title: The potency of hyaluronan of different molecular weights in the stimulation of blood phagocytes.
abstract: The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970  kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52  kDa having the highest potency and high MW HA 970  kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA.
authors.affiliation: Institute of Biophysics, The Academy of Sciences of the Czech Republic, v. v. i., Královopolská 135, 612 65 Brno, Czech Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Safrankova
authors.forename: Silvie
authors.initials: S
authors.lastname: Gajdova
authors.forename: Lukas
authors.initials: L
authors.lastname: Kubala
mesh_terms: D015703:Antigens, CD / Q000097:blood; D039481:CD11b Antigen / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood; D015550:Cell Degranulation / Q000187:drug effects; D058851:GPI-Linked Proteins / Q000097:blood; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D007249:Inflammation / Q000097:blood / Q000209:etiology; D008970:Molecular Weight; D010586:Phagocytes / Q000187:drug effects* / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000097:blood; D017463:Receptors, Complement 3b / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; C504120:CEACAM8 protein, human; C509991:CR1 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; C509295:ITGAM protein, human; D017382:Reactive Oxygen Species; D017463:Receptors, Complement 3b; D014409:Tumor Necrosis Factor-alpha; D006820:Hyaluronic Acid