title: Direct conversion of inulin and extract of tubers of Jerusalem artichoke into single cell oil by co-cultures of Rhodotorula mucilaginosa TJY15a and immobilized inulinase-producing yeast cells.
abstract: In this study, it was found that the immobilized inulinase-producing cells of Pichia guilliermondii M-30 could produce 169.3 U/ml of inulinase activity while the free cells of the same yeast strain only produced 124.3 U/ml of inulinase activity within 48 h. When the immobilized inulinase-producing yeast cells were co-cultivated with the free cells of Rhodotorula mucilaginosa TJY15a, R. mucilaginosa TJY15a could accumulate 53.2% oil from inulin in its cells and cell dry weight reached 12.2g/l. Under the similar conditions, R. mucilaginosa TJY15a could accumulate 55.4% (w/w) oil from the extract of Jerusalem artichoke tubers in its cells and cell dry weight reached 12.8 g/l within 48 h. When the co-cultures were grown in 2l fermentor, R. mucilaginosa TJY15a could accumulate 56.6% (w/w) oil from the extract of Jerusalem artichoke tubers in its cells and cell dry weight reached 19.6g/l within 48 h. Over 90.0% of the fatty acids from the yeast strain TJY15a grown in the extract of Jerusalem artichoke tubers was C(16:0), C(18:1) and C(18:2), especially C(18:1) (50.6%).
authors.affiliation: Unesco Chinese Center of Marine Biotechnology and Institute of Marine Biodiversity and Evolution, Ocean University of China, No. 5 Yushan Road, Qingdao, China.
authors.forename: Chun-Hai
authors.initials: CH
authors.lastname: Zhao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chi
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: Guo
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Song
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
mesh_terms: D018920:Coculture Techniques; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006368:Helianthus / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D012248:Rhodotorula / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase