title: Identification of a glycosyltransferase from Mycobacterium marinum involved in addition of a caryophyllose moiety in lipooligosaccharides.
abstract: Deletion of Mycobacterium marinum MMAR2333 resulted in the loss of three of four subclasses of lipooligosaccharides (LOSs). The mutant was unable to extend an intermediate (LOS-II*) by addition of caryophyllose. These data and the predicted domain structure suggest that MMAR2333 is a glycosyltransferase involved in the generation of a lipid-linked caryophyllose donor.
authors.affiliation: School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
authors.forename: Debasmita
authors.initials: D
authors.lastname: Sarkar
authors.forename: Mandeep
authors.initials: M
authors.lastname: Sidhu
authors.forename: Albel
authors.initials: A
authors.lastname: Singh
authors.forename: Jiemin
authors.initials: J
authors.lastname: Chen
authors.forename: David A
authors.initials: DA
authors.lastname: Lammas
authors.forename: Astrid M
authors.initials: AM
authors.lastname: van der Sar
authors.forename: Gurdyal S
authors.initials: GS
authors.lastname: Besra
authors.forename: Apoorva
authors.initials: A
authors.lastname: Bhatt
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002241:Carbohydrates; D002473:Cell Wall / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D019910:Mycobacterium marinum / Q000201:enzymology* / Q000235:genetics; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D002241:Carbohydrates; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D016695:Glycosyltransferases