title: The B2 receptor of bradykinin is not essential for the post-exercise increase in glucose uptake by insulin-stimulated mouse skeletal muscle.
abstract: Bradykinin can enhance skeletal muscle glucose uptake (GU), and exercise increases both bradykinin production and muscle insulin sensitivity, but bradykinin's relationship with post-exercise insulin action is uncertain. Our primary aim was to determine if the B2 receptor of bradykinin (B2R) is essential for the post-exercise increase in GU by insulin-stimulated mouse soleus muscles. Wildtype (WT) and B2R knockout (B2RKO) mice were sedentary or performed 60 minutes of treadmill exercise. Isolated soleus muscles were incubated with [³H]-2-deoxyglucose +/-insulin (60 or 100 microU/ml). GU tended to be greater for WT vs. B2RKO soleus with 60 microU/ml insulin (P=0.166) and was significantly greater for muscles with 100 microU/ml insulin (P<0.05). Both genotypes had significant exercise-induced reductions (P<0.05) in glycemia and insulinemia, and the decrements for glucose (approximately 14 %) and insulin (approximately 55 %) were similar between genotypes. GU tended to be greater for exercised vs. sedentary soleus with 60 microU/ml insulin (P=0.063) and was significantly greater for muscles with 100 microU/ml insulin (P<0.05). There were no significant interactions between genotype and exercise for blood glucose, plasma insulin or GU. These results indicate that the B2R is not essential for the exercise-induced decrements in blood glucose or plasma insulin or for the post-exercise increase in GU by insulin-stimulated mouse soleus muscle.
authors.affiliation: Muscle Biology Laboratory, University of Michigan, School of Kinesiology, Ann Arbor, MI, USA.
authors.forename: G G
authors.initials: GG
authors.lastname: Schweitzer
authors.forename: C M
authors.initials: CM
authors.lastname: Castorena
authors.forename: T
authors.initials: T
authors.lastname: Hamada
authors.forename: K
authors.initials: K
authors.lastname: Funai
authors.forename: E B
authors.initials: EB
authors.lastname: Arias
authors.forename: G D
authors.initials: GD
authors.lastname: Cartee
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D005838:Genotype; D006801:Humans; D061386:Insulin, Regular, Human / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D043782:Receptor, Bradykinin B2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D061386:Insulin, Regular, Human; D043782:Receptor, Bradykinin B2