title: [Investigation on long-term survival of transplanted bone marrow mesenchymal stem cells in infarcted myocardium of rats].
abstract: OBJECTIVE
  To investigate the quantification and distribution of survival transplanted bone marrow mesenchymal stem cells (BMSCs) in infarcted myocardium of rats.
  
  
  METHODS
  BMSCs of male SD rats were isolated, purified and proliferated. Female SD rats were subjected to occlusion of left anterior descending artery, 1.2 × 10(6) BMSCs were injected into the centre zone of the infarct region 1 h after left anterior descending artery occlusion. Myocardial tissue was harvested randomly at 1, 3 and 6 (n = 18) weeks after transplantation. Transplanted cells were labeled with Hoechst33342 (n = 5) and Brdu (n = 5). Sry sequence of Y chromatosome in male rat was analyzed by real-time PCR in the tissue (n = 8).
  
  
  RESULTS
  FACS analysis showed that BMSCs expressed homogenous with CD44+/CD34-. Hoechst33342 or Brdu labeled positive cells were found in localized accumulation and some of them migrated to the margin of the infarct region, and vessel-like structure could be observed in the host hearts. The real-time PCR demonstrated that the survival rates of engrafted BMSCs were 7.88%, 7.82% and 8.73% respectively at 1, 3 and 6 weeks after transplantation.
  
  
  CONCLUSIONS
  BMSCs could survive in the host infarcted myocardium for more than one month and the survival rate was around 8% from the first week to the sixth week after transplantation.
authors.affiliation: Department of Cardiology, Second Military Medical University, Shanghai 200003, China.
authors.forename: Hong-min
authors.initials: HM
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Mei
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D005260:Female; D006085:Graft Survival; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley