title: Natural killer cell line YT exerts cytotoxicity against CD86+ myeloma cells.
abstract: BACKGROUND
  Human NK cell lines providing an unlimited source of effector cells might be suitable for use in adoptive immunotherapy. This study determined the cytolytic activity of the human NK-like cell line YT against myeloma cell lines and primary myeloma cells.
  
  
  METHODS
  Lysis of the myeloma cell lines MM1S and U266 and of primary human myeloma cells by YT was measured using a flow-cytometric cytotoxicity assay. Furthermore, it was investigated whether the cytotoxicity correlates with the expression of CD86 on myeloma cells and the effect of different doses of IL-2 on cytolysis was tested.
  
  
  RESULTS
  YT showed killing of myeloma cell lines and primary myeloma cells. The extent of cytolysis correlated with the expression of CD86 on myeloma cells and was not augmented by preincubation of YT with high dose of IL-2.
  
  
  CONCLUSIONS
  The human NK-like cell line YT could be useful in immunotherapy of patients with CD86(+) multiple myeloma.
authors.affiliation: Medical Clinic of Oncology and Hematology, Charité-Universitätsmedizin Berlin, Campus Mitte, 10117 Berlin, Germany.
authors.forename: Ulf
authors.initials: U
authors.lastname: Harnack
authors.forename: Heiko
authors.initials: H
authors.lastname: Johnen
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pecher
mesh_terms: D018122:B7-1 Antigen / Q000096:biosynthesis / Q000276:immunology; D051940:B7-2 Antigen / Q000096:biosynthesis / Q000276:immunology*; D002460:Cell Line; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology / Q000628:therapy*
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D007376:Interleukin-2