title: A rapid gel electrophoretic chip for serum cholesterol determination.
abstract: We present a rapid gel electrophoretic chip, composed of 2.5% (w/v) acrylamide and 1% (w/v) agarose gel, for serum cholesterol determination using a photo lithography technique. After optimizations, we determined the lipoprotein concentration of standard serum using a conventional enzyme method. The serum was diluted, stained and loaded for 15 min onto the chip. After loading, the intensities of low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) bands separated at the chip were estimated using an image analyzer. The intensities of these bands corresponded to concentrations obtained from a standard enzyme-based method. The detected LDL-C and HDL-C concentrations were linear up to 146 mg dL(-1) and 53 mg dL(-1) respectively. Finally, we carried out the cholesterol analysis using real biological samples obtained from nine volunteers using our electrophoretic chip. The LDL-C and HDL-C levels detected using our chip correlated well with the results obtained using the conventional enzyme-based method r(2) = 0.98 and r(2) = 0.86 for LDL-C and HDL-C, respectively. Although our sample size is small and confined only to health volunteers, we have demonstrated that this proof-of-concept gel electrophoretic chip can determine lipoproteins, simultaneously.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa 923-1292, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Kaminikado
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Ikeda
authors.forename: Koutaro
authors.initials: K
authors.lastname: Idegami
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagatani
authors.forename: Mun'delanji C
authors.initials: MC
authors.lastname: Vestergaard
authors.forename: Masato
authors.initials: M
authors.lastname: Saito
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
mesh_terms: D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D050171:Dyslipidemias / Q000175:diagnosis; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides