title: A novel gene cassette, aacA43, in a plasmid-borne class 1 integron.
abstract: A novel gene cassette, aacA43, was identified in the aadB-aacA43-oxa10-smr2 cassette array in a class 1 integron. Like related aminoglycoside-(6')-acetyltransferases, AacA43 confers clinically relevant resistance to kanamycin, tobramycin, and some less-used aminoglycosides but not to gentamicin. Although transferable on an IncL/M plasmid, aacA43 was identified in only two different Klebsiella pneumoniae strains (14 isolates), one Escherichia coli strain (2 isolates), and one Enterobacter cloacae strain in a survey of patients in a Sydney intensive care unit in 2004-2005.
authors.affiliation: Centre for Infectious Diseases and Microbiology, Level 3, ICPMR Building, Westmead Hospital, Wentworthville, New South Wales 2145, Australia. sally.partridge@swahs.health.nsw.gov.au
authors.forename: Sally R
authors.initials: SR
authors.lastname: Partridge
authors.forename: Lee C
authors.initials: LC
authors.lastname: Thomas
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Ginn
authors.forename: Agnieszka M
authors.initials: AM
authors.lastname: Wiklendt
authors.forename: Pierre
authors.initials: P
authors.lastname: Kyme
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Iredell
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D016972:Enterobacter cloacae / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D032023:Integrons; D007711:Klebsiella pneumoniae / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction
chemical_list: D000123:Acetyltransferases