title: Correlation between in vitro mucus adhesion and the in vivo colonization ability of lactic acid bacteria: screening of new candidate carp probiotics.
abstract: We measured the adhesion of candidate probiotic lactic acid bacteria (LAB) to carp intestinal mucus. The percentage of adherent bacteria varied among strains. Four strains, two with high adhesion and two with low adhesion in vitro, were tested for in vivo colonization ability. Carp were fed LAB-containing feed for 12 d, and then unsupplemented feed until day 33, and the numbers and compositions of intestinal LAB were analyzed during the entire period. LAB with lower in vitro adhesion disappeared quickly from the intestine after LAB feeding stopped. LAB with higher in vitro adhesion remained in the intestine 3 weeks after LAB feeding stopped, indicating a strong correlation between mucus adhesion in vitro and colonization ability in vivo. Next we isolated nine candidate probiotic LAB with high in vitro mucus-binding ability. Three of them were fed to carp, and all three were stably maintained in the intestine.
authors.affiliation: Division of Integrative Environmental Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sugimura
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Hagi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hoshino
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D015373:Bacterial Typing Techniques; D002347:Carps / Q000382:microbiology; D015169:Colony Count, Microbial; D007413:Intestinal Mucosa / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D019344:Lactic Acid / Q000378:metabolism; D007777:Lactobacillaceae / Q000378:metabolism*; D009093:Mucus / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019936:Probiotics / Q000032:analysis / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: D019344:Lactic Acid