title: Shotgun sequencing analysis of Trypanosoma cruzi I Sylvio X10/1 and comparison with T. cruzi VI CL Brener.
abstract: Trypanosoma cruzi is the causative agent of Chagas disease, which affects more than 9 million people in Latin America. We have generated a draft genome sequence of the TcI strain Sylvio X10/1 and compared it to the TcVI reference strain CL Brener to identify lineage-specific features. We found virtually no differences in the core gene content of CL Brener and Sylvio X10/1 by presence/absence analysis, but 6 open reading frames from CL Brener were missing in Sylvio X10/1. Several multicopy gene families, including DGF, mucin, MASP and GP63 were found to contain substantially fewer genes in Sylvio X10/1, based on sequence read estimations. 1,861 small insertion-deletion events and 77,349 nucleotide differences, 23% of which were non-synonymous and associated with radical amino acid changes, further distinguish these two genomes. There were 336 genes indicated as under positive selection, 145 unique to T. cruzi in comparison to T. brucei and Leishmania. This study provides a framework for further comparative analyses of two major T. cruzi lineages and also highlights the need for sequencing more strains to understand fully the genomic composition of this parasite.
authors.affiliation: Science for Life Laboratory, Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden. oscar.franzen@scilifelab.se
authors.forename: Oscar
authors.initials: O
authors.lastname: Franzén
authors.forename: Stephen
authors.initials: S
authors.lastname: Ochaya
authors.forename: Ellen
authors.initials: E
authors.lastname: Sherwood
authors.forename: Michael D
authors.initials: MD
authors.lastname: Lewis
authors.forename: Martin S
authors.initials: MS
authors.lastname: Llewellyn
authors.forename: Michael A
authors.initials: MA
authors.lastname: Miles
authors.forename: Björn
authors.initials: B
authors.lastname: Andersson
mesh_terms: D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics*; D018503:Genome, Protozoan; D006801:Humans; D007843:Latin America; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017385:Sequence Homology; D026801:Synteny; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan