title: [Identification and analysis of vaginal lactobacilli in patients with bacterial vaginosis patients and healthy women in nationality of Pastoral area].
abstract: OBJECTIVE
  To identify and analyze the species of vaginal lactobacilli between patients with bacterial vaginosis (BV) and healthy women at childbearing age in Inner Mongolia.
  
  
  METHODS
  From Jun. 2008 to Dec. 2008, 203 Mongolian healthy women, 74 Han healthy women and 102 Mongolian patients with BV from 3 pastoral areas were enrolled in this study. Isolation and culture of lactobacilli from vaginal wall were performed by modified culture medium. DNA of lactobacilli were extracted and sequenced. H₂O₂ were detected by TMB-HRP-MRS.
  
  
  RESULTS
  (1) The rate of lactobacilli identification were 76.8% (156/203) in Mongolian healthy women and 21.6% (22/102) in Mongolian patients with BV, which reached statistical difference (P < 0.01). Lactobacilli identification in Han healthy women [82.4% (61/74)] did not show significant difference with that of Mongolian healthy women (P > 0.05). (2) The total of 193 strains and 11 species of Lactobacillus were detected in 203 Mongolian healthy women. Meanwhile, 22 strains and 4 species of Lactobacillus were found in 102 Mongolian BV cases. (3) The rate of H₂O₂ generating Lactobacilli was 27.3% (6/22) in Mongolian BV patients and 75.7% (56/74) in Mongolian healthy women, which showed statistical difference (P < 0.05).
  
  
  CONCLUSIONS
  The rate of Lactobacillus was not related with the race of women in pastoral area in Inner Mongolian. The amount of lactobacilli and H₂O₂ generating Lactobacilli in the vagina of BV patients was remarkably lower than those of healthy women at childbearing age.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Inner Mongolia Medical College, Huhhot 010059, China. songjinghui2002@yahoo.com.cn
authors.forename: Jing-hui
authors.initials: JH
authors.lastname: Song
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Zheng
authors.forename: Hai-ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Ya
authors.initials: Y
authors.lastname: Tuo
authors.forename: Shu-fang
authors.initials: SF
authors.lastname: Song
mesh_terms: D000328:Adult; D002681:China; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000302:isolation & purification / Q000378:metabolism; D048668:Lactobacillus delbrueckii / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D014621:Vagina / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000382:microbiology*; D016387:Women's Health
chemical_list: D012336:RNA, Ribosomal, 16S; D006861:Hydrogen Peroxide