title: Evaluation of DRB1 high resolution typing by a new SSO-based Luminex method.
abstract: HLA testing is an essential part of the process to identify a donor who may be a good match for the patients who need haematopoietic stem cells from bone marrow, peripheral blood or cord blood and the DNA typing in high resolution is now recommended as the Scientific Societies also describe in their standards. Recently the new PCR-Luminex HLA typing method, based on the reverse sequence specific oligonucleotide probes coupled with a microsphere beads in an array platform, has been well established. We report the data from 146 samples previously typed to a four digits level and used to evaluate the accuracy, sensitivity and performance of the new high definition DRB1 by PCR-Luminex kit. One hundred and forty-six samples from unrelated healthy donors, haematological patients or external proficiency tests were used in this study. The Luminex high definition DRB1 typing represents a versatile method and may be easily introduced in the routine, particularly when the technical team has already acquired experience on the technique. Only few HLA allelic combinations need an additional typing by PCR-SSP or SBT to solve the ambiguous results thus reducing the time necessary to produce a final report.
authors.affiliation: IME Foundation, Department of Laboratory Medicine, PTV-Hospital University Tor Vergata Rome, Rome, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Testi
authors.forename: Sandra
authors.initials: S
authors.lastname: Iannelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Testa
authors.forename: Maria
authors.initials: M
authors.lastname: Troiano
authors.forename: Simonetta
authors.initials: S
authors.lastname: Capelli
authors.forename: Fiorenza
authors.initials: F
authors.lastname: Fruet
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
authors.forename: Andrea
authors.initials: A
authors.lastname: Bontadini
authors.forename: Marco
authors.initials: M
authors.lastname: Andreani
mesh_terms: D059811:HLA-DRB1 Chains / Q000032:analysis*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D008863:Microspheres; D015345:Oligonucleotide Probes / Q000235:genetics; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D059811:HLA-DRB1 Chains; D015345:Oligonucleotide Probes