title: MicroRNA-10b induces glioma cell invasion by modulating MMP-14 and uPAR expression via HOXD10.
abstract: MicroRNAs are small endogenous noncoding RNAs, which modulate target gene expression by binding with target mRNA sequences in the 3'untranslated region (UTR) with an imperfect complementarity that inhibits the mRNA translation. Many microRNAs have been reported to function as tumor oncogenes or anti-oncogenes. Recently, more and more microRNAs have been reported to contribute to a tumor's invasive potential. Here, we show that microRNA-10b (miR-10b) was over-expressed in glioma samples and directly associated with the glioma's pathological grade and malignancy. We also found that miR-10b induced glioma cell invasion by modulating tumor invasion factors MMP-14 and uPAR expression via the direct target HOXD10. The miR-10b/HOXD10/MMP-14/uPAR signaling pathway might contribute to the invasion of glioma. Accordingly, glioma cells lost their invasive ability when treated with specific antisense oligonucleotides (miR-10b inhibitors), suggesting that miR-10b could be used as a new bio-target to cure glioma.
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Guan
authors.initials: G
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.forename: Junxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Yongping
authors.initials: Y
authors.lastname: You
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Yang
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D055293:Receptors, Urokinase Plasminogen Activator / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D018398:Homeodomain Proteins; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D055293:Receptors, Urokinase Plasminogen Activator; D014157:Transcription Factors; C072064:HOXD10 protein, human; D053511:Matrix Metalloproteinase 14