title: Mycobacterium bovis antigens for the differential diagnosis of vaccinated and infected cattle.
abstract: The urgency for new and improved cattle vaccines and diagnostic reagents for Bovine tuberculosis (TB) has made their development a research priority in Great Britain (GB). Significant progress has been made to develop specific antigens that allow the differentiation of BCG vaccinated and Mycobacterium bovis infected cattle (DIVA test). This has been greatly facilitated by the completion of the genome sequences of M. tuberculosis, M. bovis and BCG Pasteur and the subsequent application of comparative genome and transcriptome analysis to define DIVA antigens that complemented the prototype DIVA antigens ESAT-6 and CFP-10 by increasing their test sensitivity. Finally, we present an up-date of our current approaches in this area.
authors.affiliation: Veterinary Laboratories Agency - Weybridge, TB Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK. m.vordermeier@vla.defra.gsi.gov.uk
authors.forename: Martin
authors.initials: M
authors.lastname: Vordermeier
authors.forename: Stephen V
authors.initials: SV
authors.lastname: Gordon
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D002417:Cattle / Q000382:microbiology*; D055028:Comparative Genomic Hybridization; D003937:Diagnosis, Differential; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control; D006113:United Kingdom
chemical_list: D000942:Antigens, Bacterial; D032581:Tuberculosis Vaccines