title: [Analysis of thickening polysaccharides by the improved diethyldithioacetal derivatization method].
abstract: The identification test for thickening polysaccharides containing neutral saccharides and uronic acids was investigated by GC analysis of constituent monosaccharides. The reported method, in which monosaccharides were converted to diethyldithioacetal derivatives with ethanethiol followed by trimethylsilylation, was improved in terms of operability and reproducibility of GC/MS analysis. The suitability of the improved diethyldithioacetal derivatization method was determined for seven thickening polysaccharides, i.e., carob bean gum, guar gum, karaya gum, gum arabic, gum ghatti, tragacanth gum and peach gum. The samples were acid-hydrolyzed to form monosaccharides. The hydrolysates were derivatized and analyzed with GC/FID. Each sugar derivative was detected as a single peak and was well separated from others on the chromatograms. The amounts of constituent monosaccharides in thickening polysaccharides were successfully estimated. Seven polysaccharides were distinguished from each other on the basis of constituent monosaccharides. Further examination of the time period of hydrolysis of polysaccharides using peach gum showed that the optimal times were not the same for all monosaccharides. A longer time was needed to hydrolyze glucuronic acid than neutral saccharides. The findings suggest that hydrolysis time may sometimes affect the analytical results on composition of constituent monosaccharides in polysaccharides.
authors.affiliation: National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan. akiyamat@nihs.go.jp
authors.forename: Takumi
authors.initials: T
authors.lastname: Akiyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tanamoto
mesh_terms: D005503:Food Additives / Q000737:chemistry*; D005685:Galactans / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006170:Gum Arabic / Q000032:analysis; D007618:Karaya Gum / Q000032:analysis; D008351:Mannans / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis; D053149:Plant Gums / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D014144:Tragacanth / Q000032:analysis
chemical_list: D005503:Food Additives; D005685:Galactans; D008351:Mannans; D009005:Monosaccharides; D053149:Plant Gums; D011134:Polysaccharides; D006170:Gum Arabic; D007618:Karaya Gum; D014144:Tragacanth; C007894:guar gum; C017471:locust bean gum; C015253:gum ghatti