title: Imaging interactions of cationic antimicrobial peptides with model lipid monolayers using X-ray spectromicroscopy.
abstract: The interaction of antimicrobial peptide anoplin with 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] lipid monolayers was imaged with atomic force microscopy, scanning transmission X-ray microscopy, and X-ray photoemission electron microscopy. X-ray absorption spectromicroscopy of the surface revealed the domains of the phase-segregated surface to be composed of 98(±5)% lipid while the matrix consisted of a ~50:50 lipid-peptide mixture. We show X-ray spectromicroscopy to be a valuable quantitative tool for label-free imaging of lipid monolayers with antimicrobial peptides at a lateral spatial resolution below 80 nm.
authors.affiliation: Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, L8S 4M1, Canada.
authors.forename: Bonnie O
authors.initials: BO
authors.lastname: Leung
authors.forename: Adam P
authors.initials: AP
authors.lastname: Hitchcock
authors.forename: Amy
authors.initials: A
authors.lastname: Won
authors.forename: Anatoli
authors.initials: A
authors.lastname: Ianoul
authors.forename: Andreas
authors.initials: A
authors.lastname: Scholl
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D018625:Microscopy, Atomic Force / Q000379:methods; D008954:Models, Biological; D010715:Phosphatidylglycerols / Q000737:chemistry / Q000378:metabolism*; D056928:X-Ray Absorption Spectroscopy / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; C030345:1,2-dipalmitoylphosphatidylglycerol