title: Micropatterns of cell adhesive proteins with poly(ethylene oxide)-block-Poly(4-vinylpyridine) diblock copolymer.
abstract: Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(ethylene oxide-block-poly(4-vinylpyridine) (PEO-b-P4VP) as a non-adhesive background to construct micropatterns of cell adhesive proteins. In the method presented, PEO-b-P4VP is used for its antifouling properties and at the same time, as a photosensitive material to define the micropatterns. The irradiation of PEO-b-P4VP with a short wavelength UV light through photolithographic mask, causes the polymer to crosslink and immobilize in the areas exposed. In the areas non-exposed the polymer can be removed. These areas can be subsequent back filled with the adhesive protein of interest to produce the final micropatterned cell chips.
authors.affiliation: Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3, Research Link, Singapore 117602, Singapore.
authors.forename: J
authors.initials: J
authors.lastname: Racine
authors.forename: H
authors.initials: H
authors.lastname: Cheradame
authors.forename: Y S
authors.initials: YS
authors.lastname: Chu
authors.forename: J P
authors.initials: JP
authors.lastname: Thiery
authors.forename: I
authors.initials: I
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D005353:Fibronectins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D005353:Fibronectins; D014753:Vinyl Compounds; C520176:poly(4-vinylpyridine)-block-poly(ethylene oxide); D011092:Polyethylene Glycols