title: Development of a recombinant CHO cell model for the investigation of CAR and DAF role during early steps of echovirus 6 infection.
abstract: The early steps of echovirus 6 (E6) infection remain poorly understood and the only described receptor for haemagglutinating E6 strains is the decay accelerating factor (DAF). There is, however, accumulating evidence suggesting that E6 interaction with DAF is necessary but not sufficient for infection. In this report, we investigated the role of the coxsackie-adenovirus-receptor (CAR) as a potential DAF co-receptor during E6 infection. Using stably transfected Chinese Hamster Ovary (CHO) cells expressing CAR and DAF receptors, we found that DAF expression allowed attachment of both haemagglutinating and non-haemagglutinating E6 strains but was not sufficient for promoting E6 cell entry. Interestingly, the co-expression of DAF and CAR rendered 0.1-0.2% of cells permissive to some E6 strains' infection. Although our results did not show a major role of the CAR/DAF cooperation for E6 infection, it nevertheless indicated the use of CAR in the cell entry step of some minor E6 quasispecies. Moreover, the present report validates the use of recombinant CHO cells as valuable cellular model for the further characterisation of E6 receptors.
authors.affiliation: Unité de Virologie Médicale et Moléculaire, Centre Hospitalier Universitaire de Reims, Reims, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Renois
authors.forename: Saw-See
authors.initials: SS
authors.lastname: Hong
authors.forename: Richard
authors.initials: R
authors.lastname: Le Naour
authors.forename: Valérie
authors.initials: V
authors.lastname: Gafa
authors.forename: Déborah
authors.initials: D
authors.lastname: Talmud
authors.forename: Laurent
authors.initials: L
authors.lastname: Andréoletti
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lévêque
mesh_terms: D000818:Animals; D018958:CD55 Antigens / Q000378:metabolism*; D016466:CHO Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D003412:Cricetulus; D004458:Echovirus 6, Human / Q000502:physiology*; D006801:Humans; D011991:Receptors, Virus / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D018958:CD55 Antigens; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus