title: B-cell transcription factors Pax-5, Oct-2, BOB.1, Bcl-6, and MUM1 are useful markers for the diagnosis of nodular lymphocyte predominant Hodgkin lymphoma.
abstract: In some instances, the overlap in morphologic features and antigen expression between nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) and classical Hodgkin lymphoma (cHL) can cause confusion in the diagnosis. In these cases, the transcription factors (TFs) B-cell specific activator protein (BSAP)/Pax-5, octamer binding protein-2 (Oct-2), B-lymphocyte-specific co-activator BOB.1/OBF.1, Bcl-6 protein and multiple myeloma-1/interferon regulatory factor-4 (MUM1/IRF-4) may aid in clarifying the diagnosis. Twenty-two cases of NLPHL were studied for the immunohistochemical expression of Pax-5, Oct-2, BOB.1, Bcl-6 protein and MUM1/IRF-4. Our results sustain the usefulness of the selected set of TFs to diagnose and distinguish NLPHL from cHL since Pax-5, Oct-2, BOB.1 and Bcl-6 are consistently expressed by lymphocyte predominant (LP) cells and reported by others to be often unexpressed in Hodgkin and Reed-Sternberg cells. By contrast, MUM1/IRF-4 protein scored negative in the majority of LP cells, but is reported to be expressed in almost all cases of cHL. Thus, although the expression of transcription factors is very heterogeneous, their simultaneous implementation for positive and differential diagnosis may be useful.
authors.affiliation: Department of Pathology, Emergency County Hospital, Timisoara, Romania. roseherbeck@yahoo.com
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Herbeck
authors.forename: D
authors.initials: D
authors.lastname: Teodorescu Brînzeu
authors.forename: Marioara
authors.initials: M
authors.lastname: Giubelan
authors.forename: Elena
authors.initials: E
authors.lastname: Lazăr
authors.forename: Alis
authors.initials: A
authors.lastname: Dema
authors.forename: Hortensia
authors.initials: H
authors.lastname: Ioniţă
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006689:Hodgkin Disease / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D050835:Interferon Regulatory Factors / Q000378:metabolism*; D050813:Octamer Transcription Factor-2 / Q000378:metabolism*; D051757:PAX5 Transcription Factor / Q000378:metabolism*; D051560:Proto-Oncogene Proteins c-bcl-6; D015534:Trans-Activators / Q000378:metabolism*; D000099218:Interferon Regulatory Factor-4
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D050835:Interferon Regulatory Factors; D050813:Octamer Transcription Factor-2; D051757:PAX5 Transcription Factor; D051560:Proto-Oncogene Proteins c-bcl-6; D015534:Trans-Activators; D000099218:Interferon Regulatory Factor-4; C084103:BCL6 protein, human; C497281:PAX5 protein, human; C091546:POU2AF1 protein, human; C058435:POU2F2 protein, human