title: DLBS1033, a protein extract from Lumbricus rubellus, possesses antithrombotic and thrombolytic activities.
abstract: The medicinal value of earthworm has been widely known since the history of Asian ancient medicine. This present study aims to determine the mechanism of action and effect of a standardized extract of Lumbricus rubellus named as DLBS1033. The fibrinogen degradation, antiplatelet aggregation, and ex vivo antithrombotic assay using human blood were performed to study antithrombotic activity. Fibrin plate and clot lysis assay were also done to examine thrombolytic properties. DLBS1033 was found to possess fibrinogenolytic activity on α-, β-, and γ-chain of fibrinogen. It also induced antiplatelet aggregation and prolonged blood clotting time, which further confirmed its antithrombotic properties. In addition, thrombolytic properties of DLBS1033 were shown with its fast and long-acting fibrinolytic activity, as well as its effective blood clot lysis activities. In conclusion, DLBS1033 conferred antithrombotic and thrombolytic action which could be used as a safe and promising oral thrombolytic drug.
authors.affiliation: Division of Protein Biochemistry and Molecular Pharmacology, Dexa Laboratories of Biomolecular Sciences, PT Dexa Medica, Industri Selatan V Block PP no. 7, Kawasan Industri Jababeka II, Cikarang 17550, Indonesia.
authors.forename: Jessica
authors.initials: J
authors.lastname: Trisina
authors.forename: Febrina
authors.initials: F
authors.lastname: Sunardi
authors.forename: Maggy T
authors.initials: MT
authors.lastname: Suhartono
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Tjandrawinata
mesh_terms: D000818:Animals; D005337:Fibrin / Q000187:drug effects / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D009835:Oligochaeta / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: C559131:DLBS 1033; D005343:Fibrinolytic Agents; D014020:Tissue Extracts; D005337:Fibrin