title: MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration.
abstract: BACKGROUND
  To determine the effect of higher progesterone (P) level on endometrial receptivity.
  
  
  METHODS
  This was a prospective analysis conducted in the Reproductive Medical Center of Peking University Third Hospital. All patients received IVF treatment and canceled embryo transfer in the same cycle and were divided into group 1 (normal P; 7 patients) and group 2 (elevated P; 12 patients). Endometrial biopsies were performed 6 days after oocyte retrieval. The global miRNA and mRNA gene expressions in endometrial biopsies were investigated with a V4.0 miRNA probe and 22 K Human Genome Array. Fold ratios were derived to compare gene regulation between the groups. Spp1 and Ang gene expression was selected to verify the array results by RT-PCR and the protein expression of osteopontin and VEGF was determined using an immunohistochemical method.
  
  
  RESULTS
  There were 4 miRNA (all down-regulated) and 22 mRNA (13 up-regulated and 9 down-regulated) exhibiting differential expression between the groups on the microRNA and microarray chips. miRNA-451, Spp1, and Ang expression in RT-PCR verified the array results. Osteopontin and VEGF were also shown to have positive expression in the endometrium.
  
  
  CONCLUSIONS
  Data from microRNA and microarray analysis suggests dissimilar endometrial receptivity in patients with high P levels on the day of hCG, and elevated osteopontin and decreased VEGF had poor pregnancy rates.
authors.affiliation: Peking University Third Hospital, No, 49, North Huayuan Road, Haidian District, Beijing 100191, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xiumei
authors.initials: X
authors.lastname: Zhen
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000328:Adult; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D015536:Down-Regulation; D004717:Endometrium / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D053495:Osteopontin / Q000096:biosynthesis; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood*; D040081:Protein Array Analysis; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: D006063:Chorionic Gonadotropin; D035683:MicroRNAs; C506895:SPP1 protein, human; D042461:Vascular Endothelial Growth Factor A; D053495:Osteopontin; D011374:Progesterone