title: Chronic nicotine exposure attenuates proangiogenic activity on human umbilical vein endothelial cells.
abstract: The pathogenic mechanism of nicotine, a major product of smoking, on vascular endothelial cells is not well defined yet. The purpose of this study was to determine whether chronic exposure to nicotine alters angiogenic activity in human umbilical vein endothelial cells and to identify a potential role for endothelial nitric oxide synthase (eNOS) expression. Our study demonstrated that acute nicotine treatment enhanced nitric oxide release, eNOS activation, and proangiogenic activity. However, chronic nicotine exposure impaired proangiogenic function (decreased cell migration and tubular structure formation) in human umbilical vein endothelial cells compared with acute exposure, but sustained the antiapoptotic effect. These findings seem to be related to eNOS gene expression and nitric oxide production, which may be involved in the pathophysiology of chronic nicotine addicts.
authors.affiliation: Department of Surgery, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hyung Sub
authors.initials: HS
authors.lastname: Park
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Cho
authors.forename: Yang Jin
authors.initials: YJ
authors.lastname: Park
authors.forename: Taeseung
authors.initials: T
authors.lastname: Lee
mesh_terms: D043924:Angiogenesis Modulating Agents / Q000008:administration & dosage / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D054443:Cell Migration Assays; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009538:Nicotine / Q000008:administration & dosage / Q000009:adverse effects*; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D052250:Nitric Oxide Synthase Type III / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012906:Smoke; D014026:Nicotiana; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: D043924:Angiogenesis Modulating Agents; D012906:Smoke; D009569:Nitric Oxide; D009538:Nicotine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III