title: Anti-angiogenic effects of the water extract of HangAmDan (WEHAD), a Korean traditional medicine.
abstract: We investigated the anti-angiogenic effects of the water extract of HangAmDan (WEHAD), which is a crude extract of nine Korean medicinal substances of animal and plant origin. In human umbilical vein endothelial cells, WEHAD significantly inhibited bFGF-induced proliferation, adhesion, migration, and capillary tube formation. We used an antibody array to perform an analysis of signaling proteins, which showed up-regulated expression of various proteins including RAD51, RAD52, and p73, and down-regulated expression of pFAK. Blood vessel formation in a chick chorioallantoic membrane (CAM) treated with WEHAD was markedly reduced in length compared with a PBS-treated control group. These results suggest that inhibition of angiogenesis by WEHAD may be the mechanism of action for the anti-cancer effects of HAD.
authors.affiliation: InnoPharmasceen Inc., Hoseo University, Asan, 336-795, Republic of Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Bang
authors.forename: Kyung-Soon
authors.initials: KS
authors.lastname: Kim
authors.forename: Eung-Yoon
authors.initials: EY
authors.lastname: Kim
authors.forename: Hwa-Seung
authors.initials: HS
authors.lastname: Yoo
authors.forename: Yeon-Weol
authors.initials: YW
authors.lastname: Lee
authors.forename: Chong-Kwan
authors.initials: CK
authors.lastname: Cho
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Choi
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Kang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D020869:Gene Expression Profiling; D006801:Humans; D055670:Medicine, Korean Traditional; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D056910:Republic of Korea; D015398:Signal Transduction / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology; D014867:Water / Q000737:chemistry*
chemical_list: D020533:Angiogenesis Inhibitors; D018797:Cell Cycle Proteins; D010936:Plant Extracts; D014867:Water; D016222:Fibroblast Growth Factor 2