title: Epidemiological and clinical characteristics of cutaneous leishmaniasis and their relationship with the laboratory data, south of Brazil.
abstract: OBJECTIVE
  To evaluate clinical, epidemiological and laboratorial aspects for the understanding of the disease characteristics and its relationship with diagnostic tests.
  
  
  METHODS
  A retrospective, descriptive and analytical study involving 2,660 American cutaneous leishmaniasis (ACL) suspected patients from southern Brazil was undertaken between April 1986 and December 2005. Data on population characteristics and laboratory tests were obtained. Diagnostic laboratory tests used were direct search for Leishmania spp. (DS), Montenegro skin test (MST) and indirect immunofluorescent assay (IFA).
  
  
  RESULTS
  62.3% of patients were positive for at least one laboratory test. DS test was positive in 65.1%; MST in 92.3% and IFA in 70.0%. Although Cohen's Kappa test did not reveal any agreement with laboratory diagnosis for ACL, the association between MST and IFA tests increased positiveness to 98.9%; MST and DS to 97.2%; and IFA and DS to 85%. IFA and MST positiveness were higher among patients with < 2-month lesion-evolution time. Most ACL patients were male (72.6%) in the age range of 15-49 years, featuring lesions during two months or less (53.9%) and a cutaneous form of the disease (88.0%).
  
  
  CONCLUSIONS
  Findings suggest that for the adequate identification of ACL cases a combination of laboratory tests that involves the association of MST with at least another test should be used.
authors.affiliation: Universidade Estadual de Maringá.
authors.forename: Maura Cristiani de Moura
authors.initials: MC
authors.lastname: Curti
authors.forename: Thaís Gomes Verzignassi
authors.initials: TG
authors.lastname: Silveira
authors.forename: Sandra Mara Alessi Aristides
authors.initials: SM
authors.lastname: Arraes
authors.forename: Dennis Armando
authors.initials: DA
authors.lastname: Bertolini
authors.forename: Paulo Donizeti
authors.initials: PD
authors.lastname: Zanzarini
authors.forename: Eneide Aparecida Sabaini
authors.initials: EA
authors.lastname: Venazzi
authors.forename: Andrea Cláudia Silva
authors.initials: AC
authors.lastname: Fernandes
authors.forename: Jorge Juarez Vieira
authors.initials: JJ
authors.lastname: Teixeira
authors.forename: Maria Valdrinez Campana
authors.initials: MV
authors.lastname: Lonardoni
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000453:epidemiology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012424:Rural Population; D012882:Skin Tests; D014505:Urban Population; D055815:Young Adult