title: [Screening of yeast-producers of alpha-L-rhamnosidase].
abstract: Screening of producers of alpha-L-rhamnosidase among 331 yeast strains (representatives of 12 genera and 17 species) has been performed. A capacity to synthesize the enzyme was revealed in 45% of the studied strains with activity 0.01-0.6 un/ml. Complex enzymatic preparations of alpha-L-rhamnosidase were obtained from cultural liquid of two the most active producers by fractionation with ammonium sulfate (90% saturation). The influence of pH and temperature on enzymes activities and also specificity towards natural and synthetic substrates were studied.
authors.forename: O M
authors.initials: OM
authors.lastname: Rzaieva
authors.forename: L D
authors.initials: LD
authors.lastname: Varbanets'
authors.forename: S S
authors.initials: SS
authors.lastname: Nahorna
mesh_terms: D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*
chemical_list: D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase