title: Cell-based electrochemical biosensors for water quality assessment.
abstract: During recent decades, extensive industrialisation and farming associated with improper waste management policies have led to the release of a wide range of toxic compounds into aquatic ecosystems, causing a rapid decrease of world freshwater resources and thus requiring urgent implementation of suitable legislation to define water remediation and protection strategies. In Europe, the Water Framework Directive aims to restore good qualitative and quantitative status to all water bodies by 2015. To achieve that, extensive monitoring programmes will be required, calling for rapid, reliable and cost-effective analytical methods for monitoring and toxicological impact assessment of water pollutants. In this context, whole cell biosensors appear as excellent alternatives to or techniques complementary to conventional chemical methods. Cells are easy to cultivate and manipulate, host many enzymes able to catalyse a wide range of biological reactions and can be coupled to various types of transducers. In addition, they are able to provide information about the bioavailability and the toxicity of the pollutants towards eukaryotic or prokaryotic cells. In this article, we present an overview of the use of whole cells, mainly bacteria, yeasts and algae, as sensing elements in electrochemical biosensors with respect to their practical applications in water quality monitoring, with particular emphasis on new trends and future perspectives. In contrast to optical detection, electrochemical transduction is not sensitive to light, can be used for analysis of turbid samples and does not require labelling. In some cases, it is also possible to achieve higher selectivities, even without cell modification, by operating at specific potentials where interferences are limited.
authors.affiliation: University of Lyon, Laboratoire des Sciences Analytiques, UMR, CNRS, CPE, Villeurbanne, France. florence.lagarde@univ-lyon1.fr
authors.forename: Florence
authors.initials: F
authors.lastname: Lagarde
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic-Renault
mesh_terms: D001419:Bacteria / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D002477:Cells / Q000378:metabolism*; D055664:Electrochemical Techniques; D014873:Water Pollutants / Q000032:analysis* / Q000633:toxicity; D014881:Water Supply / Q000592:standards
chemical_list: D014873:Water Pollutants