title: Use of skin equivalent technology in a wound healing model.
abstract: Re-epithelialization is defined as the reconstitution of cells into an organized, stratified squamous epithelium that permanently covers a wound defect and restores function (1). Following wounding, keratinocytes are activated to undergo a series of phenotypic changes that have been well-characterized in vivo (2-4). However, in vitro studies of re-epithelialization have often been limited by their inability to simulate the in vivo tissue. Wound models using skin explants (5-8) or submerged keratinocyte cultures (9,10) demonstrate only partial differentiation and hyperproliferative growth. These systems have been useful for studying keratinoctye migration (11), but are limited in studying other aspects of re-epithelialization.
authors.affiliation: School of Dental Medicine, Department of Oral Biology and Pathology, SUNY at Stony Brook, NY.
authors.forename: M A
authors.initials: MA
authors.lastname: Vaccariello
authors.forename: A
authors.initials: A
authors.lastname: Javaherian
authors.forename: N
authors.initials: N
authors.lastname: Parenteau
authors.forename: J A
authors.initials: JA
authors.lastname: Garlick