title: Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
abstract: BACKGROUND
  Mutations in several subunits of eukaryotic translation initiation factor 3 (eIF3) cause male transmission defects in Arabidopsis thaliana. To identify the stage of pollen development at which eIF3 becomes essential it is desirable to examine viable pollen and distinguish mutant from wild type. To accomplish this we have developed a broadly applicable method to track mutant alleles that are not already tagged by a visible marker gene through the male lineage of Arabidopsis.
  
  
  RESULTS
  Fluorescence tagged lines (FTLs) harbor a transgenic fluorescent protein gene (XFP) expressed by the pollen-specific LAT52 promoter at a defined chromosomal position. In the existing collection of FTLs there are enough XFP marker genes to track nearly every nuclear gene by virtue of its genetic linkage to a transgenic marker gene. Using FTLs in a quartet mutant, which yields mature pollen tetrads, we determined that the pollen transmission defect of the eif3h-1 allele is due to a combination of reduced pollen germination and reduced pollen tube elongation. We also detected reduced pollen germination for eif3e. However, neither eif3h nor eif3e, unlike other known gametophytic mutations, measurably disrupted the early stages of pollen maturation.
  
  
  CONCLUSIONS
  eIF3h and eIF3e both become essential during pollen germination, a stage of vigorous translation of newly transcribed mRNAs. These data delimit the end of the developmental window during which paternal rescue is still possible. Moreover, the FTL collection of mapped fluorescent protein transgenes represents an attractive resource for elucidating the pollen development phenotypes of any fine-mapped mutation in Arabidopsis.
authors.affiliation: Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, Knoxville, Tennessee, United States of America.
authors.forename: Bijoyita
authors.initials: B
authors.lastname: Roy
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Copenhaver
authors.forename: Albrecht G
authors.initials: AG
authors.lastname: von Arnim
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000378:metabolism*; D039621:Eukaryotic Initiation Factor-3 / Q000378:metabolism*; D005453:Fluorescence; D018525:Germination; D006579:Heterozygote; D008164:Luminescent Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics* / Q000254:growth & development*; D053205:Pollen Tube / Q000235:genetics / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; D039621:Eukaryotic Initiation Factor-3; D008164:Luminescent Proteins; D046912:Multiprotein Complexes