title: Phosphopeptides designed for 5-methylcytosine recognition.
abstract: An artificial phosphopeptide has been developed through rational design of the interaction with 5-methylcytosine in duplex DNA. The peptide consists of two tandem zinc finger motifs, in one of which the glutamate was replaced with a phosphotyrosine, the phosphotyrosine in the peptide being effective for methylcytosine selectivity of DNA binding. The flexible modulation of the target methylated sequence by rearrangement of zinc finger peptides is possible, and the phosphopeptide provided us an important hint for expansion of the codes for the interactions of zinc fingers with DNA to methylated DNA sequences. The fluorescence-labeled phosphopeptide provided information on the methylation status of genomic DNA through fluorescence anisotropy after a 10 min incubation.
authors.affiliation: Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Nomura
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry*; D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005454:Fluorescence Polarization; D019906:Nuclear Magnetic Resonance, Biomolecular; D010748:Phosphopeptides / Q000138:chemical synthesis / Q000378:metabolism*; D016329:Sp1 Transcription Factor / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D010748:Phosphopeptides; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C492254:ZBTB33 protein, human; D044503:5-Methylcytosine