title: Identification of a novel fosfomycin resistance gene (fosA2) in Enterobacter cloacae from the Salmon River, Canada.
abstract: OBJECTIVE
  To investigate the occurrence of fosfomycin-resistant (fos(R) ) bacteria in aquatic environments.
  
  
  RESULTS
  A fos(R) strain of Enterobacter cloacae was isolated from a water sample collected at a site (50°41'33·44″N, 119°19'49·50″W) near the mouth of the Salmon River at Salmon Arm, in south-central British Columbia, Canada. The strain was identified by PCR screening for plasmid-borne, fosA-family amplicons, followed by selective plating. Sequencing of the resistance gene cloned using PCR primers to conserved flanking DNA revealed a new allele (95% amino acid identity to fosA), and I-Ceu I PFGE showed that it was chromosomally located. In Escherichia coli, the cloned DNA conferred a greater resistance to fosfomycin than its fosA counterpart.
  
  
  CONCLUSIONS
  Gene fosA2 conferred fosfomycin resistance in an environmental isolate of Ent. cloacae.
  
  
  CONCLUSIONS
  The repurposing of older antibiotics should be considered in the light of existing reservoirs of resistance genes in the environment.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China. haixu@sdu.edu.cn
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: V
authors.initials: V
authors.lastname: Miao
authors.forename: W
authors.initials: W
authors.lastname: Kwong
authors.forename: R
authors.initials: R
authors.lastname: Xia
authors.forename: J
authors.initials: J
authors.lastname: Davies
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D001955:British Columbia; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D005578:Fosfomycin / Q000494:pharmacology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D045483:Rivers / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D005578:Fosfomycin