title: Surface modification and biomolecule immobilization on polymer spheres for biosensing applications.
abstract: Microspheres and nanospheres are being used in many of today's biosensing applications for automated sample processing, flow cytometry, signal amplification in microarrays, and labeling in multiplexed analyses. The surfaces of the spheres/particles need to be modified with proteins and other biomolecules to be used in these sensing applications. This chapter contains protocols to modify carboxyl- and amine-coated polymer spheres with proteins and peptides.
authors.affiliation: Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC, USA.
authors.forename: Chris R
authors.initials: CR
authors.lastname: Taitt
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Shriver-Lake
authors.forename: George P
authors.initials: GP
authors.lastname: Anderson
authors.forename: Frances S
authors.initials: FS
authors.lastname: Ligler
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D037341:Fumonisins / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D008863:Microspheres; D036103:Nanotechnology; D009793:Ochratoxins / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D013499:Surface Properties
chemical_list: D023181:Antimicrobial Cationic Peptides; D037341:Fumonisins; D009793:Ochratoxins; D011108:Polymers; D011506:Proteins; C025589:ochratoxin A; C056933:fumonisin B1