title: The association between cyclooxygenase-2 (COX-2/PTGS2) gene polymorphism and osteoarthritis.
abstract: OBJECTIVE
  This study aims to investigate the relationship between the risk for the development of osteoarthritis and Cyclooxygenase-2 (COX 2) -765G>C gene polymorphism.
  
  
  METHODS
  We included a total of 100 osteoarthritis patients (18 males, 82 females; mean age 60.4±8.4 years; range 41 to 81 years) who were treated in the Physical Therapy Clinic and 100 healthy subjects without a history of arthritis (40 males, 60 females; mean age 30.9±7.5 years; range 16 to 48 years) in our study between September 2006 and May 2008. The frequency of -765G>C gene polymorphism in the COX-2 promoter region was investigated in the osteoarthritis patients and the control group without a history of arthritis using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The data were analysed with chi-square and logistic regression analysis.
  
  
  RESULTS
  The frequencies of -765G>C polymorphism for GG, GC and CC genotypes were found to be 54%, 35%, and 11% in the control group and 48%, 34% and 18% in the osteoarthritis group, respectively.
  
  
  CONCLUSIONS
  Based on the data obtained, it can be stated that there is no significant relation between COX-2 -765G>C polymorphism and osteoarthritis disease. Furthermore, this study presents the first results of COX-2 promoter variant in Turkish patients with osteoarthritis.
authors.affiliation: Department of Biochemistry, Mersin University Pharmacy Faculty, Mersin, Turkey.
authors.forename: Volkan G
authors.initials: VG
authors.lastname: Güler
authors.forename: Serap
authors.initials: S
authors.lastname: Yalın
authors.forename: Mehmet
authors.initials: M
authors.lastname: Berköz
authors.forename: Ozlem Bölgen
authors.initials: OB
authors.lastname: Cimen
authors.forename: Erdinç
authors.initials: E
authors.lastname: Yalın
authors.forename: Pelin
authors.initials: P
authors.lastname: Eroğlu
authors.forename: Arzu
authors.initials: A
authors.lastname: Kanık
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D055815:Young Adult
chemical_list: D051546:Cyclooxygenase 2