title: Bryophyte DNA sequences from faeces of an arctic herbivore, barnacle goose (Branta leucopsis).
abstract: We tested DNA extraction methods and PCR conditions for the amplification of bryophyte DNA from barnacle goose (Branta leucopsis) faeces collected from Spitsbergen (Svalbard). Both the Qiagen stool kit and a silica-based extraction method received sufficient DNA from fresh and older droppings, as indicated by successful amplification of the plastid psbA-trnH spacer. Standard Taq polymerase outperformed two hot start polymerases. Sequencing of cloned PCR products revealed at least ten moss and two angiosperm sequences. This first example of identifying bryophyte DNA from faeces will allow analysing moss diets of arctic herbivores with a DNA barcoding approach.
authors.affiliation: Netherlands Centre for Biodiversity Naturalis, Section Nationaal Herbarium Nederland, Leiden University, Leiden, The Netherlands. stech@nhn.leidenuniv.nl
authors.forename: M
authors.initials: M
authors.lastname: Stech
authors.forename: E
authors.initials: E
authors.lastname: Kolvoort
authors.forename: M J J E
authors.initials: MJ
authors.lastname: Loonen
authors.forename: K
authors.initials: K
authors.lastname: Vrieling
authors.forename: J D
authors.initials: JD
authors.lastname: Kruijer
mesh_terms: D000818:Animals; D001110:Arctic Regions; D044002:Bryophyta / Q000145:classification* / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant / Q000235:genetics; D004435:Eating; D005243:Feces / Q000737:chemistry*; D005777:Geese / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D018744:DNA, Plant