title: Structural change of a cofactor binding site of flavoprotein detected by single-protein fluorescence spectroscopy at 1.5 k.
abstract: The visible fluorescence spectrum of single flavoprotein at a temperature of 1.5 K has been measured by one-photon excitation. The flavoprotein studied was a photoswitchable enzyme, photoactivated adenylyl cyclase. The time course of the spectrum revealed a structural change occurring at a rate of 10(-3)  s(-1) around hydrogen bonds at the flavin cofactor binding site.
authors.affiliation: Department of Physics, Tokyo Institute of Technology, Meguro, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Fujiyoshi
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Hirano
authors.forename: Michio
authors.initials: M
authors.lastname: Matsushita
authors.forename: Mineo
authors.initials: M
authors.lastname: Iseki
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D001665:Binding Sites; D005056:Euglena gracilis; D005415:Flavins / Q000378:metabolism*; D005420:Flavoproteins / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D017785:Photons; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
chemical_list: D005415:Flavins; D005420:Flavoproteins; D015800:Protozoan Proteins