title: Analysis of serum protein glycosylation with antibody-lectin microarray for high-throughput biomarker screening.
abstract: The complexity of carbohydrate structures and their derivatives makes the study of the glycome a challenging subset of proteomic research. The microarray platform has become an essential tool to characterize glycan structure and to study glycosylation-related biological interactions, by using probes as a means to interrogate the spotted or captured glycosylated molecules on the arrays. The high-throughput and reproducible nature of microarray platforms have been highlighted by their extensive applications in the field of biomarker validation, where a large number of samples must be analyzed multiple times. This chapter presents an antibody-lectin microarray approach, which allows the efficient, multiplexed study of the glycosylation of multiple individual proteins from complex mixtures with both fluorescence labeling detection and label-free detection based on mass spectrometry.
authors.affiliation: Department of Chemistry, The University of Michigan, Ann Arbor, MI, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: David M
authors.initials: DM
authors.lastname: Lubman
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D006031:Glycosylation; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D011327:Printing; D040081:Protein Array Analysis / Q000379:methods*; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D055910:Antibodies, Immobilized; D015415:Biomarkers; D001798:Blood Proteins; D005456:Fluorescent Dyes; D006023:Glycoproteins; D037102:Lectins