title: Functional patterning of DNA origami by parallel enzymatic modification.
abstract: We demonstrate here a rapid and cost-effective technique for nanoscale patterning of functional molecules on the surface of a DNA origami. The pattern is created enzymatically by transferring a functionalized dideoxynucleotide to the 3'-end of an arbitrary selected set of synthetic DNA oligonucleotides positioned approximately 6 nm apart in a 70 × 100 nm(2) rectangular DNA origami. The modifications, which are performed in a single-tube reaction, provide an origami surface modified with a variety of functional groups including chemical handles, fluorescent dyes, or ligands for subsequent binding of proteins. Efficient labeling and patterning was demonstrated by gel electrophoresis shift assays, reverse-phase HPLC, mass spectrometry, atomic force microscopy (AFM) analysis, and fluorescence measurements. The results show a very high yield of oligonucleotide labeling and incorporation in the DNA origami. This method expands the toolbox for constructing several different modified DNA origami from the same set of staple strands.
authors.affiliation: Danish National Research Foundation: Center for DNA Nanotechnology at Interdisciplinary Nanoscience Center (iNANO),Aarhus University , DK-8000 Aarhus, Denmark.
authors.forename: Kasper
authors.initials: K
authors.lastname: Jahn
authors.forename: Thomas
authors.initials: T
authors.lastname: Tørring
authors.forename: Niels Vinther
authors.initials: NV
authors.lastname: Voigt
authors.forename: Rasmus Schøler
authors.initials: RS
authors.lastname: Sørensen
authors.forename: Anne Louise
authors.initials: AL
authors.lastname: Bank Kodal
authors.forename: Ebbe Sloth
authors.initials: ES
authors.lastname: Andersen
authors.forename: Kurt Vesterager
authors.initials: KV
authors.lastname: Gothelf
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
mesh_terms: D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D009841:Oligonucleotides; D004247:DNA