title: [Functional study of transcription factor Hlx modified dendritic cell line DC2.4].
abstract: OBJECTIVE
  To transfect Hlx into mouse dendritic cell line DC2.4 and observe the effect of hlx on function of dendritic cells.
  
  
  METHODS
  The eukaryotic expression vector PIRES2-EGFP/Hlx was transfected into DC2.4 by liposomes. The transfection efficiency was identified through FACS. RT-PCR and Real-time PCR were used to test the transcription level of Hlx in DC2.4. Forty-eight hours after transfection, DC2.4 cells were studied for cytokine production, cell phenotype, phagocytosis, unilateral mixed lymphocyte reaction.
  
  
  RESULTS
  The pIRES2-EGFP/Hlx vector was transfected into DC2.4 with the transfection efficiency of up to 60%. Highly expressed Hlx in DC2.4 increased the expression of maturation makers including CD80 and CD86, and major histocompatibility complex-II. Functional assay showed that over-expression of Hlx in DC2.4 increased the interleukin-12 transcription and decreased DC endocytosis. The Hlx modified DC2.4 highly expressed IL-10 and TGF-β at the same time. Furthermore, it was shown that in a unilateral mixed lymphocyte reaction model, Hlx modified DC2.4 inhibited proliferation of lymphocytes.
  
  
  CONCLUSIONS
  Transient over-expression of Hlx in DC2.4 promotes DC2.4 maturation and up-regulates IL-12, IL-10 and TGF-β expression. However, the Hlx modified DC2.4 cells functionally appear as regulatory dendritic cells.
authors.affiliation: Zhenjiang Entry-exit Inspection and Quarantine Bureau, Zhenjiang 212008, China. zjsyzcl@126.com
authors.forename: Cheng-lin
authors.initials: CL
authors.lastname: Zhou
authors.forename: Zhao-liang
authors.initials: ZL
authors.lastname: Su
authors.forename: Jin-lian
authors.initials: JL
authors.lastname: Zhu
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: He
authors.forename: Sheng-jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Hua-xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004705:Endocytosis; D006683:HLA-DQ Antigens / Q000378:metabolism; D059866:HLA-DQ beta-Chains; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D016207:Cytokines; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; C489999:Hlx protein, mouse; D018398:Homeodomain Proteins; D008562:Membrane Glycoproteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D018664:Interleukin-12