title: Dual-fluorophore ratiometric pH nanosensor with tuneable pKa and extended dynamic range.
abstract: Ratiometric pH nanosensors with tuneable pK(a) were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green(®) dextran (OG-D)) and a reference fluorophore (5-(and-6)-carboxytetramethylrhodamine dextran (TAMRA-D)), in a biocompatible polymer matrix. Dual-fluorophore pH nanosensors permit the measurement of an extended dynamic range, from pH 4.0 to 7.5.
authors.affiliation: Laboratory of Biophysics and Surface Analysis, School of Pharmacy, Boots Sciences Building, University Park, Nottingham, UK NG7 2RD.
authors.forename: Veeren M
authors.initials: VM
authors.lastname: Chauhan
authors.forename: Gary R
authors.initials: GR
authors.lastname: Burnett
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Aylott
mesh_terms: D001672:Biocompatible Materials / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D003911:Dextrans / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007476:Ionophores; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D005456:Fluorescent Dyes; D007476:Ionophores; D011108:Polymers; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate