title: Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew.
abstract: Downy mildew, caused by the oomycete Plasmopara viticola, is one of the major threats to grapevine. All traditional cultivars of grapevine (Vitis vinifera) are susceptible to downy mildew, the control of which requires regular application of fungicides. In contrast, many sources of resistance to P. viticola have been described in the Vitis wild species, among which is V. amurensis Rupr. (Vitaceae), a species originating from East Asia. A genetic linkage map of V. amurensis, based on 122 simple sequence repeat and 6 resistance gene analogue markers, was established using S1 progeny. This map covers 975 cM on 19 linkage groups, which represent 82% of the physical coverage of the V. vinifera reference genetic map. To measure the general level of resistance, the sporulation of P. viticola and the necrosis produced in response to infection, five quantitative and semi-quantitative parameters were scored 6 days post-inoculation on the S1 progeny. A quantitative trait locus (QTL) analysis allowed us to identify on linkage group 14 a major QTL controlling the resistance to downy mildew found in V. amurensis, which explained up to 86.3% of the total phenotypic variance. This QTL was named 'Resistance to Plasmopara viticola 8' (Rpv8).
authors.affiliation: UMR 1131 Santé de la Vigne et Qualité du Vin, Institut National de la Recherche Agronomique, 28 rue de Herrlisheim, BP 20507, 68021, Colmar Cedex, France.
authors.forename: Paule
authors.initials: P
authors.lastname: Blasi
authors.forename: Sophie
authors.initials: S
authors.lastname: Blanc
authors.forename: Sabine
authors.initials: S
authors.lastname: Wiedemann-Merdinoglu
authors.forename: Emilce
authors.initials: E
authors.lastname: Prado
authors.forename: Ernst H
authors.initials: EH
authors.lastname: Rühl
authors.forename: Pere
authors.initials: P
authors.lastname: Mestre
authors.forename: Didier
authors.initials: D
authors.lastname: Merdinoglu
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D005202:Asia, Eastern; D008040:Genetic Linkage; D005819:Genetic Markers; D007113:Immunity, Innate; D018895:Microsatellite Repeats; D044742:Peronospora / Q000254:growth & development / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D040641:Quantitative Trait Loci; D027843:Vitis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D005819:Genetic Markers