title: Genotoxicity of platinum in embryos of zebrafish (Danio rerio) and ramshorn snail (Marisa cornuarietis).
abstract: The metal platinum is inter alia used for industrial and medical purposes. Due to its application in automobile catalytic converters and as an anti-cancer drug, Pt enters the aquatic environment via road runoff and hospital sewage and raises concerns about its environmental impact and toxicity to organisms. Therefore, the genotoxicity of Pt at 0, 0.1, 1, 10, 50, 100 and 200μg/l PtCl(2) was tested on two freshwater organisms, zebrafish (Danio rerio) and ramshorn snail (Marisa cornuarietis) using the single cell gel electrophoresis, also called comet assay. PtCl(2) did not show any genotoxicity for D. rerio at the tested concentrations, whereas significantly elevated DNA damage was observed in M. cornuarietis at 1μg/l PtCl(2) and beyond. The results of the study suggest a high sensitivity of M. cornuarietis concerning the genotoxic impact of PtCl(2).
authors.affiliation: Animal Physiological Ecology Department, University of Tübingen, Tübingen, Germany. raphaela.osterauer@gmx.de
authors.forename: Raphaela
authors.initials: R
authors.lastname: Osterauer
authors.forename: Christopher
authors.initials: C
authors.lastname: Fassbender
authors.forename: Thomas
authors.initials: T
authors.lastname: Braunbeck
authors.forename: Heinz-R
authors.initials: HR
authors.lastname: Köhler
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D010984:Platinum / Q000633:toxicity*; D012908:Snails / Q000187:drug effects / Q000235:genetics; D014466:Ultraviolet Rays; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical; D010984:Platinum; D006861:Hydrogen Peroxide