title: Effect of ozone pretreatment on the microleakage of pit and fissure sealants.
abstract: OBJECTIVE
  This study investigated the effect of ozone pretreatment on the microleakage and marginal integrity of pit and fissure sealants placed with or without a self-etch 6th generation adhesive.
  
  
  METHODS
  Freshly-extracted, human third molars were randomly assigned into two main groups (n = 48): Group A: Fissures were pretreated with ozone; Group B: Fissures were left untreated. The teeth were further randomly divided into two subgroups (n = 24/each) so that half of teeth were sealed with a conventional fissure sealant (Fissurit F, Voco, Germany), while the remaining half received the same sealant bonded with a self-etch adhesive (Clearfil Protect Bond, Kuraray, Japan). Following thermal cycling (1000X), the specimens were subjected to dye penetration within 0.5% basic fuchsin for 24h. The extent of dye penetration was measured by image analysis. Kruskal Wallis and Mann-Whitney U tests were used for statistical analysis of the data (p = 0.05). Two randomly-selected sections from each group were observed under SEM RESULTS: In all groups, ozone pretreatment significantly reduced the extent of microleakage (p < 0.001). SEM investigation demonstrated better adaptation of the sealants in ozone-pretreated groups. Clearfil Protect Bond did not improve the marginal seal of Fissurit F (p > 0.05).
  
  
  CONCLUSIONS
  Ozone pretreatment favorably affected the marginal sealing ability of the tested fissure sealants.
authors.affiliation: Department of Pediatric Dentistry, Baskent University Faculty of Dentistry, Behcelievler, Ankara, Turkey. seviburcak@yahoo.com
authors.forename: S Burcak
authors.initials: SB
authors.lastname: Cehreli
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Yalcinkaya
authors.forename: Gunseli
authors.initials: G
authors.lastname: Guven-Polat
authors.forename: Zafer Cavit
authors.initials: ZC
authors.lastname: Cehreli
mesh_terms: D004396:Coloring Agents; D001840:Dental Bonding; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification*; D018772:Dental Marginal Adaptation; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D016877:Oxidants / Q000737:chemistry*; D010126:Ozone / Q000737:chemistry*; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D058626:Plasma Gases / Q000737:chemistry; D012394:Rosaniline Dyes; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: C503075:Clearfil Protect Bond; D004396:Coloring Agents; D017220:Dentin-Bonding Agents; C407603:Fissurit F; D008689:Methacrylates; D016877:Oxidants; D010895:Pit and Fissure Sealants; D058626:Plasma Gases; D012394:Rosaniline Dyes; C025485:basic fuchsin; D010126:Ozone