title: Characterization of acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) enzyme of human small intestine.
abstract: Acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) enzyme plays a significant role in dietary triacylglycerol (TAG) absorption in the small intestine. However, the characteristics of human intestinal DGAT enzyme have not been examined in detail. The aim of our study was to characterize the human intestinal DGAT enzyme by examining acyl-CoA specificity, temperature dependency, and selectivity for 1,2-diacylglycerol (DAG) or 1,3-DAG. We detected DGAT activity of human intestinal microsome and found that the acyl-CoA specificity and temperature dependency of intestinal DGAT coincided with those of recombinant human DGAT1. To elucidate the selectivity of human intestinal DGAT to 1,2-DAG or 1,3-DAG, we conducted acyl-coenzyme A:monoacylglycerol acyltransferase assays using 1- or 2-monoacylglycerol (MAG) as substrates. When 2-MAG was used as acyl acceptor, both 1,2-DAG and TAG were generated; however, when 1-MAG was used, 1,3-DAG was predominantly observed and little TAG was detected. These findings suggest that human small intestinal DGAT, which is mainly encoded by DGAT1, utilizes 1,2-DAG as the substrate to form TAG. This study will contribute to understand the lipid absorption profile in the small intestine.
authors.affiliation: Dainippon-Sumitomo Pharma Co., Ltd., 3-1-98 Kasugade-naka, Konohana-ku, 554-0022 Osaka, Japan. yasushi-hiramine@ds-pharma.co.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiramine
authors.forename: Toshizumi
authors.initials: T
authors.lastname: Tanabe
mesh_terms: D000214:Acyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology* / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D013379:Substrate Specificity; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000214:Acyl Coenzyme A; D014280:Triglycerides; C494881:DGAT1 protein, human; D051048:Diacylglycerol O-Acyltransferase