title: SPOT synthesis as a tool to study protein-protein interactions.
abstract: Peptide arrays are a widely used tool in proteomic research for investigations of drug development and molecular interactions including protein-protein or protein-peptide interactions. Most peptide synthesis techniques are able to simultaneously synthesize only up to a few hundred single peptides. Using the SPOT™ technique, it is possible to synthesize and screen in parallel up to 8,000 peptides or peptide mixtures. In addition, such peptides can be released from the membrane and transferred onto peptide microarrays. Here we present protocols for the peptides synthesis on cellulose including the preparation of different cellulose membranes and easy-to-use detection methods on these peptide macroarrays. In addition, a protocol to produce and screen peptide microarray using the SPOT technology is provided.
authors.affiliation: Peptide Facility, Kinexus Bioinformatics Corporation, Vancouver, BC, Canada.
authors.forename: Dirk F H
authors.initials: DF
authors.lastname: Winkler
authors.forename: Heiko
authors.initials: H
authors.lastname: Andresen
authors.forename: Kai
authors.initials: K
authors.lastname: Hilpert
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000577:Amides / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004951:Esterification; D004987:Ethers / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D008163:Luminescent Measurements; D008567:Membranes, Artificial; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D013050:Spectrometry, Fluorescence
chemical_list: D000577:Amides; D004987:Ethers; D008567:Membranes, Artificial; D010455:Peptides; D002482:Cellulose; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase