title: [Mutational analysis of hOGG1 gene promoter in patients with non-small cell lung cancer].
abstract: OBJECTIVE
  8-hydroxygumine DNA glycosylase 1 (OGG1) is a DNA repair enzyme, which can repair damaged DNA by excising 8-dihydro-8-oxoguanine (8-OH-G). Polymorphisms in human OGG1 gene (hOGG1) may alter glycosylase activity, thereby affects its repair to the damaged DNA, resulting in contribution to carcinogenesis. However, an association of genetic variants of hOGG1 promoter with non-small cell lung cancer (NSCLC) remains unclear. The present study aims to explore whether there are mutations in the promoter region of hOGG1 and the association of the potential genetic variants with NSCLC.
  
  
  METHODS
  Forty lung cancer patients were enrolled from January, 2003 to December, 2005 in the first affiliated hospital of Soochow University. PCR-SSCP followed by direct sequencing were performed to detect mutations within the promoter region of the hOGG1 gene in NSCLC and corresponding paracancerous lung tissues.
  
  
  RESULTS
  No abnormal mutation was found in the promoter region of the hOGG1 gene in 40 patients with NSCLC. However, a SNP rs159153 in hOGG1 was significantly associated with higher TNM stage (P=0.008). Moreover, lower frequency of lymph node metastasis was observed in smoker patients with NSCLC (P=0.034).
  
  
  CONCLUSIONS
  The SNP rs159153 in the promoter region of the hOGG1 gene and smoking history may effectively forebode the aggressiveness and metastatic potential of NSCLC.
authors.affiliation: Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Suzhou, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Rengyun
authors.initials: R
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Lei
authors.forename: Jiacong
authors.initials: J
authors.lastname: You
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D045647:DNA Glycosylases / Q000235:genetics* / Q000378:metabolism; D004252:DNA Mutational Analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic
chemical_list: D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human