title: Multi-spectroscopic method study the interaction of anti-inflammatory drug ketoprofen and calf thymus DNA and its analytical application.
abstract: Interactions of the anti-inflammatory drug ketoprofen with calf thymus DNA (ctDNA) in aqueous solution have been studied by multi-spectroscopic method including resonance light scattering (RLS) technique, ultraviolet spectra (UV), (1)H NMR, etc. The characteristics of RLS spectra, the effective factors and optimum conditions of the reaction have been unequivocally investigated. Mechanism investigations have shown that ketoprofen can bind to ctDNA by groove binding and form large particles, which resulted in the enhancement of RLS intensity. In Critic acid-Na(2)HPO(4) buffer (pH=6.5), ketoprofen has a maximum peak 451.5 nm and the RLS intensity is remarkably enhanced by trace amount of ctDNA due to the interaction between ketoprofen and ctDNA. The enhancement of RLS signal is directly proportional to the concentration of ctDNA in the range of 1.20×10(-6)-1.0×10(-5) mol/L, and its detection limit (3σ) is 1.33×10(-9) mol/L. The method is simple, rapid, practical and relatively free from interference generated by coexisting substance, and was applied to the determination of trace amounts of nucleic acid in synthetic samples with satisfactory results.
authors.affiliation: Department of Chemistry, Key Laboratory of Environment-Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan, PR China.
authors.forename: Hongqin
authors.initials: H
authors.lastname: Guo
authors.forename: Changqun
authors.initials: C
authors.lastname: Cai
authors.forename: Hang
authors.initials: H
authors.lastname: Gong
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
mesh_terms: D000042:Absorption; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D002021:Buffers; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007660:Ketoprofen / Q000737:chemistry*; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D009691:Nucleic Acid Denaturation; D012542:Scattering, Radiation; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis / Q000379:methods*; D013997:Time Factors; D014783:Viscosity
chemical_list: D000893:Anti-Inflammatory Agents; D002021:Buffers; D012996:Solutions; D004247:DNA; D007660:Ketoprofen; C427203:calf thymus DNA