title: [Differentiation of genetic variants of Crimean-Congo hemorrhagic fever virus].
abstract: The paper describes a simple, rapid screening of samples potentially containing Crimean-Congo hemorrhagic fever (CCHF) virus strains, by applying the restriction analysis of amplicones, for the differentiation of CCHF virus genotypes that are characteristic of Europe from virus biovariants uncharacteristic of this area, this technique requiring no sequence at the first stage. For this screening, the authors propose to use the PCR fragment of CCHF L segment that comprises a variable region, as well as Alul and Haelll restriction endonucleases. The screening scheme proposed for samples potentially containing CCHF virus may aid investigators to monitor in order to detect uncharacteristic genotypic virus variants in the Russian Federation and other European countries.
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: S S
authors.initials: SS
authors.lastname: Seregin
authors.forename: V S
authors.initials: VS
authors.lastname: Petrov
authors.forename: G I
authors.initials: GI
authors.lastname: Tiunnikov
authors.forename: N V
authors.initials: NV
authors.lastname: Iakimenko
authors.forename: A N
authors.initials: AN
authors.lastname: Sergeev
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D004262:DNA Restriction Enzymes; D014644:Genetic Variation; D016679:Genome, Viral; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D058974:Phylogeography; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D004262:DNA Restriction Enzymes