title: Biological monitoring of a xenomaterial for grafting: an evaluation in critical-size calvarial defects.
abstract: Our purpose was to evaluate the osteoconduction potential of mixed bovine bone (MBB) xenografts as an alternative for bone grafting of critical-size defects in the calvaria of rats. After surgery, in the time intervals of 1, 3, 6, and 9 months, rats were killed and their skulls collected, radiographed and histologically prepared for analysis. The data obtained from histological analysis reported that the particles of MBB did not promote an intense immunological response, evidencing its biocompatibility in rats. Our results clearly showed the interesting evidence that MBB was not completely reabsorbed at 9 months while a small amount of newly formed bone was deposited by osteoprogenitor cells bordering the defect. However, this discrete bone-forming stimulation was unable to regenerate the bone defect. Overall, our results suggest that the properties of MBB are not suitable for stimulating intense bone regeneration in critical bone defects in rats.
authors.affiliation: Department of Endodontics, University of Grande Rio, Duque de Caxias, RJ 25071-202, Brazil.
authors.forename: Thais
authors.initials: T
authors.lastname: Accorsi-Mendonça
authors.forename: Willian Fernando
authors.initials: WF
authors.lastname: Zambuzzi
authors.forename: Clóvis Monteiro
authors.initials: CM
authors.lastname: Bramante
authors.forename: Tânia Mari
authors.initials: TM
authors.lastname: Cestari
authors.forename: Rumio
authors.initials: R
authors.lastname: Taga
authors.forename: Márcia
authors.initials: M
authors.lastname: Sader
authors.forename: Glória Dulce
authors.initials: GD
authors.lastname: de Almeida Soares
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001857:Bone Matrix / Q000601:surgery; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D002417:Cattle; D003094:Collagen / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010316:Particle Size; D016062:Porosity; D051381:Rats; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D003094:Collagen