title: [Genetic heterogeneity of heat shock protein synthesis as a factor determining the resistance of mammalia to stressors].
abstract: The relationship between the levels of 70 kDa family heat shock protein (Hsp) synthesis and lymphocyte sensitivity to stressors was investigated. Lymphocyte cultivation in mitogen deprived culture medium and (or) the cell treatment with alkylating agents have been used as a stress challenge. On the model of two inbred murine strains genetically contrasting by the sensitivity to alkylating agents we succeeded in demonstration that the basic level of Hsp synthesis depends on genotype. The quantity Hsp mRNA, as well as the intracellular level of the proteins were significantly higher in BALB/c than in C57BL/6 mice. The mice characterized by higher Hsp levels demonstrated higher resistance to alkylating agent action. The induction of surplus amount of Hsp by heat shock increased the cell resistance to the alkylating agent melphalan. Lymphocyte isolated from high Hsp producers, BALB/c mice, were more resistant to apoptotic signals induced by mitogen deprivation.
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004351:Drug Resistance / Q000187:drug effects* / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008558:Melphalan / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D013045:Species Specificity
chemical_list: D018906:Antineoplastic Agents, Alkylating; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D008558:Melphalan