title: Effect of normobaric oxygen therapy in a rat model of intracerebral hemorrhage.
abstract: OBJECTIVE
  Normobaric oxygen (NBO) therapy may be neuroprotective in acute ischemic stroke. However, how NBO may affect intracerebral hemorrhage is unclear. We tested NBO in a rat model of striatal intracerebral hemorrhage.
  
  
  METHODS
  Intracerebral hemorrhage was induced by stereotactic injection of collagenase Type VII (0.5 U) into the right striatum of male Sprague-Dawley rats. One hour later, rats were randomized into controls (n=13) versus NBO treatment (n=13). NBO was applied for 2 hours. Hemorrhagic blood volume, brain water content, and neurological outcomes (forelimb placement test, forelimb asymmetry, neuroscore) were quantified at 72 hours. Experiments were repeated in a second independent laboratory to assess reproducibility in neurological outcomes (n=10 per group).
  
  
  RESULTS
  NBO did not worsen hemorrhage severity or brain edema. There were no significant differences in hemorrhagic blood volumes (control, 6.4±0.9 μL versus NBO, 7.0±2.1 μL; P=0.18) or brain water content (control, 81.9%±1.1% versus NBO, 81.6%±0.5%; P=0.58). NBO did not affect any of the neurological outcome tests in the primary or secondary studies.
  
  
  CONCLUSIONS
  NBO therapy may not worsen outcomes in intracerebral hemorrhage.
authors.affiliation: ACC-729-C, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Norio
authors.initials: N
authors.lastname: Fujiwara
authors.forename: Emiri T
authors.initials: ET
authors.lastname: Mandeville
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Geng
authors.forename: Yumin
authors.initials: Y
authors.lastname: Luo
authors.forename: Ken
authors.initials: K
authors.lastname: Arai
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.forename: Xunming
authors.initials: X
authors.lastname: Ji
authors.forename: Aneesh B
authors.initials: AB
authors.lastname: Singhal
authors.forename: Eng H
authors.initials: EH
authors.lastname: Lo
mesh_terms: D000818:Animals; D001810:Blood Volume / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000628:therapy*; D008297:Male; D023421:Models, Animal; D010100:Oxygen / Q000494:pharmacology; D010102:Oxygen Inhalation Therapy / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D010100:Oxygen