title: Three novel antimicrobial peptides from the skin of the Indian bronzed frog Hylarana temporalis (Anura: Ranidae).
abstract: Amphibian skin secretion is considered as a rich source of bioactive peptides. The present work describes the successful identification of three novel peptides named brevinin-1TEa, brevinin-2TEa and brevinin-2TEb present in the skin secretion of Indian bronzed frog Hylarana temporalis. The deduced open reading frame encoding the biosynthetic precursor of brevinin-1TEa consisted of 70 amino acid residues and brevinin-2TEa and brevinin-2TEb consisted of 71 and 72 amino acids, respectively. All the three peptides showed higher antimicrobial activity against Gram-negative than against Gram-positive bacteria. On the basis of the antibacterial and haemolytic activity, brevinin-2TEb is the most potent peptide reported in the present study. Further research on these peptides may provide potential clue towards newer drug development to combat various microbial diseases.
authors.affiliation: Chemical Biology Group, Molecular Medicine Division, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram 695014, Kerala, India.
authors.forename: V
authors.initials: V
authors.lastname: Reshmy
authors.forename: V
authors.initials: V
authors.lastname: Preeji
authors.forename: A
authors.initials: A
authors.lastname: Parvin
authors.forename: K
authors.initials: K
authors.lastname: Santhoshkumar
authors.forename: S
authors.initials: S
authors.lastname: George
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D001001:Anura; D002942:Circular Dichroism; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D010455:Peptides