title: [Effects of EMPs on HUVECs' functions in vitro].
abstract: OBJECTIVE
  To investigate the effects of EMPs on HUVECs permeability in vitro, then evaluate the effect on the Avascular Necrosis of Femoral Head.
  
  
  METHODS
  Incubate HUVECs with EMPs which is released by HUVECs in vitro, then estimate the endothelial permeability.
  
  
  RESULTS
  Flow cytometry analysis showed that EMPs were released at maximum concentration(7.66 × 10(4)/ml) after 24 hours stimulated by 10 mmol DEX And 7.66 × 10(4), 7.66 × 10(3)/ml concentration (moderate and high concentrations) of EMPs caused injury to the permeability of HUVECs (P values are 0.002 and 0.031 respectively, < 0.05), while 7.66 × 10(2)/ml concentration (low concentration) of EMPs didn't (P = 0.425 > 0.05).
  
  
  CONCLUSIONS
  Dexamethasone can induce HUVECs to release EMPs in vitro. EMPs caused collapse of connections of HUVECs, and increase the HUVECs permeability, decrease vascular stability, finally lead to Avascular Necrosis of Femoral Head.
authors.affiliation: Department of Orthopaedics, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Min-Zhe
authors.initials: MZ
authors.lastname: Zheng
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wu
authors.forename: Chun-Liang
authors.initials: CL
authors.lastname: Ji
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Gui-Xing
authors.initials: GX
authors.lastname: Qiu
authors.forename: Xi-Sheng
authors.initials: XS
authors.lastname: Weng
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005271:Femur Head Necrosis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D014471:Umbilical Veins / Q000166:cytology