title: Effects of salvianolic Acid B on protein expression in human umbilical vein endothelial cells.
abstract: Salvianolic acid B (Sal B), a pure water-soluble compound extracted from Radix Salviae miltiorrhizae, has been reported to possess potential cardioprotective efficacy. To identify proteins or pathways by which Sal B might exert its protective activities on the cardiovascular system, two-dimensional gel electrophoresis-based comparative proteomics was performed, and proteins altered in their expression level after Sal B treatment were identified by MALDI-TOF MS/MS. Human umbilical vein endothelial cells (HUVECs) were incubated at Sal B concentrations that can be reached in human plasma by pharmacological intervention. Results indicated that caldesmon, an actin-stabilizing protein, was downregulated in Sal B-exposed HUVECs. Proteins that showed increased expression levels upon Sal B treatment were vimentin, T-complex protein 1, protein disulfide isomerase, tropomyosin alpha, heat shock protein beta-1, UBX domain-containing protein 1, alpha enolase, and peroxiredoxin-2. Additionally, Sal B leads to increased phosphorylation of nucleophosmin in a dose-dependent manner and promotes proliferation of HUVECs. We found that Sal B exhibited a coordinated regulation of enzymes and proteins involved in cytoskeletal reorganization, oxidative stress, and cell growth. Our investigation would provide understanding to the endothelium protection information of Sal B.
authors.affiliation: Department of Applied Cosmetology, Hungkuang University, Taichung 43302, Taiwan.
authors.forename: Tsong-Min
authors.initials: TM
authors.lastname: Chang
authors.forename: Guey-Yueh
authors.initials: GY
authors.lastname: Shi
authors.forename: Hua-Lin
authors.initials: HL
authors.lastname: Wu
authors.forename: Chieh-Hsi
authors.initials: CH
authors.lastname: Wu
authors.forename: Yan-Di
authors.initials: YD
authors.lastname: Su
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Wang
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Wen
authors.forename: Huey-Chun
authors.initials: HC
authors.lastname: Huang