title: Correlation of clinical trachoma and infection in Aboriginal communities.
abstract: BACKGROUND
  Trachoma is the leading infectious cause of blindness due to conjunctival infection with Chlamydia trachomatis. The presence of active trachoma and evidence of infection are poorly correlated and a strong immunologically-mediated inflammatory response means that clinical signs last much longer than infection. This population-based study in five Aboriginal communities endemic for trachoma in northern Australia compared a fine grading of clinical trachoma with diagnostic positivity and organism load.
  
  
  METHODS
  A consensus fine grading of trachoma, based on clinical assessment and photograding, was compared to PCR, a lipopolysacharide (LPS)-based point-of-care (POC) and a 16S RNA-based nucleic acid amplification test (NAAT). Organism load was measured in PCR positive samples.
  
  
  RESULTS
  A total of 1282 residents, or 85.2% of the study population, was examined. Taking the findings of both eyes, the prevalence of trachomatous inflammation-follicular (TF) in children aged 1-9 years was 25.1% (96/383) of whom 13 (13.7%) were PCR positive on the left eye. When clinical data were limited to the left eye as this was tested for PCR, the prevalence of TF decreased to 21.4% (82/383). The 301 TF negative children, 13 (4.3%) were PCR positive. The fine grading of active trachoma strongly correlated with organism load and disease severity (rs = 0.498, P = 0.0004). Overall, 53% of clinical activity (TF(1) or TF(2)) and 59% of PCR positivity was found in those with disease scores less than the WHO simplified grade of TF.
  
  
  CONCLUSIONS
  Detailed studies of the pathogenesis, distribution and natural history of trachoma should use finer grading schemes for the more precise identification of clinical status. In low prevalence areas, the LPS-based POC test lacks the sensitivity to detect active ocular infection and nucleic acid amplification tests such as PCR or the 16S-RNA based NAAT performed better. Trachoma in the Aboriginal communities requires specific control measures.
authors.affiliation: Diagnostics Development Unit, Department of Haematology, University of Cambridge, National Health Service Blood and Transplant Site, Cambridge, UK.
authors.forename: Claude-Edouard C
authors.initials: CE
authors.lastname: Michel
authors.forename: Katrina G
authors.initials: KG
authors.lastname: Roper
authors.forename: Magda A
authors.initials: MA
authors.lastname: Divena
authors.forename: Helen H
authors.initials: HH
authors.lastname: Lee
authors.forename: Hugh R
authors.initials: HR
authors.lastname: Taylor
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001315:Australia; D002648:Child; D002675:Child, Preschool; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000473:pathology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods; D012680:Sensitivity and Specificity; D014141:Trachoma / Q000175:diagnosis* / Q000473:pathology; D055815:Young Adult