title: Somatic cells derived from haploid larvae are feasible as donors for nuclear transplant in zebrafish. Preliminary results.
abstract: Summary            Somatic cells derived from zebrafish haploid larval (both androgenetic and gynogenetic) cultures were used as donors for nuclear transplant into non-enucleated oocytes. Nuclei were transplanted either before or simultaneously with oocyte activation in the central region and in the incipient animal pole, respectively. Against expected results, 20% of transplanted embryos during oocyte activation using cells of gynogenetic origin reached the 100% epiboly stage, even two survived for up to 5 days, whereas no development was observed when cells from androgenetic origin were used. Results derived from this work open a novel possibility of studying somatic cell reprogramming and imprinting phenomena in zebrafish.
authors.affiliation: Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14, 46022 Valencia, Spain. cardona_costa_j@hotmail.com
authors.forename: J
authors.initials: J
authors.lastname: Cardona-Costa
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Camps
authors.forename: F
authors.initials: F
authors.lastname: García-Ximénez
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D019976:Cloning, Organism; D006238:Haploidy; D007814:Larva / Q000254:growth & development*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000378:metabolism*