title: Sensitive detection of p53 tumor suppressor gene using an enzyme-based solid-state electrochemiluminescence sensing platform.
abstract: An enzyme-based solid-state electrochemiluminescence (ECL) sensing platform for sensitive detection of a single point mutation is developed successfully using p53 tumor suppressor gene as a model analyte. A composite of multiwalled carbon nanotubes and Ruthenium (II) tris-(bipyridine) (MWNTs-Ru(bpy)(3)(2+)) was prepared and coated on an electrode surface, which was covered by polypyrrole (PPy) to immobilize ssDNA. Then, the ssDNA recognized the gold nanoparticle (AuNP)-labeled p53 tumor suppressor gene, and produced AuNP-dsDNA electrode with AuNP layer. The surface adsorbed the glucose-dehydrogenase (GDH) molecules for producing ECL signal. This system combined enzyme reaction with ECL detection, and it can recognize sequence-specific wild type p53 sequence (wtp53) and muted type p53 sequence (mtp53) with discrimination of up to 56.3%. The analytic results were sensitive and specific. It holds promise for the diagnosis and management of cancer.
authors.affiliation: Department of Hygienic Analytical Chemistry, Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Zhang
authors.forename: Pingang
authors.initials: P
authors.lastname: He
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Fang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D004563:Electrochemistry; D004566:Electrodes; D016158:Genes, p53; D008163:Luminescent Measurements / Q000295:instrumentation*; D037742:Nanotubes, Carbon / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D037742:Nanotubes, Carbon