title: [Effects of β-amyloid-induced microglial inflammatory supernatant on neuronal apoptosis].
abstract: OBJECTIVE
  To study the effects of neuronal apoptosis under the induction of β-amyloid inflammatory supernatant.
  
  
  METHODS
  The neurons were intervened by β-amyloid-induced inflammatory supernatant at the concentration of Aβ1-42 at 125 nmol/L. And the expressions of Bcl-2, caspase-3, PARP and neuronal apoptosis were detected.
  
  
  RESULTS
  The caspase-3 (14.2 ± 1.8), Bcl-2 (10.6 ± 0.8) positive cells of microglia inflammatory supernatant stimulated group were significantly elevated than the control (2.2 ± 0.6, 5.0 ± 0.3, P < 0.01). Nuclear chromatin was uniform yellow-green fluorescent. And there was significant difference of neuronal apoptosis between microglia inflammatory supernatant group and Aβ1-42 directly stimulated group.
  
  
  CONCLUSIONS
  Neuronal apoptosis is induced by caspase-3 in β-amyloid inflammatory supernatant. One of the important causes is chronic inflammatory process of activated microglia by Aβ.
authors.affiliation: Department of Neurology, Henan Provincial People's Hospital, Zhengzhou 450003, China. liwei71@126.com
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ai-qin
authors.initials: AQ
authors.lastname: Suo
authors.forename: Jie-wen
authors.initials: JW
authors.lastname: Zhang
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Zhao
authors.forename: Ji-hui
authors.initials: JH
authors.lastname: Ge
authors.forename: De-ying
authors.initials: DY
authors.lastname: Qin
authors.forename: Xian-jun
authors.initials: XJ
authors.lastname: He
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Jiang
mesh_terms: D016229:Amyloid beta-Peptides / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D007249:Inflammation; D017628:Microglia / Q000378:metabolism* / Q000473:pathology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016229:Amyloid beta-Peptides