title: Anti-obesity potential of Clerodendron glandulosum.Coleb leaf aqueous extract.
abstract: BACKGROUND
  Clerodendron glandulosum.Coleb leaf aqueous extract (CG) is traditionally used by people of North-East India to alleviate symptoms of diabetes, obesity and hypertension. Previous study from our laboratory have documented anti-diabetic and anti-hypertensive properties of CG extract but, till date there are no pharmacological studies available on its anti-obesity potential. This inventory investigates the underlining molecular mechanism/s of CG induced regulation of in vivo HFD induced obesity and in vitro adipocyte differentiation.
  
  
  OBJECTIVE
  To evaluate effects of CG extract on (i) expression of genes regulating visceral adiposity and (ii) in vitro adipocyte differentiation and LEP release.
  
  
  METHODS
  Body weight, lee index, plasma lipids and LEP, mRNA expression of PPARγ-2, SREBP1c, FAS, CPT-1 and LEP in epididymal adipose tissue of control and experimental groups were evaluated. Also, potential of CG extract on in vitro adipocyte differentiation and LEP release was assessed.
  
  
  RESULTS
  Supplementation of CG extract to HFD fed mice significantly prevented HFD induced increment in bodyweight, lee index, plasma lipids and LEP, visceral adiposity and adipocyte hypertrophy. Also, CG extract supplementation resulted in down regulation of PPARγ-2, SREBP1c, FAS and LEP expression along with up-regulation of CPT-1 in epididymal adipose tissue compared to HFD fed mice. In vitro study recorded significant anti-adipogenic effect of CG extract that resulted in decreased adipogenesis, TG accumulation, LEP release, G3PDH activity along with higher glycerol release without significantly altering viability of 3T3L1 pre-adipocytes.
  
  
  CONCLUSIONS
  Clerodendron glandulosum.Coleb extract prevents adipocyte differentiation and visceral adiposity by down regulation of PPARγ-2 related genes and Lep expression thus validating its traditional therapeutic use in controlling obesity.
authors.affiliation: Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, The M.S. University of Baroda, Gujarat, India.
authors.forename: Ravirajsinh N
authors.initials: RN
authors.lastname: Jadeja
authors.forename: Menaka C
authors.initials: MC
authors.lastname: Thounaojam
authors.forename: Umed V
authors.initials: UV
authors.lastname: Ramani
authors.forename: Ranjitsinh V
authors.initials: RV
authors.lastname: Devkar
authors.forename: A V
authors.initials: AV
authors.lastname: Ramachandran
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D019440:Anti-Obesity Agents / Q000627:therapeutic use*; D001483:Base Sequence; D032409:Clerodendrum / Q000737:chemistry*; D017931:DNA Primers; D004041:Dietary Fats / Q000008:administration & dosage; D005786:Gene Expression Regulation / Q000187:drug effects; D050152:Intra-Abdominal Fat; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014867:Water
chemical_list: D019440:Anti-Obesity Agents; D017931:DNA Primers; D004041:Dietary Fats; D020738:Leptin; D008055:Lipids; D010936:Plant Extracts; D014867:Water