title: Production and characterization of exopolysaccharides and antioxidant from Paenibacillus sp. TKU023.
abstract: Using squid pen powder (SPP) as the sole C/N source, Paenibacillus sp. TKU023 produced exopolysaccharides (EPS) and antioxidant. With medium containing 1.5% SPP, 0.1% K(2)HPO(4), and 0.05% MgSO(4)·7H(2)O, pH 7.23, the culture was incubated at 37°C in liquid (50 mL/250 mL) for five days. The resultant culture supernatant had higher EPS productivity (4.55 g/L). The crude EPS were isolated by centrifugation, methanol precipitation and deproteinization. The characterization of the EPS demonstrated that it was mainly composed of glucose and maltose. In addition, the culture supernatant incubated for four days by using baffled base flask showed the strongest antioxidant activities and the highest total phenolic content, but maximum EPS production was found at the fifth day by using flat base flask. The production of two invaluable environmental-friendly biomaterials (EPS and antioxidant) is unprecedented. Besides, the use of SPP (waste) is green that made the whole process more valuable and attractive.
authors.affiliation: Department of Cosmetic Science and Application, Lan-Yang Institute of Technology, I-Lan 261, Taiwan.
authors.forename: Chuan-Lu
authors.initials: CL
authors.lastname: Wang
authors.forename: Tzu-Huang
authors.initials: TH
authors.lastname: Huang
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Liang
authors.forename: Chun-Yong
authors.initials: CY
authors.lastname: Fang
authors.forename: San-Lang
authors.initials: SL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D049832:Decapodiformes / Q000737:chemistry; D056507:Paenibacillus / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D003470:Culture Media; D011135:Polysaccharides, Bacterial