title: Anti-triatomine saliva immunoassays for the evaluation of impregnated netting trials against Chagas disease transmission.
abstract: Insecticide-impregnated nets can kill triatomine bugs, but it remains unclear whether they can protect against Chagas disease transmission. In a field trial in Quequeña, Peru, sentinel guinea pigs placed in intervention enclosures covered by deltamethrin-treated nets showed significantly lower antibody responses to saliva of Triatoma infestans compared with animals placed in pre-existing control enclosures. Our results strongly suggest that insecticide-treated nets prevent triatomine bites and can thereby protect against infection with Trypanosoma cruzi. Anti-salivary immunoassays are powerful new tools to evaluate intervention strategies against Chagas disease.
authors.affiliation: Laboratory of Genomics and Proteomics of Disease Vectors, Institute of Parasitology, Biology Centre of the Academy of Sciences of Czech Republic, České Budĕjovice, Czech Republic.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schwarz
authors.forename: Jenny Ancca
authors.initials: JA
authors.lastname: Juarez
authors.forename: Jean
authors.initials: J
authors.lastname: Richards
authors.forename: Bruno
authors.initials: B
authors.lastname: Rath
authors.forename: Victor Quispe
authors.initials: VQ
authors.lastname: Machaca
authors.forename: Yagahira E
authors.initials: YE
authors.lastname: Castro
authors.forename: Edith S
authors.initials: ES
authors.lastname: Málaga
authors.forename: Katelyn
authors.initials: K
authors.lastname: Levy
authors.forename: Robert H
authors.initials: RH
authors.lastname: Gilman
authors.forename: Caryn
authors.initials: C
authors.lastname: Bern
authors.forename: Manuela
authors.initials: M
authors.lastname: Verastegui
authors.forename: Michael Z
authors.initials: MZ
authors.lastname: Levy
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000517:prevention & control*; D005260:Female; D006168:Guinea Pigs; D007118:Immunoassay; D007299:Insect Bites and Stings / Q000276:immunology / Q000517:prevention & control*; D007300:Insect Control / Q000379:methods*; D057148:Insecticide-Treated Bednets; D008297:Male; D010274:Parasitology / Q000379:methods; D010568:Peru; D012463:Saliva / Q000276:immunology*; D014224:Triatoma / Q000276:immunology*