title: DNA detection using enzymatic signal production and SERS.
abstract: A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.
authors.affiliation: Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral St., Glasgow, G1 1XL.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Dougan
authors.forename: Douglas
authors.initials: D
authors.lastname: MacRae
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
mesh_terms: D004247:DNA / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; C430823:endodeoxyribonuclease BstNBI; D015252:Deoxyribonucleases, Type II Site-Specific