title: The osteogenetic effect of astragaloside IV with centrifugating pressure on the OCT-1 cells.
abstract: Astragaloside IV (ASI), a pure compound derived from Radix Astragali, is commonly used in degenerative bone diseases such as osteoporosis. Our previous study identified in vivo the osteogenetic effect of Fu Fang Qi She Pills (FFQSP), a Chinese herbal formula containing Radix Astragali from which ASI was extracted. In this study, we investigated the osteogenetic effects of ASI under the conditions of centrifugating pressure on OCT-1 cells. These preosteoblasts were grown in 3D-culture, and treated with ASI at 50 micromol/l with centrifugation at 200 rpm, 500 rpm for 3 and 5 days. Morphocytological examination, morphometry of alkaline phosphatases (ALP) staining was performed. Expression of type I collagen (Col I) was detected by immunocytochemistry assays. ALP, Col1a2, Osteocalcin (OC), and runt-related transcription factor-2 (Runx2) mRNA expression were determined via real-time PCR. The results showed ASI plus 500 rpm for 3 days and ASI plus 200 rpm for 5 days significantly induced osteogenesis related protein and gene expression. We concluded that ASI would promote osteogenesis when cells of preosteoblast OCT-1 were subjected to proper centrifugating pressure and a pertinent period of time.
authors.affiliation: Department of Orthopaedics and Traumatology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Bian
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Huang
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Liang
authors.forename: Bing
authors.initials: B
authors.lastname: Shu
authors.forename: Wei
authors.initials: W
authors.lastname: Hou
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Sheng
authors.initials: S
authors.lastname: Lu
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002498:Centrifugation; D024042:Collagen Type I / Q000096:biosynthesis; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects*; D011187:Posture; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D024042:Collagen Type I; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; C493717:Runx2 protein, rat; D012503:Saponins; D013778:Tetrazolium Salts; D013844:Thiazoles; D014315:Triterpenes; D015675:Osteocalcin; C052064:astragaloside A; D012313:RNA; D000469:Alkaline Phosphatase; C022616:thiazolyl blue