title: Milky hemolymph syndrome (MHS) in spiny lobsters, penaeid shrimp and crabs.
abstract: Black tiger shrimp Penaeus monodon, European shore crab Carcinus maenas and spiny lobster Panulirus spp. can be affected by milky hemolymph syndrome (MHS). Four rickettsia-like bacteria (RLB) isolates of MHS originating from 5 geographical areas have been identified to date. The histopathology of the disease was characterized and a multiplex PCR assay was developed for detection of the 4 bacterial isolates. The 16S rRNA gene and 16-23S rRNA intergenic spacer region (ISR) were used to examine the phylogeny of the MHS isolates. Although the pathology of this disease appears similar in the various different hosts, sequencing and examination of the phylogenetic relationships reveal 4 distinct RLB involved in the infection process.
authors.affiliation: Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA. lmn@email.arizona.edu
authors.forename: Linda M
authors.initials: LM
authors.lastname: Nunan
authors.forename: Bonnie T
authors.initials: BT
authors.lastname: Poulos
authors.forename: Solangel
authors.initials: S
authors.lastname: Navarro
authors.forename: Rita M
authors.initials: RM
authors.lastname: Redman
authors.forename: Donald V
authors.initials: DV
authors.lastname: Lightner
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D003386:Brachyura / Q000382:microbiology*; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006458:Hemolymph / Q000382:microbiology*; D033521:Palinuridae / Q000382:microbiology*; D033561:Penaeidae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S