title: Infrared mapping resolves soft tissue preservation in 50 million year-old reptile skin.
abstract: Non-destructive Fourier Transform InfraRed (FTIR) mapping of Eocene aged fossil reptile skin shows that biological control on the distribution of endogenous organic components within fossilized soft tissue can be resolved. Mapped organic functional units within this approximately 50 Myr old specimen from the Green River Formation (USA) include amide and sulphur compounds. These compounds are most probably derived from the original beta keratin present in the skin because fossil leaf- and other non-skin-derived organic matter from the same geological formation do not show intense amide or thiol absorption bands. Maps and spectra from the fossil are directly comparable to extant reptile skin. Furthermore, infrared results are corroborated by several additional quantitative methods including Synchrotron Rapid Scanning X-Ray Fluorescence (SRS-XRF) and Pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS). All results combine to clearly show that the organic compound inventory of the fossil skin is different from the embedding sedimentary matrix and fossil plant material. A new taphonomic model involving ternary complexation between keratin-derived organic molecules, divalent trace metals and silicate surfaces is presented to explain the survival of the observed compounds. X-ray diffraction shows that suitable minerals for complex formation are present. Previously, this study would only have been possible with major destructive sampling. Non-destructive FTIR imaging methods are thus shown to be a valuable tool for understanding the taphonomy of high-fidelity preservation, and furthermore, may provide insight into the biochemistry of extinct organisms.
authors.affiliation: School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Manchester M13 9PL, UK.
authors.forename: N P
authors.initials: NP
authors.lastname: Edwards
authors.forename: H E
authors.initials: HE
authors.lastname: Barden
authors.forename: B E
authors.initials: BE
authors.lastname: van Dongen
authors.forename: P L
authors.initials: PL
authors.lastname: Manning
authors.forename: P L
authors.initials: PL
authors.lastname: Larson
authors.forename: U
authors.initials: U
authors.lastname: Bergmann
authors.forename: W I
authors.initials: WI
authors.lastname: Sellers
authors.forename: R A
authors.initials: RA
authors.lastname: Wogelius
mesh_terms: D000577:Amides / Q000032:analysis; D000818:Animals; D005580:Fossils; D008401:Gas Chromatography-Mass Spectrometry; D007259:Infrared Rays; D011309:Preservation, Biological; D012104:Reptiles / Q000033:anatomy & histology*; D012867:Skin / Q000737:chemistry* / Q000166:cytology*; D015206:Southwestern United States; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013457:Sulfur Compounds / Q000032:analysis; D017356:Synchrotrons
chemical_list: D000577:Amides; D013457:Sulfur Compounds