title: A candidate approach implicates the secreted Salmonella effector protein SpvB in P-body disassembly.
abstract: P-bodies are dynamic aggregates of RNA and proteins involved in several post-transcriptional regulation processes. P-bodies have been shown to play important roles in regulating viral infection, whereas their interplay with bacterial pathogens, specifically intracellular bacteria that extensively manipulate host cell pathways, remains unknown. Here, we report that Salmonella infection induces P-body disassembly in a cell type-specific manner, and independently of previously characterized pathways such as inhibition of host cell RNA synthesis or microRNA-mediated gene silencing. We show that the Salmonella-induced P-body disassembly depends on the activation of the SPI-2 encoded type 3 secretion system, and that the secreted effector protein SpvB plays a major role in this process. P-body disruption is also induced by the related pathogen, Shigella flexneri, arguing that this might be a new mechanism by which intracellular bacterial pathogens subvert host cell function.
authors.affiliation: RNA Biology Group, Max Planck Institute for Infection Biology, Berlin, Germany. ana.eulalio@icgeb.org
authors.forename: Ana
authors.initials: A
authors.lastname: Eulalio
authors.forename: Kathrin S
authors.initials: KS
authors.lastname: Fröhlich
authors.forename: Miguel
authors.initials: M
authors.lastname: Mano
authors.forename: Mauro
authors.initials: M
authors.lastname: Giacca
authors.forename: Jörg
authors.initials: J
authors.lastname: Vogel
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D022082:Cellular Structures / Q000378:metabolism* / Q000382:microbiology*; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis; D012475:Salmonella / Q000166:cytology / Q000378:metabolism*; D012480:Salmonella Infections / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism; D012760:Shigella / Q000378:metabolism; D037521:Virulence Factors
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; C440638:SPI-2 protein, Salmonella; D037521:Virulence Factors; D036002:ADP Ribose Transferases; C415223:spvB protein, Salmonella enterica virulence plasmid