title: Antimicrobial activities of indole alkaloids from Tabernaemontana catharinensis.
abstract: Tabernaemontana catharinensis root bark ethanol extract, EB2 fraction and the MMV alkaloid (12-methoxy-4-methylvoachalotine) were evaluated for their antimicrobial activities. T. catharinensis ethanol extract was effective against both strains of the dermatophyte Trichophyton rubrum at concentrations of 2.5 mg/mL (wild strain) and 1.25 mg/mL (mutant strain), while the EB2 fraction and MMV alkaloid showed a strong antifungal activity against wild and mutant strains with MIC values of <0.02 and 0.16 mg/mL, respectively. The EB2 fraction showed a strong antibacterial activity against ATCC strains of S. aureus, S. epidermidis, E. coli and P. aeruginosa with MICs from <0.02 to 0.04 mg/mL, as well as against resistant clinical isolates species of Enterococcus sp, Klebsiella oxytoca, Citrobacter, K. pneumoniae, P. mirabilis, S. aureus, S. epidermidis, E. coli and P. aeruginosa with MIC values ranging from 0.04 to 0.08 mg/mL. The MMV alkaloid presented a MIC of 0.16 mg/mL against the strains of S. aureus and E. coli ATCC. For the resistant clinical isolates Enterococcus sp, Citrobacter, S. aureus, S. epidermidis, E. coli and P. aeruginosa the MIC of MMV ranged from 0.08 to 0.31 mg/mL. The chromatography analysis of the EB2 fraction revealed the presence of indole alkaloids, including MMV, possibly responsible for the observed antimicrobial activity.
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, SP, Brasil.
authors.forename: Maria Rita Furquini
authors.initials: MR
authors.lastname: Medeiros
authors.forename: Luiz Afonso de Melo
authors.initials: LA
authors.lastname: Prado
authors.forename: Vanessa Colnaghi
authors.initials: VC
authors.lastname: Fernandes
authors.forename: Sérgio Souza
authors.initials: SS
authors.lastname: Figueiredo
authors.forename: Juliana
authors.initials: J
authors.lastname: Coppede
authors.forename: Juliana
authors.initials: J
authors.lastname: Martins
authors.forename: Giovana Maria Lanchoti
authors.initials: GM
authors.lastname: Fiori
authors.forename: Nilce Maria
authors.initials: NM
authors.lastname: Martinez-Rossi
authors.forename: Rene Oliveira
authors.initials: RO
authors.lastname: Beleboni
authors.forename: Silvia Helena Taleb
authors.initials: SH
authors.lastname: Contini
authors.forename: Paulo Sérgio
authors.initials: PS
authors.lastname: Pereira
authors.forename: Ana Lúcia
authors.initials: AL
authors.lastname: Fachin
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D026121:Indole Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology; D030014:Tabernaemontana / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D026121:Indole Alkaloids; D010936:Plant Extracts