title: Proteomic analysis of changes in protein expression in liver mitochondria in apoE knockout mice.
abstract: The involvement of both apolipoprotein E (apoE) and mitochondria in lipid metabolism is widely recognized, however there is surprisingly scarce data about the putative mitochondrial action(s) of this protein. The aim of the study was to screen the alterations in liver mitochondrial proteome caused by apoE deficiency. We applied 2DE-LC-MS/MS methodology to investigate the changes in liver mitochondrial protein expression in 6-months old apoE(-/-) mice as compared to C57BL/6J controls. ApoE(-/-), but not C57BL/6J mice developed visible atherosclerotic changes in aorta and mild, diffuse steatosis of the liver. Collectively, 18 differentially expressed proteins were identified in mitochondria, related to apoptosis, antioxidant and detoxifying mechanisms of mitochondria, as well as lipid metabolism and transport. In conclusion, differential proteomic approach revealed several lines of proteomic evidence that mitochondrial function in the liver of apoE(-/-) mice could be altered as a result of overlapping of pathological and compensatory changes in expression of proteins.
authors.affiliation: Chair of Pharmacology, Jagiellonian University Medical College, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Suski
authors.forename: Rafał
authors.initials: R
authors.lastname: Olszanecki
authors.forename: Józef
authors.initials: J
authors.lastname: Madej
authors.forename: Justyna
authors.initials: J
authors.lastname: Totoń-Żurańska
authors.forename: Anna
authors.initials: A
authors.lastname: Niepsuj
authors.forename: Jacek
authors.initials: J
authors.lastname: Jawień
authors.forename: Beata
authors.initials: B
authors.lastname: Bujak-Giżycka
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Okoń
authors.forename: Ryszard
authors.initials: R
authors.lastname: Korbut
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008930:Mitochondria, Liver / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods
chemical_list: D001057:Apolipoproteins E; D020543:Proteome