title: [Usefulness of real-time PCR in detecting Chlamydia trachomatis and Neisseria gonorrhoeae in endocervical swabs and first-voided urine specimens].
abstract: We evaluated performance of Abbott RealTime CT/NG assay (real-time PCR, Abbott Japan) for detect Chlamydia trachomatis and Neisseria gonorrhoeae by real-time PCR in 88 female patients with cervicitis symptoms seen at gynecological clinics and 100 male patients with urethritis symptoms seen at urological or dermatology clinics in Kitakyushu, Japan. Endocervical swab and first-voided urine (FVU) specimens were then collected from women and FVU specimens from men. Detection rates of C. trachomatis and N. gonorrhoeae by real-time PCR in the 3 types of specimens were compared to those by ProbeTec ET assay (ProbeTec, BD Diagnostic System). The overall positive concordance between real-time PCR and ProbTec were 97.1% (66/68) for C. trachomatis and 100% (33/33) for N. gonorrhoeae, C. trachomatis detection yielded 3 discordant results in endocervical specimens and 1 discordant result in male FVU by real-time PCR and ProbTec. Three of 4 reexamined using Aptime Combo 2 Assay (Fuji Rebio Inc.) were positive for C. trachomatis. Endocervical swab and FVU specimen results for C. trachomatis were discordant in 3 cases in real-time PCR and 4 in ProbeTec. Subjects with 2 or more positive endocervical awab results in female or male FVU specimens were assumed to be "true positive" for C. trachomatis. The sensitivities of real-time PCR for detecting C. trachomatis was 94.4% in endocervical swabs, 77.8% in female FVU and 97.4% in the male FVU. The sensitivities for real-time PCR for detecting N. gonorrhoeae was 100% in all 3 specimentypes. Abbott RealTime CT/NG assay was useful for detecting C. trachomatis using endocervical swabs or male FVU specimens and for detecting N. gonorrhoeae using endocervical swabs and all FVU specimens.
authors.affiliation: Department of Urology, University of Occupational and Environmental Health.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Hamasuna
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kawai
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ando
authors.forename: Kenji
authors.initials: K
authors.lastname: Ito
authors.forename: Motoko
authors.initials: M
authors.lastname: Kurashima
authors.forename: Hirohumi
authors.initials: H
authors.lastname: Nishimura
authors.forename: Takamasa
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Muratani
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D001431:Bacteriological Techniques; D002584:Cervix Uteri / Q000382:microbiology*; D002690:Chlamydia Infections / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D006069:Gonorrhea / Q000382:microbiology; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014526:Urethritis / Q000382:microbiology; D014556:Urine / Q000382:microbiology*; D002575:Uterine Cervicitis / Q000382:microbiology