title: Preparation of quantum dots encoded microspheres by electrospray for the detection of biomolecules.
abstract: In this paper, a novel method based on the electrospray technique has been developed for preparation of quantum dot (QD)-encoded microspheres for the fist time. By electrospraying the mixture of polymer solution and quantum dots solution (single-color QDs or multi-color QDs), it is accessible to obtain a series of composite microspheres containing the functional nanoparticle. Poly(styrene-acrylate) was utilized as the electrospray polymer materials in order to obtain the microsphere modified with carboxyl group on the surface. Moreover, to test the performance of the QD-encoded microsphere in bioapplication, it is carried out that immunofluorescence analysis between antigens of mouse IgG immobilized on the functional microsphere and FITC labeled antibodies of goat-anti-mouse IgG in experiment. To the best of our knowledge, this is the first report of QD-encoded microspheres prepared by electrospray technology. This technology can carry out the one-pot preparation of different color QD-encoded microspheres with multiple intensities. This technology could be also suitable for encapsulating other optical nanocrystals and magnetic nanoparticles for obtaining multifunctional microspheres. All of the results in this paper show that the fluorescence beads made by electrospray technique can be well applied in multiplex analysis. These works provide a good foundation to accelerate application of preparing microspheres by electrospray technique in practice.
authors.affiliation: Department of Chemistry, Jilin University, Changchun, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Yu
authors.forename: Mingda
authors.initials: M
authors.lastname: Sun
authors.forename: Hengguo
authors.initials: H
authors.lastname: Wang
authors.forename: Shufei
authors.initials: S
authors.lastname: Xu
authors.forename: John D
authors.initials: JD
authors.lastname: Dixon
authors.forename: Y Andrew
authors.initials: YA
authors.lastname: Wang
authors.forename: Yaoxian
authors.initials: Y
authors.lastname: Li
authors.forename: Qingbiao
authors.initials: Q
authors.lastname: Yang
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D055664:Electrochemical Techniques / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D015200:Fluoroimmunoassay / Q000379:methods*; D006041:Goats; D007074:Immunoglobulin G / Q000032:analysis*; D051379:Mice; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D045663:Quantum Dots
chemical_list: D007074:Immunoglobulin G; D011137:Polystyrenes; D016650:Fluorescein-5-isothiocyanate