title: Trypanosoma evansi: Activities of adenine nucleotide degradation enzymes in cerebral cortex of infected rats.
abstract: This study aimed to evaluate the activities of the ectoenzymes NTPDase and 5'-nucleotidase in synaptosomes from cerebral cortex of rats experimentally infected with Trypanosoma evansi. The animals were divided in four groups (n=10) according to the time and degree of parasitemia (groups A, B, C and D). The animals from group A were euthanized on day 3 (low parasitemia), group B on day 5 (high parasitemia) and group C on day 15 (low parasitemia). Group D consisted of healthy rats (not-infected, n=15) and were divided in three periods (n=5) in order to compare with the infected groups. After euthanasia, cerebral cortex was removed for the preparation of synaptosomes and enzymatic assays. Group A showed no changes in enzymatic activities compared with control. The hydrolysis of ATP, ADP and AMP by the enzymes NTPDase and 5'-nucleotidase were increased (P<0.05) in group B (38%, 140% and 61%, respectively) when compared with control. In the group C it was observed a decreased (22%) hydrolysis of ATP when compared with control group. The activities of NTPDase and 5'-nucleotidase in synaptosomes alters the acute phase of the disease when the number of circulating parasites is high, thus the change observed is probably due to the parasitemia.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil. camilabelmontevet@yahoo.com.br
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.forename: Rosélia
authors.initials: R
authors.lastname: Spanevello
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Viviane C G
authors.initials: VC
authors.lastname: Souza
authors.forename: Victor C
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Gustavo R
authors.initials: GR
authors.lastname: Thomé
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Sonia T A
authors.initials: ST
authors.lastname: Lopes
authors.forename: Daniela B R
authors.initials: DB
authors.lastname: Leal
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology* / Q000473:pathology; D004285:Dogs; D008297:Male; D018512:Parasitemia / Q000201:enzymology / Q000469:parasitology; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000201:enzymology; D014352:Trypanosomiasis / Q000201:enzymology* / Q000469:parasitology / Q000473:pathology
chemical_list: D000227:Adenine Nucleotides; D015720:5'-Nucleotidase; D011755:Pyrophosphatases