title: [The absorption-elution technique with the use of highly active proteases: a novel technology for the detection of Rhesus D antigen in bloodstains].
abstract: The proposed method for the specific detection of Rhesus D antigen in 6 month old bloodstains is based on the use of modified immunoglobulins (anti-rhesus D peptides) in the absorption-elution reaction (AER). These peptides were prepared by an original method using polyclonal anti-rhesus D antibodies and standard erythrocytes preliminarily treated with a highly active protease. Specific identification of D antigen of the Rhesus system obviates the problem of poor AER specificity and thereby significantly improves the quality of forensic biological expertise.
authors.forename: I V
authors.initials: IV
authors.lastname: Kondratova
authors.forename: A A
authors.initials: AA
authors.lastname: Gusarov
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D001801:Blood Stains; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D010447:Peptide Hydrolases / Q000737:chemistry*; D012204:Rh-Hr Blood-Group System / Q000032:analysis* / Q000737:chemistry
chemical_list: D000906:Antibodies; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen; D010447:Peptide Hydrolases