title: Excess no predisposes mitochondrial succinate-cytochrome c reductase to produce hydroxyl radical.
abstract: Mitochondria-derived oxygen-free radical(s) are important mediators of oxidative cellular injury. It is widely hypothesized that excess NO enhances O(2)(•-) generated by mitochondria under certain pathological conditions. In the mitochondrial electron transport chain, succinate-cytochrome c reductase (SCR) catalyzes the electron transfer reaction from succinate to cytochrome c. To gain the insights into the molecular mechanism of how NO overproduction may mediate the oxygen-free radical generation by SCR, we employed isolated SCR, cardiac myoblast H9c2, and endothelial cells to study the interaction of NO with SCR in vitro and ex vivo. Under the conditions of enzyme turnover in the presence of NO donor (DEANO), SCR gained pro-oxidant function for generating hydroxyl radical as detected by EPR spin trapping using DEPMPO. The EPR signal associated with DEPMPO/(•)OH adduct was nearly completely abolished in the presence of catalase or an iron chelator and partially inhibited by SOD, suggesting the involvement of the iron-H(2)O(2)-dependent Fenton reaction or O(2)(•-)-dependent Haber-Weiss mechanism. Direct EPR measurement of SCR at 77K indicated the formation of a nonheme iron-NO complex, implying that electron leakage to molecular oxygen was enhanced at the FAD cofactor, and that excess NO predisposed SCR to produce (•)OH. In H9c2 cells, SCR-dependent oxygen-free radical generation was stimulated by NO released from DEANO or produced by the cells following exposure to hypoxia/reoxygenation. With shear exposure that led to overproduction of NO by the endothelium, SCR-mediated oxygen-free radical production was also detected in cultured vascular endothelial cells.
authors.affiliation: Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Chen
authors.forename: Chwen-Lih
authors.initials: CL
authors.lastname: Chen
authors.forename: B Rita
authors.initials: BR
authors.lastname: Alevriadou
authors.forename: Jay L
authors.initials: JL
authors.lastname: Zweier
authors.forename: Yeong-Renn
authors.initials: YR
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004048:Diethylamines / Q000494:pharmacology; D004563:Electrochemistry; D004578:Electron Spin Resonance Spectroscopy; D042783:Endothelial Cells / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D032446:Myoblasts / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology*; D030421:Peroxynitrous Acid / Q000378:metabolism; D051381:Rats; D013384:Succinate Cytochrome c Oxidoreductase / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D004048:Diethylamines; D013481:Superoxides; D030421:Peroxynitrous Acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C034281:diethylamine; D013384:Succinate Cytochrome c Oxidoreductase