title: Isoforms of plasma membrane H(+)-ATPase in rice root and shoot are differentially induced by starvation and resupply of NO₃⁻ or NH₄+.
abstract: The goal of this study was to evaluate the effects of nitrogen starvation and resupply in 10 PM H+-ATPase isoforms and the expression of NO₃⁻ and NH₄+ transporters in rice. The net uptake of both forms of NO₃⁻-N or NH₄+-N was increased with its resupply. Resupply of NO₃⁻ resulted in induction of the following PM H+-ATPase isoforms, OsA1, OsA2, OsA5 and OsA7 in the shoots and OsA2, OsA5, OsA7 and OsA8 in the roots. Resupply of NH₄+ resulted in the induction of the following OsA1, OsA3 and OsA7 isoforms in the roots while OsA1 was induced in the shoots. It was observed that increased PM H+-ATPase activity also resulted in increased net uptake of NO₃⁻ and NH₄+. In the roots, OsNRT2.1 and OsNRT2.2 were induced by NO₃⁻ resupply, while OsAMT1.1 and OsAMT1.2 were induced by NH₄+ deficiency. The results showed that the expression of PM H+-ATPase isoforms is related to NO₃⁻ and NH₄+ transporters as well as in which section of the plant it takes place. PM H+-ATPase isoforms OsA2 and OsA7 displayed the strongest induction in response to N resupply, therefore indicating that these genes could be involved in N uptake in rice.
authors.affiliation: Departamento de Solos, Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465 km 7 Seropédica, RJ 23890-000, Brazil. marcusloss@gmail.com
authors.forename: Marcus Vinícius Loss
authors.initials: MV
authors.lastname: Sperandio
authors.forename: Leandro Azevedo
authors.initials: LA
authors.lastname: Santos
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Bucher
authors.forename: Manlio Silvestre
authors.initials: MS
authors.lastname: Fernandes
authors.forename: Sonia Regina
authors.initials: SR
authors.lastname: de Souza
mesh_terms: D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology*; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D000091285:Nitrate Transporters; D009566:Nitrates / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D027321:Anion Transport Proteins; D027682:Cation Transport Proteins; D007527:Isoenzymes; D000091285:Nitrate Transporters; D009566:Nitrates; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; C088952:ammonium transporters, plant; D006180:Proton-Translocating ATPases