title: Combined effects of surface morphology and mechanical straining magnitudes on the differentiation of mesenchymal stem cells without using biochemical reagents.
abstract: Existing studies examining the control of mesenchymal stem cell (MSC) differentiation into desired cell types have used a variety of biochemical reagents such as growth factors despite possible side effects. Recently, the roles of biomimetic microphysical environments have drawn much attention in this field. We studied MSC differentiation and changes in gene expression in relation to osteoblast-like cell and smooth muscle-like cell type resulting from various microphysical environments, including differing magnitudes of tensile strain and substrate geometries for 8 days. In addition, we also investigated the residual effects of those selected microphysical environment factors on the differentiation by ceasing those factors for 3 days. The results of this study showed the effects of the strain magnitudes and surface geometries. However, the genes which are related to the same cell type showed different responses depending on the changes in strain magnitude and surface geometry. Also, different responses were observed three days after the straining was stopped. These data confirm that controlling microenvironments so that they mimic those in vivo contributes to the differentiation of MSCs into specific cell types. And duration of straining engagement was also found to play important roles along with surface geometry.
authors.affiliation: R&D Center, Genewel Co., Ltd., Sangdaewon 1-dong, Jungwon-gu, Seongnam, Gyeonggi-do 462-724, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Jang
authors.forename: Shi Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: So Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Shin
authors.forename: ChiWoong
authors.initials: C
authors.lastname: Mun
authors.forename: Su-Hyang
authors.initials: SH
authors.lastname: Kim
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Kim
authors.forename: Jung-Woog
authors.initials: JW
authors.lastname: Shin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011817:Rabbits; D013499:Surface Properties; D013718:Tensile Strength