title: An end-to-end anastomosis model of guinea pig bile duct: A 6-mo observation.
abstract: OBJECTIVE
  To establish the end-to-end anastomosis (EEA) model of guinea pig bile duct and evaluate the healing process of bile duct.
  
  
  METHODS
  Thirty-two male guinea pigs were randomly divided into control group, 2-, 3-, and 6-mo groups after establishment of EEA model. Histological, immunohistochemical and serologic tests as well as measurement of bile contents were performed. The bile duct diameter and the diameter ratio (DR) were measured to assess the formation of relative stricture.
  
  
  RESULTS
  Acute and chronic inflammatory reactions occurred throughout the healing process of bile duct. Serology test and bile content measurement showed no formation of persistent stricture in 6-mo group. The DR revealed a transient formation of relative stricture in 2-mo group in comparation to control group (2.94 ± 0.17 vs 1.89 ± 0.27, P = 0.004). However, this relative stricture was released in 6-mo group (2.14 ± 0.18, P = 0.440).
  
  
  CONCLUSIONS
  A simple and reliable EEA model of guinea pig bile duct can be established with a good reproducibility and a satisfactory survival rate.
authors.affiliation: Department of General Surgery, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Yuan-Hu
authors.initials: YH
authors.lastname: Tian
authors.forename: Zhi
authors.initials: Z
authors.lastname: Xu
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Wang
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Hou
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Ling
authors.forename: Xiao-Si
authors.initials: XS
authors.lastname: Zhou
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001652:Bile Ducts / Q000166:cytology / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D003251:Constriction, Pathologic; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D023421:Models, Animal; D011183:Postoperative Complications; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011897:Random Allocation; D014945:Wound Healing
chemical_list: D018809:Proliferating Cell Nuclear Antigen