title: [Hypoxia induces fibrosis of corpus cavernosum smooth muscle in SD rats].
abstract: OBJECTIVE
  To explore the effect of hypoxia on the fibrosis of corpus cavernosum smooth muscle cells (CCSMC) in SD rats.
  
  
  METHODS
  CCSMCs were cultured in vitro, identified by immunohistochemistry, and then exposed to hypoxia at the concentration of 1% O2 for 12, 24, 48 and 72 hours. Those exposed to normal oxygen concentration for the corresponding lengths of time were used as the control. The relative expressions of TGF-131, type I collagen and type DI collagen were determined by RT-PCR.
  
  
  RESULTS
  The in vitro cultured CCSMCs grew well, and the anti-a-smooth muscle actin monoclonal antibodies were positive on immunohistochemical staining. The relative expression levels of TGF-beta1, type I collagen and type mI collagen were positively correlated with the time of hypoxia interference within 48 hours, and did not increase further with prolonged exposure.
  
  
  CONCLUSIONS
  When exposed to hypoxia, the relative expressions of TGF-beta1, type I collagen and type mI collagen in the CCSMCs of SD rats increased with the length of time, and reached the peak at 48 hours. Hypoxia can cause fibrosis of CCSMCs in SD rats.
authors.affiliation: Department of Urology, The Second Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310005, China. lbd168@126.com
authors.forename: Bo-dong
authors.initials: BD
authors.lastname: Lü
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Nian
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Shi-geng
authors.initials: SG
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Geng
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Shi-tao
authors.initials: ST
authors.lastname: Chen
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005109:Extracellular Matrix; D005355:Fibrosis; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology* / Q000473:pathology*; D010100:Oxygen / Q000378:metabolism*; D010413:Penis / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D024042:Collagen Type I; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D010100:Oxygen