title: Tracking the activation of Stat5 through the expression of an inducible reporter gene in a transgenic mouse line.
abstract: Signal transducer and activator of transcription 5 (Stat5), a latent cytoplasmic transcription factor, becomes activated by phosphorylation upon cytokine, hormone, and growth factor interactions with their appropriate receptors and induces the transcription of target genes. It plays crucial roles in principal cell fate decisions and regulates cell differentiation, development, proliferation, apoptosis, and inflammation. It is active in the mammary gland, the liver, hematopoietic cells, and other organs and has pleiotropic functions, depending on its activation pathway and its site of action. We derived transgenic mice in which the expression of a LacZ reporter gene is directed by Stat5-specific response elements and visualized the activation of Stat5 in cells of mouse organs at different developmental stages. The reporter gene activity reflects the timing and the location of Stat5 activation and was documented in mammary epithelial cells during developmental stages of the gland, cells of the liver, kidney, spleen, thymus, and uterus and in granulocytes and macrophages of the transgenic lines.
authors.affiliation: Georg-Speyer-Haus, Institute for Biomedical Research, D-60596 Frankfurt/Main, Germany. groner@em.uni-frankfurt.de
authors.forename: Nadja Lydia
authors.initials: NL
authors.lastname: Bednorz
authors.forename: Boris
authors.initials: B
authors.lastname: Brill
authors.forename: Andreas
authors.initials: A
authors.lastname: Klein
authors.forename: Katrin
authors.initials: K
authors.lastname: Gäbel
authors.forename: Bernd
authors.initials: B
authors.lastname: Groner
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter / Q000235:genetics*; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D007763:Lac Operon / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011247:Pregnancy; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D050799:STAT5 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D050799:STAT5 Transcription Factor; D001616:beta-Galactosidase