title: Imatinib induces autophagy through BECLIN-1 and ATG5 genes in chronic myeloid leukemia cells.
abstract: Imatinib is a chemotherapeutic drug used for the treatment of chronic myeloid leukemia (CML). Recent data showed imatinib-induced cell death in various types of cancers. Autophagy is the physiological process in which cellular components are broken down by the lysosomal activation. In this study, we aimed to examine the effects of imatinib on autophagy in addition to apoptosis in CML cells. Results suggested that imatinib induces autophagy in CML cells through inducing over-expression of BECLIN-1 and ATG5 genes with the statistical significance. Our results demonstrated that autophagy might be involved in imatinib-induced cell death.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Izmir Institute of Technology, Izmir, Turkey.
authors.forename: Geylani
authors.initials: G
authors.lastname: Can
authors.forename: Huseyin Atakan
authors.initials: HA
authors.lastname: Ekiz
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Baran
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects* / Q000235:genetics; D000071187:Autophagy-Related Protein 5; D000071186:Beclin-1; D001549:Benzamides; D045744:Cell Line, Tumor; D006801:Humans; D000068877:Imatinib Mesylate; D015151:Immunoblotting; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000378:metabolism*; D008565:Membrane Proteins / Q000187:drug effects*; D008869:Microtubule-Associated Proteins / Q000187:drug effects*; D010879:Piperazines / Q000494:pharmacology* / Q000627:therapeutic use; D011743:Pyrimidines / Q000494:pharmacology* / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C502778:ATG5 protein, human; D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D000071187:Autophagy-Related Protein 5; C491997:BECN1 protein, human; D000071186:Beclin-1; D001549:Benzamides; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate