title: SYBR Green I-based real-time PCR targeting the rpoX gene for sensitive and rapid detection of Vibrio alginolyticus.
abstract: rpoX, a Vibrio alginolyticus specific stress regulating gene, was used to detect this fish pathogen by SYBR Green I-based real-time PCR. The specificity of the detection was confirmed in different samples. The minimum level of detection was 10(3) cells from pure culture and 10(2) cells from seawater.
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization, The South China Sea Institute of Oceanology, 164 Xingangxi Road, CAS, 510301 Guangzhou, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jing-jing
authors.forename: Chen
authors.initials: C
authors.lastname: Chang
authors.forename: Luo
authors.initials: L
authors.lastname: Peng
authors.forename: Ren
authors.initials: R
authors.lastname: Chun-hua
authors.forename: Jiang
authors.initials: J
authors.lastname: Xiao
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhe
authors.forename: Hu
authors.initials: H
authors.lastname: Chao-qun
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D052160:Benzothiazoles; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003959:Diamines; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology*; D009930:Organic Chemicals / Q000737:chemistry*; D010473:Perciformes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012808:Sigma Factor / Q000235:genetics; D044149:Vibrio alginolyticus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D052160:Benzothiazoles; D017931:DNA Primers; D004269:DNA, Bacterial; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; D012808:Sigma Factor; C098022:SYBR Green I