title: Effect of alcohol consumption on whole-body protein turnover in healthy adults.
abstract: The aim of the study was to investigate the whole-body protein turnover, either before or after continuous, moderate ethanol-induced oxidative stress by red wine consumption over a relatively short period in healthy volunteers. Ten healthy adults received an individual regular diet over 20 days. After 10 days, the subjects consumed 0.4 ml ethanol kg(-1) day(-1) as red wine together with dinner over a 10-day period. After 8 and 18 days, respectively, a (15)N-labelled yeast protein was administered in a dosage of 4.2 mg kg(-1) body weight. Urine and faeces were collected over 48 h, respectively. The (15)N-enrichment was measured by isotope ratio mass spectrometry, whereas the protein flux rates were calculated by a three-compartment model. The whole-body protein turnover without/with red wine consumption amounted to 3.73±0.6 and 3.49±0.6 g kg(-1) day(-1) (not significant), respectively. Moderate alcohol consumption does not induce significant short-term changes in the whole-body protein turnover of healthy adults.
authors.affiliation: Children's Hospital, Research Laboratory Gastroenterology and Nutrition, University of Rostock, Rostock, Germany. klaus-dieter.wutzke@med.uni-rostock.de
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Wutzke
authors.forename: Helga
authors.initials: H
authors.lastname: Krentz
authors.forename: Gerrit
authors.initials: G
authors.lastname: Bruns
mesh_terms: D000328:Adult; D000428:Alcohol Drinking; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005858:Germany; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes; D018384:Oxidative Stress / Q000187:drug effects; D011506:Proteins / Q000187:drug effects / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D014920:Wine; D055815:Young Adult
chemical_list: D009587:Nitrogen Isotopes; D011506:Proteins; D000431:Ethanol