title: [The effect of ginsenoside Rb1 on phagocytosis, the production of cytokines and NO of murine macrophage in vitro].
abstract: OBJECTIVE
  Investigate the effects of Rb1 on phagocytosis and the production of the pro-inflammatory cytokines and NO of murine macrophage, to elucidate the machanism of the immuno-regulating effect of Rb1.
  
  
  METHODS
  The single cell suspension of murine peritoneal macrophages was prepared under sterile condition. Then, the drug toxicology of different concent rations of Rb1 on macrophages was measured by MTT assay. The phagocytosis of macrophages to microbeads was observed through flow cytometry(FCM). The amount of NO produced in macrophages was detected by Griess kit. Used Cytometric Bead Arry(CBA) kit to detect the production of cytokines of Macrophages stimulated by LPS.
  
  
  RESULTS
  In a dose-dependent manner, Rb1(5, 10, 20 μmol/L) can significantly inhibit the phagocytose of macrophage stimulated by LPS, but improve this ability of unactivated macrophage (P<0.05). Rb1 can also reduce the production of NO by macrophage(P<0.01). Rb1 can regulate the production of cytokines of macrophage stimulated by LPS.
  
  
  CONCLUSIONS
  Rb1 can inhibit the transduction of LPS signals and regulate the content of cytokines, thus to decrease the production of NO. However, to the unactivated macrophages, Rb1 up-regulates phagocytic functions and promotes the innate immunity to resist invasion of pathogens.
authors.affiliation: Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. lvmengjie.0628@163.com
authors.forename: Meng-jie
authors.initials: MJ
authors.lastname: Lu
authors.forename: Yao-ying
authors.initials: YY
authors.lastname: Zeng
authors.forename: Bing
authors.initials: B
authors.lastname: Song
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis*; D010587:Phagocytosis / Q000187:drug effects*
chemical_list: D016207:Cytokines; D036145:Ginsenosides; D009569:Nitric Oxide; C442759:ginsenoside Rb1