title: Oct4-negative multipotent adult progenitor cells and mesenchymal stem cells as regulators of T-cell alloreactivity in mice.
abstract: Multipotent adult progenitor cells (MAPC) are clinically being explored as an alternative to mesenchymal stem cells (MSC) for the immunomodulatory control of graft-versus-host disease (GvHD). Here, we performed an explorative study of the immunomodulatory potential of mouse MAPC (mMAPC), in comparison with that of MSC (mMSC) using experimental models of T-cell alloreactivity. Suppressive effects of Oct4-expressing mMAPC have been described previously; here, we studied mMAPC expressing low to no Oct4 ('mClone-3'), recently shown to be most representative for the human MAPC counterpart. mClone-3 and mMSC exhibited similar immunophenotype and in vitro immunogenic behavior. Allogeneic T-cell↔dendritic cell-proliferation assays showed strong dose-dependent T-cell-suppressive effects of both mClone-3 and mMSC. In a popliteal lymph node assay, mClone-3 and mMSC equally suppressed in vivo alloreactive T-cell expansion. We conclude that mouse MAPC and MSC exhibit similar immunosuppressive behavior in in vitro and local in vivo GvHD assays.
authors.forename: Ariane
authors.initials: A
authors.lastname: Luyckx
authors.forename: Lien
authors.initials: L
authors.lastname: De Somer
authors.forename: Sandra
authors.initials: S
authors.lastname: Jacobs
authors.forename: Omer
authors.initials: O
authors.lastname: Rutgeerts
authors.forename: Caroline
authors.initials: C
authors.lastname: Lenaerts
authors.forename: Valerie D
authors.initials: VD
authors.lastname: Roobrouck
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Verfaillie
authors.forename: Mark
authors.initials: M
authors.lastname: Waer
authors.forename: Stefaan W
authors.initials: SW
authors.lastname: Van Gool
authors.forename: An D
authors.initials: AD
authors.lastname: Billiau
mesh_terms: D053687:Adult Stem Cells / Q000276:immunology* / Q000637:transplantation; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000276:immunology / Q000628:therapy; D006801:Humans; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D007519:Isoantigens / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D039904:Pluripotent Stem Cells / Q000276:immunology* / Q000637:transplantation; D033581:Stem Cell Transplantation; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D007519:Isoantigens; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse