title: CD40L is not involved in acute experimental pancreatitis.
abstract: Recent data suggest that platelets not only control thrombosis and hemostasis but may also regulate inflammatory processes such as acute pancreatitis. However, the specific role of platelet-derived mediators in the pathophysiology of acute pancreatitis is not known. Herein, we examined the role of CD40 ligand (CD40L, CD154) in different models of acute pancreatitis. Acute pancreatitis was induced by repetitive caerulein administration (50μg/kg, i.p.) or infusion of sodium taurocholate (5%-10μl) into the pancreatic duct in wild-type C57BL/6 and CD40L-deficient mice. Neutrophil infiltration, myeloperoxidase (MPO), macrophage inflammatory protein-2 (MIP-2) levels, acinar cell necrosis, edema and hemorrhage in the pancreas as well as serum amylase activity and lung levels of MPO were quantified 24h after induction of acute pancreatitis. Caerulein and taurocholate challenge caused a clear-cut pancreatic damage characterized by increased acinar cell necrosis, neutrophil infiltration, focal hemorrhage, edema formation as well as increased levels of serum amylase and MIP-2 in the pancreas and lung MPO and histological damage. Notably, CD40L gene-deficient animals exhibited a similar phenotype as wild-type mice after challenge with caerulein and taurocholate. Similarly, administration of an antibody directed against CD40L had no effect against acute pancreatitis. Our data suggest that CD40L does not play a functional role in experimental acute pancreatitis. Thus, other candidates than CD40L needs to be explored in order to identify platelet-derived mediators in the pathophysiology of acute pancreatitis.
authors.forename: Aree
authors.initials: A
authors.lastname: Abdulla
authors.forename: Darbaz
authors.initials: D
authors.lastname: Awla
authors.forename: Bengt
authors.initials: B
authors.lastname: Jeppsson
authors.forename: Sara
authors.initials: S
authors.lastname: Regnér
authors.forename: Henrik
authors.initials: H
authors.lastname: Thorlacius
mesh_terms: D000208:Acute Disease; D000818:Animals; D023201:CD40 Ligand / Q000378:metabolism*; D002108:Ceruletide / Q000009:adverse effects; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism*; D013656:Taurocholic Acid / Q000009:adverse effects
chemical_list: D023201:CD40 Ligand; D013656:Taurocholic Acid; D002108:Ceruletide