title: apoE expression in glomerulus and correlation with glomerulosclerosis induced by adriamycin in rats.
abstract: Lipid deposition in glomerulus plays an important role in the progression of glomerulosclerosis (GS), and apolipoprotein E (apoE) is an important protein in cholesterol homeostasis. This investigation was performed to explore whether there exists an association between apoE and GS susceptibility. Eighty Wistar rats were randomly divided into two groups: sham operation group and glomerulosclerosis model group, n = 40. The GS disease in rat was induced by uninephrectomy and injecting adriamycin (5 mg/kg) through the tail vein. At the end of 9 and 13 weeks, 20 rats in each group were killed and the relative samples were collected. Serum creatinine, blood urea nitrogen, and 24-h urine protein were determined. Immunohistochemical analysis was performed on renal tissue to detect the expression of apoE, collagen IV, fibronectin, α-smooth muscle actin, and transforming growth factor-β1 in glomerulus. Real-time reverse transcription polymerase chain reaction was conducted to detect the apoE mRNA expression in renal tissue. Compared with sham operation group at the end of 9/13 weeks, glomerulosclerosis model group exhibited levels of serum creatinine, blood urea nitrogen, 24-h urine protein, and a glomerulosclerosis index that were significantly elevated ( p < 0.01), and collagen IV, fibronectin, α-smooth muscle actin, and transforming growth factor-β1 protein expression and apoE expression (protein and mRNA) were significantly upregulated (p < 0.01). In conclusion, apoE can increase the accumulation of extracellular matrix in glomerulus and may take part in the progression of GS.
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, PR China.
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Qin
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Lei
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Su
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Huang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001057:Apolipoproteins E / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism; D004317:Doxorubicin; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005923:Glomerulosclerosis, Focal Segmental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007677:Kidney Function Tests; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000199:Actins; D001057:Apolipoproteins E; D024141:Collagen Type IV; D004317:Doxorubicin; D005353:Fibronectins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C498862:Acta2 protein, rat