title: Applicability of urinary benzene to biological monitoring of occupational and environmental exposure to very low benzene concentrations.
abstract: OBJECTIVE
  To verify whether urinary benzene is an applicable biomarker of occupational exposure to very low concentrations of benzene, considering the influence of cigarette smoke and benzene-toluene co-exposure.
  
  
  METHODS
  23 filling station attendants with occupational exposure to benzene and 31 controls were analyzed. Occupational and environmental exposure was monitored and t,t-muconic acid (t,t-MA), S-phenylmercapturic acid (SPMA), urinary benzene and creatinine in the urine samples were tested.
  
  
  RESULTS
  Occupational exposure to benzene and toluene was significantly higher in the filling station attendants than in the controls, whereas t,t-MA, SPMA and urinary benzene were not different in the two groups. Instead, the smoker group showed significantly higher values for the above biomarkers than the non-smoker group, each of which included both exposed workers and controls. SPMA was dependent on airborne benzene and cigarette smoking, and urinary benzene only on cigarette smoking, while t,t-MA was not dependent on either of these variables.
  
  
  CONCLUSIONS
  At very low concentrations of occupational exposure to benzene, urinary benzene is less valid than SPMA as a biomarker, even if both are strongly influenced by smoking habit. Abstention from smoking should therefore be recommended for at least two hours before urine collection.
authors.affiliation: Dipartimento di Medicina Interna e Medicina Pubblica, Sezione di Medicina del Lavoro E. C. Vigliani, University of Bari, Bari, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Lovreglio
authors.forename: M
authors.initials: M
authors.lastname: Carrieri
authors.forename: A
authors.initials: A
authors.lastname: Barbieri
authors.forename: L
authors.initials: L
authors.lastname: Sabatini
authors.forename: M E
authors.initials: ME
authors.lastname: Fracasso
authors.forename: D
authors.initials: D
authors.lastname: Doria
authors.forename: S
authors.initials: S
authors.lastname: Iavicoli
authors.forename: I
authors.initials: I
authors.lastname: Drago
authors.forename: M N
authors.initials: MN
authors.lastname: D'errico
authors.forename: M
authors.initials: M
authors.lastname: Imbriani
authors.forename: F S
authors.initials: FS
authors.lastname: Violante
authors.forename: G B
authors.initials: GB
authors.lastname: Bartolucci
authors.forename: L
authors.initials: L
authors.lastname: Soleo
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000652:urine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000652:urine*; D000465:Algorithms; D001554:Benzene / Q000378:metabolism* / Q000633:toxicity; D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D003404:Creatinine / Q000652:urine; D004781:Environmental Exposure / Q000032:analysis; D004784:Environmental Monitoring; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000032:analysis*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D014050:Toluene / Q000652:urine
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D014050:Toluene; C005939:muconic acid; C053003:S-phenyl-N-acetylcysteine; D003404:Creatinine; D001554:Benzene; D000111:Acetylcysteine; D013011:Sorbic Acid