title: Host cell targets of tegument protein VP22 of herpes simplex virus 1.
abstract: In the present study, seven novel host cell factors associated with VP22 were identified from the human leukocyte cDNA library using a yeast two-hybrid high-throughput screening system. To confirm some of the interactions, VP22 and its homologues and two candidate targets were tagged with enhanced cyan or yellow fluorescent protein. While RING finger protein 10 (RNF10), WD repeat-containing protein 42A (WDR42A) or VP22 alone showed distinct subcellular localization patterns, RNF10 and WDR42A were relocated when co-expressed with VP22 or its homologues. Thus, these potential host cell factors of VP22 might expand the list of the host targets of VP22.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Meili
authors.initials: M
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ren
authors.forename: Chunfu
authors.initials: C
authors.lastname: Zheng
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes; D015870:Gene Expression; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D054884:Host-Pathogen Interactions; D006801:Humans; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D015678:Viral Structural Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D005456:Fluorescent Dyes; C406487:RNF10 protein, human; D015678:Viral Structural Proteins; C559948:WDR42 protein, human; C073413:herpes simplex virus type 1 protein VP22