title: Secretion-and-capture cell-surface display for selection of target-binding proteins.
abstract: This report describes a new cell-surface display system, the Secretion and Capture Technology (SECANT™) platform, which relies on in vivo biotinylation of the protein of interest followed by its capture on the avidinated surface of the parent cell. Cell sorting techniques are then used to isolate clones that display target-binding protein. A distinguishing feature of this method is its ability to display complex proteins, such as full-length immunoglobulin G (IgG) antibodies, on living cells. In this proof-of-concept study, Saccharomyces cerevisiae cells that displayed Herceptin IgG were isolated from a 10,000-fold excess of cells that displayed a lysozyme-binding antibody.
authors.affiliation: Celexion LLC, One Kendall Square, Suite 14302, Cambridge, MA 02139, USA. arakestraw@celexionbio.com
authors.forename: J A
authors.initials: JA
authors.lastname: Rakestraw
authors.forename: D
authors.initials: D
authors.lastname: Aird
authors.forename: P M
authors.initials: PM
authors.lastname: Aha
authors.forename: B M
authors.initials: BM
authors.lastname: Baynes
authors.forename: D
authors.initials: D
authors.lastname: Lipovsek
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000378:metabolism; D000915:Antibody Affinity; D001360:Avidin; D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D005434:Flow Cytometry; D005822:Genetic Vectors; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D007163:Immunosorbent Techniques; D009842:Oligopeptides; D010455:Peptides; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009842:Oligopeptides; D010455:Peptides; D011506:Proteins; D001360:Avidin; C085706:FLAG peptide