title: Metabolic status rather than cell cycle signals control quiescence entry and exit.
abstract: Quiescence is defined as a temporary arrest of proliferation, yet it likely encompasses various cellular situations. Our knowledge about this widespread cellular state remains limited. In particular, little is known about the molecular determinants that orchestrate quiescence establishment and exit. Here we show that upon carbon source exhaustion, budding yeast can enter quiescence from all cell cycle phases. Moreover, using cellular structures that are candidate markers for quiescence, we found that the first steps of quiescence exit can be triggered independently of cell growth and proliferation by the sole addition of glucose in both Saccharomyces cerevisiae and Schizosaccharomyces pombe. Importantly, glucose needs to be internalized and catabolized all the way down to glycolysis to mobilize quiescent cell specific structures, but, strikingly, ATP replenishment is apparently not the key signal. Altogether, these findings strongly suggest that quiescence entry and exit primarily rely on cellular metabolic status and can be uncoupled from the cell cycle.
authors.affiliation: Université de Bordeaux, Institut de Biochimie et Génétique Cellulaires, UMR 5095, F-33000 Bordeaux, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Laporte
authors.forename: Anne
authors.initials: A
authors.lastname: Lebaudy
authors.forename: Annelise
authors.initials: A
authors.lastname: Sahin
authors.forename: Benoît
authors.initials: B
authors.lastname: Pinson
authors.forename: Johanna
authors.initials: J
authors.lastname: Ceschin
authors.forename: Bertrand
authors.initials: B
authors.lastname: Daignan-Fornier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Sagot
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology; D012568:Schizosaccharomyces / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000255:Adenosine Triphosphate; D005947:Glucose