title: Real-time PCR detection of Parvicapsula pseudobranchicola (Myxozoa: Myxosporea) in wild salmonids in Norway.
abstract: The myxozoan genus Parvicapsula contains 14 species infecting fish, some of which are known to cause severe disease in farmed and wild salmonids. Parvicapsula pseudobranchicola infections were first reported from seawater-reared Atlantic salmon, Salmo salar, in Norway in 2002 and have since then been an increasing problem. The present study describes a Taqman real-time PCR assay for specific detection of P. pseudobranchicola. The Taqman assay targets the 18S rRNA gene of P. pseudobranchicola and is able to detect as few as ten copies of the target sequence. Using the described assay, P. pseudobranchicola was detected in both farmed and wild salmonids, indicating that wild Atlantic salmon, sea trout, Salmo trutta, and Arctic char, Salvelinus alpinus, may be natural hosts of the parasite. Parvicapsula pseudobranchicola was found in samples from wild salmonids in the far south and the far north of Norway, displaying a wide geographic range of the parasite. Farmed salmonids showed P. pseudobranchicola infection levels many folds higher than that observed for wild sea trout, indicating that farmed Atlantic salmon are subjected to an elevated infection pressure compared with wild salmonids.
authors.affiliation: Norwegian Veterinary Institute, Oslo, Norway. anders.jorgensen@vetinst.no
authors.forename: A
authors.initials: A
authors.lastname: Jørgensen
authors.forename: A
authors.initials: A
authors.lastname: Nylund
authors.forename: V
authors.initials: V
authors.lastname: Nikolaisen
authors.forename: S
authors.initials: S
authors.lastname: Alexandersen
authors.forename: E
authors.initials: E
authors.lastname: Karlsbakk
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000175:diagnosis* / Q000453:epidemiology; D005398:Fisheries; D005399:Fishes; D055764:Myxozoa / Q000302:isolation & purification*; D009664:Norway; D010273:Parasitic Diseases, Animal / Q000175:diagnosis* / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary*; D012487:Salmonidae / Q000469:parasitology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000662:veterinary