title: In vitro cell uptake of biocompatible magnetite/chitosan nanoparticles with high magnetization: a single-step synthesis approach for in-situ-modified magnetite by amino groups of chitosan.
abstract: Current attempts for synthesizing chitosan-modified magnetite nanoparticles (CS-MNPs) as drug carrier involve use of surfactants, which brings potential cytotoxicity and decrease of saturated magnetization (Ms). To address this, we developed a facile single-step method for synthesizing CS-MNPs. The developed method offers several advantages. No surfactant was involved and the magnetite nanoparticles were in-situ-coated with a chitosan (CS) layer, which endows the CS-MNPs with enhanced physical and chemical properties. The Ms of magnetite was significantly increased (55.5 emu/g) as compared to magnetite nanoparticles modified by surfactants with oxygen ligands, indicating their potential applications as drug-delivery systems (DDSs) where high saturated magnetization (Ms) is preferred. Besides, the amount of CS layer of CS-MNPs was approx. 24 wt%, which makes the synthesized CS-MNPs highly biocompatible as no acute adverse effect of the viability on MG-63 cells was observed at doses up to 0.2 mg/ml. Macropinocytosis is the main endocytic mechanism of MG-63 cells internalize CS-MNPs, which might avoid lysosomal degradation of the CS-MNPs. The synthesized CS-MNPs have potential applications in the fields of targeted drug delivery, separation for purification and immunoassay and magnetic resonance imaging.
authors.affiliation: a Institute for Advanced Ceramics and State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, P. O. Box 433, Harbin 150001, P. R. China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Wang
authors.forename: Baoqiang
authors.initials: B
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Dechang
authors.initials: D
authors.lastname: Jia
authors.forename: Yujie
authors.initials: Y
authors.lastname: Feng
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry*; D004705:Endocytosis; D006801:Humans; D057446:Hydrodynamics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D010100:Oxygen / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D013501:Surface-Active Agents; D048271:Chitosan; D010100:Oxygen