title: Mutation Screening Using PCR-SSCP : Silver Staining and Isotopic Protocols.
abstract: Screening for mutations prior to sequencing can reduce the time and costs of identifying mutations. When the DNA sequence is known, the technique of detecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (1). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insulin receptor (2), GLUT 4 (3), glucokinase (4), and the mitochondrial genome (5).
authors.affiliation: Unit of Metabolic Medicine, St. Mary's Hospital Medical School Paddington, London, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Saker