title: The Er71 is an important regulator of hematopoietic stem cells in adult mice.
abstract: The Ets transcription factor Er71 is an important regulator of endothelial and hematopoietic development during mammalian embryogenesis. However, the role of Er71 in adult hematopoiesis has remained unknown. We now first show that conditional deletion of Er71 in the hematopoietic system of adult mice results in a marked reduction (55%) in the number of hematopoietic stem cells (HSCs) that is likely due to increased cell death. Bone marrow transplantation (BMT) experiments further confirmed that Er71 is required for repopulation of HSCs. In addition, Er71(+/-) mice exhibited a slight decrease (37%) in the number of HSCs than those of Er71(+/+) mice, indicating that the function of Er71 in HSC maintenance is dependent on gene dosage. Moreover, Er71 was shown to be required for Tie2 expression, which contributes to HSC maintenance. Our results thus suggest the role of a single transcription factor in controlling HSCs through regulation of Tie2 expression in adult animals.
authors.affiliation: National Creative Research Initiatives Center, Department of Biological Sciences, Graduate School of Nanoscience and Technology (WCU), Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Korea.
authors.forename: Dongjun
authors.initials: D
authors.lastname: Lee
authors.forename: Tackhoon
authors.initials: T
authors.lastname: Kim
authors.forename: Dae-Sik
authors.initials: DS
authors.lastname: Lim
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D046228:Microarray Analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: C503994:ER71 protein, mouse; D014157:Transcription Factors