title: Extracellular heat shock protein 90 plays a role in translocating chaperoned antigen from endosome to proteasome for generating antigenic peptide to be cross-presented by dendritic cells.
abstract: Extracellular heat shock protein can deliver associated antigens into the MHC class I presentation pathway of antigen-presenting cells, a process called cross-presentation, thus inducing antigen-specific CD8(+) T-cell responses; however, the precise mechanism for intracellular antigen translocation and the processing pathway has not been fully elucidated. Here we demonstrate that cross-presentation of extracellular Hsp90-ovalbumin (OVA) protein complexes to specific CD8(+) T cells involves both classical proteasome-transporter-associated antigen processing (TAP)-dependent and TAP-independent-endosomal pathways. Using confocal microscopy, we found that the internalized extracellular Hsp90 and OVA co-localized with cytosolic proteasomes. When anti-Hsp90 mAb was introduced to dendritic cells (DCs), we observed that the co-localization of internalized Hsp90-chaperoned OVA and proteasomes was abolished, resulting in the inhibition of TAP-dependent cross-presentation of OVA. Thus, extracellular Hsp90 may play a pivotal role for the translocation of chaperoned antigens for proteasomal degradation in the cytosol. In contrast, OVA chaperoned by Hsp90 was not presented by MHC class II molecules in vitro or in vivo, although the antigen was exogenously loaded onto DCs. Our data indicate that extracellular Hsp90 might be essential for the translocation of chaperoned antigens from the extracellular milieu into cytosol, resulting in proteasomal degradation for cross-presentation.
authors.affiliation: Department of Pathology, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo 060-8556, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Oura
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Tamura
authors.forename: Kenjirou
authors.initials: K
authors.lastname: Kamiguchi
authors.forename: Goro
authors.initials: G
authors.lastname: Kutomi
authors.forename: Hiroeki
authors.initials: H
authors.lastname: Sahara
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Torigoe
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Himi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D045142:Cross-Priming; D003600:Cytosol; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D005110:Extracellular Space; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism*; D006649:Histocompatibility Antigens / Q000378:metabolism; D006825:Hybridomas; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018832:Molecular Chaperones / Q000276:immunology / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport
chemical_list: D000941:Antigens; D018841:HSP90 Heat-Shock Proteins; D006649:Histocompatibility Antigens; D018832:Molecular Chaperones; D010446:Peptide Fragments; D010047:Ovalbumin; D046988:Proteasome Endopeptidase Complex