title: Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae.
abstract: Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-time PCR assays targeting lytA and psaA genes.
authors.affiliation: Centers for Diseases Control and Prevention, Atlanta, GA 30329, USA. nroupha@emory.edu
authors.forename: Nadine
authors.initials: N
authors.lastname: Rouphael
authors.forename: Sanet
authors.initials: S
authors.lastname: Steyn
authors.forename: Mathieu
authors.initials: M
authors.lastname: Bangert
authors.forename: Jackie S
authors.initials: JS
authors.lastname: Sampson
authors.forename: Peter
authors.initials: P
authors.lastname: Adrian
authors.forename: Shabir A
authors.initials: SA
authors.lastname: Madhi
authors.forename: Keith P
authors.initials: KP
authors.lastname: Klugman
authors.forename: Edwin W
authors.initials: EW
authors.lastname: Ades
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000097:blood / Q000235:genetics; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000235:genetics*; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D011008:Pneumococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D008074:Lipoproteins; C085000:PsaA protein, Streptococcus; D009238:N-Acetylmuramoyl-L-alanine Amidase