title: Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability.
abstract: We evaluated the possible anticancer performance of a natural compound, goniothalamin (GTN), against human lung cancer using as a non-small cell lung cancer (NSCLC) cell line, H1299, as the model system. Cellular proliferation was significantly inhibited by GTN. Using an improved alkaline comet-nuclear extract (comet-NE) assay, GTN was found to induce a significant increase in the tail DNA. Wound healing and zymography assays showed that GTN attenuated cell migration and caused a reduction in the activity level of two major migration-associated matrix metalloproteinases, MMP-2 and MMP-9. It can be concluded that the DNA-damaging effect of GTN against lung cancer cells leads to growth inhibition as well as a depression in migration ability. Therefore, GTN has potential as a chemotherapeutic agent against lung cancer.
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.forename: Po-Len
authors.initials: PL
authors.lastname: Liu
authors.forename: Kuang-Jing
authors.initials: KJ
authors.lastname: Huang
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Chang
authors.forename: Chon-Kit
authors.initials: CK
authors.lastname: Chou
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.forename: Inn-Wen
authors.initials: IW
authors.lastname: Chong
authors.forename: Kang
authors.initials: K
authors.lastname: Fang
authors.forename: Jia-Shing
authors.initials: JS
authors.lastname: Chen
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D011753:Pyrones / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011753:Pyrones; C103280:goniothalamin