title: Value of Golgi protein 73 monoclonal antibody in diagnosis of hepatocellular carcinoma.
abstract: OBJECTIVE
  To explore the sensitivity and specificity of Golgi protein 73 (GP73) monoclonal antibody in the diagnosis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  Self-prepared GP73 monoclonal antibody was used as the primary antibody for detecting the serum GP73 levels in healthy controls(n=31)and HCC patients (n=59). The baseline level of the healthy controls was determined by semiquantitative analysis. The results were compared with those from GP73 polyclonal antibody and alpha-fetoprotein (AFP).
  
  
  RESULTS
  The GP73 level of healthy controls was 1.2 (0.9-1.7) relative unit (RU), which was significantly lower than that of HCC patients [5.7 (2.5-7.8) RU] (P<0.001) with monoclonal antibody. Using polyclonal antibody, the GP73 level of HCC patients was also significantly higher than healthy controls [7.8 (3.0-12.4) RU vs. 1.1 (1.0-2.0) RU, P<0.001]. The sensitivity and specificity of GP73 monoclonal antibody in diagnosis of HCC were 84.7% and 93.5%; on the contrary, those of GP73 polyclonal antibody were 78.0% and 93.5%, respectively. The sensitivity and specificity of AFP (67.8% and 74.2%, respectively) in the HCC patients were markedly lower than those of GP73. Logistic regression analysis showed that the odds ratio (OR) of GP73 monoclonal antibody was 7.18 and that of GP73 polyclonal antibody was 1.51.
  
  
  CONCLUSIONS
  Our self-prepared monoclonal antibody can effectively detect GP73 serum level in HCC patients, and has higher sensitivity and specificity than AFP. It may be superior to the currently used GP73 polyclonal antibody. The results lay the foundation for the further development of ELISA methods by using this monoclonal antibody.
authors.affiliation: Department of Liver Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Hua-yu
authors.forename: Mao
authors.initials: M
authors.lastname: Yi-lei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jin-chun
authors.forename: Xu
authors.initials: X
authors.lastname: Hai-feng
authors.forename: Jiang
authors.initials: J
authors.lastname: Gui-yong
authors.forename: Jing
authors.initials: J
authors.lastname: Yan-ling
authors.forename: Chen
authors.initials: C
authors.lastname: Xin-xin
authors.forename: Lu
authors.initials: L
authors.lastname: Xin
authors.forename: Sang
authors.initials: S
authors.lastname: Xin-ting
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hong-bing
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008565:Membrane Proteins / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; C410824:GOLM1 protein, human; D008565:Membrane Proteins