title: Effect of dietary vanadium on cecal tonsil T cell subsets and IL-2 contents in broilers.
abstract: The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on intestinal immune function by histopathological observation of cecal tonsil and changes of the cecal tonsil T cell subsets by method of flow cytometry. Four hundred twenty 1-day-old avian broilers were divided into six groups and fed on a corn-soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and 60 mg/kg vanadium supplied as ammonium metavanadate. In comparison with those of control group, lymphocytes in the lymphatic nodule of cecal tonsil were apparently decreased in 45 and 60 mg/kg groups. The percentage of CD(3)(+) T cells was decreased (p < 0.05) in 45 mg/kg group at 28 and 42 days of age and significantly decreased (p < 0.01) in 60 mg/kg group at 28 and 42 days of age. The percentages of CD(3)(+)CD(4)(+) and CD(3)(+)CD(8)(+) T cells were markedly decreased (p < 0.05 or p < 0.01) in 60 mg/kg group from 14 to 28 days of age and were decreased (p < 0.05) in 45 mg/kg group at 28 and 42 days of age. However, changes of the CD(4)(+)/CD(8)(+) ratio were not significant. Meanwhile, the cecal tonsil interleukin-2 (IL-2) contents were decreased (p < 0.05 or p < 0.01) in 45 and 60 mg/kg groups from 14 to 42 days of age. It was concluded that dietary vanadium in excess of 30 mg/kg reduced the percentages of cecal tonsil T cells subsets and IL-2 contents, and caused cecal tonsil lesions, which impaired cecal tonsil function and impacted the local mucosal immune function of the intestines in broilers.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
authors.forename: Yuanxin
authors.initials: Y
authors.lastname: Deng
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Kangping
authors.initials: K
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
mesh_terms: D000375:Aging / Q000502:physiology; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D016516:CD4-CD8 Ratio; D002432:Cecum / Q000166:cytology* / Q000187:drug effects / Q000473:pathology; D002645:Chickens / Q000502:physiology*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D018928:Immunity, Mucosal / Q000187:drug effects; D007376:Interleukin-2 / Q000096:biosynthesis*; D018655:Lymphocyte Count; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000473:pathology; D014639:Vanadium / Q000494:pharmacology*
chemical_list: D007376:Interleukin-2; D014639:Vanadium