title: The mechanism of linkage-specific ubiquitin chain elongation by a single-subunit E2.
abstract: Ubiquitin chains of different topologies trigger distinct functional consequences, including protein degradation and reorganization of complexes. The assembly of most ubiquitin chains is promoted by E2s, yet how these enzymes achieve linkage specificity is poorly understood. We have discovered that the K11-specific Ube2S orients the donor ubiquitin through an essential noncovalent interaction that occurs in addition to the thioester bond at the E2 active site. The E2-donor ubiquitin complex transiently recognizes the acceptor ubiquitin, primarily through electrostatic interactions. The recognition of the acceptor ubiquitin surface around Lys11, but not around other lysines, generates a catalytically competent active site, which is composed of residues of both Ube2S and ubiquitin. Our studies suggest that monomeric E2s promote linkage-specific ubiquitin chain formation through substrate-assisted catalysis.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Wickliffe
authors.forename: Sonja
authors.initials: S
authors.lastname: Lorenz
authors.forename: David E
authors.initials: DE
authors.lastname: Wemmer
authors.forename: John
authors.initials: J
authors.lastname: Kuriyan
authors.forename: Michael
authors.initials: M
authors.lastname: Rape
mesh_terms: D002384:Catalysis; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D044763:Ubiquitin-Conjugating Enzymes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D025801:Ubiquitin; C544962:Ube2S protein, human; D044763:Ubiquitin-Conjugating Enzymes