title: Protective effect of structurally diverse grape procyanidin fractions against UV-induced cell damage and death.
abstract: UV radiation leads to the generation of reactive oxygen species (ROS). These molecules exert a variety of harmful effects by altering key cellular functions and may result in cell death. Several studies have demonstrated that human skin can be protected against UV radiation by using plant-derived antioxidants. Here we evaluated the in vitro capacity of several antioxidant polyphenolic fractions from grape, which differ in their degree of polymerization and percentage of galloylation, to protect HaCaT human keratinocytes against UV-induced oxidative damage. These fractions inhibited both basal and UVB- or UVA-induced intracellular ROS generation in this cell line. Consequently, the same fractions inhibited p38 and JNK1/2 activation induced by UVB or UVA radiation. The highest protective effect was for fractions rich in procyanidin oligomers and gallate esters. These encouraging in vitro results support further research and should be taken into consideration into the clinical pharmacology of plant-derived polyphenolic extracts as novel agents for skin photoprotection.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona and IBUB, Unit Associated with CSIC, Barcelona, Spain.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Matito
authors.forename: Neus
authors.initials: N
authors.lastname: Agell
authors.forename: Susana
authors.initials: S
authors.lastname: Sanchez-Tena
authors.forename: Josep L
authors.initials: JL
authors.lastname: Torres
authors.forename: Marta
authors.initials: M
authors.lastname: Cascante
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D044946:Biflavonoids / Q000737:chemistry / Q000494:pharmacology*; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects* / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D058105:Polymerization; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; D020011:Protective Agents; C017674:procyanidin; D002392:Catechin