title: Marine isolate Citricoccus sp. KMM 3890 as a source of a cyclic siderophore nocardamine with antitumor activity.
abstract: A novel actinobacterium, designated KMM 3890 was isolated from a bottom sediment sample collected from the Sakhalin shallow environment. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated strain KMM 3890 affiliation to the genus Citricoccus. In addition to its hemolytic activity, this strain exhibited inhibitory activity against Gram-positive bacteria. It was found that the marine isolate Citricoccus sp. KMM 3890 produced and excreted into the culture medium a large amount of the compound, which was isolated and structurally characterized as known cyclic siderophore nocardamine on the basis of combined spectral analyses. Nocardamine showed inhibitory effects to colony formation of T-47D, SK-Mel-5, SK-Mel-28 and PRMI-7951 tumor cell lines and a weak antimicrobial against Gram-positive bacteria and no revealed cytotoxic activity. This study can be considered as the first report on marine isolate of the genus Citricoccus producing nocardamine with antitumor activity.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, Vladivostok, Russian Federation. schuka@piboc.dvo.ru
authors.forename: Nataliya I
authors.initials: NI
authors.lastname: Kalinovskaya
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Romanenko
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Irisawa
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Ermakova
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Kalinovsky
mesh_terms: D000898:Antibiosis; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D008835:Micrococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013057:Spectrum Analysis; D013234:Stem Cells
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; C033516:deferrioxamine E