title: Intergenotypic variation of nitric oxide and inflammatory markers in preeclampsia: a pilot study in a North Indian population.
abstract: Cytokines appeared to contribute to the development of pathologic condition and eNOS gene polymorphism may affect cytokine production. The aim of this study was to evaluate cytokines pattern in preeclampsia and whether there is any relationship between gene and cytokines production and cytokine with disease severity. This cross-sectional study included 100 women with preeclampsia and 100 healthy pregnant women. Their blood samples were analyzed for nitric oxide (NO), inflammatory cytokines, and eNOS gene polymorphism. Decreased NO and increased cytokine (tumor necrosis factor-α, interleukin-2, and interferon-γ) levels were found in preeclampsia (p < 0.001). Significant differences were found in genotype/allele distribution between the two groups. A significant negative correlation was observed between NO and cytokine levels (tumor necrosis factor-α and interferon-γ) in the preeclamptic group (p = 0.001). We conclude that preeclampsia is associated with decreased levels of NO and increased levels of circulating inflammatory cytokines due to single nucleotide polymorphisms, pointing toward the role of endothelial and inflammatory components.
authors.affiliation: Department of Biochemistry, Lady Hardinge Medical College, New Delhi, India. sharmadeepikalhmc@gmail.com
authors.forename: Deepika
authors.initials: D
authors.lastname: Sharma
authors.forename: Archana
authors.initials: A
authors.lastname: Singh
authors.forename: Shubha Sagar
authors.initials: SS
authors.lastname: Trivedi
authors.forename: Jayashree
authors.initials: J
authors.lastname: Bhattacharjee
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016207:Cytokines / Q000097:blood; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D007249:Inflammation; D009569:Nitric Oxide / Q000097:blood*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism*; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics* / Q000276:immunology* / Q000503:physiopathology; D011247:Pregnancy
chemical_list: D015415:Biomarkers; D016207:Cytokines; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III