title: Institutional Profile: The Sheffield RNAi screening facility: a service for high-throughput, genome-wide Drosophila RNAi screens.
abstract: The Sheffield RNAi Screening Facility (SRSF) was established in November 2008, as Britain's first Drosophila RNAi screening centre, funded by the University of Sheffield, Biomedical Sciences Department and the Wellcome Trust. The SRSF was formed to service the needs of research groups wanting to carry out high-throughput RNAi screens with Drosophila cells. The rationale for the SRSF is to provide RNAi libraries and the specialist equipment and expertise to do such screens. The facility supports both plate reader assays, high-content microscopy as well as the equipment needed to process these samples in a high-throughput fashion. The SRSF can either be used to identify genes involved in disease representing future drug targets, or to identify genes involved in drug resistance and efficacy.
authors.affiliation: The Sheffield RNAi Screening Facility, Alfred Denny Building, University of Sheffield, Western Bank, Sheffield, Yorkshire, S10 2TN, UK. rnai@sheffield.ac.uk
authors.forename: Stephen
authors.initials: S
authors.lastname: Brown
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D049750:Genome, Insect; D023281:Genomics / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D034622:RNA Interference; D006113:United Kingdom; D014495:Universities