title: Preparation, release profiles and antibacterial properties of vancomycin-loaded Ca-P coating titanium alloy plate.
abstract: The aim of the study was to explore the feasibility of the Ca-P coating titanium alloy plate to be used as the vancomycin drug-delivery system by biomimetic coating technology. Through the X-ray diffraction study, the main components of the coatings were identified as octocalcium phosphate. The in vitro vancomycin release, bacteriostasis activity to Staphylococcus aureus (S. aureus), the scanning electron microscope (SEM) image and osteoblast adhesion and proliferation test of vancomycin-loaded Ca-P coating plate were evaluated. The bacteriostatic activity of the vancomycin-loaded Ca-P coating plate showed a continuous drug release and had an inhibitory effect on the growth of the S. aureus. In vitro osteoblast culture results showed that the Ca-P coating plate loaded with or without the vancomycin both obviously promoted the osteoblast attachment. It was suggested that the vancomycin-loaded Ca-P coating may be compounded in the surface of the internal fixators to reduce the incidence of the implant-associated infection.
authors.affiliation: Department of Orthopedics, General Hospital of Chinese People's Liberation Army, Beijing 100853, China feijundoctor@sohu.com
authors.forename: Jun
authors.initials: J
authors.lastname: Fei
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liu
authors.forename: Chang-jiang
authors.initials: CJ
authors.lastname: Pan
authors.forename: Ji-ying
authors.initials: JY
authors.lastname: Chen
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: Zhou
authors.forename: Song-hua
authors.initials: SH
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-jun
authors.initials: HJ
authors.lastname: Yu
mesh_terms: D000497:Alloys / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion; D002130:Calcium Phosphates / Q000737:chemistry; D049109:Cell Proliferation; D016503:Drug Delivery Systems; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning / Q000379:methods; D015233:Models, Statistical; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D051381:Rats; D013211:Staphylococcus aureus / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*; D014640:Vancomycin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D014640:Vancomycin; C020243:calcium phosphate; C511195:norvancomycin; D014025:Titanium