title: Motilibacter peucedani gen. nov., sp. nov., isolated from rhizosphere soil.
abstract: A novel actinomycete strain, designated RP-AC37(T), was isolated from rhizosphere soil collected on Mara Island of Jeju, Republic of Korea. Cells were aerobic, gram-positive, oxidase-negative, catalase-positive, non-mycelium-forming and motile rods (0.6-0.7×1.9-2.4 µm). Phylogenetic analysis based on 16S rRNA gene sequences showed that the organism formed a distinct clade within the radiation occupied by the suborder Frankineae. 16S rRNA gene similarity values were less than 93.2 % to members of the suborder Frankineae and related taxa. The diamino acid isomer in the cell-wall peptidoglycan was LL-diaminopimelic acid. The major whole-cell sugars were glucose, galactose and xylose. The major menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The cellular fatty acids were straight-chain, unsaturated and saturated, with a significant amount of tuberculostearic acid (10-methyl-C(18 : 0)). The DNA G+C content was 73.2 mol%. The combination of morphological, chemotaxonomic and phylogenetic data clearly separate the isolate from members of known genera of the suborder Frankineae and related taxa, suggesting that the isolate represents a novel species in a new genus in this suborder, for which the name Motilibacter peucedani gen. nov., sp. nov. is proposed; the type strain is RP-AC37(T) ( = KCTC 19630(T) = DSM 45328(T)).
authors.affiliation: Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Soon Dong
authors.initials: SD
authors.lastname: Lee
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D019661:Apiaceae / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid