title: Detection of Shiga toxin-producing Escherichia coli in meat marketed in Casablanca (Morocco).
abstract: The contamination of meat and meat products with Shiga toxin-producing O157:H7 and non-O157 Escherichia coli (STEC), obtained from markets in Casablanca, Morocco, was investigated. A total of 460 meat and meat products were sampled between March 2004 and July 2006 analysed and 176 strains of E. coli were isolated from these samples. The presence of the stx1, stx2, eae and ehxA genes, recognized as major virulence factors of STEC, was tested in E. coli isolates by polymerase chain reaction (PCR). STEC was detected in 4 (0.9%) samples. The result of serotyping by molecular method showed that two of these STEC isolates corresponded to the serotype O157:H7. The others Shiga toxin-producing E. coli non-O157 corresponded to O6:H21 and O76:H19. The presence of O157:H7 and non-O157 STEC in meat and meat products marketed in Casablanca, Morocco, emphasizes the importance of implementing the Hazard Analysis and Critical Control Point (HACCP) system, as well as the need for implementing, evaluating, and validating antimicrobial interventions to reduce the presence of potential pathogenic microorganisms.
authors.affiliation: Laboratoire de Microbiologie et Hygiène des Aliments et de l'Environnement, Institut Pasteur du Maroc, Casablanca, Morocco. samira_bdm@yahoo.fr
authors.forename: S
authors.initials: S
authors.lastname: Badri
authors.forename: A
authors.initials: A
authors.lastname: Fassouane
authors.forename: I
authors.initials: I
authors.lastname: Filliol
authors.forename: M
authors.initials: M
authors.lastname: Hassar
authors.forename: N
authors.initials: N
authors.lastname: Cohen
mesh_terms: D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005516:Food Microbiology / Q000379:methods; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology*; D009018:Morocco; D016133:Polymerase Chain Reaction / Q000379:methods; D017751:Safety Management / Q000379:methods; D012703:Serotyping / Q000379:methods; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors