title: Development of an operator-independent method for seeding tissue-engineered vascular grafts.
abstract: The manual seeding of cells onto a biodegradable scaffold by pipetting is an effective method of cell seeding. However, the widespread use and ultimate clinical utility of this technique is limited by operator variability. This study was conducted to evaluate an operator-independent vacuum-seeding method for use in an upcoming clinical trial. Using bone marrow-derived mononuclear cells, we achieved seeding comparable to manually seeded scaffolds in terms of cellular attachment, distribution, and viability in vacuum-seeded grafts at vacuum pressures of -25 to -50 mmHg. In conclusion, we describe an operator-independent seeding method for use in the clinical setting.
authors.affiliation: Department of Surgery, Yale University School of Medicine, Yale-New Haven Hospital, New Haven, Connecticut 06520-8062, USA.
authors.forename: Brooks
authors.initials: B
authors.lastname: Udelsman
authors.forename: Narutoshi
authors.initials: N
authors.lastname: Hibino
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Villalona
authors.forename: Edward
authors.initials: E
authors.lastname: McGillicuddy
authors.forename: Alejandro
authors.initials: A
authors.lastname: Nieponice
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Shojiro
authors.initials: S
authors.lastname: Matsuda
authors.forename: David A
authors.initials: DA
authors.lastname: Vorp
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shinoka
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Breuer
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques; D002470:Cell Survival; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D010477:Perfusion; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014618:Vacuum; D058017:Vascular Grafting / Q000379:methods*