title: Cell investigations simultaneously with exposure to 2.45 GHz microwaves.
abstract: The paper presents two microwave (MW) exposure systems (MWESs) that permit observations and measurements on cell cultures during their exposure to MW of 2.45 GHz: MWES-1 and MWES-2. MWES-1 is designed for the measurement of the cell membrane fluorescence anisotropies (MFA) simultaneously with MW exposure. MWES-2 is designed for the cells culture exploration under an inverted microscope before, during and after MW exposure. MWES-1 consists mainly of a 2.45 GHz microwave generator (MWG-2.45 GHz-SAIREM) of 0-25 W, equipped with forward power and reflected power displaying, and an adjustable coaxial antenna immersed directly into the cuvette with the cells-suspension of a Spex type spectrofluorometer. The MW effect on membrane fluidity of B16F10 malignant melanoma (B16F10-MM) cells in suspension were investigated with MWES-1, by MFA measurements. We observed a MW induced transition temperature (ITT) rising strongly during the MW exposure as compared with ITT obtained by classical heating (CH). The MWES-2 consists of the MWG-2.45 GHz-SAIREM generator and a rectangular waveguide applicator with traveling wave placed between the condenser and the objective of a Zeiss Axiovert 200 microscope, equipped with a fluorescence device and image acquisition. The MW effects on shape and apoptosis of the B16F10-MM cells were investigate with MWES-2. The B16F10-MM cells exhibited visible shape changes during MW exposure up to 37 degrees C. The MW exposure induced cells apoptosis/necrosis in several seconds after that MW are applied, beginning with SAR = 1.5 W/sample, compared to CH controls exposed at the same temperature dynamics.
authors.affiliation: Institute for Lasers, Plasma and Radiation Physics, Bucharest, Romania. diana.martin@inflpr.ro
authors.forename: Diana
authors.initials: D
authors.lastname: Martin
authors.forename: Sabin
authors.initials: S
authors.lastname: Cinca
authors.forename: Irina
authors.initials: I
authors.lastname: Margaritescu
authors.forename: Monica
authors.initials: M
authors.lastname: Neagu
authors.forename: Nicusor
authors.initials: N
authors.lastname: Iacob
authors.forename: Daniel
authors.initials: D
authors.lastname: Ighigeanu
authors.forename: Constantin
authors.initials: C
authors.lastname: Matei
authors.forename: Gabriela
authors.initials: G
authors.lastname: Craciun
authors.forename: Elena
authors.initials: E
authors.lastname: Manaila
authors.forename: Doru Aurel
authors.initials: DA
authors.lastname: Chirita
authors.forename: Mihaela
authors.initials: M
authors.lastname: Moisescu
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D048430:Cell Shape; D004867:Equipment Design; D005454:Fluorescence Polarization / Q000295:instrumentation; D008546:Melanoma, Experimental / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008560:Membrane Fluidity; D051379:Mice; D008872:Microwaves / Q000627:therapeutic use*; D013696:Temperature