title: Nanoband electrode for high-performance in-channel amperometric detection in dual-channel microchip capillary electrophoresis.
abstract: A nanoband electrode detector integrated with a dual-channel polydimethylsiloxane microchip is proposed for in-channel amperometric detection in microchip capillary electrophoresis. Gold nanoband electrodes, which were fabricated on SU-8 substrates with a 100-nm-width gold layer, were introduced into the dual-channel microchip to be an electrochemical detector. Due to the nano-sized width of the detector, the noise of the amperometric detection was significantly reduced, and a high separation resolution was achieved for monitoring the analytes. The detection sensitivity of the system was improved by high signal-to-noise ratio, and a low detection limit on microchip was obtained for p-aminophenol (2.09 nM). Because of the high resolution in measuring half-peak width, the plate number that is used to evaluate the separation efficiency was 1.5-fold higher than that using 50-μm-width electrochemical detector. The effect of sample injection time and data acquisition time on separation efficiency was investigated, and an attractive separation efficiency was achieved with a plate number up to 17,500.
authors.affiliation: Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Chuanpin
authors.initials: C
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Teng
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Hahn
mesh_terms: D000627:Aminophenols / Q000032:analysis / Q000302:isolation & purification; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D036103:Nanotechnology / Q000295:instrumentation; D012680:Sensitivity and Specificity
chemical_list: D000627:Aminophenols; C026729:4-aminophenol