title: Primer extension reactions as a tool to uncover folding motifs within complex G-rich sequences: analysis of the human KRAS NHE.
abstract: We employed primer extension reactions to uncover folding motifs in a nuclease hypersensitive element (NHE) with a complex guanine pattern, located in the human KRAS promoter. We also identified and characterized a new G-rich motif of 21 nt capable of forming a parallel G-quadruplex that is disrupted by protein UP1.
authors.affiliation: Department of Medical and Biological Science, University of Udine, 33100 Udine, Italy.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Paramasivam
authors.forename: Susanna
authors.initials: S
authors.lastname: Cogoi
authors.forename: Luigi E
authors.initials: LE
authors.lastname: Xodo
mesh_terms: D020816:Amino Acid Motifs; D001665:Binding Sites; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D054856:G-Quadruplexes; D005786:Gene Expression Regulation; D011905:Genes, ras; D006147:Guanine / Q000032:analysis* / Q000378:metabolism; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000378:metabolism*; D006801:Humans; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017550:Spectroscopy, Fourier Transform Infrared; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; C000619656:hnRNPA1 protein, human; D006147:Guanine