title: The acquisition of antigen cross-presentation function by newly formed dendritic cells.
abstract: The development of Ag-presenting functions by murine dendritic cells (DCs) of the CD8(+) DC lineage was studied using a Flt-3 ligand stimulated bone-marrow culture system. Although newly formed DCs of this lineage are capable of Ag uptake and efficient presentation to T cells on MHC class II, they initially lack the ability to cross-present exogenous Ags on MHC class I. Cross-presentation capacity is acquired as a subsequent maturation step, promoted by cytokines such as GM-CSF. The development of cross-presentation capacity by the DCs in these cultures may be monitored by the parallel development of DC surface expression of CD103. However, the expression of CD103 and cross-presentation capacity are not always linked; therefore, CD103 is not an essential part of the cross-presentation machinery. These results explain the considerable variability in CD103 expression by CD8(+) DCs as well as the findings that not all DCs of this lineage are capable of cross-presentation.
authors.affiliation: Walter and Eliza Hall Institute, Parkville, Victoria 3052, Australia.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sathe
authors.forename: Joanne
authors.initials: J
authors.lastname: Pooley
authors.forename: David
authors.initials: D
authors.lastname: Vremec
authors.forename: Justine
authors.initials: J
authors.lastname: Mintern
authors.forename: Jun-O
authors.initials: JO
authors.lastname: Jin
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villadangos
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D016827:CD8 Antigens / Q000096:biosynthesis / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D045142:Cross-Priming / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D039001:Integrin alpha Chains / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D015703:Antigens, CD; D016827:CD8 Antigens; D039001:Integrin alpha Chains; C091367:alpha E integrins