title: [The delayed sensitization of CRH response developed after chronic variable stress on the acoustic startle reflex].
abstract: Substantial evidence indicates that brain neurons containing and secreting norepinephrine (NE) and corticotrophin-releasing hormone (CRH) are activated during stress. The acoustic startle reflex (ASR) can be enhanced by CRH neuronal activity in the central nucleus of the amygdala. Our previous study demonstrates an augmentation of the footshock-induced ASR (f-ASR) 1 day after chronic variable stress (CVS) for 13 days. In this study, to evaluate a long-term neural plasticity in NE-CRH systems after CVS, we examined f-ASR 1, 8 or 15 days after CVS. The augmented magnitude of the f-ASR 15 day after CVS was potentiated and delayed compared with that 1 day after CVS. The delayed augmentation of f-ASR was inhibited by repeated treatment with desipramine, maprotiline or paroxetine for 14 days after CVS. A single treatment with any antidepressant agent had no influence the f-ASR while a marked inhibition by a single dose of alprazolam, CRH1-receptor antagonist, prazosin and propranolol was observed. The decreased tyrosine hydroxylase activity in the locus coeruleus and the beta-adrenoceptor down-regulation in the amygdaloid complex might be involved in the inhibiton of the delayed augmentation of f-ASR by repeated antidepressant treatment, leading to the possibility that the delayed sensitization of CRH response to stress after CVS might contribute to the biological mechanism underlying the formation of pathological states such as anxiety and depressive disorders.
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, 216-8511 Japan. k2fujiwara@marianna-u.ac.jp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Mikio
authors.initials: M
authors.lastname: Asakura
authors.forename: Takuyou
authors.initials: T
authors.lastname: Yanagida
authors.forename: Miho
authors.initials: M
authors.lastname: Nakano
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kanai
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tanaka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sasuga
authors.forename: Kenichi
authors.initials: K
authors.lastname: Osada
mesh_terms: D000818:Animals; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D003891:Desipramine / Q000494:pharmacology; D008297:Male; D008376:Maprotiline / Q000494:pharmacology; D009638:Norepinephrine / Q000502:physiology; D017374:Paroxetine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018043:Receptors, Corticotropin / Q000037:antagonists & inhibitors; D012022:Reflex, Acoustic / Q000187:drug effects / Q000502:physiology*; D013216:Reflex, Startle / Q000187:drug effects / Q000502:physiology*; D013315:Stress, Psychological / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D018687:Antidepressive Agents, Second-Generation; D000929:Antidepressive Agents, Tricyclic; D018043:Receptors, Corticotropin; D008376:Maprotiline; D017374:Paroxetine; D003346:Corticotropin-Releasing Hormone; D003891:Desipramine; D009638:Norepinephrine