title: The relationship between redox enzyme activity and electrochemical potential-cellular and mechanistic implications from protein film electrochemistry.
abstract: In protein film electrochemistry a redox protein of interest is studied as an electroactive film adsorbed on an electrode surface. For redox enzymes this configuration allows quantification of the relationship between catalytic activity and electrochemical potential. Considered as a function of enzyme environment, i.e., pH, substrate concentration etc., the activity-potential relationship provides a fingerprint of activity unique to a given enzyme. Here we consider the nature of the activity-potential relationship in terms of both its cellular impact and its origin in the structure and catalytic mechanism of the enzyme. We propose that the activity-potential relationship of a redox enzyme is tuned to facilitate cellular function and highlight opportunities to test this hypothesis through computational, structural, biochemical and cellular studies.
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Chemistry and School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Gates
authors.forename: Gemma L
authors.initials: GL
authors.lastname: Kemp
authors.forename: Chun Yip
authors.initials: CY
authors.lastname: To
authors.forename: James
authors.initials: J
authors.lastname: Mann
authors.forename: Sophie J
authors.initials: SJ
authors.lastname: Marritt
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Mayes
authors.forename: David J
authors.initials: DJ
authors.lastname: Richardson
authors.forename: Julea N
authors.initials: JN
authors.lastname: Butt
mesh_terms: D055162:Biocatalysis; D055664:Electrochemical Techniques / Q000379:methods; D004563:Electrochemistry; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D050901:Nitrate Reductase / Q000737:chemistry* / Q000378:metabolism; D009566:Nitrates / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D042084:Paracoccus pantotrophus / Q000201:enzymology; D013329:Structure-Activity Relationship
chemical_list: D004798:Enzymes; D009566:Nitrates; D050901:Nitrate Reductase