title: Anti-infectious activity of intravitreal injectable voriconazole microspheres on experimental rabbit fungal endophthalmitis caused by Aspergillus fumigatus.
abstract: The therapeutic effect of sustained intravitreal injectable voriconazole microspheres (VCZ-MS) on experimental endophthalmitis caused by Aspergillus fumigatus was investigated. VCZ-MS were prepared and evaluated. Vitrectomy was performed on rabbits after intravitreal inoculation of susceptible A. fumigatus. The animals were randomly divided into five groups, including control (no treatment after vitrectomy), vitrectomy plus voriconazole intravitreal injection, and vitrectomy plus intravitreal injection of VCZ-MS containing 0.5, 1.0, or 1.5 mg of voriconazole. The therapeutic effect was assessed at different time intervals. Voriconazole concentrations were monitored in the vitreous injected with VCZ-MS containing 1.0 mg of voriconazole. The results showed that endophthalmitis occurred in all eyes of the control group and rapidly developed into panophthalmitis. The inflammation in the voriconazole and VCZ-MS groups was mild, and in the groups treated with VCZ-MS containing 1.0 or 1.5 mg voriconazole, the inflammation was controlled, the vitreous was clear in all eyes, and there was no recurrence of endophthalmitis. Histopathological examination showed normal structures in the cured eyes, while most uncured eyes were atrophied. Therefore, it can be concluded that an intravitreal injection of VCZ-MS in addition to vitrectomy is an effective treatment for A. fumigatus-induced endophthalmitis in rabbits.
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Qingdao 266071, China. wuxianggen@126.com
authors.forename: Wu
authors.initials: W
authors.lastname: Xiang-Gen
authors.forename: Yang
authors.initials: Y
authors.lastname: Li-Na
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.forename: Jiang
authors.initials: J
authors.lastname: Hao-Ran
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D001228:Aspergillosis / Q000188:drug therapy* / Q000473:pathology; D001232:Aspergillus fumigatus / Q000187:drug effects*; D003692:Delayed-Action Preparations / Q000737:chemistry; D009877:Endophthalmitis / Q000188:drug therapy* / Q000473:pathology; D005123:Eye / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D006801:Humans; D058449:Intravitreal Injections; D008863:Microspheres; D011743:Pyrimidines / Q000008:administration & dosage* / Q000627:therapeutic use; D011817:Rabbits; D014230:Triazoles / Q000008:administration & dosage* / Q000627:therapeutic use; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D003692:Delayed-Action Preparations; D011743:Pyrimidines; D014230:Triazoles; D065819:Voriconazole