title: Isolation of Mycobacterium ulcerans from swab and fine-needle-aspiration specimens.
abstract: For cultivation of Mycobacterium ulcerans from clinical specimens, we optimized the release of bacteria from swabs, as well as decontamination and cultivation on supplemented medium. Nevertheless, the proportions of positive cultures, 41.7% (5/12) for fine-needle-aspiration (FNA) samples and 43.8% (49/112) for swab samples, were lower than those we have previously observed for excised tissue specimens.
authors.affiliation: Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana. dyeboah-manu@noguchi.mimcom.org
authors.forename: Dorothy
authors.initials: D
authors.lastname: Yeboah-Manu
authors.forename: Emelia
authors.initials: E
authors.lastname: Danso
authors.forename: Kobina
authors.initials: K
authors.lastname: Ampah
authors.forename: Adwoa
authors.initials: A
authors.lastname: Asante-Poku
authors.forename: Zuliehatu
authors.initials: Z
authors.lastname: Nakobu
authors.forename: Gerd
authors.initials: G
authors.lastname: Pluschke
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D044963:Biopsy, Fine-Needle / Q000379:methods; D054312:Buruli Ulcer / Q000175:diagnosis* / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D019911:Mycobacterium ulcerans / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
chemical_list: D003470:Culture Media