title: Application of a peptide-mediated magnetic separation-phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples.
abstract: Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.
authors.affiliation: Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, Medical Biology Centre, Belfast, Northern Ireland, UK.
authors.forename: Antonio
authors.initials: A
authors.lastname: Foddai
authors.forename: Samuel
authors.initials: S
authors.lastname: Strain
authors.forename: Robert H
authors.initials: RH
authors.lastname: Whitlock
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Elliott
authors.forename: Irene R
authors.initials: IR
authors.lastname: Grant
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001431:Bacteriological Techniques / Q000379:methods*; D001435:Bacteriophages / Q000254:growth & development*; D002417:Cattle; D005243:Feces / Q000382:microbiology; D050296:Microbial Viability; D008892:Milk / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity