title: [Study on the immune response of the therapeutic multi-epitope gene vaccine of hepatitis B virus in mice].
abstract: OBJECTIVE
  To study the immunogenicity of the therapeutic multi-epitope gene vaccine of Hepatitis B virus.
  
  
  METHODS
  Multi-epitope gene of Hepatitis B virus was cloned into prokaryon expression vector pBAD/gIIIA to express a non-fusional antigen B-BPT and BALB/c mice were immunized with the antigen. Cytotoxic T-lymphocyte(CTL) was determined with CytoTox96 kit which quantitatively measures the lactate dehydrogenase(LDH) that is released on cell lysis. CD4(+);, CD8(+); T lymphocytes subsets in immunized mice was detected by using flow cytometry (FCM). Lymphocyte proliferation response was completed by MTT colorimetry.
  
  
  RESULTS
  Injection of B-BPT elicited high-level of CTL response and also stimulus spleen lymphocytes to proliferate effectively.
  
  
  CONCLUSIONS
  B-BPT can induce specific cellular immune responses and may be a good candidate for therapeutic vaccine.
authors.affiliation: Department of Infectious Disease, The 458 Hospital of PLA, Southern Medical University, Guangzhou 510602, China. liminluo@hotmail.com
authors.forename: Li-min
authors.initials: LM
authors.lastname: Luo
authors.forename: Hu
authors.initials: H
authors.lastname: Xia
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects; D018929:Cell Culture Techniques; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors / Q000187:drug effects; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects; D007114:Immunization / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects
chemical_list: D000939:Epitopes; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011994:Recombinant Proteins