title: Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism.
abstract: The antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) is a strong bactericide when unpolymerized and has the potential to be utilized in various resinous biomaterials. To analyze the antibacterial characteristics of this monomer in detail, the ability of high concentrations of unpolymerized MDPB to kill Streptococcus mutans in planktonic or biofilm forms within a short time-period of contact, and the inhibitory effects of low concentrations of MDPB on the metabolic function of S. mutans, were examined. High concentrations of MDPB showed effective killing of planktonic and biofilm S. mutans cells within 60 s, and complete killing was obtained by contact with 1,000 μg ml(-1) of MDPB for 60 s. At a concentration of 4-8 μg ml(-1) , MDPB demonstrated growth inhibition, inducing elongation of the lag phase and of the doubling time, when the bacterial number was low. Inhibition of the production of acid from S. mutans by 8 μg ml(-1) of MDPB may have been caused by the inhibition of lactate dehydrogenase activity. At high concentrations, MDPB is lethal to both planktonic and biofilm forms of S. mutans in a short time-period, and at low concentrations, MDPB inhibits metabolic enzymatic activity.
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita, UK.
authors.forename: Naomi
authors.initials: N
authors.lastname: Izutani
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nakajo
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takahashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
authors.forename: Roy R B
authors.initials: RR
authors.lastname: Russell
mesh_terms: D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D011726:Pyridinium Compounds / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D011726:Pyridinium Compounds; C089392:methacryloyloxydodecylpyridinium bromide