title: [An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection].
abstract: A modified RP-HPLC-MS approach has been proposed for a single run separation and identification of the molecular species of different phospholipid classes in a complex extract. This approach has been applied to the analysis of glycero- and sphingolipid composition of human erythrocytes and a number of ceramide fractions have been identified; these fractions was missed in previous studies employing similar methods. The fine experimental design leads to the decrease in the number of procedures needed for a complete phospholipid profiling of the sample.
authors.forename: D A
authors.initials: DA
authors.lastname: Korzhenevskiĭ
authors.forename: A A
authors.initials: AA
authors.lastname: Selishcheva
authors.forename: S V
authors.initials: SV
authors.lastname: Savel'ev
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism
chemical_list: D010743:Phospholipids