title: Fading of auramine-stained mycobacterial smears and implications for external quality assurance.
abstract: Light-emitting diode fluorescence microscopy is being scaled up for tuberculosis control, but fading of auramine-stained slides could compromise external quality assurance. We stored auramine-stained slides and reexamined them over time. Slides stored in all environments faded quickly, with significant changes in the proportion of positive slides in as little as 1 week.
authors.affiliation: Department of Epidemiology, McGill University, Montreal, Canada.
authors.forename: Jessica
authors.initials: J
authors.lastname: Minion
authors.forename: Shubhada
authors.initials: S
authors.lastname: Shenai
authors.forename: Viral
authors.initials: V
authors.lastname: Vadwai
authors.forename: Tejashree
authors.initials: T
authors.lastname: Tipnis
authors.forename: Christina
authors.initials: C
authors.lastname: Greenaway
authors.forename: Dick
authors.initials: D
authors.lastname: Menzies
authors.forename: Andrew
authors.initials: A
authors.lastname: Ramsay
authors.forename: Camilla
authors.initials: C
authors.lastname: Rodrigues
authors.forename: Madhukar
authors.initials: M
authors.lastname: Pai
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards*; D001576:Benzophenoneidum / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods / Q000592:standards; D011785:Quality Assurance, Health Care; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards*
chemical_list: D001576:Benzophenoneidum