title: Calcium phosphate mediated gene transfer into established cell lines.
abstract: DNA transfection is one of the most important techniques in molecular genetics. It is this technique that has made possible the dissection of complex eukaryotic genes and the characterization of the function of their components (1-7) as well as the isolation of particular genes on the basis of their expression in cultured cells (8-11).
authors.affiliation: Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okayama
authors.forename: C
authors.initials: C
authors.lastname: Chen