title: Chemical composition and antimicrobial activity of the essential oil of endemic Dalmatian black pine (Pinus nigra ssp. dalmatica).
abstract: The chemical composition and the antimicrobial activity of the essential oil isolated from the needles of endemic Dalmatian black pine (Pinus nigra ssp. dalmatica) from Croatia were investigated. The chemical composition of the essential oil was determined by GC and GC/MS analyses, and the main compounds identified were α-pinene, β-pinene, germacrene D, and β-caryophyllene. Disc-diffusion and broth-microdilution assays were used for the in vitro antimicrobial screening. The Dalmatian black pine essential oil exhibited a great potential of antibacterial activity against Gram-positive bacteria (MIC=0.03-0.50% (v/v)) and a less pronounced activity against Gram-negative bacteria (MIC=0.12-3.2% (v/v)). The volatile compounds also inhibited the growth of all fungi tested, including yeast.
authors.affiliation: Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, Split 21000, Croatia. olivera@ktf-split.hr
authors.forename: Olivera
authors.initials: O
authors.lastname: Politeo
authors.forename: Mirjana
authors.initials: M
authors.lastname: Skocibusic
authors.forename: Ana
authors.initials: A
authors.lastname: Maravic
authors.forename: Mirko
authors.initials: M
authors.lastname: Ruscic
authors.forename: Mladen
authors.initials: M
authors.lastname: Milos
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D017523:Croatia; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028223:Pinus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013045:Species Specificity; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D009822:Oils, Volatile