title: [Culture and identification of neural stem cells from mouse embryos].
abstract: OBJECTIVE
  The purpose of this study was to culture and identify neural stem cells from mouse embryos in vitro using a modified method and provide a basis for further study of the biology of neural stem cells under hypoxia.
  
  
  METHODS
  The cells were isolated mechanically from the front cortex of fetal Institute of Cancer Research (ICR) mice on embryonic day 14. They were passaged by mechanical dissociation and enzymatic digestion. The neurospheres were identified by immunofluorescent staining of nestin. Cell differentiation was induced by 1% fetal bovine serum and then the cells were identified by immunohistochemistry of β-tubulin III and GFAP.
  
  
  RESULTS
  The cells obtained from the front cortex of fetal ICR mice had the capacity of forming neurospheres which showed nestin immunoreactive positivity. After being induced by 1% fetal bovine serum, the cells were differentiated into β-tubulin III-positive cells and GFAP-positive cells.
  
  
  CONCLUSIONS
  Using mechanical dissociation of primary cells and mechanical dissociation with enzymatic digestion of primary cells, the NSCs from the front cortex of mouse embryos can be obtained.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710004, China. zhpbo@163.com
authors.forename: Peng-Bo
authors.initials: PB
authors.lastname: Zhang
authors.forename: Wei-Song
authors.initials: WS
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Ni
authors.initials: N
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Lei
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Lv
authors.forename: Xin-Lin
authors.initials: XL
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007381:Intermediate Filament Proteins / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000032:analysis; D064231:Nestin; D058953:Neural Stem Cells / Q000737:chemistry / Q000166:cytology*; D014404:Tubulin / Q000032:analysis
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D014404:Tubulin