title: Cytoplasmic intermediate filaments mediate actin-driven positioning of the nucleus.
abstract: The localization of the nucleus is precisely regulated, and defects in nuclear positioning are observed in diseases such as lissencephaly, cerebellar ataxia and dysplasia. We show here that cytoplasmic intermediate filaments are essential players in actin-dependent positioning of the nucleus. The actin retrograde flow is relayed by a flow of intermediate filaments that accumulate asymmetrically around the nuclear envelope. Perturbations of the intermediate filament network alter positioning of the nucleus in both migrating and immobile astrocytes. This function of intermediate filaments might be crucial for regulating cell motility, in particular in tumor cells expressing high levels of cytoplasmic intermediate filaments.
authors.affiliation: Institut Pasteur, Cell polarity and Migration Group and CNRS URA 2582, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dupin
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Sandrine
authors.initials: S
authors.lastname: Etienne-Manneville
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D007382:Intermediate Filaments / Q000378:metabolism*; D051379:Mice; D009685:Nuclear Envelope / Q000378:metabolism; D051381:Rats
chemical_list: D000199:Actins