title: Oxidative modification of ferritin induced by hydrogen peroxide.
abstract: Excess free iron generates oxidative stress that may contribute to the pathogenesis of various causes of neurodegenerative diseases. In this study, we assessed the modification of ferritin induced by H(2)O(2). When ferritin was incubated with H(2)O(2), the degradation of ferritin L-chain increased with the H(2)O(2) concentration whereas ferritin H-chain was remained. Free radical scavengers, azide, thiourea, and N-acetyl-(L)-cysteine suppressed the H(2)O(2)-mediated ferritin modification. The iron specific chelator, deferoxamine, effectively prevented H(2)O(2)-mediated ferritin degradation in modified ferritin. The release of iron ions from ferritin was increased in H(2)O(2) concentration-dependent manner. The present results suggest that free radicals may play a role in the modification and iron releasing of ferritin by H(2)O(2). It is assumed that oxidative damage of ferritin by H(2)O(2) may induce the increase of iron content in cells and subsequently lead to the deleterious condition.
authors.affiliation: Department of Genetic Engineering, Cheongju University, Korea.
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Yoon
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: An
authors.forename: Inn Goo
authors.initials: IG
authors.lastname: Kyeong
authors.forename: Myeong Seon
authors.initials: MS
authors.lastname: Lee
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Kwon
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Kang
mesh_terms: D000818:Animals; D005293:Ferritins / Q000737:chemistry / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006736:Horses; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D016877:Oxidants; D017382:Reactive Oxygen Species; D005293:Ferritins; D006861:Hydrogen Peroxide; D007501:Iron