title: New primers for sex identification in the Chinese egret and other ardeid species.
abstract: Using the universal P2/P8 primers, we were able to obtain the gene segments of chromo-helicase-DNA binding protein (CHD)-Z and CHD-W from ten species of ardeid birds including Chinese egret (Egretta eulophotes), little egret (E. garzetta), eastern reef egret (E. sacra), great egret (Ardea alba), grey heron (A. cinerea), Chinese pond-heron (Ardeola bacchus), cattle egret (Bubulcus ibis), black-crowned night-heron (Nycticorax nycticorax), cinnamon bittern (Ixobrychus cinnamomeus) and yellow bittern (I. sinensis). Based on conserved regions inside the P2/P8-derived sequences, we designed new PCR primers for sex identification in these ardeid species. Using agarose gel electrophoresis, the PCR products showed two bands for females (140 bp derived from CHD-W and the other 250 bp from CHD-ZW), whereas the males showed only the 250 bp band. The results indicated that our new primers could be used for accurate and convenient sex identification in ardeid species.
authors.affiliation: Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zhou
authors.forename: Qingxian
authors.initials: Q
authors.lastname: Lin
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Fang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004247:DNA