title: [Expression of Gluc-Fluc dual luciferase plasmid after transfection into MB49 bladder cells].
abstract: OBJECTIVE
  To investigate the expression characteristics of Gluc-Fluc dual luciferase plasmid after its transfection into MB49 bladder cells.
  
  
  METHODS
  pAAV2neoCAG-Gluc-2A-Fluc and pAAV2neo-Gluc plasmids were separately transfected into MB49 cells via LipofectamineTM2000. The Gluc activity in the cell culture supernatant and the Fluc activity in the cells were detected by luminometer and Lumina Imaging system.
  
  
  RESULTS
  The luminometer result showed that the activity of Gluc in the supernatant increased gradually in a cell number- and time-dependent manner, while Fluc activity in the cells increased with the cell number but not with time. The Lumina Imaging system showed that Gluc-Fluc was successfully expressed in MB49 bladder cells and cell lines with stable Gluc-Fluc expression were obtained after G418 selection.
  
  
  CONCLUSIONS
  Gluc in the dual luciferase plasmid retains its expression characteristics. Due to the advantages of Fluc in localization in living imaging and the easy quantitative detection of Gluc, the dual luciferase plasmid, after transfection in MB49 bladder cells, allows reliable and dynamic detection of tumor growth in animal models.
authors.affiliation: Department of Urinary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. nfzhangxinji@sohu.com
authors.forename: Xin-Ji
authors.initials: XJ
authors.lastname: ZHANG
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: SHI
authors.forename: Peng-yu
authors.initials: PY
authors.lastname: SUN
authors.forename: Zhe-huan
authors.initials: ZH
authors.lastname: ZHANG
authors.forename: Xin-yang
authors.initials: XY
authors.lastname: FU
authors.forename: Wan-long
authors.initials: WL
authors.lastname: TAN
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D008156:Luciferases