title: Innate response of human endothelial cells infected with mycobacteria.
abstract: Endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. We analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. Intracellular growth in human umbilical vein endothelial cells (HUVECs) of three Mycobacterium species: M. tuberculosis (MTB), M. abscessus (MAB) and M. smegmatis (MSM) was analyzed. M. smegmatis was eliminated; M. abscessus had an accelerate intracellular replication and M. tuberculosis did not replicate or was eliminated. M. abscessus infection induced profound cytoskeleton rearrangements, with M. tuberculosis infection changes were less marked, and with MSM were slight. Nitric oxide (NO) production was induced differentially: M. abscessus induced the highest levels followed by M. tuberculosis and M. smegmatis; the contrary was true for reactive oxygen species (ROS) production. Only M. tuberculosis infection caused beta-1 defensin over-expression. As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells.
authors.affiliation: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Z.P. 11340, México, D.F., Mexico.
authors.forename: Blanca Estela
authors.initials: BE
authors.lastname: García-Pérez
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Villagómez-Palatto
authors.forename: Jorge I
authors.initials: JI
authors.lastname: Castañeda-Sánchez
authors.forename: Ramón M
authors.initials: RM
authors.lastname: Coral-Vázquez
authors.forename: Israel
authors.initials: I
authors.lastname: Ramírez-Sánchez
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Ordoñez-Razo
authors.forename: Julieta
authors.initials: J
authors.lastname: Luna-Herrera
mesh_terms: D058491:Bacterial Load; D003599:Cytoskeleton / Q000648:ultrastructure; D023082:Defensins / Q000032:analysis / Q000096:biosynthesis*; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology* / Q000382:microbiology* / Q000648:ultrastructure; D005260:Female; D005333:Fetus; D006790:Host-Parasite Interactions; D006801:Humans; D007113:Immunity, Innate; D008855:Microscopy, Electron, Scanning; D009161:Mycobacterium / Q000254:growth & development / Q000472:pathogenicity; D009164:Mycobacterium Infections / Q000276:immunology* / Q000382:microbiology; D020102:Mycobacterium smegmatis / Q000254:growth & development* / Q000472:pathogenicity; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000472:pathogenicity; D009569:Nitric Oxide / Q000032:analysis; D011247:Pregnancy; D061251:Primary Cell Culture; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014471:Umbilical Veins / Q000166:cytology; D014774:Virulence / Q000276:immunology
chemical_list: D023082:Defensins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide