title: Radiolabeling of DNA using polynucleotide kinase.
abstract: Labeling of DNA fragments at the 5' end with polynucleotide kinase is one of the methods currently employed to obtain highly labeled DNA for sequencing purposes. This end-labeled DNA is used in the partial chemical degradation method for sequencing DNA (see Chapter 51 and ref. 1).
authors.affiliation: Department of Microbiology, The Technical University of Denmark, Lyngby, Denmark.
authors.forename: W
authors.initials: W
authors.lastname: Gaastra
authors.forename: J
authors.initials: J
authors.lastname: Josephsen