title: Antimicrobial effect of Magnolia officinalis extract against Staphylococcus aureus.
abstract: BACKGROUND
  The antimicrobial effect of Magnolia officinalis extract (MOE) against Staphylococcus aureus was investigated in a minced mutton system and the mechanisms of its antimicrobial activity were studied by light microscopy, transmission electron microscopy and scanning electron microscopy observations.
  
  
  RESULTS
  MOE inoculation effectively inhibited the growth of S. aureus in minced mutton compared with that in control meat without MOE. The cell membrane of S. aureus treated with MOE showed structural disorganisation and cytoplasmic volume overflow. After 48 h of exposure to MOE, many S. aureus cells had completely collapsed.
  
  
  CONCLUSIONS
  The antimicrobial mechanisms of MOE resulted mainly in cell membrane and wall damage, causing increased permeability of cell membranes or lysis of cell walls and loss of cellular constituents, impairment of structural components and changes in bacterial cell morphology.
authors.affiliation: School of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Hu
authors.forename: Jinling
authors.initials: J
authors.lastname: Qiao
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Changrong
authors.initials: C
authors.lastname: Ge
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001433:Bacteriolysis / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000648:ultrastructure; D002681:China; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005520:Food Preservatives / Q000302:isolation & purification / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry*; D008461:Meat Products / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D034561:Sheep, Domestic; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D005520:Food Preservatives; D010936:Plant Extracts