title: Association between HLA-E *0101 homozygosity and recurrent miscarriage in Egyptian women.
abstract: The objective was to investigate the frequency of human leucocyte antigen (HLA)-E alleles in Egyptian women with and without recurrent miscarriage (RM) to evaluate their role on the maintenance of pregnancy. A case-control study was adopted. HLA-E gene polymorphism typing was carried out by restriction fragment length polymorphism for 108 women with RM and 120 fertile female controls. The frequency of HLA-E *0101 allele was higher in patients with RM and HLA-E*0103 allele was higher in fertile controls, and the difference was statistically significant (P=0.003, P(c)=0.006). HLA-E*0101/0101 genotype was the most frequent genotype in patients (45.4%), followed by HLA-E*0101/0103 (44.4%) and finally HLA-E*0103/0103 genotype (10.2%). The difference in the frequency of HLA-E*0101/0101 homozygous genotype in patients with RM compared with that in the fertile controls was statistically significant (OR=2.02, 95% CI=1.13-3.62, P=0.011, P(c)=0.033). We found an increased frequency of homozygosity for HLA-E*0101 in Egyptian women with RM. HLA-E*0101 homozygosity may thus be a risk factor for RM.
authors.affiliation: Mansoura Faculty of Medicine, Clinical Pathology Department, Clinical Immunology Unit, Mansoura, Egypt. youssefmosaad@yahoo.com
authors.forename: Y M
authors.initials: YM
authors.lastname: Mosaad
authors.forename: Y
authors.initials: Y
authors.lastname: Abdel-Dayem
authors.forename: B S
authors.initials: BS
authors.lastname: El-Deek
authors.forename: S M
authors.initials: SM
authors.lastname: El-Sherbini
mesh_terms: D000026:Abortion, Habitual / Q000453:epidemiology / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D004534:Egypt / Q000453:epidemiology; D005260:Female; D056726:Genetic Association Studies / Q000706:statistics & numerical data; D020022:Genetic Predisposition to Disease; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D006720:Homozygote; D006801:Humans; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012307:Risk Factors; D055815:Young Adult; D000097625:HLA-E Antigens
chemical_list: D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens