title: Modification of a commercial Toxoplasma gondii immunoglobulin G enzyme-linked immunosorbent assay for use in multiple animal species.
abstract: A challenge faced by veterinary diagnosticians in serologic analysis for exposure to pathogens is the need for a protein conjugate capable of antibody attachment in many animal species. The advent of protein conjugates that are less specific in nature allows diagnosis across many species with little or no modification of technique. Toxoplasma gondii is an organism of veterinary interest that has been demonstrated to infect a plethora of warm-blooded animals. However, the serologic tests available for simultaneous diagnosis in this broad range are limited in number. The current study examined the use of an immunoglobulin G enzyme-linked immunosorbent assay (ELISA) modified by the use of non-species-specific protein conjugates in domestic animal species commonly submitted to diagnostic laboratories for evaluation of Toxoplasma exposure status. Comparison with results from an established indirect hemagglutination technique revealed very good agreement between the 2 test methods. This modification of the ELISA provides a useful method for veterinary diagnosticians to perform rapid and accurate evaluation of multiple animal species for Toxoplasma exposure using a single test.
authors.affiliation: Animal Health Diagnostic Center, Upper Tower Road, Cornell University, Ithaca, NY 14853, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Schaefer
authors.forename: Holly A
authors.initials: HA
authors.lastname: White
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Schaaf
authors.forename: Hussni O
authors.initials: HO
authors.lastname: Mohammed
authors.forename: Susan E
authors.initials: SE
authors.lastname: Wade
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007074:Immunoglobulin G / Q000032:analysis*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000097:blood / Q000469:parasitology*; D015047:Zoonoses / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G