title: An extracellular RRR motif flanking the M1 transmembrane domain governs the biogenesis of homomeric neuronal nicotinic acetylcholine receptors.
abstract: We have previously demonstrated that the highly conserved R209, that flanks the M1 transmembrane segment of nicotinic acetylcholine (ACh) receptors, is required for the transport of assembled homomeric neuronal α7 nicotinic ACh receptors to the cell surface. In the present paper we show that basic residues at positions 208 and 210 are necessary for the assembly of α7 receptors. On the contrary, a basic residue at position 210 of α3 subunit decreases the assembly of heteromeric neuronal α3β4 nicotinic ACh receptors. A basic residue at position 210 of the β4 subunit slightly decreases α3β4 receptor expression. We conclude that a pre-M1 RRR motif is necessary for the biogenesis of homomeric α-bungarotoxin-sensitive neuronal α7 nicotinic ACh receptors.
authors.affiliation: Instituto de Neurociencias, Centro Mixto CSIC-Universidad Miguel Hernández, Avenida Ramon y Cajal s/n, 03550 San Juan de Alicante, Spain.
authors.forename: Daiane S
authors.initials: DS
authors.lastname: Alves
authors.forename: Juan
authors.initials: J
authors.lastname: Castello-Banyuls
authors.forename: Clara C
authors.initials: CC
authors.lastname: Faura
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Ballesta
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D002038:Bungarotoxins / Q000378:metabolism; D002417:Cattle; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D007457:Iodine Radioisotopes; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D011725:Pyridines / Q000378:metabolism; D011869:Radioligand Assay; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014316:Tritium; D014982:Xenopus laevis; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D002038:Bungarotoxins; D007457:Iodine Radioisotopes; D011725:Pyridines; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor; C403530:nicotinic receptor alpha3beta4; D014316:Tritium; D001120:Arginine; C082748:epibatidine