title: Antitumor activity of antimicrobial peptides against U937 histiocytic cell line.
abstract: We investigated cytotoxic activity of antimicrobial peptides of different origin (both naturally occurring and synthetic), structure and known mechanisms of action against human histiocytic lymphoma cell line U937. The strongest cytotoxic activity against U937 cell line was shown by Pexiganan MSI-78, followed by Citropin 1.1, Protegrin 1 and a synthetic lipopeptide, N-α-palmitoyl-L-lysyl-L-lysine amide (Pal-Lys-Lys-NH₂). The cytotoxic activity of the peptides was more dependent on the time of incubation than concentration. Only for the lipopeptide, whose mode of action was restricted to disruption of electric potential of the cell membrane, the correlation between cytotoxicity and concentration was almost linear. The high cytotoxicity of Pexiganan MSI-78, Protegrin 1 and the lipopeptide could be basically explained by their membranolytic activity leading to necrosis. However, in the case of Citropin 1.1, the cell membrane integrity was disrupted only slightly and independently of the peptide concentration. Therefore, some other mechanism of action might be responsible for its strong dose-dependent cytotoxic activity, e.g., membranolytic activity leading to apoptosis. Furthermore, TNF-α production due to LPS (lipopolysaccharide) stimulation was suppressed by the presence of Citropin 1.1, Pexiganan MSI-78 or Protegrin 1, but not by Buforin 2 or the lipopeptide. Our experiments have shown that cytotoxic activity is not limited to some specific molecular structure of a peptide, but rather to the length of the peptide chain as it is likely to affect the efficiency of the tumor cell membrane disruption and interaction with LPS.
authors.affiliation: Department of Cell Biology, Faculty of Medical Biotechnology, Medical University of Gdansk, Gdańsk, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Koszałka
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kamysz
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wejda
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kamysz
authors.forename: Jacek
authors.initials: J
authors.lastname: Bigda
mesh_terms: D029845:Amphibian Proteins / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D011506:Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D020298:U937 Cells
chemical_list: D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; C097849:buforin II; C121829:citropin 1.1 protein, Litoria; C094880:protegrin-1; C093828:pexiganan