title: A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.
abstract: Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.
authors.affiliation: Department of Neurological Surgery, University of Virginia, Charlottesville, Virginia, USA. jsherman0620@gmail.com
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Sherman
authors.forename: Gerard T
authors.initials: GT
authors.lastname: Redpath
authors.forename: Jan A
authors.initials: JA
authors.lastname: Redick
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Purow
authors.forename: Edward R
authors.initials: ER
authors.lastname: Laws
authors.forename: John A
authors.initials: JA
authors.lastname: Jane
authors.forename: Mark E
authors.initials: ME
authors.lastname: Shaffrey
authors.forename: Isa M
authors.initials: IM
authors.lastname: Hussaini
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D016227:Benzoquinones / Q000494:pharmacology; D005404:Fixatives / Q000494:pharmacology; D005909:Glioblastoma / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D010455:Peptides / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D016227:Benzoquinones; D005404:Fixatives; D006023:Glycoproteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D010455:Peptides; C000606024:Prom1 protein, mouse; C004532:quinone