title: [Simultaneous determination of six synthetic sweeteners in food by high performance liquid chromatography-tandem mass spectrometry].
abstract: A simple and sensitive method for the determination of six synthetic sweeteners (sodium cyclamate, saccharin sodium, acesulfame-K, aspartame, alitame and neotame) in food was developed. The synthetic sweeteners were extracted by methanol-water (1 : 1, v/v). The extract was separated on a C18 column using 0.1% (v/v) formic acid-5 mmol/L ammonium formate/acetonitrile as mobile phase, and then detected by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using multiple reaction monitoring (MRM) mode. The good linearities (r > 0.998) were achieved for all the analytes over the range of 20-500 microg/L. The recoveries obtained ranged from 81.3% to 106.0% at three spiked concentrations, with the relative standard deviations lower than 11%. The established method has been successfully applied to the determination of synthetic sweeteners in food.
authors.affiliation: Hunan Academy of Inspection and Quarantine, Technical Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Hunan Key Laboratory of Food Safety Science & Technology, Changsha 410004, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Jinxia
authors.initials: J
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Huang
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003494:Cyclamates / Q000032:analysis; D005503:Food Additives / Q000032:analysis*; D012439:Saccharin / Q000032:analysis; D013549:Sweetening Agents / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013843:Thiazines / Q000032:analysis
chemical_list: D003494:Cyclamates; D005503:Food Additives; D013549:Sweetening Agents; D013843:Thiazines; D012439:Saccharin; C006362:acetosulfame