title: Bilayer lipid membrane formation on a chemically modified S-layer lattice.
abstract: The present paper describes the generation of a biomimetic model lipid membrane on bacterial surface (S-)layer which covered the entire surface of various sensors. The S-layer lattice allows one to be independent from the underlying solid material and provides a biological surface and anchoring structure for lipid membranes. S-layer proteins were chemically modified via binding of two amine-terminated phospholipids. Subsequently, a bimolecular lipid membrane anchored to the previously generated viscoelastic lipid monolayer was generated by the rapid solvent exchange technique. Characterization of the intermediate (monolayer) and final membrane structures (bilayer) was performed by imaging, surface-sensitive, and electrochemical techniques. This bilayer lipid membrane generated on an S-layer lattice revealed a thickness of ∼6 nm and constitutes a stable supported model membrane system with highly isolating properties showing a membrane resistance of 8.5 MΩ × cm(2).
authors.affiliation: Department for NanoBiotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 11, 1190 Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Schrems
authors.forename: A
authors.initials: A
authors.lastname: Kibrom
authors.forename: S
authors.initials: S
authors.lastname: Küpcü
authors.forename: E
authors.initials: E
authors.lastname: Kiene
authors.forename: U B
authors.initials: UB
authors.lastname: Sleytr
authors.forename: B
authors.initials: B
authors.lastname: Schuster
mesh_terms: D058266:Dielectric Spectroscopy; D004563:Electrochemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008567:Membranes, Artificial; D046529:Microscopy, Electron, Transmission; D008962:Models, Theoretical; D058525:Quartz Crystal Microbalance Techniques; D020349:Surface Plasmon Resonance
chemical_list: D008051:Lipid Bilayers; D008567:Membranes, Artificial