title: CCR6hiCD11c(int) B cells promote M-cell differentiation in Peyer's patch.
abstract: M cells are responsible for uptake of mucosal antigens in Peyer's patches (PPs). Differentiation of M cells is thought to be induced by interactions between follicle-associated epithelium and PP cells; however, it remains elusive what types of immune cells function as M-cell inducers. Here, we attempted to identify the cells that serve as an M-cell inducer in PP. We found that a unique B-cell subset characterized by CCR6(hi)CD11c(int) resided in the subepithelial dome (SED) in mouse PP. CCR6(hi)CD11c(int) B cells showed chemotactic migration in response to CCL20. Furthermore, this unique B-cell subset substantially decreased in PP of CCR6-deficient mice, indicating that the SED localization of CCR6(hi)CD11c(int) B cells is most likely regulated by the CCL20-CCR6 system. Concomitantly, CCR6 deficiency caused remarkable decrement of M cells. Moreover, adoptive transfer of CCR6(hi)CD11c(int) B cells from wild-type mice restored the M-cell decrement in CCR6-deficient mice. Collectively, the spatial regulation of CCR6(hi)CD11c(int) B cells via the CCL20-CCR6 system may play a vital role in M-cell differentiation in mice.
authors.affiliation: Laboratory for Epithelial Immunobiology, Research Center for Allergy and Immunology, RIKEN, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ebisawa
authors.forename: Koji
authors.initials: K
authors.lastname: Hase
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitamura
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Knoop
authors.forename: Ifor R
authors.initials: IR
authors.lastname: Williams
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohno
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D039521:CD11c Antigen / Q000096:biosynthesis; D002454:Cell Differentiation; D002465:Cell Movement / Q000235:genetics; D002478:Cells, Cultured; D054418:Chemokine CCL20 / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010581:Peyer's Patches / Q000166:cytology*; D054399:Receptors, CCR6 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: C106311:CCR6 protein, human; D039521:CD11c Antigen; D054418:Chemokine CCL20; D054399:Receptors, CCR6