title: tert-butyl hydroperoxide, an organic peroxide, causes temporary delay in hair growth in a neonatal rat model.
abstract: tert-butyl hydroperoxide (tBHP), an organic peroxide, has been shown to cause irreversible damage to keratinocytes in vitro with prolonged administration at high concentrations, and reversible damage with short-term administration at low concentrations. To investigate the effects of tBHP on keratinocytes in vivo, we analysed hair growth in tBHP-treated neonatal rats. Sprague-Dawley and Long-Evans rat pups were injected subcutaneously with tBHP or vehicle once daily for 6 days, and hair growth was monitored. The tBHP-treated rats had a significant delay in hair growth. However, this delay reversed within days, and the hair coats, including hair pigmentation, of tBHP-treated and sham-treated rats were indistinguishable 2 weeks later. Histological analysis and BrdU labelling of S phase cells confirmed the delay in hair-follicle growth and its reversal in tBHP-treated rats. Our results indicated that the changes incurred in hair follicles by short-term use of high-dose oxidants in vivo are temporary and reversible.
authors.affiliation: Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Wikramanayake
authors.forename: J
authors.initials: J
authors.lastname: Simon
authors.forename: L M
authors.initials: LM
authors.lastname: Mauro
authors.forename: C I
authors.initials: CI
authors.lastname: Perez
authors.forename: B
authors.initials: B
authors.lastname: Roberts
authors.forename: G
authors.initials: G
authors.lastname: Elgart
authors.forename: E
authors.initials: E
authors.lastname: Alvarez-Connelly
authors.forename: L A
authors.initials: LA
authors.lastname: Schachner
authors.forename: J J
authors.initials: JJ
authors.lastname: Jimenez
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D018859:Hair Follicle / Q000187:drug effects; D015603:Keratinocytes / Q000187:drug effects*; D023421:Models, Animal; D010858:Pigmentation / Q000187:drug effects; D051381:Rats; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
chemical_list: D020122:tert-Butylhydroperoxide