title: The rational design of nitric oxide selectivity in single-walled carbon nanotube near-infrared fluorescence sensors for biological detection.
abstract: A major challenge in the synthesis of nanotube or nanowire sensors is to impart selective analyte binding through means other than covalent linkages, which compromise electronic and optical properties. We synthesized a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbon nanotubes (SWNTs) that imparts rapid and selective fluorescence detection of nitric oxide (NO), a messenger for biological signalling. The near-infrared (nIR) fluorescence of SWNT(DAP-dex) is immediately and directly bleached by NO, but not by other reactive nitrogen and oxygen species. This bleaching is reversible and shown to be caused by electron transfer from the top of the valence band of the SWNT to the lowest unoccupied molecular orbital of NO. The resulting optical sensor is capable of real-time and spatially resolved detection of NO produced by stimulating NO synthase in macrophage cells. We also demonstrate the potential of the optical sensor for in vivo detection of NO in a mouse model.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Heller
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Paul W
authors.initials: PW
authors.lastname: Barone
authors.forename: Changsik
authors.initials: C
authors.lastname: Song
authors.forename: Jingqing
authors.initials: J
authors.lastname: Zhang
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Trudel
authors.forename: Gerald N
authors.initials: GN
authors.lastname: Wogan
authors.forename: Steven R
authors.initials: SR
authors.lastname: Tannenbaum
authors.forename: Michael S
authors.initials: MS
authors.lastname: Strano
mesh_terms: D000818:Animals; D002460:Cell Line; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008264:Macrophages / Q000737:chemistry; D051379:Mice; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D013237:Stereoisomerism
chemical_list: D005456:Fluorescent Dyes; D037742:Nanotubes, Carbon; D009569:Nitric Oxide