title: Bovine milk fat enriched in conjugated linoleic and vaccenic acids attenuates allergic dermatitis in mice.
abstract: BACKGROUND
  Orally administered milk fat enriched in conjugated linoleic acid (CLA) and trans-vaccenic acid (VA) ('enriched milk fat'), produced by supplementing the diet of pasture-fed cows with fish and sunflower oil, has been shown previously to suppress the development of allergic airway disease in mice.
  
  
  OBJECTIVE
  To investigate whether topical or oral application of enriched milk fat and its two major fatty acids cis-9, trans-11 CLA (c9,t11-CLA) and VA inhibit allergic dermatitis in mice.
  
  
  METHODS
  Allergic dermatitis was induced in C57BL/6 mice by epicutaneous sensitization of tape-stripped skin with ovalbumin (OVA). Enriched milk fat and its two major fatty acids were either topically applied to the OVA-sensitized skin, or orally fed to mice by supplementation of the diet. Blood and skin tissues were collected for analysis after the third skin sensitization.
  
  
  RESULTS
  Both topical and oral administration of enriched milk fat and its two major fatty acids led to significant suppression of allergic dermatitis as evidenced by reduced clinical and histological scores of affected skins, infiltration of inflammatory cells, and circulating allergen-specific IgE levels, compared with treatment with normal milk fat or the base control diet. C9,t11-CLA and VA individually inhibited multiple facets of allergic dermatitis when topically applied, and their combination produced a strong additive effect.
  
  
  CONCLUSIONS
  Enriched milk fat, and its two major fatty acids c9,t11-CLA and vaccenic acid attenuate allergic dermatitis in mice.
authors.affiliation: LactoPharma Consortium, University of Auckland, Auckland, New Zealand.
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: A K H
authors.initials: AK
authors.lastname: Macgibbon
authors.forename: P
authors.initials: P
authors.lastname: Black
authors.forename: G W
authors.initials: GW
authors.lastname: Krissansen
mesh_terms: D000818:Animals; D002417:Cattle; D017449:Dermatitis, Allergic Contact / Q000178:diet therapy*; D019587:Dietary Supplements; D005223:Fats / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use; D005260:Female; D008041:Linoleic Acids / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000737:chemistry*; D009829:Oleic Acids / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D010047:Ovalbumin; D012882:Skin Tests
chemical_list: D005223:Fats; D008041:Linoleic Acids; D009829:Oleic Acids; D010047:Ovalbumin; C050413:11-octadecenoic acid