title: Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii.
abstract: The thermotolerant yeast strain isolated from sugarcane juice through enrichment technique was identified as a strain of Pichiakudriavzevii (Issatchenkiaorientalis) through molecular characterization. The P. kudriavzevii cells adapted to galactose medium produced about 30% more ethanol from sugarcane juice than the non-adapted cells. The recycled cells could be used for four successive cycles without a significant drop in ethanol production. Fermentation in a laboratory fermenter with galactose adapted P. kudriavzevii cells at 40°C resulted in an ethanol concentration and productivity of 71.9 g L(-1) and 4.0 g L(-1)h(-1), respectively from sugarcane juice composed of about 14% (w/v) sucrose, 2% (w/v) glucose and 1% (w/v) fructose. In addition to ethanol, 3.30 g L(-1) arabitol and 4.19 g L(-1) glycerol were also produced, whereas sorbitol and xylitol were not formed during fermentation. Use of galactose adapted P. kudriavzevii cells for ethanol production from sugarcane juice holds potential for scale-up studies.
authors.affiliation: Central Institute of Post Harvest Engineering and Technology, Ludhiana 141 004, India.
authors.forename: Sandeep Singh
authors.initials: SS
authors.lastname: Dhaliwal
authors.forename: Harinder Singh
authors.initials: HS
authors.lastname: Oberoi
authors.forename: Simranjeet Kaur
authors.initials: SK
authors.lastname: Sandhu
authors.forename: Dhiraj
authors.initials: D
authors.lastname: Nanda
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.forename: Satinder Kaur
authors.initials: SK
authors.lastname: Uppal
mesh_terms: D000222:Adaptation, Physiological; D000431:Ethanol / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D006358:Hot Temperature; D010802:Phylogeny; D010843:Pichia / Q000145:classification / Q000378:metabolism* / Q000502:physiology; D031786:Saccharum
chemical_list: D000431:Ethanol; D005690:Galactose