title: Dynamic intracellular reorganization of cytoskeletons and the vacuole in defense responses and hypersensitive cell death in plants.
abstract: Plants have evolved various means for controlled and organized cell destruction, known as programmed cell death (PCD). In plant immune responses against microbial infection, hypersensitive cell death as a form of PCD is a crucial event to prevent the spread of biotrophic pathogens. Recent live cell imaging techniques have revealed dynamic features and significant roles of cytoskeletons and the vacuole during defense responses and the PCD. Actin microfilaments (MFs) focus on the infection sites and function as tracks for the polar transport of antimicrobial materials. To accomplish hypersensitive cell death, further dynamic changes in cytoskeletons are induced. MFs play a role in the structural and functional regulation of the vacuole, leading to execution of the PCD. We here overview spatiotemporal dynamic changes in the cytoskeletons and the vacuoles triggered by signals from pathogens, and propose a hypothetical model for MF-regulated vacuole-mediated PCD in plant immunity.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Higaki
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Kurusu
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Hasezawa
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Kuchitsu
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology*; D000199:Actins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D016923:Cell Death; D002473:Cell Wall / Q000502:physiology; D003593:Cytoplasm; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D007425:Intracellular Membranes / Q000276:immunology* / Q000502:physiology; D059828:Plant Cells; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D000199:Actins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002118:Calcium