title: A limited number of ITS haplotypes defines the diversity of Pneumocystis jirovecii strains in Sweden.
abstract: The infectious fungus Pneumocystis jirovecii remains an important cause of pneumonia (PCP) in the immunocompromised host. To study the biodiversity of P. jirovecii in Sweden the internal transcribed spacers (ITS) of the rDNA locus were amplified, cloned and sequenced from a set of diagnostic respiratory specimens obtained from 64 patients with P. jirovecii infection during the years 1996-2003. The analysis of 408 cloned sequences from amplified products resulted in the identification of 10 ITS1 and 12 ITS2 established genotypes. Twelve ITS haplotypes (combinations of ITS1 and ITS2) were identified of which nine were found to recur during the time span of the study. Haplotype Eg was the most common, followed by Ne, Bi and Eb. A new ITS2 genotype denoted v was identified in specimens from four patients. There was no association between ITS haplotype and patient age, sex, underlying disease or geographical origin. Shannon and Simpson index analysis revealed no difference in diversity in Sweden compared to other countries studied and no changes in diversity were found during the study period in Sweden. Criteria were defined to enable the discrimination of genuine ITS types and a more accurate assessment of the previously overestimated genetic diversity of P. jirovecii populations. A model depicting the phylogenetic and genealogic relationships in a revised set of global types of this fungus is presented.
authors.affiliation: Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden. Jessica.Beser@smi.se
authors.forename: Jessica
authors.initials: J
authors.lastname: Beser
authors.forename: Silvia
authors.initials: S
authors.lastname: Botero-Kleiven
authors.forename: Marianne
authors.initials: M
authors.lastname: Lebbad
authors.forename: Per
authors.initials: P
authors.lastname: Hagblom
authors.forename: Victor
authors.initials: V
authors.lastname: Fernandez
mesh_terms: D021901:DNA, Intergenic / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D010802:Phylogeny; D045363:Pneumocystis carinii / Q000235:genetics*; D011020:Pneumonia, Pneumocystis / Q000453:epidemiology / Q000382:microbiology*; D013548:Sweden / Q000453:epidemiology
chemical_list: D021901:DNA, Intergenic