title: [Prokaryotic expression and biological activity of recombinant human IL-17F/His protein].
abstract: OBJECTIVE
  To Prepare recombinant human IL-17F/His protein and investigate its biological activity in vitro.
  
  
  METHODS
  The gene region of human IL-17F was cloned by RT-PCR. After identification by sequencing, the hIL-17F gene encoding function domain was cloned into expression plasmid PQE3.0 and transfected into E.coli M15. By the induction of Isopropyl-β-D-Thiogalacto-Pyranoside(IPTG), recombinant IL-17F/His protein was effectively expressed in E.coli M15. The recombinant protein was identified by Western blot.
  
  
  RESULTS
  After renaturation and purification by HiTrap(TM); affinity column, the recombinant protein can up-regulate macrophages to secret TNF-α, IL-6 and other relative cytokines. It also promoted proliferation of HeLa cells in vitro.
  
  
  CONCLUSIONS
  hIL-17F/His recombinant protein is of high biological activity, which can be used to make further study of its special characteristic.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital, Soochow University, Suzhou 215007, China. liugaoqin2006@sina.com
authors.forename: Gao-qin
authors.initials: GQ
authors.lastname: Liu
authors.forename: Hong-ya
authors.initials: HY
authors.lastname: Wu
authors.forename: Long-biao
authors.initials: LB
authors.lastname: Li
authors.forename: Zhen-ni
authors.initials: ZN
authors.lastname: Ma
authors.forename: Pei-rong
authors.initials: PR
authors.lastname: Lu
authors.forename: Xue-guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D015153:Blotting, Western / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D016207:Cytokines / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020381:Interleukin-17 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D009000:Monocytes; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D015850:Interleukin-6; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha