title: Lipid peroxidation in all-in-one admixtures for preterm neonates: impact of amount of lipid, type of lipid emulsion and delivery condition.
abstract: OBJECTIVE
  To evaluate the effect of lipid emulsion composition and delivery condition on lipid peroxidation in typical all-in-one parenteral admixtures for preterm neonates.
  
  
  METHODS
  Malonedialdehyde (MDA) concentrations were assessed in different all-in-one admixtures. We evaluated the effects of fat blend (three lipid emulsions) and the amount of lipids, as well as the effects of protecting bags and/or tubing from ambient light and storage for 72 h. MDA was measured by liquid chromatography/mass spectrometry.
  
  
  RESULTS
  Three hundred and sixty samples were collected from 114 admixtures. Neither the type of lipid (p = 0.43) nor the interaction between light and type of lipid (p = 0.49) had any influence on final MDA concentrations, but the increase in MDA concentration at 24 h (T(24)) was related to light exposure (p < 0.001). The increase in MDA concentration was related to the increase in lipid amount in the admixture at T(0) (r = 0.77) and T(24) (r = 0.86). MDA concentrations in solutions stored for 72 h showed no significant increase, with no difference between the three lipid emulsions (p = 0.69).
  
  
  CONCLUSIONS
  All-in-one admixtures may be interesting for the parenteral nutrition of preterm neonates. Protection from light and restricting the amount of lipid to what is required for appropriate energy provision are essential to limit lipid peroxidation.
authors.affiliation: Department of Pharmacy, CHU de Montpellier, Hôpital Lapeyronie, Montpellier, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Jalabert
authors.forename: Anaïs
authors.initials: A
authors.lastname: Grand
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Steghens
authors.forename: Eric
authors.initials: E
authors.lastname: Barbotte
authors.forename: Christelle
authors.initials: C
authors.lastname: Pigue
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Picaud
mesh_terms: D000704:Analysis of Variance; D004355:Drug Stability; D004655:Emulsions; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007229:Infant Welfare; D007231:Infant, Newborn; D007234:Infant, Premature; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D009752:Nutritional Status; D010288:Parenteral Nutrition; D013223:Statistics as Topic; D018709:Statistics, Nonparametric
chemical_list: D004655:Emulsions; D005217:Fat Emulsions, Intravenous; D008315:Malondialdehyde