title: Qualitative PCR method for Roundup Ready soybean: interlaboratory study.
abstract: Quantitative and qualitative methods based on PCR have been developed for genetically modified organisms (GMO). Interlaboratory studies were previously conducted for GMO quantitative methods; in this study, an interlaboratory study was conducted for a qualitative method for a GM soybean, Roundup Ready soy (RR soy), with primer pairs designed for the quantitative method of RR soy studied previously. Fourteen laboratories in Japan participated. Each participant extracted DNA from 1.0 g each of the soy samples containing 0, 0.05, and 0.10% of RR soy, and performed PCR with primer pairs for an internal control gene (Le1) and RR soy followed by agarose gel electrophoresis. The PCR product amplified in this PCR system for Le1 was detected from all samples. The sensitivity, specificity, and false-negative and false-positive rates of the method were obtained from the results of RR soy detection. False-negative rates at the level of 0.05 and 0.10% of the RR soy samples were 6.0 and 2.3%, respectively, revealing that the LOD of the method was somewhat below 0.10%. The current study demonstrated that the qualitative method would be practical for monitoring the labeling system of GM soy in kernel lots.
authors.affiliation: Food and Agricultural Materials Inspection Center, 2-1 Shintoshin, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kodama
authors.forename: Masaki
authors.initials: M
authors.lastname: Kasahara
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Minegishi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Futo
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sawada
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Watai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kurosawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Furui
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hino
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
mesh_terms: D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005998:Glycine / Q000031:analogs & derivatives; D006540:Herbicides; D007564:Japan; D007753:Laboratories; D030821:Plants, Genetically Modified / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D013025:Glycine max / Q000235:genetics*; D000097797:Glyphosate
chemical_list: D018744:DNA, Plant; D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate