title: NT-3-secreting human umbilical cord mesenchymal stromal cell transplantation for the treatment of acute spinal cord injury in rats.
abstract: An animal model for clip spinal cord injury (SCI) was used to determine whether Neurotrophin-3 (NT-3) genetically modified human umbilical mesenchymal stem cells (NT-3-HUMSCs) could promote the morphologic and functional recovery of injured spinal cords. Using the Basso, Beattie, and Bresnahan scores and a grid test, the rats in the HUMSC-treated and NT-3-HUMSCs groups had significantly improved locomotor functional recovery more than the control group. In comparison, the NT-3-HUMSCs group achieved better functional recovery than the HUMSCs group at the end of 12 weeks after SCI. The functional recovery was accompanied by increased intensity of 5-HT fibers, increased volume of spared myelination, and decreased area of the cystic cavity in the NT-3-HUMSCs group compared with the HUMSCs group. Moreover, transplanted NT-3-HUMSCs survived and produced larger amounts of NT-3 than the HUMSCs in the host spinal cord. These results show that NT-3 enhanced the therapeutic effects of HUMSCs after clip injury of the spinal cord.
authors.affiliation: Department of Neurosurgery, General Hospital of PLA, Beijing, 100853, China.
authors.forename: Ai-Jia
authors.initials: AJ
authors.lastname: Shang
authors.forename: Sun-Quan
authors.initials: SQ
authors.lastname: Hong
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xu
authors.forename: Hang-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhong-Feng
authors.initials: ZF
authors.lastname: Wang
authors.forename: Bai-Nan
authors.initials: BN
authors.lastname: Xu
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Jiang
authors.forename: Ru-Xiang
authors.initials: RX
authors.lastname: Xu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008124:Locomotion / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009186:Myelin Sheath / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology; D020933:Neurotrophin 3 / Q000096:biosynthesis / Q000378:metabolism*; D011597:Psychomotor Performance / Q000502:physiology; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000601:surgery*; D014161:Transduction, Genetic / Q000379:methods; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D012701:Serotonin; D014446:Tyrosine 3-Monooxygenase