title: Persicaria hydropiper (L.) spach and its flavonoid components, isoquercitrin and isorhamnetin, activate the Wnt/β-catenin pathway and inhibit adipocyte differentiation of 3T3-L1 cells.
abstract: Obesity, which is related to metabolic syndrome and is associated with liver disease, represents an epidemic problem demanding effective therapeutic strategies. Evidence shows that the Wnt/β-catenin pathway is closely associated with obesity and that small molecules regulating the Wnt/β-catenin pathway can potentially control adipogenesis related to obesity. Eleven plant extracts activating the Wnt/β-catenin pathway were screened by using HEK 293-TOP cells retaining the Wnt/β-catenin signaling reporter gene. An extract of Persicaria hydropiper (L.) Spach was found to activate Wnt/β-catenin signaling. P. hydropiper is grown worldwide in temperate climates and is found widely in Southeast Asia. The P. hydropiper extract inhibited the differentiation of adipocyte 3T3-L1 cells. Isoquercitrin and isorhamnetin, constituents of P. hydropiper, also activated Wnt/β-catenin signaling and suppressed the differentiation of 3T3-L1 cells. These results indicate that isoquercitrin in P. hydropiper suppresses the adipogenesis of 3T3-L1 cells via the inhibition of Wnt/β-catenin signaling. P. hydropiper and isoquercitrin may therefore be potential therapeutic agents for obesity and its associated disorders.
authors.affiliation: Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
authors.forename: Soung-Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Bora
authors.initials: B
authors.lastname: Kim
authors.forename: Myoung Jin
authors.initials: MJ
authors.lastname: Oh
authors.forename: Juyong
authors.initials: J
authors.lastname: Yoon
authors.forename: Hyun Yi
authors.initials: HY
authors.lastname: Kim
authors.forename: Kye Jong
authors.initials: KJ
authors.lastname: Lee
authors.forename: Joo Dong
authors.initials: JD
authors.lastname: Lee
authors.forename: Kang-Yell
authors.initials: KY
authors.lastname: Choi
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D044948:Flavonols / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D019664:Polygonaceae / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D044948:Flavonols; D051153:Wnt Proteins; D051176:beta Catenin; C047368:3-methylquercetin; C016527:isoquercitrin; D011794:Quercetin