title: A novel transfection method for eukaryotic cells using polyethylenimine coated albumin microbubbles.
abstract: Albumin microbubbles have been intensively studied for their application in gene delivery. However, with negative surface potential, albumin microbubbles hardly bind plasmid DNA, which might contribute to their low transgene efficiency. In this study, we developed polyethylenimine (PEI) coated albumin microbubbles (PAMB) which were prepared by sonicating the mixture of human albumin, PEI, polyethylene glycol and glucose. CHO cells, COS cells and 293T cells were transfected with PEI, PEI+albumin, PAMB and Lipofectamine 2000, respectively. Our results showed that the surface potential was elevated and PAMB could bind plasmid DNA. The transgene efficiency of PAMB was higher than PEI and PEI+albumin (P<0.05), and PAMB performed the same transgene effect as Lipofectamine 2000 did but with lower cytotoxicity than Lipofectamine 2000. Albumin microbubbles modified by PEI has high transgene efficiency and low cytotoxicity even without ultrasound medication, making it a useful non-virus gene delivery method in vitro.
authors.affiliation: Institute of Medical Biotechnology, School of Medicine, Soochow University, Suzhou 215007, China.
authors.forename: Shi-peng
authors.initials: SP
authors.lastname: Dang
authors.forename: Ru-xing
authors.initials: RX
authors.lastname: Wang
authors.forename: Ming-de
authors.initials: MD
authors.lastname: Qin
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan-zheng
authors.initials: YZ
authors.lastname: Gu
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Wang
authors.forename: Qiao-lin
authors.initials: QL
authors.lastname: Yang
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Li
authors.forename: Xue-guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000008:administration & dosage*; D005466:Fluorocarbons; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000633:toxicity; D045423:Microbubbles; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D013010:Sonication; D014162:Transfection / Q000379:methods*; D019076:Transgenes
chemical_list: D000418:Albumins; D005466:Fluorocarbons; D008055:Lipids; C086724:Lipofectamine; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA; C042852:perflutren