title: Rapid and simple polymerase chain reaction-based diagnostic assays for GM2 gangliosidosis variant 0 (Sandhoff-like disease) in Japanese domestic cats.
abstract: Polymerase chain reaction (PCR)-based assays combined with microchip electrophoresis were developed and evaluated for diagnosis and genotyping of GM2 gangliosidosis variant 0 (Sandhoff-like disease) in Japanese domestic cats. A preliminary genotyping survey was carried out in the population of Japanese domestic cats (1,015 cats in total) in southern Japan. Three kinds of assays including PCR primer-induced restriction analysis (PIRA) and mutagenically separated (MS)-PCR were carried out using blood-stained Flinders Technology Associates filter papers (FTA cards) as templates. The PCR products were analyzed by both agarose gel and microchip electrophoreses. All assays were sufficient to determine the genotypes of this disease, but MS-PCR offered the most rapid and simplest test, as it does not need the restriction enzyme step required in PCR-PIRA. The use of microchip electrophoresis in combination with FTA cards for sampling could shorten the time required for genotyping and simplify the procedure as well. The genotyping survey in the current study did not find any cats that possessed the mutant allele, suggesting that the prevalence of this allele is low (<0.1%) in southern Japan.
authors.affiliation: Laboratory of Clinical Pathology, Department of Veterinary Clinical Sciences, Faculty of Agriculture, Kagoshima University, 1-21-24 Kohrimoto, Kagoshima 890-0065, Japan.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Rahman
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Shoubudani
authors.forename: Keijiro
authors.initials: K
authors.lastname: Mizukami
authors.forename: Hye-Sook
authors.initials: HS
authors.lastname: Chang
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Hossain
authors.forename: Akira
authors.initials: A
authors.lastname: Yabuki
authors.forename: Sawane
authors.initials: S
authors.lastname: Mitani
authors.forename: Takashi
authors.initials: T
authors.lastname: Higo
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamato
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000201:enzymology / Q000235:genetics; D002415:Cats; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005838:Genotype; D054819:Hexosaminidase B / Q000235:genetics* / Q000378:metabolism; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide; D012497:Sandhoff Disease / Q000175:diagnosis / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*
chemical_list: D004247:DNA; D054819:Hexosaminidase B