title: Comparison of the biological response of osteoblasts after tension and compression.
abstract: The aim of this study was to investigate the difference in the biological response of osteoblasts when stretched and compressed. A cellular cyclic tension and compression apparatus (CCTCA) was designed to stretch and compress cells under the same conditions. After stretching or compressing MC3T3-E1 with continuously increased strain for 5 hours, cellular cytoskeletal modulation was detected by immunohistochemical assay with actin antibody. Real-time polymerase chain reaction was performed at 1, 3, and 5 hours to detect local factors related to bone remodelling. Statistical analysis was undertaken with analysis of variance and the Kruskal-Wallis.  Following stretching or compression for 5 hours, MC3T3-E1 attached to the culture dishes grew well. Compared with the control, the microfilaments orientated parallel with each other and were clearly observed by laser scanning confocal microscope after 5 hours of stretching. The morphology of MC3T3-E1 cells was thinner and longer than the control. However, microfilaments presented a disordered arrangement after 5 hours of compression, and the MC3T3-E1 cells decreased in size. Gene expression of Wnt10b and Lrp5 increased during tension but more in the compression groups at 1, 3, and 5 hours. The ratio of osteoprotegerin to receptor activator for nuclear factor kappa B ligand increased in the tension group compared with the control but decreased in the compression group at 5 hours.
authors.affiliation: Department of Orthodontics, School and Hospital of Stomatology, Peking University, Beijing, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhong
authors.forename: Xiang-Long
authors.initials: XL
authors.lastname: Zeng
authors.forename: Ju-Hua
authors.initials: JH
authors.lastname: Ni
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Huang
mesh_terms: D016475:3T3 Cells; D008841:Actin Cytoskeleton; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D019245:Compressive Strength; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression; D008074:Lipoproteins / Q000378:metabolism; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D053244:Osteoprotegerin / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013718:Tensile Strength; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D000199:Actins; D008074:Lipoproteins; D053244:Osteoprotegerin; D011518:Proto-Oncogene Proteins; D053245:RANK Ligand; C491119:WNT10B protein, human; D051153:Wnt Proteins