title: The herbal preparation Padma® 28 protects against neurotoxicity in PC12 cells.
abstract: Padma® 28 is a multicompound herbal preparation based on the camphor formulas from traditional Tibetan medicine (TTM). It contains a variety of different secondary plant substances, which include terpenes and polyphenols such as flavonoids and tannins. As a rich source of antioxidant polyphenols, this herbal Padma 28 preparation seems to be a promising candidate for the treatment of degenerative diseases such as Alzheimer's disease (AD), a condition involving oxidative stress. Moreover, polyphenols have also been shown to mitigate AD neuropathology. The study investigated the protective effect of Padma 28 and of certain polyphenols on the neurotoxicity of PC12 cells induced by the neurotoxins: amyloid-beta (Aβ), glutamate, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 3-nitropropionate (3-NP), known to be involved in AD, Parkinson's disease (PD), amyotrophic-lateral-sclerosis (ALS) and Huntington's disease (HD), respectively. The decrease in cell viability induced by each of the toxins was significantly attenuated by Padma 28 treatment. Also, a decrease in the oxidative capacity of PC12 cells treated with Padma 28 was noted, indicating that the decrease in cell viability induced by the toxins might have been the result of an oxidative stress which could be attenuated by Padma 28 acting as a potent antioxidant. Padma 28, which is available in Europe and USA, seems to be a promising candidate for the treatment of CNS diseases.
authors.affiliation: Dental Research, Faculty of Dental Medicine, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
authors.forename: Isaac
authors.initials: I
authors.lastname: Ginsburg
authors.forename: Lea
authors.initials: L
authors.lastname: Rozenstein-Tsalkovich
authors.forename: Erez
authors.initials: E
authors.lastname: Koren
authors.forename: Hanna
authors.initials: H
authors.lastname: Rosenmann
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000517:prevention & control; D016229:Amyloid beta-Peptides / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005060:Europe; D005971:Glutamates / Q000633:toxicity; D006801:Humans; D020267:MPTP Poisoning / Q000139:chemically induced / Q000517:prevention & control; D008518:Medicine, East Asian Traditional; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D009498:Neurotoxins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D028321:Plant Preparations / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols / Q000494:pharmacology; D051381:Rats; D018609:Tibet; D014481:United States
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; D005971:Glutamates; D009498:Neurotoxins; D010936:Plant Extracts; D028321:Plant Preparations; D059808:Polyphenols; C066069:Padma 28