title: Molecular epidemiology of KPC-2- producing Klebsiella pneumoniae isolates in Brazil: the predominance of sequence type 437.
abstract: The aim of this study was to investigate the genetic relatedness of 57 KPC-2-producing Klebsiella pneumoniae isolates from 5 states in Brazil, during 2006-2009. Pulse-field gel electrophoresis analysis identified 10 pulsotypes. The pulsotype designated as Kp-RJ (Klebsiella pneumoniae-Rio de Janeiro) was the dominant clone found in the states of Rio de Janeiro and Espírito Santo. Multilocus sequence typing of Kp-RJ assigned it to ST 437. Sequence types ST11, ST16, ST25, ST70, ST101, ST105, ST423, ST442, and ST443 were also identified. These results indicate the dissemination of a successful KPC-producing clone (ST437) in Brazil, which is a single locus variant of ST258.
authors.affiliation: Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil 4365, 21045-900, Rio de Janeiro, Brazil.
authors.forename: Liliane M
authors.initials: LM
authors.lastname: Seki
authors.forename: Polyana S
authors.initials: PS
authors.lastname: Pereira
authors.forename: Maria da Penha A H
authors.initials: Mda P
authors.lastname: de Souza
authors.forename: Magda de S
authors.initials: Mde S
authors.lastname: Conceição
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Marques
authors.forename: Carlos Oliveira
authors.initials: CO
authors.lastname: Porto
authors.forename: Elvira Maria L
authors.initials: EM
authors.lastname: Colnago
authors.forename: Carlene de F M
authors.initials: Cde F
authors.lastname: Alves
authors.forename: Deize
authors.initials: D
authors.lastname: Gomes
authors.forename: Ana Paula D A Carvalho
authors.initials: AP
authors.lastname: Assef
authors.forename: Ørjan
authors.initials: Ø
authors.lastname: Samuelsen
authors.forename: Marise D
authors.initials: MD
authors.lastname: Asensi
mesh_terms: D001938:Brazil / Q000453:epidemiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017720:Molecular Epidemiology; D058889:Molecular Typing; D058885:Multilocus Sequence Typing; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae