title: RNA interference in Nilaparvata lugens (Homoptera: Delphacidae) based on dsRNA ingestion.
abstract: BACKGROUND
  An efficient and convenient RNA interference (RNAi) technique involving double-stranded RNA (dsRNA) ingestion is useful for gene function studies of non-model insects.
  
  
  RESULTS
  Three dsRNAs targeting different sites within a gene encoding vacuolar ATP synthase subunit E (V-ATPase-E, 21E01) were synthesised for RNAi in Nilaparvata lugens. dsRNA was found to be stable in 0.1 g mL(-1) sucrose solution, but unstable in artificial fodder. Therefore, dsRNAs were orally delivered into N. lugens in 0.1 g mL(-1) sucrose solution. RNAi was induced by all three of the dsRNAs at 0.05 µg µL(-1) in N. lugens. Time dynamics analysis of gene silencing indicated that significant suppression of the target gene began as early as 2 days after ingestion of ds2-21E01 and ds3-21E01. However, significant repressive effects were recorded up to 10 days after exposure to ds1-21E01. The maximum reduction in target gene mRNA was observed after 10 days of treatment, with suppression ratios induced by ds1-21E01, ds2-21E01 and ds3-21E01 of 41, 55 and 48% respectively.
  
  
  CONCLUSIONS
  An efficient and convenient RNAi technique involving dsRNA ingestion has been successfully developed for N. lugens. This will be a useful tool for further functional genomic investigation in this organism.
authors.affiliation: Hubei Insect Resources Utilisation and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Chen
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lin
authors.forename: Tingru
authors.initials: T
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Hua
mesh_terms: D000818:Animals; D001483:Base Sequence; D004435:Eating; D006430:Hemiptera / Q000235:genetics* / Q000502:physiology; D007300:Insect Control / Q000379:methods*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded