title: Gene expression changes in Arabidopsis seedlings during short- to long-term exposure to 3-D clinorotation.
abstract: Seedlings of Arabidopsis thaliana (cv. Columbia) were used to evaluate dynamic transcriptional-level genome responses to simulated microgravity condition created by 3-D clinorotation. The DNA chip data analysis showed that the plant may respond to simulated microgravity by dynamic induction (up- and down-regulations) of the responsive genes in the genome. The qRT-PCR results on the investigated genes showed that the expression patterns of the genes (molecular response) were generally similar to the physiological response patterns detected in stress-challenged plants. Expression patterns were categorized into short or continual up- or down-regulated patterns, as well as stochastic changes from short- to long-term simulated microgravity stress. The induced genes are then assumed to establish a new molecular plasticity to the newly adjusted genome status in the basic milieu of maintaining homeostasis during the process of adaptation to simulated microgravity.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Soh
authors.forename: Chungkyun
authors.initials: C
authors.lastname: Auh
authors.forename: Woong-Young
authors.initials: WY
authors.lastname: Soh
authors.forename: Kyeongsik
authors.initials: K
authors.lastname: Han
authors.forename: Donggiun
authors.initials: D
authors.lastname: Kim
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
authors.forename: Yong
authors.initials: Y
authors.lastname: Rhee
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D012399:Rotation; D036226:Seedlings / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological / Q000235:genetics; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics; D018474:Weightlessness Simulation / Q000379:methods*
chemical_list: D012333:RNA, Messenger; D018749:RNA, Plant