title: An assessment of transcriptional changes in porcine skin exposed to bromine vapor.
abstract: Bromine is an industrial chemical that can cause severe cutaneous burns. This study was a preliminary investigation into the effect of cutaneous exposure to bromine vapor using a weanling swine burn model and microarray analysis. Ventral abdominal sites were exposed to a mean calculated bromine vapor concentration of 0.69 g L(-1) for 10 or 20 min. At 48 h postexposure, total RNA from skin samples was isolated, processed, and hybridized to Affymetrix GeneChip Porcine Genome Arrays. Expression analysis revealed that bromine vapor exposure for 10 or 20 min promoted similar transcriptional changes in the number of significantly modulated probe sets. A minimum of 83% of the probe sets was similar for both exposure times. Ingenuity pathways analysis revealed eight common biological functions among the top 10 functions of each experimental group, in which 30 genes were commonly shared among 19 significantly altered signaling pathways. Transcripts encoding heme oxygenase 1, interleukin-1β, interleukin 2 receptor gamma chain, and plasminogen activator inhibitor-1 were identified as common potential therapeutic targets for Phase II/III clinical trial or FDA-approved drugs. The present study is an initial assessment of the transcriptional responses to cutaneous bromine vapor exposure identifying molecular networks and genes that could serve as targets for developing therapeutics for bromine-induced skin injury.
authors.affiliation: Battelle Biomedical Research Center, Columbus, OH 43201, USA. rogersjv@battelle.org
authors.forename: James V
authors.initials: JV
authors.lastname: Rogers
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Price
authors.forename: Morgan Q S
authors.initials: MQ
authors.lastname: Wendling
authors.forename: Mark R
authors.initials: MR
authors.lastname: Perry
authors.forename: Frances M
authors.initials: FM
authors.lastname: Reid
authors.forename: Robyn C
authors.initials: RC
authors.lastname: Kiser
authors.forename: John S
authors.initials: JS
authors.lastname: Graham
mesh_terms: D000818:Animals; D001966:Bromine / Q000633:toxicity*; D002057:Burns, Chemical / Q000209:etiology / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D025341:Principal Component Analysis; D015398:Signal Transduction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D001966:Bromine