title: Arabidopsis NADPH oxidases, AtrbohD and AtrbohF, are essential for jasmonic acid-induced expression of genes regulated by MYC2 transcription factor.
abstract: To clarify genetically the involvement of two Arabidopsis NADPH oxidases (AtrbohD and AtrbohF) in the jasmonic acid (JA) signaling pathway, we characterized single knockout mutants lacking either Atrboh. The accumulation of reactive oxygen species (ROS) and expression of the genes regulated by MYC2, a transcription factor involved in the JA-evoked response, were significantly suppressed by treatment with methyl JA (MeJA) in both mutants. Further experiments using knockout mutants lacking CORONATINE-INSENSITIVE1 (COI1), a master regulator of the JA-evoked response, and MYC2 indicated a possibility that the production of ROS via Atrbohs depends on the function of COI1, but not MYC2.
authors.affiliation: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara 631-8505, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Maruta
authors.forename: Takahiro
authors.initials: T
authors.lastname: Inoue
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tamoi
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Yabuta
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shigeoka
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism / Q000502:physiology*; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055786:Gene Knockout Techniques; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; C473611:MYC2 protein, Arabidopsis; D054883:Oxylipins; D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; C011006:jasmonic acid; C072239:methyl jasmonate; D019255:NADPH Oxidases