title: [Simultaneous determination of delta-9-tetrahydrocannabinol cannabidiol and cannabinol in edible oil using ultra performance liquid chromatography-tandem mass spectrometry].
abstract: A method for the simultaneous determination of delta-9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in edible oil was developed using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The target compounds were extracted with methanol, purified by an LC-Alumina-N solid phase extraction cartridge, separated and detected by the UPLC-MS/MS. Quantitative analysis was corrected by an isotope internal standard method using delta-9-THC-D3 as internal standard. Average recoveries for the target compounds varied from 68.0% to 101.6% with the relative standard deviations ranging from 7.0% to 20.1% at three spiked levels. The limits of detection (LOD) of the method were from 0.06-0.17 microg/kg and the limits of quantification (LOQ) were in the range of 0.20-0.52 microg/kg. The results showed that the method is able to meet the requirements for the simultaneous determination of THC, CBD and CBN in edible oil.
authors.affiliation: Ningbo Academy of Product Quality Supervision & Inspection, Ningbo 315041, China.
authors.forename: Aizhi
authors.initials: A
authors.lastname: Zhang
authors.forename: Quanlin
authors.initials: Q
authors.lastname: Wang
authors.forename: Shijie
authors.initials: S
authors.lastname: Mo
mesh_terms: D002185:Cannabidiol / Q000032:analysis*; D002187:Cannabinol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D010938:Plant Oils; D002185:Cannabidiol; D013759:Dronabinol; D002187:Cannabinol; C025351:delta(9)-tetrahydrocannabinolic acid