title: Acellular dermal matrix seeded with adipose-derived stem cells as a subcutaneous implant.
abstract: BACKGROUND
  Soft tissue augmentation using autologous materials usually is associated with high resorption rates. To obtain more durable soft tissue filler, acellular dermal matrix (ADM) was seeded with adipose-derived stem cells (ASCs) in this study.
  
  
  METHODS
  For ADM preparation, split-thickness skin was obtained from the dorsum of two Fischer rats. Harvested skin was acellularized to obtain ADM and subsequently seeded in vitro with ASCs isolated from the same rats. Subcutaneous soft tissue augmentation was carried out in the dorsal area of 20 Fischer rats. The implant materials were ADM (group 1), ADM with ASCs (group 2), collagen type 1 gel (group 3), and collagen type 1 gel with ASCs (group 4). Each specimen was harvested after 8 weeks for quantitative evaluation of thickness, vascular density, and collagen content.
  
  
  RESULTS
  Histologic analysis showed that ASCs were successfully seeded onto ADM. The thickness of the implanted material and the vascular density were highest 8 weeks postoperatively in group 2. The subpanniculus layer became significantly thicker in group 3 than in group 4. The collagen content was significantly higher in group 2 than in the other groups.
  
  
  CONCLUSIONS
  These findings suggest that ADM seeded with ASCs forms the best implant material in terms of volume maintenance, vascular density, and collagen content.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan. hakanorbay@nms.ac.jp
authors.forename: Hakan
authors.initials: H
authors.lastname: Orbay
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takami
authors.forename: Hiko
authors.initials: H
authors.lastname: Hyakusoku
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D004195:Disease Models, Animal; D006084:Graft Rejection; D006085:Graft Survival; D007150:Immunohistochemistry; D008297:Male; D019736:Prostheses and Implants; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D012680:Sensitivity and Specificity; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D033581:Stem Cell Transplantation; D023822:Tissue Engineering