euphorbiaceae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II