title: The isolation of maxicells.
abstract: The maxicell system (1) is based on the following observation: The number of lesions induced with UV light in a DNA molecule is proportional with the size of the molecule and the dose of the UV light. Since most plasmids are less than 1/1000 the size of the chromosome and are usually present in 10-100 copies per cell, it is possible to irradiate a culture of a plasmid-containing strain in such a way that every single chromosome is damaged at least once, whereas at the same time a substantial number of plasmid molecules remain undamaged.
authors.affiliation: Department of Microbiology, The Technical University of Denmark, Lyngby, Denmark.
authors.forename: W
authors.initials: W
authors.lastname: Gaastra
authors.forename: P
authors.initials: P
authors.lastname: Klemm