title: Antibacterial activity of the enniatin B, produced by Fusarium tricinctum in liquid culture, and cytotoxic effects on Caco-2 cells.
abstract: The enniatins (ENs) are bioactive compounds of hexadepsipeptidic structure produced by several strains of Fusarium sp. The EN B was purified from extracts of Fusarium tricinctum growth on liquid culture of potato dextrose broth (PDB), using a semipreparative liquid chromatography (LC) followed by an analytical LC. The purity and the structure of the isolated compound were confirmed by the determination of the extinction coefficient and with electrospray ionization-mass spectrometry (ESI-MS) study. The pure fraction of EN B was utilized to determine the antibiotic effects on several bacterial strains that are considered normally pathogens of the intestinal tract: Escherichia coli, Enterococcus faecium, Salmonella enterica, Shigella dysenteriae, Listeria monocytogenes, Yersinia enterocolitica, Clostridium perfringens, Pseudomonas aeruginosa, and Staphylococcus aureus, and to study the cytotoxic effects on Caco-2 differentiated and undifferentiated cells. The results obtained demonstrated that in several antibiograms, EN B induced the inhibition of the grown microorganisms tested and no significant differences over control were detected when Caco-2 cells were exposed to EN B, at any of the concentrations used.
authors.affiliation: Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Avenue Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain. giuseppe.meca@uv.es
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Meca
authors.forename: Isabel
authors.initials: I
authors.lastname: Sospedra
authors.forename: María Adela
authors.initials: MA
authors.lastname: Valero
authors.forename: Jordi
authors.initials: J
authors.lastname: Mañes
authors.forename: Guillermina
authors.initials: G
authors.lastname: Font
authors.forename: María José
authors.initials: MJ
authors.lastname: Ruiz
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D047630:Depsipeptides / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D047630:Depsipeptides; D008055:Lipids; C100264:enniatins