title: The role of neutrophils during mild and severe influenza virus infections of mice.
abstract: Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections.
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia.
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Tate
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Ioannidis
authors.forename: Ben
authors.initials: B
authors.lastname: Croker
authors.forename: Lorena E
authors.initials: LE
authors.lastname: Brown
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Brooks
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Reading
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D009975:Orthomyxoviridae / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology*; D012137:Respiratory System / Q000187:drug effects / Q000276:immunology / Q000821:virology; D014774:Virulence / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal