title: Endothelial cell behaviour on gas-plasma-treated PLA surfaces: the roles of surface chemistry and roughness.
abstract: Glow-discharge gas-plasma (GP) treatment has been shown to induce surface modifications such that cell adhesion and growth are enhanced. However, it is not known which gas used in GP treatment is optimal for endothelial cell function. Polylactic acid (PLA) films treated oxygen, argon, or nitrogen GP were characterized using contact angles, scanning electron microscopy, atomic force microscopy, optical profilometry, and x-ray photoelectron spectroscopy. All three GP treatments decreased the carbon atomic concentration and surface roughness and increased the oxygen atomic concentration. Human umbilical vein endothelial cells were cultured on the PLA films for up to 7 days. Based on proliferation and live/dead assays, surface chemistry was shown to have the greatest effect on the attachment, proliferation, and viability of these cells, while roughness did not have a significant influence. Of the different gases, endothelial cell viability, attachment and proliferation were most significantly increased on PLA surfaces treated with oxygen and argon gas plasma.
authors.affiliation: Department of Biomedical Engineering, University of Texas at San Antonio, TX 78249, USA.
authors.forename: Amita
authors.initials: A
authors.lastname: Shah
authors.forename: Sarita
authors.initials: S
authors.lastname: Shah
authors.forename: Gopinath
authors.initials: G
authors.lastname: Mani
authors.forename: Joseph
authors.initials: J
authors.lastname: Wenke
authors.forename: Mauli
authors.initials: M
authors.lastname: Agrawal
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D019344:Lactic Acid / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D055620:Optical Phenomena; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000494:pharmacology*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D058626:Plasma Gases; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)