title: Heterologous expression of endo-1,4-beta-xylanaseC from Phanerochaete chrysosporium in Pichia pastoris.
abstract: The cDNA of endo-1,4-β-xylanaseC, isolated from Phanerochaete chrysosporium, was expressed in Pichia pastoris, under the control of the alcohol oxidase I promoter. Using either the intrinsic leader peptide of xylanaseC or the α-factor signal peptide of Saccharomyces cerevisiae, xylanaseC is efficiently secreted into the medium, at a maximum concentration of 2500 U·l(-1).
authors.affiliation: Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Republic of Korea.
authors.forename: Nguyen Duc
authors.initials: ND
authors.lastname: Huy
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Seung-Moon
authors.initials: SM
authors.lastname: Park
mesh_terms: D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis; D005822:Genetic Vectors; D007218:Industrial Microbiology; D008970:Molecular Weight; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D010843:Pichia / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D043364:Endo-1,4-beta Xylanases