title: [Correlation study of the overexpression of activated Cdc42-associated kinase 1 and the stage and prognosis of esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression and relationship of activated Cdc42-associated kinase 1 (ACK1) and the clinical characteristics of esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  The ACK1 expression in ESCC cell lines was detected by Western blot. Immunohistochemistry was performed to assay the expression level of ACK1 protein in tumor tissues and adjacent normal epithelium from 105 ESCC patients in tissue microarray and 45 patients in normal tissue slices. Semi-quantitative RT-PCR(reverse transcription-polymerase chain reaction)was performed to determine the expression level of ACK1 mRNA in 45 pairs of ESCC frozen tissues.
  
  
  RESULTS
  The expression level of ACK1 protein was significantly up-regulated in 48.6% ESCC tissues as compared with the normal adjacent epithelium in tissue microarray. The overexpression of ACK1 was significantly correlated with the lymph node metastasis and TNM stage of ESCC patients. The results of normal tissue slices were consistent with those of tissue microarray. Furthermore the overexpression of ACK1 was associated with a poor survival of ESCC patients (P = 0.030). The elevated mRNA level of ACK1 in ESCC tissues was correlated with the lymph node metastasis and TNM stage of ESCC patients. And a significant correlation was observed between protein and mRNA level of ACK1 (P = 0.021).
  
  
  CONCLUSIONS
  The up-regulated expressions of ACK1 protein and mRNA are correlated with the progression and prognosis of ESCC.
authors.affiliation: Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Zhao-li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Chang
authors.forename: Bao-zhong
authors.initials: BZ
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Tan
authors.forename: Su-sheng
authors.initials: SS
authors.lastname: Shi
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: He
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: D011505:Protein-Tyrosine Kinases; C403262:TNK2 protein, human