title: β1 integrin regulates MMP-10 dependant tubulogenesis in human lymphatic endothelial cells.
abstract: Lymphatic vessel growth requires extensive remodeling of the extracellular matrix, a process hypothesized to be related to the expression and function of the matrix metalloproteinases. We used a protein based screening strategy to demonstrate increased matrix matalloproteinase-10 expression in human lymphatic endothelial cells undergoing collagen I induced tubulogenesis. Knock-down experiments showed that matrix metalloproteinase-10 regulated lymphatic endothelial cell tubulogenesis. β1 integrin signaling via the ERK/MAPK pathway increased matrix metalloproteinase-10 mRNA and protein expression in human lymphatic endothelial cells. These findings demonstrate a novel mechanism by which β1 integrin regulates matrix metalloproteinase-10 expression during lymphatic vessel remodeling.
authors.affiliation: Boys Town National Research Hospital, Omaha, NE 68124, United States.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Steele
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Schieler
authors.forename: Philip M
authors.initials: PM
authors.lastname: Kelley
authors.forename: Richard M
authors.initials: RM
authors.lastname: Tempero
mesh_terms: D002478:Cells, Cultured; D024042:Collagen Type I / Q000494:pharmacology; D017077:Culture Media, Conditioned; D004729:Endothelium, Lymphatic / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D053506:Matrix Metalloproteinase 10 / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D040081:Protein Array Analysis; D015398:Signal Transduction
chemical_list: D024042:Collagen Type I; D017077:Culture Media, Conditioned; D019012:Integrin beta1; D020928:Mitogen-Activated Protein Kinases; C511770:MMP10 protein, human; D053506:Matrix Metalloproteinase 10