title: Supplementation of cryomedium with ascorbic acid-2-glucoside (AA2G) improves human sperm post-thaw motility.
abstract: OBJECTIVE
  To evaluate the efficacy of ascorbic acid-2-glucoside (AA2G) supplementation in cryomedium to improve human sperm motility preservation through the freeze-thaw process.
  
  
  METHODS
  Prospective research study.
  
  
  METHODS
  University-based andrology laboratory.
  
  
  METHODS
  One hundred thirty-four patients attending the andrology laboratory for a regular semen analysis between April 2009 and May 2010.
  
  
  METHODS
  Supplementation of cryomedium with ascorbate or AA2G.
  
  
  METHODS
  Endogenous semen ascorbic acid concentrations, semen lipid peroxidation levels, progressive sperm motility.
  
  
  RESULTS
  AA2G-supplemented samples showed improved sperm motility preservation after freezing compared with control samples. No significant difference was found in motility preservation between control samples and those supplemented with ascorbate.
  
  
  CONCLUSIONS
  Our data indicate that AA2G confers a protective effect on human sperm motility preservation through the freeze-thaw cycle. This same effect was not seen with ascorbate. Further investigation is required to elucidate a concrete mechanism of action.
authors.affiliation: Andrology and IVF Laboratories, Department of Surgery, University of Utah School of Medicine, Salt Lake City, Utah 84108, USA.
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Jenkins
authors.forename: Kenneth I
authors.initials: KI
authors.lastname: Aston
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Carrell
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005615:Freezing; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; C065378:ascorbic acid 2-O-glucoside; D001205:Ascorbic Acid