title: CDK11p46 and RPS8 associate with each other and suppress translation in a synergistic manner.
abstract: CDK11p46, a 46kDa isoform of the PITSLRE kinase family, is a key mediator of cell apoptosis, while the precise mechanism remains to be elucidated. By using His pull-down and mass spectrometry analysis, we identified the ribosomal protein S8 (RPS8), a member of the small subunit ribosome, as an interacting partner of CDK11p46. Further analysis confirmed the association of CDK11p46 and RPS8 in vitro and in vivo, and revealed that RPS8 was not a substrate of CDK11p46. Moreover, RPS8 and CDK11p46 synergize to inhibit the translation process both in cap- and internal ribosomal entry site (IRES)-dependent way, and sensitize cells to Fas ligand-induced apoptosis. Taken together, our results provide evidence for the novel role of CDK11p46 in the regulation of translation and cell apoptosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Shanghai, Medical College, Fudan University, Shanghai, PR China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Hao
authors.forename: Xiangfei
authors.initials: X
authors.lastname: Kong
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ruan
authors.forename: Huachen
authors.initials: H
authors.lastname: Gan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Chunyi
authors.initials: C
authors.lastname: Zhang
authors.forename: Shifang
authors.initials: S
authors.lastname: Ren
authors.forename: Jianxin
authors.initials: J
authors.lastname: Gu
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000494:pharmacology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D012269:Ribosomal Proteins; C030254:ribosomal protein S8; C447612:CDK11a protein, human; D018844:Cyclin-Dependent Kinases