title: Inhibitory constituents of Euonymus alatus leaves and twigs on nitric oxide production in BV2 microglia cells.
abstract: The excessive and prolonged nitric oxide (NO) production has been linked to various inflammatory diseases as well as tumourigenesis. On the search for anti-inflammatory and anti-cancer compounds from the medicinal plants, the methanolic extract of Euonymus alatus (Thunb.) Sieb. (Celastraceae) was found to have significant inhibitory activity on NO production in lipopolysaccharide (LPS)-stimulated BV2 microglia cells. Hence, we attempted to isolate the inhibitory constituent of E. alatus leaves and twigs on NO production. Thirteen compounds including two new glycerol derivates (1, 2), two C(13) isoprenoids (3, 4), two phenolics (5, 6) and seven flavonoids (7-13) were isolated, and the structures of 1-13 were elucidated by extensive 1D and 2D spectroscopic methods. The isolated compounds significantly inhibited NO production induced by LPS in BV2 microglia cells.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Heejung
authors.initials: H
authors.lastname: Yang
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: So Young
authors.initials: SY
authors.lastname: Kang
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D029756:Euonymus / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide