title: High expression of α1,2- AND α1,6-linked fucoses on amniotic AGP as a biomarker of fetal postmaturity risk.
abstract: We aimed to analyze possible alternations in the sialylation and fucosylation of α(1)-acid glycoprotein (AGP) in pregnancies complicated by postmature syndrome. The relative amounts of sialyl- and fucosyl-glycotopes on AGP were analyzed in 49 plasma and 49 amniotic fluid samples from postmature and normal pregnancies with gestational ages of 41 to 42 weeks by lectin-ELISA using sialic acid- and fucose-specific biotinylated lectins. The amniotic AGP derived from pregnancies complicated by fetal postmaturity contained higher relative amounts of the exposed innermost α1,6- (p < 0.04) as well as outer α1,2-linked fucoses (p < 0.01) compared with normal pregnancies. These changes were limited to the fucosylation, not sialylation, of amniotic AGP, and did not appear in maternal plasma AGP. The over-fucosylation of amniotic AGP may be considered a biochemical marker to monitor pregnancies and predict postmature syndrome complications in newborns, especially in pregnancies ending after 41 weeks.
authors.affiliation: Department of Chemistry and Immunochemistry, Medical University, Wrocław, Poland. morczyk@immchem.am.wroc.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Orczyk-Pawiłowicz
authors.forename: Lidia
authors.initials: L
authors.lastname: Hirnle
authors.forename: Iwona
authors.initials: I
authors.lastname: Kątnik-Prastowska
mesh_terms: D000650:Amnion / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005643:Fucose / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D009961:Orosomucoid / Q000032:analysis*; D011247:Pregnancy; D018570:Risk Assessment
chemical_list: D015415:Biomarkers; D009961:Orosomucoid; D005643:Fucose