title: Comparative evaluation of a chromogenic agar medium, the modified Hodge test, and a battery of meropenem-inhibitor discs for detection of carbapenemase activity in Enterobacteriaceae.
abstract: Three phenotypic methods (modified Hodge test, chromogenic agar, and meropenem discs combined with specific inhibitors) used for the detection of carbapenemase activity were tested on a panel of characterized Enterobacteriaceae expressing various β-lactamase mechanisms. Overall, the meropenem-plus-inhibitor approach was more sensitive and specific than the other methods, despite its limitation of being unable to detect class D carbapenemases.
authors.affiliation: Ontario Agency for Health Protection and Promotion, Toronto, Ontario, Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Seah
authors.forename: Donald E
authors.initials: DE
authors.lastname: Low
authors.forename: Samir N
authors.initials: SN
authors.lastname: Patel
authors.forename: Roberto G
authors.initials: RG
authors.lastname: Melano
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002863:Chromogenic Compounds / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D004755:Enterobacteriaceae / Q000201:enzymology*; D006801:Humans; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000494:pharmacology*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002863:Chromogenic Compounds; D003470:Culture Media; D013845:Thienamycins; D000362:Agar; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem