title: Detection of species-specific long VNTRs in mitochondrial control region and their application to identifying sympatric Hong Kong grouper (Epinephelus akaara) and yellow grouper (Epinephelus awoara).
abstract: Length variation and heteroplasmy were observed in PCR products of the first half of mtDNA control region of both Hong Kong grouper (Epinephelus akaara) and yellow grouper (Epinephelus awoara). DNA sequencing unveiled the phenomena were caused by the presence of species-specific long variable number tandem repeats (VNTRs). This is the first report on the mtDNA VNTRs and their heteroplasmy in groupers. Moreover, these VNTRs are also the longest such structure found in teleost fish. Thereafter, we designed two species-specific PCR reverse primers according to the 3' end sequences of the VNTRs and successfully established assays for the identification of these two sympatric grouper species.
authors.affiliation: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, No. 19 XinJieKouWai Avenue, Beijing 100875, China. jiehan@bnu.edu.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.forename: Fenghua
authors.initials: F
authors.lastname: Lv
authors.forename: Houcai
authors.initials: H
authors.lastname: Cai
mesh_terms: D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D006723:Hong Kong; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial