title: Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling.
abstract: In a genetic screen we isolated mutations in CG10260, which encodes a phosphatidylinositol 4-kinase (PI4KIIIalpha), and found that PI4KIIIalpha is required for Hippo signaling in Drosophila ovarian follicle cells. PI4KIIIalpha mutations in the posterior follicle cells lead to oocyte polarization defects similar to those caused by mutations in the Hippo signaling pathway. PI4KIIIalpha mutations also cause misexpression of well-established Hippo signaling targets. The Merlin-Expanded-Kibra complex is required at the apical membrane for Hippo activity. In PI4KIIIalpha mutant follicle cells, Merlin fails to localize to the apical domain. Our analysis of PI4KIIIalpha mutants provides a new link in Hippo signal transduction from the cell membrane to its core kinase cascade.
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Natalie
authors.initials: N
authors.lastname: Denef
authors.forename: Charm
authors.initials: C
authors.lastname: Tang
authors.forename: Trudi
authors.initials: T
authors.lastname: Schüpbach
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D016764:Cell Polarity / Q000235:genetics*; D029721:Drosophila Proteins / Q000378:metabolism* / Q000502:physiology; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005260:Female; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism* / Q000502:physiology; D015778:Minor Histocompatibility Antigens; D009154:Mutation / Q000502:physiology; D025581:Neurofibromin 2 / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D010641:Phenotype; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D000099317:Hippo Kinases
chemical_list: D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D015778:Minor Histocompatibility Antigens; D025581:Neurofibromin 2; D017853:Phosphotransferases (Alcohol Group Acceptor); D017346:Protein Serine-Threonine Kinases; C099885:merlin, Drosophila; C402182:phosphatidylinositol phosphate 4-kinase; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases