title: Linarin isolated from Buddleja officinalis prevents hydrogen peroxide-induced dysfunction in osteoblastic MC3T3-E1 cells.
abstract: The flowers and leaves buds of Buddleja officinalis MAXIM (Buddlejaceae) are used to treat eye troubles, hernia, gonorrhea and liver troubles in Asia. To elucidate the protective effects of linarin isolated from B. officinalis on the response of osteoblast to oxidative stress, osteoblastic MC3T3-E1 cells were pre-incubated with linarin for 1h before treatment with 0.3mM H(2)O(2) for 48h, and markers of osteoblast function and oxidative damage were examined. Linarin significantly (P<0.05) increased cell survival, alkaline phosphatase (ALP) activity, collagen content, calcium deposition, and osteocalcin secretion and decreased the production of receptor activator of nuclear factor-kB ligand (RANKL), protein carbonyl (PCO), and malondialdehyde (MDA) of osteoblastic MC3T3-E1 cells in the presence of hydrogen peroxide. These results demonstrate that linarin can protect osteoblasts against hydrogen peroxide-induced osteoblastic dysfunction and may exert anti-resorptive actions, at least in part, via the reduction of RANKL and oxidative damage.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Young Soon
authors.initials: YS
authors.lastname: Lee
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Choi
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D032861:Buddleja / Q000737:chemistry*; D002118:Calcium / Q000032:analysis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D003094:Collagen / Q000032:analysis; D006027:Glycosides / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D010006:Osteoblasts / Q000276:immunology*; D015675:Osteocalcin / Q000032:analysis; D010024:Osteoporosis / Q000276:immunology*; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology; D053245:RANK Ligand / Q000032:analysis
chemical_list: D006027:Glycosides; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D015675:Osteocalcin; D008315:Malondialdehyde; D003094:Collagen; D006861:Hydrogen Peroxide; D000469:Alkaline Phosphatase; C008282:linarin; D002118:Calcium