title: Significant relation of tissue inhibitor of matrix metalloproteinase-2 and its combination with matrix metalloproteinase-2 to survival of patients with cancer of uterine cervix.
abstract: Tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) has high affinity for matrix metalloproteinase-2 (MMP-2). Few studies simultaneously investigate their implication in prognosis of patients with cervical cancer. We used reverse transcription-polymerase chain reaction and immunohistochemical method for cervical tissues and microarrays to investigate the association among TIMP-2, MMP-2, clinicopathological parameters, and prognosis of patients with cancer. Our results showed that cancer tissues exhibited less TIMP-2 expression and patients with pelvic lymph node metastasis had less TIMP-2 expression. Positive TIMP-2 constellated with negative MMP-2 indicated lower recurrence probability and better overall survival. The protective effect of TIMP-2 expression may overcome the adverse effect of MMP-2 expression in terms of disease-free interval and overall survival while neither TIMP-2 nor MMP-2 alone can be used to predict outcome. We suggest that following patients other than those with positive TIMP-2 and negative MMP-2 expression more closely and intensely may improve their prognosis.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan. lin12st653@yahoo.com.tw
authors.forename: Po-Hui
authors.initials: PH
authors.lastname: Wang
authors.forename: Jiunn-Liang
authors.initials: JL
authors.lastname: Ko
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Hsiu-Ting
authors.initials: HT
authors.lastname: Tsai
authors.forename: Yi-Torng
authors.initials: YT
authors.lastname: Tee
authors.forename: Chih-Ping
authors.initials: CP
authors.lastname: Han
authors.forename: Long-Yau
authors.initials: LY
authors.lastname: Lin
authors.forename: Shiuan-Chih
authors.initials: SC
authors.lastname: Chen
authors.forename: Yang-Tse
authors.initials: YT
authors.lastname: Shih
mesh_terms: D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D012334:RNA, Neoplasm; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2