title: [One example of false negative hepatitis B surface antigen (EIA) result due to variant S area strain and reagment reactiveness related to hepatitis B surface antigen].
abstract: The presence in serum of the Hepatitis B surface antigen (HBsAg), the outer envelope of the hepatitis B virus (HBV), indicates viral infection, used in laboratory tests to confirm this. We report a case of discrepancy among HBsAg test results detected between measurements in a subject with HB infection. Gene analysis demonstrated several S region gene mutations, not detected previously. We tested 12 measurements e.g., EIA, CLIA, CLEIA, F-EIA, MAT, and IC for whether they could detect our subject's HBsAg and found that it was not recognized by a method using only a single monoclonal antibody to detect HBsAg in two detection processes, in contrast to the 11 other measurements, which used two different antibodies. This case shows that amino acid substitution may cause a false negative result for HBsAg. Gene mutations known to occur in HBV, should thus trigger an awareness of the need to keep in mind that false negative results can happen in case such as ours.
authors.affiliation: Central Clinical Laboratory, Shimane University Hospital.
authors.forename: Chikashi
authors.initials: C
authors.lastname: Matsuda
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Moriyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Taketani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shibata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nagai
mesh_terms: D000368:Aged; D005188:False Negative Reactions; D006509:Hepatitis B / Q000175:diagnosis; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D009154:Mutation
chemical_list: D006514:Hepatitis B Surface Antigens; D007202:Indicators and Reagents