title: Changed histone acetylation patterns in normal-appearing white matter and early multiple sclerosis lesions.
abstract: The epigenetic identity of oligodendrocytes is modulated by posttranslational modifications of histones. Acetylation of histone H3 results from the balance between the activity of histone acetyltransferases (HATs) and histone deacetylases and modulates transcriptional activation. We have previously shown that, in rodents, histone deacetylation favors oligodendrocyte differentiation, whereas acetylation is associated with increased levels of transcriptional inhibitors of oligodendrocyte differentiation. Here, we report, in humans brains, a shift toward histone acetylation in the white matter of the frontal lobes of aged subjects and in patients with chronic multiple sclerosis (MS). Increased immunoreactivity for acetylated histone H3 was observed in the nuclei of NogoA+ oligodendrocytes in a subset of MS samples. These changes were associated with high levels of transcriptional inhibitors of oligodendrocyte differentiation (i.e., TCF7L2, ID2, and SOX2) and higher HAT transcript levels (i.e., CBP, P300) in female MS patients compared with non-neurological controls and correlated with disease duration. Chromatin immunoprecipitation from samples of MS patients revealed enrichment of acetyl-histone H3 at the promoter of the increased target genes (i.e., TCF7L2). The data in chronic lesions contrasted with findings in early MS lesions, where a marked oligodendroglial histone deacetylation was observed. Together, these data suggest that histone deacetylation is a process that occurs at the early stages of the disease and whose efficiency decreases with disease duration.
authors.affiliation: Department of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: Xiomara
authors.initials: X
authors.lastname: Pedre
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Mastronardi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Bruck
authors.forename: Gerardo
authors.initials: G
authors.lastname: López-Rodas
authors.forename: Tanja
authors.initials: T
authors.lastname: Kuhlmann
authors.forename: Patrizia
authors.initials: P
authors.lastname: Casaccia
mesh_terms: D000107:Acetylation; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D042241:Early Diagnosis; D005260:Female; D051548:Histone Acetyltransferases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D009413:Nerve Fibers, Myelinated / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D055815:Young Adult
chemical_list: D006657:Histones; D051548:Histone Acetyltransferases