title: Placenta perfusion has hematopoietic and mesenchymal progenitor stem cell potential.
abstract: BACKGROUND
  Placenta is a valuable source of stem cells for cell therapy and future application in the field of regenerative medicine. This is due to the plasticity and the immunomodulatory effects of the stem cells that it contains. In this study we present a totally closed method for hematopoietic and nonhematopoietic stem cell isolation from human term placenta.
  
  
  METHODS
  Sixty-eight placenta units were collected and manipulated for the residual fetal blood drainage. After delivery, placenta flushing with citrate-phosphate-dextrose-adenine was evaluated.
  
  
  RESULTS
  Placenta flushing using a totally closed system led to a significant amount of hematopoietic progenitor cells and multipotent mesenchymal stem cells (MSCs) without additional microbial risk, free of maternal cell contamination.
  
  
  CONCLUSIONS
  Traditionally discarded after childbirth, the term placenta now appears to be an easily accessible and abundant source of diverse origin stem cells suitable for banking strategies and for future clinical applications, including adult therapy.
authors.affiliation: 3rd University Obstetrics and Gynaecology Clinic, Ippokration General Hospital, Medical School, Aristotle University Thessaloniki, Thessaloniki, Greece.
authors.forename: Nikos
authors.initials: N
authors.lastname: Tsagias
authors.forename: Iro
authors.initials: I
authors.lastname: Koliakos
authors.forename: Maria
authors.initials: M
authors.lastname: Lappa
authors.forename: Vasileios
authors.initials: V
authors.lastname: Karagiannis
authors.forename: George G
authors.initials: GG
authors.lastname: Koliakos
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000328:Adult; D000094902:Blood Banking / Q000379:methods; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D053686:Fetal Stem Cells / Q000166:cytology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology; D010920:Placenta / Q000098:blood supply*; D011247:Pregnancy; D020858:Tissue and Organ Harvesting / Q000379:methods*