title: [Effects of salidroside on proliferation, apoptosis, phagocytosis, ROS and NO production of murine peritoneal macrophages in vitro].
abstract: OBJECTIVE
  To investigate the effects of salidroside(Sal) on proliferation, apoptosis, phagocytosis, the production of ROS and NO of murine peritoneal macrophages in vitro as well as its immunoregulation.
  
  
  METHODS
  The single cell suspension of murine peritoneal macrophages was prepared under sterile condition, then co-cultured with different concentrations of Sal(80, 160 and 320 μmol/L)for 4 hours prior to stimulation with LPS and IFN-γ, the proliferation of macrophages was measured by MTT colorimetry. The effect of Sal on the apoptosis of Sytox® Green-labelled peritoneal macrophages induced by CHX was detected by Fluorescence enzyme-labelled meter. FCM was used to detect the effect of Sal on phagocytosis of peritoneal macrophages. Fluorescence enzyme-labelled meter was used to measure the effects of Sal on ROS of H(2);DCFDA-labelled macrophages induced by LPS and IFN-γ. Griess Gragent was used to detect the role of Sal in production of NO in peritoneal macrophages activated by LPS and IFN-γ.
  
  
  RESULTS
  MTT result demonstrated that Sal could promote the proliferation of peritoneal macrophages activated by LPS and IFN-γ at the final concentrations of 80, 160, 320 μmol/L, respectively (P<0.05). The result of Fluorescence enzyme-labelled meter detected showed that Sal at the final concentration of 160 μmol/L could inhibit apoptosis of peritoneal macrophages induced by CHX(P<0.01). FCM analysis showed that different concentrations of Sal significantly promoted the phagocytosis of peritoneal macrophages which include un-activated and activated by LPS and IFN-γ(P<0.05). Fluorescence enzyme-labelled meter showed that Sal could reduce the production of ROS in activated peritoneal macrophages induced by LPS and IFN-γ(P<0.05). Sal also increased the production of NO in activated peritoneal macrophages induced by LPS and IFN-γ(P<0.05).
  
  
  CONCLUSIONS
  Sal can promote proliferation of peritoneal macrophages stimulated by LPS and IFN-γ, and it can inhibit apoptosis of peritoneal macrophages induced by CHX, Sal also can promote the phagocytosis of peritoneal macrophages which include un-activated and activated by LPS and IFN-γ, Sal can reduce the production of ROS in activated peritoneal macrophages induced by LPS and IFN-γ, while Sal can promote the production of NO in activated peritoneal macrophages induced by LPS and IFN-γ.
authors.affiliation: Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. dhyss8625@126.com
authors.forename: Sha-sha
authors.initials: SS
authors.lastname: Ye
authors.forename: Yao-ying
authors.initials: YY
authors.lastname: Zeng
authors.forename: Le-le
authors.initials: LL
authors.lastname: Yin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D007371:Interferon-gamma / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D010636:Phenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D005960:Glucosides; D008070:Lipopolysaccharides; D010636:Phenols; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D007371:Interferon-gamma; C009172:rhodioloside