title: [siRNA-mediated inhibition of ErbB2 expression and its effect on breast cancer cell growth].
abstract: OBJECTIVE
  To construct a vector of ErbB2 small interfering RNA(siRNA), and to investigate its effect on ErbB2 expression and the growth of ZR75-1 breast cancer cells.
  
  
  METHODS
  Two ErbB2-siRNAs were designed and inserted into the pSliencer 2.1-U6 neo vector. After confirmed by restriction and DNA sequencing, siRNA vectors were co-transfected with the FLAG-ErbB2 expression vector into human embryonic kidney 293T cells, or transfected into SKBR3 and ZR75-1 breast cancer cells. The effects of siRNAs on the expression of ErbB2 were identified by Western blot and the proliferation of ZR75-1 cells was repressed.
  
  
  RESULTS
  Two siRNAs could effectively inhibit the expression of exogenous and endogenous ErbB2 proteins, and could repress the growth of ZR75-1 cells.
  
  
  CONCLUSIONS
  ErbB2 siRNAs effectively inhibit the expression of ErbB2, thus repressing the growth of ZR75-1 cells.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100850, China. jiangkai@ymail.com
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Yang
authors.forename: Zhao-yun
authors.initials: ZY
authors.lastname: Wang
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Xu
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Han
authors.forename: Qi-nong
authors.initials: QN
authors.lastname: Ye
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D018014:Gene Transfer Techniques / Q000295:instrumentation; D005822:Genetic Vectors; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000494:pharmacology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases