title: Assembly of alphavirus replication complexes from RNA and protein components in a novel trans-replication system in mammalian cells.
abstract: For positive-strand RNA viruses, the viral genomic RNA also acts as an mRNA directing the translation of the replicase proteins of the virus. Replication takes place in association with cytoplasmic membranes, which are heavily modified to create specific replication compartments. Here we have expressed by plasmid DNA transfection the large replicase polyprotein of Semliki Forest virus (SFV) in mammalian cells from a nonreplicating mRNA and provided a separate RNA containing the replication signals. The replicase proteins were able to efficiently and specifically replicate the template in trans, leading to accumulation of RNA and marker gene products expressed from the template RNA. The replicase proteins and double-stranded RNA replication intermediates localized to structures similar to those seen in SFV-infected cells. Using correlative light electron microscopy (CLEM) with fluorescent marker proteins to relocate those transfected cells, in which active replication was ongoing, abundant membrane modifications, representing the replication complex spherules, were observed both at the plasma membrane and in intracellular endolysosomes. Thus, replication complexes are faithfully assembled and localized in the trans-replication system. We demonstrated, using CLEM, that the replication proteins alone or a polymerase-negative polyprotein mutant together with the template did not give rise to spherule formation. Thus, the trans-replication system is suitable for cell biological dissection and examination in a mammalian cell environment, and similar systems may be possible for other positive-strand RNA viruses.
authors.affiliation: Institute of Biotechnology, University of Helsinki, P.O. Box 56 (Viikinkaari 9), 00014 Helsinki, Finland.
authors.forename: Pirjo
authors.initials: P
authors.lastname: Spuul
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Balistreri
authors.forename: Kirsi
authors.initials: K
authors.lastname: Hellström
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Golubtsov
authors.forename: Eija
authors.initials: E
authors.lastname: Jokitalo
authors.forename: Tero
authors.initials: T
authors.lastname: Ahola
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D006224:Cricetinae; D011992:Endosomes / Q000821:virology; D008854:Microscopy, Electron / Q000379:methods; D012367:RNA, Viral / Q000378:metabolism*; D012672:Semliki forest virus / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins