title: Effect of trans-fat, fructose and monosodium glutamate feeding on feline weight gain, adiposity, insulin sensitivity, adipokine and lipid profile.
abstract: The incidence of obesity and type 2 diabetes mellitus (T2DM) is increasing, and new experimental models are required to investigate the diverse aspects of these polygenic diseases, which are intimately linked in terms of aetiology. Feline T2DM has been shown to closely resemble human T2DM in terms of its clinical, pathological and physiological features. Our aim was to develop a feline model of diet-induced weight gain, adiposity and metabolic deregulation, and to examine correlates of weight and body fat change, insulin homeostasis, lipid profile, adipokines and clinical chemistry, in order to study associations which may shed light on the mechanism of diet-induced metabolic dysregulation. We used a combination of partially hydrogenated vegetable shortening and high-fructose corn syrup to generate a high-fat-high-fructose diet. The effects of this diet were compared with an isoenergetic standard chow, either in the presence or absence of 1.125 % dietary monosodium glutamate (MSG). Dual-energy X-ray absorptiometry body imaging and a glucose tolerance test were performed. The present results indicate that dietary MSG increased weight gain and adiposity, and reduced insulin sensitivity (P < 0.05), whereas high-fat-high-fructose feeding resulted in elevated cortisol and markers of liver dysfunction (P < 0.01). The combination of all three dietary constituents resulted in lower insulin levels and elevated serum β-hydroxybutyrate and cortisol (P < 0.05). This combination also resulted in a lower first-phase insulin release during glucose tolerance testing (P < 0.001). In conclusion, markers of insulin deregulation and metabolic dysfunction associated with adiposity and T2DM can be induced by dietary factors in a feline model.
authors.affiliation: Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. kate@kfshrc.edu.sa
authors.forename: Kate S
authors.initials: KS
authors.lastname: Collison
authors.forename: Marya Z
authors.initials: MZ
authors.lastname: Zaidi
authors.forename: Soad M
authors.initials: SM
authors.lastname: Saleh
authors.forename: Angela
authors.initials: A
authors.lastname: Inglis
authors.forename: Rhea
authors.initials: R
authors.lastname: Mondreal
authors.forename: Nadine J
authors.initials: NJ
authors.lastname: Makhoul
authors.forename: Razan
authors.initials: R
authors.lastname: Bakheet
authors.forename: Joey
authors.initials: J
authors.lastname: Burrows
authors.forename: Norton W
authors.initials: NW
authors.lastname: Milgram
authors.forename: Futwan A
authors.initials: FA
authors.lastname: Al-Mohanna
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D015502:Absorptiometry, Photon; D054392:Adipokines / Q000097:blood; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D050154:Adiposity / Q000187:drug effects; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002415:Cats; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000378:metabolism / Q000662:veterinary; D004032:Diet; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000009:adverse effects; D019422:Dietary Sucrose / Q000009:adverse effects; D004195:Disease Models, Animal; D005632:Fructose / Q000009:adverse effects*; D005951:Glucose Tolerance Test; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects; D009765:Obesity / Q000209:etiology* / Q000378:metabolism / Q000662:veterinary; D010938:Plant Oils / Q000009:adverse effects; D012970:Sodium Glutamate / Q000009:adverse effects*; D044242:Trans Fatty Acids / Q000009:adverse effects*; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D054392:Adipokines; D015415:Biomarkers; D004041:Dietary Fats; D019422:Dietary Sucrose; D007328:Insulin; D008055:Lipids; D010938:Plant Oils; D044242:Trans Fatty Acids; D005632:Fructose; D020155:3-Hydroxybutyric Acid; D012970:Sodium Glutamate; D006854:Hydrocortisone