title: Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana.
abstract: The 5'-3' exoribonucleases (Xrns) play key roles in degradation and processing pathways of several classes of RNAs including mRNA, rRNA, miRNA and other small RNAs. Recent work revealed that the cytoplasmic Xrn (Xrn1p in yeast and Xrn4 in plants) affected the stability of the viral RNA of tombusviruses in yeast and plants, which indicates that the cytoplasmic Xrn might be involved in plant defense against virus by degrading viral RNA. Here, we demonstrated that silencing of Nicotiana benthamiana cytoplasmic Xrn4 facilitated both local and systemic infection of Tobacco mosaic virus (TMV) in N. benthamiana. The results support the suggestion that cytoplasmic Xrn4 participates in the viral defense system of plants.
authors.affiliation: Plant Protection College, Yunnan Agricultural University, Kunming 650205, China.
authors.forename: Jiejun
authors.initials: J
authors.lastname: Peng
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Lu
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jiang
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Hongying
authors.initials: H
authors.lastname: Zheng
authors.forename: Hairu
authors.initials: H
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000276:immunology* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*
chemical_list: D010940:Plant Proteins; D005095:Exoribonucleases