title: Interplay of SLIM1 and miR395 in the regulation of sulfate assimilation in Arabidopsis.
abstract: MicroRNAs play a key role in the control of plant development and response to adverse environmental conditions. For example, microRNA395 (miR395), which targets three out of four isoforms of ATP sulfurylase, the first enzyme of sulfate assimilation, as well as a low-affinity sulfate transporter, SULTR2;1, is strongly induced by sulfate deficiency. However, other components of sulfate assimilation are induced by sulfate starvation, so that the role of miR395 is counterintuitive. Here, we describe the regulation of miR395 and its targets by sulfate starvation. We show that miR395 is important for the increased translocation of sulfate to the shoots during sulfate starvation. MiR395 together with the SULFUR LIMITATION 1 transcription factor maintain optimal levels of ATP sulfurylase transcripts to enable increased flux through the sulfate assimilation pathway in sulfate-deficient plants. Reduced expression of ATP sulfurylase (ATPS) alone affects both sulfate translocation and flux, but SULTR2;1 is important for the full rate of sulfate translocation to the shoots. Thus, miR395 is an integral part of the regulatory circuit controlling plant sulfate assimilation with a complex mechanism of action.
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich, UK.
authors.forename: Cintia G
authors.initials: CG
authors.lastname: Kawashima
authors.forename: Colette A
authors.initials: CA
authors.lastname: Matthewman
authors.forename: Siqi
authors.initials: S
authors.lastname: Huang
authors.forename: Bok-Rye
authors.initials: BR
authors.lastname: Lee
authors.forename: Naoko
authors.initials: N
authors.lastname: Yoshimoto
authors.forename: Anna
authors.initials: A
authors.lastname: Koprivova
authors.forename: Ignacio
authors.initials: I
authors.lastname: Rubio-Somoza
authors.forename: Marco
authors.initials: M
authors.lastname: Todesco
authors.forename: Tina
authors.initials: T
authors.lastname: Rathjen
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Tamas
authors.initials: T
authors.lastname: Dalmay
authors.forename: Stanislav
authors.initials: S
authors.lastname: Kopriva
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D056426:Genetic Loci; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D013430:Sulfate Adenylyltransferase / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C500268:EIL3 protein, Arabidopsis; D035683:MicroRNAs; D013431:Sulfates; D014157:Transcription Factors; D013430:Sulfate Adenylyltransferase