title: The role of CXCR7 on the adhesion, proliferation and angiogenesis of endothelial progenitor cells.
abstract: Previous studies confirmed that stromal cell-derived factor 1 (SDF-1) was a principal regulator of retention, migration and mobilization of haematopoietic stem cells and endothelial progenitor cells (EPCs) during steady-state homeostasis and injury. CXC chemokine receptor 4 (CXCR4) has been considered as the unique receptor of SDF-1 and as the only mediator of SDF-1-induced biological effects for many years. However, recent studies found that SDF-1 could bind to not only CXCR4 but also CXC chemokine receptor 7 (CXCR7). The evidence that SDF-1 binds to the CXCR7 raises a concern how to distinguish the potential contribution of the SDF-1/CXCR7 pathway from SDF-1/CXCR4 pathway in all the processes that were previously attributed to SDF-1/CXCR4. In this study, the role of CXCR7 in EPCs was investigated in vitro. RT-PCR, Western blot and flow cytometry assay demonstrate that both CXCR4 and CXCR7 were expressed highly in EPCs. The adhesion of EPCs induced by SDF-1 was inhibited by blocking either CXCR4 or CXCR7 with their antibodies or antagonists. SDF-1 regulated the migration of EPCs via CXCR4 but not CXCR7. However, the transendothelial migration of EPCs was inhibited by either blocking of CXCR4 or CXCR7. Both CXCR7 and CXCR4 are essential for the tube formation of EPCs induced by SDF-1. These results suggested that both CXCR7 and CXCR4 are important for EPCs in response to SDF-1, indicating that CXCR7 may be another potential target molecule for angiogenesis-dependent diseases.
authors.affiliation: Key laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Dai
authors.forename: Yi
authors.initials: Y
authors.lastname: Tan
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: Lingqiao
authors.initials: L
authors.lastname: Wang
authors.forename: Qunfang
authors.initials: Q
authors.lastname: Ye
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Yan
authors.forename: Kaiwang
authors.initials: K
authors.lastname: Ma
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D000906:Antibodies; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D054387:Receptors, CXCR / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D057705:Transendothelial and Transepithelial Migration / Q000187:drug effects
chemical_list: C501898:Ackr3 protein, rat; D000906:Antibodies; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D054387:Receptors, CXCR; D019718:Receptors, CXCR4