title: Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops.
abstract: OBJECTIVE
  To demonstrate the efficacy of Rho-associated kinase (ROCK) inhibitor Y-27632 for corneal endothelial wound healing both in in vitro and in vivo models.
  
  
  METHODS
  As an in vitro model, cultivated cynomolgus monkey corneal endothelial cells were scraped to create a linear defect. The wound distance was then determined during a 24-h culture in the presence or absence of 10 μM of Y-27632. As an in vivo model, central corneal endothelium of Japanese white rabbits was damaged by transcorneal freezing, then 10 mM of Y-27632 was applied topically six times daily for 48 h. The wound area of the corneal endothelium was evaluated after 48 h.
  
  
  RESULTS
  The mean wound distance in the cultured corneal endothelial cells was significantly shorter in the Y-27632 group than in the control group. In the rabbit model, the mean wound area of the Y-27632 group was significantly smaller than that of the control group.
  
  
  CONCLUSIONS
  This study demonstrated that ROCK inhibitor Y-27632 promotes corneal endothelial wound healing both in in vitro and in vivo.
authors.affiliation: Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Tatara, Kyotanabe 610-0321, Japan. nkoizumi@mail.doshisha.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Okumura
authors.forename: Noriko
authors.initials: N
authors.lastname: Koizumi
authors.forename: Morio
authors.initials: M
authors.lastname: Ueno
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Kana
authors.initials: K
authors.lastname: Hirata
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Torii
authors.forename: Junji
authors.initials: J
authors.lastname: Hamuro
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000577:Amides / Q000627:therapeutic use*; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D004728:Endothelium, Corneal / Q000293:injuries*; D004847:Epithelial Cells; D008252:Macaca fascicularis; D009883:Ophthalmic Solutions / Q000627:therapeutic use*; D011725:Pyridines / Q000627:therapeutic use*; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000577:Amides; D009883:Ophthalmic Solutions; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases