title: Gene expression profiling of human hepatoblastoma using archived formalin-fixed and paraffin-embedded tissues.
abstract: We elucidated the genetic profile of hepatoblastomas (HBLs) to identify diagnostic and prognostic markers. RNA was extracted from 32 formalin-fixed, paraffin-embedded HBLs and corresponding nonneoplastic liver (NNL) tissues, and cDNA-mediated annealing, selection, extension, and ligation (DASL) chip assays were performed. Immunohistochemistry was performed to confirm the expression of Yin Yang 1 (YY1) protein in HBL. Twenty-four genes that were associated with signal transduction, cell-cell adhesion, cell cycle regulation, and apoptosis were differentially expressed in HBL and NNL tissues. Two apoptosis-associated genes, MYCN and BIRC5, were highly upregulated in HBL. Eight genes, including YY1 and IGF1, were upregulated in HBL cases that had a poor prognosis. Thirty-eight genes, including YY1, were differentially expressed according to histologic differentiation of HBL, and the immunohistochemical expression of YY1 was correlated with poor HBL differentiation. Thus, using DASL chip assays, we report the gene expression profiles of HBL, which suggest new candidate prognostic and diagnostic genetic markers and putative therapeutic targets for HBL.
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, #28 Yongon-dong, Jongro-gu, Seoul, 110-799, South Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Shin
authors.forename: Kyoung-Bun
authors.initials: KB
authors.lastname: Lee
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Soo-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Han-Suk
authors.initials: HS
authors.lastname: Ryu
authors.forename: Young-Nyun
authors.initials: YN
authors.lastname: Park
authors.forename: Eunsil
authors.initials: E
authors.lastname: Yu
authors.forename: Ja-June
authors.initials: JJ
authors.lastname: Jang
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D005557:Formaldehyde; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D018197:Hepatoblastoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016612:Paraffin Embedding; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016707:Tissue Fixation
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D005557:Formaldehyde