title: Strand 6B deformation and residues exposure towards N-terminal end of helix B during proteinase inhibition by Serpins.
abstract: Serine Protease inhibitors (Serpins) like antithrombin, antitrypsin, neuroserpin, antichymotrypsin, protein C-inhibitor and plasminogen activator inhibitor is involved in important biological functions like blood coagulation, fibrinolysis, inflammation, cell migration and complement activation. Serpins native state is metastable, which undergoes transformation to a more stable state during the process of protease inhibition. Serpins are prone to conformation defects, however little is known about the factors and mechanisms which promote its conformational change and misfolding. Helix B region in serpins is with several point mutations which result in pathological conditions due to polymerization. Helix B analysis for residue burial and cavity was undertaken to understand its role in serpin structure function. A structural overlap and an accessible surface area analysis showed the deformation of strand 6B and exposure of helix B at N-terminal end in cleaved conformation but not in the native and latent conformation of various inhibitory serpins. A cleaved polymer like conformation of antitrypsin also showed deformation of s6B and helix B exposure. Cavity analysis showed that helix B residues were part of the largest cavity in most of the serpins in the native state which increase in size during the transformation to cleaved and latent states. These data for the first time show the importance of strand 6B deformation and exposure of helix B in smooth insertion of the reactive center loop during serpin inhibition and indicate that helix B exposure due to variants may increase its polymer propensity.
  
  
  BACKGROUND
  serpin -serine protease inhibitors RCL -reactive center loop ASA -accessible surface area.
authors.affiliation: Department of Biosciences, Jamia Millia Islamia University, Jamia Nagar, New Delhi-110025, India; Mohamad Aman Jairajpuri- ;
authors.forename: Poonam
authors.initials: P
authors.lastname: Singh
authors.forename: Mohamad Aman
authors.initials: MA
authors.lastname: Jairajpuri