title: Limited DNA damage in human endothelial cells after hyperbaric oxygen treatment and protection from subsequent hydrogen peroxide exposure.
abstract: BACKGROUND
  In vitro studies on hyperbaric oxygen (HBO) therapy suggest that HBO may cause DNA damage, but this has not been evaluated using endothelial cells.
  
  
  METHODS
  Human umbilical cord endothelial cells (HUVECs) were exposed either to H(2)O(2) or to HBO for 90 min, with or without subsequent H(2)O(2) exposure. Measurements included the comet assay for DNA damage, and reduced and oxidised glutathione levels.
  
  
  RESULTS
  HUVECs showed sensitivity to H(2)O(2) (EC(50) of 0.2mM for DNA migration). A single 90 min HBO treatment at 2.2 ATA caused a statistically significant (ANOVA, P<0.05) increase of DNA migration in HUVECs to 6.8 ± 0.3% (mean ± SEM, n=8), which returned to normal levels (4.9 ± 0.1%, n=6) after 24h. Further exposure to 0.2mM H(2)O(2) after HBO treatment significantly increased the DNA migration in HBO-treated cells immediately post-treatment; but 24h later the cells showed 22% less DNA damage and higher glutathione than controls.
  
  
  CONCLUSIONS
  A single HBO exposure causes limited DNA damage to HUVECs, which repairs quickly. HBO treatment protects against H(2)O(2)-induced DNA damage and involves cellular glutathione.
  
  
  CONCLUSIONS
  Endothelial cells are unlikely to be compromised during HBO therapy.
authors.affiliation: School of Biomedical and Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
authors.forename: J
authors.initials: J
authors.lastname: Yuan
authors.forename: R D
authors.initials: RD
authors.lastname: Handy
authors.forename: A J
authors.initials: AJ
authors.lastname: Moody
authors.forename: G
authors.initials: G
authors.lastname: Smerdon
authors.forename: P
authors.initials: P
authors.lastname: Bryson
mesh_terms: D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D016877:Oxidants; D004247:DNA; D006861:Hydrogen Peroxide; D005978:Glutathione; D010100:Oxygen; D019803:Glutathione Disulfide