title: [Study on the effects of zoledronic-acid-loaded collagen membrane on bone metabolism cells].
abstract: OBJECTIVE
  To develop zoledronic acid (ZA)-loaded collagen membranes, and to study its effect on osteoclast and osteoblast so as to investigate whether ZA-loaded membranes can inhibit local bone resorption and promote bone formation.
  
  
  METHODS
  ZA-loaded double-layer (Bio-Gide(®)) and single-layer (BME-10X(®)) collagen membranes were prepared and divided into eight groups according to the concentrations of ZA in the membrane, namely Group BG0, BG1, BG2, BG3 and BM0, BM1, BM2, BM3 (BG refers to Bio-Gide(®), BM refers to BME-10X(®), 0, 1, 2, 3 refer to the concentrations of ZA, 0, 1 × 10(-4), 1 × 10(-3), 1 × 10(-2) mol/L respectively). Blank control group was set without using collagen membrane. The effects of ZA-loaded membranes on osteoclast and osteoblast were assessed using in vitro cell culture models.
  
  
  RESULTS
  In vitro coculture of ZA-loaded membrane with osteoclast for seven days showed that the percentage of bone resorption area in BG1, BG2, BG3, BM1, BM2, BM3 were 18.80%, 14.75%, 14.28%, 20.51%, 15.77%, 15.12% respectively, which were lower than that in BG0 (31.53%) and BM0 (32.22%, P < 0.05), and the higher ZA loading was, the stronger its inhibition to osteoclast was. In vitro coculture of ZA-loaded membrane with osteoblast for four days indicated that alkaline phosphatase (ALP) activities in BG2 (154.67 U/g), BM2 (154.33 U/g), BG3 (155.33 U/g), BM3 (152.00 U/g) were higher than that in BG0 (129.33 U/g) and BM0 (127.67 U/g, P < 0.05). What's more, results from seven-day coculture showed that proliferation index in BG2 (7.00) was higher than that in BG0 (6.90).
  
  
  CONCLUSIONS
  ZA-loaded collagen membrane can not only inhibit osteoclastic bone resorption but also improve proliferation of osteoblast.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China. Email: xingliangdent@vip.163.com.
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Meng
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Die
authors.initials: D
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000494:pharmacology; D001862:Bone Resorption / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004164:Diphosphonates / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology*; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis; D011817:Rabbits; D000077211:Zoledronic Acid
chemical_list: C106416:Bio-Gide; D001672:Biocompatible Materials; D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007093:Imidazoles; D008567:Membranes, Artificial; D000077211:Zoledronic Acid; D003094:Collagen; D000469:Alkaline Phosphatase