title: Florfenicol impairs the immune responses to vaccination against foot-and-mouth disease in mice.
abstract: Florfenicol is a new type of broad-spectrum antibacterial that has been used in veterinary clinics. It showed immunosuppressive activity on the immune responses to vaccination against foot-and-mouth disease virus (FMDV) serotype O in mice. In the present study, BALB/c mice were immunized subcutaneously with FMDV serotype O antigen on days 1 and 14. Beginning on day 21, mice were treated with a single daily oral dose of florfenicol (50, 100, and 200 mg/kg) for seven consecutive days. On day 28, blood samples were collected to analyze FMDV-specific immunoglobulin G (IgG), IgG1, and IgG2b antibodies, and splenocytes were harvested to assess lymphocyte proliferation, CD3(+) T- and CD19(+) B-lymphocyte subsets. The results presented here demonstrated that florfenicol not only significantly suppressed concanavalin A-, lipopolysaccharide-induced splenocyte proliferation but also decreased the percentage of CD19(+) B-cells in a dose-dependent manner and suppressed CD3(+) T-cell at high doses. Moreover, FMDV-specific IgG, IgG1, and IgG2b antibody levels in FMDV-immunized mice were reduced by florfenicol. These results suggested that florfenicol could suppress humoral and cellular immune responses to vaccination against foot-and-mouth disease (FMD) in mice.
authors.affiliation: Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Guan
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Xue
authors.initials: X
authors.lastname: Shen
authors.forename: Wenhui
authors.initials: W
authors.lastname: Qian
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects*; D056724:Immunity, Humoral / Q000187:drug effects*; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014765:Viral Vaccines; C035534:florfenicol; D013839:Thiamphenicol