title: Multiphoton microscopy of transdermal quantum dot delivery using two photon polymerization-fabricated polymer microneedles.
abstract: Due to their ability to serve as fluorophores and drug delivery vehicles, quantum dots are a powerful tool for theranostics-based clinical applications. In this study, microneedle devices for transdermal drug delivery were fabricated by means of two-photon polymerization of an acrylate-based polymer. We examined proliferation of cells on this polymer using neonatal human epidermal keratinocytes and human dermal fibroblasts. The microneedle device was used to inject quantum dots into porcine skin; imaging of the quantum dots was performed using multiphoton microscopy.
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina State University, Campus Box 7115, Raleigh, NC 27695-7115, USA.
authors.forename: Shaun D
authors.initials: SD
authors.lastname: Gittard
authors.forename: Philip R
authors.initials: PR
authors.lastname: Miller
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Boehm
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Ovsianikov
authors.forename: Boris N
authors.initials: BN
authors.lastname: Chichkov
authors.forename: Jeremy
authors.initials: J
authors.lastname: Heiser
authors.forename: John
authors.initials: J
authors.lastname: Gordon
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Monteiro-Riviere
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Narayan
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D016503:Drug Delivery Systems / Q000295:instrumentation*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000737:chemistry; D008845:Microinjections / Q000379:methods; D008856:Microscopy, Fluorescence; D009339:Needles; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots; D012867:Skin / Q000737:chemistry* / Q000166:cytology; D013552:Swine
chemical_list: D011108:Polymers