title: Two-dimensional strong cation-exchange liquid chromatography/reversed-phase pressurized capillary electrochromatography for separation of complex samples.
abstract: A 2-D separation platform was constructed using micro strong cation-exchange liquid chromatography (μ-SCXLC) and reversed-phase pressurized capillary electrochromatography (RP-pCEC) for the analysis of complex samples. Samples were fractionated by the first-dimension μ-SCXLC with a linear solvent gradient and then injected into the second-dimension RP-pCEC for further separation. The μ-SCXLC/RP-pCEC 2-D system with three separation mechanisms, namely strong cation-exchange, reversed-phase chromatography and electrophoresis, provided high selectivity, high resolution and high peak capacity compared to one-dimensional chromatographic approaches. Separation effectiveness of this 2-D system was demonstrated by the analysis of different kinds of complex samples, such as traditional Chinese medicine Cortex Phellodendri, bovine serum albumin (BSA) tryptic digest and real serum tryptic digest. A theoretical peak capacity of approximately 1200 was achieved, which proves its promising potential for the separation and analysis of complex samples.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P R China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Gu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D000818:Animals; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002411:Cation Exchange Resins / Q000737:chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D002411:Cation Exchange Resins; D004365:Drugs, Chinese Herbal; D012710:Serum Albumin, Bovine