title: Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.
abstract: Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries.
authors.affiliation: Human Genetics Laboratory, Imperial Cancer Research Fund, Institute of Molecu1ar Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Monaco
authors.forename: Z
authors.initials: Z
authors.lastname: Lark
authors.forename: H
authors.initials: H
authors.lastname: Lehrach