title: Effects of blue light irradiation on human dermal fibroblasts.
abstract: Previous studies have reported that separately from UV-radiation also blue light influences cellular physiology in different cell types. However, little is known about the blue light action spectrum. The purpose of this study was to investigate effects of blue light at distinct wavelengths (410, 420, 453, 480 nm) emitted by well defined light-emitting-diodes on viability, proliferation and antioxidative capacity of human dermal fibroblasts. We found that irradiation with blue light (410, 420 nm) led to intracellular oxidative stress and toxic effects in a dose and wavelength dependent manner. No toxicity was observed using light at 453 nm and 480 nm. Furthermore, blue light (410, 420, 453 nm) at low doses reduced the antioxidative capacity of fibroblasts. At non-toxic doses, irradiations at 410, 420 and 453 nm reduced proliferation indicating a higher susceptibility of proliferating fibroblasts to blue light. Our results show that blue light at different wavelengths may induce varying degrees of intracellular oxidative stress with different physiological outcome, which could contribute to premature skin photoaging. On the other hand, the use of blue light due to its antiproliferative and toxic properties may represent a new approach in treatment and prevention of keloids, hypertrophic scars and fibrotic skin diseases.
authors.affiliation: Interdisciplinary Center for Clinical Research (IZKF), Medical Faculty, RWTH Aachen University, Aachen, Germany. coplaender@ukaachen.de
authors.forename: Christian
authors.initials: C
authors.lastname: Opländer
authors.forename: Sarah
authors.initials: S
authors.lastname: Hidding
authors.forename: Frauke B
authors.initials: FB
authors.lastname: Werners
authors.forename: Matthias
authors.initials: M
authors.lastname: Born
authors.forename: Norbert
authors.initials: N
authors.lastname: Pallua
authors.forename: Christoph V
authors.initials: CV
authors.lastname: Suschek
mesh_terms: D000328:Adult; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D008027:Light; D008875:Middle Aged; D018384:Oxidative Stress