title: Systematic antibody and antigen-based proteomic profiling with microarrays.
abstract: Current approaches within affinity-based proteomics are driven both by the accessibility and availability of antigens and capture reagents, and by suitable multiplexed technologies onto which these are implemented. By combining planar microarrays and other multiparallel systems with sets of reagents, possibilities to discover new and unpredicted protein-disease associations, either via directed hypothesis-driven or via undirected hypothesis-generating target selection, can be created. In the following stages, the discoveries made during these screening phases have to be verified for potential clinical relevance based on both technical and biological aspects. The use of affinity tools throughout discovery and verification has the potential to streamline the introduction of new markers, as transition into clinically required assay formats appears straightforward. In this article, we summarize some of the current building blocks within array- and affinity-based proteomic profiling with a focus on body fluids, by giving a perspective on how current and upcoming developments in this bioscience could enable a path of pursuit for biomarker discovery.
authors.affiliation: Science for Life Laboratory, Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Burcu
authors.initials: B
authors.lastname: Ayoglu
authors.forename: Anna
authors.initials: A
authors.lastname: Häggmark
authors.forename: Maja
authors.initials: M
authors.lastname: Neiman
authors.forename: Ulrika
authors.initials: U
authors.lastname: Igel
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.forename: Jochen M
authors.initials: JM
authors.lastname: Schwenk
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
mesh_terms: D000906:Antibodies; D000941:Antigens; D015415:Biomarkers; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens; D015415:Biomarkers; D020543:Proteome