title: The characterisation of lactic acid bacteria during the fermentation of an artisan Serbian sausage (Petrovská Klobása).
abstract: Petrovská Klobása is an artisan Serbian sausage made only from meat and spices without any additives or starter cultures. In order to characterise lactic acid bacteria (LAB) microflora, a total number of 404 LAB strains were isolated from 15 samples collected during 90 days of the fermentation and 120 days of storage of one batch of Petrovská Klobása. The isolates were preliminarily identified by phenotypic tests and subjected to (GTG)₅-PCR fingerprinting. Representatives of each group were identified by 16S rDNA sequencing. The results showed that among the isolates, Lactobacillus sakei and Leuconostoc mesenteroides predominate with 36.4% and 37.1% of total LAB strains, respectively. Pediococcus pentosaceus was also isolated in high proportion (18.4%) whereas Enterococcus durans and Enterococcus caseliflavus made only 1% and 6% of the total isolates, correspondingly. The analysis of vacuum packed and modified atmosphere packed (MAP) samples showed higher presence of L. mesenteroides and L. sakei in the total microflora.
authors.affiliation: Faculty of Technology, Bulevar Oslobodjenja 124, 16000 Leskovac, Serbia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Danilović
authors.forename: Nataša
authors.initials: N
authors.lastname: Joković
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Petrović
authors.forename: Katarina
authors.initials: K
authors.lastname: Veljović
authors.forename: Maja
authors.initials: M
authors.lastname: Tolinački
authors.forename: D
authors.initials: D
authors.lastname: Savić
mesh_terms: D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology; D018857:Food Packaging; D005519:Food Preservation; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification*; D007934:Leuconostoc / Q000235:genetics / Q000302:isolation & purification*; D008461:Meat Products / Q000032:analysis / Q000382:microbiology*; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D055771:Serbia; D014618:Vacuum
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S