title: Marked induction of c-Maf protein during Th17 cell differentiation and its implication in memory Th cell development.
abstract: Until recently, effector T helper (Th) cells have been classified into two subsets, Th1 and Th2 cells. Since the discovery of Th17 cells, which produce IL-17, much attention has been given to Th17 cells, mainly because they have been implicated in the pathogenesis of various inflammatory diseases. We have performed transcriptome analysis combined with factor analysis and revealed that the expression level of c-Maf, which is considered to be important for Th2 differentiation, increases significantly during the course of Th17 differentiation. The IL-23 receptor (IL-23R), which is important for Th17 cells, is among putative transcriptional targets of c-Maf. Interestingly, the analysis of c-Maf transgenic Th cells revealed that the overexpression of c-Maf did not lead to the acceleration of the early stage of Th17 differentiation but rather to the expansion of memory phenotype cells, particularly with Th1 and Th17 traits. Consistently, mouse wild-type memory Th cells expressed higher mRNA levels of c-Maf, IL-23R, IL-17, and IFN-γ than control cells; in contrast, Maf(-/-) memory Th cells expressed lower mRNA levels of those molecules. Thus, we propose that c-Maf is important for the development of memory Th cells, particularly memory Th17 cells and Th1 cells.
authors.affiliation: Department of Rheumatology and Applied Immunology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan. satok@saitama-med.ac.jp
authors.forename: Kojiro
authors.initials: K
authors.lastname: Sato
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Miyoshi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yokota
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Araki
authors.forename: Yu
authors.initials: Y
authors.lastname: Asanuma
authors.forename: Yuji
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Keigyou
authors.initials: K
authors.lastname: Yoh
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aburatani
authors.forename: Toshihide
authors.initials: T
authors.lastname: Mimura
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000276:immunology*; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D007156:Immunologic Memory / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics* / Q000276:immunology / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000166:cytology*; D015533:Transcriptional Activation / Q000276:immunology*
chemical_list: C495608:Maf protein, mouse; D051257:Proto-Oncogene Proteins c-maf; D012333:RNA, Messenger