title: PA0305 of Pseudomonas aeruginosa is a quorum quenching acylhomoserine lactone acylase belonging to the Ntn hydrolase superfamily.
abstract: The Pseudomonas aeruginosa PAO1 genome has at least two genes, pvdQ and quiP, encoding acylhomoserine lactone (AHL) acylases. Two additional genes, pa1893 and pa0305, have been predicted to encode penicillin acylase proteins, but have not been characterized. Initial studies on a pa0305 transposon insertion mutant suggested that the gene is not related to the AHL growth phenotype of P. aeruginosa. The close similarity (67 %) of pa0305 to HacB, an AHL acylase of Pseudomonas syringae, prompted us to investigate whether the PA0305 protein might also function as an AHL acylase. The pa0305 gene has been cloned and the protein (PA0305) has been overproduced, purified and subjected to functional characterization. Analysis of the purified protein showed that, like β-lactam acylases, PA0305 undergoes post-translational processing resulting in α- and β-subunits, with the catalytic serine as the first amino acid of the β-subunit, strongly suggesting that PA0305 is a member of the N-terminal nucleophile hydrolase superfamily. Using a biosensor assay, PA0305his was shown to degrade AHLs with acyl side chains ranging in length from 6 to 14 carbons. Kinetics studies using N-octanoyl-L-homoserine lactone (C(8)-HSL) and N-(3-oxo-dodecanoyl)-L-homoserine lactone (3-oxo-C(12)-HSL) as substrates showed that the enzyme has a robust activity towards these two AHLs, with apparent K(cat)/K(m) values of 0.14 × 10(4) M(-1) s(-1) towards C(8)-HSL and 7.8 × 10(4) M(-1 )s(-1) towards 3-oxo-C(12)-HSL. Overexpression of the pa0305 gene in P. aeruginosa showed significant reductions in both accumulation of 3-oxo-C(12)-HSL and expression of virulence factors. A mutant P. aeruginosa strain with a deleted pa0305 gene showed a slightly increased capacity to kill Caenorhabditis elegans compared with the P. aeruginosa PAO1 wild-type strain and the PAO1 strain carrying a plasmid overexpressing pa0305. The harmful effects of the Δpa0305 strain on the animals were most visible at 5 days post-exposure and the mortality rate of the animals fed on the Δpa0305 strain was faster than for the animals fed on either the wild-type strain or the strain overexpressing pa0305. In conclusion, the pa0305 gene encodes an efficient acylase with activity towards long-chain homoserine lactones, including 3-oxo-C(12)-HSL, the natural quorum sensing signal molecule in P. aeruginosa, and we propose to name this acylase HacB.
authors.affiliation: Faculty of Technobiology, University of Surabaya, Indonesia.|Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Mariana
authors.initials: M
authors.lastname: Wahjudi
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Evelina
authors.initials: E
authors.lastname: Papaioannou
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Oktavia
authors.initials: O
authors.lastname: Hendrawati
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Aart H G
authors.initials: AHG
authors.lastname: van Assen
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Ronald
authors.initials: R
authors.lastname: van Merkerk
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Robbert H
authors.initials: RH
authors.lastname: Cool
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Poelarends
authors.affiliation: Department of Pharmaceutical Biology, University of Groningen, 9713AV Groningen, The Netherlands.
authors.forename: Wim J
authors.initials: WJ
authors.lastname: Quax
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000581:Amidohydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000382:microbiology; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D007783:Lactones / Q000378:metabolism; D010399:Penicillin Amidase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D053038:Quorum Sensing; D017384:Sequence Deletion; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D007783:Lactones; C109860:N-(3-oxododecanoyl)homoserine lactone; C091088:N-octanoylhomoserine lactone; D037521:Virulence Factors; D006714:Homoserine; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; D000581:Amidohydrolases; C438834:N-terminal nucleophile hydrolase; D010399:Penicillin Amidase; C019665:amidase; D015107:4-Butyrolactone