title: Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates.
abstract: Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilmforming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent rRNA probes against S. aureus. The results suggest that S. aureus invasion levels are not related to biofilm formation.
authors.affiliation: CIISA, Faculty of Veterinary Medicine, Technical University of Lisbon, 1300-477 Lisbon, Portugal. moliveira@fmv.utl.pt
authors.forename: Manuela
authors.initials: M
authors.lastname: Oliveira
authors.forename: Ricardo
authors.initials: R
authors.lastname: Bexiga
authors.forename: Sandro Filipe
authors.initials: SF
authors.lastname: Nunes
authors.forename: Cristina Lobo
authors.initials: CL
authors.lastname: Vilela
mesh_terms: D000818:Animals; D018441:Biofilms; D002417:Cattle; D002460:Cell Line; D015169:Colony Count, Microbial / Q000662:veterinary; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008414:Mastitis, Bovine / Q000382:microbiology*; D011174:Portugal; D013203:Staphylococcal Infections / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D037521:Virulence Factors / Q000302:isolation & purification
chemical_list: D037521:Virulence Factors