title: Evolutionary sequence of cytogenetic aberrations during the oncogenesis of plasma cell disorders. Direct evidence at single cell level.
abstract: Bone marrow specimens from 185 patients with plasma cell disorders (PCD) were investigated by fluorescence in situ hybridization (FISH) in order to determine the temporal sequence of cytogenetic aberrations. In 25 cases combined FISH analysis has also been performed at single cell level. Clonal evolution was observed in 16% of cases. The Δ13 was preceded by t(4;14)(p16;q32) and t(14;16)(q32;q23) translocations. Deletion of p53 gene was a secondary aberration compared to Δ13 and t(11;14)(q13;q32) translocation. In 22% of all cases with recurrent IGH translocation, this aberration was presented only in a subset of purified plasma cells questioning its initiating role.
authors.affiliation: Department of Pathology, University of Pécs, Pécs, Hungary.
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Nagy
authors.forename: Béla
authors.initials: B
authors.lastname: Kajtár
authors.forename: Pál
authors.initials: P
authors.lastname: Jáksó
authors.forename: Mariann
authors.initials: M
authors.lastname: Dávid
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Kosztolányi
authors.forename: Judit
authors.initials: J
authors.lastname: Hermesz
authors.forename: László
authors.initials: L
authors.lastname: Kereskai
authors.forename: László
authors.initials: L
authors.lastname: Pajor
authors.forename: Donát
authors.initials: D
authors.lastname: Alpár
mesh_terms: D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007952:Leukemia, Plasma Cell / Q000235:genetics* / Q000473:pathology; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics
chemical_list: D007143:Immunoglobulin Heavy Chains