title: Xeno-repopulation of Fah -/- Nod/Scid mice livers by human hepatocytes.
abstract: Functional human hepatocytes xenografted into the liver of mice can be used as a model system to study pharmacokinetics, infection of hepatitis viruses, and the efficacy of hepatitis vaccines. Significant levels of liver xeno-repopulation have been reported in Fah (-/-) Rag2 (-/-) Il2rg (-/-) mice. However, A new model, termed Fah (-/-) Nod/Scid mice, which combines the advantages of liver repopulation in Fah (-/-) mice with the ease of xenotransplantation in Nod/Scid mice was obtained by gradual cross-breeding. Fah (-/-) Nod/Scid mice were easily maintained in breeding colonies and in adult animal care facilities. FK506 treatment combined with gradual withdrawal of NTBC before cell transplantation ensured that Fah (-/-) Nod/Scid mice were susceptible to liver xeno-repopulation by human hepatocytes; the proportion of engrafted human hepatocytes reached 33.6%. The function of the expanded human hepatocytes within the chimeric liver was confirmed by weight curve analysis, the expression of characteristic proteins, and the biochemical analysis of liver function. These results show that Fah (-/-) Nod/Scid mice are an ideal humanized liver mouse model with many useful applications.
authors.affiliation: Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Baoliang
authors.initials: B
authors.lastname: Su
authors.forename: Changcheng
authors.initials: C
authors.lastname: Liu
authors.forename: Dao
authors.initials: D
authors.lastname: Xiang
authors.forename: Haibin
authors.initials: H
authors.lastname: Zhang
authors.forename: Siming
authors.initials: S
authors.lastname: Yuan
authors.forename: Minjun
authors.initials: M
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Haiying
authors.initials: H
authors.lastname: Zhu
authors.forename: Zhiying
authors.initials: Z
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D006801:Humans; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000166:cytology*; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D016559:Tacrolimus / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D006867:Hydrolases; C020156:fumarylacetoacetase; D016559:Tacrolimus