title: Spatially quantifying microscopic tumor invasion and proliferation using a voxel-wise solution to a glioma growth model and serial diffusion MRI.
abstract: The purpose of this study was to develop a voxel-wise analytical solution to a glioma growth model using serial diffusion MRI. These cell invasion, motility, and proliferation level estimates (CIMPLE maps) provide quantitative estimates of microscopic tumor growth dynamics. After an analytical solution was found, noise simulations were performed to predict the effects that perturbations in apparent diffusion coefficient values and the time between apparent diffusion coefficient map acquisitions would have on the accuracy of CIMPLE maps. CIMPLE maps were then created for 53 patients with gliomas with WHO grades of II-IV. MR spectroscopy estimates of the choline-to-N-acetylaspartate ratio were compared to cell proliferation estimates in CIMPLE maps using Pearson's correlation analysis. Median differences in cell proliferation and diffusion rates between WHO grades were compared. A strong correlation (R(2) = 0.9714) and good spatial correspondence were observed between MR spectroscopy measurements of the choline-to-N-acetylaspartate ratio and CIMPLE map cell proliferation rate estimates. Estimates of cell proliferation and diffusion rates appear to be significantly different between low- (WHO II) and high-grade (WHO III-IV) gliomas. Cell diffusion rate (motility) estimates are highly dependent on the time interval between apparent diffusion coefficient map acquisitions, whereas cell proliferation rate estimates are additionally influenced by the level of noise present in apparent diffusion coefficient maps.
authors.affiliation: Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Ellingson
authors.forename: Peter S
authors.initials: PS
authors.lastname: LaViolette
authors.forename: Scott D
authors.initials: SD
authors.lastname: Rand
authors.forename: Mark G
authors.initials: MG
authors.lastname: Malkin
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Connelly
authors.forename: Wade M
authors.initials: WM
authors.lastname: Mueller
authors.forename: Robert W
authors.initials: RW
authors.lastname: Prost
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Schmainda
mesh_terms: D000465:Algorithms; D001932:Brain Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D049109:Cell Proliferation; D003198:Computer Simulation; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D005909:Glioblastoma / Q000473:pathology* / Q000503:physiopathology*; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods; D008954:Models, Biological; D009361:Neoplasm Invasiveness; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity