title: Coagulation and fibrinolysis in amniotic fluid: physiology and observations on amniotic fluid embolism, preterm fetal membrane rupture, and pre-eclampsia.
abstract: Two dangerous obstetric complications, amniotic fluid embolism and preterm prelabor rupture of membranes (PROM), can be caused by amniotic fluid (AF) constituents. Disseminated intravascular coagulation (DIC) is related to the former complication, whereas local thrombin/plasmin-dependent collagenolysis in the decidua and fetal membranes is associated with the latter. In AF, most proteins of the coagulation and fibrinolysis system, known as plasma constituents, have been identified based on the activity and/or presence of antigen. The AF levels of most of these proteins are low (< 2 to 5% of the respective maternal plasma levels). However, there are some exceptions: tissue factor (TF), urokinase plasminogen activator (uPA) and its receptor (uPAR), as well as plasminogen activator inhibitors. The AF level of fetal fibrinogen is trace, which is a particular exception. The key enzymes of coagulation and fibrinolysis, thrombin and plasmin, are generated in AF. Thrombin generation is four- to fivefold higher than in maternal plasma as measured by the concentration of the prothrombin fragments 1 + 2 (F 1 + 2) and thrombin-antithrombin complexes, whereas plasmin generation is relatively low as measured by the plasmin-α-2-antiplasmin complexes. Phosphatidylserine, a phospholipid, and thrombin-activatable fibrinolysis inhibitor (TAFI) are novel components of AF. Phosphatidylserine contributes to DIC in AF embolism; TAFI is considered a link between coagulation and fibrinolysis. uPA and uPAR are the factors contributing to PROM via plasmin-dependent proteolysis. Intriguing is the assumption that TF and its inhibitor can be risk factors for PROM through thrombin-dependent activation of matrix prometalloproteinases in the decidua and fetal membranes. It is unknown whether the amniotic pool of hemostatic components is involved in pre-eclampsia pathogenesis.
authors.affiliation: Department of Propedeutics of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Poland. kizproped@amb.bydgoszcz.pl
authors.forename: Mieczysław
authors.initials: M
authors.lastname: Uszyński
authors.forename: Waldemar
authors.initials: W
authors.lastname: Uszyński
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry / Q000502:physiology*; D001777:Blood Coagulation; D004619:Embolism, Amniotic Fluid / Q000209:etiology*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000209:etiology*; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis / Q000502:physiology; D006801:Humans; D010718:Phosphatidylserines / Q000502:physiology; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D013917:Thrombin / Q000378:metabolism; D013925:Thromboplastin / Q000502:physiology
chemical_list: D010718:Phosphatidylserines; D013925:Thromboplastin; D013917:Thrombin; D005341:Fibrinolysin