title: Development of inactivated trivalent vaccine for the teratogenic Aino, Akabane and Chuzan viruses.
abstract: Aino, Akabane and Chuzan viruses are arthropod-borne (arbo) viruses transmitted by blood-sucking insects like mosquitoes and Culicoides biting midges. These arbovirus infections are mainly associated with abortion, stillbirth and congenital defects in pregnant cattle, sheep and goats, which induces a considerable economic loss in livestock industry. The viruses seem to be widely distributed in Southeast Asia and Australia. As a control strategy, an inactivated trivalent vaccine against Aino, Akabane and Chuzan virus was developed by using binary ethylenimine or formalin as an inactivating agent. The newly developed trivalent vaccine is evaluated for its safety and immunogenicity in animals such as mice, guinea pigs and cattle. The immune responses were significantly detected within 2-weeks after second vaccination without any side effects. Since the field application of experimental vaccine also revealed increased antibodies in inoculated cattle, we demonstrated that these trivalent vaccines could be used as a vaccine to control the arboviral infections in ruminants.
authors.affiliation: National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Kweon
authors.forename: Dong-Seob
authors.initials: DS
authors.lastname: Tark
authors.forename: Seong In
authors.initials: SI
authors.lastname: Lim
authors.forename: Dong-Kun
authors.initials: DK
authors.lastname: Yang
authors.forename: Bang-Hun
authors.initials: BH
authors.lastname: Hyun
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Won
authors.initials: W
authors.lastname: Hur
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002044:Bunyaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D005260:Female; D005557:Formaldehyde; D015511:Goat Diseases / Q000276:immunology / Q000517:prevention & control; D006041:Goats; D006168:Guinea Pigs; D051379:Mice; D008813:Mice, Inbred ICR; D016851:Orthobunyavirus / Q000276:immunology* / Q000472:pathogenicity; D030081:Palyam Virus / Q000276:immunology* / Q000472:pathogenicity; D011247:Pregnancy; D012088:Reoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000302:isolation & purification; D014765:Viral Vaccines / Q000008:administration & dosage / Q000302:isolation & purification*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D005557:Formaldehyde