title: Phenolic composition and antioxidant properties of poplar bud (Populus nigra) extract: individual antioxidant contribution of phenolics and transcriptional effect on skin aging.
abstract: The Populus species possess great potential for therapeutical applications, especially for their known anti-inflammatory properties. The antioxidant properties of propolis, a hive product collected by honey bees mainly from poplar bud exudates, suggest that poplar buds also possess antioxidant properties. Here is reported the characterization of the antioxidant properties of an aqueous poplar bud (Populus nigra) extract. It presented a high total phenolic content, and moderate antioxidant properties as determined by ORAC assay. The main phenolic compounds identified were phenolic acids and flavonoid aglycons. These phenolic compounds were analyzed by ORAC assay for their individual antioxidant activity, in order to determine the major contributors to the total antioxidant activity of the extract. Thanks to their high antioxidant activity, caffeic and p-coumaric acids were identified as the major antioxidant components. Representing only 3.5% of its dry weight, these compounds represented together about 50% of the total antioxidant activity of the extract. The antioxidant properties of poplar bud extract and the phenolic compounds identified were also analyzed by cellular antioxidant activity assay (CAA), which was weakly correlated with ORAC assay. The transcriptional effect of poplar bud extract on skin aging was evaluated in vitro on a replicative senescence model of normal human dermal fibroblasts, using a customized DNA macroarray specifically designed to investigate skin aging markers. Among the detected genes, poplar bud extract significantly regulated genes involved in antioxidant defenses, inflammatory response and cell renewal. The collective antioxidant properties and transcriptional effect of this extract suggest potential antiaging properties which could be utilized in cosmetic and nutraceutical formulations.
authors.affiliation: Biolandes Route de Bélis, Le Sen, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Dudonné
authors.forename: Pascal
authors.initials: P
authors.lastname: Poupard
authors.forename: Philippe
authors.initials: P
authors.lastname: Coutière
authors.forename: Marion
authors.initials: M
authors.lastname: Woillez
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Xavier
authors.initials: X
authors.lastname: Vitrac
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D032107:Populus / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects* / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts