title: Overexpression of the c-Myb but not its leukemogenic mutant DNA-binding domain increased adipogenic differentiation in mesenchymal stem cells.
abstract: The c-Myb protein is a vital transcription factor that regulates the differentiation of hematopoietic cells. Previous works have noticed that c-Myb is involved in an epigenetic control mechanism, in which the c-Myb DNA-binding domain (DBD) binds to the N-terminal histone tail of H3 to facilitate it acetylation and activate endogenous differentiation genes, while the leukemogenic mutant of c-Myb does not have these functions. However, whether c-Myb has corresponding biologic functions on the differentiation of other cells except for hematopoietic cells has not been explored. In our studies, we constructed the c-Myb wild type and its leukemogenic mutant DBD recombinant adenovirus with replication-defective adenoviral vectors carrying the GFP gene. We compared their roles on adipogenic differentiation efficiency in human bone marrow-derived mesenchymal stem cells (hMSCs). Our results demonstrated that the overexpression of c-Myb could enhance adipogenic differentiation in hMSCs, while the overexpression of its leukemogenic mutant blocked the adipogenic differentiation to a certain extent. These suggest that c-Myb play an important role in the hMSCs differentiation too, which is consistent with the epigenetic control mechanism of c-Myb.
authors.affiliation: Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yilan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Luo
authors.forename: Chongjie
authors.initials: C
authors.lastname: Zhang
mesh_terms: D050156:Adipogenesis; D000293:Adolescent; D002478:Cells, Cultured; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009154:Mutation; D017434:Protein Structure, Tertiary / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D020598:Proto-Oncogene Proteins c-myb