title: An ARMS-based technique for sex determination of red panda (Ailurus fulgens).
abstract: Molecular sexing is a key component in the investigation of wild populations. In this study, we developed a fast, accurate and reliable amplification refractory mutation system (ARMS) technique for sex determination of red panda based on the exon 4 of the ZFX/ZFY gene. The amplicons were distinguished simply by agarose gel electrophoresis, exhibiting one fragment in females (X: 300 bp) and two in males (X: 300 bp, Y: 166 bp). Robustness of this ARMS system was confirmed by testing both 43 captive red pandas using DNA samples with known-sex and 10 wild red pandas using faecal DNA samples with unknown sex.
authors.affiliation: Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Sichuan, China.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fujun
authors.initials: F
authors.lastname: Shen
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhang
authors.forename: Bisong
authors.initials: B
authors.lastname: Yue
mesh_terms: D053822:Ailuridae / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005091:Exons; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D012732:Sex Determination Analysis / Q000379:methods*