title: Muscle-derived precursor cells isolated on the basis of differential adhesion properties respond differently to capillary flow.
abstract: Capillary shear stress can improve osteogenic differentiation in muscle-derived precursor cells (MDPCs). This has implications for large-scale bioprocessing of cell therapies where capillary transfer is needed. The recovery, viability, and osteogenic differentiation potential of two subsets of MDPCs, early-adherent pre-plate 1 (PP1) and late-adherent PP3 populations, have been examined: PP1 MDPCs produced a greater degree of osteogenic differentiation than PP3 MDPCs, quantified by Alizarin Red S staining intensity (P < 0.05). For both cell populations, capillary flow-induced significant increases in Alizarin Red S staining (P < 0.05). However, PP1 cells were more susceptible to capillary flow-induced damage than PP3 cells and this was dependent on duration of exposure. Overall, results indicate that different cell subsets, even from within a single tissue, can respond variably to capillary shear stress, necessitating its precise monitoring and control.
authors.affiliation: Regenerative Medicine Bioprocessing Unit, Department of Biochemical Engineering, University College London, Torrington Place, London, UK. minal.patel@ucl.ac.uk
authors.forename: Minal
authors.initials: M
authors.lastname: Patel
authors.forename: Hayley
authors.initials: H
authors.lastname: Mulhall
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-Quatani
authors.forename: Mark
authors.initials: M
authors.lastname: Lewis
authors.forename: Ivan
authors.initials: I
authors.lastname: Wall
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D002196:Capillaries / Q000502:physiology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D009132:Muscles / Q000166:cytology*; D032446:Myoblasts / Q000502:physiology*; D051381:Rats