title: The dengue virus nonstructural protein 1 (NS1) increases NF-κB transcriptional activity in HepG2 cells.
abstract: Dengue virus nonstructural protein 1 (NS1) is a glycoprotein involved in viral RNA replication. NS1 associates with host cell proteins and can be found in lipid raft domains on the host cell surface, suggesting an involvement in signal transduction events. In this work, we observed that NS1 expression in HepG2 cells increases nuclear translocation of NF-κB p65 protein, which was paralleled by DNA-protein complex formation. Luciferase assays showed an increase in NF-κB transcriptional activities in NS1-expressing cells when compared to parental cells. NS1 may enhance NF-κB function in host cells and contribute to the pathogenesis of dengue.
authors.affiliation: Grupo de Transdução de Sinal, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. brenomello@pq.cnpq.br
authors.forename: Breno M
authors.initials: BM
authors.lastname: Silva
authors.forename: Lirlândia P
authors.initials: LP
authors.lastname: Sousa
authors.forename: Alessandra C
authors.initials: AC
authors.lastname: Gomes-Ruiz
authors.forename: Flávia G G
authors.initials: FG
authors.lastname: Leite
authors.forename: Mauro M
authors.initials: MM
authors.lastname: Teixeira
authors.forename: Flávio G
authors.initials: FG
authors.lastname: da Fonseca
authors.forename: Paulo F P
authors.initials: PF
authors.lastname: Pimenta
authors.forename: Paulo C P
authors.initials: PC
authors.lastname: Ferreira
authors.forename: Erna G
authors.initials: EG
authors.lastname: Kroon
authors.forename: Cláudio A
authors.initials: CA
authors.lastname: Bonjardim
mesh_terms: D003715:Dengue / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D006801:Humans; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; C082515:NS1 protein, dengue-1 virus; D017361:Viral Nonstructural Proteins