title: A Microsphere-Based Suspension Array for Blood Group Molecular Typing: An Update.
abstract: SUMMARY: BACKGROUND: In a previous publication we described a method for Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, K/k, Kp(a)/Kp(b), Js(a)/Js(b), Co(a)/Co(b), and Lu(a)/Lu(b) genotyping based on a microsphere suspension array. Here, an improved version of the assay is presented. METHODS: TWO MULTIPLEX POLYMERASE CHAIN REACTIONS (PCR) WERE DEVELOPED: one for amplification of samples routinely tested and the other for those systems that are tested less frequently. Each biotinylated PCR product is hybridized in a single multiplex assay. A total of 2,020 samples were analyzed, and the genotypes were compared to the blood group phenotypes. RESULTS: There have been no discrepancies with the serology results other than null and/or weak phenotypes. CONCLUSION: In its present form, the method presented here has the capacity to genotype hundreds of a samples in few hours with a high concordance rate with serology.
authors.affiliation: Organ and Tissue Transplantation Immunology - Regenerative Medicine Department, Milan, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Drago
authors.forename: Katerina
authors.initials: K
authors.lastname: Karpasitou
authors.forename: Laura
authors.initials: L
authors.lastname: Spinardi
authors.forename: Loretta
authors.initials: L
authors.lastname: Crespiatico
authors.forename: Mario
authors.initials: M
authors.lastname: Scalamogna
authors.forename: Francesca
authors.initials: F
authors.lastname: Poli