title: Dextran sulphate sodium increases splenic Gr1(+)CD11b(+) cells which accelerate recovery from colitis following intravenous transplantation.
abstract: While Gr1(+)CD11b(+) cells are known to regulate immune responses and accumulate in most cancer tissues, the function of Gr1(+)CD11b(+) cells in inflammation is poorly understood. We investigated the role of Gr1(+)CD11b(+) cells in a dextran sulphate sodium (DSS)-treated mouse model of ulcerative colitis (UC). C57BL/6 mice were treated with 2% DSS in drinking water for 5 days. Disease progression and recovery were assessed by body weight, disease activity index score (DAI) score and colon length. Splenic Gr1(+)CD11b(+) cell number was greatly increased during the recovery phase of DSS-induced colitis. DSS-derived splenic Gr1(+)CD11b(+) cells were administered intravenously to recipient (C57BL/6) mice during the early phase of DSS treatment. The transplanted splenic DSS-induced Gr1(+)CD11b(+) cells improved DSS-induced colitis and promoted efficient colonic mucosal healing. We found that the CD11b(+) single positive cells increased in the course of DSS-induced colitis in lamina propria. The transplantation of splenic Gr1(+)CD11b(+) cells induced feedback suppression of myeloid-lineage cell development. Namely, the transplantation of splenic Gr1(+)CD11b(+) cells greatly suppressed the migration of CD11b(+) single positive cells to the lamina propria. Further, transplantation of Gr-1(+)CD11b(+) cells greatly suppressed the increase of the same population, especially during the late phase of DSS colitis both in spleen and bone marrow.
authors.affiliation: Department of Immunology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Aichi, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Nishio
authors.forename: Z
authors.initials: Z
authors.lastname: Cheng
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: K-I
authors.initials: KI
authors.lastname: Isobe
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000096:biosynthesis; D002452:Cell Count; D002454:Cell Differentiation; D002465:Cell Movement; D017690:Cell Transplantation; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000276:immunology* / Q000503:physiopathology / Q000628:therapy*; D003106:Colon / Q000473:pathology*; D016264:Dextran Sulfate / Q000008:administration & dosage; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D039481:CD11b Antigen; D011956:Receptors, Cell Surface; C551746:granulocyte receptor 1, mouse; D016264:Dextran Sulfate