title: [Clinical implication and association between local and systemic levels of interleukin-1β and interleukin-10 in patients with coronary artery disease].
abstract: OBJECTIVE
  To compare the systemic and local near atherosclerosis lesion levels of pro-inflammatory factor interleukin-1β (IL-1β) and anti-inflammatory factor IL-10 in patients with coronary artery disease (CAD).
  
  
  METHODS
  Plasma samples were collected from 30 individuals without angiographical coronary artery stenosis (control group), 90 patients with CAD (stable angina pectoris, SA, n = 30, unstable angina pectoris/non-ST-segment elevation myocardial infarction, UA/NSTEMI, n = 30 and ST-segment elevation myocardial infarction, STEMI, n = 30). During diagnostic coronary angiography or interventional procedures, systemic samples were obtained from aorta root in all patients (n = 120), local samples from distal of the coronary lesion in patients with CAD (n = 90), and samples from coronary sinus of 14 patients with STEMI. IL-1β and IL-10 were determined by ELISA method.
  
  
  RESULTS
  The result showed systemic levels of IL-1β were lg(-1) (0.97 ± 0.42), lg(-1) (0.98 ± 0.43), lg(-1) (1.21 ± 0.42), lg(-1) (1.30 ± 0.43) ng/L in the control, SA, UA/NSTEMI and STEMI groups, were significantly higher in UA/NSTEMI and STEMI groups compared with the control group (P < 0.05, P < 0.01); systemic IL-10 levels were lg(-1) (0.77 ± 0.29), lg(-1) (0.73 ± 0.45), lg(-1) (0.75 ± 0.35), lg(-1) (1.14 ± 0.36) ng/L in the four groups and was significantly higher in STEMI group than the control group (P < 0.01). The local concentration of IL-1β and IL-10 were similar as the systemic levels in SA group [lg(-1) (0.98 ± 0.41), lg(-1) (0.67 ± 0.47) ng/L], local IL-1β [lg(-1) (1.22 ± 0.48) ng/L] was similar while local IL-10 [lg(-1) (0.89 ± 0.46) ng/L] was significantly higher than the systemic levels in UA/NSTEMI group. The local levels of IL-1β and IL-10 [lg(-1) (1.45 ± 0.45), lg(-1) (1.35 ± 0.31) ng/L] were both significantly higher than the systemic levels in STEMI group (all P < 0.01). The IL-1β levels of systemic, local and coronary sinus in STEMI patients with acute totally occluded left coronary artery [lg(-1) (1.47 ± 0.37), lg(-1) (1.65 ± 0.34), lg(-1) (1.53 ± 0.35)ng/L] and the IL-10 levels [lg(-1) (1.06 ± 0.48), lg(-1) (1.34 ± 0.39), lg(-1) (1.34 ± 0.23) ng/L] were similar. The level of IL-1β in coronary sinus was significantly lower than in culprit lesion (P < 0.05) while IL-10 levels were similar at these two sites (P > 0.05).
  
  
  CONCLUSIONS
  The systemic level of pro-inflammatory marker IL-1β and anti-inflammatory marker IL-10 could not reliably reflect the local inflammatory status near the atherosclerosis plaque locations.
authors.affiliation: Chinese Academy of Medical Sciences, Beijing 100037, China. yan591204@yahoo.com.cn
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Yan
authors.forename: Wen-zheng
authors.initials: WZ
authors.lastname: Li
authors.forename: Han-jun
authors.initials: HJ
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
mesh_terms: D000368:Aged; D060050:Angina, Stable / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D053583:Interleukin-1beta / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood
chemical_list: C508609:IL10 protein, human; D053583:Interleukin-1beta; D016753:Interleukin-10