title: [Effect of longterm lower Porphyromonas gingivalis infection on the progression of atherosclerosis in apolipoprotein E-knocked out mice].
abstract: OBJECTIVE
  To assess the effect of longterm and lower oral inoculation with Porphyromonas gingivalis (Pg) on the progression of atherosclerosis in apolipoprotein E-knocked out (ApoE(-/-)) mice.
  
  
  METHODS
  Six-week-old male ApoE(-/-) mice were inoculated orally with 0.1 ml live Pg(10(13)/L) or bouillon culture-medium quintic per week for 15 consecutive weeks, altogether 75 times of inoculations. The lesion area of atherosclerosis in the aortic tree was measured by en face quantification by red oil O staining method. The atherosclerotic lesion was examined by histopathology. The levels of total cholesterol and triglycerides were compared.
  
  
  RESULTS
  At 22 weeks after inoculation, the mean atherosclerotic lesion area in inoculated mice was (98 363.68 ± 12 043.00) µm(2), which was significantly greater than that in noninoculated mice, which was (62 985.06 ± 7419.64) µm(2) (P = 0.035).
  
  
  CONCLUSIONS
  Longterm lower oral inoculation of Pg can accelerate the progression of atherosclerosis in apolipoprotein E-knocked out mice.
authors.affiliation: Department of Periodontology, School of Stomatology, Nanjing University & Nanjing Stomatological Hospital, Nanjing 210008, China.
authors.forename: Xu-na
authors.initials: XN
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Wu
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Sun
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology*; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000097:blood / Q000150:complications* / Q000473:pathology; D016866:Bacteroidaceae Infections / Q000150:complications* / Q000382:microbiology; D002784:Cholesterol / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016966:Porphyromonas gingivalis; D011897:Random Allocation; D014280:Triglycerides / Q000097:blood
chemical_list: D001057:Apolipoproteins E; D014280:Triglycerides; D002784:Cholesterol