title: Label-free detection with surface plasmon resonance imaging.
abstract: Sensors based on surface plasmon resonance have demonstrated, over the last 2 decades, to be an effective method of studying biomolecular interactions without the need for labeling. Recently, it has been adapted to high-throughput use for imaging microarray binding in real time. This provides a promising platform - a label-free protein microarray system - for the study of disease. In this example, antibody microarrays are used to efficiently profile the secretion of proteins from a cell line exposed to varying concentrations of a toxic compound.
authors.affiliation: Institute for Systems Biology, Seattle, WA, USA. clausted@systemsbiology.org
authors.forename: Christopher
authors.initials: C
authors.lastname: Lausted
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
mesh_terms: D000906:Antibodies / Q000276:immunology; D002839:Chromans / Q000494:pharmacology; D003470:Culture Media / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D011327:Printing; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D045162:Thiazolidinediones / Q000494:pharmacology; D000077288:Troglitazone
chemical_list: D000906:Antibodies; D002839:Chromans; D003470:Culture Media; D011506:Proteins; D045162:Thiazolidinediones; D000077288:Troglitazone