title: Control of GABA Release at Mossy Fiber-CA3 Connections in the Developing Hippocampus.
abstract: In this review some of the recent work carried out in our laboratory concerning the functional role of GABAergic signalling at immature mossy fibres (MF)-CA3 principal cell synapses has been highlighted. While in adulthood MF, the axons of dentate gyrus granule cells release onto CA3 principal cells and interneurons glutamate, early in postnatal life they release GABA, which exerts into targeted cells a depolarizing and excitatory action. We found that GABA(A)-mediated postsynaptic currents (MF-GPSCs) exhibited a very low probability of release, were sensitive to L-AP4, a group III metabotropic glutamate receptor agonist, and revealed short-term frequency-dependent facilitation. Moreover, MF-GPSCs were down regulated by presynaptic GABA(B) and kainate receptors, activated by spillover of GABA from MF terminals and by glutamate present in the extracellular medium, respectively. Activation of these receptors contributed to the low release probability and in some cases to synapses silencing. By pairing calcium transients, associated with network-driven giant depolarizing potentials or GDPs (a hallmark of developmental networks thought to represent a primordial form of synchrony between neurons), generated by the synergistic action of glutamate and GABA with MF activation increased the probability of GABA release and caused the conversion of silent synapses into conductive ones suggesting that GDPs act as coincident detector signals for enhancing synaptic efficacy. Finally, to compare the relative strength of CA3 pyramidal cell output in relation to their MF glutamatergic or GABAergic inputs in adulthood or in postnatal development, respectively, a realistic model was constructed taking into account different biophysical properties of these synapses.
authors.affiliation: Department of Neurobiology, International School for Advanced Studies Trieste, Italy.
authors.forename: Victoria F
authors.initials: VF
authors.lastname: Safiulina
authors.forename: Maddalena D
authors.initials: MD
authors.lastname: Caiati
authors.forename: Sudhir
authors.initials: S
authors.lastname: Sivakumaran
authors.forename: Giacomo
authors.initials: G
authors.lastname: Bisson
authors.forename: Michele
authors.initials: M
authors.lastname: Migliore
authors.forename: Enrico
authors.initials: E
authors.lastname: Cherubini