title: Control generating of bacterial magnetic nanoparticle-doxorubicin conjugates by poly-L-glutamic acid surface modification.
abstract: By using poly-L-glutamic acid (PLGA) to modify the membrane surface of bacterial magnetic nanoparticles (BMPs), (BMP)-doxorubicin conjugates (DBMP-P) could be control generated. The doxorubicin loading ratio could be raised up to 81.7% (w/w) in comparison with that of dual functional linkers. DBMP-P was characterized by transmission electron micrographs, attenuated total reflection infrared spectroscopy, magnetic properties, and dynamic light scattering. It is found that increase of the doxorubicin/PLGA modified BMP (PBMP) ratio leads to an increase of the drug loading ratio and a decrease of saturation magnetization. Besides, DBMP-P is sensitive to pH to facilitate drug release, shows enhancement of uptake by cancer cells, and is strongly cytotoxic to HePG2 and MCF-7 cells.
authors.affiliation: Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China. linguo@nju.edu.cn
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Ji
authors.initials: J
authors.lastname: Huang
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zheng
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D055944:Magnetosomes / Q000737:chemistry*; D042201:Magnetospirillum / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D011099:Polyglutamic Acid / Q000737:chemistry*
chemical_list: D000903:Antibiotics, Antineoplastic; D011099:Polyglutamic Acid; D004317:Doxorubicin