title: [High performance liquid chromatographic fingerprints method for the analysis of chloroform extracts of Taxus wallichiana].
abstract: A high performance liquid chromatographic (HPLC) fingerprints method was developed for the analysis of the chloroform extracts of Taxus wallichiana. The extracts were separated on an Eurospher 100 C18 column (250 mm x 4 mm, 5 microm) with the gradient elution of methanol and water at a flow rate of 1 mL/min. The detection wavelength was set at 232 nm, and the column temperature was 30 degrees C. Under the same conditions, 10 batches of the chloroform extracts of Taxus wallichiana from different habitats were analyzed with the reference substance of 10-deacetyl baccatin III (10-DAB III). The 11 peaks were selected as the characteristic peaks, and the method of principal component analysis (PCA) was applied to analyze the fingerprints of the chloroform extracts of Taxus wallichiana. The results showed that there was an interrelated relationship between habitat and quality of Taxus wallichiana. The method has good repeatability and stability, and can be utilized as an approach for the quality control of Taxus wallichiana.
authors.affiliation: School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Li
authors.forename: Yaokang
authors.initials: Y
authors.lastname: Xiong
authors.forename: Chenhuan
authors.initials: C
authors.lastname: Yu
authors.forename: Chunchun
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control; D043823:Taxoids / Q000032:analysis*; D020946:Taxus / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D043823:Taxoids; C042213:10-deacetylbaccatine III; D002725:Chloroform