title: Automated applications of sandwich-cultured hepatocytes in the evaluation of hepatic drug transport.
abstract: Predictions of the absorption, distribution, metabolism, excretion, and toxicity of compounds in pharmaceutical development are essential aspects of the drug discovery process. B-CLEAR is an in vitro system that uses sandwich-cultured hepatocytes to evaluate and predict in vivo hepatobiliary disposition (hepatic uptake, biliary excretion, and biliary clearance), transporter-based hepatic drug-drug interactions, and potential drug-induced hepatotoxicity. Automation of predictive technologies is an advantageous and preferred format in drug discovery. In this study, manual and automated studies are investigated and equivalence is demonstrated. In addition, automated applications using model probe substrates and inhibitors to assess the cholestatic potential of drugs and evaluate hepatic drug transport are examined. The successful automation of this technology provides a more reproducible and less labor-intensive approach, reducing potential operator error in complex studies and facilitating technology transfer.
authors.affiliation: Qualyst, Inc., Durham, NC 27713, USA. cassandraperry@qualyst.com
authors.forename: Cassandra H
authors.initials: CH
authors.lastname: Perry
authors.forename: William R
authors.initials: WR
authors.lastname: Smith
authors.forename: Robert L
authors.initials: RL
authors.lastname: St Claire
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Brouwer
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D001692:Biological Transport / Q000187:drug effects; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004341:Drug Evaluation / Q000379:methods*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: D000924:Anticholesteremic Agents; D001647:Bile Acids and Salts; D026901:Membrane Transport Proteins