title: CD41 is developmentally regulated and differentially expressed on mouse hematopoietic stem cells.
abstract: CD41 expression is associated with the earliest stages of mouse hematopoiesis. It is notably expressed on some cells of the intra-aortic hematopoietic clusters, an area where the first adult-repopulating hematopoietic stem cells (HSCs) are generated. Although it is generally accepted that CD41 expression marks the onset of primitive/definitive hematopoiesis, there are few published data concerning its expression on HSCs. It is as yet uncertain whether HSCs express CD41 throughout development, and if so, to what level. We performed a complete in vivo transplantation analysis with yolk sac, aorta, placenta, and fetal liver cells, sorted based on CD41 expression level. Our data show that the earliest emerging HSCs in the aorta express CD41 in a time-dependent manner. In contrast, placenta and liver HSCs are CD41⁻. Thus, differential and temporal expression of CD41 by HSCs in the distinct hematopoietic territories suggests a developmental/dynamic regulation of this marker throughout development.
authors.affiliation: Department of Cell Biology, Erasmus Medical Center, Erasmus MC Stem Cell Institute, Rotterdam, The Netherlands.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robin
authors.forename: Katrin
authors.initials: K
authors.lastname: Ottersbach
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Boisset
authors.forename: Aneta
authors.initials: A
authors.lastname: Oziemlak
authors.forename: Elaine
authors.initials: E
authors.lastname: Dzierzak
mesh_terms: D000818:Animals; D001011:Aorta / Q000196:embryology / Q000378:metabolism; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008099:Liver / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010920:Placenta / Q000196:embryology / Q000378:metabolism; D040201:Platelet Membrane Glycoprotein IIb / Q000096:biosynthesis*; D011247:Pregnancy; D015017:Yolk Sac / Q000196:embryology / Q000378:metabolism
chemical_list: D040201:Platelet Membrane Glycoprotein IIb