title: Platelet and monocyte activity markers and mediators of inflammation in Takotsubo cardiomyopathy.
abstract: Patients with Takotsubo cardiomyopathy (TC) often present with symptoms similar to those of myocardial infarction (MI). We analyzed blood concentrations of mediators of inflammation and platelet- and monocyte-activity markers in patients with TC and MI for significant differences. Clinical data of patients with TC (n = 16) and acute MI (n = 16) were obtained. Serial blood samples were taken at the time of hospital admission (t(0)), after 2-4 days (t(1)) and after 4-7 weeks (t(2)), respectively. Plasma concentrations of interleukin (IL)-6, IL-7, soluble CD40 ligand (sCD40L), and monocyte chemotactic protein 1 (MCP-1) were determined with an ELISA. Tissue factor binding on monocytes, platelet-activation marker CD62P, platelet CD40-ligand (CD40L), and platelet-monocyte aggregates were measured using flow cytometry. Expression of CD62P on platelets and IL-6 plasma levels were significantly lower in patients with TC compared to MI at the time of hospital admission. IL-7 plasma levels were significantly elevated in patients with TC compared to patients with MI at 2-4 days after hospital admission. No significant differences were observed concerning sCD40L and MCP-1 plasma levels, tissue factor binding on monocytes, CD40L expression on platelets, and platelet-monocyte aggregates at any point in time. Our results indicate that inflammatory mediators and platelet-activity markers contribute to the differences in the pathogenesis of MI and TC.
authors.affiliation: 1st Department of Medicine (Cardiology), University Medical Centre Mannheim, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Pirzer
authors.forename: Elif
authors.initials: E
authors.lastname: Elmas
authors.forename: Dariusch
authors.initials: D
authors.lastname: Haghi
authors.forename: Christiane
authors.initials: C
authors.lastname: Lippert
authors.forename: Stefan
authors.initials: S
authors.lastname: Kralev
authors.forename: Siegfried
authors.initials: S
authors.lastname: Lang
authors.forename: Martin
authors.initials: M
authors.lastname: Borggrefe
authors.forename: Thorsten
authors.initials: T
authors.lastname: Kälsch
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000276:immunology*; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005858:Germany; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D009203:Myocardial Infarction / Q000097:blood / Q000276:immunology*; D010343:Patient Admission; D015539:Platelet Activation; D011446:Prospective Studies; D054549:Takotsubo Cardiomyopathy / Q000097:blood / Q000276:immunology*; D013927:Thrombosis / Q000097:blood / Q000276:immunology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators