title: Helicobacter pylori induction of eosinophil migration is mediated by the cag pathogenicity island via microbial-epithelial interactions.
abstract: The host immune response directed against Helicobacter pylori is ineffective in eliminating the organism and strains harboring the cag pathogenicity island augment disease risk. Because eosinophils are a prominent component of H. pylori-induced gastritis, we investigated microbial and host mechanisms through which H. pylori regulates eosinophil migration. Our results indicate that H. pylori increases production of the chemokines CCL2, CCL5, and granulocyte-macrophage colony-stimulating factor by gastric epithelial cells and that these molecules induce eosinophil migration. These events are mediated by the cag pathogenicity island and by mitogen-activated protein kinases, suggesting that eosinophil migration orchestrated by H. pylori is regulated by a virulence-related locus.
authors.affiliation: Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-2279, USA.
authors.forename: Toni A
authors.initials: TA
authors.lastname: Nagy
authors.forename: Shannon S
authors.initials: SS
authors.lastname: Allen
authors.forename: Lydia E
authors.initials: LE
authors.lastname: Wroblewski
authors.forename: David K
authors.initials: DK
authors.lastname: Flaherty
authors.forename: James C
authors.initials: JC
authors.lastname: Slaughter
authors.forename: Guillermo
authors.initials: G
authors.lastname: Perez-Perez
authors.forename: Dawn A
authors.initials: DA
authors.lastname: Israel
authors.forename: Richard M
authors.initials: RM
authors.lastname: Peek
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004804:Eosinophils / Q000382:microbiology*; D004847:Epithelial Cells / Q000166:cytology* / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D015233:Models, Statistical; D012306:Risk; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D004791:Enzyme Inhibitors; C080866:cagA protein, Helicobacter pylori