title: Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase.
abstract: Cinnamic acid production was demonstrated using Streptomyces as a host. A gene encoding phenylalanine ammonia lyase (PAL) from Streptomyces maritimus was introduced into Streptomyces lividans, and its expression was confirmed by Western blot analysis. After 4 days cultivation using glucose as carbon source, the maximal level of cinnamic acid reached 210 mg/L. When glycerol (30 g/L) was used as carbon source, the maximal level of produced cinnamic acid reached 450 mg/L. In addition, using raw starch, xylose or xylan as carbon source, the maximal level of cinnamic acid reached 460, 300, and 130 mg/L, respectively. We demonstrated that S. lividans has great potential to produce cinnamic acid as well as other aromatic compounds.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.forename: Takaya
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Takanori
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Michiru
authors.initials: M
authors.lastname: Miyake
authors.forename: Naoko
authors.initials: N
authors.lastname: Okai
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D002934:Cinnamates / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism*
chemical_list: D002934:Cinnamates; C029010:cinnamic acid; D010650:Phenylalanine Ammonia-Lyase; D005990:Glycerol