title: Terahertz radiation induces spindle disturbances in human-hamster hybrid cells.
abstract: The aim of this study was to investigate and quantify the production of spindle disturbances in A(L) cells, a human-hamster hybrid cell line, by 0.106 THz radiation (continuous wave). Monolayer cultures in petri dishes were exposed for 0.5 h to 0.106 THz radiation with power densities ranging from 0.043 mW/cm(2) to 4.3 mW/cm(2) or were kept under sham conditions (negative control) for the same period. As a positive control, 100 µg/ml of the insecticide trichlorfon, which is an aneuploidy-inducing agent, was used for an exposure period of 6 h. During exposure, the sample containers were kept at defined environmental conditions in a modified incubator as required by the cells. Based on a total of 6,365 analyzed mitotic cells, the results of two replicate experiments suggest that 0.106 THz radiation is a spindle-acting agent as predominately indicated by the appearance of spindle disturbances at the anaphase and telophase (especially lagging and non-disjunction of single chromosomes) of cell divisions. The findings in the present study do not necessarily imply disease or injury but may be important for evaluating possible underlying mechanisms.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Universität Würzburg, Germany. Hintzsche@toxi.uni-wuerzburg.de
authors.forename: H
authors.initials: H
authors.lastname: Hintzsche
authors.forename: C
authors.initials: C
authors.lastname: Jastrow
authors.forename: T
authors.initials: T
authors.lastname: Kleine-Ostmann
authors.forename: H
authors.initials: H
authors.lastname: Stopper
authors.forename: E
authors.initials: E
authors.lastname: Schmid
authors.forename: T
authors.initials: T
authors.lastname: Schrader
mesh_terms: D000705:Anaphase / Q000528:radiation effects; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000528:radiation effects*; D013692:Telophase / Q000528:radiation effects; D013997:Time Factors