title: An automatic optosensing device for the simultaneous determination of resveratrol and piceid in wines.
abstract: For the first time, a spectrofluorimetric method is reported for the simultaneous determination of resveratrol (RVT) and piceid (PCD), two stilbenes showing diverse interesting physiological and biochemical attributes, as well as a wide range of health benefits ranging from cardioprotection to chemoprevention. The method makes use of a multicommutated flow-through optosensor in which the resolution of RVT and PCD is accomplished by means the sequential arrival of their photoproducts, on-line generated by UV-irradiation, to the detection area. This is possible due to the different kinetic behaviour of these latter on a solid support (C(18) silica gel) filling a minicolumn placed before the detector. The measurement in solid-phase of the photochemically induced fluorescence of the photoproducts (λ(ex): 257 nm/λ(em): 382 nm) is used as analytical signal for monitoring both compounds. The method has been applied to the analysis of RVT and PCD in wines and requires a previous solid-phase extraction (SPE) using Bakerbond C(18) cartridges. This pretreatment and the use of a solid-support in both the minicolumn and the flow-cell of the detector allow the determination of RVT and PCD by external calibration. Detection limits (DLs) are 9.3 and 12.6 ng mL(-1) for RVT and PCD, respectively. Commercial red and white wine samples have been analysed and the results obtained have been satisfactorily validated by high-performance liquid chromatography (HPLC).
authors.affiliation: Department of Physical and Analytical Chemistry, University of Jaén, Spain.
authors.forename: Lucía
authors.initials: L
authors.lastname: Molina-García
authors.forename: Antonio
authors.initials: A
authors.lastname: Ruiz-Medina
authors.forename: María Luisa Fernández-de
authors.initials: ML
authors.lastname: Córdova
mesh_terms: D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D000077185:Resveratrol; D052616:Solid Phase Extraction / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013267:Stilbenes / Q000032:analysis* / Q000302:isolation & purification; D014920:Wine / Q000032:analysis*
chemical_list: D005960:Glucosides; D013267:Stilbenes; D000077185:Resveratrol; C058229:polydatin