title: Phenol tolerance, changes of antioxidative enzymes and cellular damage in transgenic tobacco hairy roots colonized by arbuscular mycorrhizal fungi.
abstract: Phytoremediation has been recognized as a cheap and eco-friendly technology which could be used for the remediation of organic pollutants, such as phenolic compounds. Besides, the extent to which plants react to environmental pollution might depend on rhizosphere processes such as mycorrhizal symbiosis. In the present work, phenol tolerance of transgenic tobacco hairy roots (HR), namely TPX1, colonized with an arbuscular mycorrhizal fungus (AMF) was studied. However, the question is whether AMF symbiosis can moderate adverse effects of phenol to the plant tissues. Thus, the antioxidative response as well as parameters of oxidative damage, like malondialdehyde (MDA) content, were determined. Antioxidative enzymes such as peroxidase, superoxide dismutase, ascorbate peroxidase were higher in TPX1 HR colonized with AMF, compared to wild type HR colonized by AMF, in the presence of increasing concentrations of the pollutant. Besides, MDA levels remained unaltered in TPX1 HR associated with AMF treated with the xenobiotic. These results, suggested that this culture could tolerate phenol and moreover, it has an efficient protective mechanism against phenol-induced oxidative damage, which is of great importance in the selection of species with remediation capacities. Thus, transgenic HR colonized with AMF could be considered as an interesting model system to study different processes which play a key role in the phytoremediation of organic pollutants.
authors.affiliation: Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, CP 5800 Río Cuarto, Córdoba, Argentina.
authors.forename: Sabrina G
authors.initials: SG
authors.lastname: Ibáñez
authors.forename: María I
authors.initials: MI
authors.lastname: Medina
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Agostini
mesh_terms: D000222:Adaptation, Physiological; D060387:Ascorbate Peroxidases; D015227:Lipid Peroxidation / Q000187:drug effects; D038821:Mycorrhizae / Q000187:drug effects* / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D019800:Phenol / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology; D012989:Soil Pollutants / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D013559:Symbiosis; D014026:Nicotiana / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology
chemical_list: D012989:Soil Pollutants; D019800:Phenol; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D009195:Peroxidase; D013482:Superoxide Dismutase