title: Aminohexyl-sepharose affinity chromatography : purification of an auxin receptor.
abstract: Affinity chromatography is a widely used technique that is based on specific binding, usually between proteins and low-molecular weight ligands. It is often found that the presence of a spacer arm between the supporting matrix and the affinity ligand is beneficial to binding capacity. A convenient starting material for affinity chromatography involving carboxyl-containing ligands is aminohexyl-Sepharose, which is commercially available and contains a spacer arm; any carboxyl-containing ligand can easily be coupled to the amino group of the resin. As an example of the use of aminohexyl-Sepharose, this chapter describes the purification of a soluble auxin receptor using an affinity resin produced by derivatization of aminohexyl-Sepharose.
authors.affiliation: School of Life Sciences, Leicester Polytechnic, Leicester, UK.
authors.forename: R D
authors.initials: RD
authors.lastname: Barker
authors.forename: H M
authors.initials: HM
authors.lastname: Bailey