title: Differences in daptomycin and vancomycin ex vivo behaviour can lead to false interpretation of negative blood cultures.
abstract: In clinical studies on bacteraemia, the negativity of blood cultures is an important endpoint for comparing the efficacy of different therapeutic regimens. In FAN anaerobic blood culture medium (BacT/ALERT system), daptomycin displayed increased MIC against Staphylococcus aureus and improved abolishment of its carryover effect in charcoal when compared with vancomycin. Differences between these two drugs can lead to a false interpretation of negative blood cultures. To compare different antibiotic regimens for the treatment of bacteraemia, preliminary studies are mandatory to ensure that ex vivo antibiotic behaviour is similar in the blood-culture system used.
authors.affiliation: AP-HP, Hôpital Européen Georges Pompidou, Service de Microbiologie, Paris, France. patrick.grohs@egp.aphp.fr
authors.forename: P
authors.initials: P
authors.lastname: Grohs
authors.forename: B
authors.initials: B
authors.lastname: Fantin
authors.forename: A
authors.initials: A
authors.lastname: Lefort
authors.forename: M
authors.initials: M
authors.lastname: Wolff
authors.forename: L
authors.initials: L
authors.lastname: Gutmann
authors.forename: J-L
authors.initials: JL
authors.lastname: Mainardi
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D016470:Bacteremia / Q000188:drug therapy* / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D018929:Cell Culture Techniques / Q000295:instrumentation; D002606:Charcoal; D003470:Culture Media / Q000737:chemistry; D017576:Daptomycin / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013234:Stem Cells; D014640:Vancomycin / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D002606:Charcoal; D014640:Vancomycin; D017576:Daptomycin