title: Sustained photo-hydrogen production by Chlorella pyrenoidosa without sulfur depletion.
abstract: The sustained production of H(2) by Chlorella pyrenoidosa was achieved without sulfur deficiency or PSII inhibition. C. pyrenoidosa preserved hydrogenase activity for several hours in the dark. Hydrogenase activity in vitro is O(2) sensitive, which indicates that respiration may play an important role in H(2) production. A sustainable production of H(2) was obtained for 200 h under illumination. Further, by using 3-(3,4-dichlorophenyl)-1,1-dimethylurea as a PSII inhibitor, at least 70% electrons for H(2) production were generated from PSII-catalyzed-H(2)O oxidation. The remaining electrons were probably from endogenous substrate degradation.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, China.
authors.forename: Huanyu
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Fan
authors.forename: Yanting
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.forename: Rongbo
authors.initials: R
authors.lastname: Guo
mesh_terms: D002708:Chlorella / Q000378:metabolism*; D003624:Darkness; D004237:Diuron / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D008027:Light; D045332:Photosystem II Protein Complex / Q000037:antagonists & inhibitors; D013455:Sulfur / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D045332:Photosystem II Protein Complex; D013455:Sulfur; D006859:Hydrogen; D004237:Diuron; D006864:Hydrogenase