title: [SNP typing of formalin-fixed tissue in individual identification].
abstract: OBJECTIVE
  To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome.
  
  
  METHODS
  Genomic DNA was prepared from formalin-fixed tissue. In the event that typing using Sinofiler and MiniFiler kits had failed, 51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). RESULTS Full profiles of X-SNP loci were obtained from the formalin-fixed tissue, while the STR and miniSTR genotyping failed.
  
  
  CONCLUSIONS
  SNP genotyping technique can be used to obtain more information for formalin fixed tissues.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. liny@ssfjd.cn
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhen-min
authors.initials: ZM
authors.lastname: Zhao
mesh_terms: D041321:Chromosomes, Human, X; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers; D005260:Female; D053803:Forensic Genetics; D005557:Formaldehyde; D005838:Genotype; D006801:Humans; D007422:Intestines; D060885:Multiplex Polymerase Chain Reaction; D016612:Paraffin Embedding; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D017931:DNA Primers; D005557:Formaldehyde; D004247:DNA