title: [Preparation of monoclonal antibodies specific for the major tegument protein pp65 of human herpesvirus type 5].
abstract: A panel containing 11 types of monoclonal antibodies (mAb) specific for the recombinant protein pp65 of human herpesvirus type 5 was constructed. Enzyme immunoassay and immunoblotting of the immunochemical properties of mAb indicated that mAb could interact well with both recombinant and native pp65 antigen. Testing the suitability of mAb for an immunocytochemical study established that mAb 5F10 was highly effective in detecting the major tegument protein pp65 in the persistently infected Vero cells. A combination of the immunochemical properties of mAb enables them to be recommended for the immunodiagnosis of human cytomegalovirus infection.
authors.forename: E I
authors.initials: EI
authors.lastname: Kazachinskaia
authors.forename: M A
authors.initials: MA
authors.lastname: Susloparov
authors.forename: V B
authors.initials: VB
authors.lastname: Loktev
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry / Q000276:immunology*; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology*; D002522:Chlorocebus aethiops; D003587:Cytomegalovirus / Q000276:immunology* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D010750:Phosphoproteins / Q000276:immunology* / Q000378:metabolism; D014709:Vero Cells; D014763:Viral Matrix Proteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000914:Antibodies, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa