title: Joint inflammation and early degeneration induced by high-force reaching are attenuated by ibuprofen in an animal model of work-related musculoskeletal disorder.
abstract: We used our voluntary rat model of reaching and grasping to study the effect of performing a high-repetition and high-force (HRHF) task for 12 weeks on wrist joints. We also studied the effectiveness of ibuprofen, administered in the last 8 weeks, in attenuating HRHF-induced changes in these joints. With HRHF task performance, ED1+ and COX2+ cells were present in subchondral radius, carpal bones and synovium; IL-1alpha and TNF-alpha increased in distal radius/ulna/carpal bones; chondrocytes stained with Terminal deoxynucleotidyl Transferase- (TDT-) mediated dUTP-biotin nick end-labeling (TUNEL) increased in wrist articular cartilages; superficial structural changes (e.g., pannus) and reduced proteoglycan staining were observed in wrist articular cartilages. These changes were not present in normal controls or ibuprofen treated rats, although IL-1alpha was increased in reach limbs of trained controls. HRHF-induced increases in serum C1,2C (a biomarker of collagen I and II degradation), and the ratio of collagen degradation to synthesis (C1,2C/CPII; the latter a biomarker of collage type II synthesis) were also attenuated by ibuprofen. Thus, ibuprofen treatment was effective in attenuating HRHF-induced inflammation and early articular cartilage degeneration.
authors.affiliation: Division of Rheumatology, Tufts Medical Center, 800 Washington Street, Box 406, Boston, MA 02111, USA.
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Driban
authors.forename: Ann E
authors.initials: AE
authors.lastname: Barr
authors.forename: Mamta
authors.initials: M
authors.lastname: Amin
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sitler
authors.forename: Mary F
authors.initials: MF
authors.lastname: Barbe
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000473:pathology; D002357:Cartilage Diseases / Q000188:drug therapy* / Q000473:pathology; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology; D019902:Chondrocytes / Q000378:metabolism; D003094:Collagen / Q000097:blood; D005260:Female; D007052:Ibuprofen / Q000008:administration & dosage / Q000627:therapeutic use*; D010446:Peptide Fragments / Q000097:blood; D011347:Procollagen / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D016474:Weight-Bearing; D014955:Wrist Joint / Q000187:drug effects* / Q000473:pathology
chemical_list: D010446:Peptide Fragments; D011347:Procollagen; D014409:Tumor Necrosis Factor-alpha; C093212:procollagen type II carboxy-terminal peptide; D003094:Collagen; D007052:Ibuprofen