title: Biosynthesis of the RNA polymerase inhibitor streptolydigin in Streptomyces lydicus: tailoring modification of 3-methyl-aspartate.
abstract: The asparaginyl-tRNA synthetase-like SlgZ and methyltransferase SlgM enzymes are involved in the biosynthesis of the tetramic acid streptolydigin in Streptomyces lydicus. Inactivation of slgZ led to a novel streptolydigin derivative. Overexpression of slgZ, slgM, or both in S. lydicus led to a considerable increase in streptolydigin production.
authors.affiliation: Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.
authors.forename: Dina H
authors.initials: DH
authors.lastname: Horna
authors.forename: Cristina
authors.initials: C
authors.lastname: Gómez
authors.forename: Carlos
authors.initials: C
authors.lastname: Olano
authors.forename: Martina
authors.initials: M
authors.lastname: Palomino-Schätzlein
authors.forename: Antonio
authors.initials: A
authors.lastname: Pineda-Lucena
authors.forename: Rodrigo J
authors.initials: RJ
authors.lastname: Carbajo
authors.forename: Alfredo F
authors.initials: AF
authors.lastname: Braña
authors.forename: Carmen
authors.initials: C
authors.lastname: Méndez
authors.forename: José A
authors.initials: JA
authors.lastname: Salas
mesh_terms: D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis*; D001224:Aspartic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics*; D017353:Gene Deletion; D015870:Gene Expression; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000617:Aminoglycosides; D001426:Bacterial Proteins; D001224:Aspartic Acid; C100307:streptolydigin