title: Catabolite repression of the Bacillus subtilis FadR regulon, which is involved in fatty acid catabolism.
abstract: The Bacillus subtilis fadR regulon involved in fatty acid degradation comprises five operons, lcfA-fadR-fadB-etfB-etfA, lcfB, fadN-fadA-fadE, fadH-fadG, and fadF-acdA-rpoE. Since the lcfA-fadRB-etfBA, lcfB, and fadNAE operons, whose gene products directly participate in the β-oxidation cycle, had been found to be probably catabolite repressed upon genome-wide transcript analysis, we performed Northern blotting, which indicated that they are clearly under CcpA-dependent catabolite repression. So, we searched for catabolite-responsive elements (cre's) to which the complex of CcpA and P-Ser-HPr binds to exert catabolite repression by means of a web-based cis-element search in the B. subtilis genome using known cre sequences, which revealed the respective candidate cre sequences in the lcfA, lcfB, and fadN genes. DNA footprinting indicated that the complex actually interacted with these cre's in vitro. Deletion analysis of each cre using the lacZ fusions with the respective promoter regions of the three operons with and without it, indicated that these cre's are involved in the CcpA-dependent catabolite repression of the operons in vivo.
authors.affiliation: Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Tojo
authors.forename: Takenori
authors.initials: T
authors.lastname: Satomura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuoka
authors.forename: Kazutake
authors.initials: K
authors.lastname: Hirooka
authors.forename: Yasutaro
authors.initials: Y
authors.lastname: Fujita
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001665:Binding Sites; D015152:Blotting, Northern; D057465:Catabolite Repression; D018983:DNA Footprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C057119:FadR protein, Bacteria; D005227:Fatty Acids; D012097:Repressor Proteins