title: Expanding the versatility of phage display I: efficient display of peptide-tags on protein VII of the filamentous phage.
abstract: BACKGROUND
  Phage display is a platform for selection of specific binding molecules and this is a clear-cut motivation for increasing its performance. Polypeptides are normally displayed as fusions to the major coat protein VIII (pVIII), or the minor coat protein III (pIII). Display on other coat proteins such as pVII allows for display of heterologous peptide sequences on the virions in addition to those displayed on pIII and pVIII. In addition, pVII display is an alternative to pIII or pVIII display.
  
  
  RESULTS
  Here we demonstrate how standard pIII or pVIII display phagemids are complemented with a helper phage which supports production of virions that are tagged with octa FLAG, HIS(6) or AviTag on pVII. The periplasmic signal sequence required for pIII and pVIII display, and which has been added to pVII in earlier studies, is omitted altogether.
  
  
  CONCLUSIONS
  Tagging on pVII is an important and very useful add-on feature to standard pIII and pVII display. Any phagemid bearing a protein of interest on either pIII or pVIII can be tagged with any of the tags depending simply on choice of helper phage. We show in this paper how such tags may be utilized for immobilization and separation as well as purification and detection of monoclonal and polyclonal phage populations.
authors.affiliation: Centre for Immune Regulation, University of Oslo, Oslo, Norway. g.a.loset@imbv.uio.no
authors.forename: Geir Åge
authors.initials: GÅ
authors.lastname: Løset
authors.forename: Bjarne
authors.initials: B
authors.lastname: Bogen
authors.forename: Inger
authors.initials: I
authors.lastname: Sandlie
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D036022:Capsid Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D004526:Efficiency; D017912:Inovirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D019151:Peptide Library; D010455:Peptides; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins