title: Phytophthora infestans has a plethora of phospholipase D enzymes including a subclass that has extracellular activity.
abstract: In eukaryotes phospholipase D (PLD) is involved in many cellular processes. Currently little is known about PLDs in oomycetes. Here we report that the oomycete plant pathogen Phytophthora infestans has a large repertoire of PLDs divided over six subfamilies: PXPH-PLD, PXTM-PLD, TM-PLD, PLD-likes, and type A and B sPLD-likes. Since the latter have signal peptides we developed a method using metabolically labelled phospholipids to monitor if P. infestans secretes PLD. In extracellular medium of ten P. infestans strains PLD activity was detected as demonstrated by the production of phosphatidic acid and the PLD specific marker phosphatidylalcohol.
authors.affiliation: Laboratory of Phytopathology, Plant Sciences Group, Wageningen University, Wageningen, The Netherlands. Harold.Meijer@wur.nl
authors.forename: Harold J G
authors.initials: HJ
authors.lastname: Meijer
authors.forename: Hussen Harrun
authors.initials: HH
authors.lastname: Hassen
authors.forename: Francine
authors.initials: F
authors.lastname: Govers
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D005110:Extracellular Space / Q000201:enzymology*; D005796:Genes / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000469:parasitology; D010739:Phospholipase D / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D055750:Phytophthora infestans / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D010743:Phospholipids; D010739:Phospholipase D