title: Dietary glucosylceramide enhances cornified envelope formation via transglutaminase expression and involucrin production.
abstract: In this study, we investigated whether dietary glucosylceramide (GlcCer) and its metabolite sphingoid bases, sphingosine (SS), phytosphingosine (PS), sphingadienine (SD) and 4-hydroxysphingenine (4HS), influence cornified envelope (CE) formation. CE is formed during terminal differentiation of the epidermis through crosslinking of specific precursor proteins by transglutaminases (TGases), and is essential for the skin's barrier function. Oral administration of GlcCer (0.25 mg/day) for 14 consecutive days dramatically reduced transepidermal water loss, an indicator of the skin barrier condition, in hairless mice with barrier perturbation induced by single-dose ultraviolet B (UVB) irradiation. The GlcCer treatment also increased the level of TGase-1 mRNA in UVB-irradiated murine epidermis approximately 1.6-fold compared with the control. Further, all four sphingoid bases at 1 μM concentration enhanced CE formation of cultured normal human keratinocyte cells. Among them, SS, PS and SD, but not 4HS, stimulated production of involucrin, one of the CE major precursor proteins. SD increased the expression of TGase-1 mRNA, while SS increased the expression of TGase-3 mRNA. These results indicate that the skin barrier improvement induced by oral GlcCer treatment might be at least partly due to a reinforcement of CE formation in the epidermis mediated by sphingoid bases metabolically derived from GlcCer.
authors.affiliation: Shiseido Functional Food Research and Development Center, 2-12-1 Fukuura, Kanazawa-ku, Yokohama 236-8643, Japan. tatsuya.hasegawa@to.shiseido.co.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Shimada
authors.forename: Taro
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Osamu
authors.initials: O
authors.lastname: Ueda
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakashima
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Matsuoka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005963:Glucosylceramides / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D011498:Protein Precursors / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D011503:Transglutaminases / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D005963:Glucosylceramides; D011498:Protein Precursors; C032253:involucrin; D011503:Transglutaminases