title: Identification of a novel apoptosis suppressor gene from the baculovirus Lymantria dispar multicapsid nucleopolyhedrovirus.
abstract: Ld652Y cells from Lymantria dispar readily undergo apoptosis upon infection with a variety of nucleopolyhedroviruses (NPVs), while L. dispar multicapsid NPV (LdMNPV) infection of Ld652Y cells results in the production of a high titer of progeny viruses. Here, we identify a novel LdMNPV apoptosis suppressor gene, apsup, which functions to suppress apoptosis induced in Ld652Y cells by infection with vAcΔp35, a p35-defective recombinant Autographa californica MNPV. apsup also suppresses apoptosis of Ld652Y cells induced by actinomycin D and UV exposure. Apsup is expressed in LdMNPV-infected Ld652Y cells late in infection, and RNA interference-mediated apsup ablation induces apoptosis of LdMNPV-infected Ld652Y cells.
authors.affiliation: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Yamada
authors.forename: Miyuki
authors.initials: M
authors.lastname: Shibuya
authors.forename: Michihiro
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Motoko
authors.initials: M
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D009036:Moths / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D051017:Apoptosis Regulatory Proteins