title: Bioimpedance spectroscopy: a new tool to assess early esophageal changes linked to gastroesophageal reflux disease?
abstract: Bioimpedance spectroscopy can identify pathological changes related to precancerous lesions of the cervix uteri and esophagus. It therefore has the potential to detect early reflux-related changes in the esophageal mucosa, such as dilated intercellular spaces. The reliable detection of dilated intercellular spaces at the time of endoscopy would yield a significant diagnostic advantage for separating patients with functional heartburn from the large proportion of patients with gastroesophageal reflux symptoms but no macroscopic esophagitis or pathological acid exposure. The bioimpedance of the esophageal mucosa, measured with a small caliber probe, was evaluated in a series of preclinical experiments. First, sections of rabbit esophageal epithelium were mounted in Ussing chambers and exposed to solutions at pH 7.4 or pH 1.5 for 45 minutes. Impedance measurements were taken at varying probe pressures. Second, rabbit esophageal epithelia were perfused for 45 minutes in situ with pH 1.1 or control solutions and impedance measurements taken. Samples from both in vitro and in situ experiments were taken for morphological examination by light microscopy. Finally, esophageal bioimpedance was measured in awake dogs with permanent esophagocutaneous stoma. The in situ experiments demonstrated that morphological changes in the esophageal mucosa could be discerned by the use of bioimpedance spectroscopy. The variability in resistivity was species-independent but was affected by the pressure applied to the probe. The results suggest that evaluation of bioimpedance spectroscopy for use in a clinical setting is warranted. Small morphological differences in the esophageal mucosa may be detected by the use of bioimpedance spectroscopy.
authors.affiliation: AstraZeneca Research & Development, Early Clinical Development, Mölndal, Sweden. pal.lundin@astrazeneca.com
authors.forename: P
authors.initials: P
authors.lastname: Lundin
authors.forename: M
authors.initials: M
authors.lastname: Karpefors
authors.forename: K
authors.initials: K
authors.lastname: Carlsson
authors.forename: M B
authors.initials: MB
authors.lastname: Hansen
authors.forename: M
authors.initials: M
authors.lastname: Ruth
mesh_terms: D000818:Animals; D058266:Dielectric Spectroscopy; D004285:Dogs; D042241:Early Diagnosis; D005764:Gastroesophageal Reflux / Q000175:diagnosis*; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits