title: Genetic control of amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR.
abstract: Bacillus subtilis is capable of degrading fructosamines. The phosphorylation and the cleavage of the resulting fructosamine 6-phosphates is catalyzed by the frlD and frlB gene products, respectively. This study addresses the physiological importance of the frlBONMD genes (formerly yurPONML), revealing the necessity of their expression for growth on fructosamines and focusing on the complex regulation of the corresponding transcription unit. In addition to the known regulation by the global transcriptional regulator CodY, the frl genes are repressed by the convergently transcribed FrlR (formerly YurK). The latter causes repression during growth on substrates other than fructosamines. Additionally, we identified in the first intergenic region of the operon an FrlR binding site which is centrally located within a 38-bp perfect palindromic sequence. There is genetic evidence that this sequence, in combination with FrlR, contributes to the remarkable decrease in the transcription downstream of the first gene of the frl operon.
authors.affiliation: Henkel AG & Co. KGaA, Biotechnology, Henkelstr. 67, 40589 Düsseldorf, Germany.
authors.forename: Veronika Maria
authors.initials: VM
authors.lastname: Deppe
authors.forename: Stephanie
authors.initials: S
authors.lastname: Klatte
authors.forename: Johannes
authors.initials: J
authors.lastname: Bongaerts
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Maurer
authors.forename: Timothy
authors.initials: T
authors.lastname: O'Connell
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Meinhardt
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism; D019270:Fructosamine / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D009876:Operon; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; D019270:Fructosamine