title: A simple osmotic stress test to predict boar sperm cryosurvival.
abstract: This work was carried out to test whether viability of pig spermatozoa subjected to an osmotic test is correlated to sperm cryosurvival. Spermatozoa were cooled from 22 degrees C to -5 degrees C, aliquots were exposed to a series of hyperosmotic solutions (300-2100 mOsm/kg) for 15 min, immediately spermatozoa were re-warmed to 37 degrees C and isosmolarity was restored. Spermatozoa were cooled from 22 degrees C to -5 degrees C and one aliquot was exposed to the osmotic test while diluted spermatozoa were frozen-thawed. Plasma membrane-intact spermatozoa decreased as osmolarity increased (P < 0.0001), a further decreased (P < 0.0001) was observed when isotonicity was restored. Proportions of plasma membrane-intact and acrosome-intact cells from the osmotic test were no different from those after freeze-thawing: 36% vs. 35%, 80% vs. 80%, respectively. A significant correlation was found between the proportion of acrosome-intact cells after freeze-thawing and that from the osmotic test (r = 0.81, P <0.01). This test provides a useful and economical mean to predict in vitro boar sperm cryosurvival.
authors.affiliation: Departamento de Ciencias Pecuarias, Facultad de Estudios Superiores--Cuautitlan, Universidad Nacional Autonoma de Mexico, Carretera Cuautitlan -Teoloyucan s/n. Cuautitlan Izcalli, Estado de Mexico, 54714. Mexico.
authors.forename: Cesar
authors.initials: C
authors.lastname: Garzon-Perez
authors.forename: Hector F
authors.initials: HF
authors.lastname: Flores
authors.forename: Alfredo
authors.initials: A
authors.lastname: Medrano
mesh_terms: D000818:Animals; D002462:Cell Membrane; D002470:Cell Survival; D003080:Cold Temperature; D015925:Cryopreservation; D003584:Cytological Techniques; D005615:Freezing; D008297:Male; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D011237:Predictive Value of Tests; D012662:Semen Preservation / Q000379:methods; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa; D013552:Swine