title: Transcriptome analysis reveals dialogues between human trophectoderm and endometrial cells during the implantation period.
abstract: BACKGROUND
  Crosstalk between human trophectoderm (TE) and endometrial cells during the implantation window is a complex and not well-understood process. The aims of this study were (i) to evaluate the global gene expression profile in TE cells from Day 5 human blastocysts issued from IVF, (ii) to compare these data with the transcriptomic profile of endometrial cells in stimulated cycles for IVF and (iii) to identify potential early dialogues between maternal and embryonic cells during the implantation window.
  
  
  METHODS
  Endometrial biopsies (n = 18) from normal responder patients were performed on the day of embryo transfer (Day 5 after human chorionic gonadotrophin administration). TE biopsies from five blastocysts donated for research purposes were mechanically extracted. DNA microarray analysis was carried out to identify the specific gene expression profiles and the biological pathways activated during the implantation window in endometrial and TE cells.
  
  
  RESULTS
  Several cytokines (such as PDGFA, placenta growth factor, IGF2BP1 and IGF2BP3) were up-regulated in human TE cells, whereas some of the corresponding receptors (PDGFRA and KDR) were over-expressed in the receptive endometrium, suggesting that these molecules are involved in the early dialogue between blastocyst and maternal endometrial cells. In addition, several adhesion molecules and extracellular matrix proteins (MCAM, ITGAE and LAMA1) were also over-expressed in the TE, while others (ALCAM, CEACAM1, PECAM1, ITGB8 and LAMA2) were restricted to the receptive endometrium.
  
  
  CONCLUSIONS
  The present study shows that several growth factors, cytokines, integrins and adhesion molecules are expressed in the TE and endometrium at the time of implantation. These results could contribute to the understanding of the mechanisms involved in the early dialogue between blastocyst and endometrium during implantation. Such results should be confirmed by further studies.
authors.affiliation: CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, Montpellier F-34295, France.
authors.forename: D
authors.initials: D
authors.lastname: Haouzi
authors.forename: H
authors.initials: H
authors.lastname: Dechaud
authors.forename: S
authors.initials: S
authors.lastname: Assou
authors.forename: C
authors.initials: C
authors.lastname: Monzo
authors.forename: J
authors.initials: J
authors.lastname: de Vos
authors.forename: S
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D010064:Embryo Implantation / Q000235:genetics*; D004624:Embryo Transfer; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D006801:Humans; D011247:Pregnancy; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis; D015398:Signal Transduction; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D020796:Receptor, Platelet-Derived Growth Factor alpha