title: An elastase-dependent attenuated heterologous swine influenza virus protects against pandemic H1N1 2009 influenza challenge in swine.
abstract: Influenza virus infections continue to cause production losses in the agricultural industry in addition to being a human public health concern. The primary method to control influenza is through vaccination. However, currently used killed influenza virus vaccines must be closely matched to the challenge virus. The ability of an elastase-dependent live attenuated influenza A virus was evaluated to protect pigs against the pandemic H1N1 2009 influenza virus. Pigs vaccinated intranasally or intratracheally with the elastase-dependent swine influenza virus (SIV) vaccine had significantly reduced macroscopic and microscopic lung lesions and lower viral loads in the lung and in nasal swabs. Thus, elastase-dependent SIV mutants can be used as live-virus vaccines against swine influenza in pigs. In addition, low levels of cross-neutralizing antibodies to H1N1 2009 were elicited prior to challenge by the swine adapted H1N1 avian strain vaccine.
authors.affiliation: National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada R3E 3M4. shawn.babiuk@inspection.gc.ca
authors.forename: Shawn
authors.initials: S
authors.lastname: Babiuk
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Masic
authors.forename: Jill
authors.initials: J
authors.lastname: Graham
authors.forename: James
authors.initials: J
authors.lastname: Neufeld
authors.forename: Melanie
authors.initials: M
authors.lastname: van der Loop
authors.forename: John
authors.initials: J
authors.lastname: Copps
authors.forename: Yohannes
authors.initials: Y
authors.lastname: Berhane
authors.forename: John
authors.initials: J
authors.lastname: Pasick
authors.forename: Andy
authors.initials: A
authors.lastname: Potter
authors.forename: Lorne A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: Hana
authors.initials: H
authors.lastname: Weingartl
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000280:Administration, Inhalation; D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D056738:Cross Protection; D003429:Cross Reactions; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008168:Lung / Q000473:pathology / Q000821:virology; D009296:Nasal Cavity / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D019562:Viral Load
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007252:Influenza Vaccines