title: Expression of cellular retinoic acid-binding protein I and II (CRABP I and II) in embryonic mouse hearts treated with retinoic acid.
abstract: Cellular retinoic acid binding proteins are considered to be involved in retinoic acid (RA) signaling pathways. Our aim was to compare the expression and localization of cellular retinoic acid binding proteins I and II (CRABP I and II) in embryonic mouse hearts during normal development and after a single teratogenic dose of RA. Techniques such as real-time PCR, RT-PCR, Western blots and immunostaining were employed to examine hearts from embryos at 9-17 dpc. RA treatment at 8.5dpc affects production of CRABP I and II in the heart in the 48-h period. Changes in expression of mRNA for retinaldehyde dehydrogenase II (Raldh2), Crabp1 and Crabp2 genes also occur within the same time window (i.e. 10-11dpc) after RA treatment. In the embryonic control heart these proteins are localized in groups of cells within the outflow tract (OT), and the atrioventricular endocardial cushions. A gradient of labeling is observed with CRABP II but not for CRABP I along the myocardium of the looped heart at 11 dpc; this gradient is abolished in hearts treated with RA, whereas an increase of RALDH2 staining has been observed at 10 dpc in RA-treated hearts. Some populations of endocardial endothelial cells were intensively stained with anti-CRABP II whereas CRABP I was negative in these structures. These results suggest that CRABP I and II are independently regulated during heart development, playing different roles in RA signaling, essential for early remodeling of the heart tube and alignment of the great arteries to their respective ventricles.
authors.affiliation: Department of Pathological Anatomy, Medical University of Warsaw, Warszawa, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Stachurska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Loboda
authors.forename: Justyna
authors.initials: J
authors.lastname: Niderla-Bielińska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Szperl
authors.forename: Michał
authors.initials: M
authors.lastname: Juszyński
authors.forename: Alicja
authors.initials: A
authors.lastname: Jozkowicz
authors.forename: Jozef
authors.initials: J
authors.lastname: Dulak
authors.forename: Anna
authors.initials: A
authors.lastname: Ratajska
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005260:Female; D006321:Heart / Q000187:drug effects* / Q000196:embryology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D011247:Pregnancy; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D018168:Receptors, Retinoic Acid; C082927:retinoic acid binding protein I, cellular; C082928:retinoic acid binding protein II, cellular; D014212:Tretinoin; D050697:Retinal Dehydrogenase