title: Toxicological potential of mycotoxin MT81 and its benzoylated derivative on testicular spermatogenesis and steroidogenesis in mature male Wistar albino rats.
abstract: Mycotoxin MT81 was isolated, purified, and identified from a fungal strain of Penicillium nigricans. It is a CNS depressant, hyperglycemic agent and produces massive bone marrow depression, hepatotoxicity, and nephrotoxicity. Its benzolylated analog (benzoylated-MT81) was synthesized in our laboratory having a LD₅₀ value of 87.1 mg/kg body weight in mice. This study was designed to assess the toxicological effects of mycotoxin MT81 and its analog on testicular spermatogenesis and steroidogenesis in mature albino rats. The sperm count and percentage of motile sperm were decreased markedly in MT81- and benzoylated-MT81-treated rats. The body weight and the weight of testis were reduced, whereas weight of adrenal gland was increased in a dose-dependent manner in the toxin-treated rats. MT81 and its derivative caused accumulation of ascorbic acid and total cholesterol in the testis and reduction in the activities of Δ⁵-3β-hydroxysteroid dehydrogenase (Δ⁵-3β-HSD) and glucose-6-phosphate dehydrogenase (G-6-P-D), whereas the ascorbic acid and cholesterol content of adrenal gland were decreased and enzyme activities were elevated. This experiment suggests that MT81 and benzoylated-MT81 both produce inhibition of testicular spermatogenesis and steroidogenesis but increase adrenal steroidogenesis and ultimately sterility of male rats.
authors.affiliation: Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, West Bengal, India.
authors.forename: Sujata Maiti
authors.initials: SM
authors.lastname: Choudhury
authors.forename: Malaya
authors.initials: M
authors.lastname: Gupta
authors.forename: Upal Kanti
authors.initials: UK
authors.lastname: Majumder
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000311:Adrenal Glands / Q000187:drug effects / Q000473:pathology; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry / Q000633:toxicity*; D001205:Ascorbic Acid / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D009183:Mycotoxins / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000378:metabolism; D018675:Toxicity Tests
chemical_list: D000880:Anthraquinones; D009183:Mycotoxins; D013739:Testosterone; C076604:MT81 mycotoxin; D002784:Cholesterol; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001205:Ascorbic Acid