title: Rhizobium taibaishanense sp. nov., isolated from a root nodule of Kummerowia striata.
abstract: During a study of the diversity and phylogeny of rhizobia in the root nodules of Kummerowia striata grown in north-western China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. The 16S rRNA gene sequences of three of these strains were identical and that of the other strain, which was the only one isolated in Yangling, differed from the others by just 1 bp. The16S rRNA gene sequences of the four strains showed a mean similarity of 99.3 % with the most closely related, recognized species, Rhizobium vitis. The corresponding recA and glnA gene sequences showed similarities with established species of Rhizobium of less than 86.5 % and less than 89.6 %, respectively. These low similarities indicated that the four strains represented a novel species of the genus Rhizobium. The strains were also found to be distinguishable from the closest related, established species (R. vitis) by rep-PCR DNA fingerprinting, analysis of cellular fatty acid profiles and from the results of a series of phenotypic tests. The level of DNA-DNA relatedness between the representative strain CCNWSX 0483(T) and Rhizobium vitis IAM 14140(T) was only 40.13 %. Therefore, a novel species, Rhizobium taibaishanense sp. nov., is proposed, with strain CCNWSX 0483(T) ( = ACCC 14971(T) = HAMBI 3214(T)) as the type strain. In nodulation and pathogenicity tests, none of the four strains of Rhizobium taibaishanense sp. nov. was able to induce any nodule or tumour formation on plants. As no amplicons were detected when DNA from the strains was run in PCR with primers for the detection of nodA, nifH and virC gene sequences, the strains probably do not carry sym or vir genes.
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Li Juan
authors.initials: LJ
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Yao Yao
authors.initials: YY
authors.lastname: Shen
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Jun Peng
authors.initials: JP
authors.lastname: Zhan
authors.affiliation: College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Xinjiang Production and Construction Group, Alar Xinjiang 843300, PR China.|College of Life Sciences, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling Shaanxi 712100, PR China.
authors.forename: Guang Ling
authors.initials: GL
authors.lastname: Cui
authors.forename: Ge Hong
authors.initials: GH
authors.lastname: Wei
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase