title: Matrix metalloproteinase 9 levels in gingival crevicular fluid in patients after periodontal microsurgery for orthodontic induced gingival hypertrophy.
abstract: BACKGROUND
  In this study, we aim to compare the levels of matrix metalloproteinase 9 (MMP9) in the gingival crevicular fluid (GCF), as indicators for healing, in two groups of patients - operated with a classic periodontal surgical technique and the same technique but using a dental microscope.
  
  
  METHODS
  We included 14 patients with ages between 12 and 26 years, average 14±6.2 years. Eight patients were women and six men. All patients presented gingival hypertrophy because of the orthodontic treatment on the mandibular arch. We performed gingivectomy on one-half of the mandibular arch by classic periodontal surgery and on the other half of the mandibular arch by a microscope-assisted gingivectomy.
  
  
  METHODS
  In the hypertrophied gingiva, the expression of MMP9 was identified using immunohistochemical-staining techniques. For immunological determination of MMP9 in GCF we performed Elisa tests.
  
  
  RESULTS
  We found different levels in different moments of the healing process for the two hemiarcades.
  
  
  CONCLUSIONS
  We consider that faster healing in case of microscope-assisted gingivectomy may be related to the expression of MMP-9 in the GCF.
authors.affiliation: Department of Orthodontics, University of Medicine and Pharmacy of Craiova, Romania.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Rauten
authors.forename: Petra
authors.initials: P
authors.lastname: Surlin
authors.forename: B
authors.initials: B
authors.lastname: Oprea
authors.forename: Izabela
authors.initials: I
authors.lastname: Siloşi
authors.forename: Mihaela
authors.initials: M
authors.lastname: Moisa
authors.forename: D
authors.initials: D
authors.lastname: Caramizaru
authors.forename: Mihaela
authors.initials: M
authors.lastname: Vătu
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005886:Gingival Hypertrophy / Q000201:enzymology / Q000209:etiology* / Q000473:pathology / Q000601:surgery*; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008866:Microsurgery; D009967:Orthodontic Appliances / Q000009:adverse effects*; D010519:Periodontium / Q000601:surgery*; D055815:Young Adult
chemical_list: D020780:Matrix Metalloproteinase 9