title: Kinetic microplate assay for determining immobilized antimicrobial peptide activity.
abstract: Antimicrobial peptide immobilization onto surfaces is of great interest, although characterization of activity can be problematic. The kinetic microplate method described here determines the minimum bactericidal concentration (MBC) of immobilized antimicrobial peptides through a combination and modification of traditional solution assays, overcoming the difficulties of working with a solid substrate. The technique enables rapid, accurate evaluation of immobilized peptide lytic behavior, elucidating both dose- and time-dependent activity at multiple concentrations. Furthermore, the method yields information regarding sublethal concentrations not realized in the traditional assays.
authors.affiliation: Biological Science and Technology Team, Warfighter Science Technology and Applied Research Directorate, U.S. Army Natick Soldier Research, Development & Engineering Center, MA 01760-5020, USA. steven.arcidiacono@us.army.mil
authors.forename: Steven
authors.initials: S
authors.lastname: Arcidiacono
authors.forename: Alexa M
authors.initials: AM
authors.lastname: Meehan
authors.forename: Romy
authors.initials: R
authors.lastname: Kirby
authors.forename: Jason W
authors.initials: JW
authors.lastname: Soares
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D010455:Peptides