title: A DNA biosensor based on graphene paste electrode modified with Prussian blue and chitosan.
abstract: A chemically modified graphene paste electrode was prepared by incorporating appropriate amounts of graphene in a paste mixture, followed by electrodepositing Prussian blue (PB) and coating chitosan on the electrode surface. The electrode was able to bind ssDNA, and gave a better voltammetric response for complement DNA than did ordinary carbon paste electrodes. The response of the electrode was characterized with respect to the paste composition, immobilization time of probe DNA on the chitosan and PB modified graphene paste electrode, and the effect of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC). The electrochemical behavior of PB assembled on the graphene paste electrode was investigated. The combination of graphene and PB can enhance the current response of the graphene paste electrode. As a consequence of DNA hybridization, a significant change in the current due to daunomycin intercalated with double-stranded DNA (ds-DNA) on the surface of the graphene paste electrode was observed.
authors.affiliation: Life and Environmental Science College, Shanghai Normal University, Shanghai, 200234, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bo
authors.forename: Weiqi
authors.initials: W
authors.lastname: Wang
authors.forename: Junfei
authors.initials: J
authors.lastname: Qi
authors.forename: Shasheng
authors.initials: S
authors.lastname: Huang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002244:Carbon / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D015342:DNA Probes; D004277:DNA, Single-Stranded / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005295:Ferrocyanides / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D012044:Regression Analysis
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D004800:Enzymes, Immobilized; D005295:Ferrocyanides; D002244:Carbon; D006108:Graphite; D048271:Chitosan; C000170:ferric ferrocyanide