title: The effect of Habrobracon hebetor venom on the activity of the prophenoloxidase system, the generation of reactive oxygen species and encapsulation in the haemolymph of Galleria mellonella larvae.
abstract: The cellular and humoral immune reactions in haemolymph of the wax moth Galleria mellonella larvae naturally injected by venom of ectoparasitic wasp Habrobracon hebetor were analyzed. A strong decline of phenoloxidase (PO) activity in the haemolymph and the number of haemocytes with PO activity of envenomated wax moth was observed. In addition, it has been shown that the rate of capsule melanization in the envenomated larvae was half that of the control. Also production of reactive oxygen species (ROS) in the haemolymph of envenomated larvae decreased. The obtained data casts light on the suppression of the main immune reactions in G. mellonella larvae during natural envenomation by H. hebetor.
authors.affiliation: Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Frunze Str., 11, Novosibirsk 630091, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Kryukova
authors.forename: I M
authors.initials: IM
authors.lastname: Dubovskiy
authors.forename: E A
authors.initials: EA
authors.lastname: Chertkova
authors.forename: Ya L
authors.initials: YL
authors.lastname: Vorontsova
authors.forename: I A
authors.initials: IA
authors.lastname: Slepneva
authors.forename: V V
authors.initials: VV
authors.lastname: Glupov
mesh_terms: D000818:Animals; D004156:Catechol Oxidase / Q000276:immunology*; D004792:Enzyme Precursors / Q000276:immunology*; D006458:Hemolymph / Q000201:enzymology / Q000276:immunology; D006790:Host-Parasite Interactions; D019476:Insect Proteins / Q000276:immunology*; D007814:Larva / Q000201:enzymology / Q000276:immunology / Q000469:parasitology; D009036:Moths / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000469:parasitology*; D017382:Reactive Oxygen Species / Q000276:immunology*; D014862:Wasp Venoms / Q000378:metabolism*; D014863:Wasps / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D019476:Insect Proteins; D017382:Reactive Oxygen Species; D014862:Wasp Venoms; C038451:pro-phenoloxidase; D004156:Catechol Oxidase