title: A quantitative method for simultaneous determination of 5-methoxy-N,N-diisopropyltryptamine and its metabolites in urine using liquid chromatography-electrospray ionization-tandem mass spectrometry.
abstract: 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) is a designer hallucinogen derived from tryptamine and is reportedly abused and involved in criminal activities. For the detection of 5-MeO-DIPT use, a liquid chromatography-tandem mass spectrometric method for 5-MeO-DIPT and its metabolites, 5-hydroxy-N,N-diisopropyltryptamine (5-OH-DIPT) and 5-methoxy-N,N-isopropyltryptamine (5-MeO-IPT) was developed and validated in rat urine. The urine samples were pretreated by protein precipitation with acetonitrile and introduced into a BDS HYPERSIL C(18) column (50 × 2.0 mm, 5 μm) for chromatographic separation. Mobile phases consisted of methanol, water, and 1% formic acid, and gradient elution was used at a flow rate of 0.2 mL/min. For the MS detection, multiple-reaction monitoring analysis was adopted. The linear range was 0.01-10 μg/mL, and the lower limit of quantification was 10 ng/mL for all analytes. The intra- and interday accuracies and precisions met the criteria (<15%). The developed method was successfully applied to the drug-treated rat urine.
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul 130-650, South Korea.
authors.forename: Ming J
authors.initials: MJ
authors.lastname: Jin
authors.forename: Changbae
authors.initials: C
authors.lastname: Jin
authors.forename: Jin Y
authors.initials: JY
authors.lastname: Kim
authors.forename: Moon K
authors.initials: MK
authors.lastname: In
authors.forename: Oh S
authors.initials: OS
authors.lastname: Kwon
authors.forename: Hye H
authors.initials: HH
authors.lastname: Yoo
mesh_terms: D008735:5-Methoxytryptamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D000818:Animals; D002853:Chromatography, Liquid; D015198:Designer Drugs / Q000737:chemistry; D053593:Forensic Toxicology; D006213:Hallucinogens / Q000737:chemistry / Q000652:urine*; D051381:Rats; D012701:Serotonin / Q000031:analogs & derivatives / Q000737:chemistry / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C568611:5-hydroxy-N,N-diisopropyltryptamine; C487454:5-methoxy-N-isopropyltryptamine; D015198:Designer Drugs; D006213:Hallucinogens; C033696:5-methoxy-N,N-diisopropyltryptamine; D012701:Serotonin; D008735:5-Methoxytryptamine