title: Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication.
abstract: Homologous recombination (also termed homology-directed repair, HDR) is a major pathway for the repair of DNA interstrand cross-links (ICLs) in mammalian cells. Cells from individuals with Fanconi anemia (FA) are characterized by extreme ICL sensitivity, but their reported defect in HDR is mild. Here we examined ICL-induced HDR using a GFP reporter and observed a profound defect in ICL-induced HDR in FA cells, but only when the reporter could replicate.
authors.affiliation: Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
authors.forename: Koji
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavallo
authors.forename: Loïc
authors.initials: L
authors.lastname: Perrouault
authors.forename: Carine
authors.initials: C
authors.lastname: Giovannangeli
authors.forename: Mary Ellen
authors.initials: ME
authors.lastname: Moynahan
authors.forename: Marco
authors.initials: M
authors.lastname: Barchi
authors.forename: Erika
authors.initials: E
authors.lastname: Brunet
authors.forename: Maria
authors.initials: M
authors.lastname: Jasin
mesh_terms: D004261:DNA Replication; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans