title: Multiple biomarkers responses in juvenile rainbow trout, Oncorhynchus mykiss, after acute exposure to a fungicide propiconazole.
abstract: In this study, the toxic effects of propiconazole (PCZ), a triazole fungicide present in aquatic environment, were studied in juvenile rainbow trout, Oncorhynchus mykiss, by acute toxicity test with the concentration of 5.04 mg/L (96 h LC50). Morphological indices, hematological parameters, liver xenobiotic-metabolizing response, and tissue antioxidant status were evaluated. Compared with the control group, fish exposed to PCZ showed significantly higher Leuko, PCV, MCHC, and hepatic EROD, and significantly lower MCV. CF and HSI were not significantly different among groups. SOD, CAT, GPx, and GR activities increased significantly in liver of experimental groups, but decreased significantly in gill. In general, antioxidant enzyme activity in intestine was less evident than in liver. Oxidative stress indices (levels of LPO and CP) were significantly higher in gill. Additionally, through chemometrics of all parameters measured in this study, two groups with 67.29% of total accumulated variance were distinguished. In short, the physiological and biochemical responses in different tissues of fish indicated that PCZ-induced the stressful environmental conditions. But according to PCZ residual status in the natural environment, more long-term experiments at lower concentrations will be necessary in the future. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.
authors.affiliation: South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic. zhihuali06@yahoo.com
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zlabek
authors.forename: Josef
authors.initials: J
authors.lastname: Velisek
authors.forename: Roman
authors.initials: R
authors.lastname: Grabic
authors.forename: Jana
authors.initials: J
authors.lastname: Machova
authors.forename: Jitka
authors.initials: J
authors.lastname: Kolarova
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Tomas
authors.initials: T
authors.lastname: Randak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D005659:Fungicides, Industrial / Q000633:toxicity*; D005880:Gills / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D023382:Toxicity Tests, Acute; D014230:Triazoles / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005659:Fungicides, Industrial; D014230:Triazoles; D014874:Water Pollutants, Chemical; C045950:propiconazole; D019363:Cytochrome P-450 CYP1A1