title: Coarse-grained dynamics of protein synthesis in a cell-free system.
abstract: A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.
authors.affiliation: Department of Materials and Interfaces, the Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Eyal
authors.initials: E
authors.lastname: Karzbrun
authors.forename: Jonghyeon
authors.initials: J
authors.lastname: Shin
authors.forename: Roy H
authors.initials: RH
authors.lastname: Bar-Ziv
authors.forename: Vincent
authors.initials: V
authors.lastname: Noireaux
mesh_terms: D002474:Cell-Free System / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D029968:Escherichia coli Proteins; D012333:RNA, Messenger