title: The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs.
abstract: This study describes the expression of heat shock protein70 (HSP70) and alpha-basic-crystallin (α-BC) and their association with apoptosis and some related adaptor proteins in the pathogenesis of foot-and-mouth disease virus (FMDV)-induced myocarditis in lambs. HSP70 was generally overexpressed in the myocardial tissues and inflammatory cells of FMDV-induced myocarditis with differential accumulation and localization in same hearts when compared to non-foot-and-mouth disease control hearts. α-BC immunolabeling showed coarse aggregations in the Z line of the cardiomyocytes in FMDV-infected hearts in contrast to control hearts. Overall, the results of this study show that the anti-apoptotic proteins, HSP70 and α-BC, were overexpressed with increased apoptosis in FMDV-infected heart tissues. Both proteins failed to protect the cardiomyocytes from apoptosis as defense mechanisms to the FMDV during the infection, suggesting that the virus is able to increase apoptosis via both downregulation and/or upregulation of these anti-apoptotic proteins.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey. myg64@omu.edu.tr
authors.forename: Mustafa Yavuz
authors.initials: MY
authors.lastname: Gulbahar
authors.forename: Yonca Betil
authors.initials: YB
authors.lastname: Kabak
authors.forename: Mehmet Onder
authors.initials: MO
authors.lastname: Karayigit
authors.forename: Murat
authors.initials: M
authors.lastname: Yarim
authors.forename: Tolga
authors.initials: T
authors.lastname: Guvenc
authors.forename: Unal
authors.initials: U
authors.lastname: Parlak
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D005536:Foot-and-Mouth Disease / Q000150:complications* / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification*; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D009205:Myocarditis / Q000150:complications / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D009206:Myocardium / Q000473:pathology; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D014421:Turkey; D038203:alpha-Crystallin B Chain / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D018840:HSP70 Heat-Shock Proteins; D038203:alpha-Crystallin B Chain