title: [Molecular cloning and protein structure prediction of barley (Hordeum vulgare L.) Dhn6 gene and its expression pattern under dehydration conditions].
abstract: Dehydrins (DHNs), proteins with protective functions encoded by the late embryogenesis abundant (LEA), are differentially up-regulated at the transcriptional level under environmental stresses such as water deficit, salinity, and low temperature or in response to ABA. Data involving protein structure and expression of dehydrins could elucidate their functional roles under dehydration conditions in plants. Dhn6 gene of 1767 bp with an intron (92 bp) in six-rowed barley, a member of LEA D11, was cloned into pMD18-T vector in the present study. It shared 93.18% identity with Hordeum vulgare ssp. vulgare (GenBank accession: AF043091), encoding a protein composed of 523 amino acid residues with the predicted molecular weight of 49.68 kDa and the theoretical isoelectric point of 8.04. Analyses of domain and structure indicated that the protein was dominantly composed of 83% hydrophillic amino acid residues, with numerous imperative curls and three loosed helixes. Three-dimensional measurement revealed that the water-soluble lipid-associating protein was attributed to its twisted cable formed by reverse paralleled chains. Moreover, the putative amphipathic α-helices formed by K-segments might play roles in protecting membrane structure in barley. Significantly, relatively high accumulations of Dhn6 gene were detected after 8 h of water deficit by real-time quantitative RT-PCR. These results possibly suggested that the accumulation of Dhn6 gene could be critical for resistant dehydration in plants.
authors.affiliation: Department of Cell Biology and Genetics, Zunyi Medical College, and Department of Interventional Radiology, the Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China. pengjiaqiong@163.com
authors.forename: Gang
authors.initials: G
authors.lastname: Qian
authors.forename: Jun-Jiao
authors.initials: JJ
authors.lastname: Ping
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Su-Yuan
authors.initials: SY
authors.lastname: Luo
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Li
authors.forename: Ming-Zhen
authors.initials: MZ
authors.lastname: Yang
authors.forename: Da
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001947:Breeding; D003001:Cloning, Molecular; D019295:Computational Biology; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D001467:Hordeum / Q000235:genetics* / Q000502:physiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant