title: Leukemia inhibitory factor stimulates the transition of primordial to primary follicle and supports the goat primordial follicle viability in vitro.
abstract: The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) on the activation and survival of preantral follicles cultured in vitro enclosed in ovarian fragments (in situ). Goat ovarian cortex was divided into fragments to be used in this study. One fragment was immediately fixed (fresh control - FC) and the remaining fragments were cultured in supplemented minimum essential medium (MEM) without (cultured control - CC) or with different concentrations of LIF (1, 10, 50, 100 or 200 ng/ml) for 1 or 7 days, at 39°C in air with 5% CO2. Fresh control, CC and treated ovarian fragments were processed for histological and fluorescence analysis. The percentage of histological normal preantral follicles cultured for 7 days with 1 ng/ml (49.3%), 10 ng/ml (58.6%) and 50 ng/ml (58%) of LIF was higher than in the CC (32.6%; p < 0.05). After 7 days of culture, the percentage of primordial follicles in situ cultured with LIF decreased and primary follicles increased in all LIF concentrations compared with FC and CC (p < 0.05). In conclusion, LIF induced primordial follicle activation and supported preantral follicle viability of goat ovarian tissues cultured for 7 days.
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, CEP 97105-900, Santa Maria, RS, Brazil.
authors.forename: Janduí Escarião
authors.initials: JE
authors.lastname: da Nóbrega
authors.forename: Paulo Bayard Dias
authors.initials: PB
authors.lastname: Gonçalves
authors.forename: Roberta Nogueira
authors.initials: RN
authors.lastname: Chaves
authors.forename: Deborah de Melo
authors.initials: Dde M
authors.lastname: Magalhães
authors.forename: Rafael
authors.initials: R
authors.lastname: Rossetto
authors.forename: Isabel Bezerra
authors.initials: IB
authors.lastname: Lima-Verde
authors.forename: Gabriel Ribas
authors.initials: GR
authors.lastname: Pereira
authors.forename: Cláudio Cabral
authors.initials: CC
authors.lastname: Campello
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: João Francisco Coelho
authors.initials: JF
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D002470:Cell Survival; D003470:Culture Media / Q000378:metabolism; D005260:Female; D005453:Fluorescence; D006041:Goats / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D023421:Models, Animal; D009865:Oocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000662:veterinary*
chemical_list: D003470:Culture Media; D053605:Leukemia Inhibitory Factor