title: Production and Purification of Hybrid Ty-VLPs.
abstract: The self-assembly properties of a protein encoded by the TYA gene of the yeast Ty element can be exploited to produce hybrid Ty-VLPs (virus-like particles) (1,2). There has been developed a series of expression vectors that allow the construction of Ty fusion genes containing protein coding sequences of interest (see Chapter 24 ). Many different hybrid Ty-VLPs have now been produced that carry additional proteins that range in size from 3 to 42 kDa. These include regions from human immunodeficiency virus-1 (HIV-1) env, pol, tat, rev, nef, and vif genes; influenza virus hemagglutinin; human α-interferon, feline leukemia virus env, and bovine papillomavirus El and E2 (1-5 and unpublished data).
authors.affiliation: British Bio-technology Ltd., Oxford, UK.
authors.forename: N R
authors.initials: NR
authors.lastname: Burns
authors.forename: J E
authors.initials: JE
authors.lastname: Gilmour
authors.forename: S M
authors.initials: SM
authors.lastname: Kingsman
authors.forename: A J
authors.initials: AJ
authors.lastname: Kingsman
authors.forename: S E
authors.initials: SE
authors.lastname: Adams