title: Analysis of protein S-nitrosylation.
abstract: S-Nitrosylation, the redox-based modification of cysteine thiol side chains by nitric oxide, is a dynamic and reversible post-translational modification of proteins that subserves many important cellular functions. Analysis of protein S-nitrosylation is often challenging due to methodological limitations and the effects of various chemical and physical parameters. Despite these technical challenges, a growing number of useful methods are now available to analyze protein S-nitrosylation. In this unit, several important methods to measure protein S-nitrosylation and denitrosylation are discussed and evaluated. Recommendations are given regarding the potential and the applicability of the methods discussed.
authors.affiliation: Tufts Cummings School of Veterinary Medicine, North Grafton, Massachusetts.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Schonhoff
authors.affiliation: Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Moran
authors.initials: M
authors.lastname: Benhar
mesh_terms: D001671:Biochemistry / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D026403:S-Nitrosothiols / Q000032:analysis* / Q000378:metabolism
chemical_list: D011506:Proteins; D026403:S-Nitrosothiols; D009569:Nitric Oxide