title: Association of mycobacteria in recirculating aquaculture systems and mycobacterial disease in fish.
abstract: Mycobacterium marinum isolates cultivated from tissue containing granulomatous lesions in Florida pompano Trachinotus carolinus and from biofilm samples collected from their tank and water recirculating system had identical (L1 of 11 bands) repetitive-sequence-based polymerase chain reaction (rep-PCR) DNA fingerprints. A second M. marinum clone sharing 4 of 11 rep-PCR bands with the first clone was isolated from some fish tissues but not from system samples. Water samples yielded low numbers of colonies of mycobacteria (0.08-1.3/mL), but high numbers were recovered from biofilms (260-12,000/swab) and filters (63-21,000/ filter). Mycobacterium hemophilum, M. chelonae, M. trivale, M. gastri, and M. gordonae were isolated from system samples alone.
authors.affiliation: Tropical Aquaculture Laboratory, Program in Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, Institute of Food and Agricultural Sciences, University of Florida, 1408 24th Street Southeast, Ruskin, Florida 33570, USA.
authors.forename: Roy P E
authors.initials: RP
authors.lastname: Yanong
authors.forename: Deborah B
authors.initials: DB
authors.lastname: Pouder
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Falkinham
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology*; D009164:Mycobacterium Infections / Q000382:microbiology / Q000662:veterinary*; D019910:Mycobacterium marinum / Q000235:genetics* / Q000302:isolation & purification; D010473:Perciformes; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial