title: Cytoplasmic intron sequence-retaining transcripts can be dendritically targeted via ID element retrotransposons.
abstract: RNA precursors give rise to mRNA after splicing of intronic sequences traditionally thought to occur in the nucleus. Here, we show that intron sequences are retained in a number of dendritically-targeted mRNAs, by using microarray and Illumina sequencing of isolated dendritic mRNA as well as in situ hybridization. Many of the retained introns contain ID elements, a class of SINE retrotransposon. A portion of these SINEs confers dendritic targeting to exogenous and endogenous transcripts showing the necessity of ID-mediated mechanisms for the targeting of different transcripts to dendrites. ID elements are capable of selectively altering the distribution of endogenous proteins, providing a link between intronic SINEs and protein function. As such, the ID element represents a common dendritic targeting element found across multiple RNAs. Retention of intronic sequence is a more general phenomenon than previously thought and plays a functional role in the biology of the neuron, partly mediated by co-opted repetitive sequences.
authors.affiliation: Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Peter T
authors.initials: PT
authors.lastname: Buckley
authors.forename: Miler T
authors.initials: MT
authors.lastname: Lee
authors.forename: Jai-Yoon
authors.initials: JY
authors.lastname: Sul
authors.forename: Kevin Y
authors.initials: KY
authors.lastname: Miyashiro
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Bell
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Fisher
authors.forename: Junhyong
authors.initials: J
authors.lastname: Kim
authors.forename: James
authors.initials: J
authors.lastname: Eberwine
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000235:genetics* / Q000378:metabolism; D003712:Dendrites / Q000235:genetics* / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D007438:Introns / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020086:Short Interspersed Nucleotide Elements / Q000235:genetics*
chemical_list: D012333:RNA, Messenger