title: [Establishment of a nasopharyngeal carcinoma cell line CNE1 stably overexpressing ebv-miR-BART7 mediated by lentivirus].
abstract: OBJECTIVE
  To establish a nasopharyngeal carcinoma (NPC) cell line CNE1-pLVTHM/BART7 with stable ebv-miR-BART7 overexpression.
  
  
  METHODS
  The recombinant lentivirus pLVTHM/BART7 expression plasmid was packaged into mature lentivirus by 293FT cells and used to infect CNE1 cells. Flow cytometry was employed for sorting the GFP(+) cells. The efficiency of ebv-miR-BART7 overexpression was determined using qRT-PCR.
  
  
  RESULTS
  The recombinant lentivirus plasmid pLVTHM/BART7 was successfully constructed and verified by PCR and sequencing. The expression of ebv-miR-BART7 in CNE1 cells infected with the lentivirus pLVTHM/BART7 was significantly increased as compared with the negative control and the blank control cells.
  
  
  CONCLUSIONS
  The recombinant lentivirus vector pLVTHM/BART7 results in high and stable expression of ebv-miR-BART7 in infected CNE1 cells, which provides a useful cell model for further studies of the role of ebv-miR-BART7 in nasopharyngeal carcinoma.
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, China. yuancuncun@yahoo.com.cn
authors.forename: Cun-cun
authors.initials: CC
authors.lastname: YUAN
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: CAI
authors.forename: Yu-qi
authors.initials: YQ
authors.lastname: HUANG
authors.forename: Yan-fen
authors.initials: YF
authors.lastname: YE
authors.forename: Man-li
authors.initials: ML
authors.lastname: ZHAO
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: LÜ
authors.forename: Xin
authors.initials: X
authors.lastname: LI
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D035683:MicroRNAs; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D010957:Plasmids
chemical_list: D035683:MicroRNAs