title: Identification of obstructive jaundice-related microRNAs in mouse liver.
abstract: OBJECTIVE
  Although microRNAs are known to be post-transcriptional regulators in physiological and pathological events in the liver, their role in the obstructive jaundice liver remains unclear.
  
  
  METHODS
  We sequenced the small RNA libraries of the bile duct ligation (BDL) mouse liver to detect the in vivo microRNA expression profiles of obstructive jaundice. We also validated the differential expression of microRNAs in the BDL liver using real-time PCR. Laser microdissection was performed to identify the origin of BDL-related microRNAs. An IL6-treated normal intrahepatic biliary epithelial cell line was used as an in vitro model of obstructive jaundice.
  
  
  RESULTS
  We found microRNAs that were upregulated in the BDL liver (let-7a, let-7d, let-7f, let-7g, miR-21, miR-125a-5p, miR-125b-5p, miR-194, miR-199a-3p, miR-199a-5p, miR-214, miR-221, and miR-486). Furthermore, laser-microdissection analysis showed that miR-199a-5p was significantly upregulated in the intrahepatic bile duct of the BDL liver. The in vitro expression of miR-199a-5p was appreciably elevated in accordance with increased proliferation of IL6-treated cells.
  
  
  CONCLUSIONS
  We revealed dynamic changes in microRNA expression during obstructive jaundice using the BDL model. MiR-199a-5p was likely associated with the proliferation of intrahepatic bile ducts. Our data will facilitate further study of the pathophysiological role(s) of microRNAs in the obstructive jaundice liver.
authors.affiliation: Department of Surgery for Organ Function and Biological Regulation, Nippon Medical School, Tokyo, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kanda
authors.forename: Osamu
authors.initials: O
authors.lastname: Ishibashi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawahigashi
authors.forename: Takuya
authors.initials: T
authors.lastname: Mishima
authors.forename: Takuji
authors.initials: T
authors.lastname: Kosuge
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Mizuguchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Arima
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yokomuro
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Takashi
authors.initials: T
authors.lastname: Tajiri
authors.forename: Eiji
authors.initials: E
authors.lastname: Uchida
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Takizawa
mesh_terms: D000818:Animals; D015850:Interleukin-6 / Q000494:pharmacology; D041781:Jaundice, Obstructive / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D016133:Polymerase Chain Reaction
chemical_list: D015850:Interleukin-6; D035683:MicroRNAs; C534780:Mirn199 microRNA, mouse