title: Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study.
abstract: A simple, sensitive and specific high-performance liquid chromatography mass spectrometry (LC-MS) method was developed and validated for the quantification of strictosamide in dog plasma. Strictosamide and internal standard (IS, ranolazine) extracted by liquid-liquid extraction with ethyl acetate were separated on a C(18) column using a gradient elution program. The detection was performed by selected ion monitoring mode via a positive electrospray ionization interface. The LLOQ was 1.0 ng/mL and the method exhibited acceptable precision, extraction efficiency and matrix effect. Finally, this proposed method was successfully applied to dog pharmacokinetic study and yielded the most comprehensive data on systemic exposure of strictosamide to date.
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, No. 24, Tongjiaxiang Street, Nanjing, 210009, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Dai
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liang
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Ding
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Meng
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Tianye
authors.initials: T
authors.lastname: Guan
authors.forename: An
authors.initials: A
authors.lastname: Kang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.forename: Tong
authors.initials: T
authors.lastname: Xie
authors.forename: Chunzhu
authors.initials: C
authors.lastname: Li
authors.forename: Qijin
authors.initials: Q
authors.lastname: Zhao
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
mesh_terms: D000083:Acetanilides; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D016014:Linear Models; D010879:Piperazines; D000069458:Ranolazine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014748:Vinca Alkaloids / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000083:Acetanilides; D010879:Piperazines; D014748:Vinca Alkaloids; C539462:strictosamide; D000069458:Ranolazine