title: Arrhythmogenic substrate in hearts of rats with monocrotaline-induced pulmonary hypertension and right ventricular hypertrophy.
abstract: Mechanisms associated with right ventricular (RV) hypertension and arrhythmias are less understood than those in the left ventricle (LV). The aim of our study was to investigate whether and by what mechanisms a proarrhythmic substrate exists in a rat model of RV hypertension and hypertrophy. Rats were injected with monocrotaline (MCT; 60 mg/kg) to induce pulmonary artery hypertension or with saline (CON). Myocardial levels of mRNA for genes expressing ion channels were measured by real-time RT-PCR. Monophasic action potential duration (MAPD) was recorded in isolated Langendorff-perfused hearts. MAPD restitution was measured, and arrhythmias were induced by burst stimulation. Twenty-two to twenty-six days after treatment, MCT animals had RV hypertension, hypertrophy, and decreased ejection fractions compared with CON. A greater proportion of MCT hearts developed sustained ventricular tachycardias/fibrillation (0.83 MCT vs. 0.14 CON). MAPD was prolonged in RV and less so in the LV of MCT hearts. There were decreased levels of mRNA for K(+) channels. Restitution curves of MCT RV were steeper than CON RV or either LV. Dispersion of MAPD was greater in MCT hearts and was dependent on stimulation frequency. Computer simulations based on ion channel gene expression closely predicted experimental changes in MAPD and restitution. We have identified a proarrhythmic substrate in the hearts of MCT-treated rats. We conclude that steeper RV electrical restitution and rate-dependant RV-LV action potential duration dispersion may be contributing mechanisms and be implicated in the generation of arrhythmias associated with in RV hypertension and hypertrophy.
authors.affiliation: Institute of Membrane and Systems Biology, Garstang Bldg., Univ. of Leeds, Leeds LS29JT, UK.
authors.forename: David
authors.initials: D
authors.lastname: Benoist
authors.forename: Rachel
authors.initials: R
authors.lastname: Stones
authors.forename: Mark
authors.initials: M
authors.lastname: Drinkhill
authors.forename: Olivier
authors.initials: O
authors.lastname: Bernus
authors.forename: Ed
authors.initials: E
authors.lastname: White
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D015220:Calcium Channels / Q000502:physiology; D003198:Computer Simulation; D004195:Disease Models, Animal; D004558:Electric Stimulation; D006976:Hypertension, Pulmonary / Q000139:chemically induced* / Q000503:physiopathology*; D017380:Hypertrophy, Right Ventricular / Q000139:chemically induced* / Q000503:physiopathology*; D008297:Male; D016686:Monocrotaline / Q000009:adverse effects*; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013318:Stroke Volume / Q000502:physiology; D017180:Tachycardia, Ventricular / Q000503:physiopathology
chemical_list: D015220:Calcium Channels; D015221:Potassium Channels; D016686:Monocrotaline