title: Improved resistance against Botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production.
abstract: Bacteria such as Pantoea agglomerans (Pa-AF2), Bacillus subtilis (Bs-271), Acinetobacter lwoffii (Al-113), and Pseudomonas fluorescens (Pf-CT2), originating from the vineyard, can induce defense responses and enhance resistance of grapevine against the fungal pathogen Botrytis cinerea. The perception of these bacteria by plant cells or tissues in relation to their activities remains unknown. In this study, we examined the relationships between the activity of each bacterium to induce or prime some defense responses, and its effectiveness to induce resistance in grapevine against B. cinerea. We showed that all selected bacteria are capable of inducing early oxidative burst and phytoalexin (trans-resveratrol and trans-ε-viniferin) production in grapevine cells and leaves. Pf-CT2 and Al-113 induced higher H(2)O(2) and trans-resveratrol accumulations, and were able to further prime plants for accelerated phytoalexin production after B. cinerea challenge. These two bacteria were also the most effective in inducing local and systemic resistance. A similar level of induced resistance was observed with live Pa-AF2 which also induced but not primed a greater accumulation of trans-resveratrol. However, Bs-271, which was less effective in inducing resistance, induced a lower trans-resveratrol synthesis, without priming activity. Treatment of grapevine cells with growing medium or crude extract of the bacteria quickly and strongly enhanced oxidative burst compared with the live bacteria. However, both treatments resulted in comparable amounts of phytoalexins and induced local and systemic resistance to B. cinerea as compared with those induced by living bacteria, with extracts from Pf-CT2 and Al-113 being the most effective. Together, these results indicate that induced resistance can be improved by treatment with bacteria or derived compounds which induced or primed plants for enhanced phytoalexin accumulation.
authors.affiliation: Plantes Pesticides & développement Durable, University of Reims, F-51687 Reims cedex 2, France.
authors.forename: Bas
authors.initials: B
authors.lastname: Verhagen
authors.forename: Patricia
authors.initials: P
authors.lastname: Trotel-Aziz
authors.forename: Philippe
authors.initials: P
authors.lastname: Jeandet
authors.forename: Fabienne
authors.initials: F
authors.lastname: Baillieul
authors.forename: Aziz
authors.initials: A
authors.lastname: Aziz
mesh_terms: D000150:Acinetobacter / Q000502:physiology; D001412:Bacillus subtilis / Q000502:physiology; D020171:Botrytis / Q000276:immunology / Q000472:pathogenicity*; D016923:Cell Death / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D020636:Pantoea / Q000502:physiology; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D057865:Plant Immunity / Q000276:immunology; D018515:Plant Leaves / Q000276:immunology / Q000382:microbiology; D018517:Plant Roots / Q000276:immunology / Q000382:microbiology; D011551:Pseudomonas fluorescens / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000276:immunology; D000077185:Resveratrol; D012717:Sesquiterpenes / Q000378:metabolism*; D015398:Signal Transduction; D013267:Stilbenes; D027843:Vitis / Q000276:immunology* / Q000382:microbiology*; D000094162:Phytoalexins
chemical_list: D017382:Reactive Oxygen Species; D012717:Sesquiterpenes; D013267:Stilbenes; D006861:Hydrogen Peroxide; D000077185:Resveratrol; D000094162:Phytoalexins