title: Electrochemical biosensor based on nanoporous gold electrode for detection of PML/RARα fusion gene.
abstract: In this study, a kind of nanoporous gold electrode (NPG) prepared with repetitive square-wave oxidation reduction cycle (SWORC) was reported. The active surface area of the proposed NPG electrode was 9.9 times larger than that of a bare flat one characterized by cyclic voltammetry (CV). An electrochemical DNA biosensor based on NPG electrode was fabricated for detection of promyelocytic leukemia/retinoic acid receptor α (PML/RARα) fusion gene in acute promyelocytic leukemia (APL) by using Methylene Blue (MB) as an electroactive indicator. Differential pulse voltammetry (DPV) was employed to monitor the hybridization reaction on the probe modified electrode. The decrease of the peak current of MB was observed upon hybridization of the probe with target DNA. The results indicated that the peak current was linear with the concentration of complementary strand in the range of 60 pM to 220 pM with a detection limit of 6.7 pM. This new biosensor exhibited excellent sensitivity and selectivity and had been used for an assay of PCR real sample with a satisfactory result.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Guangxian
authors.initials: G
authors.lastname: Zhong
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China; Fujian Institute of Hematology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou 350000, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.affiliation: Fujian Key Lab of Medical Instrumentation and Pharmaceutical Technology, Fuzhou University, Fuzhou, Fujian 350002, China; College of Electrical Engineering and Automation, Fuzhou University, Fuzhou 350000, China.
authors.forename: Xuhai
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Lian
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China.
authors.forename: Qicai
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China; Fujian Institute of Hematology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou 350000, China.
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Fujian Key Lab of Medical Instrumentation and Pharmaceutical Technology, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Min
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D055664:Electrochemical Techniques; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D015514:Oncogene Proteins, Fusion / Q000737:chemistry / Q000302:isolation & purification*; D010084:Oxidation-Reduction
chemical_list: D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D004247:DNA