title: Comparison of polymyxin B, tigecycline, cefepime, and meropenem MICs for KPC-producing Klebsiella pneumoniae by broth microdilution, Vitek 2, and Etest.
abstract: We report MIC agreement and error rates between broth microdilution (BMD), Vitek 2, and Etest against 48 clinical KPC-producing Klebsiella pneumoniae isolates for polymyxin B, tigecycline, cefepime, and meropenem. Both commercial testing methods were useful for tigecycline testing; Etest provided a conservative estimate of polymyxin B susceptibility. We suggest that laboratories consider the supplemental use of reference BMD or Etest for cefepime and meropenem for susceptibility testing of KPC-producing K. pneumoniae, as Vitek 2 did not provide reliable results.
authors.affiliation: Department of Pharmacy, New York-Presbyterian Hospital, Columbia University Medical Center, New York, NY 10032, USA. asl9009@nyp.org
authors.forename: Asma
authors.initials: A
authors.lastname: Lat
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Clock
authors.forename: Fann
authors.initials: F
authors.lastname: Wu
authors.forename: Susan
authors.initials: S
authors.lastname: Whittier
authors.forename: Phyllis
authors.initials: P
authors.lastname: Della-Latta
authors.forename: Kathy
authors.initials: K
authors.lastname: Fauntleroy
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Jenkins
authors.forename: Lisa
authors.initials: L
authors.lastname: Saiman
authors.forename: Christine J
authors.initials: CJ
authors.lastname: Kubin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D008911:Minocycline / Q000031:analogs & derivatives* / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology*; D000078304:Tigecycline; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D000078304:Tigecycline; D001618:beta-Lactamases; D008911:Minocycline; D011112:Polymyxin B