title: Development of reagents and assays for the detection of pathogenic Burkholderia species.
abstract: Rapid detection of the category B biothreat agents Burkholderia pseudomallei and Burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for B. pseudomallei). We are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. We present our current medium-throughout approach for target selection and production of Burkholderia macromolecules and describe the generation of a Fab molecule targeted to the B. mallei BimA protein. We also present development of prototype assays for detecting Burkholderia species using anti-lipopolysaccharide antibodies.
authors.affiliation: Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, Texas 78712, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Qazi
authors.forename: Mridula
authors.initials: M
authors.lastname: Rani
authors.forename: Annie J
authors.initials: AJ
authors.lastname: Gnanam
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Cullen
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Stead
authors.forename: Haley
authors.initials: H
authors.lastname: Kensing
authors.forename: Kate
authors.initials: K
authors.lastname: McCaul
authors.forename: Sarah
authors.initials: S
authors.lastname: Ngugi
authors.forename: Joann L
authors.initials: JL
authors.lastname: Prior
authors.forename: Alexandria
authors.initials: A
authors.lastname: Lipka
authors.forename: Judit M
authors.initials: JM
authors.lastname: Nagy
authors.forename: C Whitlock
authors.initials: CW
authors.lastname: Gregory
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Judy
authors.forename: Sarah V
authors.initials: SV
authors.lastname: Harding
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
authors.forename: Sachdev S
authors.initials: SS
authors.lastname: Sidhu
authors.forename: M Stephen
authors.initials: MS
authors.lastname: Trent
authors.forename: G Barrie
authors.initials: GB
authors.lastname: Kitto
authors.forename: Alfredo
authors.initials: A
authors.lastname: Torres
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
authors.forename: Brent
authors.initials: B
authors.lastname: Iverson
authors.forename: George
authors.initials: G
authors.lastname: Georgiou
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Brown
mesh_terms: D000818:Animals; D042726:Burkholderia mallei / Q000302:isolation & purification* / Q000378:metabolism; D016957:Burkholderia pseudomallei / Q000302:isolation & purification* / Q000378:metabolism; D018834:Chaperonin 60 / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005896:Glanders / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis / Q000382:microbiology*; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: C503173:BimA protein, Burkholderia pseudomallei; D018834:Chaperonin 60; D008840:Microfilament Proteins