title: [Preparative isolation and purification of the active components from Viticis Fructus by high-speed counter-current chromatography].
abstract: Vitex trifolia L. var. simplicifolia Cham. is widely distributed in Asia, and its fruits are used as a folk medicine for headaches, colds, migraine, eyepain, etc. In order to effectively separate high-purity active components from the seeds of Vitex trifolia L. var. simplicifolia Cham., a high-speed counter-current chromatography (HSCCC) procedure was performed to separate four components from the crude extract of the fruits. A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water (3:6: 3.6: 3, v/v/v/ v) was used. Within 230 min, 23 mg of 4-hydroxybenzoic acid, 15 mg of 3,6,7-trimethylquercetagetin, 24 mg of casticin and 5 mg of artemetin were obtained from 250 mg of the crude extract of Viticis Fructus in one-step elution under the conditions of a flow rate of 1.5 mL/min, 800 r/min and the detection wavelength of 254 nm. The purities of the four fractions were 93.1%, 97.3%, 98.7% and 98.5%, respectively. The obtained fractions were analyzed by high performance liquid chromatography (HPLC), and identified by electrospray ionization mass spectrometry (ESI-MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR. The results indicate that HSCCC is a powerful technique for the purification of active components from Viticis Fructus.
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Guan
authors.forename: Daijie
authors.initials: D
authors.lastname: Wang
authors.forename: Zongyuan
authors.initials: Z
authors.lastname: Yu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Lan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D010226:Parabens / Q000032:analysis*; D012639:Seeds / Q000737:chemistry; D020950:Vitex / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010226:Parabens; C054132:artemetin; C054133:casticin; C038193:4-hydroxybenzoic acid