title: Multi-modal molecular imaging approaches to detect primary cells in preclinical models.
abstract: The need to understand cellular trafficking in vivo in situ requires the development and application of novel methodologies for cellular labeling and cell tracking. Here we applied new technologies associated with advances in molecular imaging to demonstrate the feasibility of labeling primary immune cells. We demonstrate the utility of fluorescently tagged polystyrene microspheres, MRI susceptible emulsions and cell entry peptoids. The adaptation of these labeling agents will permit cell specific delivery, diagnostic sensing and the delivery of therapeutic agents to sites of inflammation and infection.
authors.affiliation: MRC Centre for Inflammation Research, Queen's Medical Research Centre, University of Edinburgh, 47 Little France Crescent, Edinburgh, UK. kdhaliwa@staffmail.ed.ac.uk
authors.forename: Kevin
authors.initials: K
authors.lastname: Dhaliwal
authors.forename: Lois
authors.initials: L
authors.lastname: Alexander
authors.forename: Geraldine
authors.initials: G
authors.lastname: Escher
authors.forename: Asier
authors.initials: A
authors.lastname: Unciti-Broceta
authors.forename: Maurits
authors.initials: M
authors.lastname: Jansen
authors.forename: Neil
authors.initials: N
authors.lastname: Mcdonald
authors.forename: Juan M
authors.initials: JM
authors.lastname: Cardenas-Maestre
authors.forename: Rosario
authors.initials: R
authors.lastname: Sanchez-Martin
authors.forename: John
authors.initials: J
authors.lastname: Simpson
authors.forename: Chris
authors.initials: C
authors.lastname: Haslett
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D058948:Cell Tracking / Q000379:methods*; D008264:Macrophages / Q000166:cytology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008863:Microspheres; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*