title: Comparison of immunoreactivities in 4-HNE and superoxide dismutases in the cervical and the lumbar spinal cord between adult and aged dogs.
abstract: Aging shows slowly progressive changes and is associated with many functional and morphological changes in the central nervous system. The accumulation of reactive oxygen species causes age-related deterioration in neuronal function and contributes to the increase of disease susceptibility during normal aging. In the present study, we compared the neuronal distribution and immunoreactivities of 4-hydroxy-2E-nonenal (4-HNE, end product of lipid peroxidation), and superoxide dismutase 1 (SOD1) and SOD2 in the cervical and lumbar spinal cord between adult (2-3 years) and aged (10-12 years) dogs. No significant change in neuronal morphology was observed after cresyl violet staining. The number of NeuN (a marker for neurons)-immunoreactive neurons was not significantly changed in the aged group compare to the adult group. In addition, we could not find Fluoro-Jade B (a marker for degenerating neurons) positive cells in both the adult and aged dogs. However, numbers of 4-HNE-, SOD1- and SOD2-immunoreactive cells were significantly increased in both the cervical and lumbar spinal cord of the aged dog: The increase rates of these cells in the aged spinal cord were higher in the lumbar level than the cervical level. In brief, 4-HNE, SOD1 and SOD2 levels are much increased in the aged spinal cord compared to the adult spinal cord.
authors.affiliation: Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Gyeongsan 712-714, South Korea.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Jin Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Choong Hyun
authors.initials: CH
authors.lastname: Lee
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Yun Lyul
authors.initials: YL
authors.lastname: Lee
authors.forename: Hyung-Cheul
authors.initials: HC
authors.lastname: Shin
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D002574:Cervical Vertebrae; D004285:Dogs; D007150:Immunohistochemistry; D008159:Lumbar Vertebrae; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D013482:Superoxide Dismutase; C027576:4-hydroxy-2-nonenal