title: Rosmarinic acid inhibits proliferation and induces apoptosis of hepatic stellate cells.
abstract: Hepatic stellate cells (HSCs), activated during liver injury, are defined as the most important target in the therapy of hepatic fibrosis. In the present study, we evaluated the effect of Rosmarinic acid (RosA) on the proliferation and apoptosis in activated hepatic stellate cells (HSC-T6), which is useful to decrease this cell population. The proliferation of HSC-T6 was significantly inhibited after treated with various concentrations of RosA for different times. Flow cytometric analyses and transmission electron microscope (TEM) observations revealed that HSC-T6 treated with RosA underwent apoptosis in a time dependent manner and displayed typical apoptotic features in the cells. The phosphorylation in signal transducer and activator of transcription protein-3 (STAT3), which regulates cell survival, proliferation and differentiation in a variety of tissues, was markedly decreased as the result of Western blot assay and correlated with downregulation of CyclinD1 and B cell lymphoma/leukemia-2 (Bcl-2). In conclusion, these results suggested that RosA was able to inhibit proliferation and induce apoptosis in HSC-T6, partly due to the inhibition of phosphorylation in STAT3, which contributed to the reversal of hepatic fibrosis.
authors.affiliation: Medicine Research Center, Binzhou Medical University, Yantai 264003, China. jjinzhang@126.com
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Zhang
authors.forename: You-Lei
authors.initials: YL
authors.lastname: Wang
authors.forename: Xue-Bin
authors.initials: XB
authors.lastname: Feng
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Song
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002934:Cinnamates / Q000494:pharmacology* / Q000627:therapeutic use; D019938:Cyclin D1 / Q000378:metabolism; D053630:Depsides / Q000494:pharmacology* / Q000627:therapeutic use; D015536:Down-Regulation; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D051025:bcl-Associated Death Protein / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D019938:Cyclin D1; D053630:Depsides; D010936:Plant Extracts; D019253:Proto-Oncogene Proteins c-bcl-2; D050796:STAT3 Transcription Factor; D051025:bcl-Associated Death Protein; D000097123:Rosmarinic Acid