title: Correlation of levels of oncostatin M cytokine in crevicular fluid and serum in periodontal disease.
abstract: OBJECTIVE
  The aim of this study was to measure the level of Oncostatin M (OSM) a gp130 cytokine in the gingival crevicular fluid (GCF) and serum of chronic periodontitis patients and to find any correlation between them before and after periodontal therapy (scaling and root planing, SRP).
  
  
  METHODS
  60 subjects (age 25-50 years) were enrolled into three groups (n=20 per group), group I (healthy), group II (gingivitis) and group III (chronic periodontitis). Group III subjects were followed for 6-8 weeks after the initial periodontal therapy (SRP) as the group IV (after periodontal therapy). Clinical parameters were assessed as gingival index (GI), probing depth (PD), clinical attachment level (CAL), and radiographic evidence of bone loss. GCF and serum levels of OSM were measured by using Enzyme Linked Immunosorbent Assay (ELISA).
  
  
  RESULTS
  It was found that mean OSM levels had been elevated in both the GCF and serum of chronic periodontitis subjects (726.65 +/- 283.56 and 65.59 +/- 12.37 pg mL(-1), respectively) and these levels were decreased proportionally after the periodontal therapy (95.50 +/- 38.85 and 39.98 +/- 16.69 pg mL(-1) respectively). However, OSM was detected in GCF of healthy subjects (66.15 +/- 28.10 pg mL(-1)) and gingivitis-suffering subjects (128.33 +/- 22.96 pg mL(-1)) and was found as below the detectable limit (approximately equal 0.0 pg mL(-1)) in the serum of same subjects. Significant correlation has been found between clinical parameters and GCF-serum levels of OSM.
  
  
  CONCLUSIONS
  Increased OSM level both in the GCF and serum, and the decreased levels after initial periodontal therapy (SRP) may suggest a use as an inflammatory biomarker in the periodontal disease.
authors.affiliation: Department of Periodontics, Government Dental College and Research Institute, Karnataka, India. manojthoratmds@rediffmail.com
authors.forename: ManojKumar
authors.initials: M
authors.lastname: Thorat
authors.forename: A R
authors.initials: AR
authors.lastname: Pradeep
authors.forename: Garima
authors.initials: G
authors.lastname: Garg
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood / Q000378:metabolism* / Q000628:therapy; D012534:Dental Scaling; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000097:blood / Q000378:metabolism / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D053683:Oncostatin M / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D010512:Periodontal Index; D018709:Statistics, Nonparametric
chemical_list: D053683:Oncostatin M