title: Magnetic levitating polymeric nano/microparticular substrates for three-dimensional tumor cell culture.
abstract: Herein, we describe magnetic cell levitation models using conventional polymeric microparticles or nanoparticles as a substrate for the three-dimensional tumor cell culture. When the magnetic force originating from the ring-shaped magnets overcame the gravitational force, the magnetic field-levitated KB tumor cells adhered to the surface area of magnetic iron oxide (Fe(3)O(4))-encapsulated nano/microparticles and concentrated clusters of levitated cells, ultimately developing tumor cells to tumor spheroids. These simple cell culture models may prove useful for the screening of anticancer drugs and their formulations.
authors.affiliation: Division of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743, Republic of Korea.
authors.forename: Woong Ryeol
authors.initials: WR
authors.lastname: Lee
authors.forename: Kyung Taek
authors.initials: KT
authors.lastname: Oh
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.forename: Na Young
authors.initials: NY
authors.lastname: Yoo
authors.forename: Yong Sik
authors.initials: YS
authors.lastname: Ahn
authors.forename: Don Haeng
authors.initials: DH
authors.lastname: Lee
authors.forename: Yu Seok
authors.initials: YS
authors.lastname: Youn
authors.forename: Deok-Keun
authors.initials: DK
authors.lastname: Lee
authors.forename: Kyung-Hoi
authors.initials: KH
authors.lastname: Cha
authors.forename: Eun Seong
authors.initials: ES
authors.lastname: Lee
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D011108:Polymers; C000499:ferric oxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid