title: 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene as a bright fluorescent label for DNA.
abstract: 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) as a fluorescent label can be incorporated into DNA by two conceptually different ways: the non-nucleosidic DNA base surrogate Bo exhibits high brightness but no preferential base-pairing properties, whereas the BODIPY-modified uridine BodU has reduced quantum yields but shows preferred Watson-Crick base pairing with adenine.
authors.affiliation: Institute for Organic Chemistry, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ehrenschwender
authors.forename: Hans-Achim
authors.initials: HA
authors.lastname: Wagenknecht
mesh_terms: D020029:Base Pairing; D001896:Boron Compounds; D004396:Coloring Agents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D004247:DNA