title: Post-prandial rise of microvesicles in peripheral blood of healthy human donors.
abstract: BACKGROUND
  Microvesicles isolated from body fluids are membrane - enclosed fragments of cell interior which carry information on the status of the organism. It is yet unclear how metabolism affects the number and composition of microvesicles in isolates from the peripheral blood.
  
  
  OBJECTIVE
  To study the post - prandial effect on microvesicles in isolates from the peripheral blood of 21 healthy donors, in relation to blood cholesterol and blood glucose concentrations.
  
  
  RESULTS
  The average number of microvesicles in the isolates increased 5 hours post - prandially by 52%; the increase was statistically significant (p = 0.01) with the power P = 0.68, while the average total blood cholesterol concentration, average low density lipoprotein cholesterol concentration (LDL-C) and average high density lipoprotein cholesterol concentration (HDL-C) all remained within 2% of their fasting values. We found an 11% increase in triglycerides (p = 0.12) and a 6% decrease in blood glucose (p < 0.01, P = 0.74). The post - prandial number of microvesicles negatively correlated with the post - fasting total cholesterol concentration (r = - 0.46, p = 0.035) while the difference in the number of microvesicles in the isolates between post - prandial and post - fasting states negatively correlated with the respective difference in blood glucose concentration (r = - 0.39, p = 0.05).
  
  
  CONCLUSIONS
  In a population of healthy human subjects the number of microvesicles in isolates from peripheral blood increased in the post - prandial state. The increase in the number of microvesicles was affected by the fasting concentration of cholesterol and correlated with the decrease in blood glucose.
authors.affiliation: Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva ulica 2, Ljubljana, Slovenia.
authors.forename: Vid
authors.initials: V
authors.lastname: Suštar
authors.forename: Apolonija
authors.initials: A
authors.lastname: Bedina-Zavec
authors.forename: Roman
authors.initials: R
authors.lastname: Stukelj
authors.forename: Mojca
authors.initials: M
authors.lastname: Frank
authors.forename: Eva
authors.initials: E
authors.lastname: Ogorevc
authors.forename: Rado
authors.initials: R
authors.lastname: Janša
authors.forename: Keriya
authors.initials: K
authors.lastname: Mam
authors.forename: Peter
authors.initials: P
authors.lastname: Veranič
authors.forename: Veronika
authors.initials: V
authors.lastname: Kralj-Iglič
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002450:Cell Communication; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D011992:Endosomes / Q000378:metabolism*; D005215:Fasting / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D014280:Triglycerides; D002784:Cholesterol