title: Normal and disordered reticulocyte maturation.
abstract: OBJECTIVE
  Reticulocyte remodeling has emerged as an important model for the understanding of vesicular trafficking and selective autophagy in mammalian cells. This review covers recent advances in our understanding of these processes in reticulocytes and the role of these processes in erythroid development.
  
  
  RESULTS
  Enucleation is caused by the coalescence of vesicles at the nuclear-cytoplasmic junction and microfilament contraction. Mitochondrial elimination is achieved through selective autophagy, in which mitochondria are targeted to autophagosomes, and undergo subsequent degradation and exocytosis. The mechanism involves an integral mitochondrial outer membrane protein and general autophagy pathways. Plasma membrane remodeling, and the elimination of certain intracellular organelles occur through the exosomal pathway.
  
  
  CONCLUSIONS
  Vesicular trafficking and selective autophagy have emerged as central processes in cellular remodeling. In reticulocytes, this includes enucleation and the elimination of all membrane-bound organelles and ribosomes. Ubiquitin-like conjugation pathways, which are required for autophagy in yeast, are not essential for mitochondrial clearance in reticulocytes. Thus, in higher eukaryotes, there appears to be redundancy between these pathways and other processes, such as vesicular nucleation. Future studies will address the relationship between autophagy and vesicular trafficking, and the significance of both for cellular remodeling.
authors.affiliation: Department of Biochemistry, St Jude Children's Research Hospital, Memphis, Tennessee, USA. paul.ney@stjude.org
authors.forename: Paul A
authors.initials: PA
authors.lastname: Ney
mesh_terms: D000818:Animals; D001343:Autophagy; D004920:Erythropoiesis; D006801:Humans; D012156:Reticulocytes / Q000166:cytology* / Q000378:metabolism / Q000473:pathology*