title: Nasal septum-derived multipotent progenitors: a potent source for stem cell-based regenerative medicine.
abstract: Thus far, autologous adult stem cells have attracted great attention for clinical purposes. In this study, we aimed at identifying and comprehensively characterizing a subpopulation of multipotent cells within human nasal septal cartilage. We also conducted a comparative investigation with other well-established stem cells such as bone marrow-mesenchymal stem cells, adipose tissue-mesenchymal stem cells, and unrestricted somatic stem cells. The isolated clonal population was characterized using immunofluorescence, flow cytometry, reverse transcriptase, and real-time polymerase chain reaction. Nasal septal progenitors (NSP) expressed critical pluripotency and mesoectodermal stem cell markers. They also shared many characteristics with MSC in expression of CD90, CD105, CD106, CD166, and HLA-ABC and lack of expression of CD34, CD45, and HLA-DR. NSP distinctly presented CD133 (Prominin-1). These cells could proliferate rapidly in vitro with a higher clonogenic potential and showed a longer lifespan than other studied cells. This population bears some other multipotent properties in showing a high capacity to be differentiated into other lineages including chondrocytes, osteocytes, and neural-like cell types. Another strong/positive feature of this population was their ability to be safely expanded ex vivo with no susceptibility to chromosomal abnormality or tumorigenicity both in vitro and in vivo. In conclusion, NSP could be considered as an alternative autologous cell source that can bring them to the top of therapeutic applications.
authors.affiliation: Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Kabiri
authors.forename: Naser
authors.initials: N
authors.lastname: Ahmadbeigi
authors.forename: Saeed Oraee
authors.initials: SO
authors.lastname: Yazdani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mojtahed
authors.forename: Saeid
authors.initials: S
authors.lastname: Amanpour
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D016922:Cellular Senescence; D002999:Clone Cells; D015925:Cryopreservation; D005455:Fluorescent Antibody Technique; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009300:Nasal Septum / Q000166:cytology*; D044968:Regenerative Medicine / Q000379:methods*; D013997:Time Factors; D055815:Young Adult; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001616:beta-Galactosidase