title: [Comparison between old and new methods for detection of allergenic substances (egg and milk)].
abstract: The old ELISA method for detection of allergenic substances (egg and milk) in Kanagawa prefecture from 2003 to 2007, employed before improvement of the food allergen labeling system, yielded detection rates of 20% for egg and 30% for milk. In 2005, after improvement of the labeling system, the detection rate using the new ELISA in solutions containing 1% SDS and 7% 2-mercaptoethanol increased by about 10% for egg, but decreased by half for milk. There were 4 positive samples (over 10 µg/g) for both egg and milk proteins, on account of contamination by ingredients at the manufacturing line and the lack of proper food labeling. In 2009, the contamination levels of egg and milk proteins in labeled commercial foods were low. In a comparison between the new and old methods with the same samples, both the new ELISA and Western-blot analyses showed an increase in the detection rate of egg protein. In relation to milk protein, the detection rates were decreased with the new ELISA, although the ELISA detection rate and consistency rates with Western-blot analysis were increased. On the other hand, in the case of a protein content below 5 µg/g, it was impossible to determine ovomucoid and casein by Western-blot analysis.
authors.affiliation: Chemistry Division, Kanagawa Prefectual Institute of Public Health, Chigasaki-shi, Kanagawa, Japan. Watanabe.jnsd@pref.kanagawa.jp
authors.forename: Hiroko
authors.initials: H
authors.lastname: Watanabe
authors.forename: Chie
authors.initials: C
authors.lastname: Akaboshi
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Saita
authors.forename: Haruko
authors.initials: H
authors.lastname: Sekido
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hashiguchi
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Watabe
authors.forename: Kouki
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000485:Allergens / Q000032:analysis*; D015153:Blotting, Western; D002364:Caseins / Q000032:analysis; D004527:Egg Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008894:Milk Proteins / Q000032:analysis*; D010059:Ovomucin / Q000032:analysis
chemical_list: D000485:Allergens; D002364:Caseins; D004527:Egg Proteins; D008894:Milk Proteins; D010059:Ovomucin