title: Two-photon in vivo imaging of cells.
abstract: In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.
authors.affiliation: The Institute of Optics, University of Rochester, Rochester, NY, USA. dan_christensen@urmc.rochester.edu
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Christensen
authors.forename: Maiken
authors.initials: M
authors.lastname: Nedergaard
mesh_terms: D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton