title: Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli.
abstract: Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artificial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-specific metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artificial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor, caffeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, 685-1 Yangcheong-ri Ochang-eup, Chungbuk 363-883, Republic of Korea.
authors.forename: Oksik
authors.initials: O
authors.lastname: Choi
authors.forename: Cheng-Zhu
authors.initials: CZ
authors.lastname: Wu
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kang
authors.forename: Jong Seog
authors.initials: JS
authors.lastname: Ahn
authors.forename: Tai-Boong
authors.initials: TB
authors.lastname: Uhm
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Hong
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology; D053898:Biosynthetic Pathways; D003066:Coenzyme A Ligases / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D044950:Flavanones / Q000096:biosynthesis; D005419:Flavonoids / Q000096:biosynthesis / Q000378:metabolism; D005818:Genetic Engineering; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; D044950:Flavanones; D005419:Flavonoids; D013267:Stilbenes; C004999:ferulic acid; D008780:Methyltransferases; C019608:caffeate O-methyltransferase; D000217:Acyltransferases; C022674:stilbene synthase; C020141:flavanone synthetase; D003066:Coenzyme A Ligases; C005273:naringenin; D000077185:Resveratrol