title: [Experimental and computational approach of calcium signaling].
abstract: In many cell types, specific and robust signalling relies on a high level of spatiotemporal organization of Ca(2+) dynamics. In response to external stimulation, Ca(2+) signals ranging from a small increase of a few tens of nanomolar concentrations at the mouth of an inositol 1, 4, 5-trisphosphate receptor to the periodic propagation of waves invading an organ or a tissue, can be observed. Here, we review our combined experimental and computational approach of Ca(2+) dynamics, which has been mainly carried out on liver hepatocytes. We focus in particular on the understanding of the relationship between elementary Ca(2+) increases, Ca(2+) oscillations and intra- or intercellular Ca(2+) waves. The physiological impact of such signalling on liver function is also discussed.
authors.affiliation: UMR-S757, Inserm, Université Paris-Sud, Orsay, F-91405, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Combettes
authors.forename: Geneviève
authors.initials: G
authors.lastname: Dupont
mesh_terms: D020013:Calcium Signaling; D002477:Cells / Q000378:metabolism*; D008099:Liver / Q000502:physiology; D008954:Models, Biological