title: Dietary fish oil and curcumin combine to modulate colonic cytokinetics and gene expression in dextran sodium sulphate-treated mice.
abstract: Both fish oil (FO) and curcumin have potential as anti-tumour and anti-inflammatory agents. To further explore their combined effects on dextran sodium sulphate (DSS)-induced colitis, C57BL/6 mice were randomised to four diets (2 × 2 design) differing in fatty acid content with or without curcumin supplementation (FO, FO+2 % curcumin, maize oil (control, MO) or MO+2 % curcumin). Mice were exposed to one or two cycles of DSS in the drinking-water to induce either acute or chronic intestinal inflammation, respectively. FO-fed mice exposed to the single-cycle DSS treatment exhibited the highest mortality (40 %, seventeen of forty-three) compared with MO with the lowest mortality (3 %, one of twenty-nine) (P = 0·0008). Addition of curcumin to MO increased (P = 0·003) mortality to 37 % compared with the control. Consistent with animal survival data, following the one- or two-cycle DSS treatment, both dietary FO and curcumin promoted mucosal injury/ulceration compared with MO. In contrast, compared with other diets, combined FO and curcumin feeding enhanced the resolution of chronic inflammation and suppressed (P < 0·05) a key inflammatory mediator, NF-κB, in the colon mucosa. Mucosal microarray analysis revealed that dietary FO, curcumin and FO plus curcumin combination differentially modulated the expression of genes induced by DSS treatment. These results suggest that dietary lipids and curcumin interact to regulate mucosal homeostasis and the resolution of chronic inflammation in the colon.
authors.affiliation: Program in Integrative Nutrition and Complex Diseases, Texas A&M University, College Station, TX, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jia
authors.forename: Ivan
authors.initials: I
authors.lastname: Ivanov
authors.forename: Zlatomir Z
authors.initials: ZZ
authors.lastname: Zlatev
authors.forename: Robert C
authors.initials: RC
authors.lastname: Alaniz
authors.forename: Brad R
authors.initials: BR
authors.lastname: Weeks
authors.forename: Evelyn S
authors.initials: ES
authors.lastname: Callaway
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Goldsby
authors.forename: Laurie A
authors.initials: LA
authors.lastname: Davidson
authors.forename: Yang-Yi
authors.initials: YY
authors.lastname: Fan
authors.forename: Lan
authors.initials: L
authors.lastname: Zhou
authors.forename: Joanne R
authors.initials: JR
authors.lastname: Lupton
authors.forename: David N
authors.initials: DN
authors.lastname: McMurray
authors.forename: Robert S
authors.initials: RS
authors.lastname: Chapkin
mesh_terms: D000208:Acute Disease; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects / Q000627:therapeutic use; D002908:Chronic Disease; D003092:Colitis / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D003474:Curcumin / Q000009:adverse effects / Q000627:therapeutic use*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D016264:Dextran Sulfate / Q000008:administration & dosage / Q000633:toxicity; D019587:Dietary Supplements; D005395:Fish Oils / Q000009:adverse effects / Q000627:therapeutic use*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007509:Irritants / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011897:Random Allocation; D016019:Survival Analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D005395:Fish Oils; D007509:Irritants; D016328:NF-kappa B; D016264:Dextran Sulfate; D003474:Curcumin