title: Ovarian steroids modulate tumor necrosis factor-α and nitric oxide-regulated prostaglandin secretion by cultured bovine oviductal epithelial cells.
abstract: Ovarian steroids assure an optimum environment for the final maturation of oocytes, gamete transport, fertilization, and early embryonic development. The aim of experiment 1 was to examine the influence of ovarian steroids on tumor necrosis factor-α (TNF-α)- or nitric oxide (NO)-regulated prostaglandin (PG), and nitrite/nitrate (NO₂/NO₃) secretion by cultured bovine oviductal epithelial cells (BOECs). BOECs were pretreated with 17β-estradiol (E₂; 10⁻⁹ M) and/or progesterone (P₄; 10⁻⁷ M) for 24 h. For the next 24 h, BOECs were treated with TNF-α (10 ng/mL) or spermine nitric oxide complex (NONOate; 10⁻⁵ M). Prostaglandin F(2α) and PGE₂ secretion was measured in medium by ELISA. The pretreatment of cells with P₄ (progesterone), E₂ (17 β-estradiol), or E₂/P₄ augmented TNF-α-induced PGF(2α) and PGE₂ secretion (P < 0.01). The pretreatment of cells with E₂ or E₂/P₄ increased NONOate-induced PGF(2α) and PGE₂ secretion (P < 0.01). TNF-α induced NO₂/NO₃ production by BOECs. The pretreatment of cells with E₂ augmented only TNF-α-induced NO₂/NO₃ production (P < 0.05). The aim of experiment 2 was to examine the influence of TNF-α, NO, and ovarian steroids on the protein content of enzymes specifically involved in PG and NO production, PG synthases, and NO synthases (NOSs). BOECs were treated with TNF-α (10 ng/mL) or NONOate (10⁻⁵ M). TNF-α increased the protein content of PGG/H synthase, PGF synthase, and PGE synthase (P < 0.05) and endothelial and inducible NOSs (P < 0.05). Nitric oxide increased the protein content of PGF synthase, PGE synthase, endothelial NOS, and inducible NOS (P < 0.05). These results show possible linkage between TNF-α and NO, modulated by ovarian steroids, in the regulation of PG synthesis by BOECs that may be important for triggering the process of oviductal contractions.
authors.affiliation: Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn 10-747, Poland.
authors.forename: A Z
authors.initials: AZ
authors.lastname: Szóstek
authors.forename: M J
authors.initials: MJ
authors.lastname: Siemieniuch
authors.forename: K
authors.initials: K
authors.lastname: Deptula
authors.forename: I
authors.initials: I
authors.lastname: Woclawek-Potocka
authors.forename: M
authors.initials: M
authors.lastname: Majewska
authors.forename: K
authors.initials: K
authors.lastname: Okuda
authors.forename: D J
authors.initials: DJ
authors.lastname: Skarzynski
mesh_terms: D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005187:Fallopian Tubes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis; D019746:Intramolecular Oxidoreductases / Q000032:analysis; D009569:Nitric Oxide / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000032:analysis; D011374:Progesterone / Q000494:pharmacology*; D000071876:Prostaglandin-E Synthases; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis; D011453:Prostaglandins / Q000096:biosynthesis / Q000378:metabolism*; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D011453:Prostaglandins; D014409:Tumor Necrosis Factor-alpha; C091861:spermine nitric oxide complex; D013096:Spermine; D009569:Nitric Oxide; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone