title: Development of single-stranded DNA aptamers for specific Bisphenol a detection.
abstract: The development of reagents with high affinity and specificity to small molecules is crucial for the high-throughput detection of chemical compounds, such as toxicants or pollutants. Aptamers are short and single-stranded (ss) oligonucleotides able to recognize target molecules with high affinity. Here, we report the selection of ssDNA aptamers that bind to Bisphenol A (BPA), an environmental hormone. Using SELEX process, we isolated high affinity aptamers to BPA from a 10(15) random library of 60 mer ssDNAs. The selected aptamers bound specifically to BPA, but not to structurally similar molecules, such as Bisphenol B with one methyl group difference, or 4,4'-Bisphenol with 2 methyl groups difference. Using these aptamers, we developed an aptamer-based sol-gel biochip and detected BPA dissolved in water. This novel BPA aptamer-based detection can be further applied to the universal and high-specificity detection of small molecules.
authors.affiliation: Department of Biomedical Engineering, Dongguk University , Seoul, Korea.
authors.forename: Minjoung
authors.initials: M
authors.lastname: Jo
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ahn
authors.forename: Joohyung
authors.initials: J
authors.lastname: Lee
authors.forename: Seram
authors.initials: S
authors.lastname: Lee
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Hong
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Yoo
authors.forename: Jeehye
authors.initials: J
authors.lastname: Kang
authors.forename: Pooja
authors.initials: P
authors.lastname: Dua
authors.forename: Dong-Ki
authors.initials: DK
authors.lastname: Lee
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
authors.forename: Soyoun
authors.initials: S
authors.lastname: Kim
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001559:Benzhydryl Compounds; D001665:Binding Sites / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D036103:Nanotechnology / Q000295:instrumentation; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D012680:Sensitivity and Specificity; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds