title: Platelet-derived growth factor receptor alpha is associated with oxidative stress-induced retinal cell death.
abstract: OBJECTIVE
  The purpose of this study was to investigate the role played by platelet-derived growth factor-α (PDGFRα) in oxidative stress-induced retinal cell death. A previous proteomic study from our laboratory showed that expression of PDGFRα is elevated considerably in the retinas of an animal model of glaucoma-the excitatory amino acid carrier (EAAC) 1-deficient (EAAC1-/-) mouse.
  
  
  METHODS
  Retinal sites and expression patterns of PDGFRα were determined by immunohistochemistry in the retinas of EAAC1-/- and control CRL:CD1(ICR) mice. A retinal cell line was exposed to hydrogen peroxide, and expression PDGFRα determined by Western blot analysis. Effects of PDGF-AA and PDGFRα-siRNA on hydrogen peroxide-induced retinal cell death were examined.
  
  
  RESULTS
  PDGFRα was detected in the retinal ganglion cell layer (RGL) of both EAAC1-/- and ICR mice, and was also localized in the internal nuclear layer (INL) of EAAC1-/- mice. While treatment with excess PDGF-AA had no additional effect on retinal cell death, expression of PDGFRα increased with exposure to hydrogen peroxide. Hydrogen peroxide-induced retinal cell death was inhibited by exposure to PDGF-AA via phosphatidylinositol 3 kinase (PI3K); cell death was promoted by PDGFRα-siRNA.
  
  
  CONCLUSIONS
  PDGFRα is expressed in mouse retina, where it is essential for retinal cell survival under conditions of oxidative stress.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. tkana@hiroshima-u.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamoto
authors.forename: Ulfah
authors.initials: U
authors.lastname: Rimayanti
authors.forename: Okumichi
authors.initials: O
authors.lastname: H
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kiuchi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D050580:Excitatory Amino Acid Transporter 3 / Q000235:genetics; D025461:Feedback, Physiological; D019084:Fluorescent Antibody Technique, Indirect; D006861:Hydrogen Peroxide / Q000633:toxicity; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000494:pharmacology; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000502:physiology*; D012160:Retina / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*
chemical_list: D050580:Excitatory Amino Acid Transporter 3; D010982:Platelet-Derived Growth Factor; D034741:RNA, Small Interfering; C493399:Slc1a1 protein, mouse; C075278:platelet-derived growth factor A; D006861:Hydrogen Peroxide; D020796:Receptor, Platelet-Derived Growth Factor alpha