title: Glutathione peroxidase (GSHPx) activity in the liver of red deer in relation to hepatic selenium concentrations, sex, body weight and season of the year.
abstract: The purpose of this study was to determine glutathione peroxidase (GSHPx) activity in the liver of red deer in relation to selenium concentrations in the liver, as well as to evaluate changes in GSHPx activity according to sex, body weight and season of the year. Total selenium concentration in the liver of red deer averaged 0.095 ± 0.018 μg/g of wet weight. GSHPx activity in the liver of red deer ranged widely from 4.4 to 45.8 U/g of protein. Females were characterized by higher GSHPx activity compared to males (21.2 vs. 17.0 U/g protein). The highest GSHPx activity was recorded in autumn and the lowest in summer. The lowest GSHPx activity in the liver was found in the heaviest animals (>100 kg body weight), averaging 14.0 U/g protein. Animals weighing <66 kg and 66-100 kg were characterized by similar activity of 25.1 and 24.5 U/g, respectively. Despite the differences in GSHPx activity according to sex, body weight and season of the year, these factors had no significant effect on the activity of this enzyme. The main factor regulating GSHPx activity in the liver of examined red deer was selenium concentration.
authors.affiliation: Department of Animal Reproduction Biotechnology and Environmental Hygiene, West Pomeranian University of Technology in Szczecin, Judyma 6 Street, 71-466, Szczecin, Poland. Bogumila.Pilarczyk@zut.edu.pl
authors.forename: Bogumiła
authors.initials: B
authors.lastname: Pilarczyk
authors.forename: Radosław
authors.initials: R
authors.lastname: Drozd
authors.forename: Renata
authors.initials: R
authors.lastname: Pilarczyk
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Tomza-Marciniak
authors.forename: Dorota
authors.initials: D
authors.lastname: Jankowiak
authors.forename: Diana
authors.initials: D
authors.lastname: Hendzel
authors.forename: Jarosław
authors.initials: J
authors.lastname: Kuba
authors.forename: Joanna
authors.initials: J
authors.lastname: Kowalska
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology*; D003627:Data Interpretation, Statistical; D003670:Deer / Q000502:physiology*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007202:Indicators and Reagents; D008099:Liver / Q000201:enzymology* / Q000378:metabolism*; D008297:Male; D012621:Seasons; D012643:Selenium / Q000378:metabolism*; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal; D013050:Spectrometry, Fluorescence
chemical_list: D007202:Indicators and Reagents; D005979:Glutathione Peroxidase; D012643:Selenium