title: Redd1 inhibits the invasiveness of non-small cell lung cancer cells.
abstract: Redd1 acts as a negative regulator of mTOR in response to various stress conditions, but its specific physiological role is currently unclear. In the present study, we showed that Redd1 inhibits the invasive activity of non-small cell lung cancer (NSCLC) cells. Interestingly, expression of Redd1 was extremely low in H1299 cells displaying high invasiveness, compared with that in H460 cells with lower invasive activity. Overexpression of Redd1 inhibited the invasive activity of H1299 cells, while suppression with specific siRNAs enhanced the invasiveness of H460 cells. Knockdown of the mTOR downstream substrate, S6K, resulted in a decrease in the invasive property of H1299 cells. Our results provide preliminary evidence that Redd1 inhibits the invasive activity of NSCLC cells via suppression of the mTOR downstream pathway.
authors.affiliation: Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, Seoul 139-706, Republic of Korea. hyeonok@kirams.re.kr
authors.forename: Hyeon-Ok
authors.initials: HO
authors.lastname: Jin
authors.forename: Sung-Keum
authors.initials: SK
authors.lastname: Seo
authors.forename: Sang-Hyeok
authors.initials: SH
authors.lastname: Woo
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Hong
authors.forename: Jae-Youn
authors.initials: JY
authors.lastname: Yi
authors.forename: Woo-Chul
authors.initials: WC
authors.lastname: Noh
authors.forename: Eun-Kyu
authors.initials: EK
authors.lastname: Kim
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Lee
authors.forename: Seok-Il
authors.initials: SI
authors.lastname: Hong
authors.forename: Tae-Boo
authors.initials: TB
authors.lastname: Choe
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Park
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C469991:DDIT4 protein, human; D014157:Transcription Factors; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases