title: NMR-based metabolic profiling for serum of mouse exposed to source water.
abstract: (1)H nuclear magnetic resonance (NMR) based metabonomic method was used to characterize the profile of low-molecular-weight endogenous metabolites in mouse (Mus musculus) serum following exposure to Taihu Lake source water for 90 days. The (1)H NMR spectra of mice sera were recoded and a total of 21 metabolites were identified. Data reduction and latent biomarkers identification were processed by pattern recognition (PR) analysis. The principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) identified differences in metabolic profiles between control and treatment groups. A number of serum metabolic perturbations were observed in sera of source water treatment mice compared to control mice, including decreased levels of acetone, pyruvate, glutamine, lysine and citrate. These results indicated that Taihu Lake source water could induce energy metabolism changes in mouse related to fatty acid β-oxidation, tricarboxylic acid (TCA) cycle, citric acid cycle, and metabolism of some amino acids. (1)H NMR-based metabolic profiling provides new insight into the toxic effect of Taihu Lake source water, and suggests potential biomarkers for noninvasive monitoring of health risk.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu, 210046, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weixin
authors.initials: W
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.forename: Xuxiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Shupei
authors.initials: S
authors.lastname: Cheng
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D005618:Fresh Water / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D051379:Mice; D009930:Organic Chemicals / Q000032:analysis / Q000097:blood / Q000737:chemistry; D025341:Principal Component Analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000097:blood* / Q000737:chemistry
chemical_list: D009930:Organic Chemicals; D014874:Water Pollutants, Chemical