title: Detection of bacterial DNA in serum and ascitic fluid of asymptomatic outpatients with cirrhosis and non-neutrocytic ascites.
abstract: OBJECTIVE
  Bacterial DNA (bactDNA) has been found in serum and ascitic fluid (AF) of 30-40% of hospitalized patients with cirrhosis and non-neutrocytic ascites, but its prevalence in outpatients is unknown. The aim of this prospective study was to investigate the presence of bactDNA in AF and serum among cirrhotic outpatients with non-neutrocytic ascites.
  
  
  METHODS
  Thirty-one consecutive patients with cirrhosis and non-neutrocytic ascites, who underwent therapeutic paracentesis in our outpatient clinic, were enrolled over a 13-week period. Of these patients, 13 had a single paracentesis and 18 patients had several consecutive paracenteses (2-10) over the study period. Overall, 98 serum and non-neutrocytic AF specimens were obtained and tested for the presence of bactDNA by polymerase chain reaction amplification of the 16S ribosomal RNA gene.
  
  
  RESULTS
  The main causes of cirrhosis were alcohol (53.5%) and hepatitis C (30%). The median MELD score was 16 and there were 54.8% Child-Pugh C patients. BactDNA was negative in all samples from 28 of the 31 patients, including 15 patients with several paracentesis. One patient had a single AF sample culture positive and bactDNA positive for Streptococcus mitis, whereas the simultaneous blood sample was negative. For each of the last two patients, DNA from Lactococcus lactis was detected in a single blood sample but not in the simultaneous AF sample.
  
  
  CONCLUSIONS
  In contrast to that reported previously in hospitalized patients, bactDNA is rarely detected in serum and AF of outpatients with cirrhosis and non-neutrocytic ascites.
authors.affiliation: Service d'Hépatologie, Hôpital AP-HP Beaujon, Clichy, France. thomas.serste@skynet.be
authors.forename: Thomas
authors.initials: T
authors.lastname: Sersté
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bert
authors.forename: Véronique
authors.initials: V
authors.lastname: Leflon-Guibout
authors.forename: Chantal
authors.initials: C
authors.lastname: Chauvet
authors.forename: Estelle
authors.initials: E
authors.lastname: Marcon
authors.forename: Tarik
authors.initials: T
authors.lastname: Asselah
authors.forename: Claire
authors.initials: C
authors.lastname: Francoz
authors.forename: François
authors.initials: F
authors.lastname: Durand
authors.forename: Didier
authors.initials: D
authors.lastname: Lebrec
authors.forename: Dominique
authors.initials: D
authors.lastname: Valla
authors.forename: Richard
authors.initials: R
authors.lastname: Moreau
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Nicolas-Chanoine
mesh_terms: D001201:Ascites / Q000097:blood / Q000382:microbiology*; D001202:Ascitic Fluid / Q000737:chemistry*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D005260:Female; D005602:France; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000382:microbiology*; D008297:Male; D010045:Outpatients; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S