title: Correlation of the expression of toll-like receptors in monocyte-derived dendritic cells with prognosis of chronic severe hepatitis B.
abstract: OBJECTIVE
  This study aims to measure the expression of toll-like receptors (TLR) in monocyte-derived dendritic cells (MoDC) from chronic severe hepatitis B (CSHB), to assess the contribution of TLRs in CSHB.
  
  
  METHODS
  Peripheral blood was collected from 40 CSHB patients, 30 chronic hepatitis B (CHB) patients, and 30 healthy individuals who served as healthy controls (HCs). Purified monocytes were isolated by a combination of Histopaque-1.077 and CD14 Microbeads. MoDCs were induced with granulocyte macrophage colony-stimulating factor and interleukin-4 for 6 days from CD14(+) monocytes. The expression of TLRs in MoDC was measured using real-time PCR and flow cytometry.
  
  
  RESULTS
  The expressions of TLR-1, -2, -7 were significantly higher in MoDC of CSHB than that of HCs, of which the level of TLR-3 was decreased. Particularly in CSHB patients, the TLR-3 expression was further decreased compared to CHB patients. In non-survival CSHB patients, TLR-3 level was significantly decreased, while TLR-2 expression was dramatically increased. Linear correlation analysis demonstrated significant correlations between TLR-3 level and disease severity markers (total bilirubin, prothrombin activity, creatinine, white blood cell count, and maximum volume of ascitic fluid) in individual CSHB patients.
  
  
  CONCLUSIONS
  TLR-2 and TLR-3 may be involved in the pathogenesis of CSHB, and TLR-3 may influence the prognosis of CSHB.
authors.affiliation: Department of Infectious Diseases, Huashan Hospital, Shanghai, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Ming Quan
authors.initials: MQ
authors.lastname: Chen
authors.forename: Zhi Ping
authors.initials: ZP
authors.lastname: Qian
authors.forename: Meng Qi
authors.initials: MQ
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Jian Ming
authors.initials: JM
authors.lastname: Zheng
authors.forename: Xin Yu
authors.initials: XY
authors.lastname: Wang
authors.forename: Guang Feng
authors.initials: GF
authors.lastname: Shi
mesh_terms: D016022:Case-Control Studies; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D019694:Hepatitis B, Chronic / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D051193:Toll-Like Receptors