title: A proteomic approach to decipher chilling response from cold acclimation in pea (Pisum sativum L.).
abstract: Two pea lines (Pisum sativum L.) with contrasted behaviours towards chilling and subsequent frost were studied by a proteomic approach to better understand cold acclimation. Following a chilling period, the Champagne line becomes tolerant to frost whereas Terese remains sensitive. Variance analysis allowed to select 260 statistically variable spots with 68 identified proteins (35 in leaves, 18 in stems, and 15 in roots). These proteins were shared out in proteins related to chilling response or cold acclimation. The better adaptation of Champagne to chilling might be related to a higher content in proteins involved in photosynthesis and in defence mechanisms. Moreover Champagne might prevent freezing damage particularly thanks to a higher constitutive expression of housekeeping proteins related to Terese. After three days of subsequent frost, proteomes of previously chilled plants also showed significant differences compared to unchilled plants. Out of 112 statistically variable spots (44 in leaves, 38 in stems, and 30 in roots), 32 proteins were identified. These proteins were related to frost response or frost resistance. It seems that Champagne could resist to frost with the reorientation of the energy metabolism.
authors.affiliation: USTL INRA UMR 1281, Laboratoire des Stress Abiotiques et Différenciation des Végétaux Cultivés bât SN2, 3ème étage, Université des Sciences et Technologies de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Dumont
authors.forename: Nasser
authors.initials: N
authors.lastname: Bahrman
authors.forename: Estelle
authors.initials: E
authors.lastname: Goulas
authors.forename: Benoît
authors.initials: B
authors.lastname: Valot
authors.forename: Hélène
authors.initials: H
authors.lastname: Sellier
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Hilbert
authors.forename: Christophe
authors.initials: C
authors.lastname: Vuylsteker
authors.forename: Isabelle
authors.initials: I
authors.lastname: Lejeune-Hénaut
authors.forename: Bruno
authors.initials: B
authors.lastname: Delbreil
mesh_terms: D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome