title: Chromosome 11q23.1 is an unstable region in B-cell tumor cell lines.
abstract: Chromosome 11q23 region is a frequent target of chromosome aberrations in B-cell lymphoid tumors. Here, we present the cytogenetic and molecular characterization of an amplification affecting 11q23.1 in four cell lines derived from B-cell lymphoid tumors. A minimal common region of amplification of 330 kb was identified in three cell lines using Affymetrix Human Mapping 250K arrays. When analyzed with three BAC clones, the amplifications appeared different at cytogenetic level in each cell line. Possibly affected transcripts were evaluated using tiling arrays, and validated by real time PCR. Since no effect of the amplification at the local transcription level was observed, it is possible that 11q23 amplification might mainly represent the effect of unstable chromosomal region.
authors.affiliation: Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland.
authors.forename: Giulia
authors.initials: G
authors.lastname: Poretti
authors.forename: Ivo
authors.initials: I
authors.lastname: Kwee
authors.forename: Barbara
authors.initials: B
authors.lastname: Bernasconi
authors.forename: Paola M V
authors.initials: PM
authors.lastname: Rancoita
authors.forename: Andrea
authors.initials: A
authors.lastname: Rinaldi
authors.forename: Carlo
authors.initials: C
authors.lastname: Capella
authors.forename: Emanuele
authors.initials: E
authors.lastname: Zucca
authors.forename: Antonino
authors.initials: A
authors.lastname: Neri
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Tibiletti
authors.forename: Francesco
authors.initials: F
authors.lastname: Bertoni
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D005784:Gene Amplification; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009369:Neoplasms / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D054648:Protein Phosphatase 2 / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics
chemical_list: C091546:POU2AF1 protein, human; C432785:PPP2R1B protein, human; D015534:Trans-Activators; C551842:salt-inducible kinase-2, human; D017346:Protein Serine-Threonine Kinases; D054648:Protein Phosphatase 2