title: [Relationship between WT1-specific T-cell subsets and graft-versus-host disease after nonmyeloablative allogeneic transplantation].
abstract: OBJECTIVE
  To explore the relationship between WT1-induced T-cell subsets and graft-versus-host disease (GVHD) after nonmyeloablative allogeneic hematopoietic stem cell transplantation (NST).
  
  
  METHODS
  Peripheral blood mononucleated cells (PBMCs) from 19 patients who expressed WT1 and developed GVHD after NST were simulated by WT1126-134 peptide in vitro, and proportions of WT1-induced-T-cell subsets (Tc1, Tc2, Th1, Th2 cells) before and after transplant were detected by intracellular cytokine staining (ICCS) assay. WT1-specific CD8(+) CTLs of 14 patients with HLA-A*0201 were detected by HLA-A*0201/WT1 pentamer.
  
  
  RESULTS
  (1) 17 of 19 patients developed GVHD, among whom proportions of Tc1 and Th1 cells, achieved peak value in 16 patients at occurrence of GVHD (P = 0.039); (2) The peak proportions of Tc1 and Th1 cells in patients with aGVHD above grade II were higher than those with grade I, but being no statistical difference (P = 0.900 and P = 0.140, respectively); (3) The peak proportion of Th1 cells (P = 0.004), but not Tc1 cells (P = 0.060) in patients with extensive cGVHD was significantly higher than that in patients with limited one; (4) Proportions of Tc1, Th1 and WT1(+)CD8(+)CTL in patients without GVHD were similar to those in patients with Grade I aGVHD, but lower than those in aGVHD above grade II.
  
  
  CONCLUSIONS
  GVHD promotes the generation of WT1-induced GVL effect, and the intensity of the latter maybe correlated with the intensity of GVHD, especially cGVHD. Th1 cells play a more important role in the enhancement of WT1-induced GVL effect in extensive cGVHD patient than in limited cGVHD patients.
authors.affiliation: Department of Hematology, Affiliated Hospital of Academy of Military Medicine Science, Beijing 100071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Hong-li
authors.initials: HL
authors.lastname: Zuo
authors.forename: Tie-qiang
authors.initials: TQ
authors.lastname: Liu
authors.forename: Xue-dong
authors.initials: XD
authors.lastname: Sun
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Guang-xian
authors.initials: GX
authors.lastname: Liu
authors.forename: Qi-yun
authors.initials: QY
authors.lastname: Sun
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Qiao
authors.forename: Dan-hong
authors.initials: DH
authors.lastname: Wang
authors.forename: Chang-lin
authors.initials: CL
authors.lastname: Yu
authors.forename: Kai-xun
authors.initials: KX
authors.lastname: Hu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Dong
authors.forename: Hui-sheng
authors.initials: HS
authors.lastname: Ai
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology*; D020544:Graft vs Leukemia Effect; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014184:Transplantation, Homologous; D025721:WT1 Proteins / Q000378:metabolism*; D055815:Young Adult
chemical_list: D025721:WT1 Proteins