title: [Genetic diversity of Legionella pneumophila serogroup 1 in hospital water distribution systems in Shanghai].
abstract: OBJECTIVE
  To classify Legionella pneumophila serogroup 1 (LP1) isolated from the water distribution systems in hospitals of Shanghai by using genotying methods, and therefore to explore the genetic relationship between different genotypes.
  
  
  METHODS
  LP1 was isolated from 8 hospitals in Shanghai, and strains of LP1 were subtyped using pulse field gel electrophoresis (PFGE). Genotyping results of PFGE were classified as dendrogram, and cluster analysis was used to compare the genetic characteristics of different strains.
  
  
  RESULTS
  Twenty-four strains of LP1 were isolated from 193 water specimens. LP1 belonged to 6 PFGE genotypes, of which 21 belonged to 3 predominant genotypes. Different hospitals shared the same genotype, while some strains isolated from the same hospital had different genotypes.
  
  
  CONCLUSIONS
  The genetic characteristics are stable in environmental LP1. Molecular biology techniques should be added to epidemiological study for hospital acquired legionellosis due to the genetic diversity in one hospital.
authors.affiliation: Zhongshan Hospital Affiliated of Fudan University, Shanghai 200032, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Tao
authors.forename: Bi-Jie
authors.initials: BJ
authors.lastname: Hu
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yu
authors.forename: Zhao-Yan
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Xie
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhou
mesh_terms: D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field; D004865:Equipment and Supplies, Hospital / Q000382:microbiology*; D005838:Genotype; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D014871:Water Microbiology; D014881:Water Supply