title: Molecular characterization, expression and chromosomal localization of porcine PNPLA3 and PNPLA4.
abstract: Patatin-like phospholipases (PNPLAs) comprise a protein family whose members contain a conserved patatin-like domain with lipid acyl hydrolase activity. The cloning and characterization of full-length cDNAs of PNPLA3 and PNPLA4 in pigs is reported and, for the first time, an alternative splicing variant of porcine PNPLA4, which skips exon 5 of the normal transcript, was identified. Subsequently, tissue expression analysis by quantitative PCR indicated that porcine PNPLA3 was mainly expressed in both adipose and liver whereas porcine PNPLA4 was abundant in liver. Porcine PNPLA3 and PNPLA4 were assigned respectively to chromosome 5p11-p15 and Xp24.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: An
authors.initials: A
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Lei
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Long
authors.forename: Zaiqing
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015870:Gene Expression; D020869:Gene Expression Profiling; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D007527:Isoenzymes; D010740:Phospholipases