title: Structural bases of physiological functions and roles of the vacuolar H(+)-ATPase.
abstract: Vacuolar-type H(+)-ATPases (V-ATPases) is a large multi-protein complex containing at least 14 different subunits, in which subunits A, B, C, D, E, F, G, and H compose the peripheral 500-kDa V(1) responsible for ATP hydrolysis, and subunits a, c, c', c″, and d assembly the 250-kDa membrane-integral V(0) harboring the rotary mechanism to transport protons across the membrane. The assembly of V-ATPases requires the presence of all V(1) and V(0) subunits, in which the V(1) must be completely assembled prior to association with the V(0), accordingly the V(0) failing to assemble cannot provide a membrane anchor for the V(1), thereby prohibiting membrane association of the V-ATPase subunits. The V-ATPase mediates acidification of intracellular compartments and regulates diverse critical physiological processes of cell for functions of its numerous functional subunits. The core catalytic mechanism of the V-ATPase is a rotational catalytic mechanism. The V-ATPase holoenzyme activity is regulated by the reversible assembly/disassembly of the V(1) and V(0), the targeting and recycling of V-ATPase-containing vesicles to and from the plasma membrane, the coupling ratio between ATP hydrolysis and proton pumping, ATP, Ca(2+), and its inhibitors and activators.
authors.affiliation: Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China.
authors.forename: Binyun
authors.initials: B
authors.lastname: Ma
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiang
authors.forename: Lizhe
authors.initials: L
authors.lastname: An
mesh_terms: D000199:Actins / Q000378:metabolism / Q000502:physiology; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D025262:Vacuolar Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology
chemical_list: D000199:Actins; D004791:Enzyme Inhibitors; D021122:Protein Subunits; D025262:Vacuolar Proton-Translocating ATPases