title: [The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval].
abstract: OBJECTIVE
  To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the mouse liver and postmortem interval (PMI).
  
  
  METHODS
  Sixty NIH mice were sacrificed by cervical dislocation and suffocation, and then placed into 10 degrees C and 25 degrees C temperature-controlling systems. The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction (RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.
  
  
  RESULTS
  In the mouse liver, the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 degrees C temperature-controlling system and within 36 h postmortem in 25 degrees C temperature-controlling system. The amplification products showed a decreasing tendency.
  
  
  CONCLUSIONS
  Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI. GAPDH mRNA could be a new marker for estimation of PMI.
authors.affiliation: Department of Forensic Medicine, School of Basic Medical Science, Xinxiang Medical University, Xinxi-ang 453003, China. whyfine@163.com
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Wu
authors.forename: Ke-jie
authors.initials: KJ
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Guo
authors.forename: Juan-ning
authors.initials: JN
authors.lastname: Guo
authors.forename: Ai-ying
authors.initials: AY
authors.lastname: Fan
mesh_terms: D000818:Animals; D005260:Female; D049429:Forensic Pathology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011180:Postmortem Changes; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases