title: Determination of isometamidium residues in animal-derived foods by liquid chromatography-tandem mass spectrometry.
abstract: In this paper, a new determination method for isometamidium residues in animal-derived foods was developed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Isometamidium residues in bovine tissues and milk were extracted with the mixed solution of acetonitrile and 0.25 mol/L of ammonium formate-methanol (v/v, 1:1), concentrated and degreased, and determined by LC-MS/MS with quantification by external standard method. The results showed that the peak area of chromatogram was linearly related to the concentration of isometamidium in the range of 1-100 μg/L, and the limits of detection (LOD) and quantification (LOQ) were 0.05 μg/kg and 5 μg/kg, respectively. The average recoveries of spiked samples were in the range of 73.8-93.9% with relative standard deviations ranged from 2.3% to 7.5%. This method is simple, accurate and suitable for the identification and quantification for isometamidium in animal-derived foods.
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Zou
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: C X
authors.initials: CX
authors.lastname: Xi
authors.forename: G M
authors.initials: GM
authors.lastname: Wang
authors.forename: X L
authors.initials: XL
authors.lastname: Li
authors.forename: J Z
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Z G
authors.initials: ZG
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005561:Formates; D016014:Linear Models; D008099:Liver; D008460:Meat / Q000032:analysis*; D000432:Methanol; D008892:Milk / Q000737:chemistry*; D010617:Phenanthridines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014344:Trypanocidal Agents / Q000032:analysis / Q000737:chemistry
chemical_list: D005561:Formates; D010617:Phenanthridines; D014344:Trypanocidal Agents; C030544:formic acid; C000702:isometamidium chloride; D000432:Methanol