title: Clinical evaluation of Oxoid Brilliance MRSA Agar in comparison with bioMerieux MRSA ID medium for detection of livestock-associated meticillin-resistant Staphylococcus aureus.
abstract: Oxoid Brilliance MRSA Agar and bioMérieux MRSA ID medium were evaluated for their ability to identify meticillin-resistant Staphylococcus aureus (MRSA) in clinical samples. Nasal and throat samples (n = 629) were taken from veterinarians and their household members. The sensitivities of Brilliance MRSA Agar and MRSA ID medium after 20 h of incubation were 63.6 and 64.5 %, and the specificities were 94.1 and 99.4 %, respectively. After an enrichment step, the sensitivities increased to 96.3 and 97.2 %, but the specificities decreased to 88.7 and 98.5 %, respectively. Brilliance MRSA Agar and MRSA ID medium are both sensitive methods for the screening of MRSA in combination with broth enrichment, but positive results require confirmation.
authors.affiliation: Laboratory for Medical Microbiology and Immunology, St Elisabeth Hospital, Tilburg, The Netherlands.|Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
authors.forename: Erwin
authors.initials: E
authors.lastname: Verkade
authors.affiliation: Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
authors.forename: Marianne
authors.initials: M
authors.lastname: Ferket
authors.affiliation: Department of Medical Microbiology and Infection Prevention, VU University Medical Center, Amsterdam, The Netherlands.|Laboratory for Medical Microbiology and Immunology, St Elisabeth Hospital, Tilburg, The Netherlands.|Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: Kluytmans
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D058751:Livestock / Q000382:microbiology; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D009666:Nose / Q000382:microbiology; D009784:Occupational Diseases / Q000382:microbiology*; D016273:Occupational Exposure; D010614:Pharynx / Q000382:microbiology; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*; D013552:Swine; D018634:Veterinarians
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D008712:Methicillin