title: Effect of quercetin on formation of choroidal neovascularization (CNV) in age-related macular degeneration(AMD).
abstract: OBJECTIVE
  Age-related macular degeneration (AMD) as a disease entity is "dry" at early stage and made up of two main components at late stage: atrophic AMD and exudative AMD. Quercetin acts as an anti-oxidant to protect retinal pigment epithelial cells (RPE) from damaged by oxidative stress, but its effect on formation of choroidal neovascularization (CNV) in AMD is unclear. The aim of this study is to investigate the effect of quercetin on the formation of CNV in AMD.
  
  
  METHODS
  The development of CNV induced by laser was detected by fluorescein angiography (FA). Colored microsphere technique was used to determine the choroidal blood flow in ocular hypertensive rabbit eyes. In in vitro studies, HUVECs were treated with NaIO3, H2O2 and NaN3 to induce oxidative cell damages. The effect of quercetin on various oxidations-induced injuries in HUVECs was measured by MTT assay. HUVECs migration was assessed using a wound healing assay.
  
  
  RESULTS
  Quercetin significantly inhibited the formation of laser-induced CNV. The choroidal blood flow in rabbit eyes was significantly increased after quercetin instillation. In vitro results showed quercetin enhanced various oxidations-induced injuries in HUVECs and inhibited migration of HUVECs during wound healing.
  
  
  CONCLUSIONS
  Quercetin inhibited the formation of CNV both in vivo and in vitro and increased choroidal blood flow. It could become a promising candidate for the treatment of AMD.
authors.affiliation: Institute of Ocular Pharmacology, Texas A&M Health Science Center, College Station, TX 77843, USA.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhuang
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.forename: Bao Qin
authors.initials: BQ
authors.lastname: Lin
authors.forename: Wan Yu
authors.initials: WY
authors.lastname: Zhang
authors.forename: George C Y
authors.initials: GC
authors.lastname: Chiou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002829:Choroid / Q000098:blood supply / Q000187:drug effects; D020256:Choroidal Neovascularization / Q000209:etiology / Q000517:prevention & control*; D005451:Fluorescein Angiography; D061307:Human Umbilical Vein Endothelial Cells; D006861:Hydrogen Peroxide; D007452:Iodates; D007834:Lasers; D008268:Macular Degeneration / Q000150:complications*; D011794:Quercetin / Q000494:pharmacology*; D011817:Rabbits; D019810:Sodium Azide; D057135:Wet Macular Degeneration
chemical_list: D000975:Antioxidants; D007452:Iodates; D019810:Sodium Azide; D011794:Quercetin; D006861:Hydrogen Peroxide; C032285:sodium iodate