title: Inter-species investigation of the mechano-regulation of bone healing: comparison of secondary bone healing in sheep and rat.
abstract: Inter-species differences in regeneration exist in various levels. One aspect is the dynamics of bone regeneration and healing, e.g. small animals show a faster healing response when compared to large animals. Mechanical as well as biological factors are known to play a key role in the process. However, it remains so far unknown whether different animals follow at all comparable mechano-biological rules during tissue regeneration, and in particular during bone healing. In this study, we investigated whether differences observed in vivo in the dynamics of bone healing between rat and sheep are only due to differences in the animal size or whether these animals have a different mechano-biological response during the healing process. Histological sections from in vivo experiments were compared to in silico predictions of a mechano-biological computer model for the simulation of bone healing. Investigations showed that the healing processes in both animal models occur under significantly different levels of mechanical stimuli within the callus region, which could explain histological observations of early intramembranous ossification at the endosteal side. A species-specific adaptation of a mechano-biological model allowed a qualitative match of model predictions with histological observations. Specifically, when keeping cell activity processes at the same rate, the amount of tissue straining defining favorable mechanical conditions for the formation of bone had to be increased in the large animal model, with respect to the small animal, to achieve a qualitative agreement of model predictions with histological data. These findings illustrate that geometrical (size) differences alone cannot explain the distinctions seen in the histological appearance of secondary bone healing in sheep and rat. It can be stated that significant differences in the mechano-biological regulation of the healing process exist between these species. Future investigations should aim towards understanding whether these differences are due to differences in cell behavior, material properties of the newly formed tissues within the callus and/or differences in response to the mechanical environment.
authors.affiliation: Julius Wolff Institute and Center for Musculoskeletal Surgery, Charité-Universitätsmedizin Berlin, Germany. sara.checa@charite.de
authors.forename: Sara
authors.initials: S
authors.lastname: Checa
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Prendergast
authors.forename: Georg N
authors.initials: GN
authors.lastname: Duda
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000502:physiology*; D049109:Cell Proliferation; D003198:Computer Simulation; D004548:Elasticity; D020342:Finite Element Analysis; D008954:Models, Biological; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D034561:Sheep, Domestic; D013045:Species Specificity; D013314:Stress, Mechanical