title: Direct interaction of RNA polymerase II and mediator required for transcription in vivo.
abstract: Gene transcription is highly regulated. Altered transcription can lead to cancer or developmental diseases. Mediator, a multisubunit complex conserved among eukaryotes, is generally required for RNA polymerase II (Pol II) transcription. An interaction between the two complexes is known, but its molecular nature and physiological role are unclear. We identify a direct physical interaction between the Rpb3 Pol II subunit of Saccharomyces cerevisiae and the essential Mediator subunit, Med17. Furthermore, we demonstrate a functional element in the Mediator-Pol II interface that is important for genome-wide Pol II recruitment in vivo. Our findings suggest that a direct interaction between Mediator and Pol II is generally required for transcription of class II genes in eukaryotes.
authors.affiliation: Commissariat à l'Energie Atomique et aux Energies Alternatives, iBiTec-S, Service de Biologie Intégrative et Génétique Moléculaire, Gif-sur-Yvette cedex, France. julie.soutourina@cea.fr
authors.forename: Julie
authors.initials: J
authors.lastname: Soutourina
authors.forename: Sandra
authors.initials: S
authors.lastname: Wydau
authors.forename: Yves
authors.initials: Y
authors.lastname: Ambroise
authors.forename: Claire
authors.initials: C
authors.lastname: Boschiero
authors.forename: Michel
authors.initials: M
authors.lastname: Werner
mesh_terms: D047369:Chromatin Immunoprecipitation; D005686:Galactokinase / Q000235:genetics; D005800:Genes, Fungal; D016681:Genome, Fungal; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014158:Transcription, Genetic
chemical_list: D056892:Mediator Complex; C082148:SRB4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase; D012319:RNA Polymerase II; C112110:RPB3 protein, S cerevisiae