title: Anti-anaerobic activity of serum from patients treated with tigecycline for skin/soft tissue infections.
abstract: To gain additional data concerning the anti-anaerobic activity of tigecycline in serum, we analyzed blood samples from six patients with a complicated skin/soft tissue infection who were receiving IV tigecycline 50 mg every 12 h. Venous blood samples were obtained after multiple doses of tigecycline at 1, 6 and 12 h after the initiation of a 1 h IV infusion. Sera from these samples were tested to determine serum inhibitory and bactericidal activity over time against 4 anaerobic bacteria (Bacteroides fragilis, Peptoniphilus asaccharolyticus, Prevotella bivia and Finegoldia magna). An analysis of serum titers found that tigecycline exhibited early (1 h) and prolonged (12 h) inhibitory activity against each study isolate. Moreover, it provided bactericidal activity for 12 h against these strains with the exception of F. magna. Tigecycline was found to exhibit antibacterial activity at serum concentrations below the MICs of the anaerobic bacteria tested. This finding further supports that the antimicrobial activity of tigecycline can be greater than that suggested by the free fraction of drug and that serum appears to enhance this antibacterial activity.
authors.affiliation: Michigan State University, Department of Medicine, East Lansing, 48824, USA. steing@msu.edu
authors.forename: G E
authors.initials: GE
authors.lastname: Stein
authors.forename: K L
authors.initials: KL
authors.lastname: Tyrrell
authors.forename: L A
authors.initials: LA
authors.lastname: Dybas
authors.forename: D M
authors.initials: DM
authors.lastname: Citron
authors.forename: D P
authors.initials: DP
authors.lastname: Nicolau
authors.forename: E J C
authors.initials: EJ
authors.lastname: Goldstein
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D008911:Minocycline / Q000031:analogs & derivatives* / Q000627:therapeutic use; D015610:Serum Bactericidal Test / Q000379:methods; D017192:Skin Diseases, Bacterial / Q000097:blood* / Q000188:drug therapy* / Q000382:microbiology; D018461:Soft Tissue Infections / Q000097:blood* / Q000188:drug therapy* / Q000382:microbiology; D000078304:Tigecycline
chemical_list: D000900:Anti-Bacterial Agents; D000078304:Tigecycline; D008911:Minocycline