title: Two-photon excitation of 2,5-diphenyloxazole using a low power green solid state laser.
abstract: This Letter concerns two-photon excitation of 2,5-Diphenyloxazole (PPO) upon illumination from a pulsed 532 nm solid state laser, with an average power of 30 mW, and a repetition rate of 20 MHz. A very agreeable emission spectrum position and shape has been achieved for PPO receiving one- and two-photon excitation, which suggests that the same excited state is involved for both excitation modes. Also, a perfect quadratic dependence of laser power in the emission intensity function has been recorded. We tested the application of a small solid state green laser to two-photon induced time-resolved fluorescence, revealing the emission anisotropy of PPO to be considerably higher for two-photon than for one-photon excitation.
authors.affiliation: Center for Commercialization of Fluorescence Technologies (CCFT), Department of Molecular Biology and Immunology, UNTHSC, Fort Worth, TX 76107, USA.
authors.forename: Rafal
authors.initials: R
authors.lastname: Luchowski