title: [Cellular components of crescents in four common types of crescentic glomerulonephritis].
abstract: OBJECTIVE
  To examine the cellular components at different stages of the crescent formation in four most common types of human crescentic glomerulonephritis (CGN), including anti-GBM disease (GBM-CGN), crescentic IgA nephropathy (IgA-CGN), ANCA associated pauci-immune CGN (ANCA-CGN) and crescentic lupus glomerulonephritis (LN-CGN).
  
  
  METHODS
  Renal biopsy specimens of patients with GBM-CGN (n = 10), IgA-CGN (n = 12), ANCA-CGN (n = 12), and LN-CGN (n = 11) were selected. Immunohistochemistry was adopted to identify the cellular components using different cell markers including cytokeratin (PEC), CD68 (macrophage), nestin (podocyte), podocalyxin (podocyte), CD3 (lymphocyte), CD15 (neutrophil) and PCNA.
  
  
  RESULTS
  There were different subtypes of cell components identified during the formation of a cellular crescent in 4 different types of human CGN. Mainly of PEC 11.4 (0.0, 95.0)%, macrophage 8.0 (0.0, 35.0)% and podocyte 5.5 (0.0, 22.0)% and their constitutive percentages were different among various CGNs (P < 0.01). In all the CGNs studied, there were 50% of cells were negative to all the cell markers adopted for this expeiment. Podocalyxin positive cells 0.5 (0.0, 9.6)% were significantly less than nestin positive cells 5.5 (0.0, 22.0)% in all CGNs. PCNA positive cells were 44.7 (16.7, 83.3)% in the cellular crescent of all CGNs and co-localized with nestin (38/45 cases), CK (42/45 cases) or CD68 (24/45 cases).
  
  
  CONCLUSIONS
  PEC, macrophage and podocyte might play important roles in the formation of crescents. The staining disparity of nestin and podocalyxin indicates that podocyte dedifferentiation may occur during the crescent formation. PEC, podocytes and macrophages may participate in the formation of crescent in common CGNs through active cellular proliferation.
authors.affiliation: Department of Nephrology, Peking University First Hospital, Peking University Institute of Nephrology, Beijing 100034, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Wan-zhong
authors.initials: WZ
authors.lastname: Zou
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: E
authors.forename: Su-xia
authors.initials: SX
authors.lastname: Wang
authors.forename: Ming-hui
authors.initials: MH
authors.lastname: Zhao
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D019867:Anti-Glomerular Basement Membrane Disease / Q000378:metabolism / Q000473:pathology; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005921:Glomerulonephritis / Q000145:classification / Q000378:metabolism / Q000473:pathology*; D005922:Glomerulonephritis, IGA / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D008181:Lupus Nephritis / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D050199:Podocytes / Q000378:metabolism* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D000097382:CD68 Molecule
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D007381:Intermediate Filament Proteins; D007633:Keratins; D009419:Nerve Tissue Proteins; D064231:Nestin; D018809:Proliferating Cell Nuclear Antigen; D012795:Sialoglycoproteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C577484:NES protein, human; C001643:podocalyxin