title: Migration and ramification of microglia in quail embryo retina organotypic cultures.
abstract: Organotypic cultures of retina explants preserve the complex cellular microenvironment of the retina and have been used as a tool to assess the biological functions of some cell types. However, studies to date have shown that microglial cells activate quickly in response to the retina explantation. In this study, microglial cells migrated and ramified in quail embryo retina organotypic cultures (QEROCs) according to chronological patterns bearing a resemblance to those in the retina in situ, despite some differences in cell density and ramification degree. Retinal explants from quail embryos at 9 days of incubation (E9) proved to be the best in vitro system for reproducing a physiological-like behavior of microglial cells when cultured in Eagle's basal medium supplemented with horse serum. During the first week in vitro, microglial cells migrated tangentially in the vitreal part of QEROCs, and some began to migrate radially from 3 days in vitro (div) onward, ramifying in the inner and outer plexiform layers, thus mimicking microglia development in the retina in situ, although reaching a lower degree of ramification after 7 div. From 8 div onward, microglial cells rounded throughout the explant thickness simultaneously with the nonphysiological appearance of dead photoreceptors and round microglia in the outernuclear layer. Therefore, E9 QEROCs can be used during the first week in vitro as a model system for experimental studies of molecules putatively involved in microglial migration and ramification.
authors.affiliation: Departamento de Biología Celular, Facultad de Ciencias, Universidad de Granada, E-18071 Granada, Spain.
authors.forename: Maria-Carmen
authors.initials: MC
authors.lastname: Carrasco
authors.forename: Julio
authors.initials: J
authors.lastname: Navascués
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Cuadros
authors.forename: Ruth
authors.initials: R
authors.lastname: Calvente
authors.forename: David
authors.initials: D
authors.lastname: Martín-Oliva
authors.forename: Ana M
authors.initials: AM
authors.lastname: Santos
authors.forename: Ana
authors.initials: A
authors.lastname: Sierra
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Ferrer-Martín
authors.forename: José L
authors.initials: JL
authors.lastname: Marín-Teva
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D003370:Coturnix; D017628:Microglia / Q000166:cytology / Q000502:physiology*; D009924:Organ Culture Techniques; D010786:Photoreceptor Cells / Q000166:cytology / Q000502:physiology; D012160:Retina / Q000166:cytology* / Q000196:embryology*