title: Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction.
abstract: A novel method for determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis coupled with online electrochemiluminescence detection with ultrasonic-assisted extraction has been developed. The effects of several factors such as detection potential, concentration and pH of phosphate buffer, separation voltage, injection time, ultrasonic power and extraction time were investigated. Under optimal conditions, the linear concentration range for pseudolycorine was 0.002-2 μg/mL with a correlation coefficient of 0.9991. Relative standard deviations of migration time and peak areas were 1.4 and 3.2%, respectively. The limit of detection (S/N=3) was 0.46 ng/mL. The proposed method can be successfully applied to the determination of pseudolycorine in the bulb of lycoris radiata.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, PR China. dengby16@163.com
authors.forename: Biyang
authors.initials: B
authors.lastname: Deng
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.forename: Huihui
authors.initials: H
authors.lastname: Yin
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Shangying
authors.initials: S
authors.lastname: Hu
authors.forename: Baofeng
authors.initials: B
authors.lastname: Li
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D005591:Chemical Fractionation; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008163:Luminescent Measurements; D031426:Lycoris / Q000737:chemistry*; D010617:Phenanthridines / Q000032:analysis* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013010:Sonication / Q000379:methods*
chemical_list: D047151:Amaryllidaceae Alkaloids; D010617:Phenanthridines; C027861:pseudolycorine