title: The anti-angiogenic peptide anginex greatly enhances galectin-1 binding affinity for glycoproteins.
abstract: Angiogenesis is a key event in cancer progression and therefore a promising target in cancer treatment. Galectin-1, a β-galactoside binding lectin, is up-regulated in the endothelium of tumors of different origin and has been shown to be the target for anginex, a powerful anti-angiogenic peptide with anti-tumor activity. Here we show that when bound to anginex, galectin-1 binds various glycoproteins with hundred- to thousand-fold higher affinity. Anginex also interacts with galectin-2, -7, -8N, and -9N but not with galectin-3, -4, or -9C.
authors.affiliation: Section Microbiology, Immunology, Glycobiology, Institute of Laboratory Medicine, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Salomonsson
authors.forename: Victor L
authors.initials: VL
authors.lastname: Thijssen
authors.forename: Arjan W
authors.initials: AW
authors.lastname: Griffioen
authors.forename: Ulf J
authors.initials: UJ
authors.lastname: Nilsson
authors.forename: Hakon
authors.initials: H
authors.lastname: Leffler
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004187:Disaccharides / Q000737:chemistry; D005454:Fluorescence Polarization / Q000379:methods; D037483:Galectin 1 / Q000096:biosynthesis*; D037161:Galectins / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D006242:Haptoglobins / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D009369:Neoplasms / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D000509:alpha-Fetoproteins / Q000737:chemistry
chemical_list: D020533:Angiogenesis Inhibitors; D004187:Disaccharides; D037483:Galectin 1; D037161:Galectins; D006001:Glycoconjugates; D006023:Glycoproteins; D006242:Haptoglobins; D037102:Lectins; D010455:Peptides; D000509:alpha-Fetoproteins; C558923:anginex peptide