title: Bacteriophages LIMElight and LIMEzero of Pantoea agglomerans, belonging to the "phiKMV-like viruses".
abstract: Pantoea agglomerans is a common soil bacterium used in the biocontrol of fungi and bacteria but is also an opportunistic human pathogen. It has been described extensively in this context, but knowledge of bacteriophages infecting this species is limited. Bacteriophages LIMEzero and LIMElight of P. agglomerans are lytic phages, isolated from soil samples, belonging to the Podoviridae and are the first Pantoea phages of this family to be described. The double-stranded DNA (dsDNA) genomes (43,032 bp and 44,546 bp, respectively) encode 57 and 55 open reading frames (ORFs). Based on the presence of an RNA polymerase in their genomes and their overall genome architecture, these phages should be classified in the subfamily of the Autographivirinae, within the genus of the "phiKMV-like viruses." Phylogenetic analysis of all the sequenced members of the Autographivirinae supports the classification of phages LIMElight and LIMEzero as members of the "phiKMV-like viruses" and corroborates the subdivision into the different genera. These data expand the knowledge of Pantoea phages and illustrate the wide host diversity of phages within the "phiKMV-like viruses."
authors.affiliation: Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Box 2462, Leuven B-3001, Belgium.
authors.forename: Evelien M
authors.initials: EM
authors.lastname: Adriaenssens
authors.forename: Pieter-Jan
authors.initials: PJ
authors.lastname: Ceyssens
authors.forename: Vincent
authors.initials: V
authors.lastname: Dunon
authors.forename: Hans-Wolfgang
authors.initials: HW
authors.lastname: Ackermann
authors.forename: Johan
authors.initials: J
authors.lastname: Van Vaerenbergh
authors.forename: Martine
authors.initials: M
authors.lastname: Maes
authors.forename: Maurice
authors.initials: M
authors.lastname: De Proft
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
mesh_terms: D001433:Bacteriolysis; D001435:Bacteriophages / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D016000:Cluster Analysis; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D023061:Gene Order; D008854:Microscopy, Electron; D016366:Open Reading Frames; D020636:Pantoea / Q000821:virology*; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012988:Soil Microbiology; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D004247:DNA