title: [Expression of neuropeptides ghrelin and Nesfatin-1 in kainic acid kindling rats].
abstract: OBJECTIVE
  To observed the post-seizure expression level of neuropeptides ghrelin and Nesfatin-1 between the untreated and treated groups in kainic acid-kindling rats and understand the significance of their basic expressions.
  
  
  METHODS
  The male S-D rats were divided randomly into NS and VPA groups after seizure. Each group was divided into 7 subgroups of 3 h, 6 h, 12 h, 24 h, 3 d, 7 d and 14 d. And the blank group (without NS or VPA) and sham group (normal saline instead of kainic acid) were established. RIA (radioimmunoassay), ELISA (enzyme-linked immunosorbent assay) and immunohistochemistry were used to detect the expression levels of ghrelin and Nesfatin-1 in the serum and hypothalamus of kainic acid-kindling rats.
  
  
  RESULTS
  Compared with the blank and sham groups, the seizure rats had a decreased expression of ghrelin while there was an elevated expression of Nesfatin-1 in hypothalamus. This trend was consistent with the serum expressions of deacylated ghrelin and Nesfatin-1. The expression of ghrelin became elevated at all stages in the VPA group. Especially the subgroups of 24 h (0.320 ± 0.007) and 7 d (0.284 ± 0.004) had a significant increase (P < 0.05) while their expression levels remained lowed than that of the blank group (0.342 ± 0.039). The expressions of Nesfatin-1 had a significant decrease (P < 0.05) in all VPA subgroups while they were higher than those in the blank group (0.124 ± 0.007).
  
  
  CONCLUSIONS
  An accurate detection of serum expression level of deacylated ghrelin and Nesfatin-1 may reflect the fluctuation trend of neuropeptides in hypothalamus. It may offer a simple, sensitive and noninvasive method of diagnosing and treating epilepsy.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Ningxia Medical University, Key Lab of Craniocerbral Diseases, Yinchuan 750004, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Zong-zheng
authors.initials: ZZ
authors.lastname: Li
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Qi
authors.forename: Wen-zhong
authors.initials: WZ
authors.lastname: Xu
authors.forename: Pei-song
authors.initials: PS
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D054439:Ghrelin / Q000378:metabolism*; D007608:Kainic Acid / Q000009:adverse effects*; D007696:Kindling, Neurologic / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000080866:Nucleobindins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D054439:Ghrelin; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D007608:Kainic Acid