title: Evaluation of two molecular methods for the detection of Yellow fever virus genome.
abstract: Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(®) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
authors.affiliation: Department of Arbovirology and Hemorrhagic Fevers, Instituto Evandro Chagas, Ministry of Health, Ananindeua, Brazil. marcionunes@iec.pa.gov.br
authors.forename: Marcio R T
authors.initials: MR
authors.lastname: Nunes
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: Keley N B
authors.initials: KN
authors.lastname: Nunes
authors.forename: Samir M M
authors.initials: SM
authors.lastname: Casseb
authors.forename: Lívia C
authors.initials: LC
authors.lastname: Martins
authors.forename: Juarez A S
authors.initials: JA
authors.lastname: Quaresma
authors.forename: Nazir
authors.initials: N
authors.lastname: Savji
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
authors.forename: Pedro F C
authors.initials: PF
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D006224:Cricetinae; D006801:Humans; D008647:Mesocricetus; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D044967:Serum / Q000821:virology; D013020:South America; D014773:Virology / Q000379:methods*; D015004:Yellow Fever / Q000175:diagnosis* / Q000821:virology; D015005:Yellow fever virus / Q000235:genetics / Q000302:isolation & purification*