title: Morphological development and expression of neurotrophin receptors in the laryngeal sensory corpuscles.
abstract: Morphological development of sensory structures in the laryngeal mucosa of postnatal rats was observed by use of immunohistochemistry for protein gene-product 9.5 (PGP9.5). Moreover, expression changes of high affinity neurotrophin receptors, TrkA, TrkB and TrkC, and low affinity neurotrophin receptor p75(NTR) were examined to elucidate the relationship to morphogenesis. Intraepithelial nerve endings and parent axons of the laminar endings with immunoreactivity for PGP9.5 have already appeared in the rat on embryonic day 18 (E18) as well as solitary chemoreceptor cells in the glottic cleft. According to neurotrophin receptors, TrkA immunoreactivity were observed on and after postnatal week 3 (3W) in the nervous sensory structures, that is, free nerve endings, laminar endings and sub- and intragemmal plexuses of the taste buds. In the laminar endings, TrkC immunoreactivity was also observed on and after 3W. According to the laryngeal sensory cells, the solitary chemoreceptor cells were immunoreactive to TrkA, TrkB, and TrkC on and after postnatal day 3 (P3). In the taste buds in arytenoid region, taste cells were immunoreactive for TrkA, TrkB, and TrkC on and after 3W, P14, and 3W, respectively. Immunoreactivity for p75(NTR) was observed on the surface of taste cells on and after P9. The results of the present study suggest that sensory structures in the laryngeal mucosa were developed on perinatal days to involve respiratory reflex, and that neurotrophin receptors may take part in the regulation and maintenance of sensory structures.
authors.affiliation: Laboratory of Veterinary Biochemistry and Cell Biology, Faculty of Agriculture, Department of Veterinary Sciences, Iwate University, Morioka, Iwate, Japan. yyoshio@iwate-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Momo
authors.initials: M
authors.lastname: Hashiguchi
authors.forename: Misuzu
authors.initials: M
authors.lastname: Yamaguchi-Yamada
mesh_terms: D000818:Animals; D002628:Chemoreceptor Cells / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D007150:Immunohistochemistry; D007820:Laryngeal Mucosa / Q000196:embryology / Q000254:growth & development / Q000294:innervation*; D009024:Morphogenesis; D009419:Nerve Tissue Proteins; D051381:Rats; D017208:Rats, Wistar; D020917:Receptor, trkA / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism; D020812:Receptor, trkC / Q000378:metabolism; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000378:metabolism*; D013650:Taste Buds / Q000378:metabolism; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; C068313:Ngfr protein, rat; D020917:Receptor, trkA; D020813:Receptor, trkB; D020812:Receptor, trkC; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase