title: Strategy of controlling the volumetric loading rate to promote hydrogen-production performance in a mesophilic-kitchen-waste fermentor and the microbial ecology analyses.
abstract: The kitchen waste was chosen as a high solid (42 gL(-1) of volatile suspended solid, VSS) and high organic (107 gL(-1) of chemical oxygen demand) feedstock for operating a 3-L mesophilic fermentor. The greatest specific hydrogen production rate ( r(H2) was observed in Stage 3 as 3.4 L-H2 L(-1) day(-1) with a volumetric loading rate (VLR) of 100 g-CODL(-1) day(-1); the highest hydrogen yield was observed in Stage 2 as 96 mL-H2 g(-1) of influent VSS with a VLR of 46 g-COD L(-1) day(-1). In Stages 1 (with a VLR of 27 g-COD L(-1)) and 2, the sum of Butyrivibrio fibrisolvens and Clostridium proteoclasticum is dominant, but in Stage 3, Olsenella genomosp, became dominant and constituted 44% of the entire population. The dependence of VLR and r(H2)could be regressed as a linear equation of r(H2) = (2.83 VLR + 40.5) x 10(-2) .
authors.affiliation: Department of Environmental Engineering, National Cheng Kung University, No. 1 University Road, Tainan 701, Taiwan, ROC.
authors.forename: Shiue-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Lin
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Wang
authors.forename: Ze-Kun
authors.initials: ZK
authors.lastname: Lee
authors.forename: Shih-Chiang
authors.initials: SC
authors.lastname: Kuo
authors.forename: I-Cheng
authors.initials: IC
authors.lastname: Tseng
authors.forename: Sheng-Shung
authors.initials: SS
authors.lastname: Cheng
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D005285:Fermentation / Q000502:physiology*; D006859:Hydrogen / Q000378:metabolism*; D006865:Hydrogenation; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D013997:Time Factors; D014865:Waste Disposal, Fluid; D014866:Waste Products / Q000032:analysis*
chemical_list: D056804:Biofuels; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014866:Waste Products; D006859:Hydrogen