title: Development and validation of a liquid chromatography method with electrospray ionization tandem mass spectrometry for the determination of brotizolam residues in beef and commercial whole milk.
abstract: In this work, a liquid chromatography-tandem mass spectrometric detection technique was developed and validated for the determination of brotizolam residues in beef muscle and commercial whole milk. This procedure involves the extraction of the analyte from the samples via liquid-solid extraction, and caffeine was used as an internal standard. The analyte was successfully separated on an XTerra-C(18) column, with a mobile phase composed of 0.01% formic acid in acetonitrile and 1 mm ammonium formate-0.01% formic acid in water. The one-step extraction method evidenced good selectivity, precision (RSD = 9.87-26.47%), and the recovery of the extractable analyte was 92.61-115.98% in the matrices. The limits of quantification ranged between 0.4 and 0.5 µg/kg. The developed method is simple since it requires no additional cleanup procedures.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Cho
authors.forename: Kyul
authors.initials: K
authors.lastname: Jo
authors.forename: Hee
authors.initials: H
authors.lastname: Yi
authors.forename: Soo Min
authors.initials: SM
authors.lastname: Cho
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: Min Kyung
authors.initials: MK
authors.lastname: Kim
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Han
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Oh
authors.forename: Chan Hyeok
authors.initials: CH
authors.lastname: Kwon
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin
mesh_terms: D000818:Animals; D001381:Azepines / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D001381:Azepines; C020960:brotizolam