title: Detection of seasonal H3N2 influenza A virus by type-specific TaqMan minor groove binder probe assay.
abstract: Despite the emergence of the pandemic H1N1 influenza A virus in 2009, seasonal H3N2 viruses continue to co-circulate in the population and may even predominate in the coming influenza season. We describe a specific minor groove binder TaqMan assay for H3N2 viruses with a detection limit of 16.5 standard DNA copies.
authors.affiliation: Viral Pathogenesis and Evolution Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Wang
authors.forename: Louis M
authors.initials: LM
authors.lastname: Schwartzman
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Memoli
authors.forename: Jeffery K
authors.initials: JK
authors.lastname: Taubenberger
mesh_terms: D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D015345:Oligonucleotide Probes; D012367:RNA, Viral