title: How does it act when soluble? Critical evaluation of mechanism of galectin-1 induced T-cell apoptosis.
abstract: Galectin-1 (Gal-1), a mammalian lectin induces apoptosis of T lymphocytes. Contradictory data have resulted in confusing knowledge regarding mechanism of Gal-1 induced T-cell apoptosis. In this paper we aimed to resolve this controversy by comparing cell death induced by low (1.8 μM, lowGal-1) and high (18 μM, highGal-1) concentration of soluble Gal-1. We show that lowGal-1 and highGal-1 trigger phosphatidylserine exposure, generation of rafts and mitochondrial membrane depolarization. In contrast, lowGal-1 but not highGal-1 is dependent on the presence of p56lck and ZAP70 and activates caspase cascade. The results allow the conclusion that the cell-death mechanism strictly depends on the concentration of Gal-1.
authors.affiliation: Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Blaskó
authors.forename: Roberta
authors.initials: R
authors.lastname: Fajka-Boja
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ion
authors.forename: Eva
authors.initials: E
authors.lastname: Monostori
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D020169:Caspases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D037483:Galectin 1 / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
chemical_list: D037483:Galectin 1; D020169:Caspases