title: Mediterranean diet supplemented with coenzyme Q10 induces postprandial changes in p53 in response to oxidative DNA damage in elderly subjects.
abstract: Coenzyme Q10 (CoQ) is a powerful antioxidant that reduces oxidative stress. We explored whether the quality of dietary fat alters postprandial oxidative DNA damage and whether supplementation with CoQ improves antioxidant capacity by modifying the activation/stabilization of p53 in elderly subjects. In this crossover study, 20 subjects were randomly assigned to receive three isocaloric diets during 4 weeks each: (1) Mediterranean diet (Med diet), (2) Mediterranean diet supplemented with CoQ (Med+CoQ diet), and (3) saturated fatty acid-rich diet (SFA diet). Levels of mRNAs were determined for p53, p21, p53R2, and mdm2. Protein levels of p53, phosphorylated p53 (Ser20), and monoubiquitinated p53 were also measured, both in cytoplasm and nucleus. The extent of DNA damage was measured as plasma 8-OHdG. SFA diet displayed higher postprandial 8-OHdG concentrations, p53 mRNA and monoubiquitinated p53, and lower postprandial Mdm2 mRNA levels compared with Med and Med+CoQ diets (p < 0.05). Moreover, Med+CoQ diet induced a postprandial decrease of cytoplasmatic p53, nuclear p-p53 (Ser20), and nuclear and cytoplasmatic monoubiquitinated p53 protein (p < 0.05). In conclusion, Med+CoQ diet improves oxidative DNA damage in elderly subjects and reduces processes of cellular oxidation. Our results suggest a starting point for the prevention of oxidative processes associated with aging.
authors.affiliation: Lipid and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Cordoba, Spain.
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Gutierrez-Mariscal
authors.forename: Pablo
authors.initials: P
authors.lastname: Perez-Martinez
authors.forename: Javier
authors.initials: J
authors.lastname: Delgado-Lista
authors.forename: Elena M
authors.initials: EM
authors.lastname: Yubero-Serrano
authors.forename: Antonio
authors.initials: A
authors.lastname: Camargo
authors.forename: Nieves
authors.initials: N
authors.lastname: Delgado-Casado
authors.forename: Cristina
authors.initials: C
authors.lastname: Cruz-Teno
authors.forename: Monica
authors.initials: M
authors.lastname: Santos-Gonzalez
authors.forename: Fernando
authors.initials: F
authors.lastname: Rodriguez-Cantalejo
authors.forename: Justo P
authors.initials: JP
authors.lastname: Castaño
authors.forename: Jose M
authors.initials: JM
authors.lastname: Villalba-Montoro
authors.forename: Francisco
authors.initials: F
authors.lastname: Fuentes
authors.forename: Francisco
authors.initials: F
authors.lastname: Perez-Jimenez
authors.forename: Jose
authors.initials: J
authors.lastname: Lopez-Miranda
mesh_terms: D000368:Aged; D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D015153:Blotting, Western; D018592:Cross-Over Studies; D004249:DNA Damage / Q000187:drug effects*; D038441:Diet, Mediterranean; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005260:Female; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D016158:Genes, p53 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D019518:Postprandial Period / Q000187:drug effects* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D014815:Vitamins / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D014815:Vitamins; D014451:Ubiquinone; C024989:coenzyme Q10