title: Sporosarcina newyorkensis sp. nov. from clinical specimens and raw cow's milk.
abstract: Twelve independent isolates of a gram-positive, endospore-forming rod were recovered from clinical specimens in New York State, USA, and from raw milk in Flanders, Belgium. The 16S rRNA gene sequences for all isolates were identical. The closest species with a validly published name, based on 16S rRNA gene sequence, is Sporosarcina koreensis (97.13 % similarity). DNA-DNA hybridization studies demonstrate that the new isolates belong to a species distinct from their nearest phylogenetic neighbours. The partial sequences of the 23S rRNA gene for the novel strains and their nearest neighbours also provide support for the novel species designation. Maximum-likelihood phylogenetic analysis of the 16S rRNA gene sequences confirmed that the new isolates are in the genus Sporosarcina. The predominant menaquinone is MK-7, the peptidoglycan has the type A4α L-Lys-Gly-D-Glu, and the polar lipids consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant fatty acids are iso-C(14 : 0), iso-C(15 : 0) and anteiso-C(15 : 0). In addition, biochemical and morphological analyses support designation of the twelve isolates as representatives of a single new species within the genus Sporosarcina, for which the name Sporosarcina newyorkensis sp. nov. (type strain 6062(T)  = DSM 23544(T)  = CCUG 59649(T)  = LMG 26022(T)) is proposed.
authors.affiliation: Department of Biomedical Sciences, State University of New York, Albany, NY 12201, USA.|Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Wolfgang
authors.affiliation: Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.|Department of Applied Engineering Sciences, Laboratory of Biochemistry and Brewing, University College Ghent, Schoonmeersstraat 52, 9000 Ghent, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Coorevits
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: Jocelyn A
authors.initials: JA
authors.lastname: Cole
authors.affiliation: Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: Michelle C
authors.initials: MC
authors.lastname: Dickinson
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: George E
authors.initials: GE
authors.lastname: Hannett
authors.affiliation: Department of Biomedical Technology, Albany College of Pharmacy, 106 New Scotland Ave, Albany, NY 12208, USA.
authors.forename: Reashma
authors.initials: R
authors.lastname: Jose
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Nazarian
authors.affiliation: DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.|Department of Applied Engineering Sciences, Laboratory of Biochemistry and Brewing, University College Ghent, Schoonmeersstraat 52, 9000 Ghent, Belgium.
authors.forename: Anita
authors.initials: A
authors.lastname: Van Landschoot
authors.affiliation: Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: Samantha E
authors.initials: SE
authors.lastname: Wirth
authors.affiliation: Department of Biomedical Sciences, State University of New York, Albany, NY 12201, USA.|Wadsworth Center, Bacteriology Laboratory, New York State Department of Health, PO Box 22002, Albany, NY 12201, USA.
authors.forename: Kimberlee A
authors.initials: KA
authors.lastname: Musser
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D000818:Animals; D015373:Bacterial Typing Techniques; D001530:Belgium / Q000453:epidemiology; D002417:Cattle; D005260:Female; D020459:Genes, rRNA; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009518:New York / Q000453:epidemiology; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D058644:Sporosarcina / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S