title: Inhibition of Recombinant D-Amino Acid Oxidase from Trigonopsis variabilis by Salts.
abstract: Inhibition of recombinant D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) activity in the presence of different sodium salts and potassium chloride is reported. A competitive inhibition pattern by sodium chloride was observed, and an inhibition constant value of K(i) = 85 mM was calculated. Direct connection of NaCl inhibition with FAD cofactor dissociation was confirmed by measuring the fluorescence of tryptophanyl residues of the holoenzyme.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, José Antonio Novais 2, 28040 Madrid, Spain.
authors.forename: Jessica
authors.initials: J
authors.lastname: Kopf
authors.forename: Daniel
authors.initials: D
authors.lastname: Hormigo
authors.forename: José Luis
authors.initials: JL
authors.lastname: García
authors.forename: Carmen
authors.initials: C
authors.lastname: Acebal
authors.forename: Isabel
authors.initials: I
authors.lastname: de la Mata
authors.forename: Miguel
authors.initials: M
authors.lastname: Arroyo