title: The ultimate Wright-Giemsa stain: 60 years in the making.
abstract: Abstract Wright-Giemsa staining is a common procedure that is performed routinely in hematology laboratories. Consistency in intra-laboratory staining quality is essential for accurate morphological interpretation of blood smears. Although the Wright-Giemsa stain can be challenging to perform, the methods illustrated here have provided consistent, high quality stains in the Special Hematology Laboratory at the University of Minnesota for over half a century. We outline methods for collecting blood specimens, preparing the slides and performing a Wright-Giemsa stain using our combination of reagents. Various techniques that have been passed down in our laboratory for troubleshooting suboptimally stained specimens are shared as well.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, 55455, USA. dunn0161@umn.edu
authors.forename: K
authors.initials: K
authors.lastname: Dunning
authors.forename: A O
authors.initials: AO
authors.lastname: Safo
mesh_terms: D001399:Azure Stains / Q000266:history; D001773:Blood Cells / Q000166:cytology*; D006405:Hematology / Q000266:history / Q000379:methods; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D008853:Microscopy / Q000379:methods; D013194:Staining and Labeling / Q000266:history / Q000379:methods*
chemical_list: D001399:Azure Stains