title: HPLC-MS aided PC12 cell systems: to quantitatively monitor the conversion of nitronyl nitroxide in biological systems with and without NO.
abstract: Nitronyl nitroxides are capable of preventing cells, tissues, and organs from radical-induced damage through scavenging NO˙, ˙O(2)(-) and ˙OH. In order to explore the conversions of nitronyl nitroxides in biological systems with and without NO˙, HPLC-MS aided PC12 cell systems were developed, and the conversions of 2-(3'-nitrophenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl -3-oxide (3-nitro-PTIO), 1-oxyl-2-(3'-nitrophenyl)-4,4,5,5-tetramethylimidazoline (3-nitro-PTI), and 1-hydroxyl-2-(3'-nitrophenyl)-4,4,5,5-tetramethylimidazoline (3-nitro-PTIH) were quantitatively monitored. In these systems 3-nitro-PTIO and 3-nitro-PTI were time-dependently converted to 3-nitro-PTIH, while no conversion of 3-nitro-PTIH was detected. Free radical NO˙ donors (sodium nitroprusside, SNP) accelerated the conversions, but had no effect upon the conversion product. In the in vitro and in vivo assays the 3-nitro-PTIH treated cells and mice exhibited no toxic response.
authors.affiliation: College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Wang
authors.forename: Xueyun
authors.initials: X
authors.lastname: Jiang
authors.forename: Jiawang
authors.initials: J
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Guifeng
authors.initials: G
authors.lastname: Kang
authors.forename: Jianhui
authors.initials: J
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.forename: Shiqi
authors.initials: S
authors.lastname: Peng
mesh_terms: D000818:Animals; D001669:Biochemical Phenomena / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000378:metabolism; D005260:Female; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D015394:Molecular Structure; D009569:Nitric Oxide / Q000737:chemistry* / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000378:metabolism / Q000494:pharmacology; D009589:Nitrogen Oxides / Q000737:chemistry* / Q000378:metabolism; D009599:Nitroprusside / Q000378:metabolism / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors
chemical_list: D003497:Cyclic N-Oxides; D007093:Imidazoles; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D009599:Nitroprusside; C079391:2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide; D009569:Nitric Oxide; C039900:nitroxyl