title: Structures of starches from rice mutants deficient in the starch synthase isozyme SSI or SSIIIa.
abstract: Amylose and amylopectin of rice mutants deficient in a starch synthase (SS) isozyme in the endosperm, either SSI (ΔSSI) or SSIIIa (ΔSSIIIa), were structurally altered from those of their parent (cv. Nipponbare, Np). The amylose content was higher in the mutants (Np, 15.5%; ΔSSI, 18.2%; ΔSSIIIa, 23.6%), and the molar ratio of branched amylose and its side chains was increased. The chain-length distribution of the β-amylase limit dextrins of amylopectin showed regularity, which appeared consistent with the generally accepted cluster structure, and the degrees of polymerization found at the intersections were taken as the boundaries of the B-chain fractions. The mole % of the B(1)-B(3) fractions was changed slightly in ΔSSI, which is consistent with the proposed role of SSI in elongating the external part of clusters. In ΔSSIIIa, a significant increase in the B(1) fraction and a decrease in the B(2) and B(3) fractions were observed. The internal chain length of the B(2) and B(3) fractions appeared to be slightly altered, suggesting that the deficiency in SS affected the actions of branching enzyme(s).
authors.affiliation: Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima City, Kagoshima, Japan. ihanashi@chem.agri.kagoshima-u.ac.jp.
authors.forename: Isao
authors.initials: I
authors.lastname: Hanashiro
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Higuchi
authors.forename: Satomi
authors.initials: S
authors.lastname: Aihara
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujita
mesh_terms: D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013213:Starch / Q000737:chemistry*; D013214:Starch Synthase / Q000737:chemistry* / Q000235:genetics
chemical_list: D007527:Isoenzymes; D013213:Starch; D013214:Starch Synthase