title: Bcl-XL prevents serum deprivation-induced oxidative stress mediated by Romo1.
abstract: B-cell lymphoma-extra large (Bcl-XL) has been known to suppress serum deprivation-induced cell death, while reactive oxygen species modulator 1 (Romo1) is responsible for a serum deprivation-induced increase in reactive oxygen species (ROS). Therefore, we investigated whether Bcl-XL expression could inhibit the serum deprivation-induced increase in ROS and cell death, which are mediated by Romo1. We found that Bcl-XL expression effectively blocked serum deprivation- and Romo1-triggered ROS generation. Bcl-XL also inhibited apoptotic cell death induced by both serum deprivation and oxidative stress. From these results, we suggest that increased Bcl-XL expression, which is observed in many cancer cells, confers resistance to oxidative stress in the cancer cells by suppressing Romo1-mediated oxidative stress.
authors.affiliation: Laboratory of Molecular Cell Biology, Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: Seung Baek
authors.initials: SB
authors.lastname: Lee
authors.forename: Hyung Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jungar
authors.initials: J
authors.lastname: Shin
authors.forename: Sung Tae
authors.initials: ST
authors.lastname: Kang
authors.forename: Seongman
authors.initials: S
authors.lastname: Kang
authors.forename: Young Do
authors.initials: YD
authors.lastname: Yoo
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002460:Cell Line; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D044967:Serum / Q000378:metabolism*; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D024101:Mitochondrial Proteins; C513338:ROMO1 protein, human; D017382:Reactive Oxygen Species; D051020:bcl-X Protein