title: The use of laminin modified linear ordered collagen scaffolds loaded with laminin-binding ciliary neurotrophic factor for sciatic nerve regeneration in rats.
abstract: Nerve conduit provides a promising strategy for nerve injury repair in the peripheral nervous system (PNS). However, simply bridging the transected nerve with an empty conduit is hard to satisfy functional recovery. The regenerated axons may disperse during regeneration in the empty lumen, limiting the functional recovery. Our previous work had reported that linear ordered collagen scaffold (LOCS) could be used as a nerve guidance material. Here we cross-linked LOCS fibers with laminin which was a major component of the extracellular matrix in nervous system. Ciliary neurotrophic factor (CNTF) plays a critical role in peripheral nerve regeneration. But the lack of efficient CNTF delivery approach limits its clinical applications. To retain CNTF on the scaffold, a laminin binding domain (LBD) was fused to the N-terminal of CNTF. Compared with NAT-CNTF, LBD-CNTF exhibited specific laminin-binding ability and comparable neurotrophic bioactivity. We combined LBD-CNTF with the laminin modified LOCS fibers to construct a double-functional bio-scaffold. The functional scaffold was filled in silicon conduit and tested in the rat sciatic nerve transection model. Results showed that this functional biomaterial could guide the axon growth, retain more CNTF on the scaffolds and enhance the nerve regeneration as well as functional recovery.
authors.affiliation: Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Cao
authors.forename: Changkai
authors.initials: C
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Tiezheng
authors.initials: T
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Jingyu
authors.initials: J
authors.lastname: Wang
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D020934:Ciliary Neurotrophic Factor / Q000737:chemistry* / Q000627:therapeutic use*; D003094:Collagen / Q000737:chemistry*; D004594:Electrophysiology; D007797:Laminin / Q000737:chemistry*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D020934:Ciliary Neurotrophic Factor; D007797:Laminin; D003094:Collagen