title: Defense response of a pepper cultivar cv. Sy-2 is induced at temperatures below 24°C.
abstract: Temperature is one of the most important environmental factors that influence plant growth and development. Recent studies imply that plants show various responses to non-extreme ambient temperatures. Previously, we have found that a pepper cultivar cv. Sy-2 (Capsicum chinense) shows developmental defects at temperatures below 24°C. In this study, to gain new insights into the temperature sensitivity of cv. Sy-2, temperature-sensitive genes were screened using microarray techniques. At restrictive temperature of 20°C, almost one-fourth of the 411 up-regulated genes were defense related or predicted to be defense related. Further expression analyses of several defense-related genes showed that defense-related genes in cv. Sy-2 were constitutively expressed at temperatures below 24°C. Moreover, accumulation of high level of salicylic acid (SA) in cv. Sy-2 grown at 20°C suggests that the defense response is activated in the absence of pathogens. To confirm that the defense response is induced in cv. Sy-2 below 24°C, we evaluated the resistance to biotrophic bacterial pathogen Xanthomonas campestris pv. vesicatoria and necrotrophic fungal pathogen Cercospora capsici. Cv. Sy-2 showed enhanced resistance to X. campestris pv. vesicatoria, but not to C. capsici.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan. koeda.sota@t02.mbox.media.kyoto-u.ac.jp
authors.forename: Sota
authors.initials: S
authors.lastname: Koeda
authors.forename: Munetaka
authors.initials: M
authors.lastname: Hosokawa
authors.forename: Byoung-Cheorl
authors.initials: BC
authors.lastname: Kang
authors.forename: Chihiro
authors.initials: C
authors.lastname: Tanaka
authors.forename: Doil
authors.initials: D
authors.lastname: Choi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sano
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiina
authors.forename: Motoaki
authors.initials: M
authors.lastname: Doi
authors.forename: Susumu
authors.initials: S
authors.lastname: Yazawa
mesh_terms: D002212:Capsicum / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D013696:Temperature; D015854:Up-Regulation / Q000235:genetics
chemical_list: D020156:Salicylic Acid