title: Characterization of a new α-L: -arabinofuranosidase from Penicillium sp. LYG 0704, and their application in lignocelluloses degradation.
abstract: A gene (arf) encoding an α-L: -arabinofuranosidase (ARF) that hydrolyzes arabinose substituted on xylan was isolated from Penicillium sp. The gene was predicted to encode 339 amino acid residues showing 71-75% homology to GH family 54. E. coli expressed ARF showed optimal activity at 50°C and pH 5-6 on wheat arabinoxylan. The hydrolysis activities on oat spelt xylan by ARF and xylanase were 1.67-fold higher than that of xylanase alone. The synergistic effects of ARF and commercial enzymes (xylanase and cellulase) on popping-pretreated rice straw were 1.15-1.51-fold higher amounts of sugars released in the [ARF + xylanase + cellulase] mixture than in the mixtures [ARF + xylanase], [ARF + cellulase], and [xylanase + cellulase]. Moreover, the liberation of arabinose by ARF was enhanced 2.1-2.9-fold in a reaction with xylanase and cellulase as compared with [xylanase + cellulase] and ARF alone.
authors.affiliation: Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Dae-Seok
authors.initials: DS
authors.lastname: Lee
authors.forename: Seung-Gon
authors.initials: SG
authors.lastname: Wi
authors.forename: Yoon-Gyo
authors.initials: YG
authors.lastname: Lee
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Cho
authors.forename: Byung-Yeoup
authors.initials: BY
authors.lastname: Chung
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D000595:Amino Acid Sequence; D001089:Arabinose / Q000032:analysis; D018554:Avena / Q000378:metabolism; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000032:analysis; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D014866:Waste Products; D014990:Xylans / Q000378:metabolism / Q000648:ultrastructure; D014994:Xylose / Q000032:analysis
chemical_list: D014866:Waste Products; D014990:Xylans; C036909:lignocellulose; D008031:Lignin; D014994:Xylose; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase; D043364:Endo-1,4-beta Xylanases; D005947:Glucose