title: Uncovering the post-embryonic functions of gametophytic- and embryonic-lethal genes.
abstract: An estimated 500-1 000 Arabidopsis (Arabidopsis thaliana) genes mutate to embryonic lethality. In addition, several hundred mutations have been identified that cause gametophytic lethality. Thus, a significant fraction of the ∼25,000 protein-coding genes in Arabidopsis are indispensable to the early stages of the diploid phase or to the haploid gametophytic phase. The expression patterns of many of these genes indicate that they also act later in development but, because the mutants die at such early stages, conventional methods limit the study of their roles in adult diploid plants. Here, we describe the toolset that allows researchers to assess the post-embryonic functions of plant genes for which only gametophytic- and embryonic-lethal alleles have been isolated.
authors.affiliation: Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Alicante, Spain.
authors.forename: Héctor
authors.initials: H
authors.lastname: Candela
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Pérez-Pérez
authors.forename: José Luis
authors.initials: JL
authors.lastname: Micol
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005804:Genes, Lethal; D017343:Genes, Plant; D005816:Genetic Complementation Test; D005838:Genotype; D055993:Germ Cells, Plant / Q000254:growth & development; D009154:Mutation; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000235:genetics* / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; C487568:RBR1 protein, Arabidopsis