title: Disposable screen-printed electrode coupled with recombinant Drosophila melanogaster acetylcholinesterase and multiwalled carbon nanotubes for rapid detection of pesticides.
abstract: Recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE), multiwalled carbon nanotubes (MWCNTs), and Prussian blue have been combined for development of a three-electrode biosensor with more rapid responses and higher stability than in our previous study. A new disposable screen-printed electrode (SPE) was developed for rapid detection of organophosphate and carbamate pesticides. After optimization, 10 microg MWCNT and 5 microL enzyme immobilization solution consisting of 0.2% glutaraldehyde, 0.1% Nafion, 0.2% bovine serum albumin, 0.1 g/L MWCNT, and 1.5 mU R-DmAChE were fixed on each of the R-DmAChE/MWCNT SPEs. The LOD of this biosensor was 0.5 microg/L for pesticide standards of dichlorvos (DDV) and carbofuran. The performance of this biosensor was tested for vegetable and water samples at various spiked levels, and good stability and sensitivity were found. The obtained recoveries were from 82.6 to 110.5% for DDV at levels of 0.5-5 microg/L and 73.4 to 118.4% for carbofuran at 1-10 microg/L in lake and sea water samples, demonstrating that the proposed approach is an alternative means for rapid detection of pesticide residues and contaminants in food safety and environmental monitoring.
authors.affiliation: Shanghai Jiao Tong University, School of Life Science and Biotechnology, Bor Luh Food Safety Center, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China.
authors.forename: Zhuzhao
authors.initials: Z
authors.lastname: Tang
authors.forename: Haode
authors.initials: H
authors.lastname: Chen
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.forename: Dabing
authors.initials: D
authors.lastname: Zhang
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
mesh_terms: D000110:Acetylcholinesterase; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002235:Carbofuran / Q000032:analysis; D002417:Cattle; D004006:Dichlorvos / Q000032:analysis; D004209:Disposable Equipment; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology; D004566:Electrodes; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D005506:Food Contamination / Q000032:analysis; D037742:Nanotubes, Carbon; D010575:Pesticides / Q000032:analysis*; D011327:Printing; D011994:Recombinant Proteins; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D029721:Drosophila Proteins; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D037742:Nanotubes, Carbon; D010575:Pesticides; D011994:Recombinant Proteins; D014874:Water Pollutants, Chemical; D004006:Dichlorvos; D000110:Acetylcholinesterase; D002235:Carbofuran; C000170:ferric ferrocyanide