title: Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension.
abstract: Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: Treuer
authors.forename: Shelley E
authors.initials: SE
authors.lastname: Haydel
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry; D003264:Containment of Biohazards; D009161:Mycobacterium / Q000737:chemistry* / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents