title: Characterization of the TAK1 gene in Apis cerana cerana (AccTAK1) and its involvement in the regulation of tissue-specific development.
abstract: TGF-Β activated kinase-1 (TAK1) plays a pivotal role in developmental processes in many species. Previous research has mainly focused on the function of TAK1 in model organisms, and little is known about the function of TAK1 in hymenoptera insects. Here, we isolated and characterized the TAK1 gene from Apis cerana cerana. Promoter analysis of AccTAK1 revealed the presence of transcription factor binding sites related to early development. Real-time quantitative PCR and immunohistochemistry experiments revealed that AccTAK1 was expressed at high levels in fourth instar larvae, primarily in the abdomen, in the intestinal wall cells of the midgut and in the secretory cells of the salivary glands. In addition, AccTAK1 expression in fourth instar larvae could be dramatically induced by treatment with pesticides and organic solvents. These observations suggest that AccTAK1 may be involved in the regulation of early development in the larval salivary gland and midgut.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Taian, Shandong, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Meng
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Kang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Luo
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology; D020930:MAP Kinase Kinase Kinases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010575:Pesticides / Q000494:pharmacology; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D012997:Solvents / Q000494:pharmacology; D014018:Tissue Distribution; D000099151:MAP Kinase Kinase Kinase 7
chemical_list: D020930:MAP Kinase Kinase Kinases; D010575:Pesticides; D012997:Solvents; D000099151:MAP Kinase Kinase Kinase 7