title: Sandblasted-acid-etched titanium surface influences in vitro the biological behavior of SaOS-2 human osteoblast-like cells.
abstract: Osseointegrated dental implants have been successfully used over the past several years, allowing functional replacement of missing teeth. Surface properties of titanium dental implants influence bone cell response. Implant topography appears to modulate cell growth and differentiation of osteoblasts thus affecting the bone healing process. Optimal roughness and superficial morphology are still controversial and need to be clearly defined. In the present study we evaluated in vitro the biological behavior of SaOS-2 cells, a human osteoblast-like cell line, cultured on two different titanium surfaces, smooth and sandblasted-acid-etched, by investigating cell morphology, adhesion, proliferation, expression of some bone differentiation markers and extracellular matrix components. Results showed that the surface topography may influence in vitro the phenotypical expression of human osteoblast-like cells. In particular the tested sandblasted-acid-etched titanium surface induced a significantly increased Co I deposition and α2-β1 receptor expression as compared to the relatively smooth surface, promoting a probable tendency of SaOS-2 cells to shift toward a mature osteoblastic phenotype. It is therefore likely that specific surface properties of sandblasted-acid-etched titanium implants may modulate the biological behavior of osteoblasts during bone tissue healing.
authors.affiliation: Department of Oral and Maxillo-Facial Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy. luca.ramaglia@unina.it
authors.forename: Luca
authors.initials: L
authors.lastname: Ramaglia
authors.forename: Loredana
authors.initials: L
authors.lastname: Postiglione
authors.forename: Gaetano
authors.initials: G
authors.lastname: Di Spigna
authors.forename: Gabriele
authors.initials: G
authors.lastname: Capece
authors.forename: Salvatore
authors.initials: S
authors.lastname: Salzano
authors.forename: Guido
authors.initials: G
authors.lastname: Rossi
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D000469:Alkaline Phosphatase / Q000032:analysis; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D024042:Collagen Type I / Q000032:analysis; D045662:Dental Etching / Q000379:methods*; D003764:Dental Materials / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D039421:Integrin alpha2 / Q000032:analysis; D039482:Integrin alpha5 / Q000032:analysis; D039503:Integrin alpha6 / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D013464:Sulfuric Acids / Q000737:chemistry; D013499:Surface Properties; D019063:Tenascin / Q000032:analysis; D014025:Titanium / Q000737:chemistry*; D014945:Wound Healing / Q000502:physiology
chemical_list: D024042:Collagen Type I; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039421:Integrin alpha2; D039482:Integrin alpha5; D039503:Integrin alpha6; D019012:Integrin beta1; D015850:Interleukin-6; D013464:Sulfuric Acids; D019063:Tenascin; D014025:Titanium; D000469:Alkaline Phosphatase; C033158:sulfuric acid; D006851:Hydrochloric Acid