title: Nuclear factor-κB is involved in the phenotype loss of parvalbumin-interneurons in vitro.
abstract: The phenotype loss of parvalbumin-containing interneurons, characterized by decreased parvalbumin expression, has been observed in schizophrenic patients. Overproduction of intraneuronal reactive oxygen species leads to such a phenotype loss. Nuclear factor-κB (NF-κB) activation is both a target and a regulator of intracellular oxidative stress response, suggesting its involvement in the parvalbumin regulation. This study was carried out to investigate the role of the NF-κB activation in the ketamine-induced phenotype loss of parvalbumin-interneurons in vitro. Ketamine was applied to primary neuronal cultures to successfully evoke the production of increased reactive oxygen species and decreased parvalbumin expression in parvalbumin-interneurons, which was invalid in the presence of a NF-κB inhibitor, SN50 or Bay11-7082. These results suggest potential links among NF-κB activation, oxidative stress, and parvalbumin-interneurons in vitro.
authors.affiliation: Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists / Q000633:toxicity; D007395:Interneurons / Q000187:drug effects / Q000378:metabolism*; D007649:Ketamine / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010320:Parvalbumins / Q000172:deficiency* / Q000235:genetics; D010641:Phenotype
chemical_list: D018691:Excitatory Amino Acid Antagonists; D016328:NF-kappa B; D010320:Parvalbumins; D007649:Ketamine