title: [Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium].
abstract: OBJECTIVE
  To investigate the effects of medicated serum prepared by administration of Jinmaitong (JMT), a compound Chinese herbal medicine, on nicotinamide adenine dinucleotide phosphate (NADPH) oxidase p22-phox subunit and inducible nitric oxide synthase (iNOS) of rat Schwann cells cultured in high-glucose medium.
  
  
  METHODS
  Wistar rats were divided into normal control group (distilled water), JMT (JMT at dose of 1.31 g/(kg·d)) group and vitamin C (vitamin C at dose of 0.08 g/(kg·d)) group to prepare medicated serum. Bilateral sciatic nerves of new born Wistar rats were used to separate Schwann cells. Schwann cells cultured in high-glucose medium were divided into high glucose group (cultured with 50 mmol/L glucose medium), JMT group (cultured with JMT-medicated serum) and vitamin C (VC) group (cultured with VC-medicated serum). Schwann cells cultured in DMEM were used as the normal control. After 48 h culturing, the expression of iNOS was detected by immunofluorescence method and p22-phox mRNA was tested by real-time fluorescent quantitative polymerase chain reaction.
  
  
  RESULTS
  The expression levels of iNOS and p22-phox mRNA in the high glucose group were higher than those in the normal control group (P<0.01). Compared with the high glucose group, expressions of iNOS protein and p22-phox mRNA in JMT group were significantly decreased (P<0.01) and JMT-medicated serum had better effect than VC (P<0.01).
  
  
  CONCLUSIONS
  JMT-medicated serum can down-regulate the expressions of iNOS protein and NADPH oxidase p22-phox subunit mRNA of Schwann cells cultured in high-glucose medium.
authors.affiliation: Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Liang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Huang
authors.forename: Bo-Wu
authors.initials: BW
authors.lastname: Li
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005947:Glucose / Q000009:adverse effects*; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D021122:Protein Subunits / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism*; D044967:Serum
chemical_list: D003470:Culture Media; D004365:Drugs, Chinese Herbal; D021122:Protein Subunits; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D019255:NADPH Oxidases; C515768:Cyba protein, rat; D005947:Glucose