title: In-vitro clot lytic potential of Fagonia arabica: a comparative study of two methods.
abstract: The tube method developed in our laboratory is a simple, inexpensive and a classical whole blood clot lytic procedure through which clot lytic potential of Fagonia arabica was found to be significant. Microtiter plate clot lysis (MPCL) assay is a rapid and precise turbidimetric clot lysis method which includes measurements of maximum absorbance (Max Abs), area under the curve (AUC) along with the standard clot lysis time. In the present study we have compared and validated clot lytic potential of F. arabica extract by tube method and MPCL assay. Percentage of clot lysis was calculated by measuring the difference of the absorbance taken at 0 and 240 min in the case of MPCL assay, whereas with the tube method according to the weight difference. Fagonia arabica (50 ug/ml) was capable of clot lysis by MPCL assay and showed clot lysis pattern similar to 60 U/ml streptokinase (positive control). The clot lysis times were significantly different from one another (P value ≤0.001). When Max Abs and AUC were compared to the clot lysis time the correlation coefficient (r value) was significant too (P value ≤0.001). Moreover, we have also found that both the methods showed almost the same clot lysis percentage by streptokinase as well as F. arabica. The correlation coefficient between streptokinase, and F. arabica done by tube method and MPCL assay was found to be statistically significant (P < 0.05). Fagonia arabica had the clot lytic potential checked by in-vitro methods, namely MPCL assay and the method.
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, 88/2 Bajaj Nagar, Nagpur, Maharashtra, India.
authors.forename: Sweta R
authors.initials: SR
authors.lastname: Chourasia
authors.forename: Rajpal Singh
authors.initials: RS
authors.lastname: Kashyap
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
authors.forename: Jayant Y
authors.initials: JY
authors.lastname: Deopujari
authors.forename: Girdhar M
authors.initials: GM
authors.lastname: Taori
authors.forename: Hatim F
authors.initials: HF
authors.lastname: Daginawala
mesh_terms: D019540:Area Under Curve; D001780:Blood Coagulation Tests; D005342:Fibrinolysis / Q000187:drug effects*; D009391:Nephelometry and Turbidimetry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D012995:Solubility; D013300:Streptokinase / Q000032:analysis / Q000378:metabolism; D028461:Zygophyllaceae / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013300:Streptokinase