title: Chemical composition and antioxidant activity of the essential oil and various extracts of Haplophyllum robustum Bge.
abstract: This study was designed to examine the chemical composition and in vitro antioxidant activity of the essential oil and various extracts of Haplophyllum robustum. GC-MS analysis of the oil resulted in the identification of 30 compounds, representing 99.2% of the oil; 1,8-cineole (38.1%), myrcene (10.7%), α-pinene (8.5%), 4-terpineol (7.0%) and sabinene (6.1%) were the main components. The antioxidant potential of the oil and extracts was evaluated using three separate methods: inhibition of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene-linoleic acid assay and reducing power systems. The essential oil of the plant was able to reduce the stable free radical DPPH with an IC(50) of 72.0 ± 1.2 µg mL(-1) (the IC(50) for ascorbic acid was determined as 5.8 ± 0.4 µg mL(-1)). The essential oil also showed strong reducing power. The level of total phenolics was highest in the essential oil (179.5 ± 2.1 µg mg(-1)).
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. mbgholivand@yahoo.com
authors.forename: M B
authors.initials: MB
authors.lastname: Gholivand
authors.forename: M
authors.initials: M
authors.lastname: Rahimi-Nasrabadi
authors.forename: H
authors.initials: H
authors.lastname: Batooli
authors.forename: H
authors.initials: H
authors.lastname: Samimi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D020128:Inhibitory Concentration 50; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027821:Rutaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D009822:Oils, Volatile; D010936:Plant Extracts