title: Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries.
abstract: Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea.
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Kyung Seok
authors.initials: KS
authors.lastname: Yang
authors.forename: Su A
authors.initials: SA
authors.lastname: Jang
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Sung
authors.forename: Sun Chang
authors.initials: SC
authors.lastname: Kim
mesh_terms: D000440:Butanols / Q000378:metabolism*; D002373:Cyclic AMP Receptor Protein / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D006358:Hot Temperature; D007218:Industrial Microbiology / Q000379:methods*; D016335:Zinc Fingers
chemical_list: D000440:Butanols; D002373:Cyclic AMP Receptor Protein; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins