title: Development of andrographolide molecularly imprinted polymer for solid-phase extraction.
abstract: A method employing molecularly imprinted polymer (MIP) as selective sorbent for solid-phase extraction (SPE) to pretreat samples was developed. The polymers were prepared by precipitation polymerization with andrographolide as template molecule. The structure of MIP was characterized and its static adsorption capacity was measured by the Scatchard equation. In comparison with C(18)-SPE and non-imprinted polymer (NIP) SPE column, MIP-SPE column displays high selectivity and good affinity for andrographolide and dehydroandrographolide for extract of herb Andrographis paniculata (Burm.f.) Nees (APN). MIP-SPE column capacity was 11.9±0.6 μmol/g and 12.1±0.5 μmol/g for andrographolide and dehydroandrographolide, respectively and was 2-3 times higher than that of other two columns. The precision and accuracy of the method developed were satisfactory with recoveries between 96.4% and 103.8% (RSD 3.1-4.3%, n=5) and 96.0% and 104.2% (RSD 2.9-3.7%, n=5) for andrographolide and dehydroandrographolide, respectively. Various real samples were employed to confirm the feasibility of method. This developed method demonstrates the potential of molecularly imprinted solid phase extraction for rapid, selective, and effective sample pretreatment.
authors.affiliation: College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Wanli District, Nanchang, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Yin
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Liu
authors.forename: Yifan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yongming
authors.initials: Y
authors.lastname: Luo
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D012044:Regression Analysis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004224:Diterpenes; D011108:Polymers; D020106:Acrylamide; C030419:andrographolide; C478098:dehydroandrographolide