title: Relative time course of degeneration of different cochlear structures in the CD/1 mouse model of accelerated aging.
abstract: Presbycusis (age-related hearing loss) can result from various cochlear pathologies. We have studied the time course of degeneration in a mouse that shows accelerated presbycusis, the CD/1 mouse, as a possible model to investigate stem-cell strategies to prevent or ameliorate presbycusic changes. CD/1 mice from 0 to 72 weeks old were examined by light and electron microscopy. Early pathological changes were detected in basal turn spiral ligament fibrocytes and spiral ganglion, but the latter was variable as both satellite cells and neurons were normal in some cochleae. Light microscopic counts in the spiral ligament of 20-week-old mice revealed that of the five main types (types I-V), only type V fibrocytes showed no reduction in numbers compared with 3-week-old animals, and type IV showed the greatest losses. However, all types of fibrocyte showed subtle damage when examined using electron microscopy, in the form of swollen mitochondria, as early as 2 weeks. The extent of mitochondrial damage showed a degree of correspondence with the light microscopic pattern of fibrocyte loss in that types III and IV fibrocytes had the most abnormal mitochondria and type V the least, especially at early stages. By 10-15 weeks, ultrastructural features of fibrocyte damage were similar to longer term changes reported in gerbils. Stria vascularis, spiral ganglion and hair cells showed few consistent early signs of damage but became increasingly affected, lagging behind the fibrocyte damage. Our data suggest that fibrocyte pathology may precede other presbycusic changes; breakdown of homeostatic mechanisms to which they contribute may cause the subsequent degeneration of the hair cells. Overall, there were many similarities to presbycusic changes in other rodents and humans. Therefore, the features of accelerated aging in this mouse make it a suitable model for rapidly assessing possible strategies to prevent or ameliorate presbycusic changes.
authors.affiliation: Institute for Science and Technology in Medicine and the School of Life Sciences, Keele University, Staffordshire, UK.
authors.forename: Shanthini
authors.initials: S
authors.lastname: Mahendrasingam
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Macdonald
authors.forename: David N
authors.initials: DN
authors.lastname: Furness
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D002452:Cell Count; D003051:Cochlea / Q000473:pathology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D006198:Hair Cells, Auditory / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008817:Mice, Mutant Strains; D008928:Mitochondria / Q000473:pathology / Q000648:ultrastructure; D011304:Presbycusis / Q000473:pathology*; D013136:Spiral Ganglion / Q000473:pathology / Q000648:ultrastructure; D013997:Time Factors