title: Complete nucleic acid sequence of Penaeus stylirostris densovirus (PstDNV) from India.
abstract: Infectious hypodermal and hematopoietic necrosis virus (IHHNV) of shrimp, recently been classified as Penaeus stylirostris densovirus (PstDNV). The complete nucleic acid sequence of PstDNV from India was obtained by cloning and sequencing of different DNA fragment of the virus. The genome organisation of PstDNV revealed that there were three major coding domains: a left ORF (NS1) of 2001 bp, a mid ORF (NS2) of 1092 bp and a right ORF (VP) of 990 bp. The complete genome and amino acid sequences of three proteins viz., NS1, NS2 and VP were compared with the genomes of the virus reported from Hawaii, China and Mexico and with partial sequence available from isolates from different regions. The phylogenetic analysis of shrimp, insect and vertebrate parvovirus sequences showed that the Indian PstDNV isolate is phylogenetically more closely related to one of the three isolates from Taiwan (AY355307), and two isolates (AY362547 and AY102034) from Thailand.
authors.affiliation: Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575 002, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Rai
authors.forename: Muhammed P
authors.initials: MP
authors.lastname: Safeena
authors.forename: Iddya
authors.initials: I
authors.lastname: Karunasagar
authors.forename: Indrani
authors.initials: I
authors.lastname: Karunasagar
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D017990:Densovirus / Q000235:genetics* / Q000302:isolation & purification; D023061:Gene Order; D007194:India; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D033561:Penaeidae / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins