title: The interdependence of Ca2+ activation, sarcomere length, and power output in the heart.
abstract: Myocardium generates power to perform external work on the circulation; yet, many questions regarding intermolecular mechanisms regulating power output remain unresolved. Power output equals force × shortening velocity, and some interesting new observations regarding control of these two factors have arisen. While it is well established that sarcomere length tightly controls myocyte force, sarcomere length-tension relationships also appear to be markedly modulated by PKA-mediated phosphorylation of myofibrillar proteins. Concerning loaded shortening, historical models predict independent cross-bridge mechanics; however, it seems that the mechanical state of one population of cross-bridges affects the activity of other cross-bridges by, for example, recruitment of cross-bridges from the non-cycling pool to the cycling force-generating pool during submaximal Ca(2+) activation. This is supported by the findings that Ca(2+) activation levels, myofilament phosphorylation, and sarcomere length are all modulators of loaded shortening and power output independent of their effects on force. This fine tuning of power output probably helps optimize myocardial energetics and to match ventricular supply with peripheral demand; yet, the discernment of the chemo-mechanical signals that modulate loaded shortening needs further clarification since power output may be a key convergent point and feedback regulator of cytoskeleton and cellular signals that control myocyte growth and survival.
authors.affiliation: Department of Medical Pharmacology & Physiology, School of Medicine, University of Missouri, MA 415 Medical Sciences Building, Columbia, MO 65212, USA. mcdonaldks@missouri.edu
authors.forename: Kerry S
authors.initials: KS
authors.lastname: McDonald
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology; D006321:Heart / Q000033:anatomy & histology / Q000502:physiology*; D009200:Myocardial Contraction / Q000502:physiology*; D009218:Myosins / Q000378:metabolism; D010766:Phosphorylation; D012518:Sarcomeres / Q000502:physiology* / Q000648:ultrastructure*
chemical_list: D017868:Cyclic AMP-Dependent Protein Kinases; D009218:Myosins; D002118:Calcium