title: A simple HPLC method for quantitation of quercetin in herbal extracts.
abstract: A reversed-phase HPLC method with UV detection was developed for the determination of quercetin. The method produced linear response over a wide concentration range, with an average accuracy of 95% and average intra- and interday variation of 0.75 and 0.3, respectively. The exactness of the method was proven by determining the recovery rates from 50 to 150% of standard concentration, which were found within the acceptable range of 95 to 105%. The method was used for quantitation of quercetin in the extracts of Psidium guajava, Vitis vinifera, and extracts rich in quercetin and other flavonols in the flavonoid family.
authors.affiliation: Principal K.M. Kundnani College of Pharmacy, Jote Joy Bldg, Rambhau Salgaokar Marg, Cuffe Parade, Colaba, Mumbai 400 005, Maharashtra, India. dmellopm@rediffmail.com
authors.forename: Priscilla M
authors.initials: PM
authors.lastname: D'Mello
authors.forename: Urmila J
authors.initials: UJ
authors.lastname: Joshi
authors.forename: Preetam P
authors.initials: PP
authors.lastname: Shetgiri
authors.forename: Tarun K
authors.initials: TK
authors.lastname: Dasgupta
authors.forename: Kamil K
authors.initials: KK
authors.lastname: Darji
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis / Q000592:standards; D044948:Flavonols / Q000032:analysis; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D031647:Psidium / Q000737:chemistry; D011794:Quercetin / Q000032:analysis* / Q000592:standards; D012015:Reference Standards; D027843:Vitis / Q000737:chemistry
chemical_list: D044948:Flavonols; D010936:Plant Extracts; D011794:Quercetin