title: Hydrogen peroxide decreases the survival rate of HeLa cells with stable knockdown of survival motor neuron protein.
abstract: The mutations of survival motor neuron (SMN) gene result in spinal muscular atrophy (SMA), a common neurodegenerative disease. Some of the motor neurons undergoing cell death is the predominant characteristic in SMA pathology. However, the viability and sensitivity to stresses of other cell types also need to be determined. In this article, we established HeLa stable cell line with inducible SMN knockdown to study its viability and sensitivity to oxidative stress. SMN knockdown in the HeLa stable cell line was induced by doxycycline. The proliferative and survival rates of SMN knockdown cells with or without hydrogen peroxide (H(2)O(2)) treatment were determined. Our results showed that the proliferative rate of SMN knockdown cells decreased only slightly compared with that of the cells without doxycycline treatment. In contrast, after H(2)O(2) reached certain concentrations, the survival rate of SMN knockdown cells decreased significantly. Our data indicate that SMN knockdown alone is not critical to cell viability. However, when SMN knockdown cells are under stress, such as oxidative stress, their survival rate may significantly decrease. Our results will be helpful to prevent the detrimental effect caused by the cell death of non-motor neurons under stress in SMA patients. In addition, the cell model we established can be used to study the mechanism and screen drugs to prevent the detrimental effects in cases of SMA disease.
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Ting-Yuan
authors.initials: TY
authors.lastname: Liu
authors.forename: Chung-Yee
authors.initials: CY
authors.lastname: Yuo
authors.forename: Cheng-Hsing
authors.initials: CH
authors.lastname: Kao
authors.forename: Chao-Neng
authors.initials: CN
authors.lastname: Tseng
authors.forename: Yuh-Jyh
authors.initials: YJ
authors.lastname: Jong
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
authors.forename: Shou-Mei
authors.initials: SM
authors.lastname: Wu
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D034741:RNA, Small Interfering; D055532:SMN Complex Proteins / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D055532:SMN Complex Proteins; D006861:Hydrogen Peroxide