title: Comprehensive two-dimensional liquid chromatography with evaporative light-scattering detection for the analysis of triacylglycerols in Borago officinalis.
abstract: An optimized 2-D liquid chromatography (LC×LC) set-up, based on the different selectivities of a silver ion (Ag) and a non-aqueous reversed phase (NARP), employed in the first (D1) and the second dimension (D2), respectively, in combination with evaporative light-scattering detection (ELSD), has been developed for the analysis of the triacylglycerol (TAG) fraction in a Borago officinalis oil. The 2-D set-up, thanks to the complementary separation selectivity provided by the two columns, allowed to distribute 78 TAGs throughout the 2-D LC retention plane otherwise unachievable by 1-D LC.
authors.affiliation: Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Messina, Italy. lmondello@unime.it
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
authors.forename: Marco
authors.initials: M
authors.lastname: Beccaria
authors.forename: Paola
authors.initials: P
authors.lastname: Donato
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dugo
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
mesh_terms: D029683:Borago / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D014280:Triglycerides