title: First report of Klebsiella oxytoca strain coproducing KPC-2 and IMP-8 carbapenemases.
abstract: The study shows for the first time the presence of the Klebsiella oxytoca strain fp10 coproducing plasmid-mediated KPC-2 and IMP-8 carbapenemases. The strain was obtained from the fecal sample of an inpatient and showed high-level resistance to imipenem and ertapenem (MICs > 32 μg/ml). Conjugation experiments demonstrated the transferability of the carbapenem-resistant determinants. The results of plasmid analysis and Southern hybridization revealed that the bla(KPC-2) gene was located on transferable plasmid pFP10-1 (∼54 kb), whereas the bla(IMP-8) gene was on transferable plasmid pFP10-2 (∼180 kb). Analysis of the genetic environment of these two genes has demonstrated that ISKpn6 and ISKpn8 are involved in the spread of the bla(KPC-2) gene, while the transposable elements IS26, intI1, and tniC might contribute to the dissemination of the bla(IMP-8) gene. The chimera of several transposon-associated elements indicated a novel genetic environment of IMP-type metallo-β-lactamase gene in Enterobacteriaceae from China.
authors.affiliation: Department of Clinical Microbiology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 197 Ruijin 2nd Rd., Shanghai 200025, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Jing-Yong
authors.initials: JY
authors.lastname: Sun
authors.forename: Qing-Zhong
authors.initials: QZ
authors.lastname: Liu
authors.forename: Li-Zhong
authors.initials: LZ
authors.lastname: Han
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Huang
authors.forename: Yu-Xing
authors.initials: YX
authors.lastname: Ni
mesh_terms: D003227:Conjugation, Genetic; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C436760:IMP-8 enzyme; C491478:beta-lactamase KPC-2; D001618:beta-Lactamases