title: Identification of components of the female sex pheromone of the Simao pine caterpillar moth, Dendrolimus kikuchii Matsumura.
abstract: The pine caterpillar moth, Dendrolimus kikuchii Matsumura (Lepidoptera: Lasiocampidae), is a pest of economic importance on pine in southwest China. Three active compounds were detected during analyses of solvent extracts and effluvia sampled by solid phase microextraction (SPME) from virgin female D. kikuchii using gas chromatography (GC) coupled with electroantennographic (EAG) recording with antennae from a male moth. The compounds were identified as (5Z,7E)-5,7-dodecadien-1-yl acetate (Z5,E7-12:OAc), (5Z,7E)-5,7-dodecadien-1-ol (Z5,E7-12:OH), and (5Z)-5-dodecenyl acetate (Z5-12:OAc) by comparison of their GC retention indices, mass spectra, and EAG activities with those of synthetic standards. Microchemical reactions of gland extracts provided further information confirming the identifications of the three components. Solvent extractions and SPME samples of pheromone effluvia from virgin calling females provided 100:18:0.6 and 100:7:1 ratios of Z5,E7-12:OAc:Z5,E7-12:OH:Z5-12:OAc, respectively. Field behavioral assays showed that Z5,E7-12:OAc and Z5,E7-12:OH were essential for attraction of male D. kikuchii moths. However, the most attractive blend contained these three components in a 100:20:25 ratio in a gray rubber septa. Our results demonstrated that the blend of Z5,E7-12:OAc, Z5,E7-12:OH, and Z5-12:OAc comprise the sex pheromone of D. kikuchii. The optimized three-component lure blend is recommended for monitoring D. kikuchii infestations.
authors.affiliation: Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, State Forestry Administration, Beijing, 100091, China.
authors.forename: Xiang-Bo
authors.initials: XB
authors.lastname: Kong
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Sun
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Cheng-Hua
authors.initials: CH
authors.lastname: Zhao
authors.forename: Kees C J H
authors.initials: KC
authors.lastname: Booij
mesh_terms: D000085:Acetates / Q000737:chemistry* / Q000302:isolation & purification*; D000818:Animals; D002849:Chromatography, Gas; D005233:Fatty Alcohols / Q000737:chemistry* / Q000302:isolation & purification*; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D009036:Moths / Q000033:anatomy & histology / Q000737:chemistry; D012724:Sex Attractants / Q000032:analysis / Q000737:chemistry; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D000085:Acetates; D005233:Fatty Alcohols; D012724:Sex Attractants; D055549:Volatile Organic Compounds