title: Cortical network from human embryonic stem cells.
abstract: The connection of embryonic stem cell technology and developmental biology provides valuable tools to decipher the mechanisms underlying human brain development and diseases, especially among neuronal populations, that are not readily available in primary cultures. It is obviously the case of neurons forming the human cerebral cortex. In the images that are presented, the neurons were generated in vitro from human embryonic stem cells via forebrain-like progenitors. Maintained in culture for prolonged time, they acquired a mainly glutamatergic phenotype and morphological characteristics of cortical pyramidal neurons, including dendritic spines, and formed spectacular networks.
authors.affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. Irina-Roxana.Nat@i-med.ac.at
authors.forename: Roxana
authors.initials: R
authors.lastname: Nat
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology; D049229:Dendritic Spines; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D005453:Fluorescence; D006801:Humans; D018613:Microscopy, Confocal; D009415:Nerve Net / Q000166:cytology* / Q000196:embryology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D016548:Prosencephalon / Q000166:cytology* / Q000196:embryology