title: Multilocus microsatellite typing revealed high genetic variability of Leishmania donovani strains isolated during and after a Kala-azar epidemic in Libo Kemkem district, northwest Ethiopia.
abstract: In 2004, an outbreak of kala-azar (KA) occurred for the first time in Libo Kemkem district, in the highland area of northwest Ethiopia. In order to track the possible origins of the outbreak parasites, we have investigated 19 strains of Leishmania donovani that were collected during (n = 6) and after (n = 13) the outbreak by using 14 highly polymorphic microsatellite markers. Unique microsatellite profiles were obtained for all strains from Libo Kemkem. When compared to those of L. donovani strains from different Ethiopian, Kenyan and Sudanese foci, by genetic distance and Bayesian clustering model analyses, most strains from Libo Kemkem grouped with strains from: (i) Humera and Metema in the lowlands and Belessa in the highland of Ethiopia, and (ii) Sudan, at different hierarchal levels. The strains from Libo Kemkem district were assigned at least to three genetically distinct clusters (A, B1 and B2) of which only one, cluster B2, consisted exclusively of strains from Libo Kemkem. The fact that most of the outbreak strains were found to be related to strains from well-known KA foci in northwest Ethiopia and Sudan might suggest multiple introductions of L. donovani strains from these foci into Libo Kemkem district.
authors.affiliation: Institut für Mikrobiologie und Hygiene, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany. tesfayegela2002@yahoo.com
authors.forename: Tesfaye
authors.initials: T
authors.lastname: Gelanew
authors.forename: Israel
authors.initials: I
authors.lastname: Cruz
authors.forename: Katrin
authors.initials: K
authors.lastname: Kuhls
authors.forename: Jorge
authors.initials: J
authors.lastname: Alvar
authors.forename: Carmen
authors.initials: C
authors.lastname: Cañavate
authors.forename: Asrat
authors.initials: A
authors.lastname: Hailu
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schönian
mesh_terms: D016000:Cluster Analysis; D004196:Disease Outbreaks; D005002:Ethiopia / Q000453:epidemiology; D014644:Genetic Variation; D006801:Humans; D007893:Leishmania donovani / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000453:epidemiology*; D018895:Microsatellite Repeats; D017720:Molecular Epidemiology; D058889:Molecular Typing