title: The comparative performance of the single intradermal test and the single intradermal comparative tuberculin test in Irish cattle, using tuberculin PPD combinations of differing potencies.
abstract: In national bovine tuberculosis (BTB) control programmes, testing is generally conducted using a single source of bovine purified protein derivative (PPD) tuberculin. Alternative tuberculin sources should be identified as part of a broad risk management strategy as problems of supply or quality cannot be discounted. This study was conducted to compare the impact of different potencies of a single bovine PPD tuberculin on the field performance of the single intradermal comparative tuberculin test (SICTT) and single intradermal test (SIT). Three trial potencies of bovine PPD tuberculin, as assayed in naturally infected bovines, namely, low (1192IU/dose), normal (6184IU/dose) and high (12,554IU/dose) were used. Three SICTTs (using) were conducted on 2102 animals. Test results were compared based on reactor-status and changes in skin-thickness at the bovine tuberculin injection site. There was a significant difference in the number of reactors detected using the high and low potency tuberculins. In the SICTT, high and low potency tuberculin detected 40% more and 50% fewer reactors, respectively, than normal potency tuberculin. Furthermore, use of the low potency tuberculin in the SICTT failed to detect 20% of 35 animals with visible lesions, and in the SIT 11% of the visible lesion animals would have been classified as negative. Tuberculin potency is critical to the performance of both the SICTT and SIT. Tuberculin of different potencies will affect reactor disclosure rates, confounding between-year or between-country comparisons. Independent checks of tuberculin potency are an important aspect of quality control in national BTB control programmes.
authors.affiliation: Department of Agriculture Fisheries and Food, Kildare St., Dublin 2, Ireland. Electronic address: margaret.good@agriculture.gov.ie.
authors.forename: M
authors.initials: M
authors.lastname: Good
authors.affiliation: Centre for Veterinary Epidemiology and Risk Analysis, UCD School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: T A
authors.initials: TA
authors.lastname: Clegg
authors.affiliation: Department of Agriculture Fisheries and Food, Central Veterinary Research Laboratory, Backweston Laboratory Complex, Celbridge, Co. Kildare, Ireland.
authors.forename: E
authors.initials: E
authors.lastname: Costello
authors.affiliation: Centre for Veterinary Epidemiology and Risk Analysis, UCD School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: S J
authors.initials: SJ
authors.lastname: More
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D006168:Guinea Pigs; D007202:Indicators and Reagents / Q000008:administration & dosage; D016015:Logistic Models; D008297:Male; D012680:Sensitivity and Specificity; D014373:Tuberculin / Q000008:administration & dosage; D014374:Tuberculin Test / Q000379:methods / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
chemical_list: D007202:Indicators and Reagents; D014373:Tuberculin