title: The isolation and characterization of simultaneous saccharification and fermentation microorganisms for Laminaria japonica utilization.
abstract: Brown seaweed contains various carbohydrates, such as alginate, laminaran, and mannitol, therefore ethanol fermentation was attempted with Nuruk and a mixed culture that included Laminaria japonica. Nuruk is used to make Korean traditional alcohol. In the research, four microorganisms that produced ethanol and had the ability to achieve alginate degradation were obtained on the L. japonica medium. Nuruk 4 was found to produce a better result than the other tested microorganisms, and the optimal substrate for ethanol production was found to be mannitol (2.59 g/L at 96 h). Nuruk 4 was more than three times better compared with Candida tropicalis in regards to ethanol production. When alginate lyase activity occurred, it appeared as a clear zone around Nuruk 3. The maximal ethanol production yield conditions were comprised of Nuruk 3 and 4 on the anaerobic culture. In this case, 2.0 g/L of ethanol were efficiently produced under the same conditions.
authors.affiliation: Department of Bioscience and Biotechnology, Silla University, Busan 617-736, Republic of Korea.
authors.forename: Sung-Mok
authors.initials: SM
authors.lastname: Lee
authors.forename: Jae-Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D007795:Laminaria / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism
chemical_list: D003470:Culture Media; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase