title: Allitridi inhibits transient outward potassium currents in human atrial myocytes.
abstract: 1. It has been reported that allitridi, an active compound extracted from garlic, has many cardiovascular effects. However, it remains unknown whether allitridi affects major repolarization currents, such as the transient outward K(+) current (I(to) ), ultrarapid delayed rectifier K(+) current (I(Kur)) and the L-type Ca(2+) current (I(Ca)), in human atrial myocytes. 2. In the present study, we investigated the effects of allitridi on I(to), I(Kur), I(Ca) and the action potential in human isolated atrial myocytes using the whole-cell patch recording technique. 3. Allitridi reversibly inhibited I(to), but not I(Kur) and I(Ca), in human atrial myocytes. These effects of allitridi on I(to) were concentration dependent (IC(50) = 44.9 μmol/L). Inactivation of I(to) was accelerated and the voltage-dependent inactivation potential was shifted towards the negative direction. Allitridi (30 μmol/L) significantly prolonged action potential duration in human atrial myocytes. 4. The results of the present study indicate that allitridi inhibits I(to), but not I(Kur) and I(Ca), and prolongs the action potential duration in human atrial myocytes.
authors.affiliation: Department of Cardiology, Guangdong Cardiovascular Institute and Guangdong Academy of Medical Sciences, Research Centre of Guangdong General Hospital, Guangzhou, China.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Deng
authors.forename: Fang
authors.initials: F
authors.lastname: Rao
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Kuang
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Wu
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Shan
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhou
authors.forename: Qiu-Xiong
authors.initials: QX
authors.lastname: Lin
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Lin
authors.forename: Xi-Yong
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000328:Adult; D000368:Aged; D000498:Allyl Compounds / Q000494:pharmacology*; D016275:Atrial Function / Q000187:drug effects; D002317:Cardiovascular Agents / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004594:Electrophysiology; D006325:Heart Atria / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008875:Middle Aged; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D002317:Cardiovascular Agents; D015221:Potassium Channels; D013440:Sulfides; C042577:diallyl trisulfide