title: Enzyme immobilization by entrapment.
abstract: Entrapment methods of immobilization are mostly used in cell immobilization procedures, but have found some application with enzymes, particularly when the enzyme is essentially a dead cell or crude homogenate(1). In theory the entrapped enzyme is not attached to the polymer; its free diffusion is merely restrained. In practice, however, some or all of the enzyme may be an integral part of the polymer matrix; for example, chain termination in polyacrylamide gel formation may occur onto the enzyme (see also ref. 2).
authors.affiliation: Biological Sciences, The Hatfield Polytechnic, Hatfield, Hertfordshire, UK.
authors.forename: M D
authors.initials: MD
authors.lastname: Trevan