title: Refolding, crystallization and preliminary X-ray crystallographic study of the whole extracellular regions of nectins.
abstract: The nectin family of Ca2+-independent immunoglobulin-like cell-cell adhesion molecules contains four members. Nectins, which have three Ig-like domains in their extracellular region, form cell-cell adherens junctions cooperatively with cadherins. The whole extracellular regions of nectin-1 (nectin-1-EC) and nectin-2 (nectin-2-EC) were expressed in Escherichia coli as inclusion bodies, solubilized in 8 M urea and then refolded by rapid dilution into refolding solution. The refolded proteins were subsequently purified by three chromatographic steps and crystallized using the hanging-drop vapour-diffusion method. The nectin-1-EC crystals belonged to space group P2(1)3 and the nectin-2-EC crystals belonged to space group P6(1)22 or P6(5)22.
authors.affiliation: Laboratory of Supramolecular Crystallography, Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita 565-0871, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Narita
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Sakisaka
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
authors.forename: Mamoru
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D000075983:Nectins; D017510:Protein Folding; D017434:Protein Structure, Tertiary
chemical_list: D015815:Cell Adhesion Molecules; C000619010:NECTIN1 protein, human; C000619015:Nectin1 protein, mouse; D000075983:Nectins