title: Preparative separation of atropine and scopolamine from Daturae metelis Flos using pH-zone-refining counter-current chromatography with counter-rotation and dual-mode elution procedure.
abstract: To isolate atropine and scopolamine from Daturae metelis Flos, three different elution modes have been applied in pH-zone-refining counter-current chromatography. These separations were performed with a two-phase solvent system composed of ethyl acetate/n-butanol/water (4:1:5  v/v) with 0.50% triethylamine in the organic phase and 0.15% hydrochloric acid in the aqueous phase. As a result, the best separation was obtained by counter-rotation and dual-mode elution procedure. In this new separation mode, the mobile phase and stationary phase were exchanged when the rotation direction was reversed. The two purified alkaloids (purity over 98% as determined by HPLC) were identified by ESI-MS, (1)H-NMR and (13)C-NMR.
authors.affiliation: Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, PR China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: He
authors.forename: Jianguang
authors.initials: J
authors.lastname: Luo
authors.forename: Lingyi
authors.initials: L
authors.lastname: Kong
mesh_terms: D001285:Atropine / Q000032:analysis / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012601:Scopolamine / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D001285:Atropine; D012601:Scopolamine