title: The cullin-RING ubiquitin-protein ligases.
abstract: The posttranslational addition of ubiquitin (Ub) helps control the half-life, localization, and action of many intracellular plant proteins. A primary function is the degradation of ubiquitylated proteins by the 26S proteasome, which in turn plays important housekeeping and regulatory roles by removing aberrant polypeptides and various normal short-lived regulators. Strikingly, both genetic and genomic studies reveal that Ub conjugation is extraordinarily complex in plants, with more than 1500 Ub-protein ligases (or E3s) possible that could direct the final transfer of the Ub moiety to an equally large number of targets. The cullin-RING ligases (CRLs) are a highly polymorphic E3 collection composed of a cullin backbone onto which binds carriers of activated Ub and a diverse assortment of adaptors that recruit appropriate substrates for ubiquitylation. Here, we review our current understanding of the organization and structure of CRLs in plants and their dynamics, substrates, potential functions, and evolution. The importance of CRLs is exemplified by their ability to serve as sensors of hormones and light; their essential participation in various signaling pathways; their control of the cell cycle, transcription, the stress response, self-incompatibility, and pathogen defense; and their dramatically divergent evolutionary histories in many plant lineages. Given both their organizational complexities and their critical influences, CRLs likely impact most, if not all, aspects of plant biology.
authors.affiliation: Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706-1574, USA. hua3@wisc.edu
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Hua
authors.forename: Richard D
authors.initials: RD
authors.lastname: Vierstra
mesh_terms: D002453:Cell Cycle; D044842:Cullin Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D055537:Light Signal Transduction; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism / Q000502:physiology; D017434:Protein Structure, Tertiary; D054829:RING Finger Domains; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D044842:Cullin Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease