title: Type I collagen down-regulates E-cadherin expression by increasing PI3KCA in cancer cells.
abstract: The extracellular matrix (ECM) has been shown to induce the epithelial-mesenchymal transition (EMT), which is characterized by the loss of E-cadherin. However, little is known about the mechanisms by which collagen I, the most abundant component of ECM, induces this process. Here, we show that E-cadherin was down-regulated in response to collagen I in SKOV3 ovarian cancer cells and PC3 prostate cancer cells. Furthermore, collagen I induced the up-regulation of PIK3CA, which in turn contributed to the collagen I-induced down-regulation of E-cadherin by up-regulating its transcriptional repressors, Snail and Slug. Our data demonstrate that PIK3CA is a mediator of collagen I-induced down-regulation of E-cadherin.
authors.affiliation: Department of Obstetrics and Gynecology, Child & Family Research Institute, University of British Columbia, Vancouver, Canada.
authors.forename: Jung-Chien
authors.initials: JC
authors.lastname: Cheng
authors.forename: Peter C K
authors.initials: PC
authors.lastname: Leung
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000378:metabolism*; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
chemical_list: D015820:Cadherins; D024042:Collagen Type I; D009687:Nuclear Proteins; C503859:PI3KCA protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors