title: Maintenance of long-term immunological memory by Ig+CD45R+ non-plasma B cells following mucosal immunizations.
abstract: To determine whether long-term immunological B cell memory following mucosal vaccinations is maintained by terminally differentiated Ig-CD45R- plasma cells or Ig+CD45R+ B cells, we immunized mice orally with the non-toxic B subunit of cholera toxin (CTB) as a carrier protein haptenated with FITC (CTB-FITC) plus CT adjuvant. We found that the adoptive transfer of Ig+CD45R+ but not the Ig-CD45R- cells, resulted in higher numbers of FITC-specific IgA-secreting cells in the intestine as well as higher anti-FITC serum IgA titers, suggesting that long term B cell immunological memory following oral vaccinations preferentially resided within the Ig+CD45R+ B cell population.
authors.affiliation: Novartis Vaccines and Diagnostics Inc., Cambridge, MA, USA.
authors.forename: Mingke
authors.initials: M
authors.lastname: Yu
authors.forename: Amanda
authors.initials: A
authors.lastname: Goodsell
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhou
authors.forename: Michael
authors.initials: M
authors.lastname: Vajdy
mesh_terms: D000284:Administration, Oral; D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006241:Haptens / Q000276:immunology; D018928:Immunity, Mucosal / Q000276:immunology*; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000276:immunology
chemical_list: D000939:Epitopes; D006241:Haptens; D007070:Immunoglobulin A