title: MicroRNA miR-125b is a prognostic marker in human colorectal cancer.
abstract: MicroRNAs (miRNAs) are small, non-coding RNAs that can function as oncogenes or tumor suppressors in human cancer. Recent reports have highlighted the oncogenic aspects of microRNA miR-125b. However, the clinical significance of miR-125b in gastrointestinal cancers has not been sufficiently investigated. To this end, we analyzed miR-125b expression in colorectal cancer cases. Quantitative RT-PCR was used to evaluate miR-125b expression in 89 colorectal cancer cases to determine the clinicopathological significance of miR-125b expression. The high miR-125b expression group showed a greater incidence of advanced tumor size and tumor invasion compared to the low miR-125b expression group (P<0.05). In addition, the high miR-125b expression group had a significantly poorer prognosis compared to the low expression group (P<0.05). Multivariate analysis indicated that high miR-125b expression was an independent prognostic factor for survival. Our analysis of miR-125b focused on the miR-125b/p53 pathway. In vitro assays revealed that overexpression of miR-125b repressed the endogenous level of p53 protein in human colorectal cancer cells. These data show that miR-125b is directly involved in cancer progression and is associated with poor prognosis in human colorectal cancer. Our findings suggest that miR-125b could be an important prognostic indicator for colorectal cancer patients.
authors.affiliation: Department of Surgery and Molecular Oncology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumibaru, Beppu, Oita 874-0838, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Nishida
authors.forename: Takehiko
authors.initials: T
authors.lastname: Yokobori
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
authors.forename: Tomoya
authors.initials: T
authors.lastname: Sudo
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Tanaka
authors.forename: Kohei
authors.initials: K
authors.lastname: Shibata
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D018450:Disease Progression; D005260:Female; D016158:Genes, p53; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger