title: A comprehensive investigation of anti-inflammatory diarylheptanoids from the leaves of Alnus formosana.
abstract: This study was aimed to investigate thoroughly the diarylheptanoids in the n-BuOH soluble fraction of leaves of Alnus formosana in order to examine their anti-inflammatory activities. The application of HPLC-SPE-NMR as a preliminary chemical screening led to characterization of eleven compounds. Further separation resulted in isolation of 28 compounds, of which 10 diarylheptanoids and 2-coumaroylxyloside are new natural products. Compound 1 and alnuside A (27) were found to possess good activities against LPS-induced NO production with respective IC(50) values of 7.99 and 8.08 μM, and which were devoid of significant cytotoxicity.
authors.affiliation: School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Lai
authors.forename: Chien-Kuang
authors.initials: CK
authors.lastname: Chen
authors.forename: Wan-Wan
authors.initials: WW
authors.lastname: Lin
authors.forename: Shoei-Sheng
authors.initials: SS
authors.lastname: Lee
mesh_terms: D029661:Alnus / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D036381:Diarylheptanoids; D008070:Lipopolysaccharides; D009569:Nitric Oxide