title: In vivo evaluation of hypoglycemic activity of Aloe spp. and identification of its mode of action on GLUT-4 gene expression in vitro.
abstract: The present study evaluated the hypoglycemic activity of Aloe extract on streptozotocin-induced diabetic mice and focuses its effect on GLUT-4 gene expression under in vitro cell-culture system. Administration of extract at the dosage of 130 mg/kg body weight per day for 4 weeks resulted in significant decrease in blood glucose and total cholesterol in streptozotocin (60 mg/kg body weight) induced diabetic mice. The hypoglycemic effect was compared with metformin. The activities of carbohydrate metabolizing enzymes were brought back to near normal level after the treatment and glucose homeostasis was maintained. Lyophilized aqueous Aloe extract (1 mg/ml) upregulated the GLUT-4 mRNA synthesis in mouse embryonic NIH/3T3 cells.
authors.affiliation: Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, US Nagar, India.
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.forename: Bhavna
authors.initials: B
authors.lastname: Sharma
authors.forename: Neha R
authors.initials: NR
authors.lastname: Tomar
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
authors.forename: Atul K
authors.initials: AK
authors.lastname: Gupta
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D005951:Glucose Tolerance Test; D051275:Glucose Transporter Type 4 / Q000235:genetics*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023382:Toxicity Tests, Acute; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D008055:Lipids; D010936:Plant Extracts; D012333:RNA, Messenger; C495693:Slc2a4 protein, mouse; D006003:Glycogen; D002784:Cholesterol; D006593:Hexokinase; D005952:Glucose-6-Phosphatase