title: Antihyperalgesic activity of verbascoside in two models of neuropathic pain.
abstract: OBJECTIVE
  This study reports on the rapid isolation of verbascoside from Lippia citriodora H.B.K. (Verbenaceae), an inexpensive and widespread source, and the evaluation of its antihyperalgesic activity.
  
  
  METHODS
  Isolation of verbascoside was achieved by size exclusion chromatography with Sephadex LH-20 eluting with 50% EtOH, which is proposed as a fast and efficient method of separation.
  
  
  RESULTS
  The antihyperalgesic activity of verbascoside was tested by in-vivo assay using the paw-pressure test in two animal models of neuropathic pain: a peripheral mononeuropathy produced either by a chronic constriction injury of the sciatic nerve (CCI) or by an intra-articular injection of sodium monoiodoacetate (MIA).
  
  
  CONCLUSIONS
  Verbascoside administered intraperitoneally at a dose of 100 mg/kg reverted the mechanical hyperalgesia in both CCI and MIA treated rats, as evaluated in the paw-pressure test. Verbascoside was also effective against mechanical hyperalgesia after oral administration at doses of 300 and 600 mg/kg.
authors.affiliation: Department of Pharmaceutical Sciences, University of Florence, Sesto Fiorentino Department of Preclinical and Clinical Pharmacology, University of Florence, Florence, Italy. benedetta.isacchi@unifi.it
authors.forename: Benedetta
authors.initials: B
authors.lastname: Isacchi
authors.forename: Romina
authors.initials: R
authors.lastname: Iacopi
authors.forename: Maria Camilla
authors.initials: MC
authors.lastname: Bergonzi
authors.forename: Carla
authors.initials: C
authors.lastname: Ghelardini
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Galeotti
authors.forename: Monica
authors.initials: M
authors.lastname: Norcini
authors.forename: Elisa
authors.initials: E
authors.lastname: Vivoli
authors.forename: Franco Francesco
authors.initials: FF
authors.lastname: Vincieri
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Bilia
mesh_terms: D000284:Administration, Oral; D000700:Analgesics / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D005960:Glucosides / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D006930:Hyperalgesia / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy*; D007274:Injections, Intraperitoneal; D007461:Iodoacetates; D032411:Lippia / Q000737:chemistry; D008297:Male; D009437:Neuralgia / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy*; D010636:Phenols / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D045442:Rotarod Performance Test / Q000379:methods; D012584:Sciatic Nerve / Q000293:injuries; D059808:Polyphenols
chemical_list: D000700:Analgesics; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007461:Iodoacetates; D010636:Phenols; C058956:acteoside; D059808:Polyphenols