title: Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction.
abstract: Pharmaceuticals residues in the environment have become a growing scientific interest worldwide. In the light of the possible harmful effects of tobramycin, a rapid and sensitive analytical method for determination of tobramycin in soil was developed. The extraction and purification methods, derivatization conditions, and chromatographic conditions in the determination of tobramycin in soil have been fully investigated. Extraction was carried out by a combination of vortex mixer and ultrasonic oscillation using acetone/water as the extraction agent. The extract was concentrated to 1 mL and passed through the C(18) SPE cartridge rinsed with water (3 mL), methanol (3 mL). The derivatization procedure was followed by the reaction of tobramycin with 4-Chloro-3,5-dinitrobenzotrifluoride at 60°C for 10 min in pH 9.0 H(3)BO(3)-Na(2)B(4)O(7) medium. The labeled tobramycin was determined by high performance liquid chromatography at 245 nm. Separation was accomplished within 15 min in gradient elution mode with trifluoroacetic acid in mobile phase as ion-pair reagent. The correlation coefficient for the method was 0.9999 in concentrations ranging from 0.10 to 100.0 μg/g. The limit of detection was 0.02 μg/g for tobramycin in soil at a signal-to-noise ratio of 3. The calculated recoveries of the proposed method were from 78.0 to 91.0% and RSDs were 3.38-9.79% in the application to the quantitative determination of tobramycin in all types of soil. The method will help to establish adequate monitoring of tobramycin residue in soil and make the contribution to environmental behavior evaluation.
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors.forename: Shun
authors.initials: S
authors.lastname: He
authors.forename: Qiyou
authors.initials: Q
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuncong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Laixin
authors.initials: L
authors.lastname: Luo
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Li
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Cao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D004355:Drug Stability; D005464:Fluorobenzenes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D009574:Nitro Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D013010:Sonication; D013696:Temperature; D014031:Tobramycin / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D005464:Fluorobenzenes; D009574:Nitro Compounds; D012987:Soil; D012989:Soil Pollutants; C539305:4-chloro-3,5-dinitrobenzotrifluoride; D014031:Tobramycin