title: [Expression and clinical significance of Toll-like receptors in human renal carcinoma cell 786-0 and normal renal cell HK-2].
abstract: OBJECTIVE
  To investigate the expression of toll-like receptors (TLR1-TLR10) in human renal carcinoma cell 786-0 and normal renal cell HK-2 and discuss the significance of TLRs in the development of renal carcinoma.
  
  
  METHODS
  Human renal carcinoma cell 786-0 (experimental group) and normal renal cell HK-2 (control group) were cultured. The expression of TLR1-TLR10 was detected by real-time fluorogenic quantitative PCR (FQ-PCR).
  
  
  RESULTS
  The FQ-PCR analysis demonstrated that 786-0 cell line and HK-2 cell line expressed TLR1-TLR6 and TLR8-TLR10 mRNA. Yet neither had an expression of TLR7. Moreover, the expression levels of TLR1, TLR2, TLR4, TLR5, TLR6, TLR8 and TLR10 in the experimental group were significantly higher than those in HK-2 cell line [(4.40 ± 0.19) × 10(-2), (1.77 ± 0.24) × 10(-2), (5.03 ± 0.62) × 10(-2), (3.93 ± 0.86) × 10(-2), (6.11 ± 0.48) × 10(-2), (1.89 ± 0.47) × 10(-2), (2.84 ± 0.57) × 10(-2) vs (0.84 ± 0.27) × 10(-2), (0.40 ± 0.04) × 10(-2), (0.95 ± 0.73) × 10(-2), (0.56 ± 0.25) × 10(-2), (0.49 ± 0.16) × 10(-2), (0.20 ± 0.07) × 10(-2), (0.27 ± 0.08) × 10(-2), P < 0.01]. There was no significant change in the expressions of TLR3 and TLR9 between 786-0 and HK-2 cell line (P > 0.05).
  
  
  CONCLUSIONS
  The expressions of TLR1, TLR2, TLR4, TLR5, TLR6, TLR8 and TLR10 are significantly higher in 786-0 cell line than those in HK-2 cell line. They may play key roles in the development of renal carcinoma.
authors.affiliation: Department of Laboratory Medicine, Affiliated Tenth People's Hospital, Tongji University, Shanghai 200072, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yu
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Xu
authors.forename: Qin-Quan
authors.initials: QQ
authors.lastname: Cheng
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
mesh_terms: D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D007668:Kidney / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051193:Toll-Like Receptors