title: An integrated genomic approach to the assessment and treatment of acute myeloid leukemia.
abstract: Traditionally, new scientific advances have been applied quickly to the leukemias based on the ease with which relatively pure samples of malignant cells can be obtained. Currently, our arsenal of approaches used to characterize an individual's acute myeloid leukemia (AML) combines hematopathologic evaluation, flow cytometry, cytogenetic analysis, and molecular studies focused on a few key genes. The advent of high-throughput methods capable of full-genome evaluation presents new options for a revolutionary change in the way we diagnose, characterize, and treat AML. Next-generation DNA sequencing techniques allow full sequencing of a cancer genome or transcriptome, with the hope that this will be affordable for routine clinical care within the decade. Microarray-based testing will define gene and miRNA expression, DNA methylation patterns, chromosomal imbalances, and predisposition to disease and chemosensitivity. The vision for the future entails an integrated and automated approach to these analyses, bringing the possibility of formulating an individualized treatment plan within days of a patient's initial presentation. With these expectations comes the hope that such an approach will lead to decreased toxicities and prolonged survival for patients.
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, IL 60637, USA. lgodley@medicine
authors.forename: Lucy A
authors.initials: LA
authors.lastname: Godley
authors.forename: John
authors.initials: J
authors.lastname: Cunningham
authors.forename: M Eileen
authors.initials: ME
authors.lastname: Dolan
authors.forename: R Stephanie
authors.initials: RS
authors.lastname: Huang
authors.forename: Sandeep
authors.initials: S
authors.lastname: Gurbuxani
authors.forename: Megan E
authors.initials: ME
authors.lastname: McNerney
authors.forename: Richard A
authors.initials: RA
authors.lastname: Larson
authors.forename: Hoyee
authors.initials: H
authors.lastname: Leong
authors.forename: Yves
authors.initials: Y
authors.lastname: Lussier
authors.forename: Kenan
authors.initials: K
authors.lastname: Onel
authors.forename: Olatoyosi
authors.initials: O
authors.lastname: Odenike
authors.forename: Wendy
authors.initials: W
authors.lastname: Stock
authors.forename: Kevin P
authors.initials: KP
authors.lastname: White
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Le Beau
mesh_terms: D019175:DNA Methylation; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide