title: Rapid cellular turnover in adipose tissue.
abstract: It was recently shown that cellular turnover occurs within the human adipocyte population. Through three independent experimental approaches--dilution of an inducible histone 2B-green fluorescent protein (H2BGFP), labeling with the cell cycle marker Ki67 and incorporation of BrdU--we characterized the degree of cellular turnover in murine adipose tissue. We observed rapid turnover of the adipocyte population, finding that 4.8% of preadipocytes are replicating at any time and that between 1-5% of adipocytes are replaced each day. In light of these findings, we suggest that adipose tissue turnover represents a possible new avenue of therapeutic intervention against obesity.
authors.affiliation: Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Boston, Massachusetts, United States of America.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Rigamonti
authors.forename: Kristen
authors.initials: K
authors.lastname: Brennand
authors.forename: Frank
authors.initials: F
authors.lastname: Lau
authors.forename: Chad A
authors.initials: CA
authors.lastname: Cowan
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle; D049109:Cell Proliferation; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D009765:Obesity / Q000473:pathology
chemical_list: D019394:Ki-67 Antigen; D001973:Bromodeoxyuridine