title: Combinatorial optimization of multiple MALDI matrices on a single tissue sample using inkjet printing.
abstract: Taking advantage of the drop-on-demand capabilities of inkjet printing, the first example of a single tissue being used as a substrate for preparing combinatorial arrays of different matrix-assisted laser desorption/ionization (MALDI) matrices in multiple concentrations on a single chip is reported. By varying the number of droplets per spot that were printed, a gradient array of different amounts of matrix material could be printed on a single chip, while the selection of matrices could be adjusted by switching different matrix materials. The result was a two-dimensional array of multiple matrices on a single tissue slice, which could be analyzed microscopically and by MALDI to elucidate which combination of matrix and printing conditions offered the best resolution in terms of spot-to-spot distance and signal-to-noise ratios for proteins in the recorded MS spectra. This combinatorial approach enables the efficient optimization of possible matrices in an organized, side-by-side array format, which can particularly be useful for the detection of specific protein markers.
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry and Jena Center for Soft Matter, Friedrich Schiller University Jena , Humboldtstrasse 10, 07743 Jena, Germany.
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Delaney
authors.forename: Annett
authors.initials: A
authors.lastname: Urbanek
authors.forename: Liane
authors.initials: L
authors.lastname: Wehder
authors.forename: Jolke
authors.initials: J
authors.lastname: Perelaer
authors.forename: Anna C
authors.initials: AC
authors.lastname: Crecelius
authors.forename: Ferdinand
authors.initials: F
authors.lastname: von Eggeling
authors.forename: Ulrich S
authors.initials: US
authors.lastname: Schubert
mesh_terms: D000818:Animals; D020650:Combinatorial Chemistry Techniques; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet