title: Fine specificities of two lectins from Cymbosema roseum seeds: a lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide.
abstract: The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fucα1,2Galα1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the α(1,6) Man residue, the 3- and 4-hydroxyl group of the α(1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewis(x), Lewis(y), Lewis(a) and Lewis(b) epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, MI, USA.
authors.forename: Tarun K
authors.initials: TK
authors.lastname: Dam
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Celso S
authors.initials: CS
authors.lastname: Nagano
authors.forename: Bruno Am
authors.initials: BA
authors.lastname: Rocha
authors.forename: Raquel G
authors.initials: RG
authors.lastname: Benevides
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Luiz Ag
authors.initials: LA
authors.lastname: de Sousa
authors.forename: Stefan
authors.initials: S
authors.lastname: Oscarson
authors.forename: C Fred
authors.initials: CF
authors.lastname: Brewer
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D000818:Animals; D002846:Chromatography, Affinity; D004912:Erythrocytes / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry; D005643:Fucose / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D008358:Mannose / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D037121:Plant Lectins / Q000302:isolation & purification / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000017:ABO Blood-Group System; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D011134:Polysaccharides; D005643:Fucose; D008358:Mannose