title: Identification of genes differentially expressed by Metarhizium anisopliae growing on Locusta migratoria wings using suppression subtractive hybridization.
abstract: Insect-pathogenic fungi penetrate their hosts directly through the cuticle. To better understand this process, we identified genes that were up-regulated by Metarhizium anisopliae germinating and differentiating on Locusta migratoria wings using suppression subtractive hybridization (SSH). A total of 78 unique expressed sequence tags (ESTs) up-regulated more than twofold during fungal growth on locust wings were identified. Among these 78 ESTs, 30 (38.5%) shared significant similarity with NCBI annotated hypothetical proteins, 16 (20.5%) shared low similarity to known or predicted genes, might represent novel genes, and 32 (41.0%) shared significant similarity with known proteins that are involved in various cell and molecular processes such as cell metabolism, protein metabolism, stress response and defense, and cell structure and function. Semi-quantitative RT-PCR analysis of six randomly selected genes confirmed the SSH results, verifying the fidelity of the SSH data. The results of this study provide novel information on genes expressed during early stages of infection with M. anisopliae, and improve current understanding of fungal pathogenesis.
authors.affiliation: Genetic Engineering Research Center, College of Bioengineering, Chongqing Engineering Research Center for Fungal Insecticides and Key Lab of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing University, 174# Shazheng St., Shangpingba District, Chongqing 400030, People's Republic of China.
authors.forename: Chuanbo
authors.initials: C
authors.lastname: Zhang
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Xia
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D048188:Locusta migratoria / Q000382:microbiology*; D052981:Metarhizium / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014921:Wings, Animal / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins