title: The deubiquitylating enzyme Cops6 regulates different developmental processes during early zebrafish embryogenesis.
abstract: Zebrafish cops6 encodes a putative deubiquitylating enzyme (DUB) that belongs to the JAMM family. It consists of 297 amino acids and includes the Mov34/MPN/PAD-1 (PF01398) domain. Ubiquitylation is involved in many cellular processes and deconjugation of ubiquitin-modified substrates is important to maintain a sufficient amount of free ubiquitin in the cell. Here, we report our findings regarding the general function of the cops6 gene, as a continuation of our previous studies involving DUB knockdown screening. We have found that cops6 plays different roles in early embryonic development in the zebrafish, including dorsoventral patterning, convergent extension movement and brain formation. In addition, our findings indicate that cops6 plays an anti-apoptotic role during segmentation. Overall, the present study that consolidates our previous work on zebrafish DUB genes, corroborates the hypothesis of multi-functional roles for DUB genes during development.
authors.affiliation: Laboratory of Developmental Signalling and Patterning, Genes and Development Division, Institute of Molecular and Cell Biology, Singapore.
authors.forename: William K F
authors.initials: WK
authors.lastname: Tse
authors.forename: May-Su
authors.initials: MS
authors.lastname: You
authors.forename: Steven Hao-Kee
authors.initials: SH
authors.lastname: Ho
authors.forename: Yun-Jin
authors.initials: YJ
authors.lastname: Jiang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D019521:Body Patterning / Q000235:genetics; D001921:Brain / Q000196:embryology / Q000378:metabolism; D000075686:COP9 Signalosome Complex; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D010450:Endopeptidases / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C559820:Cops6 protein, zebrafish; D029961:Zebrafish Proteins; D010450:Endopeptidases; D000075686:COP9 Signalosome Complex