title: Submicron polymer particles containing fluorescent semiconductor nanocrystals CdSe/ZnS for bioassays.
abstract: OBJECTIVE
  This study aimed to design a panel of uniform particulate biochemical reagents and to test them in specific bioassays. These reagents are polymer particles of different sizes doped with semiconductor nanocrystals and conjugated with either full-size antibodies or recombinant mini-antibodies (4D5 scFv fragment) designed by genetic engineering approaches.
  
  
  METHODS
  A panel of highly fluorescent polymer particles (150-800 nm) were formed by embedding CdSe/ZnS nanocrystals (quantum dots) into preformed polyacrolein and poly(acrolein-co-styrene) particles. Morphology, content and fluorescence characteristics of the prepared materials were studied by laser correlation spectroscopy, spectrophotometry, optical and fluorescent microscopy and fluorimetry.
  
  
  RESULTS
  The obtained fluorescent particles sensitized by anti-Yersinia pestis antibodies were used for rapid agglutination glass test suitable for screening analysis of Y. pestis antigen and for microtiter particle agglutination, which, owing to its speed and simplicity, is very beneficial for diagnostic detection of Y. pestis antigen. Recombinant 4D5 scFv antibodies designed and conjugated with polymer particles containing quantum dots provide multipoint highly specific binding with cancer marker HER2/neu on the surface of SKOV-3 cell.
authors.affiliation: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117871, Russia. a-generalova@yandex.ru
authors.forename: Alla N
authors.initials: AN
authors.lastname: Generalova
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Sizova
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Zdobnova
authors.forename: Margarita M
authors.initials: MM
authors.lastname: Zarifullina
authors.forename: Michail V
authors.initials: MV
authors.lastname: Artemyev
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Baranov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Oleinikov
authors.forename: Vitaly P
authors.initials: VP
authors.lastname: Zubov
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Deyev
mesh_terms: D000171:Acrolein / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D015200:Fluoroimmunoassay / Q000379:methods; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D010930:Plague / Q000175:diagnosis*; D011108:Polymers / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry*; D012666:Semiconductors; D013440:Sulfides / Q000737:chemistry*; D015010:Yersinia pestis / Q000276:immunology / Q000302:isolation & purification*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D011108:Polymers; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C029975:polyacrolein; D000171:Acrolein; C058667:cadmium selenide; C031238:zinc sulfide