title: Exogenous fibroblast growth factor 8 rescues development of mouse diastemal vestigial tooth ex vivo.
abstract: Regression of vestigial tooth buds results in the formation of the toothless diastema, a unique feature of the mouse dentition. Revitalization of the diastemal vestigial tooth bud provides an excellent model for studying tooth regeneration and replacement. It has been previously shown that suppression of fibroblast growth factor (FGF) signaling in the diastema results in vestigial tooth bud regression. In this study, we report that application of exogenous FGF8 to the mouse embryonic diastemal region rescues diastemal tooth development. However, this rescue of diastemal tooth development occurs only in an isolated diastemal regions and not in the mandibular quadrant, which includes the incisor and molar germs. FGF8 promotes cell proliferation and inhibits apoptosis in diastemal tooth epithelium, and revitalizes the tooth developmental program, as evidenced by the expression of genes critical for normal tooth development. Our results also support the idea that the adjacent tooth germs contribute to the suppression of diastemal vestigial tooth buds by means of multiple signals.
authors.affiliation: Hubei-MOST KLOS and KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Guohua
authors.initials: G
authors.lastname: Yuan
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Yanding
authors.initials: Y
authors.lastname: Zhang
authors.forename: YiPing
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003970:Diastema / Q000196:embryology*; D005260:Female; D051524:Fibroblast Growth Factor 8 / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008297:Male; D051379:Mice; D009805:Odontogenesis / Q000187:drug effects / Q000235:genetics; D011247:Pregnancy; D014070:Tooth / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D014083:Tooth Germ / Q000187:drug effects / Q000196:embryology
chemical_list: D051524:Fibroblast Growth Factor 8