title: Ovine serum immunoglobulin has immunomodulatory effects in growing rats gavaged with Salmonella enteritidis.
abstract: In this study, we aimed to determine whether orally administered ovine serum Ig modulate aspects of immunity and associated gut microflora in growing rats challenged with Salmonella enteritidis. The 4 groups consisted of rats fed a casein-based control diet (BD; ungavaged) and 3 groups of rats gavaged with 1 × 10(7) viable Salmonella enteritidis and fed a BD diet, a BD diet containing freeze-dried ovine Ig (FDOI), or a BD diet containing inactivated ovine Ig (IOI). The rats were randomly allocated to 1 of the 4 diets (n = 15) and consumed it for 18 d. They were orally gavaged on d 15. Phagocytic activity of peripheral blood leukocyte and lymphocyte proliferation in the presence of the concanavalin A (ConA) were greater (P < 0.05) in the ungavaged BD- and gavaged FDOI-fed rats than in the gavaged rats fed either the BD or IOI diet. ConA-stimulated Peyer's patch cells and splenocytes from the gavaged rats fed the FDOI diet produced more IFNγ, IgA, and IgG than the gavaged rats fed either the BD or IOI diet (P < 0.05). The gavaged FDOI-fed rats had higher ileal and colonic digesta and plasma concentrations of anti-Salmonella secretory sIgA and secretory sIgG (P < 0.05). DNA analysis of a denatured gradient gel electrophoresis profile revealed that 6 of 10 bands had sequence similarity to probiotic strains of bacteria in the ileum and colon of the gavaged FDOI-fed rats. In conclusion, an ovine Ig fraction modulated various indices of immune function and associated gut microflora in growing rats inoculated with Salmonella.
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
authors.forename: Prabhu
authors.initials: P
authors.lastname: Balan
authors.forename: Kyoung-Sik
authors.initials: KS
authors.lastname: Han
authors.forename: Kay
authors.initials: K
authors.lastname: Rutherfurd-Markwick
authors.forename: Harjinder
authors.initials: H
authors.lastname: Singh
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Moughan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000378:metabolism; D002478:Cells, Cultured; D057899:Cytophagocytosis / Q000276:immunology; D005759:Gastroenteritis / Q000276:immunology / Q000517:prevention & control*; D005766:Gastrointestinal Contents / Q000737:chemistry / Q000382:microbiology; D054884:Host-Pathogen Interactions; D007136:Immunoglobulins / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D056747:Immunomodulation; D007371:Interferon-gamma / Q000378:metabolism; D007422:Intestines / Q000254:growth & development / Q000276:immunology / Q000382:microbiology; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012480:Salmonella Infections / Q000276:immunology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000276:immunology*; D034561:Sheep, Domestic; D013154:Spleen / Q000166:cytology / Q000254:growth & development / Q000276:immunology / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D007136:Immunoglobulins; D007371:Interferon-gamma