title: Gold nanoparticles deposited capillaries for in-capillary microextraction capillary zone electrophoresis of monohydroxy-polycyclic aromatic hydrocarbons.
abstract: This article presents the first application of gold nanoparticles deposited capillaries as pre-concentration devices for in-capillary microextraction CZE and their use for the analysis of monohydroxy-polycyclic aromatic hydrocarbons in synthetic urine samples. The successful separation of 1-hydroxypyrene, 9-hydroxyphenanthrene, 3-hydroxybenzo[a]pyrene (3-OHbap), 4-hydroxybenzo[a]pyrene and 5-hydroxybenzo[a]pyrene under a single set of electrophoretic conditions is demonstrated as well as the feasibility to obtain competitive ultraviolet absorption LOD with commercial instrumentation. Enrichment factors ranging from 87 (9-OHphe) to 100 (3-OHbap) made it possible to obtain LOD ranging from 9 ng/mL (9-OHphe and 3-OHbap) to 14 ng/mL (4-hydroxybenzo[a]pyrene).
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, FL 32816-2366, USA.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Wang
authors.forename: Gaston
authors.initials: G
authors.lastname: Knobel
authors.forename: Walter B
authors.initials: WB
authors.lastname: Wilson
authors.forename: Korina
authors.initials: K
authors.lastname: Calimag-Williams
authors.forename: Andres D
authors.initials: AD
authors.lastname: Campiglia
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D006046:Gold