title: Isolation and handling of recombinant vaccinia viruses.
abstract: In Chapter 20 , a general procedure was described for the construction of the intermediate vectors necessary for the insertion of foreign DNA sequences into the vaccinia virus (Vv) genome. The principles and basic methodology for the isolation of recombinant vaccinia viruses will be discussed in this chapter.
authors.affiliation: Centro Nacional de Biotechnologia, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Talavera
authors.forename: J M
authors.initials: JM
authors.lastname: Rodriguez