title: Crystal structures of the S. cerevisiae Spt6 core and C-terminal tandem SH2 domain.
abstract: The conserved and essential eukaryotic protein Spt6 functions in transcription elongation, chromatin maintenance, and RNA processing. Spt6 has three characterized functions. It is a histone chaperone capable of reassembling nucleosomes, a central component of transcription elongation complexes, and is required for recruitment of RNA processing factors to elongating RNA polymerase II (RNAPII). Here, we report multiple crystal structures of the 168-kDa Spt6 protein from Saccharomyces cerevisiae that together represent essentially all of the ordered sequence. Our two structures of the ∼900-residue core region reveal a series of putative nucleic acid and protein-protein interaction domains that fold into an elongated form that resembles the bacterial protein Tex. The similarity to a bacterial transcription factor suggests that the core domain performs nucleosome-independent activities, and as with Tex, we find that Spt6 binds DNA. Unlike Tex, however, the Spt6 S1 domain does not contribute to this activity. Crystal structures of the Spt6 C-terminal region reveal a tandem SH2 domain structure composed of two closely associated SH2 folds. One of these SH2 folds is cryptic, while the other shares striking structural similarity with metazoan SH2 domains and possesses structural features associated with the ability to bind phosphorylated substrates including phosphotyrosine. Binding studies with phosphopeptides that mimic the RNAPII C-terminal domain revealed affinities typical of other RNAPII C-terminal domain-binding proteins but did not indicate a specific interaction. Overall, these findings provide a structural foundation for understanding how Spt6 encodes several distinct functions within a single polypeptide chain.
authors.affiliation: Department of Biochemistry, University of Utah, Salt Lake City, UT 84112-5650, USA.
authors.forename: Devin
authors.initials: D
authors.lastname: Close
authors.forename: Sean J
authors.initials: SJ
authors.lastname: Johnson
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Sdano
authors.forename: Seth M
authors.initials: SM
authors.lastname: McDonald
authors.forename: Howard
authors.initials: H
authors.lastname: Robinson
authors.forename: Tim
authors.initials: T
authors.lastname: Formosa
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Hill
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D056488:Histone Chaperones; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry; D019000:Phosphotyrosine / Q000737:chemistry; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D035602:Transcriptional Elongation Factors / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D009687:Nuclear Proteins; D010750:Phosphoproteins; C046282:RNA polymerase II-binding proteins; C065456:SPT6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors; D019000:Phosphotyrosine