title: Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease.
abstract: The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be produced by the participation of both the mantle and hemocytes.
authors.affiliation: IUEM, LEMAR, UMR CNRS/UBO/IRD 6539, Technopole Brest Iroise, 29280 Plouzané, France.
authors.forename: Nolwenn
authors.initials: N
authors.lastname: Trinkler
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bardeau
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marin
authors.forename: Maylis
authors.initials: M
authors.lastname: Labonne
authors.forename: Aurélie
authors.initials: A
authors.lastname: Jolivet
authors.forename: Philippe
authors.initials: P
authors.lastname: Crassous
authors.forename: Christine
authors.initials: C
authors.lastname: Paillard
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000502:physiology* / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman / Q000379:methods; D014733:Vibrio / Q000472:pathogenicity*