title: Methyl-coenzyme M reductase from Methanothermobacter marburgensis.
abstract: Methyl-coenzyme M reductase catalyzes the reversible synthesis of methane from methyl-coenzyme M in methanogenic and ANME-1 and ANME-2 Archaea. The purification procedure for methyl-coenzyme M reductase from Methanothermobacter marburgensis is described. The procedure is an accumulation of almost 30 years of research on MCR starting with the first purification described by Ellefson and Wolfe (Ellefson, W.L., and Wolfe, R.S. (1981). Component C of the methylreductase system of Methanobacterium. J. Biol. Chem.256, 4259-4262). To provide a context for this procedure, some background information is provided, including a description of whole cell experiments that provided much of our knowledge of the behavior and properties of methyl-coenzyme M reductase.
authors.affiliation: Department of Chemistry and Biochemistry, Auburn University, Alabama, USA.
authors.forename: Evert C
authors.initials: EC
authors.lastname: Duin
authors.forename: Divya
authors.initials: D
authors.lastname: Prakash
authors.forename: Charlene
authors.initials: C
authors.lastname: Brungess
mesh_terms: D015080:Mesna / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D017013:Methanobacteriaceae / Q000201:enzymology* / Q000254:growth & development; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D010769:Phosphothreonine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
chemical_list: C049349:7-mercaptoheptanoylthreonine phosphate; D010769:Phosphothreonine; C023349:methyl coenzyme M; D010088:Oxidoreductases; C023895:methyl coenzyme M methylreductase; D015080:Mesna