title: Glycoside hydrolases in Aplysia fasciata: analysis and applications.
abstract: Glycosylation is considered to be an important reaction for the chemical modification of compounds with useful biological activities. Glycoside hydrolases are biotechnologically attractive enzymes which can be used in synthetic reactions for assembling glycosidic linkages with absolute stereoselectivity at an anomeric centre. Most of these enzymes are commercially available but there is great interest in the search for new biocatalysts with original catalytic characteristics. The marine environment has shown to be a very interesting source for new glycosyl hydrolases for both hydrolytic and synthetic aspects. In particular, Aplysia fasciata a marine herbivorous mollusc has been shown to be a potent producer of a library of glycoside hydrolases applied to the synthesis of glycosidic bonds. The impressive assortment of glycosidases in marine organisms clearly indicates that the potential biodiversity of these enzymes is still largely unexplored and that potential applications of biocatalysts from the sea will increase in the near future.
authors.affiliation: Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, I-80078 Pozzuoli (Napoli), Italy. antonio.trincone@icb.cnr.it
authors.forename: Antonio
authors.initials: A
authors.lastname: Trincone
authors.forename: Annabella
authors.initials: A
authors.lastname: Tramice
authors.forename: Assunta
authors.initials: A
authors.lastname: Giordano
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Andreotti
mesh_terms: D000818:Animals; D001048:Aplysia / Q000201:enzymology*; D001709:Biotechnology; D002240:Carbohydrate Sequence; D017753:Ecosystem; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000378:metabolism; D006031:Glycosylation; D008386:Marine Biology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis / Q000737:chemistry; D000520:alpha-Glucosidases / Q000032:analysis / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D006026:Glycoside Hydrolases; D000520:alpha-Glucosidases; D001616:beta-Galactosidase