title: Rapid immobilization of enzymes onto solid supports by ultrasound waves.
abstract: Herein, we describe a non-conventional method for immobilization of enzymes onto different solid surfaces using ultrasound as a source of energy. When horseradish peroxidase (HRP) was taken on the surface of an activated support and allowed to float on a sonicator bath operating at a frequency of 40 KHz, it readily started binding itself to the surface. Maximum binding was observed in 10 min whereas a control experiment carried out similarly without ultrasound waves showed insignificant immobilization. Ultrasound wave-mediated immobilization is rapid and reproducible and is better suitable for versatile applications in different fields, including fabrication of enzyme-based biosensors or bioreactors.
authors.affiliation: Institute of Genomics and Integrative Biology (CSIR), Delhi, India.
authors.forename: Pragya
authors.initials: P
authors.lastname: Sharma
authors.forename: Dileep Kumar
authors.initials: DK
authors.lastname: Kannoujia
authors.forename: Seemi Farhat
authors.initials: SF
authors.lastname: Basir
authors.forename: Pradip
authors.initials: P
authors.lastname: Nahar
mesh_terms: D001386:Azides / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D003124:Colorimetry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D019220:High-Energy Shock Waves; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D009578:Nitrobenzenes / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011485:Protein Binding; D013010:Sonication / Q000379:methods*
chemical_list: C492759:1-fluoro-2-nitro-4-azidobenzene; D001386:Azides; D004800:Enzymes, Immobilized; D009578:Nitrobenzenes; D011137:Polystyrenes; D006735:Horseradish Peroxidase