title: Effect of cigarette smoke components on vascular smooth muscle cell migration toward platelet-derived growth factor BB.
abstract: Cigarette smoking is one of the factors causing accumulation of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques. Changes in cell migration toward platelet-derived growth factor BB were investigated using a Boyden chamber after 48-h preincubation of GBaSM-4 VSMCs with nicotine or nicotine-free cigarette smoke extract (CSE). A nicotine concentration of 0.1 µM maximally promoted cell migration; 0.1% CSE also promoted cell migration, while high CSE concentrations damaged GBaSM-4 cells. Fetal bovine serum (FBS) long-depletion induced decrease in migration of GBaSM-4 cells. Our results suggest that nicotine and some CSE components can induce GBaSM-4 cell migration.
authors.affiliation: Department of Molecular and Cellular Pharmacology, Gunma University Graduate School of Medicine, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Yoshiyama
authors.forename: Takahiro
authors.initials: T
authors.lastname: Horinouchi
authors.forename: Soichi
authors.initials: S
authors.lastname: Miwa
authors.forename: Hong Hui
authors.initials: HH
authors.lastname: Wang
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kohama
authors.forename: Akio
authors.initials: A
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D000077214:Becaplermin; D002460:Cell Line; D054443:Cell Migration Assays / Q000379:methods; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D006168:Guinea Pigs; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology*; D009538:Nicotine / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects*
chemical_list: D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012906:Smoke; D000077214:Becaplermin; D009538:Nicotine