title: Techniques for cultivating methanogens.
abstract: Basic techniques for the cultivation of methanogenic archaea in anoxic media, where the O/R potential is maintained below (-) 330 mV under a pressurized atmosphere of 20% carbon dioxide, are described.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana, Illinois, USA.
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Wolfe
mesh_terms: D001105:Archaea / Q000254:growth & development* / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D008697:Methane / Q000096:biosynthesis*
chemical_list: D002245:Carbon Dioxide; D008697:Methane