title: Comparative genomics of Escherichia coli strains causing urinary tract infections.
abstract: The virulence determinants of uropathogenic Escherichia coli have been studied extensively over the years, but relatively little is known about what differentiates isolates causing various types of urinary tract infections. In this study, we compared the genomic profiles of 45 strains from a range of different clinical backgrounds, i.e., urosepsis, pyelonephritis, cystitis, and asymptomatic bacteriuria (ABU), using comparative genomic hybridization analysis. A microarray based on 31 complete E. coli sequences was used. It emerged that there is little correlation between the genotypes of the strains and their disease categories but strong correlation between the genotype and the phylogenetic group association. Also, very few genetic differences may exist between isolates causing symptomatic and asymptomatic infections. Only relatively few genes that could potentially differentiate between the individual disease categories were identified. Among these were two genomic islands, namely, pathogenicity island (PAI)-CFT073-serU and PAI-CFT073-pheU, which were significantly more associated with the pyelonephritis and urosepsis isolates than with the ABU and cystitis isolates. These two islands harbor genes encoding virulence factors, such as P fimbriae (pyelonephritis-associated fimbriae) and an important immunomodulatory protein, TcpC. It seems that both urovirulence and growth fitness can be attributed to an assortment of genes rather than to a specific gene set. Taken together, urovirulence and fitness are the results of the interplay of a mixture of factors taken from a rich menu of genes.
authors.affiliation: DTU Food, Technical University of Denmark, DK-2800 Lyngby, Denmark.
authors.forename: Rebecca Munk
authors.initials: RM
authors.lastname: Vejborg
authors.forename: Viktoria
authors.initials: V
authors.lastname: Hancock
authors.forename: Mark A
authors.initials: MA
authors.lastname: Schembri
authors.forename: Per
authors.initials: P
authors.lastname: Klemm
mesh_terms: D058070:Asymptomatic Diseases; D055028:Comparative Genomic Hybridization; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D016680:Genome, Bacterial; D046228:Microarray Analysis; D010802:Phylogeny; D014552:Urinary Tract Infections / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors