title: Transcriptional regulation of proteoglycans and glycosaminoglycan chain-synthesizing glycosyltransferases by UV irradiation in cultured human dermal fibroblasts.
abstract: Various kinds of glycosaminoglycans (GAGs) and proteoglycans (PGs) have been known to be involved in structural and space-filling functions, as well as many physiological regulations in skin. To investigate ultraviolet (UV) radiation-mediated regulation of GAGs and PGs in cultured human dermal fibroblasts, transcriptional changes of many types of PGs and GAG chain-synthesizing enzymes at 18 hr after 75 mJ/cm(2) of UV irradiation were examined using quantitative real-time polymerase chain reaction methods. Hyaluronic acid synthase (HAS)-1, -2, and -3 and hyaluronidase-2 mRNA expressions were significantly increased by UV irradiation. Expressions of lumican, fibromodulin, osteoglycin, syndecan-2, perlecan, agrin, versican, decorin, and biglycan were significantly decreased by UV irradiation, while syndecan-1 was increased. Expressions of GAG chain-synthesizing glycosyltransferases, xylosyltransferase-1, β1,3-glucuronyltransferase-1, β1,4-galactosyltransferase-2, -4, exostosin-1, chondroitin polymerizing factor, and chondroitin sulfate synthase-3 were significantly reduced, whereas those of β1,3-galactosyltransferase-6, β1,4-galactosyltransferase-3, -7, β-1,3-N-acetylglucosaminyltran sferase-2, and -7 were increased by UV irradiation. Heparanase-1 mRNA expression was increased, but that of heparanase-2 was reduced by UV irradiation. Time-course investigation of representative genes showed consistent results. In conclusion, UV irradiation may increase hyaluronic acid production through HAS induction, and decrease other GAG productions through downregulation of PG core proteins and GAG chain-synthesizing glycosyltransferases in cultured human dermal fibroblasts.
authors.affiliation: Department of Dermatology, Institute of Dermatological Science, Seoul National University College of Medicine, University Hospital, 101 Daehang-no, Seoul, Korea.
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Shin
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Oh
authors.forename: Yeon Kyung
authors.initials: YK
authors.lastname: Kim
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Jung
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects; D014453:Glucuronosyltransferase / Q000235:genetics / Q000528:radiation effects; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000737:chemistry; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000096:biosynthesis; D006821:Hyaluronoglucosaminidase / Q000235:genetics / Q000528:radiation effects; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics / Q000528:radiation effects; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D012333:RNA, Messenger; D006820:Hyaluronic Acid; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases; D006821:Hyaluronoglucosaminidase