title: Beyond reduction of atherosclerosis: PON2 provides apoptosis resistance and stabilizes tumor cells.
abstract: Major contributors to atherosclerosis are oxidative damage and endoplasmic reticulum (ER) stress-induced apoptosis; both of which can be diminished by the anti-oxidative protein paraoxonase-2 (PON2). ER stress is also relevant to cancer and associated with anti-cancer treatment resistance. Hence, we addressed, for the first time, whether PON2 contributes to tumorigenesis and apoptotic escape. Intriguingly, we found that several human tumors upregulated PON2 and such overexpression provided resistance to different chemotherapeutics (imatinib, doxorubicine, staurosporine, or actinomycin) in cell culture models. This was reversed after PON2 knock-down. Remarkably, just deficiency of PON2 caused apoptosis of selective tumor cells per se, demonstrating a previously unanticipated oncogenic function. We found a dual mechanistic role. During ER stress, high PON2 levels lowered redox-triggered induction of pro-apoptotic CHOP particularly via the JNK pathway, which prevented mitochondrial cell death signaling. Apart from CHOP, PON2 also diminished intrinsic apoptosis as it prevented mitochondrial superoxide formation, cardiolipin peroxidation, cytochrome c release, and caspase activation. Ligand-stimulated apoptosis by TRAIL or TNFα remained unchanged. Finally, PON2 knock-down caused vast reactive oxygen species formation and stimulated JNK-triggered CHOP expression, but inhibition of JNK signaling did not prevent cell death, demonstrating the pleiotropic, dominating anti-oxidative effect of PON2. Therefore, targeting redox balance is powerful to induce selective tumor cell death and proposes PON2 as new putative anti-tumor candidate.
authors.affiliation: Institute of Pharmacology, University Medical Center, Obere Zahlbacher Street 67, Mainz, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Witte
authors.forename: S
authors.initials: S
authors.lastname: Altenhöfer
authors.forename: P
authors.initials: P
authors.lastname: Wilgenbus
authors.forename: J
authors.initials: J
authors.lastname: Amort
authors.forename: A M
authors.initials: AM
authors.lastname: Clement
authors.forename: A
authors.initials: A
authors.lastname: Pautz
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: U
authors.initials: U
authors.lastname: Förstermann
authors.forename: S
authors.initials: S
authors.lastname: Horke
mesh_terms: D017209:Apoptosis; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D050197:Atherosclerosis / Q000378:metabolism* / Q000503:physiopathology; D045744:Cell Line, Tumor; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D043303:Aryldialkylphosphatase; C468411:PON2 protein, human