title: Use of recombinant proteins MPB70 or MPB83 as capture antigens in ELISAs to confirm bovine tuberculosis infections in Brazil.
abstract: The objective was to evaluate the use of two indirect IgG-ELISA tests (with recombinant proteins MPB70 or MPB83, respectively, as capture antigens) as confirmatory tests for diagnosis of bovine tuberculosis in a herd of naturally infected dairy cows. Results for ELISA-MPB70 and ELISA-MPB83 were similar (kappa statistic=0.92) on Days 0 (day of intradermal injection with purified protein derivatives, PPD), 7, and 21. The kappa statistic between ELISA and the Comparative Intradermal Tuberculin Test, as well as ELISA sensitivity and specificity (relative to culture or PCR as standards) were: 0.7, 34.4% and 75% on Day 0; 0.25, 53.8% and 66.6% on Day 7; and 0.01, 1.8% and 77.7% on Day 21, respectively. In conclusion, although ELISAs using MPB70 or MPB83 as antigens were not reliable indicators of infection status, especially on Days 7 and 21, they were of potential value as complementary tools to intradermal PPD testing.
authors.affiliation: Veterinary Bacteriology Laboratory, Universidade Federal Fluminense, R. Hernani Mello, Niterói-RJ, Brazil.
authors.forename: C D
authors.initials: CD
authors.lastname: Marassi
authors.forename: L
authors.initials: L
authors.lastname: Medeiros
authors.forename: J
authors.initials: J
authors.lastname: McNair
authors.forename: W
authors.initials: W
authors.lastname: Lilenbaum
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil; D002417:Cattle; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D008565:Membrane Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014374:Tuberculin Test; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C094904:MPB83 protein, Mycobacterium; D008565:Membrane Proteins; D011994:Recombinant Proteins; C060216:MPB70 protein, Mycobacterium bovis