title: Repair of alveolar cleft defect with mesenchymal stem cells and platelet derived growth factors: a preliminary report.
abstract: The purpose of this study was to evaluate the enhancing effect of recombinant platelet derived growth factor on human mesenchymal stem cells (hMSCs) in secondary alveoloplasty. Three patients with 4 alveolar defects were selected for this study. Mesenchymal stem cells were cultured from a posterior iliac bone aspirate. MSCs were mounted on biphasic scaffolds and combined with platelet derived growth factor (PDGF) in the operating room to make a triad of the scaffold, growth factor, and cells. The triads were placed in anterior maxillary cleft defects and closed with lateral advancement gingival flaps. The postoperative cleft bone volume was measured with cone beam computed tomography scans. A mean of 51.3% fill of the bone defect was calculated 3 months post-operation. Our data suggests the use of recombinant platelet derived growth factor with hMSCs may enhance the regeneration capacity of the cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Behnia
authors.forename: Arash
authors.initials: A
authors.lastname: Khojasteh
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Azita
authors.initials: A
authors.lastname: Tehranchi
authors.forename: Amir
authors.initials: A
authors.lastname: Atashi
mesh_terms: D000293:Adolescent; D000539:Alveolar Process / Q000002:abnormalities / Q000601:surgery; D000543:Alveoloplasty / Q000379:methods*; D001861:Bone Regeneration / Q000187:drug effects*; D018929:Cell Culture Techniques; D002648:Child; D002972:Cleft Palate / Q000150:complications; D005260:Female; D005337:Fibrin / Q000627:therapeutic use; D015870:Gene Expression; D006801:Humans; D006882:Hydroxyapatites; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D053495:Osteopontin / Q000096:biosynthesis / Q000235:genetics; D010865:Pilot Projects; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D054457:Tissue Scaffolds
chemical_list: D006882:Hydroxyapatites; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; C074950:hydroxyapatite-beta tricalcium phosphate; D015675:Osteocalcin; D053495:Osteopontin; D005337:Fibrin