title: In situ hybridization analysis of transgenic tissues.
abstract: In situ hybridization (ISH), also called in situ hybridohistochemistry (ISHH), has been used since the 1970s to localize mRNA within a tissue. This technique is now routine in many laboratories, and some standard procedures are emerging.
authors.affiliation: Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.
authors.forename: J M
authors.initials: JM
authors.lastname: Funkhouser
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D002021:Buffers; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014166:Transferases / Q000378:metabolism
chemical_list: D002021:Buffers; D015345:Oligonucleotide Probes; D014166:Transferases