title: Whole-cell recording of calcium release-activated calcium (CRAC) currents in human T lymphocytes.
abstract: In T lymphocytes, depletion of Ca(2+) from the intracellular Ca(2+) store leads to activation of plasmalemmal Ca(2+) channels, called Calcium Release-Activated Calcium (CRAC) channels. CRAC channels play important role in regulation of T cell proliferation and gene expression. Abnormal CRAC channel function in T cells has been linked to severe combined immunodeficiency and autoimmune diseases. Studying CRAC channel function in human T cells may uncover new molecular mechanisms regulating normal immune responses and unravel the causes of related human diseases. Electrophysiological recordings of membrane currents provide the most accurate assessment of functional channel properties and their regulation. Electrophysiological assessment of CRAC channel currents in Jurkat T cells, a human leukemia T cell line, was first performed more than 20 years ago, however, CRAC current measurements in normal human T cells remains a challenging task. The difficulties in recording CRAC channel currents in normal T cells are compounded by the fact that blood-derived T lymphocytes are much smaller in size than Jurkat T cells and, therefore, the endogenous whole-cell CRAC currents are very low in amplitude. Here, we give a step-by-step procedure that we routinely use to record the Ca(2+) or Na(+) currents via CRAC channels in resting human T cells isolated from the peripheral blood of healthy volunteers. The method described here was adopted from the procedures used for recording the CRAC currents in Jurkat T cells and activated human T cells.
authors.affiliation: Department of Physiology and Membrance Biology, University of California, Davis, USA.
authors.forename: Pratima
authors.initials: P
authors.lastname: Thakur
authors.forename: Alla F
authors.initials: AF
authors.lastname: Fomina
mesh_terms: D015220:Calcium Channels / Q000502:physiology*; D055724:Electrophysiological Phenomena; D006801:Humans; D019169:Jurkat Cells; D018408:Patch-Clamp Techniques / Q000379:methods*; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D015220:Calcium Channels