title: Evaluation of the mean corpuscular volume of peripheral blood mononuclear cells of HIV patients by a coulter counter to determine intracellular drug concentrations.
abstract: The mean corpuscular volume (MCV) of peripheral blood mononuclear cells (PBMCs) was determined by Coulter Counter, and data were used to calculate the intracellular drug concentrations. A total of 574 PBMC samples were collected from 190 patients. The MCV was 282.9 fl (minimum, 207.0; maximum, 354.6), with a standard deviation of 8.8%. Previous reports have often used a fixed value of 400 fl for the MCV, which may result in artificially low estimates of the intracellular concentrations of antivirals.
authors.affiliation: Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149 Turin, Italy. marco.simiele@unito.it
authors.forename: Marco
authors.initials: M
authors.lastname: Simiele
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Avolio
authors.forename: Lorena
authors.initials: L
authors.lastname: Baietto
authors.forename: Marco
authors.initials: M
authors.lastname: Siccardi
authors.forename: Mauro
authors.initials: M
authors.lastname: Sciandra
authors.forename: Silvia
authors.initials: S
authors.lastname: Agati
authors.forename: Jessica
authors.initials: J
authors.lastname: Cusato
authors.forename: Stefano
authors.initials: S
authors.lastname: Bonora
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Perri
mesh_terms: D000328:Adult; D000368:Aged; D000998:Antiviral Agents / Q000627:therapeutic use; D004909:Erythrocyte Indices; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D000998:Antiviral Agents