title: Cytokine loaded biopolymers as a novel strategy to study stem cells during wound-healing processes.
abstract: The biopolymer matrigel loaded with cytokine can be used for the recruitment in vivo of specific cell populations and as a vector for the preparation of cell cultures. Data demonstrate that the injection of the matrigel biopolymer supplemented with interleukin-8 (IL-8) in the leech Hirudo medicinalis can be used to purify cell populations showing the same morphofunctional and molecular mechanisms of specific populations of vertebrate hematopoietic precursor cells involved in tissue repair. These cells spontaneously differentiated into myofibroblasts. This approach highlights how the innovative use of a cytokine-loaded biopolymer for an in vivo cell sorting method, applied to a simple invertebrate model, can be a tool for studying myofibroblast cell biology and its regulation, step by step.
authors.affiliation: Department of Biotechnology and Molecular Sciences, University of Insubria, Varese, Italy. annalisa.grimaldi@uninsubria.it
authors.forename: Annalisa
authors.initials: A
authors.lastname: Grimaldi
authors.forename: Serena
authors.initials: S
authors.lastname: Banfi
authors.forename: Jacopo
authors.initials: J
authors.lastname: Vizioli
authors.forename: Gianluca
authors.initials: G
authors.lastname: Tettamanti
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Noonan
authors.forename: Magda
authors.initials: M
authors.lastname: de Eguileor
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D047709:Hirudo medicinalis / Q000502:physiology*; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D023421:Models, Animal; D058628:Myofibroblasts / Q000166:cytology*; D061251:Primary Cell Culture; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D015703:Antigens, CD; D001704:Biopolymers; D004337:Drug Carriers; D004338:Drug Combinations; D016209:Interleukin-8; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen