title: Modification of the BAX System PCR assay for detecting Salmonella in beef, produce, and soy protein isolate. Performance Tested Method 100201.
abstract: The BAX System PCR assay for Salmonella detection in foods was previously validated as AOAC Research Institute (RI) Performance Tested Method (PTM) 100201. New studies were conducted on beef and produce using the same media and protocol currently approved for the BAX System PCR assay for E. coli O157:H7 multiplex (MP). Additionally, soy protein isolate was tested for matrix extension using the U.S. Food and Drug Administration-Bacteriological Analytical Manual (FDA-BAM) enrichment protocols. The studies compared the BAX System method to the U.S. Department of Agriculture culture method for detecting Salmonella in beef and the FDA-BAM culture method for detecting Salmonella in produce and soy protein isolate. Method comparison studies on low-level inoculates showed that the BAX System assay for Salmonella performed as well as or better than the reference method for detecting Salmonella in beef and produce in 8-24 h enrichment when the BAX System E. coli O157:H7 MP media was used, and soy protein isolate in 20 h enrichment with lactose broth followed by 3 h regrowth in brain heart infusion broth. An inclusivity panel of 104 Salmonella strains with diverse serotypes was tested by the BAX System using the proprietary BAX System media and returned all positive results. Ruggedness factors involved in the enrichment phase were also evaluated by testing outside the specified parameters, and none of the factors examined affected the performance of the assay.
authors.affiliation: DuPont Qualicon, Rt. 141 & Henry Clay Rd, Wilmington, DE 19880-0400, USA.
authors.forename: Linda X
authors.initials: LX
authors.lastname: Peng
authors.forename: Morgan
authors.initials: M
authors.lastname: Wallace
authors.forename: Bridget
authors.initials: B
authors.lastname: Andaloro
authors.forename: Dawn
authors.initials: D
authors.lastname: Fallon
authors.forename: Lois
authors.initials: L
authors.lastname: Fleck
authors.forename: Dan
authors.initials: D
authors.lastname: Delduco
authors.forename: George
authors.initials: G
authors.lastname: Tice
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D002417:Cattle; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005516:Food Microbiology / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D030262:Soybean Proteins; D014481:United States; D014486:United States Food and Drug Administration; D014675:Vegetables / Q000382:microbiology
chemical_list: D030262:Soybean Proteins