title: Flexible polymeric ultrathin film for mesenchymal stem cell differentiation.
abstract: Ultrathin films (also called nanofilms) are two-dimensional (2-D) polymeric structures with potential application in biology, biotechnology, cosmetics and tissue engineering. Since they can be handled in liquid form with micropipettes or tweezers they have been proposed as flexible systems for cell adhesion and proliferation. In particular, with the aim of designing a novel patch for bone or tendon repair and healing, in this work the biocompatibility, adhesion and proliferation activity of Saos-2, MRC-5 and human and rat mesenchymal stem cells on poly(lactic acid) nanofilms were evaluated. The nanofilms did not impair the growth and differentiation of osteoblasts and chondrocytes. Moreover, nanofilm adhesion to rabbit joints was evident under ex vivo conditions.
authors.affiliation: Istituto Italiano di Tecnologia, Center for MicroBioRobotics @ SSSA, Pontedera, Italy. virginia.pensabene@iit.it
authors.forename: Virginia
authors.initials: V
authors.lastname: Pensabene
authors.forename: Silvia
authors.initials: S
authors.lastname: Taccola
authors.forename: Leonardo
authors.initials: L
authors.lastname: Ricotti
authors.forename: Gianni
authors.initials: G
authors.lastname: Ciofani
authors.forename: Arianna
authors.initials: A
authors.lastname: Menciassi
authors.forename: Francesca
authors.initials: F
authors.lastname: Perut
authors.forename: Manuela
authors.initials: M
authors.lastname: Salerno
authors.forename: Paolo
authors.initials: P
authors.lastname: Dario
authors.forename: Nicola
authors.initials: N
authors.lastname: Baldini
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)