title: Catabolism of L-ascorbate by Lactobacillus rhamnosus GG.
abstract: Catabolism of L-ascorbate by enteric bacteria is well documented, but no study has formally proved that bacteria of the Lactobacillus genus ferment this compound. However, some genes analogous to those of yiaK-S operon and ula regulon, which encoded proteins leading to L-ascorbate degradation by Escherichia coli and Klebsiella pneumoniae , have been identified in the recently sequenced Lactobacillus rhamnosus GG genome. Investigations by HPLC and in vivo (13)C NMR using L-[1,6-(13)C]-ascorbate showed that L. rhamnosus GG, a common probiotic strain, has the ability to catabolize L-ascorbate under anaerobiosis. The main products of the ascorbate degradation have been identified as CO(2), acetate, and lactate. These results are in accordance with the metabolic pathway proposed for the fermentation of L-ascorbate by E. coli.
authors.affiliation: Clermont Université, Université Blaise Pascal, Laboratoire de Génie Chimique et Biochimique, Polytech' Clermont Ferrand, Aubière, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Linares
authors.forename: Philippe
authors.initials: P
authors.lastname: Michaud
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Delort
authors.forename: Mounir
authors.initials: M
authors.lastname: Traïkia
authors.forename: Jerome
authors.initials: J
authors.lastname: Warrand
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D001205:Ascorbic Acid