title: Expression and purification of recombinant human apolipoprotein C-I in Pichia pastoris.
abstract: Apolipoprotein C-I (ApoC-I) is a small, basic apolipoprotein which is mainly secreted by the liver as a component of triglyceride-rich lipoproteins and high density lipoproteins whose importance in plasma lipoprotein metabolism is increasingly evident. At present, the only way to obtain native ApoC-I is separating it from human plasma. The methods have some restrictions on source, the complicated technology, the potential infections and a high cost which limits the research and application of native ApoC-I. Because of its small size, ApoC-I has previously been prepared by peptide synthesis which is also limited by a high cost. Therefore, in this study, a Pichia pastoris expression system was first used to obtain a high level expression of secreted, recombinant human ApoC-I (rhApoC-I).
authors.affiliation: Department of Biological Engineering, College of Pharmacy, Jilin University, 1266 Fu Jin Road, Changchun 130021, China.
authors.forename: Manman
authors.initials: M
authors.lastname: Su
authors.forename: Yulai
authors.initials: Y
authors.lastname: Zhou
authors.forename: Dingding
authors.initials: D
authors.lastname: Wang
authors.forename: Tianmin
authors.initials: T
authors.lastname: Xu
authors.forename: Weiqin
authors.initials: W
authors.lastname: Chang
authors.forename: Mingxing
authors.initials: M
authors.lastname: Wang
authors.forename: Xuejia
authors.initials: X
authors.lastname: Yu
authors.forename: Dan
authors.initials: D
authors.lastname: Feng
authors.forename: Zhu
authors.initials: Z
authors.lastname: Han
authors.forename: Weiqun
authors.initials: W
authors.lastname: Yan
mesh_terms: D000818:Animals; D053302:Apolipoprotein C-I / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D032389:Myocytes, Smooth Muscle; D010843:Pichia / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C505803:APOC1 protein, human; D053302:Apolipoprotein C-I; D011994:Recombinant Proteins