title: Detection of honey bee (Apis mellifera) viruses with an oligonucleotide microarray.
abstract: In recent years, declines in honey bee (Apis mellifera L.) colonies have been observed to varying degrees worldwide with the worst losses in the USA being termed Colony Collapse Disorder (CCD). Pathogen load and the prevalence of honey bee viruses have been implicated in these losses and many diseased hives have multiple viruses present. We have designed and tested an oligonucleotide microarray which enables the simultaneous detection of nine honey bee viruses: Acute bee paralysis virus, Black queen cell virus, Chronic bee paralysis virus, Deformed wing virus, Kashmir bee virus, Sacbrood virus, Israel acute paralysis virus, Varroa destructor virus 1 and Slow paralysis virus. The microarray can be used to robustly diagnose nine viruses in one test.
authors.affiliation: Crop and Food Security Programme, Food and Environment Research Agency, Sand Hutton, York, United Kingdom. rachel.glover@fera.gsi.gov.uk
authors.forename: Rachel H
authors.initials: RH
authors.lastname: Glover
authors.forename: Ian P
authors.initials: IP
authors.lastname: Adams
authors.forename: Giles
authors.initials: G
authors.lastname: Budge
authors.forename: Selwyn
authors.initials: S
authors.lastname: Wilkins
authors.forename: Neil
authors.initials: N
authors.lastname: Boonham
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D056631:Colony Collapse / Q000821:virology*; D007304:Insect Viruses / Q000235:genetics / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D014481:United States