title: Detection of acute brain injury by Raman spectral signature.
abstract: Brain injury can lead to irreversible tissue loss and functional deficit along with significant health care costs. Raman spectroscopy can be used as a non-invasive technique to provide detailed information on the molecular composition of diseased and damaged tissues. This technique was used to examine acute mouse brain injury, focusing on the motor cortex, a region directly involved in controlling execution of movement. The spectral profile obtained from the injured brain tissue revealed a markedly different signature, particularly in the amide I and amide III vibrational region when compared to that of healthy brain tissue. Most noticeably, there was a significant reduction of the amide I vibration at the acute injury site and the appearance of two distinct features at 1586 and 1618 cm(-1). Complementary immunohistochemical analysis of the injured brain tissue showed an abundant expression of Caspase 3 (a cysteine protease marker used for apoptosis), suggesting that the injury-induced specific Raman shifts may be correlated with cell death. Taken together, this study demonstrates that Raman spectroscopy can play an important role in detecting the changes that occur in the injured brain and provide a possible technology for monitoring the recovery process.
authors.affiliation: Institute for Microstructural Sciences, National Research Council Canada, Ottawa, Ontario, Canada K1A 0R6. lilin.tay@nrc.ca
authors.forename: Li-Lin
authors.initials: LL
authors.lastname: Tay
authors.forename: Roger G
authors.initials: RG
authors.lastname: Tremblay
authors.forename: John
authors.initials: J
authors.lastname: Hulse
authors.forename: Bogdan
authors.initials: B
authors.lastname: Zurakowski
authors.forename: Michael
authors.initials: M
authors.lastname: Thompson
authors.forename: Mahmud
authors.initials: M
authors.lastname: Bani-Yaghoub
mesh_terms: D000577:Amides / Q000737:chemistry; D000818:Animals; D001930:Brain Injuries / Q000201:enzymology / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D016002:Discriminant Analysis; D051379:Mice; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000577:Amides; D053148:Caspase 3