title: BPA uptake does not correlate with LAT1 and Ki67 expressions in tumor samples (results of EORTC trial 11001).
abstract: The system L-amino acid transporter (LAT1) imports p-boronophenylalanine (BPA) mainly into proliferating cells. This study investigates in three tumor entities whether the proportion of tumor cells expressing LAT1 and/or Ki67 correlates with BPA uptake. Tumors were analyzed for (10)B concentration with prompt gamma-ray spectroscopy and for Ki67 and LAT1 expressions with immunohistochemical methods. The proportion of LAT1-expressing cells was much higher (5-90%) than that of Ki67-expressing cells (0-20%) and cells expressing both Ki67 and LAT1 (0-5%). Neither LAT1 nor Ki67 expression predicted BPA uptake.
authors.affiliation: Department of Radiotherapy and Radiation Oncology, University Hospital Marburg, Philipps-University Marburg, Marburg, Germany. wittig@med.uni-marburg.de
authors.forename: Andrea
authors.initials: A
authors.lastname: Wittig
authors.forename: Sien-Yi
authors.initials: SY
authors.lastname: Sheu-Grabellus
authors.forename: Laurence
authors.initials: L
authors.lastname: Collette
authors.forename: Raymond
authors.initials: R
authors.lastname: Moss
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Brualla
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Sauerwein
mesh_terms: D001896:Boron Compounds / Q000493:pharmacokinetics*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D013051:Spectrometry, Gamma
chemical_list: D001896:Boron Compounds; D019394:Ki-67 Antigen; D027283:Large Neutral Amino Acid-Transporter 1; D010649:Phenylalanine; C033685:4-boronophenylalanine