title: A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus.
abstract: To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of genomic DNA tested: males yielded two bands and females a single band. Sequencing of each PCR product confirmed that the 769-bp fragment amplified from DNA samples of both sexes was derived from EIF2S3X, whereas the 546-bp fragment amplified only from male DNA samples was derived from EIF2S3Y. The results indicated that this primer set is useful for sex identification in this species.
authors.affiliation: Graduate School of Life Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Murata
authors.forename: G
authors.initials: G
authors.lastname: Ogura
authors.forename: A
authors.initials: A
authors.lastname: Kuroiwa
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics*; D005260:Female; D008988:Herpestidae / Q000235:genetics*; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012732:Sex Determination Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D017931:DNA Primers