title: Structure elucidation of gluten-derived peptides by tandem mass spectrometry.
abstract: In recent years there have been major developments in the field of mass spectrometry (MS) that permit the analysis and characterization of peptides and proteins at the femtomolar level (1-8). This chapter deals with the use of MS for the elucidation of peptide sequences of gliadin- and/or glutenin-derived fragments. It is meant to give an overview of what modern mass spectrometric methods can mean for your research in the biomolecular field rather than an in-depth step-by-step practical protocol. Important aspects are covered to give the reader an idea of what is needed to perform, and what can be expected from, this mass spectrometric application.
authors.affiliation: Department of Immunohaematology and Bloodbank, University of Leiden, Leiden, The Netherlands.
authors.forename: P A
authors.initials: PA
authors.lastname: van Veelen
authors.forename: J W
authors.initials: JW
authors.lastname: Drijfhout
authors.forename: F
authors.initials: F
authors.lastname: Koning