title: A study of the inhibition/promotion effects of sodium-copper chlorophyllin (SCC)-mediated mutagenesis in somatic cells of Drosophila.
abstract: Sodium-copper chlorophyllin (SCC), a copper-porphyrin complex, has been shown to act as an inhibitor as well as a promoter of DNA-damage induction by a variety of mutagens in several test systems. In order to investigate the basis of this dual effect, experiments were carried out to compare the influence of pretreatment with intact SCC and that of its constituents, the metal-free protoporphyrin (PP-IX) and copper as CuCl(2). The wing-spot test was employed to monitor mutational events in somatic cells of Drosophila melanogaster. Heterozygous mwh+/+flr(3) larvae were treated for 24h with SCC, PP-IX, CuCl(2) or sucrose. Following this treatment, one group of larvae were immediately allowed to feed on instant medium containing 0.5mM N-nitroso-N-ethylurea (ENU) dissolved in phosphate buffer to reach pH 6. The remaining larvae received treatment with ENU with a delay of 1, 2 or 3days (DTD). Results revealed an (a) overall inhibitory effect for 0-DTD and 1-DTD after pretreatment with SCC, (b) only in 0-DTD after PP-IX, and (c) in all DTDs after treatment with CuCl(2). These results provide evidence that the copper ion plays a central role in the antimutagenic effect of SCC, and for a sustained period of time. Pretreatment with SCC and PP-IX produced a promoter effect at 2-DTD and 3-DTD. The results could be explained as an effect of the accumulation of metal-free porphyrin following the dissociation of the copper-porphyrin complex (SCC), the copper-ion reaching proteins to form complexes and participated in anabolic pathways.
authors.affiliation: Departamento de Biología, Instituto Nacional de Investigaciones Nucleares (ININ), Carretera México-Toluca S/N, La Marquesa, Ocoyoacac, México CP. 52750, Mexico. emilio.pimentel@inin.gob.mx
authors.forename: E
authors.initials: E
authors.lastname: Pimentel
authors.forename: M P
authors.initials: MP
authors.lastname: Cruces
authors.forename: S
authors.initials: S
authors.lastname: Zimmering
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002735:Chlorophyllides / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D003300:Copper / Q000494:pharmacology*; D004330:Drosophila / Q000235:genetics; D005038:Ethylnitrosourea / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D016587:Antimutagenic Agents; D002735:Chlorophyllides; D009153:Mutagens; D011166:Porphyrins; D003300:Copper; C007020:chlorophyllin; D005038:Ethylnitrosourea