title: Intragastric administration of allyl isothiocyanate increases carbohydrate oxidation via TRPV1 but not TRPA1 in mice.
abstract: The transient receptor potential (TRP) channel family is composed of a wide variety of cation-permeable channels activated polymodally by various stimuli and is implicated in a variety of cellular functions. Recent investigations have revealed that activation of TRP channels is involved not only in nociception and thermosensation but also in thermoregulation and energy metabolism. We investigated the effect of intragastric administration of TRP channel agonists on changes in energy substrate utilization of mice. Intragastric administration of allyl isothiocyanate (AITC; a typical TRPA1 agonist) markedly increased carbohydrate oxidation but did not affect oxygen consumption. To examine whether TRP channels mediate this increase in carbohydrate oxidation, we used TRPA1 and TRPV1 knockout (KO) mice. Intragastric administration of AITC increased carbohydrate oxidation in TRPA1 KO mice but not in TRPV1 KO mice. Furthermore, AITC dose-dependently increased intracellular calcium ion concentration in cells expressing TRPV1. These findings suggest that AITC might activate TRPV1 and that AITC increased carbohydrate oxidation via TRPV1.
authors.affiliation: Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Yoshidahonmachi, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Mori
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kawabata
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Matsumura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosokawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Inoue
authors.forename: Tohru
authors.initials: T
authors.lastname: Fushiki
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects* / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology; D017879:Isothiocyanates / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D010084:Oxidation-Reduction; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels / Q000172:deficiency / Q000187:drug effects / Q000502:physiology*; D050051:Transient Receptor Potential Channels / Q000819:agonists / Q000172:deficiency / Q000502:physiology*
chemical_list: D017879:Isothiocyanates; D000074025:TRPA1 Cation Channel; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D050051:Transient Receptor Potential Channels; C475685:Trpa1 protein, mouse; D000171:Acrolein; C004471:allyl isothiocyanate; C012843:cinnamaldehyde