title: Dietary toxicity of single-walled carbon nanotubes and fullerenes (C60) in rainbow trout (Oncorhynchus mykiss).
abstract: The objective of this investigation was to compare the toxicity of two manufactured carbon nanomaterials (CNs) to determine if shape influenced toxicity. Juvenile rainbow trout Oncorhynchus mykiss were fed a control diet (no CN addition), or a diet supplemented with 500 mg single-walled carbon nanotubes (SWCNT) kg(-1) or 500 mg C(60) kg(-1) for six weeks. Fish growth, haematology, tissue ion concentrations, histopathology, osmoregulation, and biochemistry were evaluated. At week 4, but not on weeks 2 and 6, significant elevation in brain TBARS (an indication of lipid peroxidation) was observed in fish exposed to SWCNTs (16.2 ± 1.38 nmol mg(-1) protein) compared to the control (9.11 ± 0.81 nmol mg(-1) protein) and fish exposed to C(60) (8.28 ± 0.56 nmol mg(-1) protein). No other significant treatment-related differences were observed. Results indicate that dietary exposure to SWCNTs and C(60) in rainbow trout did not result in overt toxicity.
authors.affiliation: School of Biomedical and Biological Sciences, University of Plymouth, Devon, UK.
authors.forename: Thomas W K
authors.initials: TW
authors.lastname: Fraser
authors.forename: Helena C
authors.initials: HC
authors.lastname: Reinardy
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Shaw
authors.forename: Theodore B
authors.initials: TB
authors.lastname: Henry
authors.forename: Richard D
authors.initials: RD
authors.lastname: Handy
mesh_terms: D000818:Animals; D004032:Diet; D005260:Female; D037741:Fullerenes / Q000378:metabolism / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D037742:Nanotubes, Carbon / Q000633:toxicity; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D014882:Water-Electrolyte Balance / Q000187:drug effects
chemical_list: D037741:Fullerenes; D037742:Nanotubes, Carbon; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D005978:Glutathione