title: Measurement of macrophage growth and differentiation.
abstract: This unit provides protocols for measuring the abundance and growth of macrophage precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by either direct cell counting or by DNA measurement. Methods for the immunohistochemical identification of macrophages and the determination of their proliferative status in vivo by immunofluorescence are also included. It also describes methods for characterization of macrophage differentiation through the immunofluorescence analysis of cell-surface expression of CSF-1 receptor.
authors.affiliation: Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
authors.forename: Violeta
authors.initials: V
authors.lastname: Chitu
authors.forename: Yee-Guide
authors.initials: YG
authors.lastname: Yeung
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Yu
authors.forename: Sayan
authors.initials: S
authors.lastname: Nandi
authors.forename: E Richard
authors.initials: ER
authors.lastname: Stanley
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000379:methods*; D008264:Macrophages / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis
chemical_list: D016186:Receptor, Macrophage Colony-Stimulating Factor