title: Validated primer set that prevents nuclear DNA sequences of mitochondrial origin co-amplification: a revision based on the New Human Genome Reference Sequence (GRCh37).
abstract: A new human genome reference sequence--GRCh37--was recently generated and made available by the Genome Reference Consortium. Since the prior disposable human reference sequence--hg18--was previously used for the mitochondrial DNA primer BLAST validation, a revision of those previously published primer pairs is required. Thus, the aim of this Short Communication is to perform an in silico BLAST test of the published disposable nine primer pairs using the new human reference sequence and to report the pertinent modifications. The new analysis showed that one of the tested primer pairs requires a revision. Therefore, a new validated primer pair, which specifically amplifies the mitochondrial region located between positions 6520 and 9184, is presented.
authors.affiliation: Unitat d'Antropologia Biològica, Departament BABVE, Universitat Autònoma de Barcelona, Barcelona, Spain. amanda.ramos@uab.cat
authors.forename: Amanda
authors.initials: A
authors.lastname: Ramos
authors.forename: Cristina
authors.initials: C
authors.lastname: Santos
authors.forename: Elena
authors.initials: E
authors.lastname: Barbena
authors.forename: Ligia
authors.initials: L
authors.lastname: Mateiu
authors.forename: Luis
authors.initials: L
authors.lastname: Alvarez
authors.forename: Ramon
authors.initials: R
authors.lastname: Nogués
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Aluja
mesh_terms: D001483:Base Sequence; D003198:Computer Simulation; D017931:DNA Primers / Q000592:standards*; D004272:DNA, Mitochondrial / Q000235:genetics*; D015894:Genome, Human; D023281:Genomics / Q000592:standards*; D006801:Humans; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D012107:Research Design; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000592:standards
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial