title: Abdominal subcutaneous fat gene expression and circulating levels of leptin and adiponectin in polycystic ovary syndrome.
abstract: OBJECTIVE
  To determine leptin and adiponectin serum levels and gene expression in subcutaneous adipose tissue from women with polycystic ovary syndrome (PCOS) and nonhirsute, ovulatory women; and leptin/adiponectin (L/A) ratio.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  University hospital gynecologic endocrinology unit.
  
  
  METHODS
  Thirty-one women with PCOS and 57 controls.
  
  
  METHODS
  Anthropometric, hormonal, and metabolic assessment; subcutaneous adipose tissue biopsy.
  
  
  METHODS
  Leptin and adiponectin serum levels, L/A ratio, controlled by age, and gene expression in women with PCOS and controls, stratified by body mass index and variables associated with androgen excess and insulin resistance.
  
  
  RESULTS
  Serum leptin was higher in overweight/obese patients with PCOS than in all normal-weight control women. Adiponectin levels were similar in all subgroups. The L/A ratio was lower in normal-weight controls (1.80; range 0.94-3.72) than in overweight/obese controls (5.27; range 2.66-13.58) and patients with PCOS (7.73; range 3.81-15.04). Subcutaneous leptin messenger RNA was higher in overweight/obese women with PCOS than in normal-weight controls (2.316 [range 1.987-2.580] vs. 1.687 [range 1.518-2.212]). Adiponectin gene expression was similar in all groups. Positive correlations were found between serum and messenger RNA levels for both leptin and adiponectin. On multiple regression analysis, percentage of body fat contributed significantly to L/A ratio in PCOS, independently of body mass index and free androgen index.
  
  
  CONCLUSIONS
  In PCOS, altered adipocyte secretion seems to relate to adiposity rather than to androgen excess.
authors.affiliation: Gynecologic Endocrinology Unit, Division of Endocrinology, Hospital de Clínicas de Porto Alegre, and Department of Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Sheila Bünecker
authors.initials: SB
authors.lastname: Lecke
authors.forename: Fabricio
authors.initials: F
authors.lastname: Mattei
authors.forename: Débora Martinho
authors.initials: DM
authors.lastname: Morsch
authors.forename: Poli Mara
authors.initials: PM
authors.lastname: Spritzer
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics; D050154:Adiposity / Q000235:genetics / Q000502:physiology; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology; D020738:Leptin / Q000097:blood* / Q000235:genetics; D009765:Obesity / Q000097:blood / Q000150:complications / Q000235:genetics; D050177:Overweight / Q000097:blood / Q000150:complications / Q000235:genetics; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000150:complications / Q000235:genetics*; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism*; D054928:Validation Studies as Topic; D055815:Young Adult
chemical_list: D052242:Adiponectin; D020738:Leptin