title: Mandibular alveolar bony defect repair using bone morphogenetic protein 2-expressing autologous mesenchymal stem cells.
abstract: BACKGROUND
  Mandibular bone regeneration is stepped up by human recombinant bone morphogenetic protein 2 (BMP-2) whose application is also related to limited cementum and periodontal ligament regeneration, local root resorption, and ankylosis. The alveolar bone grafting without traditional autologous bone grafts remains a challenge for plastic surgeons.
  
  
  METHODS
  Bilateral mandibular alveolar and periodontal defects were created over the premolar areas in 9 mature male beagles. The defects were randomly assigned for either the adenovirus BMP-2 (advBMP-2) group with BMP-2-expressing mesenchymal stem cells (MSCs) or the control with MSCs alone. The regenerated periodontal attachment apparatus was evaluated histologically, and the whole regenerated bone volume was scrutinized from three-dimensional computed tomography analysis.
  
  
  RESULTS
  Periodontal apparatus regeneration was significantly better in the advBMP-2 group. New cementum and Sharpey fibers were observed on the denuded root surfaces in the advBMP-2 group, whereas incomplete healing with localized root surface resorption was noted in the control group. Eight weeks after implantation, the advBMP-2 group showed significant increase in bone regeneration than the control one.
  
  
  CONCLUSIONS
  Thus, the use of ex vivo BMP-2-engineered autologous MSCs boosted bone and periodontal apparatus regeneration in mandibular periodontal defects. This de novo approach might be suitable for clinical mandibular bone repair and periodontal apparatus repair.
authors.affiliation: Department of Applied Chemistry, Providence University, and Department of Plastic Surgery, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Vanessa Hui-Ying
authors.initials: VH
authors.lastname: Chung
authors.forename: Amy Yi-Ling
authors.initials: AY
authors.lastname: Chen
authors.forename: Chang-Chin
authors.initials: CC
authors.lastname: Kwan
authors.forename: Philip Kao-Ting
authors.initials: PK
authors.lastname: Chen
authors.forename: Sophia Chia-Ning
authors.initials: SC
authors.lastname: Chang
mesh_terms: D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000601:surgery*; D000818:Animals; D015153:Blotting, Western; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D004195:Disease Models, Animal; D004285:Dogs; D021621:Imaging, Three-Dimensional; D008297:Male; D008334:Mandible / Q000601:surgery*; D059630:Mesenchymal Stem Cells; D017622:Periodontal Attachment Loss / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000601:surgery*; D011897:Random Allocation; D012038:Regeneration / Q000187:drug effects*; D014057:Tomography, X-Ray Computed
chemical_list: D055396:Bone Morphogenetic Protein 2