title: Agitation-free multiphoton microscopy of oblique planes.
abstract: The scanning two-photon fluorescence microscope produces optically sectioned images from the focal plane. It is sometimes desirable to acquire images from other planes of the specimen that are inclined with respect to the focal plane. In this Letter, we discuss the issues concerned with acquiring such images together with the effects of the inclination angle on image resolution and sectioning strength. To obtain images from oblique planes at high speed, a two-photon system was built wherein a novel optical system is used to provide aberration-free scanning.
authors.affiliation: Department of Engineering Science, University of Oxford, Oxford, UK.
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Smith
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Botcherby
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Booth
authors.forename: Rimas
authors.initials: R
authors.lastname: Juškaitis
authors.forename: Tony
authors.initials: T
authors.lastname: Wilson
mesh_terms: D008853:Microscopy / Q000379:methods*; D017785:Photons; D011058:Pollen; D012399:Rotation