title: Efficient extension of slipped DNA intermediates by DinB is required to escape primer template realignment by DnaQ.
abstract: We show that Escherichia coli DinB polymerase, which creates single-base deletions, prefers to extend slipped DNA substrates with the skipped base at the -4 position. A DinB(Y79L) variant, which extends these substrates less efficiently in vitro, allows the proofreading function of polymerase III to reverse their formation in vivo.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Foti
authors.forename: Graham C
authors.initials: GC
authors.lastname: Walker
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D004258:DNA Polymerase III / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology* / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D017384:Sequence Deletion
chemical_list: D004269:DNA, Bacterial; C094399:DinB protein, E coli; D029968:Escherichia coli Proteins; D050505:Mutant Proteins; D004258:DNA Polymerase III; C468316:dnaQ protein, E coli