title: HMGN5: a potential oncogene in gliomas.
abstract: Gliomas are the most common primary brain tumors in the central nervous system and a leading cause of tumor-related death. High-mobility group nucleosome binding domain 5 (HMGN5/NSBP1), which is highly expressed in breast cancer and in hormone-induced mouse uterine adenocarcinoma, acts as a potential oncogene in gliomas. In this study, the role of HMGN5 in the proliferation of human glioma cells was investigated by lentivirus-mediated RNA interference (RNAi). The decrease in HMGN5 expression in human glioma U251 and U87 cells caused cell cycle arrest in the G1 phase and a delay in cell proliferation, as well as resulting in more apoptosis and an inhibition of clonogenic growth in soft agar in U251 cells; these results suggest that HMGN5 is required for tumorigenesis in vitro. Furthermore, HMGN5 was highly expressed in both high-grade and low-grade glioma tissue samples compared with normal brain tissues. Collectively, our data suggest that HMGN5 may play a critical role in the development of gliomas.
authors.affiliation: Department of Neurosurgery, Shanghai Institute of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Qu
authors.forename: Runmin
authors.initials: R
authors.lastname: Yan
authors.forename: Juxiang
authors.initials: J
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Jingxu
authors.initials: J
authors.lastname: Zhou
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Yan
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism*; D024001:HMGN Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D039423:Integrin alpha1 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014162:Transfection / Q000379:methods; D055815:Young Adult
chemical_list: D024001:HMGN Proteins; C429289:HMGN5 protein, human; D039423:Integrin alpha1; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D015534:Trans-Activators