title: Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216.
abstract: Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.
authors.affiliation: School of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Li
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Jiang
authors.forename: Huashi
authors.initials: H
authors.lastname: Guan
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
mesh_terms: D000464:Alginates / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D009844:Oligosaccharides; D020723:Glucuronic Acid; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase