title: Dictyostelium discoideum as a model system for identification of Burkholderia pseudomallei virulence factors.
abstract: Burkholderia pseudomallei is an emerging bacterial pathogen and category B biothreat. Human infections with B. pseudomallei (called melioidosis) present as a range of manifestations, including acute septicemia and pneumonia. Although melioidosis can be fatal, little is known about the molecular basis of B. pseudomallei pathogenicity, in part because of the lack of simple, genetically tractable eukaryotic models to facilitate en masse identification of virulence determinants or explore host-pathogen interactions. Two assays, one high-throughput and one quantitative, were developed to monitor levels of resistance of B. pseudomallei and the closely related nearly avirulent species Burkholderia thailandensis to predation by the phagocytic amoeba Dictyostelium discoideum. The quantitative assay showed that levels of resistance to, and survival within, amoeba by these bacteria and their known virulence mutants correlate well with their published levels of virulence in animals. Using the high-throughput assay, we screened a 1,500-member B. thailandensis transposon mutant library and identified 13 genes involved in resistance to predation by D. discoideum. Orthologs of these genes were disrupted in B. pseudomallei, and nearly all mutants had similarly decreased resistance to predation by D. discoideum. For some mutants, decreased resistance also correlated with reduced survival in and cytotoxicity toward macrophages, as well as attenuated virulence in mice. These observations suggest that some factors required by B. pseudomallei for resistance to environmental phagocytes also aid in resistance to phagocytic immune cells and contribute to disease in animals. Thus, D. discoideum provides a novel, high-throughput model system for facilitating inquiry into B. pseudomallei virulence.
authors.affiliation: Department of Microbiology, 210 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Hasselbring
authors.forename: Maharsh K
authors.initials: MK
authors.lastname: Patel
authors.forename: Mark A
authors.initials: MA
authors.lastname: Schell
mesh_terms: D000818:Animals; D016957:Burkholderia pseudomallei / Q000472:pathogenicity*; D004023:Dictyostelium / Q000469:parasitology*; D005260:Female; D057166:High-Throughput Screening Assays / Q000379:methods*; D054884:Host-Pathogen Interactions / Q000502:physiology*; D008554:Melioidosis / Q000235:genetics / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors