title: Repressor of GATA negatively regulates murine contact hypersensitivity through the inhibition of type-2 allergic responses.
abstract: Repressor of GATA (ROG) inhibits Th2 cell differentiation and allergic airway inflammation in the lung. To determine the role of ROG in the pathogenesis of contact hypersensitivity (CHS), a hapten-induced mouse model of CHS using ROG Tg and ROG-deficient (ROG(-/-)) was used. ROG Tg mice showed little ear swelling, while ROG(-/-) mice showed enhanced ear swelling in comparison to wild type mice. Interstitial edema and mast cell degranulation at the local inflammation sites were mild in ROG Tg mice and exacerbated in ROG(-/-) mice. In addition, the serum total IgE and hapten-specific IgG1 levels were increased in ROG(-/-) mice. Adoptive transfer of ROG(-/-) CD4(+) T cells exacerbated CHS in wild type mice, while transfer of ROG Tg CD4(+) T cells resulted in the attenuation of CHS. These results indicate ROG negatively regulates the induction of CHS by controlling the CD4(+) T cell-mediated allergic responses, including IgE generation and mast cell degranulation.
authors.affiliation: Department of Immunology, Graduate School of Medicine, Chiba University, Inohana, Chuo-ku, Chiba, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Hirasaki
authors.forename: Chiaki
authors.initials: C
authors.lastname: Iwamura
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Yamashita
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ito
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kitajima
authors.forename: Kenta
authors.initials: K
authors.lastname: Shinoda
authors.forename: Takao
authors.initials: T
authors.lastname: Namiki
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Terasawa
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D004195:Disease Models, Animal; D006651:Histocytochemistry; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012097:Repressor Proteins / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D012097:Repressor Proteins; C406863:Rog protein, mouse; D007073:Immunoglobulin E