title: Compounds from Platycladus orientalis and their inhibitory effects on nitric oxide and TNF-α production.
abstract: Four new compounds: an abietane (1), two isopimarane (2 and 3) diterpenes, and a dihydrobenzofuran neolignan (5), together with a sesquiterpene glycoside (6) firstly isolated from a natural source, and a known phenol glycoside (4) were isolated from leaves of Platycladus orientalis. The structures and relative configurations of these compounds were assigned on the basis of MS, IR, 1D and 2D NMR data. The absolute configuration of compound 1 was determined via density functional theory calculations of its electronic circular dichroism. In addition, the results of bioassays indicated that compounds 4 and 6 showed a potent inhibitory effect on NO production with IC₅₀ values of 24.4 and 11.9 µM, respectively. Meanwhile, compounds 1, 2, and 3 moderately inhibited TNF- α release.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, P. R. China.
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Fan
authors.forename: Yue-Hu
authors.initials: YH
authors.lastname: Pei
authors.forename: Hua-Wu
authors.initials: HW
authors.lastname: Zeng
authors.forename: Shou-De
authors.initials: SD
authors.lastname: Zhang
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Ye
authors.forename: Yue-Xin
authors.initials: YX
authors.lastname: Pan
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Li
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D029779:Cupressaceae / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000009:adverse effects; D008516:Medicine, Chinese Traditional; D051379:Mice; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D017705:Lignans; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide