title: Controlling the magnetic field distribution on the micrometer scale and generation of magnetic bead patterns for microfluidic applications.
abstract: As is well known, controlling the local magnetic field distribution on the micrometer scale in a microfluidic chip is significant and has many applications in bioanalysis based on magnetic beads. However, it is a challenge to tailor the magnetic field introduced by external permanent magnets or electromagnets on the micrometer scale. Here, we demonstrated a simple approach to controlling the local magnetic field distribution on the micrometer scale in a microfluidic chip by nickel patterns encapsulated in a thin poly(dimethylsiloxane) (PDMS) film under the fluid channel. With the precisely controlled magnetic field, magnetic bead patterns were convenient to generate. Moreover, two kinds of fluorescent magnetic beads were patterned in the microfluidic channel, which demonstrated that it was possible to generate different functional magnetic bead patterns in situ, and could be used for the detection of multiple targets. In addition, this method was applied to generate cancer cell patterns.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yu
authors.forename: Xuan
authors.initials: X
authors.lastname: Feng
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
mesh_terms: D006801:Humans; D008280:Magnetics; D044085:Microfluidics / Q000379:methods*; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D009369:Neoplasms / Q000473:pathology
chemical_list: D015335:Molecular Probes