title: Phagocytic activity and pro-inflammatory cytokines production by the murine macrophage cell line J774A.1 stimulated by a recombinant BCG (rBCG) expressing the MSP1-C of Plasmodium falciparum.
abstract: Macrophages are involved in innate immunity against malaria due to their ability to phagocytose infected erythrocytes and produce inflammatory cytokines, which are important for controlling parasite growth during malaria infection. In this study, the ability of a recombinant BCG (rBCG) vaccine expressing the 19-kDa C-terminus of merozoite surface protein-1 (MSP1-C) of Plasmodium falciparum, to stimulate the phagocytic activity and secretion of pro-inflammatory cytokines by the macrophage cell line J774A.1 was measured at varying times. The results demonstrate the ability of the rBCG construct to activate the inflammatory action of macrophages, which is important as a first-line of defence in clearing malaria infections.
authors.affiliation: School of Health, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. rapeah@kck.usm.my
authors.forename: S
authors.initials: S
authors.lastname: Rapeah
authors.forename: M
authors.initials: M
authors.lastname: Dhaniah
authors.forename: A A
authors.initials: AA
authors.lastname: Nurul
authors.forename: M N
authors.initials: MN
authors.lastname: Norazmi
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology*; D020066:Merozoite Surface Protein 1 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology*; D010587:Phagocytosis; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins