title: Nipkow confocal imaging from deep brain tissues.
abstract: One of the problems in imaging from brain tissues is light-scattering. Thus, multiphoton laser scanning microscopy is widely used to optically access fluorescent signals located deeply in tissues. Here we report that Nipkow-type spinning-disk one-photon confocal microscopy, which embodies high temporal resolution and slow photobleaching, is also capable of imaging tissues to a depth of up to 150 μm. Using a Nipkow-disk microscope, we conducted functional multi-cell calcium imaging of CA3 neurons from in toto intact hippocampal preparations and astrocytes from in vivo neocortical layer 1. This novel application of Nipkow-disk microscopy expands the potential usefulness of this type of microscopy and will contribute to our understanding of natural neuronal microcircuitry.
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahara
authors.forename: Norio
authors.initials: N
authors.lastname: Matsuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
mesh_terms: D000814:Aniline Compounds / Q000032:analysis; D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology; D001921:Brain / Q000166:cytology; D056654:CA3 Region, Hippocampal / Q000737:chemistry / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D019579:Neocortex / Q000737:chemistry / Q000166:cytology*; D017785:Photons; D014966:Xanthenes / Q000032:analysis
chemical_list: D000814:Aniline Compounds; C409648:Fluo 4; D014966:Xanthenes