title: Comparison between immunohistochemistry and genetic clonality analysis for cellular lineage determination in feline lymphomas.
abstract: Clinical application of genetic clonality analysis by PCR has been available in feline lymphoma. However, it is in under dispute whether the analysis can be applied for cellular lineage determination. To evaluate utility of the analysis for lineage determination, the results between immunohistochemistry and the genetic analysis were compared. Based on the result with immunohistochemistry, sensitivity of the primer for IgH was 89% and specificity was 75%. Sensitivity of the primer for TCRγ was 25% and specificity was 100%. Cross-lineage IgH rearrangement was found in 25% of T-cell lymphomas. The present study suggests that the genetic analysis had better be applied as not a definite but an adjunctive tool for lineage determination in feline lymphomas.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sato
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000235:genetics* / Q000473:pathology*; D002415:Cats; D019070:Cell Lineage / Q000235:genetics*; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005803:Genes, Immunoglobulin / Q000235:genetics; D019675:Genes, T-Cell Receptor gamma; D007150:Immunohistochemistry / Q000662:veterinary; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm