title: [The expression and significance of RKIP in lung squamous cell carcinoma tissues].
abstract: OBJECTIVE
  Raf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanolamine binding protein (PEBP) family. RKIP is an endogenous inhibitor of the Raf-1-MEK1/2-ERK1/2 signaling pathway, NFkappaB signaling pathway and G protein coupled receptors signaling pathway. The aim of this study is to explore the expression of RKIP in lung squamous cell carcinoma, and the relationship between RKIP expression and clinical pathology of lung squamous cell carcinoma.
  
  
  METHODS
  The expression of RKIP mRNA was detected in 56 lung squamous cell carcinoma tissues and adjacent cancer tissues by RT-PCR. The expression level of RKIP protein was detected by Western blot. The positive rates of RKIP expression in cancer tissues and adjacent cancer tissues were analyzed.
  
  
  RESULTS
  RKIP mRNA positive expression rate in cancer tissues was significantly lower than that in adjacent cancer tissues (P < 0.05). The positive rate of RKIP mRNA expression in the cancer tissues with lymph node metastasis was significantly lower than that without lymph node metastasis (P < 0.05). RKIP mRNA expression has no remarkable correlation with age, sex, tumor differentiation and size (P > 0.05). RKIP protein expression in the cancer tissues was significantly lower than that in adjacent cancer tissues (P < 0.05).
  
  
  CONCLUSIONS
  The deficiency of RKIP expression is positively correlated with carcinogenesis and invasion metastasis of lung squamous cell carcinoma.
authors.affiliation: Department of Medical Oncology, the First People's Hospital of Kunshan, Affiliated to Jiangsu University, Kunshan, China. alain15024@gmail.com
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Shen
authors.forename: Liqiang
authors.initials: L
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.forename: Minbin
authors.initials: M
authors.lastname: Chen
authors.forename: Lina
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C511482:PEBP1 protein, human; D049488:Phosphatidylethanolamine Binding Protein