title: Expression, characterization and therapeutic efficacy of chimeric Fab of anti-idiotypic antibody NP30 against Schistosoma japonicum.
abstract: The murine monoclonal anti-idiotypic antibody NP30 is a promising therapeutic antibody against Schistosoma japonicum. However, the immunogenicity of murine NP30 limits its further study and application in humans. Here the chimeric Fab of NP30 (chFab-NP30) comprising the variable regions of murine NP30 and constant regions of human antibody was assembled. chFab-NP30 was expressed and purified as a soluble and functional protein. Administration of chFab-NP30 in vivo increased the survival rate, reduced egg burdens and ameliorated organ pathology of mice with acute schistosomiasis. Our study indicated that chFab-NP30 is a promising candidate to be used as a specific and efficient recombinant antibody against acute schistosomiasis japonica. Further studies on function mechanism of chFab-NP30 needs to be carried out in the future.
authors.affiliation: Key Lab of Antibody Technique of Ministry of Health, Nanjing Medical University, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhu
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Dai
authors.forename: Yin-Chang
authors.initials: YC
authors.lastname: Zhu
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhen-Qing
authors.initials: ZQ
authors.lastname: Feng
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Guan
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage* / Q000302:isolation & purification; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D011169:Portal Vein / Q000469:parasitology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D012549:Schistosoma japonicum / Q000187:drug effects*; D012554:Schistosomiasis japonica / Q000188:drug therapy* / Q000469:parasitology
chemical_list: D000871:Anthelmintics; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins