title: Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV.
abstract: Laminin receptor (Lamr) in shrimp was previously proposed to be a potential receptor protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since shrimp Lamr bound to the VP1 capsid protein of TSV, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to Lamr. Thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. These were Penaeus stylirostris densovirus (PstDNV), white spot syndrome virus (WSSV), infectious myonecrosis virus (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head virus (YHV). Protein interaction analysis using yeast two-hybrid assay revealed that Lamr specifically interacted with capsid/envelope proteins of RNA viruses IMNV and YHV but not MrNV and not with the capsid/envelope proteins of DNA viruses PstDNV and WSSV. In vitro pull-down assay also confirmed the interaction between Lamr and YHV gp116 envelope protein, and injection of recombinant Lamr (rLamr) protein produced in yeast cells protected shrimp against YHV in laboratory challenge tests.
authors.affiliation: Center for Genomic and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Nattaphon
authors.initials: N
authors.lastname: Busayarat
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.forename: Moltira
authors.initials: M
authors.lastname: Tonganunt
authors.forename: Kornsunee
authors.initials: K
authors.lastname: Phiwsaiya
authors.forename: Watcharachai
authors.initials: W
authors.lastname: Meemetta
authors.forename: Sasimanas
authors.initials: S
authors.lastname: Unajak
authors.forename: Sarawut
authors.initials: S
authors.lastname: Jitrapakdee
authors.forename: Chu-Fang
authors.initials: CF
authors.lastname: Lo
authors.forename: Amornrat
authors.initials: A
authors.lastname: Phongdara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D033561:Penaeidae / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D012328:RNA Viruses / Q000378:metabolism* / Q000502:physiology; D017474:Receptors, Laminin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D046828:Roniviridae / Q000378:metabolism* / Q000502:physiology; D020798:Two-Hybrid System Techniques; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D017474:Receptors, Laminin; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins