title: Beyond natural antibodies: the power of in vitro display technologies.
abstract: In vitro display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using in vitro display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using in vitro display methods are now in the clinic, with many more waiting in the wings. Unlike immunization, in vitro display permits the use of defined selection conditions and provides immediate availability of the sequence encoding the antibody. The amenability of in vitro display to high-throughput applications broadens the prospects for their wider use in basic and applied research.
authors.affiliation: Los Alamos National Laboratory, Los Alamos, New Mexico, USA. amb@lanl.gov
authors.forename: Andrew R M
authors.initials: AR
authors.lastname: Bradbury
authors.forename: Sachdev
authors.initials: S
authors.lastname: Sidhu
authors.forename: Stefan
authors.initials: S
authors.lastname: Dübel
authors.forename: John
authors.initials: J
authors.lastname: McCafferty
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D001709:Biotechnology / Q000639:trends*; D015195:Drug Design; D019151:Peptide Library
chemical_list: D000911:Antibodies, Monoclonal; D019151:Peptide Library