title: Impact of local versus global ligand density on cellular adhesion.
abstract: α(v)β(3) integrin-mediated cell adhesion is crucially influenced by how far ligands are spaced apart. To evaluate the impact of local ligand density versus global ligand density of a given surface, we used synthetic micronanostructured cell environments with user-defined ligand spacing and patterns to investigate cellular adhesion. The development of stable focal adhesions, their number, and size as well as the cellular adhesion strength proved to be influenced by local more than global ligand density.
authors.affiliation: Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart, Germany.
authors.forename: Janosch A
authors.initials: JA
authors.lastname: Deeg
authors.forename: Ilia
authors.initials: I
authors.lastname: Louban
authors.forename: Daniel
authors.initials: D
authors.lastname: Aydin
authors.forename: Christine
authors.initials: C
authors.lastname: Selhuber-Unkel
authors.forename: Horst
authors.initials: H
authors.lastname: Kessler
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology*; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D051381:Rats
chemical_list: D020099:Coated Materials, Biocompatible; D039302:Integrin alphaVbeta3