title: Repression of porcine endogenous retrovirus infection by human APOBEC3 proteins.
abstract: It has been shown that porcine endogenous retrovirus (PERV) can infect human cells, indicating that PERV transmission poses a serious concern in pig-to-human xenotransplantation. A number of recent studies have reported on retrovirus interference by antiviral proteins. The most potent antiviral proteins are members of the APOBEC family of cytidine deaminases, which are involved in defense against retroviral attack. These proteins are present in the cytoplasm of mammalian cells and inhibit retroviral replication. To evaluate the inhibition of PERV transmission by human APOBEC3 proteins, we co-transfected 293T cells with a PERV molecular clone and human APOBEC3F or APOBEC3G expression vectors, and monitored PERV replication competency using a quantitative analysis of PERV pol genes. The replication of PERVs in cells co-expressing human APOBEC3s was reduced by 60-90% compared with PERV-only control. These results suggest that human APOBEC3G and APOBEC3F might serve a potential barrier function against PERV transmission in xenotransplantation.
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, South Korea. eurofa@konkuk.ac.kr
authors.forename: Jungeun
authors.initials: J
authors.lastname: Lee
authors.forename: Jae Yoo
authors.initials: JY
authors.lastname: Choi
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Kang-Chang
authors.initials: KC
authors.lastname: Kim
authors.forename: Byeong-Sun
authors.initials: BS
authors.lastname: Choi
authors.forename: Yu-Kyoung
authors.initials: YK
authors.lastname: Oh
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
mesh_terms: D000071478:APOBEC Deaminases; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003564:Cytidine Deaminase; D043525:Cytosine Deaminase / Q000235:genetics*; D020077:Endogenous Retroviruses / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D012192:Retroviridae Infections / Q000235:genetics* / Q000635:transmission*; D013552:Swine / Q000821:virology*; D014183:Transplantation, Heterologous / Q000009:adverse effects; D014779:Virus Replication
chemical_list: D043525:Cytosine Deaminase; D000071478:APOBEC Deaminases; C502889:APOBEC3 proteins, human; D003564:Cytidine Deaminase