title: MARCH-I expression in cord blood CD34+KDR+ cells.
abstract: Hematopoietic stem cells transplantation has been successfully used in the treatment of patients with hematological malignances. A better knowledge of the mechanisms beyond their ability to completely repopulate the entire hematopoietic system would help in the treatment of hematological diseases. For this reason we focused our studies on a cell population that has been demonstrated to have some peculiar characteristics among the stem cells: CD34+KDR+ cells. These cells, an extremely rare population among the CD34 (0.1%-0.5%) cells, have been demonstrated from different groups to have the potential to give rise to the hematopoietic and endothelial lineage. By a subtraction library approach we found different sequences more expressed in CD34+KDR+ than their CD34+KDR- counterpart. In particular, we found an open reading frame correspondent to a newly characterized E3 ligase, MARCH-I. This gene is part of a recently described family involved in immune response modulation through the proteosomal mediated degradation. MARCH-I expression in stem cells could be important for their intrinsic immune properties.
authors.affiliation: Department Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, Catania, Italy. iannolo@yahoo.com
authors.forename: Gioacchin
authors.initials: G
authors.lastname: Iannolo
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Sciuto
authors.forename: Cristina
authors.initials: C
authors.lastname: La Rosa
authors.forename: Concetta
authors.initials: C
authors.lastname: Conticello
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044767:Ubiquitin-Protein Ligases; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D002352:Carrier Proteins; D008565:Membrane Proteins; C502926:MARCHF2 protein, human; D044767:Ubiquitin-Protein Ligases; D040301:Vascular Endothelial Growth Factor Receptor-2