title: Supramolecular complexing of membane Siglec CD22 mediated by a polyvalent heterobifunctional ligand that templates on IgM.
abstract: We report the synthesis and in vitro evaluation of a multivalent homing device, a polymer which contains preordered pendant groups with dual specificity, a trisaccharide moiety, which is specific for the siglec CD22, and an antibody specific hapten, nitrophenol. The device efficiently attracts antihapten IgM to the surface of human lymphoma B cells as well as to CD22-conjugated magnetic beads by mediating the formation of a ternary complex on the surface of the target.
authors.affiliation: Department of Chemistry, Alberta Ingenuity Centre for Carbohydrate Science, University of Alberta , Edmonton, Alberta, Canada.
authors.forename: Lina
authors.initials: L
authors.lastname: Cui
authors.forename: Pavel I
authors.initials: PI
authors.lastname: Kitov
authors.forename: Gladys C
authors.initials: GC
authors.lastname: Completo
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.forename: David R
authors.initials: DR
authors.lastname: Bundle
mesh_terms: D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006801:Humans; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology*; D008024:Ligands; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000276:immunology; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D008280:Magnetics; D008863:Microspheres; D009596:Nitrophenols / Q000737:chemistry / Q000276:immunology; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000737:chemistry* / Q000276:immunology*; D013499:Surface Properties
chemical_list: D000936:Antigen-Antibody Complex; D006241:Haptens; D007075:Immunoglobulin M; D008024:Ligands; D046911:Macromolecular Substances; D009596:Nitrophenols; D051918:Sialic Acid Binding Ig-like Lectin 2