title: Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation.
abstract: mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.
authors.affiliation: Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA. jf2192@columbia.edu
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
authors.forename: James B
authors.initials: JB
authors.lastname: Munro
authors.forename: Scott C
authors.initials: SC
authors.lastname: Blanchard
authors.forename: Joachim
authors.initials: J
authors.lastname: Frank
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010441:Peptide Chain Elongation, Translational / Q000502:physiology; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D012343:RNA, Transfer