title: Species differences of macrophage very low-density-lipoprotein (VLDL) receptor protein expression.
abstract: Triglyceride-rich lipoproteins (TGRLs) and low-density-lipoprotein (LDL) cholesterol are independent risk factors for coronary artery disease. We have previously proposed that the very low-density-lipoprotein (VLDL) receptor is one of the receptors required for foam cell formation by TGRLs in human macrophages. However, the VLDL receptor proteins have not been detected in atherosclerotic lesions of several animal models. Here we showed no VLDL receptor protein was detected in mouse macrophage cell lines (Raw264.7 and J774.2) or in mouse peritoneal macrophages in vitro. Furthermore, no VLDL receptor protein was detected in macrophages in atherosclerotic lesions of chow-fed apolipoprotein E-deficient or cholesterol-fed LDL receptor-deficient mice in vivo. In contrast, macrophage VLDL receptor protein was clearly detected in human macrophages in vitro and in atherosclerotic lesions in myocardial infarction-prone Watanabe-heritable hyperlipidemic (WHHLMI) rabbits in vivo. There are species differences in the localization of VLDL receptor protein in vitro and in vivo. Since VLDL receptor is expressed on macrophages in atheromatous plaques of both rabbit and human but not in mouse models, the mechanisms of atherogenesis and/or growth of atherosclerotic lesions in mouse models may be partly different from those of humans and rabbits.
authors.affiliation: Third Department of Internal Medicine, University of Fukui, Faculty of Medical Science, Japan. sadaost@u-fukui.ac.jp
authors.forename: Sadao
authors.initials: S
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Zenimaru
authors.forename: Jinya
authors.initials: J
authors.lastname: Suzuki
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyamori
authors.forename: Masao
authors.initials: M
authors.lastname: Takahashi
authors.forename: Masafumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ishida
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Ishida
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Hirata
authors.forename: Tokuo T
authors.initials: TT
authors.lastname: Yamamoto
authors.forename: Tadao
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hattori
authors.forename: Masashi
authors.initials: M
authors.lastname: Shiomi
mesh_terms: D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D011817:Rabbits; D011973:Receptors, LDL / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D011973:Receptors, LDL; C036053:VLDL receptor