title: Rapid quantification of quinine and its major metabolite (3S)-3-hydroxyquinine in diluted urine by UPLC-MS/MS.
abstract: A rapid UPLC-MS/MS quantitative assay for the quantification of quinine and (3S)-3-hydroxyquinine requiring minimal sample pre-treatment - dilute-and-shoot type approach - has been developed. The assay was run at 0.6mL/min using gradient elution with (pH 10; 10mM) ammonium bicarbonate and methanol with a total cycle time of 2.5min on a 50mm×2.1mm ID, 1.7μm Acquity BEH column. Peak shapes were highly symmetrical allowing for accurate peak integration. Calibration curves for both analytes were constructed from 1.00 to 20.00ng/mL, yielding R(2) values >0.995. Intra- and inter-batch assay precision and accuracy were evaluated using 6 injections of QC solutions on 3 separate days (n=18) and were found to be within ±10% and 90-110% respectively. The method was shown to be suitable for quantitatively determining the ratio of quinine to (3S)-3-hydroxyquinine for a cohort of samples from an epidemiological study.
authors.affiliation: Pharmaceutical Science Division, School of Biomedical and Health Sciences, King's College London, United Kingdom. james.heaton@kcl.ac.uk
authors.forename: James
authors.initials: J
authors.lastname: Heaton
authors.forename: Nilufer
authors.initials: N
authors.lastname: Rahmioglu
authors.forename: Kourosh R
authors.initials: KR
authors.lastname: Ahmadi
authors.forename: Cristina
authors.initials: C
authors.lastname: Legido-Quigley
authors.forename: Norman W
authors.initials: NW
authors.lastname: Smith
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D011802:Quinidine / Q000031:analogs & derivatives* / Q000652:urine; D011803:Quinine / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D012996:Solutions; D053719:Tandem Mass Spectrometry
chemical_list: D012996:Solutions; C013569:3-hydroxyquinidine; D011803:Quinine; D011802:Quinidine