title: Multiple biological functions of novel basic proteins isolated from duck egg white: duck basic protein small 1 (dBPS1) and 2 (dBPS2).
abstract: Biological functions of duck basic protein small 1 (dBPS(1)) and 2 (dBPS(2)) were investigated by in vitro experiments. Results of agarose gel retardation assay indicated that dBPS(1) and dBPS(2) associate with RNA. Addition of NaCl or urea induced partial dissociation of dBPS(1)/dBPS(2)-RNA complex, implying that electrostatic interaction, hydrophobic interaction, and hydrogen bonds are involved in the association of dBPS(1)/dBPS(2) to RNA. dBPS(1) and dBPS(2) inhibited pancreatic lipase activity with the fifty percent inhibitory concentration (IC(50)) of 250 and 100 μg/mL, respectively. Peptic hydrolysates of dBPS(1) and those of dBPS(2) showed a potent angiotensin I-converting enzyme (ACE) inhibition with an IC(50) of 22.5 and 49.6 mg/L. The most potent ACE-inhibitory peptide was a nanopeptide (EKKGFCAGY) from dBPS(1) and an octapeptide (KYCPKVGY) from dBPS(2). These multiple biological functions of dBPS(1) and dBPS(2) may contribute to reducing the risk of lifestyle diseases.
authors.affiliation: Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Ikenobe, Miki, Kagawa, Japan.
authors.forename: Supaporn
authors.initials: S
authors.lastname: Naknukool
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004372:Ducks / Q000235:genetics / Q000378:metabolism*; D004527:Egg Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D004529:Egg White / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors; D008969:Molecular Sequence Data; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004527:Egg Proteins; D004791:Enzyme Inhibitors; D016601:RNA-Binding Proteins; D008049:Lipase