title: DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum.
abstract: Mitochondrial DNA (mtDNA) is generally packaged into the mitochondrial nucleoid (mt-nucleoid) by a high-mobility group (HMG) protein. Glom is an mtDNA-packaging HMG protein in Physarum polycephalum. Here we identified a new mtDNA-packaging protein, Glom2, which had a region homologous with yeast Mgm101. Glom2 could bind to an entire mtDNA and worked synergistically with Glom for condensation of mtDNA in vitro. Down-regulation of Glom2 enhanced the alteration of mt-nucleoid morphology and the loss of mtDNA induced by down-regulation of Glom, and impaired mRNA accumulation of some mtDNA-encoded genes. These data suggest that Glom2 may organize the mt-nucleoid coordinately with Glom.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan. kie@gene.nagoya-u.ac.jp
authors.forename: Kie
authors.initials: K
authors.lastname: Itoh
authors.forename: Akiko
authors.initials: A
authors.lastname: Izumi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Mori
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Ryoko
authors.initials: R
authors.lastname: Yui
authors.forename: Katsura
authors.initials: K
authors.lastname: Maeda-Sano
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shirai
authors.forename: Masahiro M
authors.initials: MM
authors.lastname: Kanaoka
authors.forename: Tsuneyoshi
authors.initials: T
authors.lastname: Kuroiwa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higashiyama
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sugita
authors.forename: Kimiko
authors.initials: K
authors.lastname: Murakami-Murofushi
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawano
authors.forename: Narie
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D042003:DNA Packaging; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D006609:High Mobility Group Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016839:Physarum polycephalum / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006609:High Mobility Group Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins