title: Protective effect of components isolated from Lindera erythrocarpa against oxidative stress-induced apoptosis of H9c2 cardiomyocytes.
abstract: Eight compounds were isolated from the methanol fraction of Lindera erythrocarpa and assessed for their ability to protect H9c2 cardiomyocytes against oxidative stress-induced cell death. Three of the compounds significantly reduced the release of lactate dehydrogenase from H9c2 cardiomyocytes treated with buthionine-[S,R]-sulfoximine and reduced the uptake of propidium iodide by these cells. These effects were concentration-dependent. The three inhibitory compounds were identified as (-)-epicatechin, avicularin and quercitrin by spectroscopic techniques including one- and two-dimensional NMR and mass spectroscopy.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Yi-Sook
authors.initials: YS
authors.lastname: Jung
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Kim
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Yang
authors.forename: Sohyun
authors.initials: S
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002392:Catechin / Q000494:pharmacology; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D027443:Lindera / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D011419:Propidium; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats
chemical_list: D005419:Flavonoids; D020011:Protective Agents; C012526:quercitrin; D011419:Propidium; D019328:Buthionine Sulfoximine; C041388:avicularin; D002392:Catechin; D011794:Quercetin; D007770:L-Lactate Dehydrogenase