title: The Burkholderia pseudomallei type III secretion system and BopA are required for evasion of LC3-associated phagocytosis.
abstract: Burkholderia pseudomallei is the causative agent of melioidosis, a fatal infectious disease endemic in tropical regions worldwide, and especially prevalent in southeast Asia and northern Australia. This intracellular pathogen can escape from phagosomes into the host cytoplasm, where it replicates and infects adjacent cells. We previously demonstrated that, in response to B. pseudomallei infection of macrophage cell line RAW 264.7, a subset of bacteria co-localized with the autophagy marker protein, microtubule-associated protein light chain 3 (LC3), implicating autophagy in host cell defence against infection. Recent reports have suggested that LC3 can be recruited to both phagosomes and autophagosomes, thereby raising questions regarding the identity of the LC3-positive compartments in which invading bacteria reside and the mechanism of the autophagic response to B. pseudomallei infection. Electron microscopy analysis of infected cells demonstrated that the invading bacteria were either free in the cytosol, or sequestered in single-membrane phagosomes rather than double-membrane autophagosomes, suggesting that LC3 is recruited to B. pseudomallei-containing phagosomes. Partial or complete loss of function of type III secretion system cluster 3 (TTSS3) in mutants lacking the BopA (effector) or BipD (translocator) proteins respectively, resulted in delayed or no escape from phagosomes. Consistent with these observations, bopA and bipD mutants both showed a higher level of co-localization with LC3 and the lysosomal marker LAMP1, and impaired survival in RAW264.7 cells, suggesting enhanced killing in phagolysosomes. We conclude that LC3 recruitment to phagosomes stimulates killing of B. pseudomallei trapped in phagosomes. Furthermore, BopA plays an important role in efficient escape of B. pseudomallei from phagosomes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia.
authors.forename: Lan
authors.initials: L
authors.lastname: Gong
authors.forename: Meabh
authors.initials: M
authors.lastname: Cullinane
authors.forename: Puthayalai
authors.initials: P
authors.lastname: Treerat
authors.forename: Georg
authors.initials: G
authors.lastname: Ramm
authors.forename: Mark
authors.initials: M
authors.lastname: Prescott
authors.forename: Ben
authors.initials: B
authors.lastname: Adler
authors.forename: John D
authors.initials: JD
authors.lastname: Boyce
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Devenish
mesh_terms: D000818:Animals; D001343:Autophagy; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D057131:Immune Evasion / Q000276:immunology*; D042541:Intracellular Space / Q000382:microbiology; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010587:Phagocytosis / Q000276:immunology*; D010588:Phagosomes / Q000378:metabolism / Q000382:microbiology; D021381:Protein Transport; D014617:Vacuoles / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D001426:Bacterial Proteins; D051907:Lysosomal Membrane Proteins; D008869:Microtubule-Associated Proteins; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1; C480860:Map1lc3b protein, mouse