title: Designed nucleotide binding motifs.
abstract: Gaps in the central strand of oligonucleotide triplexes bind nucleoside phosphates tightly. Watson-Crick and Hoogsteen base pairing as design principle yield motifs with high affinity for nucleoside phosphates with A or G as nucleobase, including ATP. The second messenger 3',5'-cAMP is bound with nanomolar affinity. A designed DNA motif accommodates seven nucleotides at a time. The design was implemented for both DNA and RNA.
authors.affiliation: Institute for Organic Chemistry, University of Stuttgart, 70569 Stuttgart, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kröner
authors.forename: Manuel
authors.initials: M
authors.lastname: Röthlingshöfer
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D003246:Conservation of Energy Resources / Q000379:methods; D000242:Cyclic AMP / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000737:chemistry / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D015290:Second Messenger Systems; D013816:Thermodynamics
chemical_list: D009711:Nucleotides; D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D000242:Cyclic AMP