title: Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana.
abstract: Deep sequencing of the polyadenylated transcriptome of a wild plant of Hardenbergia comptoniana was used to distinguish with high support complete genome sequences of two distinct isolates of Hardenbergia mosaic virus (HarMV) co-infecting it. Isolates shared 82.0% nucleotide (nt) and 86.7% amino acid (aa) identity. Isolate 57.1 (9,628 nt) had a deletion of 17 contiguous aa within the conserved core region of the coat protein (CP), while isolate 57.2 (9,675 nt) had a wild-type CP. Overall, HarMV genomes accounted for 10.69% of total transcripts sequenced, and the mutant virus genome was 23.1% more highly expressed than the wild-type genome.
authors.affiliation: Plant Virus Section, Western Australian State Agricultural Biotechnology Centre, School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA, 6150, Australia. s.wylie@murdoch.edu.au
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wylie
authors.forename: Michael G K
authors.initials: MG
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D007887:Fabaceae / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016415:Sequence Alignment