title: Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil.
abstract: BACKGROUND
  This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil.
  
  
  METHODS
  Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays.
  
  
  RESULTS
  It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1.
  
  
  CONCLUSIONS
  The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.
authors.affiliation: Departamento de Clínica Médica Veterinária, Universidade Federal de Mato Grosso, Brazil. z82prmar@uco.es
authors.forename: Rodrigo Prado
authors.initials: RP
authors.lastname: Martins
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: da Silva
authors.forename: Valeria
authors.initials: V
authors.lastname: Dutra
authors.forename: Luciano
authors.initials: L
authors.lastname: Nakazato
authors.forename: Domingos da Silva
authors.initials: Dda S
authors.lastname: Leite
mesh_terms: D000003:Abattoirs; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004768:Enterotoxins / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics; D007422:Intestines / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C027538:heat-labile enterotoxin, E coli