title: [Immunobiologic characteristics of carbohydrate-containing preparations of Haemophilus influenzae].
abstract: OBJECTIVE
  Comparative assessment of immunobiological characteristics of 3 antigenic preparations containing capsular polysaccharide of Haemophilus influenzae type b (CPS Hib).
  
  
  METHODS
  The following preparations were assessed: CPS Hib obtained by using cetavlon; hydroxylamine preparation of Hib (HAP Hib); mixture of CPS Hib and lipooligosaccharide of non-typeable H. influenzae (LOS NTHi) detoxified by hydroxylamine hydrochloride. Effects of these preparations on immunophenotype of mononuclear leukocytes of mice spleen as well as on spectrum and level of cytokines in serum were studied.
  
  
  RESULTS
  It was shown that mixture of CPS Hib and detoxified LOS NTHi has low toxicity and most protective activity during Hib challenge leading to activation of innate immunity effectors and initiation of adaptive immune response.
  
  
  CONCLUSIONS
  Obtained data provide perspective for development of preparation able to protect from infections caused by both capsular and acapsular strains of H. influenzae.
authors.forename: O V
authors.initials: OV
authors.lastname: Novikova
authors.forename: N G
authors.initials: NG
authors.lastname: Kalina
authors.forename: N P
authors.initials: NP
authors.lastname: Vaneeva
authors.forename: S I
authors.initials: SI
authors.lastname: Elkina
authors.forename: M M
authors.initials: MM
authors.lastname: Tokarskaia
authors.forename: N E
authors.initials: NE
authors.lastname: Iastrebova
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D016207:Cytokines / Q000097:blood; D006192:Haemophilus Infections / Q000097:blood / Q000517:prevention & control*; D018073:Haemophilus Vaccines / Q000276:immunology*; D019741:Haemophilus influenzae type b / Q000276:immunology*; D019811:Hydroxylamine / Q000737:chemistry; D007113:Immunity, Innate; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D002593:Cetrimonium Compounds; D016207:Cytokines; D018073:Haemophilus Vaccines; D019811:Hydroxylamine; D000077286:Cetrimonium