title: Generic protease detection technology for monitoring periodontal disease.
abstract: Periodontal diseases are inflammatory conditions that affect the supporting tissues of teeth and can lead to destruction of the bone support and ultimately tooth loss if untreated. Progression of periodontitis is usually site specific but not uniform, and currently there are no accurate clinical methods for distinguishing sites where there is active disease progression from sites that are quiescent. Consequently, unnecessary and costly treatment of periodontal sites that are not progressing may occur. Three proteases have been identified as suitable markers for distinguishing sites with active disease progression and quiescent sites: human neutrophil elastase, cathepsin G and MMP8. Generic sensor materials for the detection of these three proteases have been developed based on thin dextran hydrogel films cross-linked with peptides. Degradation of the hydrogel films was monitored using impedance measurements. The target proteases were detected in the clinically relevant range within a time frame of 3 min. Good specificity for different proteases was achieved by choosing appropriate peptide cross-linkers.
authors.affiliation: School of Engineering and Materials Science, Queen Mary University of London, London, El 4NS, UK.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Zheng
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Cook
authors.forename: Michael
authors.initials: M
authors.lastname: Watkinson
authors.forename: Shoufeng
authors.initials: S
authors.lastname: Yang
authors.forename: Ian
authors.initials: I
authors.lastname: Douglas
authors.forename: Andrew
authors.initials: A
authors.lastname: Rawlinson
authors.forename: Steffi
authors.initials: S
authors.lastname: Krause
mesh_terms: D015415:Biomarkers / Q000032:analysis; D017097:Electric Impedance; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D010447:Peptide Hydrolases / Q000032:analysis*; D010510:Periodontal Diseases / Q000175:diagnosis / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D020100:Hydrogels; D010447:Peptide Hydrolases