title: Microbial transformation of diosgenin by filamentous fungus Cunninghamella echinulata.
abstract: Microbial transformation of diosgenin (1) by suspended-cell cultures of the filamentous fungus Cunninghamella echinulata CGMCC 3.2000 was investigated. Incubation of the substrate diosgenin (1) with this fungus led to the isolation of three products: two known compounds, (25R)-spirost-5-en-3β,7β,12β-triol and (25R)-spirost-5-en-3β,7β,11α-triol, and a new compound (25R)-spirost-5-en-3β,7α,11α-triol. The structural elucidations of the three compounds were achieved mainly by the MS, 1D and 2D NMR spectroscopic methods and comparison with known compounds. C. echinulata CGMCC 3.2000 has not been used before in the biotransformation of diosgenin.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.forename: Xi-Kui
authors.initials: XK
authors.lastname: Liu
authors.forename: Shao-Bin
authors.initials: SB
authors.lastname: Fu
authors.forename: Di-An
authors.initials: DA
authors.lastname: Sun
mesh_terms: D001711:Biotransformation; D020093:Cunninghamella / Q000378:metabolism*; D004144:Diosgenin / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006900:Hydroxylation; D015394:Molecular Structure; D013237:Stereoisomerism
chemical_list: C558061:(25R)-spirost-5-en-3beta,7alpha,11alpha-triol; C558060:(25R)-spirost-5-en-3beta,7beta,12beta-triol; D004144:Diosgenin