title: Molecular basis of macrolide, triamilide, and lincosamide resistance in Pasteurella multocida from bovine respiratory disease.
abstract: The mechanism of macrolide-triamilide resistance in Pasteurella multocida has been unknown. During whole-genome sequencing of a multiresistant bovine P. multocida isolate, three new resistance genes, the rRNA methylase gene erm(42), the macrolide transporter gene msr(E), and the macrolide phosphotransferase gene mph(E), were detected. The three genes were PCR amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triamilide resistance [msr(E)-mph(E)] in macrolide-susceptible Escherichia coli and P. multocida hosts.
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Höltystr. 10, 31535 Neustadt-Mariensee, Germany.
authors.forename: Kristina
authors.initials: K
authors.lastname: Kadlec
authors.forename: Geovana
authors.initials: G
authors.lastname: Brenner Michael
authors.forename: Michael T
authors.initials: MT
authors.lastname: Sweeney
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Brzuszkiewicz
authors.forename: Heiko
authors.initials: H
authors.lastname: Liesegang
authors.forename: Rolf
authors.initials: R
authors.lastname: Daniel
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Watts
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002417:Cattle; D055231:Lincosamides / Q000494:pharmacology*; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016979:Pasteurella multocida / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D012140:Respiratory Tract Diseases / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D055231:Lincosamides; D018942:Macrolides