title: Genetic systems for moderately halo(alkali)philic bacteria of the genus Methylomicrobium.
abstract: Biotechnologies for effective conversion of atmospheric greenhouse gases (CO(2) and CH(4)) into valuable compounds, such as chemical and petrochemical feedstocks or alternative fuels, offer promising new strategies for stabilization of global warming. A novel approach in this field involves the use of methanotrophic bacteria as catalysts for CH(4) conversion. In recent years, extremophilic methanotrophic species related to the genus Methylomicrobium have become favorable systems for bioprocess engineering, due to their high growth rates and tolerance of a wide range of environmental conditions and perturbations. While the cultures hold the potential of producing a broader range of chemicals from methane, the biotechnologies are still limited by the lack of reliable genetic approaches for system-level studies and strain engineering. In this chapter, we describe a set of molecular tools for genetic investigation and alteration of the Methylomicrobium spp.
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, Washington, USA.
authors.forename: David S
authors.initials: DS
authors.lastname: Ojala
authors.forename: David A C
authors.initials: DA
authors.lastname: Beck
authors.forename: Marina G
authors.initials: MG
authors.lastname: Kalyuzhnaya
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D008772:Methylococcaceae / Q000235:genetics*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012329:RNA, Bacterial