title: Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graft.
abstract: BACKGROUND
  Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures.
  
  
  METHODS
  New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones.
  
  
  RESULTS
  The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality.
  
  
  CONCLUSIONS
  The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
authors.affiliation: Department of Experimental Surgery, Catholic University of Córdoba, School of Medicine, Jacinto Rios 571, X5004ASIK Córdoba, Argentina. fernandoalvarez@campus1.uccor.edu.ar
authors.forename: Fernando Andrés
authors.initials: FA
authors.lastname: Alvarez
authors.forename: Gustavo Adolfo
authors.initials: GA
authors.lastname: Colombres
authors.forename: Alejandro
authors.initials: A
authors.lastname: Viqueira
authors.forename: Jorge Eduardo
authors.initials: JE
authors.lastname: Maldonado
authors.forename: Emilio
authors.initials: E
authors.lastname: Alvarellos
authors.forename: Rubén Horacio
authors.initials: RH
authors.lastname: Sambuelli
authors.forename: Teresita
authors.initials: T
authors.lastname: Alvarellos
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D008297:Male; D009852:Omentum / Q000601:surgery*; D016133:Polymerase Chain Reaction; D011817:Rabbits; D013154:Spleen / Q000276:immunology / Q000637:transplantation*; D013156:Splenectomy; D013270:Stomach / Q000601:surgery*; D014182:Transplantation, Autologous