title: A novel, sensitive, reusable and low potential acetylcholinesterase biosensor for chlorpyrifos based on 1-butyl-3-methylimidazolium tetrafluoroborate/multiwalled carbon nanotubes gel.
abstract: A novel, low potential and highly sensitive acetylcholinesterase (AChE) biosensor was developed based on 1-butyl-3-methylimidazolium tetrafluoroborate/multiwalled carbon nanotube composite gel thiocholine sensor. Composite gel promoted electron transfer reaction at a lower potential (+50 mV) and catalyzed electrochemical oxidation of thiocholine with high sensitivity. AChE was immobilized in sol-gel matrix that provides a good support for enzyme without any inhibition effect from the ionic liquid. The amount of immobilized enzyme and incubation time with chlorpyrifos were optimized. Chlorpyrifos could be determined in the range of 10(-8)-10(-6)M with a detection limit of 4 nM. Fast and efficient enzyme reactivation was obtained at low obidoxime concentration (0.1mM). Moreover, the biosensor exhibited a good stability and reproducibility and could be use for multiple determinations of pesticide with no loss of the enzyme activity.
authors.affiliation: LaborQ, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania.
authors.forename: Lucian-Gabriel
authors.initials: LG
authors.lastname: Zamfir
authors.forename: Lucian
authors.initials: L
authors.lastname: Rotariu
authors.forename: Camelia
authors.initials: C
authors.lastname: Bala
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004390:Chlorpyrifos / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005782:Gels; D007093:Imidazoles / Q000737:chemistry*; D007306:Insecticides / Q000032:analysis*; D052578:Ionic Liquids / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D004800:Enzymes, Immobilized; D005782:Gels; D007093:Imidazoles; D007306:Insecticides; D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D000110:Acetylcholinesterase; D004390:Chlorpyrifos