title: In vitro inhibitory effects of cyandin-3-rutinoside on pancreatic α-amylase and its combined effect with acarbose.
abstract: The inhibitory activity on pancreatic α-amylase by cyanidin-3-rutinoside was examined in vitro. The IC₅₀ value of cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Sarinya
authors.initials: S
authors.lastname: Akkarachiyasit
authors.forename: Sirintorn
authors.initials: S
authors.lastname: Yibchok-Anun
authors.forename: Sumrit
authors.initials: S
authors.lastname: Wacharasindhu
authors.forename: Sirichai
authors.initials: S
authors.lastname: Adisakwattana
mesh_terms: D020909:Acarbose / Q000494:pharmacology*; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D004357:Drug Synergism; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D007700:Kinetics; D015394:Molecular Structure; D010179:Pancreas / Q000201:enzymology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000516:alpha-Amylases; C428983:cyanidin 3-rutinoside; D020909:Acarbose