title: The DFPase from Loligo vulgaris in sugar surfactant-based bicontinuous microemulsions: structure, dynamics, and enzyme activity.
abstract: The enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is of great interest because of its ability to catalyze the hydrolysis of highly toxic organophosphates. In this work, the enzyme structure in solution (native state) was studied by use of different scattering methods. The results are compared with those from hydrodynamic model calculations based on the DFPase crystal structure. Bicontinuous microemulsions made of sugar surfactants are discussed as host systems for the DFPase. The microemulsion remains stable in the presence of the enzyme, which is shown by means of scattering experiments. Moreover, activity assays reveal that the DFPase still has high activity in this complex reaction medium. To complement the scattering experiments cryo-SEM was also employed to study the microemulsion structure.
authors.affiliation: TU Berlin, Institut für Chemie, Stranski-Laboratorium für Physikalische und Theoretische Chemie, Straße des 17.Juni 124, 10623 Berlin, Germany. s.wellert@tu-berlin.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Wellert
authors.forename: Brigtte
authors.initials: B
authors.lastname: Tiersch
authors.forename: Joachim
authors.initials: J
authors.lastname: Koetz
authors.forename: André
authors.initials: A
authors.lastname: Richardt
authors.forename: Alain
authors.initials: A
authors.lastname: Lapp
authors.forename: Olaf
authors.initials: O
authors.lastname: Holderer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Gäb
authors.forename: Marc-Michael
authors.initials: MM
authors.lastname: Blum
authors.forename: Christoph
authors.initials: C
authors.lastname: Schulreich
authors.forename: Ralf
authors.initials: R
authors.lastname: Stehle
authors.forename: Thomas
authors.initials: T
authors.lastname: Hellweg
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry / Q000494:pharmacology*; D004655:Emulsions; D049834:Loligo / Q000201:enzymology / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D033363:Neutron Diffraction; D044345:Phosphoric Triester Hydrolases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053838:Scattering, Small Angle; D012996:Solutions / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002241:Carbohydrates; D004655:Emulsions; D012996:Solutions; D013501:Surface-Active Agents; D044345:Phosphoric Triester Hydrolases; C020061:diisopropyl-fluorophosphatase