title: Elasticity of cardiac cells on the polymer substrates with different stiffness: an atomic force microscopy study.
abstract: Influences of substrate stiffness on mechanical properties of cardiac myocytes and fibroblasts were investigated by cell elasticity measurement with atomic force microscopy. The cells were cultured on collagen-coated polyacrylamide substrates with gradient rigidity. While cardiac myocytes showed no evident change in cell elasticity on different substrates, cardiac fibroblasts displayed the non-monotonic dependence on substrate stiffness with a maximum elastic modulus. Moreover, the elasticity change of cardiac fibroblasts with substrates stiffness was found to be regulated by actin filaments. Study of the effect of substrate stiffness on cell elasticity for different cardiac cells provides new information for the better understanding of cardiac physiology and pathology.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shi
authors.forename: Lei
authors.initials: L
authors.lastname: Qin
authors.forename: Xuejie
authors.initials: X
authors.lastname: Zhang
authors.forename: Kangmin
authors.initials: K
authors.lastname: He
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xiong
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Fang
authors.forename: Youyi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D008841:Actin Cytoskeleton / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology; D018625:Microscopy, Atomic Force; D032383:Myocytes, Cardiac / Q000166:cytology*; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D003094:Collagen; D011108:Polymers; D000099166:Polyacrylamides