title: Analysis of white spot syndrome virus envelope protein complexome by two-dimensional blue native/SDS PAGE combined with mass spectrometry.
abstract: White spot syndrome virus (WSSV) is a large enveloped virus, but the organization of its envelope proteins remains largely unknown. In the present study, we used blue native polyacrylamide gel electrophoresis (BN-PAGE) and SDS-PAGE in combination with mass spectrometry to analyze the envelope protein complexome of WSSV. Our results show that the viral envelope consists of multi-protein complexes (MPCs). Within them, the envelope protein VP19 exists as a homotrimer, while another major envelope protein, VP28, mainly exists as a homotetramer. The most notable feature is that the majority of MPCs include VP26 and VP24, suggesting that these two proteins might serve as hub proteins to recruit low-abundance proteins to MPCs and play crucial roles in the process of protein complex formation. Furthermore, we found significant evidence for interactions between several low-abundance proteins, such as VP52B/VP38/VP33 and VP12/VP150. The result of this study may promote the further research on WSSV envelope assembly.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, 178 Daxue Rd., Xiamen, 361005, People's Republic of China.
authors.forename: Zichong
authors.initials: Z
authors.lastname: Li
authors.forename: Limei
authors.initials: L
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D014759:Viral Envelope Proteins