title: Peptide nanofibers preconditioned with stem cell secretome are renoprotective.
abstract: Stem cells may contribute to renal recovery following acute kidney injury, and this may occur through their secretion of cytokines, chemokines, and growth factors. Here, we developed an acellular, nanofiber-based preparation of self-assembled peptides to deliver the secretome of embryonic stem cells (ESCs). Using an integrated in vitro and in vivo approach, we found that nanofibers preconditioned with ESCs could reverse cell hyperpermeability and apoptosis in vitro and protect against lipopolysaccharide-induced acute kidney injury in vivo. The renoprotective effect of preconditioned nanofibers associated with an attenuation of Rho kinase activation. We also observed that the combined presence of follistatin, adiponectin, and secretory leukoprotease during preconditioning was essential to the renoprotective properties of the nanofibers. In summary, we developed a designer-peptide nanofiber that can serve as a delivery platform for the beneficial effects of stem cells without the problems of teratoma formation or limited cell engraftment and viability.
authors.affiliation: Department of Medicine/Nephrology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Erica
authors.initials: E
authors.lastname: Bakota
authors.forename: Benny H J
authors.initials: BH
authors.lastname: Chang
authors.forename: Mark
authors.initials: M
authors.lastname: Entman
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Hartgerink
authors.forename: Farhad R
authors.initials: FR
authors.lastname: Danesh
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D064987:Cell- and Tissue-Based Therapy / Q000009:adverse effects / Q000379:methods*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000637:transplantation*; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D057139:Nanofibers / Q000627:therapeutic use; D011507:Proteinuria / Q000517:prevention & control; D054460:rho-Associated Kinases / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D054460:rho-Associated Kinases