title: Fe/Mn superoxide dismutase-encoding gene in Paracoccus denitrificans is induced by azide and expressed independently of the FNR-type regulators.
abstract: Paracoccus denitrificans cells undergo changes in protein composition upon exposure to azide, a known activator of the fumarate-nitrate reduction (FNR)-type transcription factor NarR. One of the most prominent protein species inducible by azide is a Fe/Mn-family superoxide dismutase (SOD). Azide induces SOD at protein, mRNA transcript, and enzyme activity levels in the aerobically growing cells. Since SOD expression remains unaffected in the fnrP-, nnr-, and narR-mutant strains, we postulate a mechanism independent of the known FNR-type regulators but involving a redox signal arising from the respiratory chain.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500, Brno, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Bouchal
authors.forename: T
authors.initials: T
authors.lastname: Vyhlídalová
authors.forename: I
authors.initials: I
authors.lastname: Struhárová
authors.forename: Z
authors.initials: Z
authors.lastname: Zdráhal
authors.forename: I
authors.initials: I
authors.lastname: Kučera
mesh_terms: D000595:Amino Acid Sequence; D001386:Azides / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D010231:Paracoccus denitrificans / Q000201:enzymology* / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D001386:Azides; D001426:Bacterial Proteins; D013482:Superoxide Dismutase