title: [Analysis the advantage of autologous mobilized peripheral blood mononuclear cells transplantation on lower limbs ischemia disease].
abstract: OBJECTIVE
  To analyze the efficacy and its correlation with species of transplant cells of autologous mobilized peripheral blood (PB) mononucleated cells (MNCs) transplantation on 59 patients with lower limbs ischemia.
  
  
  METHODS
  Fifty-nine patients were evaluated with symptoms scores and after that their PBMNCs were mobilized and collected and then injected into the ischemic area at equal distance. They effectiveness and scores were evaluated at 7th day and 4th month after therapy. The correlation of CD34(+) cells and of MNCs with effectiveness was analysed respectively, and formula for correlations between them and effectiveness was calculated.
  
  
  RESULTS
  After MNCs injection, the effectiveness was observed both at 7th day and 4th month. The correlation of MNCs with effectiveness was stronger than that of CD34(+) cells (the effectiveness was represented by nimodipine value), According to the formula of nimodipine value, the value of the latter = 0.484 + 1.055 × CD34(+) cells number and the former = 0.288 + 0.401 × MNCs number with a correlation coefficient of R = 0.461 (P = 0.047) and R = 0.473 (P = 0.000) respectively.
  
  
  CONCLUSIONS
  Autologous mobilized PBMNCs number is a better indicator for effectiveness than CD34(+) cells number.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ping-ping
authors.initials: PP
authors.lastname: Huang
authors.forename: Shang-zhu
authors.initials: SZ
authors.lastname: Li
authors.forename: Ying-xin
authors.initials: YX
authors.lastname: Zhao
authors.forename: Feng-lei
authors.initials: FL
authors.lastname: Long
authors.forename: Ren-min
authors.initials: RM
authors.lastname: Fu
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Zheng
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Liu
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Liu
authors.forename: Jun-fan
authors.initials: JF
authors.lastname: Li
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Hu
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
mesh_terms: D005260:Female; D006801:Humans; D007511:Ischemia / Q000601:surgery*; D035002:Lower Extremity / Q000098:blood supply; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000637:transplantation*; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D016491:Peripheral Vascular Diseases / Q000601:surgery*; D014182:Transplantation, Autologous