title: Use of (35)s nucleotides for DNA sequencing.
abstract: DNA sequencing is a technique that has become central to many laboratories engaged in molecular biology. The two basic sequencing methods commonly used are known as the Maxam-Gilbert method of chemical cleavage (1) and the dideoxy chain termination method developed originally by Sanger (2). One of the most critical steps in either of these two methods is the radiolabeling of the DNA such that the sequencing gel "ladder" of reaction products may be obtained after autoradiography of the sequencing gel.
authors.affiliation: MRC Unit for Molecular and Cellular Cardiology, Department of Medical Physiology and Biochemistry, University of Stellenbosch Medical School, Tygerberg, South Africa.
authors.forename: P D
authors.initials: PD
authors.lastname: Van Helden