title: HMGA Interactome: new insights from phage display technology.
abstract: High mobility group A proteins (HMGA1 and HMGA2) are architectural factors involved in chromatin remodelling and regulation of gene expression. HMGA are highly expressed during embryogenesis and in cancer cells and are involved in development and cell differentiation as well as cancer formation and progression. These factors, by binding to DNA and interacting with other nuclear proteins, can organize macromolecular complexes involved in transcription, chromatin dynamics, RNA processing, and DNA repair. The identification of protein partners for HMGA has greatly contributed to our understanding of their multiple functions. He we report the identification of HMGA molecular partners using a gene fragment library in a phage display screening. Using an ORF-enriched cDNA library, we have isolated several HMGA1 interacting clones and for two of them, TBP associated factor 3 (TAF3) and chromatin assembly factor 1 p150/CAF-1, have demonstrated an in vivo association with HMGA1. The identification of these new partners suggests that HMGA can also influence general aspects of transcription and once more underlines their involvement in chromatin remodelling and dynamics.
authors.affiliation: Department of Life Sciences, University of Trieste, Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Malini
authors.forename: Elisa
authors.initials: E
authors.lastname: Maurizio
authors.forename: Sara
authors.initials: S
authors.lastname: Bembich
authors.forename: Riccardo
authors.initials: R
authors.lastname: Sgarra
authors.forename: Paolo
authors.initials: P
authors.lastname: Edomi
authors.forename: Guidalberto
authors.initials: G
authors.lastname: Manfioletti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D056504:Chromatin Assembly Factor-1 / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D057809:HEK293 Cells; D006609:High Mobility Group Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D019151:Peptide Library; D025941:Protein Interaction Mapping; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017384:Sequence Deletion; D035381:TATA-Binding Protein Associated Factors / Q000737:chemistry
chemical_list: D056504:Chromatin Assembly Factor-1; D018076:DNA, Complementary; D006609:High Mobility Group Proteins; D019151:Peptide Library; D011994:Recombinant Proteins; D035381:TATA-Binding Protein Associated Factors