title: Plant redox proteomics.
abstract: In common with other aerobic organisms, plants are exposed to reactive oxygen species resulting in formation of post-translational modifications related to protein oxidoreduction (redox PTMs) that may inflict oxidative protein damage. Accumulating evidence also underscores the importance of redox PTMs in regulating enzymatic activities and controlling biological processes in plants. Notably, proteins controlling the cellular redox state, e.g. thioredoxin and glutaredoxin, appear to play dual roles to maintain oxidative stress resistance and regulate signal transduction pathways via redox PTMs. To get a comprehensive overview of these types of redox-regulated pathways there is therefore an emerging interest to monitor changes in redox PTMs on a proteome scale. Compared to some other PTMs, e.g. protein phosphorylation, redox PTMs have received less attention in plant proteome analysis, possibly due to technical challenges such as with maintaining the in vivo redox states of proteins and the lability of certain PTMs, e.g. nitrosylations, during sample preparation and mass spectrometric analysis. The present review article provides an overview of the recent developments in the emerging area of plant redox proteomics.
authors.affiliation: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Building 224, DK-2800, Kgs Lyngby, Denmark.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Navrot
authors.forename: Christine
authors.initials: C
authors.lastname: Finnie
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
authors.forename: Per
authors.initials: P
authors.lastname: Hägglund
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D054477:Glutaredoxins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
chemical_list: D054477:Glutaredoxins; D010940:Plant Proteins; D020543:Proteome; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013879:Thioredoxins; D014364:Tryptophan; D008715:Methionine; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid