title: Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.
abstract: Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.
authors.affiliation: Department of Immunotechnology, BMC D13, Lund University, Lund, Sweden.
authors.forename: Linda
authors.initials: L
authors.lastname: Dexlin-Mellby
authors.forename: Anna
authors.initials: A
authors.lastname: Sandström
authors.forename: Linn
authors.initials: L
authors.lastname: Antberg
authors.forename: Jenny
authors.initials: J
authors.lastname: Gunnarsson
authors.forename: Stefan R
authors.initials: SR
authors.lastname: Hansson
authors.forename: Carl A K
authors.initials: CA
authors.lastname: Borrebaeck
authors.forename: Christer
authors.initials: C
authors.lastname: Wingren
mesh_terms: D000906:Antibodies / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D014066:Palatine Tonsil / Q000166:cytology; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins; D015203:Reproducibility of Results; D014020:Tissue Extracts / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D000954:Antigens, Surface; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014020:Tissue Extracts