title: Multilayered microspheres for the controlled release of growth factors in tissue engineering.
abstract: Tissue regeneration may be stimulated by growth factors but to be effective, this delivery must be sustained and requires delivery vehicles that overcome the short half-life of these molecules in vivo. One promising approach is to couple growth factors to the biomaterial surface so that they are readily bioavailable. Here the layer-by-layer process was used to construct a multilayered polyelectrolyte delivery system on the surface of poly(lactic-co-glycolic) acid constructs. The system was first optimized on a planar surface before translation to a 3D microsphere system. The layers incorporated heparin to facilitate the loading of basic fibroblast growth factor and increase growth factor stability. Cross-linked capping layers also reduced any burst release. The model growth factor was released in a sustained manner and stimulated significantly higher cell proliferation in vitro on release compared with the addition of the growth factor heparin complex free in solution, demonstrating the promise of this approach.
authors.affiliation: Particulate Fluids Processing Centre, Department of Chemical and Biomolecular Engineering, University of Melbourne, Melbourne, Australia.
authors.forename: Dewi P
authors.initials: DP
authors.lastname: Go
authors.forename: Sally L
authors.initials: SL
authors.lastname: Gras
authors.forename: Debapriya
authors.initials: D
authors.lastname: Mitra
authors.forename: Thanh H
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Stevens
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Cooper-White
authors.forename: Andrea J
authors.initials: AJ
authors.lastname: O'Connor
mesh_terms: D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D019344:Lactic Acid / Q000737:chemistry; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid