title: In situ hybridization.
abstract: In situ hybridization techniques involve specific annealing of labeled nucleic acid probes with complimentary cellular RNAs, and the subsequent detection of these labeled probes within fixed cells (1,2). In situ hybridization can be applied to monolayer cells, tissue sections, or whole mount tissues. It is a powerful technique for the cellular or subcellular localization of transcribed RNA, and is especially useful when antibodies recognizing a particular protein are not available for immunocytochemical staining.
authors.affiliation: Department of Ophthalmology and Neurobiology, Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA.
authors.forename: X J
authors.initials: XJ
authors.lastname: Yang