title: [Cryopreservation of plasmodia with malaria models and establishment of a cryobank].
abstract: OBJECTIVE
  Cryopreservation is simply a method of keeping living cells frozen with the chance of regaining cellular viability, functions and antigenic structures whenever required, after heating.
  
  
  METHODS
  In the present study, dimethyl sulphoxide (DMSO) was mixed with the red blood cells having 20% of parasitemia obtained from the mice infected with Plasmodium yoelii and Plasmodium berghei at a final concentration of 15%. For cryopreservation: both test tubes containing each Plasmodium species were kept 10 minutes at room temperature, 30 minutes at +4°C, 90 minutes at -20°C and finally at -80°C. Some were left at this temperature, while some were transferred into the liquid nitrogen tank at -196°C after being left at -80°C for three hours.
  
  
  RESULTS
  Our observations and assessments demonstrated that both P. yoelii and P. berghei might keep their viability and virulence at -80°C and -196°C between the first and the sixth months of cryopreservation.
  
  
  CONCLUSIONS
  It can be concluded that the cryopreservation of P. yoelii and P. berghei at -80°C and -196°C are successful, indicating the advantage of the establishment of parasite cryobanks in research laboratories.
authors.affiliation: Celal Bayar Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Manisa, Türkiye. a.ozbilgin@yahoo.com
authors.forename: Ahmet
authors.initials: A
authors.lastname: Özbılgın
authors.forename: İpek
authors.initials: İ
authors.lastname: Östan
authors.forename: Tuba
authors.initials: T
authors.lastname: Tabak
authors.forename: Kamil
authors.initials: K
authors.lastname: Aşar
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods* / Q000592:standards; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000469:parasitology*; D008288:Malaria / Q000097:blood / Q000469:parasitology*; D008297:Male; D051379:Mice; D010962:Plasmodium berghei / Q000502:physiology*; D010967:Plasmodium yoelii / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide