title: T-2 toxin induces degenerative articular changes in rodents: link to Kaschin-Beck disease.
abstract: Osteoarthritis (OA) is a degenerative joint disease that is characterized by joint pain and a progressive loss of articular cartilage. Kaschin-Beck Disease is a form of endemic OA in China whose etiology is unclear, but epidemiological data indicate a possible link to trichothecenes mycotoxin exposure. In vitro, T-2 toxin, a trichothecenes mycotoxin, has been demonstrated to inhibit aggrecan synthesis and promote aggrecanase and pro-inflammatory cytokine production in cultured chondrocytes. To assess the effects of T-2 toxin on articular cartilage in vivo, Wistar rats were fed a diet containing T-2 toxin (100 ng/kg chow) for six and ten months. Following six months of T-2 toxin exposure, histopathological changes in femorotibial cartilage were characterized by chondrocyte degeneration/necrosis and loss, chondrocyte clones, and loss of proteoglycan staining of articular cartilage, sometimes involving the entire thickness of the cartilage in the tibial plateaus and femoral condyles. By ten months, in addition to these changes, there was evidence of cartilage fibration in some rats. In conclusion, T-2 toxin exposure in rats induced degenerative lesions in articular cartilage similar to spontaneous OA, lending support to an etiologic role of mycotoxins in Kaschin-Beck Disease. T-2 toxin-induced degenerative joint disease may be a useful model of metabolic polyarticular OA.
authors.affiliation: The Center for Endemic Disease Control, National Center for Disease Prevention and Control, Harbin Medical University, Heilongjiang, China. lhwanganna@126.com
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Fu
authors.forename: Yu Xia
authors.initials: YX
authors.lastname: Shi
authors.forename: Wen Ge
authors.initials: WG
authors.lastname: Wang
mesh_terms: D053674:Aggrecans / Q000096:biosynthesis; D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000473:pathology; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D057767:Kashin-Beck Disease / Q000473:pathology; D008297:Male; D010003:Osteoarthritis / Q000139:chemically induced / Q000473:pathology*; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013605:T-2 Toxin / Q000633:toxicity*; D013977:Tibia / Q000187:drug effects / Q000473:pathology
chemical_list: D053674:Aggrecans; D011509:Proteoglycans; D013605:T-2 Toxin