title: Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopreserved cord blood.
abstract: Cryopreservation of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) is crucial for cord blood (CB) banking and transplantation. We evaluated recovery of functional HPC cryopreserved as mononuclear or unseparated cells for up to 23.5 years compared with prefreeze values of the same CB units. Highly efficient recovery (80%-100%) was apparent for granulocyte-macrophage and multipotential hematopoietic progenitors, although some collections had reproducible low recovery. Proliferative potential, response to multiple cytokines, and replating of HPC colonies was extensive. CD34(+) cells isolated from CB cryopreserved for up to 21 years had long-term (≥ 6 month) engrafting capability in primary and secondary immunodeficient mice reflecting recovery of long-term repopulating, self-renewing HSCs. We recovered functionally responsive CD4(+) and CD8(+) T lymphocytes, generated induced pluripotent stem (iPS) cells with differentiation representing all 3 germ cell lineages in vitro and in vivo, and detected high proliferative endothelial colony forming cells, results of relevance to CB biology and banking.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA. hbroxmey@iupui.edu
authors.forename: Hal E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: Man-Ryul
authors.initials: MR
authors.lastname: Lee
authors.forename: Giao
authors.initials: G
authors.lastname: Hangoc
authors.forename: Scott
authors.initials: S
authors.lastname: Cooper
authors.forename: Nutan
authors.initials: N
authors.lastname: Prasain
authors.forename: Young-June
authors.initials: YJ
authors.lastname: Kim
authors.forename: Coleen
authors.initials: C
authors.lastname: Mallett
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Ye
authors.forename: Scott
authors.initials: S
authors.lastname: Witting
authors.forename: Kenneth
authors.initials: K
authors.lastname: Cornetta
authors.forename: Linzhao
authors.initials: L
authors.lastname: Cheng
authors.forename: Mervin C
authors.initials: MC
authors.lastname: Yoder
mesh_terms: D000818:Animals; D001793:Blood Preservation; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D042783:Endothelial Cells / Q000166:cytology; D005312:Fetal Blood / Q000166:cytology* / Q000637:transplantation; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation; D007231:Infant, Newborn; D053631:Interleukin Receptor Common gamma Subunit / Q000172:deficiency / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit