title: Effect of a mixed herb-enriched diet on the innate immune response and disease resistance of Paralichthys olivaceus against Philasterides dicentrarchi infection.
abstract: We investigated the effect of a mixed herb-enriched diet obtained from pomegranate Punica granatum, Dalmatian chrysanthemum Chrysanthemum cinerariaefolium, and mastic-leaved prickly-ash Zanthoxylum schinifolium on innate immune mechanisms (e.g., phagocytosis activity, respiratory burst activity, alternative complement activity, lysozyme activity, and disease resistance) of olive flounder Paralichthys olivaceus against the scuticociliate Philasterides dicentrarchi. All experimental groups were challenged with P. dicentrarchi (1 x 10(5) ciliates/mL) through intraperitoneal administration of the pathogen (50 microL) on day 1. On day 7, the infected groups were fed 0, 5, 50, and 100 mg/kg of the enriched diets. The innate immune parameters, cumulative mortality, and relative percent survival (RPS) were assessed at weeks 1, 2, 3, and 4. Administration of 50 or 100 mg/kg of the herbal-enriched diet enhanced immunity throughout the experimental period. However, at the 5-mg/kg dosage, the enriched diet did not enhance the innate immune estimates at any time. At doses of 50 and 100 mg/kg, administration of the diet preceding the challenge with P. dicentrarchi decreased the percentage cumulative mortality in the experimental groups and thereby increased RPS values. This study reports that administration of 50 or 100 mg/kg mixed herbal-enriched diet could positively influence the innate immune response to P. dicentrarchi and enhance the health status of olive flounder with respect to this microbe.
authors.affiliation: Marine Applied Microbes and Aquatic Organism Disease Control Laboratory, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, South Korea.
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Harikrishnan
authors.forename: Chellam
authors.initials: C
authors.lastname: Balasundaram
authors.forename: Man-Chul
authors.initials: MC
authors.lastname: Kim
authors.forename: Ju-Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Han
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Heo
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D011723:Chrysanthemum cinerariifolium / Q000737:chemistry*; D016798:Ciliophora / Q000276:immunology; D016770:Ciliophora Infections / Q000517:prevention & control / Q000662:veterinary*; D004032:Diet / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000469:parasitology / Q000517:prevention & control; D005432:Flounder; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts