title: Wounding systemically activates a mitogen-activated protein kinase in forage and turf grasses.
abstract: Forage and turf grasses are continually cut and grazed by livestock, however very little is known concerning the perception or molecular responses to wounding. Mechanical wounding rapidly activated a 46 kDa and a 44 kDa mitogen-activated protein kinase (MAPK) in six different grass species. In the model grass species Lolium temulentum, the 46 kDa MAPK was rapidly activated within 5 min of wounding both locally and systemically in an adjacent unwounded tiller. This indicates that wounding generates a rapidly propagated long-distance signal that activates a MAPK in the distal portions of the plant. This 46 kDa MAPK activity was not enhanced by the addition of the pathogen-associated signal salicylic acid (SA) to the wound site nor induced when exposed to methyl jasmonate (MJ), which is a potent inducer of the wound response in dicotyledonous plants. However, pretreatment with MJ increased the wound-induced activity of the 44 kDa MAPK over the activity in control plants.
authors.affiliation: USDA-ARS, National Forage Seed Production Research Center, 3450 SW Campus Way, Corvallis, OR 97331, USA. Jim.Dombrowski@ars.usda.gov
authors.forename: James E
authors.initials: JE
authors.lastname: Dombrowski
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Hind
authors.forename: Ruth C
authors.initials: RC
authors.lastname: Martin
authors.forename: Johannes W
authors.initials: JW
authors.lastname: Stratmann
mesh_terms: D000085:Acetates / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism; D006109:Poaceae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; D002374:Catalase; D020928:Mitogen-Activated Protein Kinases; D020156:Salicylic Acid