title: Previous chronic exogenous glucocorticoid administration in vivo does not affect functional characteristics and cellular lifespan of human skin fibroblasts in vitro.
abstract: Excess of glucocorticoids (GCs) has been reported to lead to skin atrophy and impaired wound healing. The present study investigates whether human skin fibroblasts suffer permanent damages due to a long-term exposure to GC excess. Fibroblasts obtained from patients being under GC treatment for periods over one year were cultured under standard conditions in vitro, and studied regarding pivotal parameters involved in skin homeostasis and aging, i.e. collagen production, cell proliferation, and cellular replicative lifespan. No statistical differences were observed regarding these functions compared to those of normal human skin fibroblasts. Furthermore, no differences between normal and patient-derived cells were observed regarding their sensitivity to a supra-physiological cortisol concentration. In conclusion, the prolonged exposure of human skin fibroblasts in vivo to high concentrations of exogenously-administered GC does not lead to persistent adverse effects on their physiology.
authors.forename: Harris
authors.initials: H
authors.lastname: Pratsinis
authors.forename: Anastasia
authors.initials: A
authors.lastname: Dimozi
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Pilichos
authors.forename: Stylianos
authors.initials: S
authors.lastname: Tsagarakis
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Yiacoumettis
authors.forename: Dimitris
authors.initials: D
authors.lastname: Kletsas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D016922:Cellular Senescence / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005938:Glucocorticoids / Q000008:administration & dosage / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D005938:Glucocorticoids; D012333:RNA, Messenger; D003094:Collagen