title: [Scanning of c-kit gene mutations in acute myeloid leukemias using high-resolution melting analysis].
abstract: OBJECTIVE
  To detect the common mutations (D816V and N822K) of c-kit gene in acute myeloid leukemia (AML) using high-resolution melting analysis (HRM).
  
  
  METHODS
  HRM analysis was established to screen c-kit mutations in PCR products of c-kit exon 17 in 21 AML patients with t(8;21). PCR products were sequenced to confirm the mutation.
  
  
  RESULTS
  HRM analysis identified an aberrant melting curve in 6 cases (28.6%), which were confirmed by direct DNA sequencing as one D816V mutation and five N822K mutation.
  
  
  CONCLUSIONS
  HRM analysis is a convenient, rapid, specific and high-throughput technique for scanning c-kit gene mutation in AML.
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China.
authors.forename: Dong-ming
authors.initials: DM
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Gao-fei
authors.initials: GF
authors.lastname: Xiao
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qian
authors.forename: Run-bi
authors.initials: RB
authors.lastname: Ji
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D004252:DNA Mutational Analysis / Q000379:methods*; D005091:Exons; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D055815:Young Adult
chemical_list: D019009:Proto-Oncogene Proteins c-kit