title: Widespread, focal copy number variations (CNV) and whole chromosome aneuploidies in Trypanosoma cruzi strains revealed by array comparative genomic hybridization.
abstract: BACKGROUND
  Trypanosoma cruzi is a protozoan parasite and the etiologic agent of Chagas disease, an important public health problem in Latin America. T. cruzi is diploid, almost exclusively asexual, and displays an extraordinarily diverse population structure both genetically and phenotypically. Yet, to date the genotypic diversity of T. cruzi and its relationship, if any, to biological diversity have not been studied at the whole genome level.
  
  
  RESULTS
  In this study, we used whole genome oligonucleotide tiling arrays to compare gene content in biologically disparate T. cruzi strains by comparative genomic hybridization (CGH). We observed that T. cruzi strains display widespread and focal copy number variations (CNV) and a substantially greater level of diversity than can be adequately defined by the current genetic typing methods. As expected, CNV were particularly frequent in gene family-rich regions containing mucins and trans-sialidases but were also evident in core genes. Gene groups that showed little variation in copy numbers among the strains tested included those encoding protein kinases and ribosomal proteins, suggesting these loci were less permissive to CNV. Moreover, frequent variation in chromosome copy numbers were observed, and chromosome-specific CNV signatures were shared by genetically divergent T. cruzi strains.
  
  
  CONCLUSIONS
  The large number of CNV, over 4,000, reported here uphold at a whole genome level the long held paradigm of extraordinary genome plasticity among T. cruzi strains. Moreover, the fact that these heritable markers do not parse T. cruzi strains along the same lines as traditional typing methods is strongly suggestive of genetic exchange playing a major role in T. cruzi population structure and biology.
authors.affiliation: Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Todd A
authors.initials: TA
authors.lastname: Minning
authors.forename: D Brent
authors.initials: DB
authors.lastname: Weatherly
authors.forename: Stephane
authors.initials: S
authors.lastname: Flibotte
authors.forename: Rick L
authors.initials: RL
authors.lastname: Tarleton
mesh_terms: D000782:Aneuploidy; D055028:Comparative Genomic Hybridization / Q000379:methods*; D056915:DNA Copy Number Variations; D016054:DNA, Protozoan / Q000235:genetics; D014644:Genetic Variation; D018503:Genome, Protozoan; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan