title: Use of murine models to detect the allergenicity of genetically modified Lactococcus lactis NZ9000/pNZPNK.
abstract: By introducing aprN into Lactococcus lactis NZ9000, the genetically modified L. lactis NZ9000/pNZPNK successfully expressed the nattokinase. The safety assessment of this novel strain was based on allergenicity of pepsin digestion stability and murine model serologic identity. Subjecting to the GM strain and host to pepsin digestion, the soluble fractions and cell debris were fast degraded completely. Feeding with ovalbumin resulted in significantly higher production of IgG1 and IgE as compared to that of L. lactis NZ9000/pNZPNK or L. lactis NZ9000. Further, the serum IgG2a level increased dose-dependently at week 2 and induced immune reaction toward Th1 pathway. Secretion of cytokines IL-4 and IL-10 fed with lactococci was significantly lower than that of the OVA group. L. lactis NZ9000/pNZPNK did not increase the proliferation of type 2 helper T cells in spleen or induce allergenicity in BALB/c mice. On the basis of the results, the new GM lactic acid bacterium is regarded as safe to use.
authors.affiliation: Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Shen-Shih
authors.initials: SS
authors.lastname: Chiang
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Liu
authors.forename: Ting-Wei
authors.initials: TW
authors.lastname: Ku
authors.forename: Jeng-Leun
authors.initials: JL
authors.lastname: Mau
authors.forename: Hsin-Tang
authors.initials: HT
authors.lastname: Lin
authors.forename: Tzu-Ming
authors.initials: TM
authors.lastname: Pan
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007136:Immunoglobulins / Q000097:blood; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D051379:Mice; D030781:Organisms, Genetically Modified; D010434:Pepsin A / Q000378:metabolism; D013381:Subtilisins / Q000235:genetics / Q000276:immunology*
chemical_list: D000485:Allergens; D017931:DNA Primers; D007136:Immunoglobulins; D013381:Subtilisins; D010434:Pepsin A; C053771:nattokinase