title: YB-1 evokes susceptibility to cancer through cytokinesis failure, mitotic dysfunction and HER2 amplification.
abstract: Y-box binding protein-1 (YB-1) expression in the mammary gland promotes breast carcinoma that demonstrates a high degree of genomic instability. In the present study, we developed a model of pre-malignancy to characterize the role of this gene during breast cancer initiation and early progression. Antibody microarray technology was used to ascertain global changes in signal transduction following the conditional expression of YB-1 in human mammary epithelial cells (HMEC). Cell cycle-associated proteins were frequently altered with the most dramatic being LIM kinase 1/2 (LIMK1/2). Consequently, the misexpression of LIMK1/2 was associated with cytokinesis failure that acted as a precursor to centrosome amplification. Detailed investigation revealed that YB-1 localized to the centrosome in a phosphorylation-dependent manner, where it complexed with pericentrin and γ-tubulin. This was found to be essential in maintaining the structural integrity and microtubule nucleation capacity of the organelle. Prolonged exposure to YB-1 led to rampant acceleration toward tumorigenesis, with the majority of cells acquiring numerical and structural chromosomal abnormalities. Slippage through the G(1)/S checkpoint due to overexpression of cyclin E promoted continued proliferation of these genomically compromised cells. As malignancy further progressed, we identified a subset of cells harboring HER2 amplification. Our results recognize YB-1 as a cancer susceptibility gene, with the capacity to prime cells for tumorigenesis.
authors.affiliation: Laboratory of Oncogenomic Research, Departments of Pediatrics and Experimental Medicine, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: A H
authors.initials: AH
authors.lastname: Davies
authors.forename: I
authors.initials: I
authors.lastname: Barrett
authors.forename: M R
authors.initials: MR
authors.lastname: Pambid
authors.forename: K
authors.initials: K
authors.lastname: Hu
authors.forename: A L
authors.initials: AL
authors.lastname: Stratford
authors.forename: S
authors.initials: S
authors.lastname: Freeman
authors.forename: I M
authors.initials: IM
authors.lastname: Berquin
authors.forename: S
authors.initials: S
authors.lastname: Pelech
authors.forename: P
authors.initials: P
authors.lastname: Hieter
authors.forename: C
authors.initials: C
authors.lastname: Maxwell
authors.forename: S E
authors.initials: SE
authors.lastname: Dunn
mesh_terms: D000782:Aneuploidy; D002453:Cell Cycle; D004198:Disease Susceptibility; D018734:Genes, erbB-2; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008938:Mitosis; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D051840:Y-Box-Binding Protein 1 / Q000502:physiology*
chemical_list: D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human