title: NOS2A as a candidate gene in Relapsing-Remitting Multiple Sclerosis: a haplotype study using selected subsets of single nucleotide polymorphisms.
abstract: As with other autoimmune diseases, susceptibility to multiple sclerosis (MS) is believed to result from the complex interaction of a number of genes, each with modest effect. Several genome-wide screens have implicated the nitric oxide synthase 2A gene (NOS2A), which encodes the inducible form of nitric oxide synthase, as being potentially associated with MS. To determine whether genetic variants within this gene may constitute a risk factor for causing MS, we investigated 13 single nucleotide polymorphisms in the NOS2A gene, in a case-control group of 214 Italian patients with MS and 121 controls. All these single nucleotide polymorphisms (SNPs) were analyzed using the SNPlex™ Genotyping System (Applied Biosystems). Data were analyzed using Genemapper 4.0 and Haploview 4.1. No significant association between cases and controls (P>0.05) was observed for the alleles of the SNPs. Defining the haplotype blocks also failed to detect any associated haplotypes. Our results suggest that polymorphic variation within the NOS2A gene does not influence the susceptibility to MS in patients of Italian origin.
authors.affiliation: Institute of Neurological Science, National Research Council, Cosenza, Italy. i.manna@isn.cnr.it
authors.forename: Ida
authors.initials: I
authors.lastname: Manna
authors.forename: Maria
authors.initials: M
authors.lastname: Liguori
authors.forename: Paola
authors.initials: P
authors.lastname: Valentino
authors.forename: Luana
authors.initials: L
authors.lastname: Vena
authors.forename: Francesca
authors.initials: F
authors.lastname: Condino
authors.forename: Rita
authors.initials: R
authors.lastname: Nisticò
authors.forename: Gemma
authors.initials: G
authors.lastname: Di Palma
authors.forename: Aldo
authors.initials: A
authors.lastname: Quattrone
authors.forename: Antonio
authors.initials: A
authors.lastname: Gambardella
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies / Q000379:methods; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008297:Male; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000175:diagnosis / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D055815:Young Adult
chemical_list: C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II