title: A biocompatible model for evaluation of the responses of rat periapical tissue to a new zinc oxide-eugenol sealer.
abstract: We aimed to establish an experimental animal model to evaluate materials for endodontic therapy. We focused on the biocompatibility of new paste-type zinc oxide-eugenol (ZOE) sealer. The results of this sealer were compared with those of conventional powder/liquid ZOE and eugenol-free sealers. The molars of Wistar rats were extracted and repositioned in the original socket after application of the sealers on the root apices. Mild inflammation occurred in the periapical tissue of the replanted teeth with both ZOE sealers on day 7, whereas the eugenol-free sealer induced severe inflammation. On day 14, the lesions induced by all types of sealers were healed and replaced predominantly by fibrous connective tissue. Thus, all endodontic materials showed high biocompatibility, although the extent of inflammatory reactions during the early stages varied depending on the types of materials. We demonstrated that our animal model was useful for the assessment of the biocompatibility of endodontic materials.
authors.affiliation: Department of Oral Medicine, Division of Endodontics, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Mutoh
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tani-Ishii
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D003738:Dental Cements / Q000494:pharmacology; D003739:Dental Cementum / Q000187:drug effects / Q000473:pathology; D003804:Dentin / Q000187:drug effects / Q000473:pathology; D005260:Female; D023421:Models, Animal; D008963:Molar / Q000473:pathology; D010485:Periapical Periodontitis / Q000139:chemically induced; D010486:Periapical Tissue / Q000187:drug effects* / Q000473:pathology; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012391:Root Resorption / Q000139:chemically induced; D013997:Time Factors; D019227:Tooth Apex / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects; D015034:Zinc Oxide / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D003738:Dental Cements; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C112894:canals sealer; C449162:zinc oxide-non-eugenol cement; D015034:Zinc Oxide