title: Depletion of plasma gelsolin in patients with tick-borne encephalitis and Lyme neuroborreliosis.
abstract: OBJECTIVE
  Cell damage during the course of inflammation results in cytoplasmic actin release, which if not eliminated by the extracellular actin scavenger system, composed of gelsolin and vitamin D binding protein, can cause dysfunction of hemostasis and toxicity towards surrounding cells. In this study, we test the hypothesis that an inflammatory reaction induced by central nervous system infections such as tick-borne encephalitis (TBE) or Lyme neuroborreliosis (LNB) will result in plasma gelsolin concentration changes in the blood and cerebrospinal fluid (CSF).
  
  
  METHODS
  Quantitative Western blot was used to determine gelsolin levels in 58 samples, which include: 29 patients without infection (diagnosed with conditions such as idiopathic cephalalgia, idiopathic Bell's facial nerve palsy and ischialgia due to discopathy in which standard CSF diagnostic tests show no abnormalities), 12 patients diagnosed with TBE, and 17 patients diagnosed with LNB sub forma meningitis.
  
  
  CONCLUSIONS
  The gelsolin concentration in the blood of patients with TBE (163.2 ± 80.8 μg/ml) and LNB (113.6 ± 56.8 μg/ml) was significantly lower (p < 0.05 and p < 0.001, respectively) compared to the control group (226.3 ± 100.7 μg/ml). Furthermore, there was no statistically significant difference between the CSF gelsolin concentration in patients with TBE (3.9 ± 3.3 μg/ml), LNB (2.9 ± 1.2 μg/ml) and the control group (3.7 ± 3.3 μg/ml). An observed decrease in gelsolin concentration in the blood of TBE and LNB patients supports previous findings indicating the involvement of gelsolin in the pathophysiology of an inflammatory response. Therefore, evaluation of blood gelsolin concentration and administration of recombinant plasma gelsolin might provide a new tool to develop diagnostic and therapeutic strategies for TBE and LNB.
authors.affiliation: Department of Neurology, Medical University of Białystok, Białystok, Poland.
authors.forename: Alina
authors.initials: A
authors.lastname: Kułakowska
authors.forename: Joanna M
authors.initials: JM
authors.lastname: Zajkowska
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Ciccarelli
authors.forename: Barbara
authors.initials: B
authors.lastname: Mroczko
authors.forename: Wiesław
authors.initials: W
authors.lastname: Drozdowski
authors.forename: Robert
authors.initials: R
authors.lastname: Bucki
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D015536:Down-Regulation / Q000502:physiology*; D004675:Encephalitis, Tick-Borne / Q000097:blood* / Q000175:diagnosis* / Q000503:physiopathology; D018260:Gelsolin / Q000037:antagonists & inhibitors / Q000097:blood*; D006801:Humans; D020852:Lyme Neuroborreliosis / Q000097:blood* / Q000175:diagnosis* / Q000503:physiopathology; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D018260:Gelsolin