title: Expression patterns of imprinted gene Inpp5f-v3 during mouse brain development.
abstract: Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis revealed that only one transcript approx 2.7 kb of Inpp5f-v3 was detected in brain. The signals were only observed in brain by the whole-mount in situ hybridization at embryonic day 11.5 (E11.5). The results of quantitative real-time PCR (QRT-PCR) showed that the expression of Inpp5f-v3 increased gradually from the E11.5 to E17.5 and reached the highest at E17.5, then decreased at E18.5 during the brain development. Inpp5f-v3 gene was strongly expressed in the cerebral cortex, olfactory bulb, external germinal layer of cerebellum and ventricular zone (Vz) during the embryonic development (E15.5-E19.5), whereas the expression increased in the olfactory bulb and the cerebellum after birth by using in situ hybridization. The results also demonstrated that the expression of Inpp5f-v3 gene mainly located in olfactory bulb and hippocampus at postnatal day 7 (P7) and adulthood. These results suggest that Inpp5f-v3 is specifically expressed in mouse brain, and may function in the development of mouse brain.
authors.affiliation: Department of Life Science and Engineering, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, 150001, Heilongjian, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.forename: He
authors.initials: H
authors.lastname: Hongjuan
authors.forename: Xing
authors.initials: X
authors.lastname: Yanjiang
authors.forename: Han
authors.initials: H
authors.lastname: Zhengbin
authors.forename: Li
authors.initials: L
authors.lastname: Kai
authors.forename: Zhang
authors.initials: Z
authors.lastname: Fengwei
authors.forename: Hou
authors.initials: H
authors.lastname: Jing
authors.forename: Wu
authors.initials: W
authors.lastname: Qiong
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000196:embryology* / Q000378:metabolism; D002540:Cerebral Cortex / Q000196:embryology* / Q000378:metabolism; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D000072181:Inositol Polyphosphate 5-Phosphatases; D051379:Mice; D008810:Mice, Inbred C57BL; D009830:Olfactory Bulb / Q000196:embryology* / Q000378:metabolism; D009928:Organ Specificity; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases