title: Preparative isolation of alkaloids from Corydalis bungeana Turcz. by high-speed counter-current chromatography using stepwise elution.
abstract: High-speed counter-current chromatography (HSCCC) was successfully applied for the preparative separation and purification of alkaloids from Corydalis bungeana Turcz. (Kudiding in Chinese) for the first time. After the measurement of partition coefficient of seven target alkaloids in the nine two-phase solvent systems composed of CHCl(3)-MeOH-(0.1 M; 0.2 M; 0.3 M) HCl (4:1.5:2; 4:2:2; 4:3:2, v/v), CHCl(3)-MeOH-0.2 M HCl (4:2:2, v/v) and CHCl(3)-MeOH-0.3 M HCl (4:3:2, v/v) were finally selected for the HSCCC separation using the first upper phase as the stationary phase and the stepwise elution of the two lower mobile phases. Consequently, sanguinarine (10 mg), corynoline (25 mg), protopine (20 mg), corynoloxine (18 mg), and 12-hydroxycorynoline (8 mg) were obtained from 200 mg of crude alkaloid extracts with purities of 94-99% as determined by HPLC. Their chemical structures were characterized on the basis of (1)H-NMR, (13)C-NMR, and LC-ESI-Q-TOF-MS/MS analyses.
authors.affiliation: Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P R China.
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Xie
authors.forename: Tanxi
authors.initials: T
authors.lastname: Cai
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Xiulan
authors.initials: X
authors.lastname: Chen
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wu
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
authors.forename: Famei
authors.initials: F
authors.lastname: Li
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D031311:Corydalis / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D012997:Solvents