title: DC-SIGN mediated antigen-targeting using glycan-modified liposomes: formulation considerations.
abstract: Dendritic cells (DCs) are key antigen presenting cells that have the unique ability to present antigens on MHC molecules, which can lead to either priming or suppression of T cell mediated immune responses. C-type lectin receptors expressed by DCs are involved in antigen uptake and presentation through recognition of carbohydrate structures on antigens. Here we have explored the feasibility of modification of liposomes with glycans for targeting purposes to boost immune responses. The potential of targeting glycoliposomal constructs to the C-type lectin DC-SIGN on DCs was studied using either PEGylated or non-PEGylated liposomes. Our data demonstrate that formulation of the glycoliposomes as PEGylated negatively affected their potential to target to DCs.
authors.affiliation: Department of Pharmaceutics, Utrecht University of Pharmaceutical Sciences, Utrecht University, The Netherlands.
authors.forename: Medha D
authors.initials: MD
authors.lastname: Joshi
authors.forename: Wendy W J
authors.initials: WW
authors.lastname: Unger
authors.forename: Astrid J
authors.initials: AJ
authors.lastname: van Beelen
authors.forename: Sven C
authors.initials: SC
authors.lastname: Bruijns
authors.forename: Manja
authors.initials: M
authors.lastname: Litjens
authors.forename: Louis
authors.initials: L
authors.lastname: van Bloois
authors.forename: Hakan
authors.initials: H
authors.lastname: Kalay
authors.forename: Yvette
authors.initials: Y
authors.lastname: van Kooyk
authors.forename: Gert
authors.initials: G
authors.lastname: Storm
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology; D016503:Drug Delivery Systems; D005240:Feasibility Studies; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism*; D008081:Liposomes; D008285:Major Histocompatibility Complex / Q000276:immunology*; D011092:Polyethylene Glycols / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D008081:Liposomes; D011092:Polyethylene Glycols; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin