title: In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production.
abstract: A simple and practicable method for in-gel detection of bacterial produced L-amino acid oxidases (L-AAOs) after non-denaturing SDS-PAGE based on the visualisation of occurring hydrogen peroxide production is described. Advantages of this screening method for L-AAOs are the determination of their numbers and approximate molecular weights additionally in one approach.
authors.affiliation: Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobener Strasse, D-28359 Bremen, Germany. jerau@uni-bremen.de
authors.forename: Jan Erik
authors.initials: JE
authors.lastname: Rau
authors.forename: Ulrich
authors.initials: U
authors.lastname: Fischer
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D050830:L-Amino Acid Oxidase / Q000032:analysis* / Q000302:isolation & purification; D010084:Oxidation-Reduction
chemical_list: D000596:Amino Acids; D006861:Hydrogen Peroxide; D050830:L-Amino Acid Oxidase