title: Liver regeneration after partial hepatectomy in rat is more impaired in a steatotic liver induced by dietary fructose compared to dietary fat.
abstract: Hepatic steatosis (HS) has a negative effect on liver regeneration, but different pathophysiologies of HS may lead to different outcomes. Male Sprague-Dawley rats were fed a high fructose (66% fructose; H-fruc), high fat (54% fat; H-fat), or control chow diet for 4 weeks. Based on hepatic triglyceride content and oil red O staining, HS developed in the H-fruc group, but was less severe compared to the H-fat group. Hepatic mRNA expression levels of fatty acid synthase and fructokinase were increased and those of carnitine palmitoyltransferase-1 and peroxisome proliferator-activated receptor-α were decreased in the H-fruc group compared to the H-fat group. Liver regeneration after 70% partial hepatectomy (PHx) was evaluated by measuring the increase in postoperative liver mass and PCNA-positive hepatocytes, and was impaired in the H-fruc group compared to the H-fat and control groups on days 3 and 7. Serum levels of tumor necrosis factor-α, interleukin-6 and hepatocyte growth factor did not change significantly after PHx. In contrast, serum TGF-β1 levels were slightly but significantly lower in the control group on day 1 and in the H-fat group on day 3 compared to the level in each group on day 0, and then gradually increased. However, the serum TGF-β1 level did not change after PHx in the H-fruc group. These results indicate that impairment of liver regeneration after PHx in HS is related to the cause, rather than the degree, of steatosis. This difference may result from altered metabolic gene expression profiles and potential dysregulation of TGF-β1 expression.
authors.affiliation: Department of Digestive and Lifestyle-Related Diseases, Kagoshima University, Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Shirou
authors.initials: S
authors.lastname: Tanoue
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Uto
authors.forename: Ryo
authors.initials: R
authors.lastname: Kumamoto
authors.forename: Shiho
authors.initials: S
authors.lastname: Arima
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Nasu
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Takami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Moriuchi
authors.forename: Toshio
authors.initials: T
authors.lastname: Sakiyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Oketani
authors.forename: Akio
authors.initials: A
authors.lastname: Ido
authors.forename: Hirohito
authors.initials: H
authors.lastname: Tsubouchi
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage / Q000009:adverse effects*; D005234:Fatty Liver / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D005632:Fructose / Q000008:administration & dosage / Q000009:adverse effects*; D006498:Hepatectomy; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D004041:Dietary Fats; D005632:Fructose