title: Molecular characterization of miiuy croaker CC chemokine gene and its expression following Vibrio anguillarum injection.
abstract: A CC chemokine gene was isolated from miiuy croaker (Miichthys miiuy) by expressed sequence tag analysis. The Mimi-CC cDNA contains an open reading frame of 429 nucleotides encoding 142 amino acid residues. The deduced Mimi-CC possesses the typical arrangement of four cysteines as found in other known CC chemokines (C³¹, C³², C⁵⁶, and C⁷⁰). It shares 15.3%-37.4% identity to CC chemokines of mammal and teleost. Phylogenetic analysis showed that miiuy croaker was most closely related to Atlantic cod. Genomic analysis revealed that Mimi-CC gene consists of four exons and three introns, which is not typical of CC chemokines but resembles that of CXC chemokines. Real-time quantitative RT-PCR demonstrated that Mimi-CC is constitutively expressed in most tissues including lymphoid organs, and the highest expression of Mimi-CC transcripts in normal tissues was observed in muscle. Challenge of miiuy croaker with Vibrio anguillarum resulted in significant changes in the expression of CC chemokine transcripts in four tissues, especially in kidney and spleen.
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, Zhejiang province 316000, PR China.
authors.forename: Yuan-zhi
authors.initials: YZ
authors.lastname: Cheng
authors.forename: Ri-xin
authors.initials: RX
authors.lastname: Wang
authors.forename: Yue-na
authors.initials: YN
authors.lastname: Sun
authors.forename: Tian-jun
authors.initials: TJ
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019742:Chemokines, CC / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007438:Introns; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014733:Vibrio / Q000502:physiology*; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*
chemical_list: D019742:Chemokines, CC; D018076:DNA, Complementary; D029941:Fish Proteins