title: Investigation of gene expression of MMP-2 and TIMP-2 mRNA in rat lung in inhaled nickel oxide and titanium dioxide nanoparticles.
abstract: In order to investigate whether or not dispersed nanoparticles have an effect of inflammation and fibrosis on animals, we developed a nanoparticle generation system and examined the gene expression of matrix metalloproteinase (MMP) and tissue inhibitor matrix proteinase (TIMP) in rat lung containing inhaled nickel oxide (NiO) or titanium dioxide (TiO(2)) nanoparticles. In both experiments, Wistar male rats were exposed to NiO or TiO(2) nanoparticles for 4 wk (6 h/day). The geometric mean diameters of NiO and TiO(2) in the chamber were 139 ± 12 nm and 51 ± 9 nm, respectively. The average concentration of the particle number of NiO and TiO(2) was 1.0E+05 /cm(3) and 2.8E+05 /cm(3), respectively. At 4 d, 1 and 3 months after the end of the inhalation, the rats exposed to these particles were sacrificed and the gene expressions of MMP-2, TIMP-2 and type I collagen were measured using RT-PCR. Pathological finding revealed that there was minimum inflammation with nickel oxide only at 4 d and no change with titanium oxide. However, there were no changes of the gene expression of MMP-2, TIMP-2, and type I collagen in either the NiO or TiO(2) exposure groups. In this study, inhalation of nickel oxide and titanium dioxide nanoparticles did not induce the gene expression of MMP-2 and TIMP-2 mRNA in rat lungs.
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan. yasuom@med.uoeh-u.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Takako
authors.initials: T
authors.lastname: Oyabu
authors.forename: Akira
authors.initials: A
authors.lastname: Ogami
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Myojo
authors.forename: Etsushi
authors.initials: E
authors.lastname: Kuroda
authors.forename: Masami
authors.initials: M
authors.lastname: Hirohashi
authors.forename: Manabu
authors.initials: M
authors.lastname: Shimada
authors.forename: Wuled
authors.initials: W
authors.lastname: Lenggoro
authors.forename: Kikuo
authors.initials: K
authors.lastname: Okuyama
authors.forename: Isamu
authors.initials: I
authors.lastname: Tanaka
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects; D000818:Animals; D024042:Collagen Type I; D004396:Coloring Agents / Q000633:toxicity; D004195:Disease Models, Animal; D005109:Extracellular Matrix; D015870:Gene Expression / Q000235:genetics; D019570:Inhalation Exposure / Q000009:adverse effects*; D008168:Lung / Q000201:enzymology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity; D009532:Nickel / Q000633:toxicity; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics* / Q000378:metabolism; D014025:Titanium / Q000633:toxicity
chemical_list: D000393:Air Pollutants; D024042:Collagen Type I; D004396:Coloring Agents; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; C009495:titanium dioxide; D009532:Nickel; C028007:nickel monoxide; D014025:Titanium; D020778:Matrix Metalloproteinase 2