title: Adaptive optics wide-field microscopy using direct wavefront sensing.
abstract: We report a technique for measuring and correcting the wavefront aberrations introduced by a biological sample using a Shack-Hartmann wavefront sensor, a fluorescent reference source, and a deformable mirror. The reference source and sample fluorescence are at different wavelengths to separate wavefront measurement and sample imaging. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved.
authors.affiliation: Jack Baskin School of Engineering, University of California, Santa Cruz, Santa Cruz, California 95064, USA. azucena@soe.ucsc.edu
authors.forename: Oscar
authors.initials: O
authors.lastname: Azucena
authors.forename: Justin
authors.initials: J
authors.lastname: Crest
authors.forename: Shaila
authors.initials: S
authors.lastname: Kotadia
authors.forename: William
authors.initials: W
authors.lastname: Sullivan
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Tao
authors.forename: Marc
authors.initials: M
authors.lastname: Reinig
authors.forename: Donald
authors.initials: D
authors.lastname: Gavel
authors.forename: Scot
authors.initials: S
authors.lastname: Olivier
authors.forename: Joel
authors.initials: J
authors.lastname: Kubby
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena