title: Interaction with DNA and different effect on the nucleus of cancer cells for copper(II) complexes of N-benzyl di(pyridylmethyl)amine.
abstract: Three new copper(II) complexes of N-benzyl di(pyridylmethyl)amine (phdpa) were synthesized and characterized by spectroscopic methods. The interaction between CT-DNA and the complexes was studied by UV and fluorescence titration methods. It was found that the complex [(phdpa)Cu(H(2)O)Ac)](Ac), with the non-planar aromatic heterocyclic ring ligand (phdpa), showed good anticancer properties and could cause the fragmentation of the nucleus, although its interaction with CT-DNA was weaker than that of 1,10-phenanthroline (phen)-based copper(II) complexes. The anticancer activities of copper(II) complexes with phdpa and phen based ligands are correlated to their binding constants with DNA, but phen-based copper(II) complexes did not cause the nucleus fragmentation of HeLa cells. [(phdpa)Cu(H(2)O)Ac)](Ac) can noticeably decrease the oxygen content of a culture solution and of HeLa cells, which make it a new nucleus and oxygen related anticancer copper(II) complex. Information obtained here would be helpful in the design of new antitumor complexes in oxidative therapy.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China. chenqy@ujs.edu.cn
authors.forename: Qiu-Yun
authors.initials: QY
authors.lastname: Chen
authors.forename: Hai-Jian
authors.initials: HJ
authors.lastname: Fu
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Ma
authors.forename: Kai-Di
authors.initials: KD
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
mesh_terms: D000588:Amines / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry* / Q000378:metabolism; D056831:Coordination Complexes / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004273:DNA, Neoplasm / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D008968:Molecular Conformation; D009369:Neoplasms / Q000188:drug therapy; D010101:Oxygen Consumption; D010618:Phenanthrolines / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D000588:Amines; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D004273:DNA, Neoplasm; D008024:Ligands; D010618:Phenanthrolines; D003300:Copper; C025205:1,10-phenanthroline