title: Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence polarization.
abstract: We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0×10(-4) U mL(-1) with a detection range from 5.0×10(-4) to 10 U mL(-1).
authors.affiliation: Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.forename: Zhen-Feng
authors.initials: ZF
authors.lastname: Chen
authors.forename: Yan-Cheng
authors.initials: YC
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liang
mesh_terms: D015246:Deoxyribonuclease EcoRI / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005454:Fluorescence Polarization; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D006046:Gold; D015246:Deoxyribonuclease EcoRI