title: Production of mouse embryonic stem cell lines from maturing oocytes by direct conversion of meiosis into mitosis.
abstract: ESCs are most commonly derived from embryos originating from oocytes that reached metaphase II. We describe here a novel approach where ESCs with all pluripotency parameters were established from oocytes in which metaphase I was converted, from the cell cycle perspective, directly into metaphase II-like stage without the intervening anaphase to telophase I transition. The resulting embryos initiate development and reach the blastocyst stage from which the ESC lines are then established. Thus, our approach could represent an ethically acceptable method that can exploit oocytes that are typically discarded in in vitro fertilization clinics. Moreover, our results also indicate that the meiotic cell cycle can be converted into mitosis by modulating chromosomal contacts that are typical for meiosis with subsequent licensing of chromatin for DNA replication.
authors.affiliation: Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Fulka
authors.forename: Michiko
authors.initials: M
authors.lastname: Hirose
authors.forename: Kimiko
authors.initials: K
authors.lastname: Inoue
authors.forename: Narumi
authors.initials: N
authors.lastname: Ogonuki
authors.forename: Noriko
authors.initials: N
authors.lastname: Wakisaka
authors.forename: Shogo
authors.initials: S
authors.lastname: Matoba
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ogura
authors.forename: Tibor
authors.initials: T
authors.lastname: Mosko
authors.forename: Tomas
authors.initials: T
authors.lastname: Kott
authors.forename: Josef
authors.initials: J
authors.lastname: Fulka
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D005260:Female; D008297:Male; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D009866:Oogenesis / Q000502:physiology; D010312:Parthenogenesis / Q000502:physiology; D011247:Pregnancy