title: Effects of combined treatment of sodium hypochlorite/ionizing radiation and addition of vitamin B(1) on microbial flora of oyster and short-necked clam.
abstract: To determine the synergistic disinfection effect of the combined treatments of sodium hypochlorite (NaClO), irradiation, and vitamin B(1), the bactericidal effects of the treatments on natural microflora of oyster and short-necked clam were investigated. Then, bacteria isolated from the samples were identified by 16S rDNA sequencing. Oyster and short-necked clam were mainly contaminated with Vibrio spp. and Bacillus spp. Total number of aerobic bacteria ranged from 10(2) to 10(4) colony forming units (CFU)/g initially. More than 100 mg/L of NaClO with 1000 mg/L vitamin B(1) and 2 kGy irradiation treatment for oyster and short-necked clam can reduce the total aerobic bacteria to the level of lower than a detection limit (10 CFU/g). Synergistic effects were observed for all combined treatment against natural microflora. The results suggest that a significant synergistic benefit can be achieved by a combination of NaClO-ionizing radiation treatment with the addition of vitamin B(1) to reduce the microbial population contaminated in oyster and short-necked clam.
authors.affiliation: Department of Food Science and Technology, Chung-Ang University, Anseong, Republic of Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Ji-Hyoung
authors.initials: JH
authors.lastname: Ha
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
authors.forename: Jiyong
authors.initials: J
authors.lastname: Park
authors.forename: Sang-Do
authors.initials: SD
authors.lastname: Ha
mesh_terms: D060054:Agrobacterium / Q000187:drug effects / Q000302:isolation & purification / Q000528:radiation effects; D000818:Animals; D001407:Bacillus / Q000187:drug effects / Q000302:isolation & purification / Q000528:radiation effects; D049872:Bivalvia / Q000382:microbiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods; D004357:Drug Synergism; D005506:Food Contamination; D005514:Food Irradiation / Q000379:methods; D005516:Food Microbiology; D005720:Gamma Rays; D007723:Korea; D010125:Ostreidae / Q000382:microbiology*; D017747:Seafood / Q000382:microbiology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects / Q000302:isolation & purification / Q000528:radiation effects; D013831:Thiamine / Q000494:pharmacology*; D014733:Vibrio / Q000187:drug effects / Q000302:isolation & purification / Q000528:radiation effects; D014803:Vitamin B Complex / Q000494:pharmacology
chemical_list: D004202:Disinfectants; D014803:Vitamin B Complex; D012973:Sodium Hypochlorite; D013831:Thiamine