title: Differences in serotoninergic metabolism possibly contribute to differences in breathing phenotype of FVB/N and C57BL/6J mice.
abstract: Mouse readiness for gene manipulation allowed the production of mutants with breathing defects reminiscent of breathing syndromes. As C57BL/6J and FVB/N inbred strains were often used as background strains for producing mutants, we compared their breathing pattern from birth onwards. At birth, in vivo and in vitro approaches revealed robust respiratory rhythm in FVB/N, but not C57BL/6J, neonates. With aging, rhythm robustness difference persisted, and interstrain differences in tidal volume, minute ventilation, breathing regulations, and blood-gas parameters were observed. As serotonin affected maturation and function of the medullary respiratory network, we examined the serotoninergic metabolism in the medulla of C57BL/6J and FVB/N neonates and aged mice. Interstrain differences in serotoninergic metabolism were observed at both ages. We conclude that differences in serotoninergic metabolism possibly contribute to differences in breathing phenotype of FVB/N and C57BL/6J mice.
authors.affiliation: Laboratoire Réponses Cellulaires et Fonctionnelles à l'Hypoxie, EA 2363, UFR Santé, Médecine, Biologie Humaine, Université Paris 13, 74 rue Marcel Cachin, Bureau 128, 93017 BOBIGNY Cedex, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Menuet
authors.forename: Nazim
authors.initials: N
authors.lastname: Kourdougli
authors.forename: Gérard
authors.initials: G
authors.lastname: Hilaire
authors.forename: Nicolas
authors.initials: N
authors.lastname: Voituron
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002245:Carbon Dioxide / Q000378:metabolism; D004195:Disease Models, Animal; D005865:Gestational Age; D006935:Hypercapnia / Q000378:metabolism / Q000503:physiopathology; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology; D008526:Medulla Oblongata / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000378:metabolism; D010507:Periodicity; D010641:Phenotype; D010791:Phrenic Nerve / Q000503:physiopathology; D010991:Plethysmography; D015656:Respiratory Mechanics; D012701:Serotonin / Q000378:metabolism*; D013147:Spirometry; D013990:Tidal Volume
chemical_list: D002245:Carbon Dioxide; D012701:Serotonin; D010100:Oxygen