title: Novel mutations in a patient isolate of Streptococcus agalactiae with reduced penicillin susceptibility emerging after long-term oral suppressive therapy.
abstract: Penicillin nonsusceptibility has been demonstrated in group B streptococci (GBS), but there is limited information regarding mechanisms of resistance. We report a case of GBS with reduced susceptibility to penicillin emerging after long-term suppressive oral penicillin therapy for a prosthetic joint infection. Molecular characterization of the isolate before and after long-term penicillin therapy revealed 5 mutations in the ligand-binding regions of PBP1a, -2a, and -2x not previously reported in GBS.
authors.affiliation: Centre Hospitalier Universitaire de Québec and OAHPP, 2705 Laurier Blvd., Quebec City, Quebec G1V4G2, Canada. Jean.Longtin@CRCHUQ.ULaval.ca
authors.forename: Jean
authors.initials: J
authors.lastname: Longtin
authors.forename: Christie
authors.initials: C
authors.lastname: Vermeiren
authors.forename: Dea
authors.initials: D
authors.lastname: Shahinas
authors.forename: Gurdip Singh
authors.initials: GS
authors.lastname: Tamber
authors.forename: Allison
authors.initials: A
authors.lastname: McGeer
authors.forename: Donald E
authors.initials: DE
authors.lastname: Low
authors.forename: Kevin
authors.initials: K
authors.lastname: Katz
authors.forename: Dylan R
authors.initials: DR
authors.lastname: Pillai
mesh_terms: D000284:Administration, Oral; D000368:Aged; D003198:Computer Simulation; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010403:Penicillin Resistance; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D010406:Penicillins / Q000008:administration & dosage / Q000494:pharmacology*; D013292:Streptococcus agalactiae / Q000187:drug effects* / Q000235:genetics*
chemical_list: D046915:Penicillin-Binding Proteins; D010406:Penicillins