title: Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis.
abstract: Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
authors.affiliation: School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Ashelford
authors.forename: Maria E
authors.initials: ME
authors.lastname: Eriksson
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Allen
authors.forename: Rosalinda
authors.initials: R
authors.lastname: D'Amore
authors.forename: Mikael
authors.initials: M
authors.lastname: Johansson
authors.forename: Peter
authors.initials: P
authors.lastname: Gould
authors.forename: Suzanne
authors.initials: S
authors.lastname: Kay
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Millar
authors.forename: Neil
authors.initials: N
authors.lastname: Hall
authors.forename: Anthony
authors.initials: A
authors.lastname: Hall
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D057906:Circadian Clocks / Q000235:genetics*; D017124:Conserved Sequence; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000187:drug effects; D015203:Reproducibility of Results; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D029681:Arabidopsis Proteins; D009153:Mutagens; D005020:Ethyl Methanesulfonate