title: Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage.
abstract: Micro-RNAs (miRNAs) have been recognized as critical regulators of gene expression, and deregulation of miRNA expression has been implicated in a wide spectrum of diseases. To provide a framework for the role of miRNAs in B-cell development and malignancy, we deep-sequenced miRNAs from B1 cells and 10 developmental stages that can be identified within the mouse B2 B-cell lineage. The expression profiles of the 232 known miRNAs that are expressed during B-cell development display stage-specific induction patterns, yet hierarchical clustering analysis showed relationships that are in full agreement with the model of the B2 B-cell developmental pathway. Analysis of exemplary miRNA expression profiles (miR-150, miR-146a, miR-155, miR-181) confirmed that our data are in agreement with previous results. The high resolution of the expression data allowed for the identification of the sequential expression of oncomir-1/miR-17-92 and its paralogs miR-106a-363 and miR-106b-25 in subsequent developmental stages in the BM. Further, we have identified and validated 45 novel miRNAs and 6 novel miRNA candidates expressed in developing B cells.
authors.affiliation: Department of Immunology, Hartwell Center for Bioinformatics and Biotechnology, St Jude Children’s Research Hospital, Memphis, TN 38105, USA.
authors.forename: Diana C
authors.initials: DC
authors.lastname: Spierings
authors.forename: Daniel
authors.initials: D
authors.lastname: McGoldrick
authors.forename: Ann Marie
authors.initials: AM
authors.lastname: Hamilton-Easton
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Neale
authors.forename: Elizabeth P
authors.initials: EP
authors.lastname: Murchison
authors.forename: Greg J
authors.initials: GJ
authors.lastname: Hannon
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Green
authors.forename: Sebo
authors.initials: S
authors.lastname: Withoff
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000145:classification / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D022661:RNA, Untranslated