title: Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells.
abstract: OBJECTIVE
  Tremendous efforts have been made to establish effective therapeutic neovascularization using adipose tissue-derived stromal vascular fraction (SVF), but the efficiency is low, and underlying mechanisms and their interaction with the host in a new microenvironment are poorly understood.
  
  
  RESULTS
  Here we demonstrate that direct implantation of SVF derived from donor adipose tissue can create a profound vascular network through the disassembly and reassembly of blood endothelial cells at the site of implantation. This neovasculature successfully established connection with recipient blood vessels to form a functionally perfused circuit. Addition of vascular growth factors to the SVF implant improved the efficiency of functional neovasculature formation. In contrast, spheroid culture of SVF before implantation reduced the capacity of vasculature formation, possibly because of cellular alteration. Implanting SVF into the mouse ischemic hindlimb induced the robust formation of a local neovascular network and salvaged the limb. Moreover, the coimplantation of SVF prevented fat absorption in the subcutaneous adipose tissue graft model.
  
  
  CONCLUSIONS
  Freshly isolated SVF can effectively induce new vessel formation through the dynamic reassembly of blood endothelial cells and could be applied to achieve therapeutic neovascularization for relieving ischemia and preventing fat absorption in an autologous manner.
authors.affiliation: Graduate School of Biomedical Science and Engineering, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Daejeon 305-701, Republic of Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Koh
authors.forename: Bong Ihn
authors.initials: BI
authors.lastname: Koh
authors.forename: Honsoul
authors.initials: H
authors.lastname: Kim
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Joo
authors.forename: Ho Kyoung
authors.initials: HK
authors.lastname: Jin
authors.forename: Jongwook
authors.initials: J
authors.lastname: Jeon
authors.forename: Chulhee
authors.initials: C
authors.lastname: Choi
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
authors.forename: Chung-Hyun
authors.initials: CH
authors.lastname: Cho
authors.forename: Won Seok
authors.initials: WS
authors.lastname: Park
authors.forename: Ji-Kan
authors.initials: JK
authors.lastname: Ryu
authors.forename: Jun Kyu
authors.initials: JK
authors.lastname: Suh
authors.forename: Gou Young
authors.initials: GY
authors.lastname: Koh
mesh_terms: D000042:Absorption; D000273:Adipose Tissue / Q000098:blood supply* / Q000378:metabolism; D042501:Angiogenic Proteins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004338:Drug Combinations; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006614:Hindlimb; D007511:Ischemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D007797:Laminin / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000098:blood supply*; D018919:Neovascularization, Physiologic; D011509:Proteoglycans / Q000378:metabolism; D012039:Regional Blood Flow; D018874:Spheroids, Cellular; D017154:Stromal Cells / Q000378:metabolism / Q000637:transplantation*; D013997:Time Factors
chemical_list: D042501:Angiogenic Proteins; D004338:Drug Combinations; D007797:Laminin; D008164:Luminescent Proteins; D011509:Proteoglycans; C413663:fluorescent protein 583; C059485:matrigel; D049452:Green Fluorescent Proteins; D003094:Collagen