title: Single-wavelength functional photoacoustic microscopy in biological tissue.
abstract: Recently, we developed a reflection-mode relaxation photoacoustic microscope, based on saturation intensity, to measure picosecond relaxation times using a nanosecond laser. Here, using the different relaxation times of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, the oxygen saturation was quantified in vivo with single-wavelength photoacoustic microscopy. All previous functional photoacoustic microscopy measurements required imaging with multiple-laser-wavelength measurements to quantify oxygen saturation. Eliminating the need for multiwavelength measurements removes the influence of spectral properties on oxygenation calculations and improves the portability and cost-effectiveness of functional or molecular photoacoustic microscopy.
authors.affiliation: Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
authors.forename: Amos
authors.initials: A
authors.lastname: Danielli
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Favazza
authors.forename: Konstantin
authors.initials: K
authors.lastname: Maslov
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D004423:Ear; D006454:Hemoglobins / Q000378:metabolism; D051379:Mice; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D010100:Oxygen