title: [The mechanism and application of zinc finger nucleases].
abstract: Due to specific recognization of DNA sequences and designability, zinc finger nucleases (ZFN) has been used in knock in and knock out genes. Because of high ratio of homologous recombination of DSB-GT induces by ZFN, ZFN technology has been considered as the most powerful tool for gene modification. It has been successfully used at cell or embryo levels in plants and animals. Under the rapid development of high affinity zinc finger protein (ZFP), this technique will be applied to genetic engineering and breeding extensively in the future. This review discussed the DNA recognization mechanism and double strand break gene targeting (DSB-GT) of zinc finger nucleases (ZFN). Some application examples of ZFN were summarized.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. zqiang320@webmail.hzau.edu.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhong
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004720:Endonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting / Q000295:instrumentation; D005818:Genetic Engineering / Q000295:instrumentation; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D016335:Zinc Fingers
chemical_list: D004720:Endonucleases