title: Molecular detection and identification of Rickettsia species in Ixodes pacificus in California.
abstract: We amplified 16S rRNA, gltA, and ompA genes from Ixodes pacificus by polymerase chain reaction. Sequencing, BLAST analysis, and phylogenetic constructions indicated that two Rickettsia phylotypes are present in I. pacificus. While phylotype G021 has high homology to Ixodes scapularis endosymbiotic Rickettsia, phylotype G022 is a deeply branched novel spotted fever group Rickettsia.
authors.affiliation: Department of Biological Sciences, Humboldt State University, Arcata, California, USA.
authors.forename: Jimmy Ninh
authors.initials: JN
authors.lastname: Phan
authors.forename: Casey Roy
authors.initials: CR
authors.lastname: Lu
authors.forename: William Garrett
authors.initials: WG
authors.lastname: Bender
authors.forename: Robert Marion
authors.initials: RM
authors.lastname: Smoak
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002140:California; D030561:Databases, Nucleic Acid; D018884:Ixodes / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012313:RNA; D012336:RNA, Ribosomal, 16S; D012281:Rickettsia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017421:Sequence Analysis
chemical_list: D001425:Bacterial Outer Membrane Proteins; C034477:RNA primers; D012336:RNA, Ribosomal, 16S; C078167:OMPA outer membrane proteins; D012313:RNA