title: Amniotic liquid derived stem cells as reservoir of secreted angiogenic factors capable of stimulating neo-arteriogenesis in an ischemic model.
abstract: Most urgent health problems are related to a blood vessel formation failure. The use of stem cells from different sources or species for both in vitro and in vivo engineering of endothelium does not necessarily imply their direct commitment towards a vascular phenotype. In the present study, we used human amniotic fluid stem cells (AFSC) to evoke a strong angiogenic response in murine recipients, in terms of host guided-regeneration of new vessels, and we demonstrated that the AFSC secretome is responsible for the vascularising properties of these cells. We indentified in AFSC conditioned media (ACM) pro-angiogenic soluble factors, such as MCP-1, IL-8, SDF-1, VEGF. Our in vitro results suggest that ACM are cytoprotective, pro-differentiative and chemoattractive for endothelial cells. We also tested ACM on a pre-clinical model of hind-limb ischemic mouse, concluding that ACM contain mediators that promote the neo-arteriogenesis, as remodelling of pre-existing collateral arteries to conductance vessels, thus preventing the capillary loss and the tissue necrosis of distal muscles. In line with the current regenerative medicine trend, in the present study we assert the concept that stem cell-secreted mediators can guide the tissue repair by stimulating or recruiting host reparative cells.
authors.affiliation: Department of Oncology, Biology and Genetics, University of Genoa, Largo Rosanna Benzi 10, 16132 Genova, Italy. teodelinda.mirabella@istge.it
authors.forename: Teodelinda
authors.initials: T
authors.lastname: Mirabella
authors.forename: Mirabella
authors.initials: M
authors.lastname: Teodelinda
authors.forename: Michele
authors.initials: M
authors.lastname: Cilli
authors.forename: Cilli
authors.initials: C
authors.lastname: Michele
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Carlone
authors.forename: Carlone
authors.initials: C
authors.lastname: Sebastiano
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
authors.forename: Cancedda
authors.initials: C
authors.lastname: Ranieri
authors.forename: Chiara
authors.initials: C
authors.lastname: Gentili
authors.forename: Gentili
authors.initials: G
authors.lastname: Chiara
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage* / Q000378:metabolism; D000818:Animals; D001158:Arteries / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005121:Extremities / Q000098:blood supply; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D043925:Angiogenesis Inducing Agents