title: [The effect of CTLA4-Ig gene modified dendritic cell on Th1/Th2 balance in vitro].
abstract: OBJECTIVE
  To investigate the impact on Th1/Th2 subset balance from cytotoxic T lymphocyte-associated antigen immunoglobulin (CTLA4 Ig) gene-modified dendritic cells (CTLA4 Ig-DCs) in vitro.
  
  
  METHODS
  The modified DCs (CTIA4 Ig-DCs) were prepared by transferring the mouse bone marrow-derived DCs with the constructed adenovirus CTIA4 Ig vectorî The CTLA4 Ig expression and certain cell surface molecules on the CTIA4Ig-DCs were detected by FCM, the potential to stimulate allogeneic T cell proliferation of the modified DCs by mixed lymphocyte reaction (MLR) and the secretion of IFN-γ and IL-4 in antigen presentation by ELISA.
  
  
  RESULTS
  CTLA4 Ig gene was successfully transfected into DCs with stable expression of CTLA4 Ig, and its transfection efficiency was about 80%. Compared with the controls, CTLA4 Ig-DCs showed lower surface molecule CD86, inhibited allogeneic lymphocyte proliferation in MLR, decreased secretion of IFN-γ and IL-4 and increased ratio of IFN-γ/IL-4 in antigen presentation.
  
  
  CONCLUSIONS
  CTLA4 Ig-DCs were successfully obtained and effectively expressed CTLA4 Ig, which could reduce the expression of CD86 on DCs surface. CTLA4 Ig-DCs inhibited allogeneic T cell proliferation and affected the ratio of Th1/Th2 in vitro.
authors.affiliation: Department of Respiratory Center, Children's Hospital, Chongqing Medical University, Chongqing 400014, China. yinglinyan2006@yahoo.com.cn
authors.forename: Lin-yan
authors.initials: LY
authors.lastname: Ying
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fu
authors.forename: Jian
authors.initials: J
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: En-mei
authors.initials: EM
authors.lastname: Liu
authors.forename: Li-jia
authors.initials: LJ
authors.lastname: Wang
mesh_terms: D000069594:Abatacept; D000818:Animals; D051940:B7-2 Antigen / Q000378:metabolism*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D003713:Dendritic Cells / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D018796:Immunoconjugates / Q000235:genetics*; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016176:T-Lymphocyte Subsets / Q000378:metabolism / Q000473:pathology*; D058408:Th1-Th2 Balance
chemical_list: D051940:B7-2 Antigen; D018796:Immunoconjugates; D015847:Interleukin-4; D000069594:Abatacept; D007371:Interferon-gamma