title: The effect of cytostatics and hyperthermia on RAJI human lymphoma cells.
abstract: OBJECTIVE
  To evaluate the effect of hyperthermia on cytostatic activity of chemotherapeutic drugs carboplatin, cisplatin, oxaliplatin, carmustine, gemcitabine and etoposide in human lymphoma cell culture.
  
  
  METHODS
  RAJI human lymphoma cells were incubated with cytostatics at 37 °C or 42 °C and evaluated for cell culture growth.
  
  
  RESULTS
  The number of viable cells after incubation with the drugs (except for gemcitabine) at 42 °C for 30 min was significantly lower than at 37 °C. There were synergism of cytostatic effects of platinum drugs (carboplatin, cisplatin and oxaliplatin) with cytostatic effect of 42 °C and the summation of cytostatic effect of carmustine or etoposide with the action of hyperthermia. The thermal enhancement ratio was 3.0 for oxaliplatin, 2.0 for cisplatin, 1.8-2.4 for carboplatin. The combination of platinum drugs with gemcitabine resulted in a significant enhancement of cytostatic activity.
  
  
  CONCLUSIONS
  Our findings suggest that a gain in sensitivity of RAJI human lymphoma cells to platinum drugs occurs at 42 °C.
authors.affiliation: N.N. Alexandrov National Cancer Center of Belarus, Minsk 223040, Belarus. istomin06@mail.ru
authors.forename: Yu P
authors.initials: YP
authors.lastname: Istomin
authors.forename: E A
authors.initials: EA
authors.lastname: Zhavrid
authors.forename: N V
authors.initials: NV
authors.lastname: Sachivko
authors.forename: E N
authors.initials: EN
authors.lastname: Alexandrova
authors.forename: P V
authors.initials: PV
authors.lastname: Pocheshinsky
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D054697:Cytostatic Agents / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans
chemical_list: D000970:Antineoplastic Agents; D054697:Cytostatic Agents