title: Tryptase and chymase are angiogenic in vivo in the chorioallantoic membrane assay.
abstract: Human mast cells (MCs) are divided in two types depending on the expression of tryptase and chymase in their granules. Literature data indicate that both tryptase and chymase are angiogenic, but there is currently no evidence of their direct angiogenic activity in vivo. In this study, we have investigated the capacity of tryptase and chymase to promote vasoproliferation in chick embryo chorioallantoic membrane (CAM), a well established in vivo assay to study angiogenesis and anti-angiogenesis. The results showed that both tryptase and chymase stimulate angiogenesis and that the response is similar to that obtained with vascular endothelial growth factor (VEGF), a well-known angiogenic cytokine, and confirm the angiogenic activity of these two proteases stored in MC granules.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy. ribatti@anatomia.uniba.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.forename: Girolamo
authors.initials: G
authors.lastname: Ranieri
authors.forename: Beatrice
authors.initials: B
authors.lastname: Nico
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Benagiano
authors.forename: Enrico
authors.initials: E
authors.lastname: Crivellato
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D053818:Chymases / Q000235:genetics / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000201:enzymology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D053802:Tryptases / Q000235:genetics / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D053818:Chymases; D053802:Tryptases