title: [Expression and significance of ENPP1, TNAP and ANK proteins in the degeneration of endplate chondrocytes in rats].
abstract: OBJECTIVE
  To study the expression of the pyrophosphoric acid loop's key enzymes (ENPP1, TNAP and ANK proteins) in the degeneration of rat endplate chondrocyte.
  
  
  METHODS
  The method of enzyme digestion plus natural subculture was employed. And the primary to forth-generation cells were collected. Western blot was used to analyze the expression of ENPP1, TNAP and ANK proteins in each generation of endplate chondrocytes.
  
  
  RESULTS
  The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually and that of the third and forth-generation decreased.
  
  
  CONCLUSIONS
  While studying the correlation between the degeneration of rat endplate chondrocytes and pyrophosphoric acid loop, it is more significant to choose the cells before the third generation. Meanwhile it is possible to lower the incidence of intervertebral discs degeneration by controlling the expression of the above-mentioned proteins in endplate cartilage.
authors.affiliation: Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China. xuhg@medmail.com.cn
authors.forename: Hong-guang
authors.initials: HG
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D007403:Intervertebral Disc / Q000378:metabolism* / Q000473:pathology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; C065962:ectonucleotide pyrophosphatase phosphodiesterase 1; D011755:Pyrophosphatases