title: [The 2009 pandemic influenza in Russia. I. Diagnosis and molecular biological characteristics of the virus].
abstract: The analysis of 1558 clinical samples revealed influenza virus A(H1N1v) RNA in 339 patients with influenza and 163 fatal cases,which was made in May to December 2009. Data on the antigenic properties of more than 250 of pandemic virus strains isolated at the Research Institute of Influenza and the molecular genetic characteristics of 31 strains are presented. All the test isolates were found to have the S203 substitution in hemagglutinin, which was characteristic of one of 5 minor genome A(H1N1v) virus variants found in the United States and Mexico in 2009. All the test strains contain the S31N substitution in the M2 protein, which determines viral resistance to adamantine, and have no H275Y substitution in neuraminidase, which determines oseltamivir resistance. The substitution of amino acid residue of Asp to Gly at position 222 of HA was found in 8 (73%) of 11 isolates from postmortem lung and trachea samples and in 2 (10%) of 20 isolates from nasopharyngeal swabs. The determination of the pathogenic role of this substitution calls for further investigations.
authors.forename: O I
authors.initials: OI
authors.lastname: Kiselev
authors.forename: A B
authors.initials: AB
authors.lastname: Komissarov
authors.forename: M A
authors.initials: MA
authors.lastname: Stukova
authors.forename: Zh V
authors.initials: ZhV
authors.lastname: Buzitskaia
authors.forename: M M
authors.initials: MM
authors.lastname: Pisareva
authors.forename: E A
authors.initials: EA
authors.lastname: Elpaeva
authors.forename: D M
authors.initials: DM
authors.lastname: Danilenko
authors.forename: N I
authors.initials: NI
authors.lastname: Konovalova
authors.forename: T M
authors.initials: TM
authors.lastname: Gudkova
authors.forename: V A
authors.initials: VA
authors.lastname: Grigor'eva
authors.forename: T S
authors.initials: TS
authors.lastname: Smirnova
authors.forename: A V
authors.initials: AV
authors.lastname: Slita
authors.forename: E A
authors.initials: EA
authors.lastname: Romanovskaia-Roman'ko
authors.forename: L M
authors.initials: LM
authors.lastname: Tsybalova
authors.forename: A A
authors.initials: AA
authors.lastname: Sominina
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Eropkin
authors.forename: M P
authors.initials: MP
authors.lastname: Grudinin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000547:Amantadine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D019943:Amino Acid Substitution / Q000187:drug effects; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D002642:Chick Embryo; D002648:Child; D002675:Child, Preschool; D024882:Drug Resistance, Viral / Q000187:drug effects / Q000235:genetics; D006388:Hemagglutinins / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis / Q000188:drug therapy / Q000401:mortality / Q000821:virology*; D008168:Lung / Q000821:virology; D008800:Mexico; D008875:Middle Aged; D009026:Mortality; D009305:Nasopharynx / Q000821:virology; D009439:Neuraminidase / Q000235:genetics*; D053139:Oseltamivir / Q000494:pharmacology / Q000627:therapeutic use; D058873:Pandemics; D010802:Phylogeny; D016865:Reassortant Viruses / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012426:Russia; D014132:Trachea / Q000821:virology; D014481:United States; D014763:Viral Matrix Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D055815:Young Adult; D000086082:Viroporin Proteins
chemical_list: D000547:Amantadine; D000998:Antiviral Agents; D006388:Hemagglutinins; D009439:Neuraminidase; D053139:Oseltamivir; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins; C005660:adamantanine