title: [Effect of neutrophilokines on functional activity of macrophages during formation of immunity against cholera].
abstract: OBJECTIVE
  To study effect of neutrophilokines on functional activity of macrophages (Mph) during formation of immunity against cholera.
  
  
  METHODS
  In order to obtain peritoneal neutrophils (Nph), 2 ml of 0.1% glycogen solution in buffered with phosphates sodium chloride solution was administered intraperitoneally to 100 outbred mice. Vibrio cholerae 1130 in dose 10 microbial cells/Nph and cholera toxin (CT) in dose 1 or 10 mcg/ ml were used as inducers of neutrophilokines synthesis. Obtained neutrophilokines were assessed on their effect on phagocytic activity of Mph, resistance of these cells to cytotoxic and apoptogenic effects of Vibrio cholerae and CT as well as effect on lysosomal apparatus of Mph.
  
  
  RESULTS
  It was established that neutrophilokines induced by Vibrio cholerae and CT stimulate killer activity of Mph and lability of their lysosomal membranes, and suppress programmed death of these cells.
  
  
  CONCLUSIONS
  Results of studies revealed immunoregulatory activity of neutrophilokines relative to Mph and demonstrated ability for cooperation between mono- and polynuclear phagocytes mediated by cytokines and, in particular, neutrophilokines.
authors.forename: G I
authors.initials: GI
authors.lastname: Vasil'eva
authors.forename: I A
authors.initials: IA
authors.lastname: Ivanova
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Tiukavkina
authors.forename: I A
authors.initials: IA
authors.lastname: Bespalova
authors.forename: N D
authors.initials: ND
authors.lastname: Omel'chenko
authors.forename: E P
authors.initials: EP
authors.lastname: Doroshenko
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D002771:Cholera / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis*; D008247:Lysosomes / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D016207:Cytokines; D002772:Cholera Toxin