title: Role for Nox1 NADPH oxidase in atherosclerosis.
abstract: OBJECTIVE
  Examine the contribution of Nox1 NADPH oxidase to atherogenesis.
  
  
  RESULTS
  Male apolipoprotein E deficient mice (ApoE(-/-)) and male mice deficient in both apolipoprotein E and Nox1 (ApoE(-/-) Nox1(-/y)) received an atherogenic diet for 18 weeks. Mean blood pressures, body weights, and serum cholesterol levels were similar between the two groups of mice. Deficiency of Nox1 decreased superoxide levels and reduced lesion area in the aortic arch from 43% (ApoE(-/-)) to 28% (ApoE(-/-) Nox1(-/y)). The reduction in lesion size at the level of the aortic valve in ApoE(-/-)/Nox1(-/y) was accompanied by a decrease in macrophage infiltration as compared to ApoE(-/-) mice. Carotid artery ligation in ApoE(-/-) mice induced accelerated intimal hyperplasia with decreased cellular proliferation and increased collagen content in the neointima of vessels deficient in Nox1.
  
  
  CONCLUSIONS
  Nox1-derived ROS modify lesion composition and contribute to lesion size in a murine model of atherosclerosis.
authors.affiliation: Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, United States.
authors.forename: Andrea L
authors.initials: AL
authors.lastname: Sheehan
authors.forename: Samuel
authors.initials: S
authors.lastname: Carrell
authors.forename: Bryon
authors.initials: B
authors.lastname: Johnson
authors.forename: Bojana
authors.initials: B
authors.lastname: Stanic
authors.forename: Botond
authors.initials: B
authors.lastname: Banfi
authors.forename: Francis J
authors.initials: FJ
authors.lastname: Miller
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000201:enzymology* / Q000503:physiopathology; D002339:Carotid Arteries / Q000601:surgery; D049109:Cell Proliferation; D002784:Cholesterol / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D005260:Female; D006965:Hyperplasia / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000502:physiology*; D000074624:NADPH Oxidase 1; D013481:Superoxides / Q000737:chemistry
chemical_list: D001057:Apolipoproteins E; D013481:Superoxides; D003094:Collagen; D002784:Cholesterol; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1; C000614711:NOX1 protein, mouse