title: Synthesis, structural characterization, formation constants and in vitro cytotoxicity of phenanthroline and imidazolidine-2-thione copper(II) complexes.
abstract: The synthesis, crystal structures, physicochemical properties and complex formation constants of [Cu(phen)(2)(L)](ClO(4))(2) complexes, where phen is 1,10-ortho-phenanthroline and L is a series of substituted imidazolidine-2-thione, have been studied. Single crystal X-ray diffraction revealed a distorted trigonal-bipyramidal geometry for all the molecules. The complex formation constants were determined in nonaqueous media by spectrophotometric measurements. Testing copper(II) complexes in mouse neuroblastoma N2a cells persistently infected with the 22L strain of the scrapie prion protein (22L-N2a) resulted in high cytotoxicity but no antiprion activity at nontoxic doses.
authors.affiliation: Dipartimento di Scienze Chimiche, Università degli Studi di Cagliari, Monserrato (CA), Italy. tpivetta@unica.it
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pivetta
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Cannas
authors.forename: Francesco
authors.initials: F
authors.lastname: Demartin
authors.forename: Carlo
authors.initials: C
authors.lastname: Castellano
authors.forename: Sarah
authors.initials: S
authors.lastname: Vascellari
authors.forename: Gaetano
authors.initials: G
authors.lastname: Verani
authors.forename: Francesco
authors.initials: F
authors.lastname: Isaia
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003300:Copper / Q000737:chemistry*; D003601:Cytotoxicity Tests, Immunologic; D005031:Ethylenethiourea; D048289:Imidazolidines / Q000737:chemistry*; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D010618:Phenanthrolines / Q000737:chemistry*; D000072002:Prion Proteins; D011328:Prions / Q000378:metabolism; D013871:Thiones / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D002413:Cations, Divalent; D048289:Imidazolidines; D009942:Organometallic Compounds; D010618:Phenanthrolines; D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D013871:Thiones; D005031:Ethylenethiourea; D003300:Copper