title: Induction of Per1 expression following an experimentally induced epilepsy in the mouse hippocampus.
abstract: The Period1 (Per1) is a clock-oscillating gene product that plays an essential role in the generation and modulation of circadian rhythm in the suprachiasmatic nucleus (SCN) of hypothalamus. However, Per1 is also expressed in many other brain regions including cerebral cortex, hippocampus, and amygdala, suggesting that Per1 may be involved in the broader cellular functions in addition to the rhythm regulation. In this study, we found that chemical or electrical seizure-inducing stimulations regulate Per1 expression. Treatments with electric convulsive shock (ECS) or kainic acid (KA) robustly up-regulated the expressions of per1 mRNA and protein in the hippocampal formation and cerebral cortex. In consistent, we found that neuronal depolarization or KA treatment increased per1 mRNA expression in cultured primary cortical neurons. Because it has been demonstrated that Per family molecules contribute to the regulation of stress-induced cell death, we also explored the effect of Per1 overexpression on the survival of cultured neurons. However, neither basal, staurosporine- nor KA-induced neuronal death was affected by forced expression of Per1. Collectively, these results suggest that the Per1 expression is neuronal activity- and epileptogen-dependent, although its functional significance is remained to be explored.
authors.affiliation: Department of Anatomy, Division of Brain Korea 21 Biomedical Science, Korea University College of Medicine, Anam-Dong, Sungbuk-Gu, Seoul, Republic of Korea.
authors.forename: Bokkee
authors.initials: B
authors.lastname: Eun
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.forename: Soon Taek
authors.initials: ST
authors.lastname: Hong
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Choi
authors.forename: Jae Kwang
authors.initials: JK
authors.lastname: Shim
authors.forename: Younghye
authors.initials: Y
authors.lastname: Moon
authors.forename: Gi Hoon
authors.initials: GH
authors.lastname: Son
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.forename: Woong
authors.initials: W
authors.lastname: Sun
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology; D004597:Electroshock; D004827:Epilepsy / Q000139:chemically induced / Q000209:etiology / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid / Q000633:toxicity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D056950:Period Circadian Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D019311:Staurosporine / Q000494:pharmacology
chemical_list: D018691:Excitatory Amino Acid Antagonists; D009419:Nerve Tissue Proteins; C539023:Per1 protein, mouse; C539024:Per1 protein, rat; D056950:Period Circadian Proteins; D011993:Recombinant Fusion Proteins; D011189:Potassium Chloride; D019311:Staurosporine; D007608:Kainic Acid