title: Plasminogen activator inhibitor type-1 gene 4G/5G polymorphism and polycystic ovary syndrome.
abstract: OBJECTIVE
  This study was conducted in Turkish patients with polycystic ovary syndrome (PCOS) to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene and to examine the role of this polymorphism in PCOS development.
  
  
  METHODS
  Genomic DNA obtained from 200 persons (100 patients with PCOS and 100 healthy controls) was used in the study. DNA was amplified by polymerase chain reaction using 4G and 5G allele-specific primers. Polymerase chain reaction products were assessed with charged-couple device camera after exposing to 2% agarose gel electrophoresis.
  
  
  RESULTS
  No statistically significant difference between the groups with respect to genotype distribution was found (p>0.05) in the study. The 4G and 5G allele frequencies were indicated as 51.5% and 48.5% in patients, respectively, whereas this was 51%-49% in the control group.
  
  
  CONCLUSIONS
  Consequently, it has been established by this study that the 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene do not play a role in the development of PCOS in the Turkish population.
authors.affiliation: Department of Obstetrics and Gynecology, Private Mus Sifa Hospital, Mus, Turkey.
authors.forename: Cetin
authors.initials: C
authors.lastname: Kilicci
authors.forename: Banu
authors.initials: B
authors.lastname: Bayram
authors.forename: Mete
authors.initials: M
authors.lastname: Ozkurt
authors.forename: Harun
authors.initials: H
authors.lastname: Onlu
authors.forename: Nilufer
authors.initials: N
authors.lastname: Erkasap
authors.forename: Fezan
authors.initials: F
authors.lastname: Mutlu
mesh_terms: D000328:Adult; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D011110:Polymorphism, Genetic; D014421:Turkey; D055815:Young Adult
chemical_list: D017395:Plasminogen Activator Inhibitor 1