title: The diagnostic utility of p16 FISH and GLUT-1 immunohistochemical analysis in mesothelial proliferations.
abstract: Two promising ancillary tests used in the diagnosis of mesothelioma include GLUT-1 immunohistochemical analysis and fluorescence in situ hybridization (FISH) testing for the p16 deletion. This study compared the diagnostic usefulness of p16 FISH and GLUT-1 immunohistochemical analysis in the diagnosis of mesothelial proliferations in 158 cases with a diagnosis of benign (45.4%), atypical (10.4%), or malignant/mesothelioma (44.2%). Of the 70 benign cases, none had a deletion of p16 and 5 cases (7%) were positive for GLUT-1. Of the 68 mesotheliomas, 40 (59%) had a deletion of p16 (sensitivity, 59%; specificity, 100%) and 27 (40%) were positive for GLUT-1 (sensitivity, 40%; specificity, 93%). GLUT-1 showed lower sensitivity in pleural (56% vs 70%) and peritoneal (29% vs 51%) mesotheliomas (P = .004). Our results demonstrate that p16 FISH is a more sensitive and specific test than GLUT-1 immunohistochemical analysis and can be a more reliable ancillary tool to support the diagnosis of mesothelioma.
authors.affiliation: Dept of Pathology, University of Pittsburgh Medical Center, PA 15232, USA.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Monaco
authors.forename: Yongli
authors.initials: Y
authors.lastname: Shuai
authors.forename: Mona
authors.initials: M
authors.lastname: Bansal
authors.forename: Alyssa M
authors.initials: AM
authors.lastname: Krasinskas
authors.forename: Sanja
authors.initials: S
authors.lastname: Dacic
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D010534:Peritoneal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D010997:Pleural Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D011237:Predictive Value of Tests; D055815:Young Adult
chemical_list: D051272:Glucose Transporter Type 1