title: Efficacy study and field application of an inactivated new type gosling viral enteritis virus vaccine for domestic geese.
abstract: New type gosling viral enteritis virus (NGVEV) caused a serious disease in naive juvenile goslings. In the described studies the performance of 2 vaccines was analyzed: a vaccine containing adjuvanted inactivated NGVEV and a vaccine containing adjuvanted inactivated NGVEV and recombinant goose IL-2. Breeder geese were subcutaneously vaccinated at the beginning of the egg production period with the vaccines. Breeder geese sham vaccinated with PBS served as control. The cellular and humoral immune responses of the vaccinated breeder geese, as well as the presence of maternally derived antibody to NGVEV, were investigated by ELISA, virus neutralization test, and lymphocyte proliferation assay, respectively. A significantly higher immunogenicity (P < 0.05) was induced by the inactivated NGVEV-recombinant goose IL-2 adjuvant vaccine compared with the inactivated NGVEV vaccine. The offspring of the vaccinated birds were challenged with virulent NGVEV (100 50% lethal dose) and the protective efficacy of the vaccines was determined. Furthermore, in a field trial the efficacy of the inactivated NGVEV vaccine was recorded from years 2003 to 2007. No clinical signs or abnormal health status were observed in the vaccinated breeder geese and the progeny. After a single application, >80% protection was shown in the progeny of geese vaccinated against NGVEV challenge for approximately 5 mo. The extensive field trials further demonstrated that vaccination of breeder geese with the inactivated NGVEV vaccine could be a safe and efficacious means to control NGVE disease. Moreover, the level of maternally derived NGVEV antibody titer in the egg yolk reflected the level of NGVEV antibodies in the breeder geese, suggesting that the egg yolk could be used to monitor the vaccination efficacy in commercial goose breeder flocks.
authors.affiliation: Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan Province, 625014, China.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: G P
authors.initials: GP
authors.lastname: Ma
authors.forename: M S
authors.initials: MS
authors.lastname: Wang
authors.forename: A C
authors.initials: AC
authors.lastname: Cheng
authors.forename: D K
authors.initials: DK
authors.lastname: Zhu
authors.forename: Q H
authors.initials: QH
authors.lastname: Luo
authors.forename: R Y
authors.initials: RY
authors.lastname: Jia
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: X Y
authors.initials: XY
authors.lastname: Chen
authors.forename: X F
authors.initials: XF
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Bo
authors.forename: D C
authors.initials: DC
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004530:Egg Yolk / Q000276:immunology / Q000821:virology; D004751:Enteritis / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005777:Geese; D007114:Immunization / Q000379:methods / Q000662:veterinary*; D007376:Interleukin-2 / Q000494:pharmacology; D009500:Neutralization Tests / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology*; D011897:Random Allocation; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007376:Interleukin-2; D015164:Vaccines, Inactivated; D014765:Viral Vaccines