title: New biotech applications from evolved D-amino acid oxidases.
abstract: D-Amino acid oxidase (DAAO) is a well-known flavoenzyme that catalyzes the oxygen-dependent oxidative deamination of amino acid D-isomers with absolute stereospecificity, which results in α-keto acids, ammonia and hydrogen peroxide. Recently, the extraordinary functional plasticity of DAAO has become evident; in turn, boosting research on this flavoprotein. Protein engineering has allowed for a redesign of DAAO substrate specificity, oxygen affinity, cofactor binding, stability, and oligomeric state. We review recent developments in utilizing DAAO, including as a biocatalyst for resolving racemic amino acid mixtures, as a tool for biosensing, and as a new mechanism of herbicide resistance. Perspectives for future biotechnological applications of this oxidative biocatalyst are also outlined.
authors.affiliation: Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, Varese, Italy. loredano.pollegioni@uninsubria.it
authors.forename: Loredano
authors.initials: L
authors.lastname: Pollegioni
authors.forename: Gianluca
authors.initials: G
authors.lastname: Molla
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D003067:Coenzymes / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007651:Keto Acids / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D015202:Protein Engineering; D055550:Protein Stability; D013379:Substrate Specificity
chemical_list: D000596:Amino Acids; D003067:Coenzymes; D007651:Keto Acids; D000641:Ammonia; D006861:Hydrogen Peroxide; D003605:D-Amino-Acid Oxidase; D010100:Oxygen