title: Anticolchicine cytotoxicity enhanced by Dan Gua-Fang, a Chinese herb prescription in ECV304 in mediums.
abstract: OBJECTIVE
  To study the effect of anticolchicine cytotoxicity of Dan Gua-Fang, a Chinesea Chinese), a Chinese herbal compound prescription on endothelial cells of vein (ECV304) cultivated in mediums of different glucose concentrations as well as the proliferation of those cells in the same conditions, in order to reveal the value of Dan Gua-Fang in preventing and treating endothelial damage caused by hyperglycemia in diabetes mellitus.
  
  
  METHODS
  The research was designed as three stages. The growing state and morphological changes were observed when ECV304 were cultivated in the culture mediums, which have different glucose concentrations with or without Dan Gua-Fang and at the same time with or without colchicine.
  
  
  RESULTS
  (1) Dan Gua-Fang at all concentrations reduced the floating cell population of ECV304 cultivated in hyperglycemia mediums. (2) Dan Gua-Fang at all concentrations and hyperglycemia both had a function of promoting "pseudopod-like" structure formation in cultivated ECV304, but the function was not superimposed in mediums containing both hyperglycemia and Dan Gua-Fang. (3) Colchicine reduced and even vanished the "pseudopod-like" structure of the endotheliocyte apparently cultivated in mediums of hyperglycemia or with Dan Gua-Fang. The "pseudopod-like" structure of the endotheliocyte emerged quickly in Dan Gua-Fang groups after colchicine was removed, but it was not the case in hyperglycemia only without Dan Gua-Fang groups. (4) Dan Gua-Fang reduced the mortality of cells cultivated in mediums containing colchicine. The cell revived to its normal state fast after colchicine was removed.
  
  
  CONCLUSIONS
  Dan Gua-Fang has the functions of promoting the formation of cytoskeleton and fighting against colchicine cytotoxicity.
authors.affiliation: Department of Endocrinology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China. hengxianpei@hotmail.com
authors.forename: Xian-Pei
authors.initials: XP
authors.lastname: Heng
authors.forename: Ke-Ji
authors.initials: KJ
authors.lastname: Chen
authors.forename: Zhen-Feng
authors.initials: ZF
authors.lastname: Hong
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: He
authors.forename: Ke-Dan
authors.initials: KD
authors.lastname: Chu
authors.forename: Wen-Lie
authors.initials: WL
authors.lastname: Chen
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Zheng
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D003078:Colchicine / Q000009:adverse effects* / Q000037:antagonists & inhibitors*; D003470:Culture Media / Q000009:adverse effects / Q000494:pharmacology; D019610:Cytoprotection / Q000187:drug effects; D003603:Cytotoxins / Q000009:adverse effects / Q000037:antagonists & inhibitors; D004336:Drug Antagonism; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000009:adverse effects / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D003603:Cytotoxins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C559220:dan gua-fang; D005947:Glucose; D003078:Colchicine