title: Developmental influence of the cellular prion protein on the gene expression profile in mouse hippocampus.
abstract: The conversion of the cellular prion protein (PrP(C)) to an abnormal and protease-resistant isoform is the key event in prion diseases. Mice lacking PrP(C) are resistant to prion infection, and downregulation of PrP(C) during prion infection prevents neuronal loss and the progression to clinical disease. These results are suggestive of the potential beneficial effect of silencing PrP(C) during prion diseases. However, the silencing of a protein that is widely expressed throughout the central nervous system could be detrimental to brain homeostasis. The physiological role of PrP(C) remains still unclear, but several putative functions (e.g., neuronal development and maintenance) have been proposed. To assess the influence of PrP(C) on gene expression profile in the mouse brain, we undertook a microarray analysis by using RNA isolated from the hippocampus at two different developmental stages: newborn (4.5-day-old) and adult (3-mo-old) mice, both from wild-type and Prnp(0/0) animals. Comparing the different datasets allowed us to identify "commonly" co-regulated genes and "uniquely" deregulated genes during postnatal development. The absence of PrP(C) affected several biological pathways, the most representative being cell signaling, cell-cell communication and transduction processes, calcium homeostasis, nervous system development, synaptic transmission, and cell adhesion. However, there was only a moderate alteration of the gene expression profile in our animal models. PrP(C) deficiency did not lead to a dramatic alteration of gene expression profile and produced moderately altered gene expression levels from young to adult animals. Thus, our results may provide additional support to silencing endogenous PrP(C) levels as therapeutic approach to prion diseases.
authors.affiliation: Laboratory of Prion Biology, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Trieste.
authors.forename: Stefano
authors.initials: S
authors.lastname: Benvegnù
authors.forename: Paola
authors.initials: P
authors.lastname: Roncaglia
authors.forename: Federica
authors.initials: F
authors.lastname: Agostini
authors.forename: Cristina
authors.initials: C
authors.lastname: Casalone
authors.forename: Cristiano
authors.initials: C
authors.lastname: Corona
authors.forename: Stefano
authors.initials: S
authors.lastname: Gustincich
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Legname
mesh_terms: D000367:Age Factors; D000818:Animals; D015153:Blotting, Western; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods*; D006624:Hippocampus / Q000378:metabolism*; D051379:Mice; D046228:Microarray Analysis; D017096:Prion Diseases / Q000235:genetics / Q000628:therapy*; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse