title: [Peculiarities of ethanol oxidation by the producer of surface-active substances Acinetobacter calcoaceticus K-4].
abstract: Activity of key-enzymes of C2-metabolism was determined in the cells of strain-producer of surface-active substances Acinetobacter calcoaceticus K-4 grown on ethanol. It is shown that ethanol and acetaldehyde oxidation in the strain K-4 is performed by pyroquinolinquinon (PQQ) and 4-nitroso-N,N-dimethylaniline (NDMA)-dependent dehydrogenases. Activity of NDMA-dependent enzymes was maximum (100-300 nmol min(-1) mg(-1) of protein) in the early exponential growth phase of A. calcoaceticus K-4. Availability of NDMA-dependent alcohol and acetaldehyde dehydrogenases in gram-negative bacteria was established for the first time. Acetate is involved in metabolism in the strain K-4 with participation of both acetate kinase and acetate-KoA-synthetase; replenishment of the pool of C4-dicarbonic acids is performed in glioxylate cycle (activity of isocytrate lyase is 600 nmol min(-1) mg(-1) of protein) and in phosphoenolpyruvate carboxylase reaction (1600 nmol min(-1) mg(-1) of protein). Both key enzymes of gluconeogenesis take place in synthesis of carbohydrates: FEP-carboxykinase and FEP-synthetase (1200 and 4400 nmol min(-1) mg(-1) of protein, respectively). Enzymatic investigations have confirmed the capacity of strain K-4 to synthesis of surface-active trehalosemycolates (activity of trehalosephosphate synthase is 150-160 nmol min(-1) mg(-1) of protein).
authors.forename: T P
authors.initials: TP
authors.lastname: Pyroh
authors.forename: T A
authors.initials: TA
authors.lastname: Shevchuk
authors.forename: O S
authors.initials: OS
authors.lastname: Duhinets'
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D013501:Surface-Active Agents; D000431:Ethanol; D000429:Alcohol Oxidoreductases; C105563:alcohol - NDMA oxidoreductase