title: Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong.
abstract: OBJECTIVE
  We characterized plasmids encoding CTX-M-14 β-lactamase originating from Escherichia coli isolates recovered from patients with uncomplicated cystitis or individuals with faecal colonization in Hong Kong from 2002 to 2004.
  
  
  METHODS
  Plasmids carrying CTX-M-14 were studied by conjugation, replicon typing, S1 nuclease-PFGE and plasmid PCR-restriction fragment length polymorphism (RFLP). The complete sequence of pHK01, a 70 kb plasmid encoding CTX-M-14 from an E. coli strain, was determined and the results compared with reference plasmids and aligned with GenBank data.
  
  
  RESULTS
  The blaCTX-M-14 plasmids could be transferred in 23 of 44 E. coli strains tested. Among the 23 transconjugants, the replicon types of the CTX-M-14-encoding plasmid were FII (n=13), I1-Iγ (n=4), F1B (n=2), FII and I1-Iγ (n=1), K (80 kb, n=1) and undetermined (n=2). Plasmid pHK01 (FII replicon) shares a high degree of homology with R100 except mainly for a 11 kb variable region containing blaCTX-M-14 (with an upstream ISEcp1 and a downstream truncated IS903), an iron transport system, an outer membrane protein (malB, maltoporin) and a putative toxin-antitoxin plasmid stability system (yacABC). It was highly related to blaCTX-M-14 (pKF3-70) and blaCTX-M-24 (pEG356) plasmids reported from mainland China in 2006 and Vietnam in 2007, respectively. Subtyping by a plasmid PCR-RFLP scheme showed that 10 of the 13 FII plasmids originating from isolates collected by multiple laboratories exhibited either identical or highly similar profiles.
  
  
  CONCLUSIONS
  This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community.
authors.affiliation: Department of Microbiology, University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong, People's Republic of China. plho@hkucc.hku.hk
authors.forename: P L
authors.initials: PL
authors.lastname: Ho
authors.forename: W U
authors.initials: WU
authors.lastname: Lo
authors.forename: River C W
authors.initials: RC
authors.lastname: Wong
authors.forename: M K
authors.initials: MK
authors.lastname: Yeung
authors.forename: K H
authors.initials: KH
authors.lastname: Chow
authors.forename: T L
authors.initials: TL
authors.lastname: Que
authors.forename: Amy H Y
authors.initials: AH
authors.lastname: Tong
authors.forename: Jessie Y J
authors.initials: JY
authors.lastname: Bao
authors.forename: Si
authors.initials: S
authors.lastname: Lok
authors.forename: Samson S Y
authors.initials: SS
authors.lastname: Wong
mesh_terms: D000328:Adult; D002648:Child; D002675:Child, Preschool; D003227:Conjugation, Genetic; D003556:Cystitis / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D005838:Genotype; D006723:Hong Kong; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; C456080:beta-lactamase CTX-M-14; D001618:beta-Lactamases