title: Coordinated age-dependent and pancreatic-specific expression of mouse Reg2Reg3α, and Reg3β genes.
abstract: Reg family proteins such as Reg1 and islet neogenesis-associated protein (INGAP) have long been implicated in the growth and/or neogenesis of pancreatic islet cells. Recent reports further suggest similar roles to be played by new members such as Reg2, Reg3α, and Reg3β. We have studied their age-, isoform-, and tissue-specific expressions. RNA and protein were isolated from C57BL/6 mice aged 7, 30, and 90 days. Using real-time polymerase chain reaction, the levels of Reg gene expression in the pancreas were 20-600-fold higher than that in other tissues (≫duodenum>stomach>liver); gene expression of Reg2, Reg3α, and Reg3β was age dependent as it was hardly detectable at day 7, increased drastically at day 30, and significantly decreased at day 90; the levels of pancreatic proteins displayed similar age-dependent variations. Using dual-labeled immunofluorescence, Reg2, Reg3α, and Reg3β were abundantly expressed in most acinar cells of the pancreas, in contrast to INGAP which exhibited stepwise increases from day 7 to day 90 and colocalized with the α-cells. These new Reg genes were mainly expressed in the pancreas, with clear age-dependent and isoform-specific patterns.
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Cecile
authors.initials: C
authors.lastname: Jacovetti
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Tehmina
authors.initials: T
authors.lastname: Siddique
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Xiong
authors.forename: Hongping
authors.initials: H
authors.lastname: Yin
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Liu
mesh_terms: D000367:Age Factors; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D020033:Protein Isoforms; D011506:Proteins; C000612141:REG3A protein, human; C490253:Reg3b protein, mouse