title: ISSR markers as a tool for the assessment of genetic diversity in Passiflora.
abstract: Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei's genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon-Weaver diversity index was 0.32, indicating good resolution of genetic diversity in passion fruit germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies.
authors.affiliation: Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil.
authors.forename: Lucas Ferraz
authors.initials: LF
authors.lastname: dos Santos
authors.forename: Eder Jorge
authors.initials: EJ
authors.lastname: de Oliveira
authors.forename: Aline
authors.initials: A
authors.lastname: dos Santos Silva
authors.forename: Fabiana Moraes
authors.initials: FM
authors.lastname: de Carvalho
authors.forename: Juliana Leles
authors.initials: JL
authors.lastname: Costa
authors.forename: Juliano Gomes
authors.initials: JG
authors.lastname: Pádua
mesh_terms: D001938:Brazil; D005819:Genetic Markers; D014644:Genetic Variation; D029598:Passiflora / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D005819:Genetic Markers