title: A rapid and sensitive liquid chromatography-tandem mass spectrometry method for determination of olopatadine concentration in human plasma.
abstract: A sensitive and rapid method based on liquid chromatography- tandem mass spectrometry (MS-MS) was developed for the determination of olopatadine in human plasma. Sample preparations were carried out by protein precipitation with the addition of acetonitrile followed by liquid-liquid extraction with ethyl acetate/dichloromethane after internal standard (IS, amitriptyline) spiked. After evaporation to dryness, the resultant residue was reconstituted in mobile phase. Separation of olopatadine and IS from the interferences was achieved on a C(18) column followed by MS-MS detection. The analytes were monitored in the positive ionization mode with a TurboIonspray source. Multiple reaction monitoring using the transition of m/z 338 → 165 and m/z 278 → 91 was performed to quantify olopatadine and IS, respectively. The method had a total chromatographic run time of 3.5 min and linear calibration curves over the concentration range of 0.2-100 ng/mL. The lower limit of quantification was 0.2 ng/mL. For each QC concentration level the intra- and interday precisions were less than 11.4%, and relative errors ranged between -6.40% and 9.26%. The validated method was successfully applied to the quantification of olopatadine concentration in human plasma after administration of olopatadine at an oral dose of 5 mg in order to evaluate the pharmacokinetics.
authors.affiliation: Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, and Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Yu-Guan
authors.initials: YG
authors.lastname: Wen
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Fan
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhou
authors.forename: Rui-Xin
authors.initials: RX
authors.lastname: Fan
authors.forename: Ji-Mei
authors.initials: JM
authors.lastname: Chen
authors.forename: Ke-Li
authors.initials: KL
authors.lastname: Huang
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Zhu
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Zhuang
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003990:Dibenzoxepins / Q000097:blood*; D006801:Humans; D008297:Male; D000069605:Olopatadine Hydrochloride; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003990:Dibenzoxepins; D000069605:Olopatadine Hydrochloride