title: [Histochemical and immunohistochemical analysis of ruptured atherosclerotic abdominal aortic aneurysm wall].
abstract: OBJECTIVE
  The main complication of the atherosclerotic abdominal aortic aneurysm (AAA) is her rupture that begins with lesion in intima and rupture. The purpose of this work was to determine immunocytochemical and morphofunctional characteristics of the cells in aortic wall in ruptured atherosclerotic abdominal aortic aneurysm.
  
  
  METHODS
  During the course of this study, 20 samples of atherosclerotic AAA were analyzed, all of them obtained during authopsy. The samples were fixed in 4% formalin and embedded in paraffin. Sections of 5 microm thickness were stained histochemically (of Heidenhain azan stain and Periodic acid Schiff--PAS stain) and immunocytochemically using a DAKO LSAB+/HRP technique to identify alpha-smooth muscle actin (alpha-SMA), vimentin, myosin heavy chains (MHC), desmin, S-100 protein, CD45 and CD68 (DAKO specification).
  
  
  RESULTS
  The results of our study showed that ruptured atherosclerotic AAA is characterized by a complete absence of endothelial cells, the disruption of basal membrane and internal elastic lamina, as well as a presence of the remains of hypocellular complicated atherosclerotic lesion in intima. On the plaque margins, as well as in the media, smooth muscle cells (SMCs) are present, which express a alpha-SMA and vimentin (but without MHC or desmin expression), as well as leukocyte infiltration, and a large number of foam cells. Some of the foam cells show a CD68- immunoreactivity, while the others show vimentin- and S-100 protein-immunoreactivity. Media is thinned out with a disorganized elastic lamellas, while adventitia is characterized by inflammatory inflitrate (infection).
  
  
  CONCLUSIONS
  Rupture of aneurysm occurs from the primary intimal disruption, which spreads into thinned out media and adventitia. Rupture is caused by unstable atherom, hypocellularity, loss of contractile characteristics of smooth muscle cells in intima and media, neovascularization of the media, as well as by the activity of the macrophages in the lesion.
authors.affiliation: Medicinski fakultetu Kragujevac, Katedra za histologiju i embriologiju, Kragujevac, Srbija. irena.vuk@gmail.com
authors.forename: Irena
authors.initials: I
authors.lastname: Tanasković
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mladenović-Mihailović
authors.forename: Slavica
authors.initials: S
authors.lastname: Usaj-Knezević
authors.forename: Vesna
authors.initials: V
authors.lastname: Stanković
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Aleksić
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kastratović
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Aleksić
authors.forename: Zorica
authors.initials: Z
authors.lastname: Lazić
authors.forename: Zorica
authors.initials: Z
authors.lastname: Mladenović-Bogdanović
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Zivanović
authors.forename: Janko
authors.initials: J
authors.lastname: Djurić
authors.forename: Ugljesa
authors.initials: U
authors.lastname: Jovicić
authors.forename: Marija
authors.initials: M
authors.lastname: Sorak
mesh_terms: D000199:Actins / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism* / Q000473:pathology; D001019:Aortic Rupture / Q000378:metabolism* / Q000473:pathology; D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male; D009418:S100 Proteins / Q000032:analysis; D000097382:CD68 Molecule
chemical_list: D000199:Actins; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D007381:Intermediate Filament Proteins; D017493:Leukocyte Common Antigens; D009418:S100 Proteins; C067980:CD68 antigen, human; D000097382:CD68 Molecule