title: A novel real-time polymerase chain reaction for identification of Salmonella enterica subspecies enterica.
abstract: Salmonella enterica subspecies enterica (subspecies I) causes the majority of infections in humans and homeothermic animals. We present a real-time polymerase chain reaction assay targeting the hilA gene that demonstrates 97.9% specificity and 99.9% sensitivity for rapid and reliable identification of subspecies I, offering savings in time and labor over traditional methods.
authors.affiliation: Laboratory of Gastrointestinal Pathogens, Health Protection Agency Centre for Infections, 61 Colindale Avenue, NW9 5EQ, United Kingdom. katie.hopkins@hpa.org.uk
authors.forename: Katie L
authors.initials: KL
authors.lastname: Hopkins
authors.forename: Andy J
authors.initials: AJ
authors.lastname: Lawson
authors.forename: Steve
authors.initials: S
authors.lastname: Connell
authors.forename: Tansy M
authors.initials: TM
authors.lastname: Peters
authors.forename: Elizabeth
authors.initials: E
authors.lastname: de Pinna
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012480:Salmonella Infections / Q000175:diagnosis* / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C102236:HilA protein, Salmonella; D015534:Trans-Activators