title: High-density lipoprotein (HDL) from elderly and reconstituted HDL containing glycated apolipoproteins A-I share proatherosclerotic and prosenescent properties with increased cholesterol influx.
abstract: High-density lipoprotein (HDL) is a strong antioxidant, anti-inflammatory, and antisenescence molecule. However, in the current study, HDL from the elderly group (E-HDL) exhibited increased glycation with apolipoprotein (apo) A-I multimerization and decreased phospholipid content. Similarly, glycated apoA-I (gA-I) by fructosylation has a covalently multimerized band without a crosslinker and impaired phospholipid-binding ability. Treatment of human dermal fibroblasts and macrophages with E-HDL and gA-I caused more severe cellular senescence and foam cell formation, respectively; however, treatment with HDL from a young group (Y-HDL) and native apoA-I (nA-I) suppressed senescence and atherosclerosis. E-HDL(3) and reconstituted HDL (rHDL) containing gA-I showed enhanced cholesterol influx into macrophages compared with Y-HDL(3) and nA-I-rHDL. In conclusion, E-HDL and gA-I-rHDL share similar physiologic properties in macrophages and human dermal fibroblasts. E-HDL and gA-I-rHDL exacerbated cellular senescence and atherosclerosis with increased cellular cholesterol influx.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, South Korea.
authors.forename: Ki-Hoon
authors.initials: KH
authors.lastname: Park
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Cho
mesh_terms: D000368:Aged; D000375:Aging / Q000378:metabolism*; D016632:Apolipoprotein A-I / Q000032:analysis*; D050197:Atherosclerosis / Q000503:physiopathology*; D016922:Cellular Senescence / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects*; D005487:Foam Cells / Q000166:cytology; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis* / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D012867:Skin / Q000166:cytology; D055815:Young Adult
chemical_list: D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D002784:Cholesterol