title: Antioxidant and signal modulation properties of plant polyphenols in controlling vascular inflammation.
abstract: Oxidized low-density lipoproteins (oxLDL) play a critical role in the initiation of atherosclerosis through activation of inflammatory signaling. In the present work we investigated the role of antioxidant and signal modulation properties of plant polyphenols in controlling vascular inflammation. Significant decrease in intracellular NO level and superoxide overproduction was found in human umbilical vein endothelial cells (HUVEC) treated with oxLDL, but not with LDL. The redox imbalance was prevented by the addition of quercetin or resveratrol. Expression analysis of 14 genes associated with oxidative stress and inflammation revealed oxLDL-mediated up-regulation of genes specifically involved in leukocyte recruitment and adhesion. This up-regulation could be partially avoided by the addition of verbascoside or resveratrol, while treatment with quercetin resulted in a further increase in the expression of these genes. Lipopolysaccharide (LPS)-treated HUVEC were also used for the evaluation of anti-inflammatory potency of plant polyphenols. Significant differences between HUVEC treaded with oxLDL and LPS were found in both the expression pattern of inflammation-related genes and the effects of plant polyphenols on cellular responses. The present data indicate that plant polyphenols may affect vascular inflammation not only as antioxidants but also as modulators of inflammatory redox signaling pathways.
authors.affiliation: Lab. Tissue Engineering And Skin Pathophysiology, Dermatology Institute (IDI IRCCS), Via Monti Di Creta 104, Rome 00167, Italy. kosty-vladimir@yandex.ru
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Kostyuk
authors.forename: Alla I
authors.initials: AI
authors.lastname: Potapovich
authors.forename: Tatyana O
authors.initials: TO
authors.lastname: Suhan
authors.forename: Chiara
authors.initials: C
authors.lastname: de Luca
authors.forename: Liudmila G
authors.initials: LG
authors.lastname: Korkina
mesh_terms: D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001808:Blood Vessels / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000235:genetics; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology* / Q000627:therapeutic use; D010944:Plants / Q000737:chemistry*; D059808:Polyphenols; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D009573:Nitrites; D010636:Phenols; D059808:Polyphenols; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein; D009569:Nitric Oxide; D009195:Peroxidase