title: Separation, identification and quantification of tetrodotoxin and its analogs by LC-MS without calibration of individual analogs.
abstract: We report here a simple and rapid method of separation, identification and quantification of tetrodotoxin (TTX) and its analogs in partially purified extract by LC-MS. Except for the main component calibration of individual components was not necessary. TTX and four of its analogs in the puffer fish extract were identified and quantified. The limits of detection and quantification, the linear range and accuracy of the protocol were validated.
authors.affiliation: Key Laboratory of Chemical Biology of Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518057, PR China.
authors.forename: Xiao-Wu
authors.initials: XW
authors.lastname: Chen
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Liu
authors.forename: Yi-Bao
authors.initials: YB
authors.lastname: Jin
authors.forename: Shang-Fu
authors.initials: SF
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Bi
authors.forename: Stephen
authors.initials: S
authors.lastname: Chung
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Zhang
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Jiang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013779:Tetrodotoxin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D013779:Tetrodotoxin