title: A set of P-element transformation vectors permitting the simplified generation of fluorescent fusion proteins in Drosophila melanogaster.
abstract: We have assembled a molecular toolkit enabling the facile generation of C-terminal fluorescent protein fusion constructs for the construction of transgenic Drosophila lines in the form of a series of modified vectors for P-element transformation. These vectors contain one of a variety of fluorescent tags including GFP, mCherry, Venus, Cerulean, photoactivatable paGFP and the red-to-green photoconvertible protein Dendra and provide a range of options with respect to transcriptional regulation. The vectors have been extensively tested in vivo and can produce fluorescent chimeric proteins that are functional.
authors.affiliation: Department of Molecular Biology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Viktor Karlovitch
authors.initials: VK
authors.lastname: Lund
authors.forename: Yvonne
authors.initials: Y
authors.lastname: DeLotto
authors.forename: Robert
authors.initials: R
authors.lastname: DeLotto
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D008164:Luminescent Proteins; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins