title: Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme.
abstract: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in catecholamine biosynthesis, and its N-terminus plays a critical role in the intracellular stability of the enzyme. In the present study, we investigated the mechanism by which the N-terminal region of TH affects this stability. TH molecules phosphorylated at their Ser31 and Ser40 were localized predominantly in the cytoplasm of PC12D cells. However, those molecules phosphorylated at Ser19 were found mainly in the nucleus, whereas they seemed to be negligible in the cytoplasm. The inhibition of proteasomes increased the quantity of TH molecules phosphorylated at their Ser19 and Ser40, although it did not increase that of TH molecules or that of TH phosphorylated at its Ser31. The inhibition of autophagy did not affect the amount of the TH molecule or that of its three phosphorylated forms. Deletion mutants of human TH type-1 lacking the N-terminal region containing the three phosphorylation sites possessed high stability of the enzyme in PC12D cells. These results suggest that the phosphorylation of the N-terminal portion of TH regulates the degradation of this enzyme by the ubiquitin-proteasome pathway.
authors.affiliation: Department of Physiology, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan. anakashi@fujita-hu.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Nakashima
authors.forename: Keiji
authors.initials: K
authors.lastname: Mori
authors.forename: Yoko S
authors.initials: YS
authors.lastname: Kaneko
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hayashi
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagatsu
authors.forename: Akira
authors.initials: A
authors.lastname: Ota
mesh_terms: D000818:Animals; D001343:Autophagy; D006801:Humans; D008247:Lysosomes / Q000201:enzymology; D016716:PC12 Cells; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000235:genetics; D051381:Rats; D017384:Sequence Deletion; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D025801:Ubiquitin; D014446:Tyrosine 3-Monooxygenase; D046988:Proteasome Endopeptidase Complex