title: The biology of insecticidal activity and resistance.
abstract: Identifying insecticide resistance mechanisms is paramount for pest insect control, as the understandings that underpin insect control strategies must provide ways of detecting and managing resistance. Insecticide resistance studies rely heavily on detailed biochemical and genetic analyses. Although there have been many successes, there are also many examples of resistance that still challenge us. As a precursor to rational pest insect control, the biology of the insect, within the contexts of insecticide modes of action and insecticide metabolism, must be well understood. It makes sense to initiate this research in the best model insect system, Drosophila melanogaster, and translate these findings and methodologies to other insects. Here we explore the usefulness of the D. melanogaster model in studying metabolic-based insecticide resistances, target-site mediated resistances and identifying novel insecticide targets, whilst highlighting the importance of having a more complete understanding of insect biology for insecticide studies.
authors.affiliation: Department of Genetics, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria 3010, Australia.
authors.forename: Trent
authors.initials: T
authors.lastname: Perry
authors.forename: Philip
authors.initials: P
authors.lastname: Batterham
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Daborn
mesh_terms: D000691:Anabasine / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D003634:DDT / Q000494:pharmacology; D004026:Dieldrin / Q000494:pharmacology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D020868:Gene Silencing; D007300:Insect Control / Q000379:methods*; D007305:Insecticide Resistance / Q000187:drug effects / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology*; D023421:Models, Animal; D008958:Models, Molecular; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D034622:RNA Interference; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D007306:Insecticides; C082030:Rdl protein, Drosophila; D011963:Receptors, GABA-A; D011978:Receptors, Nicotinic; D003577:Cytochrome P-450 Enzyme System; D003634:DDT; D004026:Dieldrin; D000691:Anabasine