title: Influence of sCD40L on gastric cancer cell lines.
abstract: The CD40 signaling pathway plays a key role in tumor cell proliferation, differentiation, and apoptosis. Gastric cancer usually possesses a higher level of CD40 expression than normal tissue. We evaluated inhibition of soluble CD40 ligand (sCD40L) in apoptosis induction of gastric cancer cells. Gastric cancer cells (AGS and BGC-823) were incubated with sCD40L. Cell viability and cell cycle were determined by methyl-tetrazolium (MTT) assay and flow cytometry, respectively. The results showed that sCD40L hindered cell growth, arrested cells at G0/G1 phase and induced apoptosis. In conclusion, sCD40L suppress growth of gastric cancer cells through apoptosis induction and cell cycle quiescence. This work will provided a new approach to gene therapy of gastric cancer.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Suzhou 215006, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Wei-Chang
authors.initials: WC
authors.lastname: Chen
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Pang
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Tian
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001681:Biological Assay; D023201:CD40 Ligand / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D012995:Solubility / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D023201:CD40 Ligand