title: On-line cleanup for 2-aminobenzamide-labeled oligosaccharides.
abstract: Analysis of 2-aminobenzamide-labeled oligosaccharides requires removal of excess labeling reagents before chromatography. Manual cleanup is time-consuming and not optimal for routine analysis, so an on-line solid-phase extraction was developed. Labeled oligosaccharides are trapped on an amide phase in a small guard column, the excess reagents are washed away, and then the sample is transferred to the analytical column for analysis. The on-line protocol shortened the sample preparation time and has been applied for the analysis of oligosaccharides and N-glycans released from glycoproteins.
authors.affiliation: Quality and Safety Department, Nestlé Research Centre, Lausanne, Switzerland.
authors.forename: Thierry
authors.initials: T
authors.lastname: Bénet
authors.forename: Sean
authors.initials: S
authors.lastname: Austin
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis; D052616:Solid Phase Extraction; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D062367:ortho-Aminobenzoates; C000219:anthranilamide