title: Downregulation of Pax3 expression correlates with acquired GFAP expression during NSC differentiation towards astrocytes.
abstract: Glial fibrillary acidic protein (GFAP) is a principal intermediate filament in mature astrocytes of the central nervous system (CNS), and the regulation of GFAP transcription has not been well understood yet. In the present study, we reported paired box 3 protein (Pax3) as a novel regulator of GFAP transcription, which could bind the promoter region of GFAP and down regulate the GFAP level during the serum-induced astrocyte differentiation of neural stem cells (NSCs). Moreover, the overexpression and suppression of Pax3 could inhibit and promote NSC differentiation, respectively. These data suggest that Pax3 negatively regulates GFAP expression during astrocyte differentiation in vitro.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province 226001, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Du
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Qian
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D015536:Down-Regulation; D024202:Electrophoretic Mobility Shift Assay; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000235:genetics; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
chemical_list: D005904:Glial Fibrillary Acidic Protein; D015345:Oligonucleotide Probes; D000072041:PAX3 Transcription Factor; C530820:PAX3 protein, rat; D051761:Paired Box Transcription Factors