title: Protective effect of Justicia gendarussa Burm.f. on carrageenan-induced inflammation.
abstract: The present study focuses on the anti-inflammatory activity of Justicia gendarussa Burm.f. on an acute model of inflammation: the carrageenan-induced paw edema assay. Methanolic extract of J. gendarussa (JRM) roots at a dose of 100 mg/kg significantly inhibited edema formation 5 h after carrageenan induction. JRM inhibited carrageenan-elicited total cycloxygenase activity, 5-lipoxygenase and 15-lipoxygenase activities in blood mononuclear cells of rats, decreased neutrophil infiltration in paw tissue as shown by low myeloperoxidase activity, and also caused a 53% inhibition in cyclooxygenase-2 activity in paw tissue. Purification of JRM by liquid-liquid partitioning yielded an ethyl acetate fraction of JRM that showed interleukin-6 downregulation potential and the ability to inhibit prostaglandin E2 production in vivo. The present investigation provides pharmacological evidence to support the folklore claim that J. gendarussa is an anti-inflammatory agent.
authors.affiliation: Department of Biochemistry, University of Kerala, Thiruvananthapuram, Kerala, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Kavitha
authors.forename: V
authors.initials: V
authors.lastname: Viji
authors.forename: K
authors.initials: K
authors.lastname: Kripa
authors.forename: A
authors.initials: A
authors.lastname: Helen
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D002351:Carrageenan / Q000633:toxicity*; D015232:Dinoprostone / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D010936:Plant Extracts; D002351:Carrageenan; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone