title: Huaishuia halophila gen. nov., sp. nov., isolated from coastal seawater.
abstract: A novel Gram-negative, non-motile bacterium, designated ZXM137(T), was isolated from seawater collected from a coastal region of Qingdao, China, during a massive green algae (Enteromorpha prolifera) bloom. Strain ZXM137(T) was strictly aerobic and did not accumulate poly-β-hydroxybutyrate. Growth occurred with 0.5-11.0% (w/v) NaCl, at pH 6-9 (optimum of pH 7) and at 4-45 °C (optimum at 28 °C). It contained Q-10 as the predominant ubiquinone and the major polar lipids were phosphatidylglycerol, phospholipids, and an unidentified aminolipid and lipid. The major cellular fatty acids of strain ZXM137(T) were C(18:1)ω7c, C(18:1)ω6c and 11-methyl C(18:1)ω7c. The DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZXM137(T) formed a distinct evolutionary lineage within the Roseobacter group in the class Alphaproteobacteria. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain ZXM137(T) represents a novel species in a new genus, for which the name Huaishuia halophila gen. nov., sp. nov. is proposed; the type strain is ZXM137(T) (=CGMCC 1.8891(T)=LMG 24854(T)).
authors.affiliation: Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Yan
authors.affiliation: Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Zizhong
authors.initials: Z
authors.lastname: Qi
authors.affiliation: SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000332:Aerobiosis; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041904:Roseobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D000094122:Polyhydroxybutyrates
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates