title: Purification of immunoglobulins using protein a-sepharose.
abstract: Most immunochemical techniques used in molecular biology rely on a specific class of antibodies, immunoglobulin G (IgG). In mammals, IgG antibodies are produced during the secondary humoral response and contribute about 80% of the serum immunoglobulin. Factors affecting the experimental production of antibodies have been discussed in detail (1). In this chapter we describe a method for the rapid and efficient purification of IgG to near homogeneity from rabbit serum. This is achieved by affinity chromatography using protein A.
authors.affiliation: Biochemistry Department, Rothamsted Experimental Station, Hertfordshire, UK.
authors.forename: N J
authors.initials: NJ
authors.lastname: Kruger
authors.forename: J B
authors.initials: JB
authors.lastname: Hammond