title: Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase.
abstract: The effect of overexpression of the trehalose-6-phosphate (T6P) synthase gene (TPS1) on ethanol fermentation of Saccharomyces cerevisiae has been studied at 30 and 38°C. The activity of T6P synthase and the accumulation of trehalose during ethanol fermentation were significantly improved by overexpression of TPS1, and especially at 38°C. Ethanol produced by transformants with and without TPS1 gene overexpression at 38°C was approx. 60 and 37 g/l, respectively. The fermentation efficiency of transformants with TPS1 gene overexpression at 38°C was similar to that at 30°C. The critical growth temperature was increased from 36 to 42°C by TPS1 gene overexpression. These results indicated that overexpression of the TPS1 gene had a beneficial effect on the fermentation capacity of the title yeast strain at high temperatures.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1, Kumamoto, Japan.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: An
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.forename: Kanako
authors.initials: K
authors.lastname: Mitsumasu
authors.forename: Ze-Shen
authors.initials: ZS
authors.lastname: Liu
authors.forename: Morimura
authors.initials: M
authors.lastname: Shigeru
authors.forename: Kida
authors.initials: K
authors.lastname: Kenji
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D013696:Temperature; D014199:Trehalose / Q000378:metabolism
chemical_list: D000431:Ethanol; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase