title: One hundred consecutive cases of sentinel lymph node mapping in colon cancer-the results of prospective, single--centre feasibility study with implementation of immunohistochemical staining.
abstract: OBJECTIVE
  Although the importance of sentinel node biopsy (SNB) in colon cancer (CC) has not been clearly established, this method is proposed as potentially enabling more appropriate staging by means of immunohistochemistry (IHS). The aim of the study was to evaluate the SNB method used in CC treatment taking into consideration the results of the IHS examination.
  
  
  METHODS
  In the period from May 2005 to September 2010 in the 1st Department of Surgical Oncology and General Surgery, Wielkopolska Cancer Centre, 100 SNB in CC were performed. Sentinel nodes (SN) were identified intraoperatively with the use of Patent Blue dye. In the case of negative hematoxylin and eosin staining, the SN material was subjected to immunohistochemical examination. Finally, the histopathological findings of sentinel and non-sentinel lymph nodes were compared with the results of the immunohistochemical staining.
  
  
  RESULTS
  At least one SN was identified in 99 of 100 patients (99%). The SN was the only place of metastases in 12.1% (12/99) of the patients. The accuracy of SNB in determining the regional lymph node status was 93.9% (93/99). The sensitivity of the method was 83.3% (30/36). The false-negative rate amounted to 16.7% (6/36). Upstaging obtained by the implementation of the immunohistochemical method was 10% (7/70).
  
  
  CONCLUSIONS
  The application of the immunohistochemical staining enables upstaging of some patients, potentially benefiting from adjuvant chemotherapy. For full and definitive assessment of SNB in CC, further research is required especially in terms of additional factors determining a patient's eligibility for this procedure.
authors.affiliation: 1st Clinic of Surgical Oncology and General Surgery, Wielkopolska Cancer Centre, Garbary Street 15, 61-866, Poznań, Poland. dmurawa@o2.pl
authors.forename: Dawid
authors.initials: D
authors.lastname: Murawa
authors.forename: Piotr
authors.initials: P
authors.lastname: Nowaczyk
authors.forename: Michael
authors.initials: M
authors.lastname: Hünerbein
authors.forename: Karol
authors.initials: K
authors.lastname: Połom
authors.forename: Violetta
authors.initials: V
authors.lastname: Filas
authors.forename: Jan
authors.initials: J
authors.lastname: Bręborowicz
authors.forename: Paweł
authors.initials: P
authors.lastname: Murawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000473:pathology* / Q000601:surgery*; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011446:Prospective Studies; D021701:Sentinel Lymph Node Biopsy