title: Effect of piperacillin-tazobactam coated β-tricalcium phosphate for mastoid obliteration in otitis media.
abstract: OBJECTIVE
  β-Tricalcium phosphate (TCP) has good biodegradability and osteoconductivity as a scaffold material for bone tissue engineering. Both block and granular forms are available; however, it has been associated with risk of infection and exposure. To this end, the study evaluated the effect of piperacillin-tazobactam coated β-TCPs for mastoid obliteration in otitis media.
  
  
  METHODS
  Ten guinea pigs were divided into the experimental (piperacillin-tazobactam coated β-TCP granules, n=5) and control groups (uncoated β-TCP granules, n=5). After mastoid obliteration, transtympanic injection with a saline suspension of lipopolysaccharide established inflammation. The animals were sacrificed 5 weeks later. Tissue sections were stained with hematoxylin and eosin and examined.
  
  
  RESULTS
  Encapsulation and formation of fibrous capsule by foreign material in the bulla were not evident. The histological evaluation did not reveal inflammatory cells and fibrosis in the piperacillin-tazobactam coated β-TCP group. In contrast, the control group showed numerous inflammatory cells around the implanted uncoated β-TCP granules and incomplete new bone formation.
  
  
  CONCLUSIONS
  β-TCP is an effective carrier material for piperacillin-tazobactam. The use of piperacillin-tazobactam coated β-TCP may be optimal for mastoid obliteration.
authors.affiliation: Department of Otolaryngology, Chonnam National University Medical School, Gwangju 501-757, South Korea; Research Center for Resistant Cells, Chosun University, Gwangju, South Korea.
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Jang
authors.forename: Yong Bum
authors.initials: YB
authors.lastname: Cho
authors.forename: Hyung Chae
authors.initials: HC
authors.lastname: Yang
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Cheol Hee
authors.initials: CH
authors.lastname: Choi
authors.forename: Sook-Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Haekyun
authors.initials: H
authors.lastname: Park
authors.forename: Geun Hyung
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible; D004195:Disease Models, Animal; D006168:Guinea Pigs; D008297:Male; D008416:Mastoid / Q000187:drug effects* / Q000601:surgery; D010033:Otitis Media / Q000175:diagnosis / Q000601:surgery*; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology; D000077725:Piperacillin, Tazobactam Drug Combination; D011897:Random Allocation; D019651:Plastic Surgery Procedures / Q000379:methods; D012016:Reference Values; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014433:Tympanoplasty / Q000379:methods
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; C485817:beta-tricalcium phosphate; D000077725:Piperacillin, Tazobactam Drug Combination; D010397:Penicillanic Acid; D010878:Piperacillin