title: Bacterial identification by 16S rRNA gene PCR-hybridization as a supplement to negative culture results.
abstract: PCR-hybridization was compared to culture methods for evaluating suspected blood infections. A total of 231 clinical samples from blood culture bottles that were flagged positive by the BacT/Alert system or were negative 1 week after inoculation were tested. When the PCR-hybridization and culture method results were compared, the positive and negative concordance rates were 99.2% (122/123) and 89.5% (94/105), respectively. Of the negative blood cultures, 10.5% (11/105) were positive by PCR-hybridization. Supplemental testing of negative blood cultures may identify bacterial pathogens that are undetectable by culture methods.
authors.affiliation: Division of Infectious Disease, Children's Hospital Los Angeles, and Metic Transplantation Laboratory,Keck School of Medicine, University of Southern California, Los Angeles, California, USA. kmatsuda@usc.edu.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Matsuda
authors.forename: Kosuke K
authors.initials: KK
authors.lastname: Iwaki
authors.forename: Jean
authors.initials: J
authors.lastname: Garcia-Gomez
authors.forename: Jill
authors.initials: J
authors.lastname: Hoffman
authors.forename: Clark B
authors.initials: CB
authors.lastname: Inderlied
authors.forename: Wilbert H
authors.initials: WH
authors.lastname: Mason
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Iwaki
mesh_terms: D000293:Adolescent; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S