title: Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles.
abstract: The present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K3[Fe(CN)6]/K4[Fe(CN)6] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1-14 ng/mL with a standard deviation of ±0.36 ng/mL. Finally, the biosensor was applied to a series of real milk samples.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 59100, Bornova-İzmir, Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinçkaya
authors.affiliation: Ege University, Faculty of Agriculture, Department of Dairy Technology, 59100, Bornova-İzmir, Turkey.
authors.forename: Özer
authors.initials: Ö
authors.lastname: Kınık
authors.affiliation: Namık Kemal University, Faculty of Arts and Sciences, Chemistry Department, Biochemistry Division, Değirmenaltı, Tekirdağ, Turkey.
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 59100, Bornova-İzmir, Turkey.
authors.forename: Çağrı
authors.initials: Ç
authors.lastname: Altuğ
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, 59100, Bornova-İzmir, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Akkoca
mesh_terms: D016607:Aflatoxin M1 / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D017097:Electric Impedance; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry
chemical_list: D016607:Aflatoxin M1; D006046:Gold; D004247:DNA