title: Stromal cells directly mediate the re-establishment of the lymph node compartments after transplantation by CXCR5 or CCL19/21 signalling.
abstract: Lymph nodes (LN) are highly organized and have characteristic compartments. Destruction of these compartments leads to an inability to fulfil their immunological function. However, it is not yet clearly understood which mechanisms are involved in the development and maintenance of this organization. After transplantation of LN into the mesentery, the LN regenerate to fully functional LN. In this study, the question was addressed, how stromal cells in the B-cell follicles (follicular dendritic cells), which were identified by CD21/CD35, and stromal cells in the T-cell area (gp38+ cells) are involved via chemokine signalling. The gp38+ cells and CD21/CD35+ cells were detected in the transplanted LN (EGFP, plt/plt and CXCR5(-/-) mice) over a period of 8 weeks to analyse their competence to reconstruct the compartmental organization. The presence of gp38+ cells was stable during regeneration and these cells reconstructed the T-cell area within 4 weeks. After transplantation of plt/plt LN CCL19/CCL21 expression was observed leading to partial restoration of the T-cell area. In contrast, there were changes in the presence and morphology of CD21/CD35+ cells within the B-cell area during reconstruction, which was dependent on the presence of B cells and CXCL13/CXCR5 signalling. Hence, CD21/CD35+ cells and gp38+ cells are involved in the establishment of the compartmental organization of lymph nodes but using different ways to recruit lymphocytes via chemokine signalling.
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Buettner
authors.forename: Ulrike
authors.initials: U
authors.lastname: Bode
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D054415:Chemokine CCL19 / Q000276:immunology*; D054421:Chemokine CCL21 / Q000276:immunology*; D005260:Female; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D054380:Receptors, CXCR5; D017463:Receptors, Complement 3b / Q000276:immunology; D015398:Signal Transduction; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D000098853:Podoplanin
chemical_list: D054415:Chemokine CCL19; D054421:Chemokine CCL21; D008562:Membrane Glycoproteins; D054380:Receptors, CXCR5; D017463:Receptors, Complement 3b; C490441:Pdpn protein, mouse; D000098853:Podoplanin