title: LNA for optimization of fluorescent oligonucleotide probes: improved spectral properties and target binding.
abstract: Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2'-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.
authors.affiliation: Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark , DK-5230 Odense M, Denmark.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Astakhova
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Ustinov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Korshun
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D008968:Molecular Conformation; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C477371:locked nucleic acid; D004247:DNA