title: [Scanning electron microscopy observation of the growth of osteoblasts on Ti-24Nb-4Zr-8Sn modified by micro-arc oxidation and alkali-heat treatment and implant-bone interface].
abstract: OBJECTIVE
  To observe the efficacy of micro-arc oxidation and alkali-heat treatment (MAH) on Ti-24Nb-4Zr-8Sn (Ti2448).
  
  
  METHODS
  Disks (diameter of 14.5 mm, thickness of 1 mm) and cylinders (diameter of 3 mm, height of 10 mm) were fabricated from Ti2448 alloy. Samples were divided into three groups: polished (Ti2448), micro-arc oxidation(MAO-Ti2448), micro-arc oxidation and alkali-heat treatment (MAH-Ti2448). MC3T3-E1 osteoblastic cells were cultured on the disks and cell morphology was observed with scanning electron microscopy (SEM) aftre 3 days. The cylinder samples were implanted in the tibia of dogs and implant-bone interface was observed with SEM after 3 months.
  
  
  RESULTS
  A rough and porous structure was shown in both MAO and MAH group. The MC3T3-E1 cells on the MAH-Ti2448 discs spread fully in intimate contact with the underlying coarse surface through active cytoskeletal extentions. Osseointegration was formed in the implant-bone interface in MAH samples.
  
  
  CONCLUSIONS
  MAH treatment can provide a more advantageous Ti2448 surface to osteoblastic cells than MAO treatment does, and the former can improve the implant-bone integration.
authors.affiliation: Dental Institute, General Hospital of PLA, Beijing 100853, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
mesh_terms: D000468:Alkalies; D000497:Alloys / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D003722:Dental Alloys; D004285:Dogs; D006358:Hot Temperature; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology*; D010084:Oxidation-Reduction; D019736:Prostheses and Implants; D019919:Prosthesis Implantation; D013499:Surface Properties; D013977:Tibia / Q000601:surgery*
chemical_list: D000468:Alkalies; D000497:Alloys; D003722:Dental Alloys; C548656:Ti-24Nb-4Zr-7.9Sn alloy