title: Simultaneous determination of iron and copper in children's sera by FAAS.
abstract: A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L-1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L-1 HCl standard. During method's optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.
authors.affiliation: Faculty of Pharmacy and Biochemistry Zagreb, University of Zagreb, Croatia. sluter@pharma.hr
authors.forename: Svjetlana
authors.initials: S
authors.lastname: Luterotti
authors.forename: Tončica Vukman
authors.initials: TV
authors.lastname: Kordić
authors.forename: Slavica
authors.initials: S
authors.lastname: Dodig
mesh_terms: D002648:Child; D003300:Copper / Q000097:blood*; D006801:Humans; D007501:Iron / Q000097:blood*; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013997:Time Factors
chemical_list: D003300:Copper; D007501:Iron