title: [Significance of chemokine receptor CXCR3 expression in breast cancer].
abstract: OBJECTIVE
  To investigate the expression of CXCR3 and its association with clinicopathologic features in breast carcinoma.
  
  
  METHODS
  The expression level of CXCR3 in 18 samples of breast cancer and corresponding normal tissues was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR analysis. Immunohistochemistry was carried out to examine the expression of CXCR3 in 80 breast cancers, 20 fibroadenomas and 15 normal breast tissues.
  
  
  RESULTS
  (1) RT-PCR and real-time RT-PCR analysis showed a higher level of CXCR3 in breast cancer tissues than that in the corresponding normal breast tissues (P < 0.05). (2) Immunohistochemistry analysis showed that the positive rate of CXCR3 in breast cancer tissues was significantly higher than that in fibroadenomas and the normal breast tissues (P < 0.05). The expression level of CXCR3 in the lymph node-positive group was higher than that in the lymph node-negative group (P < 0.05). The expression of CXCR3 was positively correlated with the number of lymph nodes involved by metastasis, tumor size and pTNM tumor stage (P < 0.05).
  
  
  CONCLUSIONS
  Chemokine receptor CXCR3 was up-regulated in breast cancer, and was associated with the progression of breast cancer. CXCR3 might be a novel molecular marker to predict lymph node metastasis and prognosis of breast cancer.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Lu
authors.forename: Shuang-ni
authors.initials: SN
authors.lastname: Yu
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Luo
authors.forename: Wu-gan
authors.initials: WG
authors.lastname: Zhao
authors.forename: Yi-hui
authors.initials: YH
authors.lastname: Ma
authors.forename: Cong-wei
authors.initials: CW
authors.lastname: Jia
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D018226:Fibroadenoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D054367:Receptors, CXCR3 / Q000235:genetics / Q000378:metabolism*; D047368:Tumor Burden
chemical_list: D014408:Biomarkers, Tumor; C515187:CXCR3 protein, human; D012333:RNA, Messenger; D054367:Receptors, CXCR3