title: Development of imaging mass spectrometry (IMS) dataset extractor software, IMS convolution.
abstract: Imaging mass spectrometry (IMS) is a powerful tool for detecting and visualizing biomolecules in tissue sections. The technology has been applied to several fields, and many researchers have started to apply it to pathological samples. However, it is very difficult for inexperienced users to extract meaningful signals from enormous IMS datasets, and the procedure is time-consuming. We have developed software, called IMS Convolution with regions of interest (ROI), to automatically extract meaningful signals from IMS datasets. The processing is based on the detection of common peaks within the ordered area in the IMS dataset. In this study, the IMS dataset from a mouse eyeball section was acquired by a mass microscope that we recently developed, and the peaks extracted by manual and automatic procedures were compared. The manual procedure extracted 16 peaks with higher intensity in mass spectra averaged in whole measurement points. On the other hand, the automatic procedure using IMS Convolution easily and equally extracted peaks without any effort. Moreover, the use of ROIs with IMS Convolution enabled us to extract the peak on each ROI area, and all of the 16 ion images on mouse eyeball tissue were from phosphatidylcholine species. Therefore, we believe that IMS Convolution with ROIs could automatically extract the meaningful peaks from large-volume IMS datasets for inexperienced users as well as for researchers who have performed the analysis.
authors.affiliation: Department of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.forename: Masaru
authors.initials: M
authors.lastname: Ushijima
authors.forename: Ikuko
authors.initials: I
authors.lastname: Yao
authors.forename: Akiko
authors.initials: A
authors.lastname: Yuba-Kubo
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Wakui
authors.forename: Shigeki
authors.initials: S
authors.lastname: Kajihara
authors.forename: Masaaki
authors.initials: M
authors.lastname: Matsuura
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007477:Ions / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010713:Phosphatidylcholines / Q000032:analysis*; D012160:Retina / Q000737:chemistry / Q000648:ultrastructure*; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D007477:Ions; D010713:Phosphatidylcholines