title: Aldh2 knockout mice were more sensitive to DNA damage in leukocytes due to ethyl tertiary butyl ether exposure.
abstract: To clarify the genotoxicity of ethyl tertiary butyl ether (ETBE), a gasoline additive, male and female C57BL/6 mice of Aldh2+/+ and Aldh2-/- genotypes, aged 8 wk, were exposed to 0, 500, 1,750, or 5,000 ppm ETBE for 6 h/day, 5 d per week for 13 wk. DNA damage in leukocytes was measured by the alkaline comet assay and expressed quantitatively as Tail Intensity (TI). For male mice, TI was significantly higher in all three groups exposed to ETBE than in those without exposure within Aldh2-/- mice, whereas within Aldh2+/+ mice, TI increased only in those exposed to 5,000 ppm of ETBE as compared with mice without exposure. For female mice, a significant increase in TI values was observed in the group exposed to 5,000 ppm of ETBE as compared with those without exposure within Aldh2-/- mice; TI in Aldh2-/- mice exposed to 1,750 and 5,000 ppm was significantly higher than in Aldh2+/+ mice without exposure. TI did not significantly increase in any of the groups exposed to ETBE within female Aldh2+/+ mice. Based on the results we suggest that Aldh2-/- mice are more sensitive to DNA damage caused by ETBE than Aldh2+/+ mice and that males seem more susceptible to this effect than females.
authors.affiliation: National Institute of Occupational Safety and Health, Japan.
authors.forename: Zuquan
authors.initials: Z
authors.lastname: Weng
authors.forename: Megumi
authors.initials: M
authors.lastname: Suda
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ohtani
authors.forename: Nan
authors.initials: N
authors.lastname: Mei
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Tamie
authors.initials: T
authors.lastname: Nakajima
authors.forename: Rui-Sheng
authors.initials: RS
authors.lastname: Wang
mesh_terms: D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000704:Analysis of Variance; D000818:Animals; D004249:DNA Damage / Q000235:genetics*; D004195:Disease Models, Animal; D005019:Ethyl Ethers / Q000633:toxicity*; D005260:Female; D019570:Inhalation Exposure / Q000009:adverse effects*; D007962:Leukocytes; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012737:Sex Factors; D013223:Statistics as Topic
chemical_list: D005019:Ethyl Ethers; C098546:ethyl tert-butyl ether; C515029:ALDH2 protein, mouse; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial