title: Highly selective needle-type glucose sensors prepared by the immobilization of glucose oxidase on γ-polyglutamic acid film.
abstract: Fine needle-type glucose sensors with an outer diameter of less that 0.2 mm were fabricated using a low-cost biodegradable γ-polyglutamic acid (PGA) not only as a glucose oxidase (GOx) immobilizing material, but also as a permselective inner membrane. PGA film was prepared on a cellulose acetate-coated Pt-Ir wire electrode, and GOx was covalently immobilized on its surface using water-soluble carbodiimide. The obtained electrode was practically not affected by the existence of electroactive compounds, and provided long-term stability for approximately one month. It also functioned in horse serum with a good linear relationship between the current and glucose concentrations within the range of 2.8 to 22.4 mM.
authors.affiliation: Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima, Tokushima, Japan. mik@chem.tokushima-u.ac.jp
authors.forename: Mikito
authors.initials: M
authors.lastname: Yasuzawa
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Edagawa
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Hiroki
authors.initials: H
authors.lastname: Takaoka
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yabutani
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006736:Horses; D008567:Membranes, Artificial; D009339:Needles; D011099:Polyglutamic Acid / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D001786:Blood Glucose; D004129:Dimethylpolysiloxanes; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011140:Polyurethanes; D011099:Polyglutamic Acid; C005062:acetylcellulose; C013830:baysilon; D002482:Cellulose; D005949:Glucose Oxidase; D005947:Glucose