title: Immunological characterization of honey proteins and identification of MRJP 1 as an IgE-binding protein.
abstract: We encountered a fourth case of honey allergy in Japan. We characterized and identified the IgE-binding proteins in honey using the serum of a honey-allergenic patient. Immunoblot analysis revealed that IgE in the patient serum specifically bound to four proteins in each honey sample. At least three of these IgE-binding proteins were N-linked glycoproteins. To identify the 60-kDa IgE-binding protein in dandelion honey, the N-terminal sequences of the fragmented protein were analyzed, revealing the protein to be major royal jelly protein 1 (MRJP 1). Three IgE-binding proteins removed of N-linked oligosaccharide showed a large reduction in IgE-binding activity as compared with the intact protein. This suggests that the carbohydrates in the IgE-binding proteins are a major epitope for patient IgE.
authors.affiliation: Department of Food and Fermentation Science, Faculty of Food and Nutrition, Beppu University, Beppu, Oita, Japan. hayashi@nm.beppu-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Nobue
authors.initials: N
authors.lastname: Takamatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.forename: Takashi
authors.initials: T
authors.lastname: Arita
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000737:chemistry / Q000502:physiology; D015153:Blotting, Western; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D037502:Galectin 3 / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D006722:Honey / Q000009:adverse effects / Q000032:analysis*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D019476:Insect Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D007564:Japan; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D000099219:Royal Jelly
chemical_list: D000485:Allergens; D000939:Epitopes; D005227:Fatty Acids; D037502:Galectin 3; D007073:Immunoglobulin E; D019476:Insect Proteins; D010446:Peptide Fragments; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D000099219:Royal Jelly