title: Effects of Antrodia camphorata on alcohol clearance and antifibrosis in livers of rats continuously fed alcohol.
abstract: Alcoholic fatty liver disease (AFLD) is the result of an excessive or chronic consumption of alcohol. Nine male Wistar rats per group were randomly assigned to one of the following drinking treatments: a 20% (w/w) alcohol solution (ALC); a 20% (w/w) alcohol solution cotreated with 0.25 g silymarin/kg BW/day; or a 20% (w/w) alcohol solution cotreated with 0.025 g Niuchangchih ( Antrodia camphorata )/kg BW/day for 4 weeks. Rats with cotreatments of silymarin or Niuchangchih had smaller (p < 0.05) relative liver size, less (p < 0.05) liver lipid accumulation, and lower (p < 0.05) liver damage indices [aspartate aminotransferase (AST) and alkaline phosphatase (ALP) values]. In the regulation of alcohol metabolism, the lower serum alcohol level was observed only in alcohol-fed rats supplemented with Niuchangchih. Meanwhile, cotreatment of silymarin or Niuchangchih increased (p < 0.05) CAT and ALDH activities but did not (p > 0.05) affect ADH and CYP2E1 expressions, which accelerate alcohol metabolism in the body. Additionally, neither silymarin nor Niuchangchih (p > 0.05) influenced serum/hepatic MMP-2 activities and NF-κB, AP1, and α-SMA gene expressions, but serum/hepatic MMP-9 activities and TNF-α, KLF-6, and TGF-β1 gene expressions of alcohol-fed rats were down-regulated (p < 0.05) by silymarin or Niuchangchih, which also could explain the lower liver damage observed in rats chronically fed alcohol.
authors.affiliation: Biotechnology Division, Taiwan Agricultural Research Institute , Council of Agriculture, Taichung County, Taiwan.
authors.forename: Min-Tze
authors.initials: MT
authors.lastname: Wu
authors.forename: Bor-Show
authors.initials: BS
authors.lastname: Tzang
authors.forename: Yuan-Yen
authors.initials: YY
authors.lastname: Chang
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Chiu
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Kang
authors.forename: Chia-Hsin
authors.initials: CH
authors.lastname: Huang
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D055491:Antrodia / Q000737:chemistry*; D001483:Base Sequence; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism; D017931:DNA Primers; D000431:Ethanol / Q000008:administration & dosage* / Q000493:pharmacokinetics; D008103:Liver Cirrhosis / Q000097:blood / Q000201:enzymology / Q000517:prevention & control*; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012838:Silymarin / Q000008:administration & dosage
chemical_list: D017931:DNA Primers; D010936:Plant Extracts; D012838:Silymarin; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D020782:Matrix Metalloproteinases