title: Optimization of culture conditions in CO2 fixation for succinic acid production using Actinobacillus succinogenes.
abstract: The culture conditions in CO(2) fixation by Actinobacillus succinogenes for succinic acid production were investigated by a model of available CO(2) in a 3-l fermentor. The results from the model analysis showed that the available CO(2) for succinic acid production in the fermentation broth is the sum of HCO(3) (-), CO(3) (2-), and CO(2) influenced by external culture conditions such as medium components, CO(2) partial pressures, and temperature. The optimized conditions for CO(2) supply in a 3-l fermentor were determined as follows: CO(2) partial pressure and stirring speed were maintained at 0.1 MPa and 200 r min(-1), respectively, with a pH of 6.8 and a temperature of 37°C; 0.15 mol l(-1) NaHCO(3) was added. Under the optimized conditions, a CO(2) fixation rate of 0.57 g l(-1) h(-1) was obtained, and a succinic acid concentration of 51.6 g l(-1) with a yield of 75.8% was reached. These results suggest that optimized conditions of CO(2) supply are effective in high succinic acid production and thus have potential applications in succinic acid production and CO(2) fixation.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xinmofan Road 5, 210009, Nanjing, People's Republic of China. yonglanxi@yahoo.com.cn
authors.forename: Yong-lan
authors.initials: YL
authors.lastname: Xi
authors.forename: Ke-quan
authors.initials: KQ
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-jiang
authors.initials: XJ
authors.lastname: Fang
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Zheng
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Sui
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000188:Actinobacillus / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D003470:Culture Media; D002245:Carbon Dioxide; D019802:Succinic Acid