title: Enhancement of imaging depth in turbid media using a wide area detector.
abstract: The depth of two-photon fluorescence imaging in turbid media can be significantly enhanced by the use of the here described fluorescence detection method that allows to efficiently collect scattered fluorescence photons from a wide area of the turbid sample. By using this detector we were able to perform imaging of turbid samples, simulating brain tissue, at depths up to 3 mm, where the two-photon induced fluorescence signal is too weak to be detected by means used in conventional two-photon microscopy.
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, Irvine, CA 92697-2715, USA.
authors.forename: Viera
authors.initials: V
authors.lastname: Crosignani
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Dvornikov
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
mesh_terms: D000362:Agar / Q000737:chemistry; D001921:Brain / Q000473:pathology / Q000648:ultrastructure; D001943:Breast Neoplasms / Q000473:pathology / Q000648:ultrastructure; D045744:Cell Line, Tumor; D003198:Computer Simulation; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods*; D012542:Scattering, Radiation; D012825:Silicon / Q000737:chemistry
chemical_list: D005780:Gelatin; D000362:Agar; D012825:Silicon