title: [Expression of glucose transporter 1 in human laryngeal squamous cell carcinoma and its clinical significance].
abstract: OBJECTIVE
  To investigate the expression of glucose transporter 1 (GLUT1) in human laryngeal squamous cell carcinoma and its relationship to clinical pathologic factors.
  
  
  METHODS
  Tumor tissues and the peritumoral and normal laryngeal tissue were collected from 57 patients with laryngeal squamous cell carcinoma. The expression of GLUT1 was evaluated at protein and mRNA level by immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR) in relation to clinical pathologic characteristics.
  
  
  RESULTS
  GLUT1 protein and mRNA expression were significantly higher in tumoral tissues than in peritumoral and normal tissues (P < 0.01, respectively). Poorly differentiated tumor expressed higher levels of GLUT1 protein and mRNA compared to well differentiated ones (P < 0.05, respectively). Stronger GLUT1 protein and mRNA were detected in larger tumors compared with that in smaller ones (P < 0.05, respectively). There were significant differences in GLUT1 protein and mRNA expression in relation to TNM stage (P < 0.05, respectively). The expression levels of GLUT1 protein and mRNA were positively correlated with lymphatic metastasis (P < 0.05, respectively).
  
  
  CONCLUSIONS
  GLUT1 may play an important role in tumorigenesis, development, differentiation, lymphatic metastasis and prognosis of human laryngeal squamous cell carcinoma, and may be a useful marker of diagnosis and prognosis.
authors.affiliation: Department of Otorhinolaryngology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. jjg1230@163.com
authors.forename: Jianguo
authors.initials: J
authors.lastname: Jiang
authors.forename: Guohua
authors.initials: G
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Ningxin
authors.initials: N
authors.lastname: Kang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D051272:Glucose Transporter Type 1; D012333:RNA, Messenger; C495677:SLC2A1 protein, human