title: Photoinduced nitric oxide release from a nitrobenzene derivative in mitochondria.
abstract: We report a novel NO donor (RpNO), containing a 2,6-dimethylnitrobenzene moiety for photocontrollable NO release and a rhodamine moiety for targeting to mitochondria. Photorelease of NO from RpNO in aqueous solution was confirmed by means of ESR analysis. Cellular release of NO from RpNO was confirmed with the aid of DAF-FM DA, an NO-specific fluorescence probe. RpNO was colocalized with MitoTracker Green FM, a mitochondrial stain, in HCT116 colon cancer cells and exhibited photodependent cytotoxicity. Our results indicate that RpNO is an effective NO donor for time-controlled, mitochondria-specific NO treatment.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Nagoya, Aichi 467-8603, Japan.
authors.forename: Taeko
authors.initials: T
authors.lastname: Horinouchi
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Fukuhara
authors.forename: Naoki
authors.initials: N
authors.lastname: Miyata
mesh_terms: D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000188:drug therapy; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008928:Mitochondria / Q000737:chemistry* / Q000528:radiation effects; D015394:Molecular Structure; D009569:Nitric Oxide / Q000737:chemistry / Q000494:pharmacology*; D009578:Nitrobenzenes / Q000737:chemistry*
chemical_list: C558764:1-iodo-3,5-dimethylbenzene; C558763:2,6-dimethylnitrobenzene; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009578:Nitrobenzenes; D009569:Nitric Oxide