title: A three-layer competition-based giant magnetoresistive assay for direct quantification of endoglin from human urine.
abstract: This study presents a three-layer competition-based assay for ultrasensitive detection and quantification of endoglin from unprocessed human urine samples using a giant magnetoresistive (GMR) sensor and high-moment magnetic nanoparticle-based biosensing technology. This biosensing platform detects as few as 1000 copies of endoglin at concentrations as low as 83 fM with high detection specificity and has a three-order dynamic range. The results reveal that endoglin levels in urine have the potential to predict for the presence of prostate cancer and to distinguish between prostate cancers of different grades.
authors.affiliation: Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Srinivasan
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Jing
authors.forename: Chengguo
authors.initials: C
authors.lastname: Xing
authors.forename: Joel
authors.initials: J
authors.lastname: Slaton
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wang
mesh_terms: D015703:Antigens, CD / Q000652:urine*; D015374:Biosensing Techniques / Q000379:methods*; D000071063:Endoglin; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry; D011956:Receptors, Cell Surface; D012680:Sensitivity and Specificity
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface