title: Global gene response in Saccharomyces cerevisiae exposed to silver nanoparticles.
abstract: Silver nanoparticles (AgNPs), exhibiting a broad size range and morphologies with highly reactive facets, which are widely applicable in real-life but not fully verified for biosafety and ecotoxicity, were subjected to report transcriptome profile in yeast Saccharomyces cerevisiae. A large number of genes accounted for ∼3% and ∼5% of the genome affected by AgNPs and Ag-ions, respectively. Principal component and cluster analysis suggest that the different physical forms of Ag were the major cause in differential expression profile. Among 90 genes affected by both AgNPs and Ag-ions, metalloprotein mediating high resistance to copper (CUP1-1 and CUP1-2) were strongly induced by AgNPs (∼45-folds) and Ag-ions (∼22-folds), respectively. A total of 17 genes, responsive to chemical stimuli, stress, and transport processes, were differentially induced by AgNPs. The differential expression was also seen with Ag-ions that affected 73 up- and 161 down-regulating genes, and most of these were involved in ion transport and homeostasis. This study provides new information on the knowledge for impact of nanoparticles on living microorganisms that can be extended to other nanoparticles.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seongbuk-gu, Seoul, Republic of Korea.
authors.forename: Javed H
authors.initials: JH
authors.lastname: Niazi
authors.forename: Byoung-In
authors.initials: BI
authors.lastname: Sang
authors.forename: Yeon Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: Man Bock
authors.initials: MB
authors.lastname: Gu
mesh_terms: D002412:Cations; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D008668:Metallothionein / Q000235:genetics; D009152:Mutagenicity Tests; D025341:Principal Component Analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D012834:Silver / Q000494:pharmacology*
chemical_list: C500769:CUP1-1 protein, S cerevisiae; C562210:CUP1-2 protein, S cerevisiae; D002412:Cations; D029701:Saccharomyces cerevisiae Proteins; D012834:Silver; D008668:Metallothionein