title: [Alteration of PTEN gene expression affects the migration of human airway smooth muscle cells in vitro].
abstract: OBJECTIVE
  To investigate the changes in human airway smooth muscle cell (HASMC) migration and related signaling pathway after interference with PTEN gene expression.
  
  
  METHODS
  HASMCs were infected with an adenovirus vector and RNA interference vector of human PTEN gene to establish the cell model with PTEN gene over-expression (Ad-GFP-PTEN-HASMC) and one with PTEN gene silencing (Ad-shPTEN-HASMC), using Ad-GFP-infected and a blank cells as the negative controls and LY294002 as the positive control. Fluorescence microscopy and flow cytometric analysis were used to evaluate the transfection efficiency, and Western blotting was performed to examine the expression of PTEN and the activation of AKT and ERK1/2 signal pathway. Transwell assay and wound healing assay were used to assess the migration of HASMCs.
  
  
  RESULTS
  The adenovirus over-expression vector and RNA interference vector significantly affected the expression of human PTEN gene. Up-regulation of PTEN gene resulted in a slow-down of the HASMC migration, an inhibition of PI3K/AKT signal pathway at the protein level but no changes in Ras-Raf-MEK1/2-ERK1/2 signal pathway. Down-regulated PTEN gene expression, however, was not associated with an enhancement of HASMC migration, but activated PI3K/AKT signal pathway and inhibited Ras-Raf-MEK1/2-ERK1/2 signal pathway.
  
  
  CONCLUSIONS
  Upregulation of PTEN gene can effectively inhibit airway smooth muscle cell migration, the effect of which is probably mediated by the PI3K/AKT pathway.
authors.affiliation: Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. westlife_bsb@126.com
authors.forename: Yang
authors.initials: Y
authors.lastname: GAO
authors.forename: Hao-hai
authors.initials: HH
authors.lastname: ZHONG
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: LUO
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: LAI
authors.forename: Jian
authors.initials: J
authors.lastname: XU
authors.forename: Da-cheng
authors.initials: DC
authors.lastname: ZHANG
authors.forename: Hai-bing
authors.initials: HB
authors.lastname: Lan
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D001980:Bronchi / Q000166:cytology; D002465:Cell Movement; D002478:Cells, Cultured; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008168:Lung / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism* / Q000473:pathology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D034622:RNA Interference; D014162:Transfection
chemical_list: D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human