title: Mitochondrial DNA alterations in colorectal cancer cell lines.
abstract: Somatic mutations of mitochondrial DNA (mtDNA) have been reported in different types of cancers and are suggested to play roles in metastasis, cancer development and response to anticancer agents. To predict potential roles of mtDNA alterations in colorectal cancer, we determined the entire mtDNA sequence of eleven human-derived colorectal cancer cell lines and compared with the revised Cambridge Reference Sequence to identify nucleotide alterations. Four homoplasmic and six heteroplasmic alterations were found to be novel. Among them, homoplasmic G6709A (MT-CO1) and G14804A (MT-CYB) alterations cause amino acid changes in the highly conserved residues. Heteroplasmic G1576A (MT-RNR1) and G2975A (MT-RNR2) alterations are expected to make the stem structure of mitochondrial ribosomal RNAs unstable. These nucleotide alterations are candidates that could play important roles in cancer.
authors.affiliation: Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School, Kanagawa, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Chihara
authors.forename: Taku
authors.initials: T
authors.lastname: Amo
authors.forename: Akira
authors.initials: A
authors.lastname: Tokunaga
authors.forename: Ryo
authors.initials: R
authors.lastname: Yuzuriha
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Wolf
authors.forename: Sadamitsu
authors.initials: S
authors.lastname: Asoh
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Eiji
authors.initials: E
authors.lastname: Uchida
authors.forename: Shigeo
authors.initials: S
authors.lastname: Ohta
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D045303:Cytochromes b; D003576:Electron Transport Complex IV