title: Transgenic mice for cre-inducible overexpression of the Cul4A gene.
abstract: The Cullin 4A(Cul4A) gene is important in cell survival, development, growth, and cell cycle control and is amplified in breast and hepatocellular cancers. Recently, we reported that Cul4A plays an oncogenic role in the pathogenesis of mesothelioma. An important strategy for studying Cul4A in different tissues is targeted overexpression of this gene in vivo. Studies of Cul4A in mice have been restricted to the loss-of-function studies using Cul4A knockout mice; gain-of-function studies of Cul4A using transgenic mice have not been reported. We, therefore, generated a gain-of-function transgenic mouse model that overexpresses Cul4A in a Cre-dependent manner. Before Cre recombination, these mice express LacZ during development in most adult tissues. After Cre-mediated excision of the LacZ reporter, the transfected Cul4A gene is expressed along with a C-terminal Myc-tag in different tissues. In this study, Cre-excision was induced in mouse lungs by inhalation of an adenovirus vector encoding Cre recombinase. This mouse model provides a valuable resource for investigating the significance of Cul4A overexpression in various tissues.
authors.affiliation: Thoracic Oncology Laboratory, Department of Surgery, Comprehensive Cancer Center, University of California, San Francisco, California 94143, USA.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.forename: Ming-Szu
authors.initials: MS
authors.lastname: Hung
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Mao
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Tan
authors.forename: Kenneth
authors.initials: K
authors.lastname: Jahan
authors.forename: Hannah
authors.initials: H
authors.lastname: Roos
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Xu
authors.forename: David M
authors.initials: DM
authors.lastname: Jablons
authors.forename: Liang
authors.initials: L
authors.lastname: You
mesh_terms: D000256:Adenoviridae; D000818:Animals; D044842:Cullin Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D019426:Integrases / Q000235:genetics* / Q000378:metabolism*; D007763:Lac Operon; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D023421:Models, Animal; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D019076:Transgenes
chemical_list: C491940:Cul4a protein, mouse; D044842:Cullin Proteins; C045073:Cre recombinase; D019426:Integrases