title: Abiotrophia defectiva infection of a total hip arthroplasty diagnosed by 16S rRNA gene sequencing.
abstract: We describe a case of a total hip arthroplasty infection caused by Abiotrophia defectiva, identified by 16S rRNA gene sequencing. Removal of the prosthesis followed by antibiotic treatment resulted in a good clinical outcome. 16S rRNA gene sequencing can be a useful tool in diagnosing infection with this fastidious microorganism that can easily be misidentified using phenotypic identification methods.
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, 1007 MB Amsterdam, The Netherlands. w.rozemeijer@vumc.nl
authors.forename: Wouter
authors.initials: W
authors.lastname: Rozemeijer
authors.forename: Timothy U
authors.initials: TU
authors.lastname: Jiya
authors.forename: Martine
authors.initials: M
authors.lastname: Rijnsburger
authors.forename: Edou
authors.initials: E
authors.lastname: Heddema
authors.forename: Paul
authors.initials: P
authors.lastname: Savelkoul
authors.forename: Wim
authors.initials: W
authors.lastname: Ang
mesh_terms: D058835:Abiotrophia / Q000235:genetics / Q000302:isolation & purification*; D000368:Aged; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery; D006621:Hip Joint / Q000000981:diagnostic imaging / Q000601:surgery; D006622:Hip Prosthesis / Q000009:adverse effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D008853:Microscopy; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology / Q000601:surgery; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011859:Radiography; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S