title: Molecularly imprinted matrix solid-phase dispersion combined with dispersive liquid-liquid microextraction for the determination of four Sudan dyes in egg yolk.
abstract: A new kind of aniline-naphthol molecularly imprinted microsphere (MIM) synthesized by aqueous suspension polymerization was applied as a selective sorbent of miniaturized matrix solid-phase dispersion combining with dispersive liquid-liquid microextraction (MSPD-DLLME) for the simultaneous determination of four Sudans in egg yolk samples. The solid sample was directly blended with MIM in MSPD procedure and the eluent of MSPD was used as the dispersive solvent of the followed DLLME for further purification and enrichment of the analytes before HPLC analysis. Good linearity for all the Sudan dyes was ranged from 0.02 μg g(-1) to 2.0 μg g(-1) (r(2)≥0.9990) and their recoveries at three spiked levels were ranged from 87.2% to 103.5% with RSD less than 6.1% (n=3). The presented MIM-MSPD-DLLME method combined the advantages of MIM, MSPD and DLLME, and could be applied for the determination of Sudans in complicated food samples.
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China. yanhy@hbu.edu.cn
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jindong
authors.initials: J
authors.lastname: Qiao
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D001391:Azo Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000032:analysis*; D004530:Egg Yolk / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D016014:Linear Models; D008863:Microspheres; D054802:Molecular Imprinting; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000814:Aniline Compounds; D001391:Azo Compounds; D004396:Coloring Agents; C023650:aniline