title: The influenza A virus NS1 protein interacts with the nucleoprotein of viral ribonucleoprotein complexes.
abstract: The influenza A virus genome consists of eight RNA segments that associate with the viral polymerase proteins (PB1, PB2, and PA) and nucleoprotein (NP) to form ribonucleoprotein complexes (RNPs). The viral NS1 protein was previously shown to associate with these complexes, although it was not clear which RNP component mediated the interaction. Using individual TAP (tandem affinity purification)-tagged PB1, PB2, PA, and NP, we demonstrated that the NS1 protein interacts specifically with NP and not the polymerase subunits. The region of NS1 that binds NP was mapped to the RNA-binding domain.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.
authors.forename: Nicole C
authors.initials: NC
authors.lastname: Robb
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Chase
authors.forename: Katja
authors.initials: K
authors.lastname: Bier
authors.forename: Frank T
authors.initials: FT
authors.lastname: Vreede
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
authors.forename: Nadia
authors.initials: N
authors.lastname: Naffakh
authors.forename: Martin
authors.initials: M
authors.lastname: Schwemmle
authors.forename: Ervin
authors.initials: E
authors.lastname: Fodor
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D025941:Protein Interaction Mapping; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C085820:INS1 protein, influenza virus; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins