title: Urinary transforming growth factor-β1 in children with obstructive uropathy.
abstract: OBJECTIVE
  Obstructive uropathies (OU) in childhood constitute one of the major causes of chronic renal insufficiency. Transforming growth factor-β1 (TGF-β1) is considered to be the major fibrogenic growth factor. The aim of the present study was to investigate urinary TGF-β1 levels in children with obstructive and non-obstructive uropathies (NOU).
  
  
  METHODS
  This study involved 19 children with OU, 11 children with non-obstructive hydronephrosis and 21 healthy children. Urinary TGF-β1, proteinuria, microalbuminuria and urinary α1-microglobulin were measured, and renal function was assessed. The results were statistically analyzed.
  
  
  RESULTS
  Mean urinary TGF-β1 concentrations in patients with OU were significantly higher than those with NOU (4.14 ± 0.67 creatinine vs 1.80 ± 0.24 pg/mmol creatinine, P < 0.05) and healthy controls (1.66 ± 0.28 pg/mmol creatinine, P < 0.05). Positive correlations of urinary TGF-β1 concentrations with proteinuria (r = 0.87, P < 0.0001) and urinary α1-microglobulin (r = 0.82, P = 0.0002) were found in patients with OU.
  
  
  CONCLUSIONS
  Children with OU have higher urinary TGF-β1 than children with NOU. Urinary TGF-β1 may be a useful non-invasive tool for the differential diagnosis between OU and NOU in children. A positive correlation of TGF-β1 with markers of renal tissue damage in patients with OU was found.
authors.affiliation: Department of Paediatrics, Second Medical School, Charles University Prague, University Hospital Motol, Prague, Czech Republic. jakubzieg@hotmail.com
authors.forename: Jakub
authors.initials: J
authors.lastname: Zieg
authors.forename: Kveta
authors.initials: K
authors.lastname: Blahova
authors.forename: Tomas
authors.initials: T
authors.lastname: Seeman
authors.forename: Jiri
authors.initials: J
authors.lastname: Bronsky
authors.forename: Hana
authors.initials: H
authors.lastname: Dvorakova
authors.forename: Marta
authors.initials: M
authors.lastname: Pechova
authors.forename: Jan
authors.initials: J
authors.lastname: Janda
authors.forename: Karel
authors.initials: K
authors.lastname: Matousovic
mesh_terms: D000419:Albuminuria / Q000209:etiology / Q000652:urine; D000510:Alpha-Globulins / Q000652:urine; D000704:Analysis of Variance; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine; D003430:Cross-Sectional Studies; D018153:Czech Republic; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D006869:Hydronephrosis / Q000209:etiology / Q000652:urine; D007223:Infant; D007668:Kidney / Q000503:physiopathology; D008297:Male; D011237:Predictive Value of Tests; D051436:Renal Insufficiency, Chronic / Q000175:diagnosis / Q000209:etiology* / Q000503:physiopathology / Q000652:urine; D053773:Transforming Growth Factor beta1 / Q000652:urine*; D015854:Up-Regulation; D014517:Ureteral Obstruction / Q000150:complications / Q000175:diagnosis / Q000503:physiopathology / Q000652:urine*; D014524:Urethral Obstruction / Q000150:complications / Q000175:diagnosis / Q000503:physiopathology / Q000652:urine*
chemical_list: D000510:Alpha-Globulins; D015415:Biomarkers; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; C016001:alpha-1-microglobulin; D003404:Creatinine