title: Enhancement of gastrointestinal absorption of ovalbumin caused by spermine induces an increase in plasma histamine levels in mice sensitized to ovalbumin.
abstract: The aim of this study was to determine whether a spermine (SPM)-induced increase in gastrointestinal absorption of an allergen leads to an anaphylactic response in sensitized mice. First, we examined the enhancing effect of SPM on the gastrointestinal absorption of ovalbumin (OVA) in an in situ jejunum loop study in rats and an in vivo oral absorption study in mice. Second, we investigated whether enhancement of gastrointestinal absorption of OVA caused by SPM induces an anaphylactic response in mice sensitized to OVA. In the in situ jejunum loop study in rats, a significant amount of immune-reactive OVA was detected in the plasma after co-administration of OVA and SPM. Oral co-administration of OVA and SPM to mice in vivo also increased plasma OVA concentrations in an SPM dose-dependent manner. Furthermore, in sensitized mice, a significant increase in plasma histamine levels occurred along with the increase in plasma OVA levels after co-administration of OVA with SPM. This finding suggests that an SPM-induced increase in gastrointestinal absorption of OVA leads to an anaphylactic response. These results indicate that excess oral ingestion of SPM may have widespread health effects, including the induction of food allergies, via modulation of the function of the gastrointestinal epithelial barrier.
authors.affiliation: Laboratory of Cellular Physiology, Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350–0295, Japan. sugita@josai.ac.jp
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Sugita
authors.forename: Koichi
authors.initials: K
authors.lastname: Takao
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sugino
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kuwabara
authors.forename: Akira
authors.initials: A
authors.lastname: Shirahata
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced*; D000818:Animals; D004357:Drug Synergism; D005260:Female; D006632:Histamine / Q000097:blood*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism*; D007583:Jejunum / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000009:adverse effects / Q000276:immunology / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000009:adverse effects*
chemical_list: D013096:Spermine; D006632:Histamine; D010047:Ovalbumin