title: Ergosta-7,22-diene-2β,3α,9α-triol from the fruit bodies of Ganoderma lucidum induces apoptosis in human myelocytic HL-60 cells.
abstract: Ganoderma lucidum is known as a medicinal mushroom used in traditional medicine. In our study, the cytotoxic activities of 17 compounds (1-17) isolated from the fruiting bodies of G. lucidum were investigated. Among them, ergosta-7,22-diene-2β,3α,9α-triol (EGDT) induced apoptosis in HL-60 human premyelocytic leukemia cells. EGDT activated the apoptotic process, including DNA fragmentation and caspase-3 activity. In immunoblotting analysis, treatment with EGDT resulted in the cleavage of procaspase-3 and poly(ADP-ribose) polymerase (PARP) into active forms. In the in vivo study, the administration (i.p.) of EGDT to Lewis lung carcinoma (LLC)-inoculated mice evidenced a significant inhibition of tumor growth. These results indicate that EGDT was one of the apoptotic constituents of G. lucidum, and might be an antitumor agent.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Korea.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Lee
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.forename: To Dao
authors.initials: TD
authors.lastname: Cuong
authors.forename: Minkyun
authors.initials: M
authors.lastname: Na
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Kim
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Kim
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Park
authors.forename: Jae Sue
authors.initials: JS
authors.lastname: Choi
authors.forename: Iksoo
authors.initials: I
authors.lastname: Lee
authors.forename: Kihwan
authors.initials: K
authors.lastname: Bae
authors.forename: Masao
authors.initials: M
authors.lastname: Hattori
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy; D053148:Caspase 3 / Q000378:metabolism; D053938:DNA Fragmentation; D005260:Female; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D051379:Mice; D010840:Phytosterols / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010840:Phytosterols; C006954:cerevisterol; C505407:CASP3 protein, human; D053148:Caspase 3