title: Biological evaluation of phenolic constituents from the trunk of Berberis koreana.
abstract: A bioassay-guided fractionation and chemical investigation of the trunk of Berberis koreana resulted in the isolation and identification of a new sesquilignan, named berbikonol (1), along with fourteen known lignan derivatives (2-15) and a new phenolic compound, named berfussinol (16), together with five known ones (17-21). The structures of these new compounds were elucidated on the basis of 1D and 2D NMR spectroscopic data analysis as well as circular dichroism (CD) spectroscopy studies. Compounds 1-5, 7-8, 11, and 14 showed significant cytotoxicity against the XF498 cell line with IC(50) values of 7.14-19.32 μM. In addition, compounds 3-8 and 15 strongly reduced nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 cells, a microglial cell line.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Jangan-ku, Suwon 440-746, Republic of Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Eunjung
authors.initials: E
authors.lastname: Moon
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D028142:Berberis / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D008070:Lipopolysaccharides / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity
chemical_list: D017705:Lignans; D008070:Lipopolysaccharides; D010636:Phenols; C559407:berbikonol; C559408:berfussinol; D009569:Nitric Oxide