title: Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.
abstract: Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan. mat@nias.affrc.go.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sakai
authors.forename: Naoki
authors.initials: N
authors.lastname: Amano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanamori
authors.forename: Kanako
authors.initials: K
authors.lastname: Kurita
authors.forename: Ari
authors.initials: A
authors.lastname: Kikuta
authors.forename: Kozue
authors.initials: K
authors.lastname: Kamiya
authors.forename: Mayu
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ikawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fujii
authors.forename: Kiyosumi
authors.initials: K
authors.lastname: Hori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Itoh
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sato
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D018745:Genome, Plant / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant