title: The ABRF Proteomics Research Group studies: educational exercises for qualitative and quantitative proteomic analyses.
abstract: Resource (core) facilities have played an ever-increasing role in furnishing the scientific community with specialized instrumentation and expertise for proteomics experiments in a cost-effective manner. The Proteomics Research Group (PRG) of the Association of Biomolecular Resource Facilities (ABRF) has sponsored a number of research studies designed to enable participants to try new techniques and assess their capabilities relative to other laboratories analyzing the same samples. Presented here are results from three PRG studies representing different samples that are typically analyzed in a core facility, ranging from simple protein identification to targeted analyses, and include intentional challenges to reflect realistic studies. The PRG2008 study compares different strategies for the qualitative characterization of proteins, particularly the utility of complementary methods for characterizing truncated protein forms. The use of different approaches for determining quantitative differences for several target proteins in human plasma was the focus of the PRG2009 study. The PRG2010 study explored different methods for determining specific constituents while identifying unforeseen problems that could account for unanticipated results associated with the different samples, and included (15) N-labeled proteins as an additional challenge. These studies provide a valuable educational resource to research laboratories and core facilities, as well as a mechanism for establishing good laboratory practices.
authors.affiliation: Vanderbilt University School of Medicine, Nashville, TN, USA. david.friedman@Vanderbilt.edu
authors.forename: David B
authors.initials: DB
authors.lastname: Friedman
authors.forename: Tracy M
authors.initials: TM
authors.lastname: Andacht
authors.forename: Maureen K
authors.initials: MK
authors.lastname: Bunger
authors.forename: Allis S
authors.initials: AS
authors.lastname: Chien
authors.forename: David H
authors.initials: DH
authors.lastname: Hawke
authors.forename: Jeroen
authors.initials: J
authors.lastname: Krijgsveld
authors.forename: William S
authors.initials: WS
authors.lastname: Lane
authors.forename: Kathryn S
authors.initials: KS
authors.lastname: Lilley
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
authors.forename: Robert L
authors.initials: RL
authors.lastname: Moritz
authors.forename: Robert E
authors.initials: RE
authors.lastname: Settlage
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Sherman
authors.forename: Susan T
authors.initials: ST
authors.lastname: Weintraub
authors.forename: H Ewa
authors.initials: HE
authors.lastname: Witkowska
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Yates
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
mesh_terms: D006063:Chorionic Gonadotropin / Q000032:analysis; D019411:Clinical Laboratory Techniques; D024981:Glycogen Phosphorylase / Q000032:analysis; D006801:Humans; D017430:Prostate-Specific Antigen / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000193:education / Q000379:methods*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000032:analysis; D012107:Research Design
chemical_list: D006063:Chorionic Gonadotropin; D011506:Proteins; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D024981:Glycogen Phosphorylase; D017430:Prostate-Specific Antigen