title: Immunohistochemical detection of phosphatidylserine and thrombospondin on denucleating erythroblasts in rat bone marrow.
abstract: The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event.
authors.affiliation: Department of Bioresource Science, Graduate School of Agricultural Science, Graduate School of Science and Technology, Kobe University, Kobe, Japan. tyokoyama@port.kobe-u.ac.jp
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Takahiko
authors.initials: T
authors.lastname: Etoh
authors.forename: Kankanam Gamage Sanath
authors.initials: KG
authors.lastname: Udayanga
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Takahara
authors.forename: Wang-Mei
authors.initials: WM
authors.lastname: Qi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Warita
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hoshi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagawa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D004900:Erythroblasts / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D010718:Phosphatidylserines / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019699:Thrombospondins / Q000378:metabolism*
chemical_list: D010718:Phosphatidylserines; D019699:Thrombospondins