title: In situ hybridization of cells and tissue sections.
abstract: In situ hybridization (ISH) has become a powerful and versatile method for the detection and localization of nucleic acid sequences within cells or tissue preparations. The technique provides a high degree of spatial information; specific DNA or RNA sequences can be located within a small subpopulation of cells, chromosomes or even individual cells within a tissue sample. ISH can be used to identify sites of gene expression, identify and localize viral infection, follow changes in specific mRNA synthesis, and map chromosomes.
authors.affiliation: Division of Cell and Molecular Biology, The Institute of Child Health University of London, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Conway