title: LAS degradability by marine biofilms derived from seawater in Spain and Sweden.
abstract: Marine biofilms were established on glass beads with or without deliberate pre-exposure to LAS (20 μg/L) in Spain (Cadiz) and Sweden (Kristineberg). The ability of each community to mineralize LAS (100 μg/L) was then assessed in biometers at four experimental temperatures (between 6 and 21°C). Genetic diversity and biomass of the biofilms were assessed by genetic fingerprinting (DGGE) and direct bacterial counts. With biofilms from Sweden, where LAS was not detected in seawater (n = 3), deliberate pre-exposure to LAS resulted in lower genetic diversity and higher mineralization rate constant; however, with biofilms from Spain, where 6.4 ± 3.9 μgLAS/L (n = 3) was measured during the colonization, pre-exposure did not affect the bacterial community. Bacterial acclimation therefore appeared to have been induced at environmental concentrations < 6 μgLAS/L. Environmental pre-exposure was not a pre-requisite for featuring the full consortia of LAS degraders in the biometers. The mineralization rate was described using an Arrhenius equation at experimental temperatures within the typical annual range; however, they departed from this model below this range.
authors.affiliation: The Procter & Gamble Company, Brussels, Belgium; CSIC, Instituto de Ciencias Marinas de Andalucía, Puerto Real (Cádiz), Spain. aourell.mauffret@icman.csic.es
authors.forename: A
authors.initials: A
authors.lastname: Mauffret
authors.forename: D C
authors.initials: DC
authors.lastname: Gillan
authors.forename: K M
authors.initials: KM
authors.lastname: Eriksson
authors.forename: A
authors.initials: A
authors.lastname: Rottiers
authors.forename: J
authors.initials: J
authors.lastname: Blasco
authors.forename: A
authors.initials: A
authors.lastname: Temara
mesh_terms: D017738:Alkanesulfonic Acids / Q000032:analysis / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D018441:Biofilms; D018533:Biomass; D012623:Seawater / Q000737:chemistry / Q000382:microbiology*; D013030:Spain; D013501:Surface-Active Agents / Q000032:analysis / Q000378:metabolism; D013548:Sweden; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C030641:alkylbenzyl sulfonic acid