title: Epiallele quantification using molecular inversion probes.
abstract: The location and level of DNA methylation within a genome is emerging as an important biomarker for cancer diagnosis. Despite its potential, it is difficult to comprehensively analyze the epialleles that are often found in a biological sample. Therefore, an assay utilizing molecular inversion probes was designed and used to expose and quantify epialleles in heterogeneously methylated bisulphite treated genomic DNA. Different CpG dinucleotides were able to be rapidly quantified with high resolution, sensitivity and specificity over a large dynamic range using rapid flow cytometric readout of multiplexable microbead DNA biosensors.
authors.affiliation: Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane Qld, 4072 Australia.
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Palanisamy
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Connolly
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D000483:Alleles; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D005434:Flow Cytometry; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008863:Microspheres; D015345:Oligonucleotide Probes / Q000235:genetics*; D013997:Time Factors
chemical_list: D005819:Genetic Markers; D015345:Oligonucleotide Probes