title: [Determination of TTX in biological samples by LC-MS/MS].
abstract: OBJECTIVE
  To propose a method for determination of tetrodotoxin (TTX) in human blood, urine and liver by liquid chromatography with tandem mass spectrometry (LC-MS/MS).
  
  
  METHODS
  Solid phase extraction is used after the samples are precipitated, then the samples will be analyzed by LC-MS/MS.
  
  
  RESULTS
  The limits of detection were 2 ng/mL in blood and urine and 4 ng/g in liver for TIX respectively, the linear correlation coefficients were not less than 0.9973, both of the intra-day and inter-day precisions were less than 12.80%, the recoveries for all kinds of specimens were more than 47.2%.
  
  
  CONCLUSIONS
  This method is efficient, sensitive and accurate and was successfully validated for implementation in forensic toxicological analysis.
authors.affiliation: Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. darcy5358@sina.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Da
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Shen
authors.forename: Min
authors.initials: M
authors.lastname: Shen
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D053593:Forensic Toxicology; D006801:Humans; D008099:Liver / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D013048:Specimen Handling / Q000379:methods; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013779:Tetrodotoxin / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D013779:Tetrodotoxin