title: Effect of Trichosanthes dioica on oxidative stress and CYP450 gene expression levels in experimentally induced diabetic rats.
abstract: The present study is a systematic and scientific evaluation of antioxidant potential of aqueous extract of Trichosanthes dioica fruits on diabetes induced oxidative stress of severely diabetic rats. Its effect on gene expression level of the tissue—specific cytochrome P450 (CYP) was also studied. The dose of 1000mg/kg bw of T. dioica extract was administered orally once a day for 28 days to severely diabetic rats. Various oxidative stress parameters were analyzed in selected tissues of control as well as treated diabetic rats. The elevated level of lipid peroxidation was decreased and the decreased activities of antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase and glutathione-S-transferase were increased significantly (p<0.05) in treated rats. Diabetes induced increased expression levels of drug metabolizing gene viz CYP 450 were drastically inhibited after the treatment. Thus the inhibition of gene expression level can be correlated with the exogenous metabolism and hence it effectiveness.
authors.affiliation: University of Allahabad, Department of Chemistry Alternative Therapeutics Unit, Drug Discovery & Development Division, Medicinal Research Lab, Allahabad, India.
authors.forename: P
authors.initials: P
authors.lastname: Kumar Rai
authors.forename: D
authors.initials: D
authors.lastname: Kumar Rai
authors.forename: S
authors.initials: S
authors.lastname: Mehta
authors.forename: R
authors.initials: R
authors.lastname: Gupta
authors.forename: B
authors.initials: B
authors.lastname: Sharma
authors.forename: G
authors.initials: G
authors.lastname: Watal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005638:Fruit / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D029778:Trichosanthes / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D003577:Cytochrome P-450 Enzyme System; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione