title: Lactobacillus amylovorus DSM 19280 as a novel food-grade antifungal agent for bakery products.
abstract: Mould spoilage is the main cause of substantial economic loss in bakery industry and might also cause public health problems due to the production of mycotoxins. The reduction of mould growth in bakery products is thus of crucial importance and there is great interest to develop safe and efficient strategies for this purpose. In this study Lactobacillus amylovorus DSM19280 has been shown to produce a wide spectrum of antifungal compounds active against common bread spoilage fungi. Among the indicator moulds, Aspergillus fumigatus and Fusarium culmorum were the most sensitive organisms. Several antifungal compounds were found to be present in synthetic medium inoculated with L. amylovorus DSM19280 strain, some of them being reported here for the first time. Wheat doughs fermented with L. amylovorus DSM19280 had good rheological properties and the breads thereof were of high quality as shown by rheofermentometer and texture analyser measurements. The results were compared with those obtained with a control non-antifungal L. amylovorus DSM20531(T) strain, a non-acidified and a chemically acidified dough. The quality of sourdough and bread fermented with L. amylovorus DSM 19280 was comparable to that obtained by using L. amylovorus DSM20531 (T). Additionally, breads were evaluated for the ability to retard the growth of Fusarium culmorum FST 4.05, Aspergillus niger FST4.21, Penicillium expansum FST 4.22, Penicillium roqueforti FST 4.11 and fungal flora from the bakery environment. The biological preservation of bread with L. amylovorus DSM 19280 was also compared to the most commonly used antifungal agent Calcium propionate. Breads containing sourdough fermented with L. amylovorus DSM 19280 were more effective in extending the shelf life of bread than the calcium propionate.
authors.affiliation: School of Food Science, Food Technology and Nutrition, National University of Ireland, Cork, Ireland.
authors.forename: Liam A M
authors.initials: LA
authors.lastname: Ryan
authors.forename: Emanuele
authors.initials: E
authors.lastname: Zannini
authors.forename: Fabio
authors.initials: F
authors.lastname: Dal Bello
authors.forename: Agata
authors.initials: A
authors.lastname: Pawlowska
authors.forename: Peter
authors.initials: P
authors.lastname: Koehler
authors.forename: Elke K
authors.initials: EK
authors.lastname: Arendt
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001939:Bread / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005506:Food Contamination / Q000517:prevention & control*; D019649:Food Industry; D005519:Food Preservation / Q000379:methods*; D005658:Fungi / Q000187:drug effects*; D007779:Lactobacillus acidophilus / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011422:Propionates / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D011422:Propionates; C514136:calcium propionate