title: Tobacco consumption and benzo(a)pyrene-diol-epoxide-DNA adducts in spermatozoa: in smokers, swim-up procedure selects spermatozoa with decreased DNA damage.
abstract: OBJECTIVE
  To analyze the distribution of benzo(a)pyrene-diol-epoxide (BPDE)-DNA adducts in spermatozoa selected and nonselected by a swim-up procedure with relation to smoking habits.
  
  
  METHODS
  Comparative study.
  
  
  METHODS
  Public university and public university hospital.
  
  
  METHODS
  Seventy-nine men (37 smokers and 42 nonsmokers) who visited an infertility clinic for diagnostic.
  
  
  METHODS
  Tobacco and environmental exposure assessment, semen sample analysis, swim-up procedure, BPDE-DNA adduct immunolabeling.
  
  
  METHODS
  BPDE-DNA adduct quantification in selected (SEL-SPZ) and nonselected (NONSEL-SPZ) spermatozoa. Data were normalized by using a normalized fluorescence value (NFV).
  
  
  RESULTS
  The mean NFV (±SD) in SEL-SPZ was significantly higher in smokers than in nonsmokers (18.9±11.5 vs. 10.5±10.4, respectively). Within smokers, a paired analysis (SEL-SPZ and NONSEL-SPZ) showed that NFV was significantly lower in SEL-SPZ than in NONSEL-SPZ (20.0±11.3 vs. 31.5±16.0, respectively). Conversely, within nonsmokers, the mean NFV was higher in SEL-SPZ than in NONSEL-SPZ (10.3±10.6 vs 4.3±7.1, respectively).
  
  
  CONCLUSIONS
  Tobacco consumption is associated with BPDE-DNA adducts in spermatozoa. In smokers, semen processing by swim-up recovers potentially fertilizing spermatozoa that show a significantly lower amount of BPDE-DNA adducts compared with NONSEL-SPZ. Further study is needed to improve the spermatozoa selection in smoking patients requiring assisted reproductive technologies.
authors.affiliation: CECOS-Laboratoire de Biologie de la Reproduction, Marseille, France. jeanne.perrin@ap-hm.fr
authors.forename: Jeanne
authors.initials: J
authors.lastname: Perrin
authors.forename: Virginie
authors.initials: V
authors.lastname: Tassistro
authors.forename: Marion
authors.initials: M
authors.lastname: Mandon
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Grillo
authors.forename: Alain
authors.initials: A
authors.lastname: Botta
authors.forename: Irene
authors.initials: I
authors.lastname: Sari-Minodier
mesh_terms: D000328:Adult; D001564:Benzo(a)pyrene / Q000378:metabolism; D001578:Benzopyrans / Q000009:adverse effects / Q000378:metabolism*; D016022:Case-Control Studies; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D018736:DNA Adducts / Q000378:metabolism*; D004249:DNA Damage / Q000502:physiology; D004852:Epoxy Compounds / Q000009:adverse effects / Q000378:metabolism*; D006801:Humans; D008297:Male; D055101:Semen Analysis; D012907:Smoking / Q000378:metabolism*; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology*; D014026:Nicotiana
chemical_list: D001578:Benzopyrans; D018736:DNA Adducts; D004852:Epoxy Compounds; C056612:benzo(a)pyrene-DNA adduct; C482278:benzo(a,l)pyrene diol epoxide; D001564:Benzo(a)pyrene