title: [Amanitin determination in mushroom poisoning diagnostics].
abstract: There are some serious poisonings with toxic mushroom species in Poland every year. Good prognostics in the cases is correlated to short time from mushroom consumption to hospitalization, correct distinguish not specific gastrointestinal and Amanita phalloides syndrome and immediately specific treatment. The purpose of the paper was to make appraisal of usefulness of amanitin blood and urine determination and transaminases activity determination (ALT, AST) in diagnostics of mushroom poisoned patients up to three days after mushroom consumption. The material was twenty two retrospective histories of mushroom poisoned patients treated in the years 2007-2008. Amanitin blood and urine determinations were made by ELISA method. Urine amanitin results in samples collected within 40 h from mushroom consuming were positive in all Amanita phalloides syndrome cases. Serum amanitin determination was not useful for the diagnostics. Trans-aminases activity determinations let to distinguish Amanita phalloides syndrome on the second and the third day after mushroom consumption. In the first poisoning phase (within 24 h), the ALT and AST activities were in normal ranges and only amanitin urine determination let to confirm or exclude Amanita phalloides poisoning. Amanitin urine determinations were useful to take fast decision about specific treatment and avoid internal organs dysfunctions.
authors.affiliation: Pracownia Toksykologii Analitycznej i Terapii Monitorowanej, Katedra Toksykologii i Chorób, Srodowiskowych, UJCM w Krakowie. egomolka@cm-uj.krakow.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Gomółka
authors.forename: Dorota
authors.initials: D
authors.lastname: Szpak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Morawska
authors.forename: Jolanta
authors.initials: J
authors.lastname: Wilimowska
mesh_terms: D000546:Amanitins / Q000097:blood* / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D009145:Mushroom Poisoning / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D011044:Poland
chemical_list: D000546:Amanitins