title: Isolation of mouse thymic dendritic cells.
abstract: The method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (1,2) and involves the following major steps: 1. Enzymatic digestion of thymic fragments with collagenase and DNase. 2. Separation of a very-low-density cell fraction (VLDF). 3. Magnetic depletion of T-lineage cells, B cells, macrophages, and granulocytes. 4. Positive selection of DCs by magnetic cell sorting (MACS).
authors.affiliation: Department of Cell Biology, Complutense University, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Anjuère
authors.forename: C
authors.initials: C
authors.lastname: Ardavín