title: Low-SDS Blue native PAGE.
abstract: SDS normally is strictly avoided during Blue native (BN) PAGE because it leads to disassembly of protein complexes and unfolding of proteins. Here, we report a modified BN-PAGE procedure, which is based on low-SDS treatment of biological samples prior to native gel electrophoresis. Using mitochondrial OXPHOS complexes from Arabidopsis as a model system, low SDS concentrations are shown to partially dissect protein complexes in a very defined and reproducible way. If combined with 2-D BN/SDS-PAGE, generated subcomplexes and their subunits can be systematically investigated, allowing insights into the internal architecture of protein complexes. Furthermore, a 3-D BN/low-SDS BN/SDS-PAGE system is introduced to facilitate structural analysis of individual protein complexes without their previous purification.
authors.affiliation: Institute for Plant Genetics, Faculty of Natural Sciences, Leibniz Universität Hannover, Hannover, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Klodmann
authors.forename: Dagmar
authors.initials: D
authors.lastname: Lewejohann
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Braun
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000032:analysis* / Q000302:isolation & purification; D042967:Electron Transport Complex I / Q000032:analysis* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D024101:Mitochondrial Proteins / Q000032:analysis* / Q000302:isolation & purification; D020543:Proteome / Q000032:analysis / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; D020543:Proteome; D042967:Electron Transport Complex I