title: Effect of light and reductones on differentiation of Pleurotus ostreatus.
abstract: Vegetative mycelia of Pleurotus ostreatus were differentiated into primordia and subsequently into fruit bodies in synthetic sucrose-asparagine medium when exposed to light at low temperature. During photo-morphogenesis, L-ascorbic acid-like substances called reductones were produced. L-ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid and 5-methyl-5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid were accumulated initially in the illuminated mycelia before the initiation of fruiting. The content of glycosides of erythroascorbic acid and their methylated compounds increased again in the primordia and the fruit bodies. Exogenous L-ascorbic acid induced the formation of primordia from the mycelia in the dark in a dose-dependent manner. Thus, this suggests that these reductones might play a role in mediating the light stimulus in photomorphogenesis.
authors.affiliation: Department of Biochemistry, Research Center for Aging and Geriatrics, Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju, 501-190, Republic of Korea. leesr@chonnamac.kr
authors.forename: Seung-Rock
authors.initials: SR
authors.lastname: Lee
authors.forename: Woo-Jeong
authors.initials: WJ
authors.lastname: Joo
authors.forename: Yong-Un
authors.initials: YU
authors.lastname: Baek
authors.forename: Inyoung
authors.initials: I
authors.lastname: Kim
authors.forename: Kee-Oh
authors.initials: KO
authors.lastname: Chay
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Cho
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D008027:Light; D020076:Pleurotus / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects*; D013696:Temperature
chemical_list: D000975:Antioxidants; D003470:Culture Media; D001205:Ascorbic Acid