title: A novel solid-state electrochemiluminescence detector for capillary electrophoresis based on tris(2,2'-bipyridyl)ruthenium(II) immobilized in Nafion/PTC-NH2 composite film.
abstract: A new electrochemiluminescence (ECL) detector for capillary electrophoresis (CE) based on tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)(3)(2+)) immobilized in Nafion/PTC-NH(2) (an ammonolysis product of 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA)) composite film was presented for the first time. The Nafion/PTC-NH(2) composite film could effectively immobilize tris(2,2'-bipyridyl)ruthenium(II) via ion-exchange and electrostatic interaction. Cyclic voltammetric and ECL behavior of Nafion/PTC-NH(2)/Ru composite film was investigated compared to Nafion/Ru composite. The Nafion/PTC-NH(2)/Ru composite film exhibited good ECL stability and simple operability. Then the CE with solid-state ECL detector system was successfully used to detect sophora - a quinolizidine type - alkaloids as sophoridine (SR) and matrine (MT). The CE-ECL parameters that affected separation and detection were optimized. Under the optimized conditions, the linear range was from 2.5 × 10(-8) to 2 × 10(-6)mol/L for SR, 1.0 × 10(-8) to 1.0 × 10(-6)mol/L for MT. The detection limit (S/N=3) was estimated to be 5 × 10(-9) and 10(-9)mol/L for SR and MT, respectively. It was shown that the CE coupling with solid-state ECL detector system exhibited satisfying sensitivity of analysis.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Huijing
authors.initials: H
authors.lastname: Liu
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Chai
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuo
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan