title: Characterization of the micA gene encoding a small regulatory σE-dependent RNA in Salmonella enterica serovar Typhimurium.
abstract: The role of MicA (repressing small regulatory non-coding RNAs of two Salmonella porins) was determined in virulence of Salmonella enterica serovar Typhimurium. Transcriptional analysis revealed that the expression of the micA gene is driven by a single σ(E)-dependent promoter, micAp. Its activity increased towards stationary phase; in exponential phase, the activity was induced by several stresses by a DegS-dependent mechanism. Although phenotypic analysis revealed no significant differences between wild-type and the micA mutant strains, in vivo studies showed that this mutant is more virulent in the mouse model.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 845 51, Bratislava, Slovak Republic.
authors.forename: D
authors.initials: D
authors.lastname: Homerova
authors.forename: B
authors.initials: B
authors.lastname: Rezuchova
authors.forename: A
authors.initials: A
authors.lastname: Stevenson
authors.forename: H
authors.initials: H
authors.lastname: Skovierova
authors.forename: M
authors.initials: M
authors.lastname: Roberts
authors.forename: J
authors.initials: J
authors.lastname: Kormanec
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D012480:Salmonella Infections / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D022661:RNA, Untranslated; D012808:Sigma Factor