title: Receptor for advanced glycation end products binds to phosphatidylserine and assists in the clearance of apoptotic cells.
abstract: Clearance of apoptotic cells is necessary for tissue development, homeostasis and resolution of inflammation. The uptake of apoptotic cells is initiated by an 'eat-me' signal, such as phosphatidylserine, on the cell surface and phagocytes recognize the signal by using specific receptors. In this study, we show that the soluble form of the receptor for advanced glycation end products (RAGE) binds to phosphatidylserine as well as to the apoptotic thymocytes. RAGE-deficient (Rage(-/-)) alveolar macrophages showed impaired phagocytosis of apoptotic thymocytes and defective clearance of apoptotic neutrophils in Rage(-/-) mice. Our results indicate that RAGE functions as a phosphatidylserine receptor and assists in the clearance of apoptotic cells.
authors.affiliation: Department of Advanced Preventive Medicine for Infectious Disease, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan.
authors.forename: Mei
authors.initials: M
authors.lastname: He
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kubo
authors.forename: Konosuke
authors.initials: K
authors.lastname: Morimoto
authors.forename: Naoya
authors.initials: N
authors.lastname: Fujino
authors.forename: Takaya
authors.initials: T
authors.lastname: Suzuki
authors.forename: Toru
authors.initials: T
authors.lastname: Takahasi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yamada
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Yamaya
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Maekawa
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D018613:Microscopy, Confocal; D010587:Phagocytosis / Q000235:genetics / Q000502:physiology*; D010718:Phosphatidylserines / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D010718:Phosphatidylserines; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic