title: Synthesis of potential metal-binding group compounds to examine the zinc dependency of the GPI de-N-acetylase metalloenzyme in Trypanosoma brucei.
abstract: A small zinc-binding group (ZBG) library of deoxy-2-C-branched-monosaccharides, for example, 1,5-anhydroglucitols, consisting of either monodentate ligand binding carboxylic acids or bidentate ligand binding hydroxamic acids, were prepared to assess the zinc affinity of the putative metalloenzyme 2-acetamido-2-deoxy-α-D-glucopyranosyl-(1→6)-phosphatidylinositol de-N-acetylase (EC 3.5.1.89) of glycosylphosphatidylinositol biosynthesis. The N-ureido thioglucoside was also synthesised and added to the ZBG library because a previous N-ureido analogue, synthesised by us, had inhibitory activity against the aforementioned de-N-acetylase, presumably via the N-ureido motif.
authors.affiliation: Division of Biological Chemistry and Drug Discovery, College of Life Sciences, The University of Dundee, DD1 5EH Dundee, Scotland, United Kingdom.
authors.forename: Nuha Z
authors.initials: NZ
authors.lastname: Abdelwahab
authors.forename: Michael D
authors.initials: MD
authors.lastname: Urbaniak
authors.forename: Michael A J
authors.initials: MA
authors.lastname: Ferguson
authors.forename: Arthur T
authors.initials: AT
authors.lastname: Crossman
mesh_terms: D000115:Acetylesterase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis; D009682:Magnetic Resonance Spectroscopy; D008667:Metalloproteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015394:Molecular Structure; D014346:Trypanosoma brucei brucei / Q000201:enzymology*; D015032:Zinc / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D017261:Glycosylphosphatidylinositols; D008667:Metalloproteins; D000115:Acetylesterase; D015032:Zinc