title: Proteomic analysis of excretory secretory products from Clonorchis sinensis adult worms: molecular characterization and serological reactivity of a excretory-secretory antigen-fructose-1,6-bisphosphatase.
abstract: Clonorchis sinensis is a food-borne zoonotic parasite that resides in bile ducts and causes clonorchiasis, which may result in cholelithiasis, cholecystitis, hepatic fibrosis, and liver tumors. Although total excretory secretory products (ESP) of C. sinensis adults induce hepatic fibrosis in vivo in rats, the causative mechanism is not well understood. To study components of the ESP, C. sinensis culture medium was collected and analyzed using shotgun LC-MS/MS. We identified a total of 110 proteins, including glycometabolic enzymes (such as fructose-1,6-bisphosphatase (FBPase) and enolase), detoxification enzymes (such as glutamate dehydrogenase, dihydrolipoamide dehydrogenase and cathepsin B endopeptidase), and a number of RAB family proteins. To identify a potential causative agent for hepatic fibrosis, we expressed and purified a recombinant FBPase, a 1,041-bp gene product that encodes a 41.7-kDa protein with prototypical FBPase domains and that can form a tetramer with a molecular mass of 166.8 kDa. In addition, we found that FBPase is an antigen present in the ESP and in circulation. Immunofluorescence showed that FBPase localizes to the intestinal cecum and vitellarium in C. sinensis adults. Our results describe the components of the excretory secretory products from C. sinensis adult worms and suggest that FBPase may be an important antigen present in the ESP of C. sinensis and may lay the foundation for additional studies on the development of clonorchiasis-associated hepatic fibrosis.
authors.affiliation: Department of Parasitology, Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou 510080, People's Republic of China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Zheng
authors.forename: Kunhua
authors.initials: K
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xuchu
authors.initials: X
authors.lastname: Hu
authors.forename: Fengyu
authors.initials: F
authors.lastname: Hu
authors.forename: Lisi
authors.initials: L
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Wenfang
authors.initials: W
authors.lastname: Li
authors.forename: Chi
authors.initials: C
authors.lastname: Liang
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Yin
authors.forename: Qingyu
authors.initials: Q
authors.lastname: He
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
mesh_terms: D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D003004:Clonorchis sinensis / Q000737:chemistry*; D003470:Culture Media / Q000737:chemistry; D006597:Fructose-Bisphosphatase / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D000947:Antigens, Helminth; D003470:Culture Media; D020543:Proteome; D006597:Fructose-Bisphosphatase