title: Relative and combined effects of selenium, protein deficiency and ethanol on bone.
abstract: Some observations suggest that oxidative damage may affect both osteoblastic function and osteoclastic activity in alcohol-mediated bone alterations. Selenium, a potent antioxidant, is decreased in alcoholics.
  
  
  OBJECTIVE
  To analyse if the supplementation with selenium may alter bone changes observed in a murine model fed ethanol and/or a 2% protein-containing diet, following the Lieber-deCarli design.
  
  
  METHODS
  Adult male Sprague-Dawley rats were divided into 8 groups, which received the Lieber-DeCarli control diet, an isocaloric, 36% ethanol-containing diet, an isocaloric, 2% protein-containing diet; and an isocaloric diet containing 2% protein and 36% ethanol diet, and another similar four groups to which selenomethionine (1mg/kg body weight). After sacrifice (5 weeks later), trabecular bone mass was histomorphometrically assessed, bone and serum selenium were determined by flame atomic absorption spectrophotometry, and serum osteocalcin, insulin growth factor 1 (IGF-1), PTH and telopeptide, by radioimmunoanalysis. Liver glutathione peroxidase (GPX) activity was also determined.
  
  
  RESULTS
  Ethanol-fed rats showed decreased TBM, IGF-1 and osteocalcin, especially when ethanol was added to a 2%-protein diet. Selenium did not modify at all bone parameters, despite a marked increase in serum selenium and a less pronounced one in bone selenium, and an increase in liver GPX.
  
  
  CONCLUSIONS
  Our results do not support the existence of a beneficial effect of selenium addition on bone changes observed in this murine model treated following the Lieber-deCarli experimental design.
authors.affiliation: Servicio de Medicina Interna, Hospital Universitario de Canarias, Tenerife-Canary Islands, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: González-Pérez
authors.forename: Emilio
authors.initials: E
authors.lastname: González-Reimers
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Durán-Castellón
authors.forename: Franscisco
authors.initials: F
authors.lastname: Santolaria-Fernández
authors.forename: Luis
authors.initials: L
authors.lastname: Galindo-Martín
authors.forename: Rosa
authors.initials: R
authors.lastname: RosVilamajó
authors.forename: María José
authors.initials: MJ
authors.lastname: de la Vega-Prieto
authors.forename: Jose
authors.initials: J
authors.lastname: Viña-Rodríguez
authors.forename: Pedro
authors.initials: P
authors.lastname: Abreu-González
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D004032:Diet; D019587:Dietary Supplements; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D011488:Protein Deficiency / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000008:administration & dosage / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D014131:Trace Elements / Q000032:analysis
chemical_list: D012997:Solvents; D014131:Trace Elements; D000431:Ethanol; D012643:Selenium