title: Bioflocculant production by Virgibacillus sp. Rob isolated from the bottom sediment of Algoa Bay in the Eastern Cape, South Africa.
abstract: A bioflocculant-producing marine bacterium previously isolated from marine sediment of Algoa Bay was screened for flocculant production. Comparative analysis of 16S rDNA sequence identified the isolate to have 99% similarity to Virgibacillus sp. XQ-1 and it was deposited in the GenBank as Virgibacillus sp. Rob with accession number HQ537127. The bacterium produced biflocculants optimally in glucose (70.4%) and peptone (70.4%) as sole sources of carbon and nitrogen, alkaline pH (12) (74%); and the presence of Fe2+ (74%). Chemical analysis of the bioflocculant revealed it to be a polysaccharide.
authors.affiliation: Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Sekelwa
authors.initials: S
authors.lastname: Cosa
authors.forename: Leonard V
authors.initials: LV
authors.lastname: Mabinya
authors.forename: Ademola O
authors.initials: AO
authors.lastname: Olaniran
authors.forename: Omobola O
authors.initials: OO
authors.lastname: Okoh
authors.forename: Kim
authors.initials: K
authors.lastname: Bernard
authors.forename: Shaun
authors.initials: S
authors.lastname: Deyzel
authors.forename: Anthony I
authors.initials: AI
authors.lastname: Okoh
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005427:Flocculation; D019015:Geologic Sediments; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D013019:South Africa; D058679:Virgibacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D017931:DNA Primers