title: Production of caloxanthin 3'-β-D-glucoside, zeaxanthin 3,3'-β-D-diglucoside, and nostoxanthin in a recombinant Escherichia coli expressing system harboring seven carotenoid biosynthesis genes, including crtX and crtG.
abstract: The aim of this study was to produce rare β-carotene-modified carotenoids possessing 2-O (-H or -glu) and/or 3-O (-H or -glu) functionalities in their β-ionone ring(s) using a recombinant Escherichia coli approach. This involved expressing seven carotenoid biosynthesis genes (crtE, crtB, crtI, crtY, crtZ, crtX and crtG). From the cells of the recombinant E. coli, caloxanthin (β,β-carotene-2,3,2',3'-tetrol)-3'-β-D-glucose, zeaxanthin (β,β-carotene-3,3'-diol) 3,3'-β-D-diglucoside, and nostoxanthin (β,β-carotene-2,3,3'-triol) (rare carotenoids) were isolated and identified. Caloxanthin 3'-β-D-glucose displayed potent (1)O(2) quenching activity (IC(50) 19 μM).
authors.affiliation: Department of Food and Nutrition, Japan Women's University, Tokyo, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Osawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Harada
authors.forename: Seon-Kang
authors.initials: SK
authors.lastname: Choi
authors.forename: Norihiko
authors.initials: N
authors.lastname: Misawa
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shindo
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002338:Carotenoids / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015394:Molecular Structure; D013237:Stereoisomerism; D024341:Xanthophylls / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005960:Glucosides; D024341:Xanthophylls; C559963:caloxanthin 3'-beta-D-glucoside; C533811:nostoxanthin; C559962:zeaxanthin 3,3'-beta-D-diglucoside; D002338:Carotenoids