title: The effect of olive oil polyphenols on antibodies against oxidized LDL. A randomized clinical trial.
abstract: OBJECTIVE
  Oxidized LDL (oxLDL) is a highly immunogenic particle that plays a key role in the development of atherosclerosis. Some data suggest a protective role of OxLDL autoantibodies (OLAB) in atherosclerosis. Our aim was to assess the effect of olive oil polyphenols on the immunogenicity of oxLDL to autoantibody generation.
  
  
  METHODS
  In a crossover, controlled trial, 200 healthy men were randomly assigned to 3-week sequences of 25 mL/day of 3 olive oils with high (366 mg/kg), medium (164 mg/kg), and low (2.7 mg/kg) phenolic content.
  
  
  RESULTS
  Plasma OLAB concentration was inversely associated with oxLDL (p < 0.001). Olive oil phenolic content increased OLAB generation, with the effect being stronger at higher concentrations of oxLDL (p = 0.020 for interaction). A direct relationship was observed between OLAB and the total olive oil phenol content in LDL (r = 0.209; p = 0.014). OLAB concentrations, adjusted for oxLDL, increased directly in a dose-dependent manner with the polyphenol content of the olive oil administered (p = 0.023).
  
  
  CONCLUSIONS
  Olive oil polyphenols promote OLAB generation. This effect is stronger at higher concentrations of lipid oxidative damage.
authors.affiliation: Cardiovascular Risk and Nutrition, Cardiovascular Epidemiology and Genetics, and Human Pharmacology and Clinical Neurosciences Research Groups, Research Institute Hospital del Mar (IMIM), Barcelona 08003, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Castañer
authors.forename: Montserrat
authors.initials: M
authors.lastname: Fitó
authors.forename: M Carmen
authors.initials: MC
authors.lastname: López-Sabater
authors.forename: Henrik E
authors.initials: HE
authors.lastname: Poulsen
authors.forename: Kristiina
authors.initials: K
authors.lastname: Nyyssönen
authors.forename: Helmut
authors.initials: H
authors.lastname: Schröder
authors.forename: Jukka T
authors.initials: JT
authors.lastname: Salonen
authors.forename: Karina
authors.initials: K
authors.lastname: De la Torre-Carbot
authors.forename: Hans-Franz
authors.initials: HF
authors.lastname: Zunft
authors.forename: Rafael
authors.initials: R
authors.lastname: De la Torre
authors.forename: Hans
authors.initials: H
authors.lastname: Bäumler
authors.forename: Antonio V
authors.initials: AV
authors.lastname: Gaddi
authors.forename: Guillermo T
authors.initials: GT
authors.lastname: Saez
authors.forename: Marta
authors.initials: M
authors.lastname: Tomás
authors.forename: Maria-Isabel
authors.initials: MI
authors.lastname: Covas
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D006801:Humans; D016014:Linear Models; D015227:Lipid Peroxidation / Q000276:immunology; D008077:Lipoproteins, LDL / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D000069463:Olive Oil; D018384:Oxidative Stress; D010938:Plant Oils / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D008077:Lipoproteins, LDL; D000069463:Olive Oil; D010938:Plant Oils; D059808:Polyphenols; C099252:oxidized low density lipoprotein