title: Vapor-liquid-solid grown silica nanowire based electrochemical glucose biosensor.
abstract: Vapor-liquid-solid (VLS) grown silica nanowires (SiO(2)NWs) have been deposited electrophoretically on a gold electrode and utilized for covalent immobilization of glucose oxidase (GOx). Covalent binding has been achieved via 3-aminopropyltriethoxysilane (APTES) modification and N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. Scanning electron microscopy, transmission electron microscopy and cyclic voltammetry techniques have been used to characterize SiO(2)NW and GOx/APTES/SiO(2)NW/Au bioelectrode. Electrochemical studies reveal that SiO(2)NW increases the effective electro-active surface area thus resulting in higher loading of enzyme. Response characteristics show linearity in the range of interest 25-300 mg dl(-1), with a detection limit of 11 mg dl(-1), sensitivity: 0.463 µA (mg dl(-1))(-1) and regression coefficient of 0.992.
authors.affiliation: Bio-MEMS and Microsystem Lab, Department of Electrical Engineering, University of South Florida, Tampa, FL 33620, USA.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Murphy-Pérez
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Arya
authors.forename: Shekhar
authors.initials: S
authors.lastname: Bhansali
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D012822:Silicon Dioxide; D005949:Glucose Oxidase; D005947:Glucose