title: [Impact of hypoxia-inducible factor-1 on tumor invasion and metastasis in breast cancer].
abstract: OBJECTIVE
  To investigate HIF-1α gene expression in human breast cancer cells and the role of HIF-1 in tumor metastasis and invasion.
  
  
  METHODS
  Human breast cancer cell lines were cultured under normoxic or hypoxic conditions. Western blot was used to evaluate the protein expression of HIF-1α, p-vimentin and α-tubulin. Small interfering RNA targeting HIF-1α was used to block the expression of HIF-1α in MDA-MB-231 and SK-BR-3 cell lines. Matrigel transwell and cell wound healing assays were used to detect the capability of cellular invasion and migration, respectively.
  
  
  RESULTS
  Under normoxic condition, all the cell lines tested showed a base-level of HIF-1α expression. The highest expression level of HIF-1α protein was obtained in the MDA-MB-231 cell line, which is also noticed to be highly invasive and migratory in behavior. HIF-1α siRNA was capable of blocking the protein expression of both HIF-1α and p-vimentin and in addition, the attenuated ability of invasion and migration.
  
  
  CONCLUSIONS
  Since HIF-1 is able to promote invasion and metastasis of tumor cells, which may be considered as a target in anti-cancer therapy.
authors.affiliation: Department of Pathology, Inner Mongolia Medical College, Hohhot 010059, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Miao-miao
authors.initials: MM
authors.lastname: Chang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Li-li
authors.initials: LL
authors.lastname: Jiang
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Shi
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000235:genetics*; D014746:Vimentin / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D034741:RNA, Small Interfering; D014746:Vimentin