title: Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test.
abstract: This experiment was conducted to study the genotoxic potentials of sodium arsenite (NaAsO(2)) in freshwater fish Oreochromis mossambicus by using alkaline comet assay and micronucleus (MN) test. Fish were exposed to three different concentrations (3 ppm, 28 ppm and 56 ppm) of arsenic and gill, liver and blood tissue samples were collected after 48 h, 96 h and 192 h of exposure. Arsenic exposure induced DNA damage in all tissues examined in a concentration dependent manner. A significant (p<0.05) increase in the comet tail DNA (%) of the exposed fish liver, gill, and blood was observed after 48 h and 96 h of exposure, but a decline in DNA damage was recorded in all the tissues at all the three concentrations studied after 192 h of exposure. Liver tissue exhibited significantly (p<0.05) higher DNA damage at all the concentrations examined, followed by gill and blood. Higher liver tail DNA (51.38 ± 0.21%) refers that it is more prone to injury to arsenic toxicity than the gill and blood. In blood samples arsenic induced micronucleus formation in a concentration dependent manner and highest (5.8 ± 0.46%) value was recorded in 56 ppm after 96 h of exposure, whereas, it was decreased after 192 h of exposure at all the three concentrations of NaAsO(2) examined which refers to the DNA repairing ability of fish to arsenic toxicity. The results of this study depict the genotoxic potentials of arsenic to fish which in turns provide insight on advanced study in aquatic toxicology.
authors.affiliation: Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh. kawser@univdhaka.edu
authors.forename: Md Kawser
authors.initials: MK
authors.lastname: Ahmed
authors.forename: Md
authors.initials: M
authors.lastname: Habibullah-Al-Mamun
authors.forename: M Anwar
authors.initials: MA
authors.lastname: Hossain
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arif
authors.forename: Elora
authors.initials: E
authors.lastname: Parvin
authors.forename: Mosammat Salma
authors.initials: MS
authors.lastname: Akter
authors.forename: Mohammad Shahneawz
authors.initials: MS
authors.lastname: Khan
authors.forename: Md Monirul
authors.initials: MM
authors.lastname: Islam
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005618:Fresh Water / Q000737:chemistry; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D017210:Tilapia / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical; D001151:Arsenic