title: Expression of aldehyde dehydrogenase 6 reduces inhibitory effect of furan derivatives on cell growth and ethanol production in Saccharomyces cerevisiae.
abstract: Yeast dehydrogenases and reductases were overexpressed in Saccharomyces cerevisiae D452-2 to detoxify 2-furaldehyde (furfural) and 5-hydroxymethyl furaldehyde (HMF), two potent toxic chemicals present in acid-hydrolyzed cellulosic biomass, and hence improve cell growth and ethanol production. Among those enzymes, aldehyde dehydrogenase 6 (ALD6) played the dual roles of direct oxidation of furan derivatives and supply of NADPH cofactor to their reduction reactions. Batch fermentation of S. cerevisiae D452-2/pH-ALD6 in the presence of 2g/L furfural and 0.5 g/L HMF resulted in 20-30% increases in specific growth rate, ethanol concentration and ethanol productivity, compared with those of the wild type strain. It was proposed that overexpression of ALD6 could recover the yeast cell metabolism and hence increase ethanol production from lignocellulosic biomass containing furan-derived inhibitors.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Seong-Eon
authors.initials: SE
authors.lastname: Park
authors.forename: Hyun Min
authors.initials: HM
authors.lastname: Koo
authors.forename: Young Kyoung
authors.initials: YK
authors.lastname: Park
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Park
authors.forename: Jae Chan
authors.initials: JC
authors.lastname: Park
authors.forename: Oh-Kyu
authors.initials: OK
authors.lastname: Lee
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Park
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects*; D017931:DNA Primers; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005663:Furans / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D017931:DNA Primers; D005663:Furans; D000431:Ethanol; D000444:Aldehyde Dehydrogenase; C039281:furan