title: Differentiation of MC3T3-E1 on poly(4-styrenesulfonic acid-co-maleic acid)sodium salt-coated films.
abstract: Polyelectrolyte multilayer (PEM) film can modify the surface properties of materials to improve cellular responses. In this study poly(diallyldimethylammonium chloride) (PDADMAC), poly(sodium 4-styrene sulfonate) (PSS) and poly(4-styrenesulfonic acid-co-maleic acid) sodium salt (PSS-co-MA) were assembled into PEM {(PDADMAC/PSS)(4)/PDADMAC+PSS-co-MA} film on glass surfaces and its ability on affecting osteoblast functions were examined. PSS-co-MA film showed an increase roughness and more wettable surface as compared to glass. When the osteoblast cell line, MC3T3-E1, was seeded on the surfaces, no differences were observed in cell attachment or spreading on either PSS-co-MA film or glass at 4-16 hours. However, increases in alkaline phosphatase activity (day-5 and 7) and the expression of osteocalcin mRNA/protein at day-13 were observed. Cells cultured on PSS-co-MA film developed a faster rate of calcium deposition at day-15 compared to control. In conclusion, PSS-co-MA film enhanced osteoblast differentiation and could be used to promote mineralization and improve osseointegration for dental implants.
authors.affiliation: Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
authors.forename: Thidarat
authors.initials: T
authors.lastname: Angwarawong
authors.forename: Stephan T
authors.initials: ST
authors.lastname: Dubas
authors.forename: Mansuang
authors.initials: M
authors.lastname: Arksornnukit
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002021:Buffers; D002113:Calcification, Physiologic / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape; D002470:Cell Survival / Q000502:physiology; D005898:Glass / Q000737:chemistry; D008298:Maleates / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000032:analysis; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013451:Sulfonic Acids / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D002021:Buffers; D008298:Maleates; D011095:Polyethylenes; D011108:Polymers; D011137:Polystyrenes; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; C518261:poly(4-styrenesulfonic acid-co-maleic acid); D015675:Osteocalcin; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D000469:Alkaline Phosphatase; C077114:styrenesulfonic acid polymer