title: Identification and quantification of coumarins in Peucedanum ostruthium (L.) Koch by HPLC-DAD and HPLC-DAD-MS.
abstract: The rhizomes of Peucedanum ostruthium (L.) Koch (masterwort) are traditionally used in the alpine region as ingredient of liqueurs and bitters, and as a herbal drug. A sensitive and specific high-performance liquid chromatography-diode-array detection-mass spectrometry (HPLC-DAD-MS) method has been developed for the simultaneous identification and quantification of its main coumarins, oxypeucedanin hydrate, oxypeucedanin, ostruthol, imperatorin, osthole, isoimperatorin, and ostruthin. Fast HPLC separation could be achieved on an Acclaim C18 column (150 mm × 2.1 mm i.d., 3 μm) using a mobile phase gradient of acetonitrile-water modified with 0.01% acetic acid. The quantification by HPLC-DAD was performed with imperatorin as external standard and validated to demonstrate selectivity, linearity, precision, and accuracy. The content of the main coumarins was quantitated in various batches of commercial and field-collected rhizomes of Peucedanum ostruthium, as well as in beverages prepared thereof.
authors.affiliation: Department of Pharmacognosy, University of Vienna, Vienna, Austria.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Vogl
authors.forename: Martin
authors.initials: M
authors.lastname: Zehl
authors.forename: Paolo
authors.initials: P
authors.lastname: Picker
authors.forename: Ernst
authors.initials: E
authors.lastname: Urban
authors.forename: Christoph
authors.initials: C
authors.lastname: Wawrosch
authors.forename: Gottfried
authors.initials: G
authors.lastname: Reznicek
authors.forename: Johannes
authors.initials: J
authors.lastname: Saukel
authors.forename: Brigitte
authors.initials: B
authors.lastname: Kopp
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003374:Coumarins / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D003374:Coumarins; D010936:Plant Extracts