title: Preparation of C5-functionalized locked nucleic acids (LNAs).
abstract: Relative to conventional locked nucleic acids (LNAs), C5-functionalized LNAs with small entities at the C5-position display markedly higher (1) duplex thermostability with complementary single-stranded DNA/RNA targets, (2) target specificity, and (3) 3'-exonuclease stability. C5-Functionalized LNAs carrying a polarity-sensitive fluorophore enable sensitive and efficient discrimination of single nucleotide polymorphisms (SNPs) under non-stringent conditions. This unit describes protocols for chemical synthesis of an LNA uridine diol and corresponding C5-functionalized LNA uridine phosphoramidites. A procedure for incorporation of C5-functionalized LNA uridine phosphoramidites into oligodeoxyribonucleotides by automated DNA synthesis is also described.
authors.affiliation: University of Idaho, Moscow, Idaho, USA.
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
authors.forename: Michael E
authors.initials: ME
authors.lastname: Østergaard
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hrdlicka
mesh_terms: D000438:Alcohols / Q000737:chemistry; D004355:Drug Stability; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D014529:Uridine / Q000031:analogs & derivatives / Q000737:chemistry*
chemical_list: D000438:Alcohols; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C477371:locked nucleic acid; C101323:uridine phosphoramidite; D014529:Uridine