title: Identification of QTLs for seed germination capability after various storage periods using two RIL populations in rice.
abstract: Seed germination capability of rice is one of the important traits in the production and storage of seeds. Quantitative trait loci (QTL) associated with seed germination capability in various storage periods was identified using two sets of recombinant inbred lines (RILs) which derived from crosses between Milyang 23 and Tong 88-7 (MT-RILs) and between Dasanbyeo and TR22183 (DT-RILs). A total of five and three main additive effects (QTLs) associated with seed germination capability were identified in MT-RILs and DT-RILs, respectively. Among them, six QTLs were identified repeatedly in various seed storage periods designated as qMT-SGC5.1, qMT-SGC7.2, and qMT-SGC9.1 on chromosomes 5, 7, and 9 in MT-RILs, and qDT-SGC2.1, qDT-SGC3.1, and qDT-SGC9.1 on chromosomes 2, 3, and 9 in DT-RILs, respectively. The QTL on chromosome 9 was identified in both RIL populations under all three storage periods, explaining up to 40% of the phenotypic variation. Eight and eighteen pairs additive × additive epistatic effect (epistatic QTL) were identified in MT-RILs and DT-RILs, respectively. In addition, several near isogenic lines (NILs) were developed to confirm six repeatable QTL effects using controlled deterioration test (CDT). The identified QTLs will be further studied to elucidate the mechanisms controlling seed germination capability, which have important implications for long-term seed storage.
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, China.
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Jiang
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Jin
authors.forename: Yongli
authors.initials: Y
authors.lastname: Qiao
authors.forename: Rihua
authors.initials: R
authors.lastname: Piao
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Jang
authors.forename: Mi-Ok
authors.initials: MO
authors.lastname: Woo
authors.forename: Soon-Wook
authors.initials: SW
authors.lastname: Kwon
authors.forename: Xianhu
authors.initials: X
authors.lastname: Liu
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Pan
authors.forename: Xinglin
authors.initials: X
authors.lastname: Du
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Koh
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D004843:Epistasis, Genetic; D005819:Genetic Markers; D018525:Germination / Q000235:genetics*; D007178:Inbreeding; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D011309:Preservation, Biological / Q000009:adverse effects*; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics* / Q000254:growth & development
chemical_list: D005819:Genetic Markers