title: [Anti-proliferative and anti-metastatic effects of curcumin on oral cancer cells].
abstract: OBJECTIVE
  The purpose of this article is to examine the effect of curcumin on the proliferation and metastasis of human tongue squamous cell carcinoma and analyze its mechanism.
  
  
  METHODS
  SCC-4 were treated with curcumin of 0, 5, 10, 20, 30, 60, 100 micromol x L(-1) in 24 h. MTT assay, Matrigel invasion assay, flow cytometry and fluorescence microscopy were used to examine the effect of curcumin on the growth and metastasis of SCC-4. cDNA microarray and RT-PCR were employed to analyze the expression of genes treated by curcumin.
  
  
  RESULTS
  The results showed that curcumin could concentration-dependently inhibit SCC-4 cell proliferation at the concentration range from 20 to 100 micromol x L(-1). Furthermore, Matrigel invasion assay indicated that curcumin can reduce SCC-4 cell invasion under the dosage of 20, 30, 60 micromol x L(-1). Flow cytometry also showed that curcumin can influence the distribution of cell cycle of SCC-4 cell with the dosage of 20, 30, 60 micromol x L(-1). And the dosage of 30 micromol x L(-1) curcumin could lead to the recruitment of alpha-tubulin. cDNA microarray showed that 87 genes were activated and 198 genes were inhibited with the effect of curcumin. These results were validated by the real time quantitative RT-PCR.
  
  
  CONCLUSIONS
  According to the results, it suggests that curcumin has the potential as the leading compound for anti-cancer proliferation and invasion in oral cancer treatment, and cdc27, EGFR substrate 15, PPAR-alpha and H2A histone may play an important role among this multiple anticancer-targeting ability.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
authors.forename: Jiao-wen
authors.initials: JW
authors.lastname: Chen
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Tang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Di
authors.initials: D
authors.lastname: Lü
authors.forename: Ning
authors.initials: N
authors.lastname: Geng
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003474:Curcumin; D006801:Humans; D009062:Mouth Neoplasms
chemical_list: D003474:Curcumin