title: Simultaneous determination of N-benzylpiperazine and 1-(3-trifluoromethylphenyl)piperazine in rat plasma by HPLC-fluorescence detection and its application to monitoring of these drugs.
abstract: An HPLC-fluorescence detection method for simultaneous determination of N-benzylpiperazine (BZP) and 1-(3-trifluoromethylphenyl)piperazine (TFMPP) labeled with 4-(4,5-diphenyl-1 H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was described. DIB-BZP and -TFMPP were well separated within 13 min without interference of peaks from plasma components. The lower detection limits of BZP and TFMPP at a signal-to-noise ratio of 3 were 0.9 and 4.6 ng/mL, respectively. Precisions of the proposed method for intra- and inter-day assays were less than 4.8 and 9.1% as %RSD (n =5). Furthermore, the method could be successfully applied to monitor both compounds in plasma after their sole or co-administration to rats (each dose, 2 mg/kg). Clearance of TFMPP was significantly different under the conditions (P=0.047).
authors.affiliation: Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Kozue
authors.initials: K
authors.lastname: Yamahara
authors.forename: Rie
authors.initials: R
authors.lastname: Ikeda
authors.forename: Ruri
authors.initials: R
authors.lastname: Kikura-Hanajiri
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D001565:Benzoates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D007093:Imidazoles; D057230:Limit of Detection; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: C114684:4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; D001565:Benzoates; D007093:Imidazoles; C461488:N-benzylpiperazine; D010879:Piperazines; C018406:1-(3-trifluoromethylphenyl)piperazine