title: Antitumour compounds from a saline soil isolate, Streptomyces griseoincarnatus CTF15.
abstract: A new actinomycete strain designated as Streptomyces sp. CTF15 was isolated from a saline soil using casein-KNO(3) agar medium. The strain Streptomyces sp. CTF15 exhibited promising antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Streptomyces viridochromogens Tu57 and high cytotoxicity (91.2% mortality) against Artimia salina in biological screening. The cultivation of this strain in a 50 L lab fermenter and subsequent isolation and purification by a series of chromatographic techniques and structure elucidation by MS and NMR analysis of the active metabolites revealed that it is a highly stable producer of resistomycin (1), tetracenomycin D (2) and actinomycin D (3), even under non-optimised culture conditions. The morphological, microscopic, biochemical and physiological characterisation suggested that the strain CTF15 belongs to the genus Streptomyces. A partial 16S rRNA gene sequence (1429 bp) from the strain CTF15 was determined and found to have high identity (99%) with Streptomyces griseoincarnatus. As such, this is the first report of a strain of S. griseoincarnatus capable of producing these three bioactive compounds simultaneously.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
authors.forename: Imran
authors.initials: I
authors.lastname: Sajid
authors.forename: Khaled A
authors.initials: KA
authors.lastname: Shaaban
authors.forename: Shahida
authors.initials: S
authors.lastname: Hasnain
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis / Q000302:isolation & purification*; D000970:Antineoplastic Agents / Q000032:analysis / Q000302:isolation & purification*; D001580:Benzopyrenes / Q000032:analysis; D003609:Dactinomycin / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009279:Naphthacenes / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D001580:Benzopyrenes; D009279:Naphthacenes; D012336:RNA, Ribosomal, 16S; C057303:tetracenomycin D3; D003609:Dactinomycin; C018379:resistomycin