title: Multiplexed detection of oligonucleotides with biobarcoded gold nanoparticle probes.
abstract: Applications in a variety of fields rely on the high-throughput ultrasensitive and multiplexed detection of oligonucleotides. However, the conventional microarray-based techniques that employ fluorescent dyes are hampered by several limitations; they require target amplification, fluorophore labeling, and complicated instrumentation, while the fluorophore-labeled species themselves exhibit slow binding kinetics, photo-bleaching effects, and overlapping spectral profiles. Among the emerging nanomaterials that are being used to solve these problems, oligonucleotide-gold nanoparticle conjugates (Oligo-AuNPs) have recently been highlighted due to their unique chemical and physical properties. In this chapter, a detection scheme for oligonucleotides that utilize Oligo-AuNPs is evaluated with multiple oligonucleotide targets. This scheme takes advantage of the sharp melting transitions, intense optical properties, catalytic properties, enhanced binding properties, and the programmable assembly/disassembly of Oligo-AuNPs.
authors.affiliation: Department of Materials Science and Engineering, Korea University, Seoul, Republic of Korea. jslee79@korea.ac.kr
authors.forename: Jae-Seung
authors.initials: JS
authors.lastname: Lee
mesh_terms: D001330:Electronic Data Processing; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D006046:Gold