title: Antibody-enzyme conjugate formation.
abstract: The ideal antibody-enzyme conjugate retains all the immunoreactivity and enzyme activity of its components, has defined and appropriate proportions, and is stable. There are many methods of labeling immunoglobulins with enzymes, but none meet all these criteria (1). Functional groups in the protein, such as amino, thiol, or saccharide groups, are exploited, and in nearly all cases bifunctional reagents are used to link the enzyme to the immunoglobulin G (IgG). The three most commonly used methods are presented here with minor modifications to the originally described procedures.
authors.affiliation: Department of Biochemistry, The Queen's University of Belfast, Medical Biology Centre, Belfast, Ireland, UK.
authors.forename: G B
authors.initials: GB
authors.lastname: Wisdom