title: Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bert.
abstract: In the present study, we carried out a systematic research on relative antioxidant activity of aqueous leaf extract of Stevia rebaudiana. The DPPH activity of aqueous leaf extract (20, 40, 50, 100 and 200 μg/ml) was increased in a dose dependent manner, which was found in the range of 40.00-72.37% as compared to ascorbic acid 64.26-82.58%. The IC(50) values of aqueous extract and ascorbic acid in DPPH radical scavenging assay were obtained to be 83.45 and 26.75 μg/ml, respectively. Measurement of total phenolic content of the aqueous leaf extract of S. rebaudiana was achieved using Folin-Ciocalteau reagent containing 56.73 mg/g of phenolic content, which was found significantly potent when compared to reference standard gallic acid. The aqueous extract also inhibited the hydroxyl radical, nitric oxide and superoxide anions with IC(50) values of 100.86, 98.73 and 100.86 μg/ml, respectively. The greater amount of phenolic compounds leads to more potent radical scavenging effects as shown by the aqueous leaf extract of S. rebaudiana.
authors.affiliation: Department of Food Science and Technology, Yeungnam University, 214-1 Dae-dong, Gyeongsan-si, Gyeongsangbuk-do 712-749, Republic of Korea. shruti.shukla15@yahoo.com
authors.forename: Shruti
authors.initials: S
authors.lastname: Shukla
authors.forename: Archana
authors.initials: A
authors.lastname: Mehta
authors.forename: Pradeep
authors.initials: P
authors.lastname: Mehta
authors.forename: Vivek K
authors.initials: VK
authors.lastname: Bajpai
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D017665:Hydroxyl Radical / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D036625:Stevia / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl