title: [Pathology of lymphoid tissue cells infected by African swine fever virus in vitro].
abstract: The authors studied the pathology of bone marrow (BM) lymphoid cell from pigs infected by African swine fever virus (ASFV) in vitro. Monocytes were shown to be primarily afflicted in unstimulated BM culture. These cells disappeared completely 72 hours after infection. Just 24 hours following ASFV infection, there were atypical lymphocytes amounting to 12% of the general lymphoid population at hour 72 after inoculation.The area and perimeter of minor, middle, and large lymphocytes tended to reduce during both BM cell cultivation and inoculation. Lymphoblasts and monocytes were generally triploid in both the control and test groups, but among them there were diploid, triploid, and tetraploid cells. Cytophotometric assay revealed that the amount of nuclear DNA significantly increased in BM lymphoblasts and monocytes in the early stages of ASFV infection (within 24 hours). This effect was also rather pronounced in the lymphoblasts in the later stages (at hour 72).
authors.forename: E M
authors.initials: EM
authors.lastname: Karalova
authors.forename: G E
authors.initials: GE
authors.lastname: Voskanian
authors.forename: Kh V
authors.initials: KhV
authors.lastname: Sarkisian
authors.forename: L O
authors.initials: LO
authors.lastname: Abroian
authors.forename: A S
authors.initials: AS
authors.lastname: Avetisian
authors.forename: L A
authors.initials: LA
authors.lastname: Akopian
authors.forename: Z B
authors.initials: ZB
authors.lastname: Semerdzhian
authors.forename: O S
authors.initials: OS
authors.lastname: Zakarian
authors.forename: G A
authors.initials: GA
authors.lastname: Arzumanian
authors.forename: Z A
authors.initials: ZA
authors.lastname: Karalian
mesh_terms: D000357:African Swine Fever / Q000276:immunology / Q000473:pathology* / Q000821:virology; D000358:African Swine Fever Virus / Q000502:physiology; D000818:Animals; D017209:Apoptosis; D001853:Bone Marrow / Q000473:pathology; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000473:pathology; D048430:Cell Shape; D004247:DNA / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology*; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology*; D009000:Monocytes / Q000276:immunology / Q000473:pathology*; D013552:Swine; D057891:Tetraploidy; D013997:Time Factors; D057885:Triploidy
chemical_list: D004247:DNA