title: Anthocyanin characterization utilizing liquid chromatography combined with advanced mass spectrometric detection.
abstract: Anthocyanins are naturally occurring polyphenolic plant pigments. To analyze the anthocyanin content of samples, rapid and reliable methods for separation and detection are needed. In this work an LC-DAD-ESI-MS/MS instrument was used to develop a new tandem MS data acquisition strategy for anthocyanin characterization which was subsequently compared to more conventional measurements. It has been shown that the newly developed strategy, multiple reaction monitoring-initiated anthocyanin characterization (MIAC), can successfully be used in anthocyanin analysis and has various advantages compared to some more traditional measurements, such as enhanced selectivity, better signal-to-noise ratio and simplified data evaluation. Furthermore, the number of relevant MS/MS data increased significantly with the MIAC method compared with a more common information dependent MS experiment strategy.
authors.affiliation: Department of Physical and Analytical Chemistry, Uppsala University, Box 599, SE-751 24 Uppsala, Sweden.
authors.forename: Sarah
authors.initials: S
authors.lastname: Steimer
authors.forename: Per J R
authors.initials: PJ
authors.lastname: Sjöberg
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D031985:Fragaria / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D019697:Onions / Q000737:chemistry; D027861:Prunus / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000872:Anthocyanins