title: In situ microbial fuel cell-based biosensor for organic carbon.
abstract: The biological oxygen demand (BOD) may be the most used test to assess the amount of pollutant organic matter in water; however, it is time and labor consuming, and is done ex-situ. A BOD biosensor based on the microbial fuel cell principle was tested for online and in situ monitoring of biodegradable organic content of domestic wastewater. A stable current density of 282±23mA/m(2) was obtained with domestic wastewater containing a BOD(5) of 317±15mg O(2)/L at 22±2°C, 1.53±0.04mS/cm and pH 6.9±0.1. The current density showed a linear relationship with BOD(5) concentration ranging from 17±0.5mg O(2)/L to 78±7.6mg O(2)/L. The current generation from the BOD biosensor was dependent on the measurement conditions such as temperature, conductivity, and pH. Thus, a correction factor should be applied to measurements done under different environmental conditions from the ones used in the calibration. These results provide useful information for the development of a biosensor for real-time in situ monitoring of wastewater quality.
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors.forename: Luciana
authors.initials: L
authors.lastname: Peixoto
authors.forename: Booki
authors.initials: B
authors.lastname: Min
authors.forename: Gilberto
authors.initials: G
authors.lastname: Martins
authors.forename: António G
authors.initials: AG
authors.lastname: Brito
authors.forename: Pablo
authors.initials: P
authors.lastname: Kroff
authors.forename: Pier
authors.initials: P
authors.lastname: Parpot
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
authors.forename: Regina
authors.initials: R
authors.lastname: Nogueira
mesh_terms: D001674:Bioelectric Energy Sources; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis / Q000379:methods*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D004553:Electric Conductivity; D004566:Electrodes; D004784:Environmental Monitoring; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014873:Water Pollutants / Q000032:analysis* / Q000737:chemistry
chemical_list: D014873:Water Pollutants; D002244:Carbon