title: Three-dimensional immunohistochemical characterization of lineage commitment by localization of T and FOXA2 in porcine peri-implantation embryos.
abstract: Gene expression during gastrulation in porcine embryos has been sparsely studied, but there are indications that species-specific patterns exist. Here, we investigated the three-dimensional (3D) expression of the T-box transcription factor Brachyury (T) and the forkhead box transcription factor FOXA2 by immunocytochemistry in porcine peri-gastrulation embryos. The first T(+) cells were detected in posterior epiblast of ovoid blastocysts. Later T(+) FOXA2(-) cells were found in the posterior primitive streak (PS) and nascent mesoderm, T(+) FOXA2(+) cells in the anterior PS, probably identifying the organizer region, and T(-) FOXA2(+) cells anterior to this region. In embryos with a neural groove, T and FOXA2 were co-expressed in the node and notochord, FOXA2 was expressed in the floor plate and posteriorly T was expressed in the streak. In all developmental stages, FOXA2 was expressed in the entire hypoblast/definitive endoderm. We conclude that the expression pattern of T and FOXA2 is largely conserved between pig and mouse.
authors.affiliation: Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
authors.forename: Xenia Asbaek
authors.initials: XA
authors.lastname: Wolf
authors.forename: Palle
authors.initials: P
authors.lastname: Serup
authors.forename: Poul
authors.initials: P
authors.lastname: Hyttel
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D017124:Conserved Sequence; D004622:Embryo, Mammalian / Q000378:metabolism*; D005326:Fetal Proteins / Q000378:metabolism*; D051542:Hepatocyte Nuclear Factor 3-beta / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D034421:Sus scrofa / Q000196:embryology*; D020825:T-Box Domain Proteins / Q000378:metabolism*; D000098908:Brachyury Protein
chemical_list: D005326:Fetal Proteins; D051542:Hepatocyte Nuclear Factor 3-beta; D020825:T-Box Domain Proteins; D000098908:Brachyury Protein