title: Detection of DNA sequences using biotinylated probes.
abstract: It was found (1) that biotinylated analogs of dUTP, which contain a biotin molecule covalently bound to the C-5 position of the pyrimidine via an allylamine linker arm, can be used as substrates for DNA polymerase. Figure 1 shows the structure of biotin-11-dUTP, a commonly used substrate for biotinylation by the method of nick translation (2). The substituted DNA molecule will have similar hybridization properties to the un-substituted DNA. After hybridization to the test DNA, the biotin probe can be located by a variety of methods, including incubation with streptavidin-peroxidase conjugate, which will be described here. Fig. 1. Structure of biotin-11-UTP.
authors.affiliation: Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.
authors.forename: J L
authors.initials: JL
authors.lastname: Woodhead
authors.forename: H
authors.initials: H
authors.lastname: Figueiredo
authors.forename: A D
authors.initials: AD
authors.lastname: Malcolm