title: Capillary electrophoresis microchip for direct amperometric detection of DNA fragments.
abstract: Detection and quantitation of nucleic acids have gained much importance in the last couple of decades, especially in the post-human genome project era. Such processes are tedious, time consuming and require expensive reagents and equipment. Therefore, in the present study, we demonstrated a simple process for the separation and analysis of small DNA fragments using capillary electrophoretic amperometric detection on an inexpensive disposable glass microchip. The device used polydimethylsiloxane engraved microchannel and Au/Ti in-channel microelectrodes for sample detection. The DNA fragments were separated under low electric field (20 V/cm) for improved detection sensitivity and to retain the biomolecules in their native conformation. With a low sample requirement (as low as 1 μL) and high reproducibility, the proposed microchip device was successful in resolution and detection of DNA fragments of various lengths.
authors.affiliation: Department of Nano Science and Engineering, Myongji University, Gyeonggi, Korea.
authors.forename: You-Cheol
authors.initials: YC
authors.lastname: Jang
authors.forename: Sandeep Kumar
authors.initials: SK
authors.lastname: Jha
authors.forename: Rohit
authors.initials: R
authors.lastname: Chand
authors.forename: Kamrul
authors.initials: K
authors.lastname: Islam
authors.forename: Yong-Sang
authors.initials: YS
authors.lastname: Kim
mesh_terms: D010582:Bacteriophage lambda / Q000737:chemistry / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D046209:Electrophoresis, Microchip / Q000379:methods*; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D009705:Nucleosides; D004247:DNA