title: Bacterial transformation (kushner method).
abstract: The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells competent, which simply means they are now able to take up DNA from the solution. After mixing the competent cells with the DNA, they are subjected to a heat shock to promote the uptake of DNA, presumably by affecting the physical state of the lipids in the membrane.
authors.affiliation: Department of Microbiology, University of Surrey, Guildford, Surrey.
authors.forename: J W
authors.initials: JW
authors.lastname: Dale
authors.forename: P J
authors.initials: PJ
authors.lastname: Greenaway