title: [Screening of the candidate proteins interacting with TaFRA].
abstract: F-box protein is an important subunit of SCF complex, an E3 ligase in ubiquitin system, and its function is determined through mediating the specific recognition and combining with substrate protein. TaFRA (F-box protein related to abiotic stress) was identified by RACE based on the fragments diferently expressing in wheat seedling exposed to salt stress and encodes an F-box protein. In this study, pBD-TaFRA bait expression vector was constructed, and cDNA+pGAD+pBD was directly co-transformed into yeast hybrid system to screen condidate proteins interacting with TaFRA. Fourty-four candidate proteins were obtained, in which 32 were known proteins and transcript factors related to stress tolerance such as thioredoxin, metallothinein, ATP synthase, and serine/threonine protein kinase etc. This indicates that TaFRA participates in stress response through regulating above condidate genes, which will provide basis for revealing the mechanism of TaFRA reaction to abiotic stress.
authors.affiliation: Chinese Academy of Agricultural Sciences, Beijing 100081, China. wen_xiaojie@163.com
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Wen
authors.forename: Chen-Yang
authors.initials: CY
authors.lastname: Hao
authors.forename: Wen
authors.initials: W
authors.lastname: Pu
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Xue-Yong
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D003001:Cloning, Molecular; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques
chemical_list: D044783:F-Box Proteins