title: Insights into physiological and genetic mupirocin susceptibility in bifidobacteria.
abstract: Mupirocin is an antibiotic commonly used in selective media for the isolation of bifidobacteria. However, little is known about the genetic traits responsible for bifidobacterial resistance to mupirocin. Our investigation demonstrates that all of the bifidobacteria tested exhibit a phenotype of generally high resistance to this antibiotic. The genotypic reason for bifidobacterial mupirocin resistance was further characterized by sequencing of the isoleucyl-tRNA synthetase gene (ileS) coupled with three-dimensional modeling of the encoded protein and cloning of the ileS gene of Bifidobacterium bifidum PRL2010 in a mupirocin-sensitive Escherichia coli strain. These analyses revealed key amino acid residues of the IleS protein that apparently are crucial for conferring a mupirocin resistance phenotype to bifidobacteria.
authors.affiliation: Department of Genetics, Biology of Microorganisms, Anthropology, and Evolution, University of Parma, Parco Area delle Scienze 11a, 43124 Parma, Italy.
authors.forename: Fausta
authors.initials: F
authors.lastname: Serafini
authors.forename: Francesca
authors.initials: F
authors.lastname: Bottacini
authors.forename: Alice
authors.initials: A
authors.lastname: Viappiani
authors.forename: Enrico
authors.initials: E
authors.lastname: Baruffini
authors.forename: Francesca
authors.initials: F
authors.lastname: Turroni
authors.forename: Elena
authors.initials: E
authors.lastname: Foroni
authors.forename: Tiziana
authors.initials: T
authors.lastname: Lodi
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
authors.forename: Marco
authors.initials: M
authors.lastname: Ventura
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects* / Q000235:genetics; D024881:Drug Resistance, Bacterial; D007533:Isoleucine-tRNA Ligase / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016712:Mupirocin / Q000494:pharmacology*; D011487:Protein Conformation; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D016712:Mupirocin; D007533:Isoleucine-tRNA Ligase