title: Calcium protects roots of Sedum alfredii H. against cadmium-induced oxidative stress.
abstract: Sedum alfredii is a well-known Cd (cadmium) hyperaccumulator native to China. The impacts of exogenous Ca on Cd-induced oxidative stress and antioxidant systems in roots of S. alfredii were investigated by using cellular and biochemical approaches. Supplementation of the medium with higher Ca levels resulted in alleviated growth inhibition and decreased Cd concentration, as well as increased Ca concentration in roots. Cadmium induced lipid peroxidation and loss of plasma membrane integrity, reactive oxygen species overproduction, as well as ultrastructural changes of root cells were largely reversed by Ca supplementation in the medium. Calcium application significantly altered the Cd effects on antioxidant enzymes and non-enzyme antioxidants (non-protein thiols), and significantly increased glutathione (GSH) biosynthesis. The results suggest that Ca is able to protect the roots of S. alfredii against Cd toxicity by restoration of Cd-displaced Ca, alleviation of the metal induced oxidative stress, as well as promotion of GSH biosynthesis.
authors.affiliation: MOE Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China.
authors.forename: Shengke
authors.initials: S
authors.lastname: Tian
authors.forename: Lingli
authors.initials: L
authors.lastname: Lu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Patrick
authors.initials: P
authors.lastname: Brown
authors.forename: Zhenli
authors.initials: Z
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Yang
mesh_terms: D002104:Cadmium / Q000737:chemistry / Q000633:toxicity*; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D031963:Sedum / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D012987:Soil; D012989:Soil Pollutants; D002104:Cadmium; C121048:guaiacol peroxidase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D002118:Calcium