title: Small-molecule fluorescent probes for specific RNA targets.
abstract: A method was developed that uses small molecules as fluorescent probes to detect specific mRNAs. In this approach, the fluorescence of fluorophore-quencher conjugates is restored by the binding of an mRNA aptamer tag to the quencher segment of the molecules. The method allows real-time detection of mRNA transcripts in vitro.
authors.affiliation: Institute for Integrated Cell-Material Sciences, Kyoto University Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Murata
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sato
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawazoe
authors.forename: Motonari
authors.initials: M
authors.lastname: Uesugi
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012333:RNA, Messenger