title: Straight and branched (ω-1)-hydroxylated very long chain fatty acids are components of Bradyrhizobium lipid A.
abstract: Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (common broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland. adam.choma@poczta.umcs.lublin.pl
authors.forename: Adam
authors.initials: A
authors.lastname: Choma
authors.forename: Iwona
authors.initials: I
authors.lastname: Komaniecka
mesh_terms: D020369:Bradyrhizobium / Q000737:chemistry*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000737:chemistry*; D008050:Lipid A / Q000737:chemistry*; D013058:Mass Spectrometry
chemical_list: D005227:Fatty Acids; D008050:Lipid A