title: Assembling a dual purpose TaqMan-based panel of single-nucleotide polymorphism markers in rainbow trout and steelhead (Oncorhynchus mykiss) for association mapping and population genetics analysis.
abstract: We establish a TaqMan-based assay panel for genotyping single-nucleotide polymorphisms in rainbow trout and steelhead (Oncorhynchus mykiss). We develop 22 novel single-nucleotide polymorphism markers based on new steelhead sequence data and on assays from sister taxa. Additionally, we adapt 154 previously developed markers to the TaqMan platform. At the beginning of this study, 59 SNPs with TaqMan assays were available to the scientific community. By adding 176 additional TaqMan assays to this number, we greatly expand the biological applications of TaqMan genotyping within both population genetics and quantitative genetics.
authors.affiliation: Institute of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Denmark. MetteH.Hansen@agrsci.dk
authors.forename: Mette H H
authors.initials: MH
authors.lastname: Hansen
authors.forename: Sewall
authors.initials: S
authors.lastname: Young
authors.forename: Hanne B H
authors.initials: HB
authors.lastname: Jørgensen
authors.forename: Carita
authors.initials: C
authors.lastname: Pascal
authors.forename: Mark
authors.initials: M
authors.lastname: Henryon
authors.forename: James
authors.initials: J
authors.lastname: Seeb
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003718:Denmark; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D014861:Washington
chemical_list: D005819:Genetic Markers