title: Direct injection of native aqueous matrices by achiral-chiral chromatography ion trap mass spectrometry for simultaneous quantification of pantoprazole and lansoprazole enantiomers fractions.
abstract: A two-dimensional liquid chromatography system coupled to ion-trap tandem mass spectrometer (2DLC-IT-MS/MS) was employed for the simultaneous quantification of pantoprazole and lansoprazole enantiomers fractions. A restricted access media of bovine serum albumin octyl column (RAM-BSA C(8)) was used in the first dimension for the exclusion of the humic substances, while a polysaccharide-based chiral column was used in the second dimension for the enantioseparation of both pharmaceuticals. The results described here show good selectivity, extraction efficiency, accuracy, and precision with detection limits of 0.200 and 0.150 μg L(-1) for the enatiomers of pantoprazole and lansoprazole respectively, while employing a small amount (1.0 mL) of native water sample per injection. This work reports an innovative assay for monitoring work, studies of biotic and abiotic enantioselective degradation and temporal changes of enantiomeric fractions.
authors.affiliation: Chemistry Department, Federal University of São Carlos, São Carlos, SP, Brazil.
authors.forename: Juliana Cristina
authors.initials: JC
authors.lastname: Barreiro
authors.forename: Kenia Lourenço
authors.initials: KL
authors.lastname: Vanzolini
authors.forename: Quezia Bezerra
authors.initials: QB
authors.lastname: Cass
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis* / Q000737:chemistry; D006812:Humic Substances / Q000032:analysis; D064747:Lansoprazole; D016018:Least-Squares Analysis; D000077402:Pantoprazole; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D006812:Humic Substances; D014874:Water Pollutants, Chemical; D064747:Lansoprazole; D012710:Serum Albumin, Bovine; D000077402:Pantoprazole