title: Utilization of IκB-EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity.
abstract: NF-κB regulates several important expressions, such as cytokine release, anti-apoptosis, adhesion molecule expression, and cell cycle processing. Several NF-κB inhibitors have been discovered as an anti-tumor or anti-inflammatory drug. The activity of NF-κB transcription factor is negatively regulated by IκB binding. In this study, IκB assay system was established and IκB-EGFP fusion protein was used as an indicator to monitor the effects of substances on the IκB degradation. The results indicated that the chosen hydroquinone could inhibit the IκB degradation and cause the cell de-attachment from the bottom of culture plate. In addition, this system could also monitor the IκB degradation of microbial metabolite of natural mixtures of propolis. Thus, the IκB assay system may be a good system for drug discovery related to microbial metabolite.
authors.affiliation: Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Lin
authors.affiliation: Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yen-Shun
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Electronics Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Jui-Hung
authors.initials: JH
authors.lastname: Hsieh
authors.affiliation: Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Ching-Min
authors.initials: CM
authors.lastname: Lin
authors.affiliation: Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Wu
authors.affiliation: Animal Cancer Center, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Chen-Si
authors.initials: CS
authors.lastname: Lin
authors.affiliation: Animal Cancer Center, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Rea-Min
authors.initials: RM
authors.lastname: Chu
authors.affiliation: Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.|Department of Biological Science and Technology, National Chiao Tung University, 300 Room 205 Zhu-Ming Building, 75 Bo-Ai Street, Hsinchu, Taiwan.
authors.forename: Kuang-Wen
authors.initials: KW
authors.lastname: Liao
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yuan-Hsun
authors.initials: YH
authors.lastname: Hsu