title: Binary HPLC-diode array detector and HPLC-evaporative light-scattering detector fingerprints of methanol extracts from the selected sage (Salvia) species.
abstract: This study is focused on an important family of the sage (Salvia) species, with Salvia officinalis L. having a long-established position in European traditional medicine. Binary fingerprints (chromatographic profiles) of six different sage species were compared using HPLC coupled with two different detectors: the diode-array detector and the evaporative light-scattering detector. Advantages of using binary fingerprinting over single-detector fingerprinting are demonstrated and discussed, with selected examples. Experimental data are provided for a comparison of the chemical composition of sage samples originating from two harvesting seasons (2007 and 2008). A number of phytochemical standards (i.e., certain phenolic acids, flavonoids, and coumarin) were used that allowed identification and semiquantitative estimation of these particular compounds in the analyzed methanol extracts.
authors.affiliation: University of Silesia, Institute of Chemistry, 9 Szkolna St, Katowice, Poland.
authors.forename: Mieczysław
authors.initials: M
authors.lastname: Sajewicz
authors.forename: Dorota
authors.initials: D
authors.lastname: Staszek
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Wojtal
authors.forename: Teresa
authors.initials: T
authors.lastname: Kowalska
authors.forename: Michał Ł
authors.initials: MŁ
authors.lastname: Hajnos
authors.forename: Monika
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
mesh_terms: D001892:Camphanes; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D003374:Coumarins / Q000032:analysis; D004365:Drugs, Chinese Herbal; D005419:Flavonoids / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis; D008027:Light; D000432:Methanol; D053723:Panax notoginseng; D010936:Plant Extracts / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*; D027561:Salvia miltiorrhiza; D027543:Salvia officinalis / Q000737:chemistry; D012542:Scattering, Radiation; D013045:Species Specificity
chemical_list: D001892:Camphanes; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C000604051:danshen dripping pill; C017616:phenolic acid; D000432:Methanol