title: PER-7, an extended-spectrum beta-lactamase with increased activity toward broad-spectrum cephalosporins in Acinetobacter baumannii.
abstract: Acinetobacter baumannii isolate AP2 was recovered from a bronchial lavage sample of a patient hospitalized in Paris, France. A. baumannii AP2 was resistant to all β-lactams, including carbapenems, and expressed the extended-spectrum β-lactamase (ESBL) PER-7, which differs from PER-1 by 4 amino acid substitutions. Compared to PER-1, PER-7 possessed higher-level hydrolytic activities against cephalosporins and aztreonam. The blaPER-7 gene was chromosomally located and associated with a mosaic class 1 integron structure. Additionally, isolate AP2 expressed the carbapenem-hydrolyzing oxacillinase OXA-23 and the 16S RNA methylase ArmA, conferring high-level resistance to aminoglycosides.
authors.affiliation: Service de Bactériologie-Virologie-Hygiène, INSERM U914, Hôpital de Bicêtre, 78 Rue de Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
authors.forename: Rémy A
authors.initials: RA
authors.lastname: Bonnin
authors.forename: Anaïs
authors.initials: A
authors.lastname: Potron
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Hervé
authors.initials: H
authors.lastname: Lecuyer
authors.forename: Rita
authors.initials: R
authors.lastname: Neri
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D002511:Cephalosporins / Q000494:pharmacology*; D002876:Chromosomes, Bacterial / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D001618:beta-Lactamases