title: Identification of LI-F type antibiotics and di-n-butyl phthalate produced by Paenibacillus polymyxa.
abstract: Paenibacillus polymyxa strain JSa-9, a soil isolate that displayed antibacterial and antifungal activities in vitro, had been found to produce two types of antimicrobial substances. The two compounds were extracted from the fermentation broth of JSa-9 using ethyl acetate and subsequently purified by high performance liquid chromatography. By means of liquid chromatography-mass spectrometry and tandem mass spectrometry analysis, one of two antagonistic compounds was determined as di-n-butyl phthalate. And another was characterized as a mixture of related peptides of molecular masses of 883, 897, 911, 947, and 961Da, with the most likely structure of them determined to be a cyclic depsipeptide with an unusual 15-guanidino-3-hydroxypentadecanoic acid moiety bound to a free amino group. These peptides were therefore members of the LI-F group of cyclic depsipeptides.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture of China, 1 Weigang Nanjing 210095, P.R China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Deng
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Lu
authors.forename: Fengxia
authors.initials: F
authors.lastname: Lu
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Haizhen
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bie
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D003993:Dibutyl Phthalate / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D056507:Paenibacillus / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media; D003993:Dibutyl Phthalate