title: Study of adult neurogenesis in the Gallotia galloti lizard during different seasons.
abstract: In a previous study we found a seasonal distribution of cell proliferation (the first stage of adult neurogenesis) in the telencephalic ventricular walls of the adult Gallotia galloti lizard. The aim of the present work was to determine the influence of seasonality on the subsequent migration of the resulting immature neurons. We used wild animals injected with bromodeoxyuridine and kept in captivity within 30 days. To confirm the neuronal identity of these cells, we used double immunohistochemical 5-bromo-2'-deoxyuridine (BrdU) and doublecortin (DCX, an early neuronal marker) labeling, as well as autoradiography after the administration of methyl-[³H]thymidine ([³H]T). We found that: (1) the rate of cell division and/or migration from the ventricular walls varied with the season, especially in regions related with olfaction. (2) Immature neuron-like cells appeared to migrate in an apparently radial and tangential way towards different parts of the telencephalic parenchyma. (3) We did not observe ultrastructurally mature neurons until at least 90 days later, a period considerably greater than that reported for other species of vertebrates in similar studies.
authors.affiliation: Departamento de Psicobiología y Metodología de las Ciencias del Comportamiento, Facultad de Psicología, Universidad de La Laguna, La Laguna, Santa Cruz de Tenerife 38071, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Delgado-Gonzalez
authors.forename: S
authors.initials: S
authors.lastname: Gonzalez-Granero
authors.forename: C M
authors.initials: CM
authors.lastname: Trujillo-Trujillo
authors.forename: J M
authors.initials: JM
authors.lastname: García-Verdugo
authors.forename: M C
authors.initials: MC
authors.lastname: Damas-Hernandez
mesh_terms: D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D008116:Lizards / Q000254:growth & development*; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology*; D012621:Seasons; D013687:Telencephalon / Q000166:cytology* / Q000254:growth & development*