title: Dynamics of Pneumocystis carinii air shedding during experimental pneumocystosis.
abstract: To better understand the diffusion of Pneumocystis in the environment, airborne shedding of Pneumocystis carinii in the surrounding air of experimentally infected rats was quantified by means of a real-time polymerase chain reaction assay, in parallel with the kinetics of P. carinii loads in their lungs. P. carinii DNA was detected in the air 1 week after infection and increased until 4-5 weeks after infection before stabilizing. A significant correlation was shown between lung burdens and the corresponding airborne levels, suggesting the possibility of estimating the fungal lung involvement through quantification of Pneumocystis in the exhaled air.
authors.affiliation: Department of Parasitology-Mycology, Paris-Diderot University and Saint Louis Hospital (Assistance Publique-Hôpitaux de Paris), Université Lille--Nord de France, France.
authors.forename: Firas
authors.initials: F
authors.lastname: Choukri
authors.forename: El Moukhtar
authors.initials: el M
authors.lastname: Aliouat
authors.forename: Jean
authors.initials: J
authors.lastname: Menotti
authors.forename: Anne
authors.initials: A
authors.lastname: Totet
authors.forename: Nausicaa
authors.initials: N
authors.lastname: Gantois
authors.forename: Yves J F
authors.initials: YJ
authors.lastname: Garin
authors.forename: Vance
authors.initials: V
authors.lastname: Bergeron
authors.forename: Eduardo
authors.initials: E
authors.lastname: Dei-Cas
authors.forename: Francis
authors.initials: F
authors.lastname: Derouin
mesh_terms: D000391:Air Microbiology; D000818:Animals; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D008168:Lung / Q000382:microbiology; D009172:Mycology / Q000379:methods; D045363:Pneumocystis carinii / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D013997:Time Factors
chemical_list: D004271:DNA, Fungal