title: Mapping of signaling networks through synthetic genetic interaction analysis by RNAi.
abstract: The analysis of synthetic genetic interaction networks can reveal how biological systems achieve a high level of complexity with a limited repertoire of components. Studies in yeast and bacteria have taken advantage of collections of deletion strains to construct matrices of quantitative interaction profiles and infer gene function. Yet comparable approaches in higher organisms have been difficult to implement in a robust manner. Here we report a method to identify genetic interactions in tissue culture cells through RNAi. By performing more than 70,000 pairwise perturbations of signaling factors, we identified >600 interactions affecting different quantitative phenotypes of Drosophila melanogaster cells. Computational analysis of this interaction matrix allowed us to reconstruct signaling pathways and identify a conserved regulator of Ras-MAPK signaling. Large-scale genetic interaction mapping by RNAi is a versatile, scalable approach for revealing gene function and the connectivity of cellular networks.
authors.affiliation: German Cancer Research Center (Deutsches Krebsforschungszentrum), Division Signaling and Functional Genomics and Heidelberg University, Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Horn
authors.forename: Thomas
authors.initials: T
authors.lastname: Sandmann
authors.forename: Bernd
authors.initials: B
authors.lastname: Fischer
authors.forename: Elin
authors.initials: E
authors.lastname: Axelsson
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Huber
authors.forename: Michael
authors.initials: M
authors.lastname: Boutros
mesh_terms: D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology; D004843:Epistasis, Genetic / Q000502:physiology*; D020869:Gene Expression Profiling; D005821:Genetic Techniques; D034622:RNA Interference; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins