title: One-step homogeneous protein detection based on aptamer probe and fluorescence cross-correlation spectroscopy.
abstract: A new protein assay based on fluorescence cross-correlation spectroscopy (FCCS) and aptamer probe is developed. In this assay, two spectrally distinct fluorophores labeled aptamer probes are used to recognize and detect thrombin through a sandwich reaction. The sandwich complexes are diffused through a confocal detection volume. The cross-correlation signals can be observed only at the presence of the aptamer probes-protein sandwich complexes. Thrombin is selected as a target to validate the assay. The whole detection process can be completed within an hour with low-nanomolar sensitivity and high specificity. The novel aptamer-based FCCS detection offers a simple, rapid and sensitive method for protein analysis in a homogeneous format.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhou
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Tang
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013917:Thrombin / Q000032:analysis*
chemical_list: D015335:Molecular Probes; D013917:Thrombin