title: Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain.
abstract: Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.
authors.affiliation: Department of Microbiology, Dartmouth Medical School, Hanover, NH 03755, USA.
authors.forename: Dhanalakshmi
authors.initials: D
authors.lastname: Nair
authors.forename: Guido
authors.initials: G
authors.lastname: Memmi
authors.forename: David
authors.initials: D
authors.lastname: Hernandez
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bard
authors.forename: Marie
authors.initials: M
authors.lastname: Beaume
authors.forename: Steven
authors.initials: S
authors.lastname: Gill
authors.forename: Patrice
authors.initials: P
authors.lastname: Francois
authors.forename: Ambrose L
authors.initials: AL
authors.lastname: Cheung
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D056084:Genetic Fitness; D016680:Genome, Bacterial; D009154:Mutation; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D013312:Stress, Physiological; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D037521:Virulence Factors