title: [Study of the functional role of mutation in the guinea pig-adapted Ebola virus genome on a Drosophila melanogaster model].
abstract: Ebola virus virulence in guinea pigs, which appears through virus adaptation to this animal host, correlates with substitutions in the gene encoding vp24 protein. In particular, the substitution His-->Tyr186 was found when obtaining strain 8 ms. An attempt was made to clarify the functional role of this substitution in a transgenic fruit fly model. Using the drosophila transformation technique provided transgenic strains that contained genomic insertions of wild-type Ebola virus vp24 gene and the mutant gene with the His-->Tyr substitution at the above position. Thus, the drosophila strains carrying the sequences encoding for the vp24 proteins of Ebola virus Zaire and 8 ms in pUAST vector were obtained. This makes it possible to study the expression of transgenic constructs in various D. melanogaster organs and tissues.
authors.forename: A A
authors.initials: AA
authors.lastname: Shelemba-Chepurnova
authors.forename: L V
authors.initials: LV
authors.lastname: Omel'ianchuk
authors.forename: A A
authors.initials: AA
authors.lastname: Chepurnov
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004195:Disease Models, Animal; D004331:Drosophila melanogaster / Q000235:genetics* / Q000821:virology*; D029043:Ebolavirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D005818:Genetic Engineering; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000235:genetics / Q000821:virology; D009154:Mutation; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins