title: Immobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro.
abstract: OBJECTIVE
  The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention.
  
  
  METHODS
  We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds.
  
  
  RESULTS
  Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs.
  
  
  CONCLUSIONS
  These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
authors.affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. jakubova@biomed.cas.cz
authors.forename: R
authors.initials: R
authors.lastname: Jakubova
authors.forename: A
authors.initials: A
authors.lastname: Mickova
authors.forename: M
authors.initials: M
authors.lastname: Buzgo
authors.forename: M
authors.initials: M
authors.lastname: Rampichova
authors.forename: E
authors.initials: E
authors.lastname: Prosecka
authors.forename: D
authors.initials: D
authors.lastname: Tvrdik
authors.forename: E
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000378:metabolism; D019902:Chondrocytes / Q000166:cytology*; D004337:Drug Carriers / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013552:Swine
chemical_list: D004337:Drug Carriers; D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; C016240:polycaprolactone