title: [Development of an ultrasensitive mismatch tolerant PCR assay for the qualitative and quantitative determination of HIV-1 RNA].
abstract: A real-time fluorescent polymerase chain reaction was used to develop a RealBest HIV RNA kit that was clinically suitable for the detection of HIV-1 RNA and for the estimation of virus load in plasma and serum samples. Due to the selection of a highly conserved target region and to the experimental study of the impact of different primer-template and probe-template mismatches on RT-PCR with subsequent selection of the optimum oligonucleotide set, the developed assay can detect and measure the concentration of all subtypes of HIV-1, group M. The assay provides a high reproducibility and sensitivity and a wide dynamic range of virus loads (20 to 10 million IU/ml of plasma or serum).
authors.forename: M A
authors.initials: MA
authors.lastname: Prasolova
authors.forename: M K
authors.initials: MK
authors.lastname: Ivanov
authors.forename: G M
authors.initials: GM
authors.lastname: Dymshits
mesh_terms: D020137:Base Pair Mismatch; D017931:DNA Primers / Q000737:chemistry; D005453:Fluorescence; D014644:Genetic Variation; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D011933:Reagent Kits, Diagnostic / Q000821:virology*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D017931:DNA Primers; D009841:Oligonucleotides; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic