title: Calpain mediates degradation of cytoskeletal proteins during Jurkat T-cell death induced by Entamoeba histolytica.
abstract: Entamoeba histolytica is known to induce host cell death via activation of calpain and caspases. In this study, we investigated the specific proteases involved in the degradation of cytoskeletal proteins during Jurkat T-cell death induced by E. histolytica. Amoebic trophozoites induced marked degradation of paxillin, Cas, vimentin, vinculin and talin, as well as α- or β-spectrin, in Jurkat T cells. The cleavage effects of E. histolytica were strongly retarded by pretreatment with a calpain inhibitor, but not with a pan-caspase inhibitor. In addition, calpain knockdown with siRNA in Jurkat T cells effectively inhibited E. histolytica-induced PARP, paxillin, α-spectrin, β-spectrin and talin degradation, as compared to scrambled siRNA. These results suggest that calpain plays a crucial role in the cleavage of cytoskeletal proteins during cell death induced by E. histolytica.
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Y A
authors.initials: YA
authors.lastname: Lee
authors.forename: K A
authors.initials: KA
authors.lastname: Kim
authors.forename: M H
authors.initials: MH
authors.lastname: Shin
mesh_terms: D002154:Calpain / Q000378:metabolism*; D016923:Cell Death; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000276:immunology* / Q000472:pathogenicity*; D006801:Humans; D019169:Jurkat Cells / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; D002154:Calpain